&EPA
K^,pr°,ec,,°r Analytical Methods Approved for Compliance Monitoring under the
Ground Water Rule
Analysis for the following contaminants shall be conducted in accordance with the methods in the following table, or their equivalent as
determined by EPA. The methods and monitoring requirements for these contaminants are specified in 40 CFR 141.402(c)(2).
Additional methods are listed in Appendix A to Subpart C of Part 141.
The CFR is the legal reference for approved methods and takes precedent over this table. The table should accurately reflect the
analytical methods information published in 40 CFR 141. If discrepancies are found, please notify the Safe Drinking Water Hotline (800-
426-4791) so that EPA can correct the table.
§141.402(c)(2)- Analytical Methods
(1)	The time from sample collection to initiation of analysis may not exceed 30 hours. The ground water system is encouraged but is not required to hold
samples below 10°C during transit.
(2)	Collection of a standard sample volume of at least 100 mL for fecal indicator analysis regardless of the fecal indicator or analytical method used
(§141.402(b)(3))
(3)	Analysis of all ground water source samples using one of the analytical methods listed in the §141.402(c)(2) of this section for the presence of E.coli,
enterococci, or coliphage.
The procedures must be conducted with the documents listed at §141.402(c)(2) or one of the alternative methods listed in Appendix A to Subpart C of Part 141.
For Standard Methods Online, the year in which each method was approved by the Standard Methods Committee is designated by the last two digits following
the hyphen in the method number. The methods listed are the only online versions of the method that maybe used. For vendor methods, the date of the
method listed in §141.402(c)(2) or Appendix A to Subpart C of Part 141 is the date/version of the approved method. Laboratories should be careful to use only
the approved versions of the methods as product packaging inserts may not be the same as the approved versions of the methods.
(a)	Laboratory certification- systems must have all compliance samples required under this subpart analyzed by a laboratory certified by EPA or a primacy
state to analyze drinking water samples. The laboratory used by the system must be certified for each method (and associated contaminant(s)) used for
compliance monitoring analyses under this rule.
(b)	Incorporate by reference - The methods required in §141.402(c)(2) are incorporated by reference into the regulation.
Contaminant
Escherichia coli - Procedure (following Lactose Fermentation Methods):
Office of Water (MS - 4606 M)
EPA 821-F-17-003
February 2017

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Page 2 of 7
Method
Organization
Reference Title
Date
Notes
9221 F.l
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
1998
EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be
used for E.coli testing step after use of Standard Methods
9221B, 9221D, 9222B, or 9222C
9221 F.l
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 22nd Edition
2012
EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be
used for E.coli testing step after use of Standard Methods
9221B, 9221D, 9222B, or 9222C
9221 F.l-06
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
2006
EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be
used for E.coli testing step after use of Standard Methods
9221B, 9221D, 9222B, or 9222C
Contaminant
Escherichia coli -
3artition methods:

Method
Organization
Reference Title
Date
Notes

9222 G.lc(2)
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
1998
EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be
used for E.coli testing step after use of Standard Methods
9221B, 9221D, 9222B, or 9222C
Contaminant
Escherichia coli - membrane filtration methods:
Method
Organization
Reference Title
Date
Notes
1604
EPA
Method 1604: Total Coliforms
and Escherichia coli in Water by
Membrane Filtration Using a
Simultaneous Detection
Technique (Ml Medium),
September 2002
2002

m-
ColiBlue24®Test
Hach Company
Membrane Filtration Method m-
ColiBlue24® Broth, Revision 2,
August 17, 1999
1999


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Page 3 of 7
Method
Organization
Reference Title
Date
Notes
Chromocult
EMD Millipore
Chromocult® Coliform Agar
Presence/Absence Membrane
Filter Test Method for Detection
and Identification of Coliform
Bacteria and Escherichia coli for
Finished Waters, November
2000, Version 1.0
2000

Contaminant
Escherichia coli - enzyme substrate methods:
Method
Organization
Reference Title
Date
Notes
9223 B
Colilert®
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
1998

9223 B
Colilert®
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 21st Edition
2005

9223 B
Colilert®
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 22nd Edition
2012

9223 B-97
Colilert®
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
1997

9223 B-04
Colilert®
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
2004

9223 B
Colisure®
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
1998

9223 B
Colisure®
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 21st Edition
2005


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Page 4 of 7
Method
Organization
Reference Title
Date
Notes
9223 B
Colisure®
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 22nd Edition
2012

9223 B-97
Colisure®
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
1997

9223 B-04
Colisure®
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
2004

9223 B
Colilert-18®
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
1998

9223 B
Colilert-18®
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 21st Edition
2005

9223 B
Colilert-18®
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 22nd Edition
2012

9223 B-97
Colilert-18®
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
1997

9223 B-04
Colilert-18®
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
2004


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Page 5 of 7
Method
Organization
Reference Title
Date
Notes
E*Colite®
Charm Sciences
Charm E*Colite™
Presence/Absence Test for
Detection and Identification of
Coliform Bacteria and
Escherichia coli in Drinking
Water,
January 9,1998
1998

Readycult®
EMD Millipore
Readycult® Coliforms 100
Presence/Absence Test for
Detection and Identification of
Coliform Bacteria and
Escherichia coli in Finished
Waters,
January 2007,
Version 1.1
2007

Modified
Colitag®
CPI International, Inc.
Modified Colitag™ Test Method
for the Simultaneous Detection
of E. coli and other Total
Coliforms in water (ATP D05-
0035), August 28, 2009
2009

Tecta EC/TC
Veolia Water Solutions and
Technologies
Tecta™ EC/TC medium and the
Tecta™ Instrument: A
Presence/Absence Method for
Simultaneous Detection of Total
Coliforms and Escherichia coli (E.
coli) in Drinking Water, May 22,
2014, Version 1.0
2014

Contaminant
Enterococci - Mu
tiple Tube Technique methods:
Method
Organization
Reference Title
Date
Notes
9230 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
1998

9230 B-04
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
2004


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Page 6 of 7
Enterococci - membrane filtration methods:
Method
Organization
Reference Title
Date
Notes
9230 C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
1998

1600
EPA
EPA Method 1600: Enterococci
in Water by Membrane
Filtration Using membrane-
Enterococcus Indoxyl-P-D-
Glucoside Agar (mEI)
EPA 821-R-02-022
September 2002
2002

Enterococci - enzyme substrate methods:
Method
Organization
Reference Title
Date
Notes
Enterolert
Applied and Environmental
Microbiology
Budnick, G.E., R.T. Howard, D.R.
Mayo. 1996. "Evaluation of
Enterolert for Enumeration of
Enterococci in Recreation
Waters". Applied and
Environmental Microbiology.
62:3881-3884.
1996

Contaminant
Coliphage:
Method
Organization
Reference Title
Date
Notes
1601
EPA
EPA 1601: Male-specific (F+) and
Somatic Coliphage in Water by
Two-step Enrichment
Procedure,
April 2001
2001

1602
EPA
EPA 1602: Male-specific (F+) and
Somatic Coliphage in Water by
Single Agar Layer (SAL)
Procedure,
April 2001
2001


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Page 7 of 7
Method
Organization
Reference Title
Date
Notes
Fast Phage®
Charm Sciences
Fast Phage Test
Presence/Absence for Coliphage
in Ground Water with Same Day
Positive Prediction, ATP Case
No. D09-0007, Version 009,
November 28, 2012
2012


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