JV Unitod States
Environmental Protection
M mAgoncy
Annual Report 2015
EPA 910-R-17-001
U.S. Environmental Protection Agency
Regional Laboratory Network

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Cover: Cover photos were obtained from EPA Office of Multimedia intranet site.

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Table of Contents
Page
Executive Summary		ii
Section I — U.S. EPA Regional Laboratories/Regional Laboratory Network: An Overview		1
U.S. EPA Regional Laboratories 		2
Regional Laboratory Network: An Overview		4
Section II — Regional Laboratory Network Highlights		7
Protecting Water: A Precious, Limited Resource 		8
Addressing Climate Change and Improving Air Quality 		13
Taking Action on Toxics and Chemical Safety 		15
Making a Visible Difference in Communities across the Country		18
Launching a New Era of State, Tribal, and Local Partnerships		20
Embracing EPA as a High-Performing Organization		22
Working toward a Sustainable Future 		25
Section III — FY2015 Laboratory Accomplishment Results Summary 		27
Section IV— Regional Laboratories Capabilities Tables — FY2015 		31
Regional Laboratories Core Capabilities — FY2015		32
Regional Laboratories Unique Capabilities — FY2015 		35
Regional Laboratories Developing Capabilities — FY2015 		45
I
Appendix A — Acronyms/Abbreviations	 A-1
List of Illustrations
Figure	Page
1.	Analytical Support to EPA Programs in FY2015 		28
2.	FY2015 U.S. EPA Regional Laboratories Analyses Provided by Program		28
3.	Projects/Sites Supported by Regional Laboratory Data FY2015 by Program Element 		29
4.	Method Development Project Support to EPA Programs in FY2015 		29

EPA-910-R-17-001

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Executive Summary

The U.S. Environmental Protection Agency (U.S. EPA) Regional Laboratories are state-of-the-art, full-service
environmental laboratories delivering analytical services, field support, quality assurance and data review, and expert
technical assistance. Ten Regional Laboratories individually support the 10 EPA regions while also collaborating to
form a highly effective Regional Laboratory Network (RLN). This report highlights the diversity of support and
capabilities, all of which reinforce EPA's mission and ongoing priorities.
In Fiscal Year 2015 (FY2015), Regional Laboratories performed over 150,000 analyses to support agency priorities
and to solve emerging environmental issues. This analytical work supported activities related to over 1,700 sites
and projects associated with a wide range of agency programs.
One particular environmental challenge, Gold King Mine became a multi-
regional response where mine drainage from Silverton, Colorado caused
mine water to discharge to Cement Creek, a tributary to Animas River.
Region 6, Region 8 and Region 9 were all involved in the response and
Regional Laboratories were critical in providing analysis, data review and
quality assurance support.
To enhance collaboration between regional, program and research and
development laboratories, the Laboratory Enterprise Forum was
formed in FY2015 to provide a mechanism to work across the agency
laboratory system, to identify approaches to enhance the efficient use of
facilities, and to evaluate tools promoting high-performance organizations.
Emerging contaminants continue to be a concern for the agency and Regional Laboratories continue to evaluate
methods and analytical technologies to develop approaches to meet the demands for detection. For FY2015,
analyzing for perfluorinated compounds (PFCs) and new testing tools for cyanotoxins were of significant interest.
Accomplishments presented in this report capture only a few of the overall activities provided by all Regional
Laboratories. These accomplishments underscore the commitment of RLN to be an integral part in protecting
human health and the environment.
EPA Agency Themes Meeting the Challenge Ahead
II
Protecting Water: A Precious, Limited Resource
Addressing Climate Change and Improving Air Quality
Taking Action on Toxics and Chemical Safety
Making a Visible Difference in Communities across the Country
Launching a New Era of State, Tribal, and Local Partnerships
Embracing EPA as a High-Performing Organization
Working Toward a Sustainable Future

ii
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U.S. EPA Regional Laboratory System
FY2015 Annual Report

Section I — U.S. EPA Regional Laboratories/Regional Laboratory
Network: An Overview
I
vvEPA
United States
Environmental Protection
Agoncy
i3A-i 0 R-17- 0

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
U.S. EPA Regional Laboratories
Region 1:
New England Regional Laboratory Investigation
& Analysis Branch
Ernest Waterman, Director
Waterman. Ernest@epa.gov
11 Technology Drive
N. Chelmsford, MA 01863-2431
Phone: 617-918-8632
FAX: 617-918-8540
Region 2: Division of Environmental Science and
Assessment Laboratory Branch
John Bourbon, Director
bourbon.iohn@epa.gov
2890 Woodbridge Ave.
Edison, NJ 08837
Phone: 732-321-6706
Fax: 732-321-6165
Region 3: Environmental Science Center Laboratory Branch
Karen Costa, Manager
Costa.Karen@epa.gov
701 Mapes Road
Ft. Meade, MD 20755-5350
Phone: 410-305-2689
Fax: 410-305-3095
Region 4: Analytical Support Branch
Danny France, Director
France.Dannv@epa.gov
980 College Station Road
Athens, GA 30605-2720
Phone: 706-355-8551
Fax: 706-355-8803
Region 5: U.S. EPA Region 5 Laboratory, Chicago Regional
Laboratory
Dennis Wesolowski, Director
wesolowski.dennis@epa.gov
536 S. Clark Street
Chicago, IL 60605
Phone: 312-353-9084
Fax: 312-886-2591

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
U.S. EPA Regional Laboratories
Region 6:
Region 7:
Region 8:
Region 9:
Region 10:
Environmental Services Branch
Wes McQuiddy, Director
Mcaulddv.Davld@epa.gov
10625 Fallstone Road
Houston, TX 77099
Phone: 214-665-6722
Fax: 281-983-2124
Regional Science & Technology Center
Margie St. Germain, Director
Staermain.maraie@epa.gov
300 Minnesota Ave.
Kansas City, KS 66101
Phone:913-551-5154
Fax: 913-551-7873
U.S. EPA Region 8 Laboratory
Mark Burkhardt, Director
Burkhardt.Mark@epa.gov
16194 West 45th Drive
Golden, CO 80403
Phone: 303-312-7799
Fax: 303-312-7800
U.S. EPA Region 9 Laboratory
Pete Husby, Director
husbv.peter@epa.qov
1337 S. 46th Street, Bldg. 201
Richmond, CA 94804-4698
Phone: 510-412-2311
Fax: 510-412-2302
Manchester Environmental Laboratory
Barry Pepich, Director
Pepich.Barrv@epa.gov
7411 Beach Drive East
Port Orchard, WA 98366
Phone: 360-871-8701
Fax: 360-871-8747
EPA-910-R-17-001

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
EPA's Regional Laboratory Network: An Overview
m

EPA has 10 regional offices and each region has a laboratory The
Regional Laboratories provide mission-critical support to the Agency,
protecting human health and the environment. Service and expertise
provided by each Regional Laboratory are tailored to meet the needs of
that particular region or program and to address complex and emerging
environmental issues. In addition to
supporting each region, the 10 Regional
Laboratories collaborate to form the
Regional Laboratory Network (RLN).
Efficiency, effectiveness, and flexibility are
maximized by using scientific expertise,
implementing and developing methods,
and maximizing partnerships within the
network and across the nation.
Services tailored to meet
regional needs and to
address complex and
emerging environmental
issues
Environmental decisions and policies
provide the RLN with the analytical
structure to meet program needs.
Regional Laboratories also provide support
to national initiatives and research. Each
laboratory within the RLN constantly and consistently meets and supports
project-specific objectives, achieves quality goals, provides analytical
expertise, and produces accurate data within the Agency.
Support special project-
specific objectives and
goals towards a
sustainable future

EPA Regional Laboratories are committed
to producing quality data. The laboratories
follow EPA organizational directives for a
high-performing organization. All 10
laboratories are accredited by National or
International Accreditation programs
ensuring effective quality systems,
improved performance, and defensible
data. External assessments are
performed regularly at RLN laboratories.
Accreditation following
National Environmental
Laboratory Accreditation
Conference (NELAC) or
International Standards
Organization (ISO) 17025
Contracting mechanisms are used within the RLN to provide additional
procurement of analytical services. The Contract Laboratory Program
(CLP) provides standard analytical methods
supporting the Superfund Program. Each
laboratory uses an Environmental Services
Assistance Team (ESAT), which is a
contract to support laboratory functions.
RLN supports Agency
quick responses to
emergencies
This organizational structure permits EPA Regional Laboratories to
provide quick response to emergencies, while providing timely completion
of all projects. During FY2015, 10 Regional Laboratories supported over
150,000 sample analyses and over 1,700 projects.
EPA-910-R-17-001

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
EPA Regional Laboratories provided analytical responses to
approximately 15 significant emergency response events in FY2015
including the Gold King Mine response. EPA Regional Laboratories
are capable of analyzing samples
suspected to contain a variety of
chemical and biological compounds,
including chemical warfare agents.
Also, some Regional Laboratories
developed and validated new methods
for perfiuorinated organic compounds, Cyanotoxins and other
compounds that characterize and remediate contaminated areas.
Respond to Homeland
Security events
Within each Regional Laboratory, core capabilities allow support to
various EPA programs. Unique capabilities provide the flexibility for
each laboratory to meet geographical environmental demands or
regional and national initiatives. Three
tables (core, unique, and developing)
summarize chemical, physical and
biological/microbiological capabilities for
each region. The Core, Unique, and
Developing capabilities tables for each
Regional Laboratory are provided in Section IV and are available on
the following EPA websites.
Core, Unique, and
Developing capabilities
span across RLN
Regional Laboratory scientists are certification officers for the Drinking
Water Laboratory Certification Program and participate in state
drinking water audit programs.
Laboratory scientists also provide
management, technical, logistical, and
oversight support to EPA, State and
tribal programs, operate air monitoring
quality assurance programs, and
support field sampling functions.
Serve crucial roles in
regional drinking water
audit programs
Regional Laboratory scientists are a valuable resource. Scientists
have expertise in analytical methods, quality assurance and quality
control principles, data validation, field analytical techniques, and
solving complex analytical problems.
During FY2015, the 10 Regional
Laboratories supported more than
125 method improvement projects.
Support analytical
method improvements
EPA's Regional Laboratory Network: An Overview
Regional Laboratories Core Capabilities — FY2015
https://www.epa.gov/regionallabs
Regional Laboratories Unique Capabilities — FY2015
http://www.epa.gov/regionallabs/epa-regional-laboratories-unigue-analvtical-capabilities-and-documentation-region
Regional Laboratories Developing Capabilities — FY2015
http://www.epa.gov/measurements/collection-methods
EPA-910-R-17-001
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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Section li — Regional Laboratory Network Highlights

Ok	J\ United States
Environmental Protection
!¦! #mAgoncy
EPA-910-R-17-001
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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Protecting Water: A Precious, Limited Resource
Regional Laboratories play an important role to ensure drinking water is safe, to restore and
maintain oceans, watersheds, and their aquatic ecosystems to protect human health, to support
economic and recreational activities, and to provide healthy habitat for fish, plants, and wildlife.
\
FY2015 HIGHLIGHTS
ENSURE SAFE DRINKING WATER
GOLD KING MINE RESPONSE
MEASURING CYANOTOXINS
Regional Laboratories play ari important part in
protecting and restoring the nation's water resources by
providing:
•	key data for regions and their partners and target
actions to protect human health and aquatic
ecosystems more efficiently
•	technical and regulatory support to drinking water
laboratories and training and support for water
quality monitoring efforts
•	analytical support for various projects across the
U.S.
Ensuring Safe Drinking Water
Laboratories that analyze drinking water samples
are required to be certified by an approved
certifying authority. The EPA Regional
Laboratory personnel and trained certification
officers conduct on-site evaluations of drinking
water laboratories operated by states and tribal
communities. Certification Officers also conduct
audits of state certification programs to ensure all
laboratories analyzing drinking water samples are
following approved methods mandated by EPA's
National Primary Drinking Water Regulations.
Ultimately, the effort of the laboratory certification
program ensures drinking water is free from
harmful contaminants.
Regional Laboratories Supported
223
Compliance Reinforcement related Drinking Water Projects
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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Protecting Water: A Precious, Limited Resource
Gold King Mine Response
In August 2015, a plug in the Gold King Mine breached sending yellow-orange colored water into Cement Creek,
a tributary of the Animas River in Colorado, which then ran into the San Juan River in northwestern New
Mexico. Overall response coordination was implemented with Region 8, Region 6, and Region 9. EPA Region
6 emergency response crews began collecting samples for i	i
drinking water to assess possible mine waste leaching into local
groundwater wells near the river.	- '*
Region 6 and Region 8 Laboratories provided analytical support
to confirm results obtained from commercial laboratories.
Analysis required expedited turnaround time, with final reports
submitted within 48 hours. Analysis included metals, mercury,
and calculated hardness. Based on Region 6 and Region 8
Laboratories, confirmation results provided focused resampling,
which yielded usable metal results that previously indicated
abnormalities.
Updates on continued efforts are provided on EPA's website at:
www.epa.gov/goldkingmine
District of Columbia (DC)
Drinking Water Emergency
Mid-December 2015, after a "Do Not Drink" advisory
was issued for portions of the District of Columbia's
neighborhoods when petroleum was detected in tap
water, Region 3 Laboratory was called upon to provide
screening and confirmatory analysis for a "petroleum or
tar-like" substance. Based on the Region 3 Laboratory
results DC Aqueduct was able to lift the "do not drink"
restriction.
EPA-910-R-17-001
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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Protecting Water: A Precious, Limited Resource
New Detection Methods for
Cyanotoxins arid Algal
Blooms
Cyanobacteria or blue-green algae occur around the
world in nutrient-rich water environments. Some of
these cyanobacteria produce toxins that are harmful
to humans and animals. Humans and animals can
be exposed to these harmful toxins by several
pathways, including ingestion, inhalation, and
contact with the skin (bathing and/or recreation) in
the effected waters. These toxins impacted over
500,000 people in Toledo, Ohio when citizens were
ordered to not drink their water. Some of these
toxins are being detected in surface waters in
Region 8 arid elsewhere in the United States (U.S.).
Low-level chronic exposure to mixtures of these
chemicals can have adverse ecological or human
health effects. For example, new (2015) United
States Environmental Protection Agency (USEPA)
Health Advisories (HAs) have recommended at, or
below 0.3 micrograms per liter for microcystins and
0.7 micrograms per liter for cylindrospermopsin in
drinking water for children of pre-school age and
younger (less than 6 years old). For school-age
children through adults, the recommended HA levels
for drinking water are at, or below 1.6 micrograms
per liter for microcystins and 3.0 micrograms per liter
for cylindrospermopsin.
Data from both the Assessment and Management
Strategic Plan for Drinking Water and the Harmful
Algal Bloom arid Hypoxia Research arid Control Act
are used in the Regions, States, and Municipalities
to assess their drinking water and recreational water
facilities. This coordination improves our scientific
understanding of the fate, transport, and affects from
algal toxin exposure, and regional and national water
quality initiatives. The analysis of waters affected by
algal blooms also provided timely data for making
local public health risk decisions. This teamwork-
based effort is improving and maintaining
improvements in water quality as well as fostering
partnerships within the agency, between the regional
states and other federal agencies.
The Drinking Water Unit from the Office of
Partnerships & Regulatory Assistance (OPRA), the
Water Quality Unit from the Office of Ecosystems,
Protection and Remediation (EPR), arid the
Laboratory Services Program from the Office of
Technical and Management Services worked
together to identify and develop the algal toxin
analysis methods. This coordination resulted in the
development of two analytical methods to monitor
for four individual toxins, and one field screening
method. Data collected from three regional states
and one municipality were shared with National
Oceanic and Atmospheric Administration (NOAA),
as well as, EPA's Office of Research and
Development (ORD). Expansion of the analytical
methods (new analytes) and laboratory sample
analysis capacity are planned for 2016.
Regional Laboratories provide cyanotoxin
testing and develop methods for improved
detection
10
EPA-910-R-17-001

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Protecting Water: A Precious, Limited Resource
Microbial Source Tracking
Of the 138 watersheds in Island County, Washington,
67 have been tested and 60% exceed Washington's
water quality standards for fecal coliform. Exceeding
fecal coliform standards requires beach closures and
fishing restrictions. These exceedances negatively
affect shellfish harvesting areas, public beaches, lakes,
and streams bearing salmon listed as endangered
under the Endangered Species Act (ESA). This impact
on commercial and recreational shellfish opportunities
has significantly affected economic resources to Island
County.
Since the summer of 2014, Region 10 Laboratory, in
coordination with local county health officials, has
supported studies to identify sources of this pollution
using microbial source tracking (MST) analyses on
surface waters. MST is capable of differentiating
ruminant from human fecal contamination. The goal of
these studies is to help improve water quality by
identifying the source of contamination in the three
identified watersheds, reopen shellfish harvest areas,
improve beaches for water recreation, and improve the
habitat for native aquatic species. Once the
contamination source is identified, Island County will
move forward with corrective actions.
Cherokee Water Quality and
Biological Assessment
Region 4 Laboratory, in collaboration with the Eastern
Band Cherokee Indians (EBCI), conducted stream
assessments to provide updates on the condition of the
environmental, recreational, and cultural quality, and the
significance of the Hiawassee and Valley River
Watershed streams. Many recreational activities, such
as maintaining native trout species and supporting trout
hatchery fishing, are supported in these streams.
Existing biological data records date back to 1988,
attesting to excellent water quality conditions. The last
assessment of these streams was conducted in 2005,
with data indicating sustainable water quality conditions
and possibly improvement. The EBCI Tribe requested
assistance from Region 4 in conducting similar
assessments.
Proctor Creek Watershed
Proctor Creek, a 9-mile stream that flows from
downtown Atlanta, Georgia, to the Chattahoochee
River, is listed for impairment due to fecal coliform
bacteria. Collaboration between the Region 4
Laboratory, Region 4 Water Protection Division (WPD),
and community groups led to the development of the
monitoring plan, which addresses data gaps for Proctor
Creek. The planned monitoring event data will allow
identification of contaminant sources in the watershed
and potential exceedances of water quality standards.
Each sampling event will provide EPA scientists
opportunities to demonstrate sampling methods in the
field, train citizen researchers, and interact with
members of community.
Regional Laboratories provide the data to help
protect food sources and assess watershed quality

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Protecting Water: A Precious, Limited Resource
Water Quality Assessment and
TMDL Program Support
Crucial for management about our water resources,
water quality data are used to characterize waters,
identify trends, identify emerging problems, determine
whether pollution control programs are working, and
help direct pollution control efforts to where they are
most needed.
A TMDL is a calculation of the maximum amount of a
pollutant that a waterbody can receive and still meet
water quality standards, and an allocation of that load
among the various sources of that pollutant. Regional
Laboratories provide substantial analytical support for
water quality assessments to, and TMDL development
for, water bodies throughout the country.
Total Maximum Daily Load
(TMDL) Studies in Springfield,
Missouri
The Wilson, Jordan and Pearson Creek TMDLs were
established January 2011, after named as impaired
waters from multiple point sources and urban nonpoint
sources. The pollutant causing the impairment is listed
as unknown; however, toxicity from multiple pollutants
and changes in hydrology from increased impervious
surfaces are the suspected cause of the impairment.
By establishing these TMDLs, EPA met the milestones
of the 2001 Consent Decree. However, after a ruling in
2011, EPA was required to further consider impacts
based on additional water quality criteria. To meet
settlement agreement, Region 7 conducted monthly
water and sediment sampling and diurnal dissolved
oxygen monitoring. Water grab samples were analyzed
for total phosphorus, total nitrogen, suspended solids,
dissolved solids, total solids, dissolved metals, and
hardness. Sediment grab samples were analyzed for
metals and polycyclic aromatic hydrocarbons (PAHs).
Water and sediment samples from urban streams in
Springfield were collected for over 10 different
traditional methods, including metals, PAHs, anions,
hardness, semi-volatile organics, and pH. This effort
supports EPA's interest in determining what pollutants
may be present, and whether the creeks meet Missouri
water quality standards to effectively restore those
streams. The Region 7 laboratory and field staff
collected water and air samples and performed field
analysis using the mobile laboratory, while traditional
analysis was conducted by the Region 7 Laboratory.
The results will be used to confirm the conditions in the
Springfield urban streams for the TMDL Program.
All 10 Regional Laboratories provide scientific expertise to support Regional and National Water
Programs and Initiatives, which can include:
•	analysis
•	field support
•	quality assurance
•	data review
•	technical support

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Addressing Climate Change and Improving Air Quality
Protect and improve the air so it is healthy to breathe and risks to human health and the
environment are reduced. Reduce greenhouse gas intensity by enhancing partnerships
with businesses and other sectors and common-sense regulatory initiatives.
FY2015 HIGHLIGHTS
AIR QUALITY

INDOOR AIR




I
PM 2.5

/


Regional Laboratories actively support the
objectives of the Agency's air goals through a
variety of activities. These activities include:
•	technical support and training
•	air monitoring and air monitoring quality
assurance
•	laboratory support for various air toxics
assessments
•	laboratory support for numerous other local
projects that address specific community
risks and method development.
Fuel Oil Terminal Air Emissions
In the summer of 2015, the Region 6 Compliance Assurance and
Enforcement Division's Air/Toxics Enforcement Section conducted an
inspection at a fuel oil storage terminal located near the Houston, Texas
Ship Channel. The terminal's operations and associated processes are
subject to Clean Air Act (CAA) requirements, including the Texas State
Implementation Plan.
At the on-site inspection, Region 6 performed a number of activities to
evaluate the site's ambient air emissions using different instruments, including photoionization detectors (PIDs)
and special infrared (IR) cameras. Air samples were taken based on PID screening and IR results using
SUMMA™ canisters. The stainless steel evacuated canisters are widely used for sampling volatile organic
compounds (VOCs). Grab sampling using SUMMA™ canisters was representative of the ambient air at the
facility, because the samples were taken on the downwind side of the oil storage tanks for the No, 6 fuel oil, and
samples were taken in background air for comparison purposes.
Region 6 Laboratory provided analysis of the SUMMA™ air samples taken at
the fuel oil storage site. Extensive dilutions of many samples were required to
keep instrument saturation to a minimum. High concentrations of VOCs, and
hydrocarbons (HCs), were identified by the combined gas chromatography/
mass spectrometry (GC/MS) instrument used to analyze the canisters for HCs.
including benzene, toluene, ethylbenzene, and xylenes (BTEXs). BTEXs are
usually considered among the worst of the HC ambient air pollutants due to
associated breathing difficulties of long-term exposures and possible cancer
risks. Region 6 Laboratory sample results were provided to the Enforcement
Division and wiil aid future actions for this site.

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Addressing Climate Change and Improving Air Quality

Air Monitoring in Pennsylvania
CONSOL Energy Inc. and the Allegheny County
Pennsylvania Airport Authority unveiled a proposed
Marcellus Shale natural gas production project on
Pittsburgh International Airport (Imperial Pointe)
property. The proposed plan outlines six well-pad
locations and three centralized water impoundments.
Allegheny County Health Department (ACHD) Air
Quality Program, Monitoring section, will conduct
ambient air sampling for VOCs in the Imperial Pointe
residential neighborhood in Findlay Township. This air
monitoring program is part of a larger national network
designed by EPA to monitor ambient air pollution
concentrations nationwide.
The ACHD monitoring program is part of the State and
Local Agency Monitoring Station (SLAMS). SLAMS
consists of a network of monitoring stations whose size
and distribution is determined by the needs of state and
local air pollution control agencies, to meet their
respective state implementation plan (SIP)
requirements. The primary purpose of air monitoring is
to determine if concentrations of certain VOCs can be
shown to statistically correlate to gas exploration and
production activities. Air samples are being collected
for a period of up to 18 months, which began in April
2014 and will continue for a year after gas exploration
begins. The samples are being analyzed by Region 3
Laboratory air chemists.
682
PM 2.5 AUDITS CONDUCTED IN FY2015
Sorbent Tube Analysis of High
Humidity Samples
An increase in understanding the high concentrations of
trichloroethylene (TCE) in homes sitting above
contaminated groundwater plumes has caused the
agency to investigate the gas vapors under the homes
and the ground surrounding the homes. The sample
type is vapor intrusion and is accomplished by drilling
holes through the foundation and collecting air samples
using TO-15. air canisters. Because of the cost and
limited resources, sorbent tubes were being
investigated for these high-humidity air samples. If the
target list is limited to chlorinated VOCs, a single
sorbent tube using tenax can capture the sample and
analyze it for TCE. This method is anticipated to
produce reliable data. The sorbent was identified and
tests began in FY2015. The method should be finalized
in FY2016. Once this method is completed, this
technique could be used for testing air samples in caves
and other high-humidity situations.
PM 2.5 Performance
Evaluation Program (PEP)
The goal of the PEP is to evaluate total measurement
system bias of the particulate matter (PM) 2.5
monitoring network. The laboratory component of the
program includes PM filter handling, inspection,
equilibration, and weighing: data entry, validation,
management, and distribution to client regions; and filter
archival and data submittal to the Air Quality System
(AQS). The PM filter weighing laboratory is located at
Region 4. Other Regional Laboratories also provided
support for PEP through performance evaluation audits,
quality assurance collocations, and PEP audits.
Regional Laboratory staff provided training classes for
the PM 2.5 PEP program.


Regional Laboratories have the capability to analyze for Organic Compounds in Air








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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Taking Action on Toxics and Chemical Safety
Keeping communities safe and healthy by reducing risks associated with exposure to
chemicals in commerce, indoor and outdoor environments, and products and food.
FY2015 HIGHLIGHTS
DIESEL SPILL RESPONSE
PESTICIDES & ASBESTOS
BROWNFIELDS SUPPORT
Incremental Sampling Technique
Used at Oregon Brownfields Site
The Comprehensive Environmental Response,
Compensation, arid Liability Act (CERCLA), or
Superfund, and the Resource Conservation and
Recovery Act (RCRA) provide the legal basis for
EPA's efforts to preserve and restore land using
the most effective waste management and
cleanup methods available. By supporting these
programs, the Regional Laboratories:
•	Analyze hazardous and non-hazardous
waste
•	Implement applied research and method
development to meet evolving analytical
needs of Superfund and RCRA programs
•	Ensure vigilant readiness for emergency
response to environmental disasters,
hazardous materials releases, time-critical
removals, and inland oil spills.
The Heritage Square site is located in a primarily commercial
area of downtown Astoria, Oregon. Historical activities at
this site, including an automobile repair garage and paint
shop, dry cleaner, and printer are potential sources of
contamination that warranted further investigation. An
Environmental Site Assessment (ESA) also identified
indications of underground storage tanks.
To support site remediation, Region 10 Laboratory applied
the incremental sampling methodology (ISM) to process
samples from excavated soil piles. SM processing (or
homogenization procedure) consists of many small
increments of soil materials over a wide area and requires
the use of unique grinding and mixing equipment with
procedure-specific Quality Control (QC) samples (process
blanks). The ISM application results yield the need for fewer
samples to be analyzed and better statistical assessment of
soil contamination.
Region 10 Laboratory analyzed the processed soil samples
for polychlorinated biphenyls (RGBs), PAHs and petroleum
hydrocarbons. Site support is continuing through FY2016.

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Taking Action on Toxics and Chemical Safety
West Virginia Diesel Spill
A tractor trailer accident resulted in a spill of
approximately 4,000 gallons of diesel fuel in Greenbrier
County, West Virginia. Two drinking water intakes,
within 50 miles of the spill, were shut down. Region 3
Laboratory analyzed drinking water samples for semi-
volatile organic analysis (SVOA), polycyclic aromatic
hydrocarbons (PAHs) by selected ion monitoring (SIM),
and volatile organic analysis (VOA) under an expedited
turnaround time (less than 7 days).
Sumas Mountain Asbestos
Region 10 has been investigating the release of
asbestos from an earth-flow landslide on the west side
of the Sumas Mountain near Bellingham, Washington,
since 2006. The landslide initiated around 1940,
resulting in significant deposition of asbestos-bearing
sediments into Swift Creek and the Sumas River,
especially during periods of rain and snow melt.
Sampling and analysis conducted by EPA over the past
several years has confirmed relatively high
concentrations of chrysotile asbestos and lower
concentrations of actinolite asbestos in water and
sediments that settle in Swift Creek and the Sumas
River. Historically, when the deposition of sediments
impaired the flow of Swift Creek, it would flood onto
farmland and surrounding residential properties. The
sediments were dredged and placed along the banks of
the creek and offered to the community as
fill. Recognizing the potential health
risk to the community, EPA worked
with local government to restrict the
dredging of Swift Creek sediments.
However, during significant rain
events like what occurred in the winter of 2009, the
Sumas River flooded and deposited asbestos-bearing
sediments over a much larger area. Appropriate
disposal of these sediments is not feasible due to the
extraordinarily high costs associated with transport and
disposal. Over last year, Region 10 Laboratory
continued supporting assessment efforts at the Sumas
River site using a variety of analytical tools, including
polarized light microscopy (PLM), powder X-ray
diffraction (XRD), and scanning electron microscopy
(SEM) with energy-dispersive x-ray analysis (EDXA) to
determine the mineralogy of sediments with emphasis
on the identification and quantitation of asbestos.
In addition, an experimental device called a fluidized
bed asbestos segregator (FBAS) has been used to
process samples from the site. The FBAS uses air
elutriation to separate fibrous mineral structures from
heavier matrix particles and deposits them onto a filter
that is analyzed by transmission electron microscopy
(TEM). which is contracted out to commercial
laboratories. This unique approach allows
measurement of low-level concentrations of asbestos
structures that may become airborne when soil and
sediments are disturbed. The sensitivity of this
technique far exceeds traditional methods, such as PLM
and XRD.
Over 6,000 analyses conducted by Regional
Laboratories for Emergency Response Activities

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Taking Action on Toxics and Chemical Safety
Village Creek Dieldrin Study
Achieves Lower Detection
Limits to Prevent Health
Impacts
Village Creek has been classified as impaired on the
Alabama Department of Environmental Management
(ADEM) 303(d) list for the legacy pesticide dieldrin,
Dieldrin is an organochlorine pesticide and is no longer
produced in the U.S. due to harmful bioaccumulative
effects on humans, fish, and wildlife.
Previous ADEM data collected showed no detection of
dieldrin, leading to consideration of removal of Village
Creek from 303(d). However, the reporting level of
ADEM data was above the 0.03 ng/l human health
criteria making an impairment decision uncertain.
By using modified methods and cutting-edge analytical
technology, Region 4 Laboratory was able to achieve
detection limits almost 300 times lower than the routine
detection limits previously achieved by the State where
dieldrin was not detected. Results of the analysis
showed concentrations of dieldrin in Village Creek
roughly 40 times higher than the human health criteria.
The State of Alabama reconsidered delisting and is
planning further stream assessments. Had delisting
occurred, efforts to remediate the stream segment
would have ceased and undetected amounts of dieldrin
remaining in the stream could biomagnify through the
food chain and contribute to severe health effects.
	~
mm r * m
58% of Regional Laboratory analyses support
Superfund Program
Trichloroethylene (TCE)
Investigated at Meramec
Caverns
The topography of Southeastern Missouri is
characterized by numerous caves, sinkholes, fissures,
and underground streams. Meramec Caverns is one of
the largest cave systems in Missouri. Unfortunately, it is
also impacted by a subsurface plume of TCE that
originates from a site approximately 5 miles away.
Concerns were raised about TCE exposures to visitors
and employees touring or working in the
commercialized portion of Meramec Caverns and the
ecological health of cave dwelling fauna.
Using Region 7 mobile laboratory, on-site TCE
concentrations in both the commercialized and native
portions of Meramec Caverns were analyzed by GC/MS
on samples of cave air using portable bag samplers.
The data generated characterized distribution,
concentration, and ventilation of TCE throughout the
cave complex. This is a multi-year project and
continues into FY2016.
Pesticide Residue Tested
The Office of Pesticide Programs' (OPP) Environmental
Fate and Effects Division (EFED) requested assistance
from the Region 3 Laboratory for total organic carbon
(TOC) analysis, which was part of a study on pesticide
residues. OPP analyzed 50 pesticide soil samples and
needed TOC results to use in risk assessment
calculations for bioavailability. Region 3 Laboratory was
able to analyze all 50 samples within a very short
timeframe.

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Making a Visible Difference in Communities across the Country
Align community-based activities to provide seamless assistance to communities, both urban
and rural, while maximizing efficiency and results. Expand support of community efforts to
build healthy, sustainable, green neighborhoods and reduce and prevent harmful exposures
and health risks to children and underserved, overburdened communities.
FY2015 HIGHLIGHTS
LEAD IN SCHOOLS
COMMUNITY SUPPORT
HEALTHY GARDENS
Environmental and public health impacts affect people
most significantly where they live - at the community
level. EPA is focused on providing better support to
communities, especially in environmentally-
overburdened, underserved, and economically-
distressed areas where the needs are greatest.
Regional Laboratories coordinate technical assistance
and other resources across EPA Programs; with
states, tribes, and local governments; and with other
federal agencies to support communities as they
pursue environmental improvements that enhance
economic opportunity and quality of life.
Assessing Soil Quality Impacts to Gardening
Soil safety is of particular interest to Austin, Texas and its citizens due to the city's food-to-table ordinance allowing
any garden to provide food to local restaurants. Garden soils
may harbor contaminants, such as heavy metals and organic
chemicals. Because of Austin's food-to-table ordinance, Austin's
Brownfields Revitalization Program requested assistance from
Region 6 in conducting a Soil Kitchen, an event that provides
home, school, and community gardeners with the opportunity to
test garden soil samples. Since soil safety testing can be quite
costly, free testing is a valuable service.
Region 6 collaborated with Austin's Brownfields Revitalization
Program to provide testing of garden samples during the Soil
Kitchen. Region 6 Laboratory deployed its mobile lab and
provided on-site X-ray fluorescence (XRF) testing of soil samples
for the presence of heavy metals during the event. Additional garden
samples were analyzed for legacy pesticides and semi-volatile
compounds.
Region 6 Laboratory, Houston, analyzed over 150 garden soil
samples.
There were no significant amount of metals; small amounts of lead and
arsenic were present in some soil samples. Gardeners were provided
test results through a private online process, along with best and safe gardening practices and recommendations for
gardening in contaminated soils. The Soil Kitchen raised awareness about soil health and safety.
9
18
EPA-910-R-17-001

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Making a Visible Difference in Communities across the Country
Lead in Schools Project
Lead in drinking water can have adverse health effects, especially
for children who can experience impaired mental development, IQ
deficits, shorter attention span, and lower birth weight. Testing for
lead in schools and daycare is important since children spend a
large portion of their day in these types of facilities. The 1988 Lead
Contamination Control Act (LCCA) is aimed at identifying arid
reducing lead in drinking water in schools and child care facilities.
In 2013, the Office of Water (OW) established a 3-year pilot study to
promote awareness of the potential sources of lead in schools and
child care facilities. The primary objective is to reduce children's
exposure to lead from drinking water. The pilot study was in
collaboration with the Kellogg Foundation and the Calhoun County Public Health Department located in Calhoun
County, Michigan. The OW pilot study was a first step in a nationwide effort to promote awareness of the potential
exposure to lead in schools and day care facilities with a focus on testing and remediation options.
Based on its active involvement in the Lead-in-Schools Program, OW reached out to Region 2 for analytical support
for this study. Due to the large-scale nature of this study, Region 2 Laboratory coordinated the analytical support for
this study, using RLN resources. Regions 2, 3, 5, 6, 7, 9, and 10 Laboratories participated in the study, providing
over 4,000 analyses of over 100 schools and child care facilities in Calhoun County, Michigan, over a 3-year period
between 2013 and 2015. The use of regional laboratories yielded significant cost savings compared to use of
commercial laboratories, allowing precious funds to go toward the sampling of many more schools and day care
facilities. The analytical results and field data were directly used to make a determination as to whether drinking
water distributed from outlets were contaminated with lead above the 20 ug/L standard and, where applicable,
provided guidance to the school/facility on how to remediate.
The Kellogg Foundation, the grantee for this project, plans to publish a guide that synthesizes lessons learned and
best practices regarding lead sampling and analysis in schools and childcare facilities across the country. The aim of
this guide is to help staff and other stakeholders (for example, parents and students) from schools and childcare
facilities develop their own affordable, voluntary program. In addition, other groups (for example, public health
partners, community members, and researchers) may choose to use this as a guide to support the implementation
and evaluation of these efforts among schools and childcare facilities in their area.
Former Kil-Tone Superfund Site
Region 2 Laboratory provided analytical support to the former Kil-Tone Superfund site. The Former Kil-Tone
Company Site is located within the City of Vineland, Cumberland County, New Jersey. As part of a two-phase,
extensive evaluation to assess contamination found in residential and surrounding areas, including flood plains, the
Region 2 Laboratory analyzed over 2,000 soil samples, primarily for metals analysis, in support of removal and
remedial program activities at the site. The results were used to calculate the Hazard Ranking System (HRS) score
and propose that the former Kil-Tone Superfund Site be listed on the National Priorities List (NPL).
The laboratory provided validated results within 4 weeks of each sample delivery. Internal staff performed these
tasks, which resulted in an overall cost savings, keeping site cleanup activities on track.

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Launching a New Era of State, Tribal, and Local Partnerships
I
Strengthen partnerships with states, tribes, local governments, and global communities
central to the success of the national environmental protection program through consultation,
collaboration, and shared accountability.
\
}
FY2015 HIGHLIGHTS
CARIBBEAN SCIENCE CONSORTIUM

i

MUCKLESHOOT TRIBE

!
J
/
STATE AND TRIBE SUPPORT
I
J
By providing support at the regional level,
opportunities abound to work in concert with
states, tribes, and local entities providing
technical support. Types of activities where
Regional Laboratories become involved
include:
•	Analytical support to states or tribes
•	Assisting communities and volunteer
monitoring groups with implementation of
Citizen Science
•	Providing training and technical support,
including training in preparation of the
Quality Assurance Project Plans (QAPPs).
Caribbean Science Consortium
A common problem in the Caribbean is that individual
territories and academic research institutions lack
adequate resources (in terms of capability or capacity) to
conduct environmental science programs in any
systematic, comprehensive way.
Representatives from Region 2 have addressed this
issue by establishing a collaborative partnership with
government and academic scientific institutions in the
U.S. Virgin Islands and Puerto Rico: EPA Region 2
Caribbean Science Consortium. The Consortium
comprises members of Region 2, government and university organizations (including minority academic institutions)
in Puerto Rico and the U.S. Virgin islands and is managed by Region 2 Laboratory.
The Consortium's main objective is to expand science collaboration on mutual environmental science programs,
including the leveraging of resources, technical assistance, education, and outreach. The Consortium established a
Charter and an annual work plan. The Consortium focused on addressing "signature" research issues on the islands,
including water quality of rural communities not served by public dr inking water.
	I
EPA-910-R-17-001
20

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U.S. EPA Regional Laboratory System	FY2015 Annual Report


Launching a New Era of State, Tribal, and Local Partnerships

Region 10 Laboratory supported a Regional Applied
Research Effort (RARE) study conducted along the
LDW that may help predict the fate of inorganic arsenic
in Mya. Mya samples collected from the LDW
experimental area were analyzed for arsenic species
using a method developed at Region 10 Laboratory that
uses liquid chromatography (LC) and inductively
coupled plasma/mass spectrometry (ICP/MS). Region
10 Laboratory also developed the use and analysis
method capability of diffusive thin-film gradient (DGT)
samplers that were included in the study. The DGT
sampler technology was studied to determine if it was
as a suitable substitute for actual Mya in exposure
studies. This work is continuing into FY2016.
Lower Duwamish Waterway
Arsenic Study
Region 10 Laboratory developed a method for
detecting arsenic speciation along with assessing the
effectiveness of a new sampling technology for the
Lower Duwamish Waterway (LDW) Superfund Site.
This site is a 5 -mile stretch of the Duwamish River
that flows into Elliott Bay in Seattle, Washington. A
century of heavy industrial use has left the waterway
contaminated with toxic chemicals from many
sources - industries along its banks, storm water
pipes, and runoff from upland activities, streets, and
roads. Pollution in the river sediments includes
PCBs, dioxins/furans, carcinogenic PAHs, and
arsenic. Many of these chemicals are persistent in
the environment and tissue concentrations are
unsafe in some resident fish and shellfish.
The LDW is part of the Muckleshoot Tribe's Usual
and Accustomed tribal fishing areas. The
Muckleshoot Tribe expressed interest in harvesting
clams in the waterway, but currently does not
because of chemical contamination. Consumption of
inorganic arsenic in clams, chiefly Mya arenaria
(Mya), contributes 40-50% of the total risk in the
Reasonable Maximum Exposure (RME) seafood
consumption scenario for tribal fishers. Understanding
how and from which media Mya bioaccumulates arsenic
is critical to make science-based decisions on whether
and how to remediate arsenic-contaminated sediments
so that clam consumption-based risks to Tribal
members is minimized. Although, reducing sediment
arsenic concentrations is expected to decrease
inorganic arsenic (the most toxic form) concentrations in
tissues, it is not currently possible to reliably predict this
decrease. The relationship between sediment and
Mya tissue concentrations has considerable variability.
Mya, as a filter feeder, likely consumes suspended
solids-associated arsenic (seston) and the importance
of this pathway has not been quantified. Mya retains
approximately 50% of bioaccumulated arsenic in
inorganic form rather than transforming it to less toxic
organometallic form, as occurs in other clam species.
EPA-910-R-17-001
21

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Embracing EPA as a High-Performing Organization
Maintain and attract EPA's diverse and engaged workforce of the future with a more
collaborative work environment. Modernize our business practices, taking advantage of
new tools and technologies, and improve the way we work as a high-performing Agency.
FY2015 HIGHLIGHTS
QA & FIELD ACTIVITIES
CROSS-AGENCY COLLABORATION
I
LEAN GOVERNMENT
One of the most important regional and state
laboratory partnerships is sharing unique expertise,
when needed. In 2015, Regional Laboratories
supported various projects. Laboratories relied on the
expertise of other Regional Laboratories with unique
capability/capacity.
Collaboration with the Office of Research and
Development (ORD) provided opportunities for
scientists to expand their knowledge and skills through
the Regional Research and Partnership Program.
Quality Assurance Field Activities
Procedures (formerly FOG)
Under the leadership of Region 4, the Field Operations Group
(FOG) develop operational guidelines for managing and ensuring
adequate quality of the Agency's field activities.
FOG's main goals:
•	Promote greater consistency in field measurement and sampling activities that are performed by EPA
•	Produce reliable and legally-defensible data from field measurement and sampling activities
•	Participate in training of other regions and programs to assure the implementation of the program

In 2013, by direction from EPA's Acting Deputy Administrator, ali EPA organizations conducting field activities were
required to implement the FOG Guidelines by February 2016. The Office of Environmental Information (OEI), with
the help of Region 4 personnel, authored the EPA QA Field Activities Procedure (QAFAP), which incorporates all 10
FOG Guidelines, adopted in September of 2014 by OEI as QAFAP (CIO 2105-P-02.0) under the Agency's Quality
Policy. To facilitate the Agency's implementation of the QAFAP, EPA Senior Leadership organized the FOG
implementation Team (FIT) with Region 4 having a pivotal role as national leaders. Region 4 provided seven
members to FIT, including four QAFAP subject matter experts (SMEs) and the leadership of two high-level
managers and the Deputy Regional Administrator as a champion to support this national initiative.
Region 4 SMEs developed and provided training on QAFAP requirements across the Agency, drafted procedural
templates for other EPA organizations to use, as needed, and conducted gap assessments at ten regions and five
Offices to assess the Agency's progress toward implementing the QAFAP by February 2016. The Region 4 FIT
management team provided the necessary leadership across the Agency that has made this initiative so successful.

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Embracing EPA as a High-Performing Organization

Collaborating within Regional
Lab Network
Region 10 provided specialized incremental sampling
methodology (ISM) to assist Region 3 on a Superfund
Site.
The Clearview Landfill Superfund Site, located near
Philadelphia, PA, was privately owned and operated
without a permit from the 1950s to 1970s, and was used
for the disposal of municipal and industrial waste
collected from the City of Philadelphia and portions of
Delaware County. In 1973, the owners were ordered to
cease ail waste disposal activities at the landfill and
follow a prescribed closure plan, but the property
continued to be used for other waste disposal
operations for many years after the cease order.
EPA conducted a Human Health Risk Assessment
(HHRA) to estimate the risks from the presence of
contamination, which included polychlorinated PCBs,
PAHs, and heavy metals. The outcome of HHRA
indicated unacceptable risks related to soils (for
applicable areas and receptors), shallow groundwater,
and modeled fish tissue concentrations that exceeded
EPA target levels.
In partnership with Region 3, Region 10 Laboratory
supported a remediation project for the site by preparing
samples collected using the incremental sampling
methodology (ISM). This ISM processing (or
homogenization procedure) of soil materials consists of
many small increments over a wide area, and requires
the use of unique grinding and mixing equipment with
procedure-specific Quality Control (QC) samples
(process blanks). The ISM application yields fewer
samples requiring analysis and better statistical
assessment of soil contamination.
ORD Collaboration using
RARE
The Regional Applied Research Effort (RARE) is an
Office of Research and Development (ORD) program
that responds to high-priority research needs of EPA
Regions. Region 3 identified a research need to
investigate the impact of highly brominated source
water into drinking water treatment plants. The
"RARE Bromide" project was a multi-year project
conducted by the Region 3 Water Protection Division
(WPD). WPD looked at the effect of bromide on the
formation of brominated trihaiomethanes (THMs) in the
surface-water intake at public water system treatment
plants. Region 3 Laboratory provided support by
analyzing thousands of samples for total
trihaiomethanes (TTHMs), alkalinity, ammonia, pH. and
conductivity. RARE Bromide research is ongoing.
Region 10 Laboratory analyzed the processed soil
samples for PCBs and PAHs. The analysis results were
used to develop final design plans and specifications for
the remedial action phase of the project. This support is
ongoing through FY2016.
Regional Laboratories routinely conduct studies
under the RARE program sponsored by Office of
Research and Development (ORD) with
152
projects in 2015
I
EPA-910-R-17-001
23

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Embracing EPA as a High-Performing Organization
Laboratory LEAN Events
Regional Laboratories use LEAN to improve processes
and eliminate unnecessary steps. One example is the
outcome of the Region 7 Laboratory LEAN events that
began in FY2015 with a goal to make routine laboratory
processes more efficient. Two events were held in
FY2015 with more events scheduling in FY2016.
The first FY2015 event dealt with a problem related to
completion of documentation for Demonstrations of
Capability (DOCs) in Laboratory Information
Management System (LIMS). Historically,
documentation showing the completion of DOCs took 3-
6 months after completion of the analysis. Even though
the DOC packages were similar to actual data
packages, the DOC packages were not seen as a
priority. A team of chemists, quality staff, and computer
staff met for two 4-hour sessions for a mini-Kaizen
event. At these sessions, the team learned that a 5-day
turnaround (comparable to real data) was possible. The
team identified and eliminated wasted steps and
duplicate steps. Ultimately, the team recommended a
routine turnaround of 10 working days as the goal for
streamlining the process and recommended revisiting
the process in FY2017.
The second FY2015 event addressed a problem with
sample cubitainers. The manufacturer of cubitainers
sold its business and the production was moved to
China. Obtaining shipments of cubitainers in a timely
manner, and receiving properly-cleaned cubitainers
became a significant challenge. A team, including
purchasing, sample receipt, inspectors, field staff,
disposal staff, and a facilitator met and discussed the
history and problems. They mapped the major steps
and methods of the process that used the cubitainers.
Specifications of the substitute bottle that would work at
all stages of the sample collection and analysis were
identified. Finally, catalog options of possible
substitutes and next steps to ensure that new bottles
would perform well and meet laboratory specifications
were reviewed. After the meeting, the solution was
identified and implemented immediately. The
unexpected benefit of this event was a cost savings of
$30,000 a year, using the substitute, pre-cleaned bottle.
LEAN-a set of principles and methods used to
identify and eliminate waste in any process, helps
organizations improve speed and quality of their
processes. Kaizen events are used to map out the
process and find ways to streamline or eliminate
unnecessary
steps.
Region 7 Laboratory used
Lean Process yielding
$30,000
a year cost savings
Efficacy of Disinfection —
Support to Office of Pesticides
and Pollutants
The Office of Pesticide Programs-Antimicrobial Division
(OPP-AD) recently requested assistance from the
Region 3 Laboratory in analyzing one of their
disinfectant products for silver content. The mission of
OPP-AD is to confirm, by analysis, the active
ingredients in current disinfectant products. This
confirmation is normally run using organic
methodologies. But in this case, silver was the active
ingredient and OPP does not have any metals
instrumentation.
Following the supplied manufacturer method (with minor
changes) Region 3 analyzed the disinfectant for silver.
Matrix issues caused the silver to fall out of solution;
therefore, the manufacturer's stated formulation did not
meet required specifications.

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Working toward a Sustainable Future

Advance sustainable environmental outcomes and optimize economic and social outcomes through
Agency decisions and actions, which include expanding conversation on environmentalism and
engaging a broad range of stakeholders.
\
FY2015 HIGHLIGHTS
Laboratory Enterprise Forum
Established
\
i
IMPROVING ANALYTICAL METHODS
EPA established the Laboratory Enterprise Forum (LEF)
in July 2015 to increase efficiency and impact of Agency


laboratory functions. The primary purpose is to
1
LABORATORY ENTERPRISE FORUM
strengthen communication, coordination, and
collaboration among the laboratory enterprise;
strengthen efficient and effective management
processes and strengthen overall interactions with
external lab organizations.


g
REDUCING CHEMICAL USE IN LABS
R
J
Solid-Phase Extraction (SPE)
Method Development and Partnership
Region 7 chemists have been developing several SPE
extraction methods, which reduce the solvent usage
while maintaining or improving detection limits. This year
is the culmination of extensive method development in
partnership with two different vendors and the American
Council of Independent Laboratories (ACIL). EPA
Region 7 was one of 25 laboratories participating in a
two-phase study that provided a technical response to
the comment period for the Code of Federal Regulations
(CFR) update of EPA Methods 608 and 625.
I
Perfluorinated compounds
(PFCs)
Region 5 Laboratory completed development of two
methods for the determination of Perfluorinated
chemicals (PFCs). The method for non-potable waters
was adopted by the American Society for Testing and
Materials (ASTM) as Method D7979 and the method for
soils and biosolids was adopted as D7968 in FY2015.
Both methods are being used to analyze samples by
Region 5 for the Office of Research and Development
(ORD), Cincinnati National Risk Management Research
Laboratory (NRMRL) study of PFCs from sewage plant
operations and land application of biosolids.
Regional Laboratories continue to explore
opportunities to reduce chemical usage or
improve processes.
I

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U.S. EPA Regional Laboratory System
FY2015 Annual Report

Section III — FY2015 Laboratory Accomplishment Results Summary
I
vvEPA
United States
Environmental Protection
Agoncy
i3A-i 0 R-17- 0

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
FY2015 Laboratory Accomplishment Results Summary
This section summarizes a number of the
common support services provided by the
RLN.
Because of the unique nature of the
support provided by Regional Laboratories,
the ideal Regional Laboratory scientist is
one part research scientist and one part
production scientist. Regional Laboratory
scientists are capable of developing
methods (often with short lead times),
focusing on quality control, and operating
under demanding delivery schedules.
Regional Laboratory staff support diverse
and challenging requests. During FY2015,
RLN supported more than 150,000
analyses. The distribution of work by the
RLN is shown in Figures 1 and 2. These
totals exclude Quality Control (QC)
samples, which add an additional 20%.
In keeping with prior years, the
Superfund program continues to
be the largest volume requestor of
analytical services (55.9%),
followed by Water Programs
(30.3%). Emergency Response
program support continues to be
significant at 3.7%, with RLN
laboratories analyzing 5,538
samples in conjunction with time-
critical responses to environmental
disasters, hazardous materials
releases, priority contaminant
removals, and other threats to
human health and/or the
environment, which aided in timely
and cost-effective decision-making
in the field. All 10 Regional
Laboratories augmented the
National Enforcement
Investigations Center's (NEIC's)
capacity in support of important
criminal cases, analyzing 196
criminal samples during the year.
Figure 1. Analytical Support to EPA Programs in FY2015
(150,587 Total Analyses)
Pesticides
Emergency
Response
<3.7%
TSCA
.<1%
LUST
<1%
Brownfields
<1%
RCRA
<1%_
Other
7.2%
Superfund
55.9%
Water
30.3%
Figure 2. FY2015 U.S. EPA Regional Laboratories Analyses Provided by Program
(150,572 Total Analyses)
90000
84133

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
FY2015 Laboratory Accomplishment Results Summary
Figure 3 summarizes the number of analytical projects supported by RLN according to EPA program element.
Collectively, RLN supported 1,753 projects.
Figure 3. Projects/Sites Supported by Regional Laboratory Data FY2015 by Program Element
(1,753 Total Sites/Projects Supported)
Superfund - Pre-remedial/Remedial	i	—	750
Field Sampling (field sampling audits and events, etc.)	< 235
Water-Enforcement	•	:—< 145
Water- Program Implementation (REMAP, TMDL, TOXNET. etc.)	i ¦ I 95
Superfund-Removal I fit
Water - Drinking Water Compliance and Emergencies I 78
Air- Program Implementation (monitoring, peimrts, etc.)	i < 74
Other	i	A 72
Superfund - Emergency Response
U—-j|
RCRA - Corrective Action
-4 34
RCRA - Enforcement
-1 24
LUST |
M 23
Brownfields \
-4 22
Pesticides |
-4 22
Criminal Investigation
U 15
TSCA - Enforcement
1 5
Air-Enforcement l
1 3
TSCA- Remedial I
1 3
A significant amount of
work supported during
the year required
methods be developed
specifically to address
the unique needs of a
particular region
(Figure 4). Often,
methods developed by
a region to address a
local environmental
challenge are
mobilized in other
regions as their benefit
is realized and/or as
the need arises.
Figure 4. Method Development Project Support to EPA Programs in FY2015
(93 Methods)
I
RCRA
Other
Superfund
24.7%
Water
43.0%
Emergency
Response
<1%
Pesticides
6.5%
Enforcement
3.2%
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U.S. EPA Regional Laboratory System
FY2015 Annual Report
L
Section IV — Regional Laboratories Capabilities Tables — FY2015
United States
Environmental Protection
Agoncy
SEPA
EPA-910-R-17-001

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Core Capabilities — FY2015
I. Chemistry
Analyte/Group Name
Sample Media
Analytical Technique
Regional Capability
INORGANIC CHEMISTRY
1
2
3
4
5
6
7
8
9
10
Acidity
Water
Titrimetric

X
X
X
X

X
X


Alkalinity
Water
Titrimetric
X
X
X
X
X
X
X
X
X
X
Asbestos
Solids/Bulk material
PLM
X





X
X

X
Soil/Sediment
PLM
X





X
X

X
Anions
Water
IC
X
X
X
X
X
X
X
X
X
X
Water
Titrimetric

X
X







Chromium, Hexavalent (Cr+6)
Water
Colorimetric

X

X


X


X
Soil/Sediment
Colorimetric



X





X
Water
IC


X
X
X
X
X

X

Soil/Sediment
IC


X

X





Cyanide, Amenable
Water
Colorimetric
X
X

X
X

X
X
X
X
Soil/Sediment
Colorimetric
X
X

X


X
X

X
Cyanide, Total
Water
Colorimetric
X
X
X
X
X
X
X
X
X
X
Soil/Sediment
Colorimetric
X
X
X
X
X
X
X
X

X
Waste
Colorimetric
X
X
X
X
X
X

X

X
Fluoride
Water
ISE
X
X

X
X





Water
IC
X
X
X
X
X
X
X
X
X
X
Hardness
Water
Colorimetric










Water
Titrimetric

X
X


X


X

Water
I CP/Calculation
X
X
X
X
X
X
X
X
X
X
Mercury, Total
Water
CVAA
X
X
X
X
X
X

X
X
X
Water
Direct Hg Analysis






X



Soil/Sediment
CVAA
X
X
X
X
X
X

X
X
X
Soil/Sediment
Direct Hg Analysis
X



X

X

X

Tissue (fish &/or plant)
CVAA
X
X
X
X
X
X

X
X
X
Tissue (fish &/or plant)
Direct Hg Analysis






X



Waste (oil, drum, etc.)
CVAA

X
X
X
X
X

X
X
X
Waste (oil, drum,(etc.)
Direct Hg Analysis



X

X




Mercury (TCLP)
Soil/Waste (oil, drum,
etc..)
CVAA

X
X
X
X
X

X
X
X
Soil/Waste (oil, drum,
etc..)
Direct Hg Analysis





X

X


Metals, Total
Water
ICP/AES
X
X
X
X
X
X
X
X
X
X
Soil/Sediment
ICP/AES
X
X
X
X
X
X
X
X
X
X
Tissue (fish &/or plant)
ICP/AES
X
X
X
X


X
X
X
X
Waste (oil, drum, etc.)
ICP/AES

X
X
X
X
X
X
X
X
X
Metals (TCLP)
Soil/Waste (oil, drum,
etc..)
ICP/AES

X
X
X
X
X
X
X
X
X
Metals, Total
Water
GFAA
X









Soil/Sediment
GFAA
X









Tissue (Fish &/or
plant)
GFAA
X









Waste (oil, drum, etc..)
GFAA
X









Metals (TCLP)
Soil/Waste (oil, drum,
etc..)
GFAA










Metals, Total
Water
I CP/MS
X
X
X
X
X
X
X
X
X
X
Soil/Sediment
I CP/MS
X
X
X
X
X
X
X
X
X
X
Tissue (Fish &/or
plant)
I CP/MS

X
X
X


X
X
X
X
Waste (oil, drum, etc..)
I CP/MS


X
X

X
X
X


Metals (TCLP)
Soil/Waste (oil, drum,
etc..)
I CP/MS



X

X
X
X

X
Nitrogen (Ammonia)
Water
Colorimetric

X
X
X
X
X
X
X
X
X
Soil/Sediment
Colorimetric


X
X
X

X



Water
Electrode

X








32
EPA-910-R-17-001

-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Core Capabilities — FY2015
I. Chemistry
Analyte/Group Name
Sample Media
Analytical Technique
Regional Capability
INORGANIC CHEMISTRY
1
2
3
4
5
6
7
8
9
10
Nitrogen (N03&/or N02)
Water
Colorimetric

X
X
X
X
X
X
X
X
X
Soil
Colorimetric



X
X

X


X
Water
IC
X
X
X
X
X

X
X
X
X
Soil
IC
X

X
X
X

X

X
X
Nitrogen, Total Kjeldahl
Water
Colorimetric

X
X
X
X
X
X

X
X
Soil
Colorimetric


X
X
X
X
X



Perchlorate
Water
IC




X

X

X

Soil
IC






X

X

Water
IC with LC/MS confirmation


X

X




X
Water, Soil/Sediment
LC/MS


X






X
Water
LC/MS/MS
X




X

X
X

Phosphorus, Ortho
Water
Colorimetric
X
X

X

X

X

X
Water
IC
X
X
X
X
X

X
X
X
X
Phosphorus, Total
Water
Colorimetric
X
X
X
X
X
X
X
X
X
X
Soil
Colorimetric
X

X
X
X




X
Sulfate
Water
IC
X
X
X
X
X
X
X
X
X
X
Soil
IC
X

X
X
X

X
X
X

Water
Turbidimetric
X
X








Soil
Turbidimetric
X









Sulfide
Water
Colorimetric

X




X



Soil
Colorimetric










Water
IC, Turbidimetric





X




Soil
Titrimetric

X






X

ORGANIC CHEMISTRY
BNA
Water
GC/MS
X
X
X
X
X
X
X
X
X
X
Soil/Sediment
GC/MS
X
X
X
X
X
X
X
X
X
X
Waste (oil, drum, etc..)
GC/MS
X
X
X
X
X
X
X
X
X
X
Tissue (fish &/or plant)
GC/MS



X





X
BNA (TCLP)
Solid/Waste
GC/MS

X
X
X
X
X
X
X
X
X
BNA (TPH)
Water
GC/MS or GC


X
X
X
X
X
X
X
X
Soil/Sediment
GC/MS or GC


X
X
X
X
X
X
X
X
BOD
Water
Membrane Electrode

X
X
X
X
X
X
X
X
X
COD
Water
Photometric


X


X




Water
Colorimetric

X
X

X

X
X


EDB& DBCP
Water
GC/ECD
X


X
X
X

X
X

Herbicides
Water
GC/ECD
GC/NPD



X

X
X



Soil/Sediment
GC/ECD
GC/NPD





X
X



Waste (oil, drum, etc..)
GC/ECD
GC/NPD






X



Tissue (fish &/or plant)
GC/ECD
GC/NPD






X



Herbicides (TCLP)
Solid/Waste
GC/ECD



X

X
X



Solid/Waste
HPLC/UV Detection


X







Oil & Grease
Water
Gravimetric

X
X
X
X

X


X
Soil/Sediment
Gravimetric

X




X
X


Pesticides/PCBs
Water
GC/ECD
X
X
X
X
X
X
X
X
X
X
Soil/Sediment
GC/ECD
X
X
X
X
X
X
X
X
X
X
Waste (oil, drum, etc..)
GC/ECD
X
X
X
X
X
X
X
X
X
X
Tissue (fish &/or plant)
GC/ECD
X
X

X


X
X

X
Pesticides (TCLP)
Solid/Waste
GC/ECD

X
X
X
X
X
X
X
X

Phenolics
Water
Gravimetric

X
X



X
X


Soil/Sediment
Gravimetric


X



X
X


I

-------
I
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Core Capabilities — FY2015
I. Chemistry
Analyte/Group Name
Sample Media
Analytical Technique
Regional Capability
ORGANIC CHEMISTRY
1
2
3
4
5
6
7
8
9
10
PAHs
Water
GC/MS
X
X
X
X
X
X
X
X
X
X
Soil/Sediment
GC/MS
X
X
X
X
X
X
X
X
X
X
Air
GC/MS
X





X



Tissue (fish &/or plant)
GC/MS
X


X


X


X
Waste (oil, drum, etc..)
GC/MS
X
X
X
X

X
X
X

X
TOC
Water
Combustion/IR

X
X
X
X

X
X

X
Soil
Combustion/IR

X
X
X
X

X
X

X
Water
UV/Persulfate


X


X

X
X

VOA
Water
GC/MS
X
X
X
X
X
X
X
X
X
X
Soil/Sediment
GC/MS
X
X
X
X
X
X
X
X
X
X
Air
GC/MS
X

X
X
X
X
X
X
X

Waste (oil, drum, etc..)
GC/MS
X
X
X
X

X
X
X
X
X
Water
GC



X



X


Soil/Sediment
GC



X



X


Waste (oil, drum, etc..)
GC

X

X
X
X
X
X

X
VOA (TPH)
Water
GC/MS or GC



X
X
X
X
X
X
X
Soil/Sediment
GC/MS or GC



X
X
X
X
X
X
X













II. Physical and Other Determinations
Flash Point
Aqueous/Liquid Waste
(oil, drum, etc..)
Pensky-Martens or
Setaflash
X
X
X
X
X
X
X


X
Conductivity
Water
Specific Conductance
X
X
X
X
X
X
X
X
X
X
Ignitability
Soil/Sediment
Ignitability of Solids

X
X
X
X
X
X
X

X
Waste (oil, drum, etc..)
Pensky-Martens or
Setaflash Closed Cup

X
X
X
X
X
X
X

X
pH
Water
Electrometric
X
X
X
X
X
X
X
X
X
X
Soil/Sediment
Electrometric
X
X
X
X
X
X
X
X
X
X
Waste (oil, drum, etc..)
Electrometric
X
X
X
X
X
X
X
X
X
X
Solids, Non-Filterable
Water
Gravimetric
X
X
X
X
X
X
X
X
X
X
Solids, Percent
Soil/Sediment
Gravimetric
X
X
X
X
X
X
X
X
X
X
Solids, Total
Water
Gravimetric
X
X
X
X
X
X
X
X
X
X
Solids, Total Dissolved
Water
Gravimetric
X
X
X
X
X
X
X
X
X
X
Solids, Total Volatile
Water
Gravimetric



X
X
X
X
X
X
X
Turbidity
Water
Nephelometric
X
X
X
X
X
X
X
X
X
X













III. Biology/Microbiology
Coliform, Total
Water, Soil &/or
Sludge
Various
X
X
X


X
X
X
X
X
Coliform, Fecal
Water, Soil &/or
Sludge
Various
X
X
X


X
X
X
X
X
E. coli
Water, Soil &/or
Sludge
Various
X
X
X


X
X
X
X
X
Toxicity (Acute & Chronic)
Water
Fathead, Ceriodaphnia
X

X


X

X


Heterotrophic PC
Water
Various
X
X
X


X
X
X
X
X
34
EPA-910-R-17-001


-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Unique Capabilities — FY2015
REGION 1
Analyte/Group Name
Sample Media
Analytical Technique
Supported Program(s)
Comments
INORGANIC CHEMISTRY
Inorganic Anions
Water
IC (EPA Method 300.0)
Water

Mercury
Water, Tissue
Direct Mercury Analyzer
(Thermal Decomposition,
Amalgamation & Atomic
Absorption
Spectrophotometry)
EPA Method 7473
Superfund, Water

Metals
Water, Sediment, Soil,
Waste (drum), Paint,
Dust, Cosmetics
XRF (EPA Method 6200)
Superfund, TSCA (Pb)
Field Screening and
Laboratory Testing
Perchlorate
Water
LC/MS/MS (EPA Method
331.0)
' Superfund/Water






ORGANIC CHEMISTRY
Carbonyls
Air
HPLC (EPA Method TO-11A)
Air

1,4-Dioxane
Water
GC/MS Purge & Trap (EPA
Method 8260)
Superfund

Ethylene Glycol
Water
GC


Explosives
Water, Soil
HPLC (EPA Method 8330)
Superfund

Oil Identification
Water
GC/FID (ASTM D-3415-79)
Superfund

Organic Compounds
Solid, Liquid
FTIR
Superfund - ERB
Unknown ID
Oxygenated Compounds/
Benzene
Fuel
IR (RFG Inspector's Manual)
Air

PAHs
Soil/Sediment
Immunoassay (EPA Method
4035)
Superfund

PCBs
Air, Wipes
GC/ECD (EPA Method
3508A)
Air/Superfund

Pentachlorophenol
Soil, Sediment
Immunoassay (EPA Method
4010)
Superfund

Pesticides/PCBs
Water, Soil, Sediment,
Waste (drum)
GC/ECD (EPA Method
8081 A/8082)
Superfund

Water, Soil, Sediment,
Waste (drum)
GC/ECD (EPA Method 680)
Superfund

Pharmaceuticals and Personal
Care Products (PPCPs)
Water
LC/MS/MS
Water

VOCs
Air (mini-cans)
GC/MS (EPA Method TO-15)
Superfund

Water, Soil, Air
GC/ECD/PID
Superfund

PHYSICAL AND OTHER DETERMINATIONS
Grain Size
Soil, Sediment
Sieve (Modified ASTM)
Superfund, Water
Region 1 SOP
Loss on Ignition (LOI)
Sediment

Water

Percent Lipids
Tissue
Gravimetric


BIOLOGY/MICROBIOLOGY
Enterococci
Ambient water
Enterolert
Ambient monitoring

Chlorophyll a
Ambient water
EPA 445.0
Ambient monitoring

Toxicity (Acute)
Sediment
C. dilutus, H. azteca
Water, Superfund
Bulk sediment

-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Unique Capabilities — FY2015
Analyte/Group Name
Sample Media
Analytical Technique
Supported Program(s)
Comments
INORGANIC CHEMISTRY
CO
Air/N2
EPA Reference or Equiv.
Method as in 40 CFR Part 58
Air

NOx
Air/N2
EPA Reference or Equiv.
Method as in 40 CFR Part 58
Air

S02
Air/N2
EPA Reference or Equiv.
Method as in 40 CFR Part 58
Air

Percent Sulfur
Fuel Oil
ASTM D4294
Air

Vanadium
Fuel Oil
ICP/AES
Air
Dry ashing at 525° C
REGION 2
ORGANIC CHEMISTRY
Asphaltenes (Hexane
Insolubles)
Fuel Oil
ASTM 3279
Air

Methane, Ethane, Ethene
Water
GC/FID
SF/RCRA

Ozone Precursors
(hydrocarbons)
Air
GC/MS/FID
Air

Pesticides
Wipes
LC/MS/MS and GC/MS
General

Total Petroleum Hydrocarbons
Water, Solid
Hexane Extraction (EPA
Method 1664)
Water

PHYSICAL AND OTHER DETERMINATIONS
Density
Ink, Paint
ASTM D1475
Air

Grain Size
Solid
Pipet Method
Superfund, Water

Solid
Hydrometer Method (based
on ASTM D422-63)
Superfund, Water

Particulates (Fine)
Air
EPA Reference or Equiv.
Method as in 40 CFR Part 58
Air

Percent Volatile Matter

ASTM D2369
Air

Percent Water
Ink, Paint
ASTM D4017
Air

Viscosity
Fuel Oil
ASTM D88
Air






BIOLOGY/MICROBIOLOGY
Cryptosporidium
Water
'Fluorescent Microscopy (EPA
Method 1623)
Water

DNA - qPCR (Enterococcus)
Water (Fresh & Marine)
EPA/Cepheid Methodology
Water

DNA-qPCR E. coli
Water (Fresh & Marine)
EPA/CDC Protocols
Water

DNA, Markers Various
Water (Fresh & Marine)
Geese, Gull, Cow, HF183,
Gen Bacteroidales
Water

Enterococcus Group
Water
Membrane Filtration
Water

Giardia
Water
Fluorescent Microscopy (EPA
Method 1623
Water

mColiblue24
Water
MF/Hach
Water

Enterolert w/ Quantitray
Water
Defined Substrate Technology
Water

Colilert 18/Colilert w/Quantitray
Water
Defined Substrate Technology
Water



36
EPA-910-R-17-001

-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Unique Capabilities — FY2015
REGION 3
Analyte/Group Name
Sample Media
Analytical Technique
Supported Program(s)
Comments
ORGANIC CHEMISTRY
Nitroaromatics & Nitroamines
Water, Soil/Sediment
HPLC
Water
Method 8330
Nitroglycerine
Water, Soil/Sediment
HPLC
Water
Method 8332
Nitrogen, Total
Water
Colorimetric
Emergency Response

Chemical Warfare Agents
Water/Solid/Wipe
GC/MS


PCB Congeners
Water, Soil/Sediment,
semi permeable
membrane device
(SPMD)
HR GC/MS

Method 1668C
PHYSICAL AND OTHER DETERMINATIONS
ID Ozone Depleting
Compounds
Propellants/Aerosols
FTIR
Air Enforcement

ID Unknowns
Bulk Mercury
Density
Superfund, RCRA

Water
FTIR
Water
Screening it, identify
unknowns
Soil/Sediment
FTIR

Screening it, identify
unknowns
Alcohols
Water, Soil/Sediment
FTIR
RCRA
When necessary for
Ignitability
ID Unknowns
Wastes
FTIR

Screening it, identify
unknowns





BIOLOGY/MICROBIOLOGY
Benthic Macroinvertebrate
Freshwater
Identification
Water

Marine/Estuarine Benthic
Invertebrate Taxonomy
Invertebrate Specimens
or Unsorted Sediment
EPA EMAP Protocols

Organisms identified to
species or lowest taxonomy
possible





REGION 4
INORGANIC CHEMISTRY
Chromium (+6)
Soil/Sediment
Std Method 3500 CrD
DW, Superfund

Mercury, Total - Ultra Low
Detection Level
Water
CVAF
Water
Method 1631
Tissue
CVAF
Water, Superfund
Appendix 1631
Soil/Sediment
CVAF
Water, Superfund
Appendix 1631
Metals, Total
Waste (oil, drum, etc.)
ICP/MS
RCRA
Not Commonly Available
Air
Hi-Vol Filters
Air
Not Commonly Available
Metals (TCLP)
Soil/Waste (oil, drum)
ICP/MS
RCRA
Not Commonly Available





ORGANIC CHEMISTRY
Freon Products
Canister & Air
GC/MS
Air, OECA
Special analysis technique
developed for criminal
investigations of illegal Freon
Natural Attenuation Analytes
Water
HR GC/MS (EPA Method
1668A)
Superfund
Methane, ethane, ethene
PCB Congeners
Water
HR GC/MS (EPA Method
1668A)
Superfund
High resolution GC/MS
Soil/Sediment
HR GC/MS (EPA Method
Superfund, RCRA
High resolution GC/MS
Tissue
HR GC/MS (EPA Method
Superfund, RCRA
High resolution GC/MS
Toxaphene Congeners
Water/Soil
GC/NIMS (EPA Method 8276)
Water, Superfund
6 Parlars, 2 breakdown
products
Ultimate BOD
Water
Membrane Electrode (Std
Method 5210C)
Water

BIOLOGY/MICROBIOLOGY
Chlorophyll
Water

Water

I

-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Unique Capabilities — FY2015
REGION 5
Analyte/Group Name
Sample Media
Analytical Technique
Supported Program(s)
Comments
INORGANIC CHEMISTRY
Bromide/Chloride Ratio
Brine Samples
IC & related characterization
techniques; ion balance
Water, UIC & SDWA
Difficult analyses
Chloride
Soil/Sediment
IC
Sediment

Metals
Suspended Particulate
Matter
ICP/MS
Air
Analysis of TSP, PM10,
PM2.5 filters for metals
ORGANIC CHEMISTRY
Nonylphenol (NP), NP-1 and 2-
ethoxylate, octyl phenol &
bisphenol-A
Water
GC/MS (ASTM D7065-11)
Water
Endocrine disruptor - High
Concentration method (ppb)
Nonylphenol (AP), AP-1 and 2-
ethoxylate, octyl phenol &
bisphenol-A
Soil/Sediment
GC/MS (8270 modified /
Internal SOP)
Water
Endocrine disruptor
Nonylphenol (NP), NP-1 and 2-
ethoxylate, octyl phenol
Water
LC/MS/MS (ASTM D7485-09)
Water
Endocrine disruptor Low level
method (ppt)
Bisphenol-A
Water
LC/MS/MS (ASTM D7574-09)
Water
Endocrine disruptor Low level
method (ppt)
Nonylphenol carboxylates
Water
LC/MS/MS
Water
Endocrine disruptor
Long chain NP, NPEOs (n=3-
18)
Water
LC/MS/MS (ASTM D7742-11)
Water
Endocrine disruptor
COD
Soil/Sediment
Colorimetric


PCBs
Water, Oil, Soil, Wipes
8082 (GC/EC)
TSCA
Aroclor specific TSCA reg.
Compliance method &
multiple action levels
PCB Congeners
Water. Sludge
GC/MS/MS, GC/NCI-MS
RCRA, SF, TSCA, Water
Compare with HRGC/HRMS
method
Chlorthalonil
Water
GC/MS
FIFRA
Stream Survey
Purgeable 1,4-Dioxane &
Tetrahydrofuran (THF)
Water
Method 624-Dioxane (Wide-
Bore Capillary Column GC/
MS)
Superfund
Specific analyte analysis
method
Various analytes (VOAs,
SVOCs & Pesticides/PCBs)
Water, Soil/Sediment
ESAT FASP Methods GC/EC
for VOAs, SVOCs &
Pesticides/PCBs (XRF for
metals)
Superfund
'Fast TAT on-site; Screening
or better data; Fast extraction
for organics
Toxic Industrial Chemicals
(TICs) & CWAdegradants
Drinking Water
LC/MS/MS Library Screening
WSD, NHSRC
Library search routine
developed under CRADA
with Waters Corp. Now use
NIST LC/MS/MS Library of
over 2,000 analytes
Aldicarb, aldicarb sulfone,
aldicarb sulfoxide, carbofuran,
oxamyl, methomyl and
thiofanox
Water
LC/MS/MS, ASTM7645-10
NHSRC
SAP Method
Aldicarb, bromadiolone,
carbofuran, oxamyl, and
methomyl
Water
LC/MS/MS, ASTM7600-09
NHSRC
SAP Method
Thiodiglycol
Water
LC/MS/MS, CRL SOP MS015
NHSRC
SAP Method
Soil
LC/MS/MS, ASTM E2787-11
NHSRC
SAP Method
Wipes
LC/MS/MS, ASTM E2838-11
NHSRC
SAP Method

-------

U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Unique Capabilities — FY2015
REGION 5 - continued
Analyte/Group Name
Sample Media
Analytical Technique
Supported Program(s)
Comments
INORGANIC CHEMISTRY
Diethanolamine,
triethanolamine, n-
methyldiethanolamine and
methyldiethanolamine
Water
LC/MS/MS, ASTM D7599-09
NHSRC
SAP Method
Dioctyl Sulfosuccinate (DOSS)
in Seawater
Stormwater
LC/MS/MS, ASTM D7730-11
NHSRC/SF
SAP Method
Dipropylene glycol monobutyl
ether and ethylene glycol
monobutyl ether in seawater
Stormwater
LC/MS/MS, ASTM D7731-11
NHSRC/SF
SAP Method
Bromodiolone, brodifacoum,
diphacinone and warfarin in
water
Water
LC/MS/MS, ASTM D7644-11
NHSRC
SAP Method
Diisopropyl
methylphosphonate, ethyl
hydrogen
dimethylamidophosphate, ethyl
methylphosphonic acid,
isopropyl methylphosphonic
acid, methylphosphonic acid
and pinacolyl
methylphosphonic acid
Water
LC/MS/MS, ASTM 7597-09
NHSRC
SAP Method
DIMP, EMPA, IMPA, MPA,
PMPA
Soil
LC/MS/MS, ASTM WK34580
NHSRC
SAP Method





PHYSICAL AND OTHER DETERMINATIONS
Corrosivity by pH
Hazardous Waste
SW846 1110
RCRA
Waste characterization
Particle Size
Soil/Sediment
Particle size analyzer
provides continuum of sizes-
CRLSOP
GLNPO, Water- Sediment
For modelling and soil
migration calcs.
Water Content
Hazardous waste
SW846 -9000
RCRA, Superfund
Support for flashpoint
Paint Filter Test
Paints and coatings

RCRA, Superfund

Specific Gravity
Soil/Sediment
Appendix IV of the Corps of
Engineers Engineering
Manual (F10-F22)
Sediment

I

EPA-910-R-17-001
39
II

-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Unique Capabilities — FY2015
REGION 6
Analyte/Group Name
Sample Media
Analytical Technique
Supported Program(s)
Comments
INORGANIC CHEMISTRY
Ammonia
Air (passive coated
filter)
IC
CAA
Ogawa passive air collection
device
Ozone
Air (passive coated
filter)
IC
CAA
Ogawa passive air collection
device
NOx
Air (passive coated
filter)
IC
CAA
Ogawa passive air collection
device
SOx
Air (passive coated
filter)
IC
CAA
Ogawa passive air collection
device
Trace level Hex Chrom
Water
IC/UV
Water

Perchlorate
Water
IC/MS/MS
Water

Metals by X-Ray Fluorescence
Soil
portable XRF
Superfund, RCRA
field screening
ORGANIC CHEMISTRY
Incidental PCBs
Water
GC/MS; Method 680
Homologue Series
TSCA, RCRA
grouped by number of
chlorine
Soil/Sediment
GC/MS; Method 680
Homologue Series
TSCA, RCRA
grouped by number of
chlorine
Waste
GC/MS; Method 680
Homologue Series
TSCA, RCRA
grouped by number of
chlorine
Chemical Warfare Agents
PAMS (C2s and C3s identified)
Water/Sol id/Wi pes
GC/MS
Emergency Response

Air
GC/MS/FID (split)
CAA
C2s and C3s are individually
quant itated
PCBs (Aroclor)
Electrical Cable
GC; Separation, extraction,
analysis of individual
components. Mod of program
specific technique
TSCA
Toluene is extraction solvent
PAHs (trace)
Water/Solid/Oil
GC/QQQ
RCRA, Superfund

Chemical Warfare Agents-
Degradation products
Water
LC/MS/MS
Emergency Response

VOCs by OVM
Air
GC/MS
CAA
passive air monitoring
Alcohols by headspace
Water
GC/MS
RCRA, Superfund

Light Hydrocarbons (dissolved
gases)
Water
GC/MS
RCRA, Superfund

Organophosphorous Pesticides
(OPPs)
Water
GC/NPD
RCRA, Superfund

Soil/Sediment
GC/NPD
RCRA, Superfund

Waste
GC/NPD
RCRA, Superfund






PHYSICAL AND OTHER DETERMINATIONS
Corrosivity by pH
Waste
Method 1110 - Corrosivity
Toward Steel
RCRA


EPA-910-R-17-001

-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Unique Capabilities — FY2015
REGION 7
Analyte/Group Name
Sample Media
Analytical Technique
Supported Program(s)
Comments
INORGANIC CHEMISTRY
CD
Air
40 CFR Part 58
Air
OAQPS Protocol Gas
Verification Program
NOx
Air
40 CFR Part 58
Air
OAQPS Protocol Gas
Verification Program
S02
Air
40 CFR Part 58
Air
OAQPS Protocol Gas
Verification Program
03
Air
40 CFR Part 58
Air
NIST Standard Reference
Photometer
In-vitro Bioaccessibility Assays
for Arsenic and Lead in Soil
Soil
ICP-MS/ICP-AES
Superfund/RCRA
SUPR Exposure/Toxicity
Assessment
ORGANIC CHEMISTRY
Chlordane
Air(PUF)
GC/ECD (EPA Method TO-
4A)
Special Project

Herbicides
Water, Soil/Sediment
GC/ECD
Water
Use Attainability Analysis
(UAA)
Pesticides
Water, Soil/Sediment,
Tissue
GC/ECD
Water
Use Attainability Analysis
(UAA)
VOCs
Air Canister
GC/MS (EPA Method TO-14
&TO-15)
Air/Superfund
Air Toxics
Air Sorbent Tube
GC/MS (EPA Method TO-17)
Air/Superfund
Air Toxics
Water
GC/MS
Superfund/ORD
In-Situ Chemical Oxidation
Site Support
PCBs
Soil/Sediment, Waste
GC/ECD
Superfund/ORD
Rapid Site Screening
Pharmaceuticals and Personal
Care Products (PPCPs)
Water
LC/MS/MS
Water
Endocrine disruptors
PAHs, Pesticides, Herbicides
Water
Twister GC/MS Stir Bar
Sorptive Extraction
(solventless extraction)
Water
Use Attainability Analysis
(UAA)
VOCs
Water, Soil, Air
GC/MS Mobile Laboratory
Superfund
Rapid Site Characterization
VOCs from In-situ Chemical
Oxidation Sites
Water
GC/MS
Superfund
Improved Precision of VOC
Samples from In-situ
Chemical Oxidation Sites
BIOLOGY/MICROBIOLOGY
E. coli
Water (drinking/waste/
ambient)
qPCR
Water
2008 NFWA
Enterococci
Water
qPCR
Water

Heterotrophic Bacteria
Water
Plate Count - Standard
Methods
Water
2008 NFWA
Chlorophyll a
Ambient water
EPA 445.0
Ambient monitoring

Invertebrate Taxonomy
Invertebrates
EPA EMAP Protocols
Water

Marine/Estuarine Benthic
Taxonomy
Benthic Organisms

Water
Organisms identified to
species or lowest taxonomy
possible

-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Unique Capabilities — FY2015
REGION 8
Analyte/Group Name
Sample Media
Analytical Technique
Supported Program(s)
Comments
INORGANIC CHEMISTRY
Silica
Water
Colorimetric
Water/Superfund

Gadilinium
Water
ICP/MS
Water/Superfund
Wastewater Indicator





ORGANIC CHEMISTRY
Alcohols
Water
GC/FID


Chlorophyll
Water
HPLC
Water/Superfund

Endothall
Water
GC/MS
Water/Superfund

TPH (VOA & BNA)
Water, Soil/Sediment
GC/MS or GC/FID
Water/Superfund

LC/MS/MS Pesticides
Water
LC/MS/MS
Water/Superfund
Monitoring for States and
Tribes
Low Level Pesticides/CLLE
Water
GC/MS
Water/Superfund
Monitoring for States and
Tribes
Metals - Arsenic/Selenium
speciation
Water, Soil, Tissue
IC/ICP/MS
Water/Superfund
Speciation data needed for
risk assessment
Pharmaceuticals and Personal
Care Products (PPCPs)
Water
IC/ICP/MS
Water/Superfund
Endocrine disruptors
Waste Indicator Compounds
Water
GC/MS
Water/Superfund
Monitoring for States and
Tribes
Total Petroleum Hydrocarbons-
Diesel Range Organics
Water, Soil
GC/FID
Water/Superfund
Hydro-Fracking
VOAs
Water, Soil/Sediment
GC/PID/ELCD
Water/Superfund

BIOLOGY/MICROBIOLOGY
Bacteria (Arsenic-Reducing)
Water, Sediment
MPN
Water/Superfund

Bacteria (Iron-Reducing)
Water, Sediment
MPN
Water/Superfund

Bacteria (Sulfate-Reducing)
Water, Sediment
MPN
Water/Superfund

Bacteria (Clostridium
perfringens)
Water
Membrane Filtration
Water/Superfund

Water
Membrane Filtration
Water/Superfund


42
EPA-910-R-17-001


-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Unique Capabilities — FY2015
REGION 9
Analyte/Group Name
Sample Media
Analytical Technique
Supported Program(s)
Comments
INORGANIC CHEMISTRY
Ferrous Iron
Water
Titration with Dichromate
Superfund

Mercury, Vapor, Particulate and
Reactive
Ambient Air
Cold Vapor Atomic
Fluorescence
Air, Water (TMDL)

Methyl mercury
Water
CVAF (EPA 1630)
Water

Metals (with mercury)
Dust wipes, Ghost
wipes
ICP, ICPMS, CVAA
Tribal Program

Metals (SPLP)
Soil, Sediment, Solid,
Waste, Tissue
SW846 1312: ICP, GFAA,
CVAA, I CP/MS
Superfund, RCRA

Low level hexavalent chromium
Drinking Water
IC with post column reaction/
UV detection
Water

Metals
Soil
Portable XRF
Superfund, Criminal
Investigation

Platinum Group Metals
Catalytic converter
washcoat
Portable XRF
Enforcement, Air

Lead (Pb) in Air
TSP High-Volume filters
FEM EQL-0710-192, ICP/MS
Air
New Pb NAAQS
Perchlorate
Water, Soil
LC/MS/MS (EPA Method
331.0)
Superfund/Water

In vitro bioaccessibility assays
for arsenic and lead in soil
Soil
EPA 9200.1-86
Superfund

ORGANIC CHEMISTRY
Diazinon
Water
ELISA
WQM

1,4-Dioxane
Water, Soil, Sediment
GC/MS
Superfund, RCRA

EDB/DBCP
Water
GC (EPA 504.1)
Superfund, RCRA

Methane, Ethane, Ethene
Water
GC/FID (RSK-175)
Superfund, RCRA






BIOLOGY/MICROBIOLOGY
Benthic Taxonomic
Identification
Sediment (Marine)
Taxonomic Identification
Water, WQM

Chlorophyll/Pheophytin
Water/Periphyton
Standard Method 10200 H,
Procedure 2b
Water, WQM

Enterococci
Water
Enterolert
Water, NPDES, WQM

Heterotrophic Bacteria
Water
Plate Count - Standard
Methods
Water, NPDES, WQM

Microcystin
Water
Immunoassay
Water

Toxicity Test, Red Abalone
(Haliotis rufescens) Larval
Development
Water
EPA/600/R-95/136
NPDES

Toxicity Test, Sea Urchin
Fertilization [Stronglyocentrotus
purpuratus]
Water
EPA/600/R-95/136
Water, NPDES

I
EPA-910-R-17-001
43

-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Unique Capabilities — FY2015
REGION 10
Analyte/Group Name | Sample Media Analytical Technique | Supported Program(s) | Comments
INORGANIC CHEMISTRY
Asbestos, Bulk
Solids
EPA 600/R93/116 - XRD
Superfund

Low Level Mercury
Water
CVAF, Method 1631E
Water, Superfund
0.2 to 0.5 ng/L reporting limits
Methyl Mercury
Water
GC/CVAFS, Method 1630
Water, Superfund

Metals
Air filters
ICP/MS, ICP
CAA

Blood
ICP/MS
Superfund

Soil
Portable XRF
Superfund, Criminal
Screening results for metals
Paint
Portable XRF
TSCA, Criminal
Lead in paint
Solid
X-Ray Diffractometer (XRD)
Superfund
Characterizes the form metals
exist in sample
Metals - Arsenic speciation
Fish/shellfish/seaweed
IC/ICP/MS
Superfund, Water
Speciation data needed for
Metals (TAL) + Total Uranium
Small mammals,
invertebrates
Microwave Digestion, ICP/
AES, ICP/MS
Superfund, RCRA
Biomonitoring projects
Metals (SPLP)
Soil/Waste
ICP/AES, ICP/MS
Superfund

Chlorophyll a
Water
SM 1002H
Water

In-vitro Bioaccessibility Assays
for Lead in Soil
Soil
Leachates by Method 1340,
IC/AES
Superfund

Percent Water
Liquid Waste
Karl Fischer titration
RCRA

Perchlorate
Produce (fruits, milk)
IC/MS
Superfund

ORGANIC CHEMISTRY
BNA (Selected)
Tissue
SW846 Methods
Superfund

Butyl/tins
Soil/Sediment
GC/MS
Superfund, Criminal
WDOE method
1,4-Dioxane
Water
EPA Method 8270D SIM/
Method 522
Superfund

Explosives (Nitroaromatics &
Nitroamines)
Water, Soil, fish/
shellfish
EPA Method 8330/HPLC
Superfund

Hydrocarbon Identification
Water, Soil/Sediment
NWTPH-HCID
Superfund, Criminal

N-Nitrosodimethylamine
Water, Soil
Method 521
Superfund

Herbicides
Water, Soil/Sediment
GC/MS
Superfund

Polybrominated diphenyl ethers
(PBDEs)
Water
GC/MS Low Resolution
Water

Sediment/bio solids
GC/MS Low Resolution
Superfund, Water

Tissue (fish)
GC/MS Low Resolution
Superfund

Total Petroleum Hydrocarbons-
Gasoline Range Organics
Water, Soil
NWTPH-Gx
Superfund, RCRA

Water, Soil
NWTPH-Dx
Superfund, RCRA

VOAand SVOA
Industrial wastes,
Solids, Tissues
Vacuum distillation, Methol
8261A
Superfund, RCRA

Low Level Polyaromatic
Hydrocarbons and Other
Neutral Organics
Soil, Sediments
GC/MS-MS
Superfund, Brownfields,
Water

PCB aroclors
Wipes
GC/ECD
Brownfields, RCRA

Low Level Polyaromatic
Hydrocarbons
Shellfish, Water
GC/MS-MS
Superfund, Brownfields











PHYSICAL AND OTHER DETERMINATIONS
Incremental Sampling
Methodology (ISM) Preparation
of Soil Samples for Organic and
Inorganic Analyses
Soil
Described in Method 8330B
Appendix
Superfund, Brownfields

Variety of water quality tests
Water
Various probe-type
measurements
Superfund
Flow thru cell system;
performed in the field





BIOLOGY/MICROBIOLOGY




Aeromonas spp
Drinking Water
EPA Method 1605
SDWA- Unregulated
Contaminant Monitoring Rule
(UCMR)
EPA Approved
Cryptosporidium and Giardia
Water
EPA Method 1623 (Filtration/
IMS/Staining)
SDWA, Water, Ambient
Monitoring Rule - recreational
waters
On approval list for LT-2
regulation
Enterococci
Ambient Water
EPA Method 1600
Ambient Monitoring Rule

Microbial Source Tracking
Water
PCR
Water

Microscopic testing
Drinking/Source Water
Microscopic particulate
analysis
Surface Water Treatment
Rule
Microscopic technique used to
establish GWUDI
characteristics of drinking

-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Developing Capabilities — FY2015
REGION 1
Project Method
Developmental Need
Status
Projected Completion
Enterococcus in water by qPCR (EPA
Method 1611 capability development
Water
In progress
FY2014
REGION 2
SIM analysis for VOA and semi-VOA
analysis
Drinking and Surface Water
Developing methods on current
instrumentation
FY2015
REGION 3
Arsenic speciation for water, soil/
sediment & tissue by IC or ICP/MS
Speciation data to be used for risk
assessments in support of Clean
Water Act and Superfund.
Identified developmental need; initiated
research and evaluation of analytical
procedures; project placed on hold due
to lack of demand for analysis
On hold
EPA Method 1694 for Pharmaceuticals
and Personal Care Products (PPCP) by
LC/MS/MS
Need for capability to identify and
quantify pharmaceutical and
personal care products.
Cancelled due to loss of analyst.
On hold
PCR quantitation and source tracking
Need for capability to determine
source of E.coli contamination in
support ofWater Program.
In-progress.
FY2013
Glycols in water
Need for capability to identify glycol
compounds in groundwater using
LC/MS/MS to achieve lower
quantitation limits.
Complete
FY2013
Enzyme-Linked Immunosorbent Assay
(ELISA)
Need for in-field testing of surface
and drinking water for presence of
estrogen and estrogen-like
compounds.
Complete
FY2013
Dissolved Gases
Superfund
Initiated. Method 537

SVOCs in drinking water
Drinking Water
Initiated. Method 537

REGION 4
EPA Method 8261
VOCs in difficult matrices
Initial investigation
Unknown
Internal Method - GC/MS/MS
Low-level pesticides w/MS confirm
Institute of Technology and Marine
Engineering (ITME) is in process
Jan-15
Lead bioavailability in soil, Method
1340
Superfund
continued work

REGION 5
PFOA/PFOS in biosolids and water
Water Division study - Resource
Management Information System
(RMIS)
Initial work done, new instrument
installed and standards run to set up
instrument. SOP in draft.
FY2016
qPCR, Gene Sequencing Guar Gum
Hydrofracking (HF) fluid screening
tool - Region 3 support
Some samples sequenced, screening
tool in process.
FY2016
Glyphosate in water by IC/MS
Pesticide program request for
stream survey
Method development completed, SOP
in process.
FY2016
Fluorotelomer alcohols in water by MS/
MS
Water
Initiated. SOP in draft.
FY2016
Glyphosate by IC/MS
Pesticides
completed

Pesticide screen by LC/MS/MS
Pesticides
completed

Low-level 1,4-Dioxane by GC/MS
Superfund
initiated

PCB congeners by GC/MS/MS
Great Lakes National Program
Office (GLNPO)
completed

LC/MS methods
ORD
completed


-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Developing Capabilities — FY2015
REGION 6
Project Method
Developmental Need
Status
Projected Completion
Anions and Oxyhalides by IC
Remove dependence on State Lab
for this test. Superfund, RCRA,
CWA
Method developed, need DOC/MDL;
SOPs,. continued
April 2016
Direct mercury analysis (CVAF -
Milestone)
CWA, RCRA, Superfund
DOC/MDL; SOP preparation,
continued
December 2016
Expanded 8270 list by GC/QQQ
RCRA, Superfund
Continued method development
December 2016
High Dissolved Solids/Modified Method/
Anion
CWA RCRA, Superfund
Method being developed, continued
October 2016
High Dissolved Solids /Modified Method/
Cation
CWA, RCRA, Superfund
Method being developed, continued
October 2016
High Dissolved Solids/Modified Method/
OA
CWA, RCRA, Superfund
Method being developed continued.
January 2017
Pharmaceuticals and Personal Care
Products (PPCP) analysis
CWA
Method being developed, continued
August 2016
Passive formaldehyde
Clean Air Act (CAA)
Method being developed, continued
On hold
Asbestos
Superfund, RCRA
On hold

Induction-coupled plasma axial method
Superfund
continued

Low molecular weight acid in resource
extraction analysis
Safe Drinking Water ACT (SDWA)
continued

Haloacetic acids in resource extractor
analysis
SDWA
continued

REGION 7
EPA Method 1694 for (PPCP) by HPLC/
MS/MS-Direct injection analysis-direct
Speciation data to be used for Risk
Assessments in support of Clean
Water Act and Superfund.
Performing method validation studies
on surrogate compounds; developing
SOP, expanded list of targets in
FY2015 and FY2016. continued work
FY2016
Pesticides by GC/MS/MS
Confirmational analysis of pesticide
analytes previously performed by
GC/ECD CWA RCRA Superfund
Instrument installed, method
development and validation pending
nitiated
FY2016
Microbial Source Tracking Using qPCR
TMDL and Stormwater
Non human marker test completed;
Pending additional technical method
guidance from ORD continued work
FY2015
Arsenic speciation for water, soil/
sediment & tissue by IC or ICP/MS
Speciation data to be used for Risk
Assessments in support of Clean
Water Act and Superfund.
Method development currently
underway, on hold
On hold due to limited staff.
EPA Method 1694 for PPCP by HPLC/
MS/MS-direct injection analysis.
Speciation data to be used for Risk
Assessments in support of CWA
and Superfund. Water Program
Sample analysis for Urban Stream
Monitoring, continued improvements.
Ongoing
PAH/SVOC in Water by Stir Bar
Sorptive Extraction
Water Program
Sample analysis for Urban Stream
Monitoring, continued improvements.
Continued work pending final Code of
Federal Regulations (CFR)
Ongoing
Airborne VOC by Solid Sorbent Tube
(EPA Method TO-17)
Air Program
Air sample monitoring for ongoing sites
with regular re-evaluations. Use three-
ohased sorbent tubes for low to
moderate humidity.
FY2015, field tests in FY2016
Airborne VOC by Solid Sorbent Tube
(EPA Method TO-17)
Air Program, vapor Intrusion
Developing a single-phase sorbent
method for evaluating high humidity
uses for vapor intrusion and cave air
evaluations. This method will focus on
a short list of chlorinated VOCs
FY2016
Independent Laboratories Institute Solid
-Phase Extraction Study
Office of Water
Participation in the second phase of
the study using two different solid
ohase extraction techniques: Horizon
SPE, and Stir Bar Sorptive Extraction
FY2016

-------
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Developing Capabilities — FY2015
REGION 8
Project Method
Developmental Need
Status
Projected Completion
Asbestos / Electron Microscope
Need for capabilities to analyze
water and soils for asbestos
contamination at Superfund sites.
Instrument operational and running
samples.
Ongoing
Endocrine Disruptor Studies / LC/MS/MS
Emerging needs for the Water
orogram and ORD.
Performing method validation.
Ongoing
Macroinvertebrate - Freshwater
Benthic / Manual Enumeration
Redevelop capability for Water
orogram support due to loss of
staff.
Planning to hire replacement staff.
Ongoing
Microbial Source Tracking
Develop capabilities in this
technology for use in projects and
emerging needs for the Superfund,
Water programs and ORD.
Biolog system installed; some staff
trained; assessing method.
Ongoing
Microbial Source Tracking by PCR
Develop capabilities in this
technology for use in projects and
emerging needs for the Water,
Enforcement programs and ORD.
Instruments and sample processing,
ESAT staff training and/or assessing
methods.
Ongoing
Arsenic Speciation for Water, Soil/
Sediment & Tissue/ IC/ICP/MS
Speciation data to be used for Risk
Assessments in support of Clean
Water Act and Superfund.
Identified developmental need; initiated
research and evaluation of analytical
procedures; necessary modifications to
laboratory in progress.
Ongoing
Toxicity - Acute & Chronic in Mobile Lab
On-site assessment for potential
needs by the Water program.
Mobile lab available; team lead
initiating discussion of projects and
team development.
Ongoing
Pharmaceuticals by LC/MS/MS
Water and ORD
Progress continuing.
Ongoing
Pesticides by LC/MS/MS
Water
Progress continuing.
Ongoing
Hormones and Steroids by LC/MS/MS
Water and ORD
Progress continuing.
Ongoing
Algal Toxins
Water
completed

REGION 9
Low level total mercury in water (EPA
1631E)
Address regional priority.
Instrumentation installed. Method
development initiated.
FY2015
Analysis of Radiello passive air samplers
for VOCs
Address regional priority.
Assessing health and safety issues
FY2015
Anatoxin-a analysis using Receptor
Binding Assay (RBA)
Water Program monitoring for
cyanotoxins
Method development initiated. No new
equipment needed. SOP drafted.
Early FY2016
Analysis of VOCs in air using passive
diffusive sampling media
Superfund (vapor intrusion)
completed
FY2015
Precious metals analysis of washcoat
from automobile catalytic converters
using a Niton ZL3t Field-Portable X-Ray
Fluorescence (FPXRF)
Air enforcement
completed
FY2015
REGION 10
Develop Methyl Mercury Analysis
Capability for Sediment Samples
Methyl mercury data needed to
support regional mercury strategy
toward characterizing levels in the
environment and evaluate public
nealth risks.
Some initial testing on instrument
conducted. Based on the effort
needed to develop the water method,
capability for sediment analyses will
likely require much experimentation
with the Brooks-Rand instrument to
acquire the needed accuracy and
sensitivity for sediments.
Progress delayed due to workloads
and program needs are uncertain.
Ultra-trace Concentration Phosphorus
Method for Treated Wastewater Effluent
and Surface Water
NPDES compliance monitoring at
ultra low phosphorus levels.
Ultra-trace standard concentration
measurements were achieved on a
Lachat colorimetric instrument and an
ICP-MS system. Testing on actual
effluent samples still to be planned.
Progress delayed due to
workloads. Method developed but
validation through matrix testing still
to be done. New expected
completion date to be determined.

-------
I
U.S. EPA Regional Laboratory System
FY2015 Annual Report
Regional Laboratories Developing Capabilities — FY2015
REGION 10 - continued
Project Method
Developmental Need
Status
Projected Completion
Develop Diffusive Thin-Film Gradient
(DGT) Preparation and Arsenic Analysis
Capability
The DGT disks are being tested at
a Superfund site to determine if the
material can effectively mimic
arsenic uptake of bivalves in
marine sediment. The DGTs
require special conditioning at the
aboratory and the arsenic analysis
method needed to be developed
for this matrix.
The development of the methods was
nitiated in 2015. Actual use of the
disks and comparisons to actual data
of clams are scheduled for early 2015.
FY2015
Develop Pesticides Analysis Capability
for Wipe Samples
Wipe samples are planned to be
collected at various tribal childcare
facilities in OR to test for pesticides
during CY 2016.
The pesticides were identified and a
iterature search of methods was
conducted.
FY2016
Methyl Mercury Analysis for Sediment
Samples
Superfund, water
continued work

Develop/Mobilize Activity Analysis
Capability by SM2310GB in Water
Samples
Superfund, water
nitiated

Develop New extraction Methods for
Pyrethroids and other common
pesticides in wipe samples
Enforcement and ORD
nitiated


48
EPA-910-R-17-001


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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Appendix A — Acronyms/Abbreviations
vvEPA
United States
Environmental Protection
Agoncy

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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Acronyms/Abbreviations
-A-
ACHD
Allegheny County Health Department
ACIL
American Council of Independent Laboratories
ADEM
Alabama Department of Environmental Management




-B-
BTEX
benzene, toluene, ethylbenzene and xylenes




-C-
CAA
Clean Air Act
CERCLA
Comprehensive Environmental Response, Compensation, and Liability Act
CFR
Code of Federal Regulations
CLP
Contract Laboratory Program


-D-
DGT
diffusive thin-film gradient
DOC
demonstration of capability


-E-
EAID
Environmental Assessment & Innovation Division
EBCI
Eastern Band Cherokee Indians
EDXA
energy-dispersive x-ray analysis
EFED
Environmental Fate and Effects Division
EPA
Environmental Protection Agency
EPR
Office of Ecosystems, Protection and Remediation
ESA
Environmental Site Assessment
ESAT
Environmental Services Assistance Team


-F-
FBAS
fluidized bed asbestos segregator
FBT
Office of Monitoring and Assessment Freshwater Biology Team
FIT
FOG Implementation Team
FOG
Field Operations Group
FY
Fiscal Year




-G-
GC/FID
gas chromatography/flame ionization detector
GC/MS
gas chromatography/mass spectrometry


-H-
HA
Health Advisories
HC
hydrocarbon
HHRA
Human Health Risk Assessment
HRS
Hazard Ranking System



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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Acronyms/Abbreviations
-I-
ICP
inductively coupled plasma
ICP/MS
inductively coupled plasma/mass spectrometry
IR
infrared
ISM
incremental sampling methodology
ISO
International Standards Organization




-J-


-K-


-L-
LC
liquid chromatography
LDW
Lower Duwamish Waterway


LIMS
Laboratory Information Management System
LTAB
Laboratory Technology and Analysis Branch
-M-


MST
microbial source tracking






-N-
NEIC
National Enforcement Investigations Center
NELAC
National Environmental Laboratory Accreditation Conference
NOAA
National Oceanic and Atmospheric Administration
NPL
National Priorities List






-O-
OASQA
Office of Analytical Services and Quality Assurance
OEI
Office of Environmental Information
OPP
Office of Pesticide Programs
OPP-AD
Office of Pesticide Programs Anti-Microbial Division
OPRA
Office of Partnerships & Regulatory Assistance
ORD
Office of Research and Development
OTMS
Office of Technical & Management Services
-P-
PAH
polycyclic aromatic hydrocarbon
PCB
polychlorinated biphenyl
PID
photoionization detector
PLM
polarized light microscopy





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U.S. EPA Regional Laboratory System
FY2015 Annual Report
Acronyms/Abbreviations
-Q-
QA
Quality Assurance
QAFAP
QA Field Activities Procedure
QAPP
Quality Assurance Project Plan
QC
Quality Control


-R-
RARE
Regional Applied Research Effort
RCRA
Resource Conservation and Recovery Act
RLN
Regional Laboratory Network
RME
Reasonable Maximum Exposure


-S-
SEM
scanning electron microscopy
SESD
Science & Ecosystem Support Division
SIM
Selected Ion Monitoring
SIP
state implementation plan
SLAMS
State and Local Agency Monitoring Station
SME
subject matter expert
SPE
solid-phase extraction
STC
Science and Technology Center
SVOA
semi-volatile organic analysis
-T-
TCE
trichloroethylene
TEM
transmission electron microscopy
THM
trihalomethanes
TMDL
total maximum daily load
TOC
total organic carbon
TSS
total suspended solids
TTHM
total trihalomethanes
-U-


U.S. EPA
United States Environmental Protection Agency


-V-
VOA
volatile organic analysis
VOC
volatile organic compound


-w-
WPD
Water Protection Division
-X-
XRD
X-ray diffraction
XRF
X-ray fluorescence


-Y-


-Z-



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