822B17002
ALGAL INDICATORS IN STREAMS: A
REVIEW OF THEIR APPLICATION IN WATER
QUALITY MANAGEMENT OF NUTRIENT
POLLUTION
Michael J. Paul
Tetra Tech, Inc., Center for Ecological Sciences, Research Triangle Park, NC
Brannon Walsh, Jacques Oliver, and Dana Thomas
U.S. Environmental Protection Agency, Office of Water, Office of Science and Technology,
Washington, D.C.
1

-------
PREFACE
This paper summarizes the application of algae as indicators of nutrient pollution in water quality
management. It describes the use of algal indicators to develop water quality diagnostics for nutrient pollution in
the United States (U.S.) and then reviews scientific developments in the use and application of algal indicators
across the world. The paper is intended as a technical resource for the water quality manager/practitioner seeking
to utilize algae to detect the presence of nutrient pollution and to estimate the risks of nutrient pollution in
adversely affecting the condition of stream ecosystems.
INTRODUCTION
Algae are ubiquitous and essential components of all stream ecosystems (Stevenson 2014; Stevenson and
Smol 2003). They are the primary energetic source for many stream food webs, fixing carbon from the atmosphere
through photosynthesis, which is then transmitted through the web via consumer pathways. Algae are able to
produce this energy for stream ecosystems across a wide range of physical and chemical conditions, from hot
thermal spring fed stream ecosystems to cold, arctic stream ecosystems. They are represented by a vast range of
different species, growth forms, and life histories.
Algae have a long history of use and possess many of the features valued in ecological indicators. They were
part of the early saprobien indicator system development in Germany and were one of the first assemblages
developed for use in biological assessment in the United States (Stevenson 2014; Stevenson et al. 2010; Stevenson
and Smol 2003). Algae are relevant ecologically in streams and clearly impact the benefits that people obtain from
these ecosystems. Also algae can be feasibly measured. They are easily sampled and processed using a wide
variety of methods, and they can be identified for relatively low cost. Algal physiologies make them attractive for
investigating biological responses across a range of stressors and stressor variability. Algae exhibit a wide variety of
sensitivity/tolerance among their many naturally-occurring taxa. They respond quickly to disturbance and recover
quickly after a stressor is removed, and while they vary naturally, as do most aquatic organisms, that natural
variability can be quantified and factored into analysis. Lastly, algal measurements are readily interpreted and
understood by scientists, policy makers, and the public (US Environmental Protection Agency (USEPA) 2000).
The ecological importance and distinguishing features of algae, particularly as indicators of nutrient pollution,
make them conducive as assessment endpoints for numeric nutrient criteria development for water quality
management purposes under the Clean Water Act (USEPA 2000, 2014). This value lies in both their sensitivity to
nutrient pollution, as well as their linkage to aquatic life, drinking water source and recreational designated uses
(Stevenson and Smol 2003; USEPA 2000). Indicators that have been developed using algae include measures of
productivity, biomass and assemblage composition. Productivity measures include measures of photosynthesis
and respiration using chamber and open system methods. Biomass indicators include cell abundance, cell
2

-------
biovolume, photopigments (e.g., chlorophyll a), and ash free dry mass (AFDM). Assemblage composition measures
include taxonomic estimates of diversity, richness, and a suite of metrics characterizing an array of algal traits (e.g.,
pollutant sensitivity, motility).
Algal impacts on uses occur principally through the ecological phenomena of competition and productivity
(Figure 1). Algae are aquatic life and therefore directly measure that use; their importance as primary resources in
stream food webs means they are integrally linked to all other aquatic life, most directly higher trophic levels such
as invertebrates and fish. Competition among algae for nutrients means that enrichment by nutrient pollution
shifts the composition of the algal community, including edible forms, affecting food quality for higher trophic
levels. In addition, nutrient pollution increases primary productivity, affecting the amount of food available to
consumers whose composition is therefore altered in turn due to their competitiveness for food resources. In
addition to these direct effects on aquatic life, algal productivity also affects aquatic life indirectly through
dissolved oxygen and pH (through photosynthesis and decomposition of algal detritus) and physical habitat
(through excess growth altering feeding and reproductive habitat).
Algae can also impact other important designated uses, such as drinking water and recreation, by the same
pathways through which aquatic life uses are impacted. The competitive shift in species composition that occurs
with nutrient pollution favors nuisance and harmful algal bloom taxa that produce toxins or compounds
contributing to taste and odor issues that influence drinking water quality and treatment costs, as well as
recreational uses of a water body. Similarly, increased productivity increases the concentration of dissolved
organic compounds that contribute to disinfection by-product formation, and higher biomass can also increase
operational costs associated with filtration. Many nuisance taxa have growth forms that are less desirable for
recreation (e.g., long filaments and/or floating mats). High biomass also can affect water clarity making it
aesthetically less desirable and also more difficult to see through.
3

-------
Higher
Trophic Level
Competition
Toxins
Primary
Producer
Species
Shifts
Recreation
Nuisance/Harmful
Algae/Plants
Aesthetics
Drinking
Disinfection Byproducts
Productivity
Increased
N/P
Increased
Primary
Productivity
Increased
Organic
Matter
Increased
Respiration
Decreased
Oxygen
Higher
Trophic Level
Physical
Habitat
Proximate Stressor
Clarity
Recreation
Food Quality
Taste and Odor
Increased
N/P
Figure 1. Conceptual model of effects of algae on designated uses.
Development of protective numeric nutrient criteria relies on the availability of assessment endpoints that are
clearly responsive to nutrient pollution stress and linked to management goals. Algae are among the most
important indicators of nutrient pollution stress and risk to designated use impairment in streams. As a result, EPA
recommends that states and tribes consider the use of an algal biomass indicator, specifically chlorophyll a,
because algae are not only a scientifically sound direct response to nutrient pollution, but algae in excess also
stress aquatic ecosystems. At present, there is no comprehensive synthesis of the current state of diatoms and
non-diatom algae application in streams as indicators of nutrient pollution that has been developed to assist water
quality standards scientists and managers (See Stevenson (2014); Stevenson et al. (2010); Stevenson and Smol
(2003) for excellent academic review of the subject). Therefore, the purpose of this review is to provide a
reference document that details the current use of algae as indicators of nutrient pollution in streams to be helpful
to states and tribes in the development of numeric nutrient criteria. This review includes descriptions and
examples of the variety of methods and endpoints used, how algae are applied to assess biological condition and
derive numeric pollutant endpoints, and the extent of their application in the U.S.
This review is comprised of two sections: the first section is a summary of U.S. applications by state and
federal agencies; and the second section is a review of algal indicator research that has been pursued by scientists
principally outside of state and federal agencies to advance the use of algae in nutrient pollution applications.
4

-------
STATE AND FEDERAL AGENCY SUMMARY
EPA has encouraged states to use multiple assemblages, including algae, as part of the development of aquatic
life use criteria (i.e., biocriteria, USEPA 2011, 2013a). EPA has also encouraged states to specifically use measures
of primary productivity in the derivation of numeric nutrient criteria for streams and rivers (USEPA 2000). This
encouragement for the use of algae in water quality standards and criteria development is reflected in EPA
biological assessment programs. The Rapid Bioassessment Protocol (Barbour et al. 1999) has a chapter on algal
methods, which are recommended for use because of algal sensitivity to stressors, especially nutrients, and
importance to food webs. The chapter covers natural substrate and artificial substrate methods for diatom and
non-diatom algae, lab processing, indicator development, and a rapid visual survey method. Similar algal methods
and indicators were developed and included in the wadeable stream algal protocols for both the Environmental
Monitoring and Assessment Program (EMAP) and the National Aquatic Resource Surveys (NARS) (Appendix I)
(USEPA 2009).
In addition to EPA, other agencies also actively assess algae. For example, diatoms and non-diatom algae are a
central component of the U.S. Geological Survey (USGS) National Water Quality Assessment (NAWQA) stream
assessment protocol (Table 1) (Moulton et al. 2002), which is used by several state monitoring programs. The
NARS and NAWQA protocols are both quantitative multiple habitat or transect methods that measure both
benthic algal biomass as chlorophyll a and algal taxonomic composition. Quantitative multihabitat methods sample
known areas of multiple habitats, thereby sampling a broad range of species, and transect methods are
quantitative methods that also sample multiple habitats. Quantitative methods are important because they
capture more taxa and increase precision, representativeness, and replicability, compared to qualitative methods.
See Table 2 for a list of different types of quantitative methods frequently used by state and federal agencies. This
information is translated using algal metrics or multimetrics. In the case of the recent NARS National Rivers and
Streams Assessment, EPA calculated an algal multimetric index, or MMI (USEPA 2013b), which was used to
interpret the biological condition of the nation's streams and rivers. In addition to quantitative methods, state and
federal agencies use qualitative methods, which identify the taxa present, but not actual abundances, and without
considering the sampled area (see Table 2 for a list of different types of qualitative methods frequently
recommended or used by state and federal agencies).
Table 1. Federal agency programs encouraging or applying use of algae in stream regulation or monitoring.
Federal Agency Program
Application
Citation
USEPA/OST
Biocriteria Development
States encouraged to use multiple assemblages
including algae for biocriteria in streams
(USEPA 2011, 2013a)
USEPA/OST
Nutrient Criteria Development
States encouraged to use primary producer
measures to derive nutrient criteria for
streams and rivers
(USEPA 2000)
5

-------
USEPA
Biological Assessment
Rapid Bioassessment Protocol for streams
includes algal methods chapter for diatoms (Barbour et al. 1999)
and non-diatoms
USEPA ORD/OWOW
EMAPand NARS
EPA national monitoring programs employ
methods to collect algae in streams; NARS
USEPA2009, 2013b
developed algal multimetric index to evaluate
biological condition of nation's streams
USGS
NAWQA Program
USGS national monitoring program employs
algal sampling methods for diatoms and non- (Moulton et al. 2002)
diatoms
Table 2. Quantitative and qualitative methods frequently used to sample and assess algae.
Quantitative Methods
Method
Description of method
Quantitative multihabitat
method
Scraping algae from known areas of several different habitats likely to support
algae; samples may be held separate or combined.
Quantitative richest targeted
habitat method
Scraping algae from known area of the habitat most likely to support the most
algae at any site (e.g. rocks).
Passive periphytometer
method
Deploying frame of glass slides upon which algae settle and grow.
Visual transect point-
intercept method
Estimating percent cover at several locations along cross-sectional transects;
measures at each point generally include some combination of percent algal
cover or abundance, filament length, and periphyton thickness. Additional
measures may include color, condition, and algal identification for soft algae.
Algal growth potential
Taking filtered stream water and measuring the growth of a single laboratory
algal species in that water.
Qualitative Methods
Method
Description of method
Qualitative multihabitat
composite sample method
Taking scrapings of periphyton algal material from multiple habitats and
combining them for identification.
Qualitative targeted habitat
General scrapings from specific targeted habitats.
Qualitative soft-algal method
Gathering a sample of representative soft algae for identification.
Qualitative point-transect
composite method
Collect general scrapings from multiple substrates at points across multiple
cross-section transects.
Twenty-three states were identified as having water quality programs that evaluate algae and a subset (11
states) of those were identified as having substantial program integration, including incorporation of algal
measures into criteria exploration or development (USEPA 2002). The following discussion focuses on these 11
states first, providing a detailed review of each of them, followed by a general synthesis. A detailed table
summarizing these state methods and applications is provided in Appendix I, along with references, for algal
indices or other tools developed by states.
6

-------
CALIFORNIA
California has developed standard methods for collecting diatom and non-diatom (soft algae) samples from
streams for the purposes of biological assessment and numeric nutrient criteria or endpoint development
(Fetscher et al. 2010). They use a mix of quantitative multihabitat methods for diatoms and non-diatoms,
qualitative soft-algal methods, and visual transect point-intercept methods for percent benthic cover. The State
estimates benthic biomass as chlorophyll a and AFDM, percent cover of algae, and identifies diatoms and non-
diatoms in order to interpret that assemblage composition information with diatom/non-diatom multimetric
index/indices.
In addition to the Statewide efforts, several regional water boards in California have explored development of
diatom assemblage composition indices (multimetric and Observed/Expected [O/E] type models) for use in
assessment. These have been explored for the San Diego, Lahontan, and Central Coast regions as well as the
eastern Sierra Nevada mountains (Appendix I).
CONNECTICUT
Connecticut has an algal sampling program. They use USGS NAWQA methods, which consist of a quantitative
richest targeted habitat sample to estimate algal biomass (chlorophyll a and AFDM) and quantitative diatom
assemblage composition, as well as a qualitative targeted habitat sample from depositional habitats and a
qualitative multihabitat composite sample. From the quantitative samples, the State estimates algal biomass and is
developing an algal multimetric index. They have also used the raw diatom data to conduct stressor-response
analyses to explore nutrient thresholds using TITAN analysis, change-point analysis, and boosted regression trees
(Smucker et al. 2013).
FLORIDA
Florida has been using algal sampling for several years for biological assessment and criteria development.
They measure benthic biomass (chlorophyll a) using quantitative multihabitat methods, water column algal
biomass using quantitative volumetric filtration, percent benthic cover using a visual rapid periphyton survey of
cover, thickness, and filament length based on the rapid bioassessment method (Barbour et al. 1999). In addition,
the State measures benthic algal assemblage composition using a quantitative multihabitat method, a quantitative
passive periphytometer method, and algal growth potential method. The State uses the water column biomass,
percent cover, and species dominance in assessment as part of a combined criteria approach, explored
development of an algal multimetric index for assessment, and used stressor-response relationships between
nutrients and visual cover and biomass for criteria development.
7

-------
IDAHO
Idaho samples benthic diatom assemblage composition using a quantitative richest targeted habitat method.
They use these data to develop an algal multimetric index for potential use in biological assessment, and are
conducting stressor-response analyses of nutrients versus diatom data for nutrient criteria exploration.
KENTUCKY
Kentucky has had an algal based sampling program for many years as well. This State samples benthic biomass
(chlorophyll a) and benthic diatom assemblage composition using a quantitative richest targeted habitat method.
They also measure percent benthic cover using a visual transect point-intercept method, and the State has
separate qualitative targeted habitat methods and qualitative multihabitat composite sample methods to measure
or detect additional algal species richness. The State has developed an algal multimetric index, which is used in
biological assessment, and they are exploring stressor-response relationships between nutrients and algal data for
use in criteria development.
MAINE
Maine has recently developed a substantial algal sampling program. They sample benthic biomass (chlorophyll
a) and benthic algal assemblage composition using a quantitative richest targeted habitat sampling method as well
as a passive periphytometer method. They also measure percent benthic cover using a visual transect point-
intercept method. The State has developed total phosphorus (TP) and total nitrogen (TN) optima for algal taxa to
develop tolerance values, used to construct some Maine-specific algal metrics. These were combined with other
general algal metrics and are used to assign sites to aquatic life use tiers as part of their assessment program. They
are also developing N and P inference models (inferring the nutrient conditions based on a weighted average of
algal nutrient optima for taxa present) and used diatom data in stressor-response models to develop proposed
numeric nutrient criteria.
MINNESOTA
Minnesota has developed and applied algal measures in setting criteria for rivers and streams. They measure
benthic biomass (chlorophyll a) and benthic algal assemblage composition using the USGS NAWQA methods,
primarily the quantitative richest targeted habitat sample method. They also measure water column biomass
(chlorophyll a) using a standard quantitative volumetric filtration method. The State has used standard algal
metrics for interpreting assemblage data and explored the use of these as response measures in stressor-response
models for numeric nutrient criteria development.
8

-------
MONTANA
Montana has long had a substantial algal sampling program. They sample benthic biomass (chlorophyll a)
using a quantitative richest targeted habitat sampling method. They also measure percent benthic cover using a
visual transect point-intercept method. The State also has qualitative algal assemblage composition methods using
a qualitative multihabitat composite sample method from non-wadeables and a qualitative point-transect
composite method for wadeables. The State uses a variety of diatom metrics including state-specific diversity,
siltation, and pollution metrics. The indices are used in assessment and were used, along with biomass measures
and visual cover measures, in stressor-response models to support numeric nutrient criteria development.
NEW JERSEY
New Jersey samples benthic biomass (chlorophyll a) and benthic algal assemblage composition using a
quantitative richest targeted habitat sampling method. The State has developed TP and TN inference models
(inferring the nutrient conditions based on a weighted average of algal nutrient optima for taxa present) and
trophic diatom indices that are rescaled inference model values (0-100). The State uses the inference models and
a diatom biological condition gradient (BCG) (Hausmann et al. 2016) to develop assessment tools and to support
numeric nutrient criteria development. Rhode Island
Rhode Island has recently developed algal methods and analysis tools. They sample benthic biomass
(chlorophyll a) and benthic algal assemblage composition using a quantitative richest targeted habitat sampling
method as well as a passive periphytometer method. They also measure percent benthic cover using a visual
transect point-intercept method. The State is using the raw diatom data to conduct stressor-response analyses to
explore nutrient thresholds using TITAN and change-point analyses.
WEST VIRGINIA
Similar to many of the other states, West Virginia samples benthic biomass (chlorophyll a) and benthic algal
assemblage composition using a quantitative richest targeted habitat sampling method. They also measure
percent benthic cover using a visual transect point-intercept method. The State uses standard algal metrics as
interpretive tools and is exploring the use of diatoms in numeric nutrient criteria development for aquatic life uses.
In addition, a percent cover of 40% is used as a numeric translator of the narrative recreational use standard for a
single transect.
EPA NATIONAL AQUATIC RESOURCES SURVEY (NARS) AND USGS NATIONAL WATER-QUALITY
ASSESSMENT (NAWQA) PROGRAM
The NARS program at EPA and the NAWQA program at NASA both sample algal biomass (as chlorophyll a and
AFDM) using quantitative methods. For benthic algal assemblage composition, EPA NARS uses a quantitative
richest targeted habitat method at multiple fixed transect locations. USGS NAWQA uses a quantitative richest
9

-------
targeted habitat method as well. USGS NAWQA collects additional qualitative depositional and multihabitat
samples, the former from pools and the latter from a variety of habitats, to search for additional algal taxa.
In addition to the states described above, twelve other states reported periphyton sampling to USEPA (USEPA
2002), but no further information could be identified elaborating on their methods or applications. These states
are identified in Appendix I.
STATE SUMMARY
All of the states use quantitative active sampling methods that sample natural substrates in a manner that
provides replicable estimates of diversity, abundance, and biomass. Algal biomass is typically estimated with
chlorophyll a, and algal assemblage composition measured using microscopic identification of algae, most often to
the species level. Most states sample from known areas of habitat believed to provide the best substrate for algal
diversity and biomass (richest targeted habitat), which are often cobble in steep streams, and wood, macrophytes,
or depositional materials (sand/silt) in low gradient systems. Most composite several samples from multiple
substrates and/or transects into one sample to represent the site. Four states (FL, ME, NJ, and Rl) also use passive
periphytometer samplers, which are glass slides deployed in streams upon which a subset of stream algae grow.
Eight states also conduct visual algal cover surveys, which consist of estimates or direct measurement of the extent
and thickness of visual algal cover, as well as the length of any filaments. Most of these states use points along
single or multiple transect for this measure. Kentucky also identifies large green and red algae as part of their
visual method.
In terms of assemblage composition indicators, 8 of 11 states use algal metrics, which are measures of the
diversity/richness, composition, traits, and autecology of the resident algae. Autecological measures include
tolerance/sensitivity metrics to different pollutants including nutrients, pH, and oxygen. Most states (8) are
measuring both diatoms and non-diatom algae, the others focus on diatoms alone. CT and Rl use multivariate tools
to measure thresholds in algal assemblage response to nutrient gradients for investigating nutrient concentrations
that impact streams, but not for criteria development. New Jersey has developed inference models using algae.
These are models that use taxon-specific nutrient optima to estimate the average nutrient concentrations at sites.
New Jersey also uses diatom assemblage composition in the context of a Biological Condition Gradient (BCG)
(Hausmann et a I, 2016) to assess diatom community condition (pristine to severely disturbed).
Eight of the states are using or have used algal data in nutrient criteria development efforts. Most are using
these data in stressor-response type analyses, looking for thresholds in ecological responses or interpolating values
associated with desired biological conditions, most often based on reference condition. West Virginia is the only
state identified that is currently using thresholds in visual cover as a recreational use criterion. The threshold for
that was identified using a stressor-response model of user-perception survey data tied to their narrative aesthetic
10

-------
criterion (Responsive Management 2012). Montana has pursued development of user perception based algal cover
endpoints in support of their nutrient criteria development efforts (Suplee et al. 2009).
EPA compiled a summary of state bioassessment and biocriteria programs in 2002 (USEPA 2002). That study
documented an additional 12 states that have reported sampling algae, including periphyton, in their assessment
programs, but not explicitly in criteria or criteria development (Appendix I - Summary table of U.S. state algal
indicator endpoints, methods, interpretive tools, and use in criteria development and/or assessment.). Like the 11
states referenced above, these 12 primarily use quantitative active sampling of richest targeted habitat. Five of
these also reportedly used passive periphytometer samplers and two (NM and SD) also collected a qualitative
multihabitat sample. Most of these other states (8) focused on the entire algal assemblage, whereas the others
focused on diatoms. None of these states reported any interpretive tools as part of this survey, so the extent to
which these states still sample algae or have developed interpretive tools for use in criteria development is
unknown. More recently, algal indicators have also been developed in Alaska for local application in urban streams
and the Cook Inlet region (Rinella and Bogan 2007, Rinella and Bogan 2010).
In summary, 23 states were found in this synthesis to be evaluating algae routinely and a subset of those (11)
are known to have developed interpretive tools or to have incorporated analysis of algal responses into nutrient
criteria or biocriteria development. Major impediments to greater development likely include unfamiliarity,
taxonomic expertise, and financial constraints. Algae are not as commonly applied in monitoring in this country
and are an assemblage that may be less familiar than macroinvertebrates or fish, at least methodologically, to
many state resource scientists. This can easily be overcome by disseminating the many method documents that
exist and conducting training workshops. Taxonomic expertise is a limit because there are few labs and experts
capable of identifying algal taxa, especially diatoms, to the species level. In addition, taxonomic consistency among
labs is an issue, which may also fuel resistance (Besse-Lototskaya et al. 2011; Kahlert et al. 2012). These hurdles
could be overcome by encouraging additional training of taxonomic experts, support for development of molecular
identification techniques, and encouragement of taxonomic resolution among active labs, something that the
European Union (EU) has been actively pursuing through the Water Framework Directive (Besse-Lototskaya et al.
2011; Besse-Lototskaya et al. 2006; Kahlert et al. 2012). Financial constraints are related to taxonomic expertise,
since states that are already constrained by available funding, may be reticent to add an assemblage that requires
additional sampling and taxonomic identification. The resolution for this constraint would be more investment in
state monitoring programs for algal sampling and identification. The EPA is working to alleviate many of these
impediments by providing monitoring support, training states in methods via NARS, working on a common
taxonomy, and supporting research into molecular techniques for algal identification.
11

-------
RESEARCH ON ALGAL ASSESSMENT AND INDICATOR USE IN STREAMS
This section will introduce independent (non-state and non-federal) studies and research conducted on algal
indicators (e.g., diatom indices), in academia and the peer-reviewed literature, both in the U.S. and outside the
U.S. More than 250 peer-reviewed manuscripts related to the use of algae in evaluating stream condition were
reviewed. This information was organized into several general thematic areas, which are described here. These
themes include:
	Geographic application
	Interpretive tools
	Indicator development
	Indicator comparisons
	Specific pollutant source application
	Novel insights from research related to chemical effects, habitat effects, variability, indicator analysis,
and methods.
GEOGRAPHIC APPLICATION
Specific algal research was identified from at least 11 different unique states (CA, CT, ID, KY, ME, Ml, NJ, NY,
OH, OR, TN) (see Table 3 for state-specific studies). These include studies that involved specific analyses that could
support state numeric nutrient criteria development, for example, threshold analyses of diatom response in CT
(Smucker et al. 2013) and papers describing development of the algal indices included in the state review above,
for example in California (Fetscher et al. 2014a), Idaho (Fore and Grafe 2002a), Maine (Danielson et al. 2012), and
New Jersey (Ponader et al. 2007). As a whole, this research highlights the sensitivity of diatom and non-diatom
algae to a variety of stressors (especially nutrients, sediment and acid mine drainage), their value as assessment
tools across multiple states, and the variety of indicator options to use with algae. The last insight includes
development of nutrient optima models with weighted averaging, predictive models (site specific metric
predictions based on geomorphic predictors), multimetric models, percent model affinity models (comparing test
sites to reference site composition), and ecosystem level measures of biomass and productivity.
Table 3. Breadth of states within which algal indicator research is being conducted.	
State
Research
Citation
CA
Development and comparison of diatom and non-diatom algal
indicators; comparison of response of ecosystem measures and
community level indicators to nutrient pollution
(Fetscher et al. 2014a;
Nelson et al. 2013)
CT
Application of regression tree and threshold analysis with algal
metrics and biomass to develop numeric criteria
(Smucker et al. 2013)
ID
Development of algal indices for wadeable and non-wadeable
streams using traditional multimetric and predictive multimetric
models
(Cao et al. 2007; Fore and
Grafe 2002b)
12

-------
KY
Comparison of biomass responses of algae to nutrient gradients
with criteria development implications
(Stevenson et al. 2006)

Development of nutrient optima for algal species and state-

ME
specific algal metrics for use in assessing streams using those
(Danielson et al. 2011;
optima; Development of a model to rate sites into aquatic life
use tiers using algal metrics
Danielson et al. 2012)
Ml
Comparison of biomass responses of algae to nutrient gradients
with criteria development implications
(Stevenson et al. 2006)

Development of weighted average nutrient optima for algal
(Ponader et al. 2007;
Ponader et al. 2008)
NJ
species and their use in state specific algal indicator creation for
assessment and criteria derivation
NY
Development of algal index based on comparison of algal
assemblage composition to that at reference sites
(Passy and Bode 2004)

Development of a diatom index based on comparison to

OH
reference site composition and a diatom multimetric index to
(Smucker and Vis 2009,
assess agriculture and acid mine drainage effects; Study of
influence of acid-mine drainage on algal succession
2013; Zalack etal. 2010)
OR
Investigation of land use effects on diatom composition
(Weilhoefer and Pan 2006b)

Comparative study of non-point source pollution on diatom

TN
assemblage composition, primary production, and excessive
algal growth
(Lebkuecher et al. 2011)
There were also a number of regional studies (Table 4) that analyzed algal response in streams across broad
regions including in the eastern U.S., Appalachians, Midwest and the Western U.S. (Black et al. 2011; Carlisle et al.
2008; Charles et al. 2006; Gillett et al. 2011; Griffith et al. 2002; Hill et al. 2000; Hill et al. 2001; Justus et al. 2010;
Stevenson et al. 2008b; Walker and Pan 2006; Wang et al. 2005), as well as for large rivers (Kireta et al. 2012a;
Kireta et al. 2012b; Reavie et al. 2010). In the same vein, there have been several studies published using the large
national NAWQA periphyton dataset (Porter et al. 2008; Potapova and Charles 2007; Potapova and Charles 2002;
Potapova et al. 2004). These broad regional and national studies identified strong regional controls of pH/alkalinity
and hardness on diatom assemblage structure, helped develop algal sampling methods and indicators, and
reinforced the sensitivity of algae and algal indicators to a variety of stressors, especially nutrients.
Table 4. U.S. areas within which broad regional algal indicator research is being conducted.
Eastern U.S.
Reearth
Exploration of major environmental controls
on diatom distributions at multiple scales
Citation

(Charles et al. 2006)
Mid - Appalachians
Development of multimetric indices using algal
richness, composition, and biomass measures;
Comparison of genus and species level
taxonomy on indicator response to stressors;
Comparison of diatom to other assemblage
response to land use
(Carlisle et al. 2008; Hill
et al. 2000; Hill et al.
2001)
Interior Plateau Ecoregion (KY, TN,
IN, and OH)
Diatom multimetric development and testing (Wang et al. 2005)
Mississippi, Missouri, and Ohio
Rivers
Evaluation of sampling methods for algae and
response of metrics to stressors in large rivers;
Development of diatom based indicators for
(Kireta et al. 2012a;
Kireta et al. 2012b;
Lane et al. 2007; Reavie
13

-------

large rivers; Comparison of benthicvs.
et al. 2010; Stevenson

planktonic algal indicators in large rivers
et al. 2006)

Comparative study of algal indicators to other

| Ozark Region
assemblages showing highest sensitivity of
diatoms to nutrients
(Justus et al. 2010)
| Rockies
Analysis of algal metric response to pollutant
gradients
(Griffith et al. 2002)

Development of novel diatom metrics including

| Western U.S.
those based on algal weighted average optima
for a variety of stressors and percent live
(Black et al. 2011;
Gillett et al. 2011;
Stevenson et al. 2008b)
diatoms; exploration of the relative importance
of nutrients, land use, and habitat on diatoms
and development of nutrient thresholds;
Europe has a more extensive application of algal measures than the U.S., particularly in stream condition
assessment (Birk et al. 2012). This is likely a function of the Water Framework Directive emphasis on biological
monitoring of the entire community, and also the long use of algae for assessment of water quality in Europe
starting in the early 1900s (Stevenson et al. 2010). Eighteen European countries have algal assessment methods,
and the EU collaboration has supported development of indices and software to calculate a wide number of these
indices (e.g. OMNIDIA software). Countries with active research, assessment, and indices for algae include: Austria,
Belgium, Bulgaria, Finland, France, Germany, Hungary, Iceland, Italy, Latvia, Luxembourg, Norway, Poland,
Portugal, Spain, Switzerland, Turkey, and the United Kingdom (Table 5). In addition to this work, the EU has put
substantial effort into regional syntheses, which have focused on harmonizing assessments across countries,
resolving issues related to reference condition, and taxonomic comparability (Almeida et al. 2014; Besse-
Lototskaya et al. 2011; Besse-Lototskaya et al. 2006; Birk et al. 2012; Borics et al. 2007; Fisher et al. 2010; Hering et
al. 2006; Johnson et al. 2006; Kahlert et al. 2012; Kermarrec et al. 2014; Kloster et al. 2014). The EU assessment
(2011) found that the algal index score varied due to a lack of standardized methods, taxonomy, and counting
consistency and are working to rectify this (Besse-Lototskaya et al. 2011). There are also a variety of indices used
across Europe, and the EU is working to harmonize the metrics and indices using reference standardization
(Almeida et al. 2014).
Many other countries have also embraced the use of algae in assessment (Table 5). In eastern Canada,
extensive work has been done, especially focused on pollutant source impacts, but also on some of the first
nutrient inference modeling for streams (Lavoie et al. 2006a; Lavoie et al. 2006b; Lavoie et al. 2014; Mazor et al.
2006; Winter and Duthie 2000; Wunsam et al. 2002). Additionally, one study in the Fraser River in western Canada
compares multivariate assessment indices (Mazor et al. 2006). Algae have also been used in Mexico (Vazquez et al.
2011). No assessment studies in Central America were identified, although there are several research studies using
algae in Costa Rica (e.g., Pringle and Hamazaki 1997). South American countries are represented by several
assessment related studies using algae in Brazil and Argentina (Gomez and Licursi 2001; Lobo et al. 2004a; Lobo et
al. 2004b; Salomoni et al. 2006). In Asia, diatoms are seeing increasing use in assessment, and several papers were
14

-------
identified related to algal assessment methods or applications in India, Iran, Japan, and especially China (Table 5).
Similarly, in Africa, there have been several applications, including in Ethiopia, Kenya, Eastern Africa, and especially
South Africa (Table 5). New Zealand has a long history of using algae in assessment and has developed
comprehensive sampling protocols, indices, and even nutrient thresholds based on algal responses in streams
(Biggs 2000; Biggs and Kilroy 2000; Schowe and Harding 2014). In addition, research from Australia also indicates
the application of algal assessment measures there (Dela-Cruz et al. 2006).
Table 5. Sample breadth of countries outside the U.S. within which algal indicators are applied and/or research
is being conducted.
Country/Region


(Rott and Schneider 2014)
Latvia
(Springe et al. 2006)
(Triest et al. 2001)
Luxembourg
(Gevrey et al. 2004)


(Passy 2007; Rott and Schneider
(Passy 2007)
Norway
2014; Schneider and Lindstrom
2011)
(Eloranta and Soininen 2002;


Mykra et al. 2012; Raunio and
Poland
(Picinska-Faltynowicz 2009; Szulc
Soininen 2007; Soininen and
and Szulc 2013)
Niemela 2002)


(Berthon et al. 2011; Blanco et al.
2012)

(Adams et al. 2014; Feio et al.
Portugal
2007, 2009; Mendes et al. 2012;

Mendes et al. 2014)
(Kloster et al. 2014)
Spain
(Delgadoetal. 2010, 2012;
Douterelo et al. 2004)
(B-Beres et al. 2014; Stenger-


Kovacs et al. 2013; Stenger-
Switzerland
(Solak and Acs 2011)
Kovacs et al. 2014)


(Gudmundsdottir et al. 2013)
Turkey
(Solak and Acs 2011)
(DelTuomo and Torrisi 2011; Gallo


et al. 2013; Lai et al. 2014; Torrisi
United Kingdom
(Solak and Acs 2011; Vinten et al.
and Dell'Uomo 2006; Torrisi et al.
2011)
2008)



(Lavoie et al. 2006a; Lavoie et al.
2006b; Lavoie et al. 2014; Mazor
et al. 2006; Winter and Duthie
2000; Wunsam et al. 2002)

(Gomez and Licursi 2001; Lobo et
Brazil
al. 2004a; Lobo et al. 2004b;
Argentina
Salomoni et al. 2006; Salomoni et
al. 2011)
(Vazquez et al. 2011)
	
(Juttner et al. 2003; Karthick et al.
2010)
Japan
(Toda et al. 2002)


(Liu et al. 2013; Pignata et al.
(Atazadeh et al. 2007)
China
2013; Tang et al. 2006; Wang et
al. 2009; Wu etal. 2012)

(Beyene et al. 2009; Beyene et al.
2014)
Eastern Africa
(Bellinger et al. 2006)
15

-------
(Beyene et al. 2009; Beyene et al.
8nya 2014; Triest et al. 2012)
(de la Rey et al. 2008a; de la Rey
, . et al. 2004; de la Rey et al. 2008b;
South Africa ,, , T ,
Harding et al. 2005; Taylor et al.
2007)
Oceania
Australia (Dela-Cruz et al. 2006)
New Zealand (Biggs 2000; Biggs and Kilroy
2000; Schowe and Harding 2014)
INTERPRETIVE TOOLS
As described earlier, algae have been used in biological monitoring for a long time (Stevenson 2014; Stevenson
et al. 2010; Stevenson and Smol 2003). Since their earliest application in saprobien indices in Germany, algal
assemblage composition data have frequently been interpreted using metrics and indices that combine
information on the diversity of species, their traits, and what is known about individual species ecology
(autecology) to evaluate water quality. This section splits interpretive tools into those that use taxonomic
composition and those that use biomass.
COMPOSITION BASED TOOLS
The following tools (Table 6) employ benthic taxonomic presence/absence and abundance data collected
using standardized methods to develop indicators that are used as measures of condition. These include
multimetric indices, O/E or taxonomic completeness indices.
MULTIMETRIC INDICES
Multimetric indices (MMI) are by far the most common tool used around the world (Table 6). These consist of
metrics reflecting diversity (number of taxa), composition, traits (e.g., growth form and motility) and autecological
properties of individual taxa (e.g., tolerance/sensitivity to different pollutants). Individual metrics are selected
based on a number of factors (e.g., responsiveness to stressors, redundancy, precision, range, etc., (Barbour et al.
1999) and scored using reference sites or ranges of conditions, standardized, and then combined into an overall
MMI. Representative values are then used as boundaries for condition assessment. A proliferation of regional
indices has developed around the world and while indices vary in their portability to different areas, some indices
and especially component metrics, exhibit broad applicability (Pignata et al. 2013).
Table 6. Methods to interpret algal assemblage composition data used in biological condition assessment,
monitoring, and criteria development
Index
Citation
Multimetric
(Alvarez-Bianco et al. 2013; Atazadeh et al. 2007; B-Beres et al. 2014;
Bellinger et al. 2006; Blanco et al. 2012; Cao et al. 2007; de la Rey et al.
2008a; de la Rey et al. 2004; Delgado et al. 2010, 2012; Delluomo and
Torrisi 2011; Eloranta and Soininen 2002; Fetscher et al. 2014a; Fore and
16

-------
Grafe 2002b; Gallo et al. 2013; Gomez and Licursi 2001; Harding et al.
2005; Hill et al. 2000; Kelly et al. 2008; Lai et al. 2014; Lebkuecher et al.
2011; Lobo et al. 2004a; Mendes et al. 2012; Picinska-Faltynowicz 2009;
Pignata et al. 2013; Potapova et al. 2004; Rott et al. 2003; Salomoni et al.
2011; Schneider and Lindstrom 2011; Schowe and Harding 2014;
Smucker and Vis 2009, 2011; Solak and Acs 2011; Stevenson et al. 2008b;
Tan et al. 2013; Tang et al. 2006; Taylor et al. 2007; Torrisi and Dell'Uomo
2006; Triest et al. 2001; Triest et al. 2012; Wang et al. 2005; Wu et al.
2012; Zalack etal. 2010)
O/E Type and Multivariate
(Carlisle et al. 2008; Feio et al. 2007, 2009; Kelly et al. 2009; Lavoie et al.
2006a; Lavoie et al. 2006b; Lavoie et al. 2014; Mazor et al. 2006; Mendes
etal. 2014)
Inference Models
(Alvarez-Bianco et al. 2013; Kireta et al. 2012b; Ponader et al. 2007;
Ponader et al. 2008; Soininen and Niemela 2002; Wang et al. 2009;
Winter and Duthie 2000)
Biological Condition Gradient (BCG) (Danielson et al. 2012)
Multivariate Analysis
(Beyene et al. 2009; Beyene et al. 2014; Charles et al. 2006; Fisher et al.
2010; Griffith et al. 2002; Korhonen et al. 2013; Lavoie et al. 2006a;
Lavoie et al. 2006b; Lavoie et al. 2014; Pan et al. 2006; Potapova and
Charles 2002; Salomoni et al. 2006; Smucker and Vis 2009; Stancheva et
al. 2011; Walker and Pan 2006; Weilhoefer and Pan 2006b; Winter and
Duthie 2000)
TAXONOMIC COMPLETENESS INDICES
In terms of interpretive index type tools, taxonomic completeness indices, including Observed/Expected (O/E)
type, some multivariate models, and similarity indices were the next most commonly used to MMI (Table 6). In
contrast to multiple metrics, taxonomic completeness models compare the completeness of observed taxa
richness to that expected under least disturbed conditions. The most common tool in this group is the O/E index.
With an O/E index, one compares the expected taxa richness (E) modeled based on biogeographic predictors to
that observed (O) at the site. A deviation from 1 indicates impact. Multivariate models (e.g., Benthic Assessment of
SedimenT, BEAST) are similar to O/E type models as they measure how similar the species composition at a test
site is to the population of regional reference sites. A significant departure from the natural variability in species
composition is interpreted as an impact. In a similar vein, percent model affinity indices are simple multivariate
models, and are cited in Table 6 in the multimetric category. Percent model affinity indices compare the similarity
of composition of any test site to the average composition expected in reference sites but not using complex
multivariate statistics but rather a similarity index. New York uses percent model affinity indices for
macroinvertebrates and algae (Passy and Bode 2004).
INFERENCE MODELS
In addition to these index models, a number of studies have developed nutrient inference models (Table 6).
Nutrient inference models use the observation that individual taxa have nutrient conditions under which they
17

-------
achieve their highest abundances or nutrient optima. These optima exist as a result of competitive differences for
nutrients among taxa. Once these optima are known, then the taxa present at a site can be used to infer the likely
average nutrient conditions based on averaging the taxa nutrient optima often weighted by relative abundances.
This is arguably a better potential measure of the true average nutrient conditions since these taxa integrate
nutrient concentrations over a longer time period than a single or few water quality grab samples. The trophic
diatom index developed for use in New Jersey is an inference model re-scaled from 0-100 (Ponader et al. 2007;
Ponader et al. 2008).
3) MODELS
Maine has developed a biological condition gradient (BCG) type model for use with algae akin to the one they
have developed for use with macroinvertebrates (Table 6) (Danielson et al. 2012). As opposed to a single aquatic
life use, Maine has multiple aquatic life use classes organized along a gradient in biological condition (tiered
aquatic life uses) used to protect high quality waters and to improve waters incrementally (USEPA 2011). The algal
BCG model was constructed by asking experienced algal and water quality experts to classify sites into the
appropriate aquatic life use tiers using the state narrative tier descriptions and algal metric data for each site.
These decisions were then automated using discriminant function analysis so future sites can be classified based
on their algal metric values alone.
MULTIVARIATE ANALYSIS
A number of studies used traditional multivariate analyses (including canonical correspondence analysis, non-
metric multidimensional scaling, two-way indicator species analysis, and classification and regression trees) to
analyze and interpret algal assessment data (Table 6) (Beyene et al. 2009; Beyene et al. 2014; Charles et al. 2006;
Fisher et al. 2010; Griffith et al. 2002; Korhonen et al. 2013; Lavoie et al. 2006a; Lavoie et al. 2006b; Lavoie et al.
2014; Pan et al. 2006; Potapova and Charles 2002; Salomoni et al. 2006; Smucker and Vis 2009; Stancheva et al.
2011; Walker and Pan 2006; Weilhoefer and Pan 2006b; Winter and Duthie 2000). These techniques are commonly
used to interpret ecological community data and to relate environmental gradients to patterns in species
occurrence and abundance. They are common interpretive tools, for analytical purposes, and frequently used to
inform the development of the most common multimetric and taxonomic completeness indices.
BIOMASS
A frequently used algal measure in streams is biomass, which is measured by chlorophyll a, ash-free dry mass
or biovolume methods (Barbour et al. 1999, USEPA 2000). The most commonly used of the biomass indicators is
chlorophyll a, one of the photosynthetic pigments found in algae (USEPA 2000). It has a long history of application
for estimating algal biomass in aquatic systems, despite the fact that chlorophyll cell content among algae varies
due to physiological and genetic factors. In streams, both water column (sestonic) as well as bottom (benthic)
measures of chlorophyll are used (USEPA 2000). For the former, water column samples of suspended algae are
18

-------
filtered onto glass fiber filters and chlorophyll extracted to estimate volumetric chlorophyll a biomass. For the
latter, a known area of substrate is scraped and the resulting periphyton filtered onto glass fiber filters and
chlorophyll extracted to estimate areal chlorophyll a biomass.
Ash free dry mass is another measure used (USEPA 2000). Ash free dry mass is measured similarly, from the
water column or benthic samples. Instead of chlorophyll a extraction, to estimate ash free dry mass, the water
sample or benthic sample is filtered onto pre-weighed glass fiber filters and the organic content estimated by
subtracting the mass after combustion at 500 degree C (to remove all organic matter) from the mass after drying
the sample (to remove water). This difference is the ash free dry mass. It is a less accurate measure of algal
biomass since it also contains non-algal detritus, microbes, small invertebrates, etc. that all contribute to ash free
dry mass.
Biovolume is often used to estimate algal biomass (Barbour et al. 1999). For this estimate, the approximate
dimensions of living cells are taken using microscopy and geometric equations used to estimate the volumetric
mass of organic matter. Biomass can be estimated using published cell volume to biomass conversions.
INDICATOR DEVELOPMENT
Developing indicators, especially multimetric indicators, relies on developing metrics using trait and
autecological information about different taxa (e.g., growth forms, motility, and pollutant tolerance/sensitivity).
Much of the information on the former two (growth forms/motility) is available in ecological and taxonomic texts,
but much of the latter (pollutant sensitivity) is developed using inference models. Weighted-averaging partial least
squares models are among the more common technical approaches to do this, and these models have been
applied to develop such information for algal taxa (Alvarez-Bianco et al. 2013; Danielson et al. 2011; Dela-Cruz et
al. 2006; Kireta et al. 2012b; Potapova et al. 2004; Stevenson et al. 2008b). These weighted-average models help
identify optima, which can then be relativized to infer sensitivity or tolerance. These models perform best when
pollutant responses are unimodal, an assumption that is not always met and needs to be considered in developing
optima (Potapova et al. 2004). Simple regression models of abundance to pollutant gradients have also been used
to infer sensitivity/tolerance (Stevenson et al. 2008b).
Once identified using approaches like those described above, metrics are generally constructed to be sensitive
to pollutants, especially nutrients. Some very unique metrics have been developed and have been found to be
especially sensitive on a national scale. For example, the relative abundance of nitrogen fixers was inversely
proportional to nitrogen concentration, and high dissolved oxygen taxa were inversely proportional to nitrogen to
phosphorus (N:P) ratio across the U.S. using the NAWQA dataset (Porter et al. 2008). Since diatoms are identified
by their silicate cases (or frustules) that do not necessarily reflect live individuals, another example explored the
use of percent live diatom metrics and found them of mixed performance (Gillett et al. 2011). It is quite likely that
novel metrics will continue to be developed and prove valuable in various applications.
19

-------
Recent research has compared and contrasted diatom only and hybrid diatom and non-diatom algal models.
These studies have found excellent performance from both diatom only, hybrid, and even non-diatom only models
(Fetscher et al. 2014a; Stancheva et al. 2012). There appears to be a tendency to incorporate more non-diatom
taxa into assessment models, especially since as many of the nuisance stream taxa are not diatoms.
Metrics have been used in assessment models alone or as multimetrics. In some instances, single metric indices
have been found to be sufficient as indicators (Ponader et al. 2007; Schowe and Harding 2014), in other cases
MMIs have been found to be superior to single metrics (Delgado et al. 2010). Multimetrics are far more common,
but testing is routinely performed to evaluate the performance of any of these model options.
INDICATOR COMPARISONS
In many cases, indicators and even metrics and multimetrics have been found to be transferable to other
regions and to perform well. For example, European indices have been applied in many countries, facilitated by the
availability of software. European indices have been tested and found to work in China (Pignata et al. 2013; Tan et
al. 2013), India and Nepal (Juttner et al. 2003), Iran (Atazadeh et al. 2007), and Eastern and South Africa (Bellinger
et al. 2006; Taylor et al. 2007). Across Europe, comparison of indices has shown some promise as well (e.g.,
DelTuomo and Torrisi 2011; Rott et al. 2003). While models using default European data often work, the most
common observation has been that the regionally calibrated models generally outperform uncalibrated models,
emphasizing the need for local ecological information to optimize model performance (Danielson et al. 2012;
Delgado et al. 2010, 2012; Mendes et al. 2012; Potapova and Charles 2007; Rott et al. 2003).
Europe has undergone great analytical efforts to evaluate performance and comparability of metrics and
indices across countries. This effort has identified effects of methods and taxonomy on index scores as well as
differences in reference conditions that affect comparability of attainment boundaries and, therefore, general
condition assessments (Besse-Lototskaya et al. 2011; Besse-Lototskaya et al. 2006; Birk et al. 2012; Juttner et al.
2003). European scientists are working to harmonize such differences across entities to improve the comparability
of their stream algal assessments (Almeida et al. 2014).
In addition to comparison across regions, comparison across assemblages have also been conducted, for
example, assessments based on algae versus invertebrates, fish or macrophytes. There is no obvious a priori
reason to expect that all assemblages would respond the same to any given stressor and they often do not (Carlisle
et al. 2008), but all assemblages have been demonstrated to be successful indicators of environmental condition,
although some are more sensitive, and therefore, better than others (Johnson et al. 2006; Resh 2008; Zalack et al.
2010). Researchers have found algae to be more sensitive to nutrients than invertebrates (de la Rey et al. 2008a;
de la Rey et al. 2008b; Feio et al. 2007; Gallo et al. 2013; Gudmundsdottir et al. 2013; Hering et al. 2006; Justus et
al. 2010; Pignata et al. 2013; Smucker and Vis 2009; Triest et al. 2001). Other studies show that invertebrates have
been found to be more sensitive to habitat impacts (Feio et al. 2007; Hering et al. 2006; Pignata et al. 2013; Triest
20

-------
et al. 2001). Some studies have found that algae may be more variable than invertebrates (Mazor et al. 2006;
Mykra et al. 2012), or at least more variable at larger spatial scales (Springe et al. 2006). Finally, one analysis
looking at a combined assessment model found that an O/E type model in which the algae and invertebrates were
combined into one assessment indicator outperformed, in terms of sensitivity to disturbance, either assemblage
alone (Mendes et al. 2014).
Comparisons of algal assemblages with fish were less common. The few studies that were identified found
similar results to invertebrates, namely that there was weak concordance among assessments using algae and fish
(Carlisle et al. 2008), that diatoms generally were more sensitive to nutrients than fish, and that fish were more
sensitive to habitat impacts (Hering et al. 2006; Johnson et al. 2006; Justus et al. 2010; Smucker and Vis 2009).
They also found a better signal with fish to gradients along large spatial scales (whole basin) than diatoms,
presumably due to greater natural within basin variability of diatoms versus fish (Hering et al. 2006; Springe et al.
2006).
SPECIFIC POLLUTANT SOURCE APPLICATIONS
Algae have been used to study a variety of pollutant sources. They have been found to be sensitive to
pollutants derived from acid mine drainage (Schowe and Harding 2014; Smucker and Vis 2013; Zalack et al. 2010),
which likely includes both pH and metal sensitivities (Charles et al. 2006; Wunsam et al. 2002). They have also been
shown to be sensitive to urbanization, which may reflect the strong effect of conductivity per se on algae (Charles
et al. 2006; Ponader et al. 2008) as well as increased nutrient concentrations frequently associated with
urbanization (Paul and Meyer 2001; Walker and Pan 2006). Algal nitrogen isotopic signatures have also been used
to infer the source of nitrogen in urban settings, which may prove valuable in source tracking and, ultimately,
nutrient pollution management. In Japan, for example, the isotopic signature of algal nitrogen in an urban stream
was more closely related to sewage than fertilizer (Toda et al. 2002). Finally, algae have been shown to be sensitive
to agricultural land use, due to their sensitivities to nutrients as well as to sediment, since algal species differ in
their motility and abilities to move among fine substrates to access light (Black et al. 2011; Smucker and Vis 2011;
Vazquez et al. 2011). Nutrient sensitive taxa and overall diversity decreased, the relative abundance of nutrient
tolerant taxa increased, and motile species increased with increases in nutrients and sediment in these landscapes
(Smucker and Vis 2011; Vazquez et al. 2011).
ADDITIONAL RESEARCH HIGHLIGHTS
This section describes additional research highlights related to this algal indicator review that may be of value
to readers, including specific chemical applications, habitat applications, effects of variability, specific indicator
analyses, and methods. While this review did not focus on specific chemical effects, a number were reported in the
literature. For example, in addition to nutrients, algae are known to respond to a wide variety of conditions,
especially changes in pH, conductivity, and dissolved oxygen, and this sensitivity was reflected in several of the
21

-------
studies reviewed (Charles et al. 2006; Lebkuecher et al. 2011; Ponader et al. 2008; Wunsam et al. 2002). Another
study found that sewage discharges of soluble phosphorus resulted in large increases in the cyanobacteria,
especially the Oscillatoriales and Nostocales (Douterelo et al. 2004). In contrast, nitrogen fixing algal forms
(heterocystous cyanobacteria and diatoms with N-fixing symbionts) in California were observed to decline with
increasing nitrogen, but at relatively low concentrations (see above, Stancheva et al. 2013).
Findings related to habitat response are mixed. Most studies comparing assemblage responses (e.g.,
invertebrates versus algae) found that diatoms do not appear to respond to habitat gradients as well as
invertebrates or fish. However, other studies have found significant algal responses to habitat change. This is
especially true with increases in fine sediments, which tend to favor more motile diatom taxa (Pan et al. 2006). The
latter study did not compare the algal response to other assemblages, so it may be that algae do indeed respond,
just not as strongly as other taxa.
Algae vary spatially (Charles et al. 2006; Passy 2007; Weilhoefer and Pan 2006b), given differences in limiting
factors like light, flow, and substrate, even within one reach, not to mention across basins. This variability makes
classification for metric and index development, either explicitly (in MMI indices) or implicitly (in O/E type models),
critical (Charles et al. 2006), and this is a big component of many index development studies (Charles et al. 2006;
Fisher et al. 2010; Gevrey et al. 2004). Algae also vary over time (Taylor et al. 2007), and this variability can
increase with eutrophication (Korhonen et al. 2013). The time scale to which an assemblage responds to nutrients
may be several weeks long. Nutrient concentrations in streams in South Africa monitored one month to six weeks
prior to algal sampling were better predictors of algal response than grab samples taken during algal sampling
(Taylor et al. 2007). An area in need of more research is the degree to which biomass and assemblage structure
vary over time and whether the latter is less variable, emphasizing a potential further benefit of assemblage
response measures (Stevenson 2014).
As the proliferation of algal metrics and indices has increased across the globe, so too has analysis of metrics
and their performance. The first observation is that there is some general consensus that, for streams, biomass
measures are frequently highly variable in their response to nutrients (Porter et al. 2008; Stevenson et al. 2006).
However, one of the same studies also found that chlorophyll a and Cladophora biomass were related to nutrient
concentrations, so the highly variable responses are by no means universal and may depend on the biomass
measure chosen (Stevenson et al. 2006).
Biovolume responses are sometimes related to nutrient concentration, depending on the type of measure
used (e.g., relative versus total biovolume, Reavie et al. 2010), but more frequently not (Raunio and Soininen 2007;
Stancheva et al. 2011; Stancheva et al. 2012). In Canada, relative abundance was found to be better than
biovolume, which did respond to nutrients, but was more variable (Lavoie et al. 2006a; Lavoie et al. 2006b).
Diversity and autecological metrics also differ. Diversity metrics, described above, among the first algal metrics
used in assessment in the U.S. (Stevenson et al. 2010), vary in performance. Diversity metrics have sometimes
22

-------
exhibited poor correlation with nutrient concentration (Stancheva et al. 2011; Stancheva et al. 2012); this may be
due, somewhat, to the Gaussian response of diversity to nutrient concentrations, tending to increase at
intermediate concentrations (Wang et al. 2009). Other studies have found stronger responses of diversity metrics
(Gudmundsdottir et al. 2013). Overall, the majority of studies have found that autecological (tolerance, sensitivity)
and trait-based (growth form, motility) metrics generally exhibit better correlations and responses to nutrients
than diversity or richness metrics (Berthon et al. 2011; Blanco et al. 2012; Danielson et al. 2011; de la Rey et al.
2008a; Griffith et al. 2002; Stenger-Kovacs et al. 2013).
Lastly, there are a number of method improvements and novel methods that may help increase the
application of algae in water quality assessment. There have been improvements in microscopy that may reduce
variability in microscopic identification and increase the processing time for these types of analyses. These include
applications of confocal laser scanning microscopy in what is labeled "spectral fingerprinting" using spectral
emission signatures of algae (Larson and Passy 2005) to identify taxa, as well as new image analysis software (e.g.,
SHERPA) that has improved on previous efforts and can automate identification (Kloster et al. 2014). There also
continues to be development of novel molecular method applications, including application of DNA microarrays
using phylochip technology as well as next-gen sequencing of diatoms, which could, in theory, decrease processing
time and taxonomic variability, and increase sample sizes (Kermarrec et al. 2014; Metfies et al. 2007).
CONCLUSIONS
Algae are critical components of stream ecosystems, are relatively cheap and easy to measure, and sensitive
to nutrient pollution, making them a potentially useful indicator of ecosystem change. Their population and
biomass dynamics affect the food web of the entire stream ecosystem. Algal species composition and biomass are,
in turn, also affected by water quality and habitat alteration and can be informative indicators of environmental
condition. Because of these facts, algae affect aquatic life, recreational, and drinking water source uses, and are
excellent assessment endpoints that have been applied globally as ecological indicators. A wide variety of methods
and tools exist for sampling and assessing algae in streams that continue to proliferate and improve each year.
While algal indicators are promising tool for managers, the use of these indicators is not currently widespread. Less
than 50 percent of U.S. states appear to evaluate algae regularly. However, those using the algal assemblages have
applied them to both the development of nutrient and biocriteria, as well as assessment and stressor diagnosis.
Maine, Montana, and Kentucky appear to have the most comprehensive, current application of algal sampling,
incorporating both species composition and biomass measures into their assessment programs and into nutrient
criteria development. From the literature, it appears algal indicators are more widely employed routinely in
Europe, where they are used to assess water quality, biological condition, and identify water quality stressors like
nutrients and acidity. The European Union (EU) is ahead of the U.S. not only in applying this assemblage, but also in
working across jurisdictions to resolve methodological and interpretive differences in algal assessment
23

-------
information. EU methodologies and their application are well documented, which should help the U.S. in
developing consistent application.
ACKNOWLEDGMENT
Stefanie Gera (Tetra Tech, Inc.), Dacia Mosso (Tetra Tech, Inc.), and Kristen Parry (Tetra Tech, Inc.) helped
identify state documents and peer-reviewed manuscripts for consideration and their contributions were integral to
this project. Yukiko Ichishima (Tetra Tech, Inc.) helped with editing and reformatting.
The authors also wish to gratefully acknowledge the efforts and input of individuals who reviewed earlier
drafts, including: Sophie Greene, John William Carroll, Lesley D'Anglada, Ana-Maria Murphy-Teixidor, Mario
Sengco, Toby Stover, Izabela Wojtenko, Mark Barath, Bill Richardson Lauren Petter, Robie Anson, Forrest John,
Mike Bira, Gary Welker, Tina Laidlaw, Alfred Basil, Suesan Saucerman, Rochelle Labiosa, Hillary Snook, Diane
Switzer, Margherita Pryor, James Kurtenbach, Greg Pond, Louis Reynolds, David Melgaard, Chris Decker, Edward
Hammer, Robert Cook, Catherine Wooster-Brown, Terrence Fleming, Gretchen Hayslip, Robert Angelo, Kevin
Berry, Steve Kroeger, Kenneth Weaver and the Association of Clean Water Administrators - Monitoring, Standards
and Assessment Committee
24

-------
REFERENCES
Adams, K.E., Z.E. Taranu, R. Zurawell, B.F. Cumming, and I. Gregory-Eaves. 2014. Insights for lake management
gained when paleolimnological and water column monitoring studies are combined: A case study from
Baptiste Lake. Lake and Reservoir Management 30(1): 11-22.
Albertin, A. 2009. Nutrient Dynamics in Florida Springs and Relationships to Algal Blooms, University of Florida,
Gainesville, FL
Almeida, S.F.P., C. Elias, J. Ferreira, E. Tornes, C. Puccinelli, F. Delmas, G. Dorflinger, G. Urbanic, S. Marcheggiani, J.
Rosebery, L Mancini, and S. Sabater. 2014. Water quality assessment of rivers using diatom metrics
across Mediterranean Europe: A methods intercalibration exercise. Science of the Total Environment
476:768-776.
Alvarez-Bianco, I., S. Blanco, C. Cejudo-Figueiras, and E. Becares. 2013. The Duero Diatom Index (DDI) for river
water quality assessment in NW Spain: design and validation. Environmental Monitoring and Assessment
185(1):969-981.
Association of Clean Water Administrators. 2012. Use of Biological Assessment in State Water Programs: Focus on
Nutrients. Washington, DC.
Atazadeh, I., M. Sharifi, and M.G. Kelly. 2007. Evaluation of the trophic diatom index for assessing water quality in
River Gharasou, western Iran. Hydrobiologia 589:165-173.
B-Beres, V., P. Torok, Z. Kokai, E.T. Krasznai, B. Tothmeresz, and I. Bacsi. 2014. Ecological diatom guilds are useful
but not sensitive enough as indicators of extremely changing water regimes. Hydrobiologia 738(1):191-
204.
Barbour, M.T., J. Gerritsen, B.D. Snyder, and J.B. Stribling. 1999. Rapid Bioassessment Protocols for Use in Styreams
and Wadeable Rivers: Periphyton, Benthic Macroinvertebrates, and Fish, Second Edition. EPA-841-B-99-
002 EPA-841-B-99-002. US Environmental Protection Agency, Office of Water. Washington, DC.
Becker, M. 2012. Quality Assurance Project Plan (QAPP): Aquatic Life Response to Cultural Eutrophication in CT
Freshwater Wadeable Rivers and Streams (2012 - 2015).
Bellinger, B.J., C. Cocquyt, and C.M. O'Reilly. 2006. Benthic diatoms as indicators of eutrophication in tropical
streams. Hydrobiologia 573:75-87.
Bernstein, B.B. 2014. San Diego River Watershed Monitoring and Assessment Program. MR-RB9-2014-0001.
Surface Water Ambient Monitoring Program, San Diego Region, SWAMP.
Berthon, V., A. Bouchez, and F. Rimet. 2011. Using diatom life-forms and ecological guilds to assess organic
pollution and trophic level in rivers: a case study of rivers in south-eastern France. Hydrobiologia
673(1):259-271.
Besse-Lototskaya, A., P.F.M. Verdonschot, M. Coste, and B. Van de Vijver. 2011. Evaluation of European diatom
trophic indices. Ecological Indicators ll(2):456-467.
Besse-Lototskaya, A., P.F.M. Verdonschot, and J.A. Sinkeldam. 2006. Uncertainty in diatom assessment: Sampling,
identification and counting variation. Hydrobiologia 566:247-260.
Beyene, A., T. Addis, D. Kifle, W. Legesse, H. Kloos, and L. Triest. 2009. Comparative study of diatoms and
macroinvertebrates as indicators of severe water pollution: Case study of the Kebena and Akaki rivers in
Addis Ababa, Ethiopia. Ecological Indicators 9(2):381-392.
Beyene, A., A. Awoke, and L. Triest. 2014. Validation of a quantitative method for estimating the indicator power
of, diatoms for ecoregional river water quality assessment. Ecological Indicators 37:58-66.
Biggs, B.J.F. 2000. New Zealand Periphyton Guideline: Detecting, Monitoring and Managing Enrichment of Streams.
New Zealand Ministry for the Environment. Christchurch, New Zealand.
Biggs, B.J.F. and C. Kilroy. 2000. Stream Periphyton Monitoring Manual. New Zealand Ministry for the Environment.
Christchurch, New Zealand.
Birk, S., W. Bonne, A. Borja, S. Brucet, A. Courrat, S. Poikane, A. Solimini, W.V. van de Bund, N. Zampoukas, and D.
Hering. 2012. Three hundred ways to assess Europe's surface waters: An almost complete overview of
biological methods to implement the Water Framework Directive. Ecological Indicators 18:31-41.
Black, R.W., P.W. Moran, and J.D. Frankforter. 2011. Response of algal metrics to nutrients and physical factors and
identification of nutrient thresholds in agricultural streams. Environmental Monitoring and Assessment
175(l-4):397-417.
25

-------
Blanco, S., C. Cejudo-Figueiras, L Tudesque, E. Becares, L. Hoffmann, and L. Ector. 2012. Are diatom diversity
indices reliable monitoring metrics? Hydrobiologia 695(l):199-206.
Blinn, D.W. and D.B. Herbst. 2003. Use of diatoms and soft algae as indicators of stream abiotic determinants in
the Lahontan Basin, USA. California Regional Water Quality Control Board, Lahontan Region.
Borics, G., G. Varbiro, I. Grigorszky, E. Krasznai, S. Szabo, and K. Tihamer Kiss. 2007. A new evaluation technique of
potamo-plankton for the assessment of the ecological status of rivers. Archivfur Hydrobiologie.
Supplementband. Large rivers 17(3-4):465-486.
Busse, L.B. 2009. A New Tool for Water Quality Assessment - Algae as Bioindicators. California Water Boards.
Cao, Y., C.P. Hawkins, J. Olson, and M.A. Kosterman. 2007. Modeling natural environmental gradients improves the
accuracy and precision of diatom-based indicators. Journal of the North American Benthological Society
26(3) :566-585.
Carlisle, D.M., C.P. Hawkins, M.R. Meador, M. Potapova, and J. Falcone. 2008. Biological assessments of
Appalachian streams based on predictive models for fish, macroinvertebrate, and diatom assemblages.
Journal of the North American Benthological Society 27(l):16-37.
Chambers, P.A., C. Vis, R.B. Brua, M. Guy, J.M. Culp, and G.A. Benoy. 2008. Eutrophication of agricultural streams:
defining nutrient concentrations to protect ecological condition. Water Science and Technology
58(11):2203-2210.
Charles, D.F., F.W. Acker, D.D. Hart, C.W. Reimer, and P.B. Cotter. 2006. Large-scale regional variation in diatom-
water chemistry relationships: Rivers of the eastern United States. Hydrobiologia 561:27-57.
Danielson, T.J., C.S. Loftin, L. Tsomides, J.L. DiFranco, and B. Connors. 2011. Algal bioassessment metrics for
wadeable streams and rivers of Maine, USA. Journal of the North American Benthological Society
30(4):1033-1048.
Danielson, T.J., C.S. Loftin, L. Tsomides, J.L. DiFranco, B. Connors, D.L. Courtemanch, F. Drummond, and S.P. Davies.
2012. An algal model for predicting attainment of tiered biological criteria of Maine's streams and rivers.
Freshwater Science 31(2):318-340.
de la Rey, P.A., H. Roux, L. van Rensburg, and A. Vosloo. 2008a. On the use of diatom-based biological monitoring -
Part 2: A comparison of the response of SASS 5 and diatom indices to water quality and habitat variation.
Water Sa 34(l):61-69.
de la Rey, P.A., J. Taylor, A. Laas, L. Van Rensburg, and A. Vosloo. 2004. Determining the possible application value
of diatoms as indicators of general water quality: A comparison with SASS 5. Water Sa 30(3):325-332.
de la Rey, P.A., L. van Rensburg, and A. Vosloo. 2008b. On the use of diatom-based biological monitoring - Part 1: A
comparison of the response of diversity and aut-ecological diatom indices to water quality variables in the
Marico-Molopo River catchment. Water Sa 34(l):53-60.
Dela-Cruz, J., T. Pritchard, G. Gordon, and P. Ajani. 2006. The use of periphytic diatoms as a means of assessing
impacts of point source inorganic nutrient pollution in south-eastern Australia. Freshwater Biology
51(5):951-972.
Delgado, C., I. Pardo, and L. Garcia. 2010. A multimetric diatom index to assess the ecological status of coastal
Galician rivers (NW Spain). Hydrobiologia 644(l):371-384.
Delgado, C., I. Pardo, and L. Garcia. 2012. Diatom communities as indicators of ecological status in Mediterranean
temporary streams (Balearic Islands, Spain). Ecological Indicators 15(1):131-139.
Dell'uomo, A. and M. Torrisi. 2011. The Eutrophication/Pollution Index-Diatom based (EPI-D) and three new
related indices for monitoring rivers: The case study of the river Potenza (the Marches, Italy). Plant
Biosystems 145(2):331-341.
Dodds, W.K. 2006. Eutrophication and trophic state in rivers and streams. Limnology and Oceanography 51(1):671-
680.
Dodds, W.K., J.R. Jones, and E.B. Welch. 1998. Suggested classification of stream trophic state: Distributions of
temperate stream types by chlorophyll, total nitrogen, and phosphorus. Water Research 32(5):1455-1462.
Dodds, W.K., V.H. Smith, and B. Zander. 1997. Developing nutrient targets to control benthic chlorophyll levels in
streams: A case study of the Clark Fork River. Water Research 31(7):1738-1750.
Dodds, W.K.E.B.W. 2000. Establishing nutrient criteria in streams. Journal of the North American Benthological
Society 19(1): 186-196.
Douterelo, I., E. Perona, and P. Mateo. 2004. Use of cyanobacteria to assess water quality in running waters.
Environmental Pollution 127(3):377-384.
26

-------
Eloranta, P. and J. Soininen. 2002. Ecological status of some Finnish rivers evaluated using benthic diatom
communities. Journal of Applied Phycology 14(l):l-7.
Feio, M.J., S.F.P. Almeida, S.C. Craveiro, and A.J. Calado. 2007. Diatoms and macroinvertebrates provide consistent
and complementary information on environmental quality. Fundamental and Applied Limnology
169(3) :247-258.
Feio, M.J., S.F.P. Almeida, S.C. Craveiro, and A.J. Calado. 2009. A comparison between biotic indices and predictive
models in stream water quality assessment based on benthic diatom communities. Ecological Indicators
9(3) :497-507.
Fetscher, A.E., LB. Busse, and P.R. Ode. 2010. Standard Operating Procedures for Collecting Stream Algae Samples
and Associated Physical Habitat and Chemical Data for Ambient Bioassessments in California (Abstract).
Technical Report 0602. California Water Control Board.
Fetscher, A.E., R. Stancheva, J.P. Kociolek, R.G. Sheath, E.D. Stein, R.D. Mazor, P.R. Ode, and LB. Busse. 2014a.
Development and comparison of stream indices of biotic integrity using diatoms vs. non-diatom algae vs.
a combination. Journal of Applied Phycology 26(l):433-450.
Fetscher, E., M. Sutula, A. Sengupta, and N. Detenbeck. 2014b. Linking Nutrients to Alterations in Aquatic Life in
California Wadeable Streams. EPA/600/R-14/043. U.S. Environmental Protection Agency, Washington,
DC.
Fisher, J., A. Deflandre-Vlandas, M. Coste, F. Delmas, and H.P. Jarvie. 2010. Assemblage grouping of European
benthic diatoms as indicators of trophic status of rivers. Fundamental and Applied Limnology 176(2):89-
100.
Florida Department of Environmental Protection (FLDEP). 2014. General Biological Community Sampling SOP.
Fore, L.S. 2010. Evaluation of Stream Periphyton as Indicators of Biological Condition for Florida Streams. Florida
Department of Environmental Protection. Tallahassee.
Fore, L.S. and C. Grafe. 2002a. Using Diatoms to Assess the Biological Condition of Large Rivers in Idaho.
Freshwater Biology 47:2015-2037.
Fore, L.S. and C. Grafe. 2002b. Using diatoms to assess the biological condition of large rivers in Idaho (USA).
Freshwater Biology 47(10):2015-2037.
Gallo, L., M. Battegazzore, A. Corapi, A. de Filippis, A. Mezzotero, and L. Lucadamo. 2013. Environmental analysis of
a regulated Mediterranean stream based on epilithic diatom communities the Crati River case (southern
Italy). Diatom Research 28(2):143-156.
Gevrey, M., F. Rimet, Y.S. Park, J.L. Giraudel, L. Ector, and S. Lek. 2004. Water quality assessment using diatom
assemblages and advanced modelling techniques. Freshwater Biology 49(2):208-220.
Gillett, N.D., Y.D. Pan, K.M. Manoylov, and R.J. Stevenson. 2011. The role of live diatoms in bioassessment: a large-
scale study of Western US streams. Hydrobiologia 665(l):79-92.
Gomez, N. and M. Licursi. 2001. The Pampean Diatom Index (IDP) for assessment of rivers and streams in
Argentina. Aquatic Ecology 35(2):173-181.
Griffith, M.B., B.H. Hill, A. Herlihy, and P.R. Kaufmann. 2002. Multivariate analysis of periphyton assemblages in
relation to environmental gradients in Colorado Rocky Mountain streams. Journal of Phycology 38(1):83-
95.
Gudmundsdottir, R., S. Palsson, E.R. Hannesdottir, J.S. Olafsson, G.M. Gislason, and B. Moss. 2013. Diatoms as
indicators: The influences of experimental nitrogen enrichment on diatom assemblages in sub-Arctic
streams. Ecological Indicators 32:74-81.
Hausmann, S., D. F. Charles, J. Gerritsen, T.J. Belton. 2016. A diatom-based biological condition gradient (BCG)
approach for assessing and developing nutrient criteria for streams. Science for the Total Environment
562:914-927.
Harding, W.R., C.G.M. Archibald, and J.C. Taylor. 2005. The relevance of diatoms for water quality assessment in
South Africa: A position paper. WaterSa 31(l):41-46.
Heiskary, S., R.W. Bouchard, and H. Markus. 2013. Minnesota Nutrient Criteria Development for Rivers. Minnesota
Pollution Control Agency.
Herbst, D.B. and D.W. Blinn. 2008. Preliminary Index of Biological Integrity (IBI)for Periphyton in the Eastern Sierra
Nevada, California - Draft Report.
27

-------
Hering, D., R.K. Johnson, S. Kramm, S. Schmutz, K. Szoszkiewicz, and P.F.M. Verdonschot. 2006. Assessment of
European streams with diatoms, macrophytes, macroinvertebrates and fish: a comparative metric-based
analysis of organism response to stress. Freshwater Biology 51(9):1757-1785.
Hill, B.H., A.T. Herlihy, P.R. Kaufmann, R.J. Stevenson, F.H. McCormick, and C.B. Johnson. 2000. Use of periphyton
assemblage data as an index of biotic integrity. Journal of the North American Benthological Society
19(1) :50-67.
Hill, B.H., R.J. Stevenson, Y.D. Pan, A.T. Herlihy, P.R. Kaufmann, and C.B. Johnson. 2001. Comparison of correlations
between environmental characteristics and stream diatom assemblages characterized at genus and
species levels. Journal of the North American Benthological Society 20(2):299-310.
Hill, W.R., S.E. Fanta, and B.J. Roberts. 2009. Quantifying phosphorus and light effects in stream algae. Limnology
and Oceanography 54(l):368-380.
Idaho Department of Environmental Quality (IDEQ). 2002. Idaho River Ecological Assessment Framework.
Johnson, R.K., D. Hering, M.T. Furse, and R.T. Clarke. 2006. Detection of ecological change using multiple organism
groups: metrics and uncertainty. Hydrobiologia 566:115-137.
Justus, B.G., J.C. Petersen, S.R. Femmer, J.V. Davis, and J.E. Wallace. 2010. A comparison of algal,
macroinvertebrate, and fish assemblage indices for assessing low-level nutrient enrichment in wadeable
Ozark streams. Ecological Indicators 10(3):627-638.
Juttner, I., S. Sharma, B.M. Dahal, S.J. Ormerod, P.J. Chimonides, and E.J. Cox. 2003. Diatoms as indicators of
stream quality in the Kathmandu Valley and Middle Hills of Nepal and India. Freshwater Biology
48(ll):2065-2084.
Kahlert, M., M. Kelly, R.L. Albert, S.F.P. Almeida, T. Besta, S. Blanco, M. Coste, L. Denys, L Ector, M. Frankova, D.
Hlubikova, P. Ivanov, B. Kennedy, P. Marvan, A. Mertens, J. Miettinen, J. Picinska-Faltynowicz, J. Rosebery,
E. Tornes, S. Vilbaste, and A. Vogel. 2012. Identification versus counting protocols as sources of
uncertainty in diatom-based ecological status assessments. Hydrobiologia 695(1):109-124.
Karthick, B., J.C. Taylor, M. Mahesh, and T. Ramachandra. 2010. Protocols for Collection, Preservation and
Enumeration of Diatoms from Aquatic Habitats fro Water Quality Monitoring in India. The IUP Journal of
Soil and Water Sciences 3(l):25-60.
Kelly, M., S. Juggins, R. Guthrie, S. Pritchard, J. Jamieson, B. Rippey, H. Hirst, and M. Yallop. 2008. Assessment of
ecological status in UK rivers using diatoms. Freshwater Biology 53(2):403-422.
Kelly, M., L. King, and B.N. Chathain. 2009. The conceptual basis of ecological status assessments using diatoms.
Biology and Environment-Proceedings of the Royal Irish Academy 109B(3):175-189.
Kentucky Department of Environmental Protection (KDEP). 2009a. Standard Operating Procedure: Collection
Methods for Benthic Algae in Wadeable Waters.
Kentucky Department of Environmental Protection (KDEP). 2009b. Standard Operating Procedure: Field
Assessments of Benthic Algae Condition in Wadeable Waters.
Kentucky Department of Environmental Protection (KDEP). 2010. Integrated Report to Congress on the Condition of
Water Resources in Kentucky: Integrated Report to Congress on the Condition of Water Resources in
Kentucky. Frankfort.
Kermarrec, L., A. Franc, F. Rimet, P. Chaumeil, J.M. Frigerio, J.F. Humbert, and A. Bouchez. 2014. A next-generation
sequencing approach to river biomonitoring using benthic diatoms. Freshwater Science 33(l):349-363.
King, R.S., B.W. Brooks, J.A. Back, J.M. Taylor, and B.A. Fulton. 2009. Linking Observational and Experimental
Approaches for the Development of Regional Nutrient Criteria for Wadeable Streams. US EPA Region 6.
Dallas, TX.
Kireta, A.R., E.D. Reavie, G.V. Sgro, T.R. Angradi, D.W. Bolgrien, B.H. Hill, and T.M. Jicha. 2012a. Planktonic and
periphytic diatoms as indicators of stress on great rivers of the United States: Testing water quality and
disturbance models. Ecological Indicators 13(1):222-231.
Kireta, A.R., E.D. Reavie, G.V. Sgro, T.R. Angradi, D.W. Bolgrien, T.M. Jicha, and B.H. Hill. 2012b. Assessing the
condition of the Missouri, Ohio, and Upper Mississippi rivers (USA) using diatom-based indicators.
Hydrobiologia 691(1): 171-188.
Kloster, M., G. Kauer, and B. Beszteri. 2014. SHERPA: an image segmentation and outline feature extraction tool for
diatoms and other objects. Bmc Bioinformatics 15.
Korhonen, J.J., P. Kongas, and J. Soininen. 2013. Temporal variation of diatom assemblages in oligotrophic and
eutrophic streams. European Journal of Phycology 48(2):141-151.
28

-------
Lai, G.G., B.M. Padedda, T. Virdis, N. Sechi, and A. Luglie. 2014. Benthic diatoms as indicators of biological quality
and physical disturbance in Mediterranean watercourses: a case study of the Rio Mannu di Porto Torres
basin, northwestern Sardinia, Italy. Diatom Research 29(l):ll-26.
Lane, C.R., J.E. Flotemersch, K.A. Blocksom, and S. Decelles. 2007. Effect of sampling method on diatom
composition for use in monitoring and assessing large river condition. River Research and Applications
23(10): 1126-1146.
Larson, C. and S.I. Passy. 2005. Spectral fingerprinting of algal communities: a novel approach to biofilm analysis
and biomonitoring. Journal of Phycology 41(2):439-446.
Lavoie, I., S. Campeau, M.A. Fallu, and P.J. Dillon. 2006a. Diatoms and biomonitoring: should cell size be accounted
for? Hydrobiologia 573:1-16.
Lavoie, I., S. Campeau, M. Grenier, and P.J. Dillon. 2006b. A diatom-based index for the biological assessment of
eastern Canadian rivers: an application of correspondence analysis (CA). Canadian Journal of Fisheries and
Aquatic Sciences 63(8):1793-1811.
Lavoie, I., S. Campeau, N. Zugic-Drakulic, J.G. Winter, and C. Fortin. 2014. Using diatoms to monitor stream
biological integrity in Eastern Canada: An overview of 10 years of index development and ongoing
challenges. Science of the Total Environment 475:187-200.
Lebkuecher, J.G., S.M. Rainey, C.B. Williams, and A.J. Hall. 2011. Impacts of Nonpoint-Source Pollution on the
Structure of Diatom Assemblages, Whole-Stream Oxygen Metabolism, and Growth of Selenastrum
capricornutum in the Red River Watershed of North-Central Tennessee. Castanea 76(3):279-292.
Liu, Y., C. Liu, F.F. Zhong, S.L Hu, and W.X. Weng. 2013. Ecology of Diatoms in Yangtze River Basin, Hubei:
Implications for Assessment of Water Quality. In Progress in Environmental Protection and Processing of
Resource, Pts 1-4, ed. X. Tang, W. Zhong, D. Zhuang, C. Li and Y. Liu, pp. 139-145. ://WOS:000320828200029.
Lobo, E.A., D. Bes, L. Tudesque, and L. Ector. 2004a. Water quality assessment of the Pardinho river, RS, Brazil,
using epilithic diatom assemblages and faecal coliforms as biological indicators. Vie Et Milieu-Life and
Environment 54(2-3):115-125.
Lobo, E.A., V.L.M. Callegaro, G. Hermany, N. Gomez, and L. Ector. 2004b. Review of the use of microalgae in South
America for monitoring rivers, with special reference to diatoms. Vie Et Milieu-Life and Environment 54(2-
3):105-114.
Maine Department of Environmental Protection (MDEP). 2009. Protocols for Calculating the Diatom Total
Phosphorus Index (DTPI) and Diatom Total Nitrogen Index (DTNI)for Wadeable Streams and Rivers.
Augusta.
Maine Department of Environmental Protection (MDEP). 2014. Algae Sampling in Rivers and Streams.
http://www.maine.gov/dep/water/riionitoring/biomotiitoring/sampling/algae/riversandstreariis.htm.
Accessed July 18, 2014.
Mazor, R.D., T.B. Reynoldson, D.M. Rosenberg, and V.H. Resh. 2006. Effects of biotic assemblage, classification, and
assessment method on bioassessment performance. Canadian Journal of Fisheries and Aquatic Sciences
63(2):394-411.
Mendes, T., S.F.P. Almeida, and M.J. Feio. 2012. Assessment of rivers using diatoms: effect of substrate and
evaluation method. Fundamental and Applied Limnology 179(4):267-279.
Mendes, T., A.R. Calapez, C.L. Elias, S.F.P. Almeida, and M.J. Feio. 2014. Comparing alternatives for combining
invertebrate and diatom assessment in stream quality classification. Marine and Freshwater Research
65(7) :612-623.
Metfies, K., M. Berzano, C. Mayer, P. Roosken, C. Gualerzi, L. Medlin, and G. Muyzer. 2007. An optimized protocol
for the identification of diatoms, flagellated algae and pathogenic protozoa with phylochips. Molecular
Ecology Notes 7(6):925-936.
Montana Department of Environmental Quality. 2011a. Assessment Methodology for Determining Wadeable
Stream Impairment Due to Excess Nitrogen and Phosphorus Levels.
Montana Department of Environmental Quality. 2011b. Periphyton Standard Operating Procedure.
Montana Department of Environmental Quality. 2011c. Sample Collection and Laboratory Analysis of Chlorophyll-a
Standard Operation Procedure.
Montana Department of Environmental Quality. 2011d. Water Quality Assessment Methods.
29

-------
Moulton, S.R., J.G. Kennen, R.M. Goldstein, and J.A. Hambrook. 2002. Revised Protocols for Sampling Algal,
Invertebrate, and Fish Communities as Part of the National Water-Quality Assessment Program. 02-150.
USGS. Reston.
Mykra, H., T. Saarinen, M. Tolkkinen, B. McFarland, H. Hamalainen, K. Martinmaki, and B. Klove. 2012. Spatial and
temporal variability of diatom and macroinvertebrate communities: How representative are ecological
classifications within a river system? Ecological Indicators 18:208-216.
Nelson, C.E., D.M. Bennett, and B.J. Cardinale. 2013. Consistency and sensitivity of stream periphyton community
structural and functional responses to nutrient enrichment. Ecological Applications 23(1):159-173.
New Jersey Department of Environmental Protection. 2007. Standard Operating Procedures: Ambient Biological
Monitoring Using Benthic Macroinvertebrates.
Pan, Y.D., B.H. Hill, P.H. Husby, R.K. Hall, and P.R. Kaufmann. 2006. Relationships between environmental variables
and benthic diatom assemblages in California Central Valley streams (USA). Hydrobiologia 561:119-130.
Passy, S.I. 2007. Community analysis in stream biomonitoring: What we measure and what we don't.
Environmental Monitoring and Assessment 127(l-3):409-417.
Passy, S.I. and R.W. Bode. 2004. Diatom model affinity (DMA), a new index for water quality assessment.
Hydrobiologia 524(1):241-251.
Paul, M.J. and J.L. Meyer. 2001. Streams in the urban landscape. Annual Review of Ecology and Systematics 32:333-
365.
Picinska-Faltynowicz, J. 2009. Diatom phytobenthos as a tool for assessing the ecological status of Polish rivers.
Oceanological and Hydrobiological Studies 38:155-161.
Pignata, C., S. Morin, A. Scharl, D. Traversi, T. Schiliro, R. Degan, P. Bartley, M. Tu, H. Liu, F. Peres, M. Coste, W. Liu,
and G. Gilli. 2013. Application of European biomonitoring techniques in China: Are they a useful tool?
Ecological Indicators 29:489-500.
Ponader, K.C. and D.F. Charles, 2003. Understanding the relationship between natural conditions and loadings on
eutrophication: algal indicators of eutrophication for New Jersey streams. New Jersey Department of
Environmental Protection. Trenton.
Ponader, K.C. and D.F. Charles. 2005. New Jersey Periphyton Bioassessment Protocol Manual: Trophic Diatom
Inference Models and Index Development for New Jersey Wadeable Streams. New Jersey Department of
Environmental Protection. Trenton.
Ponader, K.C., D.F. Charles, and T.J. Belton. 2007. Diatom-based TP and TN inference models and indices for
monitoring nutrient enrichment of New Jersey streams. Ecological Indicators 7(l):79-93.
Ponader, K.C., D.F. Charles, T.J. Belton, and D.M. Winter. 2008. Total phosphorus inference models and indices for
coastal plain streams based on benthic diatom assemblages from artificial substrates. Hydrobiologia
610:139-152.
Porter, S.D., D.K. Mueller, N.E. Spahr, M.D. Munn, and N.M. Dubrovsky. 2008. Efficacy of algal metrics for assessing
nutrient and organic enrichment in flowing waters. Freshwater Biology 53(5):1036-1054.
Potapova, M. and D.F. Charles. 2007. Diatom metrics for monitoring eutrophication in rivers of the United States.
Ecological Indicators 7(l):48-70.
Potapova, M.G. and D.F. Charles. 2002. Benthic diatoms in USA rivers: distributions along spatial and
environmental gradients. Journal of Biogeography 29(2):167-187.
Potapova, M.G., D.F. Charles, K.C. Ponader, and D.M. Winter. 2004. Quantifying species indicator values for trophic
diatom indices: a comparison of approaches. Hydrobiologia 517(l-3):25-41.
Pringle, C.M. and T. Hamazaki. 1997. Effects of fishes on algal response to storms in a tropical stream. Ecology
78:2432-2442.
Raunio, J. and J. Soininen. 2007. A practical and sensitive approach to large river periphyton monitoring:
comparative performance of methods and taxonomic levels. Boreal Environment Research 12(l):55-63.
Reavie, E.D., T.M. Jicha, T.R. Angradi, D.W. Bolgrien, and B.H. Hill. 2010. Algal assemblages for large river
monitoring: Comparison among biovolume, absolute and relative abundance metrics. Ecological
Indicators 10(2):167-177.
Resh, V.H. 2008. Which group is best? Attributes of different biological assemblages used in freshwater
biomonitoring programs. Environmental Monitoring and Assessment 138(1-3):131-138.
Responsive Management. 2012. 1/l/est Virginia Residents' Opinions on and Tolerance Levels of Algae in 1/l/est
Virginia Water. Responsive Management. Harrisonburg, VA.
30

-------
Rhode Island Department of Environmental Management. 2011a. Quality Assurance Project Plan for Data Analysis:
Freshwater Numeric Nutrient Criteria Development. Office of Water Resources.
Rhode Island Department of Environmental Management. 2011b. Standard Operating Procedure for Collection of
Benthic Algae from Natural and Artificial Substrates. Office of Water Resources. Providence.
Rhode Island Department of Environmental Management. 2011c. Standard Operating Procedure for Measurement
of Benthic Algae and Non-Vascular Plant Cover by Viewing Bucket and Modified Pebble Count. Office of
Water Resources. Providence.
Rinella, D.J. and D.L Bogan. 2007. Development of Macroinvertebrate and Diatom Biological Assessment Indices
for Cook Inlet Basin Streams - Final Report. Prepared for the Alaska Department of Environmental
Conservation.
Rinella, D.J. and D.L Bogan. 2010. Testing Alaska's macroinvertebrate- and diatom-based stream condition indices
in select urbanized streams. Prepared for the Alaska Department of Environmental Conservation.
Robertson, D.M., D.J. Graczyk, P.J. Garrison, L Wang, G. LaLiberte, and R. Bannerman. 2006. Nutrient
concentrations ant their relations to the biotic integrity of wadeable streams in Wisconsin. US Geological
Survey and US Department of the Interior Professional Paper 1722.
Robertson, D.M., B.M. Weigel, and D.J. Graczyk. 2008. Nutrient concentrations and their relations to the biotic
integrity of nonwadeable rivers in Wisconsin. US Geological Survey and US Department of the Interior
Professional Paper 1754.
Rollins, S.L., M. Los Huertos, P. Krone-Davis, and C. Ritz. ND. Algae Biomonitoring and Assessment for Streams and
Rivers of California's Central Coast.
Rott, E., E. Pipp, and P. Pfister. 2003. Diatom methods developed for river quality assessment in Austria and a
cross-check against numerical trophic indication methods used in Europe. Algological Studies 110(1):91-
115.
Rott, E. and S.C. Schneider. 2014. A comparison of ecological optima of soft-bodied benthic algae in Norwegian and
Austrian rivers and consequences for river monitoring in Europe. Science of the Total Environment
475:180-186.
Salomoni, S.E., O. Rocha, V.L. Callegaro, and E.A. Lobo. 2006. Epilithic diatoms as indicators of water quality in the
Gravatai river, Rio Grande do Sul, Brazil. Hydrobiologia 559:233-246.
Salomoni, S.E., O. Rocha, G. Hermany, and E.A. Lobo. 2011. Application of water quality biological indices using
diatoms as bioindicators in the Gravatai river, RS, Brazil. Brazilian Journal of Biology 71(4):949-959.
Schneider, S.C. and E.A. Lindstrom. 2011. The periphyton index of trophic status PIT: a new eutrophication metric
based on non-diatomaceous benthic algae in Nordic rivers. Hydrobiologia 665(1):143-155.
Schowe, K.A. and J.S. Harding. 2014. Development of two diatom-based indices: a biotic and a multimetric index
for assessing mine impacts in New Zealand streams. New Zealand Journal of Marine and Freshwater
Research 48(2):163-176.
Smucker, N.J., M. Becker, N.E. Detenbeck, and A.C. Morrison. 2013. Using algal metrics and biomass to evaluate
multiple ways of defining concentration-based nutrient criteria in streams and their ecological relevance.
Ecological Indicators 32:51-61.
Smucker, N.J. and M.L Vis. 2009. Use of diatoms to assess agricultural and coal mining impacts on streams and a
multiassemblage case study. Journal of the North American Benthological Society 28(3):659-675.
Smucker, N.J. and M.L. Vis. 2011. Diatom biomonitoring of streams: Reliability of reference sites and the response
of metrics to environmental variations across temporal scales. Ecological Indicators 11(6):1647-1657.
Smucker, N.J. and M.L. Vis. 2013. Can pollution severity affect diatom succession in streams and could it matter for
stream assessments? Journal of Freshwater Ecology 28(3):329-338.
Soininen, J. and P. Niemela. 2002. Inferring the phosphorus levels of rivers from benthic diatoms using weighted
averaging. Archiv Fur Hydrobiologie 154(1):1-18.
Solak, C.N. and E. Acs. 2011. Water Quality Monitoring in European and Turkish Rivers Using Diatoms. Turkish
Journal of Fisheries and Aquatic Sciences ll(2):329-337.
Springe, G., L. Sandin, A. Briede, and A. Skuja. 2006. Biological quality metrics: their variability and appropriate
scale for assessing streams. Hydrobiologia 566:153-172.
Stancheva, R., A.E. Fetscher, and R.G. Sheath. 2011. A MODIFIED METHOD FOR QUANTIFYING STREAM-
INHABITING, NON-DIATOM BENTHIC ALGAE. Journal ofPhycology 47:S61-S61.
31

-------
Stancheva, R., A.E. Fetscher, and R.G. Sheath. 2012. A novel quantification method for stream-inhabiting, non-
diatom benthic algae, and its application in bioassessment. Hydrobiologia 684(l):225-239.
Stancheva, R., R.G. Sheath, B.A. Read, K.D. McArthur, C. Schroepfer, J.P. Kociolek, and A.E. Fetscher. 2013.
Nitrogen-fixing cyanobacteria (free-living and diatom endosymbionts): their use in southern California
stream bioassessment. Hydrobiologia 720(1): 111-127.
Stenger-Kovacs, C., E. Lengyel, LO. Crossetti, V. Uveges, and J. Padisak. 2013. Diatom ecological guilds as indicators
of temporally changing stressors and disturbances in the small Torna-stream, Hungary. Ecological
Indicators 24:138-147.
Stenger-Kovacs, C., L Toth, F. Toth, E. Hajnal, and J. Padisak. 2014. Stream order-dependent diversity metrics of
epilithic diatom assemblages. Hydrobiologia 721(l):67-75.
Stevenson, J. 2014. Ecological assessments with algae: a review and synthesis. Journal of Phycology 50(3):437-461.
Stevenson, J.R., B.H. Hill, A.T. Herlihy, L.L. Yuan, and S.B. Norton. 2008a. Algae-P relationships, thresholds, and
frequency distributions guide nutrient criterion development. Journal of the North American
Benthological Society 27(3):783-799.
Stevenson, R., Y. Pan, and H. Van Dam. 2010. Assessing environmental conditions in rivers and streams with
diatoms. The Diatoms: Applications for the Environmental and Earth Sciences, 2nd ed. Cambridge
University Press, Cambridge:57-85.
Stevenson, R.J., Y. Pan, K.M. Manoylov, C.A. Parker, D.P. Larsen, and A.T. Herlihy. 2008b. Development of diatom
indicators of ecological conditions for streams of the western US. Journal of the North American
Benthological Society 27(4):1000-1016.
Stevenson, R.J., A. Pinowska, A. Albertin, and J. Sickman. 2007. Ecological Conditions of Algae and Nutrients in
Florida Springs: The Synthesis Report. Florida Department of Environmental Protection. Tallahassee, FL
Stevenson, R.J., S.T. Rier, C.M. Riseng, R.E. Schultz, and M.J. Wiley. 2006. Comparing effects of nutrients on algal
biomass in streams in two regions with different disturbance regimes and with applications for developing
nutrient criteria. Hydrobiologia 561:149-165.
Stevenson, R.J. and J.P. Smol. 2003. Use of algae in environmental assessments. Academic Press, San Diego, CA.
Stevenson, R.J. and B. Wang. 2001. Developing and Testing Algal Indicators of Nutrient Status in Florida Streams.
Florida Department of Environmental Protection. Tallahassee.
Suplee, M.W., V. Watson, M. Teply, and H. McKee, 2009. How Green is Too Green? Public
Opinion of What Constitutes Undesirable Algae Levels in Streams. Journal of the American Water
Resources Association (JAWRA) 45(1):123-140.
Szulc, B. and K. Szulc. 2013. The use of the Biological Diatom Index (BDI) for the assessment of water quality in the
Pilica River, Poland. Oceanological and Hydrobiological Studies 42(2):188-194.
Tan, X., F. Sheldon, S.E. Bunn, and Q.F. Zhang. 2013. Using diatom indices for water quality assessment in a
subtropical river, China. Environmental Science and Pollution Research 20(6):4164-4175.
Tang, T., Q.H. Cai, and J.K. Liu. 2006. Using epilithic diatom communities to assess ecological condition of Xiangxi
River system. Environmental Monitoring and Assessment 112(l-3):347-361.
Taylor, J., M.J. van Vuuren, and A. Pieterse. 2007. The application and testing of diatom-based indices in the Vaal
and Wilge Rivers, South Africa. Water Sa 33(1).
Toda, H., Y. Uemura, T. Okino, T. Kawanishi, and H. Kawashima. 2002. Use of nitrogen stable isotope ratio of
periphyton for monitoring nitrogen sources in a river system. Water Science and Technology 46(11-
12):431-435.
Torrisi, M. and A. DelTUomo. 2006. Biological monitoring of some Apennine rivers (central Italy) using the diatom-
based Eutrophication/Pollution Index (EPI-D) compared to other European diatom indices. Diatom
Research 21(1): 159-174.
Torrisi, M., A. Dell'Uomo, and L. Ector. 2008. Assessment of quality of the Apennine rivers (Italy) using the diatom
indices: the River Foglia. Cryptogamie Algologie 29(1):45-61.
Triest, L., P. Kaur, S. Heylen, and N. De Pauw. 2001. Comparative monitoring of diatoms, macroinvertebrates and
macrophytes in the Woluwe River (Brussels, Belgium). Aquatic Ecology 35(2):183-194.
Triest, L., H. Lung'ayia, G. Ndiritu, and A. Beyene. 2012. Epilithic diatoms as indicators in tropical African rivers
(Lake Victoria catchment). Hydrobiologia 695(l):343-360.
US Environmental Protection Agency (USEPA). 2000. Evaluation Guidelines for Ecological Indicators. EPA-620-R-99-
005. Office of Research and Development. Washington, DC.
32

-------
USEPA. 2000. Nutrient Criteria Technical Guidance Manual: Rivers and Streams. EPA-822-B-00-002. Office of
Water, Office of Science and Technology. Washington, DC.
USEPA. 2002. Summary of Biological Assessment Programs and Biocriteria Development for States, Tribes,
Territories, and Interstate Commissions: Streams and Wadeable Rivers. EPA-822-R-02-048. Office of
Water. Washington, DC.
USEPA. 2009. National Rivers and Streams Assessment: Field Operations Manual. Office of Water. Washington, DC.
USEPA. 2011. A Primer on Using Biological Assessments to Support Water Quality Management. EPA-810-R-11-01.
Office of Water, Office of Science and Technology. Washington, DC.
USEPA. 2013a. Biological Assessment Program Review.Assessing Level of Technical Rigor to Support Water Quality
Management. EPA-820-R-13-001. Office of Water, Office of Science and Technology. Washington, DC.
USEPA. 2013b. National Rivers and Streams Assessment 2008-2009: Technical Report. Office of Wetlands, Oceans,
and Watersheds; Office of Research and Development. Washington DC.
USEPA. 2014. U.S. EPA Expert Workshop: Nutrient Enrichment Indicators in Streams: Proceedings April 16-18, 2013.
EPA-822-R-14-004. Office of Water. Washington, DC.
Vazquez, G., J.A. Ake-Castillo, and M.E. Favila. 2011. Algal assemblages and their relationship with water quality in
tropical Mexican streams with different land uses. Hydrobiologia 667(1):173-189.
Vinten, A.J.A., R.R.E. Artz, N. Thomas, J.M. Potts, L. Avery, S.J. Langan, H. Watson, Y. Cook, C. Taylor, C. Abel, E.
Reid, and B.K. Singh. 2011. Comparison of microbial community assays for the assessment of stream
biofilm ecology. Journal of Microbiological Methods 85(3):190-198.
Walker, C.E. and Y.D. Pan. 2006. Using diatom assemblages to assess urban stream conditions. Hydrobiologia
561:179-189.
Wang, Q., C.Y. Zhi, P.B. Hamilton, and F.X. Kang. 2009. Diatom distributions and species optima for phosphorus and
current velocity in rivers from ZhuJiang Watershed within a Karst region of south-central China.
Fundamental and Applied Limnology 175(2):125-141.
Wang, Y.K., R.J. Stevenson, and L. Metzmeier. 2005. Development and evaluation of a diatom-based index of Biotic
Integrity for the Interior Plateau Ecoregion, USA. Journal of the North American Benthological Society
24(4):990-1008.
Weilhoefer, C.L and Y.D. Pan. 2006a. Diatom-based bioassessment in wetlands: How many samples do we need to
characterize the diatom assemblage in a wetland adequately? Wetlands 26(3):793-802.
Weilhoefer, C.L and Y.D. Pan. 2006b. Diatom assemblages and their associations with environmental variables in
Oregon Coast Range streams, USA. Hydrobiologia 561:207-219.
West Virginia Department of Environmental Protection. 2014a. 303(d) Listing Methodology for Algae Blooms.
West Virginia Department of Environmental Protection. 2014b. Periphyton Collection Protocols.
Winter, J.G. and H.C. Duthie. 2000. Epilithic diatoms as indicators of stream total N and total P concentration.
Journal of the North American Benthological Society 19(l):32-49.
Wu, N.C., Q.H. Cai, and N. Fohrer. 2012. Development and evaluation of a diatom-based index of biotic integrity
(D-IBI) for rivers impacted by run-of-river dams. Ecological Indicators 18:108-117.
Wunsam, S., A. Cattaneo, and N. Bourassa. 2002. Comparing diatom species, genera and size in biomonitoring: a
case study from streams in the Laurentians (Quebec, Canada). Freshwater Biology 47(2):325-340.
Zalack, J.T., N.J. Smucker, and M.L. Vis. 2010. Development of a diatom index of biotic integrity for acid mine
drainage impacted streams. Ecological Indicators 10(2):287-295.
33

-------
APPENDIX I - SUMMARY TABLE OF U.S. STATE ALGAL INDICATOR ENDPOINTS, METHODS,
INTERPRETIVE TOOLS, AND USE IN CRITERIA DEVELOPMENT AND/OR ASSESSMENT1.



Use in Criteria Development/

State Endpoints
Methods
Interpretive Tools
Assessment
Citations
Benthic biomass (AFDM and
Quantitative multihabitat
Biomass estimate


Chi a)




Percent benthic cover
Visual transect point intercept
Percent cover


Benthic algal assemblage
composition
Quantitative multihabitat; diatom
and non-diatom
Diatom/Non-Diatom MMI
Analyzing thresholds of algal MMI
and component metrics.
Potential to add in statewide
aquatic life use (ALU) assessment.
(Busse 2009; Fetscher et al.
2010; Fetscher et al. 2014a;
Fetscher et al. 2014b)

Qualitative soft-algae method



Benthic diatom assemblage
composition

MMI

(Bernstein 2014)
Benthic algal assemblage
composition

Multidimensional ordination
Diversity Index

(Blinn and Herbst 2003)
Benthic diatom assemblage

MMI and taxonomic


composition

completeness
(Observed/Expected) models

(Rollins et al. ND)
Benthic diatom assemblage

MMI


composition



(Herbst and Blinn 2008)
Benthic biomass (Chi a)
USGS NAWQA methods
Biomass estimate
Unknown

1This review was likely not fully comprehensive, but was based on available literature and documents.
34

-------
State
Endpoints
Methods
Interpretive Tools
Use in Criteria Development/
Assessment
Citations

Benthic diatom assemblage
composition

MMI in development
TITAN analysis with relative
abundance data
Change-point analysis of
metrics
Boosted regression trees of
NMS axes

(Association of Clean Water
Administrators 2012; Becker
2012; Smucker and Vis 2013)

Benthic biomass (Chi a)


Use water column biomass in
assessment; Explored stressor
response relationships with


Water column biomass (Chi a)



FL
Percent benthic cover
Visual rapid periphyton survey
(cover, thickness and length)

percent cover and biomass;
Use biomass, percent cover, and
species dominance in combined
(Florida Department of
Environmental Protection
(FL DEP) 2014; Fore 2010;
Stevenson and Wang 2001)

Benthic algal assemblage
composition
Quantitative multihabitat method;
Passive periphytometers
Diatom/Non-diatom MMI and
biological condition gradient
(exploratory);
criteria approach

Algal growth potential




ID
Benthic diatom assemblage
composition
Quantitative richest targeted
benthic habitat
MMI
Exploring diatom data use in
criteria development
(Fore and Grafe 2002b;
Idaho Department of
Environmental Quality
(IDEQ) 2002)

Benthic biomass (Chi a)
Quantitative richest targeted
benthic habitat (known area)



KY
Percent benthic cover
Visual transect point-intercept
(cover and thickness, identify
green and red algae, general
abundance)


(Association of Clean Water
Administrators 2012;
Kentucky Department of

Benthic diatom assemblage
composition
Quantitative richest targeted
benthic habitat (known area)
Qualitative targeted habitat
Qualitative multihabitat composite
MMI
Diatom MMI used in assessment;
Exploring stressor-response
modeling of diatom metrics for
use in criteria development
(KDEP) 2009a, 2009b, 2010)
35

-------



Use in Criteria Development/

State Endpoints
Methods
Interpretive Tools
Assessment
Citations
Benthic biomass (Chi a)
Quantitative richest targeted
habitat;
Passive periphytometers



Percent benthic cover
Visual transect point-intercept
with viewing bucket (cover, length,
and thickness)



Benthic algal assemblage
	 composition
ME
Quantitative richest targeted
habitat;
Total P and Total N optima
used to develop tolerance
Assess sites with diatom metrics;
Used diatom data in stressor-
(Danielson et al. 2011;
Danielson et al. 2012; Maine
Department of
Environmental Protection
(MDEP) 2009, 2014)
Passive periphytometers
values (TVs);
TVs used to develop some ME
specific diatom metrics along
with general metrics;
Diatom metrics used to assign
sites to ALU tiers;
Also developing N and P
inference models;
response models to develop
numeric criteria
Benthic biomass (Chi a)
USGS protocols



Water column biomass (Chi a)
MN
Standard quantitative water
column sample


(Heiskary et al. 2013)
Benthic algal assemblage
USGS Protocols
Exploratory analysis of stock
Exploratory use in NNC Technical

composition

metrics
Document

Benthic biomass (Chi a)
Quantitative richest targeted
benthic habitat (known area)



Percent benthic cover
Visual transect (cover, color,


(Suplee et al. 2009, Montana
Department of
MT
condition, length, and thickness)


Benthic algal assemblage
Qualitative multihabitat
Metrics including MT specific
Indices used in assessment;
Environmental Quality
composition
composite;
diversity, siltation, and
Metrics were used in stressor-
2011a, 2011b, 2011c, 2011d)

Qualitative multihabitat point
pollution indices
response analysis to support


transect composite

adopted NNC development.

36

-------
State
Endpoints
Methods
Interpretive Tools
Use in Criteria Development/
Assessment
Citations

Benthic biomass (AFDM and
Chi a)
Quantitative richest targeted
benthic habitat (known area)




Percent benthic cover
EPA Rapid Bioassessment Protocol
(RBP) view bucket


(New Jersey Department of
Environmental Protection
NJ
Benthic algal assemblage
composition
Quantitative richest targeted
benthic habitat composite (known
area);
Qualitative targeted habitat
composite;
Passive periphytometers;
TP and TN Inference Models
using weighted averaging -
partial least squares;
Trophic Diatom Indices are
rescaled inference model
values (0-100)
Using models to develop
assessment tools and to support
NNC development
2003; Ponaderand Charles
2005; Ponader et al. 2007;
Ponader et al. 2008)

Benthic biomass (Chi a)
Quantitative richest targeted
benthic habitat (known area)
Periphytometers



Rl
Percent benthic cover
Visual transect point-intercept
with viewing bucket (cover, length,
and thickness)


(Rhode Island Department
of Environmental
Management 2011a, 2011b,
2011c)

Benthic algal assemblage
composition
Quantitative richest targeted
benthic habitat (known area);
Passive periphytometers
TITAN and Classification and
Regression Tree (CART)
analysis of metric response to
nutrients
Unknown


Benthic biomass (Chi a)
Quantitative richest targeted
benthic habitat (known area)



WV
Percent benthic cover
Visual transect segments (cover,
thickness)

Percent cover of >40% is used as
numeric translator of narrative
recreational use standard for a
single transect
(West Virginia Department
of Environmental Protection
2014a, 2014b)

Benthic algal assemblage
composition
Quantitative richest targeted
benthic habitat (known area)
Standard algal metrics
Exploring use of diatoms in NNC
development for ALU

USGS
Benthic biomass (AFDM and
Chi a)
Quantitative richest targeted
benthic habitat (known area)


(Moulton et al. 2002; Porter
et al. 2008; Potapova and

Water column biomass (Chi a)
Quantitative water column sample


Charles 2007)
37

-------
State
Endpoints
Methods
Interpretive Tools
Use in Criteria Development/
Assessment
Citations

Benthic algal assemblage
composition
Quantitative richest targeted
benthic habitat (known area);
Qualitative depositional habitat
composite;
Qualitative multihabitat composite
Standard algal metrics


EPA NARS
Benthic biomass (AFDM and
Chi a)
Quantitative multiple transect
composite


(USEPA 2013b)

Benthic algal assemblage
composition
Quantitative multiple transect
composite
Diatom MMI


The following states reported assessing algae to USEPA in 2002. They were not included above based on understanding of current application, but this information is included for
completeness.
AZ
Benthic algal assemblage
composition
Quantitative richest targeted
benthic habitat (known area);
Passive periphytometers


(USEPA 2002)
IN
Benthic algal assemblage
composition
Pilot project with USGS


(USEPA 2002)

Benthic biomass (Chi a)
Quantitative richest targeted
benthic habitat (known area)



KS
Benthic diatom assemblage
composition
Quantitative richest targeted
benthic habitat (known area);
Passive periphytometers
Limited taxa identification

(USEPA 2002)

Benthic biomass (Chi a)
Quantitative richest targeted
benthic habitat (known area)



MA
Benthic algal assemblage
composition
Quantitative richest targeted
benthic habitat (known area);
Passive periphytometers


(USEPA 2002)
38

-------




Use in Criteria Development/

State
Endpoints
Methods
Interpretive Tools
Assessment
Citations

Benthic diatom assemblage
Quantitative richest targeted




composition
benthic habitat (known area);



NM

Qualitative multihabitat;
Passive periphytometers


(USEPA 2002)

Benthic algal assemblage
Quantitative richest targeted



NC
composition
benthic habitat (known area)


(USEPA 2002)
ND
Benthic diatom assemblage
composition
Quantitative richest targeted
benthic habitat (known area)


(USEPA 2002)
NY
Benthic diatom assemblage
composition
Quantitative richest targeted
benthic habitat (known area)


(USEPA 2002)
OR
Benthic algal assemblage
composition
Quantitative richest targeted
benthic habitat (known area)


(USEPA 2002)

Benthic biomass (Chi a)
Quantitative richest targeted
benthic habitat (known area)



SD
Benthic algal assemblage
Quantitative richest targeted


(USEPA 2002)
composition
benthic habitat (known area);
Qualitative multihabitat;
Passive periphytometers


WA
Benthic algal assemblage
composition
Quantitative richest targeted
benthic habitat (known area)


(USEPA 2002)

Benthic biomass (Chi a)
Quantitative richest targeted
benthic habitat (known area)



Wl




(USEPA 2002)
Benthic algal assemblage
composition
Quantitative richest targeted
benthic habitat (known area)



Note,
"Chi a" = chlorophyll a;
"NNC" = numeric nutrient criteria;
"MMI" = multimetric index
39

-------
APPENDIX II - SAMPLE OF NUTRIENT THRESHOLDS DERIVED FROM
VARIOUS ALGAL ENDPOINTS
A number of studies in the U.S. and beyond, have explored the presence of thresholds in nutrient
concentrations associated with algal conditions for ecological phenomenological reasons (e.g., what nutrient
concentrations represents a change in ecosystem state) or for more applied reasons (e.g., at what nutrient
concentrations do users find algal response conditions that are unsuitable for recreation). This appendix reviews a
sample of these numeric nutrient thresholds for a variety of different endpoints.
A few studies were found that examined nutrient thresholds associated with algal responses, but this search
was far from exhaustive. One group of studies focused on identifying trophic state boundaries for streams. Trophic
boundaries are a reflection of primary production among other factors, and therefore reflect algal growth.
Boundaries associated with trophic states suggested thresholds at 20 ng/LTP and 300-600 ng/LTN for oligo-
mesotrophic streams and 50-60 ng/LTP and 600-750 or even 1500 ng/LTN for meso-eutrophic streams (Table 7).
Other studies put eutrophic streams at 70 ng/L TP and 1500 ng/L TN.
Other studies examined nutrient conditions associated with specific algal biomass levels, many of which have
been tied to adverse effects. These found maximum benthic chlorophyll levels in streams below 50-60 mg/m2 with
TP less than 16 or 25 ng/L and TN less than 115-145 or 700 |jg/L (Table 7). Maximum chlorophyll was below 100
mg/m2, when TP was less than 35-38 or 46 ng/L and TN less than 252-275, 470 or 1800 ng/L. Maximum
chlorophyll was below 200 mg/m2 with TP less than 75 or 90 ng/L and TN less than 650 or 1500 ng/L. Mean
chlorophyll levels below 50 mg/m2 were associated with TP concentrations of 60 and 62-65 ng/L and TN of 450-
470 ng/L. Mean chlorophyll levels below 100 mg/m2 were associated with TP concentrations of 197-221 ng/L and
TN of 1423-1600 ng/L. Finally, mean chlorophyll levels below 200 mg/m2 were associated with TP concentrations
of 415-1020 ng/L and TN of 3000-7570 ng/L. Thresholds in suspended chlorophyll (sestonic chlorophyll a)
response were identified at TP concentrations of 21, 64, and 70 ng/L TP and 927, 945, and 1169 ng/L TN (Table 7).
Studies were identified that looked at growth responses. These identified nutrient limitation for diatoms
occurring from 10-30 |jg/LTP and for algal biomass at 30 ng/LTP and 1000 ng/LTN. Saturated growth and
biomass accrual were identified at 50 and 82 ng/LTP, respectively (Table 7).
In terms of taxonomic changes that have been observed, the nuisance taxa Lyngbya and Vaucheria were
found to increase above TP concentrations of 33 and 26 ng/LTP and 250 and 284 ng/LTN respectively, in spring
streams in Florida (Table 7). In Texas, nuisance algal growth occurred at 200 ng/LTP. In contrast, heterocystous
cyanobacteria forms (N-fixing) and diatoms with N-fixing symbionts declined above 40 ng/L NH4-N and 75 ng/L
N03-N in another study, indicating the sensitivity of these taxa or growth forms to even low concentrations on
nitrogen. Other studies found that sensitive algal taxa began to decline at 20 ng/L TP, continued to decline at 40
Hg/LTP coincident with other assemblage changes, and then sensitive taxa were lost from 40-65 ng/L, when
40

-------
tolerant taxa began to increase (Table 7). These thresholds were reflected in index and metric nutrient thresholds
which were variously identified at 10 to 30 ng/LTP for some sensitive metrics, 50 ng/LTP for a diatom index, and
280 ng/L TP for a tolerant metric response. Equivalent TN thresholds were identified at 590 and up to 1790 ng/L
TN (Table 7).
41

-------
Table 7. Summary of nutrient threshold analyses.
Study Location
Response
TN (Mg/L)
TP (Mg/L)
Citation
Multiple
Stream oligo-mesotrophic
boundary
285-375
23-29
(Dodds 2006)
Stream meso-eutrophic
boundary
659-714
48-71
Multiple
Chi a < 200 mg/m2
<3000
<400
(Dodds 2000)
Chi a of 50 mg/m2 (< 100
mg/m2 most of the time)
470
60
Multiple
Stream oligo-mesotrophic
boundary (60 mg/m2 Chi a max)
700
25
(Dodds etal. 1998)
Stream meso-eutrophic
boundary (200 mg/m2 max)
1500
75
California
Heterocystous cyanobacteria
and diatoms with N-fixing
symbionts decline
75 (NOs -N)
40 (NH4 -N)

(Stancheva et al. 2013)
Florida
Lyngbya wollei
110 ( NOs -N)

(Albertin 2009)
Florida
Lyngbya wollei
230 (NOs -N)
250
28 (PO4-P)
33
(Stevenson et al. 2007)
Florida
Vaucheria sp.
261 (NOs-N)
284
Q_
O CD
Q_ CM
CM
CM
Mid-Atlantic region
Diatom nutrient limitation

10-30
(Stevenson et al. 2008a)
Michigan and
Kentucky/Indiana
Algal biomass
1000
30
(Stevenson et al. 2006)
Montana
50 mg/m2 mean Chi a target
450-470
62-65
(Dodds etal. 1997)
50 mg/m2 max Chi a target
115-145
16-20
100 mg/m2 mean Chi a target
1423-1600
197-221
100 mg/m2 max Chi a target
252-275
35-38
42

-------
Study Location
Response
TN (Mg/L)
TP (jig/L)
Citation

200 mg/m2 mean Chi a target
3000-7570
415-1020


200 mg/m2 max Chi a target
650
90

New Jersey
Diatom index response
200 (NOs -N)
50
(Ponader and Charles 2003)
Oak Ridge National Lab,
Tennessee
Benthic Algal Growth Saturation

50
(Hill etal. 2009)

Sensitive algal taxa decline

20

Ohio
Sensitive taxa loss and
assemblage change

40
(Smucker et al. 2013)
Sensitive diatoms lost, tolerant
taxa increase

65

Saturation

82

Texas (Aggregate Nutrient
Ecoregion IX)
Decline in biological integrity
(loss of algal, macrophytes, and
macroinvertebrate species),
decline in DO below levels
suitable for native fauna during
low flows, and increasing
nuisance algal growth

20
(second degradation
tier at 200)
(King et al. 2009)
Washington State, Nebraska
Algal metric responses
590-1790
30-280
(Black et al. 2011)
Wisconsin Non-wadeable
Streams
Suspended Chi a increases
927
64
(Robertson et al. 2008)

Suspended Chi a increases
1169
70

Wisconsin Wadeable Streams



(Robertson et al. 2006)

Benthic Chi a
609-1106
90


Eutrophic boundary
1500
75

Ontario/Quebec Canada
Suspended Chi a
945
21
(Chambers et al. 2008)

Benthic Chi a < 100 mg/m2
1800
46

43

-------
Study Location
Response
TN (Mg/L)
TP (jig/L)
Citation
Norway
Diatom multimetric threshold
begins

10
(Schneider and Lindstrom 2011)
Large diatom multimetric
responses occur

10-30
Note,
"Chi a" = chlorophyll a
44

-------