technical BRIEF
BUILDING A SCIENTIFIC FOUNDATION FOR SOUND ENVIRONMENTAL DECISIONS
A EPA
EPA's Water Security Test Bed
INTRODUCTION
The U.S. Environmental Protection Agency (EPA) is the lead federal agency responsible for
working with water utilities to protect water distribution systems from contamination and to clean
up systems that become contaminated. Intentional and unintentional contamination of
distribution systems can result in large amounts of water and miles of infrastructure that must be
cleaned to return the system to service.
Advancing the science and engineering of decontaminating pipe systems and of safely
disposing of high-volumes of contaminated water are high priorities for the EPA. The Agency
homeland security researchers developed the first-of-its-scale water security test bed (WSTB).
The first phase of the test bed, constructed at the Department of Energy's (DOE) Idaho National
Laboratory (INL), replicates a section of a typical municipal drinking water piping system with
roughly 450 feet of pipe and two fire hydrants laid out in an "L" shape. The eight-inch cement
mortar lined ductile iron pipes, used for the construction of the WSTB section were excavated
after twenty years of use for water conveyance (Figure 1). These pipes allow for technology
testing in an environment that simulates a typical operating
water distribution system. Researchers built the WSTB
above ground for easy access during experiments, for leak
detection, and for spill containment to protect the
groundwater.
Figure 1. Cement mortar lined,
ductile iron pipes, and auto-flushing
hydrant.
The purpose of conducting research at the WSTB facility is
to evaluate infrastructure decontamination technologies
previously tested by the EPA's Homeland Security
Research Program (HSRP) at the bench- and pilot-scale.
Using this simulated full-scale distribution system allows
for injection of contaminants that cannot be tested in
operating municipal water facilities. HSRP researchers
can then evaluate decontamination methodologies to
determine those that are best suited for use by water
utilities. The WSTB facility also enables testing of portable
water treatment technologies for the effective management
of the contaminated water that is discharged from the
contaminated pipeline into a 28,000-gallon lagoon. Lastly,
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decontamination of premise plumbing and household appliances can be evaluated in an
adjacent building at the site (Figure 2).
Contaminated Water Lagoon
Premise Plumbing
Downstream Sensor Box
WSTB End
WSTB Start
Upstream Sensor Box and Injection
Figure 2. Water security test bed (WSTB) and capability within the Idaho National
Laboratory site.
BACKGROUND
Homeland Security Presidential Directive 9 (HSPD-9. 1/30/2004) and Presidential Policy
Directive 21 (PPD-21. 2/12/2013) tasked EPA with responsibilities for water infrastructure
protection. In accordance with these directives, the HSRP has been conducting research to help
utilities protect against contamination incidents and help utilities rapidly detect and respond to
such incidents.
The WSTB full-scale facility has broad applicability for research into water system
decontamination. The facility provides for the injection of biological, chemical (including crude
oil), and radiological contaminants. The researchers are evaluating technologies and
methodologies to determine their efficacy for treating the water, and for decontaminating the
ductile iron pipe walls, water infrastructure appurtenances, premise plumbing and household
appliances. Researchers at the WSTB facility also conduct testing of full-scale innovative
portable water treatment technologies to treat contaminated water that is discharged. Effective
management of contaminated water (from clearwells, the distribution system, or contaminated
water from indoor and outdoor remediation activities) is needed to improve emergency
response, shorten response time, and improve preparedness. These studies can also inform
acceptance of such waters by water resource and recovery facilities (e.g., wastewater treatment
facilities). Research at the facility can also support cyber security defense and mitigation
approaches for water infrastructure operational technology. Support for field-testing of water
quality detection sensors and real-time modeling software is also possible. The WSTB facility is
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expandable to support research by other organizations and training for emergency personnel
and first responders.
WSTB Capability
The WSTB facility is equipped with sensors to detect contamination and injection points for the
introduction of contaminant simulants and decontamination agents. Removable coupons
(excised samples) are installed within the piping (Figure 3) and can be analyzed to determine
the adherence of contaminants to the pipe walls and to evaluate the efficacy of decontamination
efforts on pipe material and biofilm. A lined lagoon (28,000 gal) is constructed to contain water
flushed from the test bed.
At 200 feet, a 1-inch sevice connection line is connected to an adjacent building and provides
water to multiple types of household plumbing and home appliances. This allows for testing the
persistence of contaminants on
these household appliances and
on different premise plumbing
pipe material. Self-help methods
to decontaminate these
appliances are also evaluated.
INFRASTRUCTURE DECONTAMINATION EXPERIMENTS
Response to Microbiological Contamination in the Pipes
Bacillus atrophaeus subsp. globigii (BG) is a surrogate for Bacillus anthracis, the causative
agent of anthrax. BG spores are considered a resilient and conservative surrogate for most
microbiological water infrastructure contaminants. BG spores were injected into the WSTB pipe
and the persistence of the spores on the pipe material was evaluated. Chlorine dioxide
decontamination was chosen from successful pilot-scale decontamination experiments at EPA's
Test and Evaluation Facility in Cincinnati, Ohio. The number of BG spores was reduced by
about 6-log in the water, which was consistent with the pilot-scale experiment. However, even
with a higher concentration of chlorine dioxide at the field-scale, WSTB pipe wall
decontamination was not as effective as expected from the pilot-scale experiments. The
chlorine dioxide (100 mg/L) decontamination in the WSTB for 24 hours resulted in residual
spores remaining adhered to the cement-mortar pipe surface. There was only a 2-log reduction
at the WSTB as compared to a 4-log reduction at the pilot-scale. This was likely due to high
chlorine dioxide demand from the pipe, higher temperature for the over-ground pipes, and
inefficient transport of the disinfectant into dead end spaces. BG spores were found in the pipe
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Figure 3. Coupon sampling at the water security test bed pipe

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months after the experiment ended, even following draining of the pipe during the winter and
subsequent filling the next spring. The viability of the spores was not assessed. Testing of pipe
cleaning technologies will be conducted in future experiments.
Response to Chemical Contamination in the Pipes
The WSTB pipe was contaminated with the subnatant fraction of Bakken crude oil that had been
dissolved and mixed with local river water for 12 hours. This was meant to simulate an oil spill
on a water body. Decontamination was performed by flushing with clean water first, as typically
done by a water utility, followed by the addition of a surfactant. Persistence of the oil on the pipe
material was evaluated. Data collected during the crude oil contamination experiment suggest
that flushing the pipe with clean water was an effective decontamination method for the Bakken
crude oil. With clean water flushing, benzene detected in the WSTB pipe from the oil
contamination quickly dropped below the EPA prescribed drinking water Maximum Contaminant
Levels (MCLs). No total petroleum hydrocarbons or toluene, ethylbenzene and xylene
components were detected in the water. A surfactant was injected because it was assumed that
oily components could persist in the water phase or on the infrastructure surfaces. Online
sensor data and visual observation of foaming in the water samples indicated that the surfactant
may have persisted in the dead-end portions of the WSTB pipe for weeks after the initial
injection. Successful flushing with water makes surfactant addition ultimately unnecessary for
Bakken crude oil contaminations. This lingering foaming should be taken into consideration if a
surfactant is used during decontamination of a chemical in a drinking water distribution system.
Treatment Effectiveness of Microbiological Contamination in Water
BG spore contaminated water was collected in the lagoon. Four mobile disinfection technologies
were tested for their ability to disinfect large volumes of biologically contaminated "dirty" water
from the WSTB. The four technologies evaluated included: (1) Hayward Saline C™ 6.0
chlorination system, (2) advanced oxidation process (AOP) ultraviolet (UV)-ozone system
(Figure 4 below), (3) Solstreme™ UV water treatment system, and (4) WaterStep chlorinator.
Treatment effectiveness, capital cost, ease and speed of deployment, and operation were
documented. Results from the water treatment experiments indicate that disinfection of large
volumes of water contaminated with BG spores is feasible. All treatment units achieved at least
4-log removal of spores from the lagoon water over the course of the experiments, with some
units achieving 7-log reduction. Treated water volumes ranged from 1,250 to 5,000 gallons
(4,732 to 18,927 L) with experiments ranging from 5.5 hours to 1 day. It is likely that larger
volumes of water may need to be disinfected in a real world scenario, which would need the
scale up of portable units, or the use of multiple units. Data generated from this study
demonstrate the challenge of disinfection of contaminated water in the field due to the
disinfectant demand present in real world wash water, the potential for low temperature, and
disinfectant dissipation due to sunlight.
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Speece Cone


for ozone


diffusion

Ll /
Ozone

generator
11 —
(controller)
UV lamp and
ozone
generator
behind the
manifold
UV generator
(controller)
Figure 4. Mobile ozone/UV system.
Perfluorinated Compound Contamination
The portable on-site treatment of water contaminated with fire-fighting foam that contains
perfluorinated compounds was tested at the WSTB lagoon. Approximately 10,000 gallons of
chlorinated ground water from the WSTB well was contaminated with 5 gallons of 3M Light
Water™ aqueous firefighting foam (AFFF) containing perfluorinated compounds. EPA evaluated
the performance of 2 treatment technologies (1) Rembind™, an engineered powdered carbon
treatment media and (2) Filtrasorb®, a more traditional granular activated carbon media.
Preliminary results indicate that both systems were effective at reducing the perfluorinated
compounds in excess of 99,99%. However, the commercially available Remind material was too
fine to allow flow through it, and it had to be mixed with sand to allow adequate flow, an
important operational finding. The traditional granular activated carbon did not require any
manipulation and was ready to use in the field when it arrived at the site.
PREMISE PLUMBING and APPLIANCES DECONTAMINATION
Contamination of premise plumbing and appliances with BG spores and Bakken crude oil,
followed by disinfection with pH adjusted bleach and/or flushing was evaluated at the building
adjacent to the WSTB pipeline. Approximately 200 feet of 1-inch copper service line connects
the WSTB 8-inch pipeline to the building. This service line feeds the building where there are
multiple 6-inch sections of PVC, copper, and REX (crosslinked polyethylene) home plumbing,
which are then connected to a water heater, refrigerator, washing machine, dishwasher, and
utility sink as shown in Figure 5. The choice of disinfectant is to investigate an approach that
could be easily used by the homeowners.
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BG Spores
The BG spores were injected for an hour into the 1-inch copper service line just before the three
different premise plumbing pipe sections and appliances. Interestingly, some spores were found
in the cold water influent from the WSTB 8" pipeline before the experimental injection. These
BG spores are likely left over from past experiments in the big pipe and further highlights the
resilience of the spores and how critical and difficult it is to completely decontaminate water
infrastructure. After the BG spore contamination, decontamination proceeded by first draining
the hot water heater and then refilling with a solution of 4 gallons of bleach and 4 gallons of
vinegar into 47 gallons of uncontaminated tap water. The bleach solution flowed through all of
the pipe sections and entered all of the appliances with a contact time of 1 hour. The cold water
utility sink tap was flushed for 20 min, the hot water heater was drained, refilled and flushed with
hot water for 20 min, and all of the appliances were run once. The same procedure was
followed the next day but without the chlorination.
After chlorination and flushing, BG spores
were not found at the taps, but some were
found in the hot water heater and appliances.
The next day, 5 to 50 BG spores/100ml
(measured as colony forming units
(CFUs)/100 ml) were still found in the
appliances and coming out of the taps,
especially the hot water tap, which is supplied
by the hot water heater. The chlorination and
flushing resulted in a consistent 6-8 log
removal of spores from the water in the
plumbing pipes and appliances. The
remaining spores were likely caught in places
that the chlorination and flushing could not
reach adequately. Or, as mentioned
previously, the large pipeline could continue
to contribute as a source of contamination.
The data show that flushing and chlorination
do a good job removing a vast amount of the
contamination, but it is possible that low
numbers of spores can linger in premise
plumbing systems for longer periods of time.
Figure 5. Premise plumbing and appliances
The 6-inch sections of copper, REX and PVC coupons were swabbed and sampled before
contamination and again after chlorination and flushing. About a 2.3-log reduction on the PVC
and copper were observed and a 4-log reduction on the PEX. Similarly, lingering spores were
found after decontamination in the appliances, which could be from residual spores adhered to
the plumbing pipes or from dead spaces in the plumbing system where flushing was ineffective
or chlorine could not reach.
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Bakken Crude Oil
As in the large WSTB pipeline experiment, the "subnatant" water below the oil was injected for
an hour and was flushed through all of the plumbing and appliances. Decontamination followed
in order with (1) flushing the cold water tap (and refrigerator water) for 20 minutes, (2) then
draining the water heater, refilling and flushing the hot water plumbing for 20 minutes, and 3)
running the appliances for one cycle. Sampling and flushing was repeated the next day.
As in the 8-inch pipe experiment, large spikes of benzene were detected following injection.
After the first flushing/draining and thereafter, the levels dropped to the pre-injection baseline
level. Flushing was effective in decreasing the benzene levels to below the drinking water MCL.
Total petroleum hydrocarbons (made up of gas range organics, oil range organics and diesel
range organics) were also analyzed. There are no drinking water MCLs for these, but some very
low levels of some of the constituents were measured in the dishwasher and refrigerator water,
possibly due to either adherence to the plumbing material in those appliances and then leaching
or a remnant of the contaminant injection. No oily smell was detected following the second day
of flushing. Toluene, ethylbenzene, and xylene were also analyzed and any residual amounts
found were also well below the drinking water MCLs.
FUTURE EXPERIMENTS
Additional experiments are planned to:
•	Finish biological decontamination approaches that will include physical scouring of pipes
•	Evaluate decontamination of additional classes of chemical contaminants
•	Decontaminate pipes and appurtenances from radioactive contaminants
•	Continue evaluations and commercialization of innovative water treatment unit
processes
•	Evaluate cyber-attacks on system instrumentation, communications, and computer-
based systems for remote monitoring and control
In the event that additional resources/partners become available, the WSTB facility can be
expanded to provide a more complex hydraulic and operational network as shown below in
Figure 6. This expansion can be done with similar pipes from the original excavation and from
pipes from other partners or collaborators.
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Cirtulation
Pump
Effluent Tanks
Supply
Tank
Pressurizing
Figure 6. Future of the WSTB facility.
This would enable additional experiments involving:
•	Evaluations and applications of innovative real-time water quality detection
instrumentation and incident mitigation
•	Distribution system network modeling
•	Software calibration and verification
•	Additional cyber experiments in a more complex configuration
•	First responder, emergency personnel training, and homeowner self-help approaches
OUTREACH
EPA is opening up the test bed research capability to additional potential collaborators such as
agencies within the DOE, Department of Defense, the Department of Homeland Security,
universities, water utilities, and foundations interested in water security research. EPA is also
considering partners' needs as they build out the test bed to include service connections and
other types of pipe commonly found throughout water distribution systems.
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CONTACT INFORMATION
For more information, visit the EPA Web site at http://www2.epa.gov/homeland-security-
research
Technical Contacts: James Goodrich (Goodrich.iames@epa.gov)
Jeff Szabo (Szabo.ieff@epa.gov)
John Hall (hall.iohn@epa.gov)
General Feedback/Questions: Kathv Nickel (nickel.kathv@epa.gov)
If you have difficulty accessing this PDF document, please contact Kathv Nickel
(nickel.kathy@epa.gov or Amelia McCall (mccall.amelia@epa.gov) for assistance.
U.S. EPA's Homeland Security Research Program (HSRP) develops products based on
scientific research and technology evaluations. Our products and expertise are widely used in
preventing, preparing for, and recovering from public health and environmental emergencies
that arise from terrorist attacks or natural disasters. Our research and products address
biological, radiological, or chemical contaminants that could affect indoor areas, outdoor
areas, or water infrastructure. HSRP provides these products, technical assistance, and
expertise to support EPA's roles and responsibilities under the National Response
Framework, statutory requirements, and Homeland Security Presidential Directives.
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