EPA/600/R-16/264 I February 2017
www.epa.gov/homeland-security-research
United States
Environmental Protection
Agency
oEPA
Material Compatibility for Historic
Items Decontaminated with Gamma
Irradiation
Office of Research and Development
Homeland Security Research Program

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EPA 600/R-16/264
Material Compatibility for Historic Items
Decontaminated with Gamma Irradiation
National Homeland Security Research Center
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, NC 27711

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Disclaimer
The U.S. Environmental Protection Agency (EPA), through its Office of Research and Development's
(ORD) National Homeland Security Research Center (NHSRC), funded and managed this investigation
through contract EP-C-04-023 with ARCADIS U.S., Inc., Work Assignments (WA) 2-60, 5-60, 6-60 and
WA 3-58, and contract EP-C-15-008 with Jacobs Technology Inc., WA 0-076. This report has been peer
and administratively reviewed and has been approved for publication as an EPA document. It does not
necessarily reflect the views of the Agency. No official endorsement should be inferred. EPA does not
endorse the purchase or sale of any commercial products or services. Questions concerning this
document or its application should be addressed to:
Shannon Serre, PhD
Decontamination and Consequence Management Division
National Homeland Security Research Center
U.S. Environmental Protection Agency (MD-E343-06)
Office of Research and Development
109. T.W. Alexander Drive
Research Triangle Park, NC 27711
Phone:919-541-3817
Fax: 919-541-0496
E-mail: serre.shannon@epa.gov
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Acknowledgments
The U.S. Environmental Protection Agency (EPA), through the Office of Research and Development's
(ORD) National Homeland Security Research Center (NHSRC), funded and managed this study through
the On-site Laboratory Support Contract (EP-C-04-023) with ARCADIS U.S., Inc. and the Research
Laboratory Support Contract (EP-C-15-008) with Jacobs Technology Inc.
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Contents
Disclaimer	 i
Acknowledgments	ii
Figures	v
Tables	v
Acronyms and Abbreviations	vi
Executive Summary	vii
1	Project Description and Objectives	8
1.1	Background	8
1.2	Project Objectives	8
2	Experimental Approach	2
2.1	General Approach	2
2.2	Test Materials	2
2.2.1	Material Types	2
2.2.2	Coupon Preparation	4
2.2.3	Coupon Labeling Scheme	4
2.2.4	Coupon Equilibration	5
2.2.5	Preparation of Coupons for Gamma Irradiation	6
2.3	Gamma Irradiation	6
2.3.1	Dose Selection and Verification	6
2.3.2	Sterilization Effectiveness Checks	7
2.4	Assessment of Gamma Irradiation Effects	8
2.4.1	Visual Inspection	8
2.4.2	Technical Inspection	9
3	Results and Discussion	11
3.1	Category 1 Material Visual and Technical Inspections	11
3.1.1	Historical Oil Paintings	11
3.1.2	Light Hue Monochromatic Pigment Oil Paintings	12
3.1.3	Dark Hue Monochromatic Pigment Oil Paintings	13
3.1.4	Historical Documents	14
3.1.5	Historical Books	16
3.1.6	Historical Photographs	17
3.2	Category 2 Material Visual and Technical Inspections	18
3.2.1	Historical Pastel Paintings	18
3.2.2	Historical Leather Items	19
3.2.3	Historical Wood Items	20
3.2.4	Historical Porcelain Items	21
3.2.5	Historical Fabric Items	22
3.2.6	Historical Metal Items	23
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3.3 Dosage and Sterilization Verification	24
3.3.1	Dosage Verification Data	24
3.3.2	Sterilization Verification Data	24
4	Quality Assurance	26
5	Conclusions	28
5.1	Category 1 Materials (Priority Materials)	28
5.2	Category 2 Materials (Secondary Materials)	28
5.3	Biological Indicator Results	29
6	References	30
Appendix A: Biological Indicator Certificates of Analysis	31
Appendix B: Visual Impact Data Sheets	33
Appendix C: Short-Term and Long-term Technical Assessment Data	81
Appendix D: Certificates for Irradiation	90
Appendix E: Sterilization Verification Data	97
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Figures
Figure 2-1. Environmental chamber (a) and coupons stored in isolation chamber (b)	5
Figure 2-2. Test box with coupons	6
Figure 2-3. Example of severe visual impacts	9
Figure 3-1. Technical impact data from historical oil paintings	12
Figure 3-2. Technical inspection data from light hue monochromatic pigment oil paintings	13
Figure 3-4. Technical inspection data from historical documents	15
Figure 3-5. Technical inspection data from historical books	16
Figure 3-6. Technical inspection data from historical photographs	17
Figure 3-7. Technical impact data from historical pastel paintings	18
Figure 3-8. Technical impact data from historical leather items	19
Figure 3-9. Technical impact data from historical wood items	20
Figure 3-11. Technical impact data from historical fabric items	23
Tables
Table 2-1. Test Materials	4
Table 2-2. Coupon Identification	5
Table 2-3. Colorimetric Data Used For Spectrophotometric Technical Assessments	10
Table 3-1. Visual Impact Data from Historical Oil Paintings	11
Table 3-2. Visual Impact Data from Light Hue Monochromatic Pigment Oil Paintings	12
Table 3-3. Visual Impact Data from Dark Hue Monochromatic Pigment Oil Paintings	14
Table 3-4. Visual Impact Data from Historical Documents	15
Table 3-5. Visual Impact Data from Historical Books	16
Table 3-6. Visual Impact Data from Historical Photographs	17
Table 3-7. Visual Impact Data from Historical Pastel Paintings	18
Table 3-8. Visual Impact Data from Historical Leather Items	19
Table 3-9. Visual Impact Data from Historical Wood Items	20
Table 3-10. Visual Impact Data from Historical Porcelain Items	21
Table 3-11. Visual Impact Data from Historical Fabric Items	22
Table 3-12. Visual Impact Data from Historical Metal Items	23
Table 4-1. Data Quality Indicators for Critical Measurements	26
Table 4-2. Test Equipment Calibration Schedule	27
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Acronyms and Abbreviations
B.	Bacillus
Bl	biological indicator
CIE	International Commission on Illumination
AE*	color difference
D10	radiation dose required to reduce a viable population of a specific microorganism by 1 log
D65	standard illuminant defined by the International Commission on Illumination as representing
average daylight
DQI	data quality indicator
EPA	U.S. Environmental Protection Agency
HEPA	high-efficiency particulate air
ID	identification
kGy	kilogray
NHSRC	National Homeland Security Research Center
NIST	National Institute of Standards and Technology
ORD	Office of Research and Development
RH	relative humidity
SAL	sterility assurance level
WA	work assignment
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Executive Summary
This study was initiated by EPA's National Homeland Security Research Center (NHSRC) as part of a
comprehensive research program to provide scientific expertise and evaluation of actual and potential
decontamination technologies that can be used to restore and recover buildings and sensitive equipment
subjected to a biological weapon attack. The project described in this report was conducted at EPA's
Decontamination Technology Research Laboratory in Research Triangle Park, North Carolina, and was
designed to provide direct information on the impact of gamma irradiation on sensitive high-value
historical materials that can be viewed as surrogates for irreplaceable cultural objects that are commonly
found in museums, galleries, and archives.
The study addressed the impact of gamma irradiation on several types of materials. Test materials
included Category 1 (priority) materials that can be found in large quantities inside typical museum settings
and Category 2 (secondary) materials that were surrogates for high-value historical objects that are less
common in museum-type buildings. Priority materials tested in this study included historical oil paintings
and painting surrogates; archival documents, books, and photographs; and other museum-quality items
that are not easily removed from the site for off-site decontamination. Secondary materials were historical
pastel paintings, wood/furniture, porcelain/bisque, fabrics, metal and alloy objects, and leather.
Decontamination of historical materials using gamma irradiation at 30 and 50 kilograys (kGy) was
investigated to determine the short- and long-term effects on the test materials. Sample and reference
coupons were prepared for each historical material type. Pre-gamma irradiation visual and technical
assessments were performed on the sample and reference coupons to establish background values for
the materials tested. Biological indicators were included in the samples as a check for the effectiveness of
the gamma irradiation process. After irradiation, short-term visual and technical assessments were
performed on the sample coupons to determine any effects from the irradiation process. After 5 months,
visual and technical assessments of the coupons were performed to assess any long-term effects.
All Category 1 materials showed effects from gamma irradiation at both 30 and 50 kGy. All materials had
some visual changes at the short-term 30 kGy gamma irradiation level except the oil painting test strip
light hue and the archival photographs, both of which showed no impact. The long-term test samples
showed continued effects except for the oil painting test strip dark hue and the archival books, which
demonstrated no additional changes had occurred. Visual impacts were observed in the short-term
50 kGy samples except for the oil painting test strip dark hue and archival photographs, which showed no
visual impact. All long-term 50 kGy samples showed visual impacts. All samples at both irradiation levels
showed impacts in the technical assessment short-term data. Impacts for all samples increased for the
long-term assessments for both the 30 and 50 kGy samples.
All Category 2 materials showed effects from gamma irradiation at both the 30 and 50 kGy. The 30 kGy
short-term assessment samples showed visual changes except for the wood and metal samples, which
showed no impacts. Visual impacts were observed on the long-term 30 kGy pastel painting, leather, and
porcelain samples. The 30 kGy long-term wood, fabric, and metal samples had no visual changes. All
50 kGy short-term assessment samples showed visual impacts except for the fabric and metal samples.
Only the wood, fabric and metal samples showed no long-term visual impact at the 50 kGy irradiation level.
All samples at 30 and 50 kGy irradiation showed impacts in the technical assessment short-term data. The
impacts increased for all long-term assessment samples at both the 30 and 50 kGy irradiation levels.

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Bis were placed with each of the materials that were irradiated. Bis for the 30 kGy gamma irradiation
samples showed no growth for all samples with the exception of the B. atrophaeus Bl in the historical
documents sample, which showed growth. Three Bis for B. atrophaeus for the 50 kGy dose showed
growth. The historical wood, light hue monochromatic pigment oil painting, and historical pastel painting
sample Bis all showed growth. The remaining samples showed no growth. One Bl for the leather samples
showed growth for B. pumilus. The remaining sample Bis showed no growth. In the event that irradiation
was being considered for inactivation of B. anthracis on valuable objects, environmental samples should
be collected pre and post exposure to verify the item has been decontaminated.
1 Project Description and Objectives
Under Homeland Security Presidential Directive 10, EPA is tasked with coordinating appropriate federal
departments and agencies to develop comprehensive plans that "provide for seamless, coordinated
Federal, state, local, and international responses to a biological attack." As part of these plans, EPA in a
coordinated effort with the Department of Homeland Security, is responsible for "developing strategies,
guidelines, and plans for decontamination of persons, equipment, and facilities" to mitigate the risks of
contamination following a biological agent release.
EPA's NHSRC provides expertise and products that can be widely used to prevent, prepare for, and
recover from public health and environmental emergencies arising from terrorist threats and incidents.
The goal of the NHSRC decontamination research program is to provide expertise and guidance on the
selection and implementation of decontamination methods and provide the scientific basis for a significant
reduction in the time and cost of decontamination events.
The objective of this project was to investigate the impact of decontamination using gamma irradiation on
selected historical materials. This report presents results of the material compatibility assessments
approximately 3 weeks (short-term assessments) and 5 months after gamma irradiation (long-term
assessments). These assessments include the impact on the aesthetic (visual) value of historical objects
and instrumental spectrophotometric technical analyses for color changes in the materials.
1.1	Background
This project continued research of the effects of decontamination methods for biological agents on
materials identified as representative of types of irreplaceable objects or works of art found in museums
and/or archive settings. In the previous research, surrogate materials were checked for compatibility with
four decontamination methods: chlorine dioxide, hydrogen peroxide vapor, methyl bromide, and ethylene
oxide gas. This project investigated the effects of gamma irradiation, which has also been shown to be an
effective decontamination method for biological agents, on the surrogate test materials [1-3],
1.2	Project Objectives
The goal of this project was to examine the effects of gamma irradiation on irreplaceable and/or high-
value objects. Currently no experimental data are available that can predict the effects of gamma
irradiation on these objects. Future guidance for the use of gamma irradiation as a decontamination
method will depend on this information, and determining if gamma irradiation is compatible with these
items will allow them to be safely and effectively decontaminated. This report presents data resulting from
the following tasks:
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•	Prepared sample and reference coupons for gamma irradiation.
•	Performed initial visual and technical assessments of sample and reference coupons to get
sample baseline.
•	Prepared, packaged, and shipped coupons for gamma irradiation.
•	Performed short- and long-term visual and technical assessments and biological indicator (Bl)
evaluations after gamma irradiation.
2	Experimental Approach
2.1	General Approach
Decontamination of historical materials using gamma irradiation at 30 and 50 kilograys (kGy) was
investigated to determine any short- or long-term effects on the test materials. Sample coupons and
reference coupons (used to establish the baseline condition and as a comparison during the material
compatibility assessment) were prepared for each historical material type. The reference coupons, which
were never exposed to irradiation, underwent the same inspection scheme as the exposed coupons. This
process allowed the visual or structural (as appropriate) impact of the irradiation process on the test
materials to be assessed. All coupons were placed into a climate-controlled chamber for equilibration.
The relative humidity (RH) and temperature were set to values recommended for museum-type settings,
i.e., 70 ± 4 °F and 45 ± 5% RH. The coupons remained in the chamber for a minimum of 7 days. After
equilibration, pre-gamma irradiation visual and technical assessments were performed to establish
background values for the materials. The sample coupons were packaged and sent off-site to a
subcontracting laboratory for gamma irradiation. Biological indicators were included with each set of
samples as a check of the effectiveness of the gamma irradiation process. After gamma irradiation, the
samples were returned to the laboratory and placed in an environmental chamber along with the
reference coupons for a minimum of 7 days for conditioning. After equilibration, short-term (approximately
3	weeks after gamma irradiation) visual and technical assessments of the sample coupons were
performed. After the short-term assessments were completed, the sample and reference coupons were
placed in the environmental chamber. After 5 months, the samples were removed from the chamber and
long-term visual and technical assessments of the sample coupons were performed.
2.2	Test Materials
2.2.1 Material Types
The materials that underwent gamma irradiation compatibility testing were representative of irreplaceable
objects and works of art found in protected buildings and museums/galleries. These materials were
selected to represent historical and irreplaceable items in terms of quality, surface characteristics, and
structural integrity.
The materials tested were divided into two categories: Category 1 materials (also referred to here as
priority materials) were high-value historical objects that are found in large quantities inside a typical
museum setting and that often have large surface areas making them less suitable for off-site
decontamination (e.g., oil paintings). Category 2 materials (or secondary materials) were high-value
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historical objects that are less common in museum-type buildings (e.g., leather or porcelain objects).
Table 2-1 details the materials used in this study.
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Table 2-1. Test Materials
Material
Sample
Code
Description
Category 1 Materials (Priority Materials)
Historical oil painting
OPS
Two paintings used for this sample. Painting 1: child painted between 1800
and 1899. Painting 2: floral composition painted in 1885.
Oil painting test strip light hue
OLT
Painted by trained professional in the color Alizarin crimson.
Oil painting test strip dark hue
ODT
Painted by trained professional in the color Van Dyke brown.
Archival documents
DS
Handwritten letters from 1860 to 1890 and handwritten letters from 1909 to
1957.
Archival books
BS
Historic books from the late 19th and early 20th centuries.
Archival photographs
PS
Vintage photographs from the 19th and 20th centuries.
Category 2 Materials (Secondary Materials)
Pastel painting
PPS
Still life painting of fruit painted in 1905.
Leather
LS
Leather from the book cover of "Precious Thoughts" by John Ruskin, dated
1865, published by John Wiley and Sons of New York.
Wood
WS
1920s wooden coffee table.
Porcelain
PBS
Ten porcelain bread plates from Limoges Union Ceramique (UC) in France,
produced in the 20th century from 1909 to 1938.
Fabric
FS
Two fabrics used for this sample. Fabric 1: silk table cloth, unknown age.
Fabric 2: silk rope, unknown age.
Metal
MS
Brass serving tray dated 1928.
2.2.2	Coupon Preparation
Test coupons (5 cm x 5 cm) were prepared from the acquired historical materials by sawing, shearing, or
cutting with a rotary cutter depending on material type. Each coupon had a small hole punched or drilled
in a corner for attachment of an identification (ID) tag on a cotton string. Uniformity of test materials was
maintained by obtaining a large enough quantity of material such that multiple test sample coupons could
be made with presumably uniform characteristics (e.g., test coupons were cut from the interior rather than
the edge of a large piece of material). This was not possible, however, for some test sets of vintage
photographs due to their small size. In this instance, photographs taken at a similar time were used to
complete the test set.
Each test set consisted of five test coupons and three reference coupons. When applicable, test sets
were constructed in a way that allowed for assessment of a variety of materials (e.g., three types of
books, including color prints; two types of historical paintings; three types of hand-written documents).
After preparation, each set of test coupons was placed into a plastic container. The container was then
placed into an environmental chamber for a minimum of 7 days for coupon conditioning, as described in
section 2.2.4.
2.2.3	Coupon Labeling Scheme
Each coupon was identified by a unique sample code that included its associated work assignment
(project) number, material type, decontamination method, and process parameter information. Table 2-2
provides the sample coding used in this study.
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Table 2-2. Coupon Identification
Coupon Identification: WA60 M SM DDkGy R or NS R
Descriptor
Example Code
Description
WA60
WA60
Project identification
M
(Material)
OLT/ODT
Oil painting test strip light hue/oil painting test strip dark hue
OPS
Historical oil painting
PPS
Historical pastel painting
DS
Archival document
BS
Historical book
PS
Historical photograph
FS
Historical fabric
WS
Historical wood/furniture
MS
Historical metal/metal alloy
PBS
Historical porcelain/bisque
LS
Historical leather
SM
(sterilization method)
Gl
Gamma irradiation
DDkGy
(Dose)
50 kGy
30 kGy
50 kGy dose
30 kGy dose
NS
(control coupon)
NS
For reference coupons, the SM-DDkGy descriptor will be replaced with
NS (non-sterilzed)
Replicate
R
A-E (test coupons and non-sterilized reference coupons)
2.2.4 Coupon Equilibration
Before conducting initial visual and technical assessments, all test and reference coupons were
conditioned for a minimum of 7 days in an environmental chamber (Figure 2-1). The chamber was made
of opaque PVC with a clear acrylic door, which was fastened with a bolted flange and covered with an
opaque material to prevent light-catalyzed reactions from taking place during conditioning. The
temperature and humidity in the chamber were kept at settings typical of a museum-type environment
(i.e., approximately 70 °F and 45% RH). A Vaisala (Vantaa, Finland) temperature/RH sensor was used to
monitor the environmental conditions within the chamber. RH was controlled by a feedback loop using
LabVIEW software (National Instruments Corporation, Austin, TX, USA).
Figure 2-1. Environmental chamber (a) and coupons stored in isolation chamber (b).
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2.2.5 Preparation of Coupons for Gamma Irradiation
Test-specific boxes were prepared for gamma irradiation after the test coupon initial visual and technical
assessments. Each test box (Office Depot® brand white mailing boxes, 4 in. H x 9 1/4 in. W x 12 1/8 in. D
part no. 306689) had five test coupons suspended on cotton strings with ID tags. Coupons were hung so
they did not touch the sample box or each other. Sample test boxes with coupons and tags are shown in
Figure 2-2. Two types of Bis were placed inside the test box for checking sterilization effectiveness during
the gamma irradiation process, as described in section 2.3.2. The boxes were then closed and sealed
with carton sealing tape with paper backing (ProTapes Pro 184HD rubber high-tensile Kraft flat-back
carton sealing tape with paper backing, 7 mils thick, 55 yd L x2 in. W, ProTapes part no. Pro-184HD-
2x55-DBR). Each test box was labeled with a permanent marker according to the coupon identification
scheme given in Table 2-2. The label consisted of a description of the coupon material and a unique
sample code that corresponded to the sampling matrix. All boxes were weighed individually and their
weights recorded in the laboratory notebook, as well as on the chain of custody form submitted along with
samples to the subcontracting laboratory. All 30 kGy gamma irradiation sample boxes were packaged
together in one large box and all 50 kGy sample boxes were packaged together in a second box for
shipping.
Figure 2-2. Test box with coupons.
2.3 Gamma Irradiation
The test material coupons were sent to Sterigenics International LLC (Oak Brook, IL, USA,
http://www.steriqenics.com') for gamma irradiation. This facility performed the irradiations and verified
delivery of the correct doses and sterilization effectiveness.
2.3.1 Dose Selection and Verification
Gamma radiation is a form of pure energy, similar in many ways to microwaves and X-rays, characterized
by deep penetration and low dose rates. High-energy photons are emitted from an isotope source
(usually Cobalt 60) producing ionization (electron disruptions) throughout a product. In living cells, these
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disruptions result in damage to the DNA and other cellular structures. These photon-induced changes at
the molecular level cause the death of the organism or render the organism incapable of reproduction.
While able to kill microorganisms effectively, gamma irradiation does not create residuals or have
sufficient energy to impart radioactivity [4,5],
Two gamma irradiation doses (50 and 30 kGy) were selected for this study. Selection of the 50 kGy dose
was based on the radiation dose that was used to kill 1 g of dry Bacillus (B.) anthracis spores in a threat
envelope, with a sterility assurance equivalent to 14 logs of kill [6], The second operational sterilization
dose of 30 kGy was selected based on a theoretical radiosensitivity of dry B. anthracis spores and sterility
assurance level (SAL). The radiosensitivity of microorganisms and spores is expressed in terms of its D10
value, which is the radiation dose required to reduce a viable population of a specific microorganism by
1 log. The D10 for dry spores from most Bacillus species range from approximately 2 to 3.3 kGy. A D10
value for B. anthracis spores of 3.3 kGy was used in this study [6], The SAL is the probability of not more
than one viable spore remaining in a set number of sterilized items. The SAL of 1 x 10-3 was selected for
this study, which means that the probability is no more than one viable spore in a set of 1,000 irradiated
items [6], The lower dose also assumes that during actual decontamination, historical objects would be
bagged and removed from a building contaminated with B. anthracis spores, with the level of
contamination, or biological burden, initially reduced by use of a high-efficiency particulate air (HEPA)
vacuum prior to bagging.
Radiochromic film dosimeters (Far West Technology, Goleta, CA, USA) were used to verify the dosage of
radiation delivered to the coupons, i.e., quantification of absorbed radiation. The dosimeters were placed
on the test boxes during irradiation. Sterility testing was then performed on the irradiated samples to
determine if viable microorganisms were present (see section 2.3.2). This dose verification step
determined whether or not a change in sterilization dose was needed. The inherent reliability of the
gamma radiation process is outlined in detail in the standards document ANSI/AAMI/ISO 11137-1994 [7],
Additional information on dose kill curves can be found in a consolidated kill curve put together by the US
Department of Defense [8],
2.3.2 Sterilization Effectiveness Checks
Biological indicators were used for sterility assurance purposes. Two types of Bl spore strips (Mesa Labs,
Inc., Lakewood, CO, USA) were used for this study:
•	Bacillus (B.) pumilus - recommended by the manufacturer for validation of irradiation sterilization
processes.
•	B. atrophaeus - selected because B. atrophaeus is used as a surrogate for B. anthracis in many
NHSRC decontamination research projects.
Both Bis have a minimum population (bioburden) of 1,000,000 (1 x 106) spores. After exposure to the
sterilant, the strips were incubated at 30-35 °C, using the population assay procedure provided by the
manufacturer (Appendix A). Sterilization efficacy was determined by growth/no-growth of the biological
agent on the Bl spore strips that underwent the gamma irradiation treatment (as compared to amount of
biological agent recovered from positive control Bl spore strips, i.e., not exposed to the gamma irradiation
procedure). If the D10 for B. anthracis spores is 3.3 kGy/log reduction and the bioburden is 1 x 106, then
sterilization would require 3.3 kGy/log x 6 = 19.8 kGy to reduce the spore count by 6 logs, i.e., from 1 x
106 to 1 x 10°. After applying a SAL of 1 x 10 3, i.e., not more than one item out of 1,000 irradiated items
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would have a single viable spore, then the radiation dose should be 3.3 kGy/log x 9 = ~30 kGy to reduce
the spore count by 6 logs on 1,000 items..
2.4 Assessment of Gamma Irradiation Effects
2.4.1 Visual Inspection
Gamma irradiation visual impacts were expressed as the sum of the numerical values describing the
extent of noticeable physical changes in the tested materials. Seven physical characteristics were
checked for each test material coupon:
•	Changes in color
•	Changes in contrast
•	Fading of the material
•	Cracking or chipping
•	Brittleness and thinning
•	Legibility
•	Odors
A numerical value was assigned to each of the seven categories. The values ranged from 0, which
indicated no observable change), through 0.5 - indicating a very low change, 1 - a low change, 2 -
moderate change, and 3 which described a high level of change. For six data sets when the analyst
indicted an ultra-low (but perceptible) level of change sub-0.5 values (0.1 or 0.2) were used. The values
for the seven categories were summed together to get a cumulative impact score for the sample. The five
sample cumulative impact scores were then averaged to determine the average cumulative impact score
for the sample set. In addition to the use of numerical descriptors indicating a level of change in
appearance of the test coupon material, the analysts was also using narrative descriptions to note more
distinctive or unique problems with material condition. The coupon-specific narrative descriptions of
changes were noted along with an associated numerical descriptor in the respective short- and long-term
visual assessment check lists (Appendix B). The combined use of numerical and narrative descriptors
most faithfully explains analyst observation of visual changes in the post-exposure condition of historical
materials and historical material surrogates. All investigated materials were photodocumented, which was
a suitable tool for gross visual changes (an example of severe photodocumentation visual impacts is
shown in Figure 2-3). However, photography did not capture subtle changes (e.g., glossiness) in material
appearance very well. These changes were documented using narrative descriptors.
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Figure 2-3. Example of severe visual impacts.
2.4.2 Technical Inspection
Technical assessments were performed on the test materials by spectrophotometric analysis. A
spectrophotometer (CM-2500c, Konica Minolta Sensing, Japan) was used to measure the color change of
the coupons. Instrument parameters were set as follows:
•	llluminant 1: D65
•	llluminant 2: A
•	Observer angle: 2°
•	Color spacing: L*a*b*, E*
D65 simulates daylight (color temperature of 6504K including ultraviolet light), and A simulates an
incandescent lamp (color temperature of 2856K). The observer angle of 2° is from the International
Commission on Illumination (CIE). CIE originally defined the standard observer angle in 1931 using a 2°
field of view. L*a*b* color spacing includes all colors perceivable to the human eye. L* is the lightness of
the sample, a* is the red-green component, and b* is the yellow-blue component of the sample. E* is the
total color difference between the initial reading and the sample reading. For data analysis, the color
difference (AE*) tolerance was set at 1.0, assuming that a AE* of 1.0 is the smallest color difference that
the human eye can see (i.e., any AE* less than 1.0 is imperceptible and any AE* greater than 1.0 is
noticeable), it might be considered a "best case scenario" of the human eye perception of color
differences.
An initial reading was taken with the spectrophotometer of each sample coupon. The surface of the
coupon was read in a 4 cm x 4 cm grid pattern, moving 1 cm for each reading for a total of 16 readings.
The 16 readings were averaged to get a composite reading for the coupon surface. After the coupons
were irradiated, the short-term samples were measured using the same spectrophotometer settings and
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sampling procedure used for the initial sample readings. The initial sample readings were used to
calculate any color changes in the coupon surfaces. This process was repeated after 5 months to
determine any long-term effects of gamma irradiation.
Table 2-3 lists colorimetric data used for technical assessments of post-exposure material changes due to irradiation.
Table 2-3. Colorimetric Data Used ForSpectrophotometric Technical Assessments [9]
Data
Description
Color difference dE* ab
(AE*ab, D65)
The difference between two colors can be calculated using an equation developed
by the International Commission on Illumination (CIE) in 1976 called AEab. This
equation calculates the linear (Euclidian) distance between two points in the L*a*b*
three-dimensional space. D65 is a code that identifies a light source; D 65 is a
standard llluminant; average daylight (including ultraviolet wavelength region) with a
correlated color temperature of 6504 K; used for measuring specimens that will be
illuminated by daylight including ultraviolet radiation.
L*(D65)
The absolute value of lightness in the L*a*b* color space
a* (D65)
The absolute value of a chromaticity coordinate in the L*a*b* color space
b* (D65)
The absolute value of b chromaticity coordinate in the L*a*b* color space
d L*(D65)
The change of lightness in the L*a*b* color space (brighter/darker)
d a* (D65)
The change of the chromaticity coordinate a* in the L*a*b* color space (+a* is the red
direction, -a* is the green direction)
d b* (D65)
The change of the chromaticity coordinate a* in the L*a*b* color space (+b* is the
yellow direction, and -b* is the blue direction)
Lightness evaluation (D65)
The parameter that describes the subjective brightness perception of a color for
humans along a lightness-darkness axis (color amplitude) - defines the difference
between bright colors and dark colors
Saturation evaluation (D65)
The saturation of a color is determined by a combination of light intensity and how
much it is distributed across the spectrum of different wavelengths
Hue evaluation (D65)
Hue is the degree to which a stimulus can be described as similar to or different from
stimuli that are described as red, green, blue, and yellow (the unique hues)
Metameric index (D65)
Parameter that describes a phenomenon in which two colors appear the same under
one light source but different under another. In this study, secondary illumination was a
standard llluminant A, i.e., incandescent light with a correlated color temperature of
2856 K; used for measuring specimens that will be illuminated by incandescent lamps
10

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3 Results and Discussion
Results of this investigation for both short- and long-term effects of gamma irradiation on the historical
materials are discussed in this section. Visual and technical assessment results at two gamma radiation
doses (30 and 50 kGy) are described for each historical material in Categories 1 and 2. Following the
short-term assessments (~ 3 weeks post-sterilization), all test samples were stored in an environmental
chamber, and long-term assessments were performed 5 months later. Visual impact data sheets for
category 1 and 2 materials are in Appendix B. Technical assessment data sheets for category 1 and 2
materials are in Appendix C. The procedures for visual and technical assessments are given in Section
2.4.1 and 2.4.2 respectively.
3.1 Category 1 Material Visual and Technical Inspections
3.1.1 Historical Oil Paintings
3.1.1.1 Visual Inspection
Visual impacts were assessed of 30 kGy and 50 kGy gamma irradiation of historical oil paintings. Visual
inspection of this material type at 30 kGy irradiation resulted in both short-term and long-term cumulative
impact scores of 0.6. The total cumulative impact score for the 30 kGy samples was 1.2, which shows a
low level of change in the tested material. For the 50 kGy samples, the short-term cumulative impact
score was 0.1 and the long-term cumulative impact score was 0.7. The total cumulative impact score for
the 50 kGy samples is 0.8. This shows low impact on the tested material. This data are summarized in
Table 3-1.
Table 3-1. Visual Impact Data from Historical Oil Paintings
Material
Visual Changes 30 kGy

Visual Changes 50 kGy

Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Historical oil
paintings
0.6
0.6
1.2
0.1
0.7
0.8
3.1.1.2 Technical Inspection
Technical assessments of historical oil paintings were also made after 30 kGy and 50 kGy gamma
irradiation. The 30 kGy short-term assessment samples showed a slight average color change after
irradiation of 0.980. The long-term assessment color change for the 30 kGy samples increased to 4.582.
The 50 kGy short-term samples showed a color change average of 1.804. For the long-term samples, the
color change average increased to 3.986. These data are illustrated in Figure 3-1.
11

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Historical Oil Paintings short term Data
¦ Long-term Data
30 kGy data
50 kGy data
Figure 3-1. Technical impact data from historical oil paintings. Red dashed line indicates the smallest color
difference that the human eye can perceive, dE*ab=1.
3.1.2 Light Hue Monochromatic Pigment Oil Paintings
3.1.2.1 Visual Inspection
Visual impacts were assessed from both 30 and 50 kGy gamma irradiation of these test samples. The
30 kGy samples had a short-term cumulative impact score of 0 and a long-term cumulative impact score
of 0.4. The total cumulative impact score for the 30 kGy samples was 0.4, which showed a low level of
impact on the tested material. The 50 kGy sample short-term cumulative impact score was 0.18, and the
long-term cumulative impact score was 0.6. The total cumulative impact score for the 50 kGy samples
was 0.78, showing a low impact on the tested material. These data are summarized in Table 3-2.
Table 3-2. Visual Impact Data from Light Hue Monochromatic Pigment Oil Paintings
Material
Visual Changes 30 kGy

Visual Changes 50 kGy

Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Light hue
monochromatic
pigment oil
painting
0
0.4
0.4
0.18
0.6
0.78
12

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3.1.2.2 Technical Inspection
Technical assessments were made of the light hue monochromatic pigment oil paintings after 30 and
50 kGy gamma irradiation. The 30 kGy short-term assessment samples had an average color change
after irradiation of 1.344. The long-term assessment had an average increase to 5.488. The 50 kGy short-
term assessment samples showed more of a color change than the 30 kGy samples, with an average of
3.246. The long-term assessment sample average color change increased to 4.206. These data are
illustrated in Figure 3-2.
in
to
Q_
.Q
to
*
a< 3
ao
to
OJ
>
to 2
OiljPainting Test Strip Light
Short-term Data
Long-term Data
30 kGy data
50 kGy data
Figure 3-2. Technical inspection data from light hue monochromatic pigment oil paintings. Red dashed line
indicates the smallest color difference that the human eye can perceive, dE*ab=1.
3.1.3 Dark Hue Monochromatic Pigment Oil Paintings
3.1.3.1 Visual Inspection
Visual impacts from 30 and 50 kGy gamma irradiation were assessed on this set of test samples. The
30 kGy samples had a short-term cumulative impact score of 1, and the long-term cumulative impact
score was 0. The total cumulative impact score for the 30 kGy samples was 1, which shows a low level of
impact on the tested material. The 50 kGy samples had a short-term cumulative impact score of 0 and a
long-term cumulative impact score of 0.2. The total cumulative impact score for the 50 kGy samples is
0.2, again showing a low impact on the tested material. These data are summarized in Table 3-3.
13

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Table 3-3. Visual Impact Data from Dark Hue Monochromatic Pigment Oil Paintings

Visual Changes 30 kGy

Visual Changes 50 kGy

Material
Short-Term
Long-Term
Total
Short-Term
Long-Term
Total

Cumulative
Cumulative
Cumulative
Cumulative
Cumulative
Cumulative

Impact Score
Impact Score
Impact
Impact Score
Impact Score
Impact
Dark hue
monochromatic
pigment oil
painting
1
0
1
0
0.2
0.2
3.1.3.2 Technical Inspection
Technical assessments were made of the impact of 30 and 50 kGy gamma irradiation on light hue
monochromatic pigment oil paintings. The 30 kGy short-term assessment samples showed an average
color change after irradiation of 1.590. After the long-term assessment, the average color change for the
30 kGy samples increased to 3.520. The 50 kGy short-term assessment samples showed a color change
average of 1.562. The long-term assessment average color change increased to 3.134. These data are
illustrated in Figure 3-3.
Oil Painting Test Strip Dark
Short-term Data
I Long-term Data
in
to
O
.Q 3
to
*
OJ
M 2
(O
OJ
>
30 kGy data
50 kGy data
Figure 3-3. Technical impact data from dark hue monochromatic pigment oil paintings. Red dashed line
indicates the smallest color difference that the human eye can perceive, dE*ab=1.
3.1.4 Historical Documents
3.1.4.1 Visual Inspection
14

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Visual impacts on historical document test samples were assessed after 30 and 50 kGy gamma
irradiation. The 30 kGy samples had a short-term cumulative impact score of 0.82 and a long-term
cumulative impact score of 0.5. The total impact score for the 30 kGy samples was 1.32, showing a low to
moderate impact on the documents. The 50 kGy sample short-term cumulative impact score was 0.18
and the long-term cumulative impact score was 0.5. The total cumulative impact score for the 50 kGy
samples of 0.68 shows a low impact on the tested material. These data are summarized in Table 3-4.
Table 3-4. Visual Impact Data from Historical Documents
Material
Visual Changes 30 kGy

Visual Changes 50 kGy

Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Historical
documents
0.82
0.5
1.32
0.18
0.5
0.68
3.1.4.2 Technical Inspection
Technical assessments were made of the historical documents after 30 kGy and 50 kGy gamma
irradiation. The 30 kGy samples showed an average short-term color change after irradiation of 1.324.
The long-term assessment showed the average color change for the 30 kGy samples increased to 1.448.
The 50 kGy samples showed less of a color change than the 30 kGy samples in the short-term
assessment with an average of 1.022. After the long-term assessment, the average color change
increased to 1.746. These data are illustrated in Figure 3-4.
Short-term Data
¦ Long-term Data
Historical Documents
30 kGy data	50 kGy data
Figure 3-4. Technical inspection data from historical documents. Red dashed line indicates the smallest color
difference that the human eye can perceive, dE*ab=1.
15

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3.1.5 Historical Books
3.1.5.1 Visual Inspection
Visual impacts on historical books were assessed from gamma irradiation at both 30 and 50 kGy. The
30 kGy samples showed a short-term cumulative impact score of 0.52 and a long-term cumulative impact
score of 0. The total cumulative impact score for the 30 kGy samples was 0.52, which shows a low level
of impact on this test material. The 50 kGy sample short-term cumulative impact score was 0.14 and the
long-term cumulative impact score was 0.1. The total cumulative impact score for the 50 kGy samples
was 0.24, also showing a low impact on the tested material. These data are summarized in Table 3-5.
Table 3-5. Visual Impact Data from Historical Books
Material
Visual Changes 30 kGy

Visual Changes 50 kGy

Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Historical books
0.52
0
0.52
0.14
0.1
0.24
3.1.5.2 Technical Inspection
Technical assessments were also made of the impact of 30 kGy and 50 kGy gamma irradiation on
historical books. The 30 kGy samples showed an average color change after the short-term assessment
of 1.116. For the long-term assessment, the average color change for the 30 kGy samples increased to
1.916. The 50 kGy samples showed less of a color change than the 30 kGy samples after the short-term
assessment, with an average of 1.006. After 5 months, the average color change increased to 1.548.
These data are illustrated in Figure 3-5.
Short-term Data
¦ Long-term Data
Historical Books
30 kGy data	50 kGy data
Figure 3-5. Technical inspection data from historical books. Red dashed line indicates the smallest color
difference that the human eye can perceive, dE*ab=1
16

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3.1.6 Historical Photographs
3.1.6.1 Visual Inspection
Visual impacts were observed from gamma irradiation at 30 and 50 kGy on test samples for the long-term
assessment only. The 30 kGy samples showed a short-term cumulative impact score of 0 and a long-term
cumulative impact score of 0.1. The total impact score for the 30 kGy samples was 0.1, which showed a
low level of impact on the historical photograph test material. The 50 kGy sample short-term cumulative
impact score was 0, and the long-term cumulative impact score was 0.3. The total cumulative impact
score for the 50 kGy samples was 0.3, again showing a low impact. These data are shown in Table 3-6.
Table 3-6. Visual Impact Data from Historical Photographs

Visual Changes 30 kGy

Visual Changes 50 kGy

Material
Short-Term
Long-Term
Total
Short-Term
Long-Term
Total

Cumulative
Cumulative
Cumulative
Cumulative
Cumulative
Cumulative

Impact Score
Impact Score
Impact
Impact Score
Impact Score
Impact
Historical
photographs
0
0.1
0.1
0
0.3
0.3
3.1.6.2 Technical Inspection
Technical assessments were made of the historical photograph test samples after 30 kGy and 50 kGy
gamma irradiation. The 30 kGy samples showed an average color change after irradiation of 0.940. The
long-term assessment showed the average color change for the 30 kGy samples increased to 3.400. The
50 kGy samples showed more of a color change than the 30 kGy samples after the short-term
assessment with an average of 0.954. After 5 months, the average color change increased to 3.986.
These data are illustrated in Figure 3-6.
7
Short-term Data
¦ Long-t?rm Data
Historical Photographs
6
LU
<3
3
1
0
30 kGy data	50 kGy data
Figure 3-6. Technical inspection data from historical photographs. Red dashed line indicates the smallest color
difference that the human eye can perceive, dE*ab=1.
17

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3.2 Category 2 Material Visual and Technical Inspections
3.2.1 Historical Pastel Paintings
3.2.1.1 Visual Inspection
Visual impacts from gamma irradiation on historical pastel paintings were assessed at both the 30 and
50 kGy irradiation levels. The 30 kGy samples showed a short-term cumulative impact score of 1 and a
long-term cumulative impact score of 0.3. The total impact score for the 30 kGy samples was 1.3. This
showed a low level to moderate impact on the pastel paintings. The 50 kGy sample short-term cumulative
impact score was 0.2, and the long-term cumulative impact score was 0.2. The total cumulative impact
score for the 50 kGy samples was 0.4, showing a low impact on the tested material. These data are
summarized in Table 3-7.
Table 3-7. Visual Impact Data from Historical Pastel Paintings

Visual Changes 30 kGy

Visual Changes 50 kGy

Material
Short-Term
Long-Term
Total
Short-Term
Long-Term
Total

Cumulative
Cumulative
Cumulative
Cumulative
Cumulative
Cumulative

Impact Score
Impact Score
Impact
Impact Score
Impact Score
Impact
Historical pastel
paintings
1
0.3
1.3
0.2
0.2
0.4
3.2.1.2 Technical Inspection
Technical assessments were made of historical pastel paintings at 30 kGy and 50 kGy gamma irradiation.
The 30 kGy short-term samples showed an average color change after irradiation of 1.166. The 30 kGy
long-term assessment samples showed that the average color change increased to 2.684. The 50 kGy
short-term samples showed an average color change of 1.060, and the 50 kGy long-term assessment
samples showed an average color change of 3.060. These data are illustrated in Figure 3-7.
Historical Pastel Paintings
Short-term Data
I Long-term Data
30 kGy data
50 kGy data
Figure 3-7. Technical impact data from historical pastel paintings. Red dashed line indicates the smallest color
difference that the human eye can perceive, dE*ab=1.
18

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3.2.2 Historical Leather Items
3.2.2.1 Visual Inspection
Visual impacts of 30 and 50 kGy gamma irradiation were assessed on historical leather test samples. The
30 kGy samples showed a short-term cumulative impact score of 0.8 and a long-term cumulative impact
score of 0.1. The total impact score for the 30 kGy samples was 0.9, which shows a low level of impact on
this type of material. The 50 kGy sample short-term cumulative impact score was 0.34, and the long-term
cumulative impact score was 0.2. The total cumulative impact score for the 50 kGy samples of 0.54
showed a low impact on the tested material. These data are summarized in Table 3-8.
Table 3-8. Visual Impact Data from Historical Leather Items
Material
Visual Changes 30 kGy

Visual Changes 50 kGy

Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Historical leather
0.8
0.1
0.9
0.34
0.2
0.54
3.2.2.2 Technical Inspection
Technical assessments were made of historical leather samples at 30 kGy and 50 kGy gamma irradiation.
The 30 kGy short-term samples showed an average color change after irradiation of 1.068. The long-term
assessment color change increased to 4.498. The 50 kGy short-term color assessment showed an
average of 1.310, and the 50 kGy long-term assessment showed a color change of 4.082. These data are
illustrated in Figure 3-8.
in
to
-Q
to
*
OJ
OjO
ro
OJ
>
Historical Leather Items
Short-term Data
Long-term Data
L
30 kGy data
50 kGy data
Figure 3-8. Technical impact data from historical leather items. Red dashed line indicates the smallest color
difference that the human eye can perceive, dE*ab=1.
19

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3.2.3 Historical Wood Items
3.2.3.1 Visual Inspection
Visual impacts from gamma irradiation were observed only in the 50 kGy short-term test samples for
historical wood. The 50 kGy samples showed a short-term cumulative impact score of 0.2. All other
samples showed no visual impacts. These data are summarized in Table 3-9.
Table 3-9. Visual Impact Data from Historical Wood Items

Visual Changes 30 kGy

Visual Changes 50 kGy

Material
Short-Term
Long-Term
Total
Short-Term
Long-Term
Total

Cumulative
Cumulative
Cumulative
Cumulative
Cumulative
Cumulative

Impact Score
Impact Score
Impact
Impact Score
Impact Score
Impact
Historical wood
0
0
0
0.2
0
0.2
3.2.3.2 Technical Inspection
Technical assessments were made of historical wood items after 30 kGy and 50 kGy gamma irradiation.
The 30 kGy short-term samples showed an average color change of 3.146. The long-term samples
showed an average color change increase to 5.704. The 50 kGy short-term samples showed less of a
color change than the 30 kGy samples after irradiation, with an average of 1.804. For the long-term
samples, the average color change increased to 5.810. These data are illustrated in Figure 3-9.
Short-term Data
¦ Long-term Data
Historical Wood Items
30 kGy data	50 kGy data
Figure 3-9. Technical impact data from historical wood items. Red dashed line indicates the smallest color
difference that the human eye can perceive, dE*ab=1.
20

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3.2.4 Historical Porcelain Items
3.2.4.1 Visual Inspection
Visual impacts from gamma irradiation of historical porcelain items were observed at both the 30 and
50 kGy irradiation levels. The 30 kGy samples showed a short-term cumulative impact score of 3 and a
long-term cumulative impact score of 8.4. The total impact score for the 30 kGy samples was 11. This
shows a high level of impact on the tested porcelain material. The 50 kGy sample short-term cumulative
impact score was 4.4, and the long-term cumulative impact score was 7.8. The total cumulative impact
score for the 50 kGy samples of 12.2, again shows a significant impact on the tested material. These data
are summarized in Table 3-10.
Table 3-10. Visual Impact Data from Historical Porcelain Items
Material
Visual Changes 30 kGy

Visual Changes 50 kGy

Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Short-Term
Cumulative
Impact Score
Long-Term
Cumulative
Impact Score
Total
Cumulative
Impact
Historical
porcelain
3
8.4
11.4
4.4
7.8
12.2
3.2.4.2 Technical Inspection
Technical assessments were made of historical porcelain items at 30 kGy and 50 kGy gamma irradiation.
The 30 kGy short-term samples showed a large average color change after irradiation of 21.668. The
30 kGy long-term sample average color change decreased slightly to 19.178. The 50 kGy short-term
samples showed an average color change of 21.920. For the 50 kGy long-term samples, the average
color change slightly decreased to 18.736. These data are illustrated in Figure 3-10.
21

-------
in
10
O
-Q 16
(D
*
LU 14
<3
o 12
tuO
£ io
Q)
m 8
ro
6
4
2
0


Historical Porcelain Items
Short-term Data



¦ Long-term Data
















































































— — —

- —
30 kGy data	50 kGy data
Figure 3-10. Technical impact data from historical porcelain items. Red dashed line indicates the smallest
color difference that the human eye can perceive, dE*ab=1.
3.2.5 Historical Fabric Items
3.2.5.1 Visual Inspection
Visual impacts from gamma irradiation of the historical fabric test samples were seen only at the short-
term 30 kGy irradiation level. The impact score for the short-term 30 kGy samples was 0.7. All other tests
showed no visual impacts. These data are summarized in Table 3-11.
Table 3-11. Visual Impact Data from Historical Fabric Items

Visual Changes 30 kGy

Visual Changes 50 kGy

Material
Short-Term
Long-Term
Total
Short-Term
Long-Term
Total

Cumulative
Cumulative
Cumulative
Cumulative
Cumulative
Cumulative

Impact Score
Impact Score
Impact
Impact Score
Impact Score
Impact
Historical fabric
0.7
0
0.7
0
0
0
3.2.5.2 Technical Inspection
Technical assessments were made of historical fabric samples at 30 kGy and 50 kGy gamma irradiation.
The 30 kGy short-term samples showed an average color change of 2.062. The long-term assessment
showed an average color change of 4.246. The short-term assessment 50 kGy samples showed an
average color change of 1.726. For the long-term 50 kGy assessment samples, average color change
increased to 2.694. These data are illustrated in Figure 3-11.
22

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Short-term Data
¦ Long-term Data
Historical Fabric Items
30 kGy data	50 kGy data
Figure 3-11. Technical impact data from historical fabric items. Red dashed line indicates the smallest color
difference that the human eye can perceive, dE*ab=1.
3.2.6 Historical Metal Items
3.2.6.1 Visual Inspection
No visual impacts were observed from gamma irradiation of the historical metal samples at both the 30
and 50 kGy irradiation levels. These data are summarized in Table 3-12.
Table 3-12. Visual Impact Data from Historical Metal Items

Visual Changes 30 kGy

Visual Changes 50 kGy

Material
Short-Term
Long-Term
Total
Short-Term
Long-Term
Total

Cumulative
Cumulative
Cumulative
Cumulative
Cumulative
Cumulative

Impact Score
Impact Score
Impact
Impact Score
Impact Score
Impact
Historical metal
0
0
0
0
0
0
3.2.6.2 Technical Inspection
Technical assessments were made of historical metal surrogates at 30 kGy and 50 kGy gamma
irradiation. The 30 kGy short-term samples showed an average color change after irradiation of 1.334,
and the long-term assessment showed an average color change of 3.602. The 50 kGy short-term
samples showed an average color change of 1.768, and the long-term assessment showed a color
change of 3.956. These data are illustrated in Figure 3-12.
23

-------
Short-term Data
¦ Long-term Data
Historical Metal Items
30 kGy data	50 kGy data
Figure 3-12. Technical impact data from historical metal items. Red dashed line indicates the smallest color
difference that the human eye can perceive, dE*ab=1.
3.3 Dosage and Sterilization Verification
3.3.1	Dosage Verification Data
Each irradiation level was checked with dosimeters to verify the gamma irradiation dosage. The
acceptance criteria for the dosimeter data was ± 20% of target value. The 30 kGy samples received a
measured dosage of 33.3 kGy. The 50 kGy samples received a measured dosage of 53.3 kGy. The
certificates for irradiation are provided in Appendix D.
3.3.2	Sterilization Verification Data
Bis for the 30 kGy gamma irradiation samples showed no growth for all samples with the exception of the
B. atrophaeus Bl in the historical documents sample, which showed growth. All positive controls showed
growth and all negative controls showed no growth for all samples. The sample sheet for the 30 kGy data
is given in Appendix E.
Three Bis for B. atrophaeus for the 50 kGy dose showed growth. The historical wood, light hue
monochromatic pigment oil painting, and historical pastel painting sample Bis all showed growth. The
remaining samples showed no growth. All positive controls showed growth and all negative controls
showed no growth.
One Bl for the leather samples showed growth for B. pumilus. The remaining sample Bis showed no
growth. All positive controls showed growth and all negative controls showed no growth. The 50 kGy data
can be found in Appendix E.
24

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The Bis used in this project were analyzed following irradiation by placing the Bl in growth media and
examining for growth or no growth. This is a quantitative measurement and would only take one spore to
turn the growh media turbid resulting in a positive designation. The 30 and 50 kGy doses were chosen
based on previous research. In the event that irradiation was being considered for inactivation of B.
anthracis on valuable objects, environmental samples should be collected pre and post exposure to verify
the item has been decontaminated.
25

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4 Quality Assurance
The objective of this study was to assess the impact of decontamination with gamma irradiation on
museum-quality materials at conditions known to be effective against biological threats. The data quality
indicators (DQIs) address this impact using visual and technical inspections to assess the potential
damage and loss in value. The following measurements were deemed critical to accomplishing part or all
of the project objectives (see Table 4-1):
•	Gamma irradiation dose
•	Material inspections (visual and technical assessments)
The following non-critical measurements were also required to ensure the quality of project data:
•	Temperature and %RH values for the environmental chamber used for conditioning and storage
of coupons
•	Mass of sample boxes sent to gamma irradiation facility
•	Sterility assurance checks using Bis
In addition to the spectrophotometer used for the technical assessments, standard laboratory equipment
such as balances and RH/temperature probes were routinely monitored for proper performance.
Calibration of instruments was done at the frequency shown in Table 4-2. Any deficiencies were noted,
and the instrument was adjusted to meet calibration tolerances and recalibrated within 24 hours. If
tolerances were not met after recalibration, additional corrective action was taken that possibly included
recalibration or replacement of the equipment.
Standard commercial spore strips (i.e., Bis) were analyzed for growth/no growth to verify sporicidal
efficacy. All Bis were maintained in their sterile Tyvek/Mylar envelopes and placed into the sample boxes
before they were sent to be gamma-irradiated. After the samples returned from gamma-radiation the Bis
were analyzed for growth/no growth.
Table 4-1. Data Quality Indicators for Critical Measurements
Measurement
Parameter
Analysis Method
Accuracy
Precision/Repeatability
Completeness
%
Gamma
irradiation dose
Dosimeter
±20% of target
value
NA
100%
Visual
assessments
Visual assessment
checklist
NA
Individual human eye
perception threshold/
performed by same analyst
100%
Technical
assessments
Spectrophotometry
0.25 AE*aba, D65b
AE*ab, D65 within 0.05
standard deviation
100%
aAE*ab: color difference measured with standard illuminant D65; averaged for 12 British Ceramic Research
Association series II color tiles compared to values measured with master body at 23 °C.
bWhite calibration plate measured 30 times at 10-second intervals after white calibration is performed.
26

-------
Table 4-2. Test Equipment Calibration Schedule
Equipment
Calibration
Frequency
Spectrophotometer
Calibrated using white calibration plate CM-A153
Prior to each test
Vaisala probe
(RH, temperature)
Calibrated using salt solution wells (RH) and by comparison to
NIST-traceable thermocouple (temperature) via standard
laboratory procedures
Yearly
Balance
Calibrated using NIST-traceable standards
Yearly
aNIST: National Institute of Standards and Technology
27

-------
5 Conclusions
5.1	Category 1 Materials (Priority Materials)
All category 1 materials showed effects from gamma irradiation at both 30 and 50 kGy. Visual
assessments for the historical oil paintings and historical documents showed an increased impact from
the short-term assessments to the long-term assessments. The light hue monochromatic pigment oil
painting samples showed no visual impact in the short-term 30 kGy assessment, but changes were
observed in the long-term assessment. The 50 kGy data showed an impact in the short-term assessment,
which increased in the long-term assessment. The dark hue monochromatic pigment oil painting samples
showed an impact on the 30 kGy short-term samples, but no impact on the long-term samples.The 50
kGy samples showed no short-term impacts, but a slight impact after the long-term assessments. The
results for historical paintings were similar - samples exposed to 30kGy and 50 kGy were minimally to
moderately affected short term, but shown significant impacts long-term. The historical books showed
both short- and long-term changes. The historical photographs showed no short-term impact at both 30
and 50 kGy, but long-term effects were observed.
The technical assessments showed short- and long-term impacts for all category 1 samples. An
increased color change was noted for all samples from the short-term assessment to the long-term
assessment. The short-term 50 kGy samples showed a greater impact than the 30 kGy samples except
for the historical documents and the historical books. The historical document short-term 30 kGy samples
showed a greater impact than the 50 kGy samples. On the other hand, the long-term 50 kGy historical
document samples showed a greater impact than the 30 kGy samples. The historical book 30 kGy
samples showed more impact than the 50 kGy samples in both the short- and long-term assessments.
5.2	Category 2 Materials (Secondary Materials)
The visual assessment showed some impact for all samples except the metal samples, which showed no
changes. The fabric samples only showed a visual impact for the 30 kGy short-term samples. The wood
samples showed a visual impact for the short-term 50 kGy samples only. The remaining samples, pastel
painting, leather, and porcelain showed impacts for both 30 and 50 kGy in the short- and long-term
assessments.
The technical assessments showed impacts for all short- and long-term samples. An increase was
observed for all samples from the short-term assessment to the long-term assessment except for the
historic porcelain, which showed a slight decrease from the short-term to the long-term assessment. The
pastel paintings, wood, and fabric all showed a higher short-term impact for 30 kGy than the 50 kGy
samples. The long-term assessment impacts for wood samples showed the 50 kGy samples had a
greater impact than the 30 kGy samples. The long-term 30 kGy samples for the fabric showed a greater
impact than the 50 kGy samples. The analysis of historical pastel paintings and leather samples showed
very minimal short-term impact at both 30 and 50 kGy, but long-term effects were observed. The
porcelain matrix was material most dramatically affected by gamma-irradiation with average short- and
long-term impacts over 15 to 20 times higher than the human eye perception threshold. The cumulative
impacts of gamma-irradiation were predominantly driven by changes in color, hue and saturation: lighter
but less saturated colors were mostly observed, the predominant post-exposure change in hue was
yellowing (Appendix C). - see Tables 3-1 through 3-4 for details).
28

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These experimental results provide an insight into which materials are most at risk for damage from the
use of irradiation. Some of the materials experienced degradation following irradiation. In some
instances, additional degradation occurred as a function of time. A different decontamination approach
should be used for these items that are impacted by irradiation.
5.3 Biological Indicator Results
Bis were placed with each of the materials that were irradiated. Bis for the 30 kGy gamma irradiation
samples showed no growth for all samples with the exception of the B. atrophaeus Bl in the historical
documents sample, which showed growth.
Three Bis for B. atrophaeus for the 50 kGy dose showed growth. The historical wood, light hue
monochromatic pigment oil painting, and historical pastel painting sample Bis all showed growth. The
remaining samples showed no growth.
One Bl for the leather samples showed growth forB. pumilus. The remaining sample Bis showed no
growth.
The Bis used in this project were analyzed following irradiation by placing the Bl in growth media and
examining for growth or no growth. This is a quantitative measurement and would only take one spore to
turn the growh media turbid resulting in a positive designation. The 30 and 50 kGy doses were chosen
based on previous research. In the event that irradiation was being considered for inactivation of B.
anthracis on valuable objects, environmental samples should be collected pre and post exposure to verify
the item has been decontaminated.
29

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6 References
[1]	da Silva M, Moraes AML, Nishikawa MM, Gatti MJA, Vallim de Alencar MA, Brandao LE, Nobrega A.
(2006) Inactivation of fungi from deteriorated paper materials by radiation. Int Biodeter Biodegr, 57:163-167.
[2]	Geba M, Vlad AM, Ciovica S. (2008) Gamma irradiation for the preservation of historical papers: a
critical evaluation. Cell Chem Techno!, 42:97-102.
[3]	Magaudda G. (2004) The recovery of biodeteriorated books and archive documents through gamma
radiation: some considerations on the results achieved. J Cult Herit, 5:113-118.
[4]	Sterigenics (2014). Sterilization technology. Gamma Irradiation, http://www.sterigenics.com/
Sterilizath inoloqies/Gamma Irradiation.php, last accessed February 29, 2016.
[5]	Sterigenics (2014). Sterilization technology. SteriPro® Laboratories and Consulting Services.
http://www.steriaeiiics.com/Customer Solutions/SteriProzzr Laboratories and Consulting Services.ohp.
last accessed February 29, 2016.
[6]	Gregory B. Knudson (2011). Selection of doses for irradiation of historical materials for WA 60.
Personal correspondence with Dr. Shannon Serre (WACOR).
[7]	ANSI/AAMI/ISO (2006). Sterilization of health care products—Radiation—Part 3: Guidance on
dosimetric aspects. American National Standard/Association for the Advancement of Medical
Instrumentation/International Standard Organization. ANSI/AAMI/ISO 11137-3:2006
http://marketplace.aami.org/eseries/scriptooiiteiit/docs/Preview%20Files%5C11137030604preview.pdf
[8]	US Department of Defense, Review Committee Report: Inadvertent Shipment of Live Bacillus
anthracis Spores by DoD, http://www.defense.gOv/Portals/1/featyres/2015/0615 lab-stats/Review-
Committee-Report-Final.pdf. July 13, 2015, accessed October 17, 2016.
[9]	Konica Minolta Sensing, Spectra Magic NX Navigation. Precise Color Communication Manual. Spectra
Magic NX Manual, 2002.
30

-------
Appendix A: Biological Indicator Certificates of Analysis
~
MesaLabs
CERTIFICATE OF ANALYSIS
MESA BIOLOGICAL INDICATORS
Bacillus atrophaeus Spore Strips - Recommended for use in evaluating Dry Heat
or Ethylene Oxide gas sterilization processes.
This document certifies that the biological indicators for this lot meet Mesa Labs'
quality control specifications, AAM1/ISO 11138:2006 parts 1 & 2 and suggested
performance parameters published in the current United States Pharmacopeia.
Thomas Halpenny
Quality Assurance Specialist
Mesa Labs
Manufacture: 07JAN2015
Release: 27JAjN2015
Performance Data for Lot # 11632111 Batch 321GB Expiration Date 01/2017
Organism: Bacillus atrophaeus	ATCC" No. 9372
Nominal Population 3-8 * 10 CFU*/L5"x 0-25'stnp
iNunniiui rupuiauun	minutes (600 mg EtO/L, 54"C, 60% RH- This accuracy
Deio Value** 3.6	shall not exceed +/- 0.5)
D,m Value** 1.1	minutes (Dry Heat, 16CC- This accuracy
shall not exceed +/- 0.2)
°C; approximate
Z-value*** 33.8
4 Colony Forming Units
* • Determined on primary spore crop using paper strips in glnssme envelopes, Spearman-Kaiber method The D-value is reproducible
only under the exact conditions under which it was determined The user would not necessarily obtain the same results
Therefore, the user would need to determine the suitability for its particular use
••• See reverse side
Resistance Characteristics: (Based on US Pharmacopeia Calculations)
AGENT	CONDITIONS	SURVIVES	KILLED
Ethylene Oxide 54 + l°C, 600 + 30 mg/L,	16.5 tnin	38.1 min.
60 ± 10% RH
Dry Heat	160 + 2°C	5.0 min.	11-6 min.
Purity: No evidence of contaminants using standard plate count techniques.
Incubation: 7 days in soybean-casein digest broth at a temperature of 30 - jS'C
Storage: 15 - 27"C (60 - 80°F), 30 - 70% RH, away from sterilizing agents, direct sunlight and all
other forms of UV light. (Do Not Refrigerate).
Disposal: Do not use after expiration date. Sterilize all cultures before discarding.
ATCC K • Regional Trademark of the American Type Culture Collection
09/21/12
Mesa Laboratories Inc. Omaha Manufacturing Facility 8607 Park Drive Omaha, NE 68127 USA
bi-support@mesalabs com (303) 987-8000 FAX (402) 593-0921 www.mesalabs.com
Our Quality System is Registered to ISO 13485 Standards
31

-------
E
MesaLabs
CERTIFICATE OF ANALYSIS
MESA BIOLOGICAL INDICATORS
Bacillus pumilus Spore Strips - Recommended for use in evaluating gamma
radiation sterilization processes.
This document certifies that the biological indicators for this lot meets
Mesa Labs' quality control specifications, and suggested parameters
published in the current United States Pharmacopeia.
		
Thomas Halpenny
Quality Assurance Specialist
Mesa Labs
Manufacture: 030CT2014
Release: 080CT20I4
Performance Data for Lot # 716707
Batch 70P
Expiration Date 10/2016
Organism: Bacillus pumilus
ATCC"" No. 27142
Nominal Population 1.9 x 10
Devalue** 0.12
CFU* /1.5" x 0.25" strip
Mrads (1 Mrad = 10 kGry)
Pour plate dilutions indicate less than IVaBacillus sublilis
* Colony Forming Units
•* Determined at time of manufacture, using Linear Regression method The D-valuc is rcpioducible only under the
exact conditions under which it was determined The user would not necessarily obtain the same results Therefore,
the user would need to determine the suitability for its particular use
Purity: No evidence of contaminants using standard plate count techniques.
Incubation: 7 days in soybean-casein digest broth at a temperature of 30 - 3 SC.
Storage: 60 - 80°F (15 - 27°C), 30 - 70% RH, away from sterilizing agents, direct sunlight, and
all other forms of UV light. (Do Not Refrigerate).
Disposal: Do not use after expiration date. Sterilize all cultures before discarding.
ATCC«»¦ Rc^ittcred Tndcmuk of the American Type Culture Collection
09/24/12
Mesa Laboratories I nc Omaha Manufacturing Facility 8607 Park Drive Omaha, NE 68127 USA
bi-support@mesalabs.com (303)987-8000 FAX (402) 593-0921 www.mesalabs.com
Our Quality System is Registered to ISO 13485 Standards
32

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Appendix B: Visual Impact Data Sheets
B-1: Short-Term Data
Check List for the Basic Visual Assessments Historical Oil Painting 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors


81 visual assessment after sterilization (performed:5/29/2015, by: Josh Nardin)


Historical
Oil
Painting
1
30 kGy
5/18/2015
WA60-OPS-GI-30kGy-A
0
0
0
0.5
0.5
top layer
thinning
0
0
Cumulative impact for 1st assessment sample A:1
Historical
Oil
Painting
2
30 kGy
5/18/2015
WA60-OPS-GI-30kGy-B
0
0
0
0
0.5
corner
thinning
0
0
Cumulative impact for 1s assessment sample B: 0.5
Historical
Oil
Painting
3
30 kGy
5/18/2015
WA60-OPS-GI-30kGy-C
0.5
darker
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0.5
Historical
Oil
Painting
4
30 kGy
5/18/2015
WA60-OPS-GI-30kGy-D
0
0
0.5
lighter
0
0
0
0
Cumulative impact for 1s assessment sample D: 0.5
Historical
Oil
Painting
5
30 kGy
5/18/2015
WA60-OPS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1st assessment (n=5): 2.5
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
33

-------
Check List for the Basic Visual Assessments Historical Oil Painting 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed:6/3/20015, by: Josh Nardin)
Historical
Oil
Painting
1
50 kGy
5/17/2015
WA60-OPS-GI-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample A: 0
Historical
Oil
Painting
2
50 kGy
5/17/2015
WA60-OPS-GI-50kGy-B
0
0
0.5
lighter red
coloration
0
0
0
0
Cumulative impact for 1s assessment sample B: 0.5
Historical
Oil
Painting
3
50 kGy
5/17/2015
WA60-OPS-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0
Historical
Oil
Painting
4
50 kGy
5/17/2015
WA60-OPS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Oil
Painting
5
50 kGy
5/17/2015
WA60-OPS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1s assessment (n=5): 0.5
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
34

-------
Check List for the Basic Visual Assessments Light Hue Monochromatic Pigment Oil Painting 30
kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed:5/29/2015, by: Josh Nardin)
Light Hue
Monochromatic
Pigment Oil
Painting
1
30 kGy
5/18/2015
WA60-OLT-GI-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample A: 0
Light Hue
Monochromatic
Pigment Oil
Painting
2
30 kGy
5/18/2015
WA60-OLT-GI-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample B: 0
Light Hue
Monochromatic
Pigment Oil
Painting
3
30 kGy
5/18/2015
WA60-OLT-GI-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0
Light Hue
Monochromatic
Pigment Oil
Painting
4
30 kGy
5/18/2015
WA60-OLT-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Light Hue
Monochromatic
Pigment Oil
Painting
5
30 kGy
5/18/2015
WA60-OLT-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1s assessment (n=5): 0
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
35

-------
Check List for the Basic Visual Assessments Light Hue Monochromatic Pigment Oil Painting 50
kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

1st
visual assessment after sterilization (performed: 6/4/2015, by Josh Nardin)


Light Hue
Monochromatic
Pigment Oil
Painting
1
50 kGy
5/17/2015
WA60-OLT-GI-50kGy-A
0
0
0.2
slightly
darker
0
0
0
0
Cumulative impact for 1st assessment sample A: 0.2
Light Hue
Monochromatic
Pigment Oil
Painting
2
50 kGy
5/17/2015
WA60-OLT-GI-50kGy-B
0
0
0.5
slightly
darker
0
0
0
0
Cumulative impact for 1st assessment sample B: 0.5
Light Hue
Monochromatic
Pigment Oil
Painting
3
50 kGy
5/17/2015
WA60-OLT-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 1st assessment sample C: 0
Light Hue
Monochromatic
Pigment Oil
Painting
4
50 kGy
5/17/2015
WA60-OLT-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1st assessment sample D: 0
Light Hue
Monochromatic
Pigment Oil
Painting
5
50 kGy
5/17/2015
WA60-OLT-GI-50kGy-E
0
0
0.2
slightly
darker
0
0
0
0
Cumulative impact for 1st assessment sample E: 0.2

Cumulative impact for 1s assessment (n=5): 0.9
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, <0.5: ultra-low but perceptible change, 0-No change; relative change
as compared to the baseline (pre-exposure) condition
36

-------
Check List for the Basic Visual Assessments Light Dark Monochromatic Pigment Oil Painting 30
kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed: 6/3/2015, by: Josh Nardin)
Dark Hue
Monochromatic
Pigment Oil
Painting
1
30 kGy
5/18/2015
WA60-ODT-GI-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 1st assessment sample A: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
2
30 kGy
5/18/2015
WA60-ODT-GI-30kGy-B
1 more
brown
0
1
lighter
0
0
0
0
Cumulative impact for 1st assessment sample B: 2
Dark Hue
Monochromatic
Pigment Oil
Painting
3
30 kGy
5/18/2015
WA60-ODT-GI-30kGy-C
1 more
brown
0
1
lighter
0
0
0
0
Cumulative impact for 1st assessment sample C: 2
Dark Hue
Monochromatic
Pigment Oil
Painting
4
30 kGy
5/18/2015
WA60-ODT-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1st assessment sample D: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
5
30 kGy
5/18/2015
WA60-ODT-GI-30kGy-E
0.5
slightly
browner
0
0.5
slightly
lighter
0
0
0
0
Cumulative impact for 1s assessment sample E:1

Cumulative impact for 1st assessment (n=5): 5
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
37

-------
Check List for the Basic Visual Assessments Light Dark Monochromatic Pigment Oil Painting 50
kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

1st
visual assessment after sterilization (performed:6/3/2015, by Josh Nardin)


Dark Hue
Monochromatic
Pigment Oil
Painting
1
50 kGy
5/17/2015
WA60-ODT-GI-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 1st assessment sample A: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
2
50 kGy
5/17/2015
WA60-ODT-GI-50kGy-B
0
0
0
0
0
0
0
Cumulative impact for 1st assessment sample B: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
3
50 kGy
5/17/2015
WA60-ODT-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 1st assessment sample C: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
4
50 kGy
5/17/2015
WA60-ODT-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1st assessment sample D: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
5
50 kGy
5/17/2015
WA60-ODT-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1st assessment sample E: 0

Cumulative impact for 1st assessment (n=5): 0
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1 -Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
38

-------
Check List for the Basic Visual Assessments Historical Documents 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed: 5/29/2015, by: Josh Nardin)
Historical
Document
1
30 kGy
5/18/2015
WA60-DS-GI-30kGy-A
0
0
0.1
slightly
lighter
backing
0
0
0
0
Cumulative impact for 1s assessment sample A: 0.1
Historical
Document
2
30 kGy
5/18/2015
WA60-DS-GI-30kGy-B
1 less
yellow
more
gray
0
1
backing
duller
0
0
0
0
Cumulative impact for 1s assessment sample B: 2
Historical
Document
3
30 kGy
5/18/2015
WA60-DS-GI-30kGy-C
0
0
1
backing
and
script
slightly
lighter
0
0
0
0
Cumulative impact for 1s assessment sample C:1
Historical
Document
4
30 kGy
5/18/2015
WA60-DS-GI-30kGy-D
1
more
brown
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D:1
Historical
Document
5
30 kGy
5/18/2015
WA60-DS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1st assessment (n=5): 4.1
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1 -Low
level of change, 0.5-Very low level of change, <0.5 -ultra-low but perceptible change, 0-No change; relative
change as compared to the baseline (pre-exposure) condition
39

-------
Check List for the Basic Visual Assessments Historical Documents 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

1s1
visual assessment after sterilization (performed: 5/29/2015, by: Josh Nardin)


Historical
Document
1
50 kGy
5/17/2015
WA60-DS-GI-50kGy-A
0
0
0.2
backing
slightly
darker
0
0
0
0
Cumulative impact for 1s assessment sample A: 0.2
Historical
Document
2
50 kGy
5/17/2015
WA60-DS-GI-50kGy-B
0
0
0.5
lighter
0
0
0
0
Cumulative impact for 1s assessment sample B: 0.5
Historical
Document
3
50 kGy
5/17/2015
WA60-DS-GI-50kGy-C
0
0
0.2
backing
darker
0
0
0
0
Cumulative impact for 1s assessment sample C: 0.2
Historical
Document
4
50 kGy
5/17/2015
WA60-DS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Document
5
50 kGy
5/17/2015
WA60-DS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0.0

Cumulative impact for 1st assessment (n=5): 0.9
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1 -Low
level of change, 0.5-Very low level of change, <0.5 -ultra-low but perceptible change, 0-No change; relative
change as compared to the baseline (pre-exposure) condition
40

-------
Check List for the Basic Visual Assessments Historical Books 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed: 6/03/2015 , by: Josh Nardin)
Historical
Book
1
30 kGy
5/18/2015
WA60-BS-GI-30kGy-A
0
0
0.2
barely
darker
0
0
0
0
Cumulative impact for 1s assessment sample A: 0.2
Historical
Book
2
30 kGy
5/18/2015
WA60-BS-GI-30kGy-B
0.5
yellower
backing
0
0.5
brighter
0
0
0
0
Cumulative impact for 1s assessment sample B: 1
Historical
Book
3
30 kGy
5/18/2015
WA60-BS-GI-30kGy-C
0.5
more
yellow
0
0.5
brighter
0
0
0
0
Cumulative impact for 1s assessment sample C: 1
Historical
Book
4
30 kGy
5/18/2015
WA60-BS-GI-30kGy-D
0
0
0.2
slightly
duller
0
0
0
0
Cumulative impact for 1s assessment sample D: 0.2
Historical
Book
5
30 kGy
5/18/2015
WA60-BS-GI-30kGy-E
0
0
0.2
brighter
0
0
0
0
Cumulative impact for 1s assessment sample E: 0.2

Cumulative impact for 1st assessment (n=5): 2.6
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1 -Low
level of change, 0.5-Very low level of change, <0.5 -ultra-low but perceptible change, 0-No change; relative
change as compared to the baseline (pre-exposure) condition
41

-------
Check List for the Basic Visual Assessments Historical Books 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

1
51 visual assessment after sterilization (performed: 6/03/2015, by: Josh Nardin)


Historical
Book
1
50 kGy
5/17/2015
WA60-BS-GI-50kGy-A
0.5
slightly
darker
brown
0
0
0
0
0
0
Cumulative impact for 1s assessment sample A: 0.5
Historical
Book
2
50 kGy
5/17/2015
WA60-BS-GI-50kGy-B
0
0
0.1
less
yellow
0
0
0
0
Cumulative impact for 1s assessment sample B: 0.1
Historical
Book
3
50 kGy
5/17/2015
WA60-BS-GI-50kGy-C
0
0
0.1
slightly
lighter
0
0
0
0
Cumulative impact for 1s assessment sample C: 0.1
Historical
Book
4
50 kGy
5/17/2015
WA60-BS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Book
5
50 kGy
5/17/2015
WA60-BS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1st assessment (n=5): 0.7
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1 -Low
level of change, 0.5-Very low level of change, <0.5 -ultra-low but perceptible change, 0-No change; relative
change as compared to the baseline (pre-exposure) condition
42

-------
Check List for the Basic Visual Assessments Historical Photographs 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

1st
visual assessment after sterilization (performed: 5/29/2015, by: Josh Nardin)


Historical
Photograph
1
30 kGy
5/18/2015
WA60-PS-GI-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample A: 0
Historical
Photograph
2
30 kGy
5/18/2015
WA60-PS-GI-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample B: 0
Historical
Photograph
3
30 kGy
5/18/2015
WA60-PS-GI-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0
Historical
Photograph
4
30 kGy
5/18/2015
WA60-PS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Photograph
5
30 kGy
5/18/2015
WA60-PS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1s assessment (n=5): 0
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
43

-------
Check List for the Basic Visual Assessments Historical Photographs 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

1s
visual assessment after sterilization (performed: 6/3/2015, by Josh Nardin)


Historical
Photograph
1
50 kGy
5/17/2015
WA60-PS-GI-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample A: 0
Historical
Photograph
2
50 kGy
5/17/2015
WA60-PS-GI-50kGy-B
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample B: 0
Historical
Photograph
3
50 kGy
5/17/2015
WA60-PS-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0
Historical
Photograph
4
50 kGy
5/17/2015
WA60-PS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Photograph
5
50 kGy
5/17/2015
WA60-PS-GI -50 kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1s assessment (n=5): 0
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
44

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Check List for the Basic Visual Assessments Historical Pastel Paintings 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

1
st visual assessment after sterilization (performed:5/29/2015, by: Josh Nardin)


Historical
Pastel
Painting
1
30 kGy
5/18/2015
WA60-PPS-GI-30kGy-A
0
0
0.5
0
1.5 Top
layer
thinning
0
0
Cumulative impact for 1st assessment sample A:2
Historical
Pastel
Painting
2
30 kGy
5/18/2015
WA60-PPS-GI-30kGy-B
0
0
0
0
1.5 Top
layer
thinning
0
0
Cumulative impact for 1s assessment sample B:1.5
Historical
Pastel
Painting
3
30 kGy
5/18/2015
WA60-PPS-GI-30kGy-C
0
0
0
0
0.5
0
0
Cumulative impact for 1s assessment sample C:0.5
Historical
Pastel
Painting
4
30 kGy
5/18/2015
WA60-PPS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D:0
Historical
Pastel
Painting
5
30 kGy
5/18/2015
WA60-PPS-GI-30kGy-E
0
0
0
0
0.5
0
0
Cumulative impact for 1s assessment sample E:0.5

Cumulative impact for 1st assessment (n=5): 4.5
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
45

-------
Check List for the Basic Visual Assessments Historical Pastel Paintings 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed:6/3/2015, by: Josh Nardin)
Historical
Pastel
Painting
1
50 kGy
5/17/2015
WA60-PPS-GI-50kGy-A
0
0
0.2
0
0.2
Thinning
0
0
Cumulative impact for 1s assessment sample A: 0.4
Historical
Pastel
Painting
2
50 kGy
5/17/2015
WA60-PPS-GI-50kGy-B
0
0
0
0
0.2
Thinning
0
0
Cumulative impact for 1s assessment sample B: 0.2
Historical
Pastel
Painting
3
50 kGy
5/17/2015
WA60-PPS-GI-50kGy-C
0
0
0
0
0.2
0
0
Cumulative impact for 1s assessment sample C: 0.2
Historical
Pastel
Painting
4
50 kGy
5/17/2015
WA60-PPS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Pastel
Painting
5
50 kGy
5/17/2015
WA60-PPS-GI-50kGy-E
0
0
0
0
0.2
Thinning
0
0
Cumulative impact for 1s assessment sample E: 0.2

Cumulative impact for 1s assessment (n=5): 1
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1 -Low
level of change, 0.5-Very low level of change, <0.5 -ultra-low but perceptible change, 0-No change; relative
change as compared to the baseline (pre-exposure) condition
46

-------
Check List for the Basic Visual Assessments Historical Leather Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed:5/29/2015, by: Josh Nardin)
Historical
Leather
1
30 kGy
5/18/2015
WA60-LS-GI-30kGy-A
1 lighter
yellow
0
0.5
lighter
0
0
0
0
Cumulative impact for 1s assessment sample A:1.5
Historical
Leather
2
30 kGy
5/18/2015
WA60-LS-GI-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample B: 0
Historical
Leather
3
30 kGy
5/18/2015
WA60-LS-GI-30kGy-C
0.5
yellower
0
0.5
lighter
0
0
0
0
Cumulative impact for 1s assessment sample C:1
Historical
Leather
4
30 kGy
5/18/2015
WA60-LS-GI-30kGy-D
0
0
0.5
lighter
back
0
0
0
0
Cumulative impact for 1s assessment sample D: 0.5
Historical
Leather
5
30 kGy
5/18/2015
WA60-LS-GI-30kGy-E
0.5
lighter
0
0.5
lighter
front
0
0
0
0
Cumulative impact for 1st assessment sample E:1

Cumulative impact for 1s assessment (n=5): 4
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
47

-------
Check List for the Basic Visual Assessments Historical Leather Items 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed: 6/3/2015, by: Josh Nardin)
Historical
Leather
1
50 kGy
5/17/2015
WA60-LS-GI-50kGy-A
0
0
0
0
0.5
thinning
0
0
Cumulative impact for 1s assessment sample A: 0.5
Historical
Leather
2
50 kGy
5/17/2015
WA60-LS-GI-50kGy-B
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample B: 0
Historical
Leather
3
50 kGy
5/17/2015
WA60-LS-GI-50kGy-C
0.2
darker
patches
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0.2
Historical
Leather
4
50 kGy
5/17/2015
WA60-LS-GI-50kGy-D
0
0
0
0
0.5
thinning
0
0
Cumulative impact for 1s assessment sample D: 0.5
Historical
Leather
5
50 kGy
5/17/2015
WA60-LS-GI-50kGy-E
0.5
strip of
more
orange
color
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0.5

Cumulative impact for 1st assessment (n=5): 1.7
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1 -Low
level of change, 0.5-Very low level of change, <0.5 -ultra-low but perceptible change, 0-No change; relative
change as compared to the baseline (pre-exposure) condition
48

-------
Check List for the Basic Visual Assessments Historical Wood Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

1s1
visual assessment after sterilization (performed: 5/29/2015, by: Josh Nardin)


Historical
Wood
1
30 kGy
5/18/2015
WA60-WS-GI-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample A: 0
Historical
Wood
2
30 kGy
5/18/2015
WA60-WS-GI-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample B: 0
Historical
Wood
3
30 kGy
5/18/2015
WA60-WS-GI-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0
Historical
Wood
4
30 kGy
5/18/2015
WA60-WS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Wood
5
30 kGy
5/18/2015
WA60-WS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1s assessment (n=5): 0
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
49

-------
Check List for the Basic Visual Assessments Historical Wood Items 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed: 6/4/15, by: Josh Nardin)
Historical
Wood
1
50 kGy
5/17/2015
WA60-WS-GI-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample A: 0
Historical
Wood
2
50 kGy
5/17/2015
WA60-WS-GI-50kGy-B
0.5
shine
0
0
0
0
0
0
Cumulative impact for 1s assessment sample B: 0.5
Historical
Wood
3
50 kGy
5/17/2015
WA60-WS-GI-50kGy-C
0.5
shine
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0.5
Historical
Wood
4
50 kGy
5/17/2015
WA60-WS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Wood
5
50 kGy
5/17/2015
WA60-WS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1s assessment (n=5): 1
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
50

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Check List for the Basic Visual Assessments Historical Porcelain Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed: 5/29/2015, by: Josh Nardin)
Historical
Porcelain
1
30 kGy
5/18/2015
WA60-PBS-GI-30kGy-A
3
turned
greenish
0
0
0
0
0
0
Cumulative impact for 1s assessment sample A: 3
Historical
Porcelain
2
30 kGy
5/18/2015
WA60-PBS-GI-30kGy-B
3
0
0
0
0
0
0
Cumulative impact for 1s assessment sample B: 3
Historical
Porcelain
3
30 kGy
5/18/2015
WA60-PBS-GI-30kGy-C
3
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 3
Historical
Porcelain
4
30 kGy
5/18/2015
WA60-PBS-GI-30kGy-D
3
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 3
Historical
Porcelain
5
30 kGy
5/18/2015
WA60-PBS-GI-30kGy-E
3
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 3

Cumulative impact for 1s assessment (n=5): 15
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
51

-------
Check List for the Basic Visual Assessments Historical Porcelain Items 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed:6/4/2015, by: Josh Nardin)
Historical
Porcelain
1
50 kGy
5/17/2015
WA60-PBS-GI-50kGy-A
2
yellow/
green
0
2
darker
0
0
0
0
Cumulative impact for 1s assessment sample A: 4
Historical
Porcelain
2
50 kGy
5/17/2015
WA60-PBS-GI-50kGy-B
2.5
yellow/
green
0
2
darker
0
0
0
0
Cumulative impact for 1s assessment sample B: 4.5
Historical
Porcelain
3
50 kGy
5/17/2015
WA60-PBS-GI-50kGy-C
3
yellow/
green
0
2.5
darker
0
0
0
0
Cumulative impact for 1s assessment sample C: 5.5
Historical
Porcelain
4
50 kGy
5/17/2015
WA60-PBS-GI-50kGy-D
2
yellow/
green
0
1
darker
0
0
0
0
Cumulative impact for 1s assessment sample D: 3
Historical
Porcelain
5
50 kGy
5/17/2015
WA60-PBS-GI-50kGy-E
3
yellow/
green
0
2
darker
0
0
0
0
Cumulative impact for 1s assessment sample E: 5

Cumulative impact for 1s assessment (n=5): 22
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
52

-------
Check List for the Basic Visual Assessments Historical Fabric Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

1
81 visual assessment after sterilization (performed:5/29/2015, by: Josh Nardin)


Historical
Fabric
1
30 kGy
5/18/2015
WA60-FS-GI-30kGy-A
1 more
purple
0
1
darker
0
0
0
0
Cumulative impact for 1s assessment sample A: 2
Historical
Fabric
2
30 kGy
5/18/2015
WA60-FS-GI-30kGy-B
0.5
more
purple
0
0.5
slightly
darker
0
0
0
0
Cumulative impact for 1st assessment sample B:1
Historical
Fabric
3
30 kGy
5/18/2015
WA60-FS-GI-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0
Historical
Fabric
4
30 kGy
5/18/2015
WA60-FS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Fabric
5
30 kGy
5/18/2015
WA60-FS-GI-30kGy-E
0.5
slightly
deeper
gold
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0.5

Cumulative impact for 1st assessment (n=5): 3.5
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
53

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Check List for the Basic Visual Assessments Historical Fabric Items 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

1
81 visual assessment after sterilization (performed:6/3/2015, by Josh Nardin)


Historical
Fabric
1
50 kGy
5/17/2015
WA60-FS-GI-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample A: 0
Historical
Fabric
2
50 kGy
5/17/2015
WA60-FS-GI-50kGy-B
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample B: 0
Historical
Fabric
3
50 kGy
5/17/2015
WA60-FS-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0
Historical
Fabric
4
50 kGy
5/17/2015
WA60-FS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Fabric
5
50 kGy
5/17/2015
WA60-FS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1s assessment (n=5): 0
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
54

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Check List for the Basic Visual Assessments Historical Metal Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
1st visual assessment after sterilization (performed:5/29/2015, by: Josh Nardin)
Historical
Metal
1
30 kGy
5/18/2015
WA60-MS-GI-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample A: 0
Historical
Metal
2
30 kGy
5/18/2015
WA60-MS-GI-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample B: 0
Historical
Metal
3
30 kGy
5/18/2015
WA60-MS-GI-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0
Historical
Metal
4
30 kGy
5/18/2015
WA60-MS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Metal
5
30 kGy
5/18/2015
WA60-MS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1s assessment (n=5): 0
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
55

-------
Check List for the Basic Visual Assessments Historical Metal Items 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

1
51 visual assessment after sterilization (performed:6/3/2015, by Josh Nardin)


Historical
Metal
1
50 kGy
5/17/2015
WA60-MS-GI-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample A: 0
Historical
Metal
2
50 kGy
5/17/2015
WA60-MS-GI-50kGy-B
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample B: 0
Historical
Metal
3
50 kGy
5/17/2015
WA60-MS-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample C: 0
Historical
Metal
4
50 kGy
5/17/2015
WA60-MS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample D: 0
Historical
Metal
5
50 kGy
5/17/2015
WA60-MS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 1s assessment sample E: 0

Cumulative impact for 1s assessment (n=5): 0
*Grading system/descriptors for 1st visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to the baseline (pre-
exposure) condition
56

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Long-Term Data
Check List for the Basic Visual Assessments Historical Oil Painting 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed:11/5/2015, by: Josh Nardin)
Historical
Oil
Painting
1
30 kGy
5/18/2015
WA60-OPS-GI-30kGy-A
0
0
0
1
0
0
0
Cumulative impact for 2nd assessment sample A:1
Historical
Oil
Painting
2
30 kGy
5/18/2015
WA60-OPS-GI-30kGy-B
0
0
0
1
0
0
0
Cumulative impact for 2nd assessment sample B:1
Historical
Oil
Painting
3
30 kGy
5/18/2015
WA60-OPS-GI-30kGy-C
1
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C:1
Historical
Oil
Painting
4
30 kGy
5/18/2015
WA60-OPS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Oil
Painting
5
30 kGy
5/18/2015
WA60-OPS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 3
Cumulative impact for 1st and 2nd assessment :5.5
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
57

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Check List for the Basic Visual Assessments Historical Oil Painting 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

2
nd visual assessment after sterilization (performed: 11/5/2015, by: Josh Nardin


Historical
Oil
Painting
1
50 kGy
5/17/2015
WA60-OPS-GI-50kGy-A
1
0
0
0.5
0
0
0
Cumulative impact for 2nd assessment sample A: 1.5
Historical
Oil
Painting
2
50 kGy
5/17/2015
WA60-OPS-GI-50kGy-B
1
darker
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B:1
Historical
Oil
Painting
3
50 kGy
5/17/2015
WA60-OPS-GI-50kGy-C
0.5
lighter
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0.5
Historical
Oil
Painting
4
50 kGy
5/17/2015
WA60-OPS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Oil
Painting
5
50 kGy
5/17/2015
WA60-OPS-GI-50kGy-E
0.5
darker
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E:0.5

Cumulative impact for 2nd assessment (n=5): 3.5
Cumulative impact for 1st and 2nd assessment :4
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
58

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Check List for the Basic Visual Assessments Historical Light Hue Monochromatic Pigment Oil
Painting 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed:10/28/2015, by :Josh Nardin)
Light Hue
Monochromatic
Pigment Oil
Painting
1
30 kGy
5/18/2015
WA60-OLT-GI-30kGy-A
0.5
0
0.5
0
0
0
0
Cumulative impact for 2nd assessment sample A:1
Light Hue
Monochromatic
Pigment Oil
Painting
2
30 kGy
5/18/2015
WA60-OLT-GI-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Light Hue
Monochromatic
Pigment Oil
Painting
3
30 kGy
5/18/2015
WA60-OLT-GI-30kGy-C
0.5
0
0.5
0
0
0
0
Cumulative impact for 2nd assessment sample C:1
Light Hue
Monochromatic
Pigment Oil
Painting
4
30 kGy
5/18/2015
WA60-OLT-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Light Hue
Monochromatic
Pigment Oil
Painting
5
30 kGy
5/18/2015
WA60-OLT -G l-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 2
Cumulative impact for 1st and 2nd assessment: 2
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
59

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Check List for the Basic Visual Assessments Historical Light Hue Monochromatic Pigment Oil
Painting 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed: 10/28/2015, by: Josh Nardin)
Light Hue
Monochromatic
Pigment Oil
Painting
1
50 kGy
5/17/2015
WA60-OLT-G l-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Light Hue
Monochromatic
Pigment Oil
Painting
2
50 kGy
5/17/2015
WA60-OLT-G l-50kGy-B
1
darker
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B:1
Light Hue
Monochromatic
Pigment Oil
Painting
3
50 kGy
5/17/2015
WA60-OLT-G l-50kGy-C
0.5
0
0.5
0
0
0
0
Cumulative impact for 2nd assessment sample C:1
Light Hue
Monochromatic
Pigment Oil
Painting
4
50 kGy
5/17/2015
WA60-OLT-G l-50kGy-D
0
0
0.5
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0.5
Light Hue
Monochromatic
Pigment Oil
Painting
5
50 kGy
5/17/2015
WA60-OLT -GI-50kGy-E
0
0
0.5
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0.5

Cumulative impact for 2nd assessment (n=5): 3
Cumulative impact for 1st and 2nd assessment: 3.9
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
60

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Check List for the Basic Visual Assessments Historical Dark Hue Monochromatic Pigment Oil
Painting 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed:11/4/2015, by: Josh Nardin)
Dark Hue
Monochromatic
Pigment Oil
Painting
1
30 kGy
5/18/2015
WA60-ODT-G l-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
2
30 kGy
5/18/2015
WA60-ODT-G l-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
3
30 kGy
5/18/2015
WA60-ODT-G l-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
4
30 kGy
5/18/2015
WA60-ODT-G l-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
5
30 kGy
5/18/2015
WA60-ODT -GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 0
Cumulative impact for 1st and 2nd assessment: 5
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
61

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Check List for the Basic Visual Assessments Historical Dark Hue Monochromatic Pigment Oil
Painting 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed:11/4/2015, by: Josh Nardin)
Dark Hue
Monochromatic
Pigment Oil
Painting
1
50 kGy
5/17/2015
WA60-ODT -G l-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
2
50 kGy
5/17/2015
WA60-ODT -G l-50kGy-B
0
0
0
0
0.5
0
0
Cumulative impact for 2nd assessment sample B: 0.5
Dark Hue
Monochromatic
Pigment Oil
Painting
3
50 kGy
5/17/2015
WA60-ODT -G l-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
4
50 kGy
5/17/2015
WA60-ODT -G l-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Dark Hue
Monochromatic
Pigment Oil
Painting
5
50 kGy
5/17/2015
WA60-ODT -G l-50kGy-E
0
0
0.5
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0.5

Cumulative impact for 2nd assessment (n=5): 1
Cumulative impact for 1st and 2nd assessment :1
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
62

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Check List for the Basic Visual Assessments Historical Documents 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed:11/4/2015, by: Josh Nardin)
Historical
Document
1
30 kGy
5/18/2015
WA60-DS-GI-30kGy-A
1
0
0.5
0
0
0
0
Cumulative impact for 2nd assessment sample A:1.5
Historical
Document
2
30 kGy
5/18/2015
WA60-DS-GI-30kGy-B
1
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B:1
Historical
Document
3
30 kGy
5/18/2015
WA60-DS-GI-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Document
4
30 kGy
5/18/2015
WA60-DS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Document
5
30 kGy
5/18/2015
WA60-DS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 2.5
Cumulative impact for 1s1 and 2nd assessment: 6.6
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
63

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Check List for the Basic Visual Assessments Historical Documents 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors


nd visual assessment after sterilization (performed:11/4/2015, by Josh Nardin


Historical
Document
1
50 kGy
5/17/2015
WA60-DS-GI-50kGy-A
1
lighter
0
0.5 font
faded
0
0
0
0
Cumulative impact for 2nd assessment sample A: 1.5
Historical
Document
2
50 kGy
5/17/2015
WA60-DS-GI-50kGy-B
1
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 1
Historical
Document
3
50 kGy
5/17/2015
WA60-DS-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Document
4
50 kGy
5/17/2015
WA60-DS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Document
5
50 kGy
5/17/2015
WA60-DS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 2.5
Cumulative impact for 1st and 2nd assessment :3.4
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
64

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Check List for the Basic Visual Assessments Historical Books 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

2
ndvisual assessment after sterilization (performed:11/3/2015, by Josh Nardin)


Historical
Book
1
30 kGy
5/18/2015
WA60-BS-GI-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Historical
Book
2
30 kGy
5/18/2015
WA60-BS-GI-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Historical
Book
3
30 kGy
5/18/2015
WA60-BS-GI-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Book
4
30 kGy
5/18/2015
WA60-BS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Book
5
30 kGy
5/18/2015
WA60-BS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 0
Cumulative impact for 1st and 2nd assessment :2.6
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
65

-------
Check List for the Basic Visual Assessments Historical Books 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors


nd visual assessment after sterilization (performed:11/3/2015, by Josh Nardin)


Historical
Book
1
50 kGy
5/17/2015
WA60-BS-GI-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Historical
Book
2
50 kGy
5/17/2015
WA60-BS-GI-50kGy-B
0.5
darker
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0.5
Historical
Book
3
50 kGy
5/17/2015
WA60-BS-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Book
4
50 kGy
5/17/2015
WA60-BS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Book
5
50 kGy
5/17/2015
WA60-BS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 0.5
Cumulative impact for 1st and 2nd assessment: 1.2
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
66

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Check List for the Basic Visual Assessments Historical Photographs 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

2nd
visual assessment after sterilization (performed:11/4/2015, by Josh Nardin)


Historical
Photograph
1
30 kGy
5/18/2015
WA60-PS-GI-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Historical
Photograph
2
30 kGy
5/18/2015
WA60-PS-GI-30kGy-B
0
0
0.5
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0.5
Historical
Photograph
3
30 kGy
5/18/2015
WA60-PS-GI-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Photograph
4
30 kGy
5/18/2015
WA60-PS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Photograph
5
30 kGy
5/18/2015
WA60-PS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 0.5
Cumulative impact for 1st and 2nd assessment :0.5
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
67

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Check List for the Basic Visual Assessments Historical Photographs 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

2nd
visual assessment after sterilization (performed:11/4/2015, by Josh Nardin)


Historical
Photograph
1
50 kGy
5/17/2015
WA60-PS-GI-50kGy-A
0
0
0.5
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0.5
Historical
Photograph
2
50 kGy
5/17/2015
WA60-PS-GI-50kGy-B
0
0
1
lighter
0
0
0
0
Cumulative impact for 2nd assessment sample B:1
Historical
Photograph
3
50 kGy
5/17/2015
WA60-PS-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Photograph
4
50 kGy
5/17/2015
WA60-PS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Photograph
5
50 kGy
5/17/2015
WA60-PS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 1.5
Cumulative impact for 1st and 2nd assessment :1.5
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
68

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Check List for the Basic Visual Assessments Historical Pastel Painting 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed:11/4/2015, by: Josh Nardin)
Historical
Pastel
Painting
1
30 kGy
5/18/2015
WA60-PPS-G l-30kGy-A
0
0
0.5
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0.5
Historical
Pastel
Painting
2
30 kGy
5/18/2015
WA60-PPS-G l-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Historical
Pastel
Painting
3
30 kGy
5/18/2015
WA60-PPS-G l-30kGy-C
1
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 1
Historical
Pastel
Painting
4
30 kGy
5/18/2015
WA60-PPS-G l-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Pastel
Painting
5
30 kGy
5/18/2015
WA60-PPS-G l-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 1.5
Cumulative impact for 1st and 2nd assessment: 6
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
69

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Check List for the Basic Visual Assessments Historical Pastel Painting 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed:11/4/2015, by: Josh Nardin)
Historical
Pastel
Painting
1
50 kGy
5/17/2015
WA60-PPS-GI-50kGy-A
0
0
0.5
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0.5
Historical
Pastel
Painting
2
50 kGy
5/17/2015
WA60-PPS-GI-50kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Historical
Pastel
Painting
3
50 kGy
5/17/2015
WA60-PPS-GI-50kGy-C
0
0
0.5
some
fading
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0.5
Historical
Pastel
Painting
4
50 kGy
5/17/2015
WA60-PPS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Pastel
Painting
5
50 kGy
5/17/2015
WA60-PPS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 1
Cumulative impact for 1st and 2nd assessment: 2
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
70

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Check List for the Basic Visual Assessments Historical Leather Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

2
ld visual assessment after sterilization (performed: 11/2/2015, by Josh Nardin)


Historical
Leather
1
30 kGy
5/18/2015
WA60-LS-GI-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Historical
Leather
2
30 kGy
5/18/2015
WA60-LS-GI-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Historical
Leather
3
30 kGy
5/18/2015
WA60-LS-GI-30kGy-C
0.5
lighter
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0.5
Historical
Leather
4
30 kGy
5/18/2015
WA60-LS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Leather
5
30 kGy
5/18/2015
WA60-LS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 0.5
Cumulative impact for 1st and 2nd assessment: 4.5
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
71

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Check List for the Basic Visual Assessments Historical Leather Items 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors


nd visual assessment after sterilization (performed:11/2/2015, by Josh Nardin


Historical
Leather
1
50 kGy
5/17/2015
WA60-LS-GI-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Historical
Leather
2
50 kGy
5/17/2015
WA60-LS-GI-50kGy-B
0.5
lighter
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0.5
Historical
Leather
3
50 kGy
5/17/2015
WA60-LS-GI-50kGy-C
0.5
lighter
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0.5
Historical
Leather
4
50 kGy
5/17/2015
WA60-LS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Leather
5
50 kGy
5/17/2015
WA60-LS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 1
Cumulative impact for 1st and 2nd assessment: 2.7
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
72

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Check List for the Basic Visual Assessments Historical Wood Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed: 11/2/2015, by: Josh Nardin)
Historical
Wood
1
30 kGy
5/18/2015
WA60-WS-GI-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Historical
Wood
2
30 kGy
5/18/2015
WA60-WS-GI-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Historical
Wood
3
30 kGy
5/18/2015
WA60-WS-GI-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Wood
4
30 kGy
5/18/2015
WA60-WS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Wood
5
30 kGy
5/18/2015
WA60-WS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2ndassessment (n=5): 0
Cumulative impact for 1st and 2nd assessment: 0
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
73

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Check List for the Basic Visual Assessments Historical Wood Items 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed: 11/2/2015, by: Josh Nardin)
Historical
Wood
1
50 kGy
5/17/2015
WA60-WS-GI-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Historical
Wood
2
50 kGy
5/17/2015
WA60-WS-GI-50kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Historical
Wood
3
50 kGy
5/17/2015
WA60-WS-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Wood
4
50 kGy
5/17/2015
WA60-WS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Wood
5
50 kGy
5/17/2015
WA60-WS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 0
Cumulative impact for 1st and 2nd assessment: 1
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
74

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Check List for the Basic Visual Assessments Historical Porcelain Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed:11/3/15, by: Josh Nardin)
Historical
Porcelain
1
30 kGy
5/18/2015
WA60-PBS-GI-30kGy-A
3
3
3
0
0
0
0
Cumulative impact for 2nd assessment sample A:9
Historical
Porcelain
2
30 kGy
5/18/2015
WA60-PBS-GI-30kGy-B
3
3
3
0
0
0
0
Cumulative impact for 2nd assessment sample B:9
Historical
Porcelain
3
30 kGy
5/18/2015
WA60-PBS-GI-30kGy-C
2
2
2
0
0
0
0
Cumulative impact for 2nd assessment sample C:6
Historical
Porcelain
4
30 kGy
5/18/2015
WA60-PBS-GI-30kGy-D
3
3
3
0
0
0
0
Cumulative impact for 2nd assessment sample D:9
Historical
Porcelain
5
30 kGy
5/18/2015
WA60-PBS-GI-30kGy-E
3
3
3
0
0
0
0
Cumulative impact for 2ndassessment sample E:9

Cumulative impact for 2nd assessment (n=5): 42
Cumulative impact for 1st and 2nd assessment :57
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
75

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Check List for the Basic Visual Assessments Historical Porcelain Items 50 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors
2nd visual assessment after sterilization (performed:11/3/2015, by: Josh Nardin)
Historical
Porcelain
1
50 kGy
5/17/2015
WA60-PBS-GI-50kGy-A
2
2
2
0
0
0
0
Cumulative impact for 2nd assessment sample A:6
Historical
Porcelain
2
50 kGy
5/17/2015
WA60-PBS-GI-50kGy-B
3
3
3
0
0
0
0
Cumulative impact for 2nd assessment sample B:9
Historical
Porcelain
3
50 kGy
5/17/2015
WA60-PBS-GI-50kGy-C
3
3
3
0
0
0
0
Cumulative impact for 2nd assessment sample C:9
Historical
Porcelain
4
50 kGy
5/17/2015
WA60-PBS-GI-50kGy-D
2
2
2
0
0
0
0
Cumulative impact for 2nd assessment sample D:6
Historical
Porcelain
5
50 kGy
5/17/2015
WA60-PBS-GI-50kGy-E
3
3
3
0
0
0
0
Cumulative impact for 2nd assessment sample E:9

Cumulative impact for 2nd assessment (n=5): 39
Cumulative impact for 1st and 2nd assessment :61
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
76

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Check List for the Basic Visual Assessments Historical Fabric Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

2
nd visual assessment after sterilization (performed:11/5/2015, by Josh Nardin)


Historical
Fabric
1
30 kGy
5/18/2015
WA60-FS-GI-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Historical
Fabric
2
30 kGy
5/18/2015
WA60-FS-GI-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Historical
Fabric
3
30 kGy
5/18/2015
WA60-FS-GI-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Fabric
4
30 kGy
5/18/2015
WA60-FS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Fabric
5
30 kGy
5/18/2015
WA60-FS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 0
Cumulative impact for 1st and 2nd assessment: 3.5
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
77

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Check List for the Basic Visual Assessments Historical Fabric Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors


nd visual assessment after sterilization (performed:11/5/2015, by Josh Nardin)


Historical
Fabric
1
50 kGy
5/17/2015
WA60-FS-GI-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Historical
Fabric
2
50 kGy
5/17/2015
WA60-FS-GI-50kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Historical
Fabric
3
50 kGy
5/17/2015
WA60-FS-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Fabric
4
50 kGy
5/17/2015
WA60-FS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Fabric
5
50 kGy
5/17/2015
WA60-FS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 0
Cumulative impact for 1st and 2nd assessment: 0
*High level of additional changes for 2nd assessment (3), Moderate level of additional changes for 2nd assessment (2),
Low level of additional changes for 2nd assessment (1), Very low level of additional changes for 2nd assessment (0.5),
No additional changes for 2nd assessment (0)
78

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Check List for the Basic Visual Assessments Historical Metal Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

2
ld visual assessment after sterilization (performed: 11/4/2015, by Josh Nardin)


Historical
Metal
1
30 kGy
5/18/2015
WA60-MS-GI-30kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Historical
Metal
2
30 kGy
5/18/2015
WA60-MS-GI-30kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Historical
Metal
3
30 kGy
5/18/2015
WA60-MS-GI-30kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Metal
4
30 kGy
5/18/2015
WA60-MS-GI-30kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Metal
5
30 kGy
5/18/2015
WA60-MS-GI-30kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 0
Cumulative impact for 1st and 2nd assessment: 0
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
79

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Check List for the Basic Visual Assessments Historical Metal Items 30 kGy
Matrix
Test Date
and
Condition
Sample Code
/Photodocumentation
Code
Noticeable Change in the Physical Characteristics of Material Observed*



Color
Contrast
Fading
Cracking
/Chipping
Brittleness
/Thinning
Legibility
Odors

2
ld visual assessment after sterilization (performed: 11/4/2015, by Josh Nardin)


Historical
Metal
1
50 kGy
5/17/2015
WA60-MS-GI-50kGy-A
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample A: 0
Historical
Metal
2
50 kGy
5/17/2015
WA60-MS-GI-50kGy-B
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample B: 0
Historical
Metal
3
50 kGy
5/17/2015
WA60-MS-GI-50kGy-C
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample C: 0
Historical
Metal
4
50 kGy
5/17/2015
WA60-MS-GI-50kGy-D
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample D: 0
Historical
Metal
5
50 kGy
5/17/2015
WA60-MS-GI-50kGy-E
0
0
0
0
0
0
0
Cumulative impact for 2nd assessment sample E: 0

Cumulative impact for 2nd assessment (n=5): 0
Cumulative impact for 1st and 2nd assessment: 0
*Grading system/descriptors for 2nd visual assessment: 3 -high level of change, 2 -moderate level of change, 1-Low
level of change, 0.5-Very low level of change, 0-No change; relative change as compared to 1st visual assessment
(short term post-exposure) condition
80

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Appendix C:
Short-Term and Long-term Technical Assessment Data
81

-------
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-BS-GI-30kGy-A
1.33
44.53
7.6
14.95
-1.02
0.82
-0.19
1.02 darker
0.19 more saturated
0.82 redder
0.82 redder
0.19 less yellow
0.29
WA60- BS-GI-30kGy-B
1.06
80.61
-0.13
11.68
0.98
-0.24
-0.33
0.98 lighter
0.33 less saturated
0.24 yellower
0.24 less red
0.33 less yellow
0.1
WA60-BS-GI-30kGy-C
0.9
82.58
1.25
16.82
0.45
-0.21
0.75
0.45 lighter
0.73 more saturated
0.27 yellower
0.21 less red
0.75 yellower
0.18
WA60-BS-GI-30kGy-D
1.29
80.72
0.41
13.91
1.13
-0.37
-0.5
1.13 lighter
0.52 less saturated
0.35 yellower
0.37 less red
0.50 less yellow
0.16
WA60-BS-GI-30kGy-E
1
72.47
2.13
18.62
0.9
0.08
-0.42
0.90 lighter
0.41 less saturated
0.13 redder
0.08 redder
0.42 less yellow
0.11
WA60-BS-GI-50kGy-A
1.21
52.46
8.38
21.32
1.01
0
0.66
1.01 lighter
0.62 more saturated
0.24yellower
0.00 redder
0.66 yellower
0.1
WA60-BS-GI-50kGy-B
0.81
80.54
-0.17
11.56
0.56
-0.32
-0.48
0.56 lighter
0.48 less saturated
0.32 yellower
0.32 less red
0.48 less yellow
0.14
WA60-BS-GI-50kGy-C
0.68
82.9
1.28
15.91
0.61
-0.28
-0.11
0.61 lighter
0.13 less saturated
0.27 yellower
0.28 less red
0.11 less yellow
0.09
WA60-BS-GI-50kGy-D
1.05
80.95
0.04
13
0.89
-0.42
-0.36
0.89 lighter
0.37 less saturated
0.41 yellower
0.42 less red
0.36 less yellow
0.14
WA60-BS-GI-50kGy-E
1.28
70.96
-3.03
28.25
-0.09
-1.12
-0.61
0.09 darker
0.51 less saturated
1.17 greener
1.12 greener
0.61 less yellow
0.3
30 and 50 kGy Short-Term Assessment Historical Book Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-DS-GI-30kGy-A
0.77
77.89
-2.32
4.57
0.57
0.19
0.48
0.57 lighter
0.33 more saturated
0.40 yellower
0.19 less green
0.48 yellower
0.15
WA60-DS-GI-30kGy-B
2.06
81.52
0.21
15.91
-0.95
0.11
-1.82
0.95 darker
1.82 less saturated
0.13 redder
0.11 redder
1.82 less yellow
0.34
WA60-DS-GI-30kGy-C
1.31
85.35
-0.62
15.07
1.01
-0.73
-0.39
1.01 lighter
0.38 less saturated
0.74 yellower
0.73 less red
0.39 less yellow
0.23
WA60-DS-GI-30kGy-D
1.1
80.16
0.8
15.93
0.88
-0.39
-0.53
0.88 lighter
0.56 less saturated
0.35 yellower
0.39 less red
0.53 less yellow
0.16
WA60-DS-GI-30kGy-E
1.38
70.5
1.45
14.19
1.19
-0.53
-0.46
1.19 lighter
0.52 less saturated
0.47 yellower
0.53 less red
0.46 less yellow
0.18
WA60-DS-GI-50kGy-A
1.36
78.41
-2.16
5.11
1.05
0.18
0.84
1.05 lighter
0.68 more saturated
0.53 yellower
0.18 less green
0.84yellower
0.25
WA60-DS-GI-50kGy-B
1.24
83.99
0.39
16.76
0.72
-0.05
-1
0.72 lighter
1.00 less saturated
0.03 yellower
0.05 less red
1.00 less yellow
0.18
WA60-DS-GI-50kGy-C
1.24
85.02
-0.63
15.4
0.84
-0.83
-0.37
0.84 lighter
0.36 less saturated
0.84 yellower
0.83 less red
0.37 less yellow
0.26
WA60-DS-GI-50kGy-D
0.47
74.42
3.31
20.3
-0.13
-0.13
0.43
0.13 darker
0.40 more saturated
0.20 yellower
0.13 less red
0.43 yellower
0.12
WA60-DS-GI-50kGy-E
0.8
70.28
1.53
14.1
0.75
-0.26
0.13
0.75 lighter
0.10 more saturated
0.27 yellower
0.26 less red
0.13 yellower
0.1
30 and 50 kGy Short-Term Assessment Historical Document Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-PS-GI-50kGy-A
0.19
72.39
0.77
17.31
0.14
-0.05
-0.12
0.14 lighter
0.12 less saturated
0.04yellower
0.05 less red
0.12 less yellow
0.02
WA60-PS-GI-50kGy-B
1.48
64.4
0.09
6
-0.77
-0.66
-1.08
0.77 darker
1.12 less saturated
0.59 yellower
0.66 less red
1.08 less yellow
0.32
WA60-PS-GI-50kGy-C
1.26
74.21
-0.34
12.06
0.96
-0.31
-0.75
0.96 lighter
0.74 less saturated
0.32 greener
0.31 greener
0.75 less yellow
0.18
WA60-PS-GI-50kGy-D
1.51
50.28
0.23
5.77
1.49
-0.2
0.15
1.49 lighter
0.14 more saturated
0.21 yellower
0.20 less red
0.15 yellower
0.09
WA60-PS-GI-50kGy-E
0.26
45.61
-0.01
6.52
0.06
0.04
-0.25
0.06 lighter
0.25 less saturated
0.04yellower
0.04 less green
0.25 less yellow
0.03
WA60-PS-GI-30kGy-A
0.85
70.6
0.68
17.62
-0.85
-0.03
0.04
0.85 darker
0.04 more saturated
0.03 yellower
0.03 less red
0.04 yellower
0
WA60-PS-GI-30kGy-B
1.06
49.69
-0.25
4.94
-0.75
-0.17
-0.73
0.75 darker
0.73 less saturated
0.19 greener
0.17 greener
0.73 less yellow
0.21
WA60-PS-GI-30kGy-C
1.42
64.21
0.69
14.81
-1.3
-0.39
-0.43
1.30 darker
0.45 less saturated
0.37 yellower
0.39 less red
0.43 less yellow
0.15
WA60-PS-GI-30kGy-D
0.56
46.88
0.26
5.28
0.48
-0.17
0.23
0.48 lighter
0.21 more saturated
0.19 yellower
0.17 less red
0.23 yellower
0.07
WA60-PS-GI-30kGy-E
0.88
57.05
-0.2
9.69
0.6
-0.09
-0.64
0.60 lighter
0.64 less saturated
0.10 greener
0.09 greener
0.64 less yellow
0.13
30 and 50 kGy Short-Term Assessment Historical Photo Surrogate Technical Data
82

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Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-OLT-GI-30kGy-A
1.38
34.39
39.51
14.42
0.96
-0.94
0.3
0.96 lighter
0.78 less saturated
0.60 yellower
0.94 less red
0.30 yellower
0.31
WA60-OLT-GI-30kGy-B
1.41
32.2
35.8
12.37
1.2
-0.35
0.65
1.20 lighter
0.12 less saturated
0.73 yellower
0.35 less red
0.65 yellower
0.22
WA60-OLT-GI-30kGy-C
1.34
33.06
35.99
12.65
1.17
0.03
0.66
1.17 lighter
0.24 more saturated
0.61 yellower
0.03 redder
0.66 yellower
0.22
WA60-OLT-GI-30kGy-D
1.56
33.61
38.46
13.98
0.95
0.45
1.16
0.95 lighter
0.81 more saturated
0.94 yellower
0.45 redder
1.16 yellower
0.12
WA60-OLT-GI-30kGy-E
1.03
33.52
38.16
13.77
0.64
-0.33
0.74
0.64 lighter
0.07 less saturated
0.80 yellower
0.33 less red
0.74 yellower
0.11
WA60-OLT-GI-50kGy-A
3.25
32.61
35.97
12.93
0.79
-3.02
-0.9
0.79 lighter
3.15 less saturated
0.17 yellower
3.02 less red
0.90 less yellow
0.87
WA60-OLT-GI-50kGy-B
3.37
30.36
29.44
9.59
0.63
-3.24
-0.71
0.63 lighter
3.30 less saturated
0.31 yellower
3.24 less red
0.71 less yellow
0.92
WA60-OLT-GI-50kGy-C
3.36
34.11
37.09
13.41
-0.23
-3.2
-1
0.23 darker
3.35 less saturated
0.14 yellower
3.20 less red
1.00 less yellow
0.9
WA60-OLT-GI-50kGy-D
3.14
33.76
35.52
12.83
0.13
-3.06
-0.69
0.13 lighter
3.12 less saturated
0.38 yellower
3.06 less red
0.69 less yellow
0.86
WA60-OLT-GI-50kGy-E
3.11
32.62
35.02
12.32
0.17
-3.04
-0.67
0.17 lighter
3.09 less saturated
0.36 yellower
3.04 less red
0.67 less yellow
0.85
30 and 50 kGy Short-Term Assessment Oil Painting Light Hue Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-ODT-GI-30kGy-A
1.35
30.46
3.17
4.44
1.27
-0.35
-0.28
1.27 lighter
0.44 less saturated
0.12 yellower
0.35 less red
0.28 less yellow
0.14
WA60-ODT-GI-30kGy-B
2.31
30.03
3.15
4.1
2.27
-0.31
0.25
2.27 lighter
0.00 less saturated
0.40 yellower
0.31 less red
0.25 yellower
0.07
WA60-ODT-GI-30kGy-C
1.51
28.45
2.46
3.15
-0.47
-1.08
-0.95
0.47 darker
1.42 less saturated
0.23 yellower
1.08 less red
0.95 less yellow
0.45
WA60-ODT-GI-30kGy-D
1.72
26.92
1.79
1.86
1.24
-1.13
0.38
1.24 lighter
0.69 less saturated
0.97 yellower
1.13 less red
0.38 yellower
0.27
WA60-ODT-GI-30kGy-E
1.06
29.83
3.19
4.21
0.44
-0.78
0.58
0.44 lighter
0.09 less saturated
0.96 yellower
0.78 less red
0.58 yellower
0.19
WA60-ODT-GI-50kGy-A
1.85
27.75
2.12
2.2
1.72
-0.17
0.65
1.72 lighter
0.29 more saturated
0.60 yellower
0.17 less red
0.65 yellower
0.19
WA60-ODT-GI-50kGy-B
1.69
28.17
2.26
2.31
1.64
-0.18
0.36
1.64 lighter
0.10 more saturated
0.39 yellower
0.18 less red
0.36 yellower
0.09
WA60-ODT-GI-50kGy-C
2.03
27.8
2.38
2.99
1.76
0
1.02
1.76 lighter
0.73 more saturated
0.71 yellower
0.00 less red
1.02 yellower
0.29
WA60-ODT-GI-50kGy-D
1.46
29.28
2.81
3.26
1.38
-0.36
0.33
1.38 lighter
0.01 less saturated
0.49 yellower
0.36 less red
0.33 yellower
0.1
WA60-ODT-GI-50kGy-E
0.78
28.04
2.24
3.01
0.08
-0.67
-0.39
0.08 lighter
0.72 less saturated
0.28 yellower
0.67 less red
0.39 less yellow
0.24
30 and 50 kGy Short-Term Assessment Oil painting Dark Hue Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-OPS-GI-30kGy-A
1.41
51.11
-0.75
8.4
0.98
-0.85
0.55
0.98 lighter
0.58 more saturated
0.83 yellower
0.85 less red
0.55 yellower
0.18
WA60-OPS-GI-30kGy-B
0.7
34.35
8.55
11.54
0.64
-0.21
0.18
0.64 lighter
0.02 more saturated
0.28 yellower
0.21 less red
0.18 yellower
0.04
WA60-OPS-GI-30kGy-C
0.69
29.35
1.8
2.01
0.66
0.01
0.2
0.66 lighter
0.15 more saturated
0.13 yellower
0.01 redder
0.20yellower
0.05
WA60-OPS-GI-30kGy-D
0.91
50.07
8.97
30.16
0.85
-0.31
0.15
0.85 lighter
0.05 more saturated
0.34 yellower
0.31 less red
0.15 yellower
0.1
WA60-OPS-GI-30kGy-E
1.19
56.68
-3.54
16.26
-1.19
-0.08
0.11
1.19 darker
0.13 more saturated
0.06 greener
0.08 greener
0.11 yellower
0.11
WA60-OPS-GI-50kGy-A
2.01
48.03
5.57
0.27
0.99
1.08
-1.37
0.99 lighter
0.79 more saturated
1.56 redder
1.08 redder
1.37 less yellow
0.3
WA60-OPS-GI-50kGy-B
3.26
41.87
13.11
25.34
1.97
1.29
2.26
1.97 lighter
2.60 more saturated
0.12 redder
1.29 redder
2.26 yellower
0.4
WA60-OPS-GI-50kGy-C
0.36
26.69
1.93
1.97
-0.22
-0.25
-0.14
0.22 darker
0.28 less saturated
0.07 yellower
0.25 less red
0.14 less yellow
0.09
WA60-OPS-GI-50kGy-D
1.85
50.27
9.01
31.21
1.32
-0.32
1.24
1.32 lighter
1.10 more saturated
0.67 yellower
0.32 less red
1.24 yellower
0.22
WA60-OPS-GI-50kGy-E
1.54
54.22
-0.91
16.03
0.23
0.41
1.47
0.23 lighter
1.43 more saturated
0.52 yellower
0.41 less green
1.47 yellower
0.26
30 and 50 kGy Short-Term Assessment Historical Oil Painting Surrogate Technical Data
83

-------
Data Name
dE*ab(D6B)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-WS-GI-30kGy-A
4.04
27.89
15.23
12.65
3.27
-1.67
1.69
3.27 lighter
0.34 less saturated
2.35 yellower
1.67 less red
1.69 yellower
0.48
WA60-WS-GI-30kGy-B
3.91
25.6
15.6
10.66
-0.69
-1.65
-3.48
0.69 darker
3.41 less saturated
1.79 redder
1.65 less red
3.48 less yellow
0.54
WA60-WS-GI-30kGy-C
2.41
26.14
14.32
10.62
1.71
-1.55
-0.71
1.71 lighter
1.67 less saturated
0.34 yellower
1.55 less red
0.71 less yellow
0.42
WA60-WS-GI-30kGy-D
2.43
27.42
15.67
11.45
1.39
-1.85
-0.73
1.39 lighter
1.93 less saturated
0.48 yellower
1.85 less red
0.73 less yellow
0.51
WA60-WS-GI-30kGy-E
2.94
26.35
14.92
10.91
1.97
-1.7
-1.37
1.97 lighter
2.18 less saturated
0.10 redder
1.70 less red
1.37 less yellow
0.48
WA60-WS-GI-50kGy-A
2.11
26.49
16.87
12.69
1.55
-1.13
-0.87
1.55 lighter
1.42 less saturated
0.02 redder
1.13 less red
0.87 less yellow
0.32
WA60-WS-GI-50kGy-B
3.09
27.63
17.83
13.52
2.37
-1.23
-1.55
2.37 lighter
1.92 less saturated
0.47 redder
1.23 less red
1.55 less yellow
0.35
WA60-WS-GI-50kGy-C
1.57
26.34
16.53
12.22
1.35
-0.77
-0.22
1.35 lighter
0.75 less saturated
0.27yellower
0.77 less red
0.22 less yellow
0.23
WA60-WS-GI-50kGy-D
1.51
27.06
19.22
14.98
0.94
-0.81
-0.85
0.94 lighter
1.16 less saturated
0.17 redder
0.81 less red
0.85 less yellow
0.23
WA60-WS-GI-50kGy-E
2.21
26.26
13.48
9.99
1.34
-1.45
-1
1.34 lighter
1.76 less saturated
0.05 yellower
1.45 less red
1.00 less yellow
0.39
30 and 50 kGy Short-Term Assessment Historical Wood Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness( D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-LS-GI-30kGy-A
1.42
40.09
16
26.36
-0.33
-0.65
-1.21
0.33 darker
1.37 less saturated
0.07 redder
0.65 less red
1.21 less yellow
0.19
WA60-LS-GI-30kGy-B
0.64
40.29
17.03
24.04
-0.51
0.02
-0.37
0.51 darker
0.29 less saturated
0.23 redder
0.02 redder
0.37 less yellow
0.07
WA60-LS-GI-30kGy-C
0.9
41.66
16.02
27.69
-0.65
-0.05
-0.62
0.65 darker
0.56 less saturated
0.27 redder
0.05 less red
0.62 less yellow
0.08
WA60-LS-GI-30kGy-D
1.5
40.21
17.92
25.67
-0.71
-0.33
-1.28
0.71 darker
1.24 less saturated
0.46 redder
0.33 less red
1.28 less yellow
0.14
WA60-LS-GI-30kGy-E
0.88
49.49
12.44
18.47
-0.23
-0.68
-0.5
0.23 darker
0.80 less saturated
0.28 yellower
0.68 less red
0.50 less yellow
0.2
WA60-LS-GI-50kGy-A
1.61
40.74
17.14
25.65
-0.58
-0.78
-1.29
0.58 darker
1.51 less saturated
0.07 redder
0.78 less red
1.29 less yellow
0.24
WA60-LS-GI-50kGy-B
1.84
42.92
15.35
27.61
-0.36
-1.06
-1.46
0.36 darker
1.79 less saturated
0.21 yellower
1.06 less red
1.46 less yellow
0.3
WA60-LS-GI-50kGy-C
0.79
39.61
15.82
25.39
-0.5
-0.23
-0.57
0.50 darker
0.60 less saturated
0.10 redder
0.23 less red
0.57 less yellow
0.09
WA60-LS-GI-50kGy-D
1.51
40.09
17.24
23.99
-0.56
-0.83
-1.12
0.56 darker
1.40 less saturated
0.02 yellower
0.83 less red
1.12 less yellow
0.25
WA60-LS-GI-50kGy-E
0.8
47.97
13.53
20.04
-0.5
-0.56
-0.27
0.50 darker
0.53 less saturated
0.31 yellower
0.56 less red
0.27 less yellow
0.16
30 and 50 kGy Short-Term Assessment Historical Leather Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-MS-GI-30kGy-A
1.13
37.79
1.97
14.58
1.01
0.18
0.46
1.01 lighter
0.48 more saturated
0.12 redder
0.18 redder
0.46 yellower
0.12
WA60-MS-GI-30kGy-B
0.9
34.6
1.71
13.83
0.88
0.2
0.03
0.88 lighter
0.05 more saturated
0.20 redder
0.20 redder
0.03 yellower
0.06
WA60-MS-GI-30kGy-C
1.9
37
2.72
14.81
1.74
0.2
0.74
1.74 lighter
0.77 more saturated
0.06 redder
0.20 redder
0.74 yellower
0.16
WA60-MS-GI-30kGy-D
0.88
36.43
1.93
13.87
0.87
0.12
0
0.87 lighter
0.01 more saturated
0.12 redder
0.12 redder
0.00 less yellow
0.04
WA60-MS-GI-30kGy-E
1.86
38.28
1.98
14.96
1.82
0.13
0.34
1.82 lighter
0.35 more saturated
0.08 redder
0.13 redder
0.34 yellower
0.1
WA60-MS-GI-50kGy-A
1.21
36.71
3.18
14.09
1.18
0.05
0.3
1.18 lighter
0.30 more saturated
0.01 yellower
0.05 redder
0.30 yellower
0.07
WA60-MS-GI-50kGy-B
2.78
36.11
2.13
14.17
2.68
0.2
0.71
2.68 lighter
0.73 more saturated
0.09 redder
0.20 redder
0.71 yellower
0.17
WA60-MS-GI-50kGy-C
1.43
35.99
2.06
14.57
1.29
0.03
0.62
1.29 lighter
0.62 more saturated
0.06 yellower
0.03 redder
0.62 yellower
0.14
WA60-MS-GI-50kGy-D
1.76
35.98
2.17
14.42
1.34
-0.02
1.14
1.34 lighter
1.12 more saturated
0.20yellower
0.02 less red
1.14 yellower
0.24
WA60-MS-GI-50kGy-E
1.66
35.31
2.68
14.48
1.46
0.47
0.63
1.46 lighter
0.70 more saturated
0.36 redder
0.47 redder
0.63 yellower
0.18
30 and 50 kGy Short-Term Assessment Historical Metal Surrogate Technical Data
84

-------
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-PBS-GI-50kGy-A
16
72.96
-3.83
13.63
-11.11
-1.63
11.4
11.11 darker
11.03 more saturated
3.32 yellower
1.63 greener
11.40 yellower
3.39
WA60-PBS-GI-50kGy-B
21.67
68.45
-4.04
16.82
-15.33
-1.83
15.21
15.33 darker
14.56 more saturated
4.75 yellower
1.83 greener
15.21 yellower
4.37
WA60-PBS-GI-50kGy-C
24.82
67.72
-4.04
18.73
-17.52
-1.81
17.49
17.52 darker
16.61 more saturated
5.78 yellower
1.81 greener
17.49 yellower
4.92
WA60-PBS-GI-50kGy-D
17.4
71.16
-4.27
14.81
-11.7
-1.95
12.73
11.70 darker
12.30 more saturated
3.81 yellower
1.95 greener
12.73 yellower
3.71
WA60-PBS-GI-50kGy-E
28.45
65.62
-5.39
22.46
-17.64
-3.11
22.1
17.64 darker
20.79 more saturated
8.12 yellower
3.11 greener
22. lOyellower
6.01
WA60-PBS-GI-30kGy-A
23.89
68.23
-4.57
18.81
-15.43
-2.31
18.09
15.43 darker
16.97 more saturated
6.66 yellower
2.31 greener
18.09 yellower
5.14
WA60-PBS-GI-30kGy-B
22.72
68.04
-4.09
14.01
-18.42
-1.75
13.18
18.42 darker
12.12 more saturated
5.48 yellower
1.75 greener
13.18 yellower
4.03
WA60-PBS-GI-30kGy-C
16.22
68.67
-3.04
9.27
-14.04
-0.74
8.1
14.04 darker
7.17 more saturated
3.83 yellower
0.74 greener
8.10yellower
2.67
WA60-PBS-GI-30kGy-D
22.36
71.12
-5.16
18.88
-13.96
-2.98
17.21
13.96 darker
16.83 more saturated
4.69 yellower
2.98 greener
17.21 yellower
4.88
WA60-PBS-GI-30kGy-E
24.41
69.43
-5.37
20.39
-15.2
-3.18
18.83
15.20 darker
18.40 more saturated
5.13 yellower
3.18 greener
18.83 yellower
5.25
30 and 50 kGy Short-Term Assessment Historical Porcelain Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-FS-GI-30kGy-A
2.92
35.37
26.53
-30.06
1.22
-1.9
1.85
1.22 lighter
2.65 less saturated
0.20 bluer
1.90 less red
1.85 less blue
0.42
WA60-FS-GI-30kGy-B
4.32
35.34
25.35
-29.06
0.17
2.26
-3.67
0.17 lighter
4.25 more saturated
0.75 bluer
2.26 redder
3.67 bluer
1.42
WA60-FS-GI-30kGy-C
0.96
65.93
-17.18
-4.77
0.76
0.59
-0.01
0.76 lighter
0.57 less saturated
0.16 bluer
0.59 less green
0.01 bluer
0.15
WA60-FS-GI-30kGy-D
1.47
67.49
-16.78
-4.14
1.07
0.83
0.55
1.07 lighter
0.94 less saturated
0.33 greener
0.83 less green
0.55 less blue
0.32
WA60-FS-GI-30kGy-E
0.64
80.31
1.87
14.11
-0.09
0.58
-0.26
0.09 darker
0.20 less saturated
0.60 redder
0.58 redder
0.26 less yellow
0.14
WA60-FS-GI-50kGy-A
3.25
34.79
26.74
-30.37
0.3
1.74
-2.73
0.30 lighter
3.20 more saturated
0.53 bluer
1.74 redder
2.73 bluer
1.06
WA60-FS-GI-50kGy-B
2.59
36.74
21.68
-24.35
1.67
-1.21
1.57
1.67 lighter
1.98 less saturated
0.13 redder
1.21 less red
1.57 less blue
0.44
WA60-FS-GI-50kGy-C
0.98
66.71
-16.74
-3.68
0.76
0.55
0.29
0.76 lighter
0.60 less saturated
0.17 greener
0.55 less green
0.29 less blue
0.2
WA60-FS-GI-50kGy-D
1.07
67.81
-17.7
-4.99
0.94
0.48
0.16
0.94 lighter
0.51 less saturated
0.02 greener
0.48 less green
0.16 less blue
0.15
WA60-FS-GI-50kGy-E
0.74
80.26
1.89
14.69
0.4
0.48
0.4
0.40 lighter
0.45 more saturated
0.43 redder
0.48 redder
0.40yellower
0.17
30 and 50 kGy Short-Term Assessment Historical Fabric Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-PPS-GI-30kGy-A
0.8
71.09
-3.37
12.05
0.44
0.61
-0.29
0.44 lighter
0.45 less saturated
0.50 yellower
0.61 less green
0.29 less yellow
0.14
WA60-PPS-GI-30kGy-B
1.84
52.75
9.01
11.34
1.09
-1.44
0.34
1.09 lighter
0.69 less saturated
1.31 yellower
1.44 less red
0.34 yellower
0.36
WA60-PPS-GI-30kGy-C
0.52
76.97
-1.66
11.4
0.46
-0.12
-0.21
0.46 lighter
0.19 less saturated
0.14greener
0.12 greener
0.21 less yellow
0.06
WA60-PPS-GI-30kGy-D
2.19
56.76
0.2
0.71
2.17
-0.21
0.23
2.17 lighter
0.10 more saturated
0.29 yellower
0.21 less red
0.23 yellower
0.05
WA60-PPS-GI-30kGy-E
0.48
77.3
-0.67
13.6
-0.33
-0.02
-0.35
0.33 darker
0.35 less saturated
0.04 greener
0.02 greener
0.35 less yellow
0.04
WA60-PPS-GI-50kGy-A
1.36
71.84
-1.14
13.63
0.59
-0.63
1.05
0.59 lighter
1.08 more saturated
0.57 greener
0.63 greener
1.05 yellower
0.29
WA60-PPS-GI-50kGy-B
1.32
53.21
4.28
9.47
1.27
-0.34
0.14
1.27 lighter
0.02 less saturated
0.36 yellower
0.34 less red
0.14 yellower
0.08
WA60-PPS-GI-50kGy-C
0.47
77.08
-3.39
6.22
-0.46
-0.03
-0.12
0.46 darker
0.09 less saturated
0.09 greener
0.03 greener
0.12 less yellow
0.03
WA60-PPS-GI-50kGy-D
1.23
67.89
-0.2
2.7
1.23
0.09
0.06
1.23 lighter
0.06 more saturated
O.lOyellower
0.09 less green
0.06 yellower
0.03
WA60-PPS-GI-50kGy-E
0.92
78.05
-0.25
11.14
0.81
0.1
-0.42
0.81 lighter
0.43 less saturated
0.09 yellower
0.10 less green
0.42 less yellow
0.08
30 and 50 kGy Short-Term Assessment Historical Pastel Painting Technical Data
85

-------
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-BS-GI-30kGy-A
5.15
46.95
10.98
17.77
1.39
4.21
2.63
1.39 lighter
4.30 more saturated
2.47 redder
4.21 redder
2.63 yellower
1.04
WA60- BS-GI-30kGy-B
0.83
79.99
0.14
12.75
0.36
0.03
0.75
0.36 lighter
0.75 more saturated
0.02 redder
0.03 redder
0.75 yellower
0.22
WA60- BS-GI-30kGy-C
0.63
81.82
1.35
16.61
-0.31
-0.11
0.54
0.31 darker
0.53 more saturated
0.15 yellower
0.11 less red
0.54 yellower
0.11
WA60-BS-GI-30kGy-D
1.19
80.40
1.09
15.22
0.81
0.32
0.81
0.81 lighter
0.83 more saturated
0.26 redder
0.32 redder
0.81 yellower
0.25
WA60- BS-GI-30kGy-E
1.78
71.20
3.79
19.20
-0.37
1.73
0.16
0.37 darker
0.42 more saturated
1.69 redder
1.73 redder
0.16 yellower
0.42
WA60-BS-GI-50kGy-A
1.99
51.66
8.19
22.63
0.22
-0.19
1.97
0.22 lighter
1.77 more saturated
0.88 yellower
0.19 less red
1.97 yellower
0.33
WA60- BS-GI-50kGy-B
0.79
79.93
0.17
12.84
-0.05
0.02
0.79
0.05 darker
0.79 more saturated
0.01 redder
0.02 redder
0.79 yellower
0.22
WA60- BS-GI-50kGy-C
1.19
82.82
1.52
17.09
0.52
-0.04
1.07
0.52 lighter
1.06 more saturated
0.14 yellower
0.04 less red
1.07 yellower
0.22
WA60-BS-GI-50kGy-D
1.57
79.81
0.70
14.89
-0.25
0.25
1.53
0.25 darker
1.54 more saturated
0.19 redder
0.25 redder
1.53 yellower
0.41
WA60- BS-GI-50kGy-E
2.20
70.70
-3.88
29.78
-0.35
-1.97
0.92
0.35 darker
1.11 more saturated
1.87 greener
1.97 greener
0.92 yellower
0.65
30 and 50 kGy Long-Term Assessment Historical Book Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-DS-GI-30kGy-A
1.88
75.68
-2.62
5.00
-1.64
-0.12
0.91
1.64 darker
0.85 more saturated
0.35 yellower
0.12 greener
0.91 yellower
0.24
WA60-DS-GI-30kGy-B
0.89
82.12
0.92
17.70
-0.35
0.82
-0.04
0.35 darker
0.01 less saturated
0.82 redder
0.82 redder
0.04 less yellow
0.17
WA60-DS-GI-30kGy-C
2.25
86.42
0.34
16.29
2.08
0.22
0.83
2.08 lighter
0.83 more saturated
0.21 redder
0.22 redder
0.83 yellower
0.24
WA60-DS-GI-30kGy-D
1.03
78.66
1.34
17.28
-0.62
0.15
0.82
0.62 darker
0.82 more saturated
0.09 redder
0.15 redder
0.82 yellower
0.27
WA60-DS-GI-30kGy-E
1.19
68.19
1.88
15.04
-1.12
-0.10
0.39
1.12 darker
0.37 more saturated
0.14 yellower
0.10 less red
0.39 yellower
0.14
WA60-DS-GI-50kGy-A
2.39
77.40
-2.24
6.66
0.04
0.10
2.38
0.04 lighter
2.15 more saturated
1.04 yellower
0.10 less green
2.38 yellower
0.66
WA60-DS-GI-50kGy-B
1.46
83.94
1.31
18.72
0.68
0.87
0.95
0.68 lighter
0.99 more saturated
0.82 redder
0.87 redder
0.95 yellower
0.34
WA60-DS-GI-50kGy-C
2.41
85.07
0.43
18.00
0.89
0.23
2.23
0.89 lighter
2.23 more saturated
0.19 redder
0.23 redder
2.23 yellower
0.62
WA60-DS-GI-50kGy-D
1.64
73.94
3.87
21.33
-0.61
0.43
1.46
0.61 darker
1.51 more saturated
0.17 redder
0.43 redder
1.46 yellower
0.38
WA60-DS-GI-50kGy-E
0.83
68.86
1.67
14.43
-0.67
-0.12
0.47
0.67 darker
0.45 more saturated
0.18 yellower
0.12 less red
0.47 yellower
0.18
30 and 50 kGy Long-Term Assessment Historical Document Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-PS-GI-50kGy-A
5.42
66.26
0.88
19.15
-5.19
0.18
1.57
5.19 darker
1.58 more saturated
0.11 redder
0.18 redder
1.57 yellower
0.25
WA60-PS-GI-50kGy-B
4.44
46.05
-0.38
5.11
-4.39
-0.30
-0.57
4.39 darker
0.56 less saturated
0.33 greener
0.30 greener
0.57 less yellow
0.21
WA60-PS-GI-50kGy-C
2.96
68.10
0.58
16.58
2.59
-0.51
1.34
2.59 lighter
1.31 more saturated
0.57 yellower
0.51 less red
1.34 yellower
0.37
WA60-PS-GI-50kGy-D
1.53
47.49
0.13
6.08
1.09
-0.30
1.03
1.09 lighter
1.01 more saturated
0.36 yellower
0.30 less red
1.03 yellower
0.27
WA60-PS-GI-50kGy-E
2.65
54.11
-0.53
11.50
-2.34
-0.42
1.16
2.34 darker
1.18 more saturated
0.38 greener
0.42 greener
1.16 yellower
0.25
WA60-PS-GI-30kGy-A
5.65
66.83
0.99
19.03
-5.41
0.17
1.60
5.41 darker
1.60 more saturated
0.09 redder
0.17 redder
1.60 yellower
0.27
WA60-PS-GI-30kGy-B
5.83
59.40
0.16
6.49
-5.77
-0.59
-0.58
5.77 darker
0.62 less saturated
0.55 yellower
0.59 less red
0.58 less yellow
0.25
WA60-PS-GI-30kGy-C
0.84
74.06
-0.20
12.78
0.82
-0.17
-0.03
0.82 lighter
0.03 less saturated
0.17 greener
0.17 greener
0.03 less yellow
0.08
WA60-PS-GI-30kGy-D
1.90
47.17
0.10
6.55
-1.62
-0.33
0.93
1.62 darker
0.92 more saturated
0.37 yellower
0.33 less red
0.93 yellower
0.24
WA60-PS-GI-30kGy-E
5.71
50.41
-0.61
9.73
4.86
-0.56
2.95
4.86 lighter
2.97 more saturated
0.45 greener
0.56 greener
2.95 yellower
0.77
30 and 50 kGy Long-Term Assessment Historical Photograph Surrogate Technical Data
86

-------
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-OLT-GI-30kGy-A
3.65
30.60
42.05
15.79
-2.82
1.61
1.67
2.82 darker
2.08 more saturated
1.02 yellower
1.61 redder
1.67 yellower
0.50
WA60-OLT-GI-30kGy-B
5.17
28.21
39.48
14.51
-2.79
3.34
2.79
2.79 darker
4.07 more saturated
1.55 yellower
3.34 redder
2.79 yellower
1.00
WA60-OLT-GI-30kGy-C
6.01
29.39
40.47
15.07
-2.51
4.52
3.08
2.51 darker
5.28 more saturated
1.40 yellower
4.52 redder
3.08 yellower
1.30
WA60-OLT-GI-30kGy-D
6.59
29.70
42.63
16.47
-2.97
4.63
3.64
2.97 darker
5.59 more saturated
1.84 yellower
4.63 redder
3.64 yellower
1.52
WA60-OLT-GI-30kGy-E
6.02
29.65
42.40
16.26
-3.23
3.92
3.23
3.23 darker
4.78 more saturated
1.70 yellower
3.92 redder
3.23 yellower
1.26
WA60-OLT-GI-50kGy-A
4.08
28.65
41.17
15.20
-3.16
2.17
1.38
3.16 darker
2.51 more saturated
0.56 yellower
2.17 redder
1.38 yellower
0.89
WA60-OLT-GI-50kGy-B
4.05
26.61
34.81
11.76
-3.12
2.13
1.47
3.12 darker
2.48 more saturated
0.73 yellower
2.13 redder
1.47 yellower
0.68
WA60-OLT-GI-50kGy-C
4.03
30.96
42.14
15.60
-3.38
1.85
1.18
3.38 darker
2.14 more saturated
0.48 yellower
1.85 redder
1.18 yellower
0.79
WA60-OLT-GI-50kGy-D
4.52
29.94
40.49
15.31
-3.69
1.91
1.78
3.69 darker
2.41 more saturated
1.02 yellower
1.91 redder
1.78 yellower
0.83
WA60-OLT-GI-50kGy-E
4.35
29.26
40.44
14.76
-3.19
2.38
1.77
3.19 darker
2.84 more saturated
0.87 yellower
2.38 redder
1.77 yellower
0.88
30 and 50 kGy Long-Term Assessment Oil Painting Light Hue Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-ODT-GI-30kGy-A
3.19
26.01
3.20
4.89
-3.17
-0.32
0.17
3.17 darker
0.05 less saturated
0.36 yellower
0.32 less red
0.17 yellower
0.12
WA60-ODT-GI-30kGy-B
2.22
25.75
3.26
4.77
-2.00
-0.19
0.93
2.00 darker
0.61 more saturated
0.73 yellower
0.19 less red
0.93 yellower
0.18
WA60-ODT-GI-30kGy-C
4.12
24.90
2.74
4.50
-4.02
-0.79
0.41
4.02 darker
0.14 less saturated
0.88 yellower
0.79 less red
0.41 yellower
0.23
WA60-ODT-GI-30kGy-D
4.01
21.98
1.53
2.10
-3.70
-1.39
0.63
3.70 darker
0.67 less saturated
1.37 yellower
1.39 less red
0.63 yellower
0.38
WA60-ODT-GI-30kGy-E
4.06
25.58
3.29
4.85
-3.81
-0.67
1.22
3.81 darker
0.49 more saturated
1.30 yellower
0.67 less red
1.22 yellower
0.28
WA60-ODT-GI-50kGy-A
3.24
22.89
1.90
2.23
-3.15
-0.39
0.68
3.15 darker
0.16 more saturated
0.77 yellower
0.39 less red
0.68 yellower
0.19
WA60-ODT-GI-50kGy-B
3.07
23.53
1.99
2.40
-3.00
-0.46
0.45
3.00 darker
0.01 less saturated
0.64 yellower
0.46 less red
0.45 yellower
0.16
WA60-ODT-GI-50kGy-C
3.43
22.76
2.00
2.90
-3.28
-0.39
0.92
3.28 darker
0.42 more saturated
0.91 yellower
0.39 less red
0.92 yellower
0.22
WA60-ODT-GI-50kGy-D
2.68
25.48
2.91
4.04
-2.42
-0.26
1.11
2.42 darker
0.66 more saturated
0.93 yellower
0.26 less red
1.11 yellower
0.25
WA60-ODT-GI-50kGy-E
3.25
24.89
2.67
4.46
-3.06
-0.24
1.06
3.06 darker
0.72 more saturated
0.81 yellower
0.24 less red
1.06 yellower
0.22
30 and 50 kGy Long-Term Assessment Oil Painting Dark Hue Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-OPS-GI-30kGy-A
4.24
46.54
-1.84
9.03
-3.59
-1.94
1.18
3.59 darker
1.36 more saturated
1.81 yellower
1.94 less red
1.18 yellower
0.42
WA60-OPS-GI-30kGy-B
8.05
33.80
11.33
18.99
0.10
2.57
7.63
0.10 lighter
7.77 more saturated
2.12 yellower
2.57 redder
7.63 yellower
1.02
WA60-OPS-GI-30kGy-C
4.49
24.21
1.56
2.00
-4.48
-0.23
0.18
4.48 darker
0.02 less saturated
0.30 yellower
0.23 less red
0.18 yellower
0.09
WA60-OPS-GI-30kGy-D
3.69
46.91
9.82
32.84
-2.31
0.54
2.82
2.31 darker
2.86 more saturated
0.30 yellower
0.54 redder
2.82 yellower
0.15
WA60-0 PS-G1- 30kGy- E
2.44
55.69
-3.87
17.17
-2.17
-0.41
1.02
2.17 darker
1.09 more saturated
0.19 greener
0.41 greener
1.02 yellower
0.31
WA60-OPS-GI-50kGy-A
2.26
45.87
6.31
0.97
-1.17
1.81
-0.68
1.17 darker
1.59 more saturated
1.10 redder
1.81 redder
0.68 less yellow
0.37
WA60-OPS-GI-50kGy-B
6.94
39.45
13.09
29.89
-0.45
1.27
6.81
0.45 darker
6.70 more saturated
1.76 yellower
1.27 redder
6.81 yellower
0.42
WA60-OPS-GI-50kGy-C
3.89
23.30
2.91
3.37
-3.61
0.72
1.25
3.61 darker
1.41 more saturated
0.33 yellower
0.72 redder
1.25 yellower
0.34
WA60-OPS-GI-50kGy-D
3.92
46.99
9.55
33.35
-1.96
0.21
3.39
1.96 darker
3.31 more saturated
0.77 yellower
0.21 redder
3.39 yellower
0.27
WA60-OPS-GI-50kGy-E
2.92
52.59
-1.18
17.12
-1.40
0.14
2.55
1.40 darker
2.53 more saturated
0.34yellower
0.14 less green
2.55 yellower
0.41
30 and 50 kGy Long-Term Assessment Historical Oil Painting Surrogate Technical Data
87

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Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-WS-GI-30kGy-A
7.15
23.64
19.50
17.54
-0.98
2.59
6.58
0.98 darker
6.08 more saturated
3.62 yellower
2.59 redder
6.58 yellower
0.74
WA60-WS-GI-30kGy-B
6.70
20.52
20.20
15.84
-5.78
2.95
1.70
5.78 darker
3.36 more saturated
0.52 redder
2.95 redder
1.70 yellower
0.77
WA60-WS-GI-30kGy-C
5.39
21.38
18.29
15.05
-3.06
2.42
3.72
3.06 darker
4.19 more saturated
1.47 yellower
2.42 redder
3.72 yellower
0.62
WA60-WS-GI-30kGy-D
4.86
23.53
19.73
15.71
-2.51
2.21
3.53
2.51 darker
3.88 more saturated
1.51 yellower
2.21 redder
3.53 yellower
0.58
WA60-WS-GI-30kGy-E
4.42
21.97
18.65
15.37
-2.41
2.02
3.09
2.41 darker
3.50 more saturated
1.19 yellower
2.02 redder
3.09 yellower
0.51
WA60-WS-GI-50kGy-A
6.33
21.78
21.18
18.02
-3.16
3.19
4.46
3.16 darker
5.28 more saturated
1.48 yellower
3.19 redder
4.46 yellower
0.81
WA60-WS-GI-50kGy-B
4.29
23.33
21.18
18.25
-1.93
2.13
3.18
1.93 darker
3.67 more saturated
1.09 yellower
2.13 redder
3.18 yellower
0.53
WA60-WS-GI-50kGy-C
6.14
21.39
19.81
16.73
-3.61
2.51
4.28
3.61 darker
4.62 more saturated
1.83 yellower
2.51 redder
4.28 yellower
0.64
WA60-WS-GI-50kGy-D
6.78
22.40
22.69
20.84
-3.72
2.67
5.01
3.72 darker
5.28 more saturated
2.07 yellower
2.67 redder
5.01 yellower
0.70
WA60-WS-GI-50kGy-E
5.51
21.10
16.82
14.48
-3.82
1.89
3.49
3.82 darker
3.65 more saturated
1.54yellower
1.89 redder
3.49 yellower
0.48
30 and 50 kGy Long-Term Assessment Historical Wood Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-LS-GI-30kGy-A
4.64
38.04
17.51
31.45
-2.39
0.86
3.88
2.39 darker
3.79 more saturated
1.20 yellower
0.86 redder
3.88 yellower
0.18
WA60-LS-GI-30kGy-B
5.06
37.46
18.34
27.97
-3.35
1.33
3.56
3.35 darker
3.69 more saturated
0.89 yellower
1.33 redder
3.56 yellower
0.31
WA60-LS-GI-30kGy-C
4.98
39.43
17.17
32.22
-2.88
1.10
3.91
2.88 darker
3.96 more saturated
0.92 yellower
1.10 redder
3.91 yellower
0.20
WA60-LS-GI-30kGy-D
4.76
37.57
19.55
30.08
-3.34
1.30
3.13
3.34 darker
3.33 more saturated
0.64 yellower
1.30 redder
3.13 yellower
0.28
WA60-LS-GI-30kGy-E
3.05
47.15
13.28
20.60
-2.58
0.17
1.62
2.58 darker
1.44 more saturated
0.76 yellower
0.17 redder
1.62 yellower
0.12
WA60-LS-GI-50kGy-A
4.72
37.71
18.70
29.88
-3.60
0.77
2.95
3.60 darker
2.89 more saturated
0.95 yellower
0.77 redder
2.95 yellower
0.16
WA60-LS-GI-50kGy-B
3.81
40.37
16.73
31.50
-2.91
0.32
2.43
2.91 darker
2.28 more saturated
0.88 yellower
0.32 redder
2.43 yellower
0.12
WA60-LS-GI-50kGy-C
4.78
37.45
17.06
29.79
-2.66
1.01
3.84
2.66 darker
3.82 more saturated
1.09 yellower
1.01 redder
3.84yellower
0.21
WA60-LS-GI-50kGy-D
4.34
37.70
18.73
28.23
-2.95
0.66
3.12
2.95 darker
2.94 more saturated
1.24 yellower
0.66 redder
3.12 yellower
0.15
WA60-LS-GI-50kGy-E
2.76
46.21
14.25
21.87
-2.27
0.17
1.57
2.27 darker
1.39 more saturated
0.74 yellower
0.17 redder
1.57 yellower
0.09
30 and 50 kGy Long-Term Assessment Historical Leather Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-MS-GI-30kGy-A
3.60
35.60
1.90
17.52
-1.17
0.11
3.40
1.17 darker
3.39 more saturated
0.29 yellower
0.11 redder
3.40 yellower
0.52
WA60-MS-GI-30kGy-B
4.10
31.54
1.67
17.27
-2.18
0.16
3.47
2.18 darker
3.47 more saturated
0.19 yellower
0.16 redder
3.47 yellower
0.48
WA60-MS-GI-30kGy-C
3.65
33.93
2.87
17.45
-1.33
0.35
3.38
1.33 darker
3.39 more saturated
0.23 yellower
0.35 redder
3.38 yellower
0.48
WA60-MS-GI-30kGy-D
3.13
35.07
2.27
16.93
-0.49
0.47
3.05
0.49 darker
3.09 more saturated
0.06 redder
0.47 redder
3.05 yellower
0.45
WA60-MS-GI-30kGy-E
3.53
35.20
1.79
17.92
-1.26
-0.06
3.30
1.26 darker
3.27 more saturated
0.43 yellower
0.06 less red
3.30 yellower
0.52
WA60-MS-GI-50kGy-A
3.38
33.47
2.97
16.45
-2.07
-0.16
2.66
2.07 darker
2.58 more saturated
0.69 yellower
0.16 less red
2.66 yellower
0.39
WA60-MS-GI-50kGy-B
3.90
30.13
1.98
15.56
-3.29
0.05
2.10
3.29 darker
2.09 more saturated
0.23 yellower
0.05 redder
2.10 yellower
0.30
WA60-MS-GI-50kGy-C
3.97
32.80
2.00
17.43
-1.90
-0.03
3.48
1.90 darker
3.45 more saturated
0.47 yellower
0.03 less red
3.48 yellower
0.52
WA60-MS-GI-50kGy-D
4.39
32.61
1.87
17.15
-2.03
-0.32
3.88
2.03 darker
3.80 more saturated
0.84yellower
0.32 less red
3.88 yellower
0.62
WA60-MS-GI-50kGy-E
4.14
31.38
2.06
17.17
-2.48
-0.15
3.32
2.48 darker
3.27 more saturated
0.60yellower
0.15 less red
3.32 yellower
0.50
30 and 50 kGy Long-Term Assessment Historical Metal Surrogate Technical Data
88

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Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-PBS-GI-50kGy-A
20.70
69.95
-5.27
15.93
-13.71
-3.00
15.21
13.71 darker
14.40 more saturated
5.75 yellower
3.00 greener
15.21 yellower
4.58
WA60-PBS-GI-50kGy-B
20.26
69.81
-4.65
12.15
-16.65
-2.31
11.31
16.65 darker
10.53 more saturated
4.75 yellower
2.31 greener
11.31 yellower
3.58
WA60-PBS-GI-50kGy-C
15.46
69.11
-3.53
8.43
-13.60
-1.22
7.25
13.60 darker
6.55 more saturated
3.34 yellower
1.22 greener
7.25 yellower
2.41
WA60-PBS-GI-50kGy-D
18.69
73.28
-5.55
15.77
-11.80
-3.37
14.11
11.80 darker
13.98 more saturated
3.88 yellower
3.37 greener
14.11 yellower
4.25
WA60-PBS-GI-50kGy-E
20.78
70.64
-5.73
16.50
-13.99
-3.54
14.95
13.99 darker
14.79 more saturated
4.18 yellower
3.54 greener
14.95 yellower
4.48
WA60-PBS-GI-30kGy-A
12.76
74.70
-4.16
10.67
-9.37
-1.96
8.44
9.37 darker
8.32 more saturated
2.42 yellower
1.96 greener
8.44 yellower
2.68
WA60-PBS-GI-30kGy-B
18.35
70.36
-4.56
13.91
-13.42
-2.35
12.30
13.42 darker
11.90 more saturated
3.89 yellower
2.35 greener
12.30yellower
3.74
WA60-PBS-GI-30kGy-C
23.34
68.05
-4.81
16.82
-17.19
-2.58
15.58
17.19 darker
14.94 more saturated
5.11 yellower
2.58 greener
15.58 yellower
4.57
WA60-PBS-GI-30kGy-D
14.45
72.37
-4.55
11.77
-10.48
-2.23
9.69
10.48 darker
9.51 more saturated
2.92 yellower
2.23 greener
9.69 yellower
2.98
WA60-PBS-GI-30kGy-E
24.78
67.52
-6.11
19.11
-15.74
-3.83
18.75
15.74 darker
17.75 more saturated
7.14 yellower
3.83 greener
18.75 yellower
5.44
30 and 50 kGy Long-Term Assessment Historical Porcelain Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-FS-GI-30kGy-A
4.12
32.62
26.72
-28.50
-1.53
-1.72
3.41
1.53 darker
3.68 less saturated
1.03 redder
1.72 less red
3.41 less blue
2.08
WA60-FS-GI-30kGy-B
9.59
32.97
29.24
-32.41
-2.20
6.16
-7.02
2.20 darker
9.34 more saturated
0.15 bluer
6.16 redder
7.02 bluer
1.52
WA60-FS-GI-30kGy-C
2.10
66.75
-19.13
-4.55
1.58
-1.37
0.21
1.58 lighter
1.27 more saturated
0.54 greener
1.37 greener
0.21 less blue
0.36
WA60-FS-GI-30kGy-D
2.41
68.40
-18.56
-3.72
1.98
-0.95
0.98
1.98 lighter
0.71 more saturated
1.17 greener
0.95 greener
0.98 less blue
0.51
WA60-FS-GI-30kGy-E
3.01
82.40
2.55
16.23
2.01
1.25
1.85
2.01 lighter
1.99 more saturated
1.02 redder
1.25 redder
1.85 yellower
0.61
WA60-FS-GI-50kGy-A
3.44
33.38
27.49
-29.74
-1.10
2.48
-2.10
1.10 darker
3.23 more saturated
0.41 redder
2.48 redder
2.10 bluer
0.36
WA60-FS-GI-50kGy-B
2.03
34.75
24.65
-26.88
-0.32
1.76
-0.96
0.32 darker
1.89 more saturated
0.66 redder
1.76 redder
0.96 bluer
0.83
WA60-FS-GI-50kGy-C
2.23
67.37
-18.94
-3.50
1.43
-1.65
0.47
1.43 lighter
1.52 more saturated
0.80 greener
1.65 greener
0.47 less blue
0.46
WA60-FS-GI-50kGy-D
1.71
67.77
-19.11
-4.03
0.89
-0.93
1.13
0.89 lighter
0.63 more saturated
1.31 greener
0.93 greener
1.13 less blue
0.56
WA60-FS-GI-50kGy-E
4.06
83.04
2.67
16.48
3.18
1.26
2.18
3.18 lighter
2.33 more saturated
0.96 redder
1.26 redder
2.18 yellower
0.67
30 and 50 kGy Long-Term Assessment Historical Fabric Surrogate Technical Data
Data Name
dE*ab(D65)
L*(D65)
a*(D65)
b*(D65)
dL*(D65)
da*(D65)
db*(D65)
Lightness(D65)
Saturation(D65)
Hue(D65)
a* Evaluation(D65)
b* Evaluation(D65)
MI(D65, A)
WA60-PPS-GI-30kGy-A
1.30
70.47
-3.89
11.05
-0.17
0.08
-1.28
0.17 darker
1.24 less saturated
0.34 greener
0.08 less green
1.28 less yellow
0.30
WA60-PPS-GI-30kGy-B
5.12
47.36
7.80
10.09
-4.29
-2.64
-0.91
4.29 darker
2.42 less saturated
1.41 yellower
2.64 less red
0.91 less yellow
0.73
WA60-PPS-GI-30kGy-C
1.46
75.34
-2.42
11.60
-1.16
-0.88
-0.01
1.16 darker
0.14 more saturated
0.87 greener
0.88 greener
0.01 less yellow
0.24
WA60-PPS-GI-30kGy-D
3.17
57.65
0.06
1.23
3.06
-0.36
0.75
3.06 lighter
0.59 more saturated
0.58 yellower
0.36 less red
0.75 yellower
0.21
WA60-PPS-GI-30kGy-E
2.37
75.30
-0.71
14.40
-2.33
-0.06
0.45
2.33 darker
0.45 more saturated
0.04 greener
0.06 greener
0.45 yellower
0.11
WA60-PPS-GI-50kGy-A
1.56
72.15
-1.51
13.38
0.91
-1.00
0.79
0.91 lighter
0.87 more saturated
0.93 greener
1.00 greener
0.79 yellower
0.32
WA60-PPS-GI-50kGy-B
4.90
54.32
7.15
12.77
2.38
2.54
3.45
2.38 lighter
4.23 more saturated
0.63 redder
2.54 redder
3.45 yellower
0.99
WA60-PPS-GI-50kGy-C
0.30
77.30
-3.37
6.53
-0.24
0.00
0.19
0.24 darker
0.17 more saturated
0.08 yellower
0.00 greener
0.19 yellower
0.06
WA60-PPS-GI-50kGy-D
5.28
61.46
-0.52
1.81
-5.21
-0.23
-0.82
5.21 darker
0.76 less saturated
0.38 greener
0.23 greener
0.82 less yellow
0.20
WA60-PPS-GI-50kGy-E
3.26
74.11
-0.20
12.48
-3.13
0.15
0.92
3.13 darker
0.91 more saturated
0.17 yellower
0.15 less green
0.92 yellower
0.24
30 and 50 kGy Long-Term Assessment Historical Pastel Painting Surrogate Technical Data
89

-------
Appendix D: Certificates for Irradiation
II
i
90

-------
terigenics
Last Major Irradiator Qualification: 07/18/2014
Dose Distribution Map Certification
Date (mm/dd/yyyy)
Processing Flow:
WO#: 1511838
A5
LmiIIm 41* Tnunm Alt*;
Page
1 of 1
DoseWlap Id:
Pkg Per Tote:
116_M14_0488
1
Irradiation Date:14 MAY 15 13:04:00
Location:
Tote 1
0A4
18.4
0C4
16.7
0C5
16.7
0C9
16.8
6A5
19.3
7A5
19.6
TA4
21.5
Sterigenics certifies that the dosages listed above are representative readings of each
position and accuracy of the dosimetry system utilized. Based on the statistical
equivalency of the values, the high and low locations to be monitored on future runs will be
as follows:
High: TA4	Low: 0C4
Certified By:	Date: 5/18/15 10:22	
Verified BN^jjrp0 BJDate: \ctU iCUiiVO
Date/Time Format: MM/DD/YYXX.XX
Document N°: HA-F-019	Revision N°: 1
User must verify the revision number of printed or downloaded document against the effectiveversion. Confidential Information
Page 1 of 1
91

-------
A
ww
TOPS
HAW RIVER PALLET DEFAULT
Date Printed : 5/13/2015
Last Saved : 5/12/2015 !
GMA (Notched) 48x40x5
Package
(OP)
16.000 in
16.000 in
48.750 in
12.000 lb
Cubic Efficiency:
Cases per layer:
Layers/load:
Dns (g/cc): 0.0266
Ln
Wd
Ht
Wt
UnitLoad
(Pallet Inc)
48 in
40 in
54 in
77 lb
1
10.00%
1
1
0.0266
54
(OD)
16.000 ^ 16.000
(OD)	(OD)
Customer Name: ARCADIS
Dose Map #: M14-0488
Cell(s): A
Flow: A5
Notes: 3-PALLET RISE HEIGHT PRODUCT FILL 2n FROM TOP OF CTN. 3.50" IN FROM THE SIDE USE TAPEICLSTABIL1ZE LOAD.
Sterigenics - 03:36 pm (TOPS Pro Version 6.02)
m__. I 0 CC
R5S30460
RIS0003
As of Date:	05/18/15
Customer Name:	Arcadia U.S., Inc.- Highlands Ranch, CO.
Customer Number 244116
Sterigenics
Bill of Material Print
Long Address Number P-B-S-USARCA01
Date- 05/18/15
Time- 12:52:50 GMT
Page -	1 of 1
116 M14 0489
Parent Description
Requested UOM
Description Line 2
H1TA4 LO: 0C4
Component Description
ARC_DOC_SAMPLES_50 DOC SAMPLES 50
Description Line 2
Document Samples 50 kGy
Seq No
Dimensions H - W - D Density Weight
Product Code: General Industrial Other
92

-------
terigemcs
Last Major Irradiator Qualification:
Dose Distribution Map Certification
07/18/2014	Page 1 of 1
Processing Flow:
WO#: 1511840
A5
Date (mm/dd/yyyy)
HlWH <>• rrMtfi'H aiit*
REAR (B)
MIDDLE (C) TrM
• ill
FRONT (A)
DoseMap Id:
Pkg Per Tote:
116_M14_0489
1
Irradiation Date:14 MAY 15 14:02:00
Location:
Tote 1
0A4
26.9
0C4
24.0
0C5
24.4
0C9
24.2
6A5
28.2
7A5
28.6
TA4
31.0
Sterigenics certifies that the dosages listed above are representative readings of each
position and accuracy of the dosimetry system utilized. Based on the statistical
equivalency of the values, the high and low locations to be monitored on future runs will be
as follows:
High: TA4	Low: 0C4
Certified By: Vji A*t,rl	 Date: 5/18/15 8:39	
Verified By:	C ipA 0 1 k <") " Date:	~
Date/Time Format: MM/DD/YY XX.XX
Document N°: HA-F-019	Revision N°:	1
User must verify the revision number of printed or downloaded document against the effectiveversion. Confidential Information
Page 1 of 1
93

-------
WA7
TOPS
HAW RIVER
HAW RIVER PALLET DEFAULT
Date Printed : 5/13/2015
Last Saved : 5/12/2015
GMA (Notched) 48x40x5
Package
(OD)
54
16.000 in
16.000 in
48.750 in
12.000 lb
Ln:
Wd:
Ht:
Wt:
Package:
Cubic Efficiency:
Cases per layer:
Layers/load:
Dns (g/cc):0.0266
UnitLoad
(Pallet Inc)
48 in
40 in
54 in
77 lb
1
10.00%
1
1
0.0266
(OD)
16.000 ^ 16.000
(OD)	(OD)
Customer Name: ARCADIS
Dose Map#: M14-0489
Cell(s): A
Flow: A5
Notes: 3-PALLET RISE HEIGHT PRODUCT FILL 2" FROM TOP OF CTN. 3.50" IN FROM THE SIDE USE TAPI
Sterig«nics - 03:38 pm (TOPS Pro Version 6.02)
STABILIZE LOAD

	J
Eric Hesle?
94

-------
/,
y Sterigenics.
Certificate of Processing
STERIGENICS 1148 Porter Ave. Haw River NC 27258
TEL 336 S78-5876 FAX 336 578-5859 www.sterigenics.com
05/18/2015 12:57:05 GMT
Page 1 of 1
Customer Name;	Arcadis U.S., Inc.- Highlands Ranch. CO. Processing Facility:
P,0.#	D15-04S
50.0-No Max	ARC, Gamma Treatment
Received Date/Time:
Work Order #
Sales Order
1511840
1317006
05/13/2015 14:12:00 GMT
SO
Line#
Qty UOM Customer Item Number
Customer item Description
Customer
Lot Number
Customer
Load Number
1 EA
1 EA
DOC SAMPLES 50
Total
Document Samples 50 kGy
NA
Quality Test Summary
Op# Quality Test Description
Minimum Spec Maximum Spec Result Pass/Fail User
--Signed By-
3/Time
450.00 Minimum Dose
50.0 kGy	300,0 kGy
Reason Code Test
53.3 KGY Pass
BBUNTIN
BRAD BUNTIN
05/17/2015 11:04:15 GMT
Sterigenics certifies that the materials fisted above (as described by the Manufacturer) received the indicated doses within the precision and accuracy of the
dosimetry system employed.
Electronically Signed By: STEFANI ALLRED
Reason: Work Order Completions
Date: 05/18/2015 12:56:09 GMT
/
IP
R55480102
Sterigenics.
Certificate of Processing
STERIGENICS 1148 Porter Ave. Haw River NC 27258
TEL 338 578-5876 FAX 336 578-5859 www.sterigemcs.com
05/19/2015 13:33:05 GMT
Page 1 of 1
Customer Name:
P.O.#
Arcadis U.S., Inc.- Highlands Ranch, CO.
D15-Q45
Processing Facility:
ARC, Gamma Treatment
Received Date/Time:
Work Order #
Sales Order#
1511838
1317003
05/13/2015 14:12:00 GMT
SO
Line#
Qty UOM Customer Item Number
Customer Item Description
Customer
Lot Number
Customer
Load Number
EA DOC SAMPLES
EA Total
Document Sample 30 kGy
Quality Test Summary
Op# Quality Test Description
Minimum Spec Maximum Spec Result Pass/Fail User
- -Signed By -
450.00 Minimum Dose
30.0 kGy	300,0 kGy
Reason Code Test
33,3 KGY Pass
THARVEY
Timothy Harvey
05/18/2015 09:52:45 GMT
Sterigenics certifies that the materials listed above {as described by the Manufacturer) received the indicated doses within the precision and accuracy of the
dosimetry system employed.
Electronically Signed By; STEFANI ALLRED
Reason: Work Order Completions
Date: 05/18/201514:31:19GMT
95

-------
96

-------
Appendix E: Sterilization Verification Data
ARCAD1S
*915 Frtsnsctus litvi
Durham. NC 27713
[919)511-3662
FAX (9191511-5690
Page 1
Report lo:
Barbara Wyr/yktivvska-
Ccrailini
Bill lo;
Not Applicable
Chain of Custody Record
I'ROJECT NUMBER
RNW0Z76.O06O
PCI#
Not Applicable
u
a
75
c
&
"mm
O
c
7L
Analysis
Required
LABORATORY;
Onsite Microbiology Laboratory c/o Nicole Griffin Gatchalian
PROJECT N AM1'. Material Compatibility of Gamma Irradiation
t.Afl ADDRESS
EPA/RTF M
Kll l H I i fl IIV (XW\ITI'SK)
Hi)rharn Wyr/y k rm ska-Cc ra di n i
REPORT FORM VI {CIRCLE ALL REQUIRED)
El .EC T KOMI A ERflAt. I AX/IIARIX Of\
FIELD
SAMPLE II)
RUSH
FACTOR
SAMPLE
Matrix
DATE/
TIME
REMARKS
LAB ID NO.
(for tab uw out})
/WA 6-tfG ISl-ttMtrophuvux DS-30 kGy
t week
TAT
Bl lest strip
A I CC W 9372
5/26/14
1
Qua Illative
Documents test set Bl 1,
irradiated at 30kGy
••ft'1 A Mil) m-B airophaais WS-30 kGy
1 week
TAT
Bl tesi strip
ATCC X 9372
5/26/14
1
Qualitative
Wood test set Bl I,
irradiated at 30 kGy

WA 6-60 B1 - /i- ttiraphueus ODT-30 kCiv
1 week
TAT
Bl lest strip
ATCC H 9372
5/26' 14
1
Qualitative
Oil dark surrogate test set
Bl I. irradiated at 30 kGv

^WA 6-60 B1 -/?.airopluictts OLT-30 kGy
1 week
TAT
Bl lest strip
ATCC ti 9372
5/26/14
1
Qualitative
Oil tight surrogate test set
Bl 1. Irradiated at 30 kGv
"WA 6-60 Bl-fl. utrnphovus FS-30 kGv
1 week
TAT
Bl lest strip
ATCC * 9372
5/26 T 4
1
Qualitative
Fabric tesi set Bll.
Irradiated at 30 kGv
"'WA 6-60 \i\-tf.titr<>pluH'Us MS-30 kGy
1 week
TAT
Bl lest strip
ATCC « 9372
5/26/14
1
Qualitative
Metal test set Bl 1.
Irradiated at 30 kGy
WA 6-60 Bl-B.altvphat'us PBS-30 kGy
1 week
TAT
Bl lest strip
ATCC # 9372
5/26/14
I
Qualitative
Porcelain test set Bll.
Irradiated at 30 kGy

WA 6-60 \\\-[Latrophsii-m BS-30 kGy
1 week
TAT
Bl test strip
A I CC W 9372
5/26/14
1
Qualitative
Books lest sic Bl 1,
Irradiated at 30 kGv

WA 6-60 Bt-fi.afmptujcus PS-30 kGy
1 week
TAT
Bt test strip
ATCC if 9372
5/26/14
1
Qualitative
Photographs test set Bl 1.
Irradiated at 30 kGy

WA 6-60 B1-J3.atrophacwt OPS-30 kGy
1 week
TAT
Bl tesi strip
ATCC « 9372
5/26/14
1
Qualitative
Oil historical test set Bll.
Irradiated at 30 kGy

WA 6-60 HI-B.atraptmem I S-30 kGy
1 week
TAT
Bl tesi strip
ATCC M 9372
5/26/14
1
Qualitative
Leather test set Bll.
Irradiated at 30 kGv
WA 6-61) Bl-fi.atraphtNm l'PS-30 kGy
1 week
TAT
Bl tesi strip
ATCC it 9372
5/26/14
1
Qualitative
Paslel test set Bll.
Irradiated at 30 kGy



97

-------
ARCADIS
6-60 Bl-B.immilus BS-30 kGy
^,-AVA 6-60 Bl-B.pumiiw WS-30 kGy
WA 6-60 lil-B.pumiliis ODT-30 kGy
^VA 6-60 Bl-B.ptimilus OLT-30 kGy
y ""W A 6-60 81-B.piunilux FS-30 kGy
^ AV A 6-60 Bl ~Brpitmilus MS-30 kGy
6-60 Bl-B.pumtlus PBS-30 kGy
6-60 Bl -B.pumilus BS-30 kGy
^--•WA 6-60 Bl-B.pumitus PS-30 kGv
6-60 B\-Bptumtm OPS-30 kGy
6-60 Bl-B.pumilus LS-30 kGy
s*
-------
Biological Indicator Results Sheet Page
Test Information ' ?
EPA Piojecl No
6-60
rhmctans Name
Nicole Griffin Gatchalian
reorders Name
Nicole Griffin Gatchalian

Shannon Sera
Test Date
*26/2
Bis into TSB
'27/2015
fesi No
Is for WA 6-60 irradiated at 30 kGy
B! Lot NufTsbei/Expiration Date
iphaeus tot 1163211 Exp 1/17
Bl Lot Number/Expiration Date
8 pumilus Lot 716?Q7 Exp 10/16
¦' ¦ Results 	
Dale
1/2015
6'5'2015
6/5/2015

tube Result
Plate Result
Final Result
Sample l[



WA 6-60 BI-8. ntrophaeus DS-30-kGy
\r
* ' '
t.r
WA8-60BI-S atmphaeus WS-30-kGy
, t
(M
V
f\l

WA 6-60 BI-8 Mophaetm ODT-30-kGy
t4
J.4
.. k\
WA 6-60 Bl-S atiophaeos OLT-30-kGy
		 - .11. .
r\j

WA 6-60 BI-8 auopftneus FS-30-kGy


:'v. ..
WA 6-60 Bl-S atiophaeus MS-30-kGy



WA 6-60 Bl-S atiophaeus PBS-30-kGy
. f:.J . ..
i'J
N
WA 5-60 8!»B atiophaeus BS-30-kGy
•Si

(-!
WA 6-60 Bl-B attopbamis PS-30-kGy
, w.. . ,
ii
	('-Si	
f .J
WA 6-60 Bl-S atrophaeus OPS-30-kGy
nT
N
}\I
WA 6-60 Bl-B atiophaeus LS-30-kGy


Ki
WA 6-60 BI-6 atrophaeus PPS-30-kGy
, l\\,
>,Xi
K,
WA 6-60 Bl-B pumikis DS-30-kGy
rj
t\ 1
. t-i
WA 6-60 BI-0 pu»h!im WS-30-kGy
V

	 i\) 	
WA 6-60 81-8 pwmitis GDT-30-kGy

i \t
Ni
WA 5-60 Bl-S pumilus OLT-30-kGy
	»N»
, i
* !
WA 6-60 Bl-S pumilus FS-30-kGy
[M
_
a
WA 6-60 Bl-B' pumiius MS-30-kGy
v.
k,
r-4
WA 6-60 Bl-S, pumilus PBS-30-kGy
i-J
f4
r4 .
WA 6-60 Bl-S ptmulus BS-30-kGy


M
WA 6-60 81-6 pumilus PS-30-kGy
;\)
, ... f\i 	
	M	
WA 6-60 Bl-B pumilus OPS-30-kGy

f-J

WA 6-60 81-0. pumilus LS-30-kGy
fNi
M
Ni
WA 6-60 BI-6 pumiius PPS-30-kGy
t\i
	_ „
N
WA 6-60 Bl-S, atiophaeus postt>\e '

Cz
,
WA 6-60 81-8 atrophaeus positive 2

"L

WA 6-60 81-6 attopiumm positives

o
ft
WA 6-60 Bl-S pumilus positive 1

($
(' *
WA 6-60 Bl-S pumilus positive 2

(h
C^'
WA 6-60 Bl-B pumilus positive 3
( •
(r
i:J
TSB Neg 1
. _	hi	
r____
w
TSB Neg 2
tvl
i\f
k*
"Si: -
	H	

i N!
ISA h.

N
Ki
TSA Nea 2

Ih
Ni
ISA Neg 3

M

Initials of analyst
li- "» *

"K -• s\ ,-r-
\ sJ!T
Key
G = growth
N = no growth
99
-------
Cp
Fn \
ARCADIS
4915 Prospectus flrhra
Durham, MC 27713
1919) 541-3662
FAX(919)544-5690
Page 1
Report lo;
lliirham Wyrzykowska-
Ccrsidini
Bill lo:
Nol Applicable
PROJECT NUMBER
RN990276.0060
1*0#
Not Applicable
Chain of Custody Record
PROJECT NAME Material Compatibility of Gamma Irradiation
COI l i t II IIIIV Wfr VI It Mr
ftarba ra Wyrzykows ka-Ccrad in i
FIELD
SAMPLE II)
WA 6-60 Bl-/?. alntphuvus DS-50 kGy
WA 6-60 BI - fi. airophaei/s WS-50 kGy
WA 6-60 m-fi.arwpftaitux ODT-50 kGy
WA 6-60 Dl -/?, citropJweus OLT-50 kGy
WA 6-60 Bl - fi. utrophaeus l'S-50 kGy
WA 6-60 IM-fi.atrophacm MS-50 kGy
WA 6-60 m-B tarophavus PBS-50 kGy
WA 6-60 lil'IJ.tilroplMt'ux US-50 kGy
WA 6-60 BJ-5. atropfKWttS PS-50 kGy
WA 6-60 m-R alroptMcus OPS-50 kGy
WA 6-60 \&]-li.alrt)pfitntux l.S-50 kGy
WA 6-60 [M'li.alrophiu'iis PPS-50 kGy
RUSH
FACTOR
I week
1A7
t w eek
TAT
I week
TAT
I week
TAT
I week
TAT
I week
TAT
I week
TAT
I week
TAT
I week
TAT
I week
TAJ,
I week
TAT
I week
TAT
SAMPLE
.Matrix
01 test strip
ATCC « 9372
111 test strip
A ICC tf 9372
1)1 test strip
ATCC tt 9372
Bl lest strip
ATCC tf 9372
Bl lest strip
ATCC // 9372
Bl lest strip
ATCC o 9372
Bl lest strip
ATCC # 9372
Bl lest strip
ATCC * 9372
Bl test strip
ATCC * 9372
Bl lest strip
ATCC * 9372
Bl lesi strip
ATCC « 9372
Bl lesi strip
ATCC U 9372
DATE/
TIME
5/28 15
5/28/15
5/28 15
5/28/15
3/28/15
5/28 15
5/28/15
5/28 15
5/28/15
5/28/15
5/28/15
5/28/15
Analysis
Required
Qualitative
Qualitative
Qualitative
Qualitative
Qualitative
Qualitative
Qualitative
Qualitative
Qualitative
Qualitative
Qualitative
Qualitative
I.AHORVI'OKt:
Omitc Microbiology Laboratory do Nicole Griffin Gaichalian
I VB \P DRESS
EPA/HIP N(
REPORT FORMA I (CIRCLEALL RLQl tRU»
UK 1 KOMC A ERI! U I A\ II \UIK iW\
REMARKS
Documents test set Bl 1,
irradiated at 30kGy
Wood test set Bl 1,
irradiated al 50 kGv
Oil dark surrogate lest set
Bit, irradiated at 50 kGy
Oil light surrogate test set
Bl 1, irradiated at 50 kGy
Fabric test set Bl I.
Irradiated al 50 kGy
Metal lest sei Bl I.
Irradiated al SO kGy
Porcelain tesi set Bl I.
Irradiated .u 50 kG\
Books test sic Dl I.
Irradiated at 50 kGy
Photographs test set Bl
Irradiated at 50 kGy
Oil historical test set Bl I
Irradiated at 50 kGy
Leather lest set Bl \,
Irradiated at 50 kGy
Pastel test set Bl I.
Irradiated at 50 kGy
(_ov	exp i/n-
AIt ll> NO
liar l*h
100
-------
Ff#Aj
ARCADIS
WA 6-60 Bl-Bpumilus DS-50 kGy
1 week
TAT
Bl test strip
ATCG # 27142
5/28/15
1
Qualitative
Documents test set BI2,
irradiated at .lOkGv

WA 6*60 Bl-B.pumilus WS-50 kGy
1 week
TAT
Bl lest strip
ATCC # 27)42
5/28/15
t
Qualitative
Wood test set BI2t
irradiated at 50 kGy
WA 6-60 B\-B.pumilus ODT-50 kGy
1 week
TAT
Bl lest strip
ATCC #27142
5/28/15
t
Qualitative
Oil dark surrogate lest set
B12, irradiated at 50 kGy
WA 6-60 m-Bpumitm Ol.T-50 kGy
1 week
TAT
Bl test strip
ATCC # 27142
5/28/15
!
Qualitative
Oil light surrogate test set
BI2. Irradiated at 50 kGy

WA 6-60 m-Bpitmilus FS-50 kGy
1 week
TAT
Bl test strip
ATCC a 27142
5/2815
1
Qualitative
Fabric test set B12.
Irradiated at 50 kGy

WA 6-60 Bl-B.pumiius MS-50 kGy
1 week
TAT
Bl test strip
ATCC #27142
5/28/15
(
Qualitative
Metal test set B12,
Irradiated at 50 kGy
WA 6-60 &l-f!puinilus PBS-50 kGy
t week
TAT
Bl test strip
ATCC « 27142
5/28/15
I
Qualitative
Porcelain lest set BI2,
Irradiated at 50 kGy

WA 6-60 Bl'B.puniflm BS-50 kGv
1 week
TAT
Bl test strip
ATCC # 27142
5/28/15
t
Qualitative
Books test ste BI2,
Irradiated at 50 kGy

WA 6-60 Bl-B.pumilus PS-50 kGy
1 week
TAT
Bl test strip
ATCC # 27142
5/28/15
i
Qualitative
Photographs test set B12.
Irradiated at 50 kGv

WA 6-60 BUB.pumilus OPS-50 kGy
1 week
TAT
Bl test strip
ATCC H 27142
5/28/15
1
Qualitative
Oil historical test set 1)12.
Irradiated at 50 kGy

WA 6-60 Bl-B.pttmilus LS-50 kGy
1 week
TAT
Bl test strip
ATCC #27142
5/28/15
l
Qualitative
Leather test set BI2,
Irradiated at 50 kGy

WA 6-60 Bl•B.pumilus PPS-50 kGy
1 week
TAT
Bl lest strip
ATCC #27142
5/28/15
I
Qualitative
Pastel test set BI2,
Irradiated at 50 kGy





REMARKS
His (Ilafraphaeus test strip Log 6 and B.pumilus lest strip
I hg 6) lor WA 6*60 samples, irradiated at 50 kGy Do not
refrigerate.
RELINQUISH
ED BY:
3MC
DATE
Si2 putvilu,5 Ufl- 1-lb'T-CiT- £xp
101
-------
Biological Indicator Results Sheet Page i of 1 ]( It-'
Test Information " \
EPA Project No,
6-60
Technicians Name
Nicole Griffin Gatchaliart
Recorders Name
Nicole Griffin Gatchalian
PI
Shannon Serre
Test Date
5/28/2015
Bis into TSB
5/28/2015
Test No.
Bis for WA 6-60 irradiated at 50 kGy
Bl Lot Number/Expiration Date
B. atrophaeus Lot 1163211 Exp 1/17
Bl Lot Number/Expiration Date
8. pumilus Lot 716707 Exp 10/16

Date
6/4/2015
OlUlAU io
6/5/2015

Tube Result
Plate Result
Final Result
Sample ID



WA 6-60 Bl-S. atrophaeus DS-50-kGy
fM
f\)
Ki
WA 8-80 Bl-S. atrophaeus WS-50-kGy
fc?
(-1

WA 8-60 Bl-S, atrophaeus ODT-50-kGy
f\j
(si

WA 6-60 Bl-S atrophaeus OLT-50-kGy

(-c

WA6-60 Bl-S atrophaeus FS-50-kGy
N
hi

WA 6-80 Bl-S. atrophaeus MS-50-kGy
f\i
l\J
Ki
WA 6-60 Bl-B. atrophaeus PBS-50-kGy
N

ki
WA 8-60 BI-8. atrophaeus BS-50-kGy
N
Ki
Ki
WA 6-60 BI-8, atrophaeus PS-50-kGy
|\i
|\i

WA 8-80 Bl-B. atrophaeus OPS-50-kGy

H
fvi
WA 8-60 Bl-B. atrophaeus LS-50-kGy
i\i
Ni
f\i
WA 6-60 Bl-B. atrophaeus PPS-50-kGy

<~

WA 6-60 BI-6. pumitus DS-50-kGy
K.!
M
KS
WA 6-60 Bl-B. pumitus WS-50-kGy
n)
N
Ki
WA 6-80 Bl-e. pumitus ODT-50-kGy
ivi
[\j
f\]
WA 6-60 Bl-B. pumitus OLT-50-kGy

Ns
i\'s
WA 8-60 BI-6, pumitus FS-50-kGy

txi
tO
WA 8-80 Bl-S pumitus MS-50-kGy
t\J
fvJ
k;
WA 8-60 Bl-B. pumitus PBS-50-kGy
|\i
(\i
Ki
WA 6-60 BI-6. pumitus BS-50-kGy
fvi
Ki
Ki
WA 6-80 Bl-S. pumitus PS-50-kGy
		rn			
i\S
t\j
fvi
WA 6-60 Bl-e. pumitus OPS-50-kGy
N

K\
WA 6-60 Bl-S pumilus LS-50-kGy
f -r*

t T
WA 6-60 Bl-B pumitus PPS-50-kGy
N

kl
WA 6-60 Bl-B, atrophaeus positive 1
(,fy
i....,?
(~
WA 6-80 BI-6, atrophaeus positive 2
C-y
fr<
Ln"
WA 6-60 Bl-S. atrophaeus positive 3


( -v
WA 6-60 Bl-B. pumitus positive 1
{;*

f t
WA 6-60 Bl-B. pumilus positive 2
I?
O

WA 6-60 Bl-B pumilus positive 3

&
. .. O
TSB Neg 1
	
fvi .. ..
|\>
TSB Neg 2
I\l
t\i
W
TSB Neq 3

(Vi
Ivi
TSA Neg 1
>ilH -a!

i\t
TSA Neg 2
-44-
f\j
r4
TSA Neg 3
-------
vvEPA
United States
Environmental Protection
Agency
PRESORTED STANDARD
POSTAGE & FEES PAID
EPA
PERMIT NO. G-35
Office of Research and Development (8101R)
Washington, DC 20460
Official Business
Penalty for Private Use
$300
-------