EPA-600/2-83-067
August 1983
WASTEWATER TREATMENT
WITH PLANTS IN NUTRIENT FILMS
by
W. J. Jewell
J. J. Madras
W. W. Clarkson
H. DeLancey-Pompe
R. M. Kabrick
Department of Agricultural Engineering
Cornell University
Ithaca, New York 1U853-0317
U.S. Znvironuienxa.1 Protection Agency Grant R807134-01-0
Dr. Willian R. Duffer, Project Officer
Robert S. Kerr Environmental Research Laboratory
\da, Oklahoma 7^820
U.S. Department of Interior
Office of Watei Research Technology Research Grant
Dr. J B. Marvin, Project Manager
Office of Water Research and Technology
Department of Interior
Washington, D.C. 202^0
Cornell University, New York State College of Agriculture and Life Sciences,
a Statutory College of the Stax.R Tim"of New York

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TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing)
1. REPORT NO. 2.
EPA-600/?-R3-0fi7
3. RECIPIENT'S ACCESSIONED-. ^
?®3 5 ^
4. TiTLE AND SUBTITLE
WASTEWATER TREATMENT WITH PLANTS IN NUTRIENT FILMS
5.	REPORT DATE
August 1983
6.	PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
W.J. Jewell, J.J. Madras, W.W. Clarkson, H. DeLancey-
Pompe, and R.M. Kabrick
8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Department of Agricultural Engineering
Cornell University
Ithaca, New York 14853
10. PROGRAM ELEMENT NO.
•CAZB1B
11. CONTRACT/GRANT NO.
CR807134
12. SPONSORING AGENCY NAME AND ADDRESS
U.S. Environmental Protection Agency
Robert S. Kerr Environmental Research Laboratory
P.O. Box 1198
Ada, Oklahoma 74820
13. TYPE OF REPORT AND PERIOD COVERED
Final 10/79 - 06/82
14. SPONSORING AGENCY CODE
EPA-600/015
15. SUPPLEMENTARY NOTES
16. ABSTRACT
The nutrient film technique (NFT) is a unique modification of a hydroponic plant
growth system which utilizes plants growing on an impermeable surface. A thin film
of water flowing through the extensive root system provides nutrients for plants and
associated microbial growth. Root masses up to 15 cm thick or more have been obtained
This self-generating plant system could be used as a filter to immobilize and use the
gross and trace organics in wastewater. The goal of this study was to determine the
economic, technical, and practical feasibility of using plants grown in the NFT
system as pollution control systems.
NFT systems appear capable of providing secondary quality treatment with some
nutrient removal on a relatively small area compared to overland flow systems. At
loading rates of 10 cm per day the effluent quality with primary settled sewage was
often less than 10 mg/1 for suspended solids and biochemical oxygen demand. The
influent sewage temperature was 9 C. Estimated area needs of an NFT system designed
for BOD and SS removal appear to be approximately 3 hectares for a community of
10,000 people, whereas up to 10 times this amount may be needed to provide nutrient
control.
17.	KEY WORDS AND DOCUMENT ANALYSIS
a. DESCRIPTORS
b. IDENTIFIERS/OPEN ENDED TERMS
c. COSATI Field/Group
Aquaculture, water pollution
Waste water, waste treatment
Environmental engineering, hydroponics
Nutrient film technique
06F
68D
98F
13- DISTRIBUTION STATEMENT
RELEASE TO PUBLIC
19. SECURITY CLASS (This Report)
UNCLASSIFIED
21. NO. OF PAGES
626
20. SECURITY CLASS (This page)
UNCLASSIFIED
22. PRICE
EPA Form 2220-1 (9-73)

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DISCLAIMER
Although the research described in this article has been funded wholly
or in part by the United States Environmental Protection Agency through
cooperative agreement number R807134 to Cornell University, it has not been
subjected to the Agency's required peer and policy review and therefore does
not necessarily reflect the views of the Agency and no official endorsement
should be inferred.
i i

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FOREWORD
EPA is charged by Congress to protect the Nation's land, air, and water
systems. Under a mandate of national environmental laws focused on air and
water quality, solid waste management and the control of toxic substances,
pesticides, noise, and radiation, the Agency strives to formulate and imple-
ment actions which lead to a compatible balance between human activities and
the ability of natural systems to support and nurture life. In partial re-
sponse to these mandates, the Robert S. Kerr Environmental Research Laboratory,
Ada, Oklahoma, is charged with the mission to manage research programs: to
investigate the nature, transport, fate, and management of pollutants in ground
water; to develop and demonstrate technologies for treating wastewater with
soils and other natural systems; to control pollution from irrigated crop and
animal production agricultural activities; and to develop and demonstrate
cost-effective land treatment systems for the environmentally safe disposal of
solid and hazardous wastes.
The nutrient film technique (N'FT) is a unique modification of a hydroponic
plant growth system which utilizes plants growing on an impermeable surface.
This is the first comprehensive report on this unique plant production system
which could lead to a new solar-powered pollution control/resource recovery
system. It could serve as a major tool in achieving high water quality and
resource recovery for the future. A thin film of water flowing through the
extensive root system provides nutrients for plants and associated microbial
growth in an i;FT system. Root masses up to 15 cm thick or more have been
obtained. This self-generating plant system could be used as a filter to
immobilize and use the gross and trace pollutants in wastewater. The goal
of this study was to determine the economic, technical, and practical feasi-
bility of using plants grown in the '!FT system as pollution control systems.
In general, the results show that the I'iFT would be highly competitive
with existing secondary treatment alternatives and may significantly reduce
the cost of tertiary treatment where high value plants can be grown and
marketed. The iiFT expands the choice of plants to all species to accomplish
specific pollution control tasks. Roughing plants such as cattails may be
chosen for use in secondary treatment, whereas ornamentals such as roses
may be employed in tertiary treatment sections.
Clinton li. Hall, Director
Robert S. Kerr Environmental Research
Laboratory
iii

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ABSTRACT
The present directions in wastewater treatment should emphasize maximum
pollution control and the recovery of nutrients with minimum capital and
energy investments. This is the first comprehensive report on a unique
plant production system which could lead to a new solar-powered pollution
control/resource recovery system. It could serve as a major tool in
achieving high water quality and resource recovery for the future.
The nutrient film technique (NFT) is a unique modification of a hydroponic
plant growth system which utilizes plants growing on an impermeable
surface. A thin film of water flowing through the extensive root system
provides nutrients for plants and associated microbial growth. Root masses
up to 15 cm thick or more have been obtained. This self-generating plant
system could be used as a filter to immobilize and use the gross and trace
organics in wastewater. The goal of this study was to determine the
economic, technical, and practical feasibility of using plants grown in the
NFT system as pollution control systems.
Three parallel testing programs were used: pure substrate to define
candidate species culturing practices and nutrient responses, synthetic
wastewater, and primary settled domestic sewage. These three substrates
allowed measurements of gross pollutant removal, as well as nutrient and
trace organic removal kinetics. The three testing systems were operated at
application rates of 2.5 to 40 cm per day, and flow rates of more than
11,000 liters per day. Major experimentation was conducted over a two-year
period.
The successful cultural experiments have produced, from seed or cuttings,
viable stands of reed canary grass, coastal bermuda grass, watercress,
napier grass, and cattails in a maximum of six weeks. The propagation of
several species in NFT units and their use in solar greenhouses that require
little, if any, energy input anywhere in the U.S. have been successful.
Many species were screened to identify their possible roles in NFT systems,
and several plants or plant types were found to perform well in the various
parts of an NFT treatment system.
Although the NFT systems evapotranspired large quantities of water, energy
costs for the recovery of this high quality water were prohibitive for the
recovery systems examined.
NFT systems appear capable of providing secondary quality treatment with
some nutrient removal on a relatively small area compared to overland flow
systems. At loading rates of 10 cm per day the effluent quality with
iv

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primary settled sewage was often less than 10 mg/£ for suspended solids and
biochemical oxygen demand. The influent sewage temperature was 9°C.
Estimated area needs of an NFT system designed for BOD and SS removal appear
to be approximately 3 hectares for a community of 10,000 people, whereas up
to 10 times this amount may be needed to provide nutrient control.
Empirical relationships for the removal of nutrients and contaminants were
developed, and would be useful in designing an NFT system for domestic
sewage. Attempts to summarize the results in a kinetic model were generally
unsuccessful. The system also proved effective in removing heavy metals and
trace organics from wastewater.
These area requirements for secondary treatment would result in a cost-
competitive system without taking advantage of any of the reduced operation
costs or commercial benefits of plant production. The area requirement for
tertiary treatment indicates that the NFT would not be competitive with
conventional technologies unless plant products have a commercial value.
This report was submitted in fulfillment of Contract No. R807134-01-0 by the
Department of Agricultural Engineering, Cornell University, New York State
College of Agriculture and Life Sciences, a Statutory College of the State
University of New York, under the sponsorship of the U.S. Environmental
Protection Agency and the U.S. Department of Interior. This report covers
the period October 1, 1979 to June 30, 1982, and work was completed as of
September 30, 1982.
v

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CONTENTS
Page
Foreword		iii
Abstract		iv
Figures		xi
Tables		xx
Abbreviations and. Symbols				xxiv
Acknowledgment				xxv
I. INTRODUCTION 		1
A. Goals and Objectives	•	1
3. Basic Concept and Purpose of the System 		2
C.	Hypothesized Nutrient Film Wastewater Treatment
System 		2
D.	Similarity with Other Treatment Systems and
Adaptation of Hydroponic Systems		i+
E.	Limitations to the NET Wastewater Treatment		6
II. CONCLUSIONS 		7
III. RECOMMENDATIONS		12
A.	Additional Species Screening and Parametric Evaluation	12
B.	Pollutant Removal Mechanisms 		12
C.	Aeration Due to Capillary Mat in Reed-type Plants. . .	12
D.	Lower Nutrient Effects on Yield		13
E.	ET Recovery		13
F.	Drinking Water and Polishing Applications		13
G.	Nitrogen Cycle Manipulation With the NFT		13
H.	Scale-up of Optimum NFT Systems		j-3
IV. BACKGROUND AND LITERATURE REVIEW		1^
A.	Hydroponics		1°
B.	Wastewater and Water Treatment in Hydroponic Systems .	18
C.	Plant Physiology and Morphology Implications 		22
C.l. Redox Potential and the Effect of Sewage Film
on the Plant Root Environment		23
C.2. Physiological Implications of Growth in Sewage
Film		25
C.2.a. Plant Response to Anaerobiosis 		25
C.2.b. Exogenous Phytotoxins 		25
C.2.c. Plant Growth in Sewage Compared to Natural
Habitats		33
vi

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Pags
C.3. Plant Adaptation to Sewage 		37
C.3-a. Mechanisms of Adaptation 		37
C.3.b. Indicators of Adaptation to Anaerobic
Conditions		UO
C.3.c. Identification of Plants Tolerant to NFT
Sewage Environment		^1
C.U. Environmental Factors Affecting Tolerant Plants .	Ul
C.U.a. Effect of Water Level on Growth of Emergents.	kl
C.U.b. Effects of Temperature on Emergents 		U9
C.U.c. Light Intensity 		50
C.U.d. Photoperiod 		51
C.5. Plant Growth Characteristics 		51
C.5»a. Assimilation of Nutrients and Yield 		51
C.5«b. Release of Organic Carbon by Emergents ...	55
C.5«c. Life Histories		58
C.5-d. Effects of Harvesting Yield and Chemical
Composition		58
C.5-e. Regeneration and Reproduction 		58
C.6. Metabolic Activity of Bacteria on Roots 		59
V. SCOPE AND METHODOLOGY		60
A.	Participants in the Project		60
B.	Methods to Meet the Project's Objectives		60
C.	Phase 0 Activities		6l
C.l. Cornell University		6l
C.2. New Hampshire		6U
C.3.	General Electric 		6U
D.	Phase 1 Activities	
D.l.	Cornell University 		64
D.2. CRREL 		65
VI. MATERIALS AM) METHODS		66
A. Process Definition with Synthetic Sewage—Studies
at Ithaca, New York			66
A.l. Synthetic Sewage Composition		66
A.2. Pilot Scale Units (Bracehouse)		69
A.2.a. Brace greenhouse and test units 		69
A.2.b. Loading Rate Schedule and Plant Species
Tested		79
A.2.c. Root Analysis		S3
A.3. Bench Scale Units		^6
A.3.a. Ken Post Greenhouse and Test Units		86
A.3.b. Loading Rates Schedule and Plant Species
Tested		^9
A.1+. Analytical Methods		91
.vii

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Page
B.	Process Definition with Sewage—Studies at Hanover,
NH, and Cayuga Heights, NY		93
B.l. Pilot Scale Units 		93
B.l.a. CREEL Greenhouse and Test Units 		93
B.l.b. CRREL Loading Rates Schedule 		96
B.l.c. Hanover Greenhouse and Test Units 		98
B.l.d. Hs.nover Loading Rates Schedule and Plant
Species Tested		10U
B.l.e. Root Analysis		105
B.l.f. CRREL Analytical Procedures 		105
B.2. Bench Scale Units 		105
B.2.a. Hanover Bench Scale Units and Experiment
Schedule		105
B.2.b. Cayuga Heights Greenhouse, Bench Scale Units
and Experiment Schedule		107
B.3.	Comparison of Synthetic and Actual Domestic
Wastewater		113
C.	Testing Approach 		116
C.l.	Parameter Evaluation 		116
C.2. Season, Temperature and Environmental Controls .	116
C.3. Test Parameters and Duration		117
C.U.	Loading Rate Characterization		11 fl
D.	Plant Considerations 		125
D.l.	Plant Selection Criteria 		125
D.2. Species Tested		132
D.3. Culturing Considerations		133
D.3.a. Obtaining Plant Material 		133
D.3.b. Growing Plant Material 		137
D.U.	Plant Care During Crisis		138
E.	Analytical Approach 		138
E.l.	Computerized Summary of Information 		138
E.2. Long-term Analysis		138
E.3. Sensitivity of System to Controlling Parameters .	139
E.U. Formulation of Predictive Relationships 		139
VII. RESULTS		1U0
A.	Chronological Development of Treatment Capacity of
NFT Systems		1^1
B.	Characteristics of the NFT in Treating Synthetic
Wastewater and Domestic Sewage 		1^7
B.l. pH		1^7
B.2. Dissolved Oxygen 		151
3.3. Temperature		15^
B.U. Suspended Solids . 			15^
B. 5 • Turbidity		156
3.6. Chemical Oxygen Demand 		156
3.7 Nitrogen		163
B.8. Phosphorus		163
viii

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Page
B.9.	Indicator Organisms 		169
3.10. Zinc and Cadmium		169
C.	Process Sensitivity and Pollutant Removal Mechanism
Definition		169
C.l.	Hydraulic Retention Time Effects 		169
C.2. Loading Rates Effects 		173
C.3. Loading Rate Relationships with Synthetic
Wastewater		IT 6
C.U. Primary Settled Domestic Sewage Loading Rates
Effects		180
C.5. Plant Environmental Factors 		196
Co. a. Temperature		198
C.6. Root Mass Influence		198
C.7- Bench Scale Simulation 		199
C.8. Experiments with Recirculating Bench Scale Models	201
C-9- Domestic Sewage, Bench Scale Test 		207
C.10.	Testing of Optimized Design 		211
D.	Water Balance and Energy Transfer 		215
D.l.	ET Rates		216
D.2. ET Recovery Systems		219
D.2.a. Necessary Cooling Capacity 		220
D.2.b. Air-to-air heat exchanger 		220
D.2.c. Refrigerative Dehumidification 		22U
D.3. Economic Considerations 		225
D.U. Alternative Approaches for Condensation 		225
E.	Nutrient Uptake		225
F.	Plant Culturing and Propagation for Establishing
and Operating an NFT System		236
F.l. Culture of Species in Total Controlled
Environments		236
F.l.a. Nutrient Removal in Total Controlled
Environmental Greenhouses 		237
F.l.b. Biomass Production 		237
F.2. Culture of Species in Greenhouses		2U5
F.2.a. Response to Non-carbonaceous Nutrient
Solution		245
F.2.b. Plant Response to Sewage in NFT Systems . . .	249
F.3. Diagnosing and Treating Plant Growth Problems . .	257
G.	Toxic Constituent Considerations—Cadmium and Toxic
Organics (Volatile and Nonvolatile) 		262
H.	Comparison of Synthetic and Domestic Sewage in
NFT Systems		262
I.	Development of Predictive Relationships 		267
VIII. DISCUSSION		281
A. General Considerations		28l
ix

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Pase
B.	NFT Characteristics and Pollutant Removal
Mechanisms		282
B.l. Settling, Filtration, and Suspended Solids
Control		282
B.2. Microbial Metabolism and BOD and COD Removal . . .	28U
B.3. Nutrient Removals 		285
C.	Toxic Substances 		288
D.	TDS Manipulation		288
E.	Temperature Effects 		290
F.	Process Sensitivity and Design 		290
G.	Plant Environment Factors		291 '
H.	Optimized Facility Considerations		292
I.	Plant Culturing and Propagation		293
IX	NFT TOTAL SYSTEM FEASIBILITY CONSIDERATIONS		295
A.	The NFT As a Secondary Treatment System		295
B.	The NFT As a Tertiary Treatment Facility		298
REFERENCES		300
APPENDICES
A.	General Electric Phase 0 Data		312
B.	Hanover, New Hampshire, Data		315
C.	Cornell University Data		392
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FIGURES
Number	Page
1.1	The nutrient film technique variation of hydroponic
plant production systems 		3
1.2	Schematic diagram showing three-stage hypothesized NFT
treatment system for domestic wastewater with functions
for each stage and fate of all materials		5
U.l	Plymouth, England, research group facilities 		19
4.2	Approximate critical thresholds for stability of redox
systems		2b
4.3	Summary of physiological effects of the flooded root
zone		29
4.4	Aboveground nitrogen content of emergent aquatic plants .	53
4.5	Aboveground phosphorus content of emergent aquatic plants	5^
4.6	Peak aboveground biomass of emergent aquatic plants in
natural habitats 		56
4.7	Peak belowground biomass of emergent aquatic plants in
natural habitats 		57
5.1	Chronology of major events and testing periods of the
NFT project		62
5-2 Different NFT systems at Cornell University, General
Electric Company Syracuse facility, and at CRRE1 in
Hanover, New Hampshire		63
6.1	The Cornell Brace-style solar greenhouse 		JO
6.2	Schematic diagram of basic Cornell NFT experimental unit.	J2
6.3	Standard NFT tray		73
6.4	Section view of NFT tray, sampling ports, and adjustable
support structure 		7^
xi

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Number	Page
6.5	Level unit that can withstand, flooding		75
6.6	View of interior of Cornell solar greenhouse showing NFT
trays emptry and containing reed canary grass 		75
6.7	Schematic diagram of the influent distribution box. ...	77
6.6	View of synthetic wastewater mixing and distribution
system		78
6.9	Schematic diagram of three-part 36.6 meter system at
Hanover containing several species 		8l
6.10	Schematic diagram of Bracehouse 36.6 meter multi-
species unit		82
6.11	Root sampling devices: (a) cattail, bulrush and
phragmi tes, (b) reed canary grass		8U
6.12	Post House 3 meter (10 ft) units in operation		87
6.13	Construction of 3 meter (10 ft) units in Post House ...	87
6.1k Schematic of Post House 1.5 meter (5 ft) unit	 88
6.15	Post House 1.5 meter (5 ft) unit in operation		88
6.16	Superstructure and plywood covered floor schematics of
5.1 meter (20 foot) portable units		90
6.17	Experimental JJFT unit for primary settled domestic
sewage studies at CEREL		9^
6.18	View of initial placement of reed canary grass washed
sod just after the first harvest at CREEL		9 k
6.19	Schematic diagram of the CRREL NFT system		95
6.20	Site plan of greenhouse and Hanover, New Hampshire,
sewage treatment plant 		99
6.21	Attachment of trusses to posts during construction of
the Hanover greenhouse		101
6.22	View of the plastic covered Hanover greenhouse, treatment
plant building, and surrounding grounds 		101
6.23	Schematic diagram of units in the Hanover greenhouse. . .	102
6.2k The interior of the Hanover greenhouse showing the NFT
trays under construction		103
xii

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Number	Page
6.25	Test unit used in the high loading rate experiments
using domestic sewage at the Cayuga Heights treatment
plant	 109
6.26	Napier grass used in high loading experiments at the
Cayuga Heights treatment plant	 110
6.27	Schematic diagram of experimental system used for high
loading rate experiments at the Cayuga Heights treatment
plant	 Ill
6.28	Plastic shelter and enclosed units at the Cayuga Heights,
New York, treatment plant 	 112
6.29	Hydrograph for 36.6 meter Bracehouse pilot system on
i+-27-8l (10.2 cm per day, loaded during first 7-5 minutes
of each half hour) 		122
6.30	Direct measurement of flow entering and leaving various
units in Bracehouse system on May 27, 198l	 123
6.31	Relation between the number of species included in an
end product and the relative value of that end product. .	131
6.32	Stand of phragmites at Montezuma Wildlife Refuge	 13^
6.33	White canary grass roots forming a root mat below
capillary matting used in seeding trials		136
6.3^ Napier grass, sugar cane and cottonwood cuttings after
placement in the NFT 3 meter units		136
7.1	Influent and effluent chemical oxygen demand for the
Cornell NFT treating synthetic wastewater in spring 1981	142
7-2	Chronological data development for Cornell Phase I (1981)
studies with reed canary grass NFT units	 1^3
7.3	Influent and effluent chemical oxygen demand for the
Hanover NFT treating domestic wastewater in spring 1981 . 1*+U
1.k	Example of chronological data development for domestic
sewage testing with the NFT at Hanover, New Hampshire . . 1^5-1^6
7.5	Incident radiation and yield of reed canary grass grown
in continuing NFT culture in the Cornell Bracehouse
over the course of experimentation 	 1U8-150
7.6	Dissolved oxygen concentration in reed canary grass NFT
treating synthetic wastewater 	 152
xiii

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Number	Page
7.7	Dissolved oxygen concentration in multispecies NFT unit
treating primary settled domestic sewage at Hanover,
New Hampshire		153
7.8	Mean suspended solids concentrations in NFT systems
treating domestic wastewater at 10.2 cm/d	 155
7.9	Reductions of total- and soluble chemical oxygen demand
concentrations in synthetic wastewater through 12 m and
36 m systems	 157
7.10	Chemical oxygen demand removal efficiency at different
stages of stand maturity and under different mean
temperatures 	 158
7.11	Influence of system length on soluble CCD removal from
synthetic sewage at an area loading rate of 20.3 cm/d . . 159
7-12 Reductions of total and soluble chemical oxygen demand
concentrations in domestic sewage in 36 n multiple
species NFT units, which were tested at three different
loading rates 		l60
7.13 Total and soluble oxygen demand concentration at various
points in 36 m multiple species unit treating domestic
wastewater. Three loading rates are shown	 l6l
7.1^ Comparison of COD removal from domestic sewage during
relatively warm and cold-temperatures		 . 162
7.15	Total nitrogen concentration of synthetic wastewater in
three different NFT systems at an area loading rate of
10 cm/d	 164
7.16	Total Kjeldahl nitrogen concentration reductions in 9-3 m2
reed canary grass units using synthetic wastewater. . . . 165
7.17	Ammonia nitrogen concentrations of synthetic sewage at
various sampling points in NFT systems	 l66
7-18 Total phosphorus concentrations of synthetic wastewater
in different length systems 	 167
7.19	Total phosphorus concentrations of domestic sewage at
different loading rates to two systems	 168
7.20	Influent and effluent concentrations of phosphorus in
domestic sewage in the Hanover NFT system, which began
operation on February 16 , 1981	 170
xiv

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Number	Page
7.21 Influent and. effluent zinc concentrations of synthetic
sewage in a reed canary grass pilot scale unit. First
day of analysis was July 25, 1980 	 171
7-22 Influent and. effluent zinc concentrations of synthetic
sewage in a reed canary grass pilot scale unit. First
day of analysis was July 25, 1980 	 172
7.23 Hydraulic retention times of a 36 m NFT system using
synthetic wastewater			 17U
7.2U Hydraulic retention time of the 36 m system using
domestic wastewater loaded at 10.2 cm/d	 175
7.25	Relationship between total COD removal efficiency and
total COD mass loading rate for conditions tested with
synthetic sewage 	 177
7.26	Relationship between total CCD mass loading rate and
total COD mass removal in the NFT systems treating
domestic sewage 	 178
7.27	Relationship between soluble COD mass loading rate and
soluble COD mass removal in the NFT systems treating
synthetic sewage	 179
7.28	Relationship between total nitrogen removal efficiency
and total nitrogen mass loading rate for conditions
tested with synthetic sewage	 l8l
7.29	Relationship between soluble nitrogen removal efficiency
and soluble nitrogen mass loading rate for conditions
tested with synthetic sewage	 182
7-30 Relationship bewteen total phosphorus removal efficiency
and total phosphorus mass loading rate for conditions
tested with synthetic sewage	 183
7-31 Relationship between soluble phosphorus removal efficiency
and soluble phosphorus mass loading rate for conditions
tested with synthetic sewage	 18U
7.32 Relationship between BOD removal efficiency and BOD mass
loading rate for conditions tested with domestic sewage . 185
7-33 Relationship between BOD mass loading rate and BOD mass
removal in the NFT systems treating domestic sewage . . . 186
7.3^ Relationship between TOC removal efficiency and TOC mass
loading rate for conditions tested with domestic sewage .	187
xv

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Number	Page
T-35 Relationship between TOC mass loading rate and TOC mass
removal in the NFT systems treating domestic sewage. . . . 188
7-36 Relationship between suspended solids mass loading rate
and suspended solids mass removal in the NFT systems
treating domestic sewage 	 189
7.37	Relationship bewteen volatile suspended solids mass
loading rate and volatile suspended solids mass removal
in the NFT systems treating domestic sewage	 190
7.38	Relationship between nitrogen mass loading rate and
nitrogen mass removal for conditions tested with domestic
sewage	 191
7'39 Relationship between phosphorus mass loading rate and
phosphorus mass removal for conditions tested with
domestic sewage	 192
7.1+0 Effect of temperature on the treatment efficiency of
BOD and TOC in NFT systems using domestic sewage 	 193
7.4l Effect of temperature on the treatment efficiency of
total and volatile suspended solids in NFT systems
using domestic sewage	 19*+
7.1+2 Effect of temperature on the treatment efficiency of
nitrogen and phosphorus in NFT systems using domestic
sewage	 195
7.1+3 Healthy canary grass in Unit 3, Bracehouse	 197
7.1+1+ Brown canary grass in Unit 3, Bracehouse	 197
7.U5 Hydraulic retention times in the bench scale varied
flow experiment using synthetic wastewater 	 202
7.1+6 Hydraulic volume contained in bench scale varied flow
units at different loading rates of domestic sewage . . . 203
7.1+7 Total and soluble COD concentrations of the influent
and effluent synthetic wastewater to a reed canary
grass bench scale unit loaded at 20 cm/d	 20l+
7.1+8 Effluent biochemical oxygen demand concentrations of
different units in the varied flow experiment, which
began July 20, 1981 	 205
7.1+9 Comparison of evapotranspiration from bench scale units. . 206
xvi

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Number	Page
7.50 Effluent phosphorus concentrations from the control and
napier grass units in an early bench scale experiment
using a recirculating solution. The experiment began
on February 27, 1981 	 208
7ol Effluent phosphorus concentrations from the control
and napier grass units in a later bench scale experiment
using a recirculating solution. The experiment began
on April 8, 1981 	 208
7.52 Effluent total nitrogen concentrations of synthetic
sewage from bench scale units containing several plant
species. This series of experiments began March 18, 1981. 209
7-53 Relationship between area loading rate and removal of
solids and BOD in bench scale NFT systems using synthetic
sewage	 210
7•5^ Effluent biochemical oxygen demand concentrations of
units at different loading rates in the varied flow
experiment using domestic sewage. The experiment
started on June 22, 1981	 212
7.55	Biochemical oxygen demand concentrations over time in
recirculating domestic sewage units containing
different plant species 	 213
7.56	Schematic energy balance for a hypothetical greenhouse
in January and July in Ithaca, New York	 221
7-57 Condensation temperature and cooling capacity necessary
to recover all ET vapor as a function of mass flow rate
of air	 222
7.58	Configuration of the air-to-air heat exchanger used in
design estimates 	 223
7.59	Energy balance for the refrigerative compression system . 226
7.60	Dry weight harvest yield of a 9-3 m2 reed canary grass
control unit	 227
7.61	Plant seedlings growing on mat in NFT trough	 238
7-62 Growth of watercress before biweekly harvest. Plants
grew to 20 cm above bed floor	 238
7.63 Stand of watercress after biweekly harvest 	 239
7.6U Stand of reed canary grass grown from plugs	 239
xvii

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Number	Page
7.65	Napier grass cutting at initial transplant into the
NFT trough		2k0
7.66	Napier grass stand at time of move to larger bed ....	2^0
7.67	Regrowth of napier grass three weeks after first harvest	2Ul
7.68	Side view of root mats of reed canary grass, coastal
Bermuda grass, and watercress grown in NFT trough. . . .	2Ul
7-69 Bottom view of root mats of reed canary grass, coastal
Bermuda grass, and watercress grown in NFT trough. . . .	2U2
7-70 Side view of root mats of cattails and napier grass
grown in NFT trough		2^+2
7.71	Bottom view of root mats of cattails and napier grass
grown in NFT trough		2k3
7.72	Nitrogen and phosphorus removals from General Electric
NFT unit containing reed canary grass		2kb
7-73 Cattail, bulrush, phragmites, and canary grass growing
well in the Bracehouse 36.6 meter system		250
7-7^ Root zone of a rose in peat pot showing anoxic accumula-
tions at bottom				252
7-75 Hanover unit containing cucumbers during early testing
of the large unit		25^-
7.76 Poor root development of cucumber after a month in the
NFT unit in the Hanover greenhouse		25^
7-77 Schematic diagram of second system in Hanover greenhouse
showing locations of plants relative to sewage flow . .	256
7.78	Black root zone below dead reed canary grass		258
7.79	Geranium plant showing new root growth after old roots
had rotted in peat pot		258
7-80 Chrysanthemum and peat pot shewing absence of roots due
to root death		259
J.8l Larval gnats in connecting gutter between trays at the
Hanover greenhouse 		26l
7.82 Cadmium concentrations at various points in three
different length systems, the longest of which contained
several species		26U
xviii

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Number	Page
T.83 Comparison of synthetic and domestic sewage variables
in 2T-9 ni2 units operated ax an area loading rate of
10.2 cm/d	 266
7-8U Comparison of chemical oxygen demand reductions in
domestic and synthetic sewage at various loading rates
in 27.9 units	 268
7-85 Rate determination plot for BOD removal from domestic
sewage by a canary grass system	 273
7.86	Rate determination plot for suspended solids removal
from domestic sewage by a canary grass system		27*+
7.87	Rate determination plot for volatile suspended solids
removal from domestic sewage by a canary grass system . . 275
7.88	Rate determination plot for total nitrogen removal
from domestic sewage by a canary grass system 	 276
7.89	Rate determination plot for total phosphorus removal
from domestic sewage by a canary grass system 	 277
7.90	Rate determination plot for COD removal from synthetic
sewage by canary grass units in different size systems. . 278
7-91 Rate determination plot for phosphorus removal from
synthetic sewage by canary grass units in different
size units	 279
7.92 Rate determination for total nitrogen removal from
synthetic sewage in canary grass units of different
areas	 280
8.1	Photograph of the effluent from an ITFT unit treating
primary settled sewage showing the large coagulated
particles settled in the overflow trough 		286
3.2	Relationship of plant yield to land area required for
complete nitrogen removal from domestic sewage for
crops with two different levels of nitrogen	 287
8.3	Calculated maximum total dissolved salts removal
efficiency with plants in the NFT at varying yields . . . 289
9.1	Schematic of NFT treatment facility capable of treating
domestic sewage from 10,000 people	 296
xix

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TABLES
Number	Page
U.l	State of the Art Characteristics of Aquatic Systems
and Land Application Systems Used for Domestic Sewage
Treatment		15
k.2 Plant Characteristics Important for Wastewater Treat-
ment Mechanisms in an NFT System		22
h.3	Potentially Toxic Elements Found in Residential Trunk
Sewers		30
Essential Minerals for Higher Plants		30
1*0	Amounts of Cadmium Absorbed by Various Plant Species in
Relation to Solution Cadmium Concentration 		31
k.6	Concentration of Fly Ash Pollutants Absorbed by Aquatic
Plants 			32
U.7	Solution Cd Concentrations Producing 50% Growth Reduction,
Visual Symptoms Associated with 50% Growth Reduction, and
Concentration in Leaf at 50% Growth Reduction		33
U.8	Effect of Heavy Metals in the Nutrient Solution on
Growth of Floating or Submergent Aquatic Plants 		3^
U.9	Potential Phytotoxicity of Hydrocarbons and Other
Organic Compounds 	 		36
U.10 Plant Adaptations to Survive an Anoxic Environment. ...	38
l+.ll Emergent Species Tolerant or Potentially Tolerant to
NFT Sewage Environment		^2
U.12 Organic Matter Content of Some Emergents	 ....	52
6.1	Dry Chemical Constitutents in Concentrated Feed Stock
and Synthetic Sewage		67
6.2	Phase 0 Testing Schedule for the Brace House 12.2 meter
(U0 foot) Canary Grass Unit		T9
6.3	Phase 1 Schedule for the 3race House 12.2 meter (U0 foot)
and 2k.k meter (80 foot) Canary Grass Units 		30
xx

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Number	Page
6.1+	Testing Schedule for the Cornell 36.6 meter (120 foot)
Multispecies System 		83
6.5	Cornell Bench Scale Experiments Conducted in the Glass
Covered Greenhouse (Ken Post Laboratory) 		91
6.6	Summary of CRREL Study Activities in the 12.2 m Pilot
Scale System During Phase 0 	 .........	97
6.7	Loading Conditions Tested in the 12.2 m Pilot Scale
System in the CRREL Greenhouse During Phase 1 ..... .	98
6.8	Loading Conditions Tested in the Pilot Scale Unit at
the Hanover Treatment Plant 	 10U
6.9	Hanover Greenhouse Bench Scale Experiments 	 106
6.10	Schedule of Experimental Conditions at Cayuga Heights . . 113
6.11	Characteristics of Influent Synthetic and Real Sewage . . 115
6.12	Test Parameters and Duration of the Cornell Pilot Scale
Systems Using Synthetic Sewage 	 117
6.13	Test Parameters and Duration of the CRREL and Hanover
Pilot Scale Systems Using Domestic Sewage 	 118
6.11+ Comparison of Loading Rate Parameters for Synthetic
Sewage		 120
6.15 Typical Hydraulic Characteristics of a 36.6 m Long
(27.9 m2) Pilot Scale WET Unit as Estimated from Water
Depth Measurements During Operation of the Unit . . , .
12U
6.16	Comparison of Retention Times (in Minutes) in 27-9 m2
Pilot Scale Units Operating at Different Conditions with
Real and Synthetic Wastewater		 . . 126
6.17	Favorable Plant Characteristics for Treatment of Waste-
water in NET Systems	 127
6.18	Plants Grown in NET Units or Examined for Cultural
Properties During the Study 	 132
6.19	Collection Data from Wetland Plants Grown in the 3TFT . . 133
6.20	Volunteer Species Which Survived Transplanting with
Other Wetland Species	 135
6.21	Sources and Propagules of Commercial Plants Grown in NFT
Units			 137
xxi

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Number	Page
7.1	Variables Measured, in Testing Pilot Scale NFT Systems
With Synthetic and Domestic Sewage 	 151
7-2	Range of Pollutant Loading Rates Tested With Synthetic
Sewage in Pilot NFT Units	 176
7.3	Range of Pollutant Loading Rates Tested With Domestic
Settled Sewage in Pilot NFT Units 	 196
7.^ Total and Volatile Accumulations of Materials in the
Roots of Plants Tested in 27-9 ma Pilot Units Using
Synthetic and Domestic Wastewater 	 199
7-5	Total and Volatile Entrapped Solids in the Root Zone
of Plants Tested in 27.9 m2 Pilot Units Using
Synthetic and Domestic Wastewater 	 200
7.6 Summary of Optimized Operation of the NFT as a Primary
and Secondary Combined Treatment System Using
Intermittently Loaded and Unloaded Plants. Experiments
Were Conducted at the Cayuga Heights Village Treatment
Facility	 214
7-7 Evapotranspiration of the 9-3 m3 Reed Canary Grass Unit
of the Cornell Bracehouse. Data Were Collected During
the Testing of the 27-9 m2 Multispecies System in the
Same Greenhouse in 1931	 2l6
7-8 Comparison of Evapotranspiration Between a Canary Grass
Pilot Scale Unit and Bench Scale Units Containing
Different Plant Species at Cornell Using Nutrient
Solution or Synthetic Sewage 	 217
7-9 Comparison of Evapotranspiration Between a 27-9 m2 Pilot
Scale System and Bench Scale Units Containing Different
Plant Species Using Domestic Sewage 	 218
7-10 Dates, Yields, Dry Weights and Nutrient Contents of
Aged Canary Grass Harvests in the Cornell Bracehouse
Unit #2	 228
7.11	Dates, Yields, Dry Weights and Nutrients Contents of
Reed Canary Grass Harvests in the Cornell Brace House
Unit			230
7.12	Dates, Yields, Dry Weights and Nutrients Contents of
Reed Canary Grass Harvests in the Cornell Bracehouse
Unit tf3		232
7.13	Harvest Data from the CRREL Greenhouse Which Grew
Canary Grass in 7-^ m2 Units (Total)		23^
xxii

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Number Page
7.1^	Harvest Data from Hanover Greenhouse in 1981		236
7.15	Grass Nutrient Formula Used in Plant Propagation in
Total Controlled Environment		21+5
7.16	Biomass Yield of Test Species Shoot Harvest 		2k5
7.17	Plant Response to Nutrient Solutions 		2^7
7.18	Plant Response to Sewage			2^9
7.19	Reed Canary Grass Pathologies and Treatments 		257
7.20	Fate of Cadmium in NFT Pilot Systems at Cornell		263
7.21	Trace Organic Concentrations in Sewage in the Hanover
NFT System		265
7.22	System Performance Summary of Pilot Scale Systems Using
Synthetic Sewage 		269
7.23	System Performance of Pilot Scale Systems Using Domestic
Sewage		270
7.2U	Comparison of Mean Mass Removals from Synthetic and
Domestic Sewage in Pilot Scale Systems 		271
7-25 Summary of Substrate Removal Coefficients as Determined
from Assumed first Order Kinetic Estimates for Canary
Grass Systems		272
8.1	Advantages and Disadvantages of the NFT System When
Used for Wastewater Treatment		282
9-1 Summary of Comparison of Two Secondary Treatment Tech-
nologies Compared to an NFT System		297
9.2	Estimated Water Quality to be Produced by the Proposed
Water Reclamation Facility 		299
xxiii

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ABBREVIATIONS AND SYMBOLS
BOD
biochemical oxygen demand
mm
Mn"1^
Mn+1+
millimeter
CaCl2
calcium chloride
manganese
Ca(N03)2
calcium nitrate
manganese
Cd
cadmium
ug
microgram
CdSOi*
cadmium sulfate
mV
millivolts
cm
centimeter
N
nitrogen
C02
carbon dioxide
NaCl
sodium chloride
COD
chemical oxygen demand
nh3
ammonia nitrogen
CRREL
Cold Regions Research and
NO3-
nitrate nitrogen

Engineering Laboratory
02
oxygen
d
day
OM
organic matter
DO
dissolved oxygen
P
phosphorus
ET
evapotranspiration
Pb
lead
ETP
potential evapotranspiration
pco2
partial pressure of CO2
FC
fecal coliform
PO^
orthophosphate phosphorus
Fe ^
Fe+
iron
PPb
parts per billion
ferrous iron
PPm
parts per million
FSS
fixed suspended solids
RBC
rotating biological
g
gram

contactor
ha
hectare
RCG
reed canary grass
HRT
hydraulic retention time
sec
second
H2S
hydrogen sulfide
SOL
soluble
J
joule
SS
suspended solids
JTU
Jackson turbidity unit
Std. Dev.
standard deviation
K
potassium
t
tonne
kg
kilogram
TFS
total fixed solids
K2HPO4
potassium phosphate
TKN
total Kjeldahl nitrogen

dibasic
TN
total nitrogen
kh2po4
potassium phosphate
TOC
total organic carbon

monobasic
TP
total phosphorus
kJ
kilojoule
TSS
total suspended solids
kw
kilowatt
TVS
total volatile solids •
kwh
kilowatt hour
VSS
volatile suspended
£
liter

solids
LR
loading rate
wk
week
m
meter
yr
year
MEQ
milliequivalent
Zn
zinc
MGD
million gallons per day
<
less than

milliliter
>
equal to or greater than
mg ft
milligrams per liter
2T
approximately equal to
xxiv

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ACKNOWLEDGMENTS
This project was supported in part by the U.S. Environmental Protection
Agency Research Grant No. R807134-01-0, Dr. William R. Duffer, Project
Officer, Wastewater Management Branch, Robert S. Kerr Environmental Research
Laboratory, Ada, Oklahoma, and the U.S. Department of Interior, Office of
Water Research Technology, Dr. Jay B. Garvin, Project Manager. Special
thanks are given to our E.P.A. Project Manager and to Mr. James V. Basilico,
U.S. E.P.A. Office of Research and Development, Washington, D.C., for their
interest and guidance provided throughout all stages of this project. They
have provided the critical link required of public project managers to
provide a productive environment to conduct research of the nature sponsored
here. Their recognition of the potential and acknowledgment of the risks
involved are an important component of new efforts such as the NFT process
research.
The authors would like to acknowledge a number of individuals for their
assistance with this study: Professor J. H. Peverly coordinated aquatic
plant screening, selection, collection, and literature review activities.
His continuing support and interest was appreciated. Professor L. D.
Albright provided the Cornell University solar greenhouse space; Professor
R. W. Langhans, J. H. Kurapf, R. W. McBride, D. R. Reniff, and other staff
members at the Kenneth Post Greenhouse Facility provided interest,
encouragement, and research support. Professor R. D. Boyd of Animal Science
allowed use of his atomic absorption unit in this study.
The authors appreciate the support of Mr. Gary Gleason of Gleason
Environmental Processes for his permission and support to conduct
experiments at the Cayuga Heights Village sewage treatment facility in
Ithaca, New York.
Professor Alex G. Alexander of the University of Puerto Rico, Mayaguez
campus, provided sugarcane and napier grass cuttings.
During the early stages of this project the General Electric Company,
Syracuse, New York, was instrumental in developing the project. Thanks go
to Dr. E. G. Scott and Mr. B. G. Vinzant.
During the initial stages of this effort, W. J. Jewell was on
sabbatical leave and located at the U.S. Army Corps of Engineers Cold
Regions Research and Engineering Laboratory (CRREL) in Hanover, New
Hampshire. CRREL's significant, albeit informal, support was very much
appreciated. Special appreciation goes to Dr. K. F. Sterrett, Director of
Research, and D. D. R. Freitag, Technical Director of CRREL, for allocating
xxv

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resources for a portion of this	study. T. Jenkins provided the excellent
toxic information and analysis. P. Butler, D. R. Nelson, and C. Deiner of
CRREL provided sample analysis. J. R. Bouzoun assisted in communication
between research groups.
Activities on this project took place at five different laboratories—
three at Cornell and two in Hanover, New Hampshire. Many individuals at the
CRREL research facility and at Cornell were responsible for the data
development. J. J. Madras coordinated all components of the study, managed
the Cornell experiments, and was instrumental in preparing this final
report. Mr. D. W. Chamberlain, Sewage Plant Superintendent for the Hanover,
New Hampshire, treatment facility provided important assistance in
construction and operation of the full scale greenhouse erected at this
facility. Support and assistance of all personnel at this facility were
appreciated. Hanover's Town Manager, Mr. Peter J. Gartland, made it
possible for this important segment of the project to be completed. Special
recognition for successful conduct of the project goes to M. Arroyo,
S. T. Baker, J. Finn, P. Molitemo, F. E. Mudrak, J. Neander, D. F. Sherman,
J. M. Shortslef, M. Best, E. Moran, and T. Rawinski; D. M. Harbert, who
prepared the manuscript; C. Wong and D. Corwin, who performed computer
analyses; and J. A. Spada, who prepared figures for this publication.
XXV i

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/•
CHAPTER I
INTRODUCTION
The U.S. wastewater management program emphasizes systems that require
minimum capital and energy and recover resources such as nutrients and
water. The U.S. Environmental Protection Agency has made significant
efforts to encourage land treatment and other innovative systems that
support mandates issued by Congress. Similarly, the U.S. Department of the
Interior has fostered water conservation and reuse. The soilless culture
plant production system described here has potential to aid in these pur-
suits. This modification of a hydroponic system, called the Nutrient Film
Technique (NFT), has been developed and tested at significant "proof-of-
concept" scale applications.
A. GOALS AND OBJECTIVES
There is increasing interest in the use of low-cost natural systems that
have existing or built-in pollution control mechanisms. This project
focuses on a new plant production system that could lead to new ways of
treating wastewater and water supplies with a self-generating pollution
control system that could have a number of useful and valuable by-products.
The main goal of this feasibility study was to:
determine the feasibility of using plants as pollutant-
concentrating, pollutant-assimilating and nutrient-
recycling facilities in a unique hydroponic system utiliz-
ing the Nutrient film Technique.
The five specific objectives were to:
1.	define advantages of using plants in the Nutrient Film Technique
system over conventional systems to collect, concentrate, and
assimilate pollutants;
2.	identify research and dvelopment needs to support a long-term
program to define the full potential of the Nutrient Film Technique
system as a pollutant management/resource recovery system;
3.	define the engineering requirements to establish a total
plant-based pollutant control system. This will include area
requirements, hardware, plants and plant production systems;
Cornell University is an equal opportunity}
affirmative action employer and educator.

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4.	gather baseline data on system performance using energy and mass
balance to support econometric data;
5.	determine the feasibility of recovering energy, food, and nutrients
from the plant material.
The NFT system presents opportunities for tailoring a specific plant system
to the treatment job to be accomplished through the choices of plant selec-
tion and system configuration. Design parameters were developed based on
hydraulics and removal of nutrients, organics, trace organics and cadmium.
Plant selection and management methods were investigated and are described.
Finally, the potential evapotranspiration (ET), actual ET and recovery of ET
are discussed.
B. BASIC CONCEPT AND PURPOSE OF THE SYSTEM
The nutrient film technique (NFT) is a unique modification of a hydroponic
plant growth system. The NFT version of hydroponics utilizes plants grown
directly on an impermeable surface to which a thin film of water is continu-
ously applied (see Figure 1.1). The root production on this impermeable
surface will result in a large mass of roots and accumulated matter with a
large surface area. Root masses have been observed to accumulate up to 30
cm deep, separate from the stalk and fruit. The hypothesis here is that
these large masses of self-generating root systems can be used as living
filters. Plant top growth also provides nutrient uptake, shade for protec-
tion from algae, and water removal in the form cf transpiration. Sludge
that would settle in the root filter would be held in place by the roots,
and the filter itself would gradually expand as the sludge accumulated and
occupied more space. After removal of organics and suspended solids, the
remaining refractory soluble organics, nutrients, and remaining toxic ele-
ments would continue to pass through the fine root filter. Since high flow
rates are possible In the absence of large amounts of suspended and organic
matter, subsequent loading rates vould be related to the sorption rates of
critical wastewater constituents su^.h as nutrients. This general inter-
action of pollutants, plants and water leads to a general description of a
hypothesized wastewater treatment system as discussed in the next section.
C. HYPOTHESIZED NUTRIENT FILM WASTEWATER TREATMENT SYSTEM
The fate of pollutants and the accumulation of sludge in an NFT leads to a
definition of plant requirements and eventually to the potential of a solar
powered treatment system purifying waters. The hypothesized system would be
composed of three distinct sections:
1. Roughing or Preliminary Treatment;: Plant species with large root
systems capable of surviving and growing in a grossly polluted
condition. Large sludge accumulations, anaerobic conditions, and
trace metal precipitation and entrapment would characterize this
section. A large portion of the BOD5 and SS would be removed in
this section.
2

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NUTRIENT FILM SYSTEM
PLANT
GROW BLOCK
NUTRIENT SYSTEM
(continuous flow)
NOTRtENT
ioUJTlON
r
PURIFIED (WATE
ROOTS
CAPILLARY PAD
['.('iii'1 1.1. Tli^- mi t. rif-Mi I film Un-hn i qui- v;i r i !i I. i on of li.yd n >| nm i o plant product i .on systems.

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2.	Nutrient Conversion and Recovery: An active nutrient uptake, high
biomass and/or food production section would follow the first
section. The major interaction here would be nutrient conversions,
but suspended solids and trace organic removals would improve.
3.	Water Polishing: The third section would be a polishing section
that would necessarily have nutrient-limited plant production,
depending on the required effluent water quality.
A schematic of the three plant series wastewater treatment system, showing
the major pollution control functions and the by-products produced in each
section, is presented in Figure 1.2.
Obviously, the three modules in the NFT treatment system can also be used
separately for different levels of treatment of varying input water
quality. For example, the system may be applicable to polishing secondary
effluents or lagoon effluents. Testing to define the last section has the
potential of producing an effluent relatively free of trace organics and
nutrients. In essence, a modification of this section may result in a
viable alternative to the activated carbon adsorption unit process for water
supply treatment. It may also be used to improve the bacterial quality of
sewage effluents to avoid the chlorination step which is costly in terms of
operation and energy expenditures.
D. SIMILARITY WITH OTHER TREATMENT SYSTEMS AND ADAPTATION OF HYDROPONIC
SYSTEMS
The NFT principle differs in only one main aspect from typical hydroponic
systems in commercial use. The nutrient solution is applied as a thin film
flowing past the root. This feature provides nutrients and water supply to
the plant by capillary action in the root mass while maintaining root aera-
tion. At the minimal slopes, aeration limitations can result from pooling
of nutrient solution in the root mat, especially if the surface is not
smooth. The aeration problems can be solved by increasing either the slope
or flow rates. Aeration can be improved at higher flow rates, but the same
effects can be achieved with intermittent flow.
A simple comparison between the NFT and existing land application systems
can be made to clarify the differences between them. Known application
rates in land treatment systems and the flexibility of using various kinds
of plants would indicate that the NFT may be able to process wastewater at
higher rates, control and conserve nutrients and other pollutants as or
more efficiently, and provide control over and access for monitoring all
inputs and effluent constituents. Slow rate and overland flow land
treatment systems are limited to application rates of 5 to 16 cm (2 to 16
inches) per day, with application to soil once every five to seven days to
provide a resting period which enables oxygen to penetrate the soil surface
(among other effects created by the "resting phase"). The eventual design
of an NFT will most likely use daily application, with little or no resting
period, and applications of greater than or equal to 40 centimeters of water
per day.
4

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PLANT
PRODS.
PLANT PRODS.
PLANT PRODS.
,STABLE
SLUDGE 8
ROOT
MATERIAL
OUTPUTS
WASTE-
DISCHARGE
	^ OR
WATER REC.
WATER
SUSPENDED SOLIDS
AND ORGANIC
REMOVAL
NITROGEN AND	POLISHING OF
PHOSPHORUS AND (SOLUBLE ORGANICS
POTASSIUM REMOVAL (SUSPENDED SOLIDS,
' NUTRIENTS AND
FUNCTIONS;
SCREENINGS
PRELIMINARY TREATMENT
LAGOON PREAPPL1 CATION TRTMT.
OR
EQUALIZATION a
EMERGENCY STORAGE
PLANT SERIES I
PLANT SERIES H
PLANT SERIES CI
TOXIC ELEMENTS
Figure 1.2. Schematic di a.j/ram showing three-:':luge hypothesized Ni'T treatment syntem for
domestic wastewater with functions for each stage and fate of alJ material:.;

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Finally, the NFT appears to have interesting economic potential. If it is
assumed that the treatment area requirements will be less than 5 ha per
10,000 people, the capital cost of the structure will be between $2 million
and $4 million; a capital cost comparable to conventional treatment
systems. The production of valuable ornamental plants would result in an
annual gross return on the order of $1 million to $2 million per year. An
effort will be made to translate the system requirements into cost effec-
tiveness to clarify the above implications.
E. LIMITATIONS TO THE NFT WASTEWATER TREATMENT
While the NFT has shown promise in this pilot study, several comments must
be made regarding the practical limitations of the system. In order to
operate on a year-round basis the system nust be protected from low winter
temperatures by a greenhouse cover. The cover is also required to recover
evapotranspiration (ET) in summer, in addition to sufficient ventilation.
Attempting to grow valuable plants requires the retention of a qualified
grower as well as increased costs in terras of appropriate lighting and
environmental conditions. While this study used only a medium to low
strength domestic sewage, even this substrate may have contained phytotoxic
substances which in larger concentrations or shock loadings could severely
damage an NFT unit; for example, an ammonia spill once wiped out a small
stand of cattails. Another limitation is the possibility of overloading the
system with solids. While solids settle and are degraded in the root zone,
too heavy an accumulation can be damaging to some plants, as was seen in one
case with reed canary grass. One additional caution, rather than limita-
tion, is that plants must be selected for appropriate roles in the NFT
system; the characteristics and performance of a particular plant must be
matched to the role which it is to play.
Large scale testing is required to document the costs of qualified manpower
required to manage ornamentals and other plants that offer the possibility
of substantial revenue generation. These must be contrasted to the opera-
tion and maintenance costs of small secondary treatment systems such as
oxidation ditches or lagoons.
6

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CHAPTER II
CONCLUSIONS
1.	The nutrient film technique (NFT) has been shown to be a viable alter-
native for domestic sewage treatment in this three-year multidisci-
plinary effort. Pilot scale units up to 36 meters long have been
operated continuously with domestic sewage at flow rates of up to
11,000 1/d (3000 gpd) in New York and New Hampshire. Data presented
here should be considered conservative since most experiments were
conducted under "worst case" climate and temperature conditions. No
attempt was made to control the plant environments during most of the
testing.
2.	The goal of this study was to examine the NFT concept as a possible
wastewater/water supply treatment alternative. The results of this
study show that, for the first time, most terrestrial plants can be
considered as viable treatment organisms. The NFT process enables a
photosynthetically-powered wastewater treatment facility to be consid-
ered for most treatment alternatives.
3.	Application rates were examined which would enable the process to be
competitive with existing processes. After steady state conditions
were attained under a given loading rate, the loading rate was
immediately increased. The main species that was examined in these
tests was reed canary grass. Reed canary grass grown in the NFT system
from seed and from transplanted sod resulted in the production of
better than secondary effluent quality at an application of 10 cm/day
under all applications throughout the year. Application rates at 20
cm/day were found to result in the destruction of the reed canary
grass. Attempts to optimize multiple species systems that could exist
in low dissolved oxygen environments eliminated the 20 cm/d limita-
tion.
4.	After the system was defined an optimized unit was constructed and
tested on raw domestic sewage. This unit resulted in effluent quality
significantly better than secondary quality with applications of 30
cm/day.
5.	The area requirements and necessity for a greenhouse cover will vary
depending on the location of the system. The following estimates can
be made for an NFT secondary treatment system. The area needs appear
to be approximately 2.7 hectares (6.6 acres) for a wastewater flow from
a population of 10,000 people. This would be divided into two sec-
tions. The first section would be a roughing section of approximately
7

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one hectare. It would be subdivided into relatively small sections so
that the wastewater could be rapidly introduced, allowed to remain in a
quiescent state for approximately one hour, and then removed slowly so
that laminar flow conditions would occur. The second section would be
a polishing and nutrient removal section of about two hectares. The
capital investment of such a system appears to be attractive, and
energy requirements would be low since only low head pumping would be
required in such a system. Although a detailed economic analysis was
not completed in this study, it appears that the capital investment
would be less than conventional secondary treatment alternatives.
6.	The application of the NFT for nutrient removal is subject to the
limitations of all plant systems. Even with maximum growth rates the
nutrient removal rates are relatively low in comparison to high rate
nutrient manipulation processes such as microbial nitrification-
denitrification. However, partial nutrient polishing could be achieved
in NFT units of 10 ha/MGD or larger. These large greenhouse instal-
lations would be much larger than any other existing covered plant
installations. However, the value of plant products may be high enough
to justify use of the NFT as a nutrient recovery and/or water reclama-
tion unit operation.
7.	This study was able to define some of the general pollutant removal
mechanisms. By accumulating large quantities of biomass in the form of
fine roots, the possibility of removing pollutants is greatly
enhanced. The solids entrapped in the roots provide the largest
capability of this treatment system. Measurements of the solids within
the roots indicated that over 1,000 gm/m of entrapped solids was
accumulated. These solids represent significant potential for
manipulation of pollutant cycles. If, for example, this biological
organic material can be utilized for pollutant removal, then the solids
retention time becomes important. In such operations, reasonably high
loading rates result in solids retention times of greater than 100
days. This indicates that the process could be stable and provide an
efficient treatment system.
Organic removals are limited by the level of aeration. The capillary
mat of dense root systems may significantly increase aeration. The use
of plants such as cattails that translocate air to their roots could
also be a significant factor. Additional studies are needed in this
area.
The fine suspended solids were found to biocoagulate in the system, and
high clarity effluent was achieved under high loading rates.
8.	A major goal of this study was to manipulate the nitrogen cycle using
both plants and microbial interactions via nitrification-
denitrification. Nitrification was rarely observed during the study
and produced only 2 or 3 mg/£ of nitrate-nitrogen under the lowest
loading condition during the warmer time periods. This lack, of
8

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nitrification resulted in limited capability for control of the
nitrogen cycle with the NFT.
9. Phosphorous removal appeared to be limited by the plant nutrition
requirements. Due to the stress condition of most of the testing that
occurred, phosphorous removal rates were low in the NFT system.
10.	The relatively high temperature of domestic sewage is likely to provide
enough heat to maintain plant cultures in the NFT in most areas of the
U.S. where low cost solar greenhouses can be utilized. In some loca-
tions the greenhouse cover of the NFT system may be minimized due to
this available energy.
11.	The kinetics of pollutant removal were examined using several different
approaches. Due to the large number of variables, no comprehensive
model was established that would predict the process efficiency. The
following rates indicate the range of observed nutrient and organic
removals that were obtained in the system:
Removal Rate
Parameter	kg/ha-d
BOD	44-166
TOC	26-97
COD	99-247
SS	17-164
N	2.8-12.7
P	0.4-1.8
12. Both heavy metal and organic toxic materials were examined in this
system. Cadmium was added to the synthetic wastewater tested. Trace
organics were added to the domestic sewage in the New Hampshire test
facility. It is proposed that the removal mechanisms for the metals
and the organics was the extensive root surface area and the large
accumulated biomass. The following efficiencies were obtained for the
given organics tested:
Toxic Organics Tested	Removal Efficiency Achieved
Chloroform
96
Tetrachlorethylene
95
Benzene
94
Toluene
97
Trichloroethylene
91
Xylene
94
Bromoform
98
M-Nitrotoluene
96
Diethylphthalate
72
PCB 1248
94
9

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13. An attempt was made to define the relationship of hydraulic loading
rates and hydraulic retention time within the root zone. Due to the
large number of factors that affected the plants and the flow through
the system, only general comments can be made.
14. The most promising empirical approach provided by the kinetic analysis
of data indicated that the loading rate/effluent quality relationships
would hold over a limited range for domestic sewage. These relation-
ships will be of limited value in designing the NFT system for other
wastewaters.
15. This study attempted to conduct parallel testing with actual domestic
sewage and a synthetic sewage. Parallel comparison of these systems
indicated that it was possible to simulate the domestic sewage systems
with the synthetic wastewaters. Since the synthetic sewage contained
soluble substrates only, it was not possible to simulate solids
behavior or interactions with the synthetic sewage.
16. The water balance was established for a number of bench scale and pilot
tests. Although in many circumstances the expected loss of water
through evapotranspiration was equal to literature values of
approximately 50,000 2/ha-day, several test conditions resulted in
water losses up to 100,000 Jl/ha-day.
17. The energy and process requirements to recover evapotranspired water
from the greenhouse were examined. Energy costs of ET recovery are
extremely high and prohibit this alternative unless a low cost energy
source is available.
18. Although reed canary grass was examined in the majority of tests in
this study, a wide range of plants was cultured and propagated to
examine their viability in the NFT system when applied to wastewater
treatment. The following summarizes those plants that grew well and
those that were less acceptable under adverse conditions:
Plants that flourished
Cattails
Bulrush
Strawflowers
Japanese millet
Roses
Napier grass
Marigolds
Wheat
Phragmites
Plants with marginal growth
Bristly sedge
Chrysanthemums
Carnations
Tomatoes
Comfrey
Reed canary grass
Soft rush
Cucumbers
10

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19.	The above list of plants includes some that have high monetary value.
Reed canary grass, when grown under relatively low nutrient conditions,
resulted in biomass with a total nitrogen content of 5 percent with
most values greater than 4 percent. This indicates a total protein
content of greater than 30 percent of the total dry weight in many
cases and a plant that would have a significant animal food value.
Plants with ornamental value such as shrubs, trees, and roses would
represent plants with significant commercial value. Other plants that
showed promise in this study were several food plants that would be
useful for propagation purposes. Certain plants with a high value,
such as berry plants, could be cultured in this system. The potential
carry-over of toxic materials would be a concern with food products.
20.	The growth of plants in sewage, where the organic and suspended solids
loading rates are high, results in an optimum condition for inhibition
of plant growth and pest invasions. The application of hearty plants
such as cattails in the roughing sections of the NFT eliminated most of
the plant pathogen problems that were observed; however, fungus
invasions, insect attack on many of the plants, and other problems of
greenhouse plant production were common. All the typical greenhouse
production problems were controlled when advice from specialists was
sought and implemented. The destruction of a total treatment system by
pests or by contaminants in wastewater is a concern that needs careful
evaluation prior to full scale implementation.
21.	The technical and economic feasibility of the NFT as a treatment alter-
native was briefly examined in this study. It appears that the area
requirements to achieve secondary quality effluent from either raw or
primary settled domestic sewage would result in a cost competitive
system without taking advantage of any of the reduced operation costs
or the potential commercial benefits of biomass production. Solids
flushing and removal alternatives would be required with the applica-
tion of raw sewage. The estimates for tertiary treatment or water
reclamation with the NFT indicate area requirements that may not be
competitive with a conventional unit process unless the plant products
have a commercial value. If plant management techniques are achieved
that allow plant products to enter commercial markets, the use of the
NFT as a water reclamation facility shows significant promise.
22.	This study outlines a new approach to the use of plants as solar
powered water and wastewater treatment devices.
11

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CHAPTER III
RECOMMENDATIONS
This comprehensive feasibility study represents the first time that a wide
variety of plants has been shown to be possible candidates for use in waste
water and water supply treatment. The NFT process opens up a wide variety
of applications of photosynthetically powered treatment processes. The
results were intended to show the broad scope of applicability of plants to
waste treatment. The experiments, however, were subject to strong limita-
tions in terms of defining the fundamental interactions. In the complex
plant solution systems it was difficult to understand how pollutants were
removed and what happened to them in many parts of the NFT process. The
following outlines topics that need further attention preceding the commer-
cialization of the technology.
A.	ADDITIONAL SPECIES SCREENING AND PARAMETRIC EVALUATION
While this work has identified many plant species that could grow well in
NFT systems, the study did not match pollutant uptake characteristics with
plant end uses. The interaction of the many variables in pollution control
with potentially valuable plants will require a considerable screening
effort to identify rapidly growing plants with useful products that can
safely enter commercial markets. Considerable research needs to be done on
this topic. In addition, experiments need to be conducted over the life
cycle of plants to measure their useful life span in an NFT system.
B.	POLLUTANT REMOVAL MECHANISMS
This study delineates the loading rates and some of the associated retention
times that are required to remove pollutants in the NFT system. The primary
missing knowledge is the location and fate of many of the pollutants. For
example, the system has a potentially large capacity to entrap and use
microbial solids in root systems since they accumulate over 1000 gm/m and
the solids retention time is greater than 100 days. The effectiveness of
this microbial mass in pollutant control and methods of manipulating it must
be determined. The impact of surface area and bacterial attachment in the
NFT also remains to be defined. It is likely that the photosynthetically
produced surface area is one of the most important parameters controlling
pollutant removal. Also whether bacteria associate with or grow on these
surfaces as attached films and their capability for pollutant control needs
to be defined. Some form of solids removal must be incorporated so that
rapid flushing can be used to remove bioflocculated solids on an intermit-
tent basis.
12

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C. AERATION DUE TO CAPILLARY MAT IN REED-TYPE PLANTS
There is some information that suggests that the oxygen supplied by the
capillary mat in the NFT in hollow plants, such as the bulrushes, is a
significant factor in solids control. Biocoagulation of fine suspended
solids in this study was observed under most conditions. This resulted in
the production of a highly clarified effluent. The factors that control
this may contribute to a useful method for solids control.
D.	LOWER NUTRIENT EFFECTS ON YIELD
Before the NFT can be applied as a nutrient removal system, methods of
defining plant performance with limited nutrients must be known. Since
plant production under limited nutrient conditions is not the goal of most
agricultural operations, relatively little information exists. There are,
however, certain management approaches that would enable nutrients to be
removed from the wastewater while limiting growth substances are applied
directly to the plant leaves. Management techniques to provide a high
quality effluent from the NFT system should be examined.
E.	ET RECOVERY
The large energy and capital investments for evapotranspiration recovery in
greenhouses is a strong negative factor in the production of ultrapure water
from the NFT system. However, some large measured ET values, exceeding by
many times the literature values reported for normal plant production,
suggest that the capillary mat in the NFT can be managed to increase the
water recovery from NFT systems.
F.	DRINKING WATER AND POLISHING APPLICATIONS
Numerous water supplies are contaminated with nitrates and other nutrients
that would provide a nutrient base for an NFT system. Plants should be
tested for drinking water and other polishing-type applications. This
research should include examination of organic exudates that are produced in
the roots and other by-products that could be observed in clean water
applications.
G.	NITROGEN CYCLE MANIPULATION WITH THE NFT
One of the most important aspects of wastewater treatment is control of the
nitrogen cycle. The lack of nitrification in the long-term testing in this
project was one of the surprising outcomes. Methods of achieving
nitrification with the NFT should be examined.
H.	SCALE-UP OF OPTIMUM NFT SYSTEMS
The positive nature of this study indicates that the technology may play a
wide role in pollution control. Efforts should be made to test the NFT at
larger scale under longer term operations. Until example facilities are
available for review by the pollution control engineers, this innovative
technology is likely to be largely Ignored.
13

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CHAPTER IV
BACKGROUND AND LITERATURE REVIEW
The use of planes in pollution control systems is an old concept, but few
definitive studies have been conducted to identify and quantify the
mechanisms responsible for pollutant removal. Even less information is
available for the application of the unique hydroponic plant growth system
referred to as the nutrient film technique (NFT) to sewage treatment. To
obtain background information on the new application of the NFT it is
necessary to review a wide range of literature and topics.
More than 10,000 plant and soil waste treatment systems are presently in use
in central facilities, and more than 60 million people depend on aspects of
the plant-soil complex to purify septic tank effluents (Jewell and Seabrook,
1979). Total land area requirements for conventional aquatic or land
treatment systems range from several hectares to over 100 ha to process
sewage generated from 10,000 people (see Table 4.1). In order to be a
viable alternative, the NFT systems must offer better pollution control
and/or cost advantages. Clearly, this is a formidable challenge since the
NFT system will be a more complex plant system requiring a greenhouse cover
in northern climates. In order to make such systems cost effective it will
be necessary to reduce the area requirements and/or increase the value of
the plant by-products.
Background literature was drawn from seven distinct subject areas:
1.	commercial crop production in traditional hydroponic (soilless culture)
systems;
2.	development of the Nutrient Film Technique (NFT) as a commercial
alternative for growers including:
•NFT hardware development,
•Plant culturing and nutrient experience with various crops in NFT, and
•NFT spin-offs such as vertical growth tubes and adaptations for root
crops;
3.	biological habitat and life-cycle studies of emergent aquatic plants;
4.	aquaculture for waste treatment, including natural and man-made marshes,
wetlands, ponds, and other systems;
lU

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TABLE 4.1. STATE OF THE ART CHARACTERISTICS OF AQUATIC SYSTEMS AND LAND APPLICATION SYSTEMS USED
FOR DOMESTIC SEWAGE TREATMENT (from Reed, Bastian and Jewell, 1981 and EPA 1981)
Effluent	Pre-	Area Required
Kind of	Quality	treatment ha/10,000 people or
Treatment System Achieved	Required ha/3785 m^ flow-d	Comments
I. Aquatic Systems




A. Wetlands
(Artificial
or Natural)
Secondary+
Primary
10 to 30
Significant potential:
economics and design
need clarification.
B. Aquatic Plant
Systems
Secondary
Polishing
Secondary
Primary
Secondary
7+
2.5
Most information
limited to water
hyacinths, a warm
climate nuisance plant.

Upgrading
Secondary to
Tertiary
Secondary
5.4

II. Land Applications




A. Slow Rate
Drinking water
None
23 to 280
Hundreds of systems
In use.
B. Overland Flow
Secondary +
Lagoons
6.5 to 44
Recent design criteria
make them a useful
alternative.
C. Rapid
Infiltration
Tertiary
None to
Secondary
3 to 23
Often limited due to
availability of soils.

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5.	land application of wastewater by overland flow or slow-rate irrigation
methods;
6.	wastewater and water treatment in traditional hydroponic systems; and
7.	conventional unit process wastewater treatment.
The NFT is a relatively new technology which has gained widespread
acceptance by commercial plant growers only in recent years. The basic NFT
configuration depends on the wastewater characteristics and the type of
environment in which plants would be expected to function. Treatability
studies in conventional systems yield parameters such as retention time
needed for desired levels of treatment. Plant selection is accomplished in
part by matching environmental tolerances of various plants with the
conditions assumed or known to exist in an NFT wastewater treatment system.
Such data have been compiled for many species, including wetland plants.
The hypothesized NFT system assumes a "unit process" type of system in which
well-adapted wetland species will occupy the initial units. These would be
loaded with relatively large applications and act as biological filters and
settling units, providing efficient roughing treatment to secondary quality
levels. This effluent should provide substrate for growth of high value
crops in lower rate NFT units of the type investigated to date in this
research. In this way, there should be minimum compromise necessary between
plant value and hardiness under adverse conditions. The polishing phase of
this two-part system should be able to take advantage of the NFT's reported
ability to serve as a medium for growth of almost any plant species
(Schippers, 1977, 1979).
This review will concentrate on development of the NFT, plant culturing
experience in the NFT, and wastewater treatment using any hydroponic
system. The broader areas of hydroponics development, aquatic plants,
aquaculture, land application of wastewater, and conventional wastewater
treatment will be treated only as they relate specifically to NFT wastewater
treatment. Aquatic plant physiology and morphology are discussed in some
detail to illustrate the potential applications of the NFT system.
A. HYDROPONICS
Development of the general field of soilless plant culture systems, of which
NFT is an outgrowth, goes back many years and encompasses many variations.
No general review of hydroponics literature is attempted here. Hyppio
(1974) provides a short, selected list of publications on hydroponics which
includes entries from 1939 onward. Sholto-Douglas (1976) describes "nutri-
ent flow" techniques bearing much similarity to NFT which have been in use
in India since 1947. In the original version, plants were placed in holes
in bamboo stems, through which nutrient solution flowed. Later, hoses and
polyethylene sheeting were used. The Hygro-flo tube system, reported by
LeSleur and Brungard (1977), is a process developed in the U.S.
independently of NFT; but it shares some of the attributes of NFT.
16

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The nutrient film cultural method was first developed as a research tech-
nique in 1966, but it was Alan J. Cooper who first recognized the commer-
cial potential of the principle. Cooper's original research on the commer-
cialization of the Nutrient Film Technique (NFT) was conducted at the
Glasshouse Crops Research Institute (GCRI), Littlehampton, Sussex (Cooper,
1976). It was first introduced to the public by the group in 1969-70.
Cooper was involved in early work with General Electric on the concepts
discussed in this report. Additional university research has been conducted
in the United States by Vinzant (1978) and Schippers (1979). Large-scale
commercial development of the system was accomplished by the General
Electric Company in Syracuse, New York. Many firms are active in the supply
of equipment for NFT crop production, both in this country and in Europe.
Cooper (1976) provided a booklet of reprints of his original articles in The
Grower, a weekly English journal for the horticultural industry. The first
of these articles appeared in May, 1973, in which growth experiments with
tomatoes were conducted. No large-scale NFT trials had been attempted at
that time.
The NFT principle differs in only one main aspect from typical hydroponic
systems in commercial use. The nutrient solution is applied in a thin film
of liquid that flows through and over the root mass. This feature allows
for nutrient and water supply to the plant by capillary action in the root
mass while maintaining good root aeration. Plastic tubing or gullies
(hydrocanals) make a desirable, impermeable, and chemically non-reactive
surface for containing the gravity-fed nutrient solution. Because of the
hydrophobic nature of most plastics, a capillary mat may be used to disperse
the solution across the width of the gully. Rounded bottom gullies have
been used but tend both to limit root growth and to result in more pooling
of solution. Raised sidewalls and tops have been added to the gullies to
retard evaporative loss and to provide some mechanical support. In turn, a
high humidity level is maintained around the roots. The top surface of the
gullies are variously black to prevent light transmission and white or
aluminum to prevent nutrient temperature buildup. This plant production
concept is now popularized and can be purchased from several seed and hard-
ware companies for small home applications. This home scale application
emphasizes the ease with which plants can be produced in the NFT system.
Schippers (1977a and 1977b) traced NFT development in an annotated biblio-
graphy which provided a concise summary of design changes, commercial appli-
cations, advantages and problems associated with the NFT, and the formula-
tion and testing of nutrient solutions. Major advantages cited are the ease
of plant handling, lack of water loss due to drainage, elimination of soil
salinity and disease problems, quick visual inspection of the system and the
whole plant, and energy savings due to heating of nutrient solution, result-
ing in lower greenhouse heat demand. Recommendations on nutrient formula-
tions at first were very specific for each crop. However, experience showed
that standard formulations could be used equally well on a variety of
crops. This is a strong point in support of the use of NFT for wastewater
treatment. Perhaps more incentive to apply the technology to wastewater
treatment arose when plants were shown to thrive in low concentration
nutrient solutions, contrary to what was believed originally.
17

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Lauder (1976) demonstrated the use of timber-framed, plastic-lined gullies
0.91 meter (one yard) wide, for NFT production of grasses at GCRI. This
basic design was adapted for use in this project, without the absorbent
liners used in the GCRI units.
Potential problems discussed in Schippers' review Include pooling of nutri-
ents in uneven gully floors, deleterious effects of high ammonia nitrogen
concentrations (limits not defined), "collar burn"—a condition arising from
crystallization of salts, and perhaps most troublesome, root death.
Darkening and withering of roots are mentioned in several articles, and are
attributed to various causes, including oxygen deficiency and microbial
infestation, particularly fungal blights. See Chapter VI for examples of
root death in some Cornell test species.
One major difference in NFT development between Great Britain and the U.S.
is that the English growers are encouraged to buy whole systems from commer-
cial suppliers, while in this country the emphasis is on their building the
systems, following guidance from the researchers. Schippers (1977) provides
a manual for growers on constructing and operating NFT systems. This infor-
mation is updated in Schippers (1979) which presents a historical review and
summary of three years' experience at the Long Island Horticultural Research
Laboratory with various crops.
B. WASTEWATER AND WATER TREATMENT IN HYDROPONIC SYSTEMS
The authors are aware of only one other group studying the application of
the NFT to wastewater treatment. The Waste Recycling Program of the
Plymouth Portsmouth Polytechnic has been conducting a broad scope waste
recovery research program at a large sewage facility in England since mid-
1977 (Stead 1977, Stead 1978, Stead and Rome 1979, Anonymous 1982). One
component of this study was the application of reverse osmosis for recovery
of high quality water. The concentrated permeate from this unit process was
used as a feed solution to the NFT. Comprehensive experiments showed the
potential for production of a wide variety of food crops. Figure 4.1 shows
some of the facilities in use by the Plymouth research group. The main
focus of this activity has been on cultural aspects and the production of
useful plant matter. These studies are continuing.
A few studies have been reported which involve hydroponics in some form of
wastewater treatment or effluent polishing. Four separate computer litera-
ture searches (Including the Aqualine system at the Water Research Center,
Stevenage, England) were conducted. Of the twenty-two possible studies
dealing with aquaculture, aquatic plants or hydroponics in sewage or waste-
water treatment, the NFT was not mentioned.
Lewis et^ al. (1978) illustrated the use of hydroponic tank-grown tomatoes as
a nutrient scrubbing unit of a recirculating-water system for fish produc-
tion. The purpose of the study was to grow fish and tomatoes rather than
treating wastewater; thus the authors' primary concern was to maintain a low
ammonia concentration and provide a method to remove nitrate from the
system. Channel catfish were grown in three 870-liter tanks with mechanical
18

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Plymouth, England

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aeration and automatic dally feeding. Effluent from the tanks passed
through biofliters (rotating biological contactors, or RBC's) for treatment
of organics and conversion of nitrogen and phosphorus to nitrates and phos-
phates. These were assimilated by tomato plants as clarified RBC effluent
passed through the^hydroponic system. The system worked well in preventing
NH3, NO3- and POi+~ buildup in the fish culture tanks, and tomato yields
were approximately twice that of field trials on the same varieties.
Removals of NC>3~-N and POi+~ -P averaged 14.3 and 14.8 percent, respectively,
on a concentration basis, not adjusted for evapotransplratlon losses.
Considering that nitrate concentrations were usually fairly low (6 to 10
mg/£), the authors concluded that this system showed promise of being able
to meet nitrogen standards for fish culture when used in a single pass
(treat and discharge) mode. Ammonia nitrogen did not exceed 1 mg/£. Such a
system without biofliters was reported by Sneed et al. (1975), who examined
the production of several plants in catfish holding tank effluent.
Chinese water chestnut was grown on floating vermiculite rafts in catfish
tanks in South Carolina by Loyacano and Grosvenor (1973). Three treatments
of pools stocked with catfish were replicated in their study: open pools,
pools with rafts only, and pools with water chestnuts growing in the rafts.
Both the raft and water chestnut treatments resulted in Increased fish sur-
vival and more efficient feed conversion. Water chestnut yields amounted to
a rate of 3176 kg/ha of pool surface over a seven-month period. No water
quality data were given, but this study also demonstrated the feasibility of
hydroponic plant production on this type of wastewater. Harmon (1980) con-
ducted tests to determine whether effluent water produced from an intensive
fish farming operation could be treated with the NFT. The results of three
small scale experiments were positive although inconclusive.
Sias and Neven (1973) described a hydroponic gardening system in which
beans, radishes, and tomatoes were grown In secondary sewage effluent from
the City of Melbourne, Florida. A major emphasis was on comparison of
irradiated versus chlorinated effluents (>_ 2 ppm free chlorine residual),
and plant growth was also compared In sewage versus nutrient solutions.
They generally found much better growth in irradiated or untreated waste-
water effluent than in chlorinated effluent or in chlorinated, irradiated,
or untreated nutrient solutions. Nitrate, ammonium and phosphate levels
were monitored in two hydroponic units, one planted with beans and radishes
and the other without plants. Results were not reported, however, because
the small size of the units did not provide enough plants to obtain measur-
able uptakes. The units consisted of containers of perllte, 15 cm (six
inches) deep and 107 cm (42 inches) in diameter. Fifty-seven liters (15
gallons) of sewage or nutrient solution were used every three days, which
translates to a loading of only two centimeters (0.8 inches) per day over
the available tank surface area. Wastewater characteristics were not
addressed except for some discussion of coliform counts in the various
experiments.
Wastewater hydroponics formed one part of an overall study of nutrient
recycling in hydroponic and aquacultural systems at UCLA (Gordon, 1977;
Wallace et al., 1978). Hydroponics was considered only in conjunction with
conventional wastewater treatment. Severe Iron (Fe) deficiency occurred in
20

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early tests even when sewage was renewed twice daily. In later greenhouse
studies, Fe supplements were given In the form of Insoluble iron applica-
tions to the roots before transplanting to the tanks, or Fe chelate added
directly to the sewage effluent. Conclusions drawn to date are that the
system provided for commercial-scale production of cucumbers, tomatoes,
lettuce, and chrysanthemums, with significant heavy metal reductions and
variable N and P reductions in the wastewater.
The solar aquacell process (Serfling and Alsten, 1979) has been demonstrated
in at least three California communities in pilot scale since 1976. The
process has been the subject of many articles in various journals and pro-
ceedings since its inception in 1972 as an Intensive aquaculture system for
raising freshwater fish and shrimp. It was noted in working with this
system that water quality was maintained at a high level even though the
fish waste and the commercial fish feed, cow manure, and chicken manure used
as nutrient sources produced an organic and nutrient loading very similar to
that of domestic sewage. Of the major wastewater constituents, only phos-
phates were significantly lower in the fish production system. Results from
two California communities, Solana (5700 I/day) and Cardiff (11,400-22,700
£/day), Indicated that primary effluent averaging about 90 mg BOD5/A and 170
mg BOD5M, respectively, could be treated to BOD5 levels of about 10 mg/l or
less in four days' retention time in the aquacells. At the same time, sus-
pended solids were reduced from about 100 mg/l to well below 10 mg/l. Good
nitrification was achieved, but effluent nitrate levels would not meet
drinking water standards. Phosphate levels were highly variable. Estimates
based on limited data showed an average removal rate of 0.5-1.0 mg/l total
phosphate per day.
It should be pointed out that the higher plant component of the solar aqua-
cell process consists of floating plants (duckweed and water hyacinth) which
contribute little direct benefit to wastewater treatment. The plants
provide shade to limit algal growth, some uptake of nutrients, heavy metals
and other toxic substances, and provide filtration and microbial attachment
sites in their roots. Water hyacinth and duckweed have conferred these
benefits on wastewater lagoon systems in other studies (for example,
Wolverton and McDonald, 1979). The aquacell system employs inert plastic
mesh webbing strips anchored to the pond floor to provide additional surface
for microbial attachment. In addition, the ponds are aerated and contained
within a double-wall polyethylene greenhouse. These last three items
provide the bulk of the aquacell's treatment performance improvement over
conventional lagoons. Also, the aquacells are always used in conjunction
with some form of primary treatment, either settling basins or anaerobic
lagoons. The authors state that secondary treatment levels can be achieved
within two days' HRT without floating plants in the system, according to
their operational experience. So, although the system contains some
elements of hydroponics, It is not a true hydroponic treatment system as
described elsewhere in this report. It is rather a combination of lagoon
and conventional treatment technology, designed to provide shorter retention
times and more consistent treatment in situations where lagoons would be
appropriate. Hydraulic retention times on the order of days do not compare
favorably with HRT demonstrated for the NFT system in this study. The
largest solar aquacell system to date, 1300 m /d (0.343 MGD), in Hercules,
21

-------
California, has been delayed In achieving full operation due to an under-
sized primary treatment unit (Parkinson, 1981).
C. PLANT PHYSIOLOGY AND MORPHOLOGY IMPLICATIONS
Effective and economical sewage treatment in the NFT system is influenced by
plant survival, nutrient uptake, growth rates, and yield. Although a large
amount of information exists on aquatic plant growth, key questions remain
to be addressed: what differences exist between nutrient solution and
sewage behavior in the NFT; what are the physiological implications of the
differences to plant survival and growth; and finally, which plants are best
able to thrive in the sewage environment. In this chapter literature
pertinent to these questions is reviewed.
As the NFT system must remove several fractions of wastewater contaminants
(dissolved and particulate organlcs and inorganics), plant characteristics
other than survival are important. Particulates are removed as the water
velocity slows; since the density of planting, root mass, and shoot diameter
affect the water velocity. Because dissolved inorganics can be removed by
bacterial and plant uptake, the plant growth rate, blomass production and
the root surface area available for bacterial attachment are important
factors. Table 4.2 summarizes the plant characteristics critical for
successful operation of some removal mechanisms in NFT sewage treatment.
This chapter also reviews the occurrence of plant characteristics among
taxonomlc groupings and the influence on these characteristics of growth in
sewage film. Similarly, Table 6.17 (Chapter VI) shows those characteristics
considered desirable in an NFT system as a whole.
TABLE 4.2. PLANT CHARACTERISTICS IMPORTANT FOR WASTEWATER TREATMENT
MECHANISMS IN AN NET SYSTEM

Type
Pollutant Removed



Dissolved
Dissolved
Plant Characteristic
Particulate
Inorganic
Organic
Blomass
+ *
+
0
Growth Rate
0
+
0
Density of planting
+
+
+
Shoot diameter
+
0
+
Root/shoot ratio
+
+
+
Root surface area
+
+
+
Root volume
+
+
0
Root exudation
0
•
—
*(+ =» positive effects; 0 ¦ no effect; - = negative effect)
22

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C.l Redox Potential and the Effect of Sewage Film on the Plant Root
Environment
The nutrient film technique for hydroponic plant culture is successful
because the trickle of solution supplies plant roots with nutrients and
maintains an adequate exchange of dissolved gases. When sewage is used as
the nutrient film, the nutrient supply and gas exchange may be depressed due
to direct and indirect effects of the reduced state of chemicals in the
sewage film. It is likely that depressed dissolved oxygen concentrations
will have the greatest effect on plant species that can survive and grow in
the NFT system.
The reduced chemicals act as a sink for dissolved oxygen in the sewage film
from both chemical oxidation and biochemical utilization of the reduced
substrates. Microbial utilization of the substrates will raise the concen-
tration of carbon dioxide in the sewage film. In addition, the bacterial
and fungal populations developing on the plant root masses will create a
physical barrier to gas exchange between the roots and the sewage film.
As oxygen is depleted in the sewage film, the oxidation state, solubility,
and consequent availability of mineral nutrients will change. Redox poten-
tial, a measure of electrochemical potential or electron availability, may
be used to indicate the degree of oxidation or reduction in the root zone.
The influence of redox potential on mineral nutrient availability and plant
growth has been reviewed by Gambrell and Patrick (1978) and Ponnamperuma
(1972) for submerged soils. Although the sewage film is quite different
from soil and soil water in levels of minerals and exchange sites, chemical
behavior in both systems can be expected to be similar. The chemical trans-
formations that affect nutrient availability and toxin production can be
predicted from the redox potential of the system.
Figure 4.2 summarizes the approximate critical threshold redox potential
values for some nutrients important to plant growth. When molecular oxygen
is present in a solution, the redox potential usually will range from +400
to +700 mV (Gambrell and Patrick, 1978). As oxygen is depleted by chemical
and microbiological activity, nitrate becomes reduced. Nitrate can be used
by many organisms as an electron acceptor; the redox potential will
stabilize between +200 and +400 mV until nitrate is depleted.
Manganic compounds (Mn+lf) reduce to the more soluble manganous (Mn+^) at an
oxidation level comparable to that of nitrate (200 mV). There are, however,
fewer organisms which are ab'le to use manganic compounds directly as
electron acceptors.
The next system to be reduced is the ferric-ferrous compounds. Iron is
particularly important to plants. Fe(OH)3 will be reduced to the highly
soluble Fe+ at around +120 mV. Ponnamperuma (1972) considers that this
reduction is probably not biologically mediated and therefore not important
in flooded soils. However, Bohn et al. (1979) stated that iron redox
reactions occur at appreciable rates in soils without enzymatic catalysis,
and thus iron reduction may occur.
23

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FIGURE 4.2. APPROXIMATE CRITICAL THRESHOLDS FOR STABILITY OF REDOX SYSTEMS
Redox
Potential
mV
+400 ,
+300
+200
+100
-100
-200
-300
Electrochemical Reaction
O2 + 4H+ + 4e"
- 2H2O
Oxygen disappears
NO 3" + H 2O + 2e"
MnO 2 + 4H+ + 2e"
Fe(OH)3 + e"
Fe(OH)3 + e~
~	N0 2~ + 20H~	Nitrates Reduced
~	Mn+ + 2H 2P	Manganese Reduced
-	Fe+2 + 30H"
**	FE(OH) 2 + OH- Iron Reduced
CH 3COCOOH + 2H+ + 2e"
CH3CHO + 2H+ + 2e"
CH 3CHOHCOOH
CH 3CH 2QH
Organic Fermentation
SOi+= + H2O + 2e~
SO 3= + 3H20 + 6e~
SO 3" + 20H"
S= + 60H~
Sulfates Reduced
CO 2 + 2H+ + 2e"
CO 2 + 8H+ + 8e~
HCOOH
CHit + 2H^)
Methane Fermentation
-400
2k

-------
Intermediate dissimilation products of organic matter (fermentation) form
the next oxidation-reduction couples. Pyruvate-lactic acid is one example
cited in Stumm and Morgan (1970), who point out that organic carbon com-
pounds (with the exception of methane) are unstable over the entire range of
redox potential, but anaerobic conditions are more favorable to their
preservation than aerobic conditions.
Sulfate reduction will be initiated when the redox potential falls to
approximately -150 mV. Hydrogen sulfide (H2S) results, and may escape from
the sewage film as a gas. Ponnamperuma (1972) reports that proteins, fatty
acids, amines, and diamines will be degraded at this redox level as well.
Methane fermentation can occur as the redox potential falls between -250 and
-300 mV. Gambrell and Patrick (1978) conclude that most of the sulfate must
be reduced to sulfide before methane production begins. However, most of
the other redox systems can overlap; for example, nitrate may be reduced in
the presence of oxygen, and manganese may be reduced in the presence of
nitrate but not in the presence of oxygen.
In addition to the changes in availability of plant nutrients, a reduced
root zone has important implications for the presence of phytotoxins. The
end products and by-products of anaerobic metabolism by root zone organisms
can be toxic to plants. Jackson (1980) considers incompletely oxidized
carbon compounds produced from anaerobic metabolism to be phytotoxins.
Examples of such compounds are ethanol, acetaldehyde, and the aliphatic
acids: acetic, butyric, and propionic acid.
The sewage film thus induces conditions of low dissolved oxygen, heightened
carbon dioxide concentrations, changes in nutrient availability, and pres-
ence of phytotoxic substances relative to aerobic soils and non-carbonaceous
nutrient film solutions.
C.2 Physiological Implications of Growth in Sewage Film
C.2.a Plant Response to Anaerobiosis—
The effects on the whole plant of an anaerobic or reduced root zone have
been documented by plant physiologists interested in the impacts of flood-
ing. Much of this literature has been reviewed by Drew and Lynch (1980);
they summarize the general effects of anaerobiosis as: slowing of leaf and
shoot extension, chlorosis, epinasty and abscission of lower leaves, wilt,
stunting and degeneration of roots, formation of adventitious roots at the
base of the stem, and inhibition of transport of essential nutrients, min-
erals, etc., from the root to the shoot. Research has focused on the cause
of the plant symptoms (oxygen depletion, carbon dioxide accumulation, or
phytotoxins) and the mechanism of induction of the response (hormonal
disruption, nutrient deficiencies, or low energy from glycolysis).
Early studies demonstrated that the rate of mineral accumulation in roots is
directly related to the oxygen deficiency (Steward and Berry, 1936). A
series of experiments by Hopkins et al. (1950) tested the effect of three
25

-------
concentrations of dissolved oxygen (0.2, 0.9 and 2.2 mg/1) on root growth
and whole plant response of tobacco, soybeans, and tomatoes. At the lowest
concentration of dissolved oxygen, root extension stopped; at 0.9 and 2.2
mg/1 adventitious roots were stimulated. The growth and color (intensity of
green) of the whole plants were proportional to the oxygen concentration in
the solution. Most of the responses of the plant were proportional to the
log partial pressure of oxygen in the nutrient solution. Elemental
composition of the plants in these experiments indicated that amounts of
many major nutrients (potassium, calcium, magnesium, and phosphorus) closely
paralleled growth response.
The effects of low oxygen on the morphology and size of roots was tested by
Geisler (1965), who grew peas in aerated and non-aerated hydroponic cul-
ture. Roots in the non-aerated system (dissolved oxygen 3-4 mg/I) showed
less growth and fewer primary and secondary lateral roots. The lateral
roots were more closely spaced, and the amount of branching increased in the
non-aerated system. A conflicting opinion was voiced by deWit (1978) who
concluded that roots in non-aerated culture will be more porous, straighter,
thicker, and more numerous, that root hair growth will be suppressed, and
that the absorption surface will be larger.
Work by Chang and Loomis (1945) tested the effects of carbon dioxide on
mineral and water absorption by plants grown hydroponically. Solution
vessels were purged of oxygen by carbon dioxide or nitrogen gas, and the
absorption of water, nitrogen, phosphorus, calcium, magnesium, and potassium
was measured. Absorption of all five minerals and of water were reduced by
the CO2 treatment, and not by N2. The implication was that the effects of
the CO2 treatment were due to the gas and not to the exclusion of oxygen.
The reduction in Ca and Mg absorption was proportional to the reduction of
water uptake, while the relative absorption of N and P were half that of
water. K was released from roots in two of four experiments. The
implication of these results is that Ca and Mg accumulation is least
dependent on metabolic activities, that K metabolism is very sensitive to
the root environment, and that N and P require an active transport.
The role of CO 2 as an indicator of flooding damage was confirmed by Kramer
and Jackson (1955) in a study of tobacco plants. Wilting could be induced
by displacing O2 with CO2 in soil column experiments; the wilting response
was much more severe with CO 2 than with N2. A review of these and other
experimets concluded that a partial pressure of CO 2 (pC0 2) of 5 to 10% in
the culture solution would inhibit nutrient and water uptake and retard
growth of roots and shoots (Ponnamperuma, 197 2).
Attempts have been made to separate the effects of low oxygen, elevated CO2
and phytotoxins on the plant response to anaerobiosis in the root zone.
Sanderson and Armstrong (1980) tested the separate effects of anoxia, vola-
tile fatty acids, and ethanol on conifer roots, and concluded that anoxia
was the primary cause of root death. Trought and Drew (1980) conducted
experiments to monitor and correlate external changes in the root zone and
internal changes in the whole plant. The rapid changes observed in the
whole plant (decrease in accumulation of shoot dry matter, inhibition of
root growth, decrease in transpiration rate) were apparently in response to
26

-------
a rapid decline in O2 concentration. CO2 and ethylene accumulated much more
slowly.
Despite some experimental evidence that CO 2 levels are critical, the current
consensus seems to be that lack of oxygen is the primary factor in water-
logging damage to plants. Experiments that tested the minimal partial
pressure of dissolved oxygen that still allowed root growth and function are
equivocal, due to the difficulty in actually measuring the oxygen pressure
experienced by the root. Certain experiments indicate that dissolved oxygen
tensions as low as 0.1 mg/1 at the porous cortex may be sufficient for ion
uptake (Drew and Lynch, 1980).
Hormonal control in the plant is strongly implicated as the mechanism res-
ponsible for the visible symptoms of damage from low dissolved oxygen in the
root zone (see Russell, 1977, for a review of plant hormones). Poor aera-
tion in the root zone inhibits the synthesis and translocation of cytokinin
and giberellins. These plant hormones inhibit the symptoms of premature
senescence (chlorosis and abscission) that are provoked by an internal
redistribution of the mobile nutrients (N,P,K) to younger leaves. Reduction
in leaf extension and shoot elongation has also been associated with inter-
ference in the synthesis and transport of cytokinins and giberellins.
Adventitious rooting, the emergence of porous roots from the base of the
stem, has been correlated with inhibition of giberellin synthesis and
transport in the low oxygen root environment (Reid and Crozier, 1971).
Accumulation of abscisic acid in the leaves of flooded plants may contribute
to stomatal closure, which would account for the reported decreases in
transpiration rate (Drew and Sisworo, 1979). This plant hormone is also
associated with early leaf senescence and inhibition of shoot growth.
Ethylene levels rise in the aerial portions and in the roots of flooded
plants. Chlorosis of leaves has been associated with increases of ethylene
in plant shoots. Kawase (1981) has determined that ethylene trapped in root
cells will induce cellulase activity, which causes cell death and disinte-
gration. Lacunae (open channels in the root cortex) are formed when the
cells die. This response may be adaptive for the flooded plant, as lacunae
have been implicated as passageways for oxygen flux from the shoots to the
roots. Lacunae are discussed in greater detail in section C.3.a.
The changes in plant hormone levels are due to the failure of roots to pro-
vide nutrients; the failure of the roots to provide nutrient ions is due to
lack of oxygen (Drew and Sisworo, 1979). Thus, although hormonal disruption
provokes the symptoms of waterlogging damage, the mechanism must be traced
back to the lack of oxygen.
A deficiency of oxygen will force cells to metabolize anaerobically.
Metabolism of carbohydrates without oxygen as a final electron acceptor
(fermentation, glycolysis) in the root cells of flooded plants has important
implications: the yield of energy to the cell is reduced, and toxic end
products or by-products of fermentation may accumulate. Vartapetian (1978)
considers the low energy yield to be of fundamental importance in the dis-
ruption of nutrient uptake and assimilation. Ethanol, an end product of
27

-------
glycolysis, kills cells by solubilizing lipids, inactivating mitochondrial
enzymes, and causing cell leakage (Crawford, 1978).
The physiological effects of the reduced or anaerobic root zone are sum-
marized in Figure 4.3. Other indirect effects should be mentioned as well.
For example, plants may not exude water in the absence of light (e.g., at
night) (guttation) when under water stress. Drew and Lynch (1980) review
the relationship of waterlogging and plant pathogens and note that damaged
roots may favor invasion by pathogens. The indirect effects of nutritional
status may also influence plant susceptibility to invasion. Resistance
mechanisms active in the healthy plant (for example, microrhizal associa-
tions and certain enzymatic pathways) may be impaired in the damaged plant.
C.2.b Exogenous Phytotoxins—
While the major components of sewage (biodegradable organics and solids) may
be predicted from the size of a community, trace contaminants may vary
widely. Table 4.3 shows the content of a number of metals in influent
streams of municipal sewage. The concentrations of metals can vary over
several orders of magnitude depending on the industrial sources. This
section discusses the roles of metals and other trace level contaminants as
potential plant toxins.
Plants require certain elements in their metabolism (Table 4.4).
Non-essential elements are assimilated as well and are of concern regarding
possible phytotoxicity and potential removal from the waste stream.
Bryan (1976) described the uptake of heavy metals by aquatic plants as a
passive process, which can be indirectly influenced by metabolism. The
kinetics of zinc uptake involve a rapid adsorption of Zn onto the cell
membrane, followed by diffusion through the membrane and binding to proteins
within the cell. The effect of zinc metabolism is seen in the amounts of
protein available as binding sites.
Much of the research on plant responses to heavy metals has been conducted
to assay the agronomic impacts of sewage sludge application. While this
research is not directly applicable to the NFT process, it gives some
indication of the role of metals as toxins. Cadmium and lead have been
particularly well studied, due to their toxicity to humans. This research
can provide general guidelines to relative rates of uptake and phytotoxicity
of heavy metals to plants. However, most of the data are on crop plants
grown in soil (Cunningham et al., 1975 and Chaney et_ al., 1975). The pH and
organic matter content of soil exert a strong influence on the availability
of metals to plants (John et al., 1972); consequently, the data from soil
culture experiments are not readily transferable to hydroponic culture.
Hydroponic culture experiments have been conducted to assay the accumulation
of certain heavy metals. Koeppe (1977) measured the uptake of lead and
cadmium from hydroponic culture using crop plants. Uptake of both metals
was considerable and varied with nutrient regime. After three days growth
in 100 mg/1 Pb, corn roots had accumulated 10,600 m Pb/g dry weight and
corn shoots had accumulated 390 Pg Pb/g dry weight. Cd uptake was similar,
28

-------
f\3
Dlsuolved Gases
Diffuse Slowly {
loductiou of
CcLl til one
Ethylene
Trapped
Root Cclle
Metabolite
Anacroblcally
Disruption Id
Nutrient Uptake
d Aaoimllatlon
Nutrient
Availability
Changes With
Redox Potential
^.Oxygen Depletion
from Respiration
Root Cell Death
Disruption in
Root Hormone
Synthesis and
Translocation
}
low Growth of
Roots and
Shooto*
•Visible Symptom
Figure U-3- Summary of physiological effects of the flooded root tone.
Aerenehyna
Formation*
Increased Erudation
Adventitious	of OrganicB From
ootlo* 		Rool°
tiaulated*
Partial Closure
of Stoma
Dccieased
^Transpiration Rate
Seneucence or
(Wilt*
Inhibition ol
Guttation*
.Redistribution
of Mobile Nutrlenti
(H.P, K) Within
Shoote
Older Leaves*

-------
TABLE 4.3. POTENTIALLY TOXIC ELEMENTS FOUND IN RESIDENTIAL
TRUNK SEWERS (From Davis and Jackson, 197 5)
Element
Concentration (mg/ £)
Cd
0.007
- 0.019
Cr
0.008
- 0.09
Cu
0.12
- 0.21
Pb
0.075
- 0.12
Ni
0.014
- 0.09
Zn
0.20
- 0.25
TABLE 4.4. ESSENTIAL MINERALS
FOR HIGHER PLANTS (From Keeton, 1980)
Element
Approx. no.
one hectare
of kilograms needed to grow
of corn (of average yield)
MACRONUTRIENTS


Nitrogen (N)

180
Phosphorus (P)

45
Potassium (K)

140
Sulfur (S)

85
Magnesium (Mg)

55
Calcium (Ca)

55
Iron (Fe)

2
MICRONUTRIENTS


Manganese (Mn)

0.3
Boron (B)

0.07
Chlorine (Cl)

0.07
Zinc (Zn)

Trace
Copper (Cu)

Trace
Molybdenum (Mo)

Trace
30

-------
but this metal was more readily translocated and the large gradient between
the roots and shoots was not observed. Cd concentration of leaves of plants
(crop and vegetable) grown In solution culture were reported by Page e£ al.
(1972) (Table 4.5). The accumulation of Cd was proportional to the concen-
tration of the metal in solution; leafy vegetables accumulated more of the
metal in the edible portions.
TABLE 4.5. AMOUNTS OF CADMIUM ABSORBED BY VARIOUS PLANT SPECIES IN RELATION
TO SOLUTION CADMIUM CONCENTRATION (From Page et al., 1972)
Solution	Plant species
concen-
tration
Corn
Turnip
Beet
Bean
Tomato
Cabbage
Lettuce
Pepper
Barley
Ug/m*


Cadmium Concentration
of Leaves, ug Cd/g

Control*
0.29
0.32
0.30
0.12
0.11
0.75
0.14
0.98
0.10
0.1
90
160
280
9
58
—
—
—
—
0.25
234
270
321
27
115
—
—
—
—
0.50
165
394
295
34
138
—¦
—
—
—
1.0
227
469
326
35
174
212
384
104
75
2.5
—
—
419
—
334
420
430
180
86
5.0
—
—
755
—
591
714
588
314
110
10.0


1078

1122
822
751
578
175



Total
Cadmium Accumulated In
Leaves,
Ug

Control*
5.3
5.0
1.7
3.3
1.1
5.3
1.1
2.1
0.95
0.1
1262
1944
784
200
—
—
—
—
—
0.25
2111
1544
764
205
—
—
—
—
—
0.50
1713
1387
384
157
—
—
—
—
—
1.0
2184
1960
430
112
1183
1297
1219
133
437
2.5
—
—
302
—
1712
2142
1268
158
432
5.0
—
—
680
—
2438
3798
1058
314
522
10.0


323

3310
2754
788
474
525
*The concentration of Cd in the control solution was 0.0005 ug/m£.
Field data have been used to assay the uptake of heavy metals by aquatic
plants. Mayes et al. (1977) in a study of a contaminated lake, concluded
that Elodea canadensis can assimilate nonessential trace metals from sur-
rounding water and sediments. Accumulation of metals by aquatic plants
grown In an environment polluted by fly ash is reported by Guthrie and
Cherry (1979) and is summarized in Table 4.6. Again, the aquatic plants are
shown to accumulate metals.
31

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TABLE 4.6. CONCENTRATION OF FLY ASH POLLUTANTS ABSORBED BY AQUATIC
PLANTS. (From Guthrie and Cherry, 1979)
Element

Annual mean
concentration,
wet weight (ppm)
Sediment
Water
Duckweed (Lemna
sp.) Cattail (Typha sp.)
Al-Si
40,656
13
3,741
370
Fe
20,812
17
1,110
141
K
8,149
6.1
1,705
2,775
Mg
5,461
4.1
625
602
Ti
2,388
0.9
108
21
Ca
1,833
9.2
789
2,070
Na
687
7.7
289
65
Ba
294
0.7
34
67
Mn
96
0.07
80
425
Sn
84
0.1
18
7.0
CI
84
3.8
213
847
V
63
0.04
5.1
0.6
Cu
51
0.4
8.1
5.4
Cr
38
0.2
5.2
1.3
As
19
0.06
5.8
0.4
Co
10
0.06
1.5
0.2
Zn
6.4
0.4
4.6
2.8
Se
6.1
0.1
1.7
3.9
I
4.6
0.08
1.2
0.4
Cd
1.7
0.1
1.4
0.4
Br
1.2
0.1
2.8
15
Sb
0.9
0.07
0.8
0.2
Hg
0.8
0.04
0.5
0.2
How Che high levels of metals reported in these studies affect the plants
is of concern. Foyet_al. (1978) reviewed the physiological effects of
metal toxicity in plants and pointed out that each metal causing
phytotoxicity does not have a characteristic symptom. General symptoms are
stunting and chlorosis. Chlorosis may appear to be an iron deficiency and
can be induced by excesses of zinc, copper, nickel, and cadmium. Cd
interferes with photosythesis of terrestrial plants, partly by reducing the
concentration of photosynthetic pigments and altering their ratio; no data
are available for aquatic plants (Alabaster and Lloyd, 1980).
Studies of the phytotoxicity of metals in nutrient solution culture have
been few. Page et al. (1972) report Cd levels inducing a 50% reduction in
growth of test plants (Table 4.7). Note that the sensitivity of the test
plants varies over an order of magnitude. Table 4.8 summarizes several
studies of phytotoxicity in floating or submergent aquatic plants. In
general, the aquatic plants tested were most sensitive to copper and arsenic
levels in the solution culture. These two metals have been commonly used as
aquatic herbicides; their concentrations in domestic sewage are below
32

-------
damaging concentrations, but the concentrations that inhibit plant growth
could be a problem in many wastes.
TABLE 4.7. SOLUTION Cd CONCENTRATIONS PRODUCING 50% GROWTH REDUCTION,
VISUAL SYMPTOMS ASSOCIATED WITH 50% GROWTH REDUCTION, AND
CONCENTRATION IN LEAF AT 50% GROWTH REDUCTION
(From Page et al., 1972)
Solution	Concentration	Visual Symptoms
Cd Concentration	of Cd in Leaf	Associated With
Plant Range Producing 50%	at 50% Growth	50% Growth
Tested Tested Growth Depression Depression	Depression
	 Ug Cd/m£ -
Cabbage	0-10	9.0
Tomato	0-10	4.8
Barley	0-10	5.6
Pepper	0-10	2.0
Sweet corn 0-1.0	1.2
Lettuce	0-10	0.9
Red beets	0-10	0.2
Field beans	0-1.0	0.2
Turnips	0-1.0	0.2
Ug Cd/g*
800	t
570	Chlorosis
120	t
160	Chlorosis
230	Reddish-orange
coloration of
leaf margin,
necrosis, and
chlorosis
320	Chlorosis
290	Wilting
22	Wilting
290	Chlorosis
*Oven dry weight, 70°C.
tNo specific symptoms were associated with the reduced growth.
The literature on phytotoxicity of non-metallic substances to aquatic plants
is limited. Table 4.9 summarizes the studies found in a brief survey. Oils
are capable of spreading over large areas of air/water interface and plant
surfaces and interfering with gaseous exchange.
In summary, comparison of Tables 4.3 through 4.8 indicates that aquatic
plants should tolerate the levels of heavy metals found in typical municipal
waste streams. Copper, cadmium, and arsenic levels may be of most concern,
as aquatic plants are extremely sensitive to these metals.
C.2.c Plant Growth In Sewage Compared to Natural Habitats—
Seldel (1976) found that if certain plant species were grown in polluted
water instead of in a natural habitat, changes in morphology, anatomy, and
physiology of those species could result. For example, Allsma plantago-
aquatlca, which normally has a small storage bulb, grew to gigantic size in
sewage and formed a very large storage bulb which was replaced by a new one
33

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TABLE 4.8. EFFECT OF HEAVY METALS IN THE NUTRIENT SOLUTION ON GROWTH OF
FLOATING OR SUBMERGENT AQUATIC PLANTS (page 1 of 2)

Test Concen-



Metal
tration (mg/ X.)
Plant
Effect
Reference*
Copper
0.25
Myriophyllum spicatum
50% reduction in root weight
1

0.15
Elodea canadensis
50% reduction in photosynthesis
2

0.46
Elodea canadensis
90% reduction in photosynthesis
2

3.1
Elodea canadensis
50% damage to whole plant
2

0.13
Lemna minor
50% damage to whole plant
2
Arsenic
2.9
Myriophyllum spicatum
50% reduction in root weight
1

0.85
Elodea canadensis
50% reduction in photosynthesis
2

2.6
Elodea canadensis
90% reduction in photosythesis
2

0.90
Elodea canadensis
50% damage to whole plant
2

0.15
Lemna minor
50% damage to whole plant
2

11.5-143
Mixed aquatic plants
Net production of microcosm
3


(microcosm)
decrease to zero

Cadmium
7.4
Myriophyllum spicatum
50% reduction in root weight
1

0.01
Lemna valvidiana
25% reduction in growth of
4



whole plant


0.05
Lemna valvidiana
80% reduction in growth of
4



whole plant


12.8
Elodea canadensis
50% reduction in photosythesis
2

23.5
Elodea canadensis
90% reduction in photosythesis
2

148
Elodea canadensis
50% damage to whole plant
2

14.8
Lemna minor
50% damage to whole plant
2

10.10-89.6
Potamagoton crispus
No reduction in biomass or
5



photosynthesis

Chromium
1.9
Myriophyllum spicatum
50% reduction in root weight
1

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TABLE 4.8. Continued (page 2 of 2).
Metal
Test Concen-
tration (mg/fc)
Plant

Effect
Reference*
Mercury
3.4
Myriophyllum splcatum
50%
reduction
in root weight
1

0.80
Elodea canadensis
50%
reduction
in photosynthesis
2

1.69
Elodea canadensis
90%
reduction
in photosynthesis
2

7.4
Elodea canadensis
50%
damage to
whole plant
2

1.0
Lemna minor
50%
damage to
whole plant
2
Aluminum
2.5
Myriophyllum splcatum
50%
reduction
In root weight
1
Zinc
21.6
Myriophyllum splcatum
50%
reduction
In root weight
1

8.1
Elodea canadensis
50%
reduction
in photosynthesis
2

23.1
Elodea canadensis
90%
reduction
in photosynthesis
2

22.5
Elodea canadensis
50%
damage to
whole plant
2

67.7
Lemna minor
50%
damage to
whole plant
2
Silver
0.10
Elodea canadensis
50%
reduction
in photosynthesis
2

0.18
Elodea canadensis
90%
reduction
in photosythesis
2

7.5
Elodea canadensis
50%
damage to
whole plant
2

0.27
Lemna minor
50%
damage to
whole plant
2
Thallium
1.43
Elodea canadensis
50%
reduction
in photosythesis
2

2.75
Elodea canadensis
90%
reduction
In photosythesis
2

2.0
Elodea canadensis
50%
damage to
whole plant
2

0.008
Lemna minor
50%
damage to
whole plant
2
Lead
363
Myriophyllum splcatum
50%
reduction
In root weight
1
~References for Table 4.8: 1. Stanley, 1974; 2. Brown and Rattlgan, 1979; 3. Giddlngs and
Eddlemon, 1978; 4. Alabaster and Lloyd, 1980; 5. Mcintosh et al., 1978

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TABLE 4.9. POTENTIAL PHYTOTOXICITY OF HYDROCARBONS AND OTHER ORGANIC COMPOUNDS.
Substance
Concentration
Plant
Effect
Reference*
Toluene
10 mg/£
Whole oils 100-10,000 mg/A
Chloramlnes
Phenol
6-25 pg/A
100 mg/fc
Macrocystls angustlfolla
Mixed algae cultures
Mixed aquatic plants
(microcosms)
Elchhornla crasslpes
75% reduction in
photosynthesis
Inhibition of photo-
synthesis and growth
No changes in photo-
synthesis and
respiration
No evidence of toxicity
(Plants could remove 36
mg phenol/g dry weight)
~References for Table 4.9:	1.	Environmental Protection Agency, 1976;
2.	Vandermelen and Ahem, 1976;
3.	Larson et al., 1978;
4.	Wolverton and McKown, 1976.

-------
each year. Scirpus lacustris normally sheds its roots each year, but when
grown in certain types of sewage, it did not. When cultured in unsettled
sewage the plant did not produce roots; a rhizome, losing its scale-like
leaves, took over mineral absorption. When a bed of this species was
floated in sewage, there was no change in stem characteristics. In
unsettled effluent from a fabric printing factory, this species produced ten
to twenty times more biomass than when grown in uncontaminated water.
Seidel also found in this study that when S. lacustris was grown in an
oxygen-poor medium, the aerenchyma expanded greatly; however, fiber content
remained unchanged. Other anatomical variations observed were a change from
uniform to irregular stomatal form and arrangement, and the formation of
hydropods (water cells) on the epidermis when the plants were cultured in
phenol-containing medium rather than in uncontaminated water.
C.3 Plant Adaptation to Sewage
C.3.a Mechanisms of Adaptation—
Despite the limitations to plant growth imposed by a polluted anaerobic
environment, a number of plants can grow and thrive in environments of
reduced oxygen. These kinds of plants would be ideal for "roughing treat-
ment" in the initial stages of a NFT. Aquatic and wetland plants such as
cattails, rice, water lilies, and mangroves are common examples. These
plants possess a combination of morphological and biochemical adaptations to
avoid or tolerate the conditions induced by the anoxic environment. These
adaptations and examples of plants showing these characteristics are shown
in Table 4.10.
Early investigations into mechanisms of resistance to the anaerobic environ-
ment were made by naturalists, who observed the morphology of wetland and
aquatic plants and inferred function from structure. Two structures,
lacunae (or aerenchyma) and adventitious roots, have been widely cited as
adaptations to the reduced root zone.
Lacunae are longitudinal canals in the root cortex that extend into the
plant shoot. The tissue in which the lacunae occur is called aerenchyma.
Laing (1940) described lacunae in aquatic macrophytes. Oxygen diffusion
from the aerial portions of the plant through the lacunae and to the roots
was postulated as the mechanism of resistance to anaerobiosis, as anaerobic
metabolism and the accumulation of phytotoxic end products could be
avoided. However, Crawford and Tyler (1969) argued that lacunae could be an
adaptation to minimize the amount of respiratory tissue per volume of root,
and consequently reduce the overall demand for oxygen in the plant.
Indirect evidence that oxygen can be transported from the aerial portions of
plants to the roots was provided by Armstrong (1967), who demonstrated
oxygen diffusion from the roots of bog plants to the rhizosphere when grown
in anaerobic soils. Oxygen loss from roots has other important implica-
tions. If a microzone around the roots is oxidized, the plant roots will
avoid contact with reduced phytotoxic substances (e.g., metals, sulfides,
37

-------
and organic acids which are generally rendered Insoluble in an oxidized
environment) in the soil. Note that solubility is a function of not only
the oxidation-reduction state but also pH and competing liquid
concentrations.
TABLE 4.10. PLANT ADAPTATIONS TO SURVIVE AN ANOXIC ENVIRONMENT.
Adaptation
Examples of Plants Exhibiting Adaptation
Morphological
A. Lacunae to transport
oxygen from shoot to
root
Nuphar luteum (water lily)
Spartina alterniflora (marsh grass)
B. Adventitious roots to
facilitate ethanol
diffusion
C. Storage tubers to over-
come energy limitations
of glycolysis
Zea mays (corn),
Helianthus annuus (sunflower),
Triticum sp. (wheat),
Oryza sativa (rice)
Saglttarla latifolla (arrowhead)
Biochemical
A.
Alternative fermentative
pathways to minimize
ethanol accumulation
Senecio aquaticus (ragwort, groundsel)
Veronica pereglna (speedwell)
B. Nitrate reductase to
use nitrate as an
electron acceptor
Lemna minor (duckweed)
Rumex acetosa (green sorrel)
Mentha aquatica (mint)
Armstrong suggests a physiological classification of plants based on their
oxidizing capabilities drawn in part on the work of Conway (1937) and Stolzy
et al. (1962). The series consists of: (1) those which can tolerate very
strongly reducing conditions because of considerable root oxidizing power
and internal oxygen supply (e.g., Menyanthes trlfoliata and Oryza sativa;
(2)	those whose oxidizing capabilities are less (e.g., Molinia coerulea);
(3)	those which, although able to receive internal respirating oxygen, are
unable to oxidize even mildly reduced soil; (4) partially soil-oxygen
dependent plants; and finally, (5) those mesophytes with high soil-oxygen
requirements (Stolzy et al., 1961).
Direct measurement of gas flux through the lacunae of Nuphar lutea was
recently reported by Dacey (1980). Dacey measured oxygen movement at rates
up to 50 cm/minute through the lacunae, and concluded that gas movement was
by mass flux, not by diffusion.
38

-------
The second frequently ciced adaptation to the anaerobic root zone is the
presence of adventitious roots. Jackson (1955) determined that plants with
adventitious roots showed fewer symptoms of stress and a faster recovery
when flooded with low oxygen water. The response of the plants was indepen-
dent of depth of flooding. Adventitious roots facilitate the diffusion of
ethanol, a toxic end product of glycolysis, into the rhizosphere; Crawford
(1978) considers this to be the mechanism of resistance.
Root porosity (the fraction of the root filled with gas) is higher in adven-
titious roots than in primary roots (Yu et al., 1969). Consequently,
increasing the number of adventitious roots will increase the overall root
porosity of a plant. Adventitious rooting was induced in sunflowers, wheat,
and corn when the plants were flooded (Yu et al., 1969); therefore, plants
not normally grown in flooded conditions were shown to survive by increasing
oxygen and ethanol diffusion through the new, porous roots.
The adaptations described thus far allow plants to grow in the anaerobic
environment by avoiding glycolysis (by oxygen transport from the shoots) or
by eliminating the toxic end product of glycolysis (by ethanol diffusion
through adventitious roots). Crawford (1978) suggests that the tubers of
aquatic plants are a third morphological adaptation to anaerobic condi-
tions. The large supply of carbohydrates present in the tubers would help
compensate for the inefficiency of energy production via anaerobic metabo-
lism until the shoots emerge above the sediment surface.
Another adaptation to the anaerobic environment is the use of biochemical
pathways that do not form ethanol. Plants resistant to anaerobiosis mini-
mize the accumulation of ethanol by using alternative fermentative pathways
and by minimizing the conversion of acetaldehyde to ethanol (Crawford and
Tyler, 1969; Crawford, 1978).
The alternative fermentative pathway forms malate, an organic acid, from
glucose via phosphoenolpyruvate (PEP) carboxylase (Crawford and Tyler,
1969). The organic acid is less toxic than ethanol. The conversion of
acetaldehyde to ethanol is catalyzed by an enzyme, alcohol dehydrogenase
(ADH). Levels of ADH were measured in a series of experiments on plants
resistant and non-resistant to anaerobiosis; the root cells of non-resistant
plants showed a high level of ADH when the roots were flooded, while resis-
tant plants showed no increase in the enzyme (Crawford and Tyler, 1969).
Crawford and Tyler (1969) further proposed that malate may accumulate in
flood-resistant roots because of the absence of malic enzyme. However,
Davies et al. (1974) found that malic enzyme was present in a large number
of resistant and non-resistant plants. Crawford later suggested that malic
enzyme activity in resistant plants is inhibited when the roots experience
low oxygen concentrations (Crawford, 1978).
Several objections have been raised to the metabolic theory of flooding
tolerance. Drew and Lynch (1980) point out that a significant amount of
ethanol is produced in all plants during glycolysis, and that resistance may
reflect the ability to eliminate or tolerate ethanol itself. Smith and
ap Rees (1979) reported the results of enzymatic assays to clarify the
39

-------
pathways of carbohydrate fermentation in resistant plants. They concluded
that the catalytic activities of ADH, PEP carboxylase and malic enzyme were
all appreciable in the resistant plant roots; when grown anaerobically, ADH
levels increased but PEP carboxylase was not detectable. In experiments
with a labelled substrate (sucrose), the end products of fermentation were
ethanol and lactate, with minimal malate levels. Clearly, futher experi-
ments are required to reconcile these different results.
Use of an alternative electron acceptor in the absence of oxygen is yet
another adaptation to life in an anaerobic root zone. Garcia-Nova and
Crawford (1973) suggest that nitrate may be used in this manner, citing as
evidence the increased level of nitrate reductase activity in flooded roots
of resistent plants.
C.3.b Indicators of Adaptation to Anaerobic Conditions—
Plants clearly differ in ability to tolerate anaerobic conditions in the
root zone. Knowing what properties, if any, resistant plants have in common
would simplify the search for plants able to thrive under the proposed NFT
system. One obvious indicator of tolerance is the plant environment;
resistant plants are those which grow in anaerobic environments. However,
experiments in which crop plants were flooded indicated that plants normally
grown in aerated soil showed varying degrees of flooding tolerance.
Plant species are attractive for use in the NFT system for reasons other
than survival (for example, removal of nutrients or particulates) or
production of a valuable crop. An indicator of resistance to anaerobiosis
in the root zone would help screen candidate plants chosen for additional
qualities.
The research into metabolic pathways of carbohydrate fermentation discussed
in the previous section suggests several possible biochemical indicators of
tolerance to anaerobiosis. Crawford and Tyler (1969) noted that alcohol
dehydrogenase (ADH) induction upon flooding was greater in non-tolerant
plants than tolerant plants. This response has been suggested as an
indicator (Crawford, 1978). Chirkova (1978) noted a direct relationship
between the affinity of ADH for its substrates, ethanol and acetaldehyde,
and flooding tolerance.
Garcia-Novo and Crawford (1973) suggest that the activity of nitrate-
reductase in roots may be a good index of flooding tolerance. The enzyme
level was increased in all their test species, but increased to highest
levels in the flooding tolerant species.
Bartlett (1961) noted that the accumulation of iron oxides on plant roots is
correlated with the ability of plant roots to oxidize a small zone of the
rhizosphere. Plants that assimilated iron into the aerial parts of the
plants were not as resistant to flooding.
Another indicator of resistance to anaerobic root zones has been proposed by
Armstrong (1978), who measured the ability of roots of several plants,
UO

-------
mainly forage crops, Co grow into reduced (paddy) soils. He found Chat root
penetration was highly correlated with the plant's ability to oxidize a dye,
o-naphthylamine.
Use of a dye to indicate ability to transport oxygen into roots has been
proposed by Philipson and Coults (1978). Indigo-carmine dye is suggested;
the dye is colorless when reduced and blue when oxidized. A blue halo will
appear around the root of a plant capable of oxygen transport.
Although the number of proposed indicators suggests that rapid screening of
plants for tolerance characteristics is quite feasible, a note of caution is
warranted. Tolerance to flooding appears to vary greatly between species
and even varieties of the same species. Linhart and Baker (1973) tested the
physiological response to flooding of different individuals of a species of
a weed (Veronica peregina L.) growing in an area that was flooded each year
from December through March. Plants raised from parents that germinated
under water in the center of the pond and grew for several weeks on flooded
soil accumulated malate when flooded. Those plants raised from parents
collected from the edge of the pond did not accumulate malate. Clearly,
habitat remains an important indicator of tolerance to anaerobiosis.
C.3.c Identification of Plants Tolerant To NFT Sewage Environment—
Using the descriptions of suitable plant characteristics, it is possible to
identify plants that may be grown in an NFT system using sewage. A compila-
tion of more suitable plants is shown in Table 4.11. This table also
includes a description of plants' growth forms and distributions.
C.4 Environmental Factors Affecting Tolerant Plants
C.4.a Effect of Water Level on Growth of Emergents—
Emergent aquatic plants, by definition, have shoots growing above and roots
growing below a body of water. The natural habitat of emergent aquatic
plants, however, ranges from shallow water and continually saturated soils
to periodically inundated (tidal) areas, to seasonally dry lowlands, and
meadows. Fluctuations in water levels and consequent changes in aeration of
the rooting zone are thus conditions to which this group are specifically
adapted. For this reason, the emergents are ideal for use in wastewater
treatment in NFT systems where the root mass is partly exposed to the air
and partly surrounded by an anaerobic medium.
Nutrient availability is greatly enhanced in anaerobic substrates where the
reduced form of ionic species occurs. Uptake of toxic levels of many sub-
stances by emergent plants in this reduced zone is avoided by diffusion of
oxygen and oxidizing compounds from the roots into the substrate. Nutrients
and potential plant toxins are precipitated and adsorbed to particulate
matter in this oxidized zone of the actively growing root tip; the oxidized
zone decreases in size toward the basal portions of the root, where presum-
ably uptake occurs. Fluctuating water levels would appear to have a similar
bl

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TABLE 4.11. EMERGENT SPECIES TOLERANT OR POTENTIALLY TOLERANT TO NFT SEWAGE ENVIRONMENT
(From Fassett, 1957; Muenscher, 1944; and Cook et al., 1974) (page 1 of 7).
Species
Growth Form
Distribution
Notes
1. Cattail Family
(Typhaceae)
Typha latlfolla
(Common cattail)
Typha augustlfolla
(Narrow leaved
cattail)
Stout, upright plants up
to 6 ft (2 m) tall, form-
ing dense colonies. Spreads
mostly by creeping rhizomes.
Flowers in May/June.
Monoecious, perennial.
Hybrids of these two
species where they are
found growing together.
Cosmopolitan;
Newfoundland to
Alaska, south
throughout most
of U.S. and into
Mexico
Pistillate spike often
gathered for arrangements;
persists until winter.
Hairy down of spike is
used for packing material.
Leaves are resistant to
decay and absorb water and
swell. They are harvested
extensively in the Fall
and stacked for drying.
Uses include caulking,
packing nursery stock,
weaving. Rootstocks are
rich in starch.
2. Grass Family
(Graminac)
Phragmltes austrails
(Reed, Reed grass)
Stems are stout, upright;
reach 16 ft (5 m); from
coarse, scaly rhizomes;
sometimes with long stems
creeping extensively over
moist soil surface. Peren-
nial; persistent throughout
fall and winter. Flowers
in August/September.
Widespread; through-
out S. Canada and the
U.S., except In S.E.
U.S. In freshwater
and brackish marshes,
around springs, and
along shores of lakes
and streams.

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TABLE 4.11. Continued (page 2 of 7).
Species
Growth Form
2. Grass Family (cont.)
Spartina pectinata
(Cordgrassl
S. alterniflora
TSaItmarsh cordgrass)
S. patens
TSaltmeadow cord-
grass )
Greet stems, 1-2 m tall;
from 8tout, scaly, cord-
like rhizomes. Leaves long
and tough. Two forms of S.
alterniflora: a short form
(0.3-0.4 m) on high marsh;
a tall form (1.2-2.0 m) on
intertidal zone. Optimum
pH 4.7-7.8. Perennial;
scaly rhizomes that may
comprise the bulk of total
plant biomass (depending on
age of stand and season).
Phalaris arundinacea
(Reed canary grass)
Slender grass up to 2 m tall
generally in dense colonies.
Erect stem. Perennial.
Distribution
Notes
S. pectinata found in
shallow, sandy lakes
and freshwater marshes,
also in brackish marshes
along Atlantic coast.
Widespread except in
south. S. alterniflora
found in salt marshes
along seacoast from
Maine to Texas.
S. patens in salt
marshes along Atlantic
coast; rare in inland
marshes in Hew York
and Michigan.
Extensive salt meadows
of patens along the
Atlantic coast are
sometimes cut for
'salt hay.' Formerly
used extensively for
packing and bedding
material.
Newfoundland to
Alaska, south to
North Carolina,
Missouri, and
California. Grows
in areas subject
to inundation.
Planted as a forage
grass on marshy
meadows in many areas.

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TABLE 4.11. Continued (page 3 of 7).
Species
Growth Form
2. Grass Family (cont.)
Zizania aquatica
(Wild rice, of East)
Z. aquatica var.
auguat ifolia
(Northern wild rice)
Z. aquatica var.
interior
(Large wild rice,
of midwest)
Stout, erect stem; 2-3 m
long. Many 'prop' roots
emerge from lower nodes.
Roots easily pulled up. Leaf
blades flat, 1-5 cm wide.
Inflorescence 30-50 cm long,
monoecious; annual. Var.
augustifolia 0.5-1.5 m tall;
leaves 4-12 mm wide. Var.
interior 0.9-3 m tall; leaves
1-4 cm wide. The latter two
varieties intergrade where
their ranges overlap, but
ordinarily are distinct.
Seeds do not withstand
drying; for purposes of
propagation it must be stored
moist (in water or wet moss)
at 1-3°C until planting.
Zizaniopsis miliacea
(Southern wild rice)
Perennials with creeping
rhizomes, culms 1-3 ft high,
Leaf blades 1-2 cm wide;
panicles 30-120 cm long.
Calamagrostis
canadensis
(Bluejoint)
Tall, slender grasses, from
extensively creeping root-
stocks, making swales.
Inflorescence is open in
C. canadensis.
Distribution
Notes
Z. aquatica found in
Ijrackish water along
coast; South Maine to
Florida; inland to North
Indiana and E. Wisconsin.
Var. augustifolia, South
New Brunswick to N. Maine,
west to N. Minnesota and
N. Indiana. Var. inter-
ior, Indiana, and South
Minnesota to Nebraska
and Texas. All forms
require a soft, fertile
bottom; inland, this group
is found in shallow water,
lakes, slow streams, and
edges of marshes.
Seeds from natural
stands are gathered
for human consumption
and the plant is
grown commercially.
It is introduced to
many areas as food
for waterfowl. See
Chambliss (1940).
In North and South America;
semi-aquatic to aquatic;
mostly in marshes, creeks,
and river banks.
C. canadensis found in
moist places; Greenland
to Alaska; south to
Maryland, North Carolina,
Missouri, Kansas, Colorado,
Arizona, and California.

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TABLE 4.11. Continued (page 4 of 7).
Species
Growth Form
Distribution
Notes
2. Grass Family (cont.)
-p-
Arundo donax
(Reed, Giant reed)
Arundinaria tecta
(small cane)
Robust; stem 2-6 ra, many-
noded; hollow. Leaf blades
up to 70 mm wide and up to
1 m long; inflorescence
dense, erect, 30-60 cm long.
Rhizome thick, creeping,
knotty.
Introduced to the U.S.
from warm regions of
the Old World; occurs
along rivers, irrigation
ditches, 8treams; on
river banks, occasion-
ally in marshes.
Arborescent or shrubby,
simple or branching stems,
1 to 5 ra tall. Flowers in
May/July. Perennial.
Lacunae in rhizomes reported
by McClure (1963) (not in A.
macrosperma). Also called
switch-cane, scutch-cane, reed.
From Maryland to Indiana
and Missouri, Florida,
and Texas; in swamps
and moist soil. So-
called "cane-brake."
The culms, or flower-
ing stems, are used
for lattices, mats,
and screens in the
construction of adobe
huts; they are impor-
tant as sources of
musical reeds (for
clarinets, organ
pipes, etc.) and for
industrial cellulose.
Both species of
Arundinaria, commonly
known as bamboo, are
used for construction,
furniture and decora-
tive purposes; the
young shoots are also
edible.
Arundinaria
macrosperma
(Giant cane,
also called
A. gigantea)
Possibly intergrading
species with A. tecta;
this plant is the
largest member of the
grass family,
colonies in low, moist
From southern Ohio,
Illinois and Missouri,
south to Florida and
Texas. Extensive
colonies in low, moist
woodlands and along
streams and rivers.

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TABLE 4.11. Continued (page 5 of 7).
Species
Growth Form
3. Sedge Family
(cyperaceae)
Carex spp.
C.	aquatilis
C.	comosa
C.	lanuginosa
C.	inflata
C.	vesicaria
C.	crinita
C.	lurida
C.	lupulina
C.	hystricina
C.	pseudo-cyperus
A large and complex genus,
differentiated by the nature
of the perigynium (sac
enclosing the nutlet).
The most common species
are listed, and may be
described as follows:
grasslike perennials; rhizo-
matous or tufted; stems solid
usually leafy and trigonous;
up to 2 m high. Inflorescence
varies from a much branched
panicle to a simple spike.
Cladium jamaicensis
(Saw grass)
C. mariscoides
"TTwig rush)
Plant is 1-3 m high; leaves
broad (5-10 mm wide) with
serrate-spiny margin and
lower midrib.
C. mariscoides smaller
To. 3-1 m high, leaves narrow
(1-3 mm wide), not serrate-
spiny.
Distribution
Notes
About 1,000 species
world-wide; cosmopoli-
tan, mainly temperate.
Most species occur
in damp places (wet
meadows, swales);
a few are aquatic.
C. jamaicensis is found
chiefly on the Atlantic
coastal plain (Virginia
to Florida) and along
the Gulf coast to Texas.
It is the dominant plant
in large areas of the
Florida Everglades.
C. maris coides is a
northeastern species,
also found along the
Great Lakes. Both
species grow in fresh or
brackish waters in swamps,
marshes, and shallow water.
C. jamaicensis has
been considered as
a source of fibers
for paper-making,
and C. mariscoides
is used extensively
for thatching.

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TABLE 4.11. Continued (page 6 of 7).
Species
Growth Form
Distribution
Notes
3. Sedge Family (cont)
£ze
erus s
PP-
C.	papyrus
C.	rotundu's
C.	articulTtus
C.	ochraceus
C. rotundus perennial ;
numerous slender rhizomes
with small tubers; stems
20-50 cm, smooth; leaves
3-6 mm wide, equal in length
to stems.
Widespread in tropics,
north to Virginia and
occasionally adventive
further north.
Scirpus acutus
(Hardstem bulrush,
Big bulrush)
Circular stem, 3-10 mm thick, Widespread in U.S.,
S. americanus
s
(Chair-maker
rush, shore rush,
3-square)
S. validus
TSoftst em bulrush,
Great bulrush)
up to 4 m high; coarse,
firm, dark green. Arising
from thick, creeping rhizome
without tubers. Flowers in
July/August; perennial.
Stem sharply triangular,
mostly solid; 2-5 mm thick,
up to 1.2 m tall. In
scattered groups arising
from long, thick rhizome
without tubers. Flowers in
July/August; perennial.
Stem soft, light green,
round, 1-4 m tall; 0.8 -
2.5 cm thick at base;
arising from stout creeping
rhizomes. Flowers July/
August; perennial.
except for southeast.
Common in hard water;
so-called "Tule marshes"
in calcareous regions.
Also in shallow water.
Widespread throughout
S. Canada and U.S.
Abundant in shallow
water or sandy shores
of lakes, ponds, and
streams; often in brack-
ish water eastward.
Widespread, Newfoundland
to S. Arkansas, south to
Georgia, Missouri and
California. In brackish
or fresh water up to a
meter or more deep, and
in wet meadows and marshes.

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TABLE 4.11. Continued (page 7 of 7).
Species
Growth Form
Distribut ion
Notes
4. Arum Family
(Araceae)
Acorus calamus
(Sweet flag)
Leaves and flowering stems
up to 1.5 m tall, in loose
colonies. Long, swordlike
leaves arising from stout,
creeping rhizome. Flowers
in June/July; perennial.
Widespread; Nova Scotia
and Quebec to Alberta
and Oregon; south to
Florida and Texas. In
marshes and edges of
lakes and streams. May
persist and even spread
in partially drained
marshy fields which
become relatively dry
in summer; flowering or
fruiting may not occur
in such a habitat, but
abundant quantities of
viable seeds are produc-
ed in shallow water or
spring-fed marshes.
Valued for its
aromatic fragrance;
used historically for
therapeutic and
related purposes.
The fragrance of tan-
gerines is due to a
volatile oil present
especially in the
rootstocks; the leaves
are also fragrant and
were often strewn on
floors. Used to
flavor beer, gin, and
other drinks; also to
scent perfumes and
cosmetics. See
Sculthorpe (1967).
5. Rush Family
(Juncaceae)
Juncus effusus
(Soft rush)
Stems up to 1.5 m growing
in tussocks, (of up to
several hundred) and arising
from stout rhizome. Flowers
July/August; perennial.
Throughout U.S. and
S. Canada, in meadows
and marshes.

-------
effect on redox (reduction/oxidation) potential and nutrient availability in
the rooting zone.
A number of studies have been conducted concerning the tolerance of aquatic
plants to submergence; a review of distribution and dominance of a number of
species as related to substrate, water level, and light preferences was
published by Spence (1967). Haslam (1970) discussed the biology, ecology,
and production of the common reed (Phragmites australis) as related to water
and water levels. Gorham and Pearsall (1956) presented information on water
level, pH, nitrogen, and organic matter content, and conductivity in reed
stands; they found that a declining water table was detrimental to
production, but depth of the water above the soil surface was not crucial.
Shoot production was greatest where mineral content of the water was high.
Cattails are generally dominant where the water level is greater than 15 cm;
optimum depth is 0.25 - 1.0 m. The reed is a poor competitor, but grows
well at water levels from 0.3 m below to 1.5 m above the soil surface and
often colonizes new areas. Scirpus olneyi (bulrush) requires water levels
from 5 to 10 cm above the soil surface in order to become established, and
is found at water levels of -0.75 m (below the soil surface) to +6 m; Sipple
(1979) discusses the biology, ecology, production, propagation, and
management of this species.
Production of the sedge Carex is decreased with increasing water level, and
this genus is generally found in shallower water than the bulrushes.
Generally, the grasses are found at the water's edge rather than in	standing
water; soils in cordgrass habitats are normally saturated, however,	and reed
canary grass has been reported to tolerate flooding as well (Broome	et al.,
1975; Byers and Zeiders, 1976; Bole and Bell, 1978).
C.4.b Effects of Temperature on Emergents—
Temperature tolerances for plant activity generally range between 0° to
45°C, differing with species and developmental stage. Temperate zone
species may require daily and/or seasonally low temperatures, while tropical
species may be grown at a uniformly warm temperature. The tropical plants
may be susceptible to chilling injury at above-freezing temperatures and be
killed at the first frost, but certain temperate species may freeze solid
without permanent injury. High temperature stress is reported to be more
dependent upon length of exposure than is cold-induced injury (Levitt,
1972).
The widespread distribution of most aquatic emergents attests to their
tolerance of a broad range in air temperature. The stabilizing effect of
associated water bodies on air temperature may also be a factor in this
wide-ranging distribution.
Most emergents require a cold period and the resulting dormancy. Aerial
shoots of Phragmites die in the fall, but rhizome growth is at a maximum in
early autumn and continues during the winter; dormancy may last from three
to six months depending on climate. Similarly, the successful cultivation

-------
of cattails, reeds, and sedges Involves harvesting the rhizomes In the fall
and storing them at 1° to 3°C for at least a month before planting. Alter-
natively, dormant rhizomes can be harvested during the winter (Dykyjova and
Veber, 1978).
Growth of reeds and sedges Is Increased at higher temperatures, and stands
are generally taller over a hot summer than over a cooler summer. The opti-
mal air temperature for root production for a temperate species of bulrush
Is 10°C, while a tropical species of bulrush requires 20°C. Similarly,
yield of a temperate species of Carex is greatest at 15°C, while the optimum
for purple nutsedge (Cyperus rotundus) found in warmer climates, is 32°C
(Wills, 1975; Gomm, 1978). Tuber development of purple nutsedge Is also
greatest at 32°C; ten-day lethal temperatures are 0° and 45°C (Palmer, 1961;
Horowitz, 1972). The tubers of the nutsedge will not sprout when the
temperature is below 19°C. Additional information on the temperature
requirements of purple nutsedge is reported by Shamsi et al. (1978). Reed
canary grass grows well at air temperatures of 15° to 30°C; requirements for
cordgrass are reviewed by Turner (1976) and Teal (1962).
Water temperatures of 30°C are optimal for the growth of cattails, but
survival is poor at 35°C (Jones et al., 1979). Vlehlova and Pribil (1978)
reported water temperatures of 11° to 16°C in Czechoslovakian reedswamps
through the growing season, and between 3° and 4°C in winter. No informa-
tion was found on the effects of water temperature on sedges or on grasses.
Soil temperature requirements of cattails have not been described, and no
work has been reported on aquatic grasses; however, reeds and sedges have
been studied. Haslam (1970a) presented much information regarding the
propagation of Phragmltes and found that: (a) horizontal rhizomes are
undamaged by frosts; (b) buds of vertical rhizomes (formed at the end of
horizontal rhizomes) are frost-sensitive and give rise to aerial shoots; and
(c) lethal temperatures vary with season.
C.4.c Light Intensity—
The effect of light intensity on cattails, rushes, and cordgrasses is not
known, but sedges, reeds, and canary grass have been studied. Optimum
illumination for purple nutsedge at 32°C is 100,000 J/m -h light energy
(Gomm, 1978). Shading by taller shoots inhibited reed growth (Haslam, 1972,
1973), but forced new shoot development in bulrush (Ondok, 1972). Purple
nutsedge tubers were shown to sprout more frequently in light than in dark
(Shamsi et_ al_., 1978).
Yield of reed canary grass grown at 15°C increased as the irradiance was
Increased from 15,000 to 150,000 J/m -h, but low light levels (15,000
J/m -h) and high temperatures were associated with nitrate and ammonium
accumulations to levels which could become toxic to livestock (Gomm, 1978;
1979).

-------
C.4.d Photoperiod—
The effects of photoperiod on reeds, rushes, bulrushes, and nutsedges are
not known. McNaughton (1966) showed that cattail rhizome production was
greatest with short days (12-14 hr versus 16 hr) and day/night temperatures
of 21°C/7°C. Tuber formation in Carex spp. is induced by short days (10 hr)
and inhibited by long days (18 hr~5 (Horowitz, 1972).
Vegetative growth of aquatic grasses has also been shown to respond to
changes in photoperiod. A combination of long (16 hr) days and day/night
temperatures of 30°C/25°C promoted aboveground production in reed canary
grass; below ground production was stimulated by short (9 hr) days and lower
temperatures (Sato and Audo, 1976). Cordgrass seedling bioraass was greater
with longer (12 hr) day length, while underground production and increased
chlorophyll content was induced by a nine-hour photoperiod (Seneca and
Broome, 1972).
In general, a short-day (9 to 10 hr) photoperiod is probably optimum for
underground production in the grasses and sedges, while 12 to 14 hours of
daylight are needed for below ground development in the cattail.
C.5 Plant Growth Characteristics
C.5.a Assimilation of Nutrients and Yield—
Annual mean production in a temperate Typha marsh is about 23 tonnes dry
weight/hectare-year (t/ha-yr) and 76 to 96 t/ha-yr in a reed swamp (Cyperus
papyrus) (Westlake, 1963; Thompson, 1972). Sculthorpe (1967) presented data
estimating ash content in floating and emergent communities at 11.5 percent
(i.e., 88.5% organic matter) on a dry weight basis. This estimate results
in organic matter (OM) production of about 20 t/ha-yr for Typha and from 67
to 85 t/ha-yr for Cyperus marshes. These values may be compared with
organic matter production of temperate and tropical agricultural annuals (20
and 27 t/ha-yr, respectively), and of temperate and tropical submerged
macrophytes (5 and 15 t/ha-yr, respectively) (Westlake, 1963). It is
possible that continuous availability of nutrients in a wastewater treatment
NFT could result In substantial yield increases over those reported above.
Pearsall (1920), however, gave a considerably wider range of organic matter
content in emergents (Table 4.12), with Carex spp. having the highest per-
cent CM and Typha latlfolla the lowest, 89.4 and 34%, respectively. These
high ash contents lead to dissolved salt management potentials with the NFT.
Boyd and Hess (1970) found that standing crop (dry weight biomass) of Typha
was strongly correlated with the dilute and soluble phosphorus content of
the hydrosol (the saturated or submerged soil) and the water standing above
the hydrosol. They also found significant correlations between environ-
mental levels of several nutrients (N, P, Ca, Mg, K, Na, S) and tissue con-
centrations of those nutrients. Tissue concentrations rose faster than
standing crop (considerably more than 50% net mineral accumulation occurred
before 50% of the maximum dry matter crop was produced) (Boyd, 1970).
51

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TABLE 4.12. ORGANIC MATTER CONTENT OF SOME EMERGENTS
(From Pearsall, 1920).
Emergent	Percent Organic Matter

Mean
Range
Typha latifolia
34.0
20-50
Scirpus and Phragmites
44.7
20-65
Equlsetam fluvlatile
60.9
40-80
Carex spp.
89.4
80-95
The chemical composition of emergent aquatic plants is directly related to
the composition of the substrate in which they grow, and thus is a valuable
Indicator of the capacity of these plants to remove various elements from
wastewater. Most data are reported for aboveground biomass only; ranges of
nitrogen and phosphorus content are presented in Figures 4.4 and 4.5, and
the percentage of those and other elements in emergent shoots are listed by
Stephenson et al. (1980). Heavy metal content of cattails, reeds and
rushes, sedges, and grasses is also given by Stephenson et^ al. (1980).
Mineral and OM content vary with season and specific organ. Younger tissues
are generally found to have higher CM content and mineral concentration than
older tissues, and for that reason multiple harvesting has been considered.
It is also known that in Phragmitest sodium, calcium, and magnesium tend to
be accumulated predominately by underground organs, but nitrogen, phos-
phorus, and potassium are distributed more evenly throughout the plant
(Ulehlova et al., 1973). N, P, and K are released to the surrounding medium
by senescing tissues, while Na, Ca, and Mg are translocated to shoot
tissue. Thus, for most efficient removal of nitrogen and phosphorus, it is
recommended that aboveground portions be harvested periodically (prior to
senescence). In order to maximize development of the root mass, which in
Phragmites generally lives for more than three years, it appears to be most
efficient to harvest aboveground portions periodically and to harvest the
entire plant after the end of the third growing season. This would, of
course, require a rotating system of cultivation with one-, two-, and
three-year-old plants. Actual long-term life cycle and propagation studies
of Phragmites in the NFT have not been done, so additional cultivation
systems might work successfully.
Cattails are characterized by a two-year growing cycle. Most of the shoots
are fertile in the second growing season, and rhizomes connecting these
shoots are dead at the end of that year. Cattails exhibit two periods of
maximal biomass Increase; one from July to early August, with peak incre-
ments in rhizome biomass and leaf area; and one from late September to
October, when there is an accumulation of reserves in the underground organs
(Fiala, 1973). The relative growth rate of roots exhibits two maxima
52

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(summer and autumn), but that of underground shoot bases and rhizomes peaks
only once in summer. The rate of Increase in bulk density of first-year
Typha rhizomes increases markedly from August through October; this increase
also occurs in both new and old rhizomes during that period of the second
growing season but is less marked. This phenomenon is associated with the
mobilization of reserves to those portions of the plant.
Estimates of yield have been compiled for a number of emergents, including
the cattails, reeds, rushes, sedges, and grasses. Figure 4.6 illustrates
the variations in peak aboveground biomass that can be expected under
natural conditions. These data show that in natural stands the cattails and
reeds can produce the greatest aboveground biomass. Similarly, peak below-
ground biomass in natural habitats is greatest in the reeds, rushes, and
certain grasses (Figure 4.7) and may be twice that of the aboveground
yield. Note that in an NFT the root mass would also be harvested. Reported
values for peak biomass (aboveground and belowground) are slightly lower for
both cattails and reeds in hydroponic culture; however, the reeds appear to
be a promising group in terns of yield for NFT. It appears that harvesting
the total plant (plant shoots and roots) in an NFT could result in total
yields exceedig 100 tonnes/ha-yr.
Variations in yield are attributable to a number of factors, including day
length, temperature, and length of the growing season. McNaughton (1966)
found that cooler temperatures and shorter days promoted rhizome development
in Typha and that annual production was greater at low altitudes than at
higher elevations.
Periodic harvesting reduces the annual yield of cattail, but less so in
artificial systems than in natural marshes (Spangler et al., 1976a; Spangler
et al., 1976b). Cordgrass grown in a marsh receiving treated municipal
wastewater showed 13 to 41 percent greater biomass than when grown in a
marsh not receiving wastewater; decomposition of litter was more rapid in
the enriched marsh than in the natural marsh (0.4 and 0.2 percent per day,
respectively) (Marshall, 1970).
C.5.b Release of Organic Carbon by Emergents—
Wetzel and Manny (1972) found 0.05 to 25% of the photosythetic carbon fixed
was excreted by a submerged aquatic, Najas flexllis, while a much lower per-
centage was released by the floating macrophyte, Lenma perpusilla. It is
likely that low rates of release of organic carbon occur in emergent species
because a large proportion of the photosynthate is contained in thick
celluloslc cell walls; this tissue is present in much lower quantities in
submerged species which can consequently "leak" more dissolved organic
compounds. Wetzel (1969) found that organic compounds released by
macrophytes are taken up quickly by periphyton (sessile algae and
bacteria). Of course, leakage of organic matter in a water treatment system
would be of prime concern.
55

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C.5.c Life Histories—
In studies with Phragmites communis, Has lam (1973) considered plants with
ca. 10 cm shoots to be "established." Each successive shoot produced from
the rhizome is generally taller and from a longer rhizome section than the
one previous; thus, a 10-shoot plant is usually over 30 cm high, enabling it
to survive about 20 cm of flooding. The rhizome can survive a certain
amount of drought, with enough reserves to tolerate some grazing or cut-
ting. Depending on environmental factors, seedlings may take from three
months to three years to reach this 10-shoot stage, before which mortality
is high; for this reason the plant is more easily established on rich sedi-
ments than on nutrient-poor media.
Propagation is readily achieved by placing rhizome sections in a moist soil
environment. Dykyjova et al. (1971) reported that seedlings require two or
three years growth before producing mature, flowering shoots. A three-year-
old clone of the plant occupies an area of about 4 m in diameter and is
capable of spreading 1 m/yr in ea^i direction. Density of natural stands is
usually about 100 to 200 shoots/m ; shoots may arise from rhizomes of all
ages.
The life history of the common cattail (Typha latifolia) is reviewed by Yeo
(1964). Generally, cattails are found in wetlands throughout North
America. They grow better in fresh water but can tolerate moderately salty
water and grow well when water depths range from ground level to 0.5 meter
above ground level.
C.5.d Effects of Harvesting on Yield and Chemical Composition—
Corns (1974) showed that for Carex atherodes, increased frequency of har-
vesting resulted in a lower annual yield (plots harvested six times per year
yielded only 40% of the biomass as those harvested twice annually); however,
total protein produced annually in plots harvested six times per year was
nearly 84% that of plots harvested two times per year. Van der Valk and
Davis (1978) found that removal of litter from a Carex stand increased
density four-fold.
Reed canary grass yield was not affected by frequency of harvest, but pro-
tein content was (Asay et al., 1968). Clipping height was found to be a
factor in regrowth of this species; yield was greatest when the grass was
clipped to 10 or 11 cm heights (Davis, 1960). Differences in forage quality
(lignin and nitrogen content) were found not to be affected by height at
harvest in that study. Lawrence and Ashford (1969), however, obtained
highest yields from reed canary grass and two other forage grasses when they
were cut at 3.8 cm height during the seed stage of development. Persistence
of the grasses was low at this cutting height.
C.5.e Regeneration and Reproduction—
Yeo (1964) showed that female spikes of Typha latifolia produced over
222,000 seeds, but that germination was relatively poor unless the cells
along the transverse edge of the seed were damaged by pressure (in which
58

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case 100 percent germination occurred). One Typha seedling matured to cover
an area about 3 m in diameter, with 98 aerial shoots and 104 lateral buds,
within one growing season.
Phragmites austrails spreads mainly by rhizome growth; it generally exhibits
poor seed germination and high seedling mortality in the field (Haslam,
1971). Haslam (1970b) showed that a young clone established by a seed or
rhizome can advance up to 2 m per year and produce up to about 200 shoots
per m in this advancing zone. Stands of Phragmites were reported to be
harvested regularly once a year in the Danube delta without harm to the
plants (Rudescu et al., 1965).
C.6 Metabolic Activity of Bacteria on Roots
Seasonal changes in microbial populations of a Phragmites stand were found
to be related to concentrations of dissolved organic nitrogen and ammonia
nitrogen (Ulehlova et al., 1973). A small microbial Increase in May was
followed by a second, more significant increase in late June; the concurrent
decreases in dissolved organic nitrogen and ammonia nitrogen suggested that
these compounds supplied a portion of the bacterial requirements for nitro-
gen. Dissolved organic nitrogen concentrations were fairly high throughout
the study period, however.
Inorganic nitrogen was present in extremely low concentrations in April and
May, reflecting high rates of uptake by algae and macrophytes. Nitrite
concentrations in the vegetated areas were considerably lower than in open
water areas throughout the growing season. Nitrate concentrations increased
considerably during late June and Increased slowly thereafter. Ammonia
nitrogen concentrations showed the greatest changes during the growing
season. Maximum concentrations in August reflected the high rates of
decomposition (ammonification) taking place at that time.
59

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CHAPTER V
SCOPE AND METHODOLOGY
The activities reported here describe a three-year +• feasibility project
of a unique hydroponic wastewater treatment system. The efforts in the
first six months, referred to as Phase 0, were designed to test the original
hypothesized system at a significant scale to identify the potential of the
system as well as the major limitations. A subsequent fifteen-month period,
referred to as Phase 1, included further experiments to determine the
feasibility of this plant based aquatic treatment system. This was a
cooperative effort of several groups and sponsors. The objectives,
participating groups and research activities are described below.
A.	PARTICIPANTS IN THE PROJECT
In Phase 0, three pilot scale experimental systems were constructed. These
systems were used to define major controlling variables that assist in
defining plant response to various levels of pollutants in the NFT system.
General Electric Company tested the NFT with a well defined inorganic plant
fertilizer and water solution in a total controlled environment greenhouse
with artificial lighting. Cornell University operated with a similar plant
nutrient solution which was modified to simulate a primary settled domestic
sewage. A primary settled domestic wastewater was used in the CRREL
greenhouse.
In Phase 1, studies at Cornell and in New Hampshire were continued in the
Phase 0 mode. Cornell assumed responsibility for plant culturing work which
was vacated by General Electric. The NFT project at Cornell was expanded to
include two, and at times, three, greenhouses at the Ithaca campus. The
original greenhouse continued operation with the synthetic wastewater
solution, while the second and third were used for multispecies plant
response studies and plant propagation. CRREL continued sewage testing in
its greenhouse. In addition, a large greenhouse was constructed at the
Hanover, New Hampshire, treatment plant to house larger pilot scale and
plant testing. Some sample analyses of the pilot scale unit were conducted
by CRREL personnel while plant culturing in sewage work was done by
Cornell.
B.	METHODS TO MEET THE PROJECT'S OBJECTIVES
The overall goal of the project was to define a cost-competitive and effi-
cient treatment system using the nutrient film technique (NFT). Toward this
end were the following objectives:
60

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1.	identify and characterize plants capable of performing sewage
treatment;
2.	establish systems In which these plants can remove pollutants
efficiently;
3.	characterize the performance of these systems;
4.	develop design criteria for larger systems.
To meet these objectives, two basic methodologies were used. First, plant
response to the NFT system itself was tested using a well-defined inorganic
plant nutrient and tap water substrate. Second, pilot scale NFT units were
constructed to test sewage treatment using real or synthetic sewage.
Throughout the course of experimentation, many different plants and system
configurations were evaluated to get the best estimates of plant and system
performance. Plants ranged from wetland and aquatic plants to forages and
tropical plants, and eventually to ornamental and horticultural plants.
Pilot scale systems ranged from 12.2 to 36.6 m (40 to 120 feet) in length
and treated between 0.47 and 11.36 m^ (125 and 3000 gallons) per day. A
chronology of the major research activities is summarized in Figure 5.1.
C. PHASE 0 ACTIVITIES
C.l Cornell University
The tasks of the Cornell University team for Phase 0 were to estimate
organic and nutrient removals by the NFT system and examine the kinetic
effects of increasing loading rates on the NFT system. During Phase 0, the
dally application rate was 10 cm (4 inches) per day, and this daily rate was
not increased. However, data were collected from intermittent loading
cycles. These included loadings of four to eight times the Instantaneous
flow rate of continuous application, and Included long periods of draining
to promote root zone aeration.
Studies at Cornell were conducted with a synthetic wastewater feed equiva-
lent to a medium strength domestic primary sewage. This substrate also con-
tained a known level of cadmium for study of the fate of an Important heavy
metal in the NFT system. This portion of the work was conducted in parallel
with studies on domestic sewage conducted in New Hampshire.
This work at Cornell resulted in development and testing of original designs
for NFT facilities, including trays for plant growth, dosing systems, sampl-
ing stations and management procedures for maintenance of these facilities.
The Cornell pilot scale unit developed in Phase 0 is shown in Figure 5.2,
along with those developed and tested at 6E and in New Hampshire. Exper-
ience was gained in propagation of reed canary grass which was used in all
Phase 0 w>rk at Cornell and CRREL. Data were collected for over three
months on organic removals, nutrient transformations, heavy metal uptake,
plant yield, oxygen relationships and environmental conditions.
61

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Project
Phase
CornelI
Activit ies
Activit ies
at New
Hampshire
GG
Activities
A Funding Start
Phase 0
A Funding Extension
Phase 0 Report
Phase 1
Phase 1 Report
Preliminary
Discussion
Greenhouse
Sys. Design
Phase 0 Pilot
Scale Testing
Phase 1 Pilot
Scale Testing
Green-
House Con- Large Unit Pilot
struction Scale Testing
Plant Culturing & Assessment
Phase 0 Pilot
Scale Testing
Phase 1
Testing
Greenhouse Large Unit Pilot
Construction Scale Testing
Plant Culturing
I I I
June
1979
Oct
Feb.
1980
June
Oct
Feb.
1981
June
Oct
Figure 5.1. Chronology of major events and testing periods of the NFT project.

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A.	GENERAL ELEC.
B.	CORNELL, NFT PROJECT
C CRREL , NFT PROJECT
A.
NUTRIENT STORAGE TANK
6m
!¦ I.) W','	'rlviMM ididh
NUTRIENT REC. LOOP
5
SURFACE AREA = 6mc
VERY SLIGHT SLOPE «1%)
B.
^ 9	°	12.2m	/
SEWAGE TAP	y
0.76 m
°; CONC. WATER MIXING CHAMBER

PRIMARY
SEWAGE
EFF WASTED
EFF WASTED
SURFACE AREA = 93m'
SLOPE = 2 %
SURFACE AREA= 7.4m2
SLOPE = 5%
Different NFT systems at Cornell University, Genera
at CKKEI. in Hanover, New Hampshire.
1 Electric Company Syracuse facility, and

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C«2 New Hampshire
In Phase 0 the main task of the New Hampshire unit operated in the CRREL
greenhouse was to determine the response of reed canary grass to domestic
sewage. Tests were similar to those performed at Cornell. A range of flow
rates was used, with all flowB being intermittently applied. Again, reed
canary grass was the test species. The analyses performed on the wastewater
Included gross organic and nutrient determinations as well as yield,
hydraulic retention time, and environmental conditions.
C.3 General Electric
The principal task of the General Electric Controlled Environment Agricul-
ture Operation (CEAO) was that of defining cultural methods and nutrient
responses. The unique characteristic addressed was the presence of
relatively low levels of nutrients. Five plant species were tested for
their adaptability and assimilation. These included reed canary grass,
coastal bermuda grass, napier grass, watercress and cattails. Sufficient
quantities of these were propagated to estimate nutrient uptake,
transpiration rate and blomass production. Unlike the Cornell and CRREL
"one pass" systems, which were designed to monitor mass and concentration
changes, the solution depth and flow rate were not critical in the GE
recirculated systems. Due to the highly synthetic nature of this portion of
the study, no data will be reported in this final report on this phase of
the study.
D. PHASE 1 ACTIVITIES
D. 1 Cornell University
Testing continued on the canary grass units similar to testing done in Phase
0 at Cornell in Phase 1, but the range of loading rates was increased up to
a peak of 40 cm (16 Inches) per day. Canary grass units were joined end to
end to test the effect of longer units, and grass was transplanted Into the
units and harvested. Since this phase of the project Included spring and
summer months, testing the units under more favorable light and temperature
conditions than in Phase 0 was possible.
In addition the Cornell team assumed the plant culturlng responsibilities
vacated by General Electric after Phase 0. Many potentially suitable plant
species were identified and either grown from seeds or stock, or collected
in various forms from the field. These plants were tested for their adapta-
bility and productivity in small NFT systems. Many of them were tested in
carbon free solution to determine their nutrient uptake rates. Some plants
were also tested in recirculating sewage systems to assess their uptake
rates under organic loadings. A wide variety of plants was grown in the
one-pass sewage system in the Hanover greenhouse. These growth trials
allowed conclusions to be made concerning plant suitability and performance,
and identified management techniques necessary for operating a large
system.
64

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A large (120 foot) multispecies unit was tested extensively at four distinct
loading conditions, ranging from 6.9 cm to 40 cm (2.7 to 16 inches) per day,
using the synthetic sewage. The different regions of this system repre-
sented the various parts of the hypothesized NFT treatment system—roughing
filter, nutrient stripping and polishing steps. These experiments were
paralleled using domestic sewage at the Hanover treatment plant.
Smaller scale experiments were undertaken in two areas: first, performing
a closer examination of the root zone to assess the effects of root surface,
water depth and associated growth around the roots, and second, to design
a system to perform significant organic and solids removal in a small area.
This second activity used trays that could contain up to 12 inches of waste-
water which were periodically flooded and emptied. This activity was
carried out at the Village of Cayuga Heights (New York) sewage treatment
plant in the town of Ithaca, New York.
D.2 CRREL
At the start of Phase 1, CRREL continued testing the pilot scale reed canary
grass unit with sewage. Several additional loading conditions were tested.
Along with the Cornell team, CRREL personnel set up and operated the green-
house at the Hanover treatment plant, which tested loading rates from 5 to
20 cm (2 to 8 inches) per day. Extensive analyses, similar to those run at
Cornell, were completed in the CRREL laboratories. In addition to other
analyses, these units were tested for trace organic removal, with the
analysis being conducted by CRREL.
65

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CHAPTER VI
MATERIALS AND METHODS
The goal of this study was to define the conditions that would enable the
application of a solar-powered plant system to be used as a water purifica-
tion system. This broad-scoped feasibility study thus required a wide range
of variables to be evaluated—a variety of plants needed to be grown with
defined substrate and with "real world" sewage; the scale of the experiments
had to be large enough to enable extrapolation to full scale; and experi-
ments had to be long enough to reflect seasonal influences as well as
senescence problems when used for several years. ^ant systems have been
tested for up to three years, flow rates up to 11 m /d (3000 gallons per
day) have been applied with a synthetic wastewater and sewage enabling
comparison of effects of unknown constituents in sewage to a defined situa-
tion. NFT units were established in four separate locations in New York and
New Hampshire. The definition of the approach is given in this section.
The total time frame for this study was three years, with most of the exten-
sive testing completed in a two-year time space in the middle of the
program. The first eight months of this effort were referred to as Phase
"0" and were intended to be a confirmation of whether the NFT could be
adapted to wastewater treatment. These preliminary tests were followed by
more comprehensive testing in a longer period referred to as Phase I.
A. PROCESS DEFINITION WITH SYNTHETIC SEWAGE - STUDIES AT ITHACA, NEW YORK
A.l. Synthetic Sewage Composition
To gain better control of influent variables, the Cornell systems utilized
a synthetic medium strength domestic wastewater. A previously developed
formula (Switzenbaum and Jewell, 1978) was modified to produce the following
theoretical concentrations:
Sucrose was added as the carbon source. Commercial silage urea was used as
the nitrogen source. The carbon added as urea was negligible compared to
that added as sucrose. The use of urea-nitrogen enabled estimates to be
made of the fate of all forms of nitrogen commonly found in fresh domestic
sewage. Phosphate was added as mono- and dibasic potassium phosphates. The
presence of both compounds contributed to the buffering capacity of the
COD
N
P
400 mg/£
40 mg/ I
15 mg/ I
66

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system, and the use of potassium salts served as a potassium source to the
grass. In addition to these constituents, yeast extract was added as a
trace element source. To further increase trace nutrients, tap water was
used for all solutions. Cadmium sulfate was added to produce a
concentration of 0.2 rag/I cadmium (Cd) in the simulated sewage. Some
difficulty was experienced in controlling the cadmium concentration.
Dry chemicals were weighed and dissolved into concentrated solutions which
were diluted with tap water to produce the wastewater. Chemicals were
dissolved in 201 of tap water, diluted to 1001 in the feed storage tank, and
further diluted 15 times in the distribution box. The amounts of dry
chemicals dissolved in 201 of tap water, diluted to 1001 in the feed storage
tank and further diluted 15 times in the distribution box. The amounts of
dry chemicals dissolved in the 20 & concentrate are shown in Table 6.1.
TABLE 6.1. DRY CHEMICAL CONSTITUENTS IN CONCENTRATED FEED STOCK
AND SYNTHETIC SEWAGE
Const ituent
Stock
Concentrat ion
g/201
Sewage
Concentration
mg/ I
Major
Constituents
mg/ I
Sucrose
536
357
COD-400
Yeast Extract
3
2
	
Urea
130.5
87
N-40
KH2P0i+
75
50
P-ll
K 2HPO1+
30
20
P-4
CdSOit
0.686
0.46
Cd-0.2
Total feed concentrate storage volume was 200 1. This provided more than
three days' storage time at the given withdrawal rates. This time period
was long enough to minimize labor in feeding the system, while being short
enough to minimize problems of changes in feed composition due to microbial
activity and/or chemical hydrolysis.
The initial formulations of the feed mixture also included sodium bicarbon-
ate as a buffer. However, the addition of this constituent resulted in the
formation of an inorganic precipitate in the distribution box and among the
grass roots. Its formation was particularly noticeable under warm condi-
tions. Due to this precipitate and the lack of an apparent need to further
buffer the system, this constituent was deleted from the formula.
At the start of the testing program, the wastewater was added continuously
at a rate of 655 mi/minute, which corresponded to a loading of 10 cm/day.
This flow was made up of 611 mi/minute of tap water and 44 mi/minute of feed
stock. In subsequent intermittent applications, the total application time
was six or three hours per day. Additional information on loading schedules
67

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is given later. Since Che total application remained 10 cm/day, the flows
during the application periods were 2650 and 5300 mi/minute, respectively.
The mixing ratio of tap water to feed concentrate of 14:1 remained the
same. All subsequent loading conditions used some variation of a feed
concentrate—tap water mixture.
The concentration of the stock concentrate solution could be increased up
to eight times without precipitating some constituents. This allowed in-
creased loading rates up to 81 cm/day without an increase in the feed stor-
age volume or feed makeup frequency. Rather, the mixing ratio in the dis-
tribution box was increased. This also provided flexibility in application
rates and feed storage periods. At high loading rates, it was necessary to
increase the feed pump rate and prepare feed concentrate more frequently.
For the purposes of maintaining seeded and sodded control systems, a
solution of Peter's brand soluble fertilizers was used in recirculating
systems. A mixture of Peter's Hydrosol (mixed nutrient) and Ca(N0 3)2
fertilizers maintained adequate levels of N, P and K. Sequestrene chelated
Fe III was added periodically to reduce iron deficiency in the grass.
During periods of high light intensity or low foliar cover, algae buildup
raised pH values. This was controlled and/or corrected by additions of
copper sulfate or nitric acid. These additions occurred only in the
background phase of work, and all of the data were taken from units which
were not being treated for algal growth. As will be discussed, algae growth
is a major problem in propagation and start-up.
In operating the 1.5 meter (5 foot) bench scale unit in Ken Post labs, the
feed solution was modified. The unit used 7.5 £ reservoirs, and the solution
was recirculated, so only a small amount of synthetic wastewater was needed
at the start of each experimental run. The following amounts of chemicals
were dissolved in the 7.5 £ reservoirs to produce identical concentrations to
the synthetic wastwater:
NH i+Cl 1.148 g
K 2HPO 0.33 g
KH2P0i+ 0.21 g
Yeast 0.75 g
Sucrose 1.379 g
The carbon source sucrose was added in only those experimental runs where
removals of BOD, COD, and solids were of interest. Since very small amounts
of Cadmium (Cd) would have been difficult to add, 10 m £ of a stock CdSOi*
solution were added to produce the correct concentration.
An additional experiment with the 1.5 meter (5 foot) bench scale unit
evaluated BOD removal at various loading rates using reed canary grass and
required a one-pass through flow of wastewater. A 627 A (165 gal.) reservoir
of synthetic wastewater was a common source of feed for each of the six
troughs on the unit. The following amounts of dry chemicals were dissolved
in the reservoir:

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K 2HPO
KH 2PO
Yeast
Sucrose
Urea
3 CdSO i* - 8H 20
54.9	g
12.6	g
31.5	g
1.2	g
130.7	g
0.285	g
A.2. Pilot Scale Units (Bracehouse)
A.2.a. Brace greenhouse and test units—
The NFT work at Cornell is being conducted in an asymmetrical greenhouse
with a large, inflated polyethylene south wall and a smaller, opaque, insu-
lated north wall. This greenhouse is designed to maximize solar energy
input and conservation in colder climates and is patterned after the orig-
inal design of this type developed at the Brace Institute (Laward et al.,
1975). The Cornell Brace-style greenhouse is illustrated in Figure 6.1.
(Albright et al., 1978).
The greenhouse has floor dimensions of 7.3 x 14.6 meters (24 x 48 feet), and
contains four benches, each 0.76 x 12.2 meters (2.5 x 40 feet). The north
wall is shingled, contains 14 centimeters (5 1/2 inches) of fiberglass
batting, and is lined on the inside with white polyethylene to enhance light
reflection to the benches. The roof peak is 4.6 meters (15 feet) from the
floor, and the south wall angle is 38°. Two 27 megajoule (7.5 kw) heaters
at floor level provide enough supplemental heating to maintain a night
temperature of about 12°C (54°F) under winter conditions. An automatic
ventilation system maintained the temperature below 35°C.
A third greenhouse was used for a short period for plant growth and stor-
age. In a joint project, the departments of Agricultural Engineering and
Floriculture and Ornamental Horticulture built a 12.2 x 30.5 meter (40 x 100
foot) air-supported, cable-restrained greenhouse. The covering material was
a double layer of Loretex woven polyethylene with ultraviolet stabilizers.
Although the air-supported nature of the greenhouse required four air
changes per hour, the temperature range was maintained between 15.6 and
23.9°C (60 and 75°F).
Work begun in the Cornell greenhouse on June 4, 1979, was geared toward
gaining experience with establishment of reed canary grass in the hydroponic
(NFT) units. Both transplanted sod and grass germinated from seed were
investigated to determine the most efficient means of establishment so that
testing could begin within the time constraints of the project. Results of
this work, which resulted in the use of transplanted sod rather than seeded
grass, are discussed in more detail in a later section.
Also developed during this phase of work were NFT trays to contain grass
and distribute the nutrient film flow, distribution boxes to mix and distri-
bute influent to the NFT units, storage and pumping systems to feed the
69

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Souih WalI -Air Inflated double
layer polyethylene
-J
O
White polyethylene
liner to reflect light.
North Wall
Insulation
End Walls-corrugated fiberglass
Under bench rock bed
for solar heat storage
Kitfure 6.1. The Cornel l braee-atyle Liohir ;<;reeiiliou:.-.e

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units, and procedures for routine maintenance of the facility. During the
latter part of the summer, after grass establishment, regular harvests were
made of the foliar production by hand-clipping the foliage to a level of
four inches above the roots. Records of the fresh and dry matter yields
were kept and are reported in the Results section of this report.
Beginning work about four months in advance of project funding enabled the
Cornell group to establish a facility with a mature crop ready for testing
at the beginning of the project. This background work assumes more impor-
tance when it is realized that none of the equipment or procedures were
packaged or ready-made but had to be designed to fit the existing facili-
ties. Through trial and error, the experimental setup evolved from the
initial designs so that it has performed with very little maintenance and
has been readily adapted to different operating conditions during the test
phase of the project.
The following sections describe various parts of the NFT system in operation
at Cornell. This experimental setup is shown in Figure 6.2.
The general concept of the NFT units is a system of 10 trays in series with
the provision for recycling flow to a holding tank or direct disposal of
effluent. The trays have a 2 percent slope. The Cornell units conform to
this schematic plan except that they are 0.76 m x 1.22 m (2.5 x 4.0 ft) [9.3
m (100 ft ) surface area] due to space limitations in the greenhouse.
A design incorporating 10 trays, each 1.2 m (4 ft) long x 0.76 m (2.5 ft)
wide and made of wood and plywood, was produced. Between each successive
tray section was a two inch drop to the next tray, providing aeration of the
wastewater in these cascades. These were built beginning in June 1979. The
standard tray is shown in Figure 6.3. Trays were lined with polyethylene
and overlapped to form the unit. Supports consist of wooden blocks fitted
with threaded rods. These are shown in Figure 6.4, a section view of the
tray, which also illustrates the sampling ports.
Sample ports were placed at intermediate points in the unit by drilling
through the 5 x 10 cm tray support, cutting a hole in the plastic liner and
inserting plastic tubing, flush with the tray floor. Ports were installed
at points 3.7 m and 8.5 m from the distribution box, and these are referred
to as points A and B, respectively. Two sampling ports were installed at
each point. The two-inch drops between trays were removed in 1980 since
Phase 0 had shown that little aeration took place in these cascades.
In December 1980 another 12.2 meter (40 foot) unit was disassembled in
preparation of building a level unit able to withstand flooding by up to
30 centimeters (12 inches) of synthetic wastewater. Greenhouse benches
supporting the original sloped unit were joined end to end; 46 centimeter
(18 inch) wide strips of 2 centimeter plywood was added to form sides; and
one end wall and a plastic liner were installed to contain the wastewater
and plant roots. The completed system is shown in Figure 6.5.
Figure 6.6 is an overview of the interior of the Brace greenouse which gives
an overview of the units. The test unit of transplanted reed canary grass

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SCHEMATIC OF CORNELL NFT TEST UNIT
/'TAP WATER (CONSTANT
^	FEED)
FLOAT VALVE
CONCENTRATE BATCH TANK TAP WATER TANK
OVERFLOW
RETURN
SUBMERSIBLE PUMP
MASTERFLEX
PUMP
MIXING CHAMBER
INFLUENT SAMPLE POINT
0.76 m
TRAY I
3.7 m
INTERMEDIATE SAMPLE POINT A'
4.9 m
8.3m 12.2 m
INTERMEDIATE SAMPLE POINT "B
3.7 m
EFFL'JEi
SAMPUNG POINT
WASTE
EFFLUENT TANK
Figure 6.2. Schematic diagram of basic Cornell N7T experimental
unit.
72

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0.7<
2.0cm	: n
PLYWOOD
-i
LO

5cm xlOcm SUPPORTS
SLOPE
7.6 cm
7.8 cm
0.64cm
PLYWOOD
Standard NKT tray.

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PLASTIC
LINER
1.3cm 1 D
PLASTIC
TUGING
w
GRASS
\ ROOT ZONE
RUBBER STOPPERS
5cm x 10cm SUPPORT
ADJUSTABLE THREAOEO
ROD SUPPORT
CLAMPS
BENCH
Kiyure b.'i. Section view ot' NP'T tray, ;;ampJin
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Figure 0.5.
Level unit
flooding.
that can withstand
rigure 6.6. View of interior of Cornell solar greenhouse
showing N?T trays empty (right) and con-
taining reed canary grass (left).
75

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sod is on Che left, while the seeded reed canary grass unit is on the far
right. The empty middle unit illustrates the placement of plastic liner in
the trays.
Figure 6.6 also shows distribution boxes that were designed to achieve
influent mixing. The design of the influent distribution boxes is shown in
Figure 6.7.
Tap water supplied by a constant rate submersible pump entered the rear
chamber of the distribution box. A portion of this flow passed through a
30° V-notch weir into the front chamber, where the concentrate was intro-
duced and mixed with the tap water. This flow was regulated by a movable
flat overflow weir which set the water level in the rear chamber. Overflow
recirculated to the influent tap water barrel. The overflow weir level was
set manually, and a V-notch discharge weir provided a visual check on flow
rate. Figure 6.8 shows the system in operation, discharging to a gravel
filter which further distributed flow prior to entering the NFT reactor.
The gravel filter was used in this manner until development of a thick root
mass of grass rendered it superfluous, at which time it was removed.
In testing conditions where large instantaneous flows were required, distri-
bution boxes were not used because it was difficult to make fine adjustments
in large flows. Rather, tap water was sequentially passed through a pres-
sure regulator, solenoid valve and gate valve. The regulator insured the
same pressure was delivered to subsequent valves. The solenoid valve, which
was activated by cycle timers, controlled the tap water flow during inter-
mittent loading conditions. The gate valve controlled the actual flow rate
into the unit. In this setup, feed concentrate was introduced and mixed in
the tap water delivery pipe above the gate valve. The feed was delivered
from a concentrate solution by a Masterflex positive displacement pump, just
as in the distribution box system.
When more than a single 12.2 meter (40 foot) unit was being tested in a
sequential system, float-activated submersible pumps placed in catch barrels
were used to transfer wastewater from the foot of one unit to the head of
the next. During the two highest loading conditions it was necessary to add
a pump to the waste line.
Air temperature at bench level was monitored with a recording thermometer
(Taylor Instrument Company) which provided a permanent temperature record on
seven-day strip charts. The temperature records also provided a very reli-
able record of sunlight due to an almost instantaneous response in the
greenhouse temperature to sunlight above the baseline temperature of 12aC
(54®F). Virtually the entire testing period during Phase 0, and a signifi-
cant portion of Phase 1, coincided with outside air temperature in Ithaca
which did not reach 12°C (54°F), so any inside readings above this value
were due to sunlight. These data will be examined to determine if any
correlations exist between temperature and system performance.

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SIMULATED SEWAGE
CONCENTRATE
X
OVERFLOW RETURN TO TAP SUPPLY
MOVEABLE WEIR
INFLUENT TO NFT I
UNIT	Y
20cm
7 5cm
J
75cm
TAP WATER
BAFFLES
Figure 6.Y- Lk: hematic diagram of the infLuent distribution
box.

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Figure 6.8. View of synthetic wastewater mixing and
distribution system.
73

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A.2.b. Loading Rate Schedule and Plant Species Tested—
A variety of loading rates, unit lengths and plant species were tested in
the Cornell Brace house. In Phase 0 all testing used 12.2 meter (40 foot)
length units containing reed canary grass. These units were loaded at 5 to
20 centimeters (2 to 8 inches) per day (473 to 1890 liters per day), and
were operated at a variety of intermittent and continuous conditions. These
conditions are summarized in Table 6.2. In Phase 1 these series of experi-
ments continued and more data were collected on individual units. In the
summer of 1980 the third and fourth units were interconnected so that the
effluent from the third became the influent to the fourth. In this longer
system it was hypothesized that most COD removal would occur in the first
12.2 meters (40 feet), which would allow the second to perform as a nutrient
removal step. These systems used canary grass only. The schedules of test-
ing with single and double unit systems are shown in Table 6.3. Since the
Brace house normally contained four working units, there was space available
to perform culturing experiments, retention time tests and biomass compari-
sons in addition to the major analytical work. While the primary testing
units had canary grass derived from field collection of sod, the secondary
units were grown from seed. These units provided "clean root" comparisons
for retention time studies and growth comparisons for the test units. Grass
grown from seed in these units was eventually used in the 36.6 meter (120
foot) pilot units at Cornell and Hanover.
TABLE 6.2. PHASE 0 TESTING SCHEDULE FOR THE BRACE HOUSE 12.2 METER
(40 FOOT) CANARY GRASS UNIT. LOADING RATE OVER THE
TESTING PERIOD WAS 10 CENTIMETERS (4 INCHES) PER DAY,
OR 1.24 m /m-d (946 LITERS PER DAY)
Condition	Wastewater	Instantaneous Flow During
Starting Date	Application Mode	Application (Vmin)
12- 6-79	Continuous application	0.66
1- 3-80	30 minute loading,	2.62
90 minute pause,
Alternating cycle.
1-28-80	15 minute loading,	5.24
105 minute pause,
Alternating cycle
2-21-80	30 minute loading,	2.62
90 minute pause,
Alternating cycle.
79

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TABLE 6.3. PHASE 1 SCHEDULE FOR THE BRACE HOUSE 12.2 METER (40 FOOT)
AND 24.4 METER (80 FOOT) CANARY GRASS UNITS
Date
Unit
Number
Dally Loading
cm/day m /m-d
Liters
per Day
Application Mode or
System Setup
3-18-80
4
5
0.62
473
Continuous application
5-12-80
4
10
1.24
946
Continuous application
6-17-80
3
5
0.62
473
Continuous application
7- 4-80
3
20
2.49
1890
Continuous application
9- 9-80
3+4
10
2.49
1890
Effluent of 3 used as
influent to 4
10-16-80
3+4
20
4.98
3785
Effluent of 3 used as
Influent to 4
In the fall of 1980 the greenhouse units were rebuilt to contain a system of
three 12.2 meter (40 foot) lengths connected In series, with each unit con-
taining a separate plant species. The rationale for this arrangement was to
use the capabilities of different plants to the best advantage. The three
sequential units specialized in acting as a roughing filter for gross organ-
ics, a nutrient removal step and a polishing step, respectively; and each
contained a species suitable to the task. The first unit was built level
so that the water level could be raised, held and lowered on an intermittent
flooding regime. In fact the unit never operated in that mode, but the
level unit did provide for a larger volume of water in that unit and thus
a longer retention time.
The original selection of plants for this large system Included phragmltes,
cucumbers and canary grass for the roughing, nutrient uptake and polishing
steps, respectively. The three part system at Hanover, which was identical
to that at Cornell, is shown in Figure 6.9. After testing the first condi-
tion of 6.9 centimeters (2.7 inches) per day (1893 liters per day), it
became obvious that phragmltes and cucumbers were not suitable for location
In the roughing section. Phragmltes showed poor growth and a large buildup
of black material around the roots while cucumbers did not develop a large
root mat and plants did not appear vigorous. For the duration of the pilot
scale studies the first unit was loaded with 6.1 meters of cattails followed
by 6.1 meters of bulrush, and phragmltes were placed in the second 12.2
meters. The third 12.2 meter unit contained canary grass. A schematic
diagram of this arrangement is shown In Figure 6.10. The loading condition
regime proceeded to test 10.2, 20.3 and 40.6 centimeters (4, 8 and 16
inches) per day (2839, 5678 and 11356 liters per day) before returning to
repeat the 6.9 centimeters per day loading. The testing schedule for this
system is shown in Table 6.4.
80

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Flow
Me L c r
lope
c»
h-1
Kee(I Canary Gra,
	UnlhJ'S
Constant [''low
l'ump and
Weir Box
lope = 2%
l''i/-',ui'e 6.9. Schematic diagram ol' three-pat't 36.6 meter system at Hanover containing several species

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Teed
(.'oneonr rate
Pump
Mow Control
Valve „
Timer ——
Con Irol
	 Fe
Concen t1 ^
Re.servo i
F1 oaL
Va 1 vi!
ed
Cat ta i 1
Rti-1 rush
Wa r e r
Reservo i r
Barrel
Tap Water l,ine
Catch Barrel
I:
Float
Ac l. ivatod
Pump
Slope
Catch Barrel
V
Float Activated Pump
Slop
Catc 11 Bar re 1"
Wast e.
,ine
I'"l( ',nre 6. JO. Hehomat i <: ci i a jr.rani of Braeehouso 36.6 moU-:r multi species unit.

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TABLE 6.4. TESTING SCHEDULE FOR THE CORNELL 36.6 METER (120 FOOT)
MULTISPECIES SYSTEM
	Daily Loading	
Cm/Day Liters/Day
m3/m-d
Loading
Schedule
Starting
Date
Species

6.9 2.49
1893
Continuous
application
2-13-81
Phragmites, Cucumbers
Canary grass
10.2 3.74
2839
Continuous
application
4-16-81
Cattails/Bulrush,
Phragmites, Canary
Grass
20.3 7.47
5678
30 min. loading
30 min. pause
Alternating cycle
5-11-81
Cattails/Buirush,
Phragmites, Canary
Grass
40.6 14.94
11356
30 min. loading
30 min. pause
Alternating cycle
6-11-81
Cattails/Buirush,
Phragmites, Canary
Grass
6.9 2.49
1893
Continuous
application
7- 2-81
Cattails/Buirush
Phragmites, Canary
grass
10.2 3.74
2839
30 min loading
30 min. pause
Alternating cycles

Cattails/Bulrush,
Phragmites, Canary
grass
The remaining 12.2 meter unit in the Brace house was used to monitor evapo-
transpiration water use and as a biomass control. Cumulative water use by
this unit was measured twice a day by refilling the recirculating solution
to a level controlled by a float valve. Both this unit and the third unit
of the 36.6 meter system contained canary grass and underwent approximately
the same temperature and nutrient conditions, and both units were harvested
on the same days.
A.2.C. Root Analysis—
In an attempt to develop the relationship between the root surface area in
contact with the wastewater, biomass accumulation and subsequent organic
removal, root sampling devices were placed in the 36.6 meter (120 foot)
pilot scale units at Cornell and Hanover.
For cattails, bulrush and phragmites, the root sampling device was a
one-foot square basket made of 1.3 cm wire screen with 7.6 centimeter (3
inch) sides (see Figure 6.11). The basket was placed on a flat piece of
sheet metal located in the bottom of the NFT unit. Plants were then placed
83

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Figure 6.11. Root sampling devices: (a) cattail, "bulrush
and. phragmites, (b) reed canary grass.
8U

-------
in the basket at the same density as the surrounding plants. The sheet
metal plate had 8 screws mounted on it, so that a square sheet metal sleeve
could be slipped around the basket and clamped down to the bottom plate.
The entire sampling device was then extracted from the unit, including the
plants, attached biomass, and solids entrapped by the root mass.
For reed canary grass, a similar device was developed, except that it
incorporated a smaller surface area. A cylindrical 7.6 centimeter (3 inch)
diameter basket was formed from 0.64 cm wire screen with 7.6 centimeter
(3 inch) sides. The basket was placed on top of a 10.2 centimeter (4 inch)
square piece of flat sheet metal, with a threaded center post extending from
the sheet metal through the basket. This device was placed in the reed
canary grass unit, and filled with the grass at a density similar to that of
the surrounding grass. When sampled, a cylindrical sleeve was placed over
the basket and tightened down with a flat bar and nut on the threaded rod so
that the sample was extracted.
In the cattail/bulrush and phragmites units, three root sampling devices
were placed in each unit, one at each position of 3.0, 7.6, and 10.7 meters
(10, 20 and 35 feet) from the influent end. For each loading condition, the
basket at the 7.6 meter position was sampled half-way through the condition;
and the baskets at the 3.0 and 10.7 meter positions were sampled at the end
of each condition. After sampling, the baskets were repositioned at their
original locations with the vegetative contents intact.
In the reed canary grass, nine sampling baskets were placed, three at each
location of 3.0, 7.6 and 10.7 meters (10, 25 and 35 feet) from the influent
end. Basket sampling was similar to that for the cattail/bulrush and phrag-
mites, except that in addition to sampling a previously unsampled basket,
any baskets which had been previously sampled were extracted again. The
purpose of this was to see if having previously extracted a basket would
have any effect on the biomass accumulation.
After the baskets were removed from the units and the sleeve and bottom
plate removed, the basket was suspended in a pan with a known volume of
water and vigorously agitated to remove the attached biomass. This proce-
dure was repeated with new water until the water stayed clean, and the roots
appeared to be clean by visual inspection. Information collected from the
sampled baskets included: volume of water entrapped; total and volatile
solids contents of entrapped water and that collected from the root clean-
ing; weight of water and solids entrapped; estimated average root dimensions
(length and diameter); root mat thickness; and a qualitative description of
the roots (color, growth irregularities, etc.). Other data recorded were
the wastewater depth in the unit during application and rest periods; a
description of any algae or other growth in the wastewater; and a descrip-
tion of the top vegetative growth.
For reed canary grass, a volume displacement test was done to try to cor-
relate root volume or surface area with biomass entrapment and organic
removal. A similar approach was attempted for the cattail/bulrush and
phragmites, but the volume of water displaced was too small to measure due
to the large surface area of the one foot square sampling basket.
85

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A.3. Bench Scale Units
A.3.a. Ken Post Greenhouse and Test Units—
The NFT project used glass-covered greenhouse space in the Kenneth Post
greenhouse complex, which is under the direction of the Department of
Floriculture and Ornamental Horticulture. The project had use of house 6-C,
which is a glass-covered, corner greenhouse having a floor space of 7.6 x
7.6 meters (25 x 25 feet). The house was heated by steam radiators and
ventilated by two automatic fans and a manual roof peak vent. This green-
house maintained smaller temperature variations than the Brace house, which
responded to ambient temperature and light conditions faster. Due to the
nature of the Ken Post complex, this greenhouse was restricted to "clean
solution" work (nutrient solutions devoid of carbon sources, which cause
obnoxious odors).
Over the course of the project many different units were used in the Ken
Post greenhouse. For plant adaptability and culturing work a series of 20
small units fed from a common reservoir were assembled. Figure 6.12 is a
view of the system in operation. Each unit, measuring 22.8 cm wide by 3 m
long with 7.6 cm sides (9 in. x 10 ft. x 3 in.), was lined with plastic.
Five units together shared a common plywood base and were mounted on a large
greenhouse bench (see Figure 6.13). The units were sloped approximately
1 percent. A centrifugal magnetic pump supplied a fertilizer-based nutrient
solution to plastic pipe manifolds from which smaller tubes delivered solu-
tion to the individual units. Effluent from the units was collected in
gutters and returned to the reservoir. Thirteen centimeter (5 inch) mesh
wire screen was suspended 30 centimeters above the units to support plants
as they grew. Various root shading materials (black plastic, white plastic,
cheesecloth) were used to protect roots from clogging with algae.
A bench scale test system was built to test plant material production,
nutrient uptake, evapotranspiration and contaminant removal. A schematic
diagram of a single unit in this system is shown in Figure 6.14, and a
picture of the system in operation is shown in Figure 6.15. The system
consisted of six 10 cm (4 inch) by 1.5 m (5 foot) gutters with end pieces
and tube connectors on both ends. The gutters were sloped at 1 percent.
Each gutter section was connected to a solution reservoir by small tubes,
and a master flex tubing pump (Cole Parmer 7545-00) circulated solution
through each section. An additional water reservoir was suspended above the
solution reservoir. This water maintained a constant volume in the solution
reservoir by replacing water lost by evapotranspiration and thereby allowed
measurement of this ET.
The remainder of the glasshouse space was filled with several other units
over the course of the project. Large pilot scale units, smaller lengths of
.the same type unit, and high rate gross pollutant treatment systems were
located in the glasshouse.
For growing and transporting plants to the Hanover greenhouse, 0.76 x 6.1
meter (2.5 x 20 foot) units were built. Crosspieces of 5 x 10 cm (2 x 4
inch) lumber connected 6.1 meter (20 foot) long, 5 x 15 cm (2 x 6 inch)
86

-------
Figure 6.12. Post House 3 meter (10 ft) units in
operation.
iVWuiiS'
Figure 6.13. Construction of 3 meter (10 ft) units
in Post House.
87

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rt
SCREEN
PLANT MATERIAL
Z
Ji

J
GUTTER
FLOW
MAKEUP
WATER
RESERVOIR
x
ii
NUTRIENT-
SOLUTIONz
RESERVOIR
FLOW
PUMP
r'igure o
.li-. Schematic of Pest House 1.5 meter (5 ft) unit,
Figure 6.15. Pest House 1.5 meter (5 ft) 'unit in operation.
58

-------
stringers, as shown in Figure 6.16. A 1.3 cm (half inch) plywood floor was
placed on Che crosspiece and stringers, and plywood sides were attached to
the stringers. Legs of pressure-treated 10 x 10 cm (4 x 4 inch) lumber cut
at various lengths were bolted into the corners of the units. As in the
reconstructed unit in the Brace house, these units contained no cascades for
reaeration. The floor was made of three abutting sheets of plywood.
A.3.b. Loading Rates Schedule and Plant Species Tested—
Nine experimental runs were completed in the bench scale units at Ken Post
laboratory. These experiments ran from November 1980 through July 1981 and
are described below and summarized in Table 6.5. Some of the experiments
were performed in parallel with the bench scale unit in Hanover, New
Hampshire.
Experiments 1 through 4 were performed to determine the rates at which
plants took up nitrogen, phosphorus and cadmium from solution. Various
plants were tested on synthetic wastewater without the carbon source to
define the nutrient uptake rates and evapotranspiration water use. These
recirculating units were sampled intensively (four times) the first day,
once each of the following days for a week, and every other day thereafter.
These experiments ran approximately two weeks. In these and subsequent
experiments, a gutter receiving flow but containing no plant materials was
used as a control. This control allowed comparisons of evaporation and
transpiration by different plants.
Experiment 5 was similar to earlier experiments except that the carbon
source was introduced and analyses included chemical oxygen demand and
solids. All species in this experiment except cucumbers were grown on
wastewater in the Hanover greenhouse before being placed in the units.
Experiments 6 and 8 tested the effects of salt concentration on evapotrans-
piration and nutrient uptake. In experiment 6 the salt used was an equi-
molar mixture of NaCl and CaCl2> and included concentrations of 0, 15, 30,
60 and 120 milliequivalents per liter. The largest of these is greater than
that of any irrigation water used in the arid west. All units except the
control contained reed canary grass, which, being a grass, is tolerant to
salts relative to other test species. In this experiment the calcium (Ca)
tended to precipitate with the ammonia and phosphate, making them unavail-
able to the plant and lowering the salt content. To remedy this, experiment
8 used NaCl only, with other conditions remaining the same. The sampling
schedule and duration of these experiments were the same as earlier experi-
ments.
Experiments 7 and 9 were simulations of various loading rates. This allowed
for testing of high loading rates not tested in the pilot scale units and
simultaneous testing of loading rates under identical light and temperature
conditions. Canary grass filled five units, and the sixth was an empty
control. Flow rates included 200, 160, 75, 42 and 21 per minute, which
corresponded to area loading rates of 182.9, 147.3, 68.6, 38.1 and 20.3
centimeters (72, 58, 27, 15 and 8 inches) per day, respectively. In these
89

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1C x 10 cm Leg
(V' x h")
Plwood Floor
_ x 40 en Plywood Side
(1/2" x 15")
Figure 6.l6. Superstructure (above) and plyvooa covered floor
(below*) schematics of 5-1 (20 foo~ ) portable
units.

-------
experiments, Che apparatus was operated as a once-through system, with the
effluent being wasted and the synthetic wastewater being made fresh every
12 hours. These units were allowed to come to equilibrium over a period of
a week, which included two complete analyses of the effluent. Then the
units were tested for 3 or 4 consecutive days to define their steady state
characteristics. Analyses included nutrients plus COD, BOD and solids.
TABLE 6.5. CORNELL BENCH SCALE EXPERIMENTS CONDUCTED IN THE GLASS COVERED
GREENHOUSE (KEN POST LABORATORY)
Experi- Starting Additional Amendments	Recycle
ment No. Date to Nutrient Solution	Test Species	or 1 Pass
1
11- 9-80
None
Soft rush, phragmites
cattail, canary grass
Recycle
2
1-19-81
None
Phragmites, canary
grass, woolgrass,
soft rush, bristly
sedge, control
Recycle
3
2-27-81
None
Millet, bristly sedge,
canary grass, soft
rush, napier grass,
control
Recycle
4
3-18-81
None
Cucumber, phragmites,
canary grass, bulrush
cattail, control
Recycle
5
4-28-81
Carbon source
Cucumber, Canary grass,
Bulrush, Cattail,
Phragmites, control
Recycle
6
5-13-81
Salts
Canary grass, control
Recycle
7
6-11-81
Carbon source
Canary grass, control
One pass
8
7- 2-81
Salt
Canary grass, control
Recycle
9
7-17-81
Carbon source
Canary grass, control
One pass
A.4. Analytical Methods
In the analysis of 12.2 meter canary grass units, one liter of sample was
collected at the distribution box, Point A, Point B, and above the effluent
barrel (for locations see Figure 6.2). At high flow rates, much settled
matter was flushed into the sampling ports immediately after their opening,
91

-------
and this necessitated waiting for the establishment of a uniform flow before
taking the sample. At each point, samples from each port were shaken and
composited to obtain a representative sample. For the analysis of the pilot
36.6 meter (120 foot) units, one liter samples were collected at the end of
each 12.2 meter (40 foot) unit. Again, samples were mixed and composited.
During intermittent operation, samples were taken during times of uniform
high flow. From the bench scale unit 75 or 200 m£ samples were taken,
depending on whether the analyses included COD and solids, or nutrients and
Cd only.
All gravimetric and chemical analyses were carried out in accordance with
Standard Methods for the Examination of Water and Wastewater (APHA, et al.,
1976), with the exception of the Chemical Oxygen Demand and Total Nitrogen
analyses, which will be described later.
Filterable residue was determined by passing 500 mi of sample through glass
fiber filters in Gooch crucibles. The filtrate, along with unfiltered
samples, was used in subsequent analyses. Total residue was determined by
adding 100 m£ of samples to crucibles. All crucibles were dried overnight
at 103°C. Following weighing, crucibles were ashed at 550°C for one hour to
determine fixed and volatile fractions of the residue.
Nitrogen species were determined by distillation and titration, using 50 or
100 mt sample volumes. Ammonia was determined by the preliminary distil-
lation method using phosphate buffer. Nitrate was determined on the same
sample after the addition of Devarda's alloy for nitrate reduction. Organic
nitrogen was determined by the Kjeldahl method. Values for urea were
obtained by incubating samples and blanks with urease enzyme (AOAC, 1960)
and then distilling urea plus ammonia.
To determine total N on a wastewater sample (other than totaling N species)
the procedure of Raveh and Avnimelech (1979) was used. The procedure uses a
potassium persulfate oxidation in an autoclave, followed by reduction of
nitrates and nitrites to ammonium with Devarda's alloy. Ammonia nitrogen
was then determined by distillation.
Orthophosphate was determined on appropriately diluted samples by the
stannous chloride method, using a Bausch & Lomb Spectronic 70 spectrometer.
The azide modification of the iodometric method was used to determine
dissolved oxygen content.
The pH was measured on a Beckman Zeromatic SS-3 pH meter.
For cadmium, samples were preserved in 1% nitric acid and analyzed on a
Perkin Elmer Model 305 atomic absorption spectrophotometer.
To determine the chemical oxygen demand, the colorimetric method described
by Knechtel (1978) was used. Sample and reagent volumes proportional to,
but smaller than, those used in Standard Methods were added to Kimax 25 x
150 mm culture tubes with teflon-lined caps. Tubes were then inverted
several times for mixing, placed in a 150°C oven for two hours and cooled in
92

-------
a water bath. The appearance of Cr III In standards, blanks, and samples
was determined by absorbance readings at 600 ran on the Spectronic 70
spectrometer. Culture tubes were placed directly in the Spectronic 70 after
cooling to room temperature.
For plant tissue cadmium content, foliar or root samples of 0.50 or 0.75 g
were mixed with 5 mil concentrated HNO3 and stored overnight. They were then
placed on a microKjeldahl apparatus and heated until the fuming subsided and
solution cleared. Samples were then filtered, brought to a known volume,
and analyzed for cadmium in the same manner as water samples.
Hydraulic retention time (HRT) of the test unit was compared to control
units which received no wastewater flow. A chloride spike was used to
determine the hydraulic retention time of the test and control units under
various operating conditions. Ten grams of NaCl, dissolved in one liter of
water, were added where the water entered the grass, and periodic samples
were taken at the sampling ports and effluent stream. Chloride (Cl~)
analysis was conducted using an Orion specific Cl~ electrode and model
407A Ionalizer meter. In intermittently operated systems, hydrograph data
were used to correct for the effluent flow irregularity. Hydraulic
retention time was calculated using the formula:
HRT
where	t^
Ci
Ati
a Cj Att	(6>1)
Ci
¦ time of sample
=» Cl~ concentration, and
= sampling interval
B. PROCESS DEFINITION WITH SEWAGE - STUDIES AT HANOVER, NEW HAMPSHIRE AND
AND CAYUGA HEIGHTS, NEW YORK
B.l. Pilot Scale Units
B.l.a. CRREL Greenhouse and Test Units—
The NFT model used in the CRREL study was a plywood tray 0.61 m (2 ft.) wide
and 12.2 m (40 ft.) long lined with plastic and Installed at a 5 percent
slope. Due to space restrictions in the greenhouse, two troughs 6.1 m (20
ft.) long were installed beside each other. The effluent from the first
trough flowed by gravity to the high end of the second trough which sloped
in the opposite direction. Figures 6.17 and 6.18 show the system as
Installed in the greenhouse, and Figure 6.19 is a schematic of the system
showing its major components. As shown in Figure 6.19, the rate of flow
onto the system was controlled by adjusting the flow control valve. The
length of the wastewater applications and resting periods was adjusted using
93

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Figure 6.17. Experimental IIFT unit for primary settled domestic sewage
studies at Cornell.
JlTfc7f*TWi




Figure 6.l8. View of initial placement of reed canary grass washed sod
^ust after the first harvest at CRRZL.
91+

-------
PRIMARY
SETTLING
STORAGE
TANK
vn
FROM MUNICIPAL SEWER
TIMER
FLOW METER
SOLENOID
NrT TROUGHS
LOW CONTROL
VALVE
TAPWATER CONN.
FLOW METER
TO MUNICIPAL SEWER
oclu.'inul. i c d.i ;.i(!;r;ii[i of' I.h;: f-HH I'M. Nh'T syst.ein.

-------
a timer capable of allowing an application period of zero to 30 minutes and
a rest period of up to 60 minutes. The two meters in Figure 6.19 were used
to measure total flows in and out of the system. The meter in the influent
line was also used to measure the instantaneous flow rate onto the system
during any particular application cycle. This capability was used in con-
junction with the flow control valve to adjust the amount of wastewater
applied per cycle and therefore the amount of wastewater applied each day.
The system also included the capability of applying tap water in the event
of mechanical failure of the wastewater delivery and application system.
The crop used in this study was reed canary grass sod that had the soil
washed from the roots prior to being placed in the model.
B.l.b. CRREL Loading Rates Schedule—
Table 6.6 summarizes the testing program for the sewage treatment study in
Phase 0. Due to the lack of information on the use of the NFT to treat
wastewater, the investigators relied upon their knowledge of the overland
flow method of wastewater treatment, thereby deciding initially to use
application rates of 10.2, 20.3, and 40.6 centimeters (4, 8, and 16 inches)
per day of wastewater. These rates gave volumes of 757, 1514, and 3028
liters (200, 400, and 800 gallons) per day, respectively.
In an effort to maintain aerobic conditions in the root zone, an intermit-
tent application schedule was used. The investigators initially decided to
use a 24 hr/day schedule consisting of 3.14 liters (0.83 gallons) per minute
for 10 minutes followed by a 50-minute rest period. This was followed by a
schedule consisting of 1.59 liters (0.42 gallons) per minute continuously
for 8 hours followed by a 16-hour rest period. The next schedule consisted
of 3.14 liters (0.83 gallons) per minute for 20 minutes followed by a 40-
minute rest period, for 12 consecutive hours. The system then rested for
12 hours. The above schedules were all intended to apply 10.2 centimeters
(4 inches) per day. Subsequently, an application rate of 3.14 liters (0.83
gallons) per minute for 10 minutes followed by a 50-minute rest period for
12 consecutive hours followed by a 12-hour rest period was implemented.
This was intended to apply 5.1 centimeters (2 inches) per day. Finally, in
order to apply 20.3 centimeters (8 inches) per day, an appliication schedule
of 4.16 liters (1.1 gallons) per minute for 20 minutes followed by a 40-
minute rest period for 18 consecutive hours was used.
At the beginning of Phase 1, CRREL continued testing reed canary grass in
the 12.2 meter (40 foot) unit. Table 6.7 shows the specific conditions
tested during this period. The conditions were similar to those tested in
Phase 0, but allowed the grass and root system to become acclimated to the
wastewater flow over a longer period of time than in Phase 0. The instan-
taneous flow rate onto the grass was identical in each case, and loading
rates were controlled by manipulating the length of the application period.
This unit was killed early in Phase 1, so the duration of experiments with
this setup is limited. The cause of death of the plants was traced to a
combination of operating conditions and a large spill of ammonia at CRREL.
96

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TABLE 6.6. SUMMARY OP CRREL STUDY ACTIVITIES IN THE 12.2 m PILOT SCALE
SYSTEM DURING PHASE 0
Dates	Activity
13	Nov 79 - 31 Dec 79	Troughs constructed and installed; plumbing
Installed, tested, and modified as required.
15	Nov 79	Reed canary grass placed in troughs.
16	Nov 79-2 Dec 79	Approximately 380 £/d of sewage or tap water
applied intermittently (0.62 m /m-d).
3 Dec 79 - 10 Dec 79	Hydraulic retention time studies conducted.
3 Dec 79 - 18 Dec 79	190 - 1890 £/d of wastewater or tap water
applied intermittently.
19 Dec 79 - 27 Dec 79	An average of 295 £/d of tap water applied,
10 min. on - 50 min. off, 24 hours per day
(0.48 m /m-d).
28 Dec 79 - 30 Dec 79	An average of 990 £/d of wastewater applied
intermittently.
31 Dec 79 - 11 Jan 80	Automated application of sewage, 10 min. on -
50 min. off, 24 hours per day, to give
1.25 m3/m-d (760 */d).
12 Jan 80 - 13 Jan 80	System rested.
14	Jan 80 - 18 Jan 80	Continuous application of sewage for 8 hours
followed by 16 hour rest period to give
1.25 m /m-d (760 1/d).
19 Jan 80 - 30 Jan 80	Automated application of sewage, 20 min. on -
40 min. off for 12 hours per day, rest 12 hours
per day, to give 1.25 m /m-d (760 I).
31 Jan 80 - 8 Feb 80	Automated application of sewage, 10 min. on -
50 min, off for 12 hours per day to give
0.62 m /m-d (380 *).
9 Feb 80 - 13 Feb 80	Automated application of sewage, 20 rain, on -
40 min. off for 18 hours per day, rest for 6
hours per day, to give 2.48 m /m-d (1510 £).
97

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TABLE 6.7. LOADING CONDITIONS TESTED IN THE 12.2 tn PILOT SCALE SYSTEM IN THE
CREEL GREENHOUSE DURING PHASE 1
Loading Rate	Application Period
Dates	(cm/day) (liters/day) (m /m-d) (minutes per hour)
15
Feb
80 - 11
Apr
80
5.1
379
0.62
12
Apr
80 - 13
June
80
10.2
757
1.24
14
June
80 - 30
June
80*
20.3
1514
2.48
*Unit terminated due to death of plants shortly after this date.
B.l.c. Hanover Greenhouse and Test Units—
In the summer of 1980 larger systems were constructed to expand the scope of
the study. It appeared that the desired treatment from the hypothesized
three-part system (rough filtering, nutrient removal and polishing) was not
realized from the 12.2 or 24.4 meter (40 or 80 foot) units. In the Cornell
Brace house, 12.2 meter (40 foot) units would be connected to provide a
sufficiently long system for synthetic sewage testing, and a new temporary
greenhouse structure would be erected at a sewage treatment plant.
The Town of Hanover, New Hampshire, sewage treatment plant was selected
because it offered the following advantages:
1.	a medium to low strength domestic sewage with few industrial
sources;
2.	a site conducive to greenhouse location;
3.	city officials and operators interested and willing to cooperate;
4.	close proximity to CRREL, which could perform analyses.
In mid-November 1980 a subcontract was executed between Cornell and the Town
of Hanover; and a Cornell crew, aided by local contractors and the sewage
plant personnel, began construction. The greenhouse was sited on the
south-facing side of the treatment plant building and attached digester.
The site sloped downward to the west, necessitating a retaining wall and
backfill to level the site. The location of the greenhouse in relation to
the plant is shown in Figure 6.20.
The greenhouse choice was based on minimum cost and ease of construction.
The design chosen was the "Twenty-One" style wooden gusset greenhouse
invented and described by Sheldrake and Sayles (1977). This house is
constructed from simple trusses strenthened with external plywood gussets
and covered with two layers of plastic attached from the outside. Gusset
houses are fairly simple to erect and can be constructed by relatively
98

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SEPTAGE
TANK
CLARIFIER
COM''1T SCREENS
North
CLARIFIER
PLANT BUILDING
DIGESTER
GREENHOUSE
GRIT
TANKS
Figure 6.20. Si^e plan of greenhouse ar.d Hanover,
Hampshire, sewage treatment plant.
New
99

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unskilled laborers using standard stock lumber. One complicating feature
of the Hanover site was the necessity of building the greenhouse around the
curved surface of the digester wall. The trusses, which were attached to
posts on 1.2 meter (4 foot) centers, were made of unequal length around the
digester, and the small area of roof above this curved section was covered
with a solid rather than plastic outer covering. Figure 6.21 shows the
construction of unequal trusses around the digester. The completed green-
house is shown in Figure 6.22.
The greenhouse was 18.3 meters (60 feet) long and 9.1 meters (30 feet) wide
overall. However, the digester took up almost one-fourth of this space,
which resulted in a usable floor area of approximately 130 square meters
(1400 square feet). This was adequate space to construct two 36.6 meter
(120 foot) systems. The first unit was built to parallel the 36.6 meter
(120 foot) system constructed in the Cornell Brace house, and was con-
structed from portable 6.1 meter (20 foot) sections described earlier and
illustrated in Figure 6.16. Six of these sections were joined to make up
three 12.2 meter (40 foot) lengths and connected similarly to the Brace
house system. A second set of six 6.1 meter (20 foot) sections was joined
by gutters to form a single gravity fed unit. The arrangement of trays,
direction of flow of wastewater, and associated equipment are shown schema-
tically in Figure 6.23. The interior of the greenhouse and trays under
construction are shown in Figure 6.24.
Due to problems experienced with the chlorination practice of the treatment
facility (chlorine was added upstream from the primary clarifier), a small
primary sedimentation tank was constructed. It consisted of modifying a
little used pump out chamber located at the head works of the plant. A
small portion of the raw sewage entering the treatment plant was diverted to
a2.1mx2.1m(7x7 foot) pumpout chamber, which acted as a primary
clarifier. This sewage, which resembled primary settled sewage, was then
used in the greenhouse. The tank had an upper overflow pipe to allow a
continuous supply of fresh sewage to be pumped into the greenhouse and a
lower waste line to remove solids settling in the tank. A 0.19 kilowatt
(1/4 horsepower) pump agitated the tank contents and was capable of deliver-
ing any desired flow. In the main testing system (shown as unit A in Figure
6.23), primary settled wastewater was pumped to the supply tank and then
pumped onto the first 12.2 meter (40 foot) unit as needed. At the end of
the first level 12.2 meter (40 foot) unit, the sewage was pumped up to the
second unit from a catch barrel. At the end of the first testing condition,
the first unit was raised so that wastewater flowed by gravity to the next
unit. This unit was sloped at 2% and wasted directly into the third, which
was also sloped. (See Figure 6.10 for a description of the identical Brace
house system.)
In the second 36.6 meter (120 foot) system, sewage flowed from the supply
tank to a reservoir below the first unit. It was then pumped up to the
first tray from which it flowed by gravity through each subsequent tray.
A desk and laboratory work space were installed in the southwest corner of
the greenhouse. This space was used for BOD analysis, microscopic work,
100

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Figure 6.21. Attachment of trusses to pos^s during
construction of the Hanover greenhouse
Trusses were built of unequal lengths
to fit around the digester wall.
Figure 6.22. View of the plastic covered Hanover
greenhouse, treatment plant building,
and surrounding grounds.
101

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9.8 m
CATCHv BARREL
UNIT B4
FLOW
HANOVER GREENHOUSE
| UNIT B3 FLOV%
UNIT
B 5
UNIT
B 6
4.6 m
PUMP-a
FLOW
FLOW
UNIT B2
FLOW
/"FEED RESERVOIR
4) UNIT BI FLOW
SUPPLY
8.5 m
SUPPLY
i TANK
CATCH a STORAGE RESERVOIR
i ¦
DRAIN
9.2m
UNIT A I
^ FLQW
CATCH BARREL
SLUDGE UJ
waste fry
DRAIN x
A SUPPLY
'I OVERFLOW
FLOW
UNIT A 2
4.6 m
UNIT A3
18.3m
L-e 6.?3. Schematic diagram of unil.c in Lhe Hanover greenhouse.

-------

Figure 6.2k. The inferior of the Hanover greenhouse,
treatment plant building, and surrounding
grounds.
103

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plant preparation and manipulation, and equipment storage. The bench scale
unit was placed in a small area near the digester wall.
B.l.d. Hanover Loading Rates Schedule and Plant Species Tested—
Over the course of spring of 1981 several loading conditions were tested
in the Hanover greenhouse pilot unit. These conditions are summarized in
Table 6.8. Several changes made over the course of this testing period
should be noted. As in the Cornell Brace house system, the cucumbers did
not perform well in the Initial loading condition, and plant species were
rearranged following this first testing period. The first 12.2 meter unit
had a small slope, and this was not removed until the third condition.
Toward the end of the second condition and throughout the third condition,
the reed canary grass did not perform well and began to die. This was In
contrast to the Cornell Brace house canary grass which appeared healthy. In
an attempt to rejuvenate the grass, the system was not pushed to the 40.6
centimeter (16 inch) per day loading as was the Cornell unit, but rather the
system was dropped back to a 5.1 centimeter (2 inch) per day loading. The
reed canary grass eventually recovered under this low loading. Experiments
in the Hanover facility were terminated in August, 1981. The facility was
converted to a storage area for the treatment plant in October, 1981.
TABLE 6.8 LOADING CONDITIONS TESTED IN THE PILOT SCALE UNIT
AT THE HANOVER TREATMENT PLANT
Dates
(cm/day)
Loading Rate
(liters/day)
(m3/m-d)
Plant
Species
Application
Mode
2/13/81-
3/20/81
6.9
1893
2.49
Phragmites
Cucumbers
Reed canary grass
Continuous
4/13/81-
5/ 8/81
10.2
2839
3.74
Cattail/bulrush
Phragmites
Reed canary grass
Intermittent
15 minutes
application
15 min. rest
5/9/81-
6/3/81
20.3
5678
7.47
Cattail/bulrush
Phragmites
Reed canary grass
Intermittent
30 minutes
application
30 min. rest
6/13/81-
8/21/81
5.1
1420
1.87
Cattail/bulrush
Phragmites
Reed canary grass
Intermittent
30 minutes
application
30 min. rest
Rest overnight
104

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B.l.e. Root Analysis
Root sampling devices similar to those used at Cornell were installed in
Unit 1 at the Hanover greenhouse. The sampling procedure and frequency was
identical to that performed at Cornell, described in a previous section.
Root sampling baskets were extracted both at the midpoint and at the end of
each loading condition.
Periodically, a qualitative description of the plants and root systems in
Unit 2 was recorded. Unit 2 contained a variety of species, constantly
receiving sewage at a rate of 6.9 cm/day throughout the study. Information
collected on Unit 2 included: plant height, coloring and condition; esti-
mated leaf surface area (when applicable); appearance of accumulated algae
and other growth in the water and under the plants; and description of
material entrapped by the root mass. The measurements and descriptions of
the root mass included: approximate average root length and diameter; the
approximate density and arrangement of the roots; apparent new growth; root
mat thickness; and any irregularities in color, texture and uniformity.
Observations were documented by photographs whenever possible.
B.l.f. CRREL Analytical Procedures—
Grab samples of the applied wastewater and of the runoff were delivered to
the water chemistry and environmental engineering laboratories at CRREL
where they were analyzed for nitrogen species, orthophosphate, total organic
carbon, solids, turbidity, pH, biochemical oxygen demand, and fecal coliform
bacteria, in accordance with Standard Methods (APHA et al., 1976). Total
nitrogen analyses were performed by the same persulfate digestion procedure
used at Cornell, which was described earlier. In addition, CRREL performed
total phosphorus analyses on an aliquot of sample removed from the total
nitrogen digest before Devarda's metal addition. Both volatile and nonvola-
tile trace organic determinations were conducted by CRREL personnel using
a gas chromatograph - mass spectrometer combination. Not all samples were
analyzed for all these parameters. Hydraulic retention time determinations
were identical to the procedures used at Cornell. Samples for Cd analysis
were shipped to Cornell. Grass was harvested periodically, and fresh and
dry weights were obtained. A subsample of grass from the last harvest was
sent to Cornell or another laboratory for tissue analysis. Daily, maximum,
and minimum temperatures in the greenhouse were recorded, as were incoming
solar radiation measurements.
B.2. Bench Scale Units
B.2.a. Hanover Bench Scale Units and Experiment' Schedule—
A 1.5 meter (5 foot) bench scale system identical to the one in the Ken Post
greenhouse was set up to test plant material production, nutrient uptake,
carbon and cadmium removal, and evapotranspiration. Four of the plant
species tested came from the main unit. An additional species was taken
from the culturing unit to make a total of five species and a control in
each run. All bench scale systems were fed sewage. Sewage was obtained by
105

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dipping a small bucket below the surface of the 1893 liter (500 gallon)
reservoir and allowing it to fill. Seven and one-half liters were measured
into each of the six reservoirs and circulated through the trays at a rate
of 200 mA/min. Stirrers were used to completely mix reservoirs during the
first run only.
Four experimental runs, each lasting for a two-week period, were completed.
These experiments ran from March 1981 to July 1981 and are described below
and summarized in Table 6.9. The objective of the bench scale experiments
was to determine the correlation between removal efficiency and loading rate
by using plants acclimated to the various loading rates tested in Unit 1.
TABLE 6.9. HANOVER GREENHOUSE BENCH SCALE EXPERIMENTS
Experi- Starting Analysis	Acclimated Source of
ment No. Date, 1981 Run	Test Species Loading Rate Plant Material
1
3/23
N, P
Cucumbers


Unit
1


COD
Reed Canary






Solids
Grass (RCG)


Unit
1


ET
Phragmites
6.9
cm/day
Unit
1



Bulrush


Unit
2



Cattails


Unit
2



Control




2
4/17
N, P
Roses
6.9
cm/day
Unit
2


COD
RCG
6.9
cm/day
Unit
1


BOD
Phragmites
6.9
cm/day
Unit
1


Solids
Bulrush
6.9
cm/day
Unit
1


ET
Cattails
6.9
cm/day
Unit
1



Control
6.9
cm/day


3
5/ 7
N, P
Bulrush
10.2
cm/day
Unit
1


COD
RCG
10.2
cm/day
Unit
1


BOD
Phragmites
10.2
cm/day
Unit
1


Solids
Cattails
10.2
cm/day
Unit
1


ET
Napier grass
6.9
cm/day
Unit
2



Control




4
5/21
N, P
Tomatoes
6.9
cm/day
Unit
2


COD
RCG
20.3
cm/day
Unit
1


BOD
Bulrush
20.3
cm/day
Unit
1


Solids
Phragmites
20.3
cm/day
Unit
1


ET
Cattail
20.3
cm/day
Unit
1



Control




5
7/ 1
N, COD
RCG
20.3
cm/day
Unit
1
BOD
Sol ids
106

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The units were sampled every four hours for Che first day, twice the 9econd
day (12-hour intervals), once each of the next 5 days, and every other day
for the remaining 7 days. BOD's were run on site. Solids samples were
cooled, and COD and N samples were acidified to pH<2 using conc. H2SO4 (36N)
and shipped to Cornell for analysis. Evapotranspiration readings were taken
twice daily. In addition to the daily sampling, all species and the control
were drained and weighed before and after each experimental run to determine
the increase in biomass during the experimental period.
Experiment 5 evaluated BOD removal efficiency using reed canary grass at
various loading rates. The apparatus and operating procedure were identical
to that of experiments 7 and 9 performed in the Ken Post greenhouse at
Cornell. Masterflex positive displacement pumps fed sewage to the unit in a
one-pass flow scheme from an 1893 liter (500 gallon) reservoir. The experi-
ment ran for 13 days. Samples were taken on days 1, 3, 7, 11, 12 and 13 and
were handled in the same manner as they were for the previous four experi-
mental runs. On the day of sampling, volumes retained in each tray were
measured after sampling. Pumps were shut down and trays were allowed to
drain for two hours. The volume of sewage collected was measured as an
indicator of the HRT of each tray.
B.2.b. Cayuga Heights Greenhouse, Bench Scale Units and Experiment
Schedule—
From clarifier design parameters, it was evident that the NFT systems
designed and used in early experiments were not optimal for removing
suspended solids. This had two detrimental effects on the system—first,
a long length of unit was necessary to perform the required treatment, and
second, the solids which passed through the first part of the system could
inhibit the more valuable but delicate plants in downstream portions. The
typical tray design did not handle solids well because at higher loading
rates the velocity in the thin films was too great to allow for sedimen-
tation. An earlier attempt to improve solids handling was to remove the
slope from the first unit in three-unit systems. While leveling the unit,
in combination with using plants with large roots, did improve solids
removal, its performance was still below expectations. For these reasons
a new system was designed consisting of sequentially filled chambers where
root zones could be alternately flooded with wastewater and drawn down for
aeration and drying. In this way the sedimentation process could be opti-
mized and the root zone could provide a filter for entrappment of solids
and a foundation for microbial attack. By raising and lowering the
wastewater level slowly, flushing of the root zone and trapped material
would be minimized until such time as solids removal from the unit was
desired.
The purpose of these experiments was threefold. The first purpose was to
find out whether a system with a high flow capacity would be capable of
performing a large portion of the solids removal (settling and/or metabo-
lism). Could a system be designed which would contain the sewage long
enough for significant treatment and minimize solids flushing when the batch
of wastewater was changed? The second purpose was to see which plants could
107

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tolerate submersion in a fairly deep (30 cm) pool of standing wastewater
with periodic drying. The third purpose was to investigate the role of this
type of unit in an overall treatment scheme. Significant improvement over
earlier NFT designs in solids handling capability and organic removal rate
could lead to its use as a pretreatment step or as a possible replacement
for conventional primary and secondary treatment units.
Since the Cayuga Heights system was intended for summer operation only, a
minimal greenhouse cover was built. A single sheet of polyethylene was used
to cover a wooden frame of lean-to design enclosing an area of approximately
3 x 6 m. The enclosure had a southern exposure, and abutted an anaerobic
digester and associated building, which provided protection from north and
northwest winds. The enclosure provided protection from early frosts and
rain. Openings were provided at both ends so air could move freely through
the greenhouse. The greenhouse operated successfully with neither heater
nor forced draft ventilation until electric heaters were added for night use
during the last two weeks of the test period.
The test units were 30 x 122 cm (1 x 4 ft) fiberglass boxes with 40 cm
sides. A baffle plate was placed 10 cm from the end of the box. An exit
port was placed between the baffle and end; sewage was introduced through a
hose placed in the opposite end in plant material. A schematic diagram of
the unit is shown in Figure 6.25. The unit sides were opaque, but plant
material did not appear inhibited by lack of light since the plants extended
well above the units. Figure 6.26 shows napier grass about 2 m tall
extending from the unit.
Three units were connected in series to form a system. Screened raw
wastewater was pumped continuously into the first unit by a Masterflex
positive displacement pump. Influent was obtained from a manhole
immediately upstream of the primary clarifiers. Turbulence in the manhole
insured a completely mixed, representative sample of raw influent. To guard
against clogging the small diameter tubing with grass solids, a basket of
window screening was placed around the inlet before lowering into the
manhole. Thus the liquid level was allowed to rise in the first unit over a
four-hour period, after which it was pumped out from the exit port and into
the next unit. The second and third units were operated in a batch mode,
the effluent from the second unit entering the third and the effluent of the
third unit pumped to the primary clarifiers of the treatment plant.
Centrifugal pumps were used to transfer the flow between units and dispose
of effluent. These pumps were controlled by three independent 24-hour
timers which were operated synchronously. Ball valves were used to control
the pumping rate between units to effect a smooth transfer of the contents
within the 15 minute "in" cycle allowed by the timers. A schematic diagram
of the system is shown in Figure 6.27. The time cycle of operation is noted
on this figure. A picture of the shelter and enclosed units is shown in
Figure 6.28.
Sewage was sampled as it entered the first unit and as it left the third
unit. A proportional sampler was devised to collect a continuous sample
from the effluent (shown in Figure 6.28). Although the pumping system
devised for this experiment worked accurately and reliably, it was a
108

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En-cry Hose
122 cm
Baffle Plat;
^-C cm
t.
Zxi^ Hose
Figure 6.2> . Test unit used in the high
loading rate experiments using
domestic sewage at "he Cayuga
Heights treatment plant.
109

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Figure 6.26. Napier grass used in high loading
experiments at the Cayuga Heights
treatment plan:,.
110

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liters Siphon to


Timer
Timer
evage
Exit
Pump
rump
15 min
Flow
Flow
Third Unix
econd Unix
First Unit
Filled 1 hr.
Empty 3 hr.
Filled 2 hr.
Empty 2 hr.
15 ^in
flow
¦^attails 9
Soft F>ush
Napier
Grass
sewage
Entry
figure 6.2?.
Schematic diagram cf experimental
for high loading rate experiments
Cavuga Heights treatment slant.
system
it the
;ea
111

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Figure 6.28. Plastic shelter and enclosed units at the
Cayuga Heights, New York, treatment plant.
112

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compromise between the ideal of moving wastewater instantly and without
disturbance from tank to tank, and the reality of working within the
constraints of time with readily available materials. The use of high speed
centrifugal pumps did produce some disturbance within each unit and may have
caused some change in solids properties. It is felt that a design which
could avoid rapid handling of the wastewater at any point in the system
would provide for intermediate sampling and a more realistic view of actual
system capabilities. Such a system might employ timer-actuated gates or
valves and gravity flow. Plants for these experiments were chosen from
among those that appeared to thrive in a sewage environment (as demonstrated
in experiments at the Hanover greenhouse). The first unit was filled with a
mixture of cattail and soft rush, the second with bulrush and the third with
napier grass. All plants were cut to a height of 30-40 cm above the roots
and transplanted from Cornell greenhouses to the site. They were not cut
again during the test period. Napier grass was placed in the last unit and
provided with the greatest proportion of aeration time (three hours out of
four) because previous experience had shown it to be susceptible to injury
due to waterlogging. The napier grass performed extremely well, however,
under these conditions. Bulrush was also well adapted and flourished under
the conditions of the test. The cattails did not show as vigorous a
response to the conditions. They could have been influenced by any number
of factors, including the sewage characteristics and the fluctuating water
level around their roots. Predation and disease were not a problem for any
of the plants in this experiment.
After start-up in mid-July, the system was operated for four weeks at a
loading of 20 cm/day. Loading was then increased to 30 cm/day for the
remaining eight weeks of the test. Loading rates are summarized in Table
6.10.
The system was tested for soluble and total COD; suspended, total and
settleable solids; and NHi/-N and TKN (organic N by difference).
TABLE 6.10. SCHEDULE OF EXPERIMENTAL CONDITIONS AT CAYUGA HEIGHTS
Dates
Area Loading
Weir Loading
Volume Wastewater
Daily
From To
Rate
Rate
Batch
Volume

cm/d
m /m-d
4/4 hr cycle
l/d
7/20/81- 8/14/81
20
0.74
37.7
226
8/15/81-10/11/81
30
1.12
56.7
340
B.3. Comparison
of Synthetic and
Actual Domestic
Wastewater

The synthetic wastewater used in this study was modeled after a medium
strength domestic wastewater. There are several differences between this
synthetic mixture and the wastewater on which it is modeled, and there are
113

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also other differences between the synthetic wastewater and the wastewater
used at CRREL and Hanover.
Section A.l describes the synthetic mixture used and lists the target con-
centrations of nutrients and contaminants. While it was possible to
approach the chemical composition of sewage, there remained physical and
biological differences between real and synthetic sewage. Essentially all
of the constituents in the synthetic wastewater were dissolved, with only
small amounts of suspended or colloidal material. In contrast medium
strength sewage contains about 250 mg/£• of suspended and colloidal solids
(Metcalf and Eddy, 1972). When solids were present in the synthetic sewage,
it was usually caused by either (1) an inorganic precipitate which formed at
high temperatures in the greenhouse, or (2) microbial matter produced in the
feed concentrate container. The latter was suspected of being yeast since
the feed acquired a yeast odor over the two or three days between new
batches, and yeast extract was used as an ingredient. These solids never
approached the concentrations of those in real sewage. With the exception
of the aforementioned yeast, the synthetic sewage was devoid of any signifi-
cant microbial population, which is in sharp contrast to real sewage. The
NFT units' operation, however, did develop large populations of micro-
organisms in the root zone.
Comparisons of influent synthetic and real sewages used in this study are
shown in Table 6.11. In comparison with the typical medium strength waste-
water on which the synthetic sewage was based, the domestic sewage had lower
concentrations of several constituents. Organics, phosphorus and solids in
Hanover and CRREL sewage were less than typical values for sewage; and
organics and phosphorus were less than those of synthetic sewage. Since the
synthetic sewage contained few solids, the relatively low solids values of
Hanover and CRREL were still much greater than those of the synthetic mix-
ture. There were only small differences between Hanover and CRREL sewages,
which is reasonable since CRREL draws its sewage supply from the Hanover
system. While the Hanover system treats wastes from the entire town, the
waste stream above CRREL includes only residential development plus inter-
mittent accidental additions from the CRREL laboratories. As described in
section 4.2.1, Hanover sewage was selected based on a number of factors
suitable to the study.
Total and soluble biochemical oxygen demand (BOD) values in sewage were half
of those in the synthetic substrate. Total and soluble chemical oxygen de-
mand (COD) values were also less, but were greater than half. Part of this
difference was due to the initial high target concentration of 400 mg/1 for
the synthetic wastewater COD. For Phase 0 and the first condition of Phase
1, total and soluble COD averaged 403 and 392 mg/ £> respectively, while for
the remainder of the study they averaged 249 and 236 mg/ I, respectively.
These latter values are closer to those found in CRREL and Hanover sewage.
Unlike domestic sewage, there was little difference between total and solu-
ble BOD or COD since both were derived from a completely soluble substrate
(sucrose). Small differences may reflect microbial growth in the feed.
Total nitrogen (N) concentrations were similar in the synthetic and real
wastewaters, although the forms were different. Domestic sewage contained
llU

-------
predominantly ammonium N (NHi++-N) whereas synthetic sewage N was added as
urea. In nearly all cases, however, this urea N was rapidly hydrolyzed to
NHit+-N. Real sewage rarely contained nitrate-nitrogen (N0 3~-N) and
synthetic sewage often had traces of N0 3~-N, but never more than a few
percent of total N.
Since phosphorus was added to the synthetic mixture as potassium phosphate
salts, orthophosphate phosphorus (PO^-P) represented total P.
TABLE 6.11. CHARACTERISTICS OF INFLUENT SYNTHETIC AND REAL SEWAGE
(All units are mg/ I except pH)
Synthetic Sewage	Real Sewage+
Variable	Mean Std. Dev.	Mean Std. Dev.
pH
6.9
0.3
7.3
0
COD
307
132
241
18
Soluble COD
288
98
175
11
BOD
186
26
103
29
Soluble BOD
169
23
80
5
TOC


101
10
Total N
35.7
8.9
36.9
10
Total Soluble N
35.4
9.3
	
—
Total P
	
	
6.9
1
Ortho P
13.8
3.4
5.7
2
Soluble Ortho P
13.5
3.1
	
—
Cd
0.08
0.04
	
—
TS
492
180

—
TVS
296
141


TSS
5
3
71
38
VSS
3
2
61
28
+Average values of CRREL and Hanover primary settled sewage.
Total P of synthetic sewage was about twice that of the real sewage and
greater than double the real sewage POi^-P.
115

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As noted earlier, Hanover and CRREL total and volatile suspended solids (TSS
and VSS) were lower than typical values for sewage, while TSS and VSS in the
synthetic sewage were virtually absent.
C. TESTING APPROACH
C.l. Parameter Evaluation
The primary procedure used in this project was to subject various plant
species, primarily reed canary grass, to different applications of sewage or
synthetic wastewater and measure both treatment efficiency and plant
response. Many different flow rates were used. In continuous application
systems, the flow rate is proportional to both the system^area and the
required area loading rate. For example, to load a 9.3 m system with 10 cm
of sewage per day requires a continuous application of 0.65 A/min; loading a
system with 20 cm/day or using a system of 18.6 m at 10 cm/day would
require twice the continuous application rate. These area loading rates are
the primary references to loading conditions examined in this project.
Intermittent applications of sewage were used when it became apparent that
long periods of submergence might damage the plants. To describe conditions
tested under these intermittent loadings, the primary reference remained the
equivalent depth of sewage over the system's area over a 24-hour period.
Thus, a loading of 10 cm/day represents the same volume of sewage when
applied to a single size system, regardless of application mode; for a
9.3 m system this is 936 If day. Intermittent applications are described by
the length of time sewage is applied and the length of resting period
between applications. The instantaneous flow rate during application
periods is then the flow rate of the continuous application divided by the
fraction of time sewage is actually applied. Another way to view this is
that the daily loading is condensed into a shorter application time, and
instantaneous flow rate is increased correspondingly.
C.2. Season, Temperature and Environmental Controls
Nutrient film pilot systems were operated year-round, and this presented
wide variations in greenhouse conditions. Both Ithaca and Hanover have
short and relatively dark, overcast days in winter, with very cold outside
air temperatures. In contrast summer days are long and warm, although
Ithaca in particular is often overcast. No auxiliary lighting was used to
lengthen photoperiod in the greenhouses. Electric heaters were used to
maintain night temperatures at a minimum of 15°C. In spring, summer, and
fall, ventilation fans were used to cool greenhouses when the temperature
exceeded 25°C, although temperatures of 37"C were attained on some hot,
sunny days.
Continuous temperature and humidity recorders were placed in each green-
house, and incident solar radiation was measured at the Cornell Atmospheric
Sciences Laboratory and at the CRREL weather station.
116

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C.3. Test Parameters and Duration
Over the course of pilot scale experiments the loading rate varied from 4 to
AO cm/d, and most conditions tested were in the vicinity of 10 cm/d. Tables
6.12 and 6.13 summarize the schedules of the pilot scale systems for syn-
thetic and domestic sewages, respectively. In addition to varying the dally
loading, the method of application (continuous or intermittent) was also
varied. Specific information on intermittent loadings is shown in sections
A.2.b., B.l.b and B.l.d. As the project progressed, larger (longer) systems
were used to estimate pollutant and nutrient removal over a range of root
zone conditions. This Increasing size culminated in the three-part systems
at both Cornell and Hanover developed in the late stages of the project.
Reed canary grass was the primary test species for first part of the
project, while in later stages a varied collection of plants was used.
TABLE 6.12. TEST PARAMETERS AND DURATION OF THE CORNELL PILOT SCALE SYSTEMS
USING SYNTHETIC SEWAGE
Daily	Flow Rate
Dates Loading Application During	System Plant
Start End ^atf Mode Application	Area Species
cm/d m /m-d &/m	m
12/ 6/79- 1/ 2/80
10
1.24
Continuous
0.66
9.3
Canary
grass
1/ 3/80- 1/27/80
10
1.24
Intermittent
2.6
9.3
Canary
grass
1/28/80- 2/20/80
10
1.24
Intermittent
5.2
9.3
Canary
grass
2/21/80- 3/17/80
10
1.24
Intermittent
2.6
9.3
Canary
grass
3/18/80- 5/11/80
5
0.62
Continuous
0.33
9.3
Canary
grass
5/12/80- 6/17/80
10
1.24
Continuous
0.66
9.3
Canary
grass
6/18/80- 7/ 3/80
5
0.62
Continuous
0.33
9.3
Canary
grass
7/ 4/80- 9/ 8/80
20
2.49
Continuous
1.3
9.3
Canary
grass
9/ 9/80-10/15/80
10
2.49
Continuous
1.3
18.6
Canary
grass
10/16/80-10/31/80
20
4.98
Continuous
2.6
18.6
Canary
grass
11/ 1/80- 2/ 6/81
15
3.74
Continuous
1.0
9.3
Canary
grass
2/13/81- 3/14/81
7
2.49
Continuous
1.3
27.9
Phragmites






Cucumber






Canary
grass
4/16/81- 5/10/81
10
3.74
Continuous
2.0
27.9
Cattail/bulrush






Phragmites






Canary
grass
5/11/81- 6/10/81
20
7.47
Intermittent
7.9
27.9
Canary
grass
6/11/81- 7/ 1/81
40
14.94
Intermittent
16
27.9
Canary
grass
7/ 2/81- 7/30/81
7
2.49
Continuous
1.3
27.9
Canary
grass
7/31/81- 8/21/81
10
3.74
Intermittent
3.9
27.9
Canary
grass
117

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TABLE 6.13. TEST PARAMETERS AND DURATION OF THE CRREL AND HANOVER PILOT
SCALE SYSTEMS USING DOMESTIC SEWAGE
Daily	Flow Rate
Dates	Loading Application During System Plant
Start	End	Rate	Mode Application Area Species


cm/d
m /m-d

l/m
i.
m


11/16/79-:
12/ 2/79
5
0.62
Intermittent
	
7.4
Canary
grass
12/19/79-:
12/27/79
4
0.48
Intermittent
1.2
7.4
Canary
grass
12/31/79-
1/11/80
10
1.25
Intermittent
3.2
7.4
Canary
grass
1/14/80-
1/18/80
10
1.25
Intermittent
1.6
7.4
Canary
grass
1/19/80-
1/30/80
10
1.25
Intermittent
3.2
7.4
Canary
grass
1/31/80-
2/ 8/80
5
0.62
Intermittent
3.2
7.4
Canary
grass
2/ 9/80-
2/13/80
20
2.48
Intermittent
4.2
7.4
Canary
grass
2/15/80-
4/11/80
5
0.62
Intermittent
3.2
7.4
Canary
grass
4/12/80-
6/13/80
10
1.24
Intermittent
3.2
7.4
Canary
grass
6/14/80-
6/30/80
20
2.48
Intermittent
3.2
7.4
Canary
grass
2/13/81-
3/20/81
7
2.48
Continuous
1.3
27.9
Phragmites
Cucumber
Canary grass
4/13/81-
5/ 8/81
10
3.74
Intermittent
3.9
27.9
Cattail/bulrush
Phragmites
Canary grass
5/ 9/81-
6/ 3/81
20
7.47
Intermittent
7.9
27.9
Canary
grass
6/13/81-
8/21/81
5
1.87
Intermittent
2.0
27.9
Canary
grass
C.4. Loading Rate Characterization—
There are several parameters that can be used to describe loading rates to
an NFT unit. Three of them are described here. They are the area-volume
parameter (the depth of liquid added per unit area per day, cm/m -d or
cm/d), the weir parameter (volume applied per unit width of system, m /m-d)
and the retention time parameter (hydraulic retention time, rain). The first
two can be strictly operator-controlled, while the third can be variable
depending on specific conditions present in the system and the length of the
unit.
118

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One way of describing a loading rate is to determine the equivalent depth of
wastewater applied to an area. For example, if a cubic meter of wastewater
is applied to an area of 50 square meters, the equivalent depth added is two
centimeters. When a time dimension is added, the loading regime is
completed. This loading rate parameter was originated in land application
work, where land areas were used in various capacities to treat wastewater.
As in land application systems, the methods of applying the wastewater and
the system configurations of NFT systems can vary widely.
The depth added parameter is independent of the velocity of wastewater
through the system. Velocity is determined by the rate at which wastewater
enters the system during operating cycles and the slope of the units
comprising the system.
From this parameter upper and lower limits to loading can be estimated. The
lower limit must be high enough that water can flow through the entire
system after taking evapotranspiration into account. Also, a low flow must
be capable of supplying sufficient nutrients to plants and associated growth
in the root zone. Generally, low flow rates will not be used in NFT systems
since their use increases the area requirements of the system. However,
they may be used to achieve efficient nutrient removals and thus a high
quality effluent. Upper limits can be defined by desired effluent quality,
with respect to BOD, SS and nutrients. Just as plants are limited in their
ability to take up nutrients, the root zone, with its associated organisms,
is limited in its ability to assimilate carbon and nutrients and to trap
solids. Nutrient uptake is generally proportional to the area, while SS
treatment depends on other factors (depth and velocity).
Another way of expressing loading rate to an NFT system is the loading per
unit width of the unit, typically having units of m /m-d. This parameter
which originated in overland flow land application describes the volume of
water crossing the weir boundary into the system. This parameter is
independent of the length of the units, unlike the area-based parameter
which is dependent on length and width. It is useful in design where the
length of a unit is fixed. Typical loading rates applied in overland flow
treatment are less than 1.0 m /m-d (USEPA, 1981).
Since many physical, chemical and biological pollutant control processes are
time dependent, it may be useful to describe loading rates in terms of
contact time of wastewater in the system. Hydraulic retention time (HRT)
analyses determine the amount of time water resides in the system. Given
the flow rate of water through a system, the volume of a system can be
calculated as
V = (HRT) (Q)	(6.2)
where	V = volume in i
HRT = retention time in min. and
Q = flow in tymin.
119

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Since NFT unit9 have open tops and flow is by gravity, nuch variation in
water depth, volume and residence time is possible due to differences in the
root zone condition.
Unlike the first two parameters described, HRT is not directly operator
controlled. Areal and weir rates are adjusted where wastewater enters the
system and can be controlled fairly precisely. Retention time can be
estimated from the water depth and flow rate, or from empirical experience,
but the exact determination requires experimentation. Although HRT may be
known exactly at some point in time, it may change over the course of an
experimental period due to changes in the root zone (clogging, flushing,
growth, etc.).
Throughout this report, loading rates are referred to by the area-depth
parameter because different size systems were used and this parameter was
easily calculated, unlike the HRT parameter.
Comparisons between the three loading rate parameters are shown in Table
6.14. The area and weir loading rates are proportional within each size of
unit tested. As the surface areas of the units are increased, the width is
held constant and the length is extended (as described in section
6.a.2.b.). Thus the area loading rate (and the retention time) can be held
constant, but the weir loading rate will increase for a longer unit. While
the area rates give a better indication of the volume of water applied to
the units, the weir rates give an indication of the velocity of the water
through the units. Within each area loading rate, the water velocity
increases as the unit is lengthened.
TABLE 6.14. COMPARISON OF LOADING RATE PARAMETERS FOR SYNTHETIC SEWAGE
System Size
Loading Rates
Area	Weir	HRT
2	3
Area (m ) Length (m)	cm/d	m /m-d	min
9.3
12.2
5.1
0.62
52


10.2
1.24
73


20.3
2.48
40


30.5
3.16
32


40.6
4.95
	
18.6
24.4
5.1
1.24
	


10.2
2.48
172


20.3
4.95
114
27.9
36.6
5.1
1.86
	


6.9
2.51
367


10.2
3.72
105


20.3
7.43
109


40.6
14.86
91
1	3
Selected HRT values for 9.3 m system; data from unit #3 in Bracehouse.
^System operated on intermittent loading condition.
120

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The retention time parameter varies inversely with the area and weir para-
meters. As increasing amounts of wastewater are applied to a unit, the
residence time of that water in the unit decreases. This relation is not
exact because other factors in addition to influent flow rate come into
play. The depth of wastewater in the units increases with flow since the
water is forced to flow around or even over the root mats. This increases
the volume of the system and thus increases retention time above that which
would be predicted by flow alone. Also biomass in the root zone tends to
increase over time due to root growth, sloughed plant parts, precipitate
accumulation and microbial growth. This additional matter can clog pathways
through the root mat, forcing wastewater to find new routes. As this
process proceeds, the root zone may become sufficiently clogged to force
flow above the root mat. Conversely, as the flow rate is increased, the
water velocity may reach a critical value at which particulate matter is
dislodged in the flow and removed from the unit.
In testing some conditions the systems were operated on an intermittently
loaded schedule. During these conditions the retention time was less than
when systems were operated under continuous loading since wastewater was
added up to four times as rapidly as in the continuous operation. Figures
6.29 and 6.30 show hydrographs for the synthetic sewage pilot scale units
operated at 10.2 and 20.3 cm/d loadings, respectively. These determinations
were made by the bucket and stopwatch method. A large block of sewage (the
influent to the first unit shown as curve A) enters the system and passes
through the first and subsequent units rapidly. The influent sewage dis-
places sewage in the system as its momentum moves through; the sewage that
runs off the first, second and third units (shown as curves B, C and D,
respectively) may have entered the system at an earlier application period.
Both loading schedules showed rapid movement of the water in the system.
From the tracer studies it appears that the system holds a considerable
amount of water since peaks immediately following the application peak did
not contain large quantities of the tracer. Rather, the bulk of water with
the added tracer displaced other water in the system and the introduced
water did not leave the system until it was displaced by subsequent
additions.
From water depth measurements in the units it was clear that the volume
contained within the units varied considerably depending on root condi-
tions. Table 6.15 shows volumes in the various units determined from mean
depth. Unit #1 in Hanover was sloped at 0.5% during the 6.9 cm/d condition,
which resulted in a volume that was considerably less than that of the
Cornell unit, which was level. The plants used in each unit were changed
between the 6.9 and 10.2 cm/d conditions. As shown in the table, units 1
and 2 contained phragmites and cucumbers, respectively, in the 6.9 cm/d
condition, while they contained cattails and bulrush in unit 1 and phrag-
mites in unit 2 for subsequent conditions. For some loading conditions of
10.2 cm/d or greater, an intermittent loading regime was used. The inter-
mittent loadings may have produced shorter retention times than if the
system had been loaded continuously.
121

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influent, first unit
Effluent, first unit
Effluent, second unit
Effluent, third unit
6
5
•H
3
1
0
Time (minutes)
Figure 6.29. llydrograph for 36.6 m liracchousc pilot system on lj-27-Ol
(.10.? cm per day, loaded during first Y - 5 minutes of each
haJf hour). These are direct flow measurements over time
for synthetic sewage entering and leaving the system, and
at two intermediate points.

-------
10
9
8
A Influent
B affluent from
first unit
C affluent from
second unit
D Effluent from
third unit
3
1
C
25
10
15
"I i
b'j
«-+
;minutes;
Figure b.30. Direct measurement of flow entering and leaving various
units in Bracehouse system on May 27, 1981. --ows at
this condition, where synthetic sewage was applied for
alternating 30-minute periods, were pulsed much more
distinctly than those where the inf_uent flow was
smaller and more frequently applied (Figure 6.28).
123

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TABLE 6.15. TYPICAL HYDRAULIC CHARACTERISTICS OF A 36.6 m LONG (27.9 m2)
PILOT SCALE NFT UNIT AS ESTIMATED FROM WATER DEPTH MEASURE-
MENTS DURING OPERATION OF THE UNIT
Instantaneous
Area	Average Flow During
Site Loading	Unit Volume, liters	Flow Influent
Rate	tymin Application
cm/d	12	3 Total	Cycle
Cornell
6.9
242
(P)*
37
(C)
251
(R)
530
1.33
1.33
(Synthetic










sewage)
10.2
186
(B)
84
(P)
233
(R)
503
1.97
7.88

20.3
344
(B)
233
(P)
344
(R)
921
3.93
15.72

40.6
456
(B)
419
(P)
363
(R)
1238
7.87
15.72
Hanover
6.9
167
(P)
37
(c)
149
(R)
353
1.33
1.33
(Domestic










sewage)
10.2
158
(B)
102
(P)
188
(R)
548
1.97
7.88

20.3
251
(B)
140
(P)
307
(R)
698
3.93
15.72
*Plant species in each unit shown in parentheses. (B=Cattail/Bulrush;
P=Phragmites; C=Cucumber; R=Reed Canary Grass)
Several generalizations can be made in regard to the volumes contained in
the various units. In almost all cases the volume increased with increasing
flow rate. Within each species tested the volume of water contained in the
root zone increased with increased loading. The exception to this is
phragmites, which contained a larger volume in the first unit than it did in
the second. (As noted earlier, the first units were level or only slightly
sloped, unlike the second or third units, which were sloped at 2%.) As the
flow rate was increased, the surface level rose until flow through the
system was equalized.
With regard to phragmites units, it appears that the slope of the units had
a large effect on the volumes contained. At Cornell, phragmites in unit 2
contained less than half that contained when it was in unit 1, despite the
larger flow at the 10.2 cm/d condition. Similarly, at Hanover the volume
was reduced 40% by placing phragmites in the second position, again despite
the larger flow.
The extent of the interwoven root mat affected the volume of the system in
two ways. Plant species differ in root mass and form with some plants, such
as canary grass, having many small roots while others have large rhizomes
with fewer small roots. The root mat of some plants increased considerably
over the course of experimentation, and this contributed to the amount of
12k

-------
trapped water. The canary grass units usually contained a large volume of
solution relative to other species, even despite its 2% slope. The mat of
fine roots maintained a uniform depth across the units in contrast to plants
with fewer larger roots or rhizomes which channeled or distributed solution
unevenly. The largest root system grown during the course of pilot scale
experiments was the phragmites stand in unit 2, particularly the stand at
Cornell. The root system of napier grass was more extensive than that of
phragmites, but napier grass was not used in pilot scale systems. This
large root mat is reflected in the large volumes this unit held.
Hydraulic retention times for the large pilot scale units are shown in Table
6.16 for the different conditions tested. Some of the same factors which
affect volume also appear to affect retention time. For example, canary
grass usually had a large HRT relative to other units, particularly in the
Hanover unit. The HRT was generally reduced at higher loading rates, as
expected, and was also less when the application period was shortest
(10.2 cm/d condition). Data of particular interest in Table 6.16 are the
sewage HRTs at the 6.9 cm/d condition. Over a short period, HRT increased
by 61 minutes; since plant growth in mid-March was not rapid, it was
hypothesized that the increased HRT was due to trapping of solids.
Cucumbers had only a small root mat which did not cover the unit's surface;
any increase in the HRT of this unit was caused by sedimentation or
attachment of particles or microbial growth and a resulting increase in
volume of the unit.
In the Cornell pilot system, several observations can be made. In general,
HRT did not change a great deal from the 10.2 cm/d loading to the 40.6 cm/d
loading. This is due in part to the higher volumes contained in the units
at successively higher flows and the increasing maturity of the units with
increasing loadings. The phragmites unit in the 40.6 cm/d loading appears
inconsistent with this observation.
D. PLANT CONSIDERATIONS
D.l. Plant Selection Criteria
This section describes the criteria on which plant species were selected for
study and the methods and places of culturing and collection. A wide
variety of plants was tested, often under deficient nutrient and light
conditions similar to those that would be experienced in a treatment
system. Although many interacting variables affected the plants, some
recommendations on the suitability of species for NFT use were possible.
While horticulturalists have used the NFT for plant culture since 1975
(Cooper, 1975), adapting this system to treating sewage requires some
modification. Compared to hydroponic culturing solutions, sewage is low in
nutrients and dissolved oxygen, and contains solid matter which tends to
accumulate in the root zone. In addition to surviving these adverse
conditions, plants must improve the water quality by taking up nutrients and
contaminants and provide the root zone surface upon which microorganisms may
grow. The overall list of the most favorable characteristics in plants is
125

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TABLE 6.16. COMPARISON OF RETENTION TIMES (IN MINUTES) IN 27.9 m2 PILOT SCALE UNITS OPERATING AT
DIFFERENT CONDITIONS WITH REAL AND SYNTHETIC WASTEWATER
Area
Loading
Rate
cm/d
Date

Synthetic Sewage Units
Unit

Date


Real Sewage
Unit
Units


1

2
3
Total

1

2
3
Total
5.1






8/ 6/81
71
(B)
83 (P)
84 (R)
238
6.9
3/ 3/81
121
(P)*
49 (C)
197 (R)
367
3/10/81
66
(P)
17 (C)
70 (R)
153
6.9






3/19/81
86
(P)
24 (C)
104 (R)
214
10.2
5/ 7/81
48
(B)
27 (P)
30 (R)
105
4/22/81
24
(B)
32 (P)
51 (R)
107
13.6






3/26/81
87
(P)
25 (C)
81 (R)
193
20.3
5/28/81
38
(B)
23 (P)
48 (R)
109






40.6
7/ 2/81
44
(B)
9 (P)
38 (R)
91






~Plant Species in Each Unit are shown in Parentheses (B=Cattail/Bulrush; P=Phragraites; C=Cucurabers;
R=Reed Canary Grass).

-------
shown in Table 6.17 and are described below. While possibly no species
exhibit all these characteristics, many species show promise in several
categories.
TABLE 6.17. FAVORABLE PLANT CHARACTERISTICS FOR TREATMENT
OF WASTEWATER IN NFT SYSTEMS
A.	Tolerance to adverse conditions, such as
Standing or flowing water
Anoxic conditions
Sludge buildup and clogging in root zone
Short photoperiods
Temperature extremes and fluctuations
Nutrient stress
Some degree of arthropod damage or disease
B.	Good performance as a treatment medium and a useful crop
High nutrient uptake
High productivity
Extensive root, rhizome and/or shoot system
Production of a valuable commodity
C.	Cultural considerations
Wide geographic availability
Easy and rapid propagation
Minimal maintenance
Not a pest species
Treatment of wastewater by using the nutrient film technique requires plants
that can tolerate excessive water and low oxygen concentrations particularly
for removal of gross pollutants such as BOD and SS. The plants in the
system are intended to treat wastes by their uptake capabilities and by
their root structure. A species that requires extreme aeration and frequent
drying of the root zone is therefore of limited use. In nature, many plants
(such as reed canary grass) are able to withstand the wet spring season
without adverse effects and continual growth in the drier summer months.
The usefulness of these plants depends on their ability to tolerate water
throughout their life cycles. Other species (such as cattails and bul-
rushes) are able to tolerate continuous flooding and anaerobic root zones.
Typical sewage contains many constituents whose decomposition consumes
oxygen. Under conditions of stagnant or slowly flowing sewage, chemical and
biological processes are especially effective in depleting the dissolved
127

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oxygen supply. Since the treatment of wastewater with the NFT depends, in
part, on these processes occurring in the root zone, plant species must be
able to survive low dissolved oxygen concentrations. Most commercially
grown plant species do not tolerate such conditions, and many designs of
hydroponic systems include air spargers or other mechanisms for maintaining
the D.O. near saturation. In commercial NFT systems reaeration is accom-
plished through the turbulence of the thin film. However, depletion of the
oxygen due to decomposition with dissolved organic carbon is faster than the
natural reaeration. While a reaeration step could be added to an NFT unit,
it would be difficult and costly. Some plant species—those of interest for
use in NFT systems—survive and grow quite well in anaerobic sediments
possibly due to an ability to translocate oxygen downward through the plant
tissue to the roots for respiration (Hutchinson, 1975).
The majority of the nutrients and carbonaceous material in sewage is in
colloidal and particulate form rather than dissolved. The physical means to
treat these solids in an NFT include sedimentation, filtration and adsorp-
tion, all of which result in material deposition in the root zone. Although
this may provide material for anchoring the plant's roots, it is a highly
carbonaceous mass that characteristically has no dissolved oxygen present
and contains reduced materials. This mass may inhibit gas exchange to the
roots and create an anaerobic sludge layer into which plant roots cannot
penetrate. Plants with fine root hairs may suffer clogging from fine
particles sooner than would plants with large roots or rhizomes.
Since the NFT is viewed as a year-round treatment system, plants are
required that can grow, or at least remain viable, through the short
daylight periods of winter. Ideally the plant would not go dormant, but
would continue to grow year-round, taking up nutrients and extending roots.
At a minimum the roots should provide a living surface to support physical,
chemical and microbial processes. Photoperiod and light intensity, like
temperature, are variables that can be operator controlled. The addition of
lights can extend daylength or increase the light intensity, while black
covers can simulate darkness. A number of plants, for example, chrysanthe-
mum and poinsettia, are stimulated to flower under short day conditions and
will not grow vegetatively. If flower formation is desirable during long
day conditions, an artifically short day can be provided with a black
cover. New cuttings need a long day to form a stem and leaves. This longer
day can be artificially provided with artificial light. On the contrary, at
higher latitudes the light intensity in winter is often not sufficient for
adequate photosynthesis. To achieve good plant growth artificial lighting
with high intensity lights is necessary. This is energy- and capital-
intensive .
Temperature fluctuations in the form of excessive heat in summer and the
lack thereof in winter may be stressful to plants. While the hypothesized
NFT system includes a greenhouse covering for the plants, this provides both
a help and a hinderance: it allows operation of the system through the
winter but requires cooling in the summer. A cost-effective system mast
minimize the energy input for temperature maintenance. Also, since green-
houses with limited temperature control are subject to greater variations
128

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and fluctuations, the plants should be tolerant of considerable daily
temperature fluctuation.
In contrast to a horticultural hydroponics system, a waste treatment NFT
uses a fairly dilute nutrient solution. While the N-P-K ratio of a typical
hydroponics solution is 200-40-100, the ratio of a medium strength sewage is
40-15-12. In addition, the nutrients in sewage may be in organic compounds
that must be microbially degraded before nutrients are freed for plant
uptake. The available nutrients then may be even lower than the low N-P-K
ratio. While this low level of nutrients can support plant life, some
nutrients such as potassium may be limiting in a sewage solution. Nitrogen
in sewage occurs in the organic or ammonium form. The organic nitrogen is
decomposed to ammonium. In recent years it has been shown for several crops
that NO 3 -N is the preferable nitrogen form and that the ammonium ion can
cause toxic effects when present in higher concentrations. This seems to be
important in NFT systems (Langhans, personal communication; Massey and
Winsor, 1980). Also, some micronutrients, such as sulfur, boron and iron,
may be deficient and limit the growth of tomatoes and other plant species
(Wallace et al., 1978).
Due to the adverse conditions for NFT plant production, less vigorous plants
may be more subject to attack by parasites or disease than would vigorously
growing plants. Because some levels of disease and pest infestations are to
be expected in the NFT systems, effective methods of control requiring
minimal labor should be developed.
Because one of the goals of NFT treatment is nutrient removal, high nutrient
uptake by the plants is important. Plants with the capability of fast root
growth and nutrient scavenging would not only remove nutrients from solution
but would also be well suited in other ways such as providing increasing
surface area for bacterial growth.
The production of a valuable plant end product would decrease the system's
operating costs and demonstrate an additional value of the NFT treatment
system. The production of flowers, for example, would generate an income to
the system above and beyond the special handling costs involved. All plants
grown in an NFT system would have some value as forages or biomass, but
crops such as ornamental or medicinal plants would yield a much more valu-
able end product. The production of non-edible plants would be desirable
because problems with toxic materials would be avoided.
Another desirable characteristic of NFT plants is that they would have a
wide geographic availability. This would allow for more standardization of
system designs and easier collection of material. A wide geographical
distribution may also indicate that a plant is able to grow under a variety
of temperature and light regimes, which is desirable for year-round NFT
operat ion.
In addition to having wide tolerances, plants in the NFT that can be propa-
gated quickly and easily would be highly desirable. If a species chosen has
a short yet productive lifespan, it will need frequent replacement. For
example, annuals such as Japanese millet grow to maturity in less than three
129

-------
months, at which time they require harvest and replanting. In contrast,
perennial grasses which root quickly from cuttings can be propagated in a
wastewater stream. A complicating factor in this comparison, however, is
that species with lower economic value tend to be the easiest ones to
propagate.
The last criterion is that plants selected should not be a pest species.
Many plants have become pests by their introduction to new areas. Examples
of these plants in the U.S. are kudzu, water milfoil and water hyacinth. A
plant with the capability of rampant and damaging growth should not be
introduced in an NFT system, regardless of the performance in the system
(unless special precautions were taken to ensure the plant was contained in
the system). Consultations with professional botanists should precede the
use of non-native plant species in the system.
Since many different types of plants can be grown in NFT systems, there is a
hierarchy of end uses of the plants. These uses can be ranked on the basis
of end product value and also according to the number of possible end
products. For example, many plants can be used as biomass, but a smaller
number is available for food production. When one considers the number of
species available for use in the NFT and their economic value, an inverse
relationship between these two variables becomes evident (Figure 6.31).
Using plants for biomass production is the least valuable disposition of
plant material. Essentially all plants grown could be composted or
processed for energy recovery. Flower or ornamental crops could produce a
significant income to the system, and concern for contaminant uptake would
be minimal.
Many food and forage crops can be grown using NFT. During this project reed
canary grass and other forage species have been grown successfully, and an
elevated protein content has resulted in reed canary grass when grown in
solution culture. Similarly many vegetable species have been grown success-
fully hydroponically. While food production may yield a larger income to
the system than would biomass production, more stringent quality control
requirements may be imposed on the system. For example, vegetables would
need to be free of pathogens and heavy metals, which would necessitate
extensive testing and possibly some pretreatment. In many cases the
production of vegetables would not be an option.
Crops with very high value such as pharmaceutical herbs could also be grown
in NFT, but the problems of quality control that would have to be solved
would be significant.
For any crop the overriding criterion for its selection must be its ability
to perform the wastewater treatment required. If a group of plants meets
this requirement then plants can be selected based on other desirable
characteristics or end products.
130

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o
•H
CO
-P
O
Flower or
c
ber
oo
3i
Kunber of Species (increasing-h
_gure 6.31. Relation between the number of species
included in an end product, and the relative
value of that end crcduct.
131

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D.2. Species Tested
A number of emergent aquatic plants were selected for initial study. Plants
that had performed well in commercial hydroponic systems were added after
hardy plants were identified. These plants included both food and
ornamental crops. Other plants were added to the test program on the basis
of some known desirable characteristic. For example, napier grass grows
very fast and propagates easily. The list of plants used in this study is
shown in Table 6.18.
TABLE 6.18. PLANTS GROWN IN NFT UNITS OR EXAMINED FOR CULTURAL
PROPERTIES DURING THE STUDY
Aquatic or wetland plants
Arrow arum (Paltandra virginica)
Big burreed (Sparganium eurycarpum)
Bristly sedge (Carex cotnosa)
Soft stem bulrush (Scirpus validus)
Burreed (Sparganium americanum)
Common cattail (Typha latifolia)
Japanese knotweed
(Polygonum cuspidatum)
Lake sedge (Carex lacustris)
Lizard tail (Saururus cernuus)
Phragmites (Phragmites australis)
Soft rush (Juncus effusus)
Swamp loosestrife
(Decodon verticillatus)
Woolgrass (Scripus cyperinus)
Dryland plants
Reed canary grass (Phalaris arundinacea)
Eastern cottonwood (Populus deltoides)
Napier grass (Pennisetum purpureum)
Vegetable or food plants
Blackberry (Rubus spp.)
Comfrey (Symphytam spp.)
Cucumber (Cucumis satiuus)
Japanese millet
(Echinochloa frumentacea)
Sugar cane (Saccharum officinarum)
Tomato (Solanumlycopersicum)
Wheat (Triticun aestiuum)
Wild rice (Zizania aquatica)
Flowers
Chrysanthemum
Carnat ion
Fushia
Geraniums
Lantana
Marigold
Petunia
Rose
Strawflower
(Helichrysum monstrosum)
132

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D.3. Culturing Considerations
D.3.a. Obtaining Plant Material—
Following the selection of plant species, those available in nearby wetlands
were collected. The majority of species were collected in late summer and
fall of 1980. Reed canary grass was collected many times over the course of
the project since it was a major test species. Similarly, large quantities
of phragmites were collected in November 1980, and cattail and bulrush in
March 1981 to plant in the pilot scale units. A typical stand of phragmites
is shown in Figure 6.32.
The location and date of collection of the species that were collected are
shown in Table 6.19. Plants were removed from their habitats using garden
tools, transported in plastic bags and placed in NFT units. Any soil or
dead growth that was collected with the plant material was removed at the
greenhouse before the material was placed in the units. All species were
placed in one or two of the three meter units in the Ken Post greenhouse,
and large quantities of certain species collected were placed in the larger
6.1 meter units.
TABLE 6.19. COLLECTION DATA FROM WETLAND PLANTS GROWN IN THE NFT
Propagation
Species	Type of Propagule	Location	Date
Arrow arum
Big burreed
Bristly sedge
Burreed
Common cattail
Japanese knotweed
Lake sedge
Lizardtail
Phragmites
Reed canary grass
Soft rush
Soft-stem bulrush
Swamp loosestrife
Woodgrass
entire plant
rhizome with shoot
sod
rhizome with shoot
rhizome with shoot
rooting branch tips
sod
rhizome with shoot
rhizome
sod
entire plant
rhizome with shoot
rooting branch tips
sod
Montezuma Wildlife Refuge	9- 7-80
Montezuma Wildlife Refuge	9- 7-80
Cornell Agronomy ponds	8-28-80
Cornell Agronomy ponds	8-28-80
Town of Dryden, NY many
Cornell University	6-15-81
Town of Dryden, NY	8-29-80
Montezuma Wildlife Refuge	9- 7-80
Montezuma Wildlife Refuge	9- 7-80
Cornell Agronomy ponds many
Cornell Agronomy ponds	8-28-80
Cornell Agronomy ponds	8-28-80
Montezuma Wildlife Refuge	9- 7-80
Cornell Agronomy ponds	8-28-80
The most actively growing or most viable plant parts were collected. Where
plants reproduced vegetatively, those reproductive parts were collected.
For species in which little vegetative spread occurred, the entire plants
were harvested.
133

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Stand of phragnites at Montezuma Wildlife
Refuge
134

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In addition to those plants harvested intentionally, seeds, roots or viable
parts of other plants were inadvertantly collected. All of these species
grew to some extent with the primary species in the NFT unit and were
monitored. A list of these volunteer plants is presented in Table 6.20.
TABLE 6.20. VOLUNTEER SPECIES WHICH SURVIVED TRANSPLANTING
WITH OTHER WETLAND SPECIES
Bedstraw (Galium sp.)
Bittercress (Cardamine pennsylvanica)
Buttercup (Ranunculus sceleratus)
Duckweed (Lemna minor)
False nettle (Boehmeria cylindrica)
Jointed rush (Juncus articulatus)
Purple loosestrife (Lythrum salicaria)
Water parsnip (Sium suave)
Several commercially grown plant species were propagated from seeds, stem
cuttings and root cuttings. These plants, along with their sources and
transplanting form, are given in Table 6.21. In general, seeds were not
germinated directly in the NFT because they would either be washed away or
fail to develop a supporting root system. Figure 6.33 shows one attempt to
root canary grass in stream by using capillary matting. Seeds of reed
canary grass and Japanese millet were germinated on "bubble plastic." Most
other seeds were germinated in "Jiffy 7" peat pots in a misting house.
These are small pots which consist of dried compressed peatmoss. They were
expanded with water, and seeds were planted in premade holes in the tops.
Periodic misting (approximately 10 seconds every 10 minutes) kept seeds from
drying out without depriving them of oxygen. Flowers from Horticulture and
Ornamental Floriculture were started as cuttings which were rooted in peat
pots on a mist table. Other flowers and vegetables were obtained in plant
flats or pots containing some potting soil. Before these plants were placed
in the NFT units, the .soil was removed from the roots. Attempts to
germinate seeds in peat pots placed directly in NFT units were unsuccessful;
apparently roots could not grow into the water-saturated peat. Some plant
cuttings devloped rapidly in the nutrient film. Napier grass and sugarcane
are two such species whose cuttings (stem sections with one or more nodes)"
grew to new plants directly in the nutrient solution (see Figure 6.34).
Subsequent cuttings from the resulting napier grass stand were successfully
propagated in both nutrient solution and sewage.
135

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Figure 6.33. White canary grass roots forming a root
mat below capillary matting used in
seeding trials.
-l-jUlWNII
Figure 6.3*+. Napier grass, sugar cane, and cottonwood
cuttings (left to right.) after placement
in the 1IFT 3 meter units. The bubble pack
plastic was used to partially control algae.
Cottonwood cuttings are in "Jiffy T" peat
pots.
136

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TABLE 6.21.
SOURCES AND PROPAGULES
OF COMMERCIAL PLANTS GROWN IN NFT UNITS
Form
Plant
Source
Seed
Seed
Cucumbers
Reed canary grass
Bruinsma Seed Co., N. Ridgeville, OH
Brulnsma Seed Co., Mecklenburg, NT
Seed
Seed
Wild rice
Millet
Kester's Wild Game Food Nurseries, Inc.
Omro, WI
Seed
Seed
Strawflowers
Chrysanthemums
Geo. W. Park Seed Co., Inc.
Greenwood, SC
Cuttings
Cuttings
Napier grass
Sugarcane
Univ. of Puerto Rico, Rio Piedras, PR
Cuttings
Cottonwood
Boyce Thompson Inst, for Plant Research
Rooted
cuttings
Blackberries
Agway, Inc., Ithaca, NY
Plants
Plants
Plants
Geraniums
Fuchsia
Lantana
J. Bartlett's, S. Sudberry, MA
Plants
Plants
Plants
Geraniums
Cucumbers
Tomatoes
George's, Newark Valley, NY
George's, NH
Plants
Plants
Plants
Plants
Chrysanthemums
Carnations
Roses
Petunias
Cornell Dept. of Horticulture and
Ornamental Floriculture
Plants
Marigolds
Kings Dept. Store, W. Lebanon, NH
Tubers
Comf rey
backyard plant - E. Falmouth, MA
D.3.b. Growing Plant Material—
Carbon-free nutrient solutions were used to grow plants in Ken Post green-
houses at Cornell, whereas sewage was used for propagation in the Hanover,
New Hampshire, greenhouse.
The nutrient solution used in Ren Post was composed of the following
ingredients:
137

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Peter's Hydrosol soluble fertilizer
Calcium Nitrate
Chelated iron
The hydrosol fertilizer had a low N:P ratio compared to sewage, so addi-
tional nitrogen was added as Ca(N03)2« The iron was added as a supplement
to the fertilizers since many plants, particularly reed canary grass, had
shown Fe deficiencies earlier in the study. The strength of the solution
was maintained by monitoring the conductivity and subsequent nutrient addi-
tions. To prevent non-nutritive salt buildup, the entire solution was
periodically replaced.
D.4 Plant Care During Crisis
2
The large scale of these NFT experiments where 9 to 28 m of plants were
used provided an opportunity to study the pest interactions with the
plants. In all cases, control programs were kept to a minimum. Only when
the pests appear to threaten large sections of the unit were solutions
applied.
Pest control alternatives were always identified with the assistance of
expert analysis. Pest control measures applied at infrequent times during
the study included resting of the system to enable root drying and regenera-
tion, chemical application, and biological controls.
E. ANALYTICAL APPROACH
E.l. Computerized Summary of Information
All data from pilot scale experiments was entered into disk storage on a
Terak computer. Data were stratified by experimental phase, experimental
site, the variable tested, the sampling point in the unit where the data was
taken, and the sampling date. Data from Cornell pilot units were divided
into three groups: (1) data from Phase 0 pilot units, (2) data from 12.2
and 24.4 meter units in Phase 1, and (3) data from the 36.6 meter nailti-
species units in Phase 1. Hanover data was also divided into three groups:
(1) data from the CRREL greenhouse in Phase 0, (2) data from the CRREL
greenhouse in Phase 1, and (3) data from the Hanover greenhouse in Phase 1.
Bench scale unit data from both Cornell and Hanover units was also entered,
each experiment's data grouped separately, but able to be recalled by date.
Data from the loading rate simulation (varied flow) experiments at both
Cornell and Hanover were evaluated manually. Separate files contained
long-term data, such as temperature and incident radiation, which was
collected over the duration of the experimental period.
E.2. Long Term Analysis
To facilitate understanding of long term trends, plots of each variable were
produced showing the chronological trend over the entire experimental
138

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period. These plots included all variables that were routinely determined
in the pilot scale units, as well as environmental conditions in the green-
houses .
The arithmetic average for each condition was determined using the following
method. The first two measurements were deleted at each condition, and any
remaining data points that varied more than twice the standard deviation
were deleted.
E.3. Sensitivity of System to Controlling Parameters
Treatment means of each variable were calculated for the pilot scale experi-
ments. These means showed the performance of the variables in each unit or
in each part of the units tested under each of the loading rates used. In
addition, regression and multiple regression analyses were used to determine
correlations between variables or variables and parameters.
E.4. Formulation of Predictive Relationships
Presently there are few methods of predicting the results of exposure of
wastewater to a plant system. In an attempt to separate the different
mechanisms of control, the above computerized data were examined for
empirical relationships between the following variables: grow rate, loading
rate, area and weir loadings, temperatures, hydraulic retention time (where
available), depth of liquid, mass of plants and/or roots, plant yield, and
entrapped microbial mass. The goal of this effort was to be able to predict
explicit quality when given certain easily measured or anticipated operating
conditions for an NFT unit.
139

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CHAPTER VII
RESULTS
The growth of plants in the nutrient film process as adapted for wastewater
treatment was begun in early summer of 1979, and some of the same test units
continue in operation some three years later during the preparation of this
report. The bulk of the research activity took place in a 24-month period
(October 1979 through September 1981)—with a 6-month initial overview
referred to as Phase 0, and an 18-month intensive investigation of the para-
meters that control the process referred to as Phase 1. It should be noted
in reviewing the data that the experimental program was designed to develop
the feasibility of the nutrient film technique as a total treatment system
for gross pollutant removal as well as nutrient control. The sequence of
events emphasized treating domestic sewage to a reasonable effluent quality,
and subsequent NFT sections were to use this effluent as a nutrient source.
Each test condition was considered to be complete when a relatively constant
effluent quality was obtained under a given set of conditions. This experi-
mental approach led to rapid development of information on the potential of
the system but resulted in conservative estimates of the potential capacity
of the system. Under most of the test conditions studied, the plant species
were exposed to heavy loadings of sewage after one to six months of growth
under non-optimum conditions. This exposure of young plants to pollutants
resulted in test units that accumulated root masses from 1 to 5 cm in
depth. In contrast, under field conditions after several years of applica-
tion it would be anticipated that the mature nutrient film would accumulate
root systems of 20 to 30 cm deep with dense stands of the plant materials.
The primary test species that was chosen was reed canary grass since it is
found throughout most of the United States and is common in wastewater land
application treatment systems. It was hoped that this species would grow
and survive under heavily loaded conditions in a cold climate with minimum
attention for pest control and other special conditions. The results indi-
cate that this was the case and that reed canary grass is a potential candi-
date for several different applications of wastewater treatment, although it
is probably not the optimum species for many applications. An interesting
laboratory result illustrates the kind of observations that were experienced
in this study. Laboratory technicians conducting the total Kjeldahl nitro-
gen analysis of the crops were worried about the accuracy of the tests when
it was observed that the reed canary grass continuously produced nitrogen
contents throughout the year of 5 to 6% of the total dry weight of the
grass. The total crude protein content approaching 40% is representative of
a valuable animal food and is not known to be the quality of forage produced
by field stands of reed canary grass. The fact that the nutrient film
application to relatively dilute nutrient solutions was able to do this
illustrates one potentially useful by-product of this system.
ll+0

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The following data presentation Is an attempt to select typical results from
a large amount of data collected from a greater than a 15-person-year effort
by a large number of Individuals. The detailed data appendices provide com-
prehensive Information on domestic and synthetic sewage pilot scale studies.
A. CHRONOLOGICAL DEVELOPMENT OF TREATMENT CAPACITY OF NFT SYSTEMS
The data were developed using continuously fed units that varied In size
from 12.2 to 36.6 meters long (40 to 120 ft), and application rates varied
from 0.47 to 11.36 m /day (125 to 3000 gal/day). The use of these rela-
tively large systems at high application rates Is referred to as pilot
applications; since they were conducted In low-cost greenhouses (one of
which was built at the Hanover, New Hampshire, treatment plant) the data are
a good approximation of what could be obtained at full scale facilities.
Examples of the chronological data development illustrating the variability
in both the influent and the effluent parameters are discussed in the fol-
lowing paragraphs. Plots of all pilot scale data for all conditions are
shown in Appendices B and C for domestic and synthetic wasterwater,
respectively.
The treatment efficiency (using synthetic wastewater) by the Cornell NFT
units is illustrated In Figures 7.1 and 7.2. Influent COD was targeted to
simulate a medium strength sewage at around a 200 mg/& concentration,
excepting for the initial runs, which were conducted at around 350 mg/£.
The total COD of the effluent remained low throughout most of the testing,
even though data from the unit (shown in Figure 1) indicate that the unit
was operating under winter light conditions in the Northeast and at an
Increasing loading rate. Total effluent COD concentrations less than 50
mg/& were typical, excepting for the higher loading conditions (Condition 4
at 40.6 cm/d). Increasing loading rates and the relationship to average
radiation are shown in Figure 7.2 of this greenhouse situation.
Similar chronological data development for the application of domestic sew-
age to the nutrient film is shown in Figure 7.3. The data indicate that
although the influent COD ranged from around 250 mg/£ up to 350 mg/A the
total COD of the effluent was around 100 mg/£. In the mature systems tested
at the lower loading rates the effluent COD was often less than 30 mg/A. An
example of the comprehensive data collected with the domestic sewage treat-
ment unit is shown in Figure 7.4, in which six major variables are illus-
trated for the spring of 1981 using the primary settled domestic sewage pro-
duced by the Town of Hanover, New Hampshire.
The general trend in wastewater applications illustrates the attempt to
achieve high loading rates during the experimental period. Also throughout
the period temperature fluctuations occurred in these systems since minimal
controls were built Into the plastic greenhouses.
The suspended solids removal efficiency is one of the more promising aspects
of this system. As can be seen at the top of Figure 7.4, the effluent
solids were often less than 10 mg/£ and showed only a small effect of
Increased loading rates. BOD values of less than 20 mg/A were achieved at
the lower loading rates but increased to around 50 mg/& at the higher
141

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• Oond. !+
Cor id i t, i.on 3
Condit ion
I 00
1 6

6

u.
;)
Feb.	March	April	May	June
Figure \ . inl'luent. and eiTluent. chemical oxygen demand i'or the Cornell
NFT treat i. (if!; synthet j c wastewater in spring, lc)0l. Condit ion:;
correspond to ureal loadings of 6.9, 10.2, ?0.3, and 'l0.6
centimeters per day, res pee t, i ve I y for a 36 in lony unit.

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lino
300
200
100
• Influent
© Uf fluent
• _ •



14 0
30
20
10
20
• •
• ••
• • ••
efts*80 ® & <$(*&%
® ®
'®
©

®
®
® 
-------
)|00
Condition 2
300
uo
6
200
Q
O
CJ
100
19
May
29i
2
9
19
April
10
9
March
Figure 7.3. rnfMuont and effluent chemical oxygen demand for
the Hanover NKT treating domestic wastewater in
spring, 1981. Condi Lions-, correspond to area!
loadings of 6.9, -10.2 and 20.3 cm per day,
respectively, l'or a 3('> in long unit.

-------
Ln
o cc
-^c-.
30
2")
10
Greenhouse Air
Maximum o
Minimum •


O C
O o
3 0°0 CC«M0o 0°
o
• • •
>u uou °
3 CD O n °
-------






Inf
luent
•










Effluent
0




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•
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13 20	30 1 5 13 23 lb	1c	2o 1
March	April ••'lay	June
19°1
risr:re ".4c. Example	of chronological data development for domestic sewage
testing with the :IFT at Hanover, "lev Hampshire.
lUo

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loading rates. Subsequent data presentation will examine the relationship
between loading and hydraulic retention time and effluent quality. It Is
Important to note that the Influent sewage temperature In these systems dur-
ing the winter often was 7° to 8°C, thus causing problems In achieving
efficient pollutant removal.
The large number of uncontrolled variables caused analysis of the results of
this study to be a difficult problem. The process efficiencies must be a
function of temperature, light period and radiation Intensity. However, the
effect of light period and radiation intensity did not appear to be a major
variable for BOD and suspended solids removal when the plants were in a
healthy condition. This most likely is related to the fact that the removal
of these constituents is a biological/physical/chemical phenomenon not
related to plant uptake. Of course, it was anticipated that nutrient uptake
would probably be a function of the plant productivity and microbial acti-
vity in the root zone. Figure 7.5 is a data summary of the productivity of
reed canary grass grown for long periods of time under limited light and
nutrient conditions.
B. CHARACTERISTICS OF THE NFT IN TREATING SYNTHETIC WASTEWATER AND DOMESTIC
SEWAGE
Long-term operation of pilot scale NFT models with synthetic wastewater and
actual domestic sewage enabled the parametric evaluation of the process.
Tables 6.12 and 6.13 present a summary of the steady state conditions for
these continuously fed pilot units operating on synthetic and domestic
wastewater, respectively. Between November of 1979 and August of 1981 a
total of 16 steady state conditions were observed with synthetic wastewater
and 13 conditions were examined with domestic sewage in Hanover. Twenty-two
pollutant variables were examined in the synthetic wastewater, and seventeen
variables were tested in the domestic sewage units. Each steady state con-
dition is an average of a number of analyses, between four to fifty-five,
depending on the analysis. Results with each of the major variables will be
discussed briefly In the following section. Examination of the relation-
ships of loading rates and mechanisms responsible for the following observa-
tions will be discussed in subsequent sections.
The variables that are compared in this study are listed in Table 7.1.
B.l. pH
The wastewater in both the synthetic and domestic studies varied only
several tenths of a pH unit and remained slightly on the alkaline side of
neutral. Synthetic wastewater was usually Introduced around a pH of 7.0 and
through the first unit varied approximately 0.2 to 0.3 of a pH unit in the
first unit. A variation of approximately one pH unit occurred in the 36.6
meter unit with an effluent of 7.7 to 8.0. The domestic sewage unit varied
less, with an Influent pH o? around 7.3 and an effluent slightly lower than
the influent values, with a value of 6.9 to 7.0 quite common. The limited
variation in both synthetic and sewage units suggests that microalgae growth
was not a significant factor affecting pH in the mature steady conditions.
147

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1 11 2.1 31
Octobe r
1979
10 20 30 10 ?0 30
November December
9 19 29
January
19^0
8 18 28
February
9 19 29
March
8 10 20 8
April	May
''if/.ure 7 .'ja. Incident ra.d in I. iori and yield oi' reed canary |.<;ra::;s grown in continuing Nl'T
culture in the Cornell liraeehouse over' the course of exper i. merit at. Ion. The
un.it was used as a synthetic sewage test unit from October 1979 to November
198(1, after which it was used as a biomass and evapotranspirat.ion control
unit for larger systems.

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7 17 27 7 IT ?7 6 16 26
^ 1U 2U U 1^4 2k
r
15 ^5
i:
M:i.y	Juno	July	August	September October November	December
.1960
Kigure '(. "?b. [ncident radiation and yield of reed canary ^rass grown iri continuing
culture in the Corne.l 1 Hmeehouae over the eourue of experimentation. 'l'he
unit was uued asi a tjynfchctie sewa;;;e teat unit from October 1979 to November
1980, after which it wan used as a biomasa and evapotranspiration control
unit for 'harder systems.

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lit 2h
1?
February
J ariuu ry
March
April
J une
Figure
Incident, radiation and yield of reed canary ^rana ^rown in continuing
Nl'T culture in the Cornt* Li Bracehouse over the course of experimentation.
The unit was used as a synthetic sewage teat unit from October 3 979 to
November 1980, after which it wtiii used as a biomar.::. and evapotraruspi ra-
tion control unit for larmier systems.

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Table 7.1. VARIABLES MEASURED IN TESTING PILOT SCALE NFT SYSTEMS WITH
SYNTHETIC AND DOMESTIC SEWAGE.
Synthetic Sewage Variables
Flow
pH
Dissolved Oxygen
Air Temperature
Chemical Oxygen Demand
Soluble Chemical Oxygen Demand
Ammonia Nitrogen
Soluble Ammonia Nitrogen
Nitrate Nitrogen
Soluble Nitrate Nitrogen
Organic Nitrogen
Soluble Organic Nitrogen
Total Phosphorus
Soluble Phosphorus
Total Solids
Total Volatile Solids
Total Suspended Solids
Volatile Suspended Solids
Zinc
Cadmium
Solar Radiation
Relative Humidity
Domestic Sewage Variables
Flow
pH
Dissolved Oxygen
Air Temperature
Chemical Oxygen Demand
Soluble Chemical Oxygen Demand
Ammonia Nitrogen
Nitrate Nitrogen
Total Nitrogen
Total Phosphorus
Soluble Phosphorus
Suspended Solids
Volatile Suspended Solids
Solar Radiation
Biochemical Oxygen Demand
Total Organic Carbon
Sewage Temperature
The microalgae was a problem in the early stages of operation and testing
(before plants developed a dense canopy), but it was not a variable in the
steady state values.
B.2. Dissolved Oxygen
The dissolved oxygen around the roots in an NFT system is a critical para-
meter for plant production and pollutant removal. Typical dissolved oxygen
profiles in the unit are presented In Figures 7.6 and 7.7. Figure 7.6 com-
pares the effect of loading rate and continuous application to the inter-
mittent applications during much of the synthetic sewage study. This infor-
mation indicates that intermittent application provides some control over
the dissolved oxygen in the wastewater. Figure 7.7 compares the dissolved
oxygen in the continuously operated synthetic wastewater unit with domestic
sewage applied intermittently. Although intermittent application enabled
the dissolved oxygen concentrations to be maintained at an average value of
2 to 3 mg/£ at loading rates of 10 cm/day, it is clear that dissolved oxygen
at high loading could be a problem. Low dissolved oxygen at high loading
rates is likely. These values will be useful in interpreting the subsequent
lack of nitrification that is presented in the nitrogen removal information.
151

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.tinuou
cntmuous

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Synthetic, continuous
S ewage , i n L e rrn i I, t. e n t,
15 on/15 off
Sewage, intermittent,
30 on/30 off
Loading Rate
em/d rn 3 / m—ci
6.9
10.? 3.71*
Dat t?
2/13/81-3/l^/Bl
'1/13/81-5/ 8/81
5/ 7/81-6/ 3/8L
12 1>I 16 18 20 22 2h 26
Sample Locat j on , rn Downstream
K Lguro 7.7.
Die;l'.o I veil oxygen concentration in multispecies NI'T unit treating primary
oeltLcd domestic :.;ewage at Hanover, New Hampshire.

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B.3. Temperature
No attempt was made to control the wastewater temperature In any system, so
the influent temperatures In the wintertime varied with the domestic sewage-
studies. The low temperature in January averaged 7.6°C with a standard
deviation of 2.4°. The effluent during this month was 12.8°C, with a stan-
dard deviation of 0.5°. At other times of the year, common values of sewage
in New Hampshire was 9 or 10°C. This temperature would increase by an
average of 3° or 4°C going through a 12-meter long unit. Increases in sew-
age temperature during the summertime ranged from 14°C to as high as 24.8°C,
with a standard deviation of 6°C. A potential increase in temperature as
sewage moves through the system needs to be taken into account in evaluating
the applications of the technology.
While few synthetic sewage temperature measurements were made, some genera-
lities can be made. In the Cornell Bracehouse air temperature is quite sen-
sitive to solar radiation; and since a large volume of synthetic sewage was
prepared in the greenhouse, the Influent temperature of this sewage
approached the average air temperature in the greenhouse. This Influent
temperature ranged from about 12°C in winter to about 17°C In summer. Simi-
lar small rises in temperature were measured in synthetic sewage as it
passed through the units, although only a small number of these measurements
were made.
B.4. Suspended Solids
The suspended solids data generated from the synthetic wastewater system are
of limited value since the influent feed had few solids. The suspended
solids reported in Table C.21 in the appendix for synthetic wastewater
represents microbial growth in the feed at the room temperature. This
varied from 2 to 8 mg/£. However, the concentration of effluent suspended
solids from the unit is a function of the large accumulation of microbial
solids in the root system that are continuously lost as the microbes grow
and slough from the root mass. At a loading rate of 10 cm/day, the typical
effluent solids from the synthetic wastewater was approximately 7 mg/£.
Suspended solids concentrations in the effluent from the synthetic studies
seldom exceeded 20 mg/£.
The effluent suspended solids with the sewage studies represent some of the
more important information from this study. Influent total suspended solids
concentration varied widely from a dilute 35 mg/£ in the wintertime to a
high of 140 mg/A. Effluent solids were usually less than 30 mg/£ and in the
36-meter long unit varied between 4 and 16 mg/£ up to a loading rate of 20
cm/day which was the highest loading tested. A typical effluent solids pro-
file through the NFT unit with reed canary grass and in a multiple species
unit is shown in Figure 7.8. An analysis of the influence of various para-
meters on effluent solids concentration will be given in a subsequent sec-
tion.
An important observation related to suspended solids was the nature of the
suspended solids within the system. It had been envisioned that much of the
solids would become entrapped in the root mass and that this material would
154

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DISTANCE FP.GM INFLUENT, n
Figure 7-3- Mean suspended solids concentrations in
KFT systems treating domestic wastewater
at 10.2 cm/d. Curve A shows a 12 m canary
grass system with a relatively high SS
influent. Curve 5-C-D shows a three-part
N7T system consisting of phragr.ites ,
cucumbers, and canary grass (ir. order from
influent).
155

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remain In the unit. Observations of the Hanover domestic sewage Indicated
that the suspended solids were rapidly flocculated Into relatively large
clumps of material. These materials settled rapidly but also moved around
within the system on an Intermittent basis. This observation leads to the
possibility of flushing the system with rapid additions of the wastewater.
B.5. Turbidity
As Indicated In the suspended solids removal section, the NFT produced a
relatively clear effluent. Turbidity measurements with the NFT system
treating domestic sewage indicated a reduction in a 12-meter length from
around 50 JTU to less than 20 in almost all cases. At a loading rate of
20 cm/day during June on reed canary grass a typical decrease from 29 to
6 JTU was recorded.
B.6. Chemical Oxygen Demand
A comparison of changes in total and soluble COD for the synthetic waste-
water is shown in Figure 7.9. In most cases the COD was rapidly removed,
and at 5 cm/day decreased from 250 mg/i to 60 mg/Z in a 12-meter section.
Since the influent organics were mainly soluble, the total COD closely fol-
lowed the soluble COD in the synthetic wastewater. Figure 7.10 compares the
influence of temperature and maturity on similar systems at the higher load-
ing rate of 10 cm/day. As the system became more mature, it was clear that
loadings of 20 cm with a 12-meter system were effective in removing COD down
to low levels. The influence of the length of the system on soluble COD is
shown in Figure 7.11 where two single species units of different lengths
were compared with a multiple species units of a longer length. These data
clearly indicate that the process is highly efficient in removing organics
and that there appears to be a relationship between loading rates and the
length of the unit. This will be examined in subsequent sections.
A comparison of the total COD removal profile to soluble COD with domestic
sewage is shown in Figure 7.12. Because of the influence of suspended
solids there is a larger difference between these two parameters than was
present in synthetic sewage. Total COD removal appears to be similar to
synthetic wastewater. A larger fraction of soluble COD appears to be
nonbiodegradable in domestic sewage. This is indicated by lower percentage
reductions of soluble COD in domestic sewage; also all COD in synthetic sew-
age originated as sucrose.
Organic removals are examined in Figure 7.13 by comparing the total BOD to
the soluble BOD in domestic sewage. At a loading rate of around 7 cm/day
the effluent from the second section approached 30 mg/I of BOD, and the
effluent from the third section averaged 10 mg/il in most runs. Doubling
that application rate increased the effluent concentration from the second
unit to 50 mg/i BOD and 33 mg/£ from the third unit. At a 20 cm/day loading
rate this increased to an average of 41 mg/I from the effluent from the
third unit.
A comparison of the winter operation to the summer operation with respect to
the total COD removal efficiencies is shown in Figure 7.14. In addition to
156

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30C
SOLUBLE
20C
CK
ICO
12
m system
m system
TOTAL
x
200
100
2C
10
30
0
AREA LOADING RATi, cm/d
Figure T-9* Reductions of total and soluble
chemical oxygen demand concentrations
in synthetic wastewater through 12 m
and 36 m systems. Note similarity
between total ar.d soluble COD for
both influent (solid symbols) and
effluent (open symbols) synthetic
wastewater.

-------
100
¦>.n
CO
¦fc'?.
w
I H
H
•'<
§
s
U
>-•
0
w
! <
C_>
1	I
(ii
Ci.
M
, 1
:5
o
w
Average Air Temperature
Hirfi
1/ ?/80
3/1Y/80
6/17/80
O 12/ 6/79
4 2/21/80

-------
3CQ
9.3 iTi2 Canary Grass
13.6 m2 Canary Grass
2T.9 e2 Multi-species

a
o
:>->
X
<
o
M
o
100

from iriri/j:
DI3TA
Figure 7.11. Influence of system length on soluble
CCD removal from synthetic sewage at
an area loading rate of 20.3 cm/d.
Systems A and B contain reed canary
grass only, while System C consists
of cattails and bulrushes, pnragnites,
and canary grass (in order from influent}.

-------
33C
SOLUBLE
200
100
Influent
Ef fluent
'OT.
200
—13
ICO
20
10
o
AREA _,CADING RATt, cir./d
Figure 7-12. Reductions of total and soluble
chemical oxygen demand concentra-
tions in domestic sewage in 36 m
multiple species N7T units, which
were tested at three different
loading rates.

-------
100
SOLUBLE
40
20
TOTAL
3
s
I 120
<
10.2 cm/d
100
i—
s
Q
I
20.3 cm/d
UO
20
n
12
DISTANCE FROM INFLUENTS, m
Figure 7.13. Total and soluble oxygen demand concentra-
tions at various biochemical points in 36 m
multiple species unit treating domestic
vastsvater. Three loading rates are shown.
lol

-------
Uoc
A. warm Temperatures
a ?4n
20	3C
DAY 35" OPERATIC1!-.
to
w
Q
a
>¦
x.
o
400
320
240
loO
80
s:
3
la Temperatures
12	2b
DAY OF OPERATION
"igure T . iL . Comparison of COD removal from
domestic savage during relatively
varm (A) and cola {2) temperatures
O	= Influent
^	= Effluent from first unit
¦+	= Effluent from second unit
~	= Effluent from third unit

-------
the effect of temperature this figure shows a significant influence on BOD
removal with length. This is most likely related to the hydraulic retention
time in the channel.
B.7. Nitrogen
In general, the nutrient removal for continuous flowing units was low. This
was primarily related to the fact that most of the tests conducted were at
high loadings to achieve high BOD and suspended solids removal rates.
Therefore, the nutrient loadings on these plant systems at high organic
loading rates should not be expected to result in efficient nutrient manage-
ment. Example reduction of total nitrogen with the synthetic wastewater
shows effects of the length of the units in Figure 7.15. Maximum reduction
of nitrogen in these units occurred at the lower loading rates of 5 cm/day
and achieved a maximum of 12 to 15 mg/£. of nitrogen removed from the sys-
tem. Figure 7.16 compares total Kjeldahl nitrogen removal at varying load-
ing rates. The relationship of plant yield and other nitrogen removal
processes will be discussed later.
Since the influent synthetic sewage contained urea nitrogen, hydrolysis of
urea to ammonia occurs rapidly in the synthetic wastewater. The ammonia
concentration in the sewage represented a major change in nitrogen species
as shown in Figure 7.17. Approximately 10 mg/fc of ammonia nitrogen was
removed in these systems at the lower loadings, and at high loading rates
the removals were less.
The intent of testing the nitrogen cycle was to define conditions under
which nitrification could be achieved so that a denitrification system could
be added as one means of removing nitrogen from the system. However,
throughout the study there was nearly a complete absence of nitrification.
Although numerous analyses were done for nitrate nitrogen for both synthetic
wastewater and the domestic sewage, the highest concentration of nitrate
observed in the effluent was 4 mg/Jl in one early spring operation at a low
loading rate of 5 cm/day. At higher loading rates almost no nitrates were
observed. Although this was a surprising observation, it is most likely
related to low hydraulic retention times that were observed under most test-
ing conditions and the higher organic loading rates that were used to define
the kinetics of the organic solids removal. All of the loading rates tested
resulted in low dissolved oxygen values that could inhibit nitrification.
Additional analysis of this lack of nitrification in the systems will be
reviewed under the root analysis section.
B.8. Phosphorus
The phosphorus removals were similar to the nitrogen removals in that the
total efficiencies were relatively low. A comparison of the total phospho-
rus removed from the synthetic wastewater at the 5 and 10 cm/day loading
rates is shown in Figure 7.18. Maximum removal efficiency occurred in the
early summer with the 5 cm/day loading rate, resulting in about a 50% remov-
al of total phosphorus. A similar comparison is given in Figure 7.19 for
total phosphorus concentration changes in the NFT units treating domestic
sewage. Total reductions of 2 or 3 mg/i. (amounting to about 30 percent
163

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A 9-3 m2
3 18.6 m2
C 27-9 m2
Canary Grass
Canary Grass
Multi-species
	I	I	
12	24
DISTANCE FROM INFLUENT, m
Figure T.15-
Total nitrogen concentration of syntheti
wastewater in three different NFT system
at an area loading rate of 10 cm/ti.

-------
2C -
Influent.
13	~
iffluent
C	10	20
ARIA LOADING RATI, crn/d
Figure 7-lS- Total K.'eldahl nitrogen concentration
reductions in 9-3 m2 reed canary
grass units using synthetic wastewater.

-------
30
20
^ Influent
_ Intermediate
Points
10
O Effluent
C
12	2k
DISTANCE FROM INFLUENT, m
Figure T.17. Ammonia nitrogen concentrations of
synthetic sewage at various sampling
points in NFT systems. Curves A and
5 show 9.3 m2 systems at 1C.2 and
5.1 cm/d loadings, respectively, while
Curves C and D show 19.5 and 27 • 9 ni2
systems, respectively, both at loadings
of 10.2 c~/d.
lot

-------
• 5-1 cm/d loading
O10.2 cm/d leading
	I	I	
0	12	2k	36
DISTANCE FRCM INFLUENT, r.
Figure 7-l3. Total phosphorus concentrations of
synthetic wastewater ir. different
length systems. Curves A and B show
the effect cf increasing loading.
Curves C and D show effect of increasing
length on canary grass [C) ar.d multi-
species (D) systems.
lb

-------
cm/d leading
O 10.2 cm/d loading
& 20.3 cm/d loading
36
0
12
DISTANCE FROM OFLUEET, n
"igure T.19. Total phosphorus concentrations of
domestic sewage at different loading
rates to two systems. Curves A and 3
show a smaller removal at a higher
loading for 12 m systems , and Curves C
and D show the same for 36 ™ systems.

-------
removal) of the phosphorus were achieved at many of the loading rates. At
increasing loadings of 10 to 20 cm/day the change in phosphorus concentra-
tions decreased to only a little more than 1 mg/£.. Figure 7.20 compares the
ortho-phospate and total phosphorus concentrations of influent and effluent
sewage. There is a consistent difference of a little less than 1 mg/£
between total and ortho-phosphorus.
B.9 Indicator Organisms
Fecal coliform analysis was conducted on several samples for the domestic
sewage studies. These data show that approximately 90% of the fecal coli-
form was reduced in one 12-meter length unit. Additional information needs
to be developed in this area.
B.10.	Zinc and Cadmium
Changes in concentration of zinc in the synthetic wastewater were studied
briefly, whereas the removal efficiency of cadmium was studied throughout
the testing period. Figure 7.21 summarizes the few observations on zinc
removal that were made and indicates an efficient removal at loadings of 5
and 10 cm/day of wastewater. The influence of the loading rate and the
length of the unit on the cadmium concentration is shown in Figure 7.22.
The influent concentration varied from 0.1 to a little more than 0.3 mg/£
and was rapidly reduced within the first few meters of length in the nutri-
ent film to less than 0.06 mg/H in most cases. It is likely that the effi-
cient removal of cadmium is a function both of the anaerobic solids in the
root zone and the plant uptake of cadmium in the initial section. The up-
take of heavy metals in the influent part of the nutrient film system would
have to be examined carefully in terms of ultimate disposal or use of the
plant products.
C.	PROCESS SENSITIVITY AND POLLUTANT REMOVAL MECHANISM DEFINITION
The data presented in the previous section indicates that the NFT is capable
of producing secondary quality or better effluent from primary settled sew-
age and a soluble synthetic wastewater. This information, however, does not
indicate the reasons or the mechanisms responsible for controlling the pol-
lutants or the limitations in the process. This section is intended to sum-
marize the data further and present information on small-scale experiments
designed to clarify several pollutant removal mechanisms.
C.l. Hydraulic Retention Time Effects
Perhaps the most critical parameter in controlling pollutant removal in any
reaction scheme is the length of time that the pollutant is exposed to the
removal mechanism. In the NFT system the hydraulic retention time indicates
the duration that the pollutant can be acted upon both by the root system as
well as by the entrapped microbial mass. Increased hydraulic retention in
the unit can also be an indication of increased depth of liquids, and this
will in turn affect the diffusions of oxygen pollutants to the bacteria and
the roots. The ability to predict the hydraulic retention time in any given
system in which the substrate removal rate is known would allow a unit to be
169

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INFLUENT TC NFT SYSTEM
'V
EFFLUENT FROM r.FT SYSTEM
TIME, days
Figure 7-20.
Influent ar.d effluent concentrations
of phosphorus in domestic sewage in
the Hanover NFT system, which began
operation on February lo , 1981.
G = total phosphorus
¦+ = crtho phosphorus

-------
.0
Influent
z'
. o
+ Effluent
. 2
.3
. 4
o.c
6o
3o
100
40
2C
0
TIME, days
Figure J.21. Influent and effluent zinc concen-
trations of synthetic sewage in a
reed canary grass pilot scale unit
First day of analysis was July 25,
1980.

-------
• 5.1 cm/d loading
q 10.2 cm/d leading
^ 20.3 crr./d loading
DISTAi-I
FROM EFFLUENT, m
figure 7-22.
Cadmium concentrations of synthetic
wastevater in different length systems
under several leading conditions.
Curves A and B represent 9-3 m2 units,
and Curves C and D represent lS.6 m2
units. Curve E represents a 27-0
unit.
rr.

-------
designed based on the required hydraulic retention time. Originally, It was
thought that increased microbial mass and root mass should increase the
hydraulic retention time. This increase in retention time would be a
reflection of the advantage of the nutrient film system over other biolog-
ical contacting systems.
The information shown in Figure 7.23 relates the hydraulic retention time to
the hydraulic loading rate in a 36-meter long unit and was obtained from
tracer testing with NaCl. Hydraulic retention times at loading rates of 10
cm per day in these units were 100 minutes. Although this should be suffi-
cient time for an organic carbon interaction and suspended solids removal,
it is a limited time span for nutrient interactions. This short hydraulic
retention time suggests that for specific pollutant removal, such as sus-
pended solids, operation of the unit in a manner that would increase the
hydraulic retention time would achieve better efficiency of pollutant remo-
val than was achieved by the semi-continuous operation tested. For example,
a decrease in the slope of the unit would result in increased hydraulic
retention time. Another method of changing the hydraulic retention time
would be to use an intermittently loaded and unloaded unit as if operating a
multiple batch operation. Several of these alternatives were tested in
short-term experiments with sewage at the Cayuga Heights, New York, treat-
ment facility and are discussed elsewhere.
The effect of maturation of the NFT system on the hydraulic retention time
in the system is shown in Figure 7.24. This indicates that the hydraulic
retention time may double as the root mass and the suspended solids in-
crease. Although this is valid, it has a loading rate limitation, as will
be shown In short-term studies. Application of high loading rates on mature
systems actually results in a shorter retention time, most likely because of
a short-circuiting that occurs after the suspended solids are entrapped in
complex root mass. Additional work will be needed in the area of hydraulic
retention time to define the influence of root mass and solids accumulation
on this Important parameter.
C.2. Loading Rate Effects
There are four loading rate parameters that need to be considered in defin-
ing this process. First is the hydraulic, or area, loading rate, which is
the equivalent depth of wastewater applied to the surface area of the system
over a day's time (measured as centimeters per day). Second are mass load-
ing rates of nutrients or pollutants in terms of mass of constituent added
to the reactors per unit area of the system (measured as kilograms per
square meter per day). Third is the weir loading rate, or the volume of
wastewater added to the system divided by the width or cross-sectional area
(measured as liters per meter of width per day). Fourth is the overflow
rate which is similar to upflow velocities that occur in a clarifier. (The
weir loading in terms of cubic meter per meter width of the unit results in
the average liquid velocity that would occur in a clarifier where the sur-
face area is the area of the NFT unit).
If empirical relationships could be developed between treatment performance
and loading rates, it would provide a highly simplified method of sizing NFT
173

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Uoc
s 300
200
C
3 ICO


• Total HRT

i
>





X Intermediate Sections




R = canary grass




C = cucumber




? = phragmites

R


B = bulrush/cattail

C





(

1



R
R (
>
P

D —




r -
"p ¦



3
D.
3



1

10
a-J
40
AREA LOADING RATE, cm/a
Figure 7.23- Hydraulic retention times of a 36 ra
ITFT system using synthetic wastewater
17-

-------
o
a
e
o
i—i
j
<
cc
c
>-
250
:0C
150
100
50


• To.al KRT

1
1


X Intermediate


Sections
(
~
^ R = canary grass


C = cucumber


P = phragir.it es

R




-

P

P

3-10-31
3-19-31
Figure J.2k. Hydraulic retention ^ime of the
3o m system using domestic waste-
water loaded at 10.2 cm/a.
lv5

-------
systems. The hydraulic retention time masks the effects of many parameters
such as pollutant concentration; however, if there is a loading rate rela-
tionship between the hydraulic addition and the effluent quality, it
provides the simplest method of sizing the system. It is more likely that
the mass loading rates will be easier to manipulate in empirical relations
since the pollutant removal mechanismswill be more closely related to the
amount of the pollutant added per system area per unit time. Other
variables such as suspended solids, may be more easily explained by weir
loading rates than mass loading rates since they are removed by the physical
process of sedimentation. The weir loading rate is directly related to the
hydraulic velocities in the system. Typical results achieved with the
steady-state conditions with the synthetic and domestic sewage systems are
presented in the following sections.
C.3. Loading Rate Relationships with Synthetic Wastewater
The ranges of pollutant mass loading rates, steady state mass removal rates
and removal efficiencies for pilot scale system tests with synthetic sewage
are summarized in Table 7.2. Hydraulic loading rates varied from 5.1 to
40.6 cm/d over the testing period, with most rates around 10 cm/d (see Table
6.12). Detailed tables showing specific loading rate data are presented
later (Table 7.22).
TABLE 7.2. RANGE OF POLLUTANT LOADING RATES TESTED WITH SYNTHETIC SEWAGE
IN PILOT NFT UNITS. TARGET HYDRAULIC LOADING RATES VARIED
FROM 5.1 to 40.6 cm/d.
Parameter
Range of Measured
Range of Observed
Range of Pollutant

Mass Loading Rate
Steady State
Removal Efficiency

kg/ha-d
Removal Rate
% of Added


kg/ha-d

COD
133-972
46-414
32-91
Soluble COD
101-903
87-461
51-88
N
13-141
5- 20
6-57
P
6- 50
0- 4
1-52
Cd
0.020-0.509
0-0.285
0-91
Chemical oxygen demand removal efficiency showed a small effect of loading
rate, but COD treatment was also affected by other factors as well. Figure
7.25 shows the relation between treatment efficiency and mass loading rate,
and it indicates that a wide range of efficiencies occurred at some rela-
tively low loading rates. Hydraulic retention time appeared important in
characterizing COD removal, and HRT relationships will be presented later.
In addition, temperature and radiation appeared important in COD removal.
Removal rates compared to^loading rates are shown in Figure 7.26, which also
shows the small effect (r ¦ .36) of loading rate.
Since the synthetic sewage was composed of soluble rather than particulate
or colloidal material, little difference was expected between total and sol-
uble COD. This was generally the case, as Figure 7.27, which describes mass
176

-------
100
Go
p
w 60
M
O
M
E*h
h,
w
Uo
" 1 c>
ft
PC
20
~
o
A
A
A
200
© ~
A
® 9.3 m2 systems
0 18.6 m2 systems
£ 27.9 in2 systems
O
> o
J-
X
uoo	600
'L'OTAl, COD MASS I.0ADTN0 KATR, kg/ha-d
800
1000
l-'itfure '(.25. Relationship between total COD removai efficiency and total COD mat-
rate for conditions Un-ited with synthetic sewage.
loading

-------
18.6 m2 system:
«
o
o
27.9
*+00
0
6oo
800
1000
TOTAL COD MASS LOADING RATE, kg/ha-d
Figure '(.26. Relationship between total COD may;.; loading rate and total COD mass removal
in the NJ'T' systems treating domestic sewage.

-------
Hoc;
c)
Synthetic Sewage
10.6 m2 system;
600
Uoo
800
1000
0
SOLUBLE COD MASS LOADING RATE, kg/ha-d
figure T-?T. Relationship between soluble COD mass loading rate and soluble COD mass removal
in the Nl'T systems treating synthetic sewage.

-------
removals of soluble COD, closely parallels removals shown in Figure 7.26.
Total COD was slightly higher than soluble COD in the effluent, indicating
that some solids generation had occurred in the systems.
Total nitrogen showed an inverse relationship with nitrogen loading (r =
.24). Figure 7.28 shows removal efficiency of N as a function of N loading
for three different size systems. Soluble nitrogen showed the same trend,
with a higher correlation (r^ 3 .56) as shown in Figure 7.29. Further
analysis of N will be presented in Section 7.E.
Phosphorus efficiency was scattered in general, but followed a rough inverse
relationship also. Figure 7.30 shows total phosphorus removal as a function
of P loading rate (r2 = .30). Similarly, Figure 7.31 shows this relation-
ship for soluble P (r2 = .16). Other factors such as oxidation reduction
potential are important for P control and will be discussed later.
C.4. Primary Settled Domestic Sewage Loading Rates Effects
The range of mass loading rates, mass removal rates, and process efficien-
cies examined with sewage in the 4 to 20 cm/day loaded units are summarized
in Table 7.3. Maximum treatment efficiencies for BOD and suspended solids
exceeded 90% whereas the maximum removal of nutrients was always less than
30%. The temperature, time of year, irradiation and sewage concentration
values are significant factors in the efficiency of the process. Figure
7.32 shows the relationship between BOD treatment efficiency and loading
rate for all of the steady conditions examined in this study. This figure
indicates that there appears to be a good relationship between removal
efficiencies and loading rate. The observed BOD removal rates in relation
to the loading rate are shown in Figure 7.33. As the figure indicates, the
NFT process is quite efficient at loading rates of up to 200 kilograms per
hectare per day. Such a relationship does not follow as closely for the TOC
although there are limited data as shown in Figures 7.34 and 7.35.
Similar loading rate relationships for total and volatile suspended solids
are shown in Figures 7.36 and 7.37. High treatment efficiencies of
suspended solids were achieved at loading rates exceeding 200 kilograms per
hectare per day.
The loading rate-removal efficiency relationships of nitrogen and phosphorus
are more scattered (see Figures 7.28, 7.38 and 7.39). Although there
appears to be a relationship, it will be necessary to consider plant uptake
and other mechanisms before NFT nutrient removal can be defined.
All of the data for sewage temperature were examined to determine whether it
was a significant variable with the sewage system. The operation of the
units in the winter climate in western New Hampshire gave us an opportunity
to examine the temperature and light limitations on the NFT process. The
following figures show the effect of influent sewage temperature on BOD and
total organic carbon (Fig. 7.40), suspended solids (Fig. 7.41), and nitrogen
and phosphorus (Fig. 7.42). These data indicate that there is no influence
of temperature on these pollutant removal mechanisms, possibly because only
a small range of temperatures was experienced. Although the influent
180

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~













O
~
9.3
18.6
m2
m2
systems
systems


A



A
27-9
rn2
systems
—
0








-
O A
O
A

O





—


A





1

1
1
1

1

1

1
0	20	ho	60	Bo	TOO	120	lhO
TO'J'AL N MASS LOADING RATE, kg/ha-d
Figure
Relationship beLween total nitrogen removal efficiency
and total nitrogen mass loading rate for conditions
tested with synthetic sewage.

-------









A







~


©
9.3
in2
systems




~
18.6
m2
systems




a
27.9
m^
systems

0







A







G
O






&






-

A









1

1
A

• »
1
1

0	20	1|(>	60	80	100	120	lUO
SOI .IJHT.E N MASS LOADING RA'l'E, kg/ha-d
figure 7-?9- Relationship between soluble nitrogen removal
efficiency and soluble nitrogen mass loading
rate for conditions tested with synthetic sewage

-------




0
9.3
m2
3 y S "0-23LS
—
0


~
18.6
n2
systsns
-



A
27.9
m2
SjSZ 31S
-


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—
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o
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1


~
0	1C	20	SO-
TOTAL P MASS LAODING RATZ, kg/ha-d
Figure 7-30. Relationship between total phcsp.iorus
removal efficiency and total phosphorus
mass loading rate for conditions tested
with synthetic sewage.
lo3

-------

O 9-3 m2 systems

O
30
_ ~ 18.6 m2 systems

A 27-9 m2 systems
^ kr,
—


o

2

bl



o

£ 30
O
w

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<
~
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o

1 20

10
AO

0 A 0 o

A 0

1 A 1
o	10	20	30
SOLUBLE ? MASS LOADING RATE, kg/ha-d
Figure 7.31. Relationship between soluble phosphorus
removal efficiency and soluble phosphorus
mass loading rate-for conditions tested
with synthetic sewage.
13^

-------
ICO
80
~
~ Q
60
O
~
uo
20
100
200
;00
BOD LOADING RATE, kg/ha-d
Figure J.32.
Relationship tetveen BOD removal efficiency
ar.d BCD mass loading rate for conditions
tested with domestic sevase.
155

-------
200


1^1
100	200
BOD LOADING RATE, kg/ha-
3C0
Figure 7.33. Relationship between BOD mass loading
rate and E0D mass removal in the NFT
systems treating domestic sewage.
186

-------
130
o
w
M
o
b-
fc:
2
E-
jADII'IG RATE, kg/ha-d
Figure 7.3^.
Relationship between TOG re.-noval efficiency
ar.c. TOC xass loading rate for conditions
tested v^i-h domestic sewage.
157

-------
r}
l
t3
LOO
75
<
o 50
co
25
igurs
¦ 35 •
TOC LC
100
)ING RATI, kg/ha-d
Relationship between TCC mass loading rate
and TOC mass removal in the NFT systems
^rea'cing domestic sewage.
133

-------
200

^ 150
1—1
M
o
Q
2
.0
IOC
SUSPENDED SOLIDS LOADING RATE, kg/ha-d
Figure Too. Relationship between suspended solids mass
loading rate ar.d suspended solids mass
removal in the N7T systems treating domestic
sewage.
1S9

-------
¦C 2C0
i
c>
<
>
o
s
J
I—I
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>
230
13C
150
0
VOLATILE SUSPENDED SOLIDS LOADING RATE, kg/ha-d
Figure 7.37. Relationship between volatile suspended
solids ir.&ss leading rate and volatile
suspended solids mass removal in the
I'FT systems treating domestic sevage.
190

-------
I6T
o
2
0 - c
_i
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o
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	I	I	I	I	I	I	
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PHOSPHORUS LOADING RATH, kg/ha-d
Figure Y• 39• Reiat.i on:;h L p between phosphorus mass loading rate and phosphorus mass
removal 1'or conditions tested with domestic: sewage.

-------
100
>H
o
s
a
CO
60
S3 i
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2C
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o
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O TOC
_l	
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:t?L'Jznt
o
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iMPZRATUKI, °C
16
"igure 7.40. Effect of temperature on the treatment
efficiency of E0D and TOO in N7T systems
using domestic sewage.
193

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IOC
INFLUENT T3-TERATURE,
igure 7-^1.
Effect of ^enroerature on the treatment
efficiency of total and volatile suspended
solids in NFT systems using domestic
sewage.
19k

-------
B
influent
12	1U
TEMPERATURE,
Figure 7.^2.
Effect of temperature cn the treatment
efficier.cy cf nitrogen and phosphorus
in N7T systems using domestic sewage.
195

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TABLE 7.3. RANGE OF POLLUTANT LOADING RATES TESTED WITH DOMESTIC SETTLED
SEWAGE IN PILOT NFT UNITS. TARGET HYDRAULIC LOADING RATES
VARIED FROM 5.1 to 20.3 cm/d IN PILOT UNITS, WITH INTERMEDIATE
LOADING RATES OF GREATER THAN 200 cm/d. ONLY THE TOTAL UNIT
RESULTS ARE SHOWN HERE.
Parameter
Range of Measured
Range of Observed
Range of Pollutant

Mass Loading Rate
Steady State
Removal Efficiency

kg/ha-d
Removal Rate
% of Added


kg/ha-d

BOD
58-272
44-166
61-91
TOC
42-132
26- 97
51-73
COD*
133-556
99-247
44-74
SS
20-206
17-164
59-94
N
13- 68
2.8-12.7
6-30
P
2.97-13.0
0.38-1.77
5-32
~Limited amount of data
temperature ranged to less than 7°C, the collection of solar energy in the
plastic greenhouse raised the sewage temperature to 14 to 15°C for a
significant portion of the day in all cases. Thus the temperature
differentials between summer operation and winter operation were usually
less than 10°C and often only 5° or 6°C. This effect was apparent on a cold
day. When the outside air temperature was minus 20°C, the average influent
sewage temperature to the greenhouse was 7°C; and the effluent sewage
temperature was often around 22°C.
C.5. Plant Environmental Factors
A number of factors were anticipated to influence plants in such a way that
it would make them of limited value for wastewater treatment in the NFT.
Dissolved oxygen concentrations, temperature, and light intensities all have
the capability of controlling plant health and growth rate. The dissolved
oxygen concentrations in units heavily loaded with BOD were low. Reed
canary grass grew poorly, and patches of it died when area loading rates
were significantly greater than 20 cm per day. It appeared that this load-
ing rate would eventually kill all the reed canary grass. Figures 7.43 and
7.44 show the differences between a healthy stand of reed canary grass and
the condition of reed canary grass after a long period of application at
loading rates of 20 cm per day with synthetic wastewater. Similar observa-
tions were made during domestic sewage applications. In both domestic and
synthetic sweage, when the loading rates were decreased or eliminated, the
grass recovered rapidly. In one series of experiments the reed canary grass
was allowed to turn completely brown. Subsequent additions of tap water
enabled the reed canary grass to recover over a period of several weeks.
After this period of time the grass appeared to be normal. Thus the influ-
ence of overloading has the capability to damage the grass, but it is pos-
sible for it to recover when loading rates are relaxed.
196

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Figure 7-^3. Healthy canary grass in Unit 3, Bracehouse.
»
Figure 7-^i+. Brown canary grass in Unit 3, Bracehouse.
197

-------
Napier grass was found to be particularly sensitive to low dissolved oxygen
levels. In one series of experiments the liquid flow was eliminated and the
napler grass was allowed to stand for about 24 hours in a non-aerated water
with the roots submerged in 20 cm of water. The apparent lack of oxygen to
the root system caused death of the napier grass. Subsequent tests where
the napier grass was Inundated with 15 cm or more of water for shorter peri-
ods did not have this effect.' Plants known to be capable of surviving an
anaerobic root zone such as cattails, bulrushes and sedges were affected
little by high organic loading rates and low D.O. when roots were completely
inundated by water and solids.
Additional information on factors affecting plants is presented in Section
7.F on plant culturlng.
C.5.a. Temperature—
The temperature of the wastewater, as indicated earlier, had a relatively
minor effect within the range tested. However, the combination of season,
light intensity and temperature did have a significant impact on one spe-
cies, phragmltes. Phragmltes has the characteristic of dying back when the
photoperlod is sufficiently shortened. During several months of the winter,
the NFT sections of phragmltes appeared to be dead; however, Inspection of
the roots showed that they were living. As soon as the daylight period
increased past the threshold for growth, the phragmltes rapidly developed
new shoots and started to grow again. The influence of this kind of plant
on a waste treatment system would need to be carefully evaluated. The BOD
and suspended solids removal might continue during the cold period as long
as the roots survived.
C.6. Root Mass Influence
The roots on many of the species tested developed into large masses on the
impermeable surfaces of the NFT units. Root depths of greater than 5 cm
with phragmltes and reed canary grass were common after several months of
growth. More dense and deeper roots were achieved with the napier grass,
where 10 cm or deeper mats were observed. Although solids were entrapped in
the root mass, the effect of root growth on entrapment of solids was not as
significant as had been anticipated. In many cases the flocculated solids
in the sewage system appeared to be "light and fluffy" and often floated to
the top of the roots rather than being entrapped within them. Due to the
destructive nature of sampling to obtain root mass determinations, little
data In this area were developed. However, a non-destructive technique to
measure entrapped solids was developed and used throughout much of the study
with the synthetic wastewater and the domestic sewage studies. Examples of
the accumulation of total and volatile solids are shown in Table 7.4. Typi-
cal entrapped mass solids equalled one thousand grams per square meter of
reactor. It is Interesting to note that at a liquid standing depth of 5 cm
this results in a concentration of entrapped mass of approximately 20 grams
per liter. Even if the liquid depth were 10 cm this would be equivalent to
10 grams per liter of entrapped solids. This high concentration of solids
indicates the potential that the system has to manipulate the pollutants if
it were possible to manage these entrapped solids efficiently.
198

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TABLE 7.4.
TOTAL AND VOLATILE ACCUMULATIONS OF MATERIAL IN THE ROOTS OF
PLANTS TESTED IN 27.9 m PILOT UNITS USING SYNTHETIC AND
DOMESTIC WASTEWATER. VALUES ARE g/m . VOLATILE VALUES ARE
PARENTHESIZED.
Sewage Type Species
Distance From
Influent End
of Unit
m
Area Loading Rate, cm/d
10.2
20.3
40.6
Synthetic
Cattail/
3
1113(156)
921(359)
510(372)

Bulrush
6
	
909(409)
642(411)


11
101(101)
424(305)
448(354)

Phragmltes
3
17(8)
264(203)
670(529)


6

150(78)
404(314)


11
199(76)
404(267)
624(487)

Canary
3
1057(772)
899(611)
2119(1526)

Grass
6
	
568(409)
528(370)


11
956(717)
687(467)
323(174)



Area Loading Rate,
cm/d



6.9
10.2
20.3
Domestic
Cattail/
3
		
745(477)
766(230)

Bulrush
6
824(313)
1044(73)
275(102)


11
	
562(450)
774(294)

Phragmltes
3
	
507(451)
267(174)


6
86(52)
1191(107)
90(50)


11
	
559(492)
211(137)

Canary
3
	
3705(3520)
982(638)

Grass
6
1008(625)
6423(385)
622(354)


11
	
4955(297)
998(599)
Table 7.5 converts the data for entrapped solids to an accumulation rate for
solids for the various applications and various loading rates tested with
the pilot units. There appears to be little accumulation with increased
loading rates as shown by the variability of the data. Typical accumulation
rates of 20 to 30 grains per m /day were observed in the lower loading rates.
C.7. Bench Scale Simulation
An attempt was made to develop a short term test that would simulate the NFT
and enable the kinetics of pollutant removal to be more carefully defined
with various plant species. In effect, an equivalent to the BOD bottle test
was sought to measure the pollutant removal capabilities of the NFT system.
Short term, small unit studies using two meter long by 10 cm wide units were
199

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TABLE 7.5. TOTAL AND VOLATILE ENTRAPPED SOLIDS IN THE ROOT ZONE OF
PLANTS TESTED IN 27.9 m2 PILOT UNITS USING SYNTHETIC AND
DOMESTIC WASTEWATER. VALUES ARE g/m2-d. VOLATILE VALUES
ARE IN PARENTHESES.
Sewage Type
Species
Distance From
Influent End
of Unit
m
Area Loading Rate,
10.2 20.3
cm/d
40.6
Synthetic
Cattail/
3
74(10)
35(14)
31(19)

Bulrush
10.7
7(3)
16(12)
22(18)

Phragmites
3
	
11(8)
34(26)


10.7
12(5)
16(11)
31(24)

Canary
3
53(39)
43(29)
106(76)

Grass
10.7
48(36)
33(22)
16(9)



Area Loading Rate,
cm/d



6.9
10.2
20.3
Domestic
Cattail/
3
	
47(30)
18(5)

Bulrush
6.1
15(6)
104(7)
11(4)


10.7
	
35(28)
18(7)

Phragmites
3
	
32(28)
7(4)


6.1
2(1)
119(11)
4(2)


10.7
	
35(31)
5(3)

Canary
3
———
154(147)
27(17)

Grass
6.1
19(12)
584(35)
22(13)


10.7

206(12)
27(16)
developed (as described in the Material and Methods section [Chapter VI,
Section A.3.6]) under the bench scale laboratory model of tests. Eight
experiments were conducted from January to July, 1981 with synthetic waste
water and five experiments were conducted with the domestic sewage in New
Hampshire. The synthetic wastewater was tested with phragmites, reed canary
grass, bulrush, soft rush, bristly sedge, and several other species; and a
control with no plants was run with all bench scale tests. The control
consisted of an empty unit which was exposed to sunlight. In most of the
experiments the wastewater was recycled continuously, and evapotransplratlon
was compensated for with distilled water. In several instances with both
sewage and the synthetic wastewater the units were tested on a one-pass, or
once-through basis to obtain removal rate information that would be similar
to the one pass systems tested at the pilot scale. These were referred to
as the varied flow experiments where a range of hydraulic rates were tested
simultaneously.
200

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The relationship between the hydraulic application rate and the resulting
hydraulic retention time in the varied flow experiments as measured by
tracer studies are shown in Figure 7.45. Although these reactors were
smaller, the hydraulic retention times were similar to the larger units. A
comparison of the hydraulic volume of each unit to the flow rate is shown in
Figure 7.46 indicating the dependence of the equivalent depth of liquid in
the systems at increasing flow rates. The water quality data from the units
were quite erratic since mature canary grass was taken from the operating
units tested. In these small units the entrapped mass was disturbed, and
suspended solids data were of limited value. The COD removal rates in
the first varied flow test ranged from 15 grams per m /day up to a maximum
of 104 grams per m2/day at an equivalent area loading of 147 cm per day with
a hydraulic retention time of 58 minutes as measured at the beginning of the
experiment. This changed to 28.9 minutes after six days of use. In most
cases the total suspended solids concentration in synthetic wastewater
increased in the effluent thereby indicating production of solids in these
units.
The influent phosphorus concentration varied from 15 to 21 mg/A, and the
resulting uptake was not sufficiently accurate to define removal kinetics
with a one pass small scale system. The pH was 7.5 throughout most of the
runs in the first varied flow experiment. A relationship similar to phos-
phorus was also observed with the influence of nitrogen in the single pass
systems. A comparison of the total COD removals achieved is given in Figure
7.47 comparing the influent COD at between 200 and 360 mgfl to the effluent
achieved over a period of days with reed canary grass at a loading rate of
20 cm/day. This information was developed in the first varied flow experi-
ment in June, 1981, and similar information is comparable in the second
synthetic wastewater experiment conducted in July, 1981.
The average BOD concentrations are shown for synthetic wastewater in Figure
7.48, which compares the control unit to the effluent achieved at loading
rates of 18, 38 and 74 cm per day. The decreasing efficiency of the process
is apparent in this comparison.
C.8. Experiments with recirculating Bench Scale Models
Recirculating batch experiments were conducted with all pollutant parameters
in several species to try to obtain an estimate of the removal kinetics and
the removal efficiencies that could be obtained after extended exposure in
the system. Although a great deal of- data was obtained with these experi-
ments there are many questions that remain in relation to the validity of
the small scale tests. Example information comparing several variables from
these experiments are included in the following section.
Evapotransplratlon data from the bench scale experiments showed the signifi-
cant influence of plant-water uptake over the control. Only the recirculat-
ing water was examined. Figure 7.49 compares a control in the winter where
the greenhouse temperature was approximately 15°C to the summer control and
the evapotransplratlon measured with napier grass in summer. It is surpris-
ing to note a large Increase in evapotransplratlon over the controls, since
literature values indicate that the water evaporation from water surfaces is
201

-------
lUo
IOC
li
3

50
^3
J
<
cc
G
>-
X
60
UO
d.'j
19
AREA LOADING RATE, ern/d
37	7^
1^9
T
I	I
• Experiment Start-up (7-23-Sl)
O Experiment 2nd (U-31-Sl;
1
1
20 40	80	160
HYDRAULIC LOADII'IG P.ATZ, ml/min.
lSc
20C
Figure 7.4?. Hydraulic retention times in the tench scale
varied flow experiment using synthetic waste-
water .
202

-------
AREA LOADING RATE, cm/d
HYDRAULIC LOADING PA.
Figure 7-^6. Hydraulic volume contained in bench sca_e
varied flow units ax different loading rates
of domestic sewage.
20 3

-------
~ _n:^uent
+ affluent
TutE, aavs
Figure
i?otal and soluble
concentrations
: j ¦
vastevater to a reed canary grass
"bench scale ur.it loaded at 20 cr./d.
First day of analysis vas -June 12, 19cl.
20U

-------
2l;0
22';
200
1 Y!>
150
12^
100
75
50
r- 25
w
2 0
x
o
o 200
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@ 17',
CJ
o lr,0
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12t)
100 Ifr
Control
38 ern/d
lb
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J 8 cm/(1
T'l cra/d
_L
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J-
X
X
X
X
X
X
X
X
3 't ¦? 6 7
DAY OK EXPERIMENT
9 10 0
3 'i 5 6 7
DAY OK EXPERIMENT
10
Figure T.'iB.
Effluent biochemical oxygon demand concentrations of different units in the varied
flow experiment, which be^aii July 20, 1981.

-------
unmer, Napier Grass
Summer, Control
2
C
i—I
<£
o
>
-O.
Winter
3
0
12
20
TII-E, days
Figure 7-L9- Comparison of evapotranspiration
from bench scale units. A and B
shov the effect of a hign transpiring
plant (napier grass); B and G show
little effect from season in empty
ur.its.

-------
approximately the same as from healthy plant evapotranspiratlon. This is
perhaps an influence of the large increase in water surface area provided by
the extensive capillary action in the nutrient film root system. Evapo-
transpiration rates for the small scale experiments ranged from 1.2£/m2 for
lake sedge in February to 17.4&/m -d for cattails in April.
Uptake of phosphorus by napier grass at the lower temperature and growth
conditions shown in Figure 7.50 are compared to the higher uptakes in warmer
temperatures in Figure 7.51. Background phosphorus control concentrations
ranged from 12 to 15 mg/JI. Six mg/l were taken up by the napier grass over
a period of eight days to a solution level of about 6 mg/l in cold tempera-
tures. Higher uptake took place in warmer temperatures and phosphorus con-
centrations decreased in the napier grass unit to close to zero in a smaller
period of time under the higher growth rate conditions. It should be noted
that the uptake in the control may have been a result of the attached micro-
algae growing in the control unit.
The results for the nitrogen uptake experiments were less clear perhaps due
to nitrogen loss through other mechanisms. In several of the experiments
the pH approached 8, thus encouraging the loss of nitrogen through volatili-
zation of ammonia. Example of nitrogen uptake is shown in the nitrogen loss
in the control. This is contrasted to the nitrogen uptake in phragmites in
January, 1981.
In some cases the nitrogen uptake in these tests indicated a rapid uptake
upon initial contact with the growing plant system. Figure 7.52 compares
the control for experiments conducted in March of 1981, with initial nitro-
gen concentration of approximately 50 mg/l, and immediate uptakes (within
the first 24 hours) of 20 to 25 mg/l as shown for phragmites, bulrushes and
cattails. It would appear in many cases when the plants were healthy the
nutrient concentrations could be reduced to values approaching zero. The
long-term impact on plants of intermittently increasing the strength of the
solution (or wastewater), however, is not known. Growth characteristics
under intermittent loadings at these low nutrient concentrations are also
unknown.
In these experiments cadmium was also difficult to follow because the remo-
val rates were rapid, with effluents of approximately 0.02 resulting from an
influent concentration of 0.2 to 0.3 mg/£. The results, however, were
highly variable, and the microalgal film that grew in the controls was also
effective in removing the cadmium from some of these experiments.
C.9. Domestic Sewage, Bench Scale Test
Experiments with domestic sewage, similar to those recorded above, were con-
ducted in bench scale units in the Hanover, New Hampshire, greenhouse. A
varied flow experiment with applications ranging from 20 to 187 cm/day
resulted in the removal rates shown in Figure 7.53. This indicates that
there appears to be an optimum area loading rate for both BOD and total
suspended solids which occurs above 50 cm/day. The interpretation of this
data indicates that the removal rate is substrate limited up to the 100
cm/day loading. Above a certain maximum application rate the pollutant
207

-------
+ Control
X Napier Grass
16
DAY OF EXPERIMENT
Fi gur e 7 o 0.
Effluent phosphorus concentrations from
the control and napier grass units in an
early bench scale experiment using a
recirculating solution. The experiment
began on February 27, 1981 •
20
X Napier Grass
CT
o
6
10
u
0
o
DAY OF EXPERIMENT
Figure 7-51. Effluent phosphorus concentrations from
the control and napier grass 'units in
a later bench scale experiment using
a recirculating solution. The experiment
began on April 8, 1931.
208

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Bulrush
Control
Phragmite
Cattail
DAY OK EXPERIMENT
Figure	Effluent total nitrogen concentrations of synthetic sewage from bench scale units
containing .several plant species. This series of experiments began March "18, I98I.

-------
• BOD
ICO	150
AREA LOADING RATE, cm/d
20C
Figure 7-53.
Relationship between area loading
rate and removal of solids and
BOD in bench scale NFT systems
using synthetic sevrage.
21C

-------
(organic matter or the suspended solids) moves through the system so	rapidly
that there is little potential for the removal mechanisms to operate	effic-
iently. The generation of more data in small scale experiments such	as this
would be useful to support this optimization of SS and BOD removals.
A comparison of BOD concentrations in the control unit and those of applica-
tion rates of 20, 148, 187 cm/day is illustrated in Figure 7.54. This shows
that rapid, efficient BOD removal is difficult to achieve at high loading
rates.
Due to the rapid growth of the microalgae film in the sewage substrate, par-
ticularly in the control unit, the results achieved with the small-scale
experiments were of limited value. An example of the effluent BOD concen-
tration in the control as compared to those achieved in several other spe-
cies units is shown in Figure 7.55. Over 200 mg/£ of BOD were removed in
less than 24 hours from the 7.5£ reservoirs, and the residual BOD of less
than 20 mgll was obtained in all units including the control after four days
or less.
C.10. Testing of Optimized Design
Examination of the data midway through the testing program clearly indicated
that the pollutants such as BOD and suspended solids were removed by mecha-
nisms much different than the nutrient removal mechanisms. Changing the
loading rates and methods of application was one obvious way of optimizing
the NFT treatment system. It was felt that if the wastewater could be added
rapidly, a quiescent laminar condition would be created, and then unloading
could be achieved with a slow laminar flow, suspended solids removal would
be maximized and BOD removal might be enhanced. Therefore, a new experimen-
tal setup was designed and tested on domestic sewage in a community nearby
Cornell University. This unit consisted of three separate species as des-
cribed in the Materials and Methods chapter.
The batch-wise addition-removal of domestic sewage (as described in the
Materials and Methods section) experiments modification used at the Cayuga
Heights sewage treatment plant were conducted between August, 1981 and Octo-
ber, 1981. These experiments were highly successful, albeit limited in
scope due to the time required to acclimate the systems and due to adverse
weather in early winter. Note that these were the only experiments where
raw unsettled sewage was used as the feed. Steady state condition
information for the two loading rates tested, 20 cm/d and 30 cm/d, are given
in Table 7.6. The treatment efficiencies for organics and suspended solids
were surprisingly good. Effluent suspended solids were always less than 29
mg/£, and often less than 15 mgfl at the higher loading rate. Total COD
reductions averaged nearly 80 percent.
The improvement in effluent quality between the 20 cm/d and 30 cm/d tests is
most likely related to system acclimation and microbial mass accumulations.
Samples for the latter loading condition were taken after the system had
matured for several weeks of operation at the higher loading rate.
Settleable solids in the effluent, measured in the Imhoff settling cone,
were consistently negligible compared to the suspended solids.
211

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2")0
225
200
175
150
125
100
75
50
25
0
225
200
17';
150
12';
100
75
50
25
0
Control
i'i8 cm/d
/
20 cm/d
J	L
J	L
_L
±
187 cm/d
-L
) 2 1+
1.0 1.2 lU 16 18 0 2 h
DAY OF FXPUKJMENT
10 12
lit 16 10
"igurc 7« • blfi' lucnt biochemical oxygen demand c one ent rat ions of units at different loading
rates in the varied flow experiment using domestic sewage. The experiment
started on June 22, 1981.

-------
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M
LaJ
bfi
a
Q
i'1
Q
W
O
>h
X
O
$
u
M
£3
o
o
ni
150
IK
120
]0'?
90
75
60
U5
30
15
0
135
J.20
105
90
r>
60
30
Control
Bulrush
X
Canary Grass
-O	~
Phragmi tes
-L
J	L
0 1 2 3 ii 5 6 7 8 9 0 1 2 3 U 5 6 7 8 9
DAY OF EXPERIMENT
Figure 7.55. biochemical oxyf.eti demand concentrations over time in recirculating domestic sewage
units containing riif'l'erenl plant species.

-------
TABLE 7.6. SUMMARY OF OPTIMIZED OPERATION OF THE NFT AS A PRIMARY AND
SECONDARY COMBINED TREATMENT SYSTEM USING INTERMITTENTLY
LOADED AND UNLOADED PLANTS. EXPERIMENTS WERE CONDUCTED AT
THE CAYUGA HEIGHTS VILLAGE TREATMENT FACILITY.
Area	Parameter Number	Influent Quality	Effluent Quality
Loading	of		 	
Rate	Samples	Mean High Low Mean High Low
cm/day		 mg/* 	
20
Soluble COD
12
88
124
71
83
93
62

Total COD
12
283
358
168
116
148
93

Suspended Solids
4
125
226
68
43
105
15

Total Solids
1
680
680
680
590
590
590

Settleable Solids
4
7.8
14.2
2.1
<0.1
<0.1
<0.1

NH^N
6
29
32
27
7
13
<1

Organic-N
6
12
16
8
4
10
<1

TKN
6
41
48
35
11
15
5
30
Soluble COD
14
107
176
45
44
59
30

Total COD
12
317
542
193
72
92
33

Suspended Solids
9
139
299
71
15
29
5

Total Solids
5
610
690
570
540
570
500

Settleable Solids
7
4.0
12.0
0.7
<0.1
<0.1
<0.1

nh4~n
4
31
32
29
12
12
11

Organic N
2
9
11
6
5
5
5

TKN
2
40
43
35
17
17
16
*A11 units are mgfl except those for settleable solids, which are
ml/1000 ml.
Based on the limited data, typical results indicated complete removal of
settleable solids, total COD conversions of approximately 75-80%, soluble
COD conversions averaging around 60%, and suspended solids removals on the
order of 90%. Further testing of this optimized treatment sequence with raw
sewage is strongly recommended.
Total nitrogen removals of 75 percent at a 20 cm/d loading rate, and nearly
60 percent at 30 cm/d, could be significant if the results could be
duplicated in larger scale tests.
As noted earlier, questions remain as to the effect of pumping on the solids
in the system. Relatively little data collection was accomplished before
the experiment terminated. There was also significant scatter in individual
data points, so questions of reliability cannot be addressed based on this
work. To be a viable option, the system would also have to be tested at
loading rates higher than 30 cm/day to insure that area requirements could
be minimized.
2\h

-------
In reviewing the three purposes of this experiment, three results are appa-
rent. First, although testing was only accomplished at a moderate flow
rate, the system did perform well in solids capture and conversion of orga-
nic solids. Second, it was established that the plants tested could cope
well with the conditions imposed upon them. Napier grass performed unex-
pectedly well; bulrush flourished as expected under alternate flooding and
drainage; cattail maintained itself but did not grow as well as the other
plants, possibly due to fluctuating water level and reducing conditions.
The most significant observation in this area was the performance of the
napier grass, which promises excellent opportunities for taking advantage of
capabilities to filter solids and convert nutrients. Third, this system
appears to have potential as a pretreatment unit due to solids treatment
capability demonstrated in the study.
Taking the average suspended solids values from Table 7.5 and daily volume-
tric wastewater loading from Table 7.4, the conversion efficiency for sus-
pended solids is computed as 38.4 gm SS treated/m -day at 30 cm/day. This
compares favorably with 16.4 gm SS treated/m^-day at 20 cm/day, the highest
loading rate tested with sewage in New Hampshire. COD conversion and nitro-
gen uptake data also point to the possible refinement of this technique to
replace some conventional primary and secondary treatment processes, but
much more data are needed to confirm system reliability. Heavier loadings
must also be treated in order to achieve an optimized process design.
D. WATER BALANCE AND ENERGY TRANSFER
This section contains empirical data on water losses in an NFT system and a
theoretical analysis of the potential for losses and the possibility for
water recovery.
Evapotranspiration data from a pilot scale control unit at Cornell are sum-
marized in Table 7.7 for the pilot scale units for applications to reed
canary grass. Mean monthly evapotranspiration in the 9.3 m size pilot
units ranged from 2 to 6 £/m2/day and had maximum values approaching
125,000 i/ha/day. Table 7.8 compares the losses in a 9.3 m pilot unit con-
taining reed canary grass to those measured in the recirculation tests
(bench scale units) with synthetic wastewater. This information illustrates
the effects of the plant NFT system on ET as compared to an open water
control where the flow rates were exactly the same and also contrasts the
larger NFT units to the smaller units. Several interesting comparisons can
be made with this information. The smaller units appeared to have
significantly higher water losses than the larger pilot scale units. The
water losses from the control were often around one tenth of that lost from
the small scale unit with mature plant systems. The differences between the
large scale unit and the bench scale units may reflect the uninhibited
movement of air around the water surface and plants. The difference between
the control unit and the NFT unit is more difficult to explain. The
significant increase in ET from the NFT units with plants over a control
unit suggests that the increased surface area available for water
evaporation in the leaf surface can increase water losses. During the
warmer periods of the year, reed canary grass had losses approaching 150,000
i./ha-day, values that appear to be higher than those reported by others.
215

-------
TABLE 7.7. EVAPOTRANSPIRATION OF THE 9.3 m2 REED CANARY GRASS UNIT OF THE
CORNELL BRACEHOUSE. DATA WERE COLLECTED DURING THE TESTING OF
THE 27.9 m2 MDLTISPECIES SYSTEM IN THE SAME GREENHOUSE IN 1981.
Dates
From
To
Average
Evapotranspiration
A/ha-d
Standard
Deviation
1/ha-d
Number of
Observations
2-19
2-28
21500
9500
12
3-1
3-31
24700
9600
30
4-1
4-30
39100
23300
28
5-1
5-31
58100
26900
25
6-1
6-30
45900
21300
28
7-1
7-31
56900
22300
31
8-1
8-16
56100
26100
15
This trend was also observed for the domestic sewage data, as shown In
Table 7.9. Average losses of water from the sewage systems approached
100,000 &/ha-day, and the difference between the controls and the various
plants was also significant, ranging from double to ten times the control
amount. Losses in these small units, however, did not approach the high
values achieved with the Cornell test units.
Although this information appears to be encouraging in terms of recovery of
high quality water in the form of evapotranspiration, it should be kept in
mind that the ET losses represent huge energy conversions. For example, the
loss of 15,000 Ifha-day, if recovered, and requiring an energy investment,
would cost approximately $400/ha-day. Reasons for this are discussed in the
following energy balance and water balance sections.
A design project was undertaken to evaluate the feasibility of recovering
water and energy from ET. This section presents a short overview of the
quantities of water vapor which are lost from NFT plant systems. Two reco-
very systems which were designed for conditions in Ithaca, New York, are
evaluated; and the technical and economic feasibility of these two recovery
systems are discussed. The material is presented in more detail in another
report (Pompe, 1983).
D.l. ET Rates
Results of experiments measuring ET rates from the NFT wastewater treatment
systems were available only at the conclusion of the design project.
Therefore ET rates were predicted based on a model developed for greenhouse
conditions by Lake et al. (1966):
ETP =¦ 0.38 R* + 0.17(* - I.) - 0.17	(7.1)
n	ad
216

-------
TABLE 7.8. COMPARISON OF EVAPOTRANSPIRATION BETWEEN A CANARY GRASS PILOT SCALE UNIT AND BENCH SCALE
UNITS CONTAINING DIFFERENT PLANT SPECIES AT CORNELL USING NUTRIENT SOLUTION OR SYNTHETIC
SEWAGE. ALL ET MEASUREMENTS ARE REPORTED AS A/ha~d. ALL EXPERIMENTS WERE IN 1981.
Pilot Unit ET		Bench Scale ET	
	Unit	
1	2	3	4	5	6
Average
	 Std. Dev. 	
N
Species
2-27
3-12
22500
11600
46500
48400
101000
102000
86500


4500
9000
12900
12300
31600
21300
26500


14
14
12
12
12
12
12



Control
Millet
Lake Sedge
Canary Grass
Soft Rush
Napier Grass
3-18
4-2
30100
19400
65200
49700
115000
69700
84500


13400
20000
24500
19400
51600
29700
20600


15
16
16
16
12
16
16



Control
Cucumber
Phragmites
Canary Grass
Bulrush
Cattail
4-8
4-23
38800
20600
87700
67100
142000
76100
174000


22600
14800
31600
42600
56100
63900
72300


16
11
12
12
12
15
12



Control
Millet
Rose
Canary Grass
Phragmites
Napier Grass
4-28
5-11
45900
45200
98100
111000
38700
36100
63200


24600
30300
32300
33500
36100
9000
30300


11
13
13
13
13
13
13



Control
Cucumber
Canary Grass
Bulrush
Cattail
Phragmites
5-14
5-21
55600
31000
43900
42600
50300
43900
42600


21200
19400
4500
23900
27100
18100
13500


6
6
Control*
6
6
6
6
6



120 MEQ/*
60 MEQ/A
30 MEQ/ft
15 MEQ/£
0 MEQ/t
^This experiment tested salt concentrations; unit 1 was empty, while units 2 through 6 contained canary
grass.
Dates
From To	Average
Std. Dev.
N

-------
TABLE 7.9. COMPARISON OF EVAPOTRANSPIRATION BETWEEN A 27.9 m2 PILOT SCALE SYSTEM AND BENCH SCALE UNITS
CONTAINING DIFFERENT PLANT SPECIES USING DOMESTIC SEWAGE. ALL ET MEASUREMENTS ARE REPORTED
AS £/ha~d. ALL EXPERIMENTS WERE IN 1981.
Dates
From To
Pilot System ET


Bench Scale ET


Average
Std. Dev.
N



Unit


1
2
3
4
Average
Cf/i Hdh
5
6



N
Species


3-23 4-5
83500
14800
78700
98100
32300
30300
51600

127000
21300
61300
56800
15500
31600
36800

4
13
13
12
12
13
13


Control
Cucumber
Canary Grass
Phragmltes
Bulrush
Cattail
4-17 4-30
96800
29000
54200
84500
27700
66500
44500

49800
38700
32900
41900
20600
56100
40600

11
13
13
13
11
13
11


Control
Rose
Canary Grass
Phragmltes
Bulrush
Cattail
5-8 5-21
93200
7100
26500
54200
43900
49000
95400

34100
11600
19400
39400
36800
43200
73500

14
12
13
13
13
13
13


Control
Bulrush
Canary Grass
Phragmltes
Cattail
Napier Grass
5-22 6-4
107000
32300
60000
80000
34200
66500
71000

0
16100
24500
31600
16100
41900
22600

1
13
13
13
12
7
11


Control
Tomato
Canary Grass
Bulrush
Phragmltes
Cattail

-------
where ETP = potential evapotranspiration,1 mm H20/day
1	2
Rq = net solar radiation Inside the greenhouse, mm H20/day
I ™ saturation vapor pressure, mm Hg
&
I, « actual vapor pressure at the outside air at 0900 hr, mm Hg
a
The design of the recovery systems was based on potential ET rates. Actual
rates were assumed to be close to potential rates.
ETP rates are a function of solar radiation and were predicted for January
and July conditions to be 4,100 and 26,200 £/ha-d, respectively. Total
yearly ETP losses were computed through integration after assuming a sig-
moidal shape for the ET curve over the year (which matches the solar radi-
ation variation). They were calculated to be 5.6 x 10 4/ha-yr.
The actual ET rates which were measured in the NFT wastewater treatment
units fluctuated sharply from day to day. Average rates from the large
experimental systems ranged from 21,500 to 56,000 ifha-d for February and
July, respectively. The predicted ETP rates were lower than these values,
but were In the same order of magnitude. The effect of the higher actual ET
rates In the feasibility of the recovery systems is discussed in the later
sections.
D.2. ET Recovery Systems
Various approaches can be used to remove water vapor from air. Exposure of
the air to a surface which has a temperature lower than the dewpoint temper-
ature of the air is one possibility (ASHRAE, 1981). The technical feasibil-
ities of using an air-to-air heat exchanger and that of using a refrigera-
tive dehumldification system with a compression cycle are evaluated in this
section.
The capacity of both systems is a nonlinear function of the size of the
greenhouse. The size of the greenhouse needs to be known for the design.
The systems wer| evaluated for a greenhouse with a floor area of 200 m , a
volume of 890 m and a cover area of 440 m . The total amounts of ET water
vapor for this size greenhouse were calculated to be 82 and 524 1/ day for
Potential evapotranspiration is defined as the ET from a crop which covers
the soil completely and which water supply is not limiting (Decker, 1967).
2
Solar radiation can be expressed in mm H2 0/day by multiplying the radiant
energy by its latent heat equivalent for water. The latent heat of water^at
20°C and 1 atmosphere equals 2453 kJ/kg. The evaporating power of 1 MJ/m
is then 0.408 mm H2O.
219

-------
January and July conditions. They were assumed to be produced during 12
hours per day. The instantaneous ET rates for these months were computed to
be 1.9 x 10" and 12.1 x 10" kg H20/sec, respectively.
An energy balance was constructed around the hypothetical 200 m green-
house. The results for typical January and July days in Ithaca, New York,
are show^ in Figure 7.56. A net energy loss for the whole greenhouse of
4.4 x 10 MJ/d was computed for January, while in July an excess of 433 MJ/d
of energy was calculated.
D.2.a. Necessary cooling capacity
The cooling capacity which is necessary to condense the ET water vapor was
computed based on the following two equations (ASHRAE, 1981).
Equation 2 calculates the relationship between the required humidity ratio
of air and the mass flow rate of air, while Equation 3 computes the neces-
sary rate of heat transfer:
W2 - Wi - ET/ma	(7.2)
q = m^'(h^ - h2)-(Wj - V^h^}	(7.3)
where
W = humidity ratio of air, kg H20/kg dry air
ET =• amount of evapotranspiration for the whole greenhouse, kg H2 0/sec
M = mass flow rate of air, kg/sec
a
q = rate of heat transfer, kW
h = enthalpy, kJ/kg
and the subscripts are defined as
1	= entering air
2	¦ exiting air
a = air
w2 = exiting water
The relationship between the condensation temperature and the mass flow rate
of air for January and July conditions is shown in Figure 7.57, and this
figure also shows the required cooling capacity for mass flow rates at given
temperatures. The designs for the two recovery systems were based on this
figure. The necessary rate of heat transfer at a given mass flow rate of
air would be larger in Figure 7.57 if the measured ET rates were used to
construct the figure instead of the lower computed rates.
D.2.b. Air-to-air heat exchanger—
A counterflow air-to-air heat exchanger which uses colder outside air to
condense the water vapor from the greenhouse air was evaluated. The
configuration is shown in Figure 7.58. Outside air is forced through the
inside tube into the greenhouse. The warm greenhouse air is forced between
the two tubes and is vented to the outside. The whole system would be built
220

-------
Direct, and Scattered
Had i at ion
)|020 MJ/d
Convection
r;7 MJ/d
1020 M.T/d
2U10 MJ/d
Net Energy
1+33 MJ/d
Net Energy
510 MJ/d
17.5°C
FT
H
MJ/d
20J MJ/d
Conduc-
tion
Conduction
Vent i I ation
Vorit.i I at ion
103 MJ/d
figure 7.'.36. Schematic energy balance I'or a hypothetical greenhouse in January (A) and July (ii) in Ithaca,
New York. Greenhouse measures 10 x 20 m arid in covered by a double layer of polyethylene.

-------
Jul;
<
July
<
hH
O
o
o
>-
"V
<
January
10 o
January
1C
1-5
2.0
2.5
.0
0
MASS FLOW RATE OF AIR, kg/s
Figure 7.57- Condensation tenperature and cooling capacity necessary to
recover all ET vacor as a function of mass flow rate of air.
222

-------
Insulation
reen.icuse air
igure fo
7.53.
Configuration of the air-to-air heat
exchanger used in design estimates.

-------
sloped down towards a condensate collection point. The temperature of the
outside air would increase in the direction of its flow as a result of the
absorption of both sensible and latent heat from the exiting greenhouse air.
Meteorological data were obtained from the Department of Atmospheric Sci-
ences at Cornell University, while greenhouse conditions were gathered
through actual measurements. A method which employs the heat exchanger
effectiveness approach was used to determine the dimensions of the heat ex-
changer (ASHRAE, 1981, Kreith and Black 1980). Three variations of the sys-
tem were evaluated, containing 2, 4 and 6 sets of tubes.
For January the lengths of these heat exchangers which would be necessary to
remove all ET vapor were computed to be 196, 143 and 82 m for the systems
with 2, 4 and 6 sets of tubes, respectively. These are much longer than the
20 m greenhouse for which they were designed. They were rejected since they
were not suited for January conditions. If actual measured ET rates had
been used for the design, the system would have been even larger. In July
the temperature of the outside air is equal to the dewpoint temperature of
the greenhouse air. Therefore no condensation of water vapor will occur in
an air-to-air heat exchanger using outside air. This approach was also
deemed infeasible for July condensation.
D.2.c. Refrigerative dehumidification—
A refrigerative dehumidification system with a compression cycle was evalu-
ated as an alternative approach for condensation of the water vapor. Such a
system consists of four basic components: the compressor, the condenser, an
expansion valve, and an evaporator. The energy which is absorbed by the
evaporator and that which is added by the compressor is released by the
condenser (Althouse et al., 1979).
If all water vapor lost through ET were to be recovered, the cooling capa-
city of the system needs to be at least 10.7 kW for January and 50.4 kW for
July (see Figure 7.57). The temperature of the air at the evaporator would
need to be 5°C and 12°C at mass flow rates of air of 0.4 and 1.15 kg/sec for
January and July, respectively.
Systems which can provide these capacities are commercially available. This
approach was considered to be technically feasible. The evaporator was
designed to be inside the greenhouse, while the condensing unit would remain
outside. Ducts would connect the two components. The heat which would be
released by the condensing unit could be recovered by guiding the cooled
greenhouse air (or part of it) through these ducts over the condensing unit
and back into the greenhouse. The CO2 supply necessary for photosynthesis
may be depleted in such a closed greenhouse, and artificial CO2 addition may
be necessary.
The daily energy Input requirements for this ET recovery system were
computed to be 62.4 kWh and 188.0 kWh for the 200 m design greenhouse in
January and July, respectively. The system would produce 82 and 524 Z daily
in these respective months. Some of this energy is lost due to energy
conversion inefficiencies, while some energy is recovered from the heat of
condensation. A mass and energy balance showed that in January the daily
22k

-------
energy input of 62.4 kWh into the 200 m2 greenhouse would produce 82 I/day
of condensate (see Figure 7.59). This would remove 128.4 kWh from the 200
m greenhouse dally, while 159.6 kWh would be released by the condensing
unit. If the air were guided over the condensing unit and back into the
greenhouse, a net increase of 31.2 kWh of energy would be obtained daily.
In July 188.4 kWh of daily energy input to the compressor would produce 524
Jl/day of condensate while removing 604.8 kWh of heat daily.
D.3. Economic Considerations
In the economic evaluation the net present value of the construction costs
and the operation and maintenance costs were taken into account. Only the
compression refrigerative system was evaluated since the air-to-air heat
exchanger proved to be technically infeasible.
Yearly total costs were calculated to amount to $4,615, while the yearly
benefits were estimated at $1,615. The total yearly water recovery from the
ET recovery was predicted to be 111 m^/yr. Water production costs were cal-
culated to be $27/m3. Conventional treatment of wastewater to potable
standards were reported to be $0.31/m3 at the Factory 21 sewage treatment
plant in Orange County, California (Argo and Montes, 1979). Water produc-
tion through ET recovery from NFT wastewater treatment systems would be much
more costly and was rejected as economically feasible at this time.
D.4.	Alternative Approaches for Condensation
Other media could serve as the coolant in a heat exchanger. Cold water
could be used instead of cold air. Water will transfer heat faster than air
does as a result of its much higher density. One naturally available source
for cold water is the sewage influent itself or groundwater. The wastewater
itself may have a low enough temperature to serve as the coolant. Water-
to-air heat exchangers may prove to be more feasible than the air-to-air
heat exchanger examined here. An evaluation of such a heat exchanger is
recommended.
E.	NUTRIENT UPTAKE
Tables 7.10 through 7.14 summarize the dates, yields, dry weights of plant
harvests and the composition of the material as subsampled from the harvest-
ed materials. This information is useful for illustrating the composition
of the material as well as the nutrient and heavy metal compositions that
may occur in the NFT unit treating wastewater. The control that was fed an
inorganic nutrient solution for the duration of the study from 1979 through
1980 is useful for comparing the impact of the synthetic wastewater with
cadmium addition to the reed canary grass. The nitrogen composition for
this plant for the intensive period studied ranged from a maximum of 5.28
percent nitrogen to a low of 3.62. The high concentrations of nitrogen
always occurred during lower productivity periods and low temperature
conditions, and the lower nitrogen contents occurred during higher
productivity. The phosphorus content ranged from 0.40 to 0.70. Reed canary
grass yields were plotted in Figure 7.60 and ranged from 0.36 to 9 g/m /day
of dry matter. The conversion rates of nutrients were calculated based on
the yield data and the elemental concentrations as reported in the tables.
225

-------
J anuary
V?9.6 kWh Condem?^-
Evaporator
120. U kV/h
31.2 kWh
Conversion
TjOsscg
62. U kWh
Compressor
Engine
J ul,y
Engine
Figure Y-'39. Rnergy balance I'or the rcfr iterative compression iiyotcm.

-------
ro
ro
-j
9
8
¦n
i
CM
s
150
9 f
w
Eh 1|
U J
w
i 3
1 -
MAM
HARVEST DATE
!•' i tfuro 7 • 60 • Dry weight harvest y ie.ld oi' a 9-3 m2 rood canary grass control unit.

-------
TABLE 7.10. DATES, YIELDS, DRY WEIGHTS AND NUTRIENT CONTENTS OF AGED
CANARY GRASS HARVESTS IN THE CORNELL BRACEHOUSE UNIT #2.
THIS UNIT WAS GROWN FROM SEED AND USED AS A CONTROL.
Date
Yield
Dry Wt.
N
P
Cd
Dry Weight

g
%
%
%
ug/g
g/m2-d
10/18/79
1072
15.6




10/26/79
274
18.2



0.766
11/1/79
326
11.5



0.672
11/8/79
589
13.5



1.221
11/15/79
--
-



—
11/22/79
1176
14.1



2.547
11/29/79
602
14.1



1.304
12/6/79
149
14.3



0.327
12/13/79
95
13.8



0.201
12/20/79
69
18.5



0.196
1/8/80
39
16.2



0.036
1/17/80
57
19.3



0.131
1/25/80
71
23.0



0.219
1/31/80
66
7.9



0.093
2/8/80
—
—



—
2/14/80
—
—



—
2/21/80
—
—



—
2/28/80
62
24.0



0.229
3/7/80
67
7.4



0.076
3/27/80
506
17.6
5.28
.65
.35
0.479
4/10/80
2050
13.6
5.26
.61
.37
2.141
4/24/80
6850
17.0
4.74
.53
.33
8.944
5/8/80
6776
16.6
4.09
.51
.42
8.639
228
(continued)

-------
TABLE 7.10 (continued). DATES, YIELDS, DRY WEIGHTS AND NUTRIENT CONTENTS
OF AGED CANARY GRASS HARVESTS IN THE CORNELL
BRACEHOUSE UNIT #2. THIS UNIT WAS GROWN FROM
SEED AND USED AS A CONTROL.
Date
Yield
Dry Wt.
N
P
Cd
Dry W|ight

g
%
%
%
Ug/g
g/m -d
5/22/80
5630
14.2
4.74
.56
.51
7.719
6/3/80
4508
15.3
4.89
.50
.52
4.999
6/19/80
3389
16.5
5.14
.56
.38
3.592
7/13/80
1227
13.8
4.09
.50
.52
1.301
7/17/80
958
19.0
4.17
.52
.42
1.398
7/31/80
996
19.8
4.00
.40
.32
1.515
8/14/80
959
20.1
3.62
.49
.41
1.480
8/29/80
895
16.7
4.45
.65
.60
1.071
9/11/80
835
21.9
4.31
.65
.97
1.513
9/26/80
1440
15.1
4.86
.67
.66
1.670
10/9/80
1160
14.5
5.03
.70
.75
1.391
10/23/80
1067
16.3
4.27
.65
.88
1.336
11/10/80
436
17.0
5.03
.65
1.13
.443
229

-------
TABLE 7.11. DATES, YIELDS, DRY WEIGHTS AND NUTRIENTS CONTENTS OF REED
CANARY GRASS HARVESTS IN THE CORNELL BRACE HOUSE UNIT #4.
THIS UNIT WAS THE PRIMARY TEST UNIT IN EARLY EXPERIMENTS AND
SERVED AS THE ET CONTROL IN LATER EXPERIMENTS.
Date	Yield Dry Wt. N	P Cd Dry Weight
g	%	%	% ug/g	g/m2-d
10/8/79
4320



10/18/79
1615
22.4

3.890
10/25/79
440
15.1

1.021
11/1/79
156
15.1

0.362
11/8/79
235
15.1

0.545
11/15/79
137
15.0

0.316
11/22/79
138
17.1

0.362
11/29/79
160
16.8

0.413
12/6/79
171
15.7

0.413
12/13/79
143
16.5

0.362
12/20/79
60
19.0

0.175
1/8/80
90
20.0

0.102
1/17/80
—
—

—
1/25/80
64
20.4

0.175
1/31/80
79
24.0

0.340
2/8/80
—
—

—
2/14/80
69
21.0

0.260
2/21/80
107
19.0

0.312
2/28/80
147
19.0

0.429
3/7/80
251
22.0

0.848
3/20/80
706
17.8
5.62 .61 2.02
1.039
4/3/80
1450
15.6
4.47 .69 .67
1.737
(continued)
230

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TABLE 7.11 (continued). DATES, YIELDS, DRY WEIGHTS AND NUTRIENT
CONTENTS OF REED CANARY GRASS HARVESTS IN THE
CORNELL BRACEHOUSE UNIT U. THIS UNIT WAS THE
PRIMARY TEST UNIT IN EARLY EXPERIMENTS AND
SERVED AS THE ET CONTROL IN LATER EXPERIMENTS.
Date
Yield
Dry Wt.
N
?
Cd
Dry Weight

g
%
%
%
Ug/g
g/m2-d
4/17/80
4912
13.3
4.87
.65
1.00
5.018
5/1/80
7470
14.9
5.01
.63
.63
8.549
5/15/80
4750
14.6
4.47
.56
.94
5.326
5/29/80
3530
18.9



5.124
6/12/80
2695
15.4
4.63
.62
.64
3.188
6/26/80
1142
18.5
4.97
.53
.22
1.623
7/10/80
1823
—
4.41
.58
.74
2.681
7/24/80
1216
19.8
4.52
.53
.29
1.849
8/7/80
1263
17.1
4.35
.67
2.16
1.659
8/21/80
1792
14.9
4.25
.77
.37
2.051
9/8/80
1115
17.7
4.43
.66
6.31
1.179
9/18/80
1749
15.2
5.03
.72
4.37
2.859
10/2/80
2219
14.6
5.50
.73
1.35
2.488
10/16/80
2864
13.3
5.08
.76
4.43
2.926
10/30/80
1232
13.0
4.95
.72
2.73
1.230
11/13/80
288
14.0
6.42
.73
1.62
0.310
12/18/80
272
—



0.131
1/22/81
78
17.4



0.042
3/12/81
1867
16.0
4.91
.68
3.45
0.656
4/17/81
7976
15.9
4.36
.58
2.33
3.788
6/3/81
—
18.6
2.79
.49
2.91
—
7/10/81
7200
25.4
2.45
.33
2.03
5.315
231

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TABLE 7.12. DATES, YIELDS, DRY WEIGHTS AND NUTRIENT CONTENTS OF REED
CANARY GRASS HARVESTS IN THE CORNELL BRACEHOUSE UNIT #3.
THIS UNIT WAS A CONTROL UNIT IN EARLY EXPERIMENTS AND WAS
PART OF 18.6 m2 SYSTEMS LATER.
Date
Yield
Dry Wt.
N
P
Cd
Dry Weight

3
%
%
%
ug/g
g/m2-d
10/6/79
—
—




10/18/79
1370
18.9



2.320
10/26/79
398
17.4



1.064
11/1/79
179
14.2



0.456
11/8/79
787
12.1



1.463
11/15/79
—
—



—
11/22/79
557
10.7



0.915
11/29/79
1197
11.4



2.096
12/6/79
265
13.2



0.537
12/13/79
110
20.6



0.348
12/20/79
69
17.5



0.185
1/8/80
209
20.0



0.237
1/17/80
187
17.7



0.395
1/25/80
1018
16.0



2.189
1/31/80
110
25.0



0.493
2/8/80
—
—



—
2/14/80
80
22.0



0.315
2/21/80
113
19.0



0.330
6/12/80
1840
13.8
4.19
.44
.61
—
6/26/80
4600
17.0
4.97
.53
.22
6.006
7/10/80
3665
—
3.74
.53
.33
5.123
7/24/80
2088
19.4
4.98
.66
1.33
3.111
232
(continued)

-------
TABLE 7.12 (continued). DATES, YIELDS, DRY WEIGHTS AND NUTRIENT
CONTENTS OF REED CANARY GRASS HARVESTS IN THE
CORNELL BRACEHOUSE UNIT #3. THIS UNIT WAS A
CONTROL UNIT IN EARLY EXPERIMENTS AND WAS PART
OF 18.6 m2 SYSTEMS LATER.
Date
Yield
Dry Wt.
N
P
Cd
Dry Weight

g
%
%
%
ug/g
g/m2-d
8/7/80
1703
16.3
4.01
.60
.50
2.132
8/21/80
2541
16.9
4.18
.67
.47
3.298
9/8/80
1568
19.9
4.65
.75
.57
1.864
9/18/80
2085
21.4
3.83
.53
3.89
4.798
10/2/80
1709
16.7
4.53
.56
1.06
2.192
10/16/80
1108
13.9
4.86
.72
1.08
1.183
10/30/80
416
15.3
5.60
.73
2.68
0.489
11/13/80
70
13.2
5.41
.71
1.38
0.071
12/18/80
40
—



—
1/22/81
17
4.2



—
233

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TABLE 7.13. HARVEST DATA FROM THE CRREL GREENHOUSE WHICH GREW CANARY GRASS IN 7.4 m2 UNITS (TOTAL).
Date
Unit
Yield
g
Dry Wt.
%
Dry Yield
g
N
%
P
%
Dry Wt.
g/m2-d
N
Removal
Rate
kg/ha-d
P
Removal
Rate
kg/ha-d
3/3/80
A
1814
14
254
6.0
0.76




B
1588
15
238
5.8
0.77




Total
3402
14
492
(Avg.)
5.9
5.8
0.76
0.76
2.4
1.39
0.18
3/18/80
A
1361
13.5
184
6.1
0.69




B
1588
13
206
5.8
0.80




Total
2949
13
390
(Avg.)
5.9
5.9
0.75
0.75
3.5
2.06
0.27
4/11/80
A
3087
12.5
386
5.9
0.65




B
3522
12.5
440
5.4
0.76




Total
6609
12.5
826
(Avg.)
5.6
5.63
0.71
0.71
4.6
2.61
0.33
(continued)

-------
TABLE 7.13 (continued). HARVEST DATA FROM THE CRREL GREENHOUSE WHICH GREW CANARY GRASS
IN 7.4 m2 UNITS (TOTAL).
Date
Unit
Yield
g
Dry Wt.
%
Dry Yield
g
N
%
P
%
Dry Wt.
g/m2-d
N
Removal
Rate
kg/ha-d
P
Removal
Rate
kg/ha-d
5/1/80
A
3270
12
392
5.4
0.69




B
2720
12
326
5.4
0.74




Total
5990
12
718
5.4
0.71
4.8
2.61
0.34




(Avg.)
5.4
0.71



5/22/80
A
3521
13
458






B
3049
13.5
412






Total
6570
13
870


5.6






(Avg.)
5.4
0.71

(3.01)
(0.40)
6/17/80
A
1848
15.5
286






B
2513
15.5
390






Total
4361
15.5
676


3.5






(Avg.)
5.4
0.71

(1.92)
(0.25)
Parenthesized numbers are estimated.

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TABLE 7.14. HARVEST DATA FROM HANOVER
GREENHOUSE IN 1981.
Date
Species
Wet Wt.
g
Dry Wt.
%
3/20/81
Canary Grass
7878
35.9
4/13/81
Canary Grass
6603
15.5
5/8/81
Canary Grass
8555
14.8
5/14/81
Phragmites
2711
26.5
5/14/81
Phragmites
5412
24.6
6/3/81
Canary Grass
3435
15.9
6/3/81
Phragmites
11232
20.6
^Upper (1.2 m2 cut).
2Lower (1.2 m2 cut).
Some of the nutrient uptake data with the synthetic sewage studies are
illustrated in Table 7.13. A smaller amount of information is available for
the domestic sewage studies. However, this information does illustrate
several comparisons. First, it should be emphasized that none of the
experiments represented optimum yields since the focus was on BOD and
suspended solids removal. No yield information was taken during the Cayuga
Heights sewage studies. Under all high rate loading conditions with the
sewage units large amounts of organic suspended solids were present that
tended to inhibit growth of all species. Subsequent tests should be
conducted using high yielding plants such as napier grass downstream of the
sewage units after the BOD and suspended solids have been removed in the
high loading rate unit. Discussion of the cadmium concentration and other
heavy metals will be included in a subsequent section on toxic constituent
concentrations.
F. PLANT CULTURING AND PROPAGATION FOR ESTABLISHING AND OPERATING AN NFT
SYSTEM
F.l. Culture of Species in Total Controlled Environments
The objective of the cultural task was to identify techniques for rapid
propagation of large quantltltes of the selected species. From these tests,
the essential characteristics of either the propagation system and/or the
plant itself was to provide rapid germination and some protection to the
root from direct sunlight. Sunlight promotes algal growth that inhibits
rapid root development. Experiments conducted by General Electric Company
were in a highly artificial environment (controlled temperature, light
intensity and duration, humidity and carbon dioxide concentration) that
would not be considered for application to wastewater treatment. However,
propagation experiments can be used to approximate less controlled condi-
tions. All yields and nutrient uptakes will be significantly less in con-
ventional greenhouses. All species rooted rapidly when provided with narrow
strips of shade at varying widths (the width depended on the species). Root
236

-------
development was rapid in shade, shoot growth followed, and shading by the
shoot quickly eliminated the algal growth. This sequence held for all
species except the cattails, which were not inhibited by algal growth.
Figure 7.61 indicates the extent of the algal growth on newly germinated
seedlings. Watercress is shown in Figures 7.62 and 7.63 before and after
the sixth harvest.
A subsequent set of trials involved propagation by plugs of the reed canary
grass, coastal Bermuda grass, and watercress. The plugs were separated by
various distances; spacing of up to twice the diameter of the plugs produced
rapid filling of the interspaces (see Figure 7.64). This proved to be a
successful method of propagation.
Napier grass cuttings are shown as received in Figure 7.65. The rapid
response is dramatically Indicated in Figure 7.66 and 7.67. Comparisons of
characteristics of roots of the species are shown in Figures 7.68 to 7.71.
It is clear from these root observations that several of these species may
have a role in wastewater treatment.
The cultural experiment series in the controlled environment greenhouse
succeeded in establishing a crop of each plant species In six weeks or
less. Healthy plants with vigorous and extensive root systems were
maintained through several harvests.
F.l.a. Nutrient Removal in Total Controlled Environmental Greenhouses—
Each of the crop stands was propagated in a low nutrient solution, similar
to a half strength Hoagland's solution (Hoagland and Arnon, 1950). When
active growth was evident the crops were given a full strength nutrient
solution such as is used in hydroponic operations. Once stand establishment
was assured, nutrient levels were reduced to those indicate in Table 7.15.
An example of nutrient disappearance for the baseline species is shown in
Figure 7.72 (see Appendix for other experiments). Exact bed areas and
volumes of recirculating nutrient solution are indicated on the respective
figures. For reed canary grass, napier grass, and watercress in excess of
1 g/m nitrogen disappeared within 24 hours of application (vol = 230 I; 230
x 30 ppm = 6.9 g per 6 m2 bed area). The linearity of the nitrogen removal
down to less than 1 ppm is also apparent. Removal of phosphorous was also
very efficient; the time required for removal was slightly longer. It is
also clear that the species operate with different efficiencies. Reed
canary grass and napier grass were more efficient in the removal of
phosphorous than the other species; bermuda grass efficiency was generally
disappointing.
F.l.b. Biomass Production—
Biomass yield records were maintained since the first, harvests oc the
crops. Computation of dry weight biomass yields is shown in Table 7.16 and
extrapolated to annual productivities.
237

-------
Figure 7.6l. Plant seedlings growing on mat in NFT trough
Darkened areas around roots are algal growth


< Figure 7.62. Growth of watercress before biweekly
harvest. Plants grew to 20 err. above
bed floor.

-------
Figure 7.63- Sxand cf v/atercress after biweekly harvest.

Figure 7.6'-. Stand of reed canary grass grown from plugs.
Note that, grass has filled in areas between
plugs, thus expanding the root max and
limiting algal production.
239

-------
Figure J.65- Napier grass cutting at initial transplant
into the NFT trough.
Figure J.66. -Napier grass stand at tine .of. .move to
laraer bed.

-------
rigure 7-67- Regrowth of napier grass three weeks after
first harvest.
mes ?
	.«-r		
m, is, m
Figure 7-68. Side view of root mats of reed canary grass,
coastal Bermuda grass, and water cress grown
in NFT trough.
2Ul

-------
3 mw ws&
£. s.'tw* erfi*-* *

PfC.B,W70
Figure J. 69. Bottom view of root, mats of reed canard-
grass, coastal Bermuda grass, and
water cress grown in NFT trough.

ISill
Jill
mpkc mscm
mm
Figure 7- TO. Side view of root mats of cattails and
napisr grass grown in NFT trough.
242

-------
. .. -oot mats of cattails and
gure T.Tl. Botto. «ri;s-rw la „ .rough.
243

-------
IV)
i=-
\"
W
e
V)
h
w
I I
OS
hH
I«1
hJ
§
o
CO
0 14 81;
SAMPLING TIME, hours
Figure ¦( .12. Nitrogen and 'jhosphoru;; removals from General Electric NFT unit
containing re-'d canary grass.

-------
TABLE 7.15. GRASS NUTRIENT FORMULA USED IN PLANT PROPAGATION
IN TOTAL CONTROLLED ENVIRONMENT
Constituent	Nutrient	Concentration (mg/Jl)
Ca(N03)24H20
Calcium
45

Nitrogen
40
kh2po1+
Potassium
12

Phosphorous
10
MgS0il*7H20
Magnesium
5

Sulfur
7
FeDTPA
Iron
0.1
MnS04H20
Manganese
1.0
H3BO3
Boron
0.2
(NHit)6Mo702it4R 20
Molybdenum
0.05
ZnS0i+*7H20
Zinc
0.1
CuS0i+5H20
Copper
0.1
TABLE 7.16. BIOMASS YIELD OF TEST SPECIES SHOOT HARVEST
Species	Yield,	Dry Weight Yield
Wet Wt. Dry Weight 	
kg/m2-wk Fraction kg/m2-wk g/m2-d tonnes/ha-yr
Napier
3.55
.083
.29
41.5
150
Watercress
0.92
.066
.061
8.7
32
Bermuda Grass
0.1 _
.20
.02
2.9
10.4
Reed Canary Grass
0.61
.15
.09
12.8
46.9
Cattails
2.21
.048
,106
15,2
55.3
F.2. Culture of Species in Greenhouses
The response of plants placed in NFT units varied according to the environ-
mental conditions in the greenhouses and the solutions into which they were
placed. The wetland plants and some terrestrial plants were placed in non-
carbonaceous nutrient solution in Ken Po9t greenhouses in the fall of 1980
and are discussed first. Other terrestrial plants were later cultivated in
this clean solution. Many of the wetland plants, along with flower and
vegetable plants, were transported to Hanover and tested on sewage; and
these are discussed later in this section.
F.2.a. Response to Non-Carbonaceous Nutrient Solution—
The plant species placed in the NFT units exhibited a variety of growth
21+5

-------
responses, from practically no growth to luxurious growth. Accordingly, the
species were evaluated on the basis of top growth and root development and
were assigned to one of the following categories:
1.	species that flourished;
2.	species with marginal growth;
3.	species that died or failed to form new growth.
Table 7.17 summarizes the overall responses of different plants to the
nutrient solution. It should be noted that the species were rated based on
growth during the short-day conditions of autumn and winter. If the plants
had been grown throughout the summer months, higher productivity would have
been expected. The value of conducting autumn and winter tests, however,
was that the species experiencing a period of dormancy could be distinguish-
ed from those that grew continuously. It should be made clear that these
listings are based on survival performance in NFT systems. When nutrient
levels are lower than those used in this study, plant performance may be
affected. Similarly at high loadings of sewage, plant response could be
quite different.
Species that Flourished—Among all plant species grown in the units, none
surpassed the vigorous growth of napier grass. Grown from cuttings, this
species rapidly developed a thick root mat and dense top growth. A colony
of the grass may be rapidly increased in size by propagating shoot
cuttings.
Reed canary grass grew very well, even after repeated top growth harvests.
The root mat was well developed. This species is exceedingly common in
wetlands of the Northeast, so wild stock is readily available for
transplanting purposes. Also, the plant was easily established from seed in
the greenhouse.
Although the shoots of phragmites grew well, shoot density and vigor was not
equal to that of napier grass. Roots developed into a relatively thin mat
and new shoots were produced from rhizomes. Following a winter harvest cf
the shoots, the plants at first responded slowly but eventually developed
new shoots from the rhizomes. As day length increased in the spring,
phragmites grew rapidly and developed a more extensive root mat.
Soft rush continuously produced new shoots through the winter months and
developed a thick root mat. Although the plants were relatively low in
stature (<50 cm), shoot density was high. During spring and summer plant
vigor declined until little or no new shoot growth was produced. The
reasons for this plant's condition at that time are unclear.
Lake sedge experienced good growth during autumn but grew slowly during
winter. The root mat was relatively thin. Spring and summer growth was
relatively poor.
Softstem bulrush grew well initially but responded very slowly following a
harvest of the shoots. The shoots of this species, therefore, should not be
harvested until they attain full development.
2k6

-------
TABLE 7.17. PLANT RESPONSE TO NUTRIENT SOLUTIONS
Category	Species
Species that flourished	Napier grass
Reed canary grass
Phragmites
Soft rush
Lake sedge
Softstern bulrush
Roses
Geraniums
Petunias
Species with marginal growth	Japanese millet
Bristly sedge
Cattails
Sugar cane
Eastern cottonwood
Arrow aram
Cucumbers
Carnations
Blackberry
Species that died or failed to grow	Wild rice
Swamp loosestrife
Woolgrass
Lizardtail
Three ornamental species performed well in the NFT units. Roses, geraniums
and petunias showed good top growth, root development and flower
production. These plants were grown under the longer daylengths of late
spring, so their performance in winter light conditions was not
demonstrated; the general response of geraniums and petunias to short days
is elongated stem growth. Roses, however, continued to grow well as long as
the temperature remained above 15°C. Temperature stress in the form of red
leaves was evident in the roses when temperatures dropped below 15°C.
Species with marginal growth—Japanese millet, an annual, established from
seed, grew to maturity, but the plants were stunted (<20 cm). An
established colony later, however, grew nuch more vigorously than the first
colony, suggesting that the plants may have been responding favorably to an
increasing photoperiod. The greatest potential of this species lies in its
ability to grow rapidly from seed during the summer.
Bristly sedge grew slcwly during fall and winter, but did develop a fairly
dense root mat. Growth in this species appeared to be limited by short day
and/or low light intensity conditions. During the summer months it grew
well and produced seed.
21+7

-------
Cattail seedlings and overwintering shoots grew very slowly and seemed to be
in a dormant condition due to the short photoperiod (and/or low light
intensity). With increased day length, top growth improved and a fibrous
root mat developed.
Sugarcane grew very slowly during the winter and did not develop a root
mat. Its growth, however, Improved with increased day length. Also its
placement next to napler grass might have been an impediment, since napler
grass quickly shaded any adjacent plant.
Although some cuttings of eastern cottonwood died, others developed lateral
branches and grew fairly well. The roots were sparse and did not form a
mat.
Arrow arum remained dormant until February when the increasing daylength
and/or light intensity stimulated growth. Later, its leaves grew
vigorously, although the root mat was not very extensive.
Cucumbers and carnations performed moderately well in the clean solution NFT
unit. Like roses, cucumbers seemed subject to nutrient deficiency at higher
levels of nutrients than other plants in these units; conversely, when the
cucumbers grew well they grew as fast as any other plant except napler
grass. Carnations grew continuously in the nutrient solution but did not
produce large, high quality flowers. Stems were often elongated and did not
grow erect, possibly due to shading by adjacent plants.
Rooted cuttings of blackberry bushes were grown in NFT units, but survival
was poor. This was due primarily to the root configuration of the cuttings;
the woody roots grew around the lower six inches of the cutting, and only a
small portion of the roots was in contact with the nutrient film. The
plants that survived the first few weeks grew very well, suggesting that
blackberry bush production in NFT units may be successful.
Species that died or failed to grow—Wild rice seeds planted in NFT units
germinated within a week, but the seedlings were spindly and few attained an
erect stature. All eventually died. A large amount of algae that became
established in the unit may have contributed to this failure. Possibly,
this species would grow better given a longer photoperiod and/or a higher
light intensity.
The rooting branch tips of swamp loosestrife grew slowly, and leaves soon
began to be shed and the shoots died. The leaves were damaged by spider
mites, but it is not known whether this factor contributed to the plant's
demise. Had root masses been collected rather than branch tips, growth may
have been better. The two species of burreed (big burreed and burreed) had
overwintering shoots when they were placed in the NFT units, but did not
grow. Both species were, therefore, considered unsuitable for use in the
NFT units.
Wool grass and lizardtail failed to grow appreciably and appeared to be in a
state of dormancy.
248

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F.2.b. Plant Response to Sewage in NFT Systems—
Plants in the Hanover greenhouse were grown in sewage. Their growth
responses were in many ways similar to the plants grown on nutrient
solution. Overall, however, the sewage loading proved to be a harsher
treatment. The plants were again rated in three categories, from those that
flourished to those that died, and this categorization is presented in Table
7.18. Because the test unit was 36.6 meters long, a plant's position in the
unit may have had some effect on that plant's survival and growth. After
some plants were grown in the NFT unit some were planted in standard potting
mixtures to determine whether plants could either recover from pathologies
suffered in the unit or flourish in pots suitable for market. Plant growth
in sewage and recovery of the plants following stressful periods are
discussed in the following sections.
TABLE 7.18. PLANT RESPONSE TO SEWAGE
Category
Species
Species that flourished
Cattails
Bulrush
Strawflowers
Japanese millet
Roses
Napier grass
Marigolds
Wheat
Phragmites
Species with marginal growth
Bristly sedge
Chrysanthemums
Carnations
Tomatoes
Comf rey
Reed canary grass
Soft rush
Cucumbers
Species that dies or failed to grow
Lantana
Gemanlums
Fuchsia •
Petunias
Species that flourished—Cattails began to grow rapidly after they were
introduced to the NFT unit in February. Figure 7.73 shows cattails as well
as other plants which responded well. Roots and shoots appeared healthy
throughout the testing period and reached heights of 2 meters. They
responded to harvesting well, even when a second harvest was conducted on
the same plants. Cattails which were cut back resumed growth and were
eventually Indistinguishable from those not cut. Although cattails grown in
synthetic wastewater at Cornell flowered profusely, cattails grown in sewage
never flowered.
249

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Figure 7-73.
Cattail, bulrush, phragmites, and
canary grass growing well in the
Bracehouse 36.6 meter system.
250
s

-------
Bulrush exhibited slow growth under low light conditions (February and early
March), but rapid growth began and continued thereafter. Most shoots
reached a meter or longer in height and flowered. Upon harvesting the cut
stems died and were replaced by new growth emerging from the rhizomes. Over
the course of testing, the plants developed a thick stand.
Strawflowers grew very well, producing stems over 60 centimeters tall and
large flower heads. The plants appeared very healthy, showing no signs of
insect damage or root rot.
Japanese millet performed well and showed an interesting phenomenon. Where
a gradient of film depth was present (due to an unlevel unit) the taller
plants were produced on the shallower film areas. Overall the millet grew
rapidly and produced a large quantity of seed, although this species in the
field may grow somewhat taller (Rawinski, 1981). The root system developed
an extensive mat, particularly in the shallower areas.
Roses grew well in the sewage. When flower buds were cut the plants
continued to grow and produce flowers. Rose cuttings in peat pots developed
some adventitious roots projecting from the pots above the film surface (see
Figure 7.74). Roots above the sewage appeared healthier than those below
it. Following a severe infestation of spider mites, olants developed new
leaves and shoots, and resumed growth.
Just as in the nutrient solution, napier grass grew vigorously. A thick
root mat developed rapidly such that the root zone was never completely
submerged although the mat itself raised the water level. Tall stems were
cut in one-node sections and placed in sewage for rooting, which occurred
within two weeks. The cut stems continued growing from branches at the
lower nodes, and these new stems eventually produced inflorescences.
Marigolds flourished in the sewage, putting out new leaves, increasing in
height and flowering. The root system developed well and appeared healthy.
Wheat established a healthy stand 20 centimeters tall in about two weeks
(starting from sprouted seed). The root system was developing into a
fibrous mat.
Phragmites grew slowly in early spring but grew much more rapidly later.
The position of phragmites in the system was changed, which also affected
its vigor. It responded well to cutting, showing a rapid regrowth. Roots
formed a dense mat around the rhizomes and raised the entire plants.
Species with Marginal Growth—Bristly sedge had considerable top growth and
healthy roots. In late spring it went to seed, and leaves turned brown at
the tips. After producing seed new leaves continued to grow while older
ones continued to brown.
Chrysanthemums grew well initially, but at the initiation of flowering the
plants suffered a severe aphid infestation and root death. Peat pots were
removed from the roots and buds were removed from the stems, but the plants
showed little improvement. The buds did not grow and the plants barely
251

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Figure	Root zone of a rose in peat pot showing
anoxic accumulations at bottom.
252

-------
survived. Because this plant has a very strong reaction to photoperiod, it
might have performed much better in the summer.
Carnations were similar to the mums in that their initial growth was good,
but they soon suffered from rotting roots. There was some regrowth after
flower buds were removed. The peat pots were again suspected of causing
problems and were removed, but only small growth in roots was noted.
Initially, the tomatoes showed healthy roots and vegetative growth. As the
plants began flowering and setting fruit, lower leaves began turning brown
and died. Roots were never extensive but in latter stages of fruit produc-
tion they turned gray. The fruit ripened when still very small.
Comfrey wilted when it was first put in the unit but later sent out new
leaves and appeared healthy.
Read canary grass grew very well through the lower loading rates tested (6.9
and 10.2 centimeters per day). Toward the end of the 20.3 centimeter inch
per day loading much of the grass had died and the productivity of the
remaining grass was quite low. Under these conditions, new leaves in the
grass were rare, and much of the root mat was black and rotten. It appeared
that many solids from upper parts of the system had been washed down and
trapped by the canary grass's finer root system. The grass was flushed with
tap water for several days to remove solids, after which the system was
operated at a low 5.1 centimeter per day loading. The grass remained viable
and recovered slowly at this low condition.
Soft rush appeared to be in dormancy until late April. Prior to that time
much of it had degenerated and was discarded as dead. The remaining plants
appeared healthy and had firm white roots.
Cucumbers, when first placed in the unit in February, responded with vigor-
ous growth. Good shoot and root development and flowering with subsequent
fruit were noted. When the loading rate was Increased from 6.9 to 10.2
centimeters per day, roots deteriorated rapidly which was followed by the
degeneration of plant tops (see Figures 7.75 and 7.76). The plants were
removed from the unit when appearing nearly dead. A second set of cucumbers
were introduced to the unit but did equally poorly, and all eventually
died.
Species that Died or Failed to Grow—Lantana flowered immediately upon
placement in the unit but produced only small leaves and a very small root
system.
Geraniums were the first species to show signs of failure. Leaves yellowed
and dropped, and root6 deteriorated quickly. The poorest performance among
geraniums was noted for those planted in the greatest depth of sewage.
Attempts to culture geraniums without peat pots gave the same results.
Plants flowered continuously, but leaves produced were very small. Root
growth or regeneration was minimal.
Fuchsia grew well initially but then degenerated rapidly. Plants lost
leaves with no new production.
253

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Figure.7-75- Hanover units containing cucumbers during
early testing of the large unit. Note
stressful condition of plants.
Figure 7.76. Poor root development of cucumber after a
month in the EFT unit in the Hanover
greenhouse.
2?k

-------
Petunias flowered but never grew in height or put out new roots. Leaves
yellowed and dropped, and roots eventually rotted away.
Plant Order in the NFT Sewage System—Plant species were placed in the NFT
units in the order in which they were judged most suited: first, plants
able to withstand high solids sedimentation and anaerobic root zones;
second, plants capable of rapid nutrient uptake and high biomass production;
and third, plants capable of flourishing on low nutrient concentrations.
The initial setup of the multispecies unit is shown in Figure 7.77.
Cattails and bulrushes were in the first 6.1 meter section followed by other
wetlands plants considered hardy enough to survive the adverse conditions.
Because little was known of the other species' responses to sewage, they
were arranged in the remaining 24.4 meter section on the basis of their
expected nutrient uptake. As more species became available, they were
entered into available spaces judged best suited to them.
The specific placement of plants in the units did have a significant affect
on the performance of some species. Roses grew well when they were placed
in a location 30 to 35 meters downstream of the influent but fared poorly
when placed between 10 and 12 meters. This later section failed to produce
buds and grew slowly. Similarly, tomatoes grew better between 35 and 36
meters than between 20 and 2^ meters.
Some species appeared to grow well regardless of placement. Napier grass
thrived at the 35 to 36 meter location and also at the 12 to 13 meter
section. In fact, napier grass cuttings were propagated in the sewage film
between 13 and 15 meters. Millet also grew well in considerably different
locations, first at 29 to 30 meters and later at 10 to 12 meters. In the
main testing unit, phragmites did not perform well when it was in the first
12 meters of the unit. This part of the unit had only a slight slope and
usually contained standing sewage. Phragmites roots were clogged with
solids and were not growing well. For testing of the second condition, the
phragmites were moved to the 12 to 24 meter position which was further
downstream and had a greater slope. The phragmites roots performed well
there (as did the cattails and bulrush which replaced it in the first 12
meters).
From these observations one could conclude that more species could survive
further from the influent than closer to it. The D.O. profile of this unit
generally supports this. The ability of some plants to grow at various
locations indicates that the organic buildup is not an impediment to plants
in general, but it may have a gradient effect on some species.
One question that was not answered was whether plants which thrived at the
influent (cattails and bulrush) could survive in lower reaches where condi-
tions were more favorable to most plants.
Plants Removed for Potting—Toward the end cf the experimental phase, flower
species were removed from the multispecies unit and potted. This was done
to see whether placing them in a soil medium would improve their vigor or,
if they were growing well, to find out if they could be potted for
transport. Six flower species were selected. Of these, carnations,
255

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affluent
36 .¦? m
Napier Grass
(Tomatoes)
Roses
Fuchsia
(Marigolds M-
Carnations
(Petunia) J (Wheat)
Millet
c+
H-
C\
Cc
Figure 7-77. Schenatic diagram of second system in Hanover
greenhouse shoving locations of plants relative
to sewage flow. Distances shown are lengths from
sewage influent. Plants in parentheses were
introduced later in the experimental program.
256

-------
chrysanthemums, geraniums and lantana improved their vigor after repotting,
looked much healthier and increased their growth rate. The two species
which showed no significant improvement after potting were roses and
fuchsia.
F.3. Diagnosing and Treating Plant Growth Problems
Because reed canary grass was the most frequently used test species, most
experience was obtained in diagnosing and treating problems associated with
the growth of this species. In the Brace house the plant was grown continu-
ously for two years, during which time it suffered several infestations.
The major pathologies and treatments are shown in Table 7.19. Aphids
flourished periodically, probably because of the absence of naturally occur-
ring predators. Fungus infestations in turn developed on the sugary "honey
dew" secretions of the aphids. There developed a large population of
spiders, however, which heavily fed upon the aphids and flies (Psychoda),
which abounded in the greenhouse.
TABLE 7.19. REED CANARY GRASS PATHOLOGIES AND TREATMENTS
Problem
Treatment
Major Results
Minor Results
Algae clogging
seedling roots
and increasing pH
Aphid infestations
and secondary
fungal attack
Aphid infestation
Fungal infection
(Pythium sp.)
Fe deficiency
0.2 mg/1 CuSOt*
in recirculated
solution
Chemical
oastieidea
by
iaay beetle
introduction
Specific
fungicides
Subdue and
Bayleton used
Sequestrene
chelated Fe
added
Algae growth
cut back; grass
outgrew algae
Aphids wiped out;
fungus infection
reduced
Aphids controlled;
few adult bettles
noted; control
may also be due
to spiders or
parasites
Drought symptoms
remedied
None; solution
replaced after
3 days
Grass matted
down by heavy
spray
None
Yellow appearance
disappeared
Testing suspended
during treatment;
effects on micro-
flora unknown but
may be major
Plants less
susceptible to
fungus attack
The atmosphere In the Brace house had a high relative humidity due to the
constant operation of the NFT units. This high humidity was relieved only
257

-------
Figure 7.78. Black root zone below dead reed canary grass.

Figure 7-79. Geranium plant showing new root growth
8-x ter old roots had routed in peat, pot-.
258

-------
Fig-ore 7.80. Chrysanthemum and peat pot shoving absence
of roots due to root death.
259

-------
by ventilation used for cooling. High temperatures coupled with this high
humidity provided excellent conditions for fungus development. Infection of
a Pythium species occurred several times. This disease caused the death of
the more mature grass leaves. These infestations appeared to be prevalent
during periods when the grass was stressed. Aphlds were commonly noted on
grass leaves when the units were operated at high hydraulic loading rates
which resulted in anaerobic deposits among the roots. Pythium infection was
noted during a period of nutrient deficiency in the feed solution.
In the sewage loaded system the canary grass grew well until higher loading
rates (20 centimeters per day) were tested. Solids then accumulated among
the roots and produced an anaerobic black colored mass (see Figure 7.78).
Grass roots died and plants grew poorly until the deposits were removed by
flushing with fresh water. The grass soon thereafter recovered.
Other plants suffered similar Infestations. Weakened plants were
susceptible to pest attack and became hosts to the rapidly reproducing
disease organism. Before long the spread of the disease to nearby healthy
plants would be noted. Growing several species in the same greenhouse
requires close observation and timely action to control pests. In
commercial greenhouses this problem is overcome by limiting a particular
greenhouse to a single species, which allows the climate to be tailored to
the plant.
General root death affected many species when they were grown in sewage.
These species Included geraniums, cucumbers, chrysanthemums, fuchsias,
carnations, petunias, tomatoes and lantana. Figure 7.79 shows a geranium
partially recovering from root death, and Figure 7.80 shows a similar
condition in a chrysanthemum. It was hypothesized that some of this root
death was due to waterlogging by the peat pots. Removal of the peat
material from the roots, however, resulted in no improvement. Along with
the root death, fungus gnat larvae appeared in the sewage test units and
grew to large populations (see Figure 7.81). A solution of dlazanon was
used to soak the root zone of all the plants downstream of any known
occurrence of the fungus gnats. Some improvement was noted in plants
following this treatment and the removal of peat pots, but the fungus gnats
were not eliminated from the system.
To ensure that atmospheric gases had maximal exposure to the sewage, the
placement of black plastic, used to inhibit algae growth, was elevated in
the units so that It was not in contact with the sewage.
White flies Infested cucumbers, fuchsias, tomatoes and lantana. These
insects often pose a serious problem in commercial production of these
crops. They were treated with Resmethrln spray and controlled, but not
eradicated. Periodic treatments were necessary.
Aphlds infested many plants but were particularly severe on chrysanthemums,
bulrushes and cattails. In the sewage system, the general treatment for
aphlds was fumigation with a nicotine bomb. This treatment was effective in
the short term, but needed to be repeated periodically.
260

-------
Figure 7*81. Larval gnats in connecting gutter between
trays at the Hanover greenhouse.
2ol

-------
Spider mites first infected roses and were treated with rose dust. At their
peak they infested many plants such as cattails, cucumbers, blackberries,
bulrush, etc. Pentac was used as the general spider mite treatment. Again
the treatment had to be repeated at regular intervals.
G.	TOXIC CONSTITUENT CONSIDERATIONS—CADMIUM AND TOXIC ORGANICS (VOLATILE
AND NONVOLATILE)
The data presented earlier for the composition of canary grass species in
Tables 7.10 through 7.14 and Figure 7.82 showing the example decrease in
cadmium are useful in describing the fate of these elements in wastewater.
Most of the cadmium data and the application rates that were used to various
systems are shown in Table 7.20. This table confirms the rapid uptake of
cadmium. The influent concentrations of 0.1 to 0.3 mg/£ represent a waste-
water that is relatively contaminated with an industrial waste that
generates significant quantities of cadmium. These values decreased signi-
ficantly in the effluent down to low values of around 0.01 and 0.02 and 0.04
in most cases. Domestic sewage often contains values of .01 mg/£ cadmium.
Example cadmium removal rates are shown in Figure 7.82.
The data shown in Table 7.11 and 7.12 Indicate that the canary grass had
significant concentrations of cadmium in the plant tissue. An effort to
illustrate the mass flow of cadmium through the system is shown in Table
7.20. The cadmium uptake in the plants appeared to be an insignificant
portion of that applied. This indicates that since in many cases most of
the cadmium was removed, the larger majority of the cadmium is assimilated
and held In the root zone.
Due2to the high concentration of microbes (entrapped mass greater than 1,000
g/m ) In the large root surface area, it was hypothesized that the NFT
system should have some active pollutant removal mechanisms for some of the
toxic organics. Experiments were conducted by adding small amounts of
background toxic organics by Jenkins of the CRREL staff. A summary of the
fate of the volatile and nonvolatile organics is shown in Table 7.21. This
information Indicates that the 36 meter long units were effective in
removing most of the applied volatile organics and nonvolatile organics,
excepting for dlethylphthalate. In all cases the PCB's that were added to
the system were removed by greater than 90 percent even at high concentra-
tions. This Information suggests that the NFT roughing treatment system
could also be significant in removing the toxic organics that will be
contained in many wastewaters.
H.	COMPARISON OF SYNTHETIC AND DOMESTIC SEWAGE IN NFT SYSTEMS
The general approach of this study was to attempt to operate synthetic
wastewater under comparable loading conditions as the tests with the
domestic sewage. The goal was to show that the results that were obtained
with a synthetic wastewater could be extrapolated to sewage so that future
testing could be simplified at small scale with a synthetic wastewater. The
data shown in Tables 6.11 (characteristics of influent synthetic and
domestic sewage) and Figure 7.83 indicate that there was a good correlation
between a number of the variables in synthetic wastewater and domestic
262

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TABLE 7.20. FATE OF CADMIUM IN NFT PILOT SYSTEMS AT CORNELL
Area System Number Cd
Cd
Cd
Cd
Dates
Loading Sije of Applied Effluent In plants	in R£G
Cm/d m Units mg/m -d mg/m -d ng/m -d	mg/m -d
(Univ. No.)
Cd
in Phr^g.
mg/m -d
(Univ. No.)
Cd
in Cu^es
rog/m -d
( Uni v. No.
Cd
in Cattail-
Bulrush
mg/m -d
) (Univ. No.)
Cd
Control RCG
mg/m ~d
3/18/80 -
5/11/80
5.1
9.3
1
16.80
2.55
3.42xl0~3
(±2.10x10 )
3.42xlO~3
(1)
	 	 	 2/14X10-3
5/12/80 -
6/17/80
10.2
9.3
1
21.42
6.12
3.53xl0~3
(±2.10x10 )
3.53xlO-3
(1)
	 	 	 2.63x10-3
6/18/80 -
7/ 3/80
5.1
9.3
1
7.65
3.06
0.36
(±0.36x10 )
0.36xlO-3
(1)
	 	 	 1.02xl0-3
7/ 4/80 -
9/ 8/80
20.3
9.3
1
30.45
18.27
2.86xl0-3
(±2.83x10 )
2.86xl0-3
(1)
	 	 	 0.58x10-3
9/ 9/80 -
10/15/80
10.2
18.6
2
19.38
6.12
7.46xl0~3
(±6.57xl0-3)
7.46xl0-3
(1&2)
	 	 	 1.20x10-3
10/16/80 -
10/31/80
20.3
18.6
2
50.75
22.33
4.73xlO~3
(±5.57x10 )
4.73xlO-3
(1&2)
	 	 	 1.18xl0~3
2/13/81 -
3/14/81
6.9
27.9
3
2.07
0.21
	
0.01
(3)
	 	 	 4. 6x10-4
4/16/81 -
5/10/81
10.2
27.9
3
18.36
5.10
	
4.4xl0~3
(3)
	 	 	 1. 7xl0-3
5/11/81 -
6/10/81
20.3
27.9
3
12.18
4.12
	
	
	 	 	 	

-------
JO
o
i—t
3
0.2
3.1
<
u
R = Canary Grass
B = Cattail/ Bulrush
P = Phragmites
DISTANCE FROM INFLUENT, x
.gure 7-82. Cadmium concentratior.s at various points
in three different length systems, the
longest of which contained several
species. All curves from 10.2 cra/d
loading conditions.

-------
Date
J/10/811
J/12/81
4/I5/M1
4/24/81
TABLE 7.21. TRACK ORCANIC CONCENTRATIONS IN SEWACE IN THE HANOVER NPT SYSTK*.
(Value* In pph)
vulaillc Trace Organic^
CHLOROFORM
Applied
TKTRACHLOROETHYLENE
TOLUENE
Applied	Effluent from	Ann HeJ Effluent from	Applied Effluent from
First Second Third	First Second Third	First Second Third	First Second Third
U X	03 X	94 X
65. 9	28.0 6. 5 J
J1.2	9.86 5.05
10.b	4.92 2.14
100 X	0 X 61 Z 97 X 99 X 0 X 76 X
1.17	12.2	3.6* .533	.118	29.9 2.18 .219
1.84	9.83 3.U .880	.35*
1.18	.529 .276 .154	.077	1.44 .425 .148 .081
TRICHLOROETHYLENE	XYI.KNF
Applied Kf f luent t run __ Applied El 1 luont t ton
First Second Third	First Second Third
0 X 57 X	68 X 96 X
44.3	10.7	4.45 .13?
19.5 5.77	1.41
40.8	14.2	4.87 1.41
169 5ft.4
1.82 1.03
20.5 /.I
0.61 0.26
29. 7
67.7
16.2
2 5.1'
4.77 ?. I i
9.64 2.H /
ro
a\

-------
2C0
100
C3
P
<
cn
c
12
T
, m
Figure 7.83. Comparison of synthetic (•) and domestic ( 0)
sewage variables in 27-9 m2 units operated
at an area loading rate of 10.2 cm/d.
266

-------
sewage. Figure 7.84 compares the removal of total chemical oxygen demand in
domestic and synthetic wastewater as a function of application of flow rate
in the system. This Indicates that at many of the loading rates the
removals that were observed were similar. Tables 7.22 and 7.23 show a
comparison of the mass removals of various pollutants for the two different
substrates studied. In general, the rates observed for the various para-
meters where the loading rates were comparable were in the same order of
magnitude. This information should be useful in enabling the system to be
defined both in this study and others for the application of typical waste-
water to the NFT system.
The average performance of the two systems at the varying loading conditions
is also summarized in Tables 7.22 and 7.23. This information summarizes the
large amount of data that was developed for the pilot application and as
presented earlier under the various pollutants.
I. DEVELOPMENT OF PREDICTIVE RELATIONSHIPS
Two approaches were examined to predict effluent quality from NFT units
applied to wastewater treatment. First is a correlation of loading rate
relationships to the effluent quality. This approach included an attempt to
correlate plant yield information to removal efficiencies of various pollu-
tants. The earlier figures correlating remov&l rates are useful in estimat-
ing area requirements for treatment systems. The second approach was to
apply simple removal kinetics to the information to define the time required
for varying influent concentrations to achieve a given level of efficiency.
A comparison and summary of the removal rates presented earlier are given in
Table 7.24 as examples from which the size of an NFT system might be
predicted. Some of the limitations of this information include restricted
nutrient uptake and yield due to the dual application of suspended solids
and BOD. At no time was a high quality effluent from an optimized treatment
system fed to healthy, high-yielding plants to test the nutrient removal
capacity.
A kinetic examination of the data was performed using the assumption that
the flow in these units approached plug flow and that the removal of the
pollutants was first order and described by the following equation:
Se = S0 • e-kt	(7.4)
where Se = effluent concentration
S0 = influent concentration
k = removal rate coefficient
t = time of exposure of the reaction or the hydraulic retention time
in the unit
267

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I
Ci:
c:
>-
x
200
c
c;
K
X
O
lOO
influent Effluea
0	5	10	15	20
AREA LOADIMG RATE, cn/d
Figure ?-3U. Comparison of chemical oxygen demand
reductions in domestic and synthetic
sewage a~ various leading rates in
2^.9 ni2 units.

-------
TABLE 1.22. SYSTKM PEKKORMANCK SUMMARY OF PI LOT SCALE SYSTKMS USING SYNTHETIC SEWAGE.
Turner
COD
Soluble COD

local
Phosphorus

Soluble
Phosphorus
Total
Nitrogen

Soluble
Kltroxer

Cadmium

Soluble
Cadmium

Area L.K.
eui/d
toss I..
kg/ha-d
K. Removal
ktf/ha-d X
Hush L.R.
kg/ha-d
Removal
kg/ha-d
X
Mass I..K
kg/ha-d
. Removal
kg/ha-d
X
Mass L.R
k^/ha-d
Removal
kg/ha-d
X
Mass L.R
kg/ha-d
Removal
kg/ha-d
X
Mass L.R.
kg/ha-d
Removal
kfi/ha-d
X
Mass L.R.
kg/hn-d
Removal
kg/ha-d
X
Mass L.R.
kg/ha-d
Remnva1
kg/ha-d
X
10.2
412
244
59
412
245
60
16.9
0.2
1
17.7
1.1
6
38.7
6.7
17
38.8
9.8
25
.112
.092
82



Hi.2
318
150
4 7
382
201
53
10.9
1.2
11
13.5
3.9
29
42.2
12.5
30
38.3
9.0
24
.112
. 102
91



10.2
J//
104
28
392
129
33
16.0
1.2
8
16.2
1.1
7
34.2
Increase
-
39.2
8.6
22
.061
.041
67



10.2
407
257
63
382
234
61
14.3
2.2
16
13.7
1.4
10
36.3
6.5
18
38.7
10.7
28






5.1
213
187
87
213
182
85
7.2
0.7
10
7.2
0.5
6
19.0
4.2
22
19.3
4.6
24
.168
.143
8'j



10.2
2H0
241
86
2/8
244
88
15.1
1.2
8
14.1
0
0
41.9
9.6
23
41.4
8.0
19
.214
.163
70



5.1
133
101
/ 6
128
103
80
7.4
3.8
52
7.2
3.8
53
18.8
6.0
32
18.3
7.3
40
.076
.046
60



20.3
392
265
68
376
250
66
29.3
Increase
-
29.5
Increase
-
71.1
15.6
22
68.5
13.2
19
.305
.122
4(1



10.2
210
191
91
101
87
86
9.9
Increase
-
13.5
3.3
24
34.1
19.5
57
37.0
12.8
35
.193
.132
68



20. J
391.
342
87
391
338
86
27.1
0.2
i
27.7
1.0
4
35.8
Increase
-
38.3
Increase
-
.509
.285
56



6.9
18/
154
82
194
170
87
11.3
0.9
8
10.7
0.3
3
26.5
5.9
22
25.3
5.6
22
.020
0
0
.014
.007
50
10.2
185
137
/4
173
131
76
13.4
2.3
17
12.3
0.9
7
39.7
6.6
17
37.6
6.0
16
.183
.132
72
.173
.142
82
20.3
384
283
81
375
310
83
12.0
0.4
3
12.2
1.2
10
36.2
12.6
35
34.8
12.8
37
.122
.081
67
.122
.102
83
40.6
972
414
43
903
461
51
50.1
Increase
-
45.6
Increase
-
140.8
8.1
6
132.3
2.0
2






6.9
144
46
32
151
101
67
6.2
Increase
--
5.7
Increase
-
13.3
Increase
-
11.3
Increase
-






10.2
460
330
72
390
311
80
16.9
0.1

15.0
Increase
-
57.1
4.8
8
58.6
7.8
13







-------
TABLE 7.23. SYSTEM PfcttyORMANCE OF PILOT SCALE SYSTEMS USING DOMESTIC SEWAGE
Target
BOD
TOC
COD
Soluble COD
Total
Nitrogen

Total
Phoaphorua

Suapeoded Sollda
Volatile
Suapended Solida
Soluble BOD
Area L.K.
ca/d
Has* L.I
kg/ha-d
Braoval
kg/ha-d
Z
Haas L.
kg/ha-d
R. Reaoval
kg/ha-d
Z
Maaa L.l. Reaoval
kg/ha-d kg/ba-d
Z
Haas L.S. Reaoval
kg/ha-d kg/ba-d
Z
Maaa L.t
kg/ha-d
Beaoval
kg/ba-d
Z
Maaa L.S
kg/ha-d
. Reaoval
kg/ha-d
Z
Maaa L.t.
kg/ba-d
Reaoval
kg/ha-d Z
Maaa L.R.
kg/ha-d
Reaoval
kg/ha-d
Z
Maaa L.R. Reaoval
kg/ha-d kg/ha-d
Z
5.1
4.0
10.2
172
129
/5
132
97
73










117
96 82
54
41
76


10.2
109
67
61
75






44.5
2.8
6
7.22
0.38
5
73
45 62
65
41
63


10.2
103
69
67
84
47
56




41.2
6.5
16
6.31
1.22
19
68
40 59
46
33
72


5.1
62
44
71
42
26
62




20.2
5.7
28
3.77
1.21
32
47
36 77
35
27
77


; 20.3










67.9
12.7
19
12.96
1.77
M
106
153 74
180
145
83


5.1
58
48
83
48
35
73




17.5
1.1
18
3.05
0.71
23
46
31 67
29
20
69


10.2
101
80
79
64
43
51




27.3
4.6
17
4.84
0.39
8
no
78 71
108
95
88


20.3
133
118
89













175
164 94
174
167
96


6.9
67
61
91
49
35
72
133 99
74
94 62
66
13.0
3.3
25
2.97
0.67
23
20
17 85
24
22
92
44 38
86
10.2
182
134
74
101
59
58
372 190
51
272 113
42
36.4
11.1
30
7.62
1.67
22
57
48 04
50
42
84
120 78
65
20.3
272
166
61
100
51
51
556 247
44
395 144
36
44.4
6.9
16
12.09
1.12
9
98
66 67
87
56
64
150 74
47

-------
TABLE 7.24. COMPARISON OF MEAN MASS REMOVALS FROM SYNTHETIC AND DOMESTIC SEWAGE
IN PILOT SCALE SYSTEMS. (kg/m2-d)
Domestic Sewage
t

Synthetic
: Sewage

Average Flow (A/d)
1563
3804
5773
1913
2834
5668
11336
Target Loading (cm/d)
7
10
20
7
10
20
40
Total BOD
.0062
.0125
.0131
—
—
—
—
Soluble BOD
.0042
.0078
.0064
—
—
—
—
Total COD
.0105
.0181
.0197
.0134
.0137
.0295
.0496
Soluble COD
.0065
.0104
.0104
.0153
.0138
.0315
.0522
Total TOC
.0038
.0056
.0048
—
—
—
—
Total SS
.0025
.0047
.0064
+
+
0
+
Volatile SS
.0022
.0040
.0054
1.4 x 10-I+
+
0
+
Total N
5.3 x lO"1*
8.3 x 10-1*
3.9 x lp-1*
6 x 10~5
7 x 10~5
1.4 x 10"1*
+
Total P
9 x 10~5
1.0 x lO-*4
2 x 10-5
.0001
.0002
8 x 10-5
+
CD
—
—
—
i
o
X
1 x 10~5
1 x 10-5
2 x 10-5
- = Not determined
+ = Increase in concentration through system

-------
By plotting the removal efficiency versus the hydraulic retention time in
the system it is possible to obtain an empirical determination of k, the
substrate removal coefficient. If the data follow a straight line on a
semi-log plot, it also confirms that the reaction rate is driven by the
substrate concentration in the reactor and that it is a first order rela-
tionship. Departures or scatter of the data reflect the fact that the
system is being tested in a substrate-limited zone, that the reaction is not
first order, or that the system is a significant departure from a plug flow
and that mixing and short-circuiting occurred.
Example plots of rate determinations for BOD, suspended and volatile solids,
nitrogen, and phosphorus for domestic sewage are shown in Figures 7.85
through 7.89. Similar plots for COD, nitrogen and phosphorus removal from
synthetic sewage are shown in Figures 7.90 through 9.92. The substrate
removal rate coefficient as determined from this information is summarized
in Table 7.25. This information, although quite variable, tends to indicate
that the removals are first order for most of these systems. Because of the
variability of the data, this approach has a limited value in the design of
this system. The application of this information to sizing the units will
be presented in the next section.
TABLE 7.25. SUMMARY OF SUBSTRATE REMOVAL COEFFICIENTS
AS DETERMINED FROM ASSUMED FIRST ORDER
KINETIC ESTIMATES FOR CANARY GRASS SYSTEMS
Sewage Type
Variable
k
Domestic
BOD
-0.0015

TSS
-0.0096

VSS
-0.010

N
-0.0016

P
-0.0016
Synthetic
COD
-0.0038

N
-0.0001

P
-0.0008
272

-------
1 • \J
0.9
0.8
-


k = -.0015

0.7
—




0.6
—

•


0.5
—



•
o. u
—




0.3
—




0.2
—


•

0.1

1 1 1
1 1
1 1 1 1
1
0 10 20 30 1<0 50 60 70 80 90 100 110
IIYDRAIILLC: RETENTION TIME, mill.
Figure 7.85. liat.e d<;UM-;ni nation plot for BOD removal L t'rom dome:; I i c
b.y a canary gratis system.

-------
k = -0.00960
)i
1
80
120
60
100
20
1IYDRALJ],IC RETENTION TIME, min.
I1'i. guro 7.86. Rate dotormi na I, loii plot for suspended solids removal from
domestic newage by a canary grasn system.

-------
0
9
8
-0.010
.6

Ji
3
2
"I ?.()
} 00
HYDRAULIC RETENTION TIME, min
Figure	Hate detei'ininatiori plot for voJub Lie suspended .solid:;
removal. Croin domestic sewage by a canary p,raan :;y:itoin.

-------
.0
0.9
0.8
0.Y
0.6
O.H
o
0.
0. i
00
120
100
60
20
HYDRAULIC RETENTION TIM]''., mi n .
Figure Y-00. Rate detcrminat.ion plot for total nitrogen removal from
domestic sewapc by a canary grass system.

-------
0.8	-
0 • 7	-
0.6	-
o.^	-
0. )|	-
o
t n
0.1 	I	I	I	l	I	L_
0	1*0	6o	80	i00	120
HYDRAUL,fC RLITKNT10N '[MMK, min.
Figure 7• 89 • Hate dotermi nat.i on plot for total phosphorus removal, from domestic
sewage b.y a canary grtisr; system.

-------
9
8
Y
18.6 m2
6
o
j.
160
200
80
ho
120
HYDRAULIC HKTENTTON T'L'MF!, rnin.
Figure '(.90. Kate determinat. ion plot. I'oc COD rcmova] from synthetic sewage by
canary gra:;^ unit'.; in different size sy ytoma.

-------

L • > '
0.9
zrv	
A "AO
		~


0.8
-




0.Y
-




0.6
-




0.5
-



0
(O
O.'l
-

e
9.3 ni2 systems






0)
cn
0.3
-

~
18.6 m2 systems




A
27.9 "i2 systems

0.2
—







k
= -.0007:;

0.1

1
1 1
1
o	UO	Bo	120	160	200
UYDHAULLC RETENTION TIMK, min.
Figure T-9L.
Rate determination plot for' phosphoi'us removal
from .synthetic sewa,'.;e by canary {jriiaa units in
di ITerent size unit:;.

-------
1.0
0.9

11 A *
~

A
0.8
0.7
5
0 & 0
0


~
0.6
-

~


o.b
-




o.U
-




0.3
-


0
9.3 1,12 uyi-itenu;
0.2



~
A
|8.6 m? :;y:;tems
27.9 m2 :sy:;l«rnri
0.1

1
1 1

1
0	ho	Bo	120	.160	200
HYDRAU1.T'": RUTKNTJON '['IMK, mi 11.
!¦'j fr,urc 7-92.
Ral.e (ict'.erinj nut, i ori for tola"! ni t.rogen removal
from ;;yril'.he'...ie uowuf,c in canary (-',raas unit::-,
or different area:;.

-------
CHAPTER VIII
DISCUSSION
A. GENERAL CONSIDERATIONS
The general goal of this large study was to provide data to support a
feasibility analysis for the use of plants for wastewater purification. The
experimental program was designed to test the NFT under conditions that
would make it a viable alternative to conventional technologies. Units up
to 36 meters long were tested at flow rates up to 11,000 liters (3,000 gpd)
per day. Although a detailed costing analysis is beyond the scope of this
study, it appears that the NFT will achieve the goal of being competitive
with most conventional technologies; that is, secondary treatment efficiency
or better can be achieved with the NFT system for a similar or lower cost.
The testing program was conducted in such a way that a given loading rate
was tested to the point of achieving relatively constant results, and then
an Increased loading rate was imposed on the system until treatment failure
or plant death occurred. This approach resulted in the testing of plant
systems that were relatively immature under highly adverse conditions.
Thus, the results of this study should be considered conservative. Since
the results also reflect plant activity in a cold climate, the capability of
the system should improve with higher incident solar radiation and tempera-
ture. The disadvantages and the advantages of the NFT system over conven-
tional treatment systems are listed in Table 8.1. In general, this study
provides the Information that enables all plant species to be considered as
potential candidates for a wastewater treatment system. The weeds and other
marginally valued plants can all be useful, but the NFT enables considera-
tion of trees, shrubs, food plants and pharmaceutical producing plants as
potential candidates. Existing agricultural practices for propagation,
growth, harvesting and pest management can be applied to the NFT system.
The operation of units In northern New York and New Hampshire indicates that
a cold climate is not a severe limitation and that the system would operate
anywhere in the contiguous United States. Another possible advantage of the
NFT system is the value of the potential by-products when ornamental, food
or biochemical products are considered. As will be Indicated under the cost
anaylsls, the covered system and the size of the NFT that is required to
provide effluent quality appears to be competitive with the conventional
treatment systems. Finally, a significant advantage is the psychological
Impact of a treatment system which produces a beautiful and potentially
valuable by-product from a wasted resource. The Impression gained from a
bed of roses in blossom when visitors enter the sewage plant provides a
significant asset for the treatment system.
281

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TABLE 8.1. ADVANTAGES AND DISADVANTAGES OF THE NFT SYSTEM WHEN USED FOR
WASTEWATER TREATMENT
ADVANTAGES
1.	All plants are potential candidates
2.	Existing agricultural techniques applicable — propagation,
growth, harvesting, pest control, etc.
3.	Not limited by climate — applies anywhere In U.S.
4.	Potentially valuable by-products
5.	Covered system cost-effective compared to conventional
6.	Psychological Impact of treatment system appearance
DISADVANTAGES
1. Limited by all factors that apply to biological treatment
-	toxic response
-	seasonal affects
The major disadvantages that limit the application of the NFT system are
essentially those that apply to all biological systems. The recovery of the
system from toxins will be slow and costly. In northern climates the
seasonal effects are significant and will affect effluent quality.
Due to the Interaction of the large number of variables in the NFT, It was
difficult to define all of the mechanisms affecting pollutants in domestic
sewage. Parameters that are important will be discussed below. However,
several general observations can be made, regardless of the climate and
location of the NFT system. Effluent total COD concentrations of less than
100 mg/£, BOD5 values less than 30 mg/£ and suspended solids less than 10
mg/£ were often achieved at loading rates of 20 cm/day with NFT unit lengths
of 24 and 36 meters. Effluents of less than 10 mg/£ of suspended solids
occurred under loading rates of 30 cm/day with the longer test cells under
virtually all conditions.
In general, the plant productivity and nutrient removal aspects of the NFT
were not remarkable. This is attributed to the low temperature and Incident
light conditions in combination with the high loading rates of domestic
sewage pollutants that were examined for most of the test period. The
original system was proposed to be useful for manipulating the nitrogen
cycle via nitrification-denitrification. However, conditions never enabled
nitrification to occur to any significant degree.
B. NFT CHARACTERISTICS AND POLLUTANT REMOVAL MECHANISMS
B.l. Settling, Filtration, and Suspended Solids Control
The accumulation of solids in the root mass leads to the possibility of
pollutant manipulation in the NFT system. Background suspended solids lost
from a biological processing NFT can be estimated from the synthetic sewage
studies since the influent solids were always low. At lower loading
282

-------
conditions of 5 to 20 cm/day of a synthetic soluble wastewater, effluent
suspended solids resulting from microbial growth were always less than 5 to
7 mg/&, and these usually occurred in a coagulated, flocculated form so that
the effluent clarity was quite high; in other words, the turbidity was low.
At the higher loading rates, effluent suspended solids never exceeded
10 mg/£.
A consideration of the loading rates and the average flow-through velocities
indicates that most suspended solids should be easily removed at relatively
high loading rates in the NFT system. What is undefined are the mechanisms
that encourage small, colloidlal-sized bacterial solids to coagulate and
form larger particles that could be removed from the system by sedimentation
and filtration processes. It was anticipated in the hypothesized system
that the solids would attach and concentrate within the root mass. These
solids would then gradually induce additional root production so that the
root depth would increase and thus provide a mechanism for suspended solids
stabilization and control. In most cases, this did not occur. The sus-
pended solids were effectively blocoagulated in the unit, but they remained
relatively light and flocculant, and moved freely in the system. The
results of this interaction suggest that the controlled hydraulic loading
and unloading of these units could lead to separation and accumulation of
suspended solids In the reactor. At a specified time these solids could
then be flushed from the system in a relatively concentrated form. This
alternative was tested in the latter phases of the study and was found to be
effective for the treatment of raw sewage. Intermittent loadings of 30
cm/day were found to result in complete removal of settleable solids, 89
percent removal of suspended solids, and 77 percent removal of COD. These
extraordinarily high efficiencies were achieved in short-term experiments
and should not be taken as conclusive results; but they do represent an
encouraging optimization of the system.
The solids control capability of the NFT system and manipulation represents
one of the important advantages of the NFT system. In short-term experi-
ments attempting to document the capability for accumulating solids and the
rate of accumulation of solids £t was found that blomass entrapped In the
root zone often equalled 1 kg/m and that It accumulated at rates of between
2 and 548 gm/m /day (See Tables 7.4 and 7.5). This large amount of micro-
bial solids represents an interesting reservoir for manipulation of various
pollutant removal mechanisms. This large organic reservoir combined with
low redox potential, Is one of the reasons for the efficient cadmium
removals, for example.
The accumulation of solids in the NFT system Is predictable based on the
biodegradable organlcs and the suspended solids in the system. For example,
if It is assumed that 80% of the suspended solids and BOD are removed from a
domestic sewage, then 200 mg/I of suspended solids and approximately
160 mg/£ of BOD would be removed based on a medium strength domestic
sewage. The minimum microbial yield under these conditions is approximately
32 mg/4 of refractory microbial suspended solids and about 50 mg/A of
remaining undegraded suspended solids. Thus the wastewater can be consid-
ered to add approximately 80 mg/£ of solids that could be entrapped within
the root zone if removed from the wastewater. At a loading rate of
283

-------
10 cm/day, it would be expected that each square meter would accumulate
approximately 8 gm of suspended solids per day, and this would increase
proportionally with the hydraulic loading rate. Since these organics are
relatively concentrated, this adds approximately 0.013 cm/day of organic
matter. Based on the concentration that accumulates in the system and the
total mass in the system, it can be assumed that the solids retention time
in the NFT root zone is high, being greater than 125 days in a mature NFT
system. This long solids retention time of coagulated solids in the root
zone represents a large population of microorganisms that could be used to
manipulate many of the pollutant cycles. The capability of this microbial
community was limited in this study because of the high loadings that were
tested under most conditions and the resulting low dissolved oxygen levels.
The efficient biocoagulation and separation of organics from settled and raw
sewage indicate an important role for plants in achieving solids separa-
tion. This may be an alternative to clarification processes. Clearly, the
accumulation and the use of this large amount of organic matter must be
limited to application of plants that can withstand low dissolved oxygen
levels and redox potentials. Swamp plants such as bulrushes and cattails
are excellent candidates for this particular part of the system.
B.2. Microbial Metabolism and BOD and COD Removal
Microbial metabolism of the BOD must be the main organic removal mechanism.
This in turn is controlled by the oxygen aeration rates. Organic removals
were less impressive than suspended solids removals. Typical effluents of
less than 10 mg/& BOD occurred when treating sewage in the 36 meter long
units at loading rates of 7 cm/day. At the higher loading rate of 20 cm/day
the effluent BOD Increased to 40 mg/£. At this higher loading rate the dis-
solved oxygen levels were often close to zero throughout most of the length
of the system. Even at the lower loading rates dissolved oxygen was usually
no greater than 2 or 3 mg/A, indicating significant microbial activity.
The high efficiency of syspended solids control and lower capability for BOD
control indicates several possible alternatives for configurations of the
NFT system. For example, high rate preliminary treatment NFT units could be
used to efficiently separate solids and achieve perhaps as much as 50 per-
cent BOD removal. Aeration by a low energy-consuming technique such as
cascades could be inserted between this roughing NFT system and the
following organic removal step. High rate processes that efficiently remove
organics might be considered for installation after this unit prior to the
addition of a polishing and nutrient removal NFT section.
Although it was difficult to quantify the relationship of the plant growth
to the loading condition, the dissolved oxygen and the organic loading rates
were clearly a major controlling factor for plants that do not have physio-
logical adaptations for growing in anaerobic environments. For example,
long-term tests Indicated that influent organic loadings with 12 meter long
units at 20 cm per day exceeded the tolerance of the reed canary grass.
Long term operation at 20 cm/d at BOD values of 200 mg/A or greater would
kill healthy reed canary grass in several weeks. Also, several days'
284

-------
submergence of a root mat, such as that of napler grass, would result In
death of the plant.
The low turbidities that we achieved at relatively high loading rates were a
direct result of the blocoagulatlon that occurred in the system. An example
of the clarity of the effluents can be seen In Figure 8.1 showing the accu-
mulated solids in the bottom of an overflow trough and high clarity water
being discharged from the unit. Efficient separation of solids throughout
the testing indicates that alternate flushing and sedimentation may be
necessary and useful to obtain complete control over solids in the system.
High turbidity effluents should be expected during flushing cycles.
Finally, it should be emphasized that roughing NFT systems operating at low
or zero dissolved oxygen concentrations should be expected to generate
malodors. Since in the northern climates a greenhouse is required, it
should be possible to separate these odors and to strip them before dis-
charging the air from the greenhouse. Odors in subsequent portions of the
system were insignificant throughout most of the study.
Efforts were made to estimate the Impact of the NFT on reaeratlon character-
istics, and indirectly to determine the role of the plants in organic oxida-
tion. Due to the complexity of the factors affecting reaeratlon, no conclu-
sive insights were developed. Attention to this topic in future studies
will assist in optimizing alternative NFT configurations for organic
removals and nitrogen cycle manipulations.
B.3. Nutrient Removals
The use of plants for nutrient control is an old technology, especially with
the application of microscopic algae in wastewater ponds. This use of
plants for nutrient removal, however, raises several problems. Large land
areas are usually required. Figure 8.2 illustrates the relationship between
nitrogen content and yield of plants and the area required to treat domestic
sewage based on nitrogen removal. Exceptionally high plant yields are
required for nitrogen control before the land area requirements are small
enough to represent practical treatment areas, especially where greenhouse
covers may be necessary. Literature values Indicate that many of the plants
that were studied, such as cattails, have the capacity to yield up to 80
t/ha-yr when the root mass is included. With the higher nitrogen content,
this plant yield is such that as few as 15 ha of land would be required for
nitrogen removal from sewage produced by 10,000 people. However, this
represents a large surface area, and one that would be costly to enclose.
It is likely that lower efficiencies for nutrient removal would be achieved
with the NFT system. Another possible limitation for nitrogen and phospho-
rus removal with plant systems is the relationship between the nitrogen and
phosphorus in sewage and the ratio in plant matter. The concentration
ratios of nitrogen to phosphorus can be as low as 2:1 In settled or
secondary treated sewage, whereas plants often have a nitrogen:phosphorus
ratio of 10:1. Certain plants also have other requirements that raise
special problems when applied to domestic sewage. For example, many plants
require large quantities of potassium and iron, two elements found at low
concentrations in domestic sewage.
285

-------
Figure 8.1. Photograph of the effluent, from an NFT unit
treating primary settled sewage showing the
the large coagulated, particles settled in
the overflow trough.
286

-------
100
o
H
o
o
in
10
P-t
tn
w
%
LH
O
ro
m
E<
U
ui
CO
N
m
W
AlU'.AiJ
WLTI1
CONVENT.LONA
PLANT YIELDS

AKEAU "
ADVANCED
AC k.i cut/run i
TECHNIQUE:
iTlCAL
1'OTENITAL YIELD£>
KENI10USE
i LELDS
0 10	:'0	UO 60 80 LOO	L'OO	1+00
PLAN!' YIELD, METRIC TONNES PER HECTARE
h'igure 8.P. h'e I at, iour.h Lp ol' pi ant yield to land area required for
complete nitrogen removal from domestic sewage for
crops with two different levels of nitrogen. Tt. jr.
assumed that the sewage has HO ing/8. of total nitrogen,
that it is all available to the plants at the initial
high rate of uptake, and that the per capita flow rate
is O.h inJ / 
-------
In tropical areas nutrient removal with the NFT may be more promising. How-
ever, in the colder climates the attractiveness of nutrient control is
limited. It is likely that the nutrient film can be used to produce valua-
ble plants and a marginal nutrient removal efficiency. Where additional
nutrient removal is required, a combination of other higher rate biological
and chemical processes may be required. Finally, it should be noted that in
areas where seasonal discharge limitations are acceptable, biological
processes such as the NFT may be useful. Significant nutrient removals can
be achieved in the summer periods where temperatures are higher and light
intensity greater in temperate climates, but during the colder times of the
year when low radiation occurs, little nutrient removal may result.
C.	TOXIC SUBSTANCES
Data obtained on heavy metals and toxic organics indicate that the NFT can
effectively remove toxic materials. Zinc and cadmium concentrations of 0.1
to 0.3 mg/& were efficiently removed from the wastewater down to a level of
0.06 mg/i or less, thus meeting effluent standards for irrigation and higher
quality uses. These metals were concentrated in the volatile solids entrap-
ped by the roots. The efficiency is probably related to microbial activity
and the low redox potential. However, a significant portion of the cadmium
that was removed from the wastewater was transferred into the foliage of the
reed canary grass. Any use of the plant material mist take into considera-
tion the concentrations of the toxic metals in the plant matter.
The NFT unit also appeared to be efficient in removing volatile and non-
volatile organics. As in overland flow land treatment, the large surface
area of the NFT enhances the chances of losing volatile organics such as
chloroform (Jenkins, 1981). Non-volatile toxic organics such as PCB's
appear to be absorbed in the root zone. This stripping of toxic organics
needs to be defined in future activities so that the fate of these materials
can be predicted.
Although it was not anticipated that the indicator bacterial organisms'
reduction would be significant enough to result in the elimination of a
disinfection process, significant removals could occur if the NFT system
were optimized. The lower loading rate condition usually resulted in large
decreases in fecal coliforms.
D.	TDS MANIPULATION
The introduction of the NFT system provides the design engineer with the use
of the entire plant kingdom as treatment mechanisms. There is potential for
dissolved solids control with plants under certain circumstances when using
the NFT system. This possibility, however, may not be realized since the
uptake of dissolved salts is closely related to plant yield and evapo-
transpiration. The relationship between plant yield and dissolved solids
control is illustrated in Figure 8.3 where three different loadings are
compared to varying plant yields with varying ash content. With most
288

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100
90
80
Y0
6o
l;0
no
30
:>o
]0
T.ine
Hydraulic
Loading
Rate
cm/d
1
L0
10
Plant
Ash
Content
% TW
10
10
30
30 3 '3 1|0 1*5
I'TiANT Y1RT.D, p;m/m"-d
60
P'Lfrure ft. 3-
Calculated maximum total diusolved suits removal. efficiency with plants
in the NFT at varying yields. The influent total dissolved solid:.;
concent,ration is assumed to be [300 tug/&. ('IT is assumed to be zero.

-------
plants that have relatively low ash content, efficient removal of dissolved
solids could occur, but only at extremely high yields. Note that it is
assumed that ET is zero in Figure 8.3, a simplifying assumption that does
not occur in plant metabolism.
E.	TEMPERATURE EFFECTS
The effect of temperature on the NFT treatment system has both negative and
positive aspects. Studies with the NFT indicate that plants that maintain
their roots in warmer solutions can withstand relatively cold temperatures
around the vegetative portions and still grow at significant rates. Root
temperatures of 10°C will be expected in all but the most northern cli-
mates. Temperatures as low as 7°C occurred in New Hampshire. This did not
kill the plants, but it did limit their productivity. It is anticipated
that the heat provided from the sewage would minimize the amount of heating
for the greenhouse that would be required in northern climates in order to
maintain acceptable air temperatures. The combined use of solar greenhouse
techniques with large water flows with minimum temperatures of 10°C could
eliminate the need for additional heating with marginal quality plants.
The negative aspects of the temperature effects relates to the solar heat
absorption that occurs in the greenhouse and the possibilities of thermal
pollution effects. The large surface area required to treat the sewage
provides an ideal opportunity to capture solar heat in the water. When
feeding colder temperature sewage at 7°C, temperature rises greater than 10°
were observed in January and February in New Hampshire. In the summertime
the temperature increase appeared to be less, although this may have
reflected the Impact of shading by the more mature plants that were tested
in most summer operations. The relationships of temperature and greenhouse
requirements should be contrasted to the storage requirements with land
application systems. In the northern U.S. climates, more than 10 ha of
wastewater winter storage would be required for a population of 10,000
people.
Another advantage of a greenhouse cover Is that it enables complete control
of pests and pest management techniques. Biological controls (such as with
the ladybeetles tried here) and chemical controls are easily contained.
F.	PROCESS SENSITIVITY AND DESIGN
The relationship between the major loading rate parameters and the
efficiency for various pollutant removals were examined to provide a predic-
tive relationship between wastewater quality, NFT area, and effluent
quality. Several loading rate parameters affect the removal mechanisms.
The hydraulic and weir loading rates to the system dictate the efficiency of
the suspended solids and BOD removals, and also indirectly determine the
hydraulic retention time, which influences other pollutant removal mechan-
isms. The Results section outlined techniques to relate the loading rate to
the hydraulic retention time under a specific set of conditions. Unfortun-
ately, due to the large number of variables (plant species, reactor length,
depth of liquid, temperature, incident radiation, and pollutant concentra-
tion) no consistent theory of kinetics of removal for each pollutant was
290

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defined. However, empirical removal rates at given loadings can be used as
a first approximation of the size of the system required to achieve a given
effluent. The dissolved synthetic sewage used at Cornell generated by the
Cayuga Heights community in New York, and that of the Hanover, New
Hampshire, community were similar within certain limits, and can be used for
empirical estimates of process sizing and efficiencies.
As indicated in the Results Chapter, loading rates up to 20 cm/day resulted
in effluent qualities exceeding secondary standards in most cases. The
removal rates reported in Tables 7.22 and 7.23 express the range of removals
achieved at the varying loading conditions. During lower loading rates the
systems worked more efficiently, while at higher loadings the systems were
receiving more pollutants and were operating less efficiently for many of
the pollutants. Given a loading rate, the area required for treatment can
be calculated from the removal rate when it is expressed as kilograms
removed/ha-day. For example, if the COD removal rate of 900 kg/ha-day cou^.d
be achieved continuously in an NFT system, a design loading rate of 4500 m
applied/ha-day would result, or a hydraulic depth of loading of approxi-
mately 45 cm/day would be acceptable (assuming that the COD concentration is
0.2 kg/m ). The hydraulic retention time that is required to produce this
effluent is not known specifically; however, the volume of the system with a
given application rate defines the hydraulic retention time. In this case
the hydraulic retention time in a 36 meter long unit would be approximately
95 minutes if the system responded similar to the test given in Figure
7.45. Deeper or finer root masses with varying void spaces and accumulation
of different levels of biomass will change the system's volume and thus the
hydraulic retention time. The relationship of hydraulic retention time to
efficiency is an area where much additional work needs to be conducted
before rational design of the NFT process is possible. Example loading
rates and removal rates are listed in Table 7.23 for removal of nitrogen,
phosphorus, and cadmium as obtained from the empirical loading rates that
were achieved in the system. Bench scale kinetic work also provided the
removal rate values which can be contrasted to those obtained under steady
state with the larger system. Maximum BOD removal of 50 gm/m -day were
measured with small reed canary grass units. This indicates that an
application of 25 cm/day would result in an acceptable secondary effluent.
G. PLANT ENVIRONMENT FACTORS
A wide range of plants were tested to determine their viability under
varying loading conditions and adverse environmental conditions. Although a
large amount of information remains to be developed to define the specific
conditions that will lead to maximum yields, considerations for plant
production, harvesting, and management were defined in this study. The main
candidate species, reed canary grass, was used throughout the study and was
found to be viable throughout the four seasons in the cold climate of the
United States. However, the most sensitive parameter appears to be related
to the organic loading rates on the root system and, indirectly, to the
dissolved oxygen concentration. At a 20 cm/day loading of medium strength
sewage, the reed canary grass will eventually die. The reason for this
291

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death is likely the lack of dissolved oxygen reaching the root system to
provide metabolic requirements. In actual systems it is recommended that
the organic loading rate be limited when plants that require an aerobic or
facultative root system are utilized. This can be accomplished in a sewage
treatment system by utilizing a roughing treatment unit upstream from plants
that are more sensitive. Plants that feed oxygen to the roots through their
structure, such as the bulrushes, may be viable for encouraging the bioco-
agulatlon and stabilization of sludges. The extent of aeration caused by
such plants was not measured in this study.
It is essential that the photoperiod and temperature interactions be
carefully evaluated in choosing a plant species. One candidate species
tested, phragmites, grew rapidly during the warmer parts of the year; but
when the photoperiod declined to a certain duration, phragmites became
dormant; and growth ceased until the photoperiod reached the minimum
length. In New York the dormant period occurs between mid-December through
the first of March. Phragmites' dormancy and reaction to the light period
was in contrast to the reed canary grass, which remained green and healthy
throughout several years of testing in the NFT unit. Surprisingly, the
tropical napier grass also remained green and growing throughout the entire
year.
It should be emphasized that the combination of organic loadings and low
oxygen tension in the root zone provide an inhibitory environment for many
plants, and the presence of large quantities of bacteria and organic matter
provides an excellent environment for the growth of pests. As indicated in
the Results section, numerous crises were experienced where a pest would
grow to a point that would threaten the entire plant system. In all cases,
the organism was controlled by a plant management scheme, by manipulation of
the water flow, or by the addition of pest controls. It is essential in
considering this type of system that the expertise gained in greenhouse and
plant management be used since all of the problems that occurred have
essentially been solved by plant scientists. A full scale NFT system would
require the services of a horticulturalist or other plant specialist.
In general, the more valuable plants would be exposed to the wastewater at a
point in the system where the stress on the plant would be minimzed. In
other words, the roses that were produced in this system grew well when the
sewage had received treatment to a level that removed most of the biodegra-
dable organics and suspended solids. This suggests that the latter part of
the NFT system where the wastewater is relatively clean could be used for
production and testing of a number of different species for community or
commercial utilization.
H. OPTIMIZED FACILITY CONSIDERATIONS
The pollutants which must be removed from sewage range from the large solids
to toxic trace organics. The fundamental mechanisms that control their
removal may not be compatible with one another. For example, a continuously
flowing system where the biochemical materials are exposed to the bacteria
is the most favorable environment for rapid removal. However, suspended
solids management may be achieved under quiescent conditions much more
292

-------
readily than in a continuously operated film unit. It is also likely that
the solids accumulation within the reactor will be useful for further
pollutant reduction if they can be accumulated in the root zone and
intimately mixed with the wastewater over a short period of time and
efficiently removed.
Consideration of the pollutant removal mechanisms and the NFT requirements
in terms of oxygen in the root zone and photoperiod led to an optimized
alternative that was tested in the latter phase of this study at the Cayuga
Heights plant. For maximum accumulation of solids in the anaerobic root
zone of roughing plants such as cattails and bulrushes, a rapid addition of
wastewater to the system followed by quiescent draw off into subsequent
units used to polish the remaining BOD and suspended solids was tested. The
preliminary results from this testing show that at an overall addition of
30 cm of raw sewage per day, 100 percent of the settleable solids was
removed and contained in large particles that were biocoagulated; 75 to 80
percent of total COD was removed; 90 percent of the suspended solids was
removed; and, surprisingly, 60 percent of the total nitrogen was removed
from the wastewater. These results lead to the consideration of an excep-
tionally small system for roughing treatment followed by a polishing
secondary system that would have water of a high enough quality that many
alternate species could be produced without dissolved oxygen and solids
inhibition in the root zone. These data suggest that the roughing treatment
unit could achieve better than primary treatment with less than 0.5 ha for
sewage flow from 10,000 people (1 MGD). By removing the suspended solids
and the BOD rapidly in this system, further treatment and some additional
nutrient removal could be achieved with several hectares of NFT following
this roughing batch mode type of treatment. This will be the type of design
discussed in the following chapter.
I. PLANT CULTURING AND PROPAGATION
The wide range of testing with weeds and valuable commercial plants con-
ducted in this study indicates that nearly all plants can be considered
candidates for use in the NFT system. By using culturing techniques such as
propagation with cuttings, it is relatively easy to produce large plant
stands. In cases where seeds are available with plants such as cattails, it
is also possible to rapidly produce large stands of these plants.
The major limitations for the plant culturing aspects of the system relate
to the practical consideration for Installation of the system. If plant
culturing takes place after construction it would be necessary to begin
plant production in the spring when the sources of plants are available as
young plants or as seeds. Winter start-up would be a difficult task. How-
ever, there is also the possibility that while the facility is under con-
struction large quantities of plants could be propagated by growers, or
temporary facilities could be constructed to produce large quantities of the
material. For example, temporary NFT propagation units could be established
during warmer weather by spreading plastic on top of the ground and allowing
water to be sprayed as a fine mist over such a surface. This would be one
alternative to providing plant systems that would be available at the finish
of construction.
293

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It should also be noted that the latter sections of the NFT unit can be used
as culturlng and propagation sections for other portions of the facility.
It 9hould be anticipated that pests have the capability of eliminating por-
tions of the system at any time, and it would be wise to have replacement
materials available so that portions could be removed and replaced with new
plant matter. Another option for the latter portion would be to use it as a
nursery area for production of valuable plants for either commercial sales,
or for use in the municipality.
294

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CHAPTER IX
NFT TOTAL SYSTEM FEASIBILITY CONSIDERATIONS
The following total system requirements and some economic estimates provide
a perspective on the potential of this technology. Due to the promising
results obtained from the large size and the duration of these tests, It Is
hoped that this information will lead rapidly to innovative testing of the
NFT process in larger pilot operations and possibly in full scale opera-
tion. The following analysis is divided into two areas. First is a con-
sideration of an alternate secondary wastewater treatment system, and
second, the application of the NFT as the main unit process in tertiary
treatment or water reclamation. Both examples are based on a system large
enough to treat wastes from a community of 10,000 people (wastewater flow of
about 3800 m /d or 1 MGD).
A. THE NFT AS A SECONDARY TREATMENT SYSTEM
A secondary NFT treatment system that would produce effluent quality with
less than 30 mg/£ of BOD and suspended solids throughout the year, even in a
northern climate, was developed using the conservative data of this study
and is illustrated in Figure 9.1. This system is composed of a two-phase
NFT treatment. The first phase is an intermittently loaded roughing NFT
designed to remove all of the settleable solids and a significant portion of
suspended solids and BOD. It has a covered greenhouse area of about one
hectare per 10,000 people served. It is followed In sequence by a polishing
treatment system which has a greenhouse area of approximately two hectares.
The loading rate on the roughing system is approximately 50 cm/day, and the
polishing area receives a flow equivalent to 25 cm/day. The overall loading
rate on the entire treatment system is equivalent to 17 cm/day.
Potential plant species for the system include a variety of the submerged
aquatics such as bulrushes, cattails, and sedges for the roughing treatment
system. The polishing unit would be composed of rapidly growing plants with
large root systems such as the napier grass and the reed canary grass.
Approximately one-third to one-half of the latter unit could be used for the
production of useful plant materials—ornamentals, trees, and shrubs.
It Is beyond the scope of this study to perform a detailed design and cost-
ing of the system. However, some general comments regarding the economics
of the NFT can be made. The unit shown in Figure 9.1 would be built with a
low-cost solar greenhouse type of construction. The greenhouse has a
capital investment of approximately $54/m ($5/ft ). Thus the total capital
investment for the entire treatment system, minus the sludge handling, would
be approximately $1.6 million for a sewage flow from 10,000 people. This
295

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Preliminary
Treatment
Influent
Waste Solids
Zffluent Air
Scrubbed for
Odor Removal
Roughing NtT l.G ha)
to
¦Aeration
EFT for
"Treatment and
utrier.t Conversion
2 ha
\Arsa Available for
commercial plant production
approximately 1 ha)
» Useful Plant
Material
5rr.amer.t al, cheir.ical
or animal feed)
Disinfection
Figure
9.1.
Schematic of NFT treatment facility capable of treating
domestic sewage from 10,000 people.

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contrasts to an average capital investment for conventional technology on
the order of $2 million for a total treatment facility for the same flow.
Energy requirements for this facility also appear to be low, since no aera-
tion and only low head pumping would be required.
Although it is difficult to compare systems, it is interesting to contrast
the area requirements, energy, and economic benefits of a conventional
treatment system, a water hyacinth aquatic treatment system, and an NFT. An
attempt to summarize these comparisons for a flow from a 10,000-person
community is shown in Table 9.1. When applied as a secondary treatment
process, the energy produced from the biomass grown in an NFT process is
insignificant unless anaerobic digestion is used for treatment of the
sludge. In contrast, the water hyacinth has an energy production value
equal to approximately $0.08 per 3785 liters (1,000 gallons). However, this
is a misleading value since the cost of handling the added wet material in
the form of the water hyacinth is often neglected. As shown in the table,
the estimated sludge produced by a water hyacinth facility is more than four
times that produced in either the conventional faciltiy or the NFT
facility. If this is considered, the sludge disposal costs in the water
hyacinth system may eliminate all economic benefits from the energy produced
in that system. In contrast, the potential value from the use of a small
portion of the latter part of the NFT system can be equal to $0.40 to $0.80
per 3785 liters (1,000 gallons) treated. This by-product value assumes that
useful and economically viable plants are produced from only one-sixth of
the area of the NFT unit. If more area could be used to produce
commercially viable plant matter, the NFT would clearly be more attractive.
TABLE 9.1. SUMMARY COMPARISON OF TWO SECONDARY TREATMENT TECHNOLOGIES
COMPARED TO AN NFT SYSTEM (all achieve effluents of 30 mg/I
of BOD and SS).

Conventional
Biological Treatment
Water
Hyacinth
NFT
Area Needs, ha
2
6
1
Energy Production,
Cents/3785 i (1000 gal)
0 to 4
0 to 8
0
By-Product Value
Cents/3785 I (1000 gal)
0
0
40
Sludge for Disposal
Metric tonnes (wet) per
year for 3800 m /d flow
(1 MGD)
% plant material
4600
0
19,100
87
5,200
4
Sludge Disposal Costs
Cents/3785 !¦ (1000 gal)
7
25
10
Note: Tertiary NFT would involve an additional 5 ha, capital cost
a$3,000,000/3875£/d (1 MGD), annual value of products ° $1,000,000.
297

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B. THE NFT AS A TERTIARY TREATMENT FACILITY
The use of the NFT for complete treatment of and/or nutrient recovery from
sewage is a possibility which was not tested in this study. It is likely
that the incorporation of several supporting unit processes and concepts
would be useful in an innovative application of the NFT for tertiary
treatment. The nutrient removal section of the NFT would require a minimum
area of approximately 10 hectares per 10,000 population. In areas of the
country where freezing weather is not a problem, this section would not
require greenhouse protection. In areas south of Pennsylvania it is
possible that the heat in the wastewater would minimize heating
requirements.
Except under the higher light conditions, the nutrient removal from this
system would not meet stringent effluent requirements. Therefore, the
nutrient removal alternatives would have to consider the installation of
either nutrient recovery or nutrient destruction alternatives such as
nitrification/denitrification and/or other chemical alternatives. The
estimated quality with and without the NFT nutrient polishing section and
with the additional unit process and removal of nutrients is shown in Table
9.2. Additional research would be required to confirm these values.
The use of the NFT as a nutrient removal and polishing system raises
numerous questions that can be only partially answered by this study. There
may be significant opportunities for cost recovery through commercial sales
of biomass products from this section. The growth of plants in a low
nutrient environment may limit the number of species that can be produced in
this section. However, there are plant production techniques that can
eliminate substrate composition imbalances for plants. For example, for
many plants foliar feeding (the application of nutrients directly to the
leaf) can be utilized. This would enable high value crops to be produced in
a nutrient stripping NFT, but at an additional cost for adding nutrients.
The economics of using the NFT as a nutrient recovery system remain to be
defined. Although there are large greenhouses in commercial operation, the
sizes proposed here are far larger than any current commercial operation.
The economics, however, are attractive since the capital investment may be
limited to around $54/m2 ($5/ft^) and the return per year may be one-third
of this, depending on the value of the crop. The total capital investment
for the nutrient stripping NFT would exceed $6 million for a flow from
10,000 people. If, however, a significant portion of this facility could be
used to produce commercially viable products, it may be an economically
attractive alternative.
298

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TABLE 9.2. ESTIMATED WATER QUALITY TO BE PRODUCED BY THE PROPOSED WATER RECLAMATION FACILITY.
(Values In mg/£ except as noted).
After Nutrient
Characteristic	After Nutrient Removal/Polishing
Raw Sewage	At Nutrient	Removal/	Additional Unit
(degrltted)	Removal NFT Polishing NFT	Process
Solids
Suspended	200
Dissolved*	500
Settleable, m£/liter	10
Organlcs
BOD	200
COD	500
Nutrients
Nitrogen, Total	40
Phosphorus, Total	10
Heavy Metals	0.1
Toxic Organlcs, ppb
Volatlles (such as chlorlform)	200
Non-volatlle8 (such as PCB)	10
10	<1 <1
150	100	100
0.5	0 0
20	<1 <1
50	<50	<50
20	<10 <0.1
7	5 <0.1
0.01	<0.01	<0.005
20	<5 <5
3	<2 <2
*Plu8 background salts In tap water.

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La)
H
ro
§
2 A 6 10 ? 6 2 6
8 HOUR 8AMPUNQ INTERVALS
10 Z 6
Figure A-l, Nitrogen and phosphorus removals from Ceneral Electric NFT unit
containing Watercress.

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A 6 10 2 6 10 2 A 6 |0 2 6 2 6 10 2 6
6 HOUR 3 AM RUNG INTERVALS
Figure A-2, Nitrogen and phosphorus removals from General Electric NFT unit
containing Napier Grass.

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U)
H
-p-
& 40
<0 |0-
• 10
e io
a HOUR SAMPLING INTERVALS
Figure A-3. Nitrogen and phosphorus removals from General Electric NFT unit
containing Coastal Bermuda Crass.

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APPENDIX B
This appendix presents data developed in Hanover, New Hampshire, using
domestic sewage. It includes data from both the CRREL and Hanover
greenhouses. Tables B.l through B.18 present mean values for variables
tested during each loading condition. The complete data set is presented in
three chronological sets. The first set (Figures B.l to B.27) shows data
from the early experiments in a 7.4 m system in the CRREL greenhouse. The
second set (Figures B.28 to B.58) shows data from later experiments in that
same system. The last set (Figures B.59 to B.113) shows data from the
27.9 m system at the Hanover treatment plant.
315

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TABLE B.l. LOADING CONDITION PARAMETERS TESTED IN PILOT SCALE NFT SYSTEMS IN
NEW HAMPSHIRE USING DOMESTIC WASTEWATER. CONDITION NUMBERS ARE
GUIDES TO SPECIFIC VARIABLE DATA, WHICH FOLLOWS.
Target Loading	Condition
Rate	Dates
Condi-
tion
No.
Area
Loading
Rate
cm/d
Weir
Loading
Rate
m3/m-d
Start
End
System
Area
m
Plant
Species
Application
Mode
1
5.1
0.62
11/16/79
12/2/79
7.4
Canary Grass
Intermittent
2
4.0
0.48
12/19/79
12/27/79
7.4
Canary Grass
10 min on
50 min pause
3
10.2
1.25
12/31/79
1/11/80
7.4
Canary Grass
10 min on
50 min pause
4
10.2
1.25
1/14/80
1/18/80
7.4
Canary Grass
8 hr on
16 hr pause
5
10.2
1.25
1/19/80
1/30/80
7.4
Canary Grass
20 min on
40 min pause
12 hr/d
6
5.1
0.62
1/31/80
2/8/80
7.4
Canary Grass
10 min on
50 min pause
12 hr/d
7
20.3
2.48
2/9/80
2/13/80
7.4
Canary Grass
20 min on
40 min pause
18 hr/d
8
5.1
0.62
2/15/80
4/11/80
7.4
Canary Grass
5 min on
50 min pause
9
10.2
1.24
4/12/80
6/13/80
7.4
Canary Grass
10 min on
50 min pause
10
20.3
2.48
6/14/80
6/30/80
7.4
Canary Grass
20 min on
40 min pause
11
6.9
2.49
2/13/81
3/20/81
27.9
Phragmites*
Cucumbert
Canary Grass §
Continuous
12
10.2
3.74
4/13/81
5/8/81
27.9
Cattail/Buirush*
Phragmitest
Canary Grass§
15 min on
15 min pause
13
20.3
7.47
5/9/81
5/22/81
27.9
Cattail/Buirush*
Phragmitest
Canary Grass§
30 min on
30 min on
*Unit 1, tUnit 2, §Unit 3.
316

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TABLE B.2. SEWAGE FLOW APPLIED AND EFFLUENT FLOW FROM NFT PILOT SCALE
SYSTEMS AT THE CRREL AND HANOVER GREENHOUSES. STANDARD
DEVIATIONS ARE SHOWN IN PARENTHESES.
Condition Number of	Unit 1	Unit 1	Unit 2 Unit 3
Number Determinations Influent Effluent Effluent Effluent
1
11
352
(77)



2
1
2650
—
—
—
3
6
959
(501)
—
—
—
4
2
694
(96)
—
—
—
5
4
649
(216)
—
—
—
6
3
310
(107)
—
—
—
7
2
1090
(390)
—
—
—
8
55
353
(108)
340
(89)
—
—
9
39
746.2
(200)
701
(160)
—
—
10
10
2069
(647)
1926
(644)
—
—
11
17
1565
(878)
—
—
—
12
17
3729
(1905)
—
—
3458
(1802)
13
12
5816
(951)
—
—
5563
(952)
317

-------
TABLE B.3. BIOCHEMICAL OXYGEN DEMAND CONCENTRATIONS (mg/Jl) IN PILOT
SCALE SYSTEMS TREATING DOMESTIC SEWAGE IN NEW HAMPSHIRE.
STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Actual Mass
Condition Loading Rate Number of Unit 1 TTnit 1 Unit 2 Unit 3
Number	kg/ha-d Determinations Influent Effluent Effluent Effluent
1






z
3
172
4
133.2
33.6





(27.1)
(19.5)
——
——
4
109
2
116.0
95.0
__
__



(4.2)
(3.4)


5
103
2
117.0
38.7
—
—



(17.0)
(21.6)


6
62
3
148.0
42.3
__

7


(48.9)
(5.1)


8
58
23
121.3
21.8





(61.0)
(12.3)


9
101
12
99.9
21.4

— —



(50.6)
(10.2)


10
133
3
51.0
9.6

_



(16.9)
(7.6)


11
67
4
119.4
71.0
39.5
10.7



(23.6)
(6.0)
(19.8)
(5.5)
12
182
4
136.1
105.2
74.9
38.8



(28.8)
(21.1)
(17.1)
(9.2)
13
272
3
130.7
88.3
70.8
53.4



(9.2)
(15.5)
(6.2)
(9.4)
318

-------
TABLE B.4. SOLUBLE BIOCHEMICAL OXYGEN DEMAND CONCENTRATIONS (mg/A) IN
PILOT SCALE SYSTEMS TREATING DOMESTIC SEWAGE IN NEW
HAMPSHIRE. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Actual Mass
Condition Loading Rate Number of Unit 1 Unit 1 Unit 2 Unit 3
Number	kg/ha-d Determinations Influent Effluent Effluent Effluent
2
3
5
A
—
—
—
—
—
—
0
7
8
9
—
—
—
—
—
—
10
11
44
2
78.6
(1.9)
45.7
(6.1)
32.4
(13.6)
10.0
(2.2)
12
120
4
89.5
(9.9)
79.6
(12.9)
52.0
(14.2)
33.4
(7.9)
13
156
3
74.7
(9.5)
74.7
(5.5)
53.7
(8.8)
40.8
(8.5)
319

-------
TABLE B.5. TOTAL ORGANIC CARBON CONCENTRATIONS (mg/*) IN PILOT SCALE
SYSTEMS TREATING DOMESTIC SEWAGE IN NEW HAMPSHIRE. STANDARD
DEVIATIONS ARE SHOWN IN PARENTHESES.
Actual Mass
Condition Loading Rate Number of Unit 1 Unit 1 Unit 2 Unit 3
Number	kg/ha-d Determinations Influent Effluent Effluent Effluent
1
2
3
7
8
132
75
84
48
84
13
102.0
(8.5)
80.5
(41.7)
95.5
(13.4)
101.1
(36.9)
83.2
(36.9)
27.5
(7.7)
42.0
(2.8)
(10.6) (4.9)
29.3
(9.0)
43.2
(16.9)
10
11
12
13
49
101
100
3
4
5
6
86.5
(21.8)
75.7
(13.6)
48.0
(21.1)
51.4
(4.4)
63.2
(10.0)
45.6
(11.1)
46.0
(8.7)
50.7
(8.5)
35.6
(14.2)
24.0
(2.7)
34.3
(7.1)
24.7
(9.5)
320

-------
TABLE B.6. CHEMICAL OXYGEN DEMAND CONCENTRATIONS (mg/i) IN PILOT SCALE
SYSTEMS TREATING DOMESTIC SEWAGE IN NEW HAMPSHIRE. STANDARD
DEVIATIONS ARE SHOWN IN PARENTHESES.
Actual Mass
Condition Loading Rate Number of Unit 1 Unit 1 Unit 2 Unit 3
Number	kg/ha-d Determinations Influent Effluent Effluent Effluent
2
3
4
5
6
—
—
—
—
—
—
7
8
9
—
—
—
—
—
—
10
—
—
—
—
—
—
11
133
3
236.3
163.3
132.0
59.0



(4.5)
(8.0)
(21.3)
(19.5)
12
372
5
278.2
235.4
192.6
146.4



(40.9)
(35.7)
(32.6)
(16.2)
13
556
4
266.5
218.2
190.7
154.5



(13.0)
(3.4)
(7.0)
(10.1)
321

-------
TABLE B.7. SOLUBLE CHEMICAL OXYGEN DEMAND CONCENTRATIONS (mg/I) IN
PILOT SCALE SYSTEMS TREATING DOMESTIC SEWAGE IN NEW
HAMPSHIRE. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Actual Mass
Condition Loading Rate Number of Unit 1 Unit 1 Unit 2 Unit 3
Number	kg/ha-d Determinations Influent Effluent Effluent Effluent
1
2
3
4
5
6
7
8
10
11
94
3
167.0
107.7
92.3
56.0



(3.6)
(4.0)
(18.6)
(17.4)
12
272
5
203.2
182.0
156.0
128.0



(20.2)
(20.8)
(15.8)
(13.0)
13
395
4
189.5
169.2
142.5
126.0



(6.4)
(5.1)
(6.6)
(3.8)
322

-------
TABLE B.8. SUSPENDED SOLIDS DEMAND CONCENTRATIONS (mg/i) IN
PILOT SCALE SYSTEMS TREATING DOMESTIC SEWAGE IN NEW
HAMPSHIRE. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Actual Mass
Condition Loading Rate Number of Unit 1 Unit 1 Unit 2 Unit 3
Number	kg/ha-d Determinations Influent Effluent Effluent Effluent
1
2
3
10
11
117
73
68
47
206
46
110
175
20
3
3
1
20
13
3
3
90.2
(20.5)
78.3
(14.2)
77.7
(18.0)
111.
(93.8)
140.0
97.2
(93.3)
108.9
(85.1)
62.5
(50.2)
35.1
(9.4)
15.8
(11.6)
30.1
(21.7)
32.0
(31.3)
26.2
(19.8)
36.2
33.8
(16.2)
33.5
(19.2)
4.3
(0.3)
27.1
(5.1)
17.2
(2.3)
4.1
(1.5)
12
57
42.4 31.7 17.3 7.3
(12.9) (14.7) (3.9) (1.6)
13
98
47.2
(7.4)
24.1
(2.9)
26.2
(4.8)
16.4
(3.5)
323

-------
TABLE B.9. VOLATILE SUSPENDED SOLIDS DEMAND CONCENTRATIONS (mg/i) IN
PILOT SCALE SYSTEMS TREATING DOMESTIC SEWAGE IN NEW
HAMPSHIRE. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Actual Mass
Condition Loading Rate Number of Unit 1 Unit 1 Unit 2 Unit 3
Number	kg/ha-d Determinations Influent Effluent Effluent Effluent
X
0






£.
3
54
4
42.0
(39.1)
10.7
(10.2)
—
—
4
65
3
69.7
(13.0)
15.9
(1.7)
—
—
5
46
3
52.7
(24.9)
15.6
(4.4)
—
—
6
35
3
83.1
(63.6)
17.5
(7.8)
—
—
7
180
1
122.0
20.8
—
—
8
29
6
59.9
(81.2)
19.0
(7.8)
—
—
9
108
4
107.5
(34.8)
14.2
(12.4)
—
—
10
174
3
62.3
(22.8)
2.9
(0.1)
—
—
11
24
4
3
42.3
(17.6)
24.4
(4.7)
15.3
(2.2)
3.8
(1.2)
12
50
5
37.4
(11.7)
26.7
(11.8)
15.8
(3.5)
6.8
(1.3)
13
87
4
41.8
(6.1)
20.4
(2.2)
23.4
(4.1)
15.5
(3.1)
32k

-------
TABLE B.10. TOTAL KJELDAHL NITROGEN CONCENTRATIONS (mg/Jl) IN PILOT
SCALE SYSTEMS TREATING DOMESTIC SEWAGE IN NEW HAMPSHIRE.
STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Actual Mass
Condition Loading Rate Number of Unit 1 Unit 1 Unit 2 Unit 3
Number	kg/ha-d Determinations Influent Effluent Effluent Effluent
1
2




__
__
3
4
44.5
1
47.4
40.2
__ __
_ _
5
41.2
3
47.0
(2.2)
39.2
(1.6)
—
—
6
20.2
3
48.3
(2.9)
31.8
(1.4)
—
—
7
67.9
2
46.1
(3.5)
36.6
(2.7)
—
—
8
17.5
13
36.7
(12.2)
27.3
(6.4)
—
—
9
10
11
27.3
5
27.1
(7.2)
22.6
(6.4)


13.0
3
23.1
(2.5)
21.8
(2.0)
20.5
(1.7)
16.9
(1.3)
12
36.4
6
27.2
(3.4)
25.7
(2.3)
23.1
(1.8)
20.4
(2.4)
13
44.4
5
6
21.3
(6.1)
19.6
(2.2)
18.6
18.8
(2.8) (2.7)
325

-------
TABLE B.ll. NITRATE NITROGEN CONCENTRATIONS (mg/&) IN PILOT SCALE
SYSTEMS TREATING DOMESTIC SEWAGE IN NEW HAMPSHIRE.
STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.

Condition
Number of
Unit 1
Unit 1
Unit 2
Unit 3
Number
Determinations
Influent
Effluent
Effluent
Effluent
1
1
0.0
16.8
—
—
2
3
2
0.0
0.4




—
(0.1)


4
3
0.0
4.2
—
—


	
(4.7)


5
4
0.0
0.4
——
__


—
(0.2)


6
3
0.0
2.9
__



—
(3.4)


7
3
0.0
0.9

——


—
(0.7)


8
13
0.0
4.1
——
	


—
(2.3)


9
5
0.0
1.4
	
	
1 n


(1.6)


1U
11
4
0.0
0.0
0.0
0.26


—
—
—
(0.21)
12
6
0.0
0.0
0.0
0.0
13
5
0.0
0.0
0.0
—

8
—
—
—
0.0







326

-------
TABLE B. 12. AMMONIUM NITROGEN CONCENTRATIONS (mg/Jt) IN PILOT SCALE
SYSTEMS TREATING DOMESTIC SEWAGE IN NEW HAMPSHIRE.
STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.

Condition
Number of
Unit 1
Unit 1
Unit 2
Unit 3
Number
Determinations
Influent
Effluent
Effluent
Effluent
1
1
55.3
23.0
—,
—
2
3
2
40.0
36.6




(4.9)
(3.7)


4
3
36.2
30.1
—



(2.3)
(3.8)


5
4
37.8
32.8
—
__


(3.3)
(1.5)


6
3
35.3
25.2
——
	


(1.9)
(3.7)


7
2
35.3
29.9
__



(3.0)
(3.0)


8
13
29.0
19.8




(9.1)
(6.9)


9
5
21.6
18.1
—
__
1 fi

(4.7)
(6.3)


1U
11
4
21.4
20.2
20.5
17.5


(2.7)
(3.0)
(3.4)
(2.9)
12
6
21.8
21.8
21.5
21.7


(2.2)
(2.6)
(3.2)
(2.8)
13
5
20.7
21.3
20.0
21.3


(2.5)
(3.1)
(3.5)
(3.5)

327

-------
TABLE B.13. TOTAL PHOSPHORUS CONCENTRATIONS (mg/A) IN PILOT SCALE
SYSTEMS TREATING DOMESTIC SEWAGE IN NEW HAMPSHIRE.
STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Actual Mass
Condition Loading Rate Number of Unit 1 Unit 1 Unit 2 Unit 3
Number	kg/ha-d Determinations Influent Effluent Effluent Effluent
1
2






3
4
7.22
1
7.7
7.3
—
—
5
6.31
3
7.2
(0.5)
5.8
(1.2)
—
—
6
3.77
3
9.0
(0.9)
6.1
(1.1)
—
—
7
12.96
2
8.8
(0.1)
7.6
(1.1)
—
—
8
3.05
12
6.4
(2.3)
5.1
(1.2)
—
—
9
10
11
1.24
5
4.8
(1.6)
4.7
(1.1)


2.97
4
5.3
(0.1)
5.0
(0.2)
4.7
(0.5)
4.1
(0.7)
12
7.62
4
5.7
(0.5)
5.4
(0.6)
5.2
(0.6)
4.8
(0.7)
13
44.4
5
5.8
(0.5)
5.6
(0.6)
5.5
(0.7)
5.5
(0.8)
328

-------
TABLE B.14. SOLUBLE PHOSPHORUS CONCENTRATIONS (mg/A) IN PILOT SCALE
SYSTEMS TREATING DOMESTIC SEWAGE IN NEW HAMPSHIRE.
STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.

Condition
Number
Number of
Determinations
Unit 1
Influent
Unit 1
Effluent
Unit 2
Effluent
Unit 3
Effluent
1
2
3
3
6.5
(0.1)
6.1
(0.5)

—
4
1
9.3
6.5
	
—
5
6
3
7.7
(0.7)
8.0
(0.4)
——
——
7
8
13
4.8
(1.5)
4.3
(1.1)
	
	
9
10
11
5
4.1
(1.0)
4.4
(1.0)


4
4.6
(0.3)
4.4
(0.2)
4.2
(0.5)
3.8
(0.9)
12
6
4.7
(0.5)
4.7
(0.5)
4.6
(0.6)
4.6
(0.7)
13
5
4.5
(0.4)
4.7
(0.5)
4.7
(0.6)
4.8
(0.7)
329

-------
TABLE
B.15. pH IN PILOT
SCALE SYSTEMS
I TREATING
DOMESTIC SEWAGE IN

NEW HAMPSHIRE. STANDARD
DEVIATIONS
ARE SHOWN IN

PARENTHESES.




Condition
Number of pH
Unit 1
Unit 1
Unit 2 Unit 3
Number
Determinations
Influent
Effluent
Effluent Effluent
1
2
8.0
7.7



(0.8)
(0.4)

z
3
7
7.3
7.3



(0.2)
(0.1)

4
3
7.3
7.2
_


(0.2)
(0.2)

5
6
7.3
7.2
	 	


(0.2)
(0.1)

6
4
7.1
7.0
	 	


(0.2)
(0.1)

7
3
7.3
7.1
	 	


(0.2)
(0.1)

8
37
7.2
6.9
	 ——


(0.4)
(0.3)

9
28
6.9
6.9
—— —_


(0.3)
(0.2)

10
6
7.2
7.1
	 	


(1.2)
(1.1)

11
4
7.2
7.4
7.3


(0.6)
(0.1)
(0.1)

3


7.4
12



(0.1)
X fa
13
—
—
—
—

330

-------
TABLE B.16. FECAL COLIFORMS CONCENTRATIONS (NUMBER/100 m*) IN PILOT SCALE
SYSTEMS TREATING DOMESTIC SEWAGE IN NEW HAMPSHIRE. STANDARD
DEVIATIONS ARE SHOWN IN PARENTHESES.

Condition
Number
Number of pH
Determinations
Unit 1
Influent
Unit 1
Effluent
Unit 2
Effluent
Unit 3
Effluent
1
2
3
1
0.9
0.4
—,
—
4
1
1.6
0.0
—
—
5
1
1.8
0.8
—
—
6
7
1
2.1
0.7
—
—
/
8
4
7.9
(2.8)
0.6
(0.2)
—
—
9
4
6.0
(1.6)
1.4
(1.5)
—
—
10
11
1
3.6
1.0
MM
	
12
13
—
—
—
	
—
331

-------
TABLE B.17. TURBIDITY (JTU) IN PILOT SCALE SYSTEMS TREATING DOMESTIC
SEWAGE IN NEW HAMPSHIRE. STANDARD DEVIATIONS ARE SHOWN IN
PARENTHESES.

Condition
Number
Number of pH
Determinations
Unit 1
Influent
Unit 1
Effluent
Unit 2
Effluent
Unit 3
Effluent
1
O
1
58.0
27.0
—
—
Z
3
7
48.7
(13.7)
13.5
(9.9)
—
—
4
3
38.0
(4.0)
17.0
(2.0)
—
—
5
6
40.7
(4.8)
18.7
(1.6)

—
6
4
51.0
(16.9)
13.6
(3.2)
—
—
7
2
49.5
(9.2)
25.5
(3.5)
—
—
8
37
44.8
(18.0)
13.2
(6.3)
—
—
9
28
51.8
(34.3)
14.8
(8.8)
—
—
10
11
6
28.8
(12.1)
5.6
(6.1)


12
13
—
—
—
—
—

332

-------
TABLE B. 18. SEWAGE TEMPERATURE (°C) IN PILOT SCALE SYSTEMS TREATING
DOMESTIC SEWAGE IN NEW HAMPSHIRE. STANDARD DEVIATIONS
ARE SHOWN IN PARENTHESES.

Condition
Number
Number of pH
Determinations
Unit 1
Influent
Unit 1
Effluent
Unit 2
Effluent
Unit 3
Effluent
1
2
3
10.3
(0.6)
13.5
(1.8)
—
—
3
3
10.7
(0.6)
15.0
(1.7)
—
—
4
5
9.1
(0.7)
14.2
(2.0)
—
—
5
4
7.6
(2.4)
12.8
(0.5)
—
—
6
7
3
8.8
(0.3)
12.0
(1.0)
-¦ ¦
—1~
/
8
37
9.2
(0.9)
13.8
(2.1)
—
—
9
27
12.2
(1.5)
15.5
(1.5)
—
—
10
6
16.0
(1.2)
17.2
(0.4)
—
—
11
5
14.0
(0.7)
24.0
(3.6)
25.8
(4.8)
24.8
(6.0)
12
7
16.0
(2.7)
21.4
(4.5)
23.0
(4.8)
20.7
(3.8)
13
9
18.2
(1.9)
21.8
(2.2)
22.6
(2.6)
21.5
(2.8)

333

-------
?70 0
ELiQQ
2-1 0 0
130 0
o<
» '1 3 0 0
5
rH '120 0
b
30 0
E0 0
30 0
0
0 -10 EQ 30 !-i0 5 0 EO 70 B0 3 0 -100
Time, days
Figure 3.1. Daily influent sewage flow to the CRREL NFT
unit in Phase 0, beginning on November lo, 19T9-
33U

-------
no
e
*
Q
O
«
E50-J-
sss..
20 0 - -
•1 75..
150-
it.
1S5--
-100..
?5-
50-
as--
0 —
0

	1-
-1 3
20 2E 33 33 l,e
—i—
53
ES
		Time, days
Figure B.2. Biochemical oxygen demand concentration
of influent sewage flow to the CRREL
NFT unit in Phase 0. First day of
analysis was December 7, 1979.
25 0
225-.
200 -¦
•175..
'1 5 0 - -
155-'
1 00-
75-
50
25
0
0
Figure B.3.
rj'
a
"o——^
t	i	1	>
H	b
•13 20 2E 33 35 >-.E 52 55 E5
Time, days
Biochemical oxygen demand concentration of
effluent sewage from the CRREL NFT unit
in Phase 0. First day of analysis was
December 7, 1979-
335

-------
-1501
'135-



1E0-
1 05 -
50 !
75-
50-
«-i5 ¦
r'
	-G3
\ K
\ / \
\ / ^
V
,~
		
30-



-1 5-



0 -
1
t t 1 t 1
—1 I 1
0	7 '11 m -1B 21 E5 SB 3E 35
Time, days
Figure 3.U. Total organic carbon concentration of
influent sewage flow to the CRREL NFT
unit in Phase 0. First day of analysis
was January 2, 1980.
Time, days
Figure B.5. Total organic carbon concentration of
effluent sewage from the CRREL NFT unit
in Phase 0. First day of analysis was
January 2, 1980.
336

-------
1 Q0
3 0
50
70
B0
5 0
Li 0
30
30
'1 0
0
0 7 m E-1 EB 35 I-.E *-i3 5B E3 70
Tine, days
Figure B.6. Total Kjeldahl nitrogen concentration of
influent sewage flow to the CRREL NFT
unit in Phase 0. First day of analysis
was December 12, 1979-
•1 00
30
B 0
70
50
50
L-.0
30
E 0
•1 0
0
0 7 '1 !-i S'l E B 35 LiS 1-.3 55 B3 70
Time, days
Figure B.7. Toxal Kjeldahl nitrogen concentration of
effluent sewage from the CRREL NFT unix
in Phase 0. First day of analysis was
December 12, 1979-
337

-------

S0T

5 l-i 5 I

L-.B --

«-.a-
to
£
35-
r*
s
30-
+
Si-.-.
ac
s
•1 B
1 a -•
E --
Figure B.8.
Ammonium nitrogen concentration of
influent sewage flow to the CRREL
NFT unit in Phase 0. First day of
analysis was November 29, 19T9-
B 0
Id
•15 E3 3Q 3B	S3 E8 S3 7E
Figure B.9.
Time, days
Ammonium nitrogen concentration of
effluent sewage from the CRREL NFT
unit in Phase 0. First day of
analysis was November 29, 1979-
338

-------
50
e
co
3S +
d r +
•1 ~ •
1 Li«
3
hJ ffi-
0
{ !_
i i
i i
I i
h—a—f-
~i a ~ (CD ~—>h nn m n nc rp-
•1 s
H ~J Ht3
5E B"i
B0
Fig-are B.10.
Time, days
Nitrate concentration of influent
sewage flew to the CRREL NFT -unit
in Phase 0. First day of analysis
was November 29, 1979*
oj
to
s
I
on
o
i	—a " it- g cpnffr-~"	
B8
Time, days
Figure B.ll. Nitrate nitrogen concentration of
effluent sewage from the CRREL NFT
unit in Phase 0. First day of
analysis was November 29, 1979*
339

-------
130
£
a<
EH
0
1 Q -13
¦10 20 23 25
Figure B.12.
Time, days
Total phosphorus concentration of
influent.sewage to the CRREL unit
in Phase 0. First day of analysis
was January 18, 1980.
Time, days
Figure B.13. Total phosphorus concentration of
effluent sewage from the CRREL NFT
unit in Phase 0. First day of
analysis was January 19, 1980.
3^0

-------
I
•; .i.
"—
to	l
Pu
I
O
PL,
~
	VJ	_c
fa
3 i
P i
8 -i-

•i 0 15 s a
30 3:
L-i t) '-i 5 tl
Figure B.lU.
Time, days
Orthophosphate concentration of
influent sewage to the CRREL NFT
unit in Phase 0. First day of
analysis was December 12, 19T9-
15r
1 - f
iai
to
e
-3"
O
cu
3
~
S
3
3
2 +
0
-1 0
_-9—«---
-s-
•15 30 35 30 35 >-.0 ^5 50
Figure B.15.
Time, days
Orthophosphate concentration of
effluent sewage from the CRREL NFT
unit in Phase 0. First day of
analysis was Decem'cer 12, 1979*
3^1

-------
uo
s
m
CO
E-1
ESS y
225 --
20 0-
•175-
'150-
'125-
'1 0 0-
75-
50-
25-
3 --
0
I
¦a'
o-er'
a
JU AA
s .•
ti
h Yi

•m
S3 70
M 20 35 L-(2 t-tS 55
Time, days
Figure B.l6. Total suspended solids concentration
of influent sewage to the C3REL NFT
unit in Phase 0. First day of
analysis was December 7, 1979*
25 0
225
20 0
175
1 150
ui" 13 5
H 1 0 0
75
50
25
0 7 m 2-1 2 B 3 5 li2 i-,5 5 5 5 3 70
Time, days
Figure 3.17. Total suspended solids concentration
of the effluent sewage from the
CRREL NFT unit in Phase C. Firs^
day of analysis was December 7, 1979.

3b2

-------
1 50
1 Eh
-line, days
Figure B.l8.
Volatile suspended solids concentration
of influent sewage to the CRREL NFT
unit in Phase 0. First day of analysis
was December 7, 1979*
-1B0-|
1 l-l .


•iaa-


•1-1 a.


3 B -


B0-


B1-! -


L-.B-

w
32-

	...

1				
0-
	1	
—1	1 1 	1	1	1 1 1	
0
•1 a-1
aa as >-.a
Time, days
"iS BE S3 70
Figure B.19>
Volatile supsended solids concentration
of effluent sewage from the CRREL NFT
unit in Phase 0. First day of analysis
was December 7, 1979.
3^3

-------
1 0 -
9 t
I
I
5 -I	1 I	! I I I	1	1	1
0 1 fcJ 20 30 '—i kJ 5 0 G0 r"0 3 0 9 0 100
Time, days
Figure 3.20. pH of influent sewage to the CRRZL NFT
uni"£ in Phase 0. First day of analysis
was November 20, 1979•
•1 0
10 E0 30 '—10 50 E0 70 B0 3 0 100
Time, days
Figure B.21. pH of effluent sewage from the CRREL
NJT unit in Phase 0. First day of
analysis was November 20, 1979-
3UU

-------
Id
«-1
•11 1-i a a - i
Time, days
25 2B 35 35
Figure B.22,
Temperature of influent sewage to the
CRREL NFT unit in Phase 0. First day
of analysis was November 3C, 1979.
o
o
o
20 j
•1 b ..
-1 E--
mi
I
-1 2 »
r---
5 '10 +
05
^ S 1
ft
s a ±

E-i
x

if
i ¦¦ "> R f x a
41 S t "\i \ I *
a
i
""
a i
8 Li
•1 1 -1 !-i B 2'1 25 59 3d
Figure B.23.
Time, days
Temperature of effluent sewage from the
CRREL NFT unit in Phase 0. First day
of analysis was November 30, 19T9-
3h5

-------
Time, days
Figure B.2U. Turbidity of influent sewage to the
CRREL NFT unit in Phase 0. First
day of analysis was November 30, 1979.
1 9 0
3 0
7 0
S S --
I
= * +
1.0.1
31 j I	f I
'f-..	I !	/ ¦
^ ® f ""EL	„	_	j i, J3.
•-T3—J33	E',	,S#I
•1 0 4-	•
-i ci	a
0 \	1	1	1	1-
0 b -is sl-. aa ma ee £¦-, ?e 50
Time, days
Figure B.25- Turbidity of effluent sewage from
the CRREL NFT unit in Phase 0.
First day of analysis was November 30,
1979.
3^6

-------
Time, days
Figure B.26. Numbers of fecal colifcrns in influent
sewage to the CRREL N7T unit ir. Phase 0.
Firsx day of analysis was January 9,
1980.
Time, days
Figure B.27. Numbers of fecal coliforns in effluent
sewage from the CRREL NFT unit in
Phase 0. First day of analysis was
January 9, 1979-
3^7

-------
Time, days
Figure B.28. Daily influent sewage flow to the CRREL
UFT unit in Phase 1, beginnong on
February 15 » 1980.

>
o
2=00 0 _
E70 8 -¦
i
S1—iQ 0 f
2-1 0 0 -
¦1 see --
1500 --
•1 0 --
S0e{
rr: 0 t'l 4-
nc i, ri.
! .= Ir(.
50 0
¦S
0
on
VI

•:ri i
I I til
%

Figure B.29.
7 5 50 •;! 05 -I 5 0 -1 35
?ime, days

Daily effluent sewage flow from the CRREL
NFT unit in Phase 1, beginning on
February 16, 1980.
3^8

-------
EB0
EE 5
E0 0
-17 5
-1 50
'1 E5
'100
' 75
50
E5
0
0 m E7 m 5•—i EB B1 55 i0B 'I EE 135
Time, days
Figure B.3C. Biological oxygen demand concentrations
of influent sewage to the CREEL NFT
unit in Phase 1. First day of analysis
was February 15, 1980.
E0 0 - •
100-.
?5-
50--
0 11-1 E"? m 5>1 EB B1 55 10B1EE135
Time, days
Figure B.31. Biological oxygen demand concentraxions
of effluent sewage from the CRREL NFT
unit in Phase 1. First day of analysis
was February 15, 1980.
3^9

-------
'1 70
\
1 -1 3
~'I -10h 117-130
Figure B.32.
Time, days
Total organic carbon concentrations of
influent sewage to the CRREL NFT uni"C
in Phase 1. First day of analysis was
February 15, 1980.
'1 T7 0 j-
-1 53 --
•1 3E -•
•1 -1 3
0	I	I	1	1	1	1	1	1	I	
Q -13 EE 33 5 E E5 7B 3-1 •10^17-130
Tine, days
Fig-ore B.33. Total organic carbon concentrations
of effluent sewage from the CRREL
NFT uni~ in Phase 1. First day of
analysis was February 15, 1980.
350

-------

70-r

S3-

55-

^a-


no
L-.a --
S
el
*
35-
a

EH
5B -¦

2-1 -¦

m--

T7

0 --

0
Time, days
Figure B.3U. Total nitrogen concentrations of
influent sewage to the CRREL NFT
unit in Phase 1. First day of
analysis was February 15, 1980.
70
so
e
V !»-%
Time > days
30
Figure B-35- Total nitrogen concentrations of
effluent sewage from the CRREL NFT
unit in Phase 1. Firsx day of
analysis was February 15, 1980.


-------
0 3
•IB 57 3E l-i 5 51-. S3 75 B-1 30
Time, days
Figure B.36. Ammonium nitrogen concentrations of
influent sewage to the CREEL NFT
unit in Phase 1. First day of
analysis was February 15, 1980.

50 T

1-.5 --

"—(0 --

33 --
to
30"
r\
s
as--
+
-a-
X
3
50 ..
•15-
•10--
5 --
V
0
1B 37
3E I-.5 S1—i
Time, days
S3 7S B-1 30
Figure B.37- Ammonium nitrogen concentrations of
effluent sewage from the CRREL NFT
unit in Phase 1. First day of
analysis was February 15, 1980.
352

-------
0 in do ogoDoo ~ omoaio	ma o ~ a a ~
0 3 -IB 5? 3E i-iS 5«-. S3 75 Bt 30
Time, days
Figure B.38. Nitrate nitrogen concentrations of influent
sewage to the CRREL NFT unit in Phase 1.
First day of analysis was February 15, 1980
1 Q
3
B
7
E
5
3
5
•1
0
0 3 '1B 57 3E US 51-. E3 75 B-1 30
Time, days
Figure B.39. Nitrate nitrogen concentrations of effluent
sewage from the CRREL NFT unit in Phase 1.
First day of analysis was February 15, 1980
353

-------
00
e
Pu
H
V
\
N
0 5
7S 01 30
IB E? 3E <-.5 5"-, E3
Time, days
Figure B.40. Total phosphorus concentration of influent
sewage to the CRREL NFT unit in Phase 1.
First day of analysis was February 22, 1980.
60
E
H
3
P
-1
a
-j a
•1 B
•a a-i as
Time, days
Figure B.41. Total phosphorus concentration of influent
sewage from CRREL NFT unit in Phase 1.
First day of analysis was February 22, 1980.
3?4

-------
1	0
3
B
7
E
5
i-i
3
3
'1
0
0 3 "IB S7 3E liS S1-! S3 73 B-1 30
Time, days
Figure B.42. Orthophosphate concentration of influent
sewage to the CRREL NFT unit in Phase 1.
First day of analysis was February 15, 1980.
'1 0
3
B
7
E
5
L-.
3
2
1
0
0 3 -13 37 3E 1-.5 Si-. E3 73 B-1 30
Time, days
Figure B.43. Orthophosphate concentration of effluent
from the CRREL NFT unit in Phase 1. First
day of analysis was February 15, 1980.
.X
1V14
R
I

t Fx ,
\ /



-i	1	1	1	t	1	i	i	1-
355

-------
Time, days
Figure B.44. Total suspended solids concentration of
influent sewage to the CKREL NTT unit in
Phase 1. First day of analysis was February
15, 1980.
3E 0
3y\ -
:3 -•
= •1 :"l 1
i
¦1 r 5 |
1 0 i
1 Q C
~F 0 4-	i'n '7
\	9 "3 o _ ,?i • i
-	^ i	a r L __ i \ /' p p tor,
-	J	d 13 ^ ^ I
^ J	1	I	1	1	1-
-42ES31-I
0 -13 EE 33 5 3 EE 70 3-1 101-, Mr 130
Time, days
Figure B.45. Total suspended solids concentration of
effluent sewage from the CRREL NFT unit
in Phase 1. First day of analysis was
February 15, 1980.
356

-------
EE @
EtJti -¦
150-
I M 0 _.


33 55 S3 70 3-1 1 0I-, -1 -1 7 1 30
Time, days
Figure B.46. Volatile suspended solids concentration of
influent sewage to the CRREL NFT unit in
Phase 1. First day of analysis was February
20, 1980.
E5 0
EE5--
S00--
•173-.
-150--
-1 S3 -¦
•100-.
75 -•
50 --
2S,A
0


•13 as 33
iS 55 7B 3-1 -1 0h -1 -1 7 -1 30
Time, days
Figure B.47. Volatile suspended solids concentration of
effluent sewage from the CRREL NFT unit.
First day of analysis was February 20, 1980.
357

-------
-1 G
~
B
sc 7
o.
S
5
0 15 30 >-15 B0 75 30 -1 05 1S0 135 -150
Time, days
Figure B.48. pH of influent sewage to the CRUEL NFT unit
in Phase 1. First day of analysis was
February 15, 1980.
-1 0
3
B
a
Q.	_
E
0 15 30 I-.5 B0 7 5 30 -1 05 -1E0 1 3 5 -1 50
Time, days
Figure B.49. pH of effluent sewage from the CRREL NFT
unit in Phase 1. First day of analysis
was February 15, 1980.
358

-------
-1 B -•
-1 E --
-1 a --
0 'ii-i a? m s1-! bs b-i as'iQB-iaa-135
Time, days
Figure B.50. Temperature of influent sewage to the
CRREL NFT unit in Phase 1. First day of
analysis was February 15, 1980.
301
-1 B -¦
-1 B ¦¦
•15-
0 -i L-t a? •—1-*1 S1-! 5B B1 55 'lyS 1 as '135
Time, days
Figure B.51. Temperature of effluent sewage from the
CRREL NFT unit in Phase 1. First day of
analysis was February 15, 1980.
359

-------
0 -I	I	I	I	I	I	I	1	1
0 -10 20 30 LI0 50 E0 70 00 90 -100
Time, days
Figure B.52. Daily maximum air temperature in the CRREL
greenhouse in Phase 1. First day of analysis
was February 15, 1980.


i :
•l 8 f
I
B 4-
'JT"iT?Z:ZW	rjiysiQ
' i
I
:3
Z£l
'J
3
II & i
is j
I
•1 M
cr'd -:'J '~t0 by tr.0
S 0 B 0
Time, days
Figure B.53. Daily minimum air temperature in the CRREL
greenhouse in Phase 1. First day of analysis
was February 15, 1980.
360

-------
3 0
b
I
£0
70 E30 30 -100
Figure B.54.
>-i0 50 E0
Time, days
Outside incident radiation at CRREL in
Phase 1. First day of analysis was
February 15, 1980.
361

-------
'1 30
1.-1 Y ¦¦
r
-y0
35 10B 12E -1 35
Figure B.55.
Time, days
Turbidity of influent sewage to the CRREL
NFT unit in Phase 1. First day of analysis
was February 15, 1980.
•1 3 0
• 1 t-,..
3-1 -¦
7B--
55 -¦
5E -•
4k
4-J
•H
•H
3a-
as --
•2
3
H
m ^7 L-,1 gi_ ES 3 1 35 "10 B -13 3 "135
Time, days
Figure B.56. Turbidity of effluent sewage from the CRREL
NFT unit in Phase 1. First day of analysis
was February 15, 1980.
362

-------
23
0 m a? m 51-. eb b-i as 10a -122 -135
Time, days
Figure B.57. Numbers of fecal coliforms in influent sewage
to the CREEL NFT unit in Phase 1. First day
of analysis was February 20, 1980.
0 m 2? i—i-"1 5h EB B-1 35 -10B -1SS -135
Figure B.58,
Time, days
Numbers of fecal coliforms in effluent sewage
from the CRREL NFT unit in Phase 1. First day
of analysis was February 20, 1980.
363

-------
S"r0
Ln?50 4-
i
330 0 --
2350 --
1 30 0 -
rj
I.
!S< n? IL.^
! i' ,1 ! ^7
CI
n
rp
tn?L
ar
:	ii.
I !,	! V
i	i *?
ra I :
n
. ,i ,_m| i|t«?
CD ("-P "j;l J '1
~0SK I
asiLi^—,	h
		
i® ¦*
I i Q 'S
4 f
i t
ir3
a
4L
0 -10 50 30 t-10 5 0 E0 70 ~ 0 30 -100
Time, days
Figure B.59. Daily influent sewage flow to the Hanover
NTT unit, beginning March 2, 1981.
350 0
355 0 I
750 ©
555 0
5700 - ¦
1-.75 0 --
350 0 ...
a5T
3B5 0 -k
1 50 0 --
35 0 ••
0 -
'ill
0
12 f

I

D5| -1
CU
a
•10 30 30
*-10 5 0 50 70
Time, days
Bu 5 0 100
Figure B.60. Daily effluent sewage flow from the Hanover
NFT unit, beginning March 26, 1981.
36U

-------
ESQ
'1B0
-1E0
•1 L-i0
'1 20
•*100
B0
E0
I-.0
50
0
0 B '15 E3 30 3B L-iB B3 B0 BB 75
Time, days
Figure B.61. Biological oxygen demand concentration of
influent sewage to the first unit of the
Hanover NFT in Phase 1. First day of
analysis was March 4, 1981.
E0 0
•1B0
•1E0
m©
•150
'10 0
B 0
G 0
1-.0
E 0
0
0 B -15 EE 30 3B I-.5 53 50 BB 75
Time, days
Figure B.62. Biological oxygen demand concentration of
effluent sewage from the first unit of the
Hanover NFT in Phase 1. First day of analysis
was March 4, 1981. These values also repre-
sent influent concentrations to the second unit.

\ A
365

-------
i_ -.J 0
'1 B 0 ¦ ¦
•150-
'1 1-10..
•1 B 0 - ¦
•100-
B 0 - ¦
50-
t-10--
E O - -/
ij
0	
R
/ \ -A
/ I .•* 4

V ^
£3"
0 B
Figure B.63.
•15 23 30 30 Li5 53 50 SB 75
Time, days
Biological oxygen demand concentrations of
effluent sewage from the second unit of the
Hanover NFT in Phase 1. First day of analysis
was March 4, 1981. These values also represent
influent concentrations to the third unit.
-130-
• 1 5 0--
I
1^0..
•1 S0 --
•1 e 0 - -
i
5 0 -}¦

.-•3
,D
3

•J l-
E0
Time, days
Figure B.64,
Biological oxygen demand concentrations of
effluent sewage from the third unit of the
Hanover NFT in Phase 1. First day of analysis
was March 4, 1981.
366

-------
H	1	1	1	1	1	1	1	1	A
0 7 I1-! E1 ES 3 5 L-iE i-i3 SE S3 7fcJ
Time, days
Figure B.65. Soluble biological oxygen demand concentration
of influent sewage to the first unit of the
Hanover NFT in Phase 1. First day of analysis
was March 4, 1981.
1 -1 0 ,	
33--
3 3-	/1 13?
/ '	'r
^ j / <3—^
BB-	U
cz ir: . •• S
^
33 f
ES --
•1 -| ...
0 I	t	t	I	t	I	1	1	1	I	
0 r m 3-1 SB 35 1-.3 u,g 55 53 70
Time, days
Figure B.66. Soluble biological oxygen demand concentration
of effluent sewage from the first unit of the
Hanover NFT in Phase 1. First day of analysis
was March 4, 1981.


A
P
V 1
} 1
/ 'i
, .-"V l
0 a 0
/ 't*		


• / "-o-"



367

-------
'1
33 --
BB --
BB-
SS--
s—.
SS ¦¦
0 7
m ai EB 35 1-.E >-,3 SB E3 70
Time, days
Figure B.67. Soluble biological oxygen demand concentration
of effluent sewage from the second unit of the
Hanover NFT in Phase 1. First day of analysis
was March 4, 1981.
"V V
«-.a SB B3

Figure B.68.
Time, days
Soluble biological oxygen demand concentration
of effluent sewage from the third unit of the
Hanover NFT in Phase 1. First day of analysis
was March 4, 1981.
368

-------

35 0
" 3-1 5 c j
EB0
SI-.5--
S-1 0--
17 5-
•1 L-.0 --
•10S--
70-.
35-
0	
0 I
Figure B.69,
A
.a,
^ B
-&ei
V"*

IE E<-I 3 S 1-10 LiB 55 EL, 75 B0
Time, days
Chemical oxygen demand concentration of influent
sewage to the first unit of the Hanover NTT in
Phase 1. First day of analysis was March 4,
1981.
35 8
— —J •-




3-1 5 .




5B0-


ft

S<-.5 .
3-1 0 •
•1 7 5 -
"1 Li 0 .
1.
—	
/ \ .¦#
1 ,' la.
—R ''
- ' c
i3
*—¦—•a
10 5-




70 -




35 -




L~1 .




SB -IE Bi-i 35 '—10 L-iG 5E EL, 72 B0
Time, days
Figure B.70. Chemical oxygen demand concentration of effluent
sewage from the first unit of the Hanover NFT
in Phase 1. First day of analysis was March 4,
1981.
369

-------
350
3-1 5 -¦
EB0--
E>-.5..
E-1 0
'175
-m0
105
70-
35-
0
W

R

.A

V
0 5
1E El-. 3E i-i0	5E EL, 72 B0
Figure B.71.
Time, days
Chemical oxygen demand concentration of
effluent sewage from the second unit in the
Hanover NFT in Phase 1. First day of analysis
was March 4, 1981.
31 5-.
EB0--
EL.5..
E-1 0--
-175--
-m©..
'10 5
70
35
3
0
-vv"\
p
ils.

'ra
V*
1E El-.
3 E ^0 LiB
Time, days
5E EU 7E 50
Figure B.72.
Chemical oxygen demand concentration of
effluent sewage from the third unit of the
Hanover NFT in Phase 1. First day of analysis
was March 4, 1981.
370

-------
E5 0
EEB--
E0E -•
ib>-i
•1 BE
me--
-1 -1 B -.
3E --
55-

Tas—a

J\ A
/' Vq
a a
30
0 B
Figure B.73.
-IE Ei-i 32 Li0 1-.B 5E Eh 7S B0
Time, days
Soluble chemical oxygen demand concentration
of influent sewage to first unit of Hanover
NFT in Phase 1. First day of analysis was
March 4, 1981.
30
Q
/Vk .
\ r
a

/
•1E
3 E l,0 <-.B
Time, days
EE Eh ?E B0
Figure B.74.
Soluble chemical oxygen demand concentration
of effluent sewage from first unit of Hanover
NFT in Phase 1. First day of analysis was
March 4, 1981.
371

-------
E50
EEB--
E0E --
•IB1-!-.
'1EE--
-11-.0
'1 '1 E
BE
71-.
5E +
r—
ivy
I
/ \ D
/ / W" \ .
30
0 B
A E El-. EE 10 LiB EE El-. ?E B0
Figure B.75.
Time, days
Soluble chemical oxygen demand concentration
of effluent sewage from second unit of Hanover
NFT in Phase 1. First day of analysis was
March 4, 1981.
EEB --
E0E--
•1 B1-!
•1 EE
-1 L-.0
•1 -1 B
BE
7h
EE
A,
I
B-
aj


W / \

Time, days
Figure B.76,
Soluble chemical oxygen demand concentration
of effluent sewage from third unit of Hanover
NFT in Phase 1. First day of analysis was
March 4, 1981.
372

-------
A
i \ .5? I \
/ V X I. J. V
0
•m SI SB 35 >-.£	5E E3 70
Time, days
Figure B.77. Total nitrogen concentration of influent
sewage to the first unit of Hanover NFT in
Phase 1. First day of analysis was March 4,
1981.
35 i	
33 --
30 --
P
SB-	} \_
/ 7
as--	& \ cj,ax
'	'¦ / E1
« aa7\ / \	^
f seif v/	V
* IB-
H
1	5--
-1	3--
1	0 	1	I	I	t	I	1	1	I	I	
0 7 m S-1 EB 35 1-.E L-3 5B B3 70
Time, days
Figure B.78. Total nitrogen concentration in effluent
sewage from the first unit of Hanover NFT
in Phase 1. First day of analysis was
March 4, 1981.

r-
v%
~Y

V '
373

-------
35
30 --
SB-.
Q—El
<1 B
-m 2-1 SB 35 i-iB I-.3 5E S3 70
0 7
Time, days
Figure B.79. Total nitrogen concentration of effluent
sewage from second unit of Hanover NFT in
Phase 1. First day of analysis was
March 4, 1981.
33
30
2B
25
23
20
•1 B
1 5
•1 3
-1 0
0 7 -m 2-1 2B 35 Li2 ^3 5E S3 7©
Time, days
Figure B.80. Total nitrogen concentration of effluent
sewage from third unit of Hanover NFT in
Phase 1. First day of analysis was
March 4, 1981.
37U

-------
30
27 --
Si-i
21 .
1B-
'1 5--
'1 5--
3 ¦¦
E -¦
3 ¦¦
¦•f\A
0

A
LA
a
/
0 B
IB S«-i 32 *-» 0 1iB SB B1-! 72 B0
Figure B.81.
Time, days
Ammonium concentration of influent sewage to
first unit in Hanover NTT in Phase 1. First
day of analysis was March 4, 1981.

SJ 1C.I -
27 -


5j>
o*
00
e
2i-i-
2-1 -
•1 B -
[
1 B -
-1 2-
\ &Q-
]

i
V
fif
•£
B ¦



+ ~141
0 B '1B 21-! 32 i-i0 L-tB 5B Ei-i 72 B0
Time, days
Figure B.82. Ammonium concentration of effluent sewage from
first unit in Hanover NFT in Phase 1. First
day of analysis was March 4, 1981.
37 5

-------
30
a?
a »i
a-i
-i a
•1 5
-1 a
3
E
3
0
0 b is a«-i sa >i0 i-iB se EL, ?a B0
Time, days
Figure B.83. Ammonium concentration of effluent sewage from
second unit in Hanover NTT in Phase 1. First
day of analysis was March 4, 1981.
B7
a«-.
ai
'1 B
•1 s
-1 a
3
E
3
0
0 B -IE a1-. 3a 1-10 "-iB 5E EL-. ?a B0
Time, days
Figure B.84. Ammonium concentration of effluent sewage from
third unit in Hanover NFT in Phase 1. First
day of analysis was March 4, 1981.
:P\
V'V-
v
/y i-vi
V
I	I	I	I	I	<	I	I	I

376

-------
0 ma cmd am—a a t t aa—~~ ma ~~ eg—a—
0 B -IE Ei-i 32 l,0 "-iB SE E"h	B0
Figure B.85.
Time, days
Nitrate concentration of influent sewage to
first unit in Hanover NFT in Phase 1. First
day of analysis was March 4, 1981.
1 Q
bJ
1E
EE E Li 73 B0
33 >i0 LiB
Time, days
Figure B.86. Nitrate concentration of effluent sewage from
first unit in Hanover NFT in Phase 1. First
day of analysis was March 4, 1981.
37 T

-------
to
e
z
i
I m
O
z
0 "Pe
99
B-B
3S Li 0 LiB
Time, days
B0
Figure B.87. Nitrate concentration of effluent sewage from
second unit in Hanover NFT in Phase 1. First
day of analysis was March 4, 1981.
oo
a
t O
O
z
¦
0
•1E E*-i
35 h0 1-.B
Time, days
55 Bh 75 B0
Fiyure B.88.
Nitrate concentration of effluent sewage from
third unit in Hanover NFT in Phase 1. First
day of analysis was March 4, 1981.
378

-------
Time, days
Figure B.89. Total phosphorus concentration of influent
sewage to first unit in Hanover NFT in Phase 1.
First day of analysis was March 4, 1981.
—I i	!
Z t*	I
'	i
I .i.	;
¦:;i 4	1	1	1	!	;	;	i	1	!	!
0 ~ 1 S E"-i	SE G '-i r'3 E30
Time, days
Figure B.90. Total phosphorus concentration of effluent
sewage from first unit in Hanover NFT in
Phase 1. First day of analysis was March 4,
1981.
379

-------
¦J
~
B
cr
L-i
3
a
1
0
a a IE a1-. 3 a 1-.0 ^b sb s 7 a 00
Time, days
Figure B.91. Total phosphorus concentration of effluent
sewage from second unit in Hanover NFT in
Phase 1. First day of analysis was March 4
1981.
1 0
3
B
~F
B
5
i-i
3
a
'1
0
Q B IE a^ 3 a 1-10 LiB 5E SI-. 7 3 B0
Time, days
Figure B.92. Total phosphorus concentration of effluent
sewage from third unit in Hanover NFT in
Phase 1. First day of analysis was March 4
1981.
1 ''••• ^
--i
i 'd
.P?
/ \
•3 ...a. 		——^
13
380

-------
•1 0
3
B
7
E
5
3
a
1
0
0 B -1E E1-! 3 a 1-10 hB SE E1-.	B0
Time, days
Figure B.93. Orthophosphate concentration of influent sewage
to first unit in Hanover NFT in Phase 1. First
day of analysis was March 4, 1981.

/V



-t	1	1	t	ttii
M
•IE a^i 3a '—i 0 LiB SE E1-!
Ba
Figure B.94.
Time, days
Orthophosphate concentration of effluent sewage
from first unit in Hanover NFT in Phase 1.
First day of analysis was March 4, 1981.
381

-------
A / '^1
0 B
-IE E"-. 35 >10 '-iB BE E^i	BQ
Figure B.95,
Time, days
Orthophosphate concentration of effluent sewage
from second unit in Hanover NFT in Phase 1.
First day of analysis was March 4, 1981.
A / fcr-"5?
0 B 15 EL-, 3 a !-iQ L-.a 5E EL-i 72 BO
Figure B.96.
Time, days
Orthophosphate concentration of effluent sewage
from third unit in Hanover NFT in Phase 1.
First day of analysis was March 4, 1981.
332

-------
30
B '1
75
E3
Si-1
•-i 5
3E
a?
•1 a
3
0
0 B 'IE S1-! 35 L-i0 LiB EE E^-. 75 B0
Time, days
Figure B.97. Total suspended solids concentration of
influent sewage to first unit in Hanover NFT
in Phase 1. First day of analysis was March
4, 1981.
>—15 - -
3E--
0 B
•1E 5"-. 35 1-.0 1-.B 5E Ei-. 75 B0
Time, days
Figure B.98. Total suspended solids concentration of effluent
sewage from first unit in Hanover NFT in Phase
1. First day of analysis was March 4, 1981.
383

-------
30T-
B-1 --
75-
S3-
B1-! -¦
L-iB -¦
35--
E? ¦¦
•IB-
~ --
0 -I
0
.P-v

t	i


\ A
i	i	i
B -1E El-. 32 i-i0 1-.B SB BI-. ?E B0
Time, days
Figure B.99. Total suspended solids concentration of effluent
sewage from second unit in Hanover NFT in Phase
1. First day of analysis was March 4, 1981.
oo
S
zn
oo
H
32 1-.0 LiB BB Bi-i 72 B0
Figure B.100.
Time, days
Total suspended solids concentration of
effluent sewage from third unit in Hanover
NFT in Phase 1. First day of analysis was
March 4, 1981.
38U

-------
0 B
IE S»i 3 2 0 hB BE Eh 7 2 B0
Time, days
Figure B.101. Volatile suspended solids concentration of
influent sewage to the first unit in Hanover
NFT in Phase 1. First day of analysis was
March 4, 1981.
Time, days
Figure B.102. Volatile suspended solids concentration of
effluent sewage from first unit in Hanover
NFT in Phase 1. First day of analysis was
March 4, 1981.
385

-------
1 !-

3 i'	'i	i
"73--	C	PL.	rk
pj £	d3—-ed -S—"a
JI
at
ij B 1 S 21—i 3 2 L-i 0 1—113 bata E1-! r" 2 Bu
Time, days
Figure B.103. Volatile suspended solids concentration of
effluent sewage from second unit in Hanover
NTT in Phase 1. First day of analysis was
March 4, 1981.
a
1E 2L-.
3 2 '-it) L-iB
Time, days
5E Ei-. 7 2 SQ
Figure B.104. Volatile suspended solids concentration of
effluent sewage from third unit in Hanover
NFT in Phase 1. First day of analysis was
March 4, 1981.
386

-------
0
Figure B.105.
b 1 a -1 a
Time, days
•1 !-i ¦I G -1 B 20
pH of influent sewage to first unit in Hanover
NFT in Phase 1. First day of analysis was
March 2, 1981.
-1 0
id
•10 -1 a 1L-, 1 B 1 B E0
Time, days
Figure B.106. pH of effluent sewage from first unit in
Hanover NFT in Phase 1. First day of analysis
was March 2, 1981.
387

-------
-1 0
Time, days
-1 i-i -1 E -IB SQ
Figure B.107. pH of effluent sewage from second unit in
Hanover NFT in Phase 1. First day of analysis
was March 2, 1981.
Time, days
Figure B.108. pH of effluent sewage from third unit in
Hanover NFT in Phase 1. First day of analysis
was March 2, 1981.
388

-------
Time, days
Figure B.109. Minimum daily air temperature in the Hanover
NFT greenhouse. First day of measurement was
April 2, 1981.
y
0 +
i
3 51
3 ii i-_
a
i3?
i
!*'
<1 .'fo
A I! %T \ !
3
' ij ft $ ^
raj J.Vi na :T.iii -j
a
Q .11 {
¦irt cr-

a
¦ 11 i i
•;r~ 2
-- T ,*•,
Time, days
Figure B.110. Maximum daily air temperature in the Hanover
NFT greenhouse. First day of measurement was
April 2, 1981.
389

-------

§
EC
(1)
>
•H

I ii
I l-!3
i' 'i
Q
3 icj ^ A
i H U
I V
:il '5 I
i 2?
•'! 0 50
Figure B.lll.
Time, days
a ¦;'! -100
Minimum daily relative humidity in the Hanover
NFT greenhouse. First day of measurement was
March 2, 1981.
'1 0 0
A
>.
4J
•H
13


-------
25 0
5-1 5--
-55-
—100
S -15 30 «-<5 B0 75 30 -1 05 -150 135 -150
TIME, days
Figure B.113. "Evapotranspiration from the Hanover three
unit NFT system in Phase 1. First day of
measurement was February 26, 1981.
391

-------
APPENDIX C
This appendix presents data developed at the Cornell University facilities
over the course of the project. Tables C.l through C.25 present mean values
of variables over each loading condition tested. The complete data set is
presented in three chronological sets. The first set (Figures C.l to C.98)
2
showB data from the 9.3 m system that was sampled at the influent and
effluent stations, as well as intermediate points (noted as points A and B,
which were about one-third and two-thirds of the distance through the
system, respectively). The second set (Figures C.99 to C.201) shows later
2
experiments using both 9.3 and 18.6 m systems, which were sampled at points
2
identical to those of earlier experiments. In the 18.6 m system the
effluent of the first section was the influent to the second section; thus
no influent data is shown for the second. The third set (Figures C.202 to
2
C.315) shows experiments in the 27.9 m system, which was made up of three
9.3 m sections connected in series. No intermediate point sampling data
was conducted on this large unit. A fuller discussion of test units and
loading conditions is presented in Chapter 6, Section A.2.b.
392

-------
TABLE C.l. LOADING CONDITION PARAMETERS TESTED IN PILOT SCALE NFT SYSTEMS AT
CORNELL USING SYNTHETIC WASTEWATER. CONDITION NUMBERS ARE GUIDES
TO SPECIFIC VARIABLE DATA WHICH FOLLOWS.
Target Loading
	Rate	
Area Weir
Condi- Loading Loading
tion Rate Rate
No. cm/d m /m-d
Condition
Dates
Start
End
System
Area
m
Plant
Species
Application
Mode
1
10.2
1.24
12/ 6/79
1/ 2/80
9.3
Canary
Grass
Continuous
2
10.2
1.24
1/ 3/80
1/27/80
9.3
Canary
Grass
30 min on
30 min pause
3
10.2
1.24
1/28/80
2/20/80
9.3
Canary
Grass
30 min on
105 min pause
4
10.2
1.24
2/21/80
3/17/80
9.3
Canary
Grass
30 min on
90 min pause
5
5.1
0.62
3/18/80
5/11/80
9.3
Canary
Grass
Continuous
6
10.2
1.24
5/12/80
6/17/80
9.3
Canary
Grass
Continuous
7
5.1
0.62
6/18/80
7/ 3/80
9.3
Canary
Grass
Continuous
8
20.3
2.49
7/ 4/80
9/ 8/80
9.3
Canary
Grass
Continuous
9
10.2
2.49
9/ 9/80
10/15/80
18.6
Canary
Grass
Continuous
10
20.3
4.98
10/16/80
10/31/80
18.6
Canary
Grass
Continuous
11
6.9
2.49
2/13/81
3/14/81
27.9
Phragmites*
Continuous
12
13
14
15
16
10.2
20.3
40.6
6.9
10.2
3.74 4/16/81 5/10/81 27.9
7.47 5/11/81 6/10/81 27.9
14.94 6/11/81 7/ 1/81 27.9
2.49
7/ 2/81 7/30/81 27.9
3.74 7/31/81 8/21/81 27.9
Cucumbert
Canary Grass§
Cattail/Bulrush* Continuous
Phragmitest
Canary Grass§
Cattail/Bulrush* 30 min on
Phragmitest 30 min pause
Canary Grass§
Cattail/Bulrush* 30 min on
Phragmitest 30 min pause
Canary Grass§
Cattail/Bulrush* Continuous
Phragmitest
Canary Grass§
Cattail/Bulrush* 30 min on
Phragmitest 30 rain pause
Canary Grass§
*Unit 1, tUnit 2, §Unit 3.
393

-------
TABLE C.2. TOTAL CHEMICAL OXYGEN DEMAND CONCENTRATIONS (mg/fc) AT VARIOUS POINTS IN THE CORNELL
BRACEHOUSE PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN
PARENTHESES.

Actual





Mass
No. of



Condi-
Loading
Deter-



tion
Rate
mina- Unit 1
Unit
2
Unit 3
Number
kg/ha-d
tions Influent Point A Point B Effluent
Point A Point
B Effluent
Effluent
1
412
3
405.0
(16.5)
320.7
(50.8)
235.3
(31.0)
164.7
(24.0)
2
318
3
313.0
(217.6)
233.0
(113.8)
189.0
(59.1)
165.0
(18.3)
3
377
4
371.0
(53.9)
323.2
(23.4)
302.0
(50.0)
269.0
(29.5)
4
407
4
400.2
(40.7)
297.2
(45.4)
237.5
(92.4)
147.2
(26.3)
5
215
8
423.4
(41.7)
256.4
(37.2)
138.4
(29.3)
55.0
(22.6)
6
280
5
275.6
(18.6)
161.8
(19.4)
118.0
(31.2)
38.4
(15.7)
7
133
3
260.7
(64.0)
108.0
(32.1)
77.0
(9.6)
62.3
(16.4)
8
392
5
192.8
(23.3)
134.4
(10.7)
83.0
(31.3)
62.4
(16.8)
table continued.

-------
TABLE C.2. continued
Actual
Mass	No. of
Condi- Loading	Detec-
tion Rate	raina- 	Unit 1	 	Unit 2	 Unit 3
Number kg/ha-d	tions Influent Point A Point B Effluent Point A Point B Effluent Effluent
9
210
3
207.7
(26.0)
146.3
(21.7)
147.0
(44.8)
31.2
(12.0)
62.7
(2.5)
60.7
(3.4)
19.7
(8.0)

10
395
4
194
(28)
158
(36)
140
(48)
81
(38)
97
(39)
72
(35)
26
(20)

11
187
6
275.0
(84.7)
—
—
156.7
(43.8)
—
—
103.5
(37.3)
48.3
(48.6)
12
185
4
181.5
(25.8)
—
—
100.00
(29.7)
—
—
65.2
(26.7)
46.8
(11.8)
13
348
5
170.8
(41.0)
—
—
125.8
(67.6)
—
—
85.6
(37.5)
31.6
(19.3)
14
972
3
238.7
(50.0)
—
—
242.0
(67.2)
—
—
215.0
(42.6)
137.0
(15.4)
15
144
2
212.5
(67.2)
—
—
143.0
(21.2)
—
—
401.3
(541.0)
144.0
(136.2)
16
460
4
452.2
(115.0)
—
—
306.7
(90.3)
—
—
173.3
(97.5)
127.0
(68.6)

-------
TABLE C.3. SOLUBLE CHEMICAL OXYGEN DEMAND CONCENTRATIONS (mg/l) AT VARIOUS POINTS IN THE CORNELL
BRACEHOUSE PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN
PARENTHESES.
Condi-
tion
Actual
Mass
Loading
Rate
No. of
Deter-
mina-

Unit
1
Unit 2 Unit 3
Number
kg/ha-d
tions
Influent
Point A
Point B
Effluent Point A Point B Effluent Effluent
1
412
3
405.0
(18.5)
250.0
(48.7)
222.3
(26.7)
163.7
(34.8)
2
382
3
375.7
(90.8)
208.0
(127.7)
173.3
(61.2)
175.7
(22.3)
3
392
4
385.8
(20.7)
324.2
(25.2)
280.2
(48.6)
258.5
(39.7)
4
382
4
375.8
(24.0)
252.8
(21.7)
158.2
(18.0)
145.5
(26.3)
5
213
8
419.5
(61.5)
217.9
(44.8)
112.4
(26.7)
62.5
(19.4)
6
278
5
273.4
(29.5)
134.4
(13.6)
67.8
(6.6)
33.4
(6.9)
7
128
3
252.3
(59.0)
103.3
(21.2)
53.0
(9.2)
50.0
(24.6)
8
376
5
184.8
(18.5)
110.8
(11.6)
73.0
(18.5)
62.0
(24.6)
table continued.

-------
TABLE C.3. continued

Actual




Mass
No. of


Condi-
Loading
Deter-


tion
Rate
mina-
Unit 1 Unit 2
Unit 3
Number
kg/ha-d
tions
Influent Point A Point B Effluent Point A Point B Effluent
Effluent
9
101
3
99.3
131.7
82.0
50.7
35.3
23.7
13.7




(23.1)
(22.9)
(23.6)
(17.7)
(12.7)
(7.1)
(2.1)

10
391
4
192
143
94
72
51
33
26




(24)
(26)
(30)
(27)
(27)
(29)
(21)

11
194
6
286.2
—
—
174.2
—
—
106.8
36.3



(96.3)
—
—
(106.9)
—
—
(51.1)
(28.1)
12
173
4
170.2
—
—
91.0
—
—
60.8
41.8



(51.4)
—
—
(52.1)
—
—
(36.5)
(34.3)
13
375
5
184.4
—
—
85.2
—
—
83.6
32.0



(56.0)
—
—
(49.1)
—
—
(42.4)
(19.2)
14
903
3
222.0
	
—
246.3
—
—
178.7
108.7



(37.0)
—
—
(67.9)
—
—
(53.7)
(17.9)
15
151
2
222.5
—
—
169.7
—
—
89.3
74.0



(50.2)
—
—
(88.7)
—
—
(48.8)
(35.1)
16
390
3
383.7
—
—
272.3
—
—
160.7
78.3



(122.7)
—
—
(142.2)
—
—
(103.8)
(70.0)

-------
TABLE C.4. TOTAL NITROGEN CONCENTRATIONS (mg/£) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT
SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.

Actual





Mass
No. of



Condi-
Loading
Deter-



tion
Rate
mina-
Unit 1
Unit 2
Unit 3
Number
kg/ha-d
tions
Influent Point A Point B Effluent
Point A Point B Effluent
Effluent
1
38.7
3
38.0
36.5
35.2
31.4



(4.4)
(3.1)
(1.6)
(4.1)
2
42.2
3
41.5
33.6
31.6
29.2



(3.4)
(8.0)
(6.4)
(5.0)
3
34.2
4
33.6
32.5
32.1
33.9



(5.2)
(6.1)
(5.5)
(5.8)
4
36.3
2
35.7
30.3
28.9
26.2



(3.4)
(12.3)
(5.6)
(2.7)
5
19.0
6
37.4
36.5
31.6
29.2



(4.1)
(3.0)
(6.4)
(4.1)
6
41.9
6
41.2
38.6
35.6
31.8



(3.3)
(4.3)
(3.7)
(3.8)
7
18.8
2
37.0
29.0
21.4
25.2



(1.6)
(6.9)
(12.0)
(0.0)
8
71.1
5
35.0
32.4
30.0
27.3



(4.9)
(4.8)
(3.7)
(3.4)
table continued...

-------
TABLE C.4. continued

Actual





Mass
No. of



Condi-
Loading
Deter-



tion
Rate
mina-
Unit 1 Unit
2
Unit 3
Number
kg/ha-d
tions
Influent Point A Point B Effluent Point A Point
B Effluent
Effluent
9
34.1
3
33.6
(8.2)
27.3
(6.6)
26.7
(4.4)
24.1
(3.5)
26.1
(1.2)
27.0
(4.1)
14.4
(9.4)

10
35.8
4
17.6
(8.8)
33.6
(3.9)
33.8
(4.9)
30.5
(5.0)
31.0
(4.4)
29.8
(2.4)
30.4
(2.8)

11
26.5
6
39.1
(3.0)
—
—
34.2
(3.2)
—
—
32.0
(2.1)
30.4
(2.1)
12
39.7
5
39.0
(6.1)
—
—
35.1
(5.1)
—
—
33.4
(5.9)
32.5
(5.9)
13
36.2
6
17.8
(4.0)
—
—
19.3
(9.3)
—
—
11.7
(2.6)
11.6
(3.7)
14
140.8
4
34.6
(3.3)
—
—
35.2
(3.7)
—
—
35.2
(5.6)
32.6
(3.7)
15
13.3
3
19.6
(11.6)
—
—
23.3
(10.1)
—
—
26.9
(10.2)
22.3
(11.2)
16
57.1
3
56.1
(4.6)
—
—
55.3
(10.1)
—
—
52.0
(15.3)
51.4
(8.3)

-------
TABLE C.5. SOLUBLE TOTAL NITROGEN CONCENTRATIONS (mg/fc) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE
PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.

Actual





Mass
No. of



Condi-
Loading
Deter-



tion
Rate
mina-
Unit 1 Unit
2
Unit 3
Number
kg/ha-d
tions
Influent Point A Point B Effluent Point A Point
B Effluent
Effluent
1
38.8
2
38.1
33.8
34.0
28.5



(4.9)
(4.4)
(1.5)
(3.2)
2
38.4
3
37.7
33.3
30.8
28.8



(2.2)
(7.0)
(4.0)
(4.4)
3
37.3
4
36.6
33.5
30.4
30.1



(4.3)
(6.1)
(5.5)
(4.6)
4
38.6
3
37.9
26.8
25.5
26.0



(3.9)
(7.9)
(0.4)
(2.8)
5
19.3
6
37.9
33.6
31.9
28.8



(5.9)
(6.7)
(7.1)
(3.7)
6
41.4
6
40.7
34.8
34.1
32.8



(3.2)
(4.0)
(5.0)
(5.9)
7
18.3
2
36.0
29.9
28.8
21.6



(0.6)
(4.2)
(0.0)
(0.0)
8
68.5
5
33.7
31.6
28.7
27.2



(4.4)
(4.9)
(3.7)
(3.1)
table continued.

-------
TABLE C.5. continued
Condi-
tion
Number
Actual
Mass
Loading
Rate
kg/ha-d
No. of
Deter-
mina-
tions
Unit 1
Unit 2
Influent Point A Point B Effluent Point A Point B Effluent
Unit 3
Effluent
9
37.0
3
36.4
(12.4)
28.4
(6.0)
25.7
(4.1)
22.8
(2.1)
26.8
(4.0)
26.4
(4.2)
23.8
(2.9)

10
38.3
4
18.8
(7.7)
28.3
(2.6)
29.0
(4.0)
30.3
(4.2)
34.1
(5.1)
29.9
(2.8)
30.8
(0.8)

11
25.3
6
37.3
(6.0)
—
—
32.5
(3.4)
—
—
30.6
(2.1)
29.0
(2.1)
12
37.6
5
37.0
(5.3)
—
—
30.7
(3.8)
—
—
29.7
(4.9)
31.1
(5.6)
13
34.8
6
17.1
(3.6)
—
—
13.6
(4.4)
—
—
10.3
(2.2)
10.8
(4.0)
14
132.3
4
32.5
(1.6)
—
—
35.8
(5.3)
—
—
32.8
(4.1)
32.0
(3.6)
15
11.3
—
16.6
(11.1)
—
—
19.5
(9.3)
—
—
19.5
(8.3)
21.1
(8.4)
16
58.6
—
57.6
(5.0)
—
—
50.3
(8.9)
—
—
50.9
(8.2)
49.9
(8.4)

-------
TABLE C.6. ORGANIC NITROGEN CONCENTRATIONS (mg/£) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE
PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
1
3
34.6
12.5
5.8
4.3


(3.8)
(1.1)
(0.6)
(1.1)
2
3
38.1
23.2
10.0
5.3


(3.3)
(3.9)
(2.5)
(0.6)
3
3
26.1
24.8
23.6
25.6


(3.7)
(10.0)
(8.1)
(9.2)
4
2
32.9
19.7
10.1
6.7


(1.8)
(6.8)
(5.3)
(1.4)
5
6
30.6
8.9
5.0
5.1


(6.0)
(2.5)
(3.0)
(2.4)
6
6
33.6
7.0
3.7
3.7


(5.7)
(2.7)
(2.3)
(3.7)
7
2
33.3
1.6
0.0
1.1


(0.4)
(2.6)
(0.0)
(0.0)
8
5
29.9
5.1
2.7
2.1


(5.8)
(2.2)
(1.0)
(0.7)
table continued..

-------
TABLE C.6. continued
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
9	3
10	4
11	4
12	5
13	6
14	4
15	3
16	3
16.3
(1.8)
1.0
(1.0)
16.9
(9.1)
8.7
(2.5)
34.3
(8.4)
—
36.6
(7.3)
—
16.9
(3.9)
—
31.1
(3.8)
—
7.9
(5.4)
—
46.7
(3.4)
—
0.7
0.3
(0.1)
(0.5)
6.0
1.3
(2.4)
(1.5)
—
3.2
—
(0.5)
—
11.7
—
(1.3)
—
13.8
—
(9.8)
—
18.0
—
(2.1)
—
3.6
—
(3.1)
—
4.9
—
(1.6)
2.6	2.7
(1.3)	(1.3)
1.9	2.8
(2.0)	(1.9)
2.3
(1.9)

1.9
(2.5)

1.8
(0.6)
1.0
(0.7)
6.6
(1.7)
2.7
(1.2)
4.2
(2.3)
3.0
(3.7)
8.7
(3.3)
3.8
(1.1)
4.8
(2.8)
1.6
(2.3)
3.2
(1.3)
1.5
(0.6)

-------
TABLE C.7. SOLUBLE ORGANIC NITROGEN CONCENTRATIONS (mg/£) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE
PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.

Condition
Number
of Deter-
Unit 1

Unit
2
Unit 3
Number
minations
Influent Point A Point B
Effluent
Point A Point
B Effluent
Effluent
1
2
35.4
9.7
4.6
2.2


(3.9)
(3.0)
(0.2)
(0.3)
2
3
35.3
17.6
9.0
4.1


(2.7)
(7.1)
(5.1)
(1.0)
3
3
29.6
23.0
22.7
24.1


(7.4)
(10.1)
(8.1)
(4.1)
4
2
37.7
9.6
8.1
4.9


(3.0)
(7.6)
(3.5)
(3.4)
5
6
29.5
5.1
4.1
5.0


(6.0)
(5.3)
(4.5)
(4.3)
6
6
33.7
3.1
2.5
4.5


(6.1)
(2.0)
(2.1)
(5.4)
7
2
33.4
2.1
1.1
0.6


(1.3)
(0.7)
(0.0)
(0.0)
8
5
28.3
4.8
1.8
1.5


(6.0)
(2.7)
(0.7)
(0.6)
table continued.

-------
TABLE C.7. continued
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number rainations Influent Point A Point B Effluent Point A Point B Effluent Effluent
9
3
16.4
(1.9)
1.7
(0.3)
0.1
(0.1)
0.2
(0.3)
2.1
(1.4)
2.1
(2.1)
1.6
(1.3)

10
4
18.3
(8.0)
4.2
(1.7)
1.3
(1.5)
1.4
(1.6)
3.9
(5.0)
2.1
(2.4)
1.1
(2.3)

11
—
32.1
(8.6)
—
—
3.0
(1.0)
—
—
1.1
(0.6)
0.6
(0.4)
12
4
33.1
(8.2)
—
—
10.1
(3.2)
—
—
3.3
(1.1)
1.7
(0.7)
13
5
15.9
(3.9)
—
—
8.0
(3.2)
—
—
2.9
(1.9)
3.6
(6.8)
14
6
28.0
(3.3)
—
—
11.3
(4.0)
—
—
5.5
(2.7)
1.9
(1.1)
15
4
5.0
(2.2)
—
—
0.8
(0.6)
—
—
1.0
(1.1)
0.8
(0.9)
16
—
49.4
(6.6)
—
—
2.9
(1.7)
—
—
2.4
(1.2)
2.1
(0.6)

-------
TABLE C.8. AMMONIUM NITROGEN CONCENTRATIONS (mg/£) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE
PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.

Condition
Number
of Deter-

Unit 1
Unit
2
Unit 3
Number
minations
Influent
Point A Point B Effluent
Point A Point
B Effluent
Effluent
1
2
1.2
23.0
28.7
25.6


(1.7)
(3.2)
(0.0)
(3.3)
2
3
0.9
9.7
21.5
23.7


(0.9)
(10.8)
(7.7)
(4.9)
3
3
3.7
6.7
8.2
7.4


(1.3)
(3.9)
(4.1)
(4.7)
4
2
0.7
11.6
17.7
18.9


(1.0)
(10.2)
(3.2)
(1.1)
5
8
8.1
26.2
24.9
22.9


(7.8)
(4.6)
(6.0)
(5.0)
6
6
7.6
31.6
32.0
28.0


(6.4)
(2.7)
(1.9)
(3.2)
7
3
2.4
20.7
17.2
12.0


(2.3)
(12.0)
(10.5)
(12.0)
8
5
5.1
27.3
27.3
25.2


(1.8)
(3.8)
(3.6)
(3.2)
table continued.

-------
TABLE C.8. continued
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
9
3
13.1
(11.5)
29.9
(3.2)
31.0
(4.7)
27.5
(5.2)
32.4
(7.6)
26.5
(3.6)
23.0
(4.3)

10
4
0.6
(1.3)
24.9
(2.5)
27.7
(3.0)
29.2
(3.5)
28.9
(4.2)
27.0
(2.6)
28.5
(3.6)

11
6
4.0
(5.5)
—
—
31.0
(3.0)
—
—
29.8
(2.6)
29.2
(2.5)
12
5
2.4
(1.4)
—
—
23.4
(5.5)
—
—
26.8
(4.9)
29.8
(4.7)
13
6
0.9
(1.0)
—
—
5.2
(2.8)
—
—
7.4
(2.7)
8.7
(2.3)
14
4
3.5
(1.8)
—
—
21.2
(3.4)
—
—
28.4
(3.0)
31.3
(3.3)
15
4
15.0
(10.8)
—
—
21.4
(8.0)
—
—
22.7
(6.3)
21.5
(7.5)
16
4
7.7
(6.6)
—
—
47.8
(9.9)
—
—
47.0
(8.9)
46.5
(9.2)

-------
TABLE C.9. SOLUBLE AMMONIUM NITROGEN CONCENTRATIONS (mg/£) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE
PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.


Number






Condition
of Deter-

Unit 1

Unit
2
Unit 3
Number
minations
Influent
Point A Point B
Effluent
Point A Point
B Effluent
Effluent
1
1
0.4
23.5
28.1
24.2


(0.0)
(1.4)
(0.0)
(0.0)
2
3
0.6
14.3
23.0
24.6


(0.6)
(13.9)
(9.9)
(4.7)
3
3
3.7
10.5
7.1
5.4


(3.7)
(6.2)
(4.1)
(2.4)
4
2
0.6
18.3
17.1
20.5


(0.8)
(7.1)
(3.5)
(0.9)
5
8
9.8
27.4
26.7
23.1


(10.5)
(4.1)
(5.0)
(5.7)
6
6
7.0
32.6
31.6
27.6


(5.5)
(4.1)
(3.4)
(3.7)
7
3
1.7
20.9
22.1
11.9


(1.6)
(12.3)
(7.9)
(10.8)
8
4
5.4
26.6
27.1
25.6


(1.9)
(4.0)
(3.2)
(3.4)
table continued.

-------
TABLE C.9. continued
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number rainatlons Influent Point A Point B Effluent Point A Point B Effluent Effluent
9
3
13.3
(11.8)
29.9
(3.3)
30.4
(3.7)
26.5
(6.2)
32.7
(5.8)
27.2
(3.1)
23.9
(2.3)

10
4
0.4
(0.9)
24.1
(1.0)
27.7
(2.6)
28.9
(2.8)
30.2
(2.0)
26.7
(1.2)
28.7
(2.7)

11
6
3.6
(5.1)
—
—
29.9
(2.8)
—
—
29.5
(2.2)
28.3
(2.2)
12
5
3.9
(4.5)
—
—
20.6
(2.8)
—
—
26.5
(4.6)
29.3
(5.1)
13
6
1.1
(0.9)
—
—
55.8
(1.7)
—
—
7.4
(2.7)
8.7
(2.3)
14
4
3.4
(2.0)
—
—
20.3
(3.8)
—
—
27.3
(1.8)
30.2
(4.0)
15
4
14.4
(9.5)
—
—
19.9
(8.0)
—
—
19.3
(6.7)
20.7
(6.3)
16
4
7.1
(8.3)
—
—
45.2
(8.2)
—
—
46.7
(6.9)
44.7
(8.9)

-------
TABLE C.10. TOTAL KJELDAHL NITROGEN CONCENTRATIONS (mg/£) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE
PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.

Condition
Number
of Deter-

Unit 1
Unit
2
Unit 3
Number
minations
Influent
Point A Point B Effluent
Point A Point
B Effluent
Effluent
1
3
35.9
36.
35.1
31.4


(4.0)
(3.2)
(1.6)
(4.2)
2
3
39.1
32.9
31.4
29.1


(3.0)
(8.0)
(6.2)
(5.0)
3
4
32.5
32.7
32.1
33.0


(6.5)
(6.3)
(4.7)
(5.0)
4
2
33.6
31.3
27.8
25.6


(2.8)
(1.1)
(4.5)
(2.3)
5
6
37.4
36.5
31.6
29.2


(4.1)
(3.0)
(6.4)
(4.1)
6
6
41.2
38.6
35.6
31.8


(3.3)
(4.3)
(3.7)
(3.8)
7
2
37.0
29.0
21.4
25.2


(1.6)
(6.9)
(12.0)
—
8
5
35.0
32.4
30.0
27.3


(4.9)
(4.8)
(3.7)
(3.4)
table continued.

-------
TABLE C.10. continued

Condition
Number
of Deter-

Unit 1


Unit 2

Unit 3
Number
minations
Influent
Point A Point B
Effluent
Point A
Point B
Effluent
Effluent
9
3
16.3
(1.8)
1.0 0.7
(1.0) (0.1)
0.4
(0.5)
2.1
(0.7)
2.1
(1.3)
1.5
(1.9)
	
10
—







11
5
38.6
(3.1)
—
33.8
(3.4)
—
—
32.3
(2.1)
30.2
(2.3)
12
5
36.6
(7.3)
—
11.7
(1.3)
—
—
6.6
(1.7)
2.7
(1.2)
13
6
16.9
(3.9)
—
13.8
(9.8)
—
—
4.2
(2.3)
3.0
(3.7)
14
4
34.6
(3.3)
—
39.3
(5.4)
—
—
37.0
(4.1)
35.1
(3.6)
15
3
19.6
(11.5)
—
23.3
(10.1)
—
—
26.9
(10.2)
22.3
(1.1)
16
3
56.1
(4.6)
—
55.3
(10.1)
—
—
52.0
(15.3)
51.4
(8.3)

-------
TABLE C.ll. SOLUBLE TOTAL KJELDAHL NITROGEN CONCENTRATIONS (mg/A) AT VARIOUS POINTS IN THE CORNELL
BRACEHOUSE PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN
PARENTHESES.
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
1
2
36.1
33.5
33.7
28.4


(4.4)
(4.2)
(1.2)
(3.2)
2
3
35.9
31.8
30.4
28.7


(2.3)
(6.9)
(4.4)
(4.4)
3
4
35.1
34.0
29.8
29.5


(4.7)
(5.0)
(6.2)
(4.9)
4
2
38.2
27.8
23.3
25.4


(2.2)
(6.4)
(3.2)
(2.5)
5
6
37.9
33.6
31.9
28.8


(5.9)
(6.7)
(7.2)
(3.7)
6
6
40.7
34.8
34.1
32.8


(3.2)
(4.0)
(5.0)
(5.9)
7
2
36.0
29.8
28.8
21.6


(0.6)
(4.2)
—
—
8
5
33.7
31.6
28.7
27.2


(5.2)
(4.9)
(3.7)
(3.1)
table continued...

-------
TABLE C.ll. continued

Condition
Number
of Deter-

Unit 1


Unit 2

Unit 3
Number
minations
Influent
Point A Point B
Effluent
Point A
Point B
Effluent
Effluent
9
3
16.4
(1.9)
1.7 0.1
(0.3) (0.2)
0.2
(0.4)
1.8
(1.2)
1.8
(1.6)
1.0
(1.3)
—
10
—







11
5
36.3
(6.1)
— —
33.1
(3.6)
—
—
30.7
(2.2)
29.1
(2.4)
12
5
33.1
(8.2)
—
10.1
(3.2)
—
—
3.3
(1.1)
1.7
(0.7)
13
6
15.9
(3.9)
—
8.0
(3.2)
—
—
2.9
(1.9)
3.6
(6.8)
14
4
32.5
(1.6)
—
35.9
(5.3)
—
—
32.8
(4.1)
32.1
(3.6)
15
4
19.4
(10.6)
—
20.8
(8.0)
—
—
20.3
(7.0)
21.5
(6.9)
16
3
57.6
(5.0)
—
50.3
(8.9)
—
—
50.6
(8.7)
49.9
(8.3)

-------
TABLE C.12. NITRATE NITROGEN CONCENTRATIONS (mg/A) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT
SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
1
—
2.1
0.3
0.0
0.0


(0.6)
(0.3)
(0.0)
(0.1)
2
—
2.1
0.7
0.1
0.1


(0.8)
(0.2)
(0.2)
(0.2)
3
—
1.5
1.3
0.7
1.3


(1.5)
(1.6)
(0.7)
(1.0)
4
—
2.3
1.1
0.7
0.6


(0.6)
(0.9)
(0.6)
(0.5)
5
—
2.5
0.0
0.0
0.0


(0.9)
(0.0)
(0.0)
(0.0)
6
4
3.7
0.0
0.0
0.0


(2.1)
(0.0)
(0.0)
(0.0)

-------
TABLE C.13. SOLUBLE NITRATE NITROGEN CONCENTRATIONS (mf;/*) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE
PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
1
—
2.3
0.5
0.1
0.0


(0.6)
(0.4)
(0.2)
(0.0)
2
—
1.8
1.4
0.3
0.1


(0.2)
(0.3)
(0.5)
(0.2)
3
—
2.0
0.4
1.1
0.6


(2.1)
(0.6)
(1.0)
(1.0)
4
—
2.0
2.7
0.7
0.7


(0.3)
(2.9)
(0.8)
(0.6)
5
—
2.6
0.0
0.0
0.0


(1.2)
(0.0)
(0.0)
(0.0)
6
4
3.4
0.0
0.0
0.0


(1.8)
(0.0)
(0.0)
(0.0)

-------
TABLE C.14.
TOTAL PHOSPHORUS CONCENTRATIONS (mg/Jl) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT
SYSTEMS USINC SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Condi-
tion
Number
Actual
Mass
Loading
Rate
kg/ha-d
No. of
Deter-
mina-
tions
Unit 1
Unit 2
Influent Point A Point B Effluent Point A Point B Effluent
Unit 3
Effluent
1
17.7
2
17.4
16.4
17.2
16.3



(0.9)
(0.8)
(0.6)
(1.6)
2
13.5
3
13.3
8.0
9.2
9.5



(0.9)
(2.4)
(2.2)
(2.5)
3
16.2
4
15.9
16.0
14.7
14.8



(0.3)
(0.9)
(1.4)
(0.5)
4
13.7
3
13.5
13.0
11.0
12.1



(1.2)
(1.7)
(1.4)
(1.5)
5
7.2
8
14.2
14.2
13.2
13.3



(1.8)
(1.7)
(2.1)
(2.8)
6
14.1
6
13.9
14.4
13.5
13.9



(1.8)
(1.6)
(1.8)
(3.0)
7
7.2
3
14.1
10.8
9.5
6.6



(1.1)
(3.7)
(6.4)
(6.1)
8
29.5
5
14.5
14.7
14.9
14.8



(2.4)
(2.3)
(2.8)
(2.4)
table continued.

-------
TABLE C.14. continued
Actual
Mass	No. of
Condi- Loading	Detec-
tion Rate	mina-
Number kg/ha-d	tions
Unit 1
Unit 2
Influent Point A Point B Effluent Point A Point B Effluent
Unit 3
Effluent
9
13.5
3
13.3
(3.0)
11.6
(1.3)
11.6
(1.4)
12.9
(3.0)
11.0
(3.0)
10.0
(4.0)
10.1
(4.6)

10
27.7
4
13.6
(1.3)
12.8
(1.6)
12.7
(2.0)
13.5
(1.8)
12.3
(2.2)
13.8
(1.8)
13.1
(2.4)

11
10.7
6
15.8
(3.7)
—
—
15.6
(3.2)
—
—
14.6
(2.9)
15.4
(3.8)
12
12.3
5
12.1
(2.8)
—
—
11.8
(3.4)
—
—
11.3
(3.4)
11.2
(2.6)
13
12.2
5
6.0
(1.5)
—
—
5.6
(1.6)
—
—
4.9
(1.5)
5.4
(1.7)
14
45.6
4
11.2
(5.2)
—
—
12.6
(2.8)
—
—
12.7
(1.1)
12.7
(3.1)
15
5.7
4
8.4
(4.8)
—
—
7.6
(2.7)
—
—
8.2
(3.2)
8.9
(2.3)
16
—
3
14.7
(3.0)
—
—
15.0
(1.7)
—
—
18.2
(2.6)
15.5
(4.6)

-------
TABLE C.15. SOLUBLE PHOSPHORUS CONCENTRATIONS (mg/A) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT
SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.

Actual




Mass
No. of


Condi-
Loading
Deter-


tion
Rate
mina-
Unit 1 Unit 2
Unit 3
Number
kg/ha-d
tions
Influent Point A Point B Effluent Point A Point B Effluent
Effluent
1
16.9
2
16.6
16.6
15.4
16.4



(2.1)
(0.4)
(2.8)
(1.7)
2
10.9
3
10.7
10.5
9.3
9.5



(4.9)
(2.8)
(2.1)
(1.4)
3
16.0
4
15.7
14.8
14.9
14.5



(1.9)
(0.9)
(1.0)
(0.7)
4
14.3
3
14.1
12.6
13.1
11.9



(0.3)
(0.9)
(2.3)
(2.3)
5
7.2
8
14.2
14.3
13.7
12.8



(1.6)
(1.6)
(2.7)
(2.6)
6
15.1
6
14.8
15.8
14.1
13.6



(1.1)
(2.3)
(1.5)
(2.0)
7
7.4
3
14.5
11.5
8.4
7.0



(1.7)
(3.9)
(5.2)
(6.3)
8
29.3
5
14.4
14.5
15.0
15.0



(3.1)
(2.3)
(2.6)
(2.2)
table continued.

-------
TABLE C.15. continued
Actual
Mass	No. of
Condi- Loading	Deter-
tlon Rate	mlna-
Number kg/ha-d	tlons
Unit 1
Unit 2
Influent Point A Point B Effluent Point A Point B Effluent
Unit 3
Effluent
9
9.9
3
9.7
(2.9)
12.8
(0.7)
12.7
(3.0)
12.5
(3.6)
10.6
(2.1)
10.8
(4.8)
10.2
(5.1)

10
27.1
4
13.3
(1.7)
12.4
(1.2)
14.1
(3.4)
14.1
(2.0)
12.1
(1.8)
13.2
(1.4)
13.2
(2.5)

11
11.3
6
16.6
(3.4)
—
—
16.0
(3.1)
—
—
15.0
(2.7)
15.2
(4.1)
12
13.4
5
13.2
(2.5)
—
—
11.3
(2.8)
—
—
11.5
(4.4)
10.9
(4.2)
13
12.0
-
5.9
(1.3)
—
—
5.6
(0.8)
—
—
4.9
(1.4)
5.7
(1.5)
14
50.1
4
12.3
(6.1)
—
—
13.3
(2.9)
—
—
12.9
(4.5)
12.8
(1.9)
15
6.2
4
9.2
(3.4)
—
—
8.4
(3.3)
—
—
9.9
(2.5)
9.8
(2.8)
16
16.9
3
16.6
(4.3)
—
—
17.4
(0.6)
—
—
16.2
(5.4)
16.5
(2.0)

-------
TABLE C.16. pH AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT SYSTEMS USING SYNTHETIC WASTEWATER.
STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.

Condition
Number
of Deter-

Unit 1

Unit
2
Unit 3
Number
minations
Influent
Point A Point B
Effluent
Point A Point
B Effluent
Effluent
1
3
6.3
6.6
6.8
6.9


(0.2)
(0.0)
(0.2)
(0.1)
2
3
6.6
6.6
6.7
6.9


(0.4)
(0.3)
(0.2)
(0.2)
3
4
6.7
6.8
6.9
7.0


(0.1)
(0.0)
(0.1)
(0.2)
4
4
6.5
6.5
6.6
6.9


(0.2)
(0.2)
(0.3)
(0.2)
5
6
6.3
6.5
6.8
7.1


(0.2)
(0.1)
(0.2)
(0.1)
6
5
6.8
7.0
7.2
7.4


(0.1)
(0.1)
(0.1)
(0.1)
7
3
7.1
7.1
7.3
7.4


(0.3)
(0.2)
(0.1)
(0.2)
8
6
6.9
7.0
7.1
7.2


(0.1)
(0.2)
(0.1)
(0.2)
table continued..

-------
TABLE C.16. continued
Number
Condition of Deter- 	 Unit 1	 	Unit 2	 Unit 3
Number mlnatlons Influent Point A Point B Effluent Point A Point B Effluent Effluent
9
4
6.8
(0.2)
7.0
(0.2)
7.2
(0.2)
7.3
(0.2)
7.3
(0.1)
7.3
(0.1)
7.3
(0.1)

10
3
6.5
(0.2)
6.5
(0.1)
6.8
(0.1)
6.9
(0.1)
7.0
(0.1)
7.1
(0.1)
7.2
(0.2)

11
5
6.6
(0.2)
—
—
6.8
(0.1)
—
—
7.0
(0.2)
7.1
(0.2)
12
4
7.0
(0.2)
—
—
7.4
(0.3)
—
—
7.8
(0.2)
7.8
(0.2)
13
6
7.1
(0.1)
—
—
6.9
(0.2)
—
—
7.5
(0.1)
7.7
(0.1)
14
4
7.0
(0.1)
—
—
6.9
(0.2)
—
—
7.4
(0.1)
7.6
(0.1)
15
4
6.9
(0.2)
—
—
7.6
(0.1)
—
—
7.8
(0.1)
7.9
(0.1)
16
3
6.8
(0.2)
—
—
7.7
(0.2)
—
—
7.9
(0.1)
8.0
(0.1)

-------
TABLE C.17. FILTERED pH AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT SYSTEMS USING SYNTHETIC
WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.

Condition
Number
of Deter-

Unit 1

Unit
2
Unit 3
Number
minations
Influent
Point A Point B
Effluent
Point A Point
B Effluent
Effluent
1
2
3





4
—




5
6
6.5
6.7
7.1
7.2


(0.3)
(0.1)
(0.2)
(0.1)
6
5
7.0
7.1
7.5
7.4


(0.1)
(0.0)
(0.1)
(0.1)
7
3
7.2
7.2
7.4
7.3


(0.1)
(0.2)
	
(0.1)
8
5
6.8
7.3
7.2
7.3


(0.3)
(0.2)
(0.2)
(0.1)
table continued.

-------
TABLE C.17. continued
Numbe r
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
9
4
6.8
(0.1)
7.1
(0.2)
7.3
(0.1)
7.3
(0.1)
7.3
(0.1)
7.3
(0.1)
7.2
(0.1)

10
3
6.6
(0.2)
7.0
(0.3)
7.0
(0.1)
7.1
(0.2)
7.2
(0.3)
7.2
(0.2)
7.2
(0.2)

11
—
6.8
(0.2)
—
—
7.0
(0.2)
—
—
7.2
(0.2)
7.2
(0.1)
12
—
7.2
(0.2)
—
—
7.9
(0.5)
—
—
8.3
(0.3)
8.3
(0.3)
13
—
7.2
(0.2)
—
—
7.5
(0.4)
—
—
8.0
(0.2)
8.2
(0.2)
14
—
7.1
(0.1)
—
—
7.4
(0.5)
—
—
8.0
(0.3)
8.0
(0.1)
15
3
7.4
(0.2)
—
—
8.0
(0.4)
—
—
8.4
(0.4)
8.1
(0.2)
16
3
7.1
(0.1)
—
—
8.1
(0.1)
—
—
8.3
(0.1)
8.3
(0.1)

-------
TABLE C.18. DISSOLVED OXYGEN CONCENTRATIONS (mg/fc) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT
SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
¦p-
ro
¦p-
1
4
8.47
0.8
0.03
2.35


(1.0)
(0.4)
(0.06)
(1.18)
2
3
10.2
3.9
1.22
2.08


(0.5)
(0.7)
(0.79)
(1.05)
3
2
10.5
3.7
3.10
3.20


(1.3)
(1.0)
(2.68)
(2.54)
4
1
7.5
4.0
1.2
2.60
5
9
8.0
0.2
0.2
4.7


(1.2)
(0.3)
(0.4)
(2.1)
6
—




7
4
7.2
1.8
3.5
7.0


(1.3)
(1.3)
(1.8)
(0.9)
8
—




9
—




10
—




11
4
7.6
1.0
0.4
1.0


(0.6)
(0.6)
(0.1)
(1.2)
12
13
14
15
16
1.2
(1.5)
0.4
(0.2)
2.8
(1.7)
5.6
(0.6)

-------
TABLE C.19. TOTAL SOLIDS CONCENTRATIONS (mg/fc) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT
SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
1
3
660.0
(45.8)
450.0
(130.0)
786.7
(706.1)
393.3
(51.3)


2
4
652.5
(60.8)
497.5
(22.2)
425.0
(31.1)
380.0
(38.3)


3
4
642.5
(86.6)
630.0
(73.5)
572.5
(64.0)
570.0
(60.6)


4
3
723.3
(49.3)
703.3
(317.8)
560.0
(98.5)
443.3
(25.2)


5
6
7
8
616.2
(160.0)
530.0
(169.4)
311.2
(107.0)
358.8
(79.7)


8
9
10
—






11
6
428.3
(40.7)
—
—
440.0
(427.0)
258.3
(66.2)
253.3
(30.1)
12
5
420.0
(40.6)
—
—
338.0
(44.9)
388.0
(179.6)
278.0
(99.6)
13
3
383.3
(141.5)


336.7
(176.0)
240.0
(88.9)
233.3
(76.4)
14
15
16

-------
TABLE C.20. TOTAL VOLATILE SOLIDS CONCENTRATIONS (mg/A) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE
PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
1
3
433.3
(41.6)
223.3
(124.2)
580.0
(711.9)
176.7
(25.2)




2
4
450.0
(62.7)
290.0
(21.6)
202.5
(9.6)
160.0
(31.6)




3
4
427.5
(58.5)
410.0
(59.4)
360.0
(61.6)
350.0
(58.9)




4
3
476.7
(35.1)
450.0
(315.7)
310.0
(69.3)
193.3
(37.9)




5
6
7
8
452.5
(90.4)
390.0
(182.0)
173.8
(82.3)
215.7
(60.0)




8
9
10
—








11
6
238.3
(45.4)
	
—
235.0
(435.0)
	

90.0
(34.6)
81.7
(36.0)
12
3
213.3
(11.5)
	
—
133.3
(11.5)
	
	
150.0
(46.9)
110.0
(17.3)
13
4
207.5
(66.5)
	
—
160.0
(42.4)
	
	
120.5
(5.0)
87.5
(23.6)
14
4
120.0
(51.6)
	
—
127.5
(60.2)
	
——
105.0
(61.4)
67.5
(38.6)
15
4
110.0
(21.6)
	
—
127.5
(79.3)
	
——
182.5
(29.8)
220.0
(89.1)
16
4
445.0
(242.8)

__
176.7
(110.2)


187.5
(164.4)
110.0
(106.8)


-------
TABLE C.21. SUSPENDED SOLIDS CONCENTRATIONS (mg/£) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT
SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Condition
Number
of Deter-

Unit
1

Unit 2

Unit 3
Number
minations
Influent
Point A
Point B
Kf fluent
Point A Point B
Effluent
Effluent
1
3
2.7
(2.3)
21.3
(7.6)
20.7
(11.4)
5.3
(4.2)



2
4
4.0
(2.8)
27.8
(7.5)
11.0
(6.8)
9.5
(4.1)



3
4
2.0
(2.8)
10.5
(4.1)
11.0
(10.1)
12.0
(7.8)



4
4
1.0
(1.1)
11.5
(3.4)
22.0
(10.6)
11.5
(5.5)



5
8
4.5
(5.2)
14.2
(4.6)
14.0
(5.3)
7.8
(4.8)



6
7
8
9
10
—







11
6
4.3
(2.0)
—
—
7.7
(3.0)
— —
6.8
(1.8)
8.0
(10.1)
12
4
7.0
(4.8)
—
—
21.0
(14.1)
— —
14.0
(6.9)
9.5
(6.4)
13
3
8.0
(3.5)
—
—
17.7
(7.6)
— —
10.0
(6.0)
8.0
(5.3)
14
3
5.3
(2.3)
—
—
17.7
(15.0)
— —
13.3
(8.6)
11.7
(5.5)
15
—







16
5
11.2
(6.8)
—
—
33.8
(25.8)
	 	
53.3
(14.0)
40.4
(15.7)

-------
TABLE C.22. VOLATILE SUSPENDED SOLIDS CONCENTRATIONS (mg/£) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE
PILOT SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
1
3
2.7
(2.3)
21.3
(7.6)
18.0
(8.7)
5.3
(4.2)




2
4
2.0
21.7
(8.5)
7.2
(5.7)
8.0
(2.8)




3
4
1.5
(1.9)
9.5
(3.4)
10.0
(8.2)
11.0
(6.8)




4
4
0.5
(1.0)
7.0
(6.2)
14.5
(5.0)
8.5
(4.1)




5
6
8
4.0
(4.7)
13.2
(4.3)
12.8
(5.4)
6.5
(4.2)




7
8
9
10
—








11
6
3.7
(0.8)
	
—
6.3
(3.0)
—
—
6.0
(2.0)
2.0
(2.0)
12
4
5.5
(3.0)
	
—
17.5
(15.3)
—
—
9.5
(5.0)
8.5
(5.3)
13
4
4.5
(2.5)
	
—
17.8
(10.4)
—
—
14.2
(7.0)
9.8
(6.6)
14
3
4.7
(3.0)
	
—
13.0
(10.4)
—
—
12.3
(8.5)
9.3
(4.2)
15
3
44.7
(7.7)
	
—
9.3
(13.6)
—
—
14.5
(20.5)
5.3
(9.2)
16
3
6.3
(3.5)
::
—
20.2
(15.6)
—
—
37.3
(16.8)
24.5
(13.2)

-------
TABLE C.23. ZINC CONCENTRATIONS (mg/A) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT
SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
-p-
ro
vo
Number
Condition of Deter- 	Unit 1	 	Unit 2	 Unit 3
Number minations Influent Point A Point B Effluent Point A Point B Effluent Effluent
1
2
3
4
5	8	0.76	0.21	0.14	0.09
(0.25) (0.05) (0.11) (0.01)
6	4	0.35	0.17	0.11	0.10
7
8
9
10
11
12
13
14
15
16

-------
TABLE C.24. CADMIUM CONCENTRATIONS (mg/£) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT
SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Condi-
tion
Actual
Mass
Loading
Rate
No. of
Deter-
mina-

Unit
1

Unit 2
Unit 3
Number
kg/ha-d
tions
Influent
Point A
Point B
Effluent
Point A Point B Effluent
Effluent
1
.112
3
0.11
(0.03)
0.04
(0.02)
0.02
(0.01)
0.02
(0.02)


2
.112
3
0.11
(0.03)
0.07
(0.02)
0.02
(0.02)
0.01
(0.01)


3
.061
1
0.06
0.05
0.06
0.02


4
5
.168
6
0.33
0.10
0.06
0.05



(0.24)
(0.02)
(0.01)
(0.02)
6
.214
6
0.21
0.10
0.06
0.05



(0.06)
(0.04)
(0.01)
(0.01)
7
.076
4
0.15
0.08
0.06
0.06



(0.04)
(0.02)
(0.02)
(0.02)
8
.305
8
0.15
0.12
0.10
0.09



(0.03)
(0.03)
(0.03)
(0.03)
table continued...

-------
TABLE C.24. continued
Condi-
tion
Number
Actual
Mass
Loading
Rate
kg/ha-d
No. of
Deter-
mina-
tions
Unit 1
Unit 2
Influent Point A Point B Effluent Point A Point B Effluent
Unit 3
Effluent
.193
-p-
0.19
(0.15)
0.13
(0.05)
0.10
(0.04)
0.09
(0.04)


4




0.08
(0.04)
0.07
(0.03)
0.06
(0.04)

10
.509
3
0.25
(0.11)
0.15
(0.08)
0.12
(0.05)
0.11
(0.06)
0.10
(0.06)
0.10
(0.05)
0.11
(0.06)

11
.020
6
0.03
(0.02)
—
—
0.01
(0.02)
—
—
0.01
(0.02)
0.003
(0.01)
12
.183
5
0.18
(0.04)
—
—
0.14
(0.04)
—
—
0.10
(0.03)
0.05
(0.01)
13
.122
5
0.06
(0.03)
—
—
0.04
(0.01)
—
—
0.02
(0.01)
0.02
(0.02)
14
15
16

-------
TABLE C. 24. SOLUBLE CADMIUM CONCENTRATIONS (mg/£) AT VARIOUS POINTS IN THE CORNELL BRACEHOUSE PILOT
SYSTEMS USING SYNTHETIC WASTEWATER. STANDARD DEVIATIONS ARE SHOWN IN PARENTHESES.
Condi-
tion
Actual
Mass
Loading
Rate
No. of
Deter-
mina-

Unit 1


Unit 2

Unit 3
Number
kg/ha-d
tions
Influent
Point A Point B
Effluent
Point A
Point B
Effluent
Effluent
1
2
3
4
5
6
7
8
9
10

__







11
.014
6
0.02
(0.02)
—
0.01
(0.01)
—
—
0.01
(0.01)
0.01
(0.01)
12
.173
5
0.17
(0.05)
—
0.09
(0.05)
—
—
0.04
(0.01)
0.03
(0.02)
13
.122
5
0.06
(0.01)
—
0.03
(0.01)
—
—
0.02
(0.02)
0.01
(0.01)
14
16

-------
50 0
5"-i0
>-iB 0
1-.5 0
BB8
30 0
SU0
•1 BQ
•1S0
E 0
0
0 10 E0 30 L-i 0 5 0 B0 70 B 0 3 0-100
IK
.. V

\ .<
a
A H
' ^ 1
l	
-------
TIME, days
Figure C.3 Chemical oxygen demand concentration
of synthetic sewage at Point B
in the Cornell NFT in PEase 0. First
day of analysis was December 5, 1979.
i
in'-i i-'j
- £ -£i j-
0 '
.50 0 4-
I
EL- 8 4-„
l E 0 ^
i
•1 cz; 0 4-
E0 --
.--3
i3-a—-3_

l~'i -i-
-I
0 "1 0 20 13 0
0
50
T-V'
3 0 3u -'1 00
TIME, days
Figure C.4 Chemical oxygen demand concentration of
effluent synthetic sewage from the
Cornell NFT in Phase 0. First day of
analysis was December 5, 1979.
U34

-------
- ?¦
T/\
50 0
B1-! 0
LiB0
350 --
30 0 --
E^0..
••130-
1 50 --
E0-

a s
_-a. / \
\/
13"	s
•o"
0
0 -10 50 30 Li 0 5 0 B0 70 30 30 -100
TIME, days
Figure C.5 Soluble oxygen demand concentration of
influent synthetic sewage to the Cornell
NFT in Phase 0. First day of analysis
was December 5, 1979.
By 0
5i-i 0
L-.B 0
L-i5 Q --
riS 0 --
30'0 -tf],
iti
E1-. t:i -L
¦1-3 0}
1 e 0 •!•

~
2

• 1 I
~-j' >
a	l
i '

•-! "I

1- tl
Sj'tJ	r-hj
S I 00
TIME, days
Figure C.6 Soluble oxygen demand concentration
of synthetic sewage at Point A in the
Cornell NFT in Phase 0. First day of analysis
was December 5, 1979.
U35

-------
TIME, days
Figure C.7 Soluble oxygen demand concentration
of synthetic sewage at Point B
in the Cornell NFT in Phase 0. First day
of analysis was December 5, 1979.
5f 0
5i-iQ
1-13 0 --
>-12 0
35 0 -¦
3O0
SL-10 -¦
-1 S3 u1
•13 0 --
50 --
0
3—^.
f
*3-
R""*

•y
Pk /
.-d ^
"'as
"s-er"
0 -10 EU 30 "-i0 5Gi E0 70 B0 3 0 -100
TIME, days
Figure C.8 Soluble oxygen demand concentration of
effluent synthetic sewage from the
Cornell NFT in Phase 0. First day of
analysis was December 5, 1979.
U36

-------
5 0
L-lS
*—I 0
35
30
E5
E 0
1 5
'I 0
5
0
0 -10 E0 30 L-i0 5 0 E0 ?0 50 50 '100
A
-• X	--h' \	Pv
' X	rJ3'	\	>'	ja
l " ~ —Q- ''
H	(1111(1	H
TIME, days
Figure C.9 Total nitrogen concentration of
synthetic sewage to the Cornell NFT
in Phase 0. First day of analysis was
December 7, 1979.
E 0
L-,0
35
30
E5
E0
•1 5
1 0
5
Q
0 'I© E0 30 L-i 0 5 0 50 70 5 0 50 100
TIME, days
Figure C.10 Total nitrogen concentration of synthetic
sewage at Point A in the Cornell NFT in
Phase 0. First day of analysis was
December 7, 1979.
U3T

-------
5 0
^5
L-i 0
35
3 0
as
a a
-1 5
•1 0
5
0
0 "10 20 30 1-10 50 E0 70 30 30 100
TIME, days
Figure C.ll Total nitrogen concentration of synthetic
sewage at Point B in the Cornell NFT
in Phase 0. First day of analysis was
December 7, 1979.
-1 0
J

r : -J
TIME, days
E'3 -1 ui-1
	
Reproduced from
best available copy,
Figure C.12 Total nitrogen concentration of effluent
synthetic sewage from Cornell NFT in
Phase 0. First day of analysis was
December 7, 1979.
i+38

-------
50
h5
l-i 0
35
30
55
20
•1 5
1	0
5
0
0 'I© 20 30 l-i0 50 G0 70 50 50 '100
TIME, days
Figure C.13 Soluble total nitrogen concentration of
influent synthetic sewage to the Cornell
NFT in Phase 0. First day of analysis
was December 7, 1979.
50
L-i 5
1-10
35
30
25
2	0
•1 5
1 0
5
0
0 "10 20 30 !-l0 5 0 50 70 B0 5 0 -100
TIME, days
Figure C.14 Soluble total nitrogen concentration of
synthetic sewage at Point A in the Cornell
NFT in Phase 0. First day of analysis
was December 7, 1979.
R
U
- ¦ V
Xi V
VC3''
JBf
/


-I	1-
t	I
U39

-------
50 T
'-i 5 1
I
L-i 3
35
3 3
T
a—
--a"' \
i
ef" L-:*——"9.
^		a
UJ	i
2 0 -I
1 5
•1 Q +
3 -j	1	1	i	f	1 t	H
I
I
I
-t-
33 i-!*-: !aW ta3 'r -J 3<-i 3 0 ¦] fc!j
TIME, days
Figure C.15 Soluble total nitrogen concentration of
synthetic sewage at Point B in the
Cornell NFT in Phase 0. First day of
analysis was December 7, 1979.
b 0 -i-
-I .'j I
L-.0
35
- - a.
3h3--.
3
•23''"
—3 ,ET"
£T
: 3 -L
1 5 ¦
1 0
5
0 -I	1	t	\	I	1	!	1	1	1-
Hl! • | '^i £53 3 0 *—1 kii ^ i J ^3 y "? w 3 3 3 U i 'ct 0
TIME, days
Figure C.16 Soluble total nitrogen concentration of
effluent synthetic sewage from Cornell NFT
in Phase 0. First day of analysis was
December 7, 1979.


-------
l-i -J 'tL .

25;
3 0 i
1
"I
•1 0 -j-
s +
1
0 4-
0
—3'
rr~
3
bS
^'3-„
0
50 3y
it)
!a o
a iTi .-1 utui
TIME, days
Figure C.17 Total Kjeldahl Nitrogen concentration of
influent synthetic sewage to the Cornell
NFT in Phase 0. First day of analysis
was December 7, 1979.
E 0
L-S '
r
a	ft	b
30 -¦
E 0 4-
•15-
-1 9 !
E i
.-a \
a.
t
I /	'
: t	1
a
k
!
3
•jJ -	—'-J *—' K^l 'zz •-1 C3 rlj V V J 3 III 5^ '"i
TIME, days
Figure C.18 Total Kjeldahl nitrogen concentration
of synthetic sewage at Point A in the
Cornell NFT in Phase 0. First day of
analysis was December 7, 1979.
UUl

-------
50
•-i 5
L-,0
35
30
35
30
-1 5
1 0
5
0
0 'I© 30 30 *-10 50 G0 70 30 30 '100
TIME, days
Figure C.19 Total Kjeldahl Nitrogen concentration
of synthetic sewage at Point B in the
Cornell NFT in Phase 0. First day of
analysis was December 7, 1979.
5 0
L-i 5
L-i 0
35
30
35
3 0
•1 5
•1 0
5
0
0 10 30 30 L-10 5 0 E0 70 B0 3 0 -100
TIME, days
Figure C.20 Total Kjeldahl Nitrogen concentration of
effluent synthetic sewage from Cornell
NFT in Phase 0. First day of analysis
was December 7, 1979.


m.

Cr"
"GJ .53-—-Q
\ x-"
ta
1+42

-------
30 1 00
TIME, days
Figure C.21 Soluble total Kjeldahl nitrogen concentration
of influent synthetic sewage to the Cornell
NFT in Phase 0. First day of analysis was
December 7, 1979.
5 0 		
•	i

i— i-H
i s 4
r
i
TIME, days
Figure C.22 Soluble total Kjeldahl nitogen concentration
of synthetic sewage at Point A in the
Cornell NFT in Phase 0. First day of analysis
was December 7, 1979.
U43

-------
50 -j-
«-iS --
L-10 --
35
30 --
55--
30-.
15-
-1 0--
5 ¦¦
0
0
w
A

*10 30 30 ^0 50 50 70 B0 50 A 0fcJ
TIME, days
Figure C.23 Soluble total Kjeldahl nitrogen concentration
of synthetic sewage at Point A in the Cornell
NFT in Phase 0. First day of analysis was
December 7, 1979.
50
*i5
>-10
35
30
35
30
-1 5
•1 0
5
0
f "1 fcJ 30 3bJ '-10 5 0 E0 70 3 0 50 100
TIME, days
Figure C.24 Soluble total Kjeldahl nitrogen concentration
of effluent synthetic sewage from Cornell NFT
in Phase 0. First day of analysis was Dec-
ember 7, 1979.


-------
50
00
6
z
I
u
o
06
O
hd
•10 3td 30 *—i kJ 5tl Em 70 B0 3 0 -100
TIME, days
Figure C.25 Organic nitrogen concentration of influent
synthetic sewage to the Cornell NFT in
Phase 0. First day of analysis was
December 7, 1979.
5 0
60
a
z
I
o
OS
O
L-J
EEIy 231 '—1 0

r 'cj
3 0 3 0 -1
TIME, days
Figure C.26 Organic nitrogen concentration of synthetic
sewage at Point A in the Cornell NFT in
Phase 0. First day of analysis was
December 7, 1979.
UU5

-------
si
01-
!
—-a
'.-J
—'a--
i,
¦.¦J
y
•1 !:-j 20 By L-i9 5u by r"y By 3 0 -1 yy
TIME, days
Figure C.27 Organic nitrogen concentration of synthetic
sewage at Point B in the Cornell NFT in
Phase 0. First day of analysis was
December 7, 1979.
5 0
y By ?y BG By -i mm
TIME, days
Figure C.28 Organic nitrogen concentration of effluent
synthetic sewage from Cornell NFT in Phase 0.
First day of analysis was December 7, 1979.
kkS

-------
0 -10 20 30 L-i 0 50 SfcJ t7© ~ 0 9 0 -1 U0
TIME, days
Figure C.29 Soluble organic nitrogen concentration of
influent synthetic sewage to the Cornell
NFT in Phase 0. First day of analysis was
December 7, 19 79.
5 0
hS-
!—10 --
3 5--
30-
/ I
3 5--	/ l|
!	
-------
00
a
z
i
u
o
OS
o
hJ
o
to
S8i
3 E
2 -i-
i
25-
SQ --
1 5 --
•1 0 --
^ it

-a	bh
i£
/I
i
13-—
'1
I
-H

W '113 2^J 30 *-i fcJ 5Eti 7ti BQ 3u 1 tiid
TIME, days

CO
6
z
I
u
o
oS
o
o
to
Figure C.31 Soluble organic nitrogen concentration of
synthetic sewage at Point B in the Cornell
NFT in Phase 0. First day of analysis was
December 7, 1979.
5 0
1 td 20 « 30 L-i 0 S tl E0 70 0 0 30 '1 £
TIME, days
Figure C.32 Soluble organic nitrogen concentration of
effluent synthetic sewage from Cornell NFT
in Phase 0. First day of analysis was
December 7, 1979.
UU8

-------
I—! 0
3E
3S
EB
ah
EG
'1 S
-1 E
B
0
0 10 E0 30	50 E0 70 B0 E0 '100
TIME, days
Figure C.33 Ammonia nitrogen concentration of influent
synthetic sewage to the Cornell NFT in
Phase 0. First day of analysis was
December 7, 1979.
L-.0
3E
EE
EB
Eh
50
•1 S
•1 E
B
i-.
0
0 10 50 30 Li0 50 E0 70 B0 90 '100
TIME, days
Figure C.34 Ammonia nitrogen concentration of synthetic
sewage at Point A in the Cornell NFT in
Phase 0. First day of analysis was
December 7, 1979.




A

XT3''
a?

i 	
\
1
\ / \
\ /
*a
i i i i i
P
""X
1 1 1
kb9

-------
1-10

a i-i -¦

00

a
a 0 - -
z
1
-1 s..
CO

§
1 a --

B -¦

--
G -1t) E0 3G i—10 5 0 E0 70 B0 E30 -10y
TIME, days
00
0
Figure C.35
i—10
Jb-
35 -
EB -k
ai-i --
E 0 +
•1 B
•1 a +
z
i
aT a +
z
4"
Ammonia nitrogen concentration of synthetic
sewage at Point B in the Cornell NFT in
Phase 0. First day of analysis was December
7, 1979.
' .3
P
<^S3r"
3-~
•1 0	3'"I 1-1 V-J !:J (3 0
TIME, days
¦'-j a u
! 0 1
Figure C.36 Ammonia nitrogen concentration of effluent
synthetic sewage from Cornell NFT in Phase 0.
First day of analysis was December 7, 1979.
U50

-------
fcj
•1 0
20 3tJ '—iU St-J ES 7Q B0 3 0 '100
TIME, days
Figure C.37
Soluble ammonia nitrogen concentration of
influent synthetic sewage to the Cornell
NFT in Phase 0. First day of analysis was
December 7, 1979.
oo
E
Z
I
o
L,0
3E --
32 --
SB --
E >-ic —_
50 -¦
•1 5 --
•1 S --
a -•
i-i
0	
0


p
.a
\
ta
"10 5 Id 30 L-ttJ
i id SfcJ 70 B 0 9u 100
TIME, days
Figure C.38
Soluble ammonia nitrogen concentration of
synthetic sewage at Point A in the Cornell
NFT in Phase 0. First day of analysis was
December 7, 1979.
^51

-------
0 E?Q 3ti L-iO btt E0	Bti 3tJ -100
TIME, days
Figure C.39
Soluble ammonia nitrogen concentration of
synthetic sewage at Point B in the Cornell
NFT in Phase 0. First day of analysis was
December 7, 1979.
TIME, days
—			aaitfy
Figure C.40 Soluble ammonia nitrogen concentration of
effluent synthetic sewage from Cornell NFT
in Phase 0. First day of analysis was
December 7, 1979.
U52

-------
1 0 T-
1
I
3 f
5 1
3 -
'h.
J. "
i


!3
a
. I
1 0

:Q ay 3 0 1 yi
TIME, days
Figure C.41 Nitrate nitrogen concentration of influent
synthetic sewage to the Cornell NFT in
Phase 0. First day of analysis was
December 7, 1979.
3 f
S3
5
^3
I
i
4.
I—-EL.
a
—i-

i3-..
/ a—Q
-T-l£	h-
I	I
W 10 SO 30 Md :3ti b0
TIME, days
r0 Bu 9 0 1 y8
Figure C.42 Nitrate nitrogen concentration of synthetic
sewage at Point A in the Cornell NFT in
Phase 0. First day of analysis was December
7, 1979.
453

-------
1 --
0 »
,J3..
i a
o"S7"~~R-a o
or
-t-d~—y-
13 I
0 -10 E0 30 1-10 5 0 E0 70 B0 3 0 -11)0
TIME, days
Figure C.43 Nitrate nitrogen concentration of synthetic
sewage at Point B in the Cornell NFT in
Phase 0. First day of analysis was
December 7, 1979.
1 0
J"
B-a
TIME, days
3 0 -1 00
Figure C.44 Nitrate nitrogen concentration of effluent
synthetic sewage from the Cornell NFT
in Phase 0. First day of analysis was
December 7, 1979.


-------
TIME, days
Figure C.45 Soluble nitrate nitrogen concentration of
influent synthetic sewage to the Cornell
NFT in Phase 0. First day of analysis was
December 7, 1979.
1	0
~
B
"?
S
5
L-t
3
2
•1
0
0 1 G 20 30 i-i0 5 0 50 "7Q 3 0 3 0 -1 08
TIME, days
Figure C.46 Soluble nitrate nitrogen concentration of
synthetic sewage at Point A in the Cornell
NFT in Phase 0. First day of analysis
was December 7, 1979.
^55

-------

¦•1 0

~

B

7
~oo
E
5
Z
5
1
|
L-.
I

o
3
z

~J
a
o

to


1
W-
~ 0 'I 00
Figure C.47
TIME, days
Soluble nitrate nitrogen concentration of
synthetic sewage at Point B in the Cornell
NFT in Phase 0. First day of analysis was
December 7, 1979.

1 0

3


co
B
E

*
7
z

I
E
I
fO
o
5
z

P-J
i-i
o

w
3

a

-1
1 0
30 i-. 0 5 0
TIME, days
3fcJ 1 00
Figure C.48 Soluble nitrate nitrogen concentration of
effluent synthetic sewage from the Cornell
NFT in Phase 0. First day of analysis was
December 7, 1979.


-------
20
1 a
1 s
•1 L-,
1	a
•1 0
B
E
i-i
2
0
0 '10 20 30 L-i0 50 E0 70 50 90 '1 fclfcl
TIME, days
Figure C.49 Orthophosphate concentration of influent
synthetic sewage to the Cornell NFT in
Phase 0. First day of analysis was
December 5, 1979.
20
t B
•1 E
1
-1 2
1	Q
B
E
L-.
2
0
0 10 20 30 l-i0 5 0 E0 r"0 3 0 9 0 -1 W0
TIME, days
Figure C.50 Orthophosphate concentration of synthetic
sewage at Point A in the Cornell NFT in
Phase 0. First day of analysis was
December 5, 1979.

-------
E 0
•1 a
1 B
1 l-i
•1 a
-1 0
B
B
"-I
E
0
0 '10 E0 30 Li 0 5 0 E0 70 B 0 3 0 '100
TIME, days
Figure C.51 Orthophosphate concentration of synthetic
sewage at Point B in the Cornell NFT in
Phase 0. First day of analysis was
December 5, 1979.
E 0 ,	
a
i
i
t
I
i	1			1			1	1
TIME, days
Figure C.52 Orthophosphate concentration of effluent
synthetic sewage from the Cornell NFT in
Phase 0. First day of analysis was
December 5, 1979.
U58

-------
3 0
•1 B
1 E
•1 L-,
•1 a
'1 0
B
E
i-.
a
0
0 '10 E0 30 i-i0 50 E0 70 B0 30 100

A
A /
I	I
V
V"
V
i	t	1	1	1	1	i	1	»-
TIME, days
Figure C.53 Soluble orthophosphate concentration of
influent synthetic sewage to the Cornell
NFT in Phase 0. First day of analysis was
December 5, 1979.
i^r	V r
L
K, j
B ..	\
M
1-1 --
a --
0 -I	1 I	1	1	1 I	1	1 I	
0 -10 30 30 1-10 5 0 EQ 70 B0 3 0 -100
TIME, days
Figure C.54 Soluble orthphosphate concentration of
synthetic sewage at Point A in the Cornell
NFT in Phase 0. First day of analysis was
December 5, 1979.
NFT m Phase 0. First day of analysis
December 5, 1979.
r'
J3-
(a.
¦+! _-EJ
! \



\ ^
\





1 »
»
i i » i i i
U59

-------

•1 l-. or
00
a
i E --
cu
••1 0 --
i

-------
B5 0
7B5
-SB0
53 5
5-1 0
L-i2 5
3h0
555
1 7 0
as
0
0 '10 20 30 ^-10 50 50 70 B0 50 100
TIME, days
Figure C.57 Total solids concentration of influent
synthetic sewage to the Cornell NFT in
Phase 0. First day of analysis was
December 5, 1979.
•1 1 Q 0
~30
BB0
770
BE 0
55 0
•—i1—10
330
550
•1 -1 0
0
0 10 30 30 L-i0 5 0 50 70 BO 3 0 -100
TIME, days
Figure C.58 Total solids concentration of synthetic
sewage at Point A in the Cornell NFT in
Phase 0. First day of analysis was December
5, 1979.
U6i

-------
•1 70 M
1 530
' -E0 -r I	V
i	y
1 13Q4- /	\
1 0C0 ¦¦ ,1
BE Q -¦ .1
/ \
^ SB 0 --
oo
6
5 1 0 - - i1
n I
£ 3^0
1 7 0-
is-
-q q—a"
.13-"
	-Q
J3—EU
n

0 '10 20 30 >-10 5.0 50 70 E 0 3 0 100
TIME, days
Figure C.59 Total solids concentration of synthetic
sewage at Point B in the Cornell NFT
in Phase 0. First day of analysis was
December 5, 1979.
60
6
C/3
H
E00 --
EEI5 ¦¦
S-1 0 --
UES*
BM-J --
	
:di
0

=:ta :=a
•17 0-
as -
-a-a—cT
0 4-
H	1	1	I
-)	1	1	I-
i--1 • ¦] r_l 2 ¦_! 31J ^—1 0 ^3 I-1.1 ^3 7 3 !'-J ri ' '1 Ll^
TIME, days
Figure C.60 Total solids concentration of effluent
synthetic sewage from the Cornell NFT in
Phase 0. First day of analysis was
December 5, 1979.
4d2

-------
05 0
7ES-.
BS0 ¦¦
sas..
5-1 0
M25|
3Li0 !i
255 -¦
¦170-
as-
ja.
'Q"
Pv
'""d

a
0 10 20 30 "-.0 50 E0 70 30 30-100
TIME, days
Figure C.61 Total volatile solids concentration of
influent synthetic sewage to the Cornell
NFT in Phase 0, First day of analysis was
December 5, 1979.
050
755 --
EB0 --
535--
51	0 -¦
Mas --
31-10
S55
¦'1 7 0
0 5
4A
t-i
\ /
a
M

,aEr"
T3-"
I I,
I I
23
0 -1 9 50
—I	1	1	1	1	1	1	
3 0 HO 5 0 E0 70 B0 3 0 -1 00
TIME, days
Figure C.62 Total volatile solids concentration of
synthetic sewage at Point A in the Cornell
NFT in Phase 0. First day of analysis was
December 5, 1979.
U63

-------
-1 70 0
•1 53 0
•1 35 0
1 '13 0
•1 Q30
B50
EB0
5-1 0
3Li0
-17 0
0
0 -10 30 30 L-i 0 50 50 70 3 0 30 -100
TIME, days
Figure C.63 Total volatile solids concentration of
synthetic sewage at Point B in the Cornell
NFT in Phase 0. First day of analysis was
December 5, 1979.
B5 0
7B5
G3 0
53 5
5-1 0
L-i35
3>-i0
35 5
17 0
B 5
0
fcj 1 y Et.1 3k) '-i 0 5t) 50 70 B 0 3 0 -1 00
TIME, days
Figure C.64 Total volatile solids concentration of effluent
synthetic sewage from the Cornell NFT in
Phase 0. First day of analysis was
December 5, 1979.
U6U

-------
E50
755
EB0
535
5-1 8
>-tS5
3«i 0
355
•1 7 0
B 5
0
0 10 30 30 l-i0 50 50 70 50 30 100
TIME, days
Figure C.65 Total fixed solids concentration of
influent synthetic sewage to the Cornell NFT
in Phase 0. First day of analysis was
December 5, 1979.
55 0
755
SB 0
535
5-1 0
1-.E5
31-10
E55
•1 7 0
35
0
0 -10 E0 30 >-10 5 0 50 70 B 0 3 0 -100
TIME, days
Figure C.66 Total fixed solids concentration of
synthetic sewage at Point A in the Cornell
NFT in Phase 0. First day of analysis was
December 5, 1979.
P ~ —.

--S-—_r3__a-ae'
_-a
-i	1	i	t	t	i	1	1	1-
465

-------
1 70 0
1 530
1 3E0
1-190
1 03 0
550
55 0
5-1 0
170
0 'I© 20 30 *-i0 50 StJ 70 50 50 10bJ
TIME, days
Figure C.67
Total fixed solids concentration of
synthetic sewage at Point B in the Cornell
NFT in Phase 0. First day of analysis was
December 5, 1979.
B5S
EB >d --
535 ..
70-
t.i
-•a-. .
-e-er"
[a...
_cr

"-12T

.-•¦a
1 U 30
3a >-10 5 0
TIME, days
Gy 7t3 3 0 5 0 I QkJ
Figure C.68 Total fixed solids concentration of effluent
synthetic sewage from the Cornell NFT in
Phase 0. First day of analysis was
December 5, 1979.
U66

-------
jy

E7
--
5L-.
-•
a-1

1 B
-•
•1 3
--
•i a
--
3
-¦

ci
a
)

d
+
.3-
0 +&¦
0
-t-
-h
C3
I l,
I '
1
\ ,P-
£3—d
	1	i	
R.
13-3.
i '"ct—i tin—a-
-t-e-
10 S0 30 L-i 0 5 0 E0 ?tt By 3 tJ 1 yy
TIME, days
Figure C.69 Total suspended solids concentration of
influent synthetic sewage to the Cornell NFT
in Phase 0. First day of analysis was
December 5, 1979.
TIME, days
Figure C.70 Total suspended solids concentration of
synthetic sewage at Point A in the Cornell
NFT in Phase 0. First day of analysis was
December 5, 1979.
U6T

-------
1-,0-p
3B--
0 -I	1	t	1	i	i	1	i	1	1	
0 10 20 30 L-i0 50 E0	70 B0 3 0 A 00
TIME, days
Figure C.71 Total suspended solids	concentration of
synthetic sewage at Point B in the Cornell
NFT in Phase 0. First	day of analysis was
December 5, 1979.
30-r
57 --
Eh --
0 I	I	I	I	I	I	1	>	*	I
0 10 20 30 '-i© 5 0 E0 70 00 3 0 100
TIME, days
Figure C.72 Total suspended solids concentration of effluent
synthetic sewage from the Cornell NFT in
Phase 0. First day of analysis was December 5,
1979.
H68

-------
TIME, days
Figure C.73 Volatile suspended solids concentration of
influent synthetic sewage to the Cornell NFT
in Phase 0. First day of analysis was
December 5, 1979.
a a r
32 f
sal •
i
E'- i
EE 0 i
' 1 rz3 -4^
•I
•1 a 13
B
"-a
¦	i
Li
-4—	—4-

V
	iSS_i
0 10 EQ 30 >-10 5l.-1	70	"5'? ¦' H.:l
TIME, days
Figure C.74 Volatile suspended solids concentration of
snythetic sewage at Point A in the Cornell
NFT in Phase 0. First day of analysis
was December 5, 1979.
469

-------
00
e
Vi
w
>
L-,0
3E--
32 --
SB --
2<-.
E0
'1 5
1 E
5
0
.5?
r
\
/
\/ \
a
\
I

"A
\K
\ i ^
V
I	I	t	t	I	1	I	1	1-
0 'I© EG 30 1-i0 50 E0 70 0 0 3tl '100
TIME, days
Figure C.75 Volatile suspended solids concentration of
synthetic sewage at Point B in the Cornell
NFT in Phase 0. First day of analysis was
December 5, 1979.

30 -p

a?..

3L-. --

E-1 --

1 B --

•1 5 -¦
00
S
'1 E --
vss,
3 -¦
E
3 -
I
/ \

, - f\I
/ V / V /


\ / X I
"a
r
0
-I	1	1	t
¦H	1-
0 10 E0 30 1-10 5 0 E0 70 0 0 3 0 100
TIME, days
Figure C.76 Volatile suspended solids concentration of
effluent synthetic sewage from the Cornell
NFT in Pbase 0. First day of analysis was
December 5, 1979.
Uto

-------
'ri
— L.,
5 f
• i ^ -i-
-X.
• li' bn '-j :;i r '•_¦• ¦ i: 3 ••_' ! '¦-i * .'J
TIME, days
Figure C.77 Fixed suspended solids concentration of influent
synthetic sewage to the Cornell NFT in Phase
0. First day of analysis was December 5, 1979.

TIME, days
Figure C.78 Fixed suspended solids concentration of
synthetic sewage at Point A in the Cornell
NFT in Phase 0. First day of analysis was
December 1979.
U71

-------
1—( 0
35
35
SB
Ei-.
E0
•1 E
•1 E
B
Q
0 '10 20 30 '-i0 50 E0 70 B0 30 '100
TIME, days
Figure C.79 Fixed suspended solids concentration of
synthetic sewage at Point B in the Cornell
NFT in Phase 0. First day of analysis
was December 5, 1979.
30
E?
5i-i
E-1
-1 B
'1 5
1 E
3
E
3
0
0 -10 30 30 >-i0 50 E0 70 00 3 0 -100
TIME, days
Figure C.80 Fixed suspended solids concentration of
effluent synthetic sewage from the Cornell NFT
in Phase 0. First day of analysis was
December 5, 1979.
i

-------
E1T
i a i.
i s i
eh=j
-1 0 • -
0 B -
0E-
Qi-i ffl
B-
rr~
0 E 4ji
0 4—
0
&—si

Ei-. 3E l-i 0 »-.B EE E'-
7 E B0
TIME, days
Figure C.81 Cadmium concentration of influent synthetic
sewage to the Cornell NFT in Phase 0. First
day of analysis was December 7, 1979.
20
O a IE El-. 3E mil L-iB
TIME, days
E !~i
EG
Figure C.82 Cadmium concentration of synthetic sewage
at Point A in the Cornell NFT in Phase 0.
First day of analysis was December 7, 1979.

-------
0 = 20
0 a 1 B
0 a 1 E
0 a '1 l-l
0 a 1 2
0 „ "1 0
"m 0 n 0 B
e
" 0 a 0 E
Q
W ~tJU
0 »y3
0
0 B -IE 21-. 35 *-i 0 I-.B EE Eh 75 B0
TiME, days
Figure C.83 Cadmium concentration of synthetic sewage
at Point B in the Cornell NFT in Phase 0.
First day of analysis was December 7, 1979.
0.E0
0 „ "1 B
hi a 1 E
0 = m
0 a -1 a
si s o -1 0
00
E 0 ~ 0 B
8 t1 a 0 E
0 a 0 1-1
0»05
0
O B -IE E1-) 3 2 h0 hB EE Eh 7 2 BS
TIME, days
Figure C.84 Cadmium concentration of effluent synthetic
sewage from the Cornell NFT in Phase 0.
First day of analysis was December 7, 1979.

-------
TIME, days
Figure C.85 pH of influent synthetic sewaee to the
Cornell NFT in Phase 0. First day of
analysis was December 7, 1979.
TIME, days
Figure C.86 pH of snythetic sewage at Point A in the
Cornell NFT in Phase 0. First day of
analysis was December 7, 1979.
V75

-------
5 4
0
	1	I	I	1	1	1	I—
'11) 20 3tJ 1-i0 5 0 B0 70
t	i
B9 90 1 00
TIME, days
Figure C.87 pH of synthetic sewage at Point B in the
Cornell NFT in Phase 0. First day of
analysis was December 7, 1979.
•1 0 y
"1 0 -.
~ --
~ --
a -¦
B -¦
E -•
E --
E -I	1	1	1	1 i	1	1	1 i
0 -10 30 30 l-i 0 S0 E0	70 B0 3 0 100
TIME, days
Figure C.88 pH of effluent synthetic	sewage from the
Cornell NFT in Phase 0.	First day of
analysis was December 7,	1979.
kl6

-------
B
~r
7 i
			— ^	j'Srj	_ 		
I	^
t
1
H	1		1	I-
M ••']	31: J 1 1^.1 lz3 0 fcu f 0 Q 0 9 L_1 '1 t-1 M
TIME, days
Figure C.89 Soluble pH of influent synthetic sewage to
the Cornell NFT in Phase 0. First day of
analysis was December 7, 1979.
-1 0
•1 ©
3
~
a
B
7
7
B
E
3
0 10 EEQ 301 L-i 0 5 01 E0 70 BQ 30 100
TIME, days
Figure C.90 Soluble pH of influent synthetic sewage at
Point A in the Cornell NFT in Phase 0. First
day of analysis was December 7, 1979.

ET
UTT

-------
p..
' *			_s	/
7 ..	"--ei	^3"
E --
5
Q -10 E0 30 ^ 0 50 50 70 B0 30 100
TIME, days
Figure C.91 Soluble pH of synthetic sewage at Point B in
the Cornell NFT in Phase 0. First day of
analysis was December 7, 1979.
1 0 -p
'10-
3 --
3 --
B --
3 -¦
5 -¦
5 -¦
5 	1	1	h-	1	1	1	(	(	1	
0 -'10 30 30 1-10 5 0 50 70 30 3 0 100
TIME, days
Figure C.92 Soluble pH of effluent synthetic sewage from
the Cornell NFT in Phase 0. First day of
analysis was December 7, 1979.
1+78

-------
•1 0 0 - -
~ E --
?a --
E0 -¦
3E --
Sy 'i0 EG By I yy -1 2ti 1 '-iw 1 Ed '1 By 2ut
TIME, days
Figure C.93 Minimum daily air temperature in the Cornell
NFT greenhouse in Phase 0. First day of
measurement was November 12, 1979.
u
W
H
-1 E 0
1 0 B 4-

l
k,LMUll ^
zmw&mr #n.
;M '—iill EEif'-il 3 >-1.1 *1 LJ 1 ilE1"1 '—ikJ ¦] E kJ 1 E 'J
TIME, days
Figure C.94 Maximum daily air temperature in the Cornell
NFT greenhouse in Phase 0. First day of
measurement was November 12, 1979.
U79

-------
'1 5
1 (
1 a
•1 -i
3
B]
E
5
3
a
0
0 7 -13 50 SB 33 33 <-tE 53 53 E5
TIME, days
Figure C.95 Dissolved oxygen concentration of influent
synthetic sewage to the Cornell NFT in
Phase 0. First day of analysis was
December 18, 1979.
-1 5
1 L-.
•1 a
•1 -1
3
B
E
5
3
a
0
Q 7 "13 a0 as 33 33 UE 53 53 E5
TIME, days
Figure C.96 Dissolved oxygen concentration of synthetic
sewage at Point A in the Cornell NFT in Phase 0.
First day of analysis was December 18, 1979.
U80

-------
13 S0 EE 33 33 i-iE 53 53 E5
TIME, days
Figure C.97 Dissolved oxygen concentration of synthetic
sewage at Point B in the Cornell NFT in Phase 0.
First day of analysis was December 18, 1979.
V
TIME, days
53 E5
Figure C.98 Dissolved oxygen concentration of effluent
synthetic sewage from the Cornell NFT in
Phase 0. First day of analysis was
December 18, 1979.
USl

-------
50 0
SMS
>-iB kJ
Li50
350
no
a 30 0
*
o EI-.0
o
100
• 1 2 0
5 ^
0
0 S0 1-.0 50 B0 -100 '1S0 me '1E0 -100 S00
TIME, days
Figure C.99- Chemical oxygen demand concentration of
influent synthetic sewage delivered tc
the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980•
MA A
l \

_i	1	t	>	i	1	t	•-
E; I d >.1 ' I i V < 1 .i''j ' I '-f1J • 'I L= 'il' "IG'J
TIME, days
Figure C.100. Chemical oxygen demand concentration
of influent synthetic sewage at Point A
in the reed canary grass Cornell NFT
in Phase 1. First day of analysis was
March 19, 1980.
482

-------
50 0
5U0
H3 0
^lE 0
bD 2B 0
a
*	30 0
o
8 E^0
-1 ~ 0
•1 a 0
5 0
0
0 20 "-10 50 3 0 -100 1 E0 -1 L-10 "ISO 130 E00
TIME, days
Figure C.101. Chemical oxygen demand concentration of
influent synthetic sewage at Point B in
the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19» 1980.
50 0
5>-t0
*—iB 0
L-.E0 .
*	3E0
*	300
o i—'t—< 0
o
•1 B 0
1 E 0
E0
0
0 E0 *-(0 50 0 0 -10 0 '1 S0 '1 L-l© '15 0 '100 E00
TIME, days
Figure C.102. Chemical oxygen demand concentration of
effluent synthetic sewage from the reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
Start-up of the second unit was September 5,
1980.
h A
U83

-------
2Q Q
•1 BQ -¦
•1 E8 ¦¦
• m 0 - -
•1E0--
'100 ¦¦
B 0 - •
EQ --
t-,0
E0
0
/
A

/
/
/
""
\ /
V
v
0
-10
a© as 30
TIME, days
35 i-i0 L-i 5 50
Figure C.103.
E0 0
Chemical oxygen demand concentration of
effluent .synthetic sewage at Point A in the
second unit of the two unit reed canary
grass Cornell KFT in Phase 1. First day of
analysis was March 19, 1980. Start-up of
titer second unit was September 5, 1980.
1 B0 --
1 E0 --
1 L-10 --
1 E0 --
1 m 0 - -



-o
ei
-1 5
T3
E8 S5 30
TIME, days
35 Li 0 L-i 5 5fc.i
Figure C.104.
Chemical oxygen demand concentration of
effluent synthetic sewage at Point B in
the second unit of the two unit reed
canary grass Cornell NFT unit in Phase 1.
First day of analysis was March 19, 1980.
Start-up of the second unit was September 5,
1980.
U8U

-------
-1 B 0
1E0
1 1-10
•	1 a 0
•	1 0 0
B0
E 0
L-10
E 0
0
0 5 -10 "15 2 0 as 30 35 L-i 0 i-i 5 50
TIME, days
Figure C.105. Chemical oxygen demand concentration of
effluent synthetic sewage in the second
unit of the two unit reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980. Start-up
of the second unit was September 5, 1980.
P
/
U85

-------
EG 0
5L-I0
L-.BQ
I-.E0
3E0
30 0
ah©
iae
-1 2 a
5 0
0
0 50 L-i© E0 BQ '100 '1 Sp 'I *—10 '1 E0 1 B0 200
TIME, days
Figure C.106. Soluble chemical oxygen demand concentration
of influent synthetic sewage delivered to
the reed canary grass Cornell NFT in Phase 1
First day of analysis was March 19, 1980.
'—1 £3
!-iS0
35 £1
30 0
E3U0
1 a p|
150
¦f
i
r
>jj .'3 a '3
- 1^3 1.
$ \
i'a
\ |3-cpsi3
a	±
T3~
1 i-O ~
-a
33
lil
L-i0 50 Bb 1 0 hi 1c:a 1 *-10 ¦1E0 '1S0
TIME, days
!00
Figure C.10T. Soluble chemical oxygen demand concentration
of influent synthetic sewage at Point A in
the reed canary grass Cornell NFT in Phase 1
First day of analysis was March 19, 1980.
U86

-------
B0 0
S1-! 0 - -
l-iBS • -
^aa
3E0 --
30 0 - ¦
B1-! 0 -•
1 El 0 4
• 1 a 0
5 0
0


! ca«j q
bJ 'Ja
° as—_
3
& £50'
R
-—€) ? C
—j£_
0
J0
1-10 B0
B0 -1tJ0 1Et3 -1 l-i© -1E0 -1B0 E00
TIME, days
Figure C.108. Soluble chemical oxygen demand, concentration
of influent synthetic sewage at Point B in
the reed canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
S0 0
5I-.0
^0
^ L-|E 0
tjo
s 350
g" 30 0
o
o
M i a 0
1 50
E 0
0
01 E0 L-I0 E0 0 0 -10 0 't 20 '1 '—10 -150 1 B 0 S00
TIME, days
Figure C.109. Soluble chemical oxygen demand concentration
of effluent synthetic sewage from the reed
canary grass Cornell NFT in Phase 1. First
day of analysis was March 19, 1930.
US7

-------
50 0
-1 B 0
• 1 5 fcj
me
to
5 1 a q
g '10 0
o
J BQ
o
M 5 0
L-,0
5 0
0
0 5 10 '15 50 55 30 35 US >-.5 50
TIME, days
Figure C.110. Soluble chemical oxygen demand concentration
of effluent synthetic sewage at Point A in
the second unit of the two unit reed canary
grass Cornell KFT in Phase 1. First day of
analysis was March 19, 1980. Start-up of
the second unit was September 5, 1982.
50 0
¦10 0
-1 E 0
^ m 0
5sD
e -1 a 0
§ '10 0
O
^ B0
o
w 5Q
1-10
EQ
0
0 5 -10 15 5 0 5 5 30 35 h0 "iS 50
TIME, days
Figure C.lll. Soluble chemical oxygen demand concentration
of effluent synthetic sewage at Point B in
the second unit of the two unit reed canary
grass Cornell NFT in Phase 1. First day of
analysis was March 19, 1980. Start-up of
the second unit was September 5, 1980.
U88

-------
SQ0
•1 BQ -¦
=>< 1 E y - ¦
a -m0..
q" -1E0--
o
o _
-1 0 0 - ¦
J
°	r-, -
cn B 0 - -
B0
1-10
E0
0
0 5 -10 -15 20 25 30 35 h0 u.5 50
TIME, days
Figure C.112. Soluble chemical oxygen demand concentration
of effluent synthetic sewage in the second
unit of the two unit reed canary grass
Cornell RFT in Phase 1. First day of
analysis was March 19, 1980. Start-up of
the second unit was September 5, 1980.
U89
/

-------
h5
1—1 0
35
3 0
25
E 0
•1 5
-1 0
5
0
0 -15 30 i-i5 E0 75 50 -1 05 -1E0 -135 -150
TIME, days
Figure C.113. Total nitrogen concentration of influent
synthetic sewage delivered to the reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, I960.
(i "3.
K I
p--f3
\T\
EJ

""Y
[I fa
I
I	I	1	I	1	1	1	1	1-
M
•15 30
Figure C.llU,
L-i5 E 0 7 5 30 1 05 130 13 5 150
TIME, days
Total nitrogen concentration of influent
synthetic sewage at Point A in the reed
canary grass Cornell NFT in Phase 1. First
day of analysis was March 19, 1980.
1+90

-------
c
0 15 30
Figure C.115-
L-,5 E0 75 50 '1 05 -1S0 -135 150
TIME, days
Total nitrogen concentration of influent
synthetic sewage at Point B in the reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
Start-up of zhe second unit was September 5,
1980.
V X A
v* r~°
0
30 "-iS E0
75 30 -1 05 -1 20 13 5 1 50
TIME, days
Figure C.116. Total nitrogen concentration of effluent
synthetic sewage from the reed canary
grass Cornell NFT in Phase 1. First day
of analysis was March 19, 1980.
U91

-------
I-.5 --
>-10 --
* 50-
•15-
-1 0 - -
5 -¦
0 H	>	I	1	I	1	I	1	I	1
0 5 "10 -15 2 0 3 5 30 35 >-.0 >-.5 50
TIME, days
Figure C.117. Total nitrogen concentration of effluent
synthetic sewage at Point A in the second
unit of the two unit reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980. Start-up of
the second unit was September 5, 1980.
U92

-------
TIME, days
Total nitrogen concentration of effluent
synthetic sewage at Point B in the second
unit of the two unit reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 195 1980. Start-up
of the second unit was September 5, 1980.
U93

-------
5 0
no
a
s
Eh
0
-10 15
20 25 30
TIME, days
35 10 Li 5 50
Figure C.119.
Total nitrogen concentration of effluent
synthetic sewage in the second, unit of
two unit reed canary grass Cornell NFT
in Phase 1. First day of analysis was
March 19, 1980. Start-up of the second
unit was September 5, 1980.
U9U

-------
\ iW
0 15 3Q i-<5 B 0
75 30 -1 05 -1E0 -13 5 1 50
TIME, days
Figure C.120.
Soluble total nitrogen concentration of
influent synthetic sewage delivered to
the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980.
i-Q. Q-q	S.
^ ^	- 1 'b?	.a ft
/\
"4 \j ^

5 0
¦-i 5 -t-
1-10
35
3 0
35
3 0
1 5
-1 0 --
5 --
0 "15 30 1-.5 E0 7 5 30 -1 05 130 "I 35 1 50
TIME, days
Figure C.121. Soluble total nitrogen concentration of
influent synthetic sewage at Point A in
the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980.
^95

-------
ckl3 W-G)

0 IB 3& I-.5 B 0 75 30 105 150 135 150
TIME, days
Figure C.122.
Soluble total nitrogen concentration of
influent synthetic sewage at Point B in
the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980.
oj
—,
30-
60

y
as-
a
se --
0
15-



10-

5 -

0-
0
Figure C.123.
30 Li5 E0 75 30 10B 150 135 -150
TIME, days
Soluble total nitrogen concentration of
effluent synthetic sewage from the reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980,
U96

-------
5 0
tJD
a
a
Eh
~q
O
CO
50
Figure C.12U.
to
S
&
Eh
~J
O
cn
TIME, days
Soluble total nitrogen concentration of
effluent synthetic sewage at Point A in
the second unit of the two unit reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19» 1980.
Start-up of the second unit was September 5,
1980.
50
50
TIME, days
Figure C.125-
Soluble total nitrogen concentration of
effluent synthetic sewage at Point B in
the second unit of the two unit reed
canary grass Cornell NFT. First day of
analysis was March 19» 1980. Start-up of
the second unit was September 1980.
U97

-------
50
M5 --
t-,0 - .
35-.
: 25-

-1 0 -•
0
0 S 10 15 EG 55 30 35 ^0 hS 50
TIME, days
Figure C.126. 'Soluble total nitrogen concentration of
effluent synthetic sewage in the second
unit of the two unit reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980. Start-up
of the second unit was September 5, 1930-
U98

-------
50 T
L.5 --
L-tQ
^ 3B
s
. 30
23
r, E5 +
o
cc
o
E0-.
•1 5 -¦
•1 0 -•
0
Q--SJ
y
!4 t
a
•15 30 Lt5
5 0 75 30 'I 05 1
TIME, days
!0 -135 150
Figure C.127.
Organic nitrogen concentration of
influent synthetic sewage delivered
to the reed canary grass Cornell NFT
in Phase 1. First day of analysis was
March 19, 1980.

50 t

«i5 ¦¦

1—10 -¦


tin
35 --
a

r\
30 -¦
s

o
35 -•
M

3
C
30 ¦¦
O

o
•1 5 --

"10ii


5--

0 -¦

0
-15 30 i-i5
E 0 r" 5 50 -1 05 -1 3 0 13 5 '150
TIME, days
Figure C.128.
Organic nitrogen concentration of
influent synthetic sewage at Point A
in the reed canary grass Cornell NFT
in Phase 1. First day of analysis
was March 19, 1980.
1+99

-------
he
S
o
o
«
O

30 L-iS
Figure C.129.
E0 75 3%^ 05 -120 -135 '1 50
TIME, days
Organic nitrogen concentration of influent
synthetic sewage at Point B in the reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.


M
1 5 30 L-(5
BQ 75 30 -1 05 120 -13 5 -150
TIME, days
Figure C.130.
Organic nitrogen concentration of effluent
synthetic sewage from the reed canary
grass Cornell NFT in Phase 1. First
day of analysis was March 19, 1980.
500

-------
1 0
S
o
o
o
~
~ -•
7 --
B -¦
5 ¦¦
i-i -•
3 -¦
a ¦¦
[j
•1 --
0-
0
-1 0
20 SS 30 35
TIME, days
•-10 Li 5 5fcJ
Figure C.131.
Organic nitrogen concentration of effluent
synthetic sewage at Point A in the second
unit of the two unit reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980. Start-up
of the second unit was September 5, 1980.
s
o
M
o
o
15 50 as 30 35 «-.0
TIME, days
5 50
Figure C.132.
Organic nitrogen concentration of effluent
synthetic sewage at Point B in the second
unit of the two unit reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980. Start-up
of the second unit was September 5, 1980.
501

-------
1 0 y
3 --
B ¦¦
7--

0 5 10 15 50 55 30 35 L-.0 "-.5 50
TIME, days
Figure C.133. Organic nitrogen concentration of effluent
synthetic sewage in the second, unit of the
two unit reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980. Start-up of the second
unit was September 5, 1980.
502

-------

co •1 a
>-i5 E0 ?5 30 -1 05 120 135 150
TIME, days
Figure C.13^. Soluble organic nitrogen concentration
of influent synthetic sewage delivered
to the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980.

5 0 -r

I-.5 --
c*
1-10 -
to

1=1
35 --
3
30-
O

H
F3
35 --
<

u
re
2 0 --
o

j
•1 5 ¦¦
o

Ui
•1 0t,

5--

0 --
•15 30jy( «-.5 50 75 30 -1 05 -120 135 150
TIME, days
Figure C.135.
Soluble organic nitrogen concentration
of influent synthetic sewage at Point A
in the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980.
503

-------

50 75 30 -1 05 -1S0 135 -15Q
TIME, days
Figure C.136. Soluble organic nitrogen concentration
of influent synthetic sewage at Point B
in the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980.
iQ
I-.5 --
1-10 --
35 --
30-
35 --
3 0 --
•1 5
-1 0
A /I
^		
0 -15 30 L-i5 E0 7 5 30 1 OS 130 135 -150
TIME, days
Figure C.13T* Soluble organic nitrogen concentration
of effluent synthetic sewage from the
reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980.
50U

-------
Q
M
-10 -15
Figure C.138.
t	i
50 a5 30
TIME, days
35 10 m
50
Soluble organic nitrogen concentration
of effluent synthetic sewage at Point A
in the second unit of the two unit reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
Start-up of the second unit was September
1980.
M
A 0
•15 E0 55 30
TIME, days
35 L-.0 L-i 5 50
Figure C.139.
Soluble organic nitrogen concentration
of effluent synthetic sewage at Point B
in the second unit of the two unit reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
Start-up of the second unit was September 5
1980.
505

-------
'1 0y
a..
B -¦
0 5 'IQ -15 50 25 30 35 L-i0 •—15 50
TIME, days
Figure C.lUO. Soluble organic nitrogen concentration
of effluent synthetic sewage in the
second unit of the two unit reed canary
grass Cornell NFT in Phase 1. First
day of analysis was March 19, 1980.
506

-------
50
i_, 5
1_,0 ..
35--
a*
w 3 0 4-
\m
0 50 i-i0 B0
Figure C.lUl.
00 -100 1 50 -1 Li© 1E0 1By 500
TIME, days
Ammonia nitrogen concentration of
influent synthetic sewage delivered
to the reed canary grass Cornell TIFT
in Phase 1. First day of analysis
was March 19, 1980.
to
6
-3"
S3
25
1-.0--
0 50
Figure C.1U2.
_t	
50 B0 -100 -1 50 1 M0 1E0 '1 B0 50©
TIME, days
Ammonia nitrogen concentration of
influent synthetic sewage at Point A
in the reed canary grass Cornell NFT
in Phase 1. First day of analysis
was March 19, 1900.
507

-------
vs.
30

50-
20 i-i0 B0
»	t	
B0 -100 -130 1i-r0 *1 B0 -1B0 200
TIME, days
Figure C.1U3.
Ammonia nitrogen concentration of
influent synthetic sewage at Point B
in the reed canary grass Cornell NFT
in Phase 1. First day of analysis
was March 19, 1980,
50
'-i a - •
H0--
35 --
30 4
ftp
A
t hi


20 --
1 S--
¦ 1 0 - -
/

0
H—F3	(-
kil 20 1-10
Figure C.lUU,
5u B 0 '100 -120 1 Ma ¦ 1 E 0 '100 200
TIME, days
Ammonia nitrogen concentration of
effluent synthetic sewage from the reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 19SO.
508

-------
1-10
aat a
Figure C.1U5.
32 -1-1 5 -13B -1 B-1 -1 B1-! 207 E3Q
TIME, days
Ammonia nitrogen concentration of
effluent synthetic sewage at Point A
in the second unit of the two unit
reed canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
Start-up of the second unit was September
1980.
h0
fl

4
0 23 i-iE E3 3 2 1 -1 5 -1 3B -1 E1 10^ 207 230
Figure C.1U6.
TIME, days
Ammonia nitrogen concentration of
effluent synthetic sewage at Point B
in the second unit of the two unit reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
Start-up of the second unit was September 5
1980.
509

-------
35 -•
35 --
'1 B ¦¦
-1 a --
l-i -¦
0 23 l-iE E3 3Bj1j1_5 13B -1 E-1 1BI-. E0? S30
TIME, days
Figure C.lUT. Ammonia nitrogen concentration of
effluent synthetic sewage in the second
unit of the two unit reed canary grass
Cornell UFT in Phase 1. First day of
analysis was March 19, 1980. Start-up
of the second unit was September 5, 1990.
510

-------
50
;r V, U*
0 20 L-i0 B0 B0 -100 'I 2tl -1 L-i0 'IE© -1 Bk) S00
TIME, days
Figure C.1U8. Soluble ammonia nitrogen concentration
of influent synthetic sewage delivered
to the reed canary grass Cornell NFT
in Phase 1. First day of analysis was
March 19, 1980.
L
0 Bfc) L-it) EfcJ
B0 -100 '1E0 -1 '—10 1 B0 -1B0
TIME, days
WW
Figure C.1U9.
Soluble ammonia nitrogen concentration
of influent synthetic sewage at Point A
in the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980.
511

-------
50
30 II fei

J
Figure C.150.
-i	1	1	1-
3 0 -100 ¦I E0 -1 •—»0 1 E0 '130 200
TIME, days
Soluble ammonia nitrogen concentration
of influent synthetic sewage at Point A
in the reed canary grass Cornell NFT
in Phase 1. First day of analysis was
March 19> 1980.
5 0
L-lE -¦
L-t 0 - -
35.
b0
s 3 0
S 25--
K
3
03
: W ...
-1 5 • ¦
1 G +
* \ }
\!
U 20 '-.t:
Eh 1 ~ jm | i_i i '-im | 5y 'l Bb 2-oi-j
TIME, days
Figure C.151. Soluble ammonia nitrogen concentration
of effluent synthetic sewage from the
reed canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
512

-------
/ I ^ w
h—a
kJ
S3 i-E E3
3S -11 5 -13B -1 E-1 -IB1-! S07 E30
TIME, days
Figure C.152.
Soluble ammonia nitrogen concentration
of effluent synthetic sewage at Point A
in the second unit of the two unit reed
canary grass Cornell KFT in Phase 1.
First day of analysis was March 19, 1980.
L-.0
31-.--
3-1
SB-
s
i
+
Q	L-iE 53 3 3 1-1 5 1 3B 1 E1 -IB1-! 207 E30
TIME, days
Figure C.153. Soluble ammonia nitrogen concentration
of effluent synthetic sewage at Point B
in the second unit of the two unit reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
513

-------
r\r~
T
8 S3 1-1E E3
ga 115 13B -1E-1 1BhS0? S30
TIME, days
Figure C.15U.
Soluble ammonia nitrogen concentration
of effluent synthetic sewage in the
second unit of the two unit reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
Start-up of the second unit was September 5,
1980.
5lU

-------
60
s
a
i
on
0 S
Figure C.155-
•1 0 -1 S 1 l-i
TIME, days
Nitrate nitrogen concentration of
influent synthetic sewage delivered
to the reed canary grass Cornell NFT
in Phase 1. First day of analysis was
May 13 , 1930.
h0
C5
z
I
cn
o
a
0 E
a -1 0 -1 a
TIME, days
IL-, -1
-1 a E0
Figure C.156.
Nitrate nitrogen concentration of
influent synthetic sewage at Point A
in the reed canary grass Cornell NFT
in Phase 1. First day of analysis was
May 13, 1980.
515

-------
•10 'IS
TIME, days
50
Figure C.157-
Nitrate nitrogen concentration of
influent synthetic sewage at Point B
in the reed canary grass Cornell TIFT
in Phase 1. First day of analysis was
May 13, 1980.
TIME, days
Figure C.158.
50
Nitrate nitrogen concentration of
effluent synthetic sewage from the reed
canary grass Cornell NET in Phase 1.
First day of analysis was May 13, 1980.
516

-------
0 E
L-,
Figure C.159.
• 1 0 1 E
TIME, days
•1L-,
•1 E
-1 3 EG
Soluble nitrate nitrogen concentration
of influent synthetic sewage delivered
to the reed canary grass Cornell NFT
in Phase 1. First day of analysis was
May 13, I960.
Figure C.lbO.
1 0 "1 E
TIME, days
•11-. 1 S 1 E
EG
Soluble nitrate nitrogen concentration
of influent synthetic sewage at Point A
in the reed canary grass Cornell NFT
in Phase 1. First day of analysis was
May 13, 1980.
517

-------

1 0

3

B


00
7
a


G
s

1
B
m

0
s
I-.
~J
0
a
CD


a

-1
0 a 1-1
Figure C.l6l.
b 1 0 _ -i a m
TIME, days
•1 E -1 B 20
Soluble nitrate nitrogen concentration
of influent synthetic sewage at Point B
in the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
May 13, 1980.

'1 0

3

B
(JO

g
T7
«s

3
I
B
on
0
B
3

hJ

0

CO
3

a

•1
0
10 1a -m
TIME, days
-1 B -1 B B0
Figure C.162.
Soluble nitrate nitrogen concentration
of effluent synthetic sewage from the
reed canary grass Cornell NFT in Phase 1.
First day of analysis was May 13, 1980.
518

-------
EG
-1 B
•1 E
1 i-.
-1 E
-1 Q
a
E
s
Q
Q EE S0 75 -10 0 -1E5 -1S0 -1 7S E00 EES E50
TIME, days
Figure C.163. Orthophosphate phosphorus concentration
of influent synthetic sewage delivered
to the reed canary grass Cornell NFT
in Phase 1. First day of analysis was
March 19» 1980.
E 0
•1 B
•1 E
•1
•1 E
•1 0
B
E
Li
E

ft fl

¦i/V
IP 19
a 4'
x4
rp 11
wf'9

a
05

•323
I	t	I	I	t	I
I	I
0 EE E0
Figure C.16U.
75 -10 0 -1 ES '1 A 75 Sidtj EES E5tJ
TIME, days
Orthophosphate phosphorus concentration
of influent synthetic sewage at Point A
in the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980.
519

-------
E0
•1 B
•1 5
•1
•1 a
1 0
B
E
>-i
E
0
0 E5 50 75 -100 1E5 '150 -1 75 200 2E5 E50
TIME, days
Figure C.165. Orthophosphate phosphorus concentration
of influent synthetic sewage at Point B
in the reed canary grass Cornell NTT in
Phase 1. First day of analysis was
March 19, 1980.
E0
1 B
-1 B
•1 l-.
•I E
•1 0
B
E
E
0
0 E5 50 75 100 -12 5 150 -1 75 500 55 5 550
TIME, days
Figure C.166. Orthophosphate phosphorus concentration
of effluent synthetic sewage from the
reed canary grass Cornell NFT in Phase 1
First day of analysis was March 19, 1980
- R

9 Ftp
i ^ j 'i
\ I *


i%]
a^
t h.
\ fi
,U I Q
1/
H	1	1-
H	1	I
520

-------
E0 y
-1 B--
1 E ¦¦
0	I	I	I	1	1	I	1	t	t	
0 5-10-15 50 55 30 35 >-.0	50
TIME, days
Figure C.167. Orthophosphate phosphorus concentration
of effluent synthetic sewage at Point A
in the second unit of the two unit reed
canary grass Cornell NFT in Phase 1. First
day of analysis was March 19, 1980. Start-
up of the second unit was September 5, 1980.
0
•1 0 -1 5
50 55 30
TIME, days
35 L-i 0 L-i 5 50
Figure C.168. Orthophosphate phosphorus concentration
of effluent synthetic sewage at Point B
in the second unit of the two unit reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
Start-up of the second unit was September 5,
1980.
521

-------
r
0
-10 is a
0 B5 30 35
TIME, days
•-10 '-i 5 50
Figure C.169.
Orthophosphate phosphorus concentration
of effluent synthetic sewage in the
second unit of the two unit reed canary
grass Cornell NFT in Phase 1. Firsr day
of analysis was March 19, 1980. Start-up
of the second unit was September 5, 1980.
0 25 50 75 1vj0 125 150 i 75 20 tJ 2d5 25ti
TIME, days
Figure C.1T0. Soluble orthophosphate phosphorus con-
centration of influent synthetic sewage
delivered to the reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980.
522

-------
ee
R rt„ \
8 E5 50
Figure C.1T1.
75 -10 0 -1 55 -150 -1 75 E00 EE5 E50
TIME, days
Soluble orthophosphate phosphorus con-
centration of influent synthetic sewage
at Point A in the reed canary grass
Cornell NFT unit in Phase 1. First day
of analysis was March 19, 1980.
c i tn
n
/
0 E5
50
Figure C.172.
75 -10 0 -155 1 50 1 75 200 EE5 E50
TIME, days
Soluble orthophosphate phosphorus con-
centration of influent synthetic sewage
at Point B in the reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980.
523

-------
20
-1 a
1	E
1	L-.
-1	a
•1	0
B
E
L-.
a
0
Mir
\
b a
f
A
0 EE 50
Figure C.1T3.
75 -100 -1E5 -150 -1 75 200 225 250
TIME, days
Soluble orthophosphate phosphorus con-
centration of effluent synthetic sewage
from the reed canary grass Cornell NFT
in Phase 1. First day of analysis was
March 19 > 1980.
M
•10 -15 20 25 30 35 U0
TIME, days
50
Figure C.IT^.
Soluble orthophosphate phosphorus con-
centration of effluent synthetic sewage
at Point A in the second unit of the
two unit reed canary grass Cornell NFT
in Phase 1. First day of analysis was
March 19, 1980. Start-up of the second
unit was September 5, 1980.
52U

-------
EG
-1 a..
•1 E -¦
•1 L-.
-1 a
•1 0
B
E
i-i
2
0
0 5 10 -15 20 55 30 35 >-.0 L-.5 50
TIME, days
Figure C.175. Soluble orthophosphate phosphorus con-
centration of effluent synthetic sewage
at Point B in the second unit of the
two unit reed canary grass Cornell NFT
in Phase 1. First day of analysis was
March 19, I960. Start-up of the second
unit was September 5, 1980.
2 0
1 B
-1 E
•1 l-.
1 E
-1 0
B
5
i-.
E
0
0 5 -10 -15 S0 E5 30 35 >-.0 >-i5 50
TIME, days
Figure C.176. Soluble orthophosphate phosphorus concen-
tration of effluent synthetic sewage in
the second unit of the two unit reed
canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
Start-up of the second unit was September 5,
1980.
/
A
0—	B-	Q
-I- \
1 V
/
/ \
/ k"
till	1	1	t	t
525

-------
-
IB S >-i 3 0 3B
TIME, days
>-i5 1-.B 5i-i E0
Figure C.177-
Cadmium concentration of influent synthetic
sewage delivered to the reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980. Start-up
of the second unit was September 5, 1980.
-1
0 iB0
0 o B 0
0.70
0 a B 0
IP 0.50
rtf" 0 B I 0
o
0=30
0 ~ 2 0
0,10
0
0 B -15 1B Si-. 30 3E L-.E >-tB 5<-. S0
TIME, days
Figure C.1T8. Cadmium concentration of influent synthetic
sewage at Point A in the reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980.
526

-------
•1
0 .30
0 aB0
0o?0
cj 0 ~ E 0
S 0 = 50
0 »h0
o
0 D 3 0
0=50
0.-10
0
0 E -15 'IB 51-. 30 3E I-.E hB 5"-. E0
TIME, days
Figure C.179. Cadmium concentration of influent synthetic
sewage at Point B in the reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980.
1
0 a 3 0
0=30
0a?0
oi 0 n E 0
g 0-50
Tj 0 a *-1 0
O
0=30
0 a 3 0
0='10
0
0 e ia -1 b a>-. 30 3E i-.a >-.b 5>-. e©
TIME, days
Figure C.180. Cadmium concentration of effluent synthetic
sewage from the reed canary grass Cornell
NFT in Phase 1. First day of analysis
was March 19» 1980.
527

-------
Q ~ Li 5
3^0
Q .35
0-30
0.S5
0 .20
0.1 5
0 a'l 0
0 .05
0
0 15 30 I-.5 50 75 30 -1 05 120 135 150
TIME, days
Figure C.l8l. Cadmium concentration of effluent synthetic
sewage at Point A in the second unit of the
two unit reed canary grass Cornell NFT in
Phase 1. First day of analysis was March 19
1980. Start-up of the second unit was
September 5, 1980.
y o50
0 .1-15
0 D I 0
0 d35
0.30
0 .35
0 oE0
0 = 15
0 . 1 0
0.05
0
0 -15 30 L.5 G0 7 5 30 1 05 1S0 -135 150
TIME, days
Figure C.182. Cadmium concentration of effluent synthetic
sewage at Point B in the second unit of the
two unit reed canary grass Cornell NFT in
Phase 1. First day of analysis was March 19
1980. Start-up of the second unit was
September 5S 1980.
\
528

-------
0 = 50
0 .>-.5 ..
0 a L-l 0 - ¦
0.35..
M)
S
0 .30 -¦
„ 0 oE5 -¦
° 0.50-
0.15-
0c1 fe) ¦ ¦
0 .05-
£U^Sr^.

0
0 -15 30 L.5 B0 75 30 1 05 -130 135 -150
TIME, days
Figure C.183. Cadmium concentration of effluent
synthetic sewage in the second unit
of the two unit reed canary grass
Cornell NFT in Phase 1. First day
of analysis was March 19, 1980.
Start-up of the second unit was
September 5, 1980.
529

-------
a
p,
0 20 1-10 50 B0 -100 '1 E0 1 t-10 -1B0 '1B0 E00
TIME, days
Figure C.18U. pH of influent synthetic sewage
delivered to the reed canary grass
Cornell NFT -in Phase 1. First day
of analysis was March 19, 1980.
•1 0 f
•1 id f
3 L
3 1
w _
P< a

. A'
-a

;u :
j i >i' i£i
1 tl •-!:
TIME, days
Figure C.I85.
pH of influent synthetic sewage at
Point A in the reed canary grass
Cornell NFT in Phase 1. First day
of analysis was March 19, 1980.
530

-------
as
p<
		^ -3
r
^	1_
dQ ^0
Figure C.186.
	1	i	1	1	!	H	1	!
E0 B0 ;1 01-9 1 E0 I L-i0 'i EG 1 5 0 300
TIME, days
pH of influent synthetic, sewage at
Point B in the reed canary grass
Cornell NFT in Phase 1. First day
of analysis was March 19, 1980.
I I ' T
El
a
% a
4-
1
r
13*
,--3 ?
a




a-3""
iiu'

3 -1	1	!	1	1	1	1	1	1	1	1
i.-j 30 i-ifci Bid Bid '1 fe.1 W -'1 i LifcJ • 1 fca y -I 2 U 3idid
TIME, days
Figure C.I87. pH of effluent synthetic sewage from
the reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980.
531

-------
1 0
sc
ft
u
•10 -15 S0 35 30 35 1-10 I-.5 50
Figure C.188.
TIME, days
pH of effluent synthetic sewage at
Point A in the second unit of the
two unit reed canary grass Cornell
NFT in Phase 1. First day of
analysis was March 19, 1980. Start-up
of the second unit was September 5, 1980.
1 0 j-
3 -¦
5 --
K 5 -¦
ft
l-l..
3 ¦¦
3 -•
•1 --
0 -I		1 I	1	
0 5 10 15 30 35 30 35 L-.0 i-i5 50
TIME, days
Figure C.I89. pH of effluent synthetic sewage at
Point 3 in the second unit of the two
unit reed canary grass Cornell NFT in
Phase 1. First day of analysis was
March 19, 1980. Start-up of the second
unit was September 5, 1980.
532

-------
H	1	I	I	1	<	1	1	1	
0 5 10 15 50 55 30 35 "-.0 I-.5 50
TIME, days
igure C.190. pH of effluent synthetic sewage in.the
second unit of the two unit reed canary
grass Cornell NFT in Phase 1. First
day of analysis was March 19, 1980.
Start-up of the second unit was
September 5 , 1980.
533

-------
y ffcJV	^
'K pa	JmJ
\ '
a'
Q EQ '—10 E0 00 -1Q0 -1 EG 1 i-iQ "1S0 1B0 S00
TIME, days
Figure C.191. Soluble pH of influent synthetic sewage
delivered to the reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980.
'1 0
3
a
7
E
5
0 E0 L-10 E0 Ski 1 yu 1 Ey 1 *—-1 bay 1 By Efti
TIME, days
Figure C.192. Soluble pH of influent synthetic sewage
at Point A in the reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980.
ci S -J3--
---a
53H

-------
0 50 L.0 B0 00 '100 "120 1 L-.0 -1E0 -1B0 200
TIME, days
Figure C.193. Soluble pH of influent synthetic sewage
at Point B in the reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980.
1 0
t	I	H
0 20 L_,0 E0 B0 100 -120 1 I-.0 1 B0 1B0 200
TIME, days
Figure C.19U. Soluble pH of effluent synthetic
sewage from the reed canary grass
Cornell NFT in Phase 1. First day
of analysis was March 19, 1980.
535

-------
0 t	I	I	t	t	t	t	t	t	t
0 5 '1Q -IB 59 25 30 35 >-.0 ^5 50
TIME, days
Figure C.195. Soluble pH of effluent synthetic sewage
at Point A in the second unit of the
two unit reed canary grass Cornell NFT
in Phase 1. First day of analysis was
March 19, 1980. Start-up of the second
unit was September 5, 1980.
1 0
~
B
"?
E
5
i-i
3
-1
0
0 5 10 -15 20 25 30 35 ^0 1-.5 50
TIME, days
Figure C.196. Soluble pH of effluent synthetic sewage
at Point B in the second unit of the
two unit reed canary grass Cornell NFT
in Phase 1. First day of analysis was
March 19, 1980. Start-up of the second
unit was September 5, 1980.
536

-------
SC
ft
iJ
O
za
0 5 -10 -15 E0 E5 30 35 I-.0 h5 50
TIME, days
Figure C.197. Soluble pH of effluent synthetic sewage
in the second unit of the two unit
reed canary grass Cornell NFT in Phase 1.
First day of analysis was March 19, 1980.
Start-up of the second unit was September 5,
1980.
5 37

-------
H	1	t	I	I	I	1	I	1	1
0 E "12 1B E"-. 3 0 3E ^a >-iB 5^ E9
TIME, days
Figure C.198. Zinc concentration of influent synthetic
sewage delivered to the reed canary
grass Cornell NFT in Phase 1. First
day of analysis was March 19» 1980.
E
E
a
•1
-1
0-1 0 0
0 ~ B O
kJ ~ E 0
0.^0
0 =50
0
0 E 12 -1B EE *-1 3 0 3E 1-.E 1-.B E"-. E0
TIME, days
Figure C.199- Zinc concentration of influent synthetic
sewage at Point A in the reed canary
grass Cornell NFT in Phase 1. First day
of analysis was March 19, 1980.
538

-------
00-
a«-t 30 3E
TIME, days
EG
Figure C.200.
Zinc concentration of influent synthetic
sewage at Point B in the reed canary grass
Cornell NFT in Phase 1. First day of
analysis was March 19, 1980.
E
a
a
1
•Si i
60
S 0-100
*
tS 0 n B 0
t) nEt)
0 ~'-10
0 a E 0
0
0 5 -ta -1 s a>-t 30 3E i-.a ha s>-. E0
TIME, days
Figure C.201. Zinc concentration of effluent synthetic
sewage from the reed canary grass Cornell
NFT in Phase 1. First day of analysis
was March 19, 1980.
539

-------
TIME, days
Figure C.202. Chemical oxygen demand concentration of influent
synthetic sewage to the first unit of the three
unit Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
-j	
5-0 j
!—i 3 fci - -
h5 0 •-
3E0 • •
20 0 --
i
5<-10 (-
^ •
1 30
1 a 0 +
a i, 5
LH53 A
hi
S0-1-
ttr
i
..-3
I 'V '3
i|
~
"r
!i,
sit /
' i \ *
ra ;
iS
I

iiS 23 i£l tateJ 3y I ' 'Jtl 1 EHsii.s -1 ii£l 1 1 3y eSte.1t1
TIME, days
Figure C.203. Chemical oxygen demand concentration of effluent
synthetic sewage in Phase 1. First day of
analysis was February 16, 1981.
5^0

-------
60
E
P
O
u
5y m
L-( 5 0 • ¦ -
L- 0 0 - -
350 --
30 9 -
E50 --
13
E0 0 -r
I
1 5 0 - J,
•1 0 0 --
/ ''
! id

o__
=e|
0

-1 -1
c3—
-!S2S£EB6S-Ha-
33

15 b:Ld
r t*
SB 3 3-1 -1 0
Figure C.204.
TIME, days
Chemical oxygen demand concentration of influent
synthetic sewage to the second unit of the three
unit Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
oo
E
O
o
u
50 0
S1-! 0 -¦
<-iB0 --
<-.3 0 -
35 0 --
30 0 --
Eii 0 - -
1 B0ii
1.2 0
5 0
0
0


A

l a
f ."J
t
a.
fl
I

-0 '-id Eu Su 1 fcj 0 '1 30 '1 L,0 150 1B0 300
TIME, days
Figure C.205. Chemical oxygen demand concentration of effluent
synthetic sewage from the second unit of the three
unit Cornell NFT in Phase 1. First day of
analysis was February 16, 1981.
>hi

-------
B00
'-.~0
>-iE Q
350 -¦
30 0 -¦
a^0-
1 B0 -¦
-1 E0 • ¦
50 i
M
5?
E0 1-10 E0 00 -100 -1EQ -1 L-i0 -1E0 1 30 E00
Figure C.206.
TIME, days
Chemical oxygen demand concentration of effluent
synthetic sewage from the third unit of the three
unit Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.

to
e
a
o
u
o
CO
'."J
i. i
f [r
— —d
I50i
L*i
= i-n
r
I—
cr'J L10 St1 Ew	*'	1i b "Li '! "• S':J0
Figure C.207.
TIME, days
Soluble chemical oxygen demand concentration of
influent synthetic sewage to the first unit of
the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
5^2

-------
k_1
b'-iy
—i El ki*
'-,5 0
350
30 0
em3
•1 3 0 4
.¦¦) 3 0 .||	—
E0
CP
"1 /1
i? Lg
9
f/l
,' i2 i
P.
/
tp
1
&
-4-
-+-
_12L
. W
\/
ill
0
/ t
iS a
'"ffl
-+-
-H
-+-
•d 5y 1-iQ E0 B0 -1 0W -1 2t) -1 •—10 1 S0 -'130 200
Figure C.208.
TIME, days
Soluble chemical oxygen demand concentration of
effluent synthetic sewage from the first unit of
the three unit Cornell NFT system in Phase 1,
First day of analysis was February 16, 1981,


S0 Jj
1
2~>,Z3
ig3B»-+THi—h
L_, L.
I=:
lis 2 -'1 1'
TIME, days
Figure C.209. Soluble chemical oxygen demand concentration of
influent synthetic sewage to the second unit of
the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
5^3

-------
EG 0
b^U
¦-.5 0
L,5 0
35 0
308
EL,0
1 B0
120
A P?! v R,<
^ \/\ ^ A
0 20 >-10 E0 B0 -100 -1S0 1 l-(0 -1 50 -1 BfcJ 5a0
TIME, days
Figure C.210.
Soluble chemical oxygen demand concentration of
effluent synthetic sewage from the second unit
in the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
By 0
5*-i0 -¦
L-.B0 --
LiE0 --
35 0 -¦
30 0 -J-
ai-i0
1 B 0 - -
-1 5 0 --
0
f'
I / l!3
*0%
a
K

-? & V-g1^


y,
M
h—a—h£-
0 20 1-10
50 B0 100 120 "1 L-10 '150 -1B0
TIME, days

Figure C.211. Soluble chemical oxygen demand concentration of
effluent synthetic sewage from the third unit in
the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.

-------
5 0

3L-. ¦¦

30-


ftfl
EE-
s

#>
22 --
2

H
1 B -¦

-1 "-I --

-1 0 --

0
^ U Ja"1
3-1 -1 QL-i -1 -1 r -1 30
TIME, days
Figure C.212.
Total nitrogen concentration of influent
synthetic sewage to the first unit of the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 16, 1981.
5Q
¦-i 5
L-.0
35
^ 30
6 25
£ 20
•1 5
1 0
5
0
0 13 2E 33 52 E5 ?B 3-1 -10m-1? 130
TIME, days
Figure C.213. Total nitrogen concentration of effluent
synthetic sewage from the first unit in the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
5^5

-------
TIME, days
Figure C.214.
Total nitrogen concentration of influent synthetic
sewage to the second unit in the three unit
Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
50		
L-.0
35
301
55
^ E0
eo
6 1 5
i -is
5
0
0 -13 EE 33 55 E5 7B 5-1 10-1-1 7 130
TIME, days
Figure C.215. Total nitrogen concentration of effluent
synthetic sewage from the second unit in the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.
5 b6



-------
50
5 •-
0	t	i	1	i	1	i	1	1	1
0 -13 SE 33 53 E5 7B 31 -10H 7 -130
TIME, days
Figure C.216. Total nitrogen concentration of effluent
synthetic sewage from the third unit of the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
oo
S
o
LA
0
-13 EE
33 53 G 5
TIME, days
7B 3-1 1 01—. "1 "1 7 1 30
Figure C.217. Soluble total nitrogen concentration of
influent synthetic sewage to the first unit
of the three unit Cornell NFT system in Phase
1. First day of analysis was February 16, 1981.
5^7

-------
50	
<-.5
l-i 0
35
30i
55
50
-1 5
•1 0
5
0
0 -13 55 33 55 E5 7B 3-1 10L-. A 1 7 -1 30
TIME, days
Figure C.218. Soluble total nitrogen concentration of effluent
synthetic sewage from the first unit of the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.

50

55 33
BE 77 35 33 1-10
TIME, days
Figure C.219.
Soluble total nitrogen concentration of influent
synthetic sewage to the second unit of the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 16, 1981.
5U8

-------
50
1—10
^ 35
6
. 30
Z1
H 55
I 20
•1 5
•1 0
5
0
0 13 EE 33 5E E5 7B 3-1 10i-i ^17 '130
TIME, days
Figure C.220. Soluble total nitrogen concentration of effluent
synthetic sewage from the second unit of the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.
0
•1 3 EE 33
E 5 7 B 3-1 -1 0"-. 1 1 r 1 30
TIME, days
Figure C.221.
Soluble nitrogen concentration of effluent
synthetic sewage from the second unit of the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.
5^9

-------

ft

a
R,
'D1
/ ^
fij
a
J if
.1
\ {
0 -1B 3B S1-!
th
30 "10B -1 SB -1 "-.i-. -1EE 1E0
Figure C.222.
TIME, days
Organic nitrogen concentration of influent
synthetic sewage to the first unit of the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 16, 1981.
3B --
32 ¦¦
EB
^ Si-.-
60
6 E0--
*
f 1 B --
c_>
M
% "1 E ¦"
o
§ B
T
~ 5s!
y\ *
/ k
i

I
\	P
* i1 -«d"
0 -1B 3B B1-! 7 3 3 0 '10B -1 EB	-IBS 1B0
TIME, days
Figure C.223.
Organic nitrogen concentration of effluent
synthetic sewage from the first unit of the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
550

-------
TIME, days
Figure C.224. Organic nitrogen concentration of influent
synthetic sewage to the second unit of the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
E 0		
1 B
1 E
•1 l-i
•1 2
1 0
B
E
L-.I
a
0
0 'I? 3>-i E-1 EB BE -103 -1 -13 -13E -153 -170
TIME, days
Figure C.225. Organic nitrogen concentration of effluent
synthetic sewage from the second unit of the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981

551

-------
5 0 j-
1 B
1 E
•1 i-. --

0 -1B 3E 51-. ?S 30 1 0B -1 BE "1!—11—i 1E5 '1 S0
TIME, days
Figure C.226. Organic nitrogen concentration of effluent
synthetic Sewage from the third unit of the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
E Q
L-iB--

00
6 3B
2= 30-
u
T !? q^.
*11 ¦
V

El-,.. 
-------
*—I 0 T"
3E--
35-
ES --

0 -1 B 3E B1-! 72 30 -1 0B -1 EE mh 1BE -1 B0
TIME, days
Figure C.228. Soluble organic nitrogen concentration of
effluent synthetic sewage from the first unit
in the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
TIME, days
Figure C.229. Soluble organic nitrogen concentration of
influent synthetic sewage to the second unit
of the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
553

-------
OC
o
0
01
o
>J
o
w
0 -IB 3B 51-1 7i
30 -1 03 "1 BE -1-1 E2 -1 B0
TIME, days
Figure C.230.
Soluble organic nitrogen concentration of
effluent synthetic sewage from the second unit
in the three unit Cornell NTT system in Phase 1,
First day of analysis was February 16, 1981.
60
B
55
I
U
M
z
<
a
as
o
>J
o

0 -1B 3B
TIME, days
4	1 '-T3-H
30 "10B -1 SB -mi-t 1BE -1B0
Figure C.231.
Soluble organic nitrogen concentration of
effluent synthetic sewage from the third unit
of the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981,
55^

-------
00
a
z
i
ro
0 -13 SB 33 SB E5
TIME, days
7B 3-1 101-. -1-1 7 -130
Figure C.232. Ammonia nitrogen concentration of influent
synthetic sewage to the first unit of the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 16, 1981.
00
6
Z
I
X
z
I 0
354-
33
SB
Ei-.
E 0
•1 B
•1 a
-¦ <¥ fVsj
n B
V

I
I \
V
'¦!
a
r\
di
Lp.
\J W
TIME, days
Figure C.233. Ammonia nitrogen concentration of effluent
synthetic sewage from the first unit of the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
555

-------
1
)	t
1-1 SB 33	55 SB 77 BB 33 HQ
TIME, days
Figure C.234.
Ammonia nitrogen concentration of influent
synthetic sewage to the second unit in the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 16, 1981.
TIME, days
Figure C.235. Ammonia nitrogen concentration of effluent
synthetic sewage from the second unit of the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
556

-------
1-10
0 13 EE 33 55 E5 7B 3-1 -10l, -1 -1 7 -130
TIME, days
Figure C.236. Ammonia nitrogen concentration of effluent
synthetic sewage from the third unit of the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
E0
-1 B
•1 E
aT s
z
~J 5
S	1
a
0
0 13 EE 33 5 3 E5 7B 3-1 10^-1 17 130
TIME, days
Figure C.237. Soluble ammonia nitrogen concentration of
influent synthetic sewage to the first unit in
the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
557

-------
(10
s
z
I
ci
re
z
eJ
o

0 13 SB 33 5 a E5 73
TIME, days
3-1 -1 0L-, -1 1 7 -1 30
Figure C.238. Soluble ammonia nitrogen concentration of
effluent synthetic sewage from the first unit
in the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
u::
i
I
~ i ;
1? —If
t	I :
^ 1-|!
Z	' i
a I
° s
W	H
)
\
f .... i	i ¦— ¦>	—-f—	!•	'
•IH I-! £2 23 1—ii 55 b^c: r~? S3 S3 1-1 id
TIME, days
Figure C.239. Soluble ammonia nitrogen concentration of
influent synthetic sewage to the second unit
in the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
556

-------
L-iG
2B- ft/
\ 0 bj
TIME, days
Figure C.240. Soluble ammonia nitrogen concentration of
effluent synthetic sewage from the second unit
in the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
L-10
3 E
3S
20
EU
60
6
.R /
..ff a
20 -¦
f IB
CO
•1 a
o a
to

Ps-3
¦' b-
t/\J
/ fis
J
^ I1
TIME, days
Figure C.241.
Soluble ammonia nitrogen concentration of
effluent synthetic sewage from the third unit
in the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
559

-------
30
E 7
2L-.
2	1
•1 B
•1 5
-1 E
I
3
E
3
0
0 -13 SB 33 55 E5 7B 3-1 10^ M? -130
TIME, days
Figure C.242. Orthophosphate concentration of influent
synthetic sewage to the first unit of the three
unit Cornell NTT system in Phase 1. First day
of analysis was February 16, 1981.
3 0-i	
27 ..
2Li-
••'I
15';i4 —__ ~	.
H /V1'
3 -	^ \	j *
r-	Q	/
\ ;;
0 -I	* 1	> 1	1 1	1 1 >	
0 13 E5 33 5E ES 7B 31 -10 117 130
TIME, days
Figure C.243. Orthophosphate concentration of effluent
synthetic sewage from the first unit of the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
-¦ J
\
~
\ A
G? pi \
Q	I.
H


560

-------
"
~ ~ -1 '1 0
TIME,, days
Figure C.244,
Orthophosphate concentration of influent
synthetic sewage to the second unit in the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 16, 1981.
3 0
TIME, days
Figure C.245. Orthophosphate concentration of effluent
synthetic sewage from the second unit in the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.
561

-------
i ^'V
Q -13 EE 33 S3 55 7B
TIME, days
3-1 -10L, -M 7 130
Figure C.246. Orthophosphate concentration of effluent
synthetic sewage from the third unit in the
three unit Cornell NTT system in Phase 1.
First day of analysis was February 16, 1981.
30
33 5 3 5 5
TIME, days
1 QL-. -M 7 -130
Figure C.247.
Soluble orthophosphate concentration of influent
synthetic sewage to the first unit in the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 16, 1981.
562

-------
3 0
0 13 EE 33 SE E5
TIME, days
7B 3-1 -1 Q"-, -1 -1 ? 1 30
Figure C.248. Soluble orthophosphate concentration of
effluent synthetic sewage from the first unit
in the three unit Cornell NTT system in Phase 1
First day of analysis was February 16, 1981.
3 3 1 -1 0
TIME, days
Figure C.249.
Soluble orthophosphate concentration of
influent synthetic sewage to the second unit
in the three unit Cornell NFT system in Phase 1
First day of analysis was February 16, 1981.
563

-------
3 0
1 5 M

' 'h $
3-1 -IQ1-! 1 -1 7 -1 30
•13 EE 33 S3 E5
TIME, days
Figure C.250. Soluble orthophosphate concentration of effluent
synthetic sewage from the second unit in the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.
30 n

33 52 E5
TIME, days
3-1 1 0
-------
'£2 U

0 0'f y> 3	jz, !'
' l
. laji |	-v1	r 3—tq
¦®	{ —i I J i
	i	.Si	I	I."
JZ/'J l-J
i	1^1	j ,1	||'
i	~ti	. 3 ll
r	I
I
J
i_j ^ I -1 •—(^-1 ~'j:.! £3 0 '1 0 0 * ' r2?':J I '—(! 5 l-J *1 E3 0 lEf y
I
0 4
TIME, days
Figure C.252. Total solids concentration of influent synthetic
sewage to the first unit in the three unit
Cornell NTT system in Phase 1. First day of
analysis was February 16, 1981.
1 0M0 		
I
3~ri i.
... - |
30 0 |
i
70 0 -
E0 0 -
E0 0 • ¦
1-10 0 -i-
'•h-i
360 -pT,
SQ 01 -¦
¦i y 8 -¦
O --

J34?
I

a—

i
!3
3—53
I |

1A
a
¦i
¦_i EU0 iti Eu 3u '1 tif 1 20 1 10 1 Ed 1 By 2t)y
TIME, days
Figure C.253. Total solids concentration of effluent synthetic
sewage from the first unit in the three unit
Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
565

-------
00
S
to
H
1 50 0
•1 35 0 --
•1EQ0--
1 05 0 - ¦
50 0
750 4
50 0
L-iS'-J
30 0
•15 0 --
0
Q
10 20 30 1-0
0 50 70 By 3 0 -1 tn-1
Figure C.254.
TIME, days
Total solids concentration of influent synthetic
sewage to the second unit of the three unit
Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
60
e
w
Eh
•'1 00 0
I
~ 0 0 4-
50 0
7 0 0 - -
50 0 ...
50 0 --
1-10 0 ..
30 0 J?
p5?.
raj L	
J© 0 + yd ^
iH
• 1 0 0 +
0

^ L-lV'
-a i i
Figure C.255,
>-i0 50 By iW0 150 1 10 -1 Em -1B0 E'.-ii-J
TIME, days
Total solids concentration of effluent synthetic
sewage from the second unit of the three unit
Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
566

-------
1 80 0
BQ 0
B00
70 0
B0 0
50 0
00
6 10 0
H 300
20 0
1 0 0
0
0 20 L-i0 B0 B0 '10 0 -150 '1 L-i© -1 E0 -1 B0 200
TIME, days
Figure C.256. Total solids concentration of effluent synthetic
sewage from the third unit in the three unit
Cornell NFT system in Phase 1. First day of
analysis was February 1, 1981.
1 00 0
30 0
~ 0 0
700
50 0
50 0
"m 1-10 0
e
30 0
t/3
> —, _ _
h sy u
1 0 0
0
Q 20 *-10 E0 BQ -10 0 "120 1 1-.0 1E0 1B0 200
TIME, days
Figure C.257. Total volatile solids concentration of influent
synthetic sewage to the first unit in the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 16, 1981.
F
~	I

a


567

-------
-p-
5G 0 !
i
EQ0 -f-
70 0 --
50 0 --
50 0 --
L,00 ..
30 0 -¦
50 0 --
i -J 0 -L '
ifl.

P-	1, *
r	a

0 Ea i-i0 5u aa 100 -120 -1 m? 1t.0 IB0 E00
TIME, days
Figure C.258. Total volatile solids concentration of effluent
synthetic sewage from the first unit of the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.
i-, M 0
3B0-.
35 0 -¦
500-
B1-! 0
E0 0 - -
-1 B 0
•1S0
B0
Li-S^
-- V
H
a- <1 \
6
L-10 - ¦
0
0

•10 50 30 >-10 5 0 E0 70 B 0 3 0 100
Figure C.259,
TIME, days
Total volatile solids concentration of influent
synthetic sewage to the second unit of the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 16, 1981.
568

-------
60
s
to
>
H
•1 0H 0 —
i
30 0 ~.
£30 0 -
73 0 --
i
50 0 --
5 01 0 f
L-. 0 0 .1
!
30 0 --
20 0

0 0 t / "-P-
- i *
0 -I	—H	
¦a1?
'l
-El.

tar
'its
:~n
—t-
0 EG L-I0 E0 du -10 0 1 Eu -I •—<0 -1E0 --1B0 500
TIME, days
Figure C.260. Total volatile solids concentration of effluent
synthetic sewage from the second unit of the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.
1 ! I
CO
6
GO
>
H
TIME, days
Reproduced from 0^%
best available copy.
Figure C.261.
Total volatile solids concentration of effluent
synthetic sewage from the third unit in the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.
569

-------
-1 00 0
30 0 --
BQ O --
70 0 --
5 0 0 - -
50Q --
l—10 0 ..
30 0 --
Ji
aoy ad I
-1 Q 0 4-
0 -I	1	1-
0 EQ "-iQ

—^ JA

i ^
tab
Eu a0 -10 0 -I 20 -1 t-,0 1E0 -1B0 500
TIME, days
Figure C.262. Total fixed solids concentration of influent
synthetic sewage to the first unit in the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 1, 1981.
bO
6
w
P*
H
• 1 00 0
50 0 -¦
E30 0
70 0
50 0 --
50 0 ¦ -
1-10 8 ...
~3 0i 0 - -
•1 0 8 \
5?
i\
cs—
-a5? p
I • '
d '•? p"
' >n
. j
-1

0 20 '-10 E0 3 0 "1'6 0 120 -1 1-.0 •'! !31.1 lb'd ayw
TIME, days
Figure C.263. Total fixed solids concentration of effluent
synthetic sewage from the first unit in the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.
570

-------
50 0 J-
L-.B0 --
'—100 -¦
350-.
300-
E50--
500 El
-1 5 0 - -
1 0 0 - -
50 -¦
0--
0
'o-a. Bi
a
A


-t-
•10 E0 30 h0 50 50 70 50 30 '100
Figure C.264.
TIME, days
Total fixed solids concentration of influent
synthetic sewage to the second unit in the three
unit Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
M
T~
I
d:d0 |
I
B0 Fi |
706 1
U 4
a
n
M
TIME, days
Figure C.265. Total fixed solids concentration of effluent
synthetic sewage to the second unit in the three
unit Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
571

-------
C/3 .. .
H	. .... ¦
;. . •:. i. "'
:='!j	E3 :li •':/ : V ¦	0	rr' jt-il
TIME, days
Figure C.266. Total fixed solids concentration of effluent
synthetic sewage from the third unit of the three
unit Cornell NTT system in Phase 1. First day of
analysis was February 16, 1981.
TIME, days
Figure C.267. Total suspended solids concentration of influent
synthetic sewage to the first unit in the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 16, 1981.
572

-------
TIME, days
Figure C.268. Total suspended solids concentration of effluent
synthetic sewage from the first unit in the three
unit Cornell NTT system in Phase 1. First day of
analysis was February 16, 1981.
L-,0 T
3E -¦
BE-
SS-.
E0 --
Q -10 20 30 L-<0 B0 E0 70 BfcJ ¦ 3h -1
Figure C.269. Total suspended solids concentration of influent
synthetic sewage to the second unit in the three
unit Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
TIME, days
573

-------
M
e
co
w
H
' 1 0 0
B 0
E0
7 0 .i.
5 0
5 0 -
i—.0 --
3 0 --
50-13
L 3 !3
0
~
eg
-A' \r
ti ifi
—i	y	
1
0 5h-J Liti SO Bu '1 0 0 120 -I L-itJ -1E0 -1B0 200
TIME, days
Figure C.270.
Total suspended solids concentration of effluent
synthetic sewage from the second unit in the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.
-1 9 0
3 0
B 0
7 0
5 0
^ 5 ©
60
B L_, y
H
5 0
1 0
0
0 50 L_I0 EO B 0 -10 0 -150 1 L-.0 1 E 0 -1BQ 500
TIME, days
Figure C.271. Total suspended solids concentration of effluent
synthetic sewage from the third unit in the three
unit Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.

-fei-



ESGj
-sj
!
.a
I. J
i
5TU

-------
•1 0 0
0 ae '-10 E0 B0 100 -150 1 L-10 -1E0 130 E00
TIME, days
Figure C.272. Volatile suspended solids concentration of
influent synthetic sewage to the first unit in
the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
•1 0 O
30
B0
"7 0
Ea 0
^ 5 0
to
6 L-,0
£ 3 0
>
50
1 0
0
f	i—if Ey B f 1 y0 1 Efc) '1 L-i0 1 E0 1 B0 B00
TIME, days
Figure C.273. Volatile suspended solids concentration of
effluent synthetic sewage from the first unit in
the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
575

-------
I—.0
30 -1 00
TIME, days
Figure C.274.
Volatile suspended solids concentration of
influent synthetic sewage to the second unit in
the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
1 Q0
0 2fcJ	EkJ B 0 -10 0 -120 -1 t-.0 -1E0 -1B0 200
TIME, days
Figure C.275. Volatile suspended solids concentration of
effluent synthetic sewage from the second unit
in the three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
576

-------
•1 0 0
~	0 --
~	0 --
7 0 ..
E0 --
1 50-
« i—i y ..
wa
CO
> 30-
20
-1 0
0
iafW-T

H	&—h
\ N
¦®ei	1—
0 E0 L-.0
Figure C.276.
E0 B0 -10 0 -120 -1 Li0 150 1 B0 200
TIME, days
Volatile suspended solids concentration of
effluent synthetic sewage from the third unit in
the three unit Cornell NTT system in Phase 1.
First day of analysis was February 16, 1981.
oo
E
w
ui
h
Ba-
0 50
Figure C.277.
* 0 5 ti B y 11 hj '-zJ i EE ti *1 '-i f -*1 E tj 'IS y E 0 ti
TIME, days
Fixed suspended solids concentration of influent
synthetic sewage to the first unit in the three
unit Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
577

-------
1 Q 'r"_l
BO --
~ Q --
7 0..
E 0 --
5 0 --
Li0 .
30 -
50 -¦
1 10
- ,J3 PI
y ilnl iisuii o-
133s?
f	—ggfci—-""P

a eu "10
Gfc.1 B 0 '1 00 -1 20 '1 L-itJ '1 1=3 0 '1 E 0 2tJ0
TIME, days
Figure C.278. Fixed suspended solids concentration of effluent
synthetic sewage from the first unit in the three
unit Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
-1 0 -p
~
B --
E
0 10 50 30 ^-10 5 0 E0 70 B0 3d -100
TIME, days
Figure C.279. Fixed suspended solids concentration of influent
synthetic sewage to the second unit in the three
unit Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
578

-------
CO
co
pf
• i 0 0 -p
30..
~ © -
7 3 • -
513 --
_ 0 '•
i_,e
3G-
50 --
•I 0 ..
= lTi
00
B

f itoOuBEma-
.3^ ,
—•-fl gj ij |—fesgja-

0
20 L-i0
E0 E0 1 My ''1 :zZ'1 I—irli '1 ~ tl I Id 0 Et'tl
TIME, days
Figure C.280.
Fixed suspended solids concentration of effluent
synthetic sewage from the second unit in the
three unit Cornell NFT system in Phase 1. First
day of analysis was February 16, 1981.
•1 £

3 0
* f
oo
g
in
co
TIME, days
Figure C.281. Fixed suspended solids concentration of effluent
synthetic sewage from the third unit in the three
unit Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
579

-------
0 a 5 0
Id ~Ll5
0 o l1 0
0.35
klJ ~ 3 0
0.ES
o*
"m 0 o 2 0
6
«0o 1 5
c
U fcj a 1 0
0.05
0
0 -15 30 >-15 E0 75 30 -1 05 -120 -135 -150
TIME, days
Figure C.282. Cadmium concentration of influent synthetic
sewage to the first unit in the three unit
Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.

ik'
f5*
I %
p
I	I
\
\ ,sl I
iS rod
Al
\	k
<* 'J
I O
n
o
i
"1 0 +
IEl_
_.='£r\ '-I	;-]	-
	'a	 !1. _ ; .1.
•'/—T-	,	.	I	,
0 i £ ~'.j '-i5 5 0 ~5 E0 -i Z-E 150	-150
TIME, days
Figure C.283. Cadmium concentration of effluent synthetic
sewage from the first unit in the three unit
Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
580

-------
0.S5
0 .S3-
0 a a 0 ¦ -
0.17-
0 .'1 5 ¦¦
0.i a--
0 . '*1 0 -¦
0 .07 --
0.05--
0.0Sjf'
0 --
0

\ / \ .a.
-to—ftr i o~ i

30	•-( 0 50 E0
TIME, days
-t-
70 B0 30 -100
Figure C.284. Cadmium concentration of influent synthetic
sewage to the second unit in the three unit
Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
a oi-j
H
J .3 ^ J i-
I
0 n 3 E: I
i
• J o '.J j"
'—
f 0=as|
O* 0 n 2 0 j-
O
0 j'ISt
0 a -1 © L

.a
TIME, days
Figure C.285. Cadmium concentration of effluent synthetic
sewage from the second unit in the three unit
Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
581

-------
0 o 5 0
0
0 ~ 1-10 -¦
0=35 --
0=30 --
0,E5-
60 m ~ EE y ••
s
Q
o
0 a 1 5 --
0 a-1 0 --
0a0SnErc?
!*>
qi to
J


0
0
b^ap-T"
-15 30
	1	h-
"-,5 S0
—I	t—
¦y
7 5 30 -1 05 1 5 0 -1 35 1 50
TIME, days
Figure C.286. Cadmium concentration of effluent synthetic
sewage from the third unit in the three unit
Cornell NFT system in Phase 1. First day of
analysis was February 16, 1981.
00 o
qsi
AfVtrf
0 15 30 i-,5 E 0 7 5 50 "1 05 "1 3 0 -13 5 "1 50
TIME, days
Figure C.287.
Soluble cadmium concentration of influent
synthetic sewage to the first unit in the three
unit Cornell NFT system in Phase 1. First day
of analysis was February 16, 1981.
582

-------
00
6
Q
U
iJ
o
w
f a ' 1

A
~
-a-a
'^GeT3
-1 S 30
h3 0 7 5 Shi
TIME, days
•1 Q5 -1 2 0 -1 3 5
50
Figure C.288. Soluble cadmium concentration of effluent
synthetic sewage from the second unit in the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
60
s
Q
o
hJ
O
w
0 D 5 5 -|	
0.53-
0.S0"
0 ¦ "1 ? - .
a 0-i 5--
0 D-1 a ¦¦
0.10-
0O07--
0 o 0 5 - ¦
0.0a!^
0	
0
—ioooDiy i
•10 30 30
i-i 0 50 E0 70 a 0 3 0 -100
TIME, days
Figure C.289.
Soluble cadmium concentration of influent
synthetic sewage to the second unit in the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
583

-------
TIME, days
Figure C.290. Soluble cadmium concentration of effluent
synthetic sewage from the second unit in the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
0 . ^ :=j -	j
0^0}	i
s.asj	;
0.30|	I
0 3 E S 4	j
0 a 2 0 i
_ I
U a 1 ••-
0 n 1 0 -
0 a 3 5 • -	a
0 mdq -n——		i
0 *1S 30 L-i5 E 3 -E 30 1 0S 1E0 13 5 -1 3Q
TIME, days
Figure C.291. Soluble cadmium concentration of effluent
synthetic sewage from the third unit in the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 16, 1981.
58U

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I 0 ..
TIME, days
Figure C.292. pH of influent synthetic sewage to the first
unit in the three unit Cornell NFT system in
Phase 1. First day of analysis was February
16, 1981.
	1	1	i	y	» , , —I	i	1	i	i
3'd* '¦-i I—' I lii*-*_! 1 '—i! bz -iJ *'! 5 i^.1 EE'cmJ
TIME, days
Figure C.293. pH of effluent synthetic sewage from the first
unit in the three unit Cornell NFT system in
Phase 1. First day of analysis was February
16, 1981.
585

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TIME, days
Figure C.294. pH of influent synthetic sewage to the
second unit in the three unit Cornell NFT
system in Phase 1. First day of analysis
was February 16, 1981.
-1 Q
_._a- :%ELaCr E

0 ay L-iQ EG BQ 10a 1 50 -1 "-iQ -1E0 -1B0 200
TIME, days
Figure C.295. pH of effluent synthetic sewage from the
second unit in the three unit Cornell NFT
system in Phase 1. First day of analysis
was February 16, 1981.
586

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W 20 *-10
EQ B O -1 0fcJ 1 20 1 'iO '1 E0 -1 BtJ 200
TIME, days
Figure C.296.
pH of effluent synthetic sewage from the third
unit in the three unit Cornell NFT system in
Phase 1. First day of analysis was February
16, 1981.
x
a
TIME, days
Figure C.297. pH of filtered influent synthetic sewage to the
first unit in the three unit Cornell NFT system
in Phase 1. First day of analysis was February
16, 1981.
58?

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I 0
•1 0
3
3
B
B
X ?
a.
T7
bi
S
5
0 EG L-.0 50 B0 10 0 1 S0 1 i-i0 -1E3 -1 ~ 0 200
TIME, days
Figure C.298. pH of filtered effluent synthetic sewage from
the first unit in the three unit Cornell NFT
system in Phase 1. First day of analysis was
February 16, 1981.
tPI
A
i \
a
rj'^ >T
y i 11
i iaa
ft !
—a i
iisE#. }
"4
rj
j

~
a ca
i
¦x
a.
i _
'I 'V
•'! 1

-3 3-3
3 3
TIME, days
Figure C.299.
pH of filtered influent synthetic sewage to
the second unit in the three unit Cornell NFT
system in Phase 1. First day of analysis was
February 16, 1981.
588

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1 0 j
¦10..
~ A \
1
I la? psj ,i
k
--Vj
1 b CEW-si

tei
n
4 nf£r-~
V
f 2Q L-I0 E0 B 0 *1 tl 0 1 E0 "1 L-t0 '1 E0 '1 ~ 0 E00
TIME, days
pH of filtered effluent synthetic sewage from
the second unit in the three unit Cornell NFT
system in Phase 1. First day of analysis was
February 16, 1981.
Figure C.300.
t.1 2W '—i 0
50 B0 1 k) hi '1 E0 1 L-itl '1 Ed 1 B'J 2tltl
TIME, days
Figure C.301.
pH of filtered effluent synthetic sewage from
the third unit in the three unit Cornell NFT
system in Phase 1. First day of analysis was
February 16, 1981.
589

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-IMZ, days
igure C.302. Dissolved oxygen concentration of
influent sewage delivered to the first
unit of the three unit Cornell NFT
system in Phase 1. First day of
analysis was February 19, 1961.
!
1
i
f
' 3 o 3 12 13 " 3 r3 1 '-i rzi "P~
TIMS, days
Figure C.303. Dissolved oxygen concentration of influent
synthetic sewage at Point A of the first
unit of the three unit Cornell NFT system
in Phase 1. First day of analysis was
February 19, 1981•
590

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w
s
o
Q
bD
E
o
Q
S3 E
Figure C.30U.
•1 E -15 -1B E1
TIME, days
3Q
Dissolved oxygen concentration of influent
synthetic sewage at Point B of the first
unit of the three unit Cornell NFT system
in Phase 1. First day of analysis was
February 19, 1961.
0
Figure C.305.
3 -13 -15 1B 31 Sh 3? 3Q
TIME, days
Dissolved oxygen concentration of effluent
synthetic sewage from the first unit of
the three unit Cornell NFT system in
Phase 1. First day of analysis was
February 19, 1981.
591

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to
E
O
Q
8
-1 a
ai-. a? 30
¦15 -1 a a-i
TIME, days
Figure C.306. Dissolved oxygen concentration of influent
synthetic sewage to the second unit of the
three unit Cornell NFT system in Phase 1.
First day of analysis was February 19, 1961
to
G
o
Q

15 1 a a^
TIKE, days
ai-. a? 30
Figure C.307-
Dissolved oxygen concentration of influent
synthetic sewage at Point A of the second
unit of the three unit Cornell NFT system
in Phase 1. First day of analysis was
February 19, 1981.
592

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-1 0
a
B
T7
* B
60
e 5
° u
Q H
3
S
-1
0
0 3 B a -15 -15 IB a-1 Sh a? 30
TIME, days
Figure C.308. Dissolved oxygen concentration of influent
synthetic sewage at Point B of the second
unit of the three unit Cornell NFT system
in Phase 1. First day of analysis was
February 19» 1981.
-1 0
3
B
7
c* B
s ^
o" ^
Q
3
a
•1
0
03 B a -IE -15 -1B a-1 Eh S7 3©
TIMS, days
Figure C.309* Dissolved oxygen concentration of effluent
synthetic sewage from the second unit of
the three unit Cornell NFT system in Phase 1.
First day of analysis was February 19, 1981.
593

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¦£»
0 3
Figure C.310.
3 -1 S -15 -IB E'l S1-! S7 30
TIME, days
Dissolved oxygen concentration of influent
synthetic sewage to the third uhit of the
the three unit Cornell NFT system in Phase 1.
First day of analysis was February 19, 1980.
-1 0
0 3
Figure C.311
•1 a 15 -1 b
TIME, days
El ah
30
Dissolved oxygen concentration of influent
synthetic sewage at Point A of the third
unit of the three unit Cornell NFT system
in Phase 1. First day of analysis was
February 19, 1980.
59^

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to
s
o
Q
Q
Figure C.312.
1 a is -ib
TIME, days
-1 s>-. a? 30
Dissolved, oxygen concentration of influent
synthetic sewage at Point B of the third
unit of the three unit Cornell NFT system
in Phase 1. First day of analysis was
February 19, 1901.
0
•1 a i 5 -1 a a 1
x —' i£j 9 day s
ai-i
7" 30
Figure C.313.
Dissolved oxygen concentration of effluent
synthetic sewage from the third unit of
thecthree unit Cornell NFT system in
Phase 1. First day of analysis was
February 19, 1931•
595

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-1 1 0
Tj
I
cS
<=*
(n
O
Sh
W
CIME, days
Figure C.31^. Evapotranspiration from the three unit
Cornell NFT system in Phase 1. First
day of measurement was February 17, 1981.
596

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so
0 EG 1-1 1 Ey S3 -10 9 120 I L-i0 Sy l eifc?©
TIME, days
Figure C.315. Incident solar radiation at Ithaca, NY,
over the Cornell NFT experimental period.
First day of measurement was October 1,
1979-
ir\
o
H
X
>-3
h -f
E-"
H
§
cc

:2
-7T '7i
73 '-v
-i..-

	 ^ i7j ...	".w
"" J ¦	inr1	'
:	.	.'I	^ W. : \	1 «

i.:.j . i-J •- -1
':-2 '
- v			——	-	
200 220 2U0 260 280 " 300 320 3^0 360 380 U00
TIME, days
Figure C.315- (continued) Starting date of this figure
is April IT, 1980.
597

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3 0 T
a
f f
G
' UOO b2Q U60 kQO~ 500 520 5^0 ?60 580 600
TIME, days
Figure C.315. (continued) Starting date of this figure
is December 3, 1980.
598

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