Analysis of carbonaceous aerosols using the Thermal
Optical Transmittance and Thermal Optical
Reflectance Methods
99-439
Gary A. Norris*, M. Eileen Birch**, Michael P. Tolocka*, Charles W. Lewis*, Paul
A. Solomon*, James B. Homolya***
* U.S. EPA, ORD/NERL, Research Triangle Park, NC 2771 1
** National Institute for Occupational Safety And Health, Cincinnati, Ohio 45226-1998
*** U.S. EPA OAQPS, Research Triangle Park, NC 27711
ABSTRACT
Carbonaceous particulate Typically represents a large fraction ofPM;>.5(20 - 40%). Two
primary techniques presently used for the analysis of particulate carbon arc Thermal
Optical Transmission (TOT - NIOSII Method 5040) and Thermal Optical Reflectance
(TOR). These two methods both quantify carbon by heating filters and volatilizing the
carbon that is oxidized in a granular bed of Mn02, reduced to CH4 in a Ni methanator,
and quantified as CH.i with a flame ionization detector. However, the methods use
different techniques to correct for the formation of pyrolysis products and the temperature
programs for defining organic and elemental carbon. The TOT and TOR measurement
techniques are being compared using samples from the Chemical Speciation Monitor
Evaluation Field Study. All of the samples will be measured with TOR and a subset of
samples representing a range of mass concentrations will be measured with TOT. This
comparison will provide insight into the effect of the measurement technique parameters
on organic and elemental carbon concentrations.

-------
INTRODUCTION
Carbonaceous particulate represents a significant fraction of airborne particulate matter
less than or equal to 2.5 |im in aerodynamic diameter (PM2.5). Carbonaceous particulate
is classified into three main categories: organic carbon (OC), elemental carbon (EC), and
carbonate carbon (CC)1. These categories are operationally defined by the method or
analysis technique. OC sources include combustion, industrial, and photochemical
process. EC sources are mainly combustion related. CC is found in soil and generally
constitutes less than 5 percent of the total carbon2. OC and EC typically represent 20 to
40 percent of PM2.5. As a result, quantifying OC and EC is required for reconstruction of
the gravimetric mass. In addition, OC and EC can be used in receptor models and as
exposure variables for health effects studies.
Two primary thermal-optical methods are used to quantify particulate carbon: thermal
optical reflectance (TOR) and thermal optical transmission ( TOT). These two methods
both quantify carbon by heating a quartz filter punch (0.5 cm2 for TOR and 1.5 cm2 for
TO T) and volatilizing the carbon which is oxidized in a granular bed of MnN02, reduced
to CI-I4 in a Ni methanator, and quantified as methane (CH4) with a flame ionization
detector. However, the methods differ in the technique used to correct for the formation
of pyrolysis products and they use different temperature programs.
The TOR particulate carbon analysis method has been previously described by Chow2.
The temperature profile and the range of times required for the response at each step to

-------
become constant is shown in Table 1. The rumple oven is stepped to 500 °C in 4 steps
(120 °C (OC1), 250 °C (OC2), 450 °C (OC3), 500 °C (OC4)) to vaporize the organic
carbon in a helium atmosphere. In the second part of the analysis a 2 percent (V98
percent He mix is introduced, the temperature is then stepped to 800 °C in 3 steps (550
°C (EC1), 700 °C (EC2), and 800 °C (EC3)). The amount of time for each temperature
step is operationally defined based on the time required for the FID response to return to
baseline. The result of this temperature step routine is the OC and EC are quantified in
terms of the temperature steps (OC1, OC2, OC3, OC4, EC1. EC2, EC3). Methane (CH4)
calibration gas is introduced at the end of each sample cycle. Pyrolysis correction is
made by monitoring the filter reflectance of a lle-Ne laser at 632.8 nm with a
photodetector. Pryolizcd OC is quantified as the carbon evolved from the time the carrier
gas is changed from He to 2 percent O2 in He to the time the laser measured filter
reflectance reaches its initial value. Carbonate carbon Ls determined by acidifying the
sample with HC1 and measuring evolved carbon at ambient temperature in a 2 percent
oxygen/98 percent helium atmosphere.
The TOT method used in this analysis in specified in NIOSH Method 5040 3*4. In this
method, the sample oven is purged with helium and the temperature is stepped (to 205
°C. 500 °C, 650 °C, and 850 °C) to volatilize the OC. and CC. In the second part of the
analysis the temperature is lowered to 650 °C, and a 2 percent O2/98 percent He mix is
introduced, the temperature is then stepped to 940 °C. Table 1 specifies the temperature
program and time periods for the steps. At the end of the analysis, a calibration gas
standard (CH4) is introduced. Correction for pyroloysis of the OC is accomplished by

-------
monitoring the 'ransmittance of a He-Ne laser though the filter at 670 nm The point at
which the filter transmittance reaches its initial value is defined as the split between OC
and EC. Carbonate carbon is determined using HC1 pretreatment or the thermogram will
show a quant ifiable peak around 820 °C. The National Air Monitoring Station (NAMS)
chemical spcciation program specifics the NIOSH Method 5040 as the method for OC,
EC, and total carbon (TC = EC + OC).
The TOR and TOT methods were evaluated in a methods comparison in 1986 5.
Comparisons were based on ambient PMi0, automotive, wood smoke, pvrolized ambient
PMio, organic aerosol from a smog chamber, and a blank. Results were presented for the
laboratories used for this study with Desert Research Institute (DR1) and Sunset Labs
(SL) conducting the TOR and TOT analysis, respectively. The coefficient of variation
for the TC. OC, and EC for the ambient samples for TOR and TOT were 3.0, 2.5, 3.0
percent; and 2.2, 2.5, and 6.2 percent, respectively. Results for each laboratory were
reported as TC normalized to the mean,of all of the participants (n - 10) and the ratio of
EC to TC (see Table 2). The TC results for both of the methods were similar, however,
the EC/TC ratio for TOT was lower than TOR for the ambient, unleaded auto, and wood
smoke samples. Wood smoke had the largest difference with TOR and TOT having
EC/TC ratios of 0.36 and 0.09, respectively. An interlaboratory comparison between
TOR (DR1) and TO T (SL) was conducted as part of the DRI study on Phoenix and
Tuscon Urban Haze and PMjo. This study also found good agreement on three TC
measurements of potassium hydrogen phthalate, with an average absolute percent
difference less than 3 percent2.

-------
The TOR and TOT methods were also evaluated in an interlaboratory comparison6.
Samples from an urban location, a loading dock with diesel vehicles, and a firehouse bay
were evaluated. The comparison also included filters spiked with sucrose, and disodium
salt of ethylenediaminetetraacetic acid (EDTA) that have no EC to evaluate the TC and
the pryrolysis correction. Table 3 summarizes the results from the methods comparison.
TOR results are from one laboratory while the TOT results represent an average of 5
laboratories. Both the TOR and TOT techniques quantified less than 3 percent of the
EDTA and sucrose standards as EC. The absolute percent difference between the TOR
and TOT methods for the EC measurements were 33, 76. 58, 27, 23 for the wood 1 , wood
2, urban, dicsei truck, and fire station samples, respectively. The absolute percent
difference between the TOR and TOT for the OC measurements were 1, 6, 3 for the
diesel truck, and lire station samples, respectively. EC had largest percentage difference
for the wood smoke and urban samples. This difference in the EC measurements may be
due to either the temperature program, length of analysis at each temperature, ( Table 1)
or the method used to correct for pryrolysis of the OC.
EXPERIMENTAL DESIGN
This comparison of the TOR and TOT particulate carbon analysis techniques will use
ambient PM2.5 samples from 3 cities with different sources of carbon; and quality
assurance standards of sucrose, wood smoke, diesel, dicsei spiked with carbonate, and

-------
blank filters (Table 4). Sunset Laboratory will conduct the TOT analysis using NIOSH
Method 5040 and DRI will conduct the TOR analysis following the temperature program
in Table 1.
Quality assurance samples were sent to each laboratory. Sunset Laboratory and DRI will
each be sent a sample set: sucrose solution and a 47-mm quartz filter, carbonate solution,
quartz filter with diesel particulate, and a quartz filter with wood smoke particulate. To
determine the potential interference of carbonate when present in a carbonaceous
particulate sample (in this case diesel), the sample portion would be analyzed with and
without a spike. The labs should obtain the same EC results in both cases if
carbonate does not interfere. All of the standards will be provided in triplicate to allow
for calculation of the measurement precision of the calibration and source samples.
The ambient samples will be from the Philadelphia and Rubidoux PM2 5 Chemical
Speciation Sampler Evaluation sites, and the Spokane Particulate Matter and Health
Study 1. Philadelphia particulate carbon is a mix of automobile and industrial source
while Rubidoux is a mix of automobile and photochemical organic aerosols. The
Philadelphia and Rubidoux samples will be evaluated with and without XAD denuders
upstream of the quartz filter to remove organic gases. Spokane filters will be collected in
November and December when the particulate carbon is predominately from wood
smoke7. Two additional wood smoke source samples from EPA will also be evaluated.
Samples from the 3 cities and the source samples will represent a range of OC
concentrations and FC/OC ratios. Differences between the methods will be highlighted

-------
by using the combination of samples from cities with different sources of carbon, and
source samples (see Table 4).
CONCLUSIONS
Particulate carbon typically represents a large fraction of PM2.5. OC and EC
measurements arc routinely used for reconstruction of the gravimetric mass, and receptor
modeling. The two primary carbon analysis methods arc TOR and TOT. OC and EC
arc determined operationally by each of the methods. These techniques differ in their
temperature programs, step time, and pryrolysis correction. Differences between the
methods need to be fully understood to help interpret past and future particulate carbon
results.
This comparison of the TOR and TOT particulate carbon analysis methods will add to the
analyses conducted by Countess5 and Birch6. Analysis of PM2 5 samples from three cities
with different sources of particulate carbon will help provide information on any
systematic differences in the OC and EC measurements. In addition, source samples of
diesel and wood smoke will be analyzed to evaluate the measurement precision.
Particulate carbon measurements of samples with and without an XAD denuder will
provide data on the differences between the TOR and TOT methods for samples which
do not have the potential OC artifact caused by gas phase organic compounds. Organic
gas denuders have not been used in previous intcrcomparisons.

-------
DISCLAIMER
This paper has been reviewed in accordance with the U.S. Environmental Protection
Agency's peer and administrative review policies and approved for presentation and
publication. Mention of trade names or commercial products does not constitute
endorsement or recommendation for use.

-------
REFERENCES
1.	Fung, K. Aerosol Sci. TechnoL, 1990, 12, 122-127
2.	Chow, J.C.; Watson, J.G.; Pritchett, L.C.; Pierson, W.R.; Frazier, C.A.; Purcell,
R.C. Atmos. Environ, 1993, 8, 1185 - 1201.
3.	Birch, MA. Analyst 1998,123, 851-857
4.	Eller, P.M.; Cassinelli, M.E. Elemental Carbon (Diesel Particulate): Method 5040.
N10SH Manual of Analytical Methods, 4th ed. (1st Supplement) National Insitute for
Occupational Safety and Health, DliliS (N10S1I), Cincinnati, Oil; Publicaiton No. 96-
135, 1996.
5.	Countess, R.J. Aerosol Sci. TechnoL, 1990, 12, 114-121
6.	Birch, M.H.; Cary, R.A. Aerosol Sci. TechnoL, 1996. 25, 221-241
7.	Haller, L.; Claiborn C.; Larson, T.; Kocnig J.; Norris G.; Edgar R. J. Air Waste
Manage. Assoc.

-------
Table 1. Optical correction and thermal programs for the TOT and TOR particulate
carbon measurement techniques.		
Method



TOT



Optical correction
Transmission


Program
OC

EC
Helium
250 °C, 1 min
2 % O2 in Helium
650 °C, 30 sec

500 °C, 1 min

750 °C, 30 sec

650 °C, 1 min

850 °C, 1 min

850 °C, 1.5 min

940 °C, 2 min

Reduce to 650 °C






TOR



Optical correction
Reflectance


Program
OC

EC
Helium
OC1: 120 °C,
3 - 10 min
2 % O2 in Helium
EC1: 550 °C
3-10 min

OC2: 250 °C,
3-10 min
OC3: 450 °C,
3 - 10 min
			
FC2: 700 °(\
3-10 min
EC3: 800 °C,
3-10 min

OC4: 550 °C,
3 10 min






OC - OC1 t OC2 t OC3 + OC4 pryrolized carbon
EC - EC1 + EC2 -* EC3 pryrolized carbon
Table 2. Comparison of the TOR and TOT analysis methods (Countess)
TC normalized to the mean of the 10 participants in the comparison
Method
Ambient PMm
Unleaded Auto
Diesel
Wood smoke
sample 1
TOR
1.00
0.98
1.00
1.02
TOT
0.99
0.88
0.95
0.^4
Mean loading
((ig/cm2)
30.8
34.0
106.8
96.7

IC/TC ratio
Method
Ambient PM10
Unleaded Auto
Diesel
Wood smoke
sample 1
TOR
0.30
0.83
0.87
0.36
TOT
0.15
0.70
0.88
0.09
Mean ratio
0.22
0.72
0.81
0.16

-------
Tabic 3. Comparison of the TOR and TOT analysis methods (ng/cm2) (Birch)

TOR
TOT
Sample
OC
EC
OC
EC
Sucrose
0.57
0.26
EDTA
0.20
0.02
Wood 1
6.20
4.42
Wood 2
—
2.17
—
0.30
Urban
9.70
3.00
10.42
1.65
Diesel Truck
17.33
8.20
18.48
6.25
Fire Station
136
20.27
139.80
16.10

-------
Table 4. Samples to be analyzed using TOT and TOR
Speciation Site
Number of
Samples

W/O XAD Denuder


Philadelphia
10
Carbon from motor vehicles and
industrial processes
Rubidoux
10
Carbon from motor vehicles and
secondary aerosols
Spokane
4
Carbon from motor vehicles and
wood smoke
Field blanks
4
2 field blanks from Philadelphia,
and Rubidoux



Wood smoke source
samples
2
2 wood smoke source samples from
the EPA, Research Triangle Park.
NC
Source sample blank
1
1 source sample blank from the
EPA, Research Triangle Park, NC


With XAD Dcnudcr


Philadelphia
5

Rubidoux
5




Quality Assurance


Sucrose
3
Triplicate evaluation of the
calibration
Wood Smoke
3
Triplicate evaluation of a wood
smoke sample
Diesel
3
Triplicate evaluation of a diesel
sample
: Diesel spike with carbonate
3

Field blanks
2


-------
NERL-RTF-O-647 TECHNICAL REPORT DATA
I. REPORT NO.
EPA 600/R-99/059
2.
3.RECIPIENT'S ACCESSION NO.
4. TITLL AND SUBTITLE
Analysis of carbonaceous aerosols using the Thermal Optical Transmittance and
Thermal Optical Reflectance Methods
5.REPORT DATE
6.PERFORMING ORGANIZATION CODE
7. AUTHOR(S)
Gary A. Norris*, M. Eileen Birch**, Michael P. Tolocka*. Charles W. Lewis*. Paul A.
Solomon*, James B. Homolya***
•	U.S. EPA, ORD/NERL, Research Triangle Park, NC 27711
" • National Institute for Occupational Safety And Health, Cincinnati, Ohio 45226-
1998
*	* • U.S. EPA OAQPS, Research Triangle Park, NC 27711
8.PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
USFPA/NFRL/SACB
79 TW Alexander Drive
MD-47
RTP, NC, 27711
10.PROGRAM ELEMENT NO.
11. CONTRACT/GRANT NO.
RTI Contract 68D50040, WA 111 30
12. SPONSORING AGENCY NAME AND ADDRESS
USHPA/NERL/SACB
79 TW Alexander Drive
MD-47
RTP, NC, 27711
13.TYPE OF REPORT AND PERIOD COVERED
14. SPONSORING AGENCY CODE
15. SUPPLEMENTARY NOTES
16. ABSTRACI
Carbonaceous paniculate typically represents a large fraction of PM?S (20 -40%). Two primary techniques presently used for
the analysis of particulate carbon are Thermal Optical Transmission (TOT - NIOSH Method 5040) and Thermal Optical
Reflectance (TOR). These two methods both quantify Carbon by heating filters and volatilizing the carbon that is oxidized in
a granular bed of MnO,, reduced to CH4 in a Ni methanator, and quantified as CH4 with a flame ionization detector. However,
the methods use different techniques to correct for the formation ofpyrolysis products and the temperature programs for defining
organic and elemental carbon. The TOT and TOR measurement techniques are being compared using samples from the Chemical
Speciation Monitor Evaluation Field Study. All of the samples will be measured with TOR and a subset of samples representing
a range of mass concentrations will he measured with TOT. This comparison will provide insight into the effect of the
measurement technique parameters on organic and elemental carbon concentrations.
17. KEY WORDS AND DOCUMENT ANALYSIS
a. DESCRIPTORS
h.IDENTIFIERS/ OPEN ENDED
TERMS
c.COSATI



18. DISTRIBUTION STATEMENT
RELEASE TO PUBLIC

19. SECURITY CLASS (This
Report)
UNCLASSIFIED
21.NO. OF PAGES
12

20. SECURITY CLASS (This Page)
UNCLASSIFIED
22. PRICE

-------