EPA/600/A-95/085
Heterogeneous Photoreaetion of Formaldehyde
with Hydroxyl Radicals
Daniel Driscoll
Heterogeneous Chemistry and Aerosol Research Branch
AREAL EPA RTP North Carolina 27711
ABSTRACT
Atmospheric heterogeneous photoreactions occur between formaldehyde and hydroxyl
radicals to produce formic acid. These photoreactions not only occur in clouds, but also in
other tropospheric hydrometeors such as precipitation and dew droplets. Experiments were
performed by irradiating Teflon tubes containing nanomole concentrations of formaldehyde and
hydrogen peroxide. Following irradiation at a wavelength of 313 nanometers, the concentrations
of formaldehyde and formate ion were analyzed using High Pressure liquid Chromatography
and Ion Chromatography. Data are presented to illustrate how nitrate and sulfate anions effect
the photo-oxidation of formaldehyde in the aqueous-phase, as well as the effects of these anions
on the formate ion which is a product of this photo-oxidation. Formic acid and its precursor
formaldehyde are soluble in the aqueous-phase of the atmosphere. However, inhibition of the
atmospheric photo-oxidation reactions of these compounds coupled with evaporation of the
clouds or dew droplets can result in recycling the compounds from the liquid-phase back to the
gas-phase of the atmosphere.
INTRODUCTION
The literature contains little information regarding the heterogeneous chemistiy of
atmospheric organic acids and their precursors. Grosjean1 reports that in the urban air of the
California South Coast Air Basin, concentrations of formaldehyde range between 4-21 ppb,
while formic acid concentrations varied from 1-13 ppb. Also Keene and Galloway2 determined
that formic acid is responsible for 60% of the total acidity found in global non-urban
precipitation.
Emissions from anthropogenic activities as well as from natural sources combine to
contribute formaldehyde to the gas-phase of the atmosphere. Formaldehyde is readily soluble in
rain and cloud water. Figure 1 depicts the liquid-phase reactions of formaldehyde. After
formaldehyde enters the aqueous-phase, hydrolysis converts the carbonyl compound to
methanediol. This hydrate is stable in water solution. However, upon evaporation of the
aqueous-phase, the hydrate reverts back to the volatile carbonyl form of the aldehyde. Virtually
100% of the formaldehyde present in aqueous solution exists as methanediol. This is not the
case for other aldehydes. For example, only 58% of acetaldehyde present in aqueous solution
exists in the hydrate form.3 In the presence of ultraviolet irradiation the hydroxyl radical
abstracts a hydrogen atom from methanediol to form the hydrated formyl radical. The hydrate
then reacts with dissolved oxygen to produce formic acid. Free radicals abstract a hydrogen
atom from formic acid to produce formate, which then further reacts to carbon dioxide.
EXPERIMENTAL
A set of two FEP teflon tubes, each containing four milliliters, of solution was used in every

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irradiation experiment. Both tubes contained three milliliters of an aqueous formaldehyde
solution equivalent to 240 nanomoles. In addition, one tube also contained one milliliter of
deionized water and was designated as the experimental control. The other contained one
milliliter of hydrogen peroxide solution equivalent to 1000 nanomoles. Each irradiation
experiment was conducted for time periods of from one to four hours. After irradiation, the
samples were analyzed for formaldehyde and formate. Two additional four milliliter samples
were also prepared prior to each irradiation experiment. These were similar in composition to
the irradiated solutions, and were analyzed immediately for formaldehyde and formate. These
results were used for the values of the irradiation control samples.
A li-Cor spectroradiometer was used to measure the irradiance of the UVB lamp at a
wavelength of 313 nanometers. The output of the lamp was then compared to the irradiance of
ambient sunlight at the same wavelength. This analysis determined that solutions in the Teflon
tubes were exposed to a radiant energy of 0.005 calorie/sq cm., while irradiance from the sun
on a cloudless day is 0.004 calorie/sq cm. The contents of the tubes were maintained to within
two degrees of ambient temperature by a stream of compressed air which impinged upon the
exterior of the tubes during irradiation.
The reagents used in these experiments were obtained from a commercial scientific supply
source. The 37% formaldehyde and 3% hydrogen peroxide solutions were volumetrically
titrated against standard iodine and potassium permanganate solutions respectively. Deionized
water used in the experiment was obtained from a laboratory water filtration system which
contained a five micron prefilter, activated carbon absorption, and deionization using a mixed
bed ion exchange resin.
ANALYTICAL
All formaldehyde samples were prepared for analysis by reacting one milliliter of sample
with one milliliter of a 2.4-Dinitrophenylhydrazine/Acetonitrile solution at ambient temperature
for a period of ten minutes. The DNPH prepared from highly purified crystals, converts the
formaldehyde to the corresponding hydrazone derivative. HPLC standards were prepared from
pure crystals of formaldehyde hydrazone. Analysis were performed in the gradient mode using
a Varian Model 5000 HPLC equipped with a Zorbax C18 reverse phase column. A Dionex UV
detector operating at a wavelength of 360 nanometers was used for quantitation.
Formate samples were analyzed on the Ion Chromatograph within one hour after collection
to preclude losses in solution. Standards were prepared daily, and standard additions were
performed to verify formate concentrations. Anion analysis was carried out in the isocratic
mode using a Dionex Model 4000 Ion Chromatograph equipped with an IonPac AS-5 separator
column, A conductivity detector was used for quantitation.
discussion
The lower line in Figure 2 shows the decrease in formaldehyde concentration resulting from
the irradiation of solutions initially containing 240 nanomoles of formaldehyde and 1000
nanomoles of hydrogen peroxide. Formaldehyde continues to decrease in concentration up to
three hours of irradiation. After that time the concentration stays relatively constant.
Each data point on the upper line represents the results of two separate sets of experiments.
Solutions in both sets contain formaldehyde and deionized water. In addition, samples in one
set also contain 500 nanomoles of sulfate anion and the other solutions contain 500 nanomoles
of nitrate anion. The anions were added as the sodium salts.
The middle graph, like the one above it, also represents two separate sets of irradiation
experiments.

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Solutions in both sets were treated in the same manner as the ones just discussed. The
difference, however, is that these solutions contain hydrogen peroxide. At a specified
irradiation time, compare the differences in formaldehyde concentration between solutions in
the lower two curves against concentrations depicted by the controls in the upper plot. At one
hour irradiation, seventeen percent and twenty-four percent is photo-oxidized. At two hours
irradiation, the removal is thirty percent and forty percent. Three hours irradiation removes
thirty percent and fifty percent. After three hours irradiation, the formaldehyde removed is
thirty percent and seventy percent.
The inorganic anions are scavenging hydroxyl radicals causing a decrease in the removal of
formaldehyde by photo-oxidation. The decrease is dependent upon the concentrations of
hydrogen peroxide, formaldehyde, and inorganic anions. It demonstrates that the photo-
oxidation of formaldehyde in the aqueous-phase is reduced by the presence of either sulfate or
nitrate anions.
The formyl radical, produced from the photo-oxidation of formaldehyde reacts with
dissolved oxygen to produce formic acid. The formic acid is then photo-oxidized to formate ion.
Aqueous solutions of formaldehyde and hydrogen peroxide were saturated with either air or
oxygen. The solutions were then irradiated for periods of from one to four hours. As Figure 3
indicates, production of formate in air saturated aqueous solutions reaches a maximum
concentration, then slowly diminishes as the available hydroxyl radicals are depleted, In oxygen
saturated solutions, the initial formate concentration is slightly lower than that occurring in air
saturated solutions. However, later on it achieves a greater maximum before decreasing back
to a concentration similar to that obtained in air saturated solutions. McElroy and Waygood4
performed steady-state irradiation experiments with oxygen saturated aqueous solutions of
hydrogen peroxide and formaldehyde. They too observed an initial inhibition of formate
production followed by an increase in formate concentration. It appears that hydroxyl radicals
first consume the dissolved oxygen present in solution before photo-oxidizing formic acid to
produce formate ions.
Figure 4 illustrates the effect that inorganic anions have upon formate ion production- The
solutions in all three sets of experiments contain 240 nanomoles of formaldehyde and 1000
nanomoles of hydrogen peroxide. In addition, one set contains 500 nanomoles of nitrate anion
and the other solutions contain 500 nanomoles of sulfate anion. Both anions were added as the
sodium salt
Within the irradiation interval between thirty minutes and two hours, formate production
increases with length of irradiation. Also formate production is described by three separate
rates of reaction. Several investigators conclude that nitrate and sulfate radicals are produced
from the corresponding anions in aqueous solution. This occurs either by direct absorption of
radiant energy or through reactions with hydroxyl radicals. Neta and Huie5 measured the
reaction of nitrate, sulfate, and hydroxyl radicals with aqueous formic acid. They experimentally
measured the rate constants < 1.0X10% 1.4X106, 1.5X1CP of these reactions to be respectively.
After two hours irradiation, the formate production due to hydroxyl radicals decreases because
of depletion of those radicals. However, formate production due to sulfate and nitrate radicals
continues to increase. This increase is dependent upon the concentrations of anion and
hydrogen peroxide. However radicals photolytically produced from inorganic anions result in an
increase in formate ion production.
CONCLUSION
Formaldehyde is ubiquitous in the atmosphere. It is the precursor of formic acid which is
responsible for a significant portion of the acidity found in global precipitation. Its unique
ability to exist in the liquid-phase as a stable hydrate, would indicate that clouds, fogs, and dew
are the ultimate sinks for removal of formaldehide from the atmosphere. However, if the

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atmospheric aqueous-phase evaporates before hydrated formaldehyde is photo-oxidized, then
the remaining hydrate reverts back to the volatile carbonyl form of the compound. Data
indicates that in the aqueous-phase either sulfate or nitrate solutions reduce the concentration
of available hydroxyl radicals, thereby inhibiting the photo-oxidation of formaldehyde.
Sulfate and nitrate anions also increase the aqueous-phase formate ion concentration
resulting from the photo-oxidation of formaldehyde. In so doing, the likelihood of
recombination of formate and hydrogen ions to produce volatile formic acid is also increased.
All of these conditions considered together indicate that the aqueous-phase of the
atmosphere is not the ultimate sink for gas-phase formaldehyde. Furthermore, the atmospheric
aqueous-phase may even function as a source for gas-phase formic acid.

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H
H— C	+ HOH <	> H—C—OH
j
OH
H
H —C—OH + : OH 	> H— C—OH
313 nm
OH
OH
H- C— OH	„
I	+ 02 —» H02 + h— Q
O
OH
\
OH
/?
H- C
OH
313nm
-* HCOO +
H
Figure 1. Reactions of formaldehyde in aqueous
solution.
300
0
>
1
<
EC
o
LL
CO
UJ
—I
O
I>
z
<
z
200
100
HCHO/H20/SO4
HCHO/H20/NO3
HCHO/H202/SO4
HCHO/H202/NO3
HCHO/H202
-1	r
112	3
HOURS OF IRRADIATION AT 313 NANOMETERS
Figure 2. Effects of inorganic anions upon
the photoreaction of formaldehyde.

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OXYGEN SATURATED
SOLUTION
60 -
50 -
40 -
A IK S VI I K VI KI)
SOLUTION
30
20 -
10 -
HOURS OF IRRADIATION AT 313 NANOMETERS
Figure 3. Effects of dissolved oxygen upon
formate ion production.
70
HCH0/H202/S04
60 -
HCHO/H202
50 -
40 -
30 -
HCH0/H202/N03
20 -
10 -
0
2
4
HOURS OF IRRADIATION AT 313 NANOMETERS
Figure 4. Effect of inorganic anions upon the
production of formate ion.

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References
Grosjean, D.Environmental Science Technology
198216 254-262.
Keene, W.C.; Galloway, J.N.; Holden, J.D.,
Journal of Geophysical Research 1983.28
5122-5130.
Bell; Clunie. Transactions Faraday Society 1952 48 439.
McElroy, J.W.; Waygood, S. J. Journal Chemical Society
Transactions 1991.81 1513-1521.
Neta, P.; Huie, R.E.; Journal Physical Chemistry 1986
jq 4644-4648.

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Disclaimer
The information in this document has been funded wholly by the United States
Environmental Protection Agency. It has been subjected to Agency review and approved for
publication. Mention of trade names or commercial products does not constitute endorsement
or recommendation for use.

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Key Words
Formaldehyde
Formic Acid
Hydroxyl Radicals
Photo-oxidation
Liquid Chromatography
Ion Chromatography

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TECHNICAL REPORT DATA
1. REPORT NO.
EPA/600/A-95/085
2.
3 .R
4. TITLE MID SUBTITLE
HETEROGENEOUS PHOTOREACTION OF FORMALDEHYDE WITH
HYDROXYL RADICALS
S.REPORT DATE
6.PERFORMING ORGANIZATION CODE
7. AUTHORCS)
Daniel Driscoll
8.PERFORMING ORGANIZATION REPORT
NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Heterogeneous Chemistry and Aerosol Research Branch
National Exposure Research Laboratory
Research Triangle Park, NC 27711
10.PROGRAM ELEMENT NO.
11. CONTRACT/GRANT NO.
12. SPONSORING AGENCY NAME AND ADDRESS
National Exposure Research Laboratory
Office of Research and Development
U.S. Environmental Protection Agency
Research Triangle Park, NC 27711
13,TYPE OF REPORT AND PERIOD
COVERED
14. SPONSORING AGENCY CODE
15. SUPPLEMENTARY NOTES
16. ABSTRACT
Atmospheric heterogeneous photoreactions occur between formaldehyde and
hydroxyl radicals to produce formic acid. These photoreactions not only occur in
clouds, but also in other tropospheric hydrometeors such as precipitation and dew
droplets. Experiments were performed by irradiating Teflon tubes containing
nanomole concentrations of formaldehyde and hydrogen peroxide. Following
irradiation at a wavelength of 313 nanometers, the concentrations of formaldehyde
and formate ion were analyzed using High Pressure Liquid Chromatography and Ion
Chromatography. Data are presented to illustrate how nitrate and sulfate anions
effect the photo-oxidation of formaldehyde in the aqueous-phase, as well as the
effects of these anions on the formate ion which is a product of this photo-
oxidation. Formic acid and its precursor formaldehyde are soluble in the aqueous-
phase of the atmosphere. However, inhibition of the atmospheric photo-oxidation
reactions of these compounds coupled with evaporation of the clouds or dew droplets
can result in recycling the compounds from the liquid-phase back to the gas-phase
of the atmosphere.
17. KEY WORDS AND DOCUMENT ANALYSIS
a. DESCRIPTORS
b.IDENTIFIERS/ OPEN ENDED
TERMS
C.COSATI
Formaldehyde
Formic Acid
Hydroxyl Radicals
Photo-oxidation
Liquid Chromatography
Ion Chromatography



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