&EPA
United States	EPA-600/R-04/144
Environmental Protection	„ . .
Agency	October 2004
Evaluation of Total
Organic Emissions
Analysis Methods


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EPA-600/R-04/144
October 2004
Evaluation of Total Organic Emissions
Analysis Methods
By
Raymond G. Merrill
Eastern Research Group, Inc.
1600 Perimeter Park Drive
Morrisville, NC 27560
Contract 68-D7-0001
Purchase Order 2C-R212-NALX
Project Officer: Jeffrey V. Ryan
National Risk Management Research Laboratory
Air Pollution Prevention and Control Division
Air Pollution Technology Branch
U.S. Environmental Protection Agency
Research Triangle Park, NC 27711
U.S. Environmental Protection Agency
Office of Research and Development
Washingtion, DC 20460

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Abstract
The rationale and supporting experimental data for revising EPA's 1996 Guidancefor Total
Organics are summarized in this document, which reports both the results of research and the
investigation of improvements to the Total Organic Emissions (TOE) guidance used by EPA
to measure recoverable organic material from stationary source emission samples in support of
the Office of Solid Waste Risk Burn requirements. This document describes the purpose,
experimental design, and results from several related investigations into the performance of
specific techniques to determine TOE. Results include analysis of recoverable organic material
from three specific boiling point/vapor pressure classes: light hydrocarbons and volatile
organics, semivolatile organics, and nonvolatile organic compounds. Improved procedures for
analysis of volatile organics, semivolatile organics, and nonvolatile organic compounds are
described. The experimental approach used to address weaknesses in TOE analysis procedures
is discussed, and the effect of improvements to these measurement procedures is reported. The
experimental results in this report support the sampling and analytical guidance necessary to
characterize the full range of recoverable organic material encountered in source emissions.
11

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Foreword
The U.S. Environmental Protection Agency (EPA) is charged by Congress with protecting
the Nation's land, air, and water resources. Under a mandate of national environmental laws,
the Agency strives to formulate and implement actions leading to a compatible balance
between human activities and the ability of natural systems to support and nurture life. To meet
this mandate, EPA's research program is providing data and technical support for solving
environmental problems today and building a science knowledge base necessary to manage
our ecological resources wisely, understand how pollutants affect our health, and prevent or
reduce environmental risks in the future.
The National Risk Management Research Laboratory (NRMRL) is the Agency's center for
investigation of technological and management approaches for preventing and reducing risks
from pollution that threaten human health and the environment. The focus of the Laboratory's
research program is on methods and their cost-effectiveness for prevention and control of
pollution to air, land, water, and subsurface resources; protection of water quality in public
water systems; remediation of contaminated sites, sediments and ground water; prevention
and control of indoor air pollution; and restoration of ecosystems. NRMRL collaborates with
both public and private sector partners to foster technologies that reduce the cost of
compliance and to anticipate emerging problems. NRMRL's research provides solutions to
environmental problems by: developing and promoting technologies that protect and improve
the environment; advancing scientific and engineering information to support regulatory and
policy decisions; and providing the technical support and information transfer to ensure
implementation of environmental regulations and strategies at the national, state, and
community levels.
This publication has been produced as part of the Laboratory's strategic long-term research
plan. It is published and made available by EPA's Office of Research and Development to
assist the user community and to link researchers with their clients.
Sally Gutierrez, Acting Director
National Risk Management Research Laboratory
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EPA Review Notice
This report has been peer and administratively reviewed by the U.S. Environmental Protection
Agency and approved for publication. Mention of trade names or commercial products does not
constitute endorsement or recommendation for use.
This document is available to the public through the National Technical Information Service,
Springfield, Virginia 22161.
iv

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Contents
Section	Page
Abstract	ii
List of Figures	 vi
List of Tables 	 vi
Acronyms and Abbreviations	vii
Acknowledgments 	viii
1	Introduction 	1
2	Conclusions and Recommendations	3
3	Methods and Materials	5
Total Volatile Organic Emissions	5
Analysis Recommendations and QC Requirements	5
Sample Analysis	7
XAD-2 Cleaning Methods 	7
Method 0010 Sample Recovery and Preparation	9
Total Chromatographable Organics	9
Nonvolatile (GRAV) Organics	11
4	Experimental Procedures 	13
Measurement of Volatile Organic Emissions 	13
Evaluation Experiments 	13
FGC Calibration Performance 	13
Instrument Detection Limit Study 	15
Procedures to Evaluate XAD-2 Preparation and Blanking 	16
Procedures for Evaluating the Artifacts and Interferences with GRAV Analysis .... 17
Fine Particulate Filtration Evaluation	18
Filter Extract Inorganic Interference Evaluation 	18
Probe Wash Inorganic Interference Evaluation 	19
5	Quality Control Procedures 	21
QC for Volatile Organic Analysis by FGC	21
XAD-2 QA/QC	21
QC for Total Chromatographable Organics	22
QC Requirements for GRAV	22
6	References	24
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List of Figures
Figure	Page
1	Stationary Source Emissions Sampling and Analysis Techniques	 2
2	Schematic of Solid Sorbent Extractor	 9
3	Modified Method 3452 Sample Preparation Scheme for Method 0010
Components Analyzed for TCO and GRAV	 10
4	Packed Column FGC Calibration Chromatogram	 15
List of Tables
Table	Page
1	Field GC Analysis Recommendations and QC Requirements 	 5
2	Calibration Standard Specifications	 6
3	FGC Chromatographic Conditions 	 14
4	Calibration Response Factors 	 14
5	FGC Detection Limits 	 15
6	XAD-2 Blank Results 	 16
7	XAD-2 GRAV Blanks	 17
8	Inorganic Salts Mixture Used for GRAV Interferents Analysis 	 18
9	Filtration Simulation, Glass Wool versus Cellulose Filtration	 18
10	Filter Extraction Simulation, Methylene Chloride Extracts	 19
11	Probe Rinse Simulation, Methanol/Methylene Chloride Extracts	 19
12	Field GC Analysis QC Requirements 	 21
13	QC Guidelines for XAD-2 Resin Preparation	 22
14	QC Requirements for Total Chromatographable Organics	 22
15	QC Requirements for GRAV Analysis of Volatile Compounds	 23
vi

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Acronyms and Abbreviations
Term
Definition
AEERL
BP
C7
C10
C12
C14
c17
ch4
DSCM
EPA
FGC
FID
GC
GMW
GRAV
IDL
Level 1
MDL
MS
NERL
nonvolatile
ORD
ppmv
osw
QC
RCRA
ROP
RTP
SCOT
SVOCs
TCO
Tedlar
TOE
VOCs
EPA's former Air and Energy Engineering Research Laboratory
boiling point
//-heptane (straight chain hydrocarbon, saturated, 7 carbon atoms)
«-decane
«-dodecane
//-tetradecane
//-heptadecane (straight chain hydrocarbon, saturated, 17 carbon atoms)
methane
dry standard cubic meter
U.S. Environmental Protection Agency
field gas chromatography
flame ionization detector
gas chromatograph or gas chromatography
gram molecular weight
gravimetric mass
instrument detection limit
AEERL Procedures Manual: Level 1 Environmental Assessment
minimum detection limit
mass spectrometry
EPA's National Exposure Research Laboratory
compound class generally defined by boiling point above 300 °C
EPA's Office of Research and Development
parts per million by volume
EPA's Office of Solid Waste
quality control
Resource Conservation and Recovery Act
recommended operating procedure
Research Triangle Park
surface coated open tubular chromatographic column
semivolatile organic compounds
total chromatographable organic compounds
trade name for sampling bag material used in direct collection of air samples
total organic emissions (combination of FGC, TCO, and GRAV mass)
volatile organic compounds
Vll

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Acknowledgments
This document was prepared for the U.S. Environmental Protection Agency's Office of
Research and Development, NRMRL/APPCD/Air Pollution Technology Branch, located in
Research Triangle Park, NC. Technical contributions from Jeffrey Ryan at EPA and from
Dr. Joan Bursey and Mr. Robert Martz of Eastern Research Group are gratefully acknowledged.
viii

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Section 1
Introduction
The EPA's Office of Solid Waste (OSW) has devel-
oped guidance1 for conducting stack emissions tests
in association with requirements for the permitting of
hazardous waste combustion facilities. The guidance
for conducting a risk burn requires a comprehensive
characterization of organic and inorganic emissions,
including the determination of total organic emissions
(TOE). Stand alone guidance for determining TOE
was previously released in 1996.2 The TOE determi-
nation represents a gross measurement of the total
volatile, semivolatile, and nonvolatile organic com-
pounds emitted. The TOE are used to derive an
organic mass balance to qualify the completeness and
uncertainty of the associated risk assessment.
Peer and public reviews of OSW's Risk Burn Guid-
ance for Hazardous Waste Combustion Facilities
were critical of the TOE methodology, particularly
with respect to the total chromatographable organic
compound (TCO) and gravimetric mass (GRAV)
measurement procedures used to characterize the
semivolatile and nonvolatile organic fractions respec-
tively. Significant concern was raised regarding the
potential for high bias in the GRAV determination
due to the presence of inorganic compounds in the
TOE sample. Combined with the GRAV method's
lack of measurement sensitivity relative to the non-
volatile, organic analyte-specific measurement
methods, the GRAV portion may dominate the
overall TOE measurement, resulting in a poor quanti-
tative characterization of the identified compounds.
As a result of these comments and concerns, a series
of experiments were conducted to investigate identi-
fied measurement issues and achieve procedural
improvements in order to ultimately revise and
update the TOE methodology. This document de-
scribes the purpose, experimental design and results
from several related investigations into the perfor-
mance of specific techniques to determine TOE.
The procedures described in this report measure and
report TOE mass. These procedures are performed to
permit comparison of the portion of organic emis-
sions that have been characterized by "target analyte
specific" methods to the total organic emissions from
source sampling.
TOE procedures allow the total volatile and extract-
able organic mass from stationary source emissions
to be quantified. The total recoverable organic mass
reported as TOE is the result of combining data from
three fractions of organic compounds: volatile or-
ganic compounds (VOCs), TCO for semivolatile
organic compounds, and GRAV measurements for
nonvolatile organic material. These three fractions
are defined as follows:
•	VOCs include organic compounds with boil-
ing points less than 100 °C. These compounds
are measured by two analytical techniques.
Gas samples collected by EPA SW-846
Method 00403 or equivalent are analyzed
using a field gas chromatography (FGC).
Volatile organic materials captured in the
condensate of the EPA SW-846 Method 0040
train are analyzed using purge and trap gas
chromatography/flame ionization detection
(GC/FID);
•	Semivolatile organic compounds (SVOCs)
include organic material with boiling points
between 100 and 300 °C. SVOCs are mea-
sured by TCO analysis performed by an
1

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Evaluation of Total Organic
analytical laboratory using GC/FID and
sample extract(s) from an EPA SW-846
Method 00104 sampling train; and
Nonvolatile organic compounds (GRAV)
with boiling point greater than 300 °C are
measured as the residual mass of sample
extract(s) from a Method 0010 sampling train.
The combination of two sampling and four analytical
techniques gives the investigator the gross mass of all
recoverable organic material. The total organic
emissions are the sum of VOCs, TCO, and GRAV.
A summary of these three techniques is shown in
Figure 1.
Total Stationary Source Emissions
&^s><
Volatile Organics
BP < 100 °C
Sampling:
Method 0040
Analysis:
Field GC/FID for Bag;
Purge & Trap GC/FID for
Condensate
Semivolatile Organics
BP 100 - 300 °C
Sampling:
Method 0010
Preparation:
Method 3542
Analysis:
TCO Using GC/FID
Nonvolatile Organics
BP > 300 °C
Sampling:
Method 0010
Preparation:
Use Semivolatile Organic
Sample Extract
Analysis:
GRAV, Weigh Residual
Organic Material
Figure 1. Stationary Source Emissions Sampling and
Analysis Techniques.
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Emissions Analysis Methods
Section 2
Conclusions and Recommendations
Conclusions
Results of the research performed on synthetic and
authentic environmental samples focused on issues
ranging from the availability of calibration standards
for field GC analysis to determining the cause for
inorganic interference with the nonvolatile organic
analysis.
Volatile organic compounds (BP<100 °C) present in
samples collected in Tedlar bags according to the
procedures in EPA SW-846 Method 00 403 require
analysis in the field by GC/FID (FGC). The recovery
of gaseous standard material containing methane,
ethane, propane, //-butane, //-pentane, //-hexane, and
w-heptane was confirmed. Polymer-based packed
columns such as Alltech Haysep Q 80/100 mesh and
surface coated open tubular (SCOT) gas chromatog-
raphy columns such as J&W GS-Q or equivalent
were found to provide adequate resolution and
sensitivity for this analysis. The minimum detection
limit (MDL) of FGC is limited by the amount of
sample that can be introduced through the sample
loop. The MDL that can be achieved by FGC is in the
range of 0.6 ppm (1500 |ig/m3). For stationary source
samples with volatile organic material present at or
below 1 ppm, the FGC method is not sensitive
enough to provide a reliable basis for volatile TOE
unless samples are concentrated using alternative
techniques. Where FGC shows results less than
1 ppm, independent laboratory analysis of the bag
samples for methane should be performed followed
by cryogenic preconcentration and analysis of C2 - C7
organic compounds. For volatile compounds col-
lected in bags, uniform FID response for all com-
pound classes is assumed in this methodology.
Determination of SVOC mass in samples collected by
EPA SW-846 Method 00104 requires extraction and
concentration of the various train components as
described in EPA SW-846 Method 3542s and subse-
quent analysis by GC/FID. Field samples collected in
this way require additional preparation to remove
polar volatile solvents (methanol or acetone), inor-
ganic interferents, and water prior to concentration.
Experimental results demonstrate that the performing
the separatory funnel extraction of the XAD-2 and
particulate filter Soxhlet extracts followed by 0.45
|im filtering was adequate to remove the field recov-
ery solvents, inorganic interferents, and water. The
separatory funnel extraction of the Soxhlet extracts
represents a minor operational modification to the
Method 3 542 sample preparation approach but offers
significant improvement in analytical results. Experi-
mental results also showed that XAD-2 porous
polymer resin used to collect SVOCs can be cleaned
with an extraction procedure using water, methylene
chloride, and methanol.
Nonvolatile organic material analysis is also per-
formed for the Method 0010 train components. The
same sample extract used to determine SVOCs is
used to determine nonvolatile organic materials.
Evaporation of a known volume of the Method 0010
organic extract has resulted in variable and artificially
high results because of improper sample preparation
and inorganic interferents retained during sample
preparation. The experimental results from GRAV
analysis of synthetic and authentic combustion
particulate sample extracts showed that the separatory
funnel extraction of the XAD-2 and particulate filter
Soxhlet extracts followed by 0.45 |im filtering was
adequate to remove more than 90% of the potential
inorganic interferences to gravimetric determination
of nonvolatile organic material in Method 0010
3

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Evaluation of Total Organic
samples. Methanol solvent wash and methylene
chloride extraction of solid particulate from munici-
pal waste combustion fly ash and a cement kiln dust
showed the presence of dissolved inorganic materials.
The separatory funnel extraction of these Soxhlet
extracts followed by 0.45 |im filtering was adequate
to reduce the level of inorganic interference below
detection limit for the GRAV measurement. As a
result, the modifications to Method 3542 to include
the separatory funnel extraction of the XAD-2 and
particulate filter Soxhlet extracts followed by 0.45
|im filtering specifically for TCO and GRAV analysis
will be included in the revised TOE guidance. Addi-
tional experimental results indicated the need for
precautions to monitor and minimize airborne dust
depositing in GRAV samples. GRAV mass of partic-
ulate extracts was so low in the two cases studied that
deposition of room air dust could make a significant
contribution to the total mass of GRAV samples.
GRAV analysis samples must be covered using
procedures described in the method so that airborne
dust is minimized as an interference. Finally, GRAV
analysis results indicated a need to perform analysis
in duplicate and report the average result to achieve
the required accuracy.
Recommendations
FGC procedures should be modified to require
calibration with a gaseous hydrocarbon standard
containing methane, ethane, //-propane, //-butane, //-
pentane, //-hexane, and //-heptane. Concentrations of
individual hydrocarbons and the total hydrocarbon
balance should be calculated in units of |ig/m\ Total
volatile organic material measured from Method
0040 bag samples at concentrations less than 1500
|ig/m3 are likely to be below detection limits, and
samples should be concentrated for accurate compari-
son to speciated volatile compound analysis of the
same stationary source emissions.
Packed column analysis should be performed with a
minimum of 1 mL gaseous injection. SCOT column
analysis should be performed at an injection volume
that provides the optimum peak shape without split-
ting the methane elution profile (peak).
XAD-2 must be cleaned within 2 weeks of use or
reevaluated for blank content requirements for TCO
and GRAV. Preparation of XAD-2 requires cleaning
to the minimum standards reported here which
includes washing with water, extraction with metha-
nol, and extraction with methylene chloride, de-
scribed elsewhere in this report.
For TCO and GRAV analysis, Method 0010 samples
must follow the modified Method 3542 preparation
procedures. Method 3542 does not remove all of the
potential inorganic interference in methylene chloride
extracts of the mixture of inorganic salts studied in
this evaluation. Following soxhelt extraction, the
XAD-2 and particulate filter methylene chloride
extracts should be extracted at least twice with
adequate volumes of both high and low pH water
followed by 0.45 |im filtering to ensure complete
removal of potential inorganic interferences in source
samples. Either glass wool or cellulose fiber filters
may be used in the final drying step prior to concen-
tration of the extract.
The entire analysis window for TCO is established by
injecting w-heptane (C7) and «-heptadecane (C17) as
the retention time reference peaks between which the
TCO integration will occur. The retention time
window for TCO is established during calibration.
The TCO range is defined by all peaks falling after C7
(//-heptane) and before C17 (//-heptadecane). Integra-
tion of the detector response must begin after the C7
returns to within 10% of baseline, and terminate
when the beginning (front) of C17 is more than 10%
of baseline.
GRAV analysis must be performed on a balance of at
least 5 place accuracy (10 |ig detection limit). GRAV
analysis must be performed in duplicate and reported
as the average of the two measurements. Samples
must be protected from deposition of room air dust to
achieve the quality control (QC) limits shown in this
report.
4

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Emissions Analysis Methods
Section 3
Methods and Materials
Total Volatile Organic Emissions
Compounds with boiling points below 100 °C are
sampled into Tedlar bags using EPA SW-846 Meth-
od 0040 sampling procedures and analyzed in the
field by GC/FID. The GC/FID recommended operat-
ing procedure (ROP) for this method is found in
Guidance for Total Organics 2 The range of applica-
ble compounds for total VOC determination includes
methane, with a boiling point of -160 °C, to «-hep-
tane, with a boiling point of 98 °C. The nominal
reporting range for the methodology extends to 100
°C. Methane, ethane, and propane can be separated
and reported individually. The FGC results are
reported as a total.
Analysis Recommendations and QC Require-
ments
Calibration standards can be ordered at several
concentrations or prepared by dilution of a certified
stock standard in Tedlar bags or pressurized
cylinders. A dilution of a certified C, - C7 standard
gas mixture should also be prepared as a daily quality
control calibration check sample. This QC sample
should be prepared at a concentration approximately
in the middle of the calibration range and should be
analyzed at least once per day during stationary
source field sampling. QC requirements for field GC
analysis of volatile compounds are shown in Table 1.
A custom certified calibration gas standard pur-
chased commercially that contained methane, ethane,
//-propane, //-butane, //-pentane, //-hexane, and //-
heptane at approximately 100 ppm each in nitrogen
was used for all work on FGC work reported here.
The concentration of each hydrocarbon and of the
total mixture was calculated in micrograms per cubic
meter as described later in this section. The stock
standard contained compounds shown in Table 2.
Table 1. Field GC Analysis Recommendations and QC Requirements.
Analysis Recommendations
Instrumental Parameter	Recommended Value
Injection Temperature
Detector Temperature
Initial Column Temperature
Column Oven Temperature Program
Final Column Oven Hold Parameters
Packed Chromatographic Column Capable of C,
Normal Hydrocarbon Separation
SCOT Chromatographic Column Capable of C, -
mal Hydrocarbon Separation.
C7 Nor-
Ambient
220 °C
100 °c
100 °C to 190 °C at 15 °C/min
Hold at 190 °C for 12 min
HaysepQ 80/100 mesh, 2 m by 3.18 mm by 2.16 mm
ID stainless steel (or equivalent) (Alltech)
GS-Q, 30M by 0.53 mm ID fused silica J&W Scientific
(or equivalent)
continued
5

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Evaluation of Total Organic
Table 1. (concluded)
QC Requirements
Quality Indicator
Performance Requirements
Peak Resolution
Calibration Materials
Calibration Curve
Sensitivity
Precision
Bias
Completeness
Retention Time
R = 1.252
Certified gas cylinder(s) containing methane, ethane,
propane, butane, pentane, hexane, and heptane
Three concentrations that bracket the sample analy-
sis range.
• Correlation coefficient of 0.995 or greater
Single calibration measurements should agree
within 20% of the average calibration curve or a
mean response factor.
5 jig/dry standard cubic meter (DSCM)
±15% relative standard deviation on replicate analysis.
±10% relative standard deviation on daily QC analyses.
Fresh QC check samples are prepared if analysis fails
QC check requirements. If subsequent analysis results
also fail QC requirements, instrument is recalibrated.
100% of the aliquots are reanalyzed if analysis calibra-
tion check results do not meet quality specifications.
±5% relative standard deviation from mean retention
time for each calibration compound.
Table 2. Calibration Standard Specifications.
Compound
Concentration (ppm)
Concentration (jig/m3)
Methane
103
67,700
Ethane
105
129,000
^-Propane
104
188,000
/7-Butane
105
250,000
/7-Pentane
101
299,000
«-Hexane
101
357,000
^-Heptane
101
415,000
Total
720
1,706,000
6

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Emissions Analysis Methods
The concentration of each hydrocarbon in parts per
million was converted to micrograms per cubic meter
using
C

= c„
ppm
GMW
24.414
x 1000
(1)
where:
C , 3
flg/r-
c,
ppm
= concentration of each hydrocarbon
expressed in micrograms per cubic
meter;
= concentration of each hydrocarbon
expressed in parts per million;
GMW = gram molecular weight of each hydro-
carbon.
The following steps were applied to each component
of the multi-component standard:
1.	Determine the concentration of each compo-
nent of the standard in parts per million.
2.	Using the equation above, convert the con-
centration of each component to micrograms
per cubic meter.
3.	Sum the concentrations of each of the compo-
nents in micrograms per cubic meter to obtain
a total concentration, which can then be
related to the sum of the chromatographic
peak areas at each concentration level.
To determine total mass of the hydrocarbons for the
calibration curve, the concentrations of each of the
hydrocarbons (in micrograms per cubic meter) are
summed, and total concentration (sum of the concen-
tration of each component of the standard) is plotted
vs. area counts. Calibration values for individual
hydrocarbons and the sum of the entire group were
determined using linear regression and average
response calculations.
A Varian Model 3400 gas chromatograph equipped
with a HaysepQ 80/100 mesh, packed chromato-
graphic column measuring 2 m by 3.18 mm by 2.16
mm ID stainless steel and a FID was configured to
perform VOC analysis.
Gas was introduced into the injection port of the gas
chromatograph using a 1 mL stainless steel gas
sampling loop. A SCOT column coated with Porapak
QS was also evaluated for FGC analysis. An example
of column(s), conditions, and QC requirements is
shown in Table 1. The chromatographic column(s)
used in the field GC must be capable of resolving the
Cx - C7 hydrocarbons at baseline level. A multipoint
calibration consisting of at least three points (ana-
lyzed in triplicate) at different concentrations was
prepared.
Sample Analysis
After calibration of the field GC, sample analysis
begins when the sample container (the Tedlar bag) is
connected to the sampling valve and the sample gas
is drawn through the valve and into the GC sample
loop or a gas-tight syringe. The sample is injected
into the chromatograph. The temperature program
and integrator/data system data acquisition are started
simultaneously with the injection of the sample.
Chromatograms and integrator/data system output are
collected.
The sample is analyzed twice (i.e., duplicate injec-
tions) and integrated from a retention time of zero to
the end of the C7 peak. The total concentration in
micrograms per cubic meter is calculated from the
summed integrated area of the standard peaks from
zero through the end of C7 to yield the value for the
gaseous portion of the TOE analysis for each injec-
tion.
The simple arithmetic mean of the duplicate injec-
tions is reported as the bag portion of the FGC
results.
XAD-2 Cleaning Methods
XAD-2 is a macroreticulate porous polymer made
from styrene (vinylbenzene) and divinylbenzene. The
emulsion block copolymerization process used to
form cross-links in the resin gives the resin a pore
structure and chemical stability ideally suited for
sampling and recovery of SVOCs from the Method
0010 sampling train. The polymer synthesis process
also exposes the raw resin to high concentrations of
7

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Evaluation of Total Organic
naphthalene, styrene (vinylbenzene), divinylbenzene
and low molecular weight byproducts of these re-
agents and the polymerization reaction. If these com-
ponents are not removed during XAD-2 cleaning,
they will be extracted as part of the sample prepara-
tion procedures and will provide a positive bias to the
TCO results. It is essential that XAD-2 be clean and
free from contaminants that could contribute a
positive bias to the TCO and GRAV determination.
Preparation of XAD-2 within 2 weeks of sampling or
experimental work provides sufficient time for the
cleaning and drying process and avoids extended
storage, which may result in contamination and
elevated levels of extractable material in blanks. The
cleaning method described below has proven to be a
cost-effective and high quality procedure for prepar-
ing XAD-2.
The procedure for cleaning XAD-2 is derived from
the Procedures Manual: Level 1 Environmental
Assessment (SecondEdition)6 developed by the U.S.
EPA. The original methodology has been improved
to provide a reproducible method for preparing
sorbent material sufficiently clean for low level
organic compound capture and analysis. The com-
plete cleaning cycle requires approximately 5 work-
ing days to finish. Typical background or blank total
organic concentrations (TCO) from XAD-2 prepared
by this procedure are on the order of 1 |ig per gram of
sorbent medium. Typical GRAV background after
cleaning by this procedure is on the order of 6 |ig per
gram of sorbent medium. The cleaning procedure
used to obtain these results includes the following
steps:
Resin is obtained from the manufacturer,
supplier or recycled from prior use. Resin
cleaned by a secondary vendor should be
treated like recycled resin. Recycled and
recleaned resin usually contains less organic
contamination and is preferred over raw
material straight from the manufacturer.
Naphthalene is the most common contami-
nant in sorbent that has not been properly
cleaned.
The resin is soaked and washed several times
with deionized water if new from the manu-
facturer. Resin "fines" float to the surface of
the water wash and are removed by skimming
the surface with a fine screen or dipping the
fines out with a glass beaker before the next
cleaning step.
The water-washed or recycled resin is loaded
directly into the extractor for solvent clean-
ing. The entire cleaning procedure is done
"wet" with final drying taking place only at
the end of the process.
•	Distillation type extractors, each capable of
holding 900 g of resin, are typically used to
extract the resin with sequential extractions of
methanol, methylene chloride, and methylene
chloride again. An extraction apparatus fol-
lowing the design in Appendix B of the Pro-
cedures Manual: Level 1 Environmental
Assessment (Second Edition)6 and shown in
Figure 2 is efficient and effective for XAD-2
cleaning. Solvent sufficient to completely fill
the extraction chamber and at least 30% of
the solvent reservoir is required for the ex-
traction. Fresh solvent is used for each step of
the extraction. Solvent is drained between
steps, and the extractor is pre-rinsed with the
solvent to be used in the next step.
•	After the final extraction, the methylene
chloride is drained and the extractor body is
removed to a hood where the resin is dried.
Drying is accomplished by a gentle stream of
nitrogen. The nitrogen is delivered from the
gas output of a liquid nitrogen tank through a
heat exchanger to the sorbent to remove
methylene chloride residue from the final
extraction step. Residual methylene chloride
is reduced to 1000 |ig/g of resin.
The dried resin is transferred to a clean, dry
glass jar with a Teflon or equivalent lined
screw cap lid. For blank QC purposes, a
portion of the dried sorbent equal to a typical
field sample (usually 40 g) is extracted, pre-
pared and analyzed by the method used for
field samples.
If the analysis of the clean XAD-2 meets
method acceptance criteria, it is labeled with
8

-------
Emissions Analysis Methods
Condenser
Condenser
XAD-2 Extractor
Glass Frit
Glass Tube
Distilling/Moisture
Test Receiver
Heavy Walled
Teflon Tubing
3 Ball Snyder
Columns
Round Bottom
with Side Arm
Figure 2. Schematic of Solid Sorbent Extractor.
a laboratory identification number and stored
at room temperature in a clean, solvent-free
cabinet for use in sampling. The clean resin is
stable and can be stored for 2 to 3 weeks.
Longer storage times are possible if the mate-
rial is refrigerated, but a blank sample must
be checked before material stored for longer
than 3 weeks is used for field sampling.
Method 0010 Sample Recovery and
Preparation
Stationary source samples for application of this
analysis method are taken by SW- 846 Method 00104
and prepared by SW-846 Method 3542s (as modified
for TCO and GRAV analysis). A flow chart for
preparation of SW-846 Method 0010 samples using
modified SW-846 Method 3542 is shown in Figure 3.
The Method 0010 sampling train generates three
sample fractions from various train components that
must be carefully recovered and prepared to ensure
results that meet the quality requirements for TCO
and GRAV analysis. Solvents used to rinse sampling
train glassware may dissolve inorganic material and
water. The inorganic material is carried through the
sample preparation process with the water and polar
solvents and remains in the concentrated extracts
unless steps are taken to remove the water and polar
solvents. The interferents must be removed during the
sample extraction and preparation steps before the
organic extract is concentrated for final analysis.
Several minor modifications were made to Method
3542 to ensure that water soluble (polar) solvents and
inorganic material are removed from the samples
prior to TOE analysis. At the end of the preparation
procedures, extracts may be combined and concen-
trated to a final volume of 5 mL, producing one
extract per Method 0010 sampling train for the TOE
analysis. Alternatively, each of the three sample
extracts may be analyzed for TOE separately.
Total Chromatographable Organics
TCO analysis is used to measure the total organic
mass of compounds boiling between 100 and 300 °C.
The TCO Method is described in detail in Guidance
for Total Organic2 The TCO procedure consists of
analysis of the combined concentrated probe wash,
9

-------
(Q
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Q. 10
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SI
o» -T
3 (D
Q.T3
° 3
4
Soxhlet Extraction
CH2CI2
Spike with
Method-8270 Surrogates
Optional
Combine CH2CI2/CH3PH
rinse with XAD-2 Extract
Back half of Filter Holder,
Connector, and Condenser Rinse
CH2CI2/CH3OH)
	 (Container 5)	
Add Sufficient Water to
Separate into Two Phases;
Extract Water Layer with CH2CI2
Adjust PH and do Base/Neutral
& Acid Extraction
Concentrate to 5 mL	<
Filter w/ 0.45 um Teflon Filter	SI
Analyze for TCO and GRAV
Modified 3542 for TOE	H
w
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3
o'

-------
Emissions Analysis Methods
filter, XAD-2, and impinger extracts from the three
major components of the sampling train. The analysis
is generally performed in the laboratory after extrac-
tion, compositing, filtration, and concentration of the
extracts of the individual components of the
Method 0010 sampling train.
Capillary chromatography is used to obtain the best
possible resolution of chromatographic peaks. For
stationary source emission gas samples, an aliquot of
the Method 0010 methylene chloride extract is
injected onto a capillary GC column with an FID
detector, and the peak areas are summed over the
retention time window that encompasses the TCO
boiling point range. The entire analysis window is
established by inj ecting //-heptane and //-heptadecane
as the retention time reference peaks between which
the TCO integration will occur. The retention time
window for TCO is established during calibration.
The TCO range is defined by all peaks falling after C7
and before C17. Integration of the detector response
begins after the C7 peak returns to within 10% of
baseline and terminates when the beginning (front) of
the C17 peak is more than 10% of baseline.
The TCO calibration standard curve is generated with
hydrocarbon standards that fall within the TCO
range, specifically «-decane (C10), «-dodecane (C12),
and //-tetradecane (C14). Calibration is performed
using a solution of standards prepared from neat
liquid standards of the individual hydrocarbons in
methylene chloride. The quantitative calibration
standards should be prepared to cover the concentra-
tion range expected in the source samples. A multi-
point calibration of at least three points (in triplicate)
is generated in units of micrograms per milliliter. The
calibration curve for TCO is calculated using linear
regression statistics. The total detector response for
the TCO range reported as one number is used in the
linear regression calculation. The C7 and C17 detector
response is not included for the calibration point
determination. After calibration has been performed,
a daily QC check sample is run to verify that the GC
is performing correctly. The QC check sample
consists of a standard in the middle of the working
range of the GC calibration standards.
Nonvolatile (GRAV) Organics
The third component of the total organics measure-
ment process is called gravimetric mass (GRAV).
The GRAV method has also been described in detail
in Guidance for Total Organics2 Extractable organic
materials with nominal boiling points of 300 °C and
higher are determined by this procedure. Samples
extracted from sampling media using methylene
chloride and dried to constant weight are determined
quantitatively by this procedure. GRAV analysis
measures organic compounds with vapor pressures
less than or equal to //-heptadecane (C17). The range
of organics is defined by boiling point, in this case
greater than 300 °C.
For stationary source emission gas samples, the
GRAV procedure is carried out on an aliquot of the
same Method 0010 methylene chloride extract used
for TCO determinations. GRAV analysis is per-
formed using samples prepared using the modified
SW-846 Method 3542 procedures to remove both
inorganic interferents and water from the sampling
train recovery process. The analysis is generally
performed in the laboratory after extraction, composi-
ting, filtering, and concentrating the extracts of the
individual components of the Method 0010 sampling
train.
The GRAV method quantifies organic material with
a boiling point greater than 300 °C. A carefully
measured aliquot of the Method 0010 methylene
chloride extract is placed in a cleaned, dry, pre-
weighed aluminum weighing pan. The solvent is
allowed to evaporate in a fume hood at room temper-
ature. Exposure to dust and contaminants is mini-
mized by covering the samples with an aluminum
tent or placing them under some form of clean cover.
The GRAV samples are then dried completely to
constant weight in a room temperature desiccator.
The residue in the pan is weighed accurately, and the
mass is recorded as the GRAV value. For this proce-
dure, a portion of the pooled extracts from the
Method 0010 sampling train used for TCO are also
used for GRAV. A volume of 1 mL of the pooled
extract is used for each of the GRAV determinations.
Duplicate GRAV analyses are performed, and the
11

-------
Evaluation of Total Organic
results are averaged.
GRAV organics with BP greater than 300 °C are
measured on an analytical balance capable of weigh-
ing accurately to ±0.005 mg (5 |ig). The GRAV
value, in micrograms, is converted to units of
micrograms per sample by multiplying the average
pan results for the sample by the ratio of total extract
volume to GRAV aliquot volume. Final GRAV
results are reported in micrograms per cubic meter by
dividing micrograms per sample by the Method 0010
sample volume (expressed as cubic meters).
12

-------
Emissions Analysis Methods
Section 4
Experimental Procedures
Measurement of Volatile Organic Emis-
sions
The Volatile Organic Emissions portion of the Total
Organic Emissions measurement is determined in two
parts:
•	First, a field GC analysis of the gaseous
portion of the Method 00 403 sample is con-
ducted using sample collected in a Tedlar
bag. The range of organic material identified
by field GC is defined by the boiling point
range less than 100 °C. The procedure is
normally performed in the field to minimize
sample (compound) loss due to storage and
shipping.
•	Second, the aqueous portion of the sample
(condensate from the Method 0040 sampling
train) is analyzed in the laboratory using
purge and trap GC with a FID. This aqueous
portion, which is actually the condensate from
Method 0040 sample, is normally transferred
to a vial with no headspace for shipment to
the laboratory.
Field GC analysis is limited by a detection limit of
1-10 parts per million by volume (ppmv), equiva-
lent to 2000 - 20,000 |ig/m3. Identification of meth-
ane, ethane, and propane is possible although specific
compound identification is not required for the TOE
determination. These compounds may be present in
significant quantities in stack samples, and correct
identification and quantification will more accurately
characterize the organic emissions.
Once both portions have been quantified, they are
added together to yield the volatile organic emissions
contribution to the TOE.
Evaluation Experiments
A series of laboratory experiments was completed to
determine the quality parameters and detection limits
for the FGC method. The goal of the FGC evaluation
experiments was to confirm that calibration standards
that include //-heptane could be prepared and ana-
lyzed using the FGC analytical procedure. A second
goal of the FGC evaluation was determination of
detection limits for samples collected in Tedlar bags.
Previous FGC experiments were performed with n-
hexane as the latest-eluting compound in the calibra-
tion gas. Prior to these evaluation experiments, the
volatile organic material in the boiling point range
between //-hexane and//-heptane was erroneously not
included in the FGC results. (Note that the TCO
determination begins after elution of the C7 peak,
whereas the FGC determination includes the C7
peak).
The experimental work included evaluation of a
commercially prepared standard containing normal
hydrocarbons from methane to //-heptane and a
detection limit study for the method.
Experiments were performed to:
•	Confirm that w-heptane could be included in
the calibration mixture;
•	Determine the response factor for individual
calibration compounds and the average re-
sponse factor for the C, - C7 calibration
mixture; and
•	Evaluate both a polymer based packed gas
chromatographic column and a SCOT column
for FGC analysis.
FGC Calibration Performance
The stock standard was diluted into reusable 1.5-L
13

-------
Evaluation of Total Organic
high pressure aluminum cylinders. The concentra-
tions of all hydrocarbons in calibration standards
were 74,500 |ig/m3 (31 ppm); 117,000 |ig/m3 (51
ppm); 337,000 |ig/m3 (148 ppm); 845,000 |ig/m3
(371 ppm); and 1,706,000 |ig/m3 (720 ppm). The
FGC was calibrated by injecting each of these cali-
bration samples into the gas chromatograph three
times. A linear regression was performed on the
resulting peak area versus concentration data. Cali-
bration response factors in units of area per concen-
tration were determined from the linear regression
statistics of the calibration sample analysis results.
Gas chromatographic conditions used to generate
calibration response factors and detection limits are
shown in Table 3. Response factors for individual
hydrocarbons and the total VOC response factor are
shown in Table 4. An example packed column
chromatogram showing peak shape, resolution, and
retention times is presented in Figure 4.
Table 3. FGC Chromatographic Conditions.
FGC Instrument Parameter	Instrument Set Point
Injector Temperature
Ambient
Initial Column Temperature
o
o
o
O
Column Oven Temperature Program
100 °C to 190 °C at 15 °C/min
Final Column Oven Hold Parameters
Hold at 190 °C for 12 min
Column Carrier Gas Flow
Approximately 30 mL/min
FID Detector Temperature
220 °C
Table 4. Calibration Response Factors.
Linear Regression
Compound
Response Factor3
Correlation Coefficient (r2)
Methane
5.0575
0.9987
Ethane
5.611
0.99995
Propane
5.6159
0.99995
«-Butane
5.7466
0.99996
/7-Pentane
5.6923
0.99993
«-Hexane
5.749
0.99993
^-Heptane
5.6889
0.99998
Average
5.6783
0.99957
a Varian GC/FID Model 3400
14

-------
Emissions Analysis Methods
Figure 4. Packed Column FGC Calibration Chromatogram.
Other choices of polymer-based column packing may
give satisfactory results but should be evaluated
before use. Most polymer-based columns capable of
separating methane, ethane, and propane suffer from
breakdown and bleed at the temperatures needed to
elute //-heptane.
Instrument Detection Limit Study
The lowest calibration standard concentration (i.e.,
74,500 |ig/m3, or 31 ppm) was used to determine an
approximate instrument detection limit for this
method under the operating conditions described in
Table 3. The detection limit for individual com-
pounds C, through C7 was calculated using the
approach in EPA 40 CFR 136, Appendix B. Results
of the instrument detection limit (IDL) for individual
components and for the sum of the compounds in the
calibration standard are shown in Table 5. The
approximate instrument detection limit was deter-
mined for each of the calibration components and for
the sum of the components because the detection
limit will vary depending on the composition of the
sample. The lowest calibration point was not within
2 to 5 times the estimated detection limit as specified
by 40 CFR Part 136, Appendix B. Precision for fixed
loop injections was very high resulting in a minimum
detection limit range (on a per compound basis) of
100 to 200 |ig/m3. FGC instrument detection limits
can be as high as 1400 |ig/m3 (0.6 ppm) for the sum
of the calibration standard components due to the
chromatographic data system imprecision integrating
the broad peaks for C6 and C7 hydrocarbons.
Table 5. FGC Detection Limits.

IDL
Std.
Compound
(Hg/m3)
Deviation
Methane
106
33.8
Ethane
106
33.9
Propane
167
53.4
Butane
219
69.8
/7-Pentane
270
86.1
/7-Hexane
460
147
^-Heptane
1112
353
Total
1440
459
15

-------
Evaluation of Total Organic
Procedures to Evaluate XAD-2 Prepa-
ration and Blanking
Prior to sampling, the sampling train and sampling
media must be prepared, cleaned and blanked. Al-
though some forms of "precleaned" resin are com-
mercially available, all XAD-2 used for TCO and
GRAV analysis must be analyzed before use to
ensure the extractable background of semivolatile and
nonvolatile organic materials meets the appropriate
quality requirements. Many unexpectedly high TCO
and GRAV field sample results originate from poor
preparation and cleaning of sampling train or XAD-2
sampling media. The experimental procedures pro-
vided in this section demonstrate that XAD-2 can be
cleaned to meet quality control requirements for TCO
and GRAV analysis.
Experiments were performed to evaluate the ability to
clean XAD-2 to acceptable levels for use as a solid
sorbent for TOE measurements and to establish the
quality control requirements for properly cleaned
XAD-2 sampling media. The cleaning requirements,
including acceptable blank levels for TCO and
GRAV determination, are included in this section.
Untreated XAD-2 is available from the manufacturer,
Supelco, Inc.; precleaned XAD-2 is available from
various commercial vendors. All XAD-2 used for
TCO and GRAV analysis, recleaned just prior to use
or not, must be checked for TCO and GRAV blank
levels. The quality control check for XAD-2 cleanli-
ness requires extracting a portion of the sorbent
approximately equal to the quantity that will be used
to collect samples (typically 20 to 40 g). If TCO and
GRAV blank levels do not meet the method quality
control requirements, the XAD-2 should be re-
cleaned. XAD-2 should be prepared within 2 weeks
of the sampling episode and can be stored at room
temperature in a clean glass bottle closed by a Teflon
lined cap. Refrigerated storage is acceptable and may
retard increases in blank contamination.
In the experimental work described here, TCO
analysis was performed on portions of XAD-2
sorbent media cleaned according to the procedures
described in Section 3. XAD-2 sorbent was then
checked for residual TCO and GRAV. Two lots of
approximately 1000 g of XAD-2 were cleaned. One
lot of XAD-2 was untreated (raw) resin directly from
the manufacturer. A second lot was prepared using
recycled XAD-2 that had been cleaned and stored dry
in a clean glass jar with Teflon lined screw cap for
over 6 months. Each lot of XAD-2 was extracted and
dried as it would be for a source sampling episode.
Two (2) 40-g portions of XAD-2 from each lot were
extracted with pesticide grade methylene chloride in
a Soxhlet extractor to determine the residual TCO
and GRAV in the cleaned resin. TCO and GRAV
were determined for both lots of XAD-2. Blank
aliquots were concentrated using a 3-ball Snyder
column. Final concentrates were never taken below
3.0 mL. Final sample volume used for TCO and
GRAV analysis was 5 mL. The TCO and GRAV
analysis results from these two lots of XAD-2 are
shown in Table 6.
Table 6. XAD-2 Blank Results.

TCO
GRAV
LotDescription
(M-g/g)
(ng/g)
New XAD-2
0.975
5.94
New XAD-2 (Duplicate)
NAa
5.62
Used XAD-2
0.985
6.56
Used XAD-2 (Duplicate)
NA
NA
"NA = not available.
A second issue associated with residual organic
material in clean XAD-2 involves the effect of
preparing XAD-2 extracts using EPA SW-846
Method 3542s separatory funnel extraction proce-
dures. The question investigated was whether prepar-
ing methylene chloride extracts using Method 3542
increases, decreases, or has no effect on the back-
ground organic material in the clean resin. Therefore,
two sets of XAD-2 blank extracts were prepared. The
first set was extracted with methylene chloride and
dried by filtration through anhydrous sodium sulfate.
The second set of extracts was processed through
SW-846 Method 3542 separatory funnel extraction
procedures. The results of these experiments are
shown in Table 7. The practical detection limit for
GRAV is 6.6 |ig/gram XAD-2 based on a balance
16

-------
Emissions Analysis Methods
Table 7. XAD-2 GRAV Blanks.
Average
mg GRAV/ \ig GRAV/ \ig GRAV/
Sample Description
mg/mL"
40 g XAD-2
gm XAD-2
gm XAD-
Recycled XAD, Rep 1 without Method 3542 preparation
0.090
0.45
11.
& &
Recycled XAD, Rep 2 without Method 3542 preparation
0.015
0.08
2.0
o.o
New XAD, Repl without Method 3542 preparation
0.025
0.12
3.0
^ Q
New XAD, Rep2 without Method 3542 preparation
0.070
0.35
8.8
j y
Recycled XAD, after Method 3542 Preparation Rep 1
0.045
0.22
5.5

Recycled XAD, after Method 3542 Preparation, Rep 2
0.005
0.02
0.5
2.4
Recycled XAD, after Method 3542 Preparation, Rep 3
0.010
0.05
1.2

New XAD, after Method 3542 Preparation, Rep 1
0.015
0.08
2.0

New XAD, after Method 3542 Preparation, Rep 2
0.025
0.12
3.0
2.7
New XAD, after Method 3542 Preparation, Rep 3
0.025
0.12
3.0

a milligrams GRAV per milliliter extract. Extracts were taken to a final volume of 5 mL; 1-mL aliquot used to perform GRAV determination.
sensitivity of 10 |ig. Results in Table 7 have been
reported using 0.01 mg as the lower limit of weight
for a 10|i balance. Using Method 3542 provides an
added step that reduces the blank GRAV component
of the XAD-2 sorbent material and is more represen-
tative of the blank contribution to SW-846 Method
0010 samples prepared using SW-846 Method 3542.
Procedures for Evaluating the Artifacts
and Interferences with GRAV Analysis
Artifacts and interferences in GRAV measurements
have been reported by several investigators,7 produc-
tion of GRAV artifacts from inorganic carryover
during sample preparation is one of the most com-
monly reported concerns. Common interferents
include
•	Fine particles, which may be collected during
sampling of stationary source emissions or
may be due to XAD-2 fragments;
•	Methanol, collected during sampling or a
residual of the solvents used in the field to
rinse the sampling train components;
•	Water, used in the sampling train or collected
during sampling because of the moisture
content of the stationary source; and
• Inorganic salts collected during sampling.
These materials must be removed from sample
extracts prior to sample concentration and TCO and
GRAV analysis. Improper filtering of the sample
extracts can result in GRAV analyses biased high.
Inadequate removal of methanol and/or water results
in loss of TCO material. Inadequate removal of
methanol and/or water also causes a positive bias in
GRAV results due to carryover of inorganic salts.
Inorganic salts can also be extracted from filter
samples by methylene chloride. Inorganic salts must
be removed from the sampling train extracts prior to
concentration to avoid a positive bias in GRAV.
Methanol and water must also be removed from the
organic extracts of the Method 0010 samples prior to
any concentration step so that the only solvent pres-
ent during evaporative concentration is methylene
chloride (BP 49 °C). The presence of water and/or
methanol in the final extract will result in a higher
boiling solvent during the sample concentration step.
This higher boiling mixture will cause a loss of
semivolatile organic material in the TCO boiling
point range.
17

-------
Evaluation of Total Organic
Experiments were designed to investigate these issues
as well as to determine if modifications to EPA SW-
846 Method 3542 would mitigate the commonly re-
ported interferences to the TCO and GRAV analyses.
Authentic fly ash samples from a municipal waste
incinerator and from a cement kiln were used to
represent a typical field sample. Environmental fly
ash and cement kiln dust were extracted both individ-
ually and in a 50:50 mixture. Mixtures of 10 inor-
ganic salts as shown in Table 8 were also prepared by
weighing equal portions of each salt into a high
density linear polyethylene container and mixing
together thoroughly. Experimental work was per-
formed using recovery solvents specified in EPA
SW-846 Method 0010 and extraction solvents speci-
fied in EPA Method 3542. The solid fly ash and
synthetic inorganic mixture were extracted separately
with several solvents typical of source sample recov-
ery to determine whether inorganic interference
occurred in standard sample preparation procedures
and whether separatory funnel extraction followed by
0.45 |im filtering mitigated those interferences.
Table 8. Inorganic Salts Mixture Used for GRAV
Interferents Analysis.
Aluminum Chloride
Calcium Chloride
Calcium Chloride Dihydrate
Calcium Sulfate Hemihydrate
Calcium Sulfate Dihydrate
Ferric Chloride
Iron(II) Sulfate Heptahydrate
Lead (II) Chloride
Potassium Chloride
Potassium Sulfate
Sodium Nitrate
Zinc Chloride
Zinc Sulfate
Fine Particulate Filtration Evaluation
Cement kiln dust was extracted with methylene
chloride for 16 hours in a Soxhlet extractor. Two
procedures were performed on the sample extract.
First, the sample was filtered through 1 g of anhy-
drous sodium sulfate supported by a glass wool plug
as specified in EPA Method 3542. The resulting
liquid filtrate was concentrated to 5 mL and analyzed
for nonvolatile mass using the GRAV technique. In
a second procedure the glass wool plug was replaced
by a cellulose filter that was folded and placed into
the glass filtration funnel. One gram of anhydrous
sodium sulfate was added to the filter paper in the
funnel, and the sample extract was filtered through
this apparatus. The results of glass wool plug versus
cellulose filter filtration, shown in Table 9, indicate
there is no difference between the two approaches to
remove fine particulate from methylene chloride
particulate extracts.
Table 9. Filtration Simulation, Glass Wool versus
Cellulose Filtration.
Cellulose
Glass Wool	Filter
Sample	jig/gram par- jig/gram par-
Description3	ticulate	ticulate
Sample 1	120	120
Sample 2	185	210
a Cement kiln dust methylene chloride extract
Filter Extract Inorganic Interference Evalua-
tion
Methylene chloride was used to extract particulate
material and a mixture of inorganic salts to test
whether samples prepared using SW-846 Method
0010 contained materials that interfere with the
GRAV methodology. Potential interferents include
the presence of dissolved solids or fine particulates.
The purpose of extracting real and synthetic particu-
late material was to determine whether inorganic salts
could be extracted with methylene chloride and
produce an interference or artifact in the GRAV
analysis procedure.
The inorganic salt mixture was extracted in a Soxhlet
extractor using 100% methylene chloride for 16
hours. The mixture of inorganic salts was extracted in
18

-------
Emissions Analysis Methods
duplicate. Methylene chloride extraction was fol-
lowed by drying with anhydrous sodium sulfate,
filtration through cellulose filter media, and Kuderna
Danish concentration to 5 mL. Replicate GRAV
analysis was performed on each 5-mL extract. The
results of these analyses are shown in Table 10,
where GRAV results as high as 1900 |ig/gram of salt
mixture are observed. A separate set of extracts of the
inorganic salt mixture was also prepared. Water was
added to this second set of methylene chloride ex-
tracts and extracted using the separatory funnel
liquid/liquid extraction procedures described in SW-
846 Method 3542. These extracts were prepared
following Method 3542, dried by filtration through
anhydrous sodium sulfate, filtered using a 0.45 |im
Teflon syringe filter, and concentrated to 5 mL.
Treatment of these samples using the EPA Method
3542 separatory funnel liquid/liquid extraction and
0.45 |im filtering resulted in a significant decrease of
the inorganic interferences. The results of the salt
mixture extraction and Method 3542 preparation are
shown in Table 10. Results from the extraction of the
salt mixture show that interference with the GRAV
analysis is possible if inorganic salts are present in
source emission samples and that separatory funnel
liquid/liquid extraction and 0.45 |im filtering removes
most of the dissolved inorganic interference.
Table 10. Filter Extraction Simulation, Methylene
Chloride Extracts.




GRAV in Ex-

GRAV in
tract after

Extract
Method-3542
Sample Description
Hg/gm solid
Cleanup
Salts Mixture
1962
309
Salts Soxhlet Duplicate
1650
368
Cement Kiln Dust
110
175
Cement Kiln Dust,
215
220
Duplicate


Municipal Waste Fly
190
200
Ash (Extract 1)


A similar treatment of fly ash and cement kiln dust
(Table 10) indicates that organic GRAV material is
present on the real fly ash and is not affected by the
separatory funnel liquid/liquid extraction and 0.45
|im filtering cleanup.
Probe Wash Inorganic Interference Evalua-
tion
To evaluate the potential inorganic interference from
Method 0010 probe washes, the mixture of inorganic
salts was added to a solution of methylene chloride
saturated with methanol. The mixture contained 20%
methanol in methylene chloride. Five (5) grams of
salt mixture were added to the methanol/methylene
chloride solvent, thoroughly mixed and allowed to
stand for 30 minutes. The same treatment was per-
formed on a mixture of cement kiln dust (2.5 g) and
municipal waste combustor fly ash (2.5 g).
Soluble salts and organic material were recovered by
filtering the supernatant liquid from these extracts.
GRAV analysis was performed on the soluble frac-
tion. The results are shown in Table 11. Samples
were further processed using SW-846 Method 3542
separatory funnel liquid/liquid extraction and 0.45
|im filtering. The results of the GRAV analysis of the
Method 3542 preparations are also shown in Table
11. The GRAV results from methanol/methylene
chloride extracts of cement kiln dust and municipal
waste combustor fly ash were approximately 50 times
higher in mass than the comparable methylene
chloride sample extracts. Greater than 90% of the
potential interference was removed by processing the
samples with the separatory funnel liquid/liquid
extraction and 0.45 |im filtering.
Table 11. Probe Rinse Simulation, Meth-
anol/Methylene Chloride Extracts.
GRAV in Ex-
GRAV in tract after
Extract Method 3542
Sample Description ng/gm solid Cleanup
Mixed Salts	584,400	2016
50/50 Mixed MWC	10,900	100
Ash/Cement Kiln Dust
19

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Evaluation of Total Organic
20

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Emissions Analysis Methods
Section 5
Quality Control Procedures
QC results for TOE measurements are summarized in
this section.
QC for Volatile Organic Analysis by
FGC
QC for FGC analysis is performed during calibration
and daily QC check sample analysis. QA/QC require-
ments for FGC method evaluation are shown in Table
12.
XAD-2 QA/QC
Analytical interferences or contaminants appear in
the resin after storage. These contaminants may cause
the cleaned resin to fail the QC requirements for the
method used in sample analysis. Contaminants
originate from both external contamination or oxida-
tion and from internal "bleeding" of entrained chemi-
cals from very small or inaccessible pores in the
resin. Subsequent recleaning and reuse reduces the
internal contributions to blank during storage. Conse-
quently, cleaned XAD-2 has a usable shelf life of 1
month after cleaning unless the sorbent is refriger-
ated.
Contaminant levels may also increase if XAD-2 is
exposed to high concentrations of oxidizing agents
such as ozone or oxides of nitrogen during sampling.
In an oxidizing environment, oxidation or decomposi-
tion products of XAD-2 such as naphthalene, benzoic
acid, benzaldehyde, aromatic esters, carboxylic acids,
and aldehydes will be observed. Sufficiently high
levels of NOx (mole percent levels) can cause de-
struction of the resin itself.
Cleaned XAD-2 resin was checked for blank contam-
ination by extracting a quantity of resin equivalent to
the amount to be used during sampling (typically
40 g). The resin was prepared and analyzed using the
Table 12. Field GC Analysis QC Requirements.
Quality Indicator	Requirements
Peak Resolution (R)
R= 1.252.
Calibration Materials
Certified gas cylinder(s) containing methane, ethane, propane, butane,

pentane, hexane, and heptane.
Calibration Curve
Three concentrations that bracket the sample analysis range.

Correlation coefficient of 0.995.

Single calibration measurements should agree within 20% of the average

calibration curve or a mean response factor.
Sensitivity
1500 (ig/dry standard cubic meter (DSCM).
Precision
±15% relative standard deviation on replicate analysis.
Bias
±10% relative standard deviation on daily QC analyses. Fresh QC check

samples are prepared if analysis fails QC check requirements. If subsequent

analysis results also fail QC requirements, instrument is recalibrated.
21

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Evaluation of Total Organic
same volumes of solvent and preparation procedures
that are used for field samples following the proce-
dures in EPA SW-846 Method 3 542 for Method 0010
samples. Extracts were analyzed for TCO and GRAV
and must meet the QC criteria in Table 13 in order for
the XAD-2 to be acceptable for sampling. If the
extracted resin fails to meet the acceptance criteria,
the resin should be recleaned by sequential Soxhlet
extractions (once each) with methanol and methylene
chloride. A sample of resin failing after a recleaning
should be discarded, and no further attempts made to
clean that batch.
Table 13. QC Guidelines for XAD-2 Resin
Preparation.
Maximum	XAD-2
Blank (per (used in this
Analysis gram of Resin) evaluation)
TCO	1 |ig/g resin 0.98 |ig/g resin
GRAV	10 |ig/g resin 5.9 to 6.4 |i/g
QC for Total Chromatographable
Organics
Quality control for TCO analysis by GC/FID is
performed during calibration and daily QC check
sample analysis. QA/QC requirements and perfor-
mance experienced during TCO method evaluation
are shown in Table 14. All QA/QC for TCO analysis
is performed external to field sample analysis.
QC Requirements for GRAV
The predominant interferences for GRAV are incom-
plete cleaning of XAD-2, airborne dust deposited
during weighing procedures, and inadequate control
of environmental conditions in weighing facilities.
Blank weighing pans without solvent or sample were
carried through the evaporation and drying process as
a quality control check for each set of samples.
Sample weights were not corrected for blank weigh-
ing pan mass gain. Solvent blank samples consisting
of concentrated reagent solvent should be analyzed in
duplicate for each batch of samples. GRAV results
were not corrected for solvent blanks. Therefore, the
GRAV results represent a worst case for possible
blank contamination.
The QC requirements for GRAV include calibration
of the analytical balance, use of Class A volumetric
glassware, duplicate analysis of each sample, and
weighing samples to constant weight ±10 |ig. QC
requirements for GRAV analysis of volatile com-
pounds are shown in Table 15. Performance targets
Table 14. QC Requirements for Total Chromatographable Organics.
Quality Indicator
Requirements
Compound Resolution
Calibration Curve
Sensitivity
Precision
Bias
Completeness
Retention Time
separation (a ) = 1.25.
Three concentrations that bracket the sample analysis range.
Correlation coefficient of 0.995.
No single calibration point deviation more than 20% from the average
calibration curve.
5 |ig/mL in solution.
±15% relative standard deviation on replicate analysis.
±10% relative standard deviation on daily QC analyses. Fresh QC check
samples are prepared if analysis fails QC check requirements. If subsequent
analysis results also fail QC requirements, instrument is recalibrated.
100% of the aliquots are reanalyzed if analysis calibration check results do not
meet quality specifications.
±5% relative standard deviation from the mean retention time for each
compound.	
22

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Emissions Analysis Methods	
Table 15. QC Requirements for GRAV Analysis of Volatile Compounds.
Quality Indicator	Requirements
Analytical Balance Sensitivity	± 0.01 mg (±10 |_ig).
Analytical Balance Calibration	Annually by NIST Traceable Standards.
Analytical Balance Calibration Checks	Daily during analysis periods using NIST Class S weights.
Precision	±15% relative standard deviation on replicate analysis or ±15 (.ig,
whichever is greater.
Bias	±5% relative standard deviation on daily QC check. If bias criteria
are failed, analytical balance is recalibrated.
Completeness	100%; aliquots are reanalyzed if analysis calibration check results
do not meet quality specifications.
Blank GRAV Pan	<50 |_ig
Reagent Blank	<60 i-ig
have been established from experimental laboratory
data.6
Inorganic salts and contamination of samples by
microfragments of XAD-2 and other fine particulate
matter can cause artifacts or interference in GRAV
analyses if the procedures described in this guidance
document are not followed. The use of Method 3542
with the additional procedures described in Section 4
to prepare samples collected from Method 0010 air
samples reduces the contamination. Either filtration
with prerinsed cellulose filter media (Whatman #1
filter media or equivalent) and drying with sodium
sulfate or filtration through anhydrous sodium sulfate
supported by a glass wool plug followed by 0.45 |im
filtering is effective at removing fine particle artifacts
that interfere with GRAV analysis.
The following steps are crucial to quality control for
GRAV analysis:
• Use high quality reagents for performing
procedures (extractions, rinses, etc.) "Ultra-
pure" reagents are recommended.
•	Assure that all glassware and field and labo-
ratory equipment have been cleaned thor-
oughly with high quality reagents. Cover the
weighing pan containing GRAV analysis
aliquot for drying by building a tent with
aluminum foil, shiny side out.
•	Allow solvent in GRAV pans to evaporate to
dryness before placing GRAV pans in a
desiccator for final drying to constant weight.
•	Run control pans: dry pan blank (dust blank)
and solvent blank. Blank weighing pans
without solvent or sample should be carried
through the evaporation and drying process as
a quality control check for each set of sam-
ples. Solvent blank samples consisting of
concentrated reagent solvent should be ana-
lyzed in duplicate for each batch of samples.
Sample weights should be corrected for blank
weighing pan mass gain using the dust blank.
•	Check balance calibration prior to each
weighing.
23

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Section 6
References
1.	U.S. EPA, Risk Burn Guidance for Hazardous Waste Combustion Facilities, EPA530-R-01-001. Office
of Solid Waste, Washington, DC, July 2001.
2.
3.	U.S. EPA, Guidance for Total Organics, EPA600-R-96-036 (NTIS PB97-118533). National Exposure
Research Laboratory, Research Triangle Park, NC, November 1996.
4.	U.S. EPA, SW-846 Method 0040, "Sampling of Principal Organic Hazardous Constituents from
Combustion Sources Using Tedlar® Bags", http://www.epa.gov/epaoswer/hazwaste/test/pdfs/0040.pdf
(Accessed October 2004)
5.	U.S. EPA, SW-846 Method 0010, "Modified Method 5 Sampling Train".
http://www.epa.gov/epaoswer/hazwaste/test/pdfs/001Q.pdf (Accessed October 2004)
6.	U.S. EPA, SW-846 Method 3542, "Extraction of Semivolatile Analytes Collected Using Method 0010
(Modified Method 5 Sampling Train)".
http://www.epa.gov/epaoswer/hazwaste/test/pdfs/3542.pdf (Accessed October 2004)
7.	U.S. EPA, IERL-RTP Procedures Manual: Level 1 Environmental Assessment (Second Edition). EPA-
600/7-78-201 (NTIS PB-293 795), October 1978.
8.	Public comments on Guidance on Collection of Emissions Data to Support Site-Specific Risk Assessments
at Hazardous Waste Combustion Facilities (EPA 530-D-98-002), as reported in the RCRA Docket (F-
1998-GCEA-FFFFF).
http://docket.epa.gov/edkpub/do/EDKStaffCollectionDetailView7obiectIiN0b0007d48001eee6 (Accessed
October 2004)
24

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TECHNICAL REPORT DATA
(Please read Instructions on the reverse before completing)
1. REPORT NO.
EPA-600/R-04/144
2.
3. RECIPIENT'S ACCESSION NO.
4. TITLE AND SUBTITLE
Evaluation of Total Organic Emissions Analysis Methods
5. REPORT DATE
October 2004



6. PERFORMING ORGANIZATION CODE
7. AUTHORS
Raymond G. Merrill
8. PERFORMING ORGANIZATION REPORT NO.
9. PERFORMING ORGANIZATION NAME AND ADDRESS
Eastern Research Group, Inc.

10. PROGRAM ELEMENT NO.
1600 Perimeter Park Drive
Morrisville, NC 27560


11. CONTRACT/GRANT NO.
68-D7-000, 2C-R212-NALX
12. SPONSORING AGENCY NAME AND ADDRESS
U. S. EPA, Office of Research and Development

13. TYPE OF REPORT AND PERIOD COVERED
Final, 06/00-09/03
Air Pollution Prevention and Control Division
Research Triangle Park, North Carolina 27711

14. SPONSORING AGENCY CODE
EPA/600/13
15. SUPPLEMENTARY NOTES
The EPA Project Officer is Jeffrey V. Ryan, Mail Drop E305-01, phone (919) 541-1437, e-mail
ryan.jeff@epa.gov
16. ABSTRACT
The rationale and supporting experimental data for revising EPA's 1996 Guidance for Total Organics are
summarized in this document, which reports both the results of research and the investigation of
improvements to the Total Organic Emissions (TOE) guidance used by EPA to measure recoverable
organic material from stationary source emission samples in support of the Office of Solid Waste Risk Burn
requirements. This document describes the purpose, experimental design, and results from several related
investigations into the performance of specific techniques to determine TOE. Results include analysis of
recoverable organic material from three specific boiling point/vapor pressure classes: light hydrocarbons
and volatile organics, semivolatile organics, and nonvolatile organic compounds. Improved procedures for
analysis of volatile organics, semivolatile organics, and nonvolatile organic compounds are described. The
experimental approach used to address weaknesses in TOE analysis procedures is discussed, and the
effect of improvements to these measurement procedures is reported. The experimental results in this
report support the sampling and analytical guidance necessary to characterize the full range of recoverable
organic material encountered in source emissions.
17.
KEYWORDS AND DOCUMENT ANALYSIS


a. DESCRIPTORS
b. IDENTIFIERS/OPEN ENDED TERMS
c. COSATI Field/Group
Air Pollution
Emission
Measurement
Experimental Design
Organic Compounds
Pollution Control
Stationary Sources
13B
14G
14B
07C
18. DISTRIBUTION STATEMENT

19. SECURITY CLASS (This Report)
Unclassified
21. NO. OF PAGES
32
Release to Public

20. SECURITY CLASS (This Page)
Unclassified
22. PRICE
EPA Form 2220-1 (Rev. 4-77) PREVIOUS EDITION IS OBSOLETE
forms/admin/techrpt.frm 7/8/99 pad

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