&EPA Sr^'^on Analytical Methods Approved for Compliance Monitoring under the Ground Water Rule Analysis for the following contaminants shall be conducted in accordance with the methods in the following table, or their equivalent as determined by EPA. The methods and monitoring requirements for these contaminants are specified in 40 CFR 141.402(c)(2). Additional methods are listed in Appendix A to Subpart C of Part 141. The CFR is the legal reference for approved methods and takes precedent over this table. The table should accurately reflect the analytical methods information published in 40 CFR 141. §141.402(c)(2)- Analytical Methods (1) The time from sample collection to initiation of analysis may not exceed 30 hours. The ground water system is encouraged but is not required to hold samples below 10°C during transit. (2) Collection of a standard sample volume of at least 100 mL for fecal indicator analysis regardless of the fecal indicator or analytical method used (§141.402(b)(3)) (3) Analysis of all ground water source samples using one of the analytical methods listed in the §141.402(c)(2) of this section for the presence of E.coli, enterococci, or coliphage. The procedures must be conducted with the documents listed at §141.402(c)(2) or one of the alternative methods listed in Appendix A to Subpart C of Part 141. For Standard Methods Online, the year in which each method was approved by the Standard Methods Committee is designated by the last two digits following the hyphen in the method number. The methods listed are the only online versions of the method that maybe used. For vendor methods, the date of the method listed in §141.402(c)(2) or Appendix A to Subpart C of Part 141 is the date/version of the approved method. Laboratories should be careful to use only the approved versions of the methods as product packaging inserts may not be the same as the approved versions of the methods. (a) Laboratory certification- systems must have all compliance samples required under this subpart analyzed by a laboratory certified by EPA or a primacy state to analyze drinking water samples. The laboratory used by the system must be certified for each method (and associated contaminant(s)) used for compliance monitoring analyses under this rule. (b) Incorporate by reference - The methods required in §141.402(c)(2) are incorporated by reference into the regulation. Contaminant Escherichia coli - Procedure (following Lactose Fermentation Methods): Office of Water (MS - 4606 M) EPA 815-B-19-006 April 2019 ------- Page 2 of 8 Method Organization Reference Title/Source Date Notes 9221 F Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be used for E.coli testing step after use of Standard Methods 9221B, 9221D, 9222B, or 9222C 9221 F Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition Standard Methods 2012 EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be used for E.coli testing step after use of Standard Methods 9221B, 9221D, 9222B, or 9222C 9221 F Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 EC-MUG (Method 9221F) can be used for E.coli testing step after use of Standard Methods 9221B, 9221D, 9222B, or 9222C 9221 F-06 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 2006 EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be used for E.coli testing step after use of Standard Methods 9221B, 9221D, 9222B, or 9222C Contaminant Escherichia coli - Partition methods: Method Organization Reference Title/Source Date Notes 9222 G.lc(2) Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 EC-MUG (Method 9221F) or NA-MUG (Method 9222G) can be used for E.coli testing step after use of Standard Methods 9221B, 9221D, 9222B, or 9222C 9222 1 Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 NA-MUG (Method 92221) can be used for E.coli testing step after use of Standard Methods 9221B, 9221D, 9222B, or 9222C Contaminant Escherichia coli - membrane filtration methods: ------- Page 3 of 8 Method Organization Reference Title/Source Date Notes 1604 EPA Method 1604: Total Coliforms and Escherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (Ml Medium), September 2002 EPA Method 1604 2002 m- ColiBlue24®Test Hach Company Membrane Filtration Method m- ColiBlue24® Broth, Revision 2, August 17,1999 m-ColiBlue 24 Test 1999 9222 J Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 Chromocult EMD Millipore Chromocult® Coliform Agar Presence/Absence Membrane Filter Test Method for Detection and Identification of Coliform Bacteria and Escherichia coli for Finished Waters, November 2000, Version 1.0 Chromocult 2000 Contaminant Escherichia coli - enzyme substrate methods: Method Organization Reference Title/Source Date Notes 9223 B Colilert® Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 9223 B Colilert® Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition Standard Methods 2005 9223 B Colilert® Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition Standard Methods 2012 ------- Page 4 of 8 Method Organization Reference Title/Source Date Notes 9223 B Colilert® Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 9223 B-97 Colilert® Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 1997 9223 B-04 Colilert® Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2004 9223 B Colisure® Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 9223 B Colisure® Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition Standard Methods 2005 9223 B Colisure® Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition Standard Methods 2012 9223 B Colisure® Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 9223 B-97 Colisure® Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 1997 ------- Page 5 of 8 Method Organization Reference Title/Source Date Notes 9223 B-04 Colisure® Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2004 9223 B Colilert-18® Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 9223 B Colilert-18® Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition Standard Methods 2005 9223 B Colilert-18® Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition Standard Methods 2012 9223 B Colilert-18® Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 9223 B-97 Colilert-18® Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 1997 9223 B-04 Colilert-18® Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2004 ------- Page 6 of 8 Method Organization Reference Title/Source Date Notes E*Colite® Charm Sciences Charm E*Colite™ Presence/Absence Test for Detection and Identification of Coliform Bacteria and Escherichia coli in Drinking Water, January 9,1998 Charm E*Colite 1998 Readycult® EMD Millipore Readycult® Coliforms 100 Presence/Absence Test for Detection and Identification of Coliform Bacteria and Escherichia coli in Finished Waters, January 2007, Version 1.1 Readvcult 2007 Modified Colitag® CPI International, Inc. Modified Colitag™ Test Method for the Simultaneous Detection off. coli and other Total Coliforms in water (ATP D05- 0035), August 28, 2009 Modified Colitag 2009 Tecta EC/TC, v. 1.0 Veolia Water Solutions and Technologies Tecta™ EC/TC medium and the Tecta™ Instrument: A Presence/Absence Method for the Simultaneous Detection of Total Coliforms and Escherichia coli (E. coli) in Drinking Water, May 2014, Version 1.0 Tecta, Version 1.0 2014 Tecta EC/TC, v. 2.0 Pathogen Detection Systems, Inc. Tecta™ EC/TC medium and the Tecta™ Instrument: A Presence/Absence Method for the Simultaneous Detection of Total Coliforms and Escherichia coli (E. coli) in Drinking Water, March 2017, Version 2.0 Tecta, Version 2.0 Contaminant ------- Page 7 of 8 Enterococci - Mu tiple Tube Technique methods: Method Organization Reference Title/Source Date Notes 9230 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 9230 B-04 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 2004 Enterococci - membrane filtration methods: Method Organization Reference Title/Source Date Notes 9230 C Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 9230 C Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 1600 EPA EPA Method 1600: Enterococci in Water by Membrane Filtration Using membrane- Enterococcus Indoxyl-P-D- Glucoside Agar (mEI) EPA 821-R-02-022 September 2002 EPA Method 1600 (2002) 2002 Enterococci - enzyme substrate methods: ------- Page 8 of 8 Method Organization Reference Title/Source Date Notes Enterolert Applied and Environmental Microbiology Budnick, G.E., R.T. Howard, D.R. Mayo. 1996. "Evaluation of Enterolert for Enumeration of Enterococci in Recreation Waters". Applied and Environmental Microbiology. 62:3881-3884. 1996 9230 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 Fluorogenic Substrate Enterococcus Test using Enterolert Contaminant Coliphage: Method Organization Reference Title/Source Date Notes EPA 1601: Male-specific (F+) and Somatic Coliphage in Water by 1601 EPA Two-step Enrichment 2001 Procedure, April 2001 EPA Method 1601 (2001) EPA 1602: Male-specific (F+) and Somatic Coliphage in Water by 1602 EPA Single Agar Layer (SAL) Procedure, April 2001 EPA Method 1602 (2001) 2001 Fast Phage Test Presence/Absence for Coliphage in Ground Water with Same Day Fast Phage® Charm Sciences Positive Prediction, ATP Case No. D09-0007, Version 009, November 28, 2012 Fast Phage Test, v. 009 2012 ------- |