&EPA
Sr^'^on Analytical Methods Approved for Compliance Monitoring under the
Surface Water Treatment Coliform Rule
Analysis for the following contaminants shall be conducted in accordance with the methods in the following table, or their equivalent as
determined by EPA. The methods and monitoring requirements for these contaminants are specified in 40 CFR 141.74(a)(1). Additional
methods are listed in Appendix A to Subpart C of Part 141.
The CFR is the legal reference for approved methods and takes precedent over this table. The table should accurately reflect the
analytical methods information published in 40 CFR 141.
§141.74(a)(1)- Analytical and monitoring requirements
(a)(1) Analytical requirements
(1) The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10 °C during transit.
The procedures must be done in accordance with the documents listed at §141.74(a)(1) or one of the alternative methods listed in Appendix A to Subpart C of
Part 141. For Standard Methods Online, the year in which each method was approved by the Standard Methods Committee is designated by the last two digits
following the hyphen in the method number. The methods listed are the only online versions of the method that maybe used. For vendor methods, the date of
the method listed in §141.74(a)(1) or Appendix A to Subpart C of Part 141 is the date/version of the approved method. The methods listed are the only versions
that may be used for compliance with this rule. Laboratories should be careful to use only the approved versions of the methods as product packaging inserts
may not be the same as the approved versions of the methods.
Contaminant
Total Coliforms - lactose fermentation methods:
Method
Organization
Reference Title/Source
Date
Notes
9221 A, B, C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 18th Edition
Standard Methods
1992
Lactose broth, as commercially available, may be used in lieu of
lauryl tryptose broth, if the system conducts at least 25 parallel
tests between lactose broth and lauryl tryptose broth using the
water normally tested, and if the findings from this comparison
demonstrate that the false-positive rate and the false-negative
rate for total coliforms, using lactose broth, is less than 10
percent.
Media should cover inverted tubes at least one-half to two-
thirds after the sample is added.
Office of Water (MS - 4606 M)
EPA 815-B-19-008
April 2019

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Page 2 of 12
Method
Organization
Reference Title/Source
Date
Notes




No requirement exists to run the completed phase on 10
percent of all total coliform-positive confirmed tubes.
9221 A, B, C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 19th Edition
Standard Methods
1995
Lactose broth, as commercially available, may be used in lieu of
lauryl tryptose broth, if the system conducts at least 25 parallel
tests between lactose broth and lauryl tryptose broth using the
water normally tested, and if the findings from this comparison
demonstrate that the false-positive rate and the false-negative
rate for total coliforms, using lactose broth, is less than 10
percent.
Media should cover inverted tubes at least one-half to two-
thirds after the sample is added.
No requirement exists to run the completed phase on 10
percent of all total coliform-positive confirmed tubes.
9221 A, B, C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
Standard Methods
1998
Lactose broth, as commercially available, may be used in lieu of
lauryl tryptose broth, if the system conducts at least 25 parallel
tests between lactose broth and lauryl tryptose broth using the
water normally tested, and if the findings from this comparison
demonstrate that the false-positive rate and the false-negative
rate for total coliforms, using lactose broth, is less than 10
percent.
Media should cover inverted tubes at least one-half to two-
thirds after the sample is added.
No requirement exists to run the completed phase on 10
percent of all total coliform-positive confirmed tubes.
9221 A, B, C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 21st Edition
Standard Methods
2005
Lactose broth, as commercially available, may be used in lieu of
lauryl tryptose broth, if the system conducts at least 25 parallel
tests between lactose broth and lauryl tryptose broth using the
water normally tested, and if the findings from this comparison
demonstrate that the false-positive rate and the false-negative
rate for total coliforms, using lactose broth, is less than 10
percent.
Media should cover inverted tubes at least one-half to two-
thirds after the sample is added.
No requirement exists to run the completed phase on 10
percent of all total coliform-positive confirmed tubes.
9221 A, B, C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 22nd Edition
Standard Methods
2012
Lactose broth, as commercially available, may be used in lieu of
lauryl tryptose broth, if the system conducts at least 25 parallel
tests between lactose broth and lauryl tryptose broth using the
water normally tested, and if the findings from this comparison
demonstrate that the false-positive rate and the false-negative

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Page 3 of 12
Method
Organization
Reference Title/Source
Date
Notes




rate for total coliforms, using lactose broth, is less than 10
percent.
Media should cover inverted tubes at least one-half to two-
thirds after the sample is added.
No requirement exists to run the completed phase on 10
percent of all total coliform-positive confirmed tubes.
9221 A, B, C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 23rd Edition
Standard Methods
2017
Lactose broth, as commercially available, may be used in lieu of
lauryl tryptose broth, if the system conducts at least 25 parallel
tests between lactose broth and lauryl tryptose broth using the
water normally tested, and if the findings from this comparison
demonstrate that the false-positive rate and the false-negative
rate for total coliforms, using lactose broth, is less than 10
percent.
Media should cover inverted tubes at least one-half to two-
thirds after the sample is added.
No requirement exists to run the completed phase on 10
percent of all total coliform-positive confirmed tubes.
9221 A, B, C-99
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
Standard Methods
1999
Lactose broth, as commercially available, may be used in lieu of
lauryl tryptose broth, if the system conducts at least 25 parallel
tests between lactose broth and lauryl tryptose broth using the
water normally tested, and if the findings from this comparison
demonstrate that the false-positive rate and the false-negative
rate for total coliforms, using lactose broth, is less than 10
percent.
Media should cover inverted tubes at least one-half to two-
thirds after the sample is added.
No requirement exists to run the completed phase on 10
percent of all total coliform-positive confirmed tubes.
9221 A, B, C-06
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
Standard Methods
2006
Lactose broth, as commercially available, may be used in lieu of
lauryl tryptose broth, if the system conducts at least 25 parallel
tests between lactose broth and lauryl tryptose broth using the
water normally tested, and if the findings from this comparison
demonstrate that the false-positive rate and the false-negative
rate for total coliforms, using lactose broth, is less than 10
percent.
Media should cover inverted tubes at least one-half to two-
thirds after the sample is added.
No requirement exists to run the completed phase on 10
percent of all total coliform-positive confirmed tubes.

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Page 4 of 12
Total coliforms - membrane filtration methods:
Method
Organization
Reference Title/Source
Date
Notes
9222 A, B, C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 18th Edition
Standard Methods
1992

9222 A, B, C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 19th Edition
Standard Methods
1995

9222 A, B, C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
Standard Methods
1998

9222 A, B, C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 21st Edition
Standard Methods
2005

9222 A, B, C
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 23rd Edition
Standard Methods
2017

9222 A, B, C-97
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
Standard Methods
1997

1604
EPA
Method 1604: Total Coliforms
and Escherichia coli in Water by
Membrane Filtration Using a
Simultaneous Detection
Technique (Ml Medium),
September 2002
EPA Method 1604
2002

Total coliforms - enzyme substrate methods:

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Page 5 of 12
Method
Organization
Reference Title/Source
Date
Notes
9223
Colilert
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 18th Edition
Standard Methods
1992

9223
Colilert
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 19th Edition
Standard Methods
1995

9223
Colilert
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
Standard Methods
1998

9223
Colilert
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 21st Edition
Standard Methods
2005

9223 B
Colilert
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 22nd Edition
Standard Methods
2012

9223 B
Colilert
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 23rd Edition
Standard Methods
2017

9223 B-97
Colilert
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
1997

9223 B-04
Colilert
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
2004

Contaminant
Fecal coliforms - Fecal Coliform Procedure (following Lactose Fermentation Methods):

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Page 6 of 12
Method
Organization
Reference Title/Source
Date
Notes
9221 E
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 18th Edition
Standard Methods
1992
A-l broth may be held up to 7 days in a tightly closed screw cap
tube at 4°C
9221 E
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 19th Edition
Standard Methods
1995
A-l broth may be held up to 7 days in a tightly closed screw cap
tube at 4°C
9221 E
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
Standard Methods
1998
A-l broth may be held up to 7 days in a tightly closed screw cap
tube at 4°C
9221 E
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 21st Edition
Standard Methods
2005
A-l broth may be held up to 7 days in a tightly closed screw cap
tube at 4°C
9221 E
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 22nd Edition
Standard Methods
2012
A-l broth may be held up to 7 days in a tightly closed screw cap
tube at 4°C
9221 E
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 23rd Edition
Standard Methods
2017
A-l broth may be held up to 7 days in a tightly closed screw cap
tube at 4°C
9221 E-99
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
Standard Methods
1999
A-l broth may be held up to 7 days in a tightly closed screw cap
tube at 4°C
9221 E-06
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
Standard Methods
2006
A-l broth may be held up to 7 days in a tightly closed screw cap
tube at 4°C

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Page 7 of 12
Contaminant
Fecal coliforms - membrane filtration methods:
Method
Organization
Reference Title/Source
Date
Notes
9222 D
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 18th Edition
Standard Methods
1992

9222D
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 19th Edition
Standard Methods
1995

9222 D
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
Standard Methods
1998

9222D
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 21st Edition
Standard Methods
2005

9222 D
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 22nd Edition
Standard Methods
2012

9222 D
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 23rd Edition
Standard Methods
2017

9222 D-97
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
Standard Methods
1997


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Page 8 of 12
Method
Organization
Reference Title/Source
Date
Notes
9222 D-06
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
Standard Methods
2006

Contaminant
Heterotrophic bacteria - culture method:
Method
Organization
Reference Title/Source
Date
Notes
9215 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 18th Edition
Standard Methods
1992

9215 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 19th Edition
Standard Methods
1995

9215 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
Standard Methods
1998

9215 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 21st Edition
Standard Methods
2005

9215 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 22nd Edition
Standard Methods
2012

9215 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 23rd Edition
Standard Methods
2017


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Page 9 of 12
Method
Organization
Reference Title/Source
Date
Notes
9215 B-00
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
Standard Methods
2000

9215 B-04
Standard Methods Online
Online version. Approval year is
designated by the last 2 digits.
Only online versions cited in the
regulations or in Appendix A to
Subpart C of Part 141 are
approved.
Standard Methods
2004

Contaminant
Heterotrophic bacteria - enzyme substrate methods:
Method
Organization
Reference Title/Source
Date
Notes
Simplate
IDEXX
IDEXX SimPlate™ HPCtest
method for Heterotrophs in
Water, November 2000.
IDEXX Laboratories, One IDEXX
Drive, Westbrook, ME 04092
2000

Contaminant
Turbidity methods:
Method
Organization
Reference Title/Source
Date
Notes
2130 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 18th Edition
Standard Methods
1992
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
2130 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 19th Edition
Standard Methods
1995
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin

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Page 10 of 12
Method
Organization
Reference Title/Source
Date
Notes
2130 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 20th Edition
Standard Methods
1998
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
2130 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 21st Edition
Standard Methods
2005
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
2130 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 22nd Edition
Standard Methods
2012
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
2130 B
Standard Methods
Standard Methods for the
Examination of Water and
Wastewater, 23rd Edition
Standard Methods
2017
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
180.1
EPA
Methods for the Determination
of Inorganic Substances in
Environmental Samples,
EPA/600/R-93/100,
August 1993
National Environmental
Methods Index
1993
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
Method 2
Great Lakes Instruments
Great Lakes Instruments
Method 2, Turbidity,
November 2,1992
GLI Method 2
1992
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
10133
Hach
Hach FilterTrak Method 10133,
Revision 2.0,
Determination of Turbidity by
Laser Nephelometry,
January 2000
Hach FilterTrak Method 10133
2000
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
M5271
Leek Mitchell
Mitchell Method M5271,
Revision 1.1,
Determination of Turbidity by
Laser Nephelometry,
March 5, 2009
Mitchell M5271, Rev. 1.1
2009
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin

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Page 11 of 12
Method
Organization
Reference Title/Source
Date
Notes
M5331
Leek Mitchell
Mitchell Method M5331,
Revision 1.1,
Determination of Turbidity by
LED Nephelometry,
March 5, 2009
Mitchell M5331, Rev. 1.1
2009
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
AMI Turbiwell
Swan Analytische Instrumente AG
Continuous Measurement of
Turbidity Using a SWAN AMI
Turbiwell Turbidimeter,
August 2009
SWAN AMI Turbiwell
2009
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
AQ4500
Thermo Scientific
Orion Method AQ4500, Revision
1.0, Determination of Turbidity
by LED Nephelometry,
May 8, 2009
Orion AQ4500, Rev. 1.0
2009
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
M5331
(Rev. 1.2)
Leek Mitchell
Mitchell Method M5331,
Revision 1.2
Determination of Turbidity by
LED or Laser Nephelometry,
February 2016
Mitchell M5331, Rev. 1.2
2016
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
10258
(Rev. 1.0)
Hach Company
Hach Method 10258, Rev. 1.0
Determination of Turbidity by
360° Nephelometry,
January 2016
Hach 10258, Rev. 1.0
2016
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
10258
(Rev. 2.0)
Hach Company
Hach Method 10258, Rev. 2.0
Determination of Turbidity by
360° Nephelometry,
March 2018
2018
Revised method allows the use of sealed vials prefilled with
Hach StablCal™ for quarterly calibration
8195
(Rev. 3.0)
Hach Company
Hach Method 8195, Rev. 3.0
Determination of Turbidity by
Nephelometry,
March 2018
2018
Revised method allows the use of sealed vials prefilled with
Hach StablCal™ for quarterly calibration

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Page 12 of 12
Method
Organization
Reference Title/Source
Date
Notes
Lovibond PTV
1000
Tintometer, Inc.
Continuous Measurement of
Drinking Water Turbidity using a
Lovibond PTV 1000 White Light
LED Turbidimeter,
December 2016
Lovibond PTV 1000, Rev. 1.0
2016
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
Lovibond PTV
2000
Tintometer, Inc.
Continuous Measurement of
Drinking Water Turbidity using a
Lovibond PTV 2000 660-nm LED
Turbidimeter,
December 2016
Lovibond PTV 2000, Rev. 1.0
2016
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin
Lovibond PTV
6000
Tintometer, Inc.
Continuous Measurement of
Drinking Water Turbidity using a
Lovibond PTV 6000 Laser
Turbidimeter,
December 2016
Lovibond PTV 6000, Rev. 1.0
2016
Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or
equivalent) and stablilized formazin (e.g., Hach StablCal™ or
equivalent) are acceptable substitutes for formazin

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