EPA/600/R-17/389 | September 2017 www.epq.qov/hoinelarid-securitv-research United States Environmental Protection Agency Sample Collection Information Document for Chemicals, Radiochemicals and Biotoxins Companion to Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2017 Office of Research and Development Homeland Security Research Program ------- This page left intentionally blank ------- Sample Collection Information Document for Chemicals, Radiochemicals and Biotoxins Companion to Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2017 by Romy Campisano, Kathy Hall, Stuart Willison Threat and Consequence Division Matthew Magnuson Water Infrastructure Protection Division National Homeland Security Research Center Cincinnati, OH 45268 John Griggs National Analytical Radiation Environmental Laboratory Office of Air and Radiation Office of Radiation and Indoor Air Montgomery, AL 36115 Steve Reimer U.S. EPA Region 10 - Manchester Laboratory Port Orchard, WA 98366 Heath Mash National Risk Management Research Laboratory Cincinnati, OH 45268 ------- SAM Companion - Sample Collection Information Document Disclaimer This document is undergoing review in accordance with EPA policy prior to publication. Note that approval for publication does not signify that the contents necessarily reflect the views of the Agency. Mention of trade names, products, or services does not convey EPA approval, endorsement, or recommendation. Questions concerning this document or its application should be addressed to: Romy Campisano National Homeland Security Research Center Office of Research and Development (NG16) U.S. Environmental Protection Agency 26 West Martin Luther King Drive Cincinnati, OH 45268 (513) 569-7016 campisano.romv@, epa.gov Kathy Hall National Homeland Security Research Center Office of Research and Development (NG16) U.S. Environmental Protection Agency 26 West Martin Luther King Drive Cincinnati, OH 45268 (513) 379-5260 hall.kathv@,epa.gov ii September 2017 ------- SAM Companion - Sample Collection Information Document Acronyms and Abbreviations AOAC AOAC International (formerly the Association of Official Analytical Chemists) Anal. Chem. Analytical Chemistry APHA American Public Health Association ASTM ASTM International (formerly American Society for Testing and Materials) BZ Quinuclidinyl benzilate °C Degree Celsius CDC U.S. Centers for Disease Control and Prevention CFR Code of Federal Regulations CLP Contract Laboratory Program cm Centimeter(s) cm2 Centimeter(s) squared COC Chain of custody CVAA 2-Chlorovinylarsonous acid CVAOA 2-Chlorovinylarsonic acid DAS Diacetoxyscirpenol DGR Dangerous Goods Regulations DHS U.S. Department of Homeland Security DIMP Diisopropyl methylphosphonate DNPH Dinitrophenylhydrazine DOE U.S. Department of Energy DOL U.S. Department of Labor DOT U.S. Department of Transportation EA2192 [S-2-(diisopropylamino) ethylmethylphosphono-thioic acid] ECL Electrochemiluminescence ED Ethyldichloroarsine EDEA N-Ethyldiethanolamine ELFA Enzyme-linked fluorescent immunoassay ELISA Enzyme-linked immunosorbent assay EMPA Ethyl methylphosphonic acid EMSL Environmental Monitoring and Support Laboratory EPA U.S. Environmental Protection Agency ERT Environmental Response Team (EPA) ft2 Square foot (square feet) FDA U.S. Food and Drug Administration FRET Fluorescence resonance energy transfer FRMAC Federal Radiological Monitoring and Assessment Center g Gram(s) GA Tabun gal Gallon GB Sarin GD Soman GE 1-Methylethyl ester ethyl phosphonofluoridic acid GF Cyclohexyl sarin GPS Global Positioning System HASL Health and Safety Laboratory, currently known as Environmental Measurements Laboratory (EML) HASP Health and Safety Plan HC1 Hydrochloric acid HD Mustard, sulfur Hg Mercury Hg(CN)2 Mercury (II) cyanide iii September 2017 ------- SAM Companion - Sample Collection Information Document HILIC MS-MS 2-HMP HMTD HMX HN-1 HN-2 HN-3 HN03 HPLC-FL HSRP i.d. I AT A IMPA 10 ISM ISO kPpa L L-l L-2 L-3 lbs LC-ESI-MS-MS LC-MS LC-MS-MS LC-UV LFA M m3 jiL (im MALDI-TOF-MS MCE MDEA mg min mL mm mM MPA NA Na2C03 NaOH NAREL NEMI NH3 NH4CI NH4OH NHSRC NIOSH n.o.s. NTT A A o.d. Hydrophilic interaction-tandem mass spectrometry (2-[hydroxymethyl] piperidine) Hexamethylenetriperoxidediamine Octahydro-1,3,5,7-tetranitro-1,3,5,7 -tetrazocine Mustard 1, nitrogen Mustard 2, nitrogen Mustard 3, nitrogen Nitric acid High performance liquid chromatography-fluorescence Homeland Security Research Program Inner diameter International Air Transportation Association Isopropyl methylphosphonic acid Inorganic Inorganic Superfund Methods International Organization for Standardization Kilopascals Liter(s) Lewisite 1 Lewisite 2 Lewisite 3 Pounds Liquid chromatography-electrospray ionization-tandem mass spectrometry Liquid chromatography-mass spectrometry Liquid chromatography-tandem mass spectrometry Liquid chromatography-ultraviolet Lateral flow immunoassay Molar Cubic meter(s) Microliter(s) Micrometer(s) Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry Mixed cellulose ester N-Methyl diethanolamine Milligram(s) Minute(s) Milliliter(s) Millimeter(s) Millimolar Methylphosphonic acid Not applicable Sodium carbonate Sodium hydroxide National Air and Radiation Environmental Laboratory (EPA) National Environmental Methods Index Ammonia Ammonium chloride Ammonium hydroxide National Homeland Security Research Center (EPA) National Institute for Occupational Safety and Health Not otherwise specified National Technology Transfer and Advancement Act Outer diameter IV September 2017 ------- SAM Companion - Sample Collection Information Document OMB Office of Management and Budget Directives ORD Office of Research and Development (EPA) ORISE Oak Ridge Institute for Science and Education OSHA Occupational Safety and Health Administration OVS OSHA versatile sampler OW Office of Water (EPA) oz. Ounce (s) PETN Pentaerythritol tetranitrate Pg- Page(s) PHILIS Portable High-Throughput Integrated Laboratory Identification Systems PMPA Pinacolyl methyl phosphonic acid ppbv Parts-per-billion by volume PPE Personal protective equipment PPm Parts per million pptv Parts per trillion by volume psig Pounds per square inch gauge PSN Proper shipping name PTFE Polytetrafluoroethylene PUF Polyurethane foam PVC Polyvinyl chloride QC Quality control R 33 Methylphosphonic acid, S-[2-(diethylamino)ethyl]0-2-methylpropylester] RDX Hexahydro-1,3,5 -trinitro-1,3,5 -triazine RESL Radiological and Environmental Sciences Laboratory Rev Revision SAM Selected Analytical Methods for Environmental Remediation and Recovery SCID Sample Collection Information Document SCP Sample Collection Plan SM Standard Methods for the Examination of Water and Wastewater SOP Standard Operating Procedure SRS Savannah River National Laboratory, Savannah River Site SW Solid Waste TBD To be determined TDG Thiodiglycol TEA T riethanolamine TEPP Tetraethyl pyrophosphate TETS T etramethylenedisulfotetramine TI Transportation Index 1,3,5-TNB 1,3,5 -T rinitrobenzene 2,4,6-TNT 2,4,6-Trinitrotoluene TO Toxic organics UN# United Nations numbers USGS United States Geological Survey VCSB Voluntary Consensus Standards Body VOA Volatile Organic Analysis Vol. Volume v September 2017 ------- SAM Companion - Sample Collection Information Document Acknowledgments This document was developed by the U.S. Environmental Protection Agency's (EPA) Homeland Security Research Program (HSRP) within EPA's Office of Research and Development as a companion to Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2017. The document was prepared by CSRA Inc. under EPA Contract No. EP-C-15-012. Contributions of the following individuals and organizations to the development of the information provided in this document are acknowledged: • Joan Bursey, EPA, NHSRC (SEE Grantee) • Romy Campisano, EPA, NHSRC • Lesley V. D'Anglada, EPA, Office of Water • George Gardenier, EPA, Office ofWater • Diane Gregg, EPA, Region 6 Laboratory • John Griggs, EPA, National Analytical Radiation Environmental Laboratory • Kathy Hall, EPA, NHSRC • Scott Hudson, EPA, Office of Land and Emergency Management • Mario Ierardi, EPA, Office of Land and Emergency Management • Matthew Magnuson, EPA, NHSRC • Heath Mash, EPA, National Risk Management Research Laboratory • Emily Parry, EPA, NHSRC • Steve Reimer, EPA, Region 10 Laboratory • Sanjiv Shah, EPA, NHSRC • Erin Silvestri, EPA, NHSRC • James Sinclair, EPA, Office ofWater • Gerard Stelma, EPA, National Exposure Research Laboratory • Allison Tamasi, EPA, National Exposure Research Laboratory • Sue Warner, EPA, Region 3 Laboratory • Ernest Waterman, EPA, New England Regional Laboratory • Stuart Willison, EPA, NHSRC • Jeanette Van Emon, EPA, National Exposure Research Laboratory • Shen-yi Yang, EPA, Office of Land and Emergency Management • Rebecca Coleman, U.S. Centers for Disease Control and Prevention • Marcie Jaffee, U.S. Centers for Disease Control and Prevention • Richard Wang, U.S. Centers for Disease Control and Prevention • Luisa Cheng, U.S. Department of Agriculture • Xiaohua He, U.S. Department of Agriculture • Gretchen Farming, U.S. Air Force • Marty Johnson, U.S. Army • Aurelie Soreefan, U.S. Air Force • Sara McGrath, U.S. Food and Drug Administration • Shashi Sharma, U.S. Food and Drug Administration • Peter Moeller, National Oceanic & Atmospheric Administration • Donivan Porterfield, Los Alamos National Laboratory vi September 2017 ------- SAM Companion - Sample Collection Information Document • Sanwat Chaudhuri, Utah Public Health Laboratory • Heather Raymond, Ohio EPA • Anthony Rattonetti, San Francisco Public Utilities Commission Southeast Laboratory • Bob Read, Tennessee Department of Health • Christopher Retarides, Virginia Division of Consolidated Laboratory Services • Eric Boring, CSRA, Inc. • John Chandler, CSRA, Inc. • Sang Chung, Consolidated Safety Services, Inc. • Joan Cuddeback, CSRA, Inc. • W. Gordeuk, Consolidated Safety Services, Inc. • Kelly Head, Consolidated Safety Services, Inc. • Emily King, CSRA, Inc. • Robert Rosson, CSRA, Inc. • Sarah Staggs, Consolidated Safety Services, Inc. • Jim Travis, Consolidated Safety Services, Inc. vii September 2017 ------- SAM Companion - Sample Collection Information Document This page left intentionally blank viii September 2017 ------- SAM Companion - Sample Collection Information Document Table of Contents DISCLAIMER M ACRONYMS AND ABBREVIATIONS Mi ACKNOWLEDGMENTS vi LIST OF ATTACHMENTS ix SAMPLE COLLECTION INFORMATION DOCUMENT 1 1.0 BACKGROUND 1 2.0 SCOPE AND APPLICATION 1 2.1 Sample Collection Information Attachments 2 2.2 Document Development 2 2.3 Limitations 4 3.0 HEALTH AND SAFETY CONSIDERATIONS 4 3.1 Heal th and Safety Plans 4 3.2 Personal Protective Equipment and Monitoring 5 3.3 Training 5 4.0 PREPARATION FOR SAMPLE COLLECTION 5 4.1 Field Sampling Equipment and Supplies 6 4.2 Field Data Documentation 6 4.3 Equipment Operation and Calibration 7 5.0 PREPARATION OF SAMPLE CONTAINERS 7 5.1 Sample Container Labels 7 5.2 Preparing Sample Containers for Packaging 7 5.3 General Sample Shipment Guidelines 7 5.4 Chain-of-Custody Forms 8 5.5 Custody Seals/Tamper-evidentBags 8 6.0 DEFINITIONS 9 7.0 BIBLIOGRAPHY 11 List of Attachments Attachment A: Sample Collection Information for the Chemical Analytes and Methods Listed in SAM 2017 Attachment B1: Sample Collection Information for the Environmental Sample Types, Radiochemical Analytes and Methods Listed in SAM 2017 Attachment B2: Sample Collection Information for the Outdoor Building and Infrastructure Materials, Radiochemical Analytes and Methods Listed in SAM 2017 Attachment C: Sample Collection Information for the Biotoxins and Methods Listed in SAM 2017 ix September 2017 ------- This page left intentionally blank ------- SAM Companion - Sample Collection Information Document Sample Collection Information Document for Chemicals, Radiochemicals and Biotoxins [Companion to Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2017] 1.0 Background The U.S. Environmental Protection Agency's (EPA's) Homeland Security Research Program (HSRP) has worked with experts from across EPA and its sister agencies since 2003 to develop a compendium of analytical methods to be used when responding to intentional or unintentional contamination incidents. Analytical methods have been selected for chemical, radiochemical, pathogen and biotoxin analytes of concern for the types of environmental sample matrices that are anticipated in such incidents. The results of these efforts have been published in several revisions of EPA's Selected Analytical Methods for Environmental Remediation and Recovery (SAM).1 HSRP periodically reviews and updates this information to address the needs of homeland security, reflect improvements in analytical methods and new technologies, and incorporate changes in target analytes. In support of efforts to select appropriate analytical methods, EPA also recognizes the need for companion documents to provide information regarding collection of samples for analysis using the selected methods. This document is intended to address this need, in part, by providing information regarding sample containers, preservation, size, packaging and sources for additional information supporting collection of chemical, radiochemical and biotoxin samples to be analyzed using the methods selected. As with SAM 2017, HSRP plans to update the information in this document periodically, to reflect changes to the analytes addressed and/or methods that have been selected. The information contained in this document is intended to support and be used with the methods listed in EPA's SAM 2017. The information will be reviewed and updated periodically, along with the selected methods, to reflect advances in technologies, results of method evaluation and validation studies, and additional analytes or matrices. 2.0 Scope and Application This Sample Collection Information Document (SCID) provides general information for use by EPA and its contractors when collecting samples during environmental remediation following a contamination incident. The document is intended to be used with SAM 2017 (EPA/600/R- 17/356; U.S. EPA 2017) and to provide information needed for collection of samples to be analyzed using the specific methods and procedures that have been selected for chemicals, radiochemicals and biotoxins. Where possible, the information provided was obtained from the sample collection requirements and guidelines included in the analytical methods. Where this information was not available, additional sources were used (see Section 7.0). The information in this document is intended to be used during site assessment, remediation and clearance activities following a contamination incident; it assumes that samples will be collected 1 Selected Analytical Methods for Environmental Remediation and Recovery (SAM) and its methods are available at: https ://www. epa. gov/homeland-securitv-research/sam. 1 September 2017 ------- SAM Companion - Sample Collection Information Document by personnel trained in collection of environmental samples suspected of containing the target analytes and in dealing with the corresponding safety concerns. Information is included regarding containers, collection volume or weight, preservation, holding times, and packaging of samples representing the sample types and analytes included in SAM 2017. Sample collection information for pathogens is addressed in a separate document.2 Sample collection personnel should consult the incident-specific Sample Collection Plan (SCP) and/or receiving laboratory(ies) prior to initiating sample collection activities. NOTE: It is possible that some of the information in this document should be modified to address site- or incident-specific data needs; for example, additional sample volume may be needed for quality control (QC) or in cases where an unusually low level is required to meet site clearance goals. Sample Collection Plans (SCPs) should be in place and consulted for specific sample collection requirements prior to initiation of sample collection activities. Site- or incident-specific SCPs include information regarding laboratory capacity; the extent of contamination; target analytes, including their preservation and holding times; data quality objectives; sample locations; and the number and type of samples needed. 2.1 Sample Collection Information Attachments This document contains the following attachments listing information for collection of samples that will be analyzed for measurement of the analytes listed in Selected Analytical Methods for Environmental Remediation and Recovery. • Attachment A: Sample Collection Information for the Chemical Analytes and Methods Listed in SAM 2017 • Attachment B1: Sample Collection Information for the Environmental Samples, Radiochemical Analytes and Methods Listed in SAM 2017 • Attachment B2: Sample Collection Information for the Outdoor Building and Infrastructure Materials, Radiochemical Analytes and Methods Listed in SAM 2017 • Attachment C: Sample Collection Information for the Biotoxins and Methods Listed in SAM 2017 Each attachment provides the sample size that should be collected to support sample analysis, the preservatives and/or temperature needed to maintain sample integrity prior to analysis, the maximum amount of time that should elapse between sample collection and the initiation of analytical procedures (e.g., sample analysis, digestion or extraction), the appropriate type of container, the sample label and packaging procedures needed for sample shipment, and the source(s) used to provide the information. The information in each attachment is arranged by analyte and corresponds to the sample types that are addressed by the analytical methods that have been selected. 2.2 Document Development EPA employed several references to identify the information that is included in this 2 EPA's Sample Collection Information Document for Pathogens - Companion to SAM 2017 (U.S. EPA. EPA/600/R-17/374) is available at: https://www.epa.gov/homeland-securitv-research/sam-companion-documents- and-sample-collection-procedures 2 September 2017 ------- SAM Companion - Sample Collection Information Document document. The first sources consulted were the methods listed in SAM 2017. These methods are listed in the "Selected Analytical Methods" columns throughout Appendices A, Bl, B2 and C. If the selected methods include sample collection information, the information was evaluated and, if appropriate, included in the sample collection information tables provided in Attachments A, Bl, B2 and C. The second sources consulted were EPA procedures for collection of samples addressing the specific analyte/matrix pairs. If there were no EPA procedures available, other federal agency or Voluntary Consensus Standards Body (VCSB)3 methods were consulted, and best professional judgment applied by technical work groups to address collection of each of the analyte/sample type pairs. In many cases, information was adjusted for consistency across similar analytes (e.g., volatile organic compounds) and sample types, to facilitate sample collection without compromising analytical results. Information was evaluated from the following agencies, organizations, and publications: • EPA - United States Environmental Protection Agency • AO AC International (formerly Association of Official Analytical Chemists) • ASTM - ASTM International (formerly American Society for Testing and Materials) • CFR - Code of Federal Regulations • U.S. CDC - United States Centers for Disease Control and Prevention • USDA - United States Department of Agriculture • U.S. DHS - United States Department of Homeland Security • U.S. DOE - United States Department of Energy • U.S. DOL - United States Department of Labor • U.S. DOT - United States Department of Transportation • U.S. FDA - United States Food and Drug Administration • USGS - United States Geological Survey • IATA - International Air Transport Association • ISO - International Organization for Standardization • NEMI - National Environmental Methods Index • NIOSH - National Institute for Occupational Safety and Health • OPCW - Organisation for the Prohibition of Chemical Weapons • OSHA - Occupational Safety and Health Administration • Standard Methods for the Examination of Water and Wastewater, 22nd Edition. 2005. American Public Health Association • Journals: Analyst, Chromatographia, European Journal of Lipid Science and Technology, Journal of Chromatography A, Journal of Chromatography B, Journal of Forensic Sciences, Water Research Sample collection information was not identified for all the analyte/sample type pairs listed in SAM 2017. In some cases, "TBD" (to be determined) is used to identify gaps of information that have yet to be determined. In other cases, analytes are listed as not a concern in a particular sample type; in these cases, SAM work groups have determined that the analyte is not a concern due to a number of factors, including low likelihood of persistence, toxicity, mobility or solubility. 3 EPA OMB Circular No. A-l 19, February 1998. VCSBs are domestic or international organizations which plan, develop, establish, or coordinate voluntary consensus standards using agreed-upon procedures. Under the National Technology Transfer and Advancement Act (NTTAA), participation of federal representatives in VCSBs is encouraged to increase the likelihood that the standards they develop will meet both public and private sector needs. 3 September 2017 ------- SAM Companion - Sample Collection Information Document 2.3 Limitations This document provides summary information only regarding collection of samples to be analyzed for measurement of the chemicals, radiochemicals and biotoxins listed in SAM 2017. It does not provide details regarding sample collection procedures or the corresponding analytical methods. Although at this time much of the information has not been tested for some of the analytes or sample types, the information listed is considered to be the most appropriate currently available. Many of the target analytes listed in this document have only recently become an environmental concern, and EPA is actively pursuing development and validation of appropriate sample collection procedures. Users should consult the SAM website for periodic updates: https://www.epa.gov/homeland- securitv-research/sam. Sample collection plans must be consulted for site- or incident-specific requirements, including QC and reporting. The information sources cited in this document also should be consulted for additional details regarding sample collection, including QC requirements, sample handling, packaging, shipping, and safety procedures. Sample collectors should check with the incident commanders for special instructions regarding evidentiary matters prior to sample collection. 3.0 Health and Safety Considerations This document assumes that a site- or incident-specific Health and Safety Plan (HASP) is in place that includes the safety concerns and requirements regarding the specific types of hazards that should be considered during sample collection. This section provides general guidelines regarding health and safety concerns. At a minimum, all sampling team members should be trained in Occupational Safety and Health Administration (OSHA) requirements for hazardous waste operations and emergency response at 29 CFR 1910.120 or 29 CFR 1926.65 and have current medical screening. 3.1 Health and Safety Plans Health and Safety Plans (HASPs) will vary depending on the site, the sampling phase (site assessment, remediation or final status determination) and the responsible organization. The purpose of these plans is to ensure maximum protection to workers, the environment and surrounding communities, in a way that is consistent with requirements needed to perform operational activities. HASPs should follow guidelines provided by OSHA at: https://www.osha.gov/Publications/OSHA3114/OSHA-3114-hazwoper.pdf (last accessed September 2017). At a minimum, HASPs should include instructions and guidelines regarding: Names, positions, and contact information of key personnel and of health and safety personnel Site- or incident-specific risk assessment addressing sample collection activities Training requirements Personal protective equipment (PPE) on site and usage requirements Medical screening requirements (maintain confidential documents properly and securely) Site or incident control Emergency response plan, containing off-site emergency contact information such as local hazardous materials response teams or additional trained rescue personnel (29 CFR 1910.38) 4 September 2017 ------- SAM Companion - Sample Collection Information Document Entry and egress procedures Spill containment Decontamination procedures NOTE: Entry and decontamination procedures should address personnel monitoring and decontamination during entry and egress. 3.2 Personal Protective Equipment and Monitoring Each site or incident also will dictate the level of PPE that will be required. Selection of protective clothing is dependent on site conditions and sample collection requirements included in the SCP. Specific guidance for selection of PPE is provided in 29 CFR 1910.120, Appendix B. Factors that should be considered during selection include: contaminant identification, routes of exposure (i.e., inhalation, skin absorption, ingestion and injection), performance of equipment in protecting against exposure, activity duration, and stress induced by work requirements. Because the use of PPE can also cause hazards to workers (e.g., heat stress, impaired vision and mobility), care should be taken to provide a level of protection that is sufficient to prevent exposure yet is not so high as to create other unnecessary hazards. 3.3 Training Sample collectors must be trained in collection and handling of samples suspected of containing the contaminants of concern (see Attachments A, Bl, B2 and C) and must be up to date regarding medical screening requirements. Additionally, sample collectors and packers must be trained in and comply with the site HASP and all site-specific training requirements, including the following: Ability to select and work with the appropriate level of PPE Ability to utilize personnel monitoring equipment Decontamination procedures Prevention of sample cross-contamination Training frequency and documentation 4.0 Preparation for Sample Collection It is highly recommended that sampling kits be used during sample collection, and that these kits be properly equipped, maintained, and organized before deployment of sample collection personnel. These kits should be checked for quality assurance prior to initiation of sample collection activities. Sample collectors should consult with project managers and the SCP to determine what equipment and materials should be assembled, whether samples should be preserved in the field, and how sample containers will be packaged for shipment. Prior to sample collection, sample collectors should also contact the laboratory that will be analyzing the samples to understand laboratory-specific requirements. Sample kits should contain all containers, materials, supplies and forms needed to perform sample collection, decontamination, documentation and field packaging activities. Procedures for preparing samples for shipment should be in place and understood. 5 September 2017 ------- SAM Companion - Sample Collection Information Document 4.1 Field Sampling Equipment and Supplies Before starting field sampling activities, all necessary equipment and supplies should be checked, identified and available. The following is a preliminary list: Sampling devices (e.g., soil coring device, water autosampler, air filter canister or collection tube, wipes) Preservatives and dechlorinating agents (if feasible, it is recommended that preservatives and dechlorinating agents be added to sample containers prior to sample collection) Sample volumetric measuring devices and/or weighing devices Sample containers and packaging materials, including ice • PPE Record keeping devices (e.g., logs, chain-of-custody [COC] forms, writing instruments) Site maps, Global Positioning System (GPS) recorders, etc. Sample location markers Shipping containers, shipping forms, and shipping labels Waste containers Materials for cleaning equipment and sample container exteriors Samples should be collected using dedicated, sterile or sufficiently clean sampling equipment to minimize interferences and cross-contamination. In some cases, pre- packaged equipment and materials are available and can be used for efficiency. When disposable equipment is not used, contaminated equipment must be appropriately cleaned and decontaminated prior to reuse. 4.2 Field Data Documentation All data collected in the field should be adequately documented. Documented information should include (for example): Names of field sampling personnel Sample Collection Plan (SCP) Sample location(s) Date and time of sampling Sample identification number Sampling depth Sample type Sample collection medium Physical and meteorological conditions Expected contaminants Expected radionuclides (if applicable) Sample size (weight, volume), sample duration (air filters), air volume, etc. Sample handling precautions Radiological dose rate measurements of samples performed in the field should be documented and transmitted to the laboratory along with the samples. Documentation should include the background count rates; units; and the type, serial number and 6 September 2017 ------- SAM Companion - Sample Collection Information Document calibration date of the instrument used to obtain the dose rate. It is EPA Policy to use Scribe4 wherever practical to collect, store and report sampling and analytical data. 4.3 Equipment Operation and Calibration Sampling equipment should be verified to be in working order before it is deployed with the sampling teams. Equipment should be operated in accordance with manufacturer's operating instructions (unless otherwise specified in the SCP). Equipment also should undergo routine maintenance according to manufacturer instructions, and be calibrated and the calibration documented prior to use. 5.0 Preparation of Sample Containers 5.1 Sample Container Labels Each sample container has a label that provides information uniquely identifying and describing the sample. A single, unique label is affixed to each individual sample container, sample information is added in waterproof ink, and the label is covered with clear tape. Alternatively, a pre-prepared label that uniquely identifies the sample, such as a bar code that tracks the sample information, can be affixed to the container. Sample container labels include information regarding the date and time of sample collection; sample collection site; date, time and type of sample preservation; sample identification numbers; and the names and signatures of sample collectors. 5.2 Preparing Sample Containers for Packaging Once samples have been collected and preserved as specified, the containers are prepared for shipment to the analytical laboratory. Sample containers should be labeled, sealed, and decontaminated and/or disinfected prior to packing into transport or shipping containers. For samples containing radionuclides, each sample container should have dose rate measurements taken and surface contamination surveys completed, and the results recorded on a field sample tracking form (see EPA's Sample Collection Procedures for Radiochemical Analvtes in Environmental Matrices. July 2012 and Sample Collection Procedures for Radiochemical Analytes in Outdoor Building and Infrastructure Materials. September 2016). Summary procedures for cleaning and sealing sample containers are provided in the tables included in Attachments A, Bl, B2 and C. General information regarding packing samples into transport containers outside the contaminated area is provided as a footnote to each table. 5.3 General Sample Shipment Guidelines Sample collectors are responsible for ensuring compliance with DOT and IATA regulations regarding the transfer of hazardous substances and environmental samples. Regulations 49 CFR 172 and 173 for DOT (organized by separate subparts as references in Section 7.0), and the Dangerous Goods Regulations (DGR) for IATA, provide specific details regarding proper marking, labeling, placarding, packaging and shipment of hazardous materials, substances and wastes. Regulatory exceptions, such as those for 4 Scribe is a software tool developed by EPA's Environmental Response Team (ERT) to assist in managing environmental data. For additional information regarding this tool see https://www.epa.gov/ert/environmental- response-team-information-management (last accessed September 2017). 7 September 2017 ------- SAM Companion - Sample Collection Information Document shipping environmental samples, are also included. These regulations must be consulted prior to preparation of or planning for sample shipment. Summary information regarding appropriate labeling and packaging of sample transport containers is provided in the sample collection information tables included in Attachments A, Bl, B2 and C. 5.4 Chain-of-Custody Forms COC forms create a written record that can be used to trace the creation, possession, and handling of the sample from the moment of its collection through analysis. A COC form accompanies each sample or group of samples as custody of the sample(s) is transferred from one custodian to another. One copy of the form is retained by the original sample collector. If multiple laboratories are receiving a sample, individual COCs are provided to each individual laboratory, each COC representing the contents of the sample shipment. Sample collectors are responsible for the initial maintenance and completion of COC forms. Although COC forms vary in style, format and detail, the forms should contain the same minimal information required to identify the sample and to document custody. EPA policy is to use Scribe (see Section 4.2) wherever practical to generate COC forms. At a minimum, sample collectors are responsible for providing the following information on the COC form: General incident information (sample owners, contact information, site name) Sample information (e.g., sample identification number, sample type [matrix], whether grab or composite, number and type of sample containers, and date/time sample was collected) Date and time the sample was relinquished Signature of persons transferring and receiving the samples 5.5 Custody Seals / Tamper-evident Bags Custody seals are attached over the cap of each sample container to ensure the sample has not been opened or tampered with after collection and packaging. A custody seal also can be placed over the shipping or transport container, making it impossible to open the container without ripping the seal. Typically, there is one seal per sample container and two seals are placed on opposite sides of the shipping container. Custody seals contain the signature of the person responsible for packing the container and the date sealed. The seal must be sturdy to resist incidental contact but able to break when the cap or lid is moved. Sample collectors should: Sign and date the sample custody seal using waterproof ink, usually a 1- by 3-inch white paper label with black lettering and an adhesive backing. The custody seal is part of the COC process and is used to prevent or identify tampering with samples. Place the custody seal across the container lid so that the seal will be broken if a container is opened. If a cooler is used, ensure that the water drainage point is secure. Alternatively, sample containers may be placed into a tamper-evident bag. Bags/seals with a unique identifier must be associated with the person collecting the sample through logbook entries, preservation of tear-off strips in the logbook, or similar means. When using a tape-type tamper-evident seal, the initials of the person securing the container and the date that it was secured must be recorded on the seal. 8 September 2017 ------- SAM Companion - Sample Collection Information Document 6.0 Definitions The following definitions are provided to describe the sample collection information listed in the tables in Attachments A, Bl, B2 and C: Analyte - The chemical compound, class of compound, radionuclide, radioactivity or biotoxin that will be analyzed/measured in the sample. The analytes in this document are intended to address the analytes listed in SAM 2017. Selected Analytical Method - The analytical methods selected by technical work group members to address the target analytes. Tiers are provided for the chemistry methods cited in Attachment A and the biotoxin methods cited in Attachment C, to indicate the level of method usability assigned for the specific analyte and sample type. - For chemistry methods, these tiers are described in SAM 2017 as: Tier I: Analyte/sample type is a target of the method(s). Data are available for all aspects of method performance and quality control measures supporting its use for analysis of environmental samples following a contamination incident. Evaluation and/or use of the method(s) in multiple laboratories indicate that the method can be implemented with no additional modifications for the analyte/sample type. Tier II: (1) The analyte/sample type is a target of the method(s) and the method(s) has been evaluated for the analyte/sample type by one or more laboratories, or (2) the analyte/sample type is not a target of the method(s), but the method has been used by laboratories to address the analyte/sample type. In either case, available data and/or information indicate that modifications will likely be needed for use of the method(s) to address the analyte/sample type. Tier III: The analyte/sample type is not a target of the method(s), and/or no reliable data supporting the method's fitness for its intended use are available. Data from other analytes or sample types, however, suggest that the method(s), with significant modification, may be applicable. - For biotoxin methods, these tiers are described in SAM 2017 as: Tier I: The biotoxin and sample type are both targets of the method(s). Data are available for all aspects of method performance and QC measures supporting its use without modifications. Tier II: The biotoxin is a target of the method, and the method has been evaluated by one or more laboratories. The sample type may or may not be a target of the method, and available data and/or information regarding sample preparation indicate that analyses of similar sample types were successful. However, additional testing and/or modifications may be needed. Tier III: The sample type is not a target of the method, and no reliable data supporting the method's fitness for its intended use are available. Data suggest, however, that the method(s) may be applicable with significant modification. Method types are provided for the radiochemistry methods cited in Attachment B1 and the biotoxin methods cited in Attachment C, to indicate the intended analytical purpose. - For radiochemistry methods, these method types are described in SAM 2017 as: 9 September 2017 ------- SAM Companion - Sample Collection Information Document Qualitative: The identified method is intended to determine the presence of a radionuclide. Although quantitative, these methods have been selected as qualitative methods since they can be utilized with shorter counting times, at greater uncertainty, when increased sample throughput and more rapid reporting of results are required. Confirmatory: The identified method is for measurement of the activity from a particular radionuclide per unit of mass, volume or area sampled. - For biotoxin methods, these method types are described in SAM 2017 as: Presumptive: Presumptive methods are generally more rapid than confirmatory methods, and might be used during the initial stages of remediation to evaluate the extent of contamination. Presumptive methods also might be used to identify samples that should be analyzed using the more extensive confirmatory methods, and can be adapted for large-scale sample analysis while maintaining an appropriate level of analytical certainty. Confirmatory: Confirmatory methods are generally more time consuming and expensive, and are intended to provide results with a higher level of certainty than those provided by presumptive methods. These methods should be considered for use when presumptive analysis indicates the presence of the biotoxin, a smaller subset of samples requires analysis, or as required for a tiered approach to remediation. Biological Activity: Methods that address biological activity tend to be time consuming and expensive, and are intended to provide a high level of certainty in corroborating results of presumptive and/or confirmatory assays. Depending on the goals of the remediation phase, biological activity methods may also be needed to determine bioactivity of a given biotoxin detected by a presumptive and/or confirmatory assay that responds to inactivated or non-viable biotoxin. Container - The type of container (e.g., bottle, bag) that must be used to hold the sample. The container must be sufficient to maintain sample integrity and be composed of materials that will remain inert when in contact with the sample. • Holding Time - The maximum amount of time allowable from sample collection until sample analysis or extraction. The actual holding time depends on the target analyte(s), the analytical method that will be used, and the data requirements for the contamination incident. Sample collectors should consult the analytical method and/or receiving laboratory for confirmation. Packaging - Sample container packaging requirements for shipment of samples to the laboratory. Preservation - Conditions and/or chemicals used to maintain the integrity of a sample. Some examples of common preservatives include nitric acid, hydrochloric acid, sodium thiosulfate, and temperatures in the range of 0-6 °C. The actual preservation requirements depend on the target analyte(s), the analytical method that will be used, and the data requirements for the contamination incident. Sample collectors should consult the analytical method and/or receiving laboratory for confirmation. Sample Size - The amount of sample that should be collected for a minimum of one analysis. The actual volume and weight requirements depend on the target analyte(s), the analytical method that will be used, and the data requirements for the contamination incident. Sample collectors should consult the analytical method and/or receiving laboratory for confirmation. 10 September 2017 ------- SAM Companion - Sample Collection Information Document Sample Type - The type of material to be collected for analysis (e.g., air, drinking water, non-drinking water, solid, soil and sediment, wipe, vegetation). In cases where the same analytical method(s) is indicated for analysis of both drinking and non-drinking water sample types, the sample types and corresponding sample collection information are combined into a single row. Sampling Device/Medium - The device or medium used to collect samples to be analyzed for biotoxins. Shipping Label - U.S. DOT shipping label requirements under 49 CFR 172 and 173. 7.0 Bibliography Analytical methods listed in Attachments A, Bl, B2 and C can be accessed through the EPA Homeland Security Research Program's Sampling and Analytical Methods website at https://www.epa.gov/homeland-securitv-research/sam. In addition to these methods, the following resources were used to prepare this document: • U.S. Centers for Disease Control and Prevention. December 2009. Biosafety in Microbiological and Biomedical Laboratories, 5th Edition. https://www.cdc.gov/biosafetv/publications/bmbl5/bmbl.pdf (accessed September 2017) U.S. Department of Energy. March 1979. "Radiochemical Analytical Procedures for Analysis of Environmental Samples." Environmental Monitoring and Support Laboratory. EMSL-LV- 0539-17. http://www.health.state.mn.us/divs/phl/accreditation/docs/refmethodepa053917.pdf (accessed September 2017). U.S. Department of Labor. 29 CFR 1910.120, "Hazardous Waste Operations and Emergency Response," 29 CFR 1910.120, Appendix B, "General Description and Discussion of the Levels of Protection and Protective Gear," and 1910.38 "Emergency Action Plans." http://www.osha.gov/pls/oshaweb/owasrch.search form?p doc tvpe=STANDARDS&p toe level=l&p kewalue=1910 (accessed September 2017). U.S. Department of Labor. 29 CFR 1926.65. "Hazardous Waste Operations and Emergency Response." http://www.osha.gov/pls/oshaweb/owadisp.show document?p table=STANDARDS&p id=l 0651 (accessed September 2017). U.S. Department of Transportation. 49 CFR 172.101. "Purpose and Use of Hazardous Materials Table." http://www.ecfr.gov/cgi-bin/text- idx?tpl=/ecfrbrowse/Title49/49cfrl72 main 02.tpl (accessed September 2017). • U.S. Department of Transportation. 49 CFR 172.301. "General Marking Requirements for Non-bulk Packagings." http://www.ecfr.gov/cgi-bin/text- idx?tpl=/ecfrbrowse/Title49/49cfrl72 main 02.tpl (accessed September 2017). U.S. Department of Transportation. 49 CFR 173. "Shippers - General Requirements for Shipments and Packaging." http://www.ecfr.gov/cgi-bin/text- idx?tpl=/ecfrbrowse/Title49/49cfrl73 main 02.tpl (accessed September 2017). - 49 CFR 173.132. "Class 6, Division 6.1-Definitions." http://www.ecfr.gov/cgi-bin/text- 11 September 2017 ------- SAM Companion - Sample Collection Information Document idx'.)SID=dfbaf3da73f9ccbc9c9bclacdS52()d97&mc=truc&nodc=sc49.2.173 1 132&rgn= div8 (accessed September 2017). - 49 CFR 173.150. "Exceptions for Class 3 (flammable and combustible liquids)." http://www.ecfr.gov/cgi-bin/text- idx?SID=dfbaf3da73f9cebe9c9bclaed8520d97&mc=true&node=se49.2.173 1150&rgn= div8 (accessed September 2017). - 49 CFR 173.153. "Exceptions for Division 6.1 (poisonous materials)." http://www.ecfr.gov/cgi-bin/text- idx?SID=dfbaf3da73f9cebe9c9bclaed8520d97&mc=true&node=se49.2.173 1153&rgn= div8 (accessed September 2017). - 49 CFR 173.154. "Exceptions for Class 8 (corrosive materials)." http://www.ecfr.gov/cgi-bin/text- idx?SID=dfbaf3da73f9cebe9c9bclaed8520d97&mc=true&node=se49.2.173 1154&rgn= div8 (accessed September 2017). - 49 CFR 173.24 and 173.24a. "General Requirements for Packaging" and "Packages and Additional General Requirements for Non-bulk Packaging and Packages." http://www.ecfr.gov/cgi-bin/text- idx?SID=ee650c0b6877d30fc0c3bb9637a4b9c3&mc=true&node=se49.2.173 124&rgn= div8 (accessed September 2017) and http://www.ecfr.gov/cgi-bin/text- idx?SID=ee650c0b6877d30fc0c3bb9637a4b9c3&mc=true&node=se49.2.173 124a&rgn =div8 (accessed September 2017). - 49 CFR 173.403. "Definitions." http://www.ecfr.gov/cgi-bin/text- idx?SID=dfbaf3da73f9cebe9c9bclaed8520d97&mc=true&node=se49.2.173 1403&rgn= div8 (accessed September 2017). - 49 CFR 173.453. "Fissile Materials - Exceptions." http://www.ecfr.gov/cgi-bin/text- idx?SID=dfbaf3da73f9cebe9c9bclaed8520d97&mc=true&node=se49.2.173 1453&rgn= div8 (accessed September 2017). • U.S. Environmental Protection Agency. 40 CFR 61, Appendix B. "Standards of Performance for New Stationary Sources, Performance Specifications - Test Methods." http://www.ecfr.gov/cgi-bin/text- idx?SID=4ac3 5 9277bd961623 7111031429 le70b&node=40:9.0.1.1.1.29.1.21,5&rgn=div9 (accessed September 2017). U.S. Environmental Protection Agency. July 2012. "Sample Collection Procedures for Radiochemical Analytes in Environmental Matrices." EPA/600/R-12/566, National Homeland Security Research Center. Cincinnati, OH. https://www.epa.gov/sites/production/files/2015- 07/documents/sample collection procedures for radiochemical analvtes.pdf (accessed September 2017). U.S. Environmental Protection Agency. September 2016. "Sample Collection Procedures for Radiochemical Analytes in Outdoor Building and Infrastructure Materials." EPA/600/R- 16/128, National Homeland Security Research Center. Cincinnati, OH. https://cfpub.epa.gov/si/si public record report.cfm?dirEntrvId=335065 (accessed September 2017). 12 September 2017 ------- SAM Companion - Sample Collection Information Document U.S. Environmental Protection Agency. Office of Solid Waste and Emergency Response (OSWER), SW-846 Methods on Line. http://www.epa.gov/osw/hazard/testmethods/sw846/online/ (accessed September 2017). U.S. Environmental Protection Agency. Office ofWater. February 2017. Sampling Guidance for Unknown Contaminants in Drinking Water. EPA-817-R-08-003. https://www.epa.gov/waterlabnetwork/sampling-guidance-unknown-contaminants-drinking- water (accessed September 2017) U.S. Office of Management and Budget. January 27, 2016. 81 Federal Register 4673: Revisions of OMB Circular No. A-l 19 "Federal Participation in the Development and Use of Voluntary Consensus Standards and in Conformity Assessment Activities." https://www.gpo.gov/fdsys/pkg/FR-2016-01-27/pdf/2016-01606.pdf (accessed September 2017). * U.S. Environmental Protection Agency. September 2017. Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2017. EPA/600/R-17/356, National Homeland Security Research Center. Cincinnati, OH. https://www.epa.gov/homeland- security-research/sam (accessed September 2017). 13 September 2017 ------- This page left intentionally blank ------- Attachment A Sample Collection Information for the Chemical Analytes and Methods Listed in SAM 2017 [Note: The acronyms and abbreviations used in this attachment are listed and defined in the beginning of the report.] ------- This page left intentionally blank ------- Sample Collection Information Document - Attachment A September 2017 Attachment A: Sample Collection Information for the Chemical Analytes and Methods Listed in SAM 2017 Note: In some cases, analytes are listed as not a concern in a particular sample type; in these cases, SAM work groups have determined that the analyte is not a concern due to a number of factors, including low likelihood of persistence, toxicity, mobility or solubility. Analyte Selected Analytical Methods -SAM2017* Sample Type Sample Size<1) Sample Container*2' Holding Time Preservation Packaging Requirements Shipping Label131 Acephate J. Chromatogr. A (2007) 1154(1): 3-25 [Tier III] Air TBD TBD Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Chromatographia, 63(5/6): 223-237 [Tier II] Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 538 (EPA OW) [Tier I] Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Prior to collection, add 400 pL of ammonium acetate concentrated stock and 80 [jL of concentrated sodium omadine stock to sample container [Concentrations of ammonium acetate and sodium omadine in the sample should be 1.5 g/L and 64 mg/L, respectively.] • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 J. Chromatogr. A (2007) 1154(1): 3-25 [Tier II] Solid 250 mL Wide-mouth glass or fluoropolymer container with fluoropolymer-lined lid Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 J. Chromatogr. A (2007) 1154(1): 3-25 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth glass or fluoropolymer container with fluoropolymer-lined lid Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 SAM Companion - Sample Collection Information Document A1-1 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Acrylamide PV2004 (OSHA) [Tier I] Air 120 L (flow rate = 1 L/min) Filter/solid sorbent tube (13mm quartz fiber filter) • OVS-7 tube (XAD-7 resin) • OVS-2 (XAD-2 resin) • OVS-SG (silica gel) 13 days Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Acrylamide, solid, 6.1, Poison, UN2074 8316 (EPA SW-846) [Tier II] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the bottle with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Acrylamide solution, 6.1, Poison, UN3426 Water Extraction / 8316 (EPA SW-846) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septa or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the bottle with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Acrylamide, solid, 6.1, Poison, UN2074 3570 / 8290A Appendix A / 8316 (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Acrylamide, solid, 6.1, Poison, UN2074 Acrylonitrile PV2004 (OSHA) [Tier III] Air 120 L (flow rate = 1 L/min) Filter/solid sorbent tube (13mm quartz fiber filter) • OVS-7 tube (XAD-7 resin) • OVS-2 (XAD-2 resin) • OVS-SG (silica gel) 13 days Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Acrylonitrile, stabilized, 3, Flammable liquid, 6.1, Poison, UN1093 524.2/524.3/524.4 (EPA OW) [Tier II] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer screw cap VOA vial with fluoro polymer-lined septum, filled with no headspace 14 days with acidification; 24 hours if not acidified Prior to collection of treated water samples, add 25 mg ascorbic acid to container for removal of residual chlorine Method 524.2: Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Acrylonitrile, stabilized, 3, Flammable liquid, 6.1, Poison, UN1093 • Adjust to pH < 2 with 1:1 HCI Methods 524.3 and 524.4: • Adjust to pH < 2 with maleic acid For all samples: • If residual chlorine is > 5 mg/L, add an additional 25 mg ascorbic acid per each 5 mg/L chlorine • Cool to < 6°C; keep from freezing 5035A / 8260D (EPA SW-846) [Tier II] Solid 5-g coring device or amber VOA vial AND 1 - 2 oz. glass jar (if needed to determine percent moisture) Coring device (If coring device is to be cleaned and reused, samples can be transferred to an amber VOA vial.) AND glass jar (if needed to determine percent moisture) 14 days if frozen; 48 hours if not frozen Cool to <6°C; freeze within 48 hours to < negative (-) 7°C [Coring tools should not be frozen below -20°C.] Wipe outside of coring device or container clean using a damp, then dry cloth. Place the coring device or container in a zipper bag. Pack samples as described in Footnote (4). Standard carrier shipping label AND Acrylonitrile, stabilized, 3, Flammable liquid, 6.1, Poison, UN1093 SAM Companion - Sample Collection Information Document A1-2 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 3570 /8290A Appendix A/ 8260D (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Acrylonitrile, stabilized, 3, Flammable liquid, 6.1, Poison, UN1093 Aldicarb (Temik) 5601 (NIOSH) [Tier I] Air 240 - 480 L (flow rate = 0.1 - 1 L/min) Filter/solid sorbent tube (OVS- 2 tube: 13 mm quartz fiber filter, XAD-2 [270 mg/140 mg]) 30 days if frozen; 7 days at room temperature (24°C) Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 D7645-16 (ASTM) [Tier II] Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid 14 days • Acidify to pH < 3.8 with glacial acetic acid • Cool to < 6°C; keep from freezing • Prior to collection of treated water samples, add 10 mg ascorbic acid to sample container Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 531.2 (EPA OW) [Tier I] Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid 28 days • Prior to collection, add 0.4 g potassium dihydrogen citrate and 3.2 - 4.8 mg sodium thiosulfate to sample container • Agitate for 1 minute after collection • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 8318A (EPA SW-846) [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 3570 / 8290A Appendix A / 8318A (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 SAM Companion - Sample Collection Information Document A1-3 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Aldicarb sulfone 5601 (NIOSH) [Tier III] Air 240 - 480 L (flow rate = 0.1 - 1 L/min) Filter/solid sorbent tube (OVS- 2 tube: 13 mm quartz filter, XAD-2, [270 mg/140 mg]) 30 days if frozen; 7 days at room temperature (24°C) Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 D7645-16 (ASTM) [Tier II] Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Acidify to pH < 3.8 with glacial acetic acid • Cool to < 6°C; keep from freezing • Prior to collection of treated water samples, add 10 mg ascorbic acid to sample container Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 531.2 (EPA OW) [Tier I] Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 28 days • Prior to collection, add 0.4 g potassium dihydrogen citrate and 3.2 - 4.8 mg sodium thiosulfate to sample container • Agitate for 1 minute after collection • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 8318A (EPA SW-846) [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 3570/8290A Appendix A/ 8318A (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 SAM Companion - Sample Collection Information Document A1-4 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -SAM2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Aldicarb sulfoxide 5601 (NIOSH) [Tier III] Air 240 - 480 L (flow rate = 0.1 - 1 L/min) Filter/solid sorbent tube (OVS- 2 tube: 13 mm quartz filter, XAD-2, [270 mg/140 mg]) 30 days if frozen; 7 days at room temperature (24°C) Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 D7645-16 (ASTM) [Tier II] Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Acidify to pH < 3.8 with glacial acetic acid • Cool to < 6°C; keep from freezing • Prior to collection of treated water samples, add 10 mg ascorbic acid to sample container Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 531.2 (EPA OW) [Tier I] Drinking Water 40 mL Amber glass or fluoropolymer container with fIuoropolymer- lined septum or lid 28 days • Prior to sample collection, add 0.4 g potassium dihydrogen citrate and 3.2 - 4.8 mg sodium thiosulfate to sample container • Agitate for 1 minute after collection • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 8318A (EPA SW-846) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 3570 / 8290A Appendix A / 8318A (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 Allyl alcohol TO-15 (EPA ORD) [Tier III] Air 1-Lor 6-L canister (depending on suspected concentration)'6' Specially prepared stainless steel canister For subatmospheric pressure sampling, the canister is evacuated to 0.05 mm Hg; for pressurized sampling, the sample is collected using a pump and flow control arrangement to achieve a typical 101 - 202 kPa (15-30 psig) 30 days None Ship canister in container provided by laboratory Standard carrier shipping label AND Allyl alcohol, 3, Flammable liquid, 6.1, Poison, UN1098 5030C / 8260D (EPA SW-846) [Tier II] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer screw cap VOA vial with fluoro polymer-lined septum, filled with no headspace 14 days with acidification; 7 days if not acidified • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Adjust to pH < 2 with 1:1 HCI • Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap glass bottles with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Allyl alcohol, 3, Flammable liquid, 6.1, Poison, UN1098 5035A / 8260D (EPA SW-846) [Tier II] Solid 5-g coring device or amber VOA vial AND 1 - 2 oz. glass jar (if needed to determine percent moisture) Coring device (If coring device is to be cleaned and reused, samples can be transferred to an amber VOA vial.) AND glass jar (if needed to determine percent moisture) 14 days if frozen; 48 hours if not frozen Cool to <6°C; freeze within 48 hours to < negative (-) 7°C [Coring tools should not be frozen below -20 °C.] Wipe outside of coring device or container clean using a damp, then dry cloth. Place the coring device or container in a zipper bag. Pack samples as described in Footnote (4). Standard carrier shipping label AND Allyl alcohol, 3, Flammable liquid, 6.1, Poison, UN1098 NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-5 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 4-Aminopyridine NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3535A / 8330B (EPA SW-846) [Tier III] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Aminopyridines, 6.1, Poison, UN2671 8330B (EPA SW-846) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septum or lid 14 days Cool to< 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Aminopyridines, 6.1, Poison, UN2671 3570 / 8290A Appendix A / 8330B (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Aminopyridines, 6.1, Poison, UN2671 Ammonia 6015 (NIOSH) [Tier I] Air 0.1 (at 50 ppm) - 96 L (flow rate = 0.1 - 0.2 L/min) Solid sorbenttube (sulfuric acid-treated silica gel) Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of sorbenttube clean using a damp, then dry cloth. Cap tube with plastic (not rubber) caps immediately after sampling. Place tubes in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND (if greater than 10% ammonia by volume) Ammonia solution, 8, Corrosive, UN2672 4500-NH3B/ 4500- NH3G (SM) [Tier I] Non-Drinking Water 500 mL Polyethylene or glass container 28 days with acidification; analyze immediately / as soon as possible if not acidified • Cool to < 6°C or acidify to pH < 2; keep from freezing • Add sodium thiosulfate to remove residual chlorine from treated water samples (5) (Can be added in the field or upon receipt in the laboratory.) Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND (if greater than 10% ammonia by volume) Ammonia solution, 8, Corrosive, UN2672 350.1 (EPA OW) [Tier I] Drinking Water 500 mL Polyethylene or glass container 28 days • Add sodium thiosulfate to remove residual chlorine from treated water samples 151 (Can be added in the field or upon receipt in the laboratory.) • Adjust to pH < 2 with H2S04 • Cool to< 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND (if greater than 10% ammonia by volume) Ammonia solution, 8, Corrosive, UN2672 NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-6 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container*2' Holding Time Preservation Packaging Requirements Shipping Label131 Ammonium metavanadate (analyze as total vanadium) 10-3.1 / 10-3.4/ 10-3.5 (EPA ORD) [Tier 1] Air Up to 1700 m3 Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ammonium metavanadate, 6.1, Poison, UN2859 3015A / 6010D/6020B (EPA SW-846) [Tier I] Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ammonium metavanadate, 6.1, Poison, UN2859 200.7 / 200.8 (EPA OW) [Tier I] Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ammonium metavanadate, 6.1, Poison, UN2859 3050B / 3051A / 6010D / 6020B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container Analyze immediately / as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ammonium metavanadate, 6.1, Poison, UN2859 9102 (NIOSH)/ 6010D / 6020B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container Analyze immediately / as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ammonium metavanadate, 6.1, Poison, UN2859 Arsenic trioxide (analyze as total arsenic) IO-3.1 / 10-3.4/ 10-3.5 (EPA ORD) [Tier 1] Air Up to 1700 m3 Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsenic trioxide, 6.1, Poison, UN1561 3015A / 6010D / 6020B (EPA SW-846) [Tier I] Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsenic trioxide, 6.1, Poison, UN1561 200.7 / 200.8 (EPA OW) [Tier I] Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsenic trioxide, 6.1, Poison, UN1561 3050B / 3051 A/ 6010D / 6020B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsenic trioxide, 6.1, Poison, UN1561 9102 (NIOSH)/ 6010D / 6020B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsenic trioxide, 6.1, Poison, UN1561 SAM Companion - Sample Collection Information Document A1-7 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container*2' Holding Time Preservation Packaging Requirements Shipping Label131 Arsenic, Total 10-3.1 / 10-3.4/ 10-3.5 (EPA ORD) [Tier 1] Air Up to 1700 m3 Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsenic, 6.1, Poison, UN1558 3015A / 6010D / 6020B (EPA SW-846) [Tier I] Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsenic, 6.1, Poison, UN1558 200.7 / 200.8 (EPA OW) [Tier I] Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsenic, 6.1, Poison, UN1558 3050B / 3051A / 6010D / 6020B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsenic, 6.1, Poison, UN1558 9102 (NIOSH)/ 6010D / 6020B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsenic, 6.1, Poison, UN1558 Arsine (analyze as total arsenic in non-air samples) 6001 (NIOSH) [Tier I] Air 0.1 L (at 0.05 ppm) - 10 L (flow rate = 0.01 -0.2 L/min) Solid sorbenttube (coconut shell charcoal [100 mg/50 mg]) 6 days Cool to < 6°C Wipe outside of sorbenttube clean using a damp, then dry cloth. Cap tubes with plastic (not rubber) caps. Place tubes in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsine, 2.1, Flammable gas, 2.3, Poisonous gas, UN2188 3015A / 6010D / 6020B (EPA SW-846) [Tier I] Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsine, 2.1, Flammable gas, 2.3, Poisonous gas, UN2188 200.7 / 200.8 (EPA OW) [Tier I] Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsine, 2.1, Flammable gas, 2.3, Poisonous gas, UN2188 3050B / 3051A / 6010D / 6020B (EPA SW-846) [Tier I] Solid 40 mL Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsine, 2.1, Flammable gas, 2.3, Poisonous gas, UN2188 9102 (NIOSH)/ 6010D / 6020B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Arsine, 2.1, Flammable gas, 2.3, Poisonous gas, UN2188 SAM Companion - Sample Collection Information Document A1-8 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Asbestos 10312:1995 (ISO) [Tier I] Air 500 - 5000 L (flow rate = 1 -10 L/min) Polycarbonate or cellulose ester filter Samples can be held indefinitely None Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Asbestos, 9, Class 9, NA2212 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type D5755-03 (soft surfaces-micro vac) (ASTM) [Tier III] Solid 200 mL Wide-mouth, sealable glass or fluoropolymer container Samples can be held indefinitely Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Asbestos, 9, Class 9, NA2212 D6480-05 (ASTM) [Tier I] Wipes 1 particle-free wipe/100 cm2 Wide-mouth, sealable glass or fluoropolymer container Samples can be held indefinitely Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Asbestos, 9, Class 9, NA2212 Boron trifluoride ID216SG (OSHA) [Tier I] Air Up to 480 L (flow rate = 1 L/min) Midget glass bubbler containing 10 mL of 0.1 M NH4F; sample solution transferred to 20 mL glass vial with fluoropolymer-lined cap Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Boron trifluoride, 2.3, Poisonous gas, UN1008 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type Brodifacoum NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type D7644-16 (ASTM) [Tier II] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Add 10 mg of dry ascorbic acid to each 40 mL vial prior to collection • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Coumarin derivative pesticides, liquid, toxic, 6.1, Poison, UN3023 3541 /3545A (EPA SW-846)/ D7644-16 (ASTM) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septa or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Coumarin derivative pesticides, solid, toxic, 6.1, Poison, UN3026 3570/8290A Appendix A (EPA SW-846)/ D7644-16 (ASTM) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Coumarin derivative pesticides, solid, toxic, 6.1, Poison, UN3026 SAM Companion - Sample Collection Information Document A1-9 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Bromadiolone NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type D7644-16 (ASTM) [Tier II] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Add 10 mg of dry ascorbic acid to each 40 mL vial prior to collection • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Coumarin derivative pesticides, liquid, toxic, 6.1, Poison, UN3023 3541 /3545A (EPA SW-846)/ D7644-16 (ASTM) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Coumarin derivative pesticides, solid, toxic, 6.1, Poison, UN3026 3570/8290A Appendix A (EPA SW-846)/ D7644-16 (ASTM) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Coumarin derivative pesticides, solid, toxic, 6.1, Poison, UN3026 [BZ] Quinuclidinyl benzilate TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent Analyze as soon as possible until holding time are available Cool to < 6°C Wrap cartridge in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. J. Chromatogr. B (2008) 874: 42-50 [Tier III] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fiuoro polymer- lined septum or lid Analyze as soon as possible until holding time data are available Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. 3541/3545A (EPA SW-846) / J. Chromatogr. B (2008) 874: 42-50 [Tier III] Solid 40 mL Amber glass or fluoropolymer container with fiuoro polymer- lined septum or lid Analyze as soon as possible until holding time data are available Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. 3570/8290A Appendix A (EPA SW-846) / J. Chromatogr. B (2008) 874: 42-50 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid Analyze as soon as possible until holding time data are available Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. SAM Companion - Sample Collection Information Document A1-10 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container*2' Holding Time Preservation Packaging Requirements Shipping Label131 Calcium arsenate (analyze as total arsenic) 10-3.1 / 10-3.4/ 10-3.5 (EPA ORD) [Tier 1] Air Up to 1700 m3 Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Calcium arsenate, 6.1, Poison, UN 1573 3015A / 6010D / 6020B (EPA SW-846) [Tier I] Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Calcium arsenate, 6.1, Poison, UN 1573 200.7 / 200.8 (EPA OW) [Tier I] Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Calcium arsenate, 6.1, Poison, UN 1573 3050B / 3051A / 6010D / 6020B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Calcium arsenate, 6.1, Poison, UN 1573 9102 (NIOSH)/ 6010D / 6020B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Calcium arsenate, 6.1, Poison, UN 1573 SAM Companion - Sample Collection Information Document A1-11 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Carbofuran (Furadan) 5601 (NIOSH) [Tier I] Air 240 - 480 L (flow rate = 0.1 - 1 L/min) Filter/solid sorbent tube (OVS- 2 tube: 13 mm quartz fiber filter, XAD-2 [270 mg/140 mg]) 30 days if frozen; 7 days at room temperature (24°C) Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 D7645-16 (ASTM) [Tier II] Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Acidify to pH < 3.8 with glacial acetic acid • Cool to < 6°C; keep from freezing • Prior to collection of treated water samples, add 10 mg ascorbic acid to sample container Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 531.2 (EPA OW) [Tier I] Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 28 days • Prior to collection, add 0.4 g potassium dihydrogen citrate and 3.2 - 4.8 mg sodium thiosulfate to sample container • Agitate for 1 minute after collection • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 8318A (EPA SW-846) [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 3570 / 8290A Appendix A / 8318A (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 SAM Companion - Sample Collection Information Document A1-12 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Carbon disulfide TO-15 (EPA ORD) [Tier I] Air 1-L or 6-L canister (depending on suspected concentration)'6' Specially prepared stainless steel canister For subatmospheric pressure sampling, the canister is evacuated to 0.05 mm Hg; for pressurized sampling, the sample is collected using a pump and flow control arrangement to achieve a typical 101 - 202 kPa (15-30 psig) 30 days None Ship canister in container provided by laboratory Standard carrier shipping label AND Carbon disulfide, 3, Flammable liquid, 6.1, Poison, UN 1131 5030C / 8260D (EPA SW-846) [Tier I] Non-Drinking Water 40 ml. Screw cap VOA vial with fluoropolymer-lined septum, filled with no headspace 14 days with acidification; 7 days if not acidified • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Adjust to pH < 2 with 1:1 HCI • Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbon disulfide, 3, Flammable liquid, 6.1, Poison, UN 1131 524.2/524.3/524.4 (EPA OW) [Tier I] Drinking Water 40 ml. Amber glass or fluoropolymer screw cap VOA vial with fluoro polymer-lined septum, filled with no headspace 14 days with acidification; 24 hours if not acidified Prior to collection of treated water samples, add 25 mg ascorbic acid to container for removal of residual chlorine Method 524.2: Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbon disulfide, 3, Flammable liquid, 6.1, Poison, UN 1131 • Adjust to pH < 2 with 1:1 HCI Methods 524.3 and 524.4: • Adjust to pH < 2 with maleic acid For all samoles: • If residual chlorine is > 5 mg/L, add an additional 25 mg ascorbic acid per each 5 mg/L chlorine • Cool to < 6°C; keep from freezing 5035A / 8260D (EPA SW-846) [Tier I] Solid 5-g coring device or amber VOA vial AND 1 - 2 oz. glass jar (if needed to determine percent moisture) Coring device (If coring device is to be cleaned and reused, samples can be transferred to an amber VOA vial.) AND glass jar (if needed to determine percent moisture) 14 days if frozen; 48 hours if not frozen Cool to < 6°C; freeze within 48 hours to < negative (-) 7°C [Coring tools should not be frozen below-20°C.] Wipe outside of coring device or container clean using a damp, then dry cloth. Place the coring device or container in a zipper bag. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbon disulfide, 3, Flammable liquid, 6.1, Poison, UN 1131 NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-13 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Carfentanil NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3520C / 3535A (EPA SW-846) / J. Chromatogr. B (2014) 962: 52-58 [Tier III] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organic, n.o.s., 6.1, toxic, UN2810 3541 /3545A (EPA SW-846) J. Chromatogr. B (2014) 962: 52-58 [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organic, n.o.s., 6.1, toxic, UN2810 3570 /8290A Appendix A (EPA SW-846)/ J. Chromatogr. B (2014) 962: 52-58 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organic, n.o.s., 6.1, toxic, UN2810 Chlorfenvinphos TO-10A (EPA ORD) [Tier II] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3520C/3535A / 8270E (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Chlorine Analyst (1999) 124(12): 1853- 1857/ 4500-CI G (SM) [Tier II] Air 8 L (at 0.1 ppm)- 360 L (flow rate = 0.039 - 2.94 L/min) Prefilter and filter (fluoropolymer, 0.5 |jm and silver membrane, 25 mm, 0.45 Mm) 30 days Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Chlorine, 2.3, 5.1, Oxidizer, 8, Poison gas, UN1017 4500-CI G (SM) [Tier I] Drinking Water Non-Drinking Water 500 mL Amber glass or fluoropolymer container Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Avoid excessive agitation Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Chlorine, 2.3, 5.1, Oxidizer, 8, Poison gas, UN1017 NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-14 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 2-Chloroethanol 2513 (NIOSH) [Tier I] Air 2 (at 5 ppm) - 35 L (flow rate = 0.01 - 0.2 L/min) Solid sorbenttube (petroleum charcoal [100 mg/50 mg]) 14 days Cool to < 6°C Wipe outside of sorbenttube clean using a damp, then dry cloth. Cap tubes with plastic (not rubber) caps. Place tubes in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethylene chlorohydrin, 3, Flammable liquid, 6.1 Poison, UN 1135 5030C / 8260D (EPA SW-846) [Tier II] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer screw cap VOA vial with fluoropolymer-lined septum, filled with no headspace 14 days with acidification; 7 days if not acidified • Add sodium thiosulfate to remove residual chlorine from treated water samples.15' • Adjust to pH < 2 with 1:1 HCI • Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethylene chlorohydrin, 3, Flammable liquid, 6.1 Poison, UN 1135 5035A / 8260D (EPA SW-846) [Tier II] Solid 5-g coring device or amber VOA vial AND 1 - 2 oz. glass jar (if needed to determine percent moisture) Coring device (If coring device is to be cleaned and reused, samples can be transferred to an amber VOA vial.) AND glass jar (if needed to determine percent moisture) 14 days if frozen; 48 hours if not frozen Cool to < 6°C; freeze within 48 hours to < negative (-) 7°C [Coring tools should not be frozen below-20°C.] Wipe outside of coring device or container clean using a damp, then dry cloth. Place the coring device or container in a zipper bag. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethylene chlorohydrin, 3, Flammable liquid, 6.1 Poison, UN 1135 NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type Chloropicrin PV2103 (OSHA) [Tier I] Air 5 L (flow rate = 0.2 L/min) Solid sorbenttube (XAD-4 [100 mg/50 mg]) Until holding time data are available, analyze immediately/ as soon as possible • Cool to < 6°C • Protect from light Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap tubes with plastic (not rubber) caps. Place tubes in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Chloropicrin, 6.1, Poison, UN 1580 551.1 (EPA OW) [Tier I] Drinking Water Non-Drinking Water 60 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C; keep from freezing • Prior to collection of treated water samples, add 6 mg NH4CI to sample container Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Chloropicrin, 6.1, Poison, UN 1580 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Chloropicrin, 6.1, Poison, UN 1580 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool 2 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Chloropicrin, 6.1, Poison, UN 1580 SAM Companion - Sample Collection Information Document A1-15 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 3-Chloro-1,2-propanediol TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Glycerol alpha- monochlorohydrin, 6.1, Toxic substance, UN2689 J. Chromatogr. A (2000) 866: 65-77 [Tier II] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 15 days • Adjust pH to 3 - 8 with sodium sulfite or 1:1 HCI • Cool to <°6; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Glycerol alpha- monochlorohydrin, 6.1, Toxic substance, UN2689 Eur. J. Lipid Sci. Technol. (2011) 113: 345-355 [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Glycerol alpha- monochlorohydrin, 6.1, Toxic substance, UN2689 Eur. J. Lipid Sci. Technol. (2011) 113: 345-355 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Glycerol alpha- monochlorohydrin, 6.1, Toxic substance, UN2689 Chlorosarin TO-17 (EPA ORD) [Tier III] Air 1 -4 L (flow rate = 16-67 m L/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier III] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid 7 days • Cool to < 6°C; keep from freezing • Add sodium thiosulfate to remove residual chlorine from treated water samples(5) Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier III] Solid 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. SAM Companion - Sample Collection Information Document A1-16 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Chlorosoman TO-17 (EPA ORD) [Tier III] Air 1 -4 L (flow rate = 16-67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier III] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier III] Solid 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. 2-Chlorovinyl arsonic acid (CVAOA) (degradation product of Lewisite) 10-3.1 IO-3.4/IO-3.5 (EPA ORD) [Tier I] Air Up to 1700 m3 Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. E PA/60 O/R-15/258 (EPA NHSRC) [Tier II] Drinking Water Non-Drinking Water 40 ml. VOA vial with fluoropolymer- lined cap 14 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-15/258 (EPA NHSRC) [Tier II] Solid 40 ml. VOA vial with fluoropolymer- lined cap Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/60 O/R-15/258 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 VOA vial with fluoropolymer- lined cap 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. SAM Companion - Sample Collection Information Document A1-17 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 2-Chlorovinylarsonous acid (2- CVAA) (degradation product of Lewisite) 10-3.1 IO-3.4/IO-3.5 (EPA ORD) [Tier I] Air Up to 1700 m" Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-15/258 (EPA NHSRC) [Tier II] Drinking Water Non-Drinking Water 40 ml. VOA vial with fluoropolymer- lined cap 14 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-15/258 (EPA NHSRC) [Tier II] Solid 40 ml. VOA vial with fluoropolymer- lined cap Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-15/258 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 VOA vial with fluoropolymer- lined cap 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. SAM Companion - Sample Collection Information Document A1-18 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Chlorpyrifos TO-10A (EPA ORD) [Tier I] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Non-Drinking Water 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days For treated water samoles: Preservatives/dechlorinating agents not required, but the analyte is not affected by the following, if added to address other analytes: • 1.6-2.0 mg NH4CI For all other samoles: • Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 525.2 / 525.3 (EPA OW) [Tier I] Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days Method 525.2: • Add sodium sulfite to empty sample container to remove residual chlorine from treated water samples • Acidify to pH < 2 with 1:1 HCI(a • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 Method 525.3 Cto emotv sample container): • Add 4 mg ascorbic acid to remove residual chlorine from treated water samples • Add 14 mg trisodium EDTA, and 400 mg potassium dihydrogen citrate • Cool to < 6°C; keep from freezing EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 SAM Companion - Sample Collection Information Document A1-19 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Chlorpyrifos oxon TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 540 (EPA OW) [Tier I] Drinking Water Non-Drinking Water 250 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Prior to collection, add solid ascorbic acid (dechlorinating agent), Trizma® base (buffering agent) and chloroacetamide (antimicrobial preservative) at concentrations of 7.75 g/L, 2 g/L and 100 mg/L, respectively to an empty sample container • After collecting the sample, cap the container and agitate until preservative is dissolved • Cool samples to <10°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Crimidine NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type EPA/600/R-16/114 (EPA NHSRC) [Tier II] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days For treated water samoles: Preservatives/dechlorinating agents not required, but the analyte is not affected by the following, if added to address other analytes: •2.0 mg NH4CI • Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Pesticide, n.o.s., liquid, 6.1, Poison, UN2902 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Pesticide, n.o.s., solid, 6.1, Poison, UN2588 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Pesticide, n.o.s., solid, 6.1, Poison, UN2588 SAM Companion - Sample Collection Information Document A1-20 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Cyanide, Amenable to chlorination NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3135.21 (EPA Regional Lab) [Tier 1] Drinking Water Non-Drinking Water 1 L Glass or polyethylene container 14 days For treated water samoles: • Add 0.6 g of ascorbic acid for each liter of sample volume For all samoles: • Adjust to pH > 12 with NaOH • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Cyanide solutions n.o.s, 6.1, Poison, UN1935 3135.21 (EPA Regional Lab) [Tier 1] Solid 250 mL Wide-mouth glass or polyethylene container 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Cyanide inorganic solid n.o.s, 6.1, Poison, UN1588 3135.21 (EPA Regional Lab) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth glass or polyethylene container 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Cyanide inorganic solid n.o.s, 6.1, Poison, UN1588 Cyanide, Total 6010 (NIOSH) [Tier I] Air 2 (at 5 ppm) - 90 L (flow rate = 0.05 - 0.2 L/min) Solid sorbenttube (soda lime [600 mg/200 mg]) 14 days Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap tubes with plastic (not rubber) caps. Place tubes in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Cyanides, inorganic, solid, n.o.s, 6.1, Poison, UN1588 ISM 02.3 CN (EPA CLP) [Tier I] Non-Drinking Water 500 mL Amber glass or polyethylene container 14 days For treated water samoles: '• Add 0.6 g of ascorbic acid for each liter of sample volume For all samoles: • Adjust to pH > 12 with NaOH • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Cyanide solutions n.o.s, 6.1, Poison, UN1935 335.4 (EPA OW) [Tier I] Drinking Water 125 mL Amber glass or polyethylene container 14 days • Add 0.6 g of ascorbic acid for each liter of sample volume • Adjust to pH > 12 with NaOH • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Cyanide solutions n.o.s, 6.1, Poison, UN1935 ISM 02.3 CN (EPA CLP) [Tier I] Solid 40 mL Wide-mouth amber glass or polyethylene container 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Cyanides, inorganic, solid, n.o.s, 6.1, Poison, UN1588 ISM 02.3 CN (EPA CLP) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or polyethylene container 14 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Cyanides, inorganic, solid, n.o.s, 6.1, Poison, UN1588 SAM Companion - Sample Collection Information Document A1-21 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Cyanogen chloride TO-15 (EPA ORD) [Tier III] Air 1-L or 6-L canister (depending on suspected concentration)'6' Specially prepared stainless steel canister For subatmospheric pressure sampling, the canister is evacuated to 0.05 mm Hg; for pressurized sampling, the sample is collected using a pump and flow control arrangement to achieve a typical 101 - 202 kPa (15-30 psig) Analyze immediately/ as soon as possible None Ship canister in container provided by laboratory Standard carrier shipping label AND Cyanogen chloride, 2.3, Poisonous gas, UN1589 Encyclopedia of Anal. Chem. (2006) DOI: 10.1002/9780470027 318.a0809 [Tier II] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer screw cap VOA vial with fluoropolymer-lined septum, filled with no headspace 14 days • Add sodium thiosulfate to remove residual chlorine from treated wastewater samples151 • Adjust to pH < 2 with 1:1 HCI • Cool to < 6°C; keep from freezing Wipe outside of container lean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Cyanogen chloride, 2.3, Poisonous gas, UN1589 Encyclopedia of Anal. Chem. (2006) DOI: 10.1002/9780470027 318.a0809 [Tier II] Solid 5-g coring device or amber VOA vial AND 1 - 2 oz. glass jar (if needed to determine percent moisture) Coring device (If coring device is to be cleaned and reused, samples can be transferred to an amber VOA vial.) AND glass jar (if needed to determine percent moisture) 14 days if frozen; 48 hours if not frozen Cool to < 6°C; freeze within 48 hours to < negative (-) 7°C [Coring tools should not be frozen below-20°C.] Wipe outside of coring device or container clean using a damp, then dry cloth. Place the coring device or container in a zipper bag. Pack samples as described in Footnote (4). Standard carrier shipping label AND Cyanogen chloride, 2.3, Poisonous gas, UN1589 NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type Cyclohexyl sarin (GF) TO-17 (EPA ORD) [Tier III] Air 1 -4 L (flow rate = 16-67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch O.D. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated wastewater samples151 • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Solid 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. SAM Companion - Sample Collection Information Document A1-22 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 1,2-Dichloroethane (degradation product of HD) TO-15 (EPA ORD) [Tier I] Air 1-L or 6-L canister (depending on suspected concentration)'6' Specially prepared stainless steel canister For subatmospheric pressure sampling, the canister is evacuated to 0.05 mm Hg; for pressurized sampling, the sample is collected using a pump and flow control arrangement to achieve a typical 101 - 202 kPa (15-30 psig) 30 days None Ship canister in container provided by laboratory Standard carrier shipping label AND Ethylene dichloride, 3, Flammable, 6.1, Poison, UN 1184 5030C / 8260D (EPA SW-846) [Tier I] Non-Drinking Water 40 ml. Screw cap VOA vial with fluoropolymer-lined septum, filled with no headspace 14 days with acidification; 7 days if not acidified • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Adjust to pH < 2 with 1:1 HCI • Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethylene dichloride, 3, Flammable, 6.1, Poison, UN 1184 524.2/524.3/524.4 (EPA OW) [Tier I] Drinking Water 40 ml. Amber glass or fluoropolymer screw cap VOA vial with fluoro polymer-lined septum, filled with no headspace 14 days with acidification; 24 hours if not acidified Prior to collection of treated water samples, add 25 mg ascorbic acid to container for removal of residual chlorine Method 524.2: Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethylene dichloride, 3, Flammable, 6.1, Poison, UN 1184 • Adjust to pH < 2 with 1:1 HCI Methods 524.3 and 524.4: • Adjust to pH < 2 with maleic acid For all samples: • If residual chlorine is > 5 mg/L, add an additional 25 mg ascorbic acid per each 5 mg/L chlorine • Cool to < 6°C; keep from freezing 5035A / 8260D (EPA SW-846) [Tier I] Solid 5-g coring device or amber VOA vial AND 1 - 2 oz. glass jar (if needed to determine percent moisture) Coring device (If coring device is to be cleaned and reused, samples can be transferred to an amber VOA vial.) AND glass jar (if needed to determine percent moisture) 14 days if frozen; 48 hours if not frozen Cool to < 6°C; freeze within 48 hours to < negative (-) 7°C [Coring tools should not be frozen below-20°C.] Wipe outside of coring device or container clean using a damp, then dry cloth. Place the coring device or container in a zipper bag. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethylene dichloride, 3, Flammable, 6.1, Poison, UN 1184 NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-23 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Dichlorvos TO-10A (EPA ORD) [Tier I] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3535A / 8270E (EPA SW-846) [Tier I] Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 525.2 / 525.3 (EPA OW) [Tier I] Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days Method 525.2: • Add sodium sulfite to empty sample container to remove residual chlorine from treated water samples • Acidify to pH < 2 with 1:1 HCI(a • Cool to < 6°C; keep from freezing Method 525.3 Cto emotv sample container): • Add 4 mg ascorbic acid to remove residual chlorine from treated water samples • Add 14 mg trisodium EDTA, and 400 mg potassium dihydrogen citrate • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Dicrotophos TO-10A (EPA ORD) [Tier I] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3535A / 8270E (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 SAM Companion - Sample Collection Information Document A1-24 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Diesel Range Organics NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3520C / 3535A / 8015D (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Diesel fuel, 3, flammable, UN 1203 3541 / 3545A / 8015D (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Diesel fuel, 3, flammable, UN 1203 3570 / 8290A Appendix A / 8015D (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Diesel fuel, 3, flammable, UN 1203 Diisopropyl methyl- phosphonate (DIMP) (degradation product of GB) TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label D7597-16 (ASTM) [Tier II] Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid 24 hours Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label 538 (EPA OW) [Tier I] Drinking Water 40 mL Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid 14 days • Prior to sample collection, add 400 |jL of ammonium acetate concentrated stock and 80 |jL of concentrated sodium omadine stock to sample container. [If volumes other than 40-mL are collected, the concentration of ammonium acetate and sodium omadine in the sample should be 1.5 g/L and 64 mg/L, respectively.] • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label E2866-12 (ASTM) [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label EPA/600/R-13/224 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label SAM Companion - Sample Collection Information Document A1-25 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Dimethylphosphoramidic acid (degradation product of GA) TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 D7597-16 (ASTM) [Tier III] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 24 hours Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, liquid, n.o.s, 6.1, Poison, UN3278 E2866-12 (ASTM) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 E PA/60 0/R-13/224 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 Dimethylphosphite TO-10A (EPA ORD) [Tier II] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent Analyze immediately/ as soon as possible Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 SAM Companion - Sample Collection Information Document A1-26 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Diphacinone NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type D7644-16 (ASTM) [Tier II] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Prior to collection of treated water samples, add 10 mg of dry ascorbic acid to sample container for removal of residual chlorine • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the -container with non-reactive tape or film. Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Coumarin derivative pesticides, liquid, toxic, 6.1, Poison, UN3023 3541 /3545A (EPA SW-846) / D7644-16 (ASTM) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Coumarin derivative pesticides, solid, toxic, 6.1, Poison, UN3026 3570/8290A Appendix A (EPA SW-846) / D7644-16 (ASTM) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Coumarin derivative pesticides, solid, toxic, 6.1, Poison, UN3026 Disulfoton 5600 (NIOSH) [Tier I] Air 12-240 L (flow rate = 0.2 to 1 L/min) Filter/solid sorbent tube (OVS- 2 tube: 13 mm quartz filter, XAD-2, [270 mg/140 mg]) 30 days if frozen; 10 days if not frozen Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 525.2 / 525.3 (EPA OW) [Tier II] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid Analyze immediately/ as soon as possible Method 525.2: • Add sodium sulfite to empty sample container to remove residual chlorine from treated water samples • Acidify to pH < 2 with 1:1 HCI(a • Cool to < 6°C; keep from freezing Method 525.3 Cto emotv sample container): • Add 4 mg ascorbic acid to remove residual chlorine from treated water samples • Add 14 mg trisodium EDTA, and 400 mg potassium dihydrogen citrate • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 SAM Companion - Sample Collection Information Document A1-27 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Disulfoton sulfone oxon 5600 (NIOSH) [Tier III] Air 12-240 L (flow rate = 0.2 to 1 L/min) Filter/solid sorbent tube (OVS- 2 tube: 13 mm quartz filter, XAD-2, [270 mg/140 mg]) 30 days if frozen; 10 days if not frozen Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 525.2/525.3 Drinking Water 1 L Amber glass or fluoropolymer Analyze immediately/ as Method 525.2: Wipe outside of container clean using a Standard carrier (EPA OW) [Tier III] Non-Drinking Water container with fluoropolymer- lined septum or lid soon as possible • Add sodium sulfite to empty sample container to remove residual chlorine from treated water samples • Acidify to pH < 2 with 1:1 HCI(a • Cool to < 6°C; keep from freezing Method 525.3 Cto emptv damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 sample container): • Add 4 mg ascorbic acid to remove residual chlorine from treated water samples • Add 14 mg trisodium EDTA, and 400 mg potassium dihydrogen citrate • Cool to < 6°C; keep from freezing EPA/600/R-16/114 (EPA NHSRC) [Tier III] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 SAM Companion - Sample Collection Information Document A1-28 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Disulfoton sulfoxide Disulfoton sulfoxide oxon 5600 (NIOSH) [Tier III] Air 12-240 L (flow rate = 0.2 to 1 L/min) Filter/solid sorbent tube (OVS- 2 tube: 13 mm quartz filter, XAD-2, [270 mg/140 mg]) 30 days if frozen; 10 days if not frozen Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 525.2 / 525.3 (EPA OW) [Tier II] Drinking Water Non-Drinking Water 1 L Amber glass orfluoropolymer container with fluoropolymer- lined septum or lid Analyze immediately/ as soon as possible Method 525.2: • Add sodium sulfite to empty sample container to remove residual chlorine from treated water samples • Acidify to pH < 2 with 1:1 HCI(a • Cool to < 6°C; keep from freezing Method 525.3 Cto emotv sample container): • Add 4 mg ascorbic acid to remove residual chlorine from treated water samples • Add 14 mg trisodium EDTA, and 400 mg potassium dihydrogen citrate • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 1,4-Dithiane (degradation product of HD) NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type. EPA/600/R-16/114 (EPA NHSRC) [Tier II] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days • Cool to < 6°C; keep from freezing For treated water samoles: Preservatives/dechlorinating agents not required, but the analyte is not affected by the following, if added to address other analytes: • Sodium sulfite (5) • 1.6-2.0 mg NH4CI For all other samoles: • Adjust pH < 2 with 1:1 HCI Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label SAM Companion - Sample Collection Information Document A1-29 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 EA2192 [S-2- (diisopropylamino)ethyl methylphosphonothioic acid] (hydrolysis product of VX) TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-15/097 (EPA NHSRC) [Tier II] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. 3541 /3545A (EPA SW-846) / E PA/60 0/R-15/097 (EPA NHSRC) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. 3570 /8290A Appendix A (EPA SW-846) / E PA/60 0/R-15/097 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. Ethyl methylphosphonic acid (EMPA) (degradation product of VX) TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 D7597-16 (ASTM) [Tier III - Drinking Water] [Tier II - Non-Drinking Water] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fiuoro polymer- lined septum or lid 24 hours Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, liquid, n.o.s, 6.1, Poison, UN3278 E2866-12 (ASTM) [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 E PA/60 0/R-13/224 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 SAM Companion - Sample Collection Information Document A1-30 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Ethyldichloroarsine (ED) TO-15 (EPA ORD) [Tier III] Air 1-L or 6-L canister (depending on suspected concentration)'6' Specially prepared stainless steel canister For subatmospheric pressure sampling, the canister is evacuated to 0.05 mm Hg; for pressurized sampling, the sample is collected using a pump and flow control arrangement to achieve a typical 101 - 202 kPa (15-30 psig) Analyze immediately/ as soon as possible None Ship canister in container provided by laboratory Standard carrier shipping label AND Ethyldichloroarsine, 6.1, Poison, UN 1892 3535A / 8270E (EPA SW-846) [Tier III] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid Until holding time data are available, analyze immediately/ as soon as possible • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethyldichloroarsine, 6.1, Poison, UN 1892 3541 /3545A / 8270E (EPA SW-846) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethyldichloroarsine, 6.1, Poison, UN 1892 9102 (NIOSH)/ 8270 E (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethyldichloroarsine, 6.1, Poison, UN 1892 Ethylene oxide TO-15 (EPA ORD) [Tier I] Air 1-L or 6-L canister (depending on suspected concentration)'6' Specially prepared stainless steel canister For subatmospheric pressure sampling, the canister is evacuated to 0.05 mm Hg; for pressurized sampling, the sample is collected using a pump and flow control arrangement to achieve a typical 101 - 202 kPa (15-30 psig) 30 days None Ship canister in container provided by laboratory Standard carrier shipping label AND Ethylene oxide (concentration dependent, refer to 40 CFR 172.101 for more information) 5030C / 8260D (EPA SW-846) [Tier II] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer screw cap VOA vial with fiuoro polymer-lined septum, filled with no headspace 14 days with acidification; 7 days if not acidified • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Adjust to pH < 2 with 1:1 HCI • Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethylene oxide (concentration dependent, refer to 40 CFR 172.101 for more information) 5035A / 8260D (EPA SW-846) [Tier II] Solid 5-g coring device or amber VOA vial AND 1 - 2 oz. glass jar (if needed to determine percent moisture) Coring device (If coring device is to be cleaned and reused, samples can be transferred to an amber VOA vial.) AND glass jar (if needed to determine percent moisture) 14 days if frozen; 48 hours if not frozen Cool to < 6°C; freeze within 48 hours to < negative (-) 7°C [Coring tools should not be frozen below-20°C.] Wipe outside of coring device or container clean using a damp, then dry cloth. Place the coring device or container in a zipper bag. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethylene oxide (concentration dependent, refer to 40 CFR 172.101 for more information) NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-31 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Fenamiphos TO-10A (EPA ORD) [Tier II] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Non-Drinking Water 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum Analyze immediately/ as soon as possible. Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 525.2 / 525.3 (EPA OW) [Tier I] Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid Analyze immediately/ as soon as possible Method 525.2: • Add sodium sulfite to empty sample container to remove residual chlorine from treated water samples • Acidify to pH < 2 with 1:1 HCI(a • Cool to < 6°C; keep from freezing Method 525.3 Cto emotv sample container): • Add 4 mg ascorbic acid to remove residual chlorine from treated water samples • Add 14 mg trisodium EDTA, and 400 mg potassium dihydrogen citrate • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Fentanyl NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3520C / 3535A (EPA SW-846) / J. Chromatogr. A (2011) 1218: 1620- 1649 [Tier III] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label 3541 /3545A (EPA SW-846) / J. Chromatogr. A (2011) 1218: 1620- 1649 [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label 3570 /8290A Appendix A (EPA SW-846) J. Chromatogr. A (2011) 1218: 1620- 1649 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label SAM Companion - Sample Collection Information Document A1-32 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Fluoride NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 300.1, Rev 1.0 (EPA OW) [Tier I] Drinking Water Non-Drinking Water 40 ml. Fluoropolymer, plastic or glass container 28 days Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type Fluoroacetamide J. Chromatogr. B (2008) 876(1): 103-108 [Tier III] Air TBD TBD Until holding time data are available, analyze immediately/ as soon as possible Cool to <6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetamide, 6.1, Poison, UN 1687 J. Chromatogr. B (2008) 876(1): 103-108 [Tier II] Drinking Water Non-Drinking Water 40 ml. Screw-cap VOA vial with fluoropolymer-lined septum Until holding time data are available, analyze immediately/ as soon as possible Cool to <6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetamide, 6.1, Poison, UN 1687 J. Chromatogr. B (2008) 876(1): 103-108 [Tier II] Solid 40 ml. Screw-cap VOA vial with fluoro polymer-lined septum Until holding time data are available, analyze immediately/ as soon as possible Cool to <6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetamide, 6.1, Poison, UN 1687 J. Chromatogr. B (2008) 876(1): 103-108 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Screw-cap VOA vial with fluoro polymer-lined septum Until holding time data are available, analyze immediately/ as soon as possible Cool to <6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetamide, 6.1, Poison, UN 1687 Fluoroacetic acid and fluoroacetate salts (analyze as fluoroacetate ion) S301-1 (NIOSH) J. Chromatogr. A (2007) 1139: 271-278 [Tier III] Air Up to 480 L (at 1.5- 2.0 L/min) Cellulose ester membrane filter/cleaned and rinsed glass or plastic bottles Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetic acid, 6.1, Poison, UN2642 J. Chromatogr. B (2010) 878: 1045-1050 [Tier III] Drinking Water Non-Drinking Water 40 ml. Screw-cap VOA vial with fluoro polymer-lined septum Until holding time data are available, analyze immediately/ as soon as possible Cool to <6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetic acid, 6.1, Poison, UN2642 J. Chromatogr. A (2007) 1139: 271-278 [Tier III] Solid 250 mL Wide-mouth glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetic acid, 6.1, Poison, UN2642 J. Chromatogr. A (2007) 1139: 271-278 [ Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetic acid, 6.1, Poison, UN2642 SAM Companion - Sample Collection Information Document A1-33 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 2-Fluoroethanol 2513 (NIOSH) [Tier III] Air 2 (at 5 ppm) - 35 L (flow rate = 0.01 - 0.2 L/min) Solid sorbenttube (petroleum charcoal [100 mg/50 mg]) 14 days Cool to < 6°C Wipe outside of sorbenttube clean using a damp, then dry cloth. Cap tubes with plastic (not rubber) caps. Place tubes in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label 5030C / 8260D (SW-846) [Tier III] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer screw cap VOA vial with fluoropolymer-lined septum, filled with no headspace 14 days with acidification; 7 days if not acidified • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Adjust to pH < 2 with 1:1 HCI • Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label 5035A / 8260D (EPA SW-846) [Tier III] Solid 5-g coring device or amber VOA vial AND 1 - 2 oz. glass jar (if needed to determine percent moisture) Coring device (If coring device is to be cleaned and reused, samples can be transferred to an amber VOA vial.) AND glass jar (if needed to determine percent moisture) 14 days if frozen; 48 hours if not frozen Cool to < 6°C; freeze within 48 hours to < negative (-) 7°C [Coring tools should not be frozen below-20°C.] Wipe outside of coring device or container clean using a damp, then dry cloth. Place the coring device or container in a zipper bag. Pack samples as described in Footnote (4). Standard carrier shipping label NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type Fluorosilicic acid (analyze as fluoride) NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 300.1, Rev 1.0 (EPA OW) [Tier I] Drinking Water Non-Drinking Water 40 ml. Screw-cap VOA vial with fluoro polymer-lined septum 28 days Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-34 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Formaldehyde 2016 (NIOSH) [Tier I] Air 1 L (at 0.25 mg/m3) - 15 L (at 2.5 mg/m3) (flow rate = 0.03 - 1.5 L/min) Dinitrophenylhydrazine (DNPH)-coated silica gel cartridge 30 days Cool to < 6°C Place end caps onto the cartridge and seal sampler in an envelope. Place cartridge in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Formaldehyde (concentration dependent, refer to 40 CFR 172.101 for more information) 8315A (EPA SW-846) [Tier I] Non-Drinking Water 100 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 3 days • Preserve to pH 5.0 with 1:1 HCI or NaOH • Cool to < 6°C; keep from freezing • Add 10 mg NH4CI to remove residual chlorine from treated water samples Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Formaldehyde (concentration dependent, refer to 40 CFR 172.101 for more information). 556.1 (EPA OW) [Tier I] Drinking Water 30 mL Amber glass or fluoropolymer container with polypropylene screw cap equipped with fluoropolymer-lined septum 7 days • Prior to collecting treated water samples, add 15 mg copper sulfate pentahydrate and 15 mg NH4CI to sample container • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Formaldehyde (concentration dependent, refer to 40 CFR 172.101 for more information) 8315A (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 3 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Formaldehyde (concentration dependent, refer to 40 CFR 172.101 for more information) 3570 / 8290A Appendix A / 8315A (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 3 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Formaldehyde (concentration dependent, refer to 40 CFR 172.101 for more information) Gasoline Range Organics NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 5030C / 8015D (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer screw cap VOA vial with fluoro polymer-lined septum, filled with no headspace 14 days with acidification; 7 days if not acidified • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Adjust to pH < 2 with 1:1 HCI • Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier AND Gasoline identification set, 2.3, Poison gas, NA9035 shipping label 5035A / 8015C (EPA SW-846) [Tier I] Solid 5-g coring device or amber VOA vial AND 1 - 2 oz. glass jar (if needed to determine percent moisture) Coring device (If coring device is to be cleaned and reused, samples can be transferred to an amber VOA vial.) AND glass jar (if needed to determine percent moisture) 14 days if frozen; 48 hours if not frozen Cool to < 6°C; freeze within 48 hours to < negative (-) 7°C [Coring tools should not be frozen below -20°C.] Wipe outside of coring device or container clean using a damp, then dry cloth. Place the coring device or container in a zipper bag. Pack samples as described in Footnote (4). Standard carrier AND Gasoline identification set, 2.3, Poison gas, NA9035 3570 /8290A Appendix A/ 8015D (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier AND Gasoline identification set, 2.3, Poison gas, NA9035 SAM Companion - Sample Collection Information Document A1-35 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Hexahydro-1,3,5-trin itro-1,3,5- triazine (RDX) NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3535A / 8330B (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND RDX, 1.1, Explosive, UN0483® 8330B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND RDX, 1.1, Explosive, UN0483™ 3570 / 8290A Appendix A / 8330B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND RDX, 1.1, Explosive, UN0483™ Hexamethylene triperoxidediamine (HMTD) NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3535A / 8330B (EPA SW-846) / Analyst (2001) 126:1689-1693 [Tier II] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND HMTD, 1.1, Explosive181 8330B (EPA SW-846) / Analyst (2001) 126:1689-1693 [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND HMTD, 1.1, Explosive181 3570 /8290A Appendix A (EPA SW-846) / Analyst (2001) 126:1689-1693 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND HMTD, 1.1 , Explosive'8' Hydrogen bromide 7907 (NIOSH) [Tier I] Air 30-600 L (flow rate = 2 L/min) Two filters in series separated with chemically inert spacer: • pre-filter: 37-mm diameter quartz fiber • sampling filter: 37-mm diameter quartz fiber impregnated with 1 M 500-|jL Na2C03 28 days Cool to < 6°C Wipe outside of filter clean using a damp, then dry cloth. Place filter in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Hydrogen bromide, 2.3, Poisonous gas, UN1048 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-36 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Hydrogen chloride 7907 (NIOSH) [Tier I] Air 30-600 L (flow rate = 2 L/min) Two filters in series separated with chemically inert spacer: • pre-filter: 37-mm diameter quartz fiber • sampling filter: 37-mm diameter quartz fiber impregnated with 1 M 500-|jL Na2C03 28 days Cool to < 6°C Wipe outside of filter clean using a damp, then dry cloth. Place filter in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Hydrogen chloride, 2.3, Poisonous gas, UN1050 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type Hydrogen cyanide 6010 (NIOSH) [Tier I] Air 2 (at 5 ppm) - 90 L (flow rate = 0.05 - 0.2 L/min) Solid sorbenttube (soda lime [600 mg/200 mg]) 14 days Cool to < e°c Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap tubes with plastic (not rubber) caps. Place tubes in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Hydrogen cyanide, 2.3, Poisonous gas, UN1956 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type Hydrogen fluoride 7906 (NIOSH) [Tier I] Air 30 - 600 L (flow rate = 2 L/min) Two filters in series separated by a chemically inert spacer: • pre-filter: cellulose nitrate, 0.8 l_im pore size, 37-mm diameter • treated filter: cellulose nitrate impregnated with 0.75 M Na2C03, 0.8 |jm pore size, 37- mm diameter 28 days Cool to < 6°C Wipe outside of filter clean using a damp, then dry cloth. Place filter in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Hydrogen fluoride, 2.3, Poisonous gas, Corrosive, UN1052 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type Hydrogen sulfide 6013 (NIOSH) [Tier I] Air 1.2 (at 10 ppm)-40 L (flow rate = 0.1 - 1.5 L/min; 0.2 L/min is recommended) Filter/solid sorbent tube (0.5 pm/coconut shell charcoal [400 mg/200 mg]) 21 days Cool to < 6°C Wipe filter and sorbenttube clean, using a damp, then dry cloth. Place filter and sorbenttube in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Hydrogen sulfide, 2.3, Poisonous gas, Corrosive, UN 1053 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-37 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Isopropyl methylphosphonic acid (IMPA) (degradation product of GB) TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 D7597-16 (ASTM) [Tier III - Drinking Water] [Tier II - Non-Drinking Water] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 24 hours Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, liquid, n.o.s, 6.1, Poison, UN3278 E2866-12 (ASTM) [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 EPA/600/R-13/224 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 Kerosene NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3520C / 3535A / 8015D (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fiuoro polymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Kerosene, 3, Flammable, UN 1223 3541 / 3545A / 8015D (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Kerosene, 3, Flammable, UN 1223 3570 / 8290A Appendix A / 8015D (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth glass or fluoropolymer container with fiuoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Kerosene, 3, Flammable, UN 1223 SAM Companion - Sample Collection Information Document A1-38 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container*2' Holding Time Preservation Packaging Requirements Shipping Label131 Lead arsenate (analyze as total arsenic) 10-3.1 / 10-3.4/ 10-3.5 (EPA ORD) [Tier 1] Air Up to 1700 m3 Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Lead arsenate, 6.1, Poison, UN1617 3015A / 6010D / 6020B (EPA SW-846) [Tier I] Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Lead arsenate, 6.1, Poison, UN1617 200.7/200.8 (EPA OW) [Tier I] Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Lead arsenate, 6.1, Poison, UN1617 3050B / 3051A / 6010D / 6020B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Lead arsenate, 6.1, Poison, UN1617 9102 (NIOSH) 6010D / 6020B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Lead arsenate, 6.1, Poison, UN1617 Lewisite 1 (L-1) Lewisite 2 (L-2) Lewisite 3 (L-3) IO-3.1 IO-3.4/IO-3.5 (EPA ORD) [Tier I] Air Up to 1700 m3 Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. E PA/60 O/R-15/258 (EPA NHSRC) [Tier II - Lewisite I] [Tier III - Lewisites II and III] Drinking Water Non-Drinking Water 40 mL VOA vial with fluoropolymer- lined cap 14 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-15/258 (EPA NHSRC) [Tier II - Lewisite I] [Tier III - Lewisites II and III] Solid 40 mL VOA vial with fluoropolymer- lined cap Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labelinq. EPA/60 O/R-15/258 (EPA NHSRC) [Tier II - Lewisite I] [Tier III - Lewisites II and III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 VOA vial with fluoropolymer- lined cap 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labelina. SAM Companion - Sample Collection Information Document A1-39 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Lewisite oxide (degradation product of Lewisite) (analyze as total arsenic) 10-3.1 / 10-3.4/ 10-3.5 (EPA ORD) [Tier 1] Air Up to 1700 m" Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-15/258 (EPA NHSRC) [Tier III] Drinking Water Non-Drinking Water 40 mL Screw-cap VOA vial with fluoropolymer-lined septum 14 days • Acidify to pH < 2 with HN03 • Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-15/258 (EPA NHSRC) [Tier III] Solid 40 mL Screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-15/258 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. Mercuric chloride (analyze as total mercury) NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 245.1 (EPA OW) [Tier I] Drinking Water Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 28 days Acidify to pH < 2 with HN03(71 Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Mercuric chloride, 6.1, Poison, UN 1624 7473 / 7471B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container 28 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Mercuric chloride, 6.1, Poison, UN 1624 9102 (NIOSH)/ 7473 / 7471B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container 28 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Mercuric chloride, 6.1, Poison, UN 1624 SAM Companion - Sample Collection Information Document A1-40 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Mercury, Total IO-5 (EPA ORD) [Tier I] Air Typical flow rate is 30 L/minfor 12-24 hours Trap apparatus: • Vapor phase: Sampling pumps specially designed for trace-level pollutant sampling; vapor phase Hg collected using two gold traps in series, attached to a fluoropolymer filter pack • Particle phase: Glass fiber filter 7 days [Particle-phase Hg filters can be stored indefinitely at negative (-) 40°C.] Cool to < 6°C Wipe the outside of apparatus clean, cap both ends. Place in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Mercury, 8, Corrosive, UN2809 245.1 (EPA OW) [Tier I] Drinking Water Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 28 days Acidify to pH < 2 with HN03(71 [If dissolved mercury is to be determined, filter the sample prior to acidification.] Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Mercury, 8, Corrosive, UN2809 7473 / 7471B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container 28 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Mercury, 8, Corrosive, UN2809 9102 (NIOSH)/ 7473 / 7471B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container 28 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Mercury, 8, Corrosive, UN2809 SAM Companion - Sample Collection Information Document A1-41 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container*2' Holding Time Preservation Packaging Requirements Shipping Label131 Methamidophos J. Chromatogr. A (2007) 1154(1): 3-25 [Tier III] Air TBD TBD Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Chromatographia (2006) 63(5/6): 223-237 [Tier II] Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 538 (EPA OW) [Tier I] Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Prior to collection, add 400 pL of ammonium acetate concentrated stock and 80 pL of concentrated sodium omadine stock to sample container. [If volumes other than 40-mL are collected, the concentration of ammonium acetate and sodium omadine in the sample should be 1.5 g/L and 64 mg/L, respectively.] • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 J. Chromatogr. A (2007) 1154(1): 3-25 [Tier II] Solid 250 mL Fluoropolymer centrifuge tube Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 J. Chromatogr. A (2007) 1154(1): 3-25 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth glass or fluoropolymer container with fluoropolymer-lined lid Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 SAM Companion - Sample Collection Information Document A1-42 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Methomyl 5601 (NIOSH) [Tier I] Air 12-480 L (flow rate = 0.1 - 1 L/min) Filter/solid sorbenttube: • 13- mm quartz fiber filter • OVS-2 tube (XAD-2 resin [270mg/140 mg]) 30 days if frozen; 7 days at room temperature (24°C) Cool to < 6°C Wipe outside of sorbenttube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 D7645-16 (ASTM) [Tier II] Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Acidify to pH < 3.8 with glacial acetic acid • Cool to < 6°C; keep from freezing • Prior to collection of treated water samples, add 10 mg ascorbic acid to sample container Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 531.2 (EPA OW) [Tier I] Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 28 days • Prior to collection, add 0.4 g potassium dihydrogen citrate and 3.2 - 4.8 mg sodium thiosulfate to sample container • Agitate for 1 minute after collection • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 8318A (EPA SW-846) [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 3570/8290A Appendix A/ 8318A (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 Methoxyethylmercuric acetate (analyze as total mercury) IO-5 (EPA ORD) [Tier I] Air Typical flow rate of 30 L/min for 12-24 hours Trap apparatus: • Vapor phase: Sampling pumps specially designed for trace-level pollutant sampling; vapor phase Hg collected using two gold traps in series, attached to a fluoropolymer filter pack • Particle phase: Glass fiber filter 7 days [Particle-phase Hg filters can be stored indefinitely at negative (-) 40°C.] Cool to < 6°C Wipe the outside of trap apparatus clean, cap both ends. Place in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Mercury compounds, solid, n.o.s, 6.1, Poison, UN2025 245.1 (EPA OW) [Tier I] Drinking Water Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 28 days Acidify to pH < 2 with HN03(71 Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Mercury compounds, liquid, n.o.s, 6.1, Poison, UN2024 7473 / 7471B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container 28 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Mercury compounds, solid, n.o.s, 6.1, Poison, UN2025 9102 (NIOSH)/ 7473 / 7471B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container 28 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Mercury compounds, solid, n.o.s, 6.1, Poison, UN2025 SAM Companion - Sample Collection Information Document A1-43 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Methyl acrylonitrile PV2004 (OSHA) [Tier III] Air 120 L (flow rate = 1 L/min) Filter/solid sorbenttube (13- mm quartz fiber filter) • OVS-7 tube (XAD-7 resin) • OVS-2 (XAD-2 resin) • OVS-SG (silica gel) 13 days Cool to < 6°C Wipe outside of sorbenttube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Methyl acrylonitrile, 3.0, Flammable Liquid, 6.1, Poison, UN3079 524.2/524.3/524.4 (EPA OW) [Tier II] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer screw cap VOA vial with fluoropolymer-lined septum, filled with no headspace 14 days with acidification; 24 hours if not acidified Prior to collection of treated water samples, add 25 mg ascorbic acid to container for removal of residual chlorine Method 524.2: Wipe outside of container clean using a damp, then dry cloth. Seal the-container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Methyl acrylonitrile, 3.0, Flammable Liquid, 6.1, Poison, UN3079 • Adjust to pH < 2 with 1:1 HCI Methods 524.3 and 524.4: • Adjust to pH < 2 with maleic acid For all samples: • If residual chlorine is > 5 mg/L, add an additional 25 mg ascorbic acid per each 5 mg/L chlorine • Cool to < 6°C; keep from freezing 5035A / 8260D (EPA SW-846) [Tier II] Solid 5-g coring device or amber VOA vial AND 1 - 2 oz. glass jar (if needed to determine percent moisture) Coring device (If coring device is to be cleaned and reused, samples can be transferred to an amber VOA vial.) AND glass jar (if needed to determine percent moisture) 14 days if frozen; 48 hours if not frozen Cool to < 6°C; freeze within 48 hours to < negative (-) 7°C. [Coring tools should not be frozen below-20°C.] Wipe outside of coring device or container clean using a damp, then dry cloth. Place the coring device or container in a zipper bag. Pack samples as described in Footnote (4). Standard carrier shipping label AND Methyl acrylonitrile, 3.0, Flammable Liquid, 6.1, Poison, UN3079 3570 /8290A Appendix A/ 8260D (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Screw-cap VOA vial with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Methyl acrylonitrile, 3.0, Flammable Liquid, 6.1, Poison, UN3079 3-Methyl fentanyl NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3520C / 3535A (EPA SW-846) / J. Chromatogr. B (2014) 962: 52-58 [Tier III] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organic, n.o.s., liquid, 6.1, Poison, UN2810 3541 /3545A (EPA SW-846) / J. Chromatogr. B (2014) 962: 52-58 [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organic, n.o.s., solid, 6.1, Poison, UN2811 3570 /8290A (EPA SW-846) / J. Chromatogr. B (2014) 962: 52-58 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organic, n.o.s., solid, 6.1, Poison, UN2811 SAM Companion - Sample Collection Information Document A1-44 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Methyl fluoroacetate (analyze as fluoroacetate ion) S301-1 (NIOSH) J. Chromatogr. A (2007) 1139: 271-278 [Tier III] Air Up to 480 L (flow rate = 1.5-2.0 L/min) Cellulose ester membrane filter/cleaned and rinsed glass or plastic bottles Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetic acid, 6.1, Poison, UN2642 J. Chromatogr. B (2010) 878: 1045-1050 [Tier III] Drinking Water Non-Drinking Water 40 mL Screw-cap VOA vial with fluoropolymer-lined septum Until holding time data are available, analyze immediately/ as soon as possible Cool to <6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetic acid, 6.1, Poison, UN2642 J. Chromatogr. A (2007) 1139: 271-278 [Tier III] Solid 250 mL Wide-mouth glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetic acid, 6.1, Poison, UN2642 J. Chromatogr. A (2007) 1139: 271-278 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Fluoroacetic acid, 6.1, Poison, UN2642 Methyl hydrazine 3510 (NIOSH) [Tier I] Air 3 (at 0.2 ppm) - 20 L (flow rate = 0.5 to 1.5 L/min) Bubbler (0.1 M HCI). Seal and transfer the bubbler to container for shipping. 5 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Methyl hydrazine, 6.1, Poison, UN 1244 J. Chromatogr. (1993) 617: 157-162 [Tier II] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Methyl hydrazine, 6.1, Poison, UN 1244 3541 /3545A (EPA SW-846) J. Chromatogr. (1993) 617: 157-162 [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Methyl hydrazine, 6.1, Poison, UN 1244 3570 /8290A Appendix A (EPA SW-846) / J. Chromatogr. (1993)617: 157-162 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Methyl hydrazine, 6.1, Poison, UN 1244 Methyl isocyanate OSHA54 [Tier I] Air 15 L (flow rate = 0.05 L/min) Solid sorbenttube (XAD-7 tubes coated with 0.03 mg of 1- (2- py ri dy I) pi pe raz i ne 18 days • Cool to < 6°C • Protect from light Wipe outside of tube clean using a damp, then dry cloth. Cap ends of tube, wrap tube with OSHA seal, and place in container. Seal the container with non-reactive tape or film. Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Methyl isocyanate, 6.1, Poison, UN2480 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-45 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Methyl paraoxon TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3535A / 8270E (EPA SW-846) [Tier III] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Methyl parathion TO-10A (EPA ORD) [Tier I] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3535A / 8270E (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Methylamine OSHA40 [Tier I] Air 10 L (flow rate = 0.2 L/min) Solid sorbent tubes containing XAD-7 resin coated with 10% NBD chloride (7-chloro-4- nitrobenzo-2-oxa-1,3-diazole) by weight 15 days Cool to < 6°C Wipe outside of tube clean using a damp, then dry cloth. Cap ends of tube, wrap tube with OSHA seal, and place in container. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND, Methylamine, anhydrous, 2.1, Flammable gas, UN1061 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-46 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Methylphosphonic acid (MPA) (degradation product of VX, GB, &GD) TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 D7597-16 (ASTM) [Tier III - Drinking Water] [Tier II - Non-Drinking Water] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 24 hours Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, liquid, n.o.s, 6.1, Poison, UN3278 E2866-12 (ASTM) [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 E PA/60 0/R-13/224 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 SAM Companion - Sample Collection Information Document A1-47 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Mevinphos TO-10A (EPA ORD) [Tier II] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3535A / 8270E (EPA SW-846) [Tier I] Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 525.2 / 525.3 (EPA OW) [Tier I] Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid Analyze immediately/ as soon as possible Method 525.2: • Add sodium sulfite to empty sample container to remove residual chlorine from treated water samples • Acidify to pH < 2 with 1:1 HCI(a • Cool to < 6°C; keep from freezing Method 525.3 Cto emotv sample container): • Add 4 mg ascorbic acid to remove residual chlorine from treated water samples • Add 14 mg trisodium EDTA, and 400 mg potassium dihydrogen citrate • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 SAM Companion - Sample Collection Information Document A1-48 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Monocrotophos TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3535A / 8270E (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 3541 / 3545A / 8270E (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3570 / 8290 Appendix A/ 8270 E (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 14 days Cool 2 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Mustard, nitrogen (HN-1) Mustard, nitrogen (HN-2) Mustard, nitrogen (HN-3) TO-17 (EPA ORD) [Tier III] Air 1 - 4 L (flow rate = 16-67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-12/653 (EPA NHSRC) [Tier II- HN-1 and HN-3] [Tier III - HN-2] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fiuoro polymer- lined septum or lid 7 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. E PA/60 0/R-12/653 (EPA NHSRC) [Tier II- HN-1 and HN-3] [Tier III - HN-2] Solid 40 mL Amber glass or fluoropolymer container with fiuoro polymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-12/653 (EPA NHSRC) [Tier II- HN-1 and HN-3] [Tier III - HN-2] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. SAM Companion - Sample Collection Information Document A1-49 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Mustard, sulfur (HD) TO-17 (EPA ORD) [Tier III] Air 1 - 4 L (flow rate = 16 - 67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid Analyze immediately/ as soon as possible Cool to < 6°C; keep from freezing • Add sodium thiosulfate to remove residual chlorine from treated water samples15' Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Solid 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. N-Ethyldiethanolamine (EDEA) (degradation product of HN-1) 3509 (NIOSH) [Tier III] Air 0.5 - 300 L (flow rate = 0.5-1 L/min) Impinger (15 mL 2 mM hexanesulfonic acid). [Impinger contents are transferred into VOA vial for shipping.] 21 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 D7599-16 (ASTM) [Tier II Non-Drinking Water] [Tier III Drinking Water] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 3541 /3545A (EPA SW-846) / E PA/60 0/R-11/143 (EPA NHSRC/CDC) [SAM Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 EPA/600/R-11/123 (EPA NHSRC/CDC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 125 mL polypropylene container with a polypropylene screw cap 28 days • Cool to < 6°C • Protect from light Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 SAM Companion - Sample Collection Information Document A1-50 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Nicotine compounds (analyze as nicotine) NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3535A / 8270E (EPA SW-846) [Tier II] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Nicotine compounds, liquid, n.o.s, 6.1, Poison, UN3144 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Nicotine compounds, solid, n.o.s, 6.1, Poison, UN1655 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Nicotine compounds, solid, n.o.s, 6.1, Poison, UN1655 N-Methyl diethanolamine (MDEA) (degradation product of HN-2) 3509 (NIOSH) [Tier III] Air 0.5 - 300 L (flow rate = 0.5-1 L/min) Impinger (15 mL 2 mM hexanesulfonic acid) [Impinger contents are transferred into VOA vial for shipping.] 21 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 D7599-16 (ASTM) [Tier III - Drinking Water] [Tier II - Non-Drinking Water] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid 7 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 3541 /3545A (EPA SW-846) / EPA/600/R-11/123 (EPA NHSRC/CDC) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 EPA/600/R-11/123 (EPA NHSRC/CDC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 125 mL polypropylene container with a polypropylene screw cap 28 days • Cool to < 6°C • Protect from light Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 SAM Companion - Sample Collection Information Document A1-51 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 1-Methylethyl ester ethylphosphonofluoridic acid (GE) TO-17 (EPA ORD) [Tier III] Air 1 - 4 L (flow rate = 16 - 67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier III] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Cool to < 6°C; keep from freezing • Add sodium thiosulfate to remove residual chlorine from treated water samples15' Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier III] Solid 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. Octahydro-1,3,5,7-tetranitro- 1,3,5,7-tetrazocine (HMX) NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3535A / 8330B (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND HMX, 1.1, Explosive, UN0391™ 8330B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND HMX, 1.1 Explosive, UN0391™ 3570 / 8290 Appendix A/ 8330B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND HMX, 1.1, Explosive, UN0391™ SAM Companion - Sample Collection Information Document A1-52 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Osmium tetraoxide (analyze for total osmium) 10-3.1 / IO-3.4 (EPA ORD) [Tier II] Air Up to 1700 m3 Filter (quartz, silica or cellulose fiber) Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Osmium tetroxide, 6.1, Poison, UN2471 3015A / 6010D / 6020B (EPA SW-846) [Tier II] Drinking Water Non-Drinking Water 125 mL Glass container 6 months with acidification. Samples must be acidified within 14 days of collection. 1% nh4oh 171 Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Osmium tetroxide, 6.1, Poison, UN2471 3051A / 6010D / 6020B (EPA SW-846) [Tier II] Solid 250 mL Wide-mouth fluoropolymer or glass container with fluoropolymer-lined lid Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Osmium tetroxide, 6.1, Poison, UN2471 3051A / 6010D / 6020B (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer or glass container with fluoropolymer-lined lid Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Osmium tetroxide, 6.1, Poison, UN2471 Oxamyl 5601 (NIOSH) [Tier I] Air 240 - 480 L (flow rate = 0.1 - 1 L/min) Filter/solid sorbent tube (OVS- 2 tube: 13 mm quartz fiber filter, XAD-2 [270mg/140 mg]) 30 days if frozen; 7 days at room temperature (24°C) Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 D7645-16 (ASTM) [Tier II] Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Acidify to pH < 3.8 with glacial acetic acid • Cool to < 6°C; keep from freezing • Prior to collection of treated water samples, add 10 mg ascorbic acid to sample container Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 531.2 (EPA OW) [Tier I] Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 28 days • Prior to collection, add 0.4 g potassium dihydrogen citrate and 3.2 - 4.8 mg sodium thiosulfate to sample container • Agitate for 1 minute after collection • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 8318A (EPA SW-846) [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 3570 / 8290 Appendix A / 8318A (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 SAM Companion - Sample Collection Information Document A1-53 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Paraoxon TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3520C/3535A / 8270E (EPA SW-846) [Tier III] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Paraquat NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 549.2 (EPA OW) [Tier I] Drinking Water Non-Drinking Water 250 mL High density PVC or silanized amber glass bottle fitted with screw cap 7 days • Add 100 mg/L sodium thiosulfate to remove residual chlorine from treated water samples • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Bipyridilium pesticides, liquid, 6.1, Toxic, UN3016 J. Chromatogr. A (2008) 1196- 1197:110-116 [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Bipyridilium pesticides, Solid, 6.1, Toxic, UN2811 J. Chromatogr. A (2008) 1196- 1197:110-116 [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Bipyridilium pesticides, Solid, 6.1, Toxic, UN2811 SAM Companion - Sample Collection Information Document A1-54 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Parathion TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3520C / 3535A / 8270E (SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Pentaerythritol tetranitrate (PETN) NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3535A / 8330B (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND PETN, 1.1, Explosive, UN0411|::| 8330B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND PETN, 1.1 Explosive, UN0411|::| 3570 / 8290A Appendix A / 8330B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND PETN, 1.1 , Explosive, UN0411|::| SAM Companion - Sample Collection Information Document A1-55 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Phencyclidine TO-10A (EPA ORD) [Tier II] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label EPA/600/R-16/114 (EPA NHSRC) [Tier II] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days • Cool to < 6°C; keep from freezing For treated water samoles: Preservatives/dechlorinating agents not required, but the analyte is not affected by the following, if added to address other analytes: • Sodium sulfite (5) • 1.6-2.0 mg NH4CI For all other samoles: • Can be adjusted to pH < 2 with 1:1 HCI, but this is not required Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label 9106/9109 [NIOSH] (Tier II) Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 30 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label Phorate TO-10A (EPA ORD) [Tier II] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3535A / 8270E (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoro polymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 SAM Companion - Sample Collection Information Document A1-56 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Phorate sulfone Phorate sulfone oxon TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 540 (EPA OW) [Tier I - Phorate sulfone] [Tier III - Phorate sulfone oxon] Drinking Water Non-Drinking Water 250 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 28 days Add the followina to samole containers oriorto collection: • 100 mg/L solid ascorbic acid (for removal of residual chlorine from treated water samples) • 2 g/L 2-chloroacetamide (antimicrobial preservative) • 7.75 g/L Trizma® base (buffering agent) After collectina the samole: • Cap the container and agitate until preservative is dissolved • Cool to <10°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fiuoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fiuoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 SAM Companion - Sample Collection Information Document A1-57 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Phorate sulfoxide Phorate sulfoxide oxon TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 540 (EPA OW) [Tier I - Phorate sulfoxide] [Tier III - Phorate sulfoxide oxon] Drinking Water Non-Drinking Water 250 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 28 days Add the followina to samole containers oriorto collection: • 100 mg/L solid ascorbic acid (for removal of residual chlorine from treated water samples) • 2 g/L 2-chloroacetamide (antimicrobial preservative) • 7.75 g/L Trizma® base (buffering agent) After collectina the samole: • Cap the container and agitate until preservative is dissolved • Cool to <10°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Phosgene OSHA61 [Tier I] Air 240 L (flow rate = 1 L/min) Si lane-treated glass tubes [4- mm i.d. * 6-mm o.d. * 11-cm long, and packed with 150 mg/75 mg of pre treated XAD-2 adsorbent coated with 2-HMP (2-[hydroxymethyl] piperidine)] 19 days Cool to < 6°C Wipe outside of tube clean using a damp, then dry cloth. Cap ends of tube, wrap tube with OSHA seal, and place in an air tight container containing activated carbon. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Phosgene, 2.3, Poisonous gas, UN1076 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-58 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Phosphamidon TO-10A (EPA ORD) [Tier II] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3520C / 3535A / 8270E (EPA SW-846) [Tier I] Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 525.3 (EPA OW) [Tier I] Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days Method 525.2: • Add sodium sulfite to empty sample container to remove residual chlorine from treated water samples • Acidify to pH < 2 with 1:1 HCI(a • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 Method 525.3 Cto emotv sample container): • Add 4 mg ascorbic acid to remove residual chlorine from treated water samples • Add 14 mg trisodium EDTA, and 400 mg potassium dihydrogen citrate • Cool to < 6°C; keep from freezing EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 Phosphine 6002 (NIOSH) [Tier I] Air 1 (at 0.3 ppm) - 16 L (flow rate = 0.01 - 0.2 L/min) Sorbent tube (Hg(CN)2 coated silica gel [300 mg/150 mg]) 7 days Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap tubes with plastic (not rubber) caps. Place tubes in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Phosphine, 2.3, Poisonous gas, UN2199 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-59 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container*2' Holding Time Preservation Packaging Requirements Shipping Label131 Phosphorus trichloride 6402 (NIOSH) [Tier I] Air 11 (at 0.5 ppm)- 100 L (flow rate = 0.05-0.2 L/min) Bubbler (15 mL H20) Seal and transfer the bubbler to container for shipping. Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C; keep from freezing Wipe outside of ontainer clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap each glass container with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Phosphorus trichloride, 6.1, Poison, UN1809 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Solid For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type Pinacolyl methyl phosphonic acid (PMPA) (degradation product of GD) TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 D7597-16 (ASTM) [Tier III - Drinking Water] [Tier II - Non-Drinking Water] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 24 hours Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, liquid, n.o.s, 6.1, Poison, UN3278 E2866-12 (ASTM) [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 EPA/600/R-13/224 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth glass or fluoropolymer container with fiuoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus compound, toxic, solid, n.o.s, 6.1, Poison, UN3464 Propylene oxide 1612 (NIOSH) [Tier I] Air 0.5 (at 100 ppm) - 5 L (flow rate = 0.01 - 0.2 L/min) Solid sorbent tube (coconut shell charcoal [100 mg/50 mg]) 6 weeks if frozen; 7 days if not frozen Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap tubes with plastic (not rubber) caps. Place tubes in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Propylene oxide, 3, Flammable, UN1280. 5030C / 8260D (EPA SW-846) [Tier II] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer screw cap VOA vial with fiuoro polymer-lined septum, filled with no headspace 14 days with acidification; 7 days if not acidified • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Adjust to pH < 2 with 1:1 HCI • Cool to < 6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Propylene oxide, 3, Flammable liquid, UN1280 5035A / 8260D (EPA SW-846) [Tier II] Solid 5-g coring device or amber VOA vial AND 1 - 2 oz. glass jar (if needed to determine percent moisture) Coring device (If coring device is to be cleaned and reused, samples can be transferred to an amber VOA vial.) AND glass jar (if needed to determine percent moisture) 14 days if frozen; 48 hours if not frozen Cool to < 6°C; freeze within 48 hours to < negative (-) 7°C [Coring tools should not be frozen below-20°C.] Wipe outside of coring device or container clean using a damp, then dry cloth. Place the coring device or container in a zipper bag. Pack samples as described in Footnote (4). Standard carrier shipping label AND Propylene oxide, 3, Flammable, UN 1280 NA Wipes For the purposes of this document, this analyte is not considered to be a concern in this sample type SAM Companion - Sample Collection Information Document A1-60 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 R-33 (VR) [methylphosphonothioic acid, S-[2-(diethyIamino)ethyl]0-2- m ethyl propyl ester] TO-17 (EPA ORD) [Tier III] Air 1 - 4 L (flow rate = 16 - 67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-12/653 (EPA NHSRC) [Tier II] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Cool to < 6°C; keep from freezing • Add sodium thiosulfate to remove residual chlorine from treated water samples15' Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-12/653 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-12/653 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. Sarin (GB) TO-17 (EPA ORD) [Tier III] Air 1 - 4 L (flow rate = 16-67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Cool to < 6°C; keep from freezing • Add sodium thiosulfate to remove residual chlorine from treated water samples15' Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Solid 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. SAM Companion - Sample Collection Information Document A1-61 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container*2' Holding Time Preservation Packaging Requirements Shipping Label131 Sodium arsenite (analyze as total arsenic) 10-3.1 / 10-3.4/ 10-3.5 (EPA ORD) [Tier 1] Air Up to 1700 m3 Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Sodium arsenite, solid, 6.1, Poison, UN2027 3015A / 6010D / 6020B (EPA SW-846) [Tier I] Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Sodium arsenite, aqueous solutions, 6.1, Poison, UN 1686 200.7 / 200.8 (EPA OW) [Tier I] Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) [If arsenic species are to be determined, remove unpreserved aliquot prior to sample preservation.] • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Sodium arsenite, aqueous solutions, 6.1, Poison, UN 1686 3050B / 3051A / 6010D / 6020B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Sodium arsenite, solid, 6.1, Poison, UN2027 9102 (NIOSH)/ 6010D / 6020B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Sodium arsenite, solid, 6.1, Poison, UN2027 Sodium azide (analyze as azide ion) ID-211 (OSHA) [Tier I] Air 5 L (flow rate = 1 L/min) Filter / Sorbent tube (PVC or quartz filter with silica coated sorbent [150 mg/75 mg]) 10 days Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Sodium azide, 6.1, Poison, UN 1687 J. of Forensic Sciences (1998) 43(1): 200-202/ 300.1, Rev 1.0 (EPA OW) [Tier II] Drinking Water Non-Drinking Water 40 mL Screw-cap VOA vial with fluoropolymer-lined septum Until holding time data are available, analyze immediately/ as soon as possible Cool to <6°C; keep from freezing Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Sodium azide, 6.1, Poison, UN 1687 J. of Forensic Sciences (1998) 43(1): 200-202/ 300.1, Rev 1.0 (EPA OW) [Tier II] Solid 250 mL Wide-mouth glass or fluoropolymer container with fluoro polymer-lined septum or lid Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Sodium azide, 6.1, Poison, UN 1687 ID-211 (OSHA) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth glass or fluoropolymer container with fluoro polymer-lined septum or lid 10 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Sodium azide, 6.1, Poison, UN 1687 SAM Companion - Sample Collection Information Document A1-62 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Soman (GD) TO-17 (EPA ORD) [Tier III] Air 1 - 4 L (flow rate = 16 - 67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Cool to < 6°C; keep from freezing • Add sodium thiosulfate to remove residual chlorine from treated water samples15' Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Solid 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/115 (EPA NHSRC) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. Strychnine NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3535A / 8270E (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Strychnine, 6.1, Poison, UN1692 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Strychnine, 6.1, Poison, UN1692 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Strychnine, 6.1, Poison, UN1692 SAM Companion - Sample Collection Information Document A1-63 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 Tabun (GA) TO-17 (EPA ORD) [Tier III] Air 1 - 4 L (flow rate = 16 - 67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-12/653 (EPA NHSRC) [Tier II] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-12/653 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-12/653 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap containers with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. Tetraethyl pyrophosphate (TEPP) TO-10A (EPA ORD) [Tier II] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 3511 / 8270E (EPA SW-846) [Tier II] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, liquid, 6.1, Poison, UN3018 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 ml. Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool samples to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fiuoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool samples to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fiuoropolymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Organophosphorus pesticides, solid, 6.1, Poison, UN2783 SAM Companion - Sample Collection Information Document A1-64 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container*2' Holding Time Preservation Packaging Requirements Shipping Label131 Tetramethylenedisulfo- tetramine (TETS) TO-10A (EPA ORD) [Tier II] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Pesticides, solid, toxic, n.o.s, 6.1, Poison, UN2588 EPA/600/R-16/114 (EPA NHSRC) [Tier I] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days • Cool to < 6°C; keep from freezing For treated water samoles: Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack Standard carrier shipping label AND Pesticides, liquid, toxic, n.o.s, 6.1, Poison, Preservatives/dechlorinating agents not required, but the analyte is not affected by the following, if added to address other analytes: • Sodium sulfite (5) • 1.6-2.0 mg NH4CI For all other samples: samples as described in Footnote (4). UN2902 • Can be adjusted to pH < 2 with 1:1 HCI, but this is not required EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Pesticides, solid, toxic, n.o.s, 6.1, Poison, UN2588 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Pesticides, solid, toxic, n.o.s, 6.1, Poison, UN2588 Thallium sulfate (analyze as total thallium) 10-3.1 / 10-3.4/ 10-3.5 (EPA ORD) [ Tier 1] Air Up to 1700 m3 Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Thallium compounds n.o.s, 6.1, Poison, UN1707 3015A / 6010D / 6020B (EPA SW-846) [Tier I] Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Thallium compounds n.o.s, 6.1, Poison, UN1707 200.7 / 200.8 (EPA OW) [Tier I] Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Thallium compounds n.o.s, 6.1, Poison, UN1707 3050B / 3051A / 6010D / 6020B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Thallium compounds n.o.s, 6.1, Poison, UN1707 9102 (NIOSH)/ 6010D / 6020B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Thallium compounds n.o.s, 6.1, Poison, UN1707 SAM Companion - Sample Collection Information Document A1-65 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Thiodiglycol (TDG) (degradation product of HD) TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Aviation regulated solid, n.o.s, UN3335 D7598-16 (ASTM) [Tier III - Drinking Water] [Tier II - Non-Drinking Water] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 24 hours Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Aviation regulated liquid, n.o.s, UN3334 E2787-11 (ASTM) [Tier II] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fluoropolymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Aviation regulated solid, n.o.s, UN3335 E2838-11 (ASTM) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fluoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Aviation regulated solid, n.o.s, UN3335 SAM Companion - Sample Collection Information Document A1-66 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Thiofanox 5601 (NIOSH) [Tier III] Air 240 - 480 L (flow rate = 0.1-1.0 L/min) Filter/solid sorbent tube (OVS- 2 tube: 13 mm quartz filter, XAD-2, [270 mg/140 mg]) 30 days if frozen; 7 days at room temperature (24°C) Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap both ends of the tube with plastic caps. Place tube in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 D7645-16 (ASTM) [Tier II] Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 14 days • Acidify to pH < 3.8 with glacial acetic acid • Cool to < 6°C; keep from freezing • Prior to collection of treated water samples, add 10 mg ascorbic acid to sample container Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 538 (EPA OW) [Tier I] Drinking Water 40 mL Amber glass or fluoropolymer container with fIuoropolymer- lined septum or lid 14 days • Prior to collection, add 400 pL of ammonium acetate concentrated stock and 80 [jL of concentrated sodium omadine stock to sample container. [If volumes other than 40-mL are collected, the concentration of ammonium acetate and sodium omadine in the sample should be 1.5 g/L and 64 mg/L, respectively.] • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, liquid, toxic, 6.1, Poison, UN2992 3541 / 3545A (EPA SW-846) / D7645-16 (ASTM) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid Until holding time data are available, analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 3570/8290A Appendix A (EPA SW-846) / D7645-16 (ASTM) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Carbamate pesticides, solid, toxic, 6.1, UN2757 SAM Companion - Sample Collection Information Document A1-67 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 1,4-Thioxane (degradation product of HD) NA Air Not of concern in this sample type EPA/600/R-16/114 (EPA NHSRC) [Tier II] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoropolymer-lined septum 14 days • Cool to < 6°C; keep from freezing For treated water samoles: Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Flammable Liquids, n.o.s, 3, Flammable, UN1993 • Add sodium sulfite to remove residual chlorine151 OR •Add 1.6-2.0 mg NH4CI For all other samples: • Adjust to pH < 2 with 1:1 HCI EPA/600/R-16/114 (EPA NHSRC) [Tier II] Solid 40 mL Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Flammable Solid, n.o.s, 3, Flammable, UN1325 EPA/600/R-16/114 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer screw-cap VOA vial with fluoro polymer-lined septum 14 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film (e.g., fluoro polymer- based), and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Flammable Solid, n.o.s, 3, Flammable, UN1325 Titanium tetrachloride (analyze as total titanium) NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type 3050B / 3051A / 6010D / 6020B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Titanium tetrachloride, 8, Corrosive, UN1838 3051A / 6010D / 6020B (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer or glass container with fluoropolymer-lined lid Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Titanium tetrachloride, 8, Corrosive, UN1838 Triethanolamine (TEA) (degradation product of HN-3) 3509 (NIOSH) [Tier II] Air 0.5 - 300 L (flow rate = 0.5-1 L/min) Impinger (15 mL 2 mM hexanesulfonic acid) [Impinger contents are transferred into VOA vial for 21 days Cool to < 6°C Wipe outside of vial clean using a damp, then dry cloth. Seal the vial with non- reactive tape or film, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 D7599-16 (ASTM) [Tier III - Drinking Water] [Tier II - Non-Drinking Water] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 3541 /3545A (EPA SW-846) / EPA/600/R-11/123 (EPA NHSRC/CDC) [Tier III] Solid 250 mL Wide-mouth amber glass, fluoropolymer or polypropylene container 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 EPA/600/R-11/123 (EPA NHSRC/CDC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass, fluoropolymer or polypropylene container 28 days • Cool to < 6°C • Protect from light Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Ethanolamine solutions, 8, Corrosive, UN2491 SAM Companion - Sample Collection Information Document A1-68 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size<1) Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label131 Trimethyl phosphite TO-10A (EPA ORD) [Tier III] Air 240 - 7200 L (flow rate =1-5 L/min) Sorbent cartridge containing PUF or PUF in combination with other solid sorbent 7 days Cool to < 6°C Remove PUF cartridge from sampler, wrap with aluminum foil used to store cartridge, and place in a sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Trimethyl phosphite, 3, Flammable, UN2329 NA Non-Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type NA Drinking Water For the purposes of this document, this analyte is not considered to be a concern in this sample type 3541 / 3545A / 8270E (EPA SW-846) [Tier III] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Trimethyl phosphite, 3, Flammable, UN2329 3570 / 8290 Appendix A / 8270 E (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Trimethyl phosphite, 3, Flammable, UN2329 1,3,5-Trinitrobenzene (1,3,5-TNB) NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3535A / 8330B (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fiuoro polymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Trinitrobenzeneand trinitrotoluene mixtures, 1.1, Explosive, UN0388™ 8330B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Trinitrobenzeneand trinitrotoluene mixtures, 1.1, Explosive, UN0388™ 3570 / 8290A Appendix A / 8330B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Trinitrobenzeneand trinitrotoluene mixtures, 1.1, Explosive, UN0388|::| SAM Companion - Sample Collection Information Document A1-69 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container*2' Holding Time Preservation Packaging Requirements Shipping Label131 2,4,6-Trinitrotoluene (2,4,6-TNT) NA Air For the purposes of this document, this analyte is not considered to be a concern in this sample type 3535A / 8330B (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 1 L Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Add sodium thiosulfate to remove residual chlorine from treated water samples15' • Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Trinitrobenzeneand trinitrotoluene mixtures, 1.1, Explosive, UN0388™ 8330B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid 14 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Trinitrobenzeneand trinitrotoluene mixtures, 1.1, Explosive, UN0388™ 3570 /8290A Appendix A/ 8330B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Trinitrobenzeneand trinitrotoluene mixtures, 1.1, Explosive, UN0388|::| Vanadium pentoxide (analyze as total vanadium) 10-3.1 / 10-3.4/ 10-3.5 (EPA ORD) [Tier 1] Air Up to 1700 m3 Filter (quartz, silica, or cellulose fiber) 6 months Cool to < 6°C Place filter in protective covering. Place protected filter in double plastic bags, and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Vanadium pentoxide, 6.1, Poison, UN2862 3015A / 6010D / 6020B (EPA SW-846) [Tier I] Non-Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Vanadium pentoxide, 6.1, Poison, UN2862 200.7 / 200.8 (EPA OW) [Tier I] Drinking Water 125 mL Fluoropolymer, plastic or glass container 6 months with acidification. Samples must be acidified within 14 days of collection. • Acidify to pH < 2 with HN03(7) • Acidified samples can be cooled to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Vanadium pentoxide, 6.1, Poison, UN2862 3050B / 3051A / 6010D / 6020B (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Vanadium pentoxide, 6.1, Poison, UN2862 9102 (NIOSH)/ 6010D / 6020B (EPA SW-846) [Tier I] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth fluoropolymer, plastic or glass container Analyze immediately/ as soon as possible Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fluoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Vanadium pentoxide, 6.1, Poison, UN2862 SAM Companion - Sample Collection Information Document A1-70 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 VE [Phosphonothioic acid, ethyl-, S~(2-(diethylamino)ethyl) 0-ethyl ester] TO-17 (EPA ORD) [Tier III] Air 1 - 4 L (flow rate = 16 - 67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier III] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Cool to < 6°C; keep from freezing • Add sodium thiosulfate to remove residual chlorine from treated water samples15' Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier III] Solid 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. SAM Companion - Sample Collection Information Document A1-71 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 VG [Phosphonothioic acid, S- (2-(diethylamino)ethyl) 0,0- diethyl ester] TO-17 (EPA ORD) [Tier III] Air 1 - 4 L (flow rate = 16 - 67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier III] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Cool to < 6°C; keep from freezing • Add sodium thiosulfate to remove residual chlorine from treated water samples15' Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier III] Solid 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. VM [Phosphonothioic acid, methyl-,S-(2- (diethylamino)ethyl) O-ethyl ester] TO-17 (EPA ORD) [Tier III] Air 1 - 4 L (flow rate = 16-67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier III] Drinking Water Non-Drinking Water 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Cool to < 6°C; keep from freezing • Add sodium thiosulfate to remove residual chlorine from treated water samples15' Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier III] Solid 40 ml. Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. SAM Companion - Sample Collection Information Document A1-72 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Analyte Selected Analytical Methods -sAM 2017* Sample Type Sample Size'11 Sample Container'21 Holding Time Preservation Packaging Requirements Shipping Label'31 VX [0-ethyl-S-(2- diisopropylaminoethyl) methyl- phosphonothiolate] TO-17 (EPA ORD) [Tier III] Air 1 - 4 L (flow rate = 16-67 mL/min) Stainless steel, 3.5-inch long tubes with a 6-cm sorbent bed and 1/4-inch o.d. 7 days Cool to < 6°C Wrap tube in aluminum foil, and place in a clean sealed container. Place container in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier I] Drinking Water Non-Drinking Water 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days • Cool to < 6°C; keep from freezing • Add sodium thiosulfate to remove residual chlorine from treated water samples15' Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier II] Solid 40 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. EPA/600/R-16/116 (EPA NHSRC) [Tier II] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid 7 days Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Wrap container with bubble wrap. Place in gallon plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures, then into metal can before placing in cooler. Consult laboratory and/or shipping vendor on specifics regarding shipping label. Wipe outer surface of package with a bleach wipe, taking care not to obscure labeling. White phosphorus 7905 (NIOSH) [Tier I] Air 5 L (at 0.1 mg/m3) - 100 L (flow rate = 0.01 -0.2 L/min) Solid sorbent tube (Tenax GC [100 mg/50 mg]) 7 days Cool to < 6°C Wipe outside of sorbent tube clean using a damp, then dry cloth. Cap tubes with plastic (not rubber) caps. Place tubes in double plastic bags and wrap with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Phosphorus, white (dry or wetted), 4.2, Spontaneously combustible, 6.1, Poison, UN1381 7580 (EPA SW-846) [Tier I] Drinking Water Non-Drinking Water 500 mL Amber glass or fluoropolymer container with fluoropolymer- lined septum or lid; fill to overflowing to eliminate headspace 5 days Cool to < 6°C; keep from freezing Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Phosphorus, white (dry or wetted), 4.2, Spontaneously combustible, 6.1, Poison, UN1381 7580 (EPA SW-846) [Tier I] Solid 250 mL Wide-mouth amber glass or fluoropolymer container with fiuoropolymer-lined septum or lid Samples can be held indefinitely if sealed tightly to eliminate moisture loss Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Phosphorus, white (dry or wetted), 4.2, Spontaneously combustible, 6.1, Poison, UN1381 3570/8290A Appendix A/ 7580 (EPA SW-846) [Tier III] Wipes 1 wipe/100 cm2 or 1 wipe/ft2 Wide-mouth amber glass or fluoropolymer container with fiuoro polymer-lined septum or lid Samples can be held indefinitely if sealed tightly to eliminate moisture loss. • Cool to < 6°C Wipe outside of container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film (e.g., fiuoropolymer-based). Wrap glass containers with bubble wrap. Pack samples as described in Footnote (4). Standard carrier shipping label AND Phosphorus, white (dry or wetted), 4.2, Spontaneously combustible, 6.1, Poison, UN1381 SAM Companion - Sample Collection Information Document A1-73 September 2017 ------- Sample Collection Information Document - Attachment A September 2017 Footnotes: NA Not applicable * EPA's SAM lists the analytical methods that have been selected by multi-agency technical work groups for use by laboratories that are tasked with sample analysis following a contamination incident. The method tiers listed reflect the extent of method validation and indicate the level of method usability for the specific analyte and sample type. These tiers are defined in the Chemical Methods Query on the Selected Analytical Methods for Environmental Remediation and Recovery website: https://www.epa.gov/homeland-securitv-research/sam-chemical-methods-auerv. (1) Sample sizes are based on the minimum amount needed for analysis of a single sample. Additional sample amounts may be requested or needed for laboratory quality control analyses (e.g., duplicates, matrix spikes) or for measurement of low analyte concentrations. It is also recommended that additional sample(s) be collected in case of the need for reanalysis due to sample spillage or unforeseen analytical difficulties. Where possible, sample sizes are based on specifications included in the selected analytical methods. ~ Wipe areas may need to be adjusted based on anticipated analyte concentration. Wipe sample areas are based on U.S. Office of Research and Development, "A Literature Review of Wipe Sampling Methods for Chemical Warfare Agents and Toxic Industrial Chemicals," EPA/600/R-07/004 January 2007. ~ Solid samples should be collected into containers without packing. (2) Use of "certified clean" containers is preferred. Otherwise, use glassware cleaning instructions found in Chapter 3 of SW-846. Rinse with detergent, followed by tap water, 1:1 nitric acid:tap water, 1:1 hydrochloric acid:tap water, and reagent water. Prior to use, wash vials and septa with detergent and rinse with tap and distilled water. Allow the vials and septa to air dry at room temperature, place in a 105°C oven for 1 hr, then remove and allow to cool in an area known to be free of organics. (3) Environmental samples are shipped as nonhazardous cargo if they do not exhibit one of the following U.S. DOT hazard classes: explosive, corrosive, flammable, or poisonous. Only a standard shipping carrier's label is needed in these cases. If any sample is listed under one of these classes, DOT labeling is required. The DOT label must indicate, PSN (49 CFR 172.301), the UN Number (49 CFR 172.301), appropriate hazard class and descriptor (49 CFR 172.101), and "Limited Quantity" when a quantity exception is used (49 CFR 172.101). When the quantity limit for a given substance is exceeded, the phrase "Limited Quantity" should be excluded from the label. Note that there are no "exceptions" for substances belonging to DOT Division 1.1, Division 2.1, Division 2.3 or Division 4.2. Exception quantities include: ~ Exception for Class 3, flammable and combustible liquids: Ensure total net quantity of Class 3 substances does not exceed 1 L (0.3 gal) (49 CFR 173.150) ~ Exception for Division 5.1, oxidizers and Class 9, miscellaneous hazardous materials (see 49 CFR 173.127 for packing group definitions), Packing group II: Ensure the total net quantity does not exceed 1.0 L (0.3 gallons) of liquid or 1.0 kg (2.2 lbs) of solid (49 CFR 173.152); Packing group III: Ensure the total net quantity does not exceed 5 L (1.3 gallons) of liquid or 5.0 kg (11 lbs) of solid (49 CFR 173.152) ~ Exception for Division 6.1, poisonous liquids: Ensure the total net quantity of Class 6.1 liquids does not exceed 4 L (1 gal) of liquid or 5 kg (11 lbs) of solid (49 CFR 173.153) ~ Exception for Class 8, corrosive materials: Ensure total net quantity of Class 8 materials does not exceed 5 L (1.3 gal) of liquid or 5 kg (11 lbs) of solid (49 CFR 173.154) ~ Shipment of samples containing reactive levels (>12%) of energetic material residues using domestic carriers is prohibited. If samples are known or suspected to contain >12% of energetic material residues, ship and label according to DOT requirements for secondary explosive materials. (4) Pack sample transport containers outside the contaminated area. Samples must be packed in a manner that protects the integrity of the samples and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and water-absorbent packing materials or ice (if required for preservation) and shipped in a cooler to ensure sample temperatures do not exceed preservation requirements. If the target analytes are unknown, samples should be maintained at < 6®C, but above freezing. Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the sample containers. (5) Sodium thiosulfate or sodium sulfite should be added to treated drinking water or wastewater samples containing residual chlorine. These dechlorinating agents can be added either prior to or after sample collection, but before sample preservation. ~ For sodium thiosulfate, an amount equivalent to the following is recommended: 3 mg/40-mL sample, or 3 mg/5 mg chlorine residual. ~ For sodium sulfite, an amount equivalent to the following is recommended: for a 1-L sample, add 40 - 50 mg sodium sulfite (1.6 - 2 mg/40-mL sample), either prior to or after collecting the sample. (6) This method applies to ambient concentrations of VOCs above 0.5 ppbv and typically requires VOC enrichment by concentrating up to 1 L of sample volume; however, when using current technologies, quantifications of approximately 100 pptv have been achieved with 0.5-L sample volumes. (7) If sample collectors are not able to preserve samples in the field (e.g., acid preservatives are not available), the samples may be preserved in the laboratory. If preserved in the laboratory, however, samples must be held for at least 24 hours prior to analysis. (8) Samples containing secondary explosives at concentrations less than 10% by weight (100,000 mg/kg) or primary explosives at concentrations less than 2% can be sent as normal environmental samples. Consult laboratory on specifics regarding shipping label and shipping requirements for samples with concentrations greater than these amounts. SAM Companion - Sample Collection Information Document A1-74 September 2017 ------- Attachment B1 Sample Collection Information for the Environmental Samples, Radiochemical Analytes and Methods Listed in SAM 2017 [Note: The acronyms and abbreviations used in this attachment are listed and defined in the beginning of the report.] ------- This page left intentionally blank ------- Sample Collection Information Document - Attachment B1 September 2017 Attachment B1: Sample Collection Information for the Environmental Samples, Radiochemical Analytes and Methods Listed in SAM 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Gross Alpha Air Filter 1 filter (limited to counting geometry size) Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' No preservation required FRMAC, Vol. 2, pg. 33 (DOE) Aqueous & Liquid 2 - 4 L (solids limited) Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' Preserve sample with HN03 or HCI topH < 2 (6) 7110 B (SM) Drinking Water 2 - 4 L (solids limited) Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' Preserve sample with HN03 or HCI topH < 2 (6) 900.0 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' No preservation required AP1 (ORISE) Surface Wipes 1 wipe (limited to counting geometry size) Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' No preservation required FRMAC, Vol. 2, pg. 33 (DOE) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' No preservation required AP1 (ORISE) Gross Beta Air Filter 1 filter (limited to counting geometry size) Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' No preservation required FRMAC, Vol. 2, pg. 33 (DOE) Aqueous & Liquid 2 - 4 L (solids limited) Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' Preserve sample with HNOs or HCI to pH < 2 (6) 7110 B (SM) Drinking Water 2 - 4 L (solids limited) Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' Preserve sample with HNOs or HCI to pH < 2 (6) 900.0 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar, or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' No preservation required AP1 (ORISE) Surface Wipes 1 wipe (limited to counting geometry size) Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' No preservation required FRMAC, Vol. 2, pg. 33 (DOE) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' No preservation required AP1 (ORISE) SAM Companion - Sample Collection Information Document B1 - 1 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Gamma Select Mixed Air Filter 1 filter (limited to counting geometry size) Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic baq Maximum 6 months'4' No preservation required Ga-01-R (HASL-300) Fission Products Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' Preserve sample with HN03 or HCI topH < 2 (6) Ga-01 -R (HASL-300) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' Preserve sample with HN03 or HCI topH < 2 (6) 901.1 (EPA) Soil & Sediment 100-1,000 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' No preservation required Ga-01-R (HASL-300) Surface Wipes 1 wipe (limited to counting geometry size) Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' No preservation required Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' No preservation required Ga-01-R (HASL-300) Total Activity Screening Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' No preservation required Preparation of Samples for Total Activity Screening (Y-12) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' No preservation required Preparation of Samples for Total Activity Screening (Y-12) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' No preservation required Preparation of Samples for Total Activity Screening (Y-12) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' No preservation required. If Tc- 99 is to be assessed, a separate sample will be necessary. Preparation of Samples for Total Activity Screening (Y-12) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' No preservation required. If Tc- 99 is to be assessed, a separate sample will be necessary. Preparation of Samples for Total Activity Screening (Y-12) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' No preservation required. If Tc- 99 is to be assessed, a separate sample will be necessary. Preparation of Samples for Total Activity Screening (Y-12) SAM Companion - Sample Collection Information Document B1 - 2 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Americium-241 (alpha) Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Am-241 half life = 432 years) No preservation required Qualitative: Rapid Methods for Acid or Fusion Digestion (EPA) Confirmatorv: Am-04-RC (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Am-241 half life = 432 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative: D3084-05 (ASTM) Confirmatorv: Am-04-RC (HASL-300) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Am-241 half life = 432 years) Qualitative: No sample preservation is required if sample is delivered to the laboratory within 3 days of collection. If the sample is to be held for more than 3 days, add concentrated HN03 to achieve a pH < 2. Confirmatory: Preserve sample with HN03or HCI to pH < 2 (6) Qualitative: Rapid Radiochemical Method for Am-241 (EPA) Confirmatorv: Am-04-RC (HASL-300) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Am-241 half life = 432 years) No preservation required Qualitative: Rapid Method for Fusion of Soil and Soil-Related Matrices (EPA) Confirmatorv: Am-01-RC (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Am-241 half life = 432 years) No preservation required Qualitative: Rapid Methods for Acid or Fusion Digestion (EPA) Confirmatorv: Am-04-RC (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Am-241 half life = 432 years) No preservation required Qualitative: Actinidesand Sr-89/90 in Vegetation (DOE SRS) Confirmatorv: Am-06-RC (HASL-300) SAM Companion - Sample Collection Information Document B1 - 3 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Americium-241 (gamma) Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Am-241 half life = 432 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Am-241 half life = 432 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: 7120 (SM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Am-241 half life = 432 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Am-241 half life = 432 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Am-241 half life = 432 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Am-241 half life = 432 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Californium-252 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Cf-252 half life = 2.64 years) No preservation required Qualitative: D3084-05 (ASTM) Confirmatorv: Raoid Radiochemical Method for Californium-252 (EPA) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Cf-252 half life = 2.64 years) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative: D3084-05 (ASTM) Confirmatorv: Raoid Radiochemical Method for Californium-252 (EPA) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Cf-252 half life = 2.64 years) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative: D3084-05 (ASTM) Confirmatorv: Rapid Radiochemical Method for Californium-252 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar, or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Cf-252 half life = 2.64 years) No preservation required Qualitative: D3084-05 (ASTM) Confirmatorv: Rapid Radiochemical Method for Californium-252 (EPA) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Cf-252 half life = 2.64 years) No preservation required Qualitative: D3084-05 (ASTM) Confirmatorv: Rapid Radiochemical Method for Californium-252 (EPA) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Cf-252 half life = 2.64 years) No preservation required Qualitative: D3084-05 (ASTM) Confirmatorv: Am-06-RC (HASL-300) SAM Companion - Sample Collection Information Document B1 - 4 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Cesium-137 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Cs-137 half life = 30.17 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Cs-137 half life = 30.17 years) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: 7120 (SM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Cs-137 half life = 30.17 years) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Cs-137 half life = 30.17 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Cs-137 half life = 30.17 years) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Cs-137 half life = 30.17 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Cobalt-60 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Co-60 half life = 5.27 years) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Co-60 half life = 5.27 years) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: 7120 (SM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Co-60 half life = 5.27 years) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Co-60 half life = 5.27 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Co-60 half life = 5.27 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Co-60 half life = 5.27 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) SAM Companion - Sample Collection Information Document B1 -5 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Curium-244 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Cm-244 half life = 18.1 years) No preservation required Qualitative: D3084-05 (ASTM) Confirmatorv: Rapid Radiochemical Method for Curium-244 in Air Particulate Filters, Swipes and Soil (EPA) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Cm-244 half life = 18.1 years) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative: D3084-05 (ASTM) Confirmatorv: Rapid Radiochemical Method for Curium-244 in Air Particulate Filters, Swipes and Soil (EPA) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Cm-244 half life = 18.1 years) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative: D3084-05 (ASTM) Confirmatorv: Rapid Radiochemical Method for Curium-244 in Air Particulate Filters, Swipes and Soil (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Cm-244 half life = 18.1 years) No preservation required Qualitative: D3084-05 (ASTM) Confirmatorv: Raoid Radiochemical Method for Curium-244 in Air Particulate Filters, Swipes and Soil (EPA) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Cm-244 half life = 18.1 years) No preservation required Qualitative: D3084-05 (ASTM) Confirmatorv: Raoid Radiochemical Method for Curium-244 in Air Particulate Filters, Swipes and Soil (EPA) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Cm-244 half life = 18.1 years) No preservation required Qualitative: D3084-05 (ASTM) Confirmatorv: Am-06-RC (HASL-300) SAM Companion - Sample Collection Information Document B1 -6 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Europium-154 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Eu-154 half life = 8.59 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Eu-154 half life = 8.59 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: 7120 (SM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Eu-154 half life = 8.59 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Eu-154 half life = 8.59 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Eu-154 half life = 8.59 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Eu-154 half life = 8.59 years) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) lndium-111 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (ln-111 half life = 2.8 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Borosilicate glass small-mouth bottle Maximum 6 months'4' (ln-111 half life = 2.8 days) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: Ga-01-R (HASL-300) Drinking Water 2 - 4 L Borosilicate glass small-mouth bottle Maximum 6 months'4' (ln-111 half life = 2.8 days) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: 901.1 (EPA) Soil & Sediment 100 g Borosilicate glass wide-mouth jar Maximum 6 months'4' (ln-111 half life = 2.8 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (ln-111 half life = 2.8 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (ln-111 half life = 2.8 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) SAM Companion - Sample Collection Information Document B1 - 7 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' lodine-125 Air Filter 1 charcoal cartridge Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (1-125 half life = 59.4 days) No preservation required Qualitative and Confirmatorv: Procedure #9P) (ORISE) Aqueous & Liquid 2 - 4 L Borosilicate glass small-mouth bottle Maximum 6 months'4' (1-125 half life = 59.4 days) No preservation required Qualitative and Confirmatorv: Procedure #9 (ORISE) Drinking Water 2 - 4 L Borosilicate glass small-mouth bottle Maximum 6 months'4' (1-125 half life = 59.4 days) No preservation required Qualitative and Confirmatorv: Procedure #9 (ORISE) Soil & Sediment 100 g Borosilicate glass wide-mouth jar Maximum 6 months'4' (1-125 half life = 59.4 days) No preservation required. 1-125 will volatilize with heat; store samples at room temperature if stored before shippinq. Qualitative and Confirmatorv: Procedure #9 (ORISE) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (1-125 half life = 59.4 days) No preservation required Qualitative and Confirmatorv: Procedure #9 (ORISE) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (1-125 half life = 59.4 days) No preservation required. 1-125 will volatilize with heat; store samples at room temperature if stored before shipping. Qualitative and Confirmatory: Procedure #9 (ORISE) lodine-131 Air Filter Charcoal or silver zeolite cartridge Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (1-131 half life = 8.03 days) No preservation required Qualitative and Confirmatorv: Ga-01 -R(7) (HASL-300) Aqueous & Liquid 2 - 4 L Borosilicate glass small-mouth bottle Maximum 6 months'4' (1-131 half life = 8.03 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Drinking Water 2 - 4 L Borosilicate glass small-mouth bottle Maximum 6 months'4' (1-131 half life = 8.03 days) No preservation required (Sample acidification recommended in Method 901.1 should not be applied to samples collected for analysis of 1-131) Qualitative and Confirmatorv: 901.1 (EPA) Soil & Sediment 100 g Borosilicate glass wide-mouth jar Maximum 6 months'4' (1-131 half life = 8.03 days) No preservation required. 1-131 will volatilize with heat; store samples at room temperature if stored before shipping. Qualitative and Confirmatorv: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (1-131 half life = 8.03 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (1-131 half life = 8.03 days) No preservation required. 1-131 will volatilize with heat; store samples at room temperature if stored before shipping. Qualitative and Confirmatorv: Ga-01-R (HASL-300) SAM Companion - Sample Collection Information Document B1 -8 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' lridium-192 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (lr-192 half life = 173 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (lr-192 half life = 173 days) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: 7120 (SM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (lr-192 half life = 173 days) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (lr-192 half life = 173 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (lr-192 half life = 173 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (lr-192 half life = 173 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Molybdenum-99 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Mo-99 half life = 65.94 hours) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Mo-99 half life = 65.94 hours) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: Ga-01-R (HASL-300) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Mo-99 half life = 65.94 hours) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Mo-99 half life = 65.94 hours) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Mo-99 half life = 65.94 hours) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Mo-99 half life = 65.94 hours) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) SAM Companion - Sample Collection Information Document B1 -9 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Neptunium-237 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Np-237 half life = 2.14 million years) No preservation required Qualitative and Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Np-237 half life = 2.14 million years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Np-237 half life = 2.14 million years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: 907.0 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Np-237 half life = 2.14 million years) No preservation required Qualitative and Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Np-237 half life = 2.14 million years) No preservation required Qualitative and Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Np-237 half life = 2.14 million years) No preservation required Qualitative and Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Neptunium-239 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Np-239 half life = 2.4 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Np-239 half life = 2.4 days) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: 7120 (SM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Np-239 half life = 2.4 days) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Np-239 half life = 2.4 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Np-239 half life = 2.4 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Np-239 half life = 2.4 days) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) SAM Companion - Sample Collection Information Document B1 -10 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Phosphorus-32 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (P-32 half life = 14.26 days) No preservation required Qualitative and Confirmatorv: RESL P-2 (DOE) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (P-32 half life = 14.26 days) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: R4-73-014 (EPA) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (P-32 half life = 14.26 days) Qualitative: No sample preservation is required if sample is delivered to the laboratory within 3 days of sampling date/time. If the sample is to be held for more than 3 days, add concentrated HN03 to achieve a pH < 2. Confirmatorv: Preserve samole with HNOs or HCI to pH < 2 (6) Qualitative: Rapid Radiochemical Method for P-32 in Water (EPA) Confirmatorv: R4-73-014 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (P-32 half life = 14.26 days) No preservation required Qualitative and Confirmatorv: RESL P-2 (DOE) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (P-32 half life = 14.26 days) No preservation required Qualitative and Confirmatorv: RESL P-2 (DOE) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (P-32 half life = 14.26 days) No preservation required Qualitative and Confirmatorv: RESL P-2 (DOE) SAM Companion - Sample Collection Information Document B1 - 11 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Plutonium-238 Plutonium-239 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Pu-238 half life = 87.7 years) (Pu-239 half life = 24,100 years) No preservation required Qualitative: Rapid Methods for Acid or Fusion Digestion (EPA) Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Pu-238 half life = 87.7 years) (Pu-239 half life = 24,100 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative: D3084-05 (ASTM) Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Pu-238 half life = 87.7 years) (Pu-239 half life = 24,100 years) Qualitative: No sample preservation is required if sample is delivered to the laboratory within 3 days of collection. If the sample is to be held for more than 3 days, add concentrated HN03 to achieve a pH < 2. Confirmatory: Preserve sample with HN03 or HCI to pH < 2 (6) Qualitative: Rapid Radiochemical Method for Pu (EPA) Confirmatorv: EMSL-33 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Pu-238 half life = 87.7 years) (Pu-239 half life = 24,100 years) No preservation required Qualitative: Rapid Method for Fusion of Soil and Soil-Related Matrices (EPA) Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Pu-238 half life = 87.7 years) (Pu-239 half life = 24,100 years) No preservation required Qualitative: Rapid Methods for Acid or Fusion Digestion (EPA) Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Pu-238 half life = 87.7 years) (Pu-239 half life = 24,100 years) No preservation required Qualitative: Actinides and Sr-89/90 in Vegetation (DOE SRS) Confirmatorv: Am-06-RC (HASL-300) SAM Companion - Sample Collection Information Document B1 -12 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Polonium-210 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Po-210 half life = 138 days) No preservation required Qualitative and Confirmatorv: Method 111 (EPA) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Po-210 half life = 138 days) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: Po-02-RC (HASL-300) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Po-210 half life = 138 days) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: Po-02-RC (HASL-300) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Po-210 half life = 138 days) No preservation required Qualitative and Confirmatorv: Po-02-RC (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Po-210 half life = 138 days) No preservation required Qualitative and Confirmatorv: Method 111 (EPA) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Po-210 half life = 138 days) No preservation required Qualitative and Confirmatorv: Po-02-RC (HASL-300) Radium-223 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Ra-223 half life = 11.4 days) No preservation required Qualitative and Confirmatorv: Rapid Radiochemical Method for Ra-226 (EPA) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Ra-223 half life = 11.4 days) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: Rapid Radiochemical Method for Ra-226 (EPA) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Ra-223 half life = 11.4 days) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatorv: Rapid Radiochemical Method for Ra-226 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Ra-223 half life = 11.4 days) No preservation required Qualitative and Confirmatorv: Rapid Radiochemical Method for Ra-226 (EPA) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Ra-223 half life = 11.4 days) No preservation required Qualitative and Confirmatorv: Rapid Radiochemical Method for Ra-226 (EPA) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Ra-223 half life = 11.4 days) No preservation required Qualitative and Confirmatorv: Rapid Radiochemical Method for Ra-226 (EPA) SAM Companion - Sample Collection Information Document B1 -13 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Radium-226 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Ra-226 half life = 1,600 years) No preservation required Qualitative: Rapid Methods for Acid or Fusion Digestion (EPA) Confirmatorv: Raoid Radiochemical Method for Radium-226 in Building Materials (EPA) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Ra-226 half life = 1,600 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative: 7500-Ra B (SM) Confirmatorv: 7500-Ra C (SM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Ra-226 half life = 1,600 years) Qualitative: No sample preservation is required if sample is delivered to the laboratory within 3 days of collection. If the sample is to be held for more than 3 days, add concentrated HN03 to achieve a pH < 2. Confirmatorv: Preserve samole with HNOs or HCI to pH < 2 (6) Qualitative: Raoid Radiochemical Methods for Ra-226 (EPA) Confirmatorv: 903.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Ra-226 half life = 1,600 years) No preservation required Qualitative: Raoid Method for Radium in Soil (EPA) Confirmatorv: AP7 (ORISE) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Ra-226 half life = 1,600 years) No preservation required Qualitative: Raoid Methods for Acid or Fusion Digestion (EPA) Confirmatorv: Rapid Radiochemical Method for Radium-226 in Building Materials (EPA) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Ra-226 half life = 1,600 years) No preservation required Qualitative and Confirmatorv: Ra-03-RC (HASL-300) SAM Companion - Sample Collection Information Document B1 - 14 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Rhenium-188 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months (Re-188 half life = 16.9 hours) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months (Re-188 half life = 16.9 hours) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatory: Ga-01-R (HASL-300) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months (Re-188 half life = 16.9 hours) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatory: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months (Re-188 half life = 16.9 hours) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months (Re-188 half life = 16.9 hours) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months (Re-188 half life = 16.9 hours) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Rubidium-82 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Rb-82 half life = 75 seconds) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Rb-82 half life = 75 seconds) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatory: Ga-01-R (HASL-300) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Rb-82 half life = 75 seconds) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatory: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Rb-82 half life = 75 seconds) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Rb-82 half life = 75 seconds) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Rb-82 half life = 75 seconds) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) SAM Companion - Sample Collection Information Document B1 -15 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Ruthenium-103 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Ru-103 half life = 39.3 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Ru-103 half life = 39.3 days) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatory: 7120 (SM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Ru-103 half life = 39.3 days) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatory: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Ru-103 half life = 39.3 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Ru-103 half life = 39.3 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Ru-103 half life = 39.3 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Ruthenium-106 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Ru-106 half life = 374 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Ru-106 half life = 374 days) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatory: 7120 (SM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Ru-106 half life = 374 days) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatory: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Ru-106 half life = 374 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Ru-106 half life = 374 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Ru-106 half life = 374 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) SAM Companion - Sample Collection Information Document B1 -16 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Selenium-75 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Se-75 half life = 120 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Se-75 half life = 120 days) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatory: 7120 (SM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Se-75 half life = 120 days) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatory: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Se-75 half life = 120 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Se-75 half life = 120 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Se-75 half life = 120 days) No preservation required Qualitative and Confirmatory: Ga-01-R (HASL-300) Strontium-89 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Sr-89 half life = 50.53 days) No preservation required Qualitative and Confirmatory: Strontium in Food and Bioenvironmental Samples (EPA) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Sr-89 half life = 50.53 days) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatory: 905.0 (EPA) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Sr-89 half life = 50.53 days) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative and Confirmatory: 905.0 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Sr-89 half life = 50.53 days) No preservation required Qualitative: Actinidesand Sr-89/90 in Soil Samples (DOE SRS) Confirmatory: Strontium in Food and Bioenvironmental Samples (EPA) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Sr-89 half life = 50.53 days) No preservation required Qualitative and Confirmatory: Strontium in Food and Bioenvironmental Samples (EPA) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Sr-89 half life = 50.53 days) No preservation required Qualitative: Actinidesand Sr-89/90 in Soil Samples (DOE SRS) Confirmatory: Strontium in Food and Bioenvironmental Samples (EPA) SAM Companion - Sample Collection Information Document B1 -17 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Strontium-90 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Sr-90 half life = 28.8 years) No preservation required Qualitative: Rapid Methods for Acid or Fusion Digestion (EPA) Confirmatorv: Sr-03-RC (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Sr-90 half life = 28.8 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: D5811-08 (ASTM) Drinking Water 2 - 4 L Samples should be collected in 1 -L plastic containers Maximum 6 months'4' (Sr-90 half life = 28.8 years) Qualitative: No sample preservation is required if sample is delivered to the laboratory within 3 days of sampling date/time. If the sample is to be held for more than 3 days, add concentrated HN03 to achieve a pH < 2. Confirmatorv: Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative: Rapid Radiochemical Methods for Sr-90 (EPA) Confirmatorv: 905.0 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Sr-90 half life = 28.8 years) No preservation required Qualitative: Rapid Method for Sodium Carbonate Fusion of Soil and Soil- Related Matrices (EPA) Confirmatorv: Sr-03-RC (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Sr-90 half life = 28.8 years) No preservation required Qualitative: Rapid Methods for Acid or Fusion Digestion (EPA) Confirmatorv: Sr-03-RC (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Sr-90 half life = 28.8 years) No preservation required Qualitative: Actinides and Sr-89/90 in Vegetation (DOE SRS) Confirmatorv: Sr-03-RC (HASL-300) SAM Companion - Sample Collection Information Document B1 - 18 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Technetium-99 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Tc-99 half life = 211,000 years) No preservation required Qualitative and Confirmatorv: AP5 (ORISE) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Tc-99 half life = 211,000 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: D7168-05 (ASTM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Tc-99 half life = 211,000 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: Tc-02-RC (HASL-300) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Tc-99 half life = 211,000 years) No preservation required Qualitative and Confirmatorv: AP5 (ORISE) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Tc-99 half life = 211,000 years) No preservation required Qualitative and Confirmatorv: AP5 (ORISE) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Tc-99 half life = 211,000 years) No preservation required Qualitative: AP5 (ORISE) Confirmatorv: Tc-01-RC (HASL-300) Technetium-99m Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Tc-99m half life = 6 hours) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Tc-99m half life = 6 hours) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Tc-99m half life = 6 hours) No preservation required Qualitative and Confirmatorv: 901.1 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Tc-99m half life = 6 hours) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Tc-99m half life = 6 hours) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Tc-99m half life = 6 hours) No preservation required Qualitative and Confirmatorv: Ga-01-R (HASL-300) SAM Companion - Sample Collection Information Document B1 -19 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Thorium-227 Thorium-228 Thorium-230 Thorium-232 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Th-227 half life = 18.7 days) (Th-228 half life = 1.9 years) (Th-230 half life = 7.5 years) (Th-232 half life = 1.4 x 1010 years) No preservation required Qualitative and Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Th-227 half life = 18.7 days) (Th-228 half life = 1.9 years) (Th-230 half life = 7.5 years) (Th-232 half life = 1.4 x 1010 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' (Th-227 half life = 18.7 days) (Th-228 half life = 1.9 years) (Th-230 half life = 7.5 years) (Th-232 half life = 1.4 x 1010 years) Preserve sample with HN03 or HCI topH < 2 (6) Qualitative and Confirmatorv: 907.0 (EPA) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' (Th-227 half life = 18.7 days) (Th-228 half life = 1.9 years) (Th-230 half life = 7.5 years) (Th-232 half life = 1.4 x 1010 years) No preservation required Qualitative and Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' (Th-227 half life = 18.7 days) (Th-228 half life = 1.9 years) (Th-230 half life = 7.5 years) (Th-232 half life = 1.4 x 1010 years) No preservation required Qualitative and Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (Th-227 half life = 18.7 days) (Th-228 half life = 1.9 years) (Th-230 half life = 7.5 years) (Th-232 half life = 1.4 x 1010 years) No preservation required Qualitative and Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) SAM Companion - Sample Collection Information Document B1 - 20 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Tritium (Hydrogen-3) Air Filter For the purposes of this document, this analyte is not considered to be a concern in this sample type Aqueous & Liquid 2 - 4 L Borosilicate glass small-mouth bottle Maximum 6 months'4' (3H half life = 12.32 years) No preservation required Qualitative and Confirmatorv: 906.0 (EPA) Drinking Water 2 - 4 L Borosilicate glass small-mouth bottle Maximum 6 months'4' ("H half life = 12.32 years) No preservation required Qualitative and Confirmatorv: 906.0 (EPA) Soil & Sediment 100 g Borosilicate glass wide-mouth jar Maximum 6 months'4' ("H half life = 12.32 years) No preservation required. Tritium will volatilize with heat; store samples at room temperature if stored before shipping. Qualitative and Confirmatorv: AP2 (ORISE) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' ("H half life = 12.32 years) No preservation required. Tritium will volatilize with heat; do not dry sample before analysis. Qualitative and Confirmatorv: AP2 (ORISE) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' (3H half life = 12.32 years) No preservation required. Tritium will volatilize with heat; do not dry sample prior to analysis. Qualitative and Confirmatorv: AP2 (ORISE) SAM Companion - Sample Collection Information Document B1 - 21 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected'3' Sample Container'4' Holding Time'4' Sample Preservation'4' Analytical Method'5' Uranium-234 Uranium-235 Uranium-238 Air Filter 1 filter Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' ~ (U-234 half life = 244,000 years) ~ (U-235 half life = 700 x 106 years) ~ (U-238 half life = 4.47 x 109 years) No preservation required Qualitative: Rapid Methods for Acid or Fusion Digestion (EPA) Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Aqueous & Liquid 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' ~ (U-234 half life = 244,000 years) ~ (U-235 half life = 700 x 106 years) ~ (U-238 half life = 4.47 x 109 years) Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative: 7500-U B (SM) Confirmatorv: 7500-U C (SM) Drinking Water 2 - 4 L Plastic (polypropylene or polyethylene) or borosilicate glass small-mouth bottle Maximum 6 months'4' ~ (U-234 half life = 244,000 years) ~ (U-235 half life = 700 x 106 years) ~ (U-238 half life = 4.47 x 109 years) Qualitative: No sample preservation is required if sample is delivered to the laboratory within 3 days of collection. If the sample is to be held for more than 3 days, add concentrated HN03 to achieve a pH < 2. Confirmatory: Preserve sample with HNOs or HCI to pH < 2 (6) Qualitative: Rapid Radiochemical Method for U (EPA) Confirmatorv: D3972-02 (ASTM) Soil & Sediment 100 g Plastic (polypropylene or polyethylene) large-mouth bottle, jar or plastic bag. Borosilicate glass wide-mouth jar may also be used. Maximum 6 months'4' ~ (U-234 half life = 244,000 years) ~ (U-235 half life = 700 x 106 years) ~ (U-238 half life = 4.47 x 109 years) No preservation required Qualitative: Rapid Method for Fusion of Soil and Soil-Related Matrices (EPA) Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Surface Wipes 1 wipe Glassine envelope, polyacrylic plastic Petri dish, or polyethylene plastic bag Maximum 6 months'4' ~ (U-234 half life = 244,000 years) ~ (U-235 half life = 700 x 106 years) ~ (U-238 half life = 4.47 x 109 years) No preservation required Qualitative: Rapid Methods for Acid or Fusion Digestion (EPA) Confirmatorv: SOP for Actinides in Environmental Matrices (EPA-NAREL) Vegetation 1 -gallon tightly packed zipper-locked bag or plastic container Plastic (polypropylene or polyethylene) bag with zipper lock and durability to resist punctures OR wide-mouth plastic container Maximum 6 months'4' ~ (U-234 half life = 244,000 years) ~ (U-235 half life = 700 x 106 years) ~ (U-238 half life = 4.47 x 109 years) No preservation required Qualitative: Actinides and Sr-89/90 in Vegetation (DOE SRS) Confirmatorv: U-02-RC (HASL-300) SAM Companion - Sample Collection Information Document B1 - 22 September 2017 ------- Sample Collection Information Document - Attachment B1 September 2017 Footnotes: (1) Sample transport containers are packed outside the contaminated area. Samples must be packed in a manner that protects the integrity of the containers. Samples should be surrounded by shock absorbing and liquid absorbent packing materials (49 CFR 173.24 and 173.453). Check the radiation-specific activity from the packaged samples (49 CFR 173.403). (2) Although the package required for transporting radioactive material is based on the activity INSIDE the package, the label required on the transport package is based on the radiation hazard OUTSIDE the package. Radioactive material is the only hazardous material that has three possible labels, depending on the relative radiation levels external to the package. Labels for radioactive material also require a Transportation Index (Tl) number which indicates the highest radiation level measured at 1 meter from the surface of the package. The three possible labels are commonly called White 1, Yellow 2, and Yellow 3, referring to the color of the label and the number prominently displayed. The specific label required (White 1, Yellow 2, or Yellow 3) is based on the limits specified in 49 CFR 172.403. (3) Sample sizes are provided for guidance, and may vary depending on the specific contamination incident, data quality objectives and requirements, and laboratory radiochemistry license considerations. With the exception of total gamma, the sample sizes listed are based on the amount needed to support analyses addressing all the radioisotopes listed in this attachment; the sample size listed for total gamma is based on the amount needed to determine only total gamma radiation. (4) Sample container, holding times, preservation, and sample preparation information has been derived from requirements stated in the analytical methods, documents cited under "Analytical Method" or in Section 7.0 of this document, or best professional judgment. Holding times can be significantly less than 6 months, depending on the specific isotope targeted and its concentration in the sample. (5) Methods listed in this column can be located using U.S. Environmental Protection Agency, National Homeland Security Research Center's (NHSRC) Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2017 (https://www.epa.aov/homeland-securitv-research/samV The SAM document is intended to be used concurrently with this Sample Collection Information Document. Full citations for references not cited and/or accessed through the SAM website are provided in Section 7.0 of this document. (6) If dissolved components are to be determined, samples requiring preservation must be filtered prior to preservation. Drinking water samples can be preserved in the laboratory within 5 days of collection, if sample collectors are not able to preserve the samples in the field (7) This procedure should be used only for filters specifically designed for iodine. SAM Companion - Sample Collection Information Document B1 -23 September 2017 ------- This page left intentionally blank ------- Attachment B2 Sample Collection Information for the Outdoor Building and Infrastructure Materials, Radiochemical Analytes and Methods Listed in SAM 2017 [Note: The acronyms and abbreviations used in this attachment are listed and defined in the beginning of the report.] ------- This page left intentionally blank ------- Sample Collection Information Document - Attachment B2 September 2017 Attachment B2: Sample Collection Information for the Outdoor Building and Infrastructure Materials, Radiochemical Analytes and Methods Listed in Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected131 Sample Container*4' Holding Time*4' Sample Preservative*4' Sample Preparation Methods*5' Analytical Methods*5' Asphalt shingles 250 grams Chips, rubble, particulates: 250-mL or larger plastic jars or sample bottles Cores, cuttings, bulk Maximum 6 months (Am-241 half life = 432 years) No preservation required • Rapid Method for Sodium Hydroxide Fusion of Asphalt Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses • EPA NAREL - Rapid Radiochemical Method for Americium-241 in Building Materials Asphalt paving material 25 grams materials: Large zip-locking or plastic bag with ties, and/or box (steel, wood or fiberboard) No preservation required • Rapid Method for Sodium Hydroxide Fusion of Asphalt Roofing Material Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses Concrete 25 grams No preservation required • Rapid Method for Sodium Hydroxide Fusion of Concrete and Brick 25 grams No preservation required Brick Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses for Environmental Remediation Following Radiological Incidents Granite 25 grams No preservation required Stucco 25 grams No preservation required Plutonium-238 Plutonium-239 Asphalt shingles 250 grams Chips, rubble, particulates: 250-mL or larger plastic jars or sample bottles Cores, cuttings, bulk materials: Large zip-locking or plastic bag with ties, and/or box (steel, wood or fiberboard) Maximum 6 months (Pu-238 half life = 87.7 years) (Pu-239 half life - 24,100 years) No preservation required ~ Rapid Method for Sodium Hydroxide Fusion of Asphalt Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses • EPA NAREL - Rapid Radiochemical Method for Plutonium-238 and Plutonium- 239/240 in Building Materials Asphalt 25 grams No preservation required • Rapid Method for Sodium Hydroxide Fusion of Asphalt Roofing Material Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses Concrete 25 grams No preservation required • Rapid Method for Sodium Hydroxide Fusion of Concrete and Brick 25 grams No preservation required Brick Matrices Prior to Americium, Plutonium, Strontium, Radium, and Granite 25 grams No preservation required Uranium Analyses for Environmental Remediation Following Radiological Incidents Stucco 25 grams No preservation required Sample Collection Information Document B2-1 September 2017 ------- Sample Collection Information Document - Attachment B2 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected131 Sample Container*4' Holding Time*4' Sample Preservative*4' Sample Preparation Methods*5' Analytical Methods*5' Radium-226 Asphalt shingles 250 grams Chips, rubble, particulates: 250-mL or larger plastic jars or sample bottles Cores, cuttings, bulk materials: Large zip-locking Maximum 6 months (Ra-226 half life = 1,600 years) No preservation required • Rapid Method for Sodium Hydroxide Fusion of Asphalt Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses • EPA NAREL - Radium-226 in Bitumen, Aggregate, Stone or other Solid Samples: Rapid Method for High-Activity Samples Asphalt 25 grams or plastic bag with ties, and/or box (steel, wood or fiberboard) No preservation required • Rapid Method for Sodium Hydroxide Fusion of Asphalt Roofing Material Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses • EPA NAREL - Rapid Radiochemical Method for Radium-226 in Building Materials for Environmental Remediation Following Radiological Incidents Concrete 25 grams No preservation required • Rapid Method for Sodium Hydroxide Fusion of Concrete and Brick Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses for Environmental Remediation Following Radiological Incidents • EPA NAREL - Rapid Radiochemical Method for Radium-226 in Building Materials for Environmental Remediation Following Radiological Incidents Brick 25 grams No preservation required Granite 25 grams No preservation required Stucco 25 grams No preservation required Stontium-90 Asphalt shingles 250 grams Chips, rubble, particulates: 250-mL or larger plastic jars or sample bottles Cores, cuttings, bulk materials: Large zip-locking or plastic bag with ties, and/or box (steel, wood or fiberboard) Maximum 6 months (Sr-90 half life = 28.8 years) No preservation required ~ Rapid Method for Sodium Hydroxide Fusion of Asphalt Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses • EPA NAREL - Rapid Radiochemical Method for Strontium-90 (Sr-90) in Building Materials for Environmental Remediation Following Radiological Incidents Asphalt 25 grams No preservation required • Rapid Method for Sodium Hydroxide Fusion of Asphalt Roofing Material Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses Concrete 25 grams No preservation required • Rapid Method for Sodium Hydroxide Fusion of Concrete and Brick 25 grams No preservation required Brick Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses for Environmental Remediation Following Radiological Incidents Granite 25 grams No preservation required Stucco 25 grams No preservation required Sample Collection Information Document B2 -2 September 2017 ------- Sample Collection Information Document - Attachment B2 September 2017 Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples outside the contaminated area as described in Footnote (1). Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2). Analyte Sample Type Sample Size Collected131 Sample Container*4' Holding Time*4' Sample Preservative*4' Sample Preparation Methods*5' Analytical Methods*5' Uranium-234 Uranium-235 Uranium-238 Asphalt shingles 250 grams Chips, rubble, particulates: 250-mL or larger plastic jars or sample bottles Cores, cuttings, bulk materials: Large zip-locking or plastic bag with ties, and/or box (steel, wood or fiberboard) Maximum 6 months (U-234 half life = 244,000 years) (U-235 half life - 700 million years) (U-238 half life = 4.47 billion years) No preservation required • Rapid Method for Sodium Hydroxide Fusion of Asphalt Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses • Rapid Method for Sodium Hydroxide Fusion of Asphalt Roofing Material Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses • EPA NAREL - Rapid Radiochemical Method for Isotopic Uranium in Building Materials for Environmental Remediation Following Radiological Incidents Asphalt 25 grams No preservation required Concrete 25 grams No preservation required • Rapid Method for Sodium Hydroxide Fusion of Concrete and Brick Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium Analyses for Environmental Remediation Following Radiological Incidents Brick 25 grams No preservation required Granite 25 grams No preservation required Stucco 25 grams No preservation required Footnotes: (1) Sample transport containers are packed outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers. Samples should be surrounded by shock absorbing and liquid absorbent packing materials (49 CFR 173.24 and 173.453). Check the radiation-specific activity from the packaged samples (49 CFR 173.403). (2) Although the package required for transporting radioactive material is based on the activity INSIDE the package, the label required on the transport package is based on the radiation hazard OUTSIDE the package. Radioactive material is the only hazardous material that has three possible labels, depending on the relative radiation levels external to the package. Labels for radioactive material also require a Transportation Index (Tl) number which indicates the highest radiation level measured at 1 meter from the surface of the package. The three possible labels are commonly called White 1, Yellow 2, and Yellow 3, referring to the color of the label and the number prominently displayed. The specific label required (White 1, Yellow 2, or Yellow 3) is based on the limits specified in 49 CFR 172.403. (3) Sample sizes are provided for guidance, and may vary depending on the specific contamination incident, data quality objectives and requirements, and laboratory radiochemistry license considerations. The sample sizes listed are based on the amount needed to support analyses addressing all the radioisotopes listed in this attachment. (4) Sample container, holding times, preservation, and sample preparation information has been derived from requirements stated in the analytical methods, documents cited under "Analytical Method" or in Section 7.0 of this document, or best professional judgment. Holding times can be significantly less than 6 months, depending on the specific isotope targeted and its concentration in the sample. (5) Methods listed in this column can be located using U.S. Environmental Protection Agency, National Homeland Security Research Center's (NHSRC) Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2017 (https://www.epa.aov/homeland-securitv-research/samV The Selected Analytical Methods document is intended to be used concurrently with this Sample Collection Information Document. Full citations for references not cited and/or accessed through the SAM website are provided in Section 7.0 of this document. Sample Collection Information Document B2 -3 September 2017 ------- This page left intentionally blank ------- Attachment C Sample Collection Information for the Biotoxins and Methods Listed in SAM 2017 [Note: The acronyms and abbreviations used in this attachment are listed and defined in the beginning of the report.] ------- This page left intentionally blank ------- Sample Collection Information Document - Attachment C September 2017 Attachment C: Sample Collection Information for the Biotoxin Analytes and Methods Listed in SAM 2017 Analyte(s) Selected Analytical Methods - SAM 2017* Sample Type Sampling Device / Medium Sample Container Size111 Preservation Holding Time Packaging Shipping Label'2' Abrin and Abrine (abrin marker) Presumptive: ~ [LFA] Adapted from Biosecurity and Bio terrorism: Biodefense Strategy, Practice, and Science (2014) 12(1): 49-62 [Tier I / Tier II] ~ as Abrine [LC-MS-MS] EPA 600/R-13/022 [Tier I/Tier II] Confirmatorv: ~ [ELISA and ECL] Adapted from Journal of Food Protection (2008) 71(9): 1868-1874 [Tier II] Bioloaical Activity: ~ [Enzyme Activity] Adapted from Analytical Biochemistry (2008) 378(1): 87-89 [Tier II] Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. ~ Avoid freeze-thaw cycles during transport of samples collected for abrin. NOTE: For detection and measurement of abrine in treated water samples, add the following to the sample container prior to collection: ~ 8 mg sodium thiosulfate/100-mL sample ~ 6.4 mg sodium omadine /100-mL sample Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. NOTE: 28 days for abrine in water Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnote (5). See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50 -100 ml_ Water (surface water, waste water, drinking water) Sterile leak proof container NOTE: Collect water samples for analysis of abrine in polypropylene container with polyethylene screw-cap. 100 mL Aflatoxins (B1, B2, G1, G2) Presumptive and Confirmatorv: ~ [Immunoaffinity (column) purification / HPLC-FL] Adapted from 991.31 (AOAC) [Tier II] Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group I See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50 -100 mL Water (surface water, waste water, drinking water) Sterile leak proof container 100 mL SAM Companion - Sample Collection Information Document C1 -1 September 2017 ------- Sample Collection Information Document - Attachment C September 2017 Analyte(s) Selected Analytical Methods - SAM 2017* Sample Type Sampling Device / Medium Sample Container Size111 Preservation Holding Time Packaging Shipping Label'2' a-Amanitin Presumptive: ~ [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II] Confirmatorv: ~ [LC-MS-MS] EPA 600/R-13/022 (EPA) [Tier II] Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. NOTE: For treated water: ~ Add 8 mg sodium thiosulfate and 6.4 mg sodium omadine /100- mL sample. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. NOTE: 28 days for water samples Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group II See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50-100 g Water (surface water, waste water, drinking water) Polypropylene container with polyethylene screw- cap 100 mL Anatoxin-a Presumptive: ~ ELISA [Tier III] (No reference is available at this time. Please consult the SAM website* for information regarding the status of this assay.) Confirmatorv: ~ [LC-ESI-MS-MS] Method 545 (EPA) [Tier I/Tier II] Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter. Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. NOTE: Use amber alass or amber PETG containers (or protect from light) See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. ~ Protect from light NOTE: For treated water samples, add 1 g sodium bisulfate and 0.1 g ascorbic acid / 1-L sample. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. NOTE: 28 days for water samples cooled to < 10°C Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnote (5). Belongs to Packing Group II See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) Particulate (swabs, wipes, dust socks) Sterile leak proof container - amber glass or amber PETG (or protect from light) At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks Solid (soil, powder) Sterile leak proof container - amber glass or amber PETG (or protect from light) 50 -100 mL Water (surface water, waste water, drinking water) Sterile leak proof container - amber glass or amber PETG (or protect from light) 100 mL SAM Companion - Sample Collection Information Document C1 - 2 September 2017 ------- Sample Collection Information Document - Attachment C September 2017 Analyte(s) Selected Analytical Methods - SAM 2017* Sample Type Sampling Device / Medium Sample Container Size111 Preservation Holding Time Packaging Shipping Label'2' Botulinum neurotoxins (Serotypes A - G) Presumptive: ~ [LFA] Adapted from EPA Environmental Technology Verification report [Tier I/Tier II] ~ [ECL] Adapted from Journal of the Science of Food and Agriculture (2014) 94: 707-712 [Tier II] ~ [Fluorescent bead-based assay] Multi-agency Report. Rapid Botulinum Toxin Assay Test, Evaluation and Validation Study Report [Tier III] ~ [Antibody capture FRET- based activity] Adapted from Analytical Biochemistry (2011) 411: 200-209 [Tier II] Confirmatorv: Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. ~ Avoid freeze-thaw cycles during transport. NOTE: For treated water, add 50 mg sodium thiosulfate / L sample (DO NOT add sodium thiosulfate to samples that will be analyzed using MS) Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group I See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50 -100 ml_ ~ [LC-ESI-MS-MS / MALDI- TOF MS] Adapted from Analytical Chemistry (2005) 77: 3916-3924 [Tier II] Bioloaical Activity: ~ [Mouse Bioassay] APHA Press Compendium of Methods, Chapter 32 [Tier I] Water (surface water, waste water, drinking water) Sterile leak proof container 100 mL SAM Companion - Sample Collection Information Document C1 - 3 September 2017 ------- Sample Collection Information Document - Attachment C September 2017 Analyte(s) Selected Analytical Methods - SAM 2017* Sample Type Sampling Device / Medium Sample Container Size111 Preservation Holding Time Packaging Shipping Label'2' Brevetoxins (A and B Forms) Presumptive and Confirmatorv: • [ELISA / LC-MS] Adapted from Toxicon (2015) 96: 82-88 [Tier II] Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group II See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxic solids, organic, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) Particulate (swabs, wipes, dust socks) Sterile, leak proof container At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50 -100 ml_ Water (surface water, waste water, drinking water) Sterile leak proof container 100 mL Cylindrospermopsin Presumptive: ~ [ELISA] Adapted from Environmental Sciences and Technology (2010) 44: 7361-7368 [Tier II] Confirmatorv: ~ [LC-ESI-MS-MS] Method 545 (EPA) [Tier I/Tier II] Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. NOTE: Use amber alass or amber PETG containers (or protect from light) See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. NOTE: For treated water, add 1 g sodium bisulfate and 0.1 g ascorbic acid / 1-L sample. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. NOTE: 28 days for water samples cooled to < 10°C Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnote (5). This substance does not require specific labeling for transport Particulate (swabs, wipes, dust socks) Sterile leak proof container - amber glass or amber PETG (or protect from light) At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks Solid (soil, powder) Sterile leak proof container - amber glass or amber PETG (or protect from light) 50 -100 mL Water (surface water, waste water, drinking water) Amber glass container with fluoropolymer- lined screw cap 100 mL SAM Companion - Sample Collection Information Document C1 - 4 September 2017 ------- Sample Collection Information Document - Attachment C September 2017 Analyte(s) Selected Analytical Methods - SAM 2017* Sample Type Sampling Device / Medium Sample Container Size111 Preservation Holding Time Packaging Shipping Label'2' Diacetoxyscirpenol (DAS) Presumptive: ~ [ELISA] Adapted from Journal of Food Microbiology (1988) 6(1): 9-17 [Tier III] Confirmatorv: ~ [LC-MS-MS] Adapted from Food Research International (2015) 72: 247-255 [Tier II] Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group II See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50 -100 mL Water (surface water, waste water, drinking water) Sterile leak proof container 100 mL Domoic acid Presumptive: ~ [ELISA] Adapted from Journal of AOAC International (2007) 90(4): 1011-1027 [Tier II] ~ [ELISA] Adapted from Journal of Shellfish Research (2008) 27(5): 1301-1310 [Tier II] Confirmatorv: ~ [LC-UV] Adapted from Journal of the Mexican Chemical Society (2011) 55(2): 65-71 [Tier II] Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnote (5). This substance does not require specific labeling for transport Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50-100 mL Water (surface water, waste water, drinking water) Sterile leak proof container A minimum of 100 mL SAM Companion - Sample Collection Information Document C1 - 5 September 2017 ------- Sample Collection Information Document - Attachment C September 2017 Analyte(s) Selected Analytical Methods - SAM 2017* Sample Type Sampling Device / Medium Sample Container Size111 Preservation Holding Time Packaging Shipping Label'2' Microcystins (LA, LF, LR, LY, RR, YR) Presumptive: ~ [ELISA] Method 546 (EPA) [Tier I/Tier II] Confirmatorv: Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature < 10°C; do not freeze] ~ Protect from light NOTE: Prior to collection, add the following to containers used to collect treated water samples: ~ For samples to be analyzed using ELISA (Method 546), 100 mg sodium thiosulfate / 1-L sample. ~ For samples to be analyzed using LC-MS- MS (Method 544), add 1) 7.75 g Trizma, 2) 2 g 2- chloroacetamide, 3) 100 mg ascorbic acid, 4) 0.35 g ethylenediamine- tetraacetic acid trisodium salt /1-L sample. Method 544 analysis: 28 days Method 546 analysis: 48 hours (14 days if frozen within 48 hours) Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group I See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) ~ [LC-MS-MS] Method 544 (EPA) [Tier I/Tier II] contents into sterile leak proof container for shipping. NOTE: Use amber alass or amber PETG containers (or protect from light) Particulate (swabs, wipes, dust socks) Sterile leak proof container - amber glass or amber PETG (or protect from light) At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks Solid (soil, powder) Sterile leak proof container - amber glass or amber PETG (or protect from light) 50 -100 ml_ Water (surface water, waste water, drinking water) Sterile leak proof container - amber glass or amber PETG (or protect from light) 500 mL Picrotoxin Confirmatorv: ~ [LC-UV] Adapted from Journal of Pharmaceutical and Biomedical Analysis (1989) 7(3): 369-375 [Tier II] Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. ~ Protect from light. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group II See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50 -100 mL Water (surface water, waste water, drinking water) Sterile leak proof container 100 mL SAM Companion - Sample Collection Information Document C1 - 6 September 2017 ------- Sample Collection Information Document - Attachment C September 2017 Analyte(s) Selected Analytical Methods - SAM 2017* Sample Type Sampling Device / Medium Sample Container Size111 Preservation Holding Time Packaging Shipping Label'2' Ricin and Ricinine (ricin marker) Presumptive: ~ [LFA] Adapted from Biosecurity and Bio terrorism: Biodefense Strategy, Practice, and Science (2013) 11(4): 237-250 [Tier I] ~ [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II] ~ [ECL] Adapted from Journal of AOAC International (2008) 91(2): 376-382 [Tier II] ~ as Ricinine [LC-MS-MS] EPA 600/R-13/022 (EPA/CDC) [Tier I/Tier II] Confirmatorv and Bioloaical Activity: ~ [Isotope Dilution LC-MS / MALDI-TOF MS] Adapted from Analytical Chemistry (2011) 83: 2897-2905 [Tier II] Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. ~ Avoid freeze-thaw cycles during transport of samples collected for ricin. NOTE: For treated water, add 50 mg sodium thiosulfate / 1-L sample NOTE: For samples Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group III See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes, swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50 -100 ml_ collected for detection and measurement of ricinine in treated water, add the following to the sample container prior to collection: ~ 8 mg sodium thiosulfate/100mL sample ~ 6.4 mg sodium omadine / 100-mL sample Water (surface water, waste water, drinking water) Sterile leak proof container 100 mL SAM Companion - Sample Collection Information Document C1 - 7 September 2017 ------- Sample Collection Information Document - Attachment C September 2017 Analyte(s) Selected Analytical Methods - SAM 2017* Sample Type Sampling Device / Medium Sample Container Size111 Preservation Holding Time Packaging Shipping Label'2' Saxitoxins (STX, NEOSTX, dcSTX, dcNEOSTX, doSTX, GTX1-6, dcGTX 1-4) Presumptive: ~ [Receptor binding assay] Method 2011.27 (AOAC) [Tier II] ~ [ELISA] Adapted from Toxicon (2009) 54: 313- 320 [Tier II] and (water samples) Harmful Algae (2016) 56: 77-90 [Tier I] Confirmatorv: Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. NOTE: Use amber alass or amber PETG containers (or protect from light) See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. NOTE: For treated water, add 100-1,000 mg/L sodium thiosulfate or 100 mg/L ascorbic acid NOTE: For untreated Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group I See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) • [HILIC MS-MS] Adapted from Journal of Chromatography A (2015) 1387: 1-12 [Tier II] freshwater samples to be analyzed using ELISA kits, 10X concentrated diluent is added to the sample container prior to or immediately following sample collection. Therefore, coordinate with receiving laboratory(ies) prior to sample collection. Particulate (swabs, wipes, dust socks) Sterile leak proof container - amber glass or amber PETG (or protect from light) At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks Solid (soil, powder) Sterile leak proof container - amber glass or amber PETG (or protect from light) 50 -100 ml_ Water (surface water, waste water, drinking water) Sterile leak proof container - amber glass or amber PETG (or protect from light) A minimum of 100 mL Shiga and Shiga-like Toxins Presumptive: ~ [ELISA] Adapted from Austin Immunology (2016) 1(2): 1007:1-7 [Tier I/Tier II] Confirmatorv: ~ [LC-MS-MS] Adapted from Analytical Chemistry (2014) 86: 4698-4706 [Tier II] Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.® Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. ~ Avoid freeze-thaw cycles during transport. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution.. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group I See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, liquid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50 -100 mL Water (surface water, waste water, drinking water) Sterile leak proof container 100 mL SAM Companion - Sample Collection Information Document C1 - 8 September 2017 ------- Sample Collection Information Document - Attachment C September 2017 Analyte(s) Selected Analytical Methods - SAM 2017* Sample Type Sampling Device / Medium Sample Container Size111 Preservation Holding Time Packaging Shipping Label'2' Staphylococcal enterotoxins (SEA, SEB, SEC, SED, SEE) Presumptive: ~ [ELFA] 2007.06 (AOAC) [Tier II] ~ [ECL] Adapted from Journal of AOAC International (2014) 97(3): 862-867 [Tier III] Confirmatorv: Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. ~ Avoid freeze-thaw cycles during transport. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group I See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) ~ [ELISA] Adapted from Letters in Applied Microbiology (2011) 52: 468-474 [Tier II] Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50 -100 mL Water (surface water, waste water, drinking water) Sterile leak proof container 100 mL T-2 Mycotoxin (T-2, HT-2) Presumptive: ~ [ELISA] Adapted from Journal of Food Protection (2005) 68(6): 1294-1301 [Tier II] Confirmatorv: Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. ImDinaer: Transfer contents into sterile leak proof container for shipping. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. NOTE: For treated water. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group I Aircraft: Cargo only See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) ~ [LC-MS] Adapted from Rapid Communications in Mass Spectrometry (2006) 20(9): 1422-1428 [Tier II] add 8 mg sodium thiosulfate and 6.4 mg sodium omadine /100- mL sample Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50 -100 mL Water (surface water, waste water, drinking water) Sterile leak proof container 100 mL SAM Companion - Sample Collection Information Document C1 -9 September 2017 ------- Sample Collection Information Document - Attachment C September 2017 Analyte(s) Selected Analytical Methods - SAM 2017* Sample Type Sampling Device / Medium Sample Container Size111 Preservation Holding Time Packaging Shipping Label'2' Tetrodotoxin Presumptive: ~ [Receptor binding assay] Method 2011.27 (AOAC) [Tier II] Confirmatorv: Aerosol (filter/cassette, liquid impinger)'3' Filter: Sterile MCE or PTFE filter.(3) Place the filter in a sterile leak proof container. See Footnote (4) ~ Immediately place on ice (e.g., ice packs, secure double-bagged ice). Target temperature <10°C. Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area as described in Footnotes (5) and (6). Belongs to Packing Group I See Footnote (2) for environmental samples. For neat materials: Label outer package with the proper shipping name: "Toxins, extracted from living sources, solid, n.o.s." AND ~ UN# 2811 (inhalation hazard), ~ UN# 3172 (liquid hazard), or ~ UN# 3462 (solid hazard) ~ [LC-MS-MS] Adapted from Toxicon (2016) 119: 64-71 [Tier II] contents into sterile leak proof container for shipping. Particulate (swabs, wipes, dust socks) Sterile leak proof container At least 2 sterile, synthetic, and moistened wipes or swabs, or dust socks Solid (soil, powder) Sterile leak proof container 50 -100 mL Water (surface water, waste water, drinking water) Sterile leak proof container 100 mL Footnotes: * Methods listed in this column can be located using the SAM website (https://www.epa.aov/homeland-securitv-research/selected-analvtical-methods-environmental-remediation-and-recoven/-sarTl. Full citations for references not cited and/or accessed through this document or its website are provided in Section 7.0. Analytical technologies and methods addressing biotoxins continue to be developed and improved; the contact information on this website should be used for updates regarding analytical procedures. (1) Sample sizes are provided for guidance, and may vary depending on the specific contamination incident, data quality objectives and requirements, and laboratory needs. If requested by the laboratory, additional sample(s) must be collected for analyses using multiple methods, or for laboratory quality control analyses (e.g., duplicates, matrix spikes). It is also recommended that additional sample(s) be collected in cases where low concentrations are expected or in the case of an anticipated need for reanalysis due to sample spillage or unforseen analytical difficulties. (2) The exterior of the package must be labeled with the proper shipping name and the UN Number (49 CFR 172.301). For packages meeting the small quantity exception (49 CFR 173.153), label the exterior of the package as follows: "This package conforms to 49 CFR 173.4." No other hazard labeling is required. Packaging must meet the minimum standards as described in 49 CFR 173.4. Environmental samples meet the small quantities exception for Division 6.1 (poisonous) materials if the maximum quantity of material per inner receptacle or article is limited to—(i) Thirty (30) mL (1 ounce) for authorized liquids, other than Division 6.1, Packing Group I, Flazard Zone A or B materials; (ii) Thirty (30) g (1 ounce) for authorized solid materials; (iii) One (1) g (0.04 ounce) for authorized materials meeting the definition of a Division 6.1, Packing Group I, Flazard Zone A or B material. Environmental samples sufficiently diluted so as to no longer meet the standards of a poisonous material (49 CFR 173.132, Class 6, Division 6.1 - Definitions) do not require hazard labeling. For packages using dry ice to maintain temperature preservation requirements, attach a dry ice label to the exterior of the packaging, inform the shipper of the weight of the dry ice, and the name and address of the shipper and recipient. (3) Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sample collectors also should consult the Sample Collection Plan (SCP), Health and Safety Plan (FIASP) and/or analytical laboratory to determine the appropriate air collection filter(s). (4) Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sample collectors should consult the incident-specific Sample Collection Plan (SCP), Health and Safety Plan (FIASP) and/or analytical laboratory for appropriate volumes. (5) Sample transport containers are packed outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers and provides temperature conditions required for sample preservation. Samples should be surrounded by shock- and enough water-absorbent packing material to absorb sample contents. If required for preservation, samples should be surrounded by ice or dry ice in a cooler to ensure sample temperatures do not exceed temperature requirements. If dry ice is used, the outer packaging must be appropriately labeled (Footnote (3). Ice should be placed in separate plastic bags or cold packs should be used to avoid leakage, and the bags placed around, among, and on top of the secondary sample containers. Further guidance can be obtained from 49 CFR 173.199 http://edocket.access.apo.aov/cfr 2002/octatr/pdf/49cfr173.199.pdft and 42 CFR 72 and 73 (http://oig.hhs,go v/authorities/docs/05/032905FRselecta gents, pdfl. (6) Flazardous substances belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 - 173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4. Sample collectors also should consult the incident-specific SCP for instructions regarding shipping labels. SAM Companion - Sample Collection Information Document C1 -10 September 2017 ------- This page left intentionally blank ------- &EPA United States Environmental Protection Agency PRESORTED STANDARD POSTAGE & FEES PAID EPA PERMIT NO. G-35 Office of Research and Development Homeland Security Research Program Cincinnati, OH 45268 ------- |