March 2019 Update: EPA has validated and published a rapid method for sodium hydroxide fusion of
limestone matrices prior to americium, plutonium, strontium, radium and uranium analyses. The
method is summarized and accessible through the link provided below.
Rapid Method for Sodium Hydroxide Fusion of Limestone Matrices Prior to Americium, Plutonium,
Strontium, Radium, and Uranium Analyses for Environmental Remediation Following Radiological
Incidents
Analyte(s)
CAS RN®
Americium-241
14596-10-2
Plutonium-238
13981-16-3
Plutonium-239
15117-48-3
Radium-226
13982-63-3
Strontium-90
10098-97-2
Uranium-234
13966-29-5
Uranium-235
15117-96-1
Uranium-238
7440-61-1
Analysis Purpose: Sample Preparation
Technique: Alpha spectrometry
Method Developed for: Americium-241 (241Am), plutonium-238 (238Pu), plutonium-239 (239Pu), radium-
2 26 ( 226Ra), strontium-90 (90Sr), uranium-234 (234U), uranium-2 3 5 ( 235U) and uranium-23 8 ( 238U) in
limestone samples.
Method Selected for: Selected Analytical Methods for Environmental Remediation and Recovery (SAM)
now lists this method for qualitative analysis of americium-241 (241Am), plutonium-23 8 ( 238Pu),
plutonium-239 (239Pu), radium-2 26 ( 226Ra), strontium-90 (90Sr), uranium-234 (234U), uranium-235 (235U)
and uranium-238 (238U) in limestone samples.
Description of Method: This is a general method for limestone samples collected following a radiological
or nuclear incident. The limestone samples may be received as core samples, crushed samples, or in
pieces of various sizes. Limestone samples should be crushed and pulverized (milled) to achieve a
particle size small enough that representative subsamples can be taken and representative aliquants
analyzed. After a homogeneous subsample is obtained, the limestone sample aliquant is taken and
fused to digest the limestone sample matrix, and matrix removal steps are employed to collect and
preconcentrate the radionuclides from the alkaline fusion matrix. The method is based on the rapid
dissolution of a representative, finely milled aliquants of approximately 1 g of limestone using sodium
hydroxide fusion at 600 °C. Plutonium (Pu), uranium (U) and americium (Am) are separated from the
alkaline matrix using an iron hydroxide/titanium hydroxide precipitation followed by a lanthanum
fluoride matrix removal step. Strontium (Sr) is separated from the alkaline matrix using a phosphate
precipitation followed by a calcium fluoride precipitation to remove silicates. Radium (Ra) is separated
from the alkaline matrix using a carbonate precipitation. This method is a sample digestion and
pretreatment technique to be used prior to the following separation and analysis methods:
U.S. Environmental Protection Agency
Office of Research and Development, Homeland Security Research Program
EPA 600/S-19/024
March 2019

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•	U.S. EPA. Rapid Radiochemical Method for Americium-241 in Building Materials for Environmental
Remediation Following Radiological Incidents (EPA 402-R14-007, April 2014)
•	U.S. EPA. Rapid Radiochemical Method for Plutonium-238 and Plutonium-239/240 in Building
Materials for Environmental Remediation Following Radiological Incidents (EPA 402-R14-006, April
2014)
•	U.S. EPA. Improved Rapid Radiochemical Method for Radium-226 in Building Materials for
Environmental Remediation Following Radiological Incidents (Revision 1, EPA 402-S17-002, May
2017)
•	U.S. EPA. Rapid Radiochemical Method for Total Radiostrontium (Sr-90) in Building Materials for
Environmental Remediation Following Radiological Incidents (EPA 402-R14-00, April 2014)
•	U.S. EPA. Rapid Radiochemical Method for Isotopic Uranium in Building Materials for Environmental
Remediation Following Radiological Incidents (EPA 402-R14-005, April 2014)
Special Considerations:
Limestone samples with larger particle size may require a longer fusion time. Samples with elevated
activity or samples that require multiple analyses from a single aliquant may need to be split after
dissolution. In these cases, the initial digestate and the split fractions should be carefully measured to
ensure that the sample aliquant for analysis is accurately determined.
Information regarding the elemental composition of the sample may be helpful. For example, limestone
may have native concentrations of uranium, radium, thorium (Th), strontium or barium (Ba), any of
which may have an effect on the chemical separations used following the fusion of the sample. In some
cases (e.g., strontium analysis), elemental analysis of the digestate prior to chemical separations may be
necessary to determine native concentrations of carrier elements present in the sample. The amount of
stable strontium added to the limestone samples is designed to minimize the impact from native stable
strontium.
Matrix blanks for these matrices may not be practical to obtain. Efforts should be made to obtain
independent, analyte-free materials that have similar composition as the samples to be analyzed. These
blanks will serve as process monitors for the fusion process, and as potential monitors for cross
contamination during batch processing.
Uncontaminated limestone material may be acceptable blank material for Pu, Am and Sr analyses, but
this material will contain background levels of naturally occurring U and Ra isotopes.
Additional information regarding potential interferences and procedures for addressing the
interferences is provided in Section 4 of the sodium hydroxide fusion method listed below.
Source: U.S. EPA, National Air and Radiation Environmental Laboratory. "Rapid Method for Sodium
Hydroxide Fusion of Limestone Matrices Prior to Americium, Plutonium, Strontium, Radium, and Uranium
Analyses for Environmental Remediation Following Radiological Incidents" (EPA 402-R-18- 002, August
2018) https://www.epa.gov/sites/production/files/2018-08/documents/limestone method final.pdf
U.S. Environmental Protection Agency
Office of Research and Development, Homeland Security Research Program
EPA 600/S-19/024
March 2019

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