EPA 950-R-19-002 /May 2019
Regional
Laboratory
Network
2018 Annual Report





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Regional Laboratory Network
2018 Annual Report | Page 5 of 94
Guam
Trust Territories
American Samoa
Northern Mariana
Islands
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Figure 1, Map of the 10 EPA Regions






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Regional Laboratory Network	2018 Annual Report | Page 11 of 94
Providing for Clean and Safe Water
EPA works with states, tribes, territories; and local communities to better safeguard human
health; maintain, restore, and improve water quality; and make America's water systems
sustainable and secure, supporting new technology and innovation wherever possible.
Emergency Response to Hurricanes
The Region 4 Science and Ecosystem Support Division
(SESD) Regional Laboratory supported the Bay County
Florida residents during the devastation of Hurricane
Michael. SESD deployed the regional mobile
laboratory and provide field inspections to support
local drinking water and wastewater plants, SESD
engineers conducted Infrastructure Assessments for
the wastewater facilities that were inoperable due to
the hurricane and developed Assessment Reports that
were used for FEMA and Bay County to estimate the
cost of the damages. SESD microbiologists analyzed 411 drinking water samples for total
coliform and E. coli to support the drinking water facilities that were unable to analyze
samples. This support was critical to end the Boiling Water Alert in the area and ensure
that residents had access to safe drinking water.
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Regional Laboratory Network
2018 Annual Report | Page 14 of 94
Acid mine drainage is caused when
water (rain water, groundwater,
snowmelt, pond water) flows over
and through sulfur-bearing materials
forming sulfuric acid. Heavy metals
can be leached from rocks by contact
with the resulting acidic waters.
Ultimately, acid mine drainage causes
environmental degradation that
includes damaging impacts to aquatic
resources in the receiving waters. In
Region 9, over 1.5 million data points
were collected at 23 monitoring stations associated with several acid mine drainage sites in
2018. Satellite and cellular telemetry connected to the stations allows real-time year-
round water quality and flow monitoring by site Remedial Project Managers from their
desktops.
•	Argonaut Mine - Continuous monitoring data (12 locations, 505,520 data points)
collected at the Argonaut Mine in Jackson,
CA was used to develop engineering
controls to minimize acid mine drainage
and prevent an environmentally
catastrophic mine spill. The Argonaut
Mine Site is a former hard rock gold mine
in Jackson, CA. Portions of the site's soil
have high levels of arsenic, lead, mercury,
and other metals. Waste from the mine is
held back by a concrete arch dam. The
dam was determined to be unstable in
2015 with the potential for a catastrophic
failure that could have resulted in loss of
life and $100 million in damages. EPA is coordinating with the California
Environmental Protection Agency's (CalEPA) Department of Toxic Substances Control
(DTSC) to stabilize the concrete arch dam and to construct a stormwater diversion
system behind the dam, to prevent water from pooling and increasing the load on the
dam. This site was listed on the National Priorities List in September 2016.
•	Copper Bluff - In 2018, EPA proposed the addition of the Copper Bluff Mine Site to the
National Priorities List. The continuous water level, flow and conductivity data (1
location, 70,080 data points) collected by the Region 9 Laboratory's field team was a
key contributing element to the decision-making process. The Copper Bluff Mine is an
abandoned copper, zinc, silver and gold mine located within the Hoopa Valley Indian
Reservation. The mine's primary entrance, or adit, is 300 feet above the Trinity River.
The Trinity River is federally recognized as a "Wild and Scenic River." Heavy metals
released from the mine could be hazardous to the tribal subsistence fishery in the

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Regional Laboratory Network
2018 Annual Report | Page 15 of 94
Trinity River and the habitat for the threatened Coho Salmon. In addition, Tribal and
non-tribal pedestrians are exposed to the acid mine drainage and the associated orange
sludge on a variety of frequented paths within the Reservation.
•	Iron Mountain Mine - Iron Mountain Mine (IMM) is a former iron, silver, gold, copper,
zinc and pyrite mine. The installation and operation of a full-scale neutralization
system and the construction and operation of the Slickrock Creek Retention Reservoir
to collect contaminated runoff for treatment have significantly reduced acid and metal
contamination in surface waters at the site. The continuous water quality and flow
monitoring systems installed in the watershed by the Region 9 Laboratory provide real
time data (5 stations, 525,600 data points) on the efficacy of the treatment systems and
the variability of the quality of waters discharging from the site. The discharges, via
Spring Creek, flow to the Sacramento River, the drinking water source for 35% of
California. More recently, the stations have started monitoring increases in turbidity
due to wildfire damage in the watershed.
•	Leviathan Mine - Leviathan Mine is an abandoned open-pit sulfur mine on the eastern
slope of the Sierra Nevada. The mine is 24 miles southeast of Lake Tahoe and acid mine
drainage has contaminated a 9-
mile stretch of mountain
creeks. Continuous water
quality and flow monitoring
provided by the Region 9
Laboratory was initially used to
characterize the variability of
water quality leaving the site at
various time scales: daily,
monthly and seasonally. As in
the case of IMM, after the
implementation of treatment
measures, the monitoring (4
locations, 420,480 data points)
has been used to evaluate the efficacy and consistency of the treatment systems and to
provide a check on the water quality of the receiving waters.
•	Sulphur Bank Mercury Mine - The Sulphur Bank Mercury Mine is an abandoned open
pit mercury mine that is located on the shoreline of Clear Lake in California. The mine
introduced mercury to bottom sediments in Clear Lake, and the levels of mercury in fish
from the lake led the State to issue an advisory to limit consumption of fish. Real time
continuous depth data (1 location, 70,080 data points) collected by the Region 9
Laboratory in the Herman Impoundment, a flooded acidic open pit on the site, is
monitored to check for potential overflow into Clear Lake during severe rain events.
The monitoring enabled emergency response personnel to add a berm to the outflow
structure that prevented such a discharge this past winter.


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Regional Laboratory Network
2018 Annual Report | Page 17 of 94
Southside Chattanooga Lead Site
Region 4 SESD conducted a Remedial
Investigation (RI) at the Southside
Chattanooga Lead (SCL) Site in
Chattanooga, Hamilton County, Tennessee.
The site consisted of residential
neighborhoods and communal areas (such
as schools, parks, and daycare facilities}
where lead-impacted foundry material has
been used as fill material and topsoil. The
investigation focused on eight
neighborhoods in the downtown
Chattanooga area: Alton Park, Cowart
Place, East Lake, Highland Park, Jefferson Heights, Oak Grove, Richmond, and Southside
Gardens.
The investigation was conducted to define the nature and extent of lead contamination at
the site. The collected data was utilized to assess the potential human and ecological risks
that may be resulted from exposure to the contaminated materials. Additionally, soil XRF
data were used by the On-Scene Coordinator to identify properties above the emergency
response action level.
Ensuring Safety of Chemicals
Chemicals and pesticides released into the environment as a result of their manufacture,
processing, use, or disposal can threaten human health and the environment. EPA gathers
and assesses information about the risks associated with chemicals and pesticides to allow
more rapid and accurate assessment
Suspicious Cattle Deaths Associated with Coumaphos Application
Coumaphos (found in Co-Ral) is an effective pesticide that has been widely used in Texas to
kill ticks on cattle which cause Cattle Fever (bovine babesiosis). Cattle had been
quarantined in 8 counties in south Texas along the Mexican border due to the severity of
Cattle Fever in the area. In order to treat these cattle, the animal is put into a small
enclosure, called a spray box, so that the treatment spray (Co-Ral) can be administered and
contained inside the box.
Several cattle died after receiving this treatment, which led to an investigation by the Texas
Agriculture Commissioner (TAC) into the cause. One of the main suspects for the deaths




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Regional Laboratory Network
2018 Annual Report | Page 21 of 94
Wastewater Treatment Plant Operators Workshop for Central America
The WWTP Operators Workshop provided training to WWTP operators from Honduras, El
Salvador, Costa Rica, and Dominican Republic. The workshop also included visits to three
wastewater treatment plants (Los Tajos WWTP, Lindora WWTP and Santa Ana WWTP) to
perform field exercise activities (dissolved oxygen measurement profile, pH readings,
settlometer tests, etc.). The workshop was part of an ongoing effort between USEPA and
The United States Agency for International Development (USAID), under an USEPA-USAID
Participating Agency Service Agreement (PASA), to support the environmental obligations
under the CAFTA-DR and the Environmental Cooperation Agreement (ECA),
The workshop also helped develop the ability of local operators to address WWTP process
control and operational activities effectively, thus supporting the protection of the water
quality of the waterways. Several countries benefited from the workshop presentations
and field activities. In addition, a network of professionals was developed to continue to
support a joint effort to develop sustainable wastewater treatment systems and
strengthening of the wastewater management in the Region.
On August 2018, Region 4 SESD engineers conducted the first Wastewater Treatment
Plant (WWTP) Operators Workshop for the
Dominican Republic- Central America Free Trade
Agreement (CAFTA-DR). The workshop consisted
of discussions concerning the design, maintenance,
process control, operation and management factors
limiting treatment performance on municipal
wastewater treatment plants and provide
recommendations to improve plant operation and
performance. USEPA-SESD has been supporting the
CAFTA-DR environmental projects with technical
leads of wastewater regulations workshops and diagnostic evaluations of WWTPs.

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Regional Laboratory Network
2018 Annual Report | Page 22 of 94
Environmental Sustainability in CAFTA-DR and Panama
Note: Information referenced from "The Regional CAFTA-DR Environmental Program from USAID,
Building A Sustainable Future" 2018 Brochure.
The people of the United States, through the United States Agency for International
Development (USAID), using the framework of the Free Trade Agreement between Central
America, Dominican Republic, the United States (CAFTA-DR), and Panama have contributed
to the prosperity of the signatory countries and improved the environmental performance
of the region. Based on this agreement, Central America and Dominican Republic are
advancing towards reaching their goal of sustainable development, improving the
environmental standards in order to favor economic growth and having the most minimal
impact possible on the environment.
As a result of the environmental cooperation of the United States Environmental Protection
Agency (USEPA- Region 4 SESD) and The United States Agency for International
Development (USAID) in the CAFTA-DR context, Central America and Dominican Republic
are utilizing a regional Standard Waste Water Model as a template, which is a scientific
methodology that established 12 basic elements for developing and implementing
regulatory programs for the discharge of effluents. In this regard, the countries have
initiated a process of discussion and socialization of the standard at the public-private
levels, so they can slowly begin to lay the foundations in order to adapt this regional
instrument to national regulations.
USEPA - Region 4 developed
technical reports for each
treatment plant identifying
opportunities for optimizing
operations to improve treatment
and energy use efficiency.
Cocabel, Wastewater Treatment Plant, Lempira, Honduras
USEPA-Region 4 SESD helped to improve the scientific capacity of regional laboratories in
accurately determining the quality of the wastewater discharge. This project strengthened
the technical skills and knowledge of the staff from 12 laboratories from Guatemala,
Honduras, El Salvador, Nicaragua, Costa Rica, Panama and Dominican Republic. This made

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Regional Laboratory Network
2018 Annual Report | Page 23 of 94
it possible to develop an inter-comparison process among laboratories and to work with
samples to determine the efficiency and assertiveness of the results from each of them.
This work allowed for a pre-certification of laboratories and helped establish a wastewater
reference network between the CAFTA-DR countries and Panama that will help to increase
the sustainability of water resources in the region.
The laboratories were selected according to their high institutional commitment to train
others, their operational quality control systems, their satisfactory participation in tests
among different laboratories, the technical competency of their personnel, their adequate
training and laboratory infrastructure, as well
as their hierarchical organizational position
that does not generate conflicts of interest.
Water and Physical-Chemical Services Laboratory,
Universidad de Chiriqui, Panama.
7th Africa Water Week/ African Ministers Council on Water Conference
The Laboratory Capacity Building Team (Region 4
LSASD and ORD Cincinnati) composed 3
presentations for the 7th Africa Water Week/
African Ministers Council on Water Conference in
Libreville, Gabon. These presentations described
the results of a joint US Environmental Protection
Agency/ The United States Agency for
International Development (USAID)/Ghana Water
Company Limited (GWCL) Drinking Water
Laboratory Capacity program. The overall goal
was to ensure the delivery of clean water in urban areas by building the capacity of
laboratories for sampling and analysis of water. Ensuring the validity and reliability of data
through a documented quality system protects public health by providing a sound scientific



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Regional Laboratory Network
2018 Annual Report | Page 26 of 94
The first priority was to fill data gaps associated with a human health risk assessment of
exposure to sediment and surface water for tribal use scenarios. The sampling events were
a coordinated effort led by EPA Region 6, but involved sampling conducted by Region 7,
Oklahoma State agencies, tribal members and others. Over 1400 samples were taken and
analyzed resulting in 8,353 results from 14 sample sets for this site.
There were 17 separate analytical methods requested, with a focus
on metals (lead) and mercury. Region 7 chemists ground and
analyzed the 400 tissue samples (plant, various mammals, fish,
frogs, mussels) providing a service to the field teams and saving the
agency money. Nine months later to meet the screening criteria for
human health risk assessments, the Project Manager requested that
all the results be modified to report down to the method detection
limits instead of the originally reported limits.
The analyses performed by the Region 7 laboratory resulted in a
$104,700 commercial value per year. The data transmitted to the
project managers provided the risk data for the states and tribes. Additionally, the data will
be incorporated into a final Human Health Risk Assessment, then to a Remedial
Investigation and further into remedy decisions for two watersheds and the four Superfund
sites of the Tri-State Mining District (covering two EPA regions, three states, and several
tribes).
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Regional Laboratory Network
2018 Annual Report | Page 31 of 94
The study had multiple unique characteristics including long duration, site-specific
meteorology, measurement of multiple air pollutant species, and use of a wide variety of
sampling methods and instrumentation including; traditional Federal Reference methods
(FRM), lower-cost sensor packages, citizen science, and mobile monitoring techniques.
The six fixed monitoring sites employed FRM devices incorporating both Teflon and Quartz
fiber filters to measure both PM2.5
and elemental carbon/organic
carbon (EC/OC). Non FRM sampling
included a lower cost sensor
package, called the P-POD,
employing sensors streaming 1-
minute PM2.5 and EC/OC
concentration data. The P-POD adds
particulate matter to the sensor pod
(S-PQD). Additionally, beta
attenuation monitors (EBAMs) were
incorporated at selected fixed
monitoring sites. The citizen science monitoring program used portable, battery powered
units logging 10-second PM2.5 data, and the mobile monitoring campaign employed an
instrumented electric vehicle, GMAP (Geospatial Measurement of Air Pollution), recording
1-second ultrafine PM and BC (black carbon) data.
The citizen science monitors were based on EPA's Air
Mapper design. The original Air Mapper design/build
concept was significantly modified and updated by
Region 7 scientists incorporating 3-D printing
technology to locally manufacture an inventory of Air
Mappers sufficient to support multiple deployments of
citizen scientists over several weeks. The Air Mappers
employed rechargeable battery power, global
positioning system, optical particle sensor estimating
PM1/PM2.5/PM10, C02, and other sensors including accelerometer, noise, temperature,
and relative humidity.
In total, this one-year data collection and monitoring effort yielded over 385 million data
points that will be used for instrument intercomparison and source characterization
purposes. The collection of a large volume of data, coupled with high time and spatial
resolution meteorology, PM and BC concentrations allows the researchers to document and
ascertain the potential impacts from many source categories. Region 7 scientists will
continue to collaborate with ORD researchers during the data analysis phase of this study
to further elucidate the relative impact of a wide variety of transportation related sources
in these Kansas City, KS neighborhoods.


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Regional Laboratory Network
2018 Annual Report | Page 32 of 94
Improving Efficiency and Effectiveness
EPA is working to modernize and improve business processes and operations to promote
transparency, efficiency, and effectiveness, and improve the capabilities and cost-effectiveness
of its information technology and information management systems.
Quality Assurance Guidance for Citizen Science
The Region 1 Office of Environmental Measurement and Evaluation's Quality Assurance
Unit, in conjunction with the Headquarters Office of Research and Development and Office
of Mission Support, led a national initiative in developing quality assurance guidance for
citizen science projects. The purpose of the guidance is to provide citizen scientists with
tools and procedures that can help document the quality of data to achieve an intended
purpose. The guidance should be released to the public in the Spring of 2019.
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Lab Efficiencies and Effectiveness
Region 7 chemists and support staff embraced LEAN and ELMS activities to improve
laboratory performance while responding to customer needs. Their most notable
accomplishment this year was to submit all sample data on-time with a standard 30 day
turn-around. They improved their performance using lean tools, focusing on
Program/State mission results, improving customer service, and being accountable
resource stewards.

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Regional Laboratory Network
2018 Annual Report | Page 33 of 94
For the last four years, the team has conducted 2-3 mini-
lean events annually to understand and improve
operations resulting in a savings of $30,000 per year on
sample container procurement and streamlined data
reviews to less thanlO days. They used an ELMS flow
board to track and revise over 80 SOPs within a two-
month period. They have begun the process to transition
to electronic data review and analysis transmittals to
save agency paper procurement to the tune of 600 boxes
annually. Our laboratory chemists constantly look for
ways to improve the analytical methods which reduce
preparation times and hazardous waste costs. The team
supported a new extraction for semi-volatile compounds
which requires no solvents, and which has lower
detection limits with smaller sample aliquots.
The team's analytical products are used by the programs
for enforcement, monitoring and human health
evaluations. This year, 100% of the data generated by
EPA was delivered to the customer on time with an
average delivery of 12 days before the due date and with
a standard delivery of 30 days. Over 50 of the 370
projects had shorter due dates of 3 or 7 days. This year,
the chemists completed the analysis of 17,266 samples
generating 262,255 results meeting all the agency
required quality controls. 100% of the CLP sample data
was reviewed for completeness and acceptability.
In each of the LEAN events, the team invited customers to participate so that their needs
were incorporated. One improvement was to request one electronic survey to respond to
RCRA disposal guidance and customer service, replacing two paper forms. Initial
responses were low, and the team developed talking points to be shared with customers.
At the end of the year, the team receives greater than 95% of the surveys with a single
reminder. Any complaints were addressed quickly. Out of 433 responses, only 10 had
complaints; less than 2% complaint rate.
Because the team is focused on the return on investments that the programs have made,
they are looking for ways to be good stewards of EPA's resources while addressing the
region's needs. Towards that end, they have eliminated 12 rarely used analytical methods.
They have partnered with the states in the region, analyzing quarterly water samples
saving Nebraska over $45,000 annually. They have implemented sample analysis for
Pesticide Enforcement Inspections with one new method incorporating many analytes.
They continue to analyze fish tissue samples for health advisories, completing 875 samples
SOP huddle board in the first month
SOP huddle board after 2 months,
completing 80 SOP revisions.






























































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