PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
A EPA
June 2019
United States	Office of Chemical Safety and
Environmental Protection Agency	Pollution Prevention
Environmental Hazard
Extended Data Extraction
Supplemental Document
For Draft Risk Evaluation of
Cyclic Aliphatic Bromide Cluster
(HBCD)
CASRN
NAME
25637-99-4
Hexabromocyclododecane
3194-55-6
1,2,5,6,9,10-Hexabromocyclododecane
3194-57-8
1,2,5,6-Tetrabromocyclooctane
June, 2019
1

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
Table of Contents
Table 1. On-topic aquatic toxicity studies that were evaluated for HBCD	3
Table 2. On-topic terrestrial toxicity studies that were evaluated for HBCD	54
2

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
Tabke 1. On-topic aquatic toxicity studies that were evaluated for HBCD
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
Aquatic Vegetation
25637-99-4
Green algae
(Pseudokirc
hneriella
subcapitata)
Fresh
24-hour
ECio = >0.0037
mg AI/L (0.0037 is
the mean of the
Day 0 and Day 4
6.8 mg/L
measurements)
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022, 3.38,
0.0042, 0.0064
mg/L (measured,
Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance
(cell density);
Population
growth rate
(area under
growth curve)
{Wildlife Intl
LTD, 1997,
1928298@@au
thor-year}
High
1928298
25637-99-4
Green algae
(P seudokirchne
riella
subcapitata)
Fresh
24-hour
EC50 = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance (cell
density);
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High
25637-99-4
Green algae
(Pseudokirchne
riella
subcapitata)
Fresh
24-hour
EC90 = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance (cell
density);
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High
25637-99-4
Green algae
(Pseudokirchne
riella
subcapitata)
Fresh
48-hour
EC10 = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance (cell
density);
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High
25637-99-4
Green algae
(Pseudokirchne
riella
subcapitata)
Fresh
48-hour
EC50 = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance (cell
density);
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High
3

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Green algae
(P seudokirchne
riella
subcapitata)
Fresh
48-hour
EC90 = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance (cell
density);
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High

25637-99-4
Green algae
(Pseudokirchne
riella
subcapitata)
Fresh
72-hour
EC10 = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance (cell
density);
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High
25637-99-4
Green algae
(Pseudokirchne
riella
subcapitata)
Fresh
72-hour
EC50 = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance (cell
density);
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High
25637-99-4
Green algae
(Pseudokirchne
riella
subcapitata)
Fresh
72-hour
EC90 = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance (cell
density);
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High
25637-99-4
Green algae
(Pseudokirchne
riella
subcapitata)
Fresh
96-hour
EC10 = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance (cell
density);
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High
25637-99-4
Green algae
(Pseudokirchne
riella
subcapitata)
Fresh
96-hour
EC50 = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance (cell
density);
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High
4

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Green algae
(P seudokirchne
riella
subcapitata)
Fresh
96-hour
EC90 = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Abundance (cell
density);
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High

25637-99-4
Green algae
(Pseudokirchne
riella
subcapitata)
Fresh
96-hour
NOEC = >0.0037 mg
AI/L
0.0015, 0.0022,
0.0032, 0.0046,
0.0068 mg/L
(nominal); 0.0013,
0.0022,3.38,0.0042,
0.0064 mg/L
(measured, Day 0)
Static, Measured.
Solvent:
Dimethylformamide
Population
growth rate (area
under growth
curve)
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High

25637-99-4
Diatom
(Skeletonema
costatum)
Salt
72-hour
EC50 = 0.0101 mg
AI/L; Seawater, Test
1
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High
1927837
25637-99-4
Diatom
(.Skeletonema
costatum)
Salt
72-hour
EC5o = 0.01 mg AI/L;
Seawater, Test 2
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(.Skeletonema
costatum)
Salt
72-hour
EC50 = 0.0122 mg
AI/L; Rila Marine
Mix, Test 1
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(.Skeletonema
costatum)
Salt
72-hour
EC5o = 0.0118 mg
AI/L; Rila Marine
Mix, Test 2
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(,Skeletonema
costatum)
Salt
72-hour
EC5o = 0.01 mg AI/L;
Instant Ocean sea
salts, Test 1
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(,Skeletonema
costatum)
Salt
72-hour
EC5o = 0.01 mg AI/L;
Instant Ocean sea
salts, Test 2
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(.Skeletonema
costatum)
Salt
72-hour
EC5o = 0.0113 mg
AI/L; Utikem
seawater compound,
Test 1
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(.Skeletonema
costatum)
Salt
72-hour
EC5o = 0.0113 mg
AI/L; Utikem
seawater compound,
Test 2
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
Walsh, 1987,
1927837@@auth
or-year}
High

5

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Diatom
(Skeletonema
costatum)
Salt
72-hour
EC50 = 0.0095 mg
AI/L; HW Marine
Mix, Test 1
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(.Skeletonema
costatum)
Salt
72-hour
EC50 = 0.009 mg
AI/L; HW Marine
Mix, Test 2
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(Thalassiosira
pseudonana)
Salt
72-hour
EC50 = 0.07 mg AI/L;
Seawater, Test 1
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(Thalassiosira
pseudonana)
Salt
72-hour
EC50 = 0.08 mg AI/L;
Seawater, Test 2
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(Thalassiosira
pseudonana)
Salt
72-hour
EC50 = 0.38 mg AI/L;
Rila Marine Mix,
Test 1
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(Thalassiosira
pseudonana)
Salt
72-hour
EC50 = 0.36 mg AI/L;
Rila Marine Mix,
Test 2
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(Thalassiosira
pseudonana)
Salt
72-hour
EC5o = 0.15 mg AI/L;
Instant Ocean sea
salts, Test 1
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(.Thalassiosira
pseudonana)
Salt
72-hour
EC5o = 0.14 mg AI/L;
Instant Ocean sea
salts, Test 2
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(Thalassiosira
pseudonana)
Salt
72-hour
EC50 = 0.1 mg AI/L;
40 fathoms marine
mix, Test 1
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(Thalassiosira
pseudonana)
Salt
72-hour
EC50 = 0.1 mg AI/L;
40 fathoms marine
mix, Test 2
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(Thalassiosira
pseudonana)
Salt
72-hour
EC50 = 0.09 mg AI/L;
Utikem seawater
compound, Test 1
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(Thalassiosira
pseudonana)
Salt
72-hour
EC50 = 0.09 mg AI/L;
Utikem seawater
compound, Test 2
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(Thalassiosira
pseudonana)
Salt
72-hour
EC50 = 0.05 mg AI/L;
HW Marine Mix,
Test 1
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

25637-99-4
Diatom
(Thalassiosira
pseudonana)
Salt
72-hour
EC50 = 0.04 mg AI/L;
HW Marine Mix,
Test 2
Not Reported
Static, Measured,
Solvent: Acetone
Population
change (change in
N/change in time)
{Walsh, 1987,
1927837@@auth
or-year}
High

6

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-50-6
Blue-green
Algae
(Spirulina
subsalsa)
Fresh
168-hour
BCF = 350
0.002 mg/L
Static, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343690@@auth
or-year}
Unacceptable
2343690
134237-50-6
Green Algae
(Scenedesmus
acutus var.
acutus)
Fresh
168-hour
BCF = 407
0.002 mg/L
Static, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343690@@auth
or-year}
Unacceptable

134237-51-7
Blue-green
Algae
{Spirulina
subsalsa)
Fresh
168-hour
BCF = 270
0.002 mg/L
Static, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343690@@auth
or-year}
Unacceptable

134237-51-7
Green Algae
(.Scenedesmus
acutus var.
acutus)
Fresh
168-hour
BCF = 469
0.002 mg/L
Static, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343690@@auth
or-year}
Unacceptable

134237-52-8
Green Algae
(.Scenedesmus
acutus var.
acutus)
Fresh
168-hour
BCF = 390
0.002 mg/L
Static, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343690@@auth
or-year}
Unacceptable

134237-52-8
Blue-green
Algae
{Spirulina
subsalsa)
Fresh
168-hour
BCF = 174
0.002 mg/L
Static, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343690@@auth
or-year}
Unacceptable

Aquatic Invertebrates

3194-55-6
Water flea
{Daphnia
magna)
Fresh
3-hour
ECo = 1000 mg AI/L
0,0.01,0.1,1, 10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High
1928267
3194-55-6
Water flea
{Daphnia
magna)
Fresh
3-hour
EC50 = >1000 mg
AI/L
0, 0, 0.01,0.1, 1, 10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High

3194-55-6
Water flea
{Daphnia
magna)
Fresh
3-hour
EC100 = >1000 mg
AI/L
0, 0,0.01,0.1, 1,10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High

3194-55-6
Water flea
{Daphnia
magna)
Fresh
6-hour
ECo = 1000 mg AI/L
0, 0,0.01,0.1, 1,10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High

3194-55-6
Water flea
{Daphnia
magna)
Fresh
6-hour
EC5o = >1000
0, 0,0.01,0.1, 1,10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High

7

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
3194-55-6
Water flea
(Daphnia
magna)
Fresh
6-hour
ECioo = >1000 mg
AI/L
0, 0,0.01,0.1, 1,10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High

3194-55-6
Water flea
(,Daphnia
magna)
Fresh
24-hour
ECo = 1000 mg AI/L
0, 0,0.01,0.1, 1,10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High
3194-55-6
Water flea
(,Daphnia
magna)
Fresh
24-hour
EC50 = >1000 mg
AI/L
0, 0,0.01,0.1, 1,10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High
3194-55-6
Water flea
(.Daphnia
magna)
Fresh
24-hour
EC100 = >1000 mg
AI/L
0, 0,0.01,0.1, 1, 10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High
3194-55-6
Water flea
(.Daphnia
magna)
Fresh
48-hour
ECo = 1 mg AI/L
,0, 0,0.01,0.1,1,10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High
3194-55-6
Water flea
(.Daphnia
magna)
Fresh
48-hour
EC50 = 146.34 mg
AI/L
0,0.01,0.1,1, 10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High
3194-55-6
Water flea
(.Daphnia
magna)
Fresh
48-hour
EC100 = >1000 mg
AI/L
0, 0,0.01,0.1, 1,10,
100, 1000 mg/L
Static, Nominal
Behavioral:
Swimming
{BASF, 1990,
1928267@@auth
or-year}
High
25637-99-4
Water flea
(.Daphnia
magna)
Fresh
48-hour
No
Mortality/Immobility
Concentration
NR ZERO ~ 0.0032
mg AI/L
0, 0,0.0018,0.0021,
0.0023, 0.0024,
0.0032 mg/L
Flow-through, Measured,
Solvent:
Dimethylformamide
(DMF)
Mortality,
immobility
{Wildlife Intl
LTD, 1997,
1928297@@auth
or-year}
High
1928297
25637-99-4
Water flea
(.Daphnia
magna)
Fresh
48-hour
EC50 = >0.0032 mg
AI/L
0, 0,0.0018,0.0021,
0.0023, 0.0024,
0.0032 mg/L
Flow-through, Measured,
Solvent: DMF
Mortality
{Wildlife Intl
LTD, 1997,
1928297@@auth
or-year}
High
25637-99-4
Water flea
(.Daphnia
magna)
Fresh
48-hour
NOEC = 0.0032 mg
AI/L
0, 0,0.0018,0.0021,
0.0023, 0.0024,
0.0032 mg/L
Flow-through, Measured,
Solvent: DMF
Mortality,
Immobility
{Wildlife Intl
LTD, 1997,
1928297@@auth
or-year}
High
25637-99-4
Water flea
(,Daphnia
magna)
Fresh
24-hour
EC50 = >0.011 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Mortality;
Progeny counts/
numbers
{Wildlife Intl
LTD, 1998,
1928243@@auth
or-year}
High
1928243
25637-99-4
Water flea
(.Daphnia
magna)
Fresh
48-hour
EC50 = >0.011 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Mortality;
Progeny counts/
numbers
{Wildlife Intl
LTD, 1998,
1928243@@auth
or-year}
High
8

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Water flea
(Daphnia
magna)
Fresh
96-hour
EC50 = >0.011 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Mortality;
Progeny counts/
numbers
{Wildlife Intl
LTD, 1998,
1928243@@auth
or-year}
High

25637-99-4
Water flea
(.Daphnia
magna)
Fresh
7-day
EC50 = >0.011 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Mortality;
Progeny counts/
numbers
{Wildlife Intl
LTD, 1998,
1928243@@auth
or-year}
High

25637-99-4
Water flea
(.Daphnia
magna)
Fresh
14-day
EC50 = >0.011 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Mortality;
Progeny counts/
numbers
{Wildlife Intl
LTD, 1998,
1928243@@auth
or-year}
High

25637-99-4
Water flea
(,Daphnia
magna)
Fresh
21-day
EC50 = >0.011 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Mortality;
Progeny counts/
numbers
{Wildlife Intl
LTD, 1998,
1928243@@auth
or-year}
High

25637-99-4
Water flea
(.Daphnia
magna)
Fresh
21-day
NOEC = 0.011 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Mortality
{Wildlife Intl
LTD, 1998,
1928243@@auth
or-year}
High

25637-99-4
Water flea
(.Daphnia
magna)
Fresh
21-day
NOEC = 0.0056 mg
AI/L;
LOEC = 0.011 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Progeny
counts/numbers;
Growth: Weight
{Wildlife Intl
LTD, 1998,
1928243@@auth
or-year}
High

25637-99-4
Water flea
(.Daphnia
magna)
Fresh
21-day
LOEC = 0.0056 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Growth: Length
{Wildlife Intl
LTD, 1998,
1928243@@auth
or-year}
High

25637-99-4
Water flea
(.Daphnia
magna)
Fresh
21-day
NOEC = 0.0031 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Growth: Length
{Wildlife Intl
LTD, 1998,
1928243@@auth
or-year}
High

25637-99-4
Water flea
(.Daphnia
magna)
Fresh
21-day
MATC = 0.0042 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Growth: Length
{Wildlife Intl
LTD, 1998,
1928243@@auth
or-year}
High

25637-99-4
Water flea
(.Daphnia
magna)
Fresh
21-day
NOAEL = 0.0056 mg
AI/L; LOAEL =
0.011 mg AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Reproductive
success (young
per reproductive
day); Growth:
Weight
{Drottar, 1998,
3809169@@auth
or-year}
High
3809169
9

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Water flea
(Daphnia
magna)
Fresh
21-day
NOAEL = 0.0031 mg
AI/L; LOAEL =
0.0056 mg AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Growth: Length
{Drottar, 1998,
3809169@@auth
or-year}
High

25637-99-4
Water flea
(Daphnia
magna)
Fresh
21-day
MATC = 0.0042 mg
AI/L
0, 0,0.00087,0.0016,
0.0031,0.0056,0.011
mg/L
Flow-through, Measured,
Solvent: DMF
Growth: Length
{Drottar, 1998,
3809169@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
1-day
NOAEL = 0.000086
mg AI/L; LOAEL =
0.00086 mg AI/L;
Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: Dimethyl
sulfoxide (DMSO)
7-Ethoxyresorufin
O-deethylase;
Glutathione
(reduced
glutathione);
DNA damage
{Zhang, 2014,
2528343@@auth
or-year}
High
2528343
3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
3-day
LOAEL = 0.000086
mg AI/L; Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
6-day
LOAEL = 0.000086
mg AI/L; Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase;
Glutathione S-
transferase;
Superoxide
dismutase (SOD)
enzyme activity;
Glutathione
(reduced
glutathione);
Lipid
peroxidation
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
10-day
LOAEL = 0.000086
mg AI/L; Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase;
Glutathione S-
transferase;
Superoxide
dismutase (SOD)
enzyme activity;
Glutathione
(reduced
glutathione)
{Zhang, 2014,
2528343@@auth
or-year}
High

10

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
15-day
LOAEL = 0.000086
mg AI/L; Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase;
Glutathione S-
transferase;
Superoxide
dismutase (SOD)
enzyme activity;
Glutathione
(reduced
glutathione)
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
1-day
NOAEL = 0.0086 mg
AI/L; Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
Glutathione S-
transferase;
Superoxide
dismutase (SOD)
enzyme activity
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
3-day
NOAEL = 0.000086
mg AI/L;
LOAEL = 0.00086
mg AI/L; Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
Glutathione S-
transferase;
Superoxide
dismutase (SOD)
enzyme activity;
Glutathione
(reduced
glutathione);
Lipid
peroxidation;
DNA damage
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
6-day
NOAEL = 0.000086
mg AI/L;
LOAEL = 0.00086
mg AI/L; Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
DNA damage
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
10-day
NOAEL = 0.000086
mg AI/L;
LOAEL = 0.00086
mg AI/L; Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
DNA damage;
Lipid
peroxidation
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
15-day
NOAEL = 0.00086
mg AI/L;
LOAEL = 0.0086 mg
AI/L; Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
DNA damage
{Zhang, 2014,
2528343@@auth
or-year}
High

11

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
1-day
NOAEL = 0.00086
mg AI/L;
LOAEL = 0.0086 mg
AI/L; Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
Lipid
peroxidation
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
15-day
NOAEL = 0.000086
mg AI/L;
LOAEL = 0.00086
mg AI/L; Gill tissue
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
Lipid
peroxidation
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
1-day
NOAEL = 0.000086
mg AI/L; LOAEL =
0.00086 mg AI/L ;
Digestive gland
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase;
Glutathione
(reduced
glutathione);
DNA damage
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
3-day
LOAEL = 0.000086
mg AI/L; Digestive
gland
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
6-day
LOAEL = 0.000086
mg AI/L; Digestive
gland
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase;
Glutathione S-
transferase;
Superoxide
dismutase (SOD)
enzyme activity;
Glutathione
(reduced
glutathione);
Lipid
peroxidation
{Zhang, 2014,
2528343@@auth
or-year}
High

12

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
10-day
LOAEL = 0.000086
mg AI/L; Digestive
gland
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase;
Glutathione S-
transferase;
Superoxide
dismutase (SOD)
enzyme activity;
Glutathione
(reduced
glutathione)
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
15-day
LOAEL = 0.000086
mg AI/L; Digestive
gland
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase;
Glutathione S-
transferase;
Superoxide
dismutase (SOD)
enzyme activity;
Glutathione
(reduced
glutathione)
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
1-day
NOAEL = 0.0086 mg
AI/L; Digestive gland
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
Glutathione S-
transferase;
Superoxide
dismutase (SOD)
enzyme activity
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
3-day
NOAEL = 0.000086
mg AI/L; LOAEL =
0.00086 mg AI/L;
Digestive gland
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
Glutathione S-
transferase;
Superoxide
dismutase (SOD)
enzyme activity;
Glutathione
(reduced
glutathione);
DNA damage;
Lipid
peroxidation
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
6-day
NOAEL = 0.000086
mg AI/L;
LOAEL = 0.00086
mg AI/L; Digestive
gland
0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
DNA damage
{Zhang, 2014,
2528343@@auth
or-year}
High

13

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
10-day
NOAEL = 0.000086
mg AI/L;
LOAEL = 0.00086
mg AI/L; Digestive
gland
0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
DNA damage;
Lipid
peroxidation
{Zhang, 2014,
2528343@@auth
or-year}
High

3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
15-day
NOAEL = 0.00086
mg AI/L;
LOAEL = 0.0086 mg
AI/L; Digestive gland
0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
DNA damage
{Zhang, 2014,
2528343@@auth
or-year}
High
3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
1-day
NOAEL = 0.00086
mg AI/L;
LOAEL = 0.0086 mg
AI/L; Digestive gland
0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
Lipid
peroxidation
{Zhang, 2014,
2528343@@auth
or-year}
High
3194-55-6
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
15-day
NOAEL = 0.000086
mg AI/L;
LOAEL = 0.00086
mg AI/L; Digestive
gland
0.000086, 0.00086,
0.0086 mg/L
Renewal, Not reported,
Solvent: DMSO
Lipid
peroxidation
{Zhang, 2014,
2528343@@auth
or-year}
High
25637-99-4
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
3-day
LOAEL = 0.000086
mg AI/L
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Nominal,
Solvent: DMSO
Ferritin mRNA;
Catalase mRNA;
Dihydrodiol
dehydrogenase
mRNA;
Cytochrome c
oxidase subunit 1
mRNA; NADH:
ubiquinone
reductase (H(+)-
translocating)
mRNA
{Zhang, 2014,
2528343@@auth
or-year}
Unacceptable
1928024
14

-------
PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
10-day
LOAEL = 0.000086
mg AI/L
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Nominal,
Solvent: DMSO
Ferritin mRNA;
Catalase mRNA;
Dihydrodiol
dehydrogenase
mRNA; C-type
Lectin like
mRNA;
Elongation factor-
1 alpha mRNA;
Hemocyanin
subunit 2 mRNA
{Zhang, 2014,
2528343@@auth
or-year}
Unacceptable

25637-99-4
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
10-day
NOAEL = 0.0086 mg
AI/L
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Nominal,
Solvent: DMSO
Cytochrome c
oxidase subunit 1
mRNA
{Zhang, 2014,
2528343@@auth
or-year}
Unacceptable

25637-99-4
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
10-day
NOAEL = 0.000086
mg AI/L;
LOAEL = 0.00086
mg AI/L
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Nominal,
Solvent: DMSO
NADH:
ubiquinone
reductase (H(+)-
translocating)
mRNA
{Zhang, 2014,
2528343@@auth
or-year}
Unacceptable

25637-99-4
Japanese
Littleneck
Clam
(Venerupis
philippinarum)
Salt
10-day
NOAEL = 0.00086
mg AI/L;
LOAEL = 0.0086 mg
AI/L
0, 0.000086, 0.00086,
0.0086 mg/L
Renewal, Nominal,
Solvent: DMSO
Purine nucleoside
phosphorylase
mRNA
{Zhang, 2014,
2528343@@auth
or-year}
Unacceptable

25637-99-4
Sea urchin
(Psammechinus
miliaris)
Salt
72 hours
post
fertilization
NOAEL=
0.06416989 mg AI/L;
Exp. A
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal
{Anselmo, 2011,
1274149@@auth
or-year}
High
1274149
25637-99-4
Sea urchin
(.Psammechinus
miliaris)
Salt
72 hours
post
fertilization
NOAEL = 0.032 mg
AI/L; LOAEL =
0.064 mg AI/L; Exp.
B
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal
{Anselmo, 2011,
1274149@@auth
or-year}
High

25637-99-4
Sea urchin
(.Psammechinus
miliaris)
Salt
2-4 days
post
fertilization
NOAEL = 0.032 mg
AI/L; LOAEL =
0.064 mg AI/L; Exp.
A
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal
{Anselmo, 2011,
1274149@@auth
or-year}
High

25637-99-4
Sea urchin
(,Psammechinus
miliaris)
Salt
2-4 days
post
fertilization
NOAEL = 0.032 mg
AI/L; LOAEL =
0.064 mg AI/L; Exp.
B
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal
{Anselmo, 2011,
1274149@@auth
or-year}
High

15

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Sea urchin
(.Psammechinus
miliaris)
Salt
2-4 days
post
fertilization
ECso = 0.0453 mg
AI/L; Exp. B
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal
{Anselmo, 2011,
1274149@@auth
or-year}
High

25637-99-4
Sea urchin
(.Psammechinus
miliaris)
Salt
7-9 days
post
fertilization
NOAEL = 0.0320 mg
AI/L; LOAEL =
0.064 mg AI/L; Exp.
B
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal
{Anselmo, 2011,
1274149@@auth
or-year}
High

25637-99-4
Sea urchin
(,Psammechinus
miliaris)
Salt
7-9 days
post
fertilization
ECso = 0.040407 mg
AI/L; Exp. B
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal
{Anselmo, 2011,
1274149@@auth
or-year}
High

25637-99-4
Sea urchin
(.Psammechinus
miliaris)
Salt
14-16 days
post
fertilization
NOAEL = 0.0325 mg
AI/L; LOAEL =
0.064 mg AI/L; Exp.
A
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal
{Anselmo, 2011,
1274149@@auth
or-year}
High

25637-99-4
Sea urchin
(.Psammechinus
miliaris)
Salt
14-16 days
post
fertilization
EC50 =
0.056 mg AI/L; Exp.
A
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal
{Anselmo, 2011,
1274149@@auth
or-year}
High

25637-99-4
Sea urchin
(.Psammechinus
miliaris)
Salt
14-16 days
post
fertilization
NOAEL = 0.0325 mg
AI/L; LOAEL =
0.064 mg AI/L; Exp.
B
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal
{Anselmo, 2011,
1274149@@auth
or-year}
High

25637-99-4
Sea urchin
(,Psammechinus
miliaris)
Salt
14-16 days
post
fertilization
EC50 = 0.035mg
AI/L; Exp. B
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal
{Anselmo, 2011,
1274149@@auth
or-year}
High

25637-99-4
Sea urchin
(.Psammechinus
miliaris)
Salt
16 days
post
fertilization
NOAEL = 0.032 mg
AI/L; LOAEL =
0.064 mg AI/L; Exp.
A
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth: Develop-
ment
{Anselmo, 2011,
1274149@@auth
or-year}
High

25637-99-4
Sea urchin
(.Psammechinus
miliaris)
Salt
16 days
post
fertilization
NOAEL = 0.0058 mg
AI/L;
LOAEL = 0.016 mg
AI/L Exp. B
0, 0.006,0.016,
0.032, 0.064 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth: Develop-
ment
{Anselmo, 2011,
1274149@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
96-hour
NOEC = 0.8 mg
AI/L;
LOEC = >0.8 mg
AI/L
0, 0,0.08,0.3,0.8
mg/L
Renewal, Nominal,
Solvent: DMSO
Mortality
{Shi, 2017,
3546057@@auth
or-year}
High
3546057
25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
96-hour
Kinetic BCF =
87,300
0, 0.002 mg/L
Renewal, Nominal
Residue;
bioconcentration
{Shi, 2017,
3546057@@auth
or-year}
High

16

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
96-hour
Steady-State BCF =
63,400
0, 0.002 mg/L
Renewal, Nominal
Residue;
bioconcentration
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
96-hour
Lipid-Normalized
Kinetic BCF =
78,400
0, 0.002 mg/L
Renewal, Nominal
Residue;
bioconcentration
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
3-day
NOAEL = 0.8 mg
AI/L
0, 0, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Glutathione S-
transferase
mRNA; p53
mRNA
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(.Tigriopus
japonicus)
Salt
7-day
NOAEL = 0.8 mg
AI/L
0, 0, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Glutathione S-
transferase
mRNA; Catalase;
p53 mRNA;
Superoxide
dismutase mRNA
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(.Tigriopus
japonicus)
Salt
14-day
NOAEL = 0.3 mg
AI/L;
LOAEL = 0.8 mg
AI/L
0, 0, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Glutathione S-
transferase
mRNA; 8-
oxoguanine DNA
glycosylase
mRNA; p53
mRNA
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(.Tigriopus
japonicus)
Salt
3-day
NOAEL = 0.3 mg
AI/L;
LOAEL = 0.8 mg
AI/L
0, 0, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Catalase; 8-
oxoguanine DNA
glycosylase
mRNA;
Superoxide
dismutase mRNA
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
14-day
LOAEL = 0.3 mg
AI/L
0, 0, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Catalase;
Caspase-3 mRNA
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(.Tigriopus
japonicus)
Salt
7-day
NOAEL = 0.3 mg
AI/L;
LOAEL = 0.8 mg
AI/L
0, 0, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
8-oxoguanine
DNA glycosylase
mRNA
{Shi, 2017,
3546057@@auth
or-year}
High

17

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
3-day
LOAEL = 0.3 mg
AI/L
0, 0, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Caspase-3 mRNA
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
7-day
LOAEL = 0.3 mg
AI/L
0, 0, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Caspase-3 mRNA
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
10-day
NOAEL = 0.8 mg
AI/L; F0 generation
0, 0, 0.008, 0.03,
0.08, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Reproductive:
Fecundity;
Progeny
counts/numbers;
Sex ratio
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
10-day
NOAEL = 0.8 mg
AI/L; F1 generation
0, 0, 0.008, 0.03,
0.08, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Reproductive:
Fecundity;
Progeny
counts/numbers;
Sex ratio
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(.Tigriopus
japonicus)
Salt
20-day
NOEC = 0.08 mg
AI/L;
LOEC = 0.3 mg
AI/L; F0 generation;
maturation period
0, 0, 0.008, 0.03,
0.08, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth,
Developmental
stage
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
20-day
NOEC = 0.008 mg
AI/L;
LOEC = 0.03 mg
AI/L; F1 generation;
maturation period
0, 0, 0.008, 0.03,
0.08, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth,
Developmental
stage
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(.Tigriopus
japonicus)
Salt
20-day
NOEC = 0.008 mg
AI/L;
LOEC = 0.03 mg
AI/L; F0 generation;
nauplius phase
0, 0, 0.008, 0.03,
0.08, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth,
Developmental
stage
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(Tigriopus
japonicus)
Salt
20-day
LOEC = 0.008 mg
AI/L; F1 generation;
nauplius phase
0, 0, 0.008, 0.03,
0.08, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Growth,
Developmental
stage
{Shi, 2017,
3546057@@auth
or-year}
High

25637-99-4
Harpacticoid
Copepod
(.Tigriopus
japonicus)
Salt
40-day
NOEC = 0.8 mg AI/L
0, 0, 0.008, 0.03,
0.08, 0.3, 0.8 mg/L
Renewal, Nominal,
Solvent: DMSO
Mortality
{Shi, 2017,
3546057@@auth
or-year}
High

18

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Balic Macoma
Or Clam
(Macoma
balthica)
Salt
50-day
LOAEL = 0.1 mg/L
0, 0.1, 0.25 mg/L
Multiple routes, Nominal
Nuclear
abnormality:
micronuclei;
frequency of dead
cells; Mean
number of
nucleoli;
binucleated cells,
formation of
nucleoplasmic
bridges, nuclear
buds, occurrence
of pleomorphic
and hypertrophic
cells
{Smolarz, 2009,
1927697@@auth
or-year}
High
1927697
25637-99-4
Amphipod
(.Hyalella
azteca)
Fresh
28-day
NOEC > 1000 mg/kg
dwt sediment
31,63, 125,250, 500
and 1,000 mg/kg dwt
sediment (Nominal
concentrations)
Flow-through, Measured,
Solvent: DMF range-
finding study conducted
in the presence of 2 %
TOC. Further study
details were not provided.
reduced
survivability
{Thomas, 2003,
4269889@@auth
or-year}
High
4269889
25637-99-4
Amphipod
(.Hyalella
azteca)
Fresh
28-day
LOEC = 1000 mg/kg
dwt sediment
31,63, 125,250, 500
and 1,000 mg/kg dwt
sediment (Nominal
concentrations)
Flow-through, Measured,
Solvent: DMF range-
finding study conducted
in the presence of 2 %
TOC. Further study
details were not provided.
reduced
survivability
{Thomas, 2003,
4269889@@auth
or-year}
High

25637-99-4
Amphipod
(.Hyalella
azteca)
Fresh
28-day
NOEC = 1000 mg/kg
dwt sediment
31,63, 125,250, 500
and 1,000 mg/kg dwt
sediment (Nominal
concentrations)
Flow-through, Measured,
Solvent: DMF range-
finding study conducted
in the presence of 5%
TOC. Further study
details were not provided.
reduced
survivability
{Thomas, 2003,
4269912@@auth
or-year}
High
4269912
25637-99-4
Amphipod
{Hyalella
azteca)
Fresh
28-day
NOEC = 1000 mg/kg
dwt sediment
31,63, 125,250, 500
and 1,000 mg/kg dwt
sediment (Nominal
concentrations)
Flow-through, Measured,
Solvent: DMF range-
finding study conducted
in the presence of 5%
TOC. Further study
details were not provided.
reduced
survivability
{Thomas, 2003,
4269912@@auth
or-year}
High

19

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.

Worm
(Lumbriculus
variegatus)
Fresh
28-day
NOEC = 3.1 mg/kg
dry weight sediment
0.05, 0.5, 5, 50, and
500 mg/kg dry weight
sediment, measured
concentrations were
ND, 0.2, 3.1,28.7,
and 303.2 mg/kg dry
weight.
28-day static test using
dechlorinated tap water
measured concentrations-
0,nd3, 0.25, 3.25, 29.25
and 311.35 mg/kg
sediment dw;
40 worms per treatment;
artificial sediment: 1.8%
organic carbon, grain
size 100-2000 jxm;
Total number of
worms
{Oetken, 2001,
3809143@@autho
r-year}
High
3809143

Worm
(Lumbriculus
variegatus)
Fresh
28-day
LOEC = 28.7 mg/kg
dry weight sediment
0.05, 0.5, 5, 50, and
500 mg/kg dry weight
sediment, measured
concentrations were
ND, 0.2, 3.1,28.7,
and 303.2 mg/kg dry
weight.
28-day static test using
dechlorinated tap water
measured concentrations-
0,nd3, 0.25, 3.25, 29.25
and 311.35 mg/kg
sediment dw;
40 worms per treatment;
artificial sediment: 1.8%
organic carbon, grain
size 100-2000 |im;
Total number of
worms
{Oetken, 2001,
3809143@@autho
r-year}
High


Worm
(Lumbriculus
variegatus)
Fresh
28-day
NOEC = 28.7 mg/kg
dry weight sediment
0.05, 0.5, 5, 50, and
500 mg/kg dry weight
sediment, measured
concentrations were
ND, 0.2, 3.1,28.7,
and 303.2 mg/kg dry
weight.
28-day static test using
dechlorinated tap water
measured concentrations-
0,nd3, 0.25, 3.25, 29.25
and 311.35 mg/kg
sediment dw;
40 worms per treatment;
artificial sediment: 1.8%
organic carbon, grain
size 100-2000 |im;
Large vs small
worms
{Oetken, 2001,
3809143@@autho
r-year}
High


Worm
(Lumbriculus
variegatus)
Fresh
28-day
NOEC = 3.1 mg/kg
dry weight sediment
0.05, 0.5, 5, 50, and
500 mg/kg dry weight
sediment, measured
concentrations were
ND, 0.2, 3.1,28.7,
and 303.2 mg/kg dry
weight.
28-day static test using
dechlorinated tap water
measured concentrations-
0,nd3, 0.25, 3.25, 29.25
and 311.35 mg/kg
sediment dw;
40 worms per treatment;
artificial sediment: 1.8%
organic carbon, grain
size 100-2000 |xm;
Large vs small
worms
{Oetken, 2001,
3809143@@autho
r-year}
High

Aquatic Vertebrates

20

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
1837-91-8
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
2-hour
NOAEL = 27.877 mg
AI/L
0, 27.877 mg/L
Ln vitro, Nominal
Insulin-like
growth factor 1
mRNA; Signal
Transducer and
Activator of
Transcription
protein 5
{Reindl, 2011,
3586425@@auth
or-year}
High
3586425
25637-99-4
Bluegill
(Lepomis
macrochirus)
Fresh
24-hour
LC50 = >100 mg AI/L
0, 0, 10.0, 18.0, 32.0,
56.0,100.0 mg/L
Static, Nominal, Solvent:
Acetone
Mortality
{Great Lakes
Chem Corp,
1994,
1928289@@auth
or-year}
High
1928289
25637-99-4
Bluegill
(,Lepomis
macrochirus)
Fresh
48-hour
LC50 = >100 mg AI/L
0, 0, 10.0, 18.0, 32.0,
56.0,100.0 mg/L
Static, Nominal, Solvent:
Acetone
Mortality
{Great Lakes
Chem Corp,
1994,
1928289@@auth
or-year}
High
25637-99-4
Bluegill
(.Lepomis
macrochirus)
Fresh
96-hour
LC50 = >100 mg AI/L
0, 0, 10.0, 18.0, 32.0,
56.0,100.0 mg/L
Static, Nominal, Solvent:
Acetone
Mortality
{Great Lakes
Chem Corp,
1994,
1928289@@auth
or-year}
High
25637-99-4
Bluegill
(,Lepomis
macrochirus)
Fresh
96-hour
NOEC = >100 mg
AI/L
0, 0, 10.0, 18.0, 32.0,
56.0,100.0 mg/L
Static, Nominal, Solvent:
Acetone
Mortality
{Great Lakes
Chem Corp,
1994,
1928289@@auth
or-year}
High
25637-99-4
Bluegill
(.Lepomis
macrochirus)
Fresh
96-hour
NR-ZERO = >100
mg AI/L
0, 0, 10.0, 18.0, 32.0,
56.0,100.0 mg/L
Static, Nominal, Solvent:
Acetone
Abnormal
behavior
{Great Lakes
Chem Corp,
1994,
1928289@@auth
or-year}
High
3194-55-6
Zebrafish
(Danio rerio)
Fresh
72-hour
NOAEL = 32 mg
AI/L
0, 32 mg/L
Static, Nominal
Thyroxine
{Thienpont, 2011,
1062065@@auth
or-year}
Low
1062065
25637-99-4
Bluegill
(.Lepomis
macrochirus)
Fresh
24-hour
LC50 = >100 mg AI/L
0, 0, 10.0, 18.0, 32.0,
56.0,100.0 mg/L
Static, Nominal
Mortality
{Great Lakes
Chem Corp,
1994,
1928289@@auth
or-year}
Unacceptable
1928239
21

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Bluegill
(Lepomis
macrochirus)
Fresh
48-hour
LC50 = >100 mg AI/L
0, 0, 10.0, 18.0, 32.0,
56.0,100.0 mg/L
Static, Nominal
Mortality
{Great Lakes
Chem Corp,
1994,
1928289@@auth
or-year}
Unacceptable

25637-99-4
Bluegill
(,Lepomis
macrochirus)
Fresh
96-hour
LC50 = >100 mg AI/L
0, 0, 10.0, 18.0, 32.0,
56.0,100.0 mg/L
Static, Nominal
Mortality
{Great Lakes
Chem Corp,
1994,
1928289@@auth
or-year}
Unacceptable

25637-99-4
Bluegill
(.Lepomis
macrochirus)
Fresh
96-hour
NOEC = 100 mg
AI/L
0, 0, 10.0, 18.0, 32.0,
56.0,100.0 mg/L
Static, Nominal
Abnormal
behavior
{Great Lakes
Chem Corp,
1994,
1928289@@auth
or-year}
Unacceptable

25637-99-4
Bluegill
(,Lepomis
macrochirus)
Fresh
96-hour
NR-ZERO = 100 mg
AI/L
0, 0, 10.0, 18.0, 32.0,
56.0,100.0 mg/L
Static, Nominal
Mortality
{Great Lakes
Chem Corp,
1994,
1928289@@auth
or-year}
Unacceptable

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
24-hour
LC50 = >0.0025 mg
AI/L
0, 0,0.00075,0.0015,
0.0023, 0.0023,
0.0025 mg/L
Flow-through, Measured.
Solvent: DMF
Mortality
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High
3586422
! 928298
25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
48-hour
LC50 = >0.0025 mg
AI/L
0, 0,0.00075,0.0015,
0.0023, 0.0023,
0.0025 mg/L
Flow-through, Measured,
Solvent: DMF
Mortality
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
72-hour
LC50 = >0.0025 mg
AI/L
0, 0,0.00075,0.0015,
0.0023, 0.0023,
0.0025 mg/L
Flow-through, Measured,
Solvent: DMF
Mortality
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
96-hour
LC50 = >0.0025 mg
AI/L
0, 0,0.00075,0.0015,
0.0023, 0.0023,
0.0025 mg/L
Flow-through, Measured,
Solvent: DMF
Mortality
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
96-hour
NOEC = 0.0025 mg
AI/L
0, 0,0.00075,0.0015,
0.0023, 0.0023,
0.0025 mg/L
Flow-through, Measured
Mortality
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High

22

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
96-hour
NR-ZERO = >0.0025
mg AI/L
0, 0,0.00075,0.0015,
0.0023, 0.0023,
0.0025 mg/L
Flow-through, Measured,
Solvent: DMF
Mortality
{Wildlife Intl
LTD, 1997,
1928298@@auth
or-year}
High

3194-55-6
Zebrafish
Not
47-hour
NOAEL = 0.128 mg
0,0.001,0.013,0.128
Renewal, Nominal,
Hatching rate
{Wu, 2013,
High
1927533

(Danio rerio)
reported

AI/L
mg/L
Solvent: DMSO

1927533@@auth
or-year}


3194-55-6
Zebrafish
Not
47-hour
NOAEL = 0.013 mg
0,0.001,0.013,0.128
Renewal, Nominal,
Heart rate
{Wu, 2013,
High


(Danio rerio)
reported

AI/L; LOAEL =
0.128 mg AI/L
mg/L
Solvent: DMSO

1927533@@auth
or-year}


3194-55-6
Zebrafish
Not
59-hour
LOAEL = 0.001 mg
0,0.001,0.013,0.128
Renewal, Nominal,
Heart rate
{Wu, 2013,
High


{Danio rerio)
reported

AI/L
mg/L
Solvent: DMSO

1927533@@auth
or-year}


3194-55-6
Zebrafish
Not
71-hour
LOAEL = 0.001 mg
0,0.001,0.013,0.128
Renewal, Nominal,
Heart rate; T-box
{Wu, 2013,
High


{Danio rerio)
reported

AI/L
mg/L
Solvent: DMSO
5amRNA;
Homeobox
protein Nkx-2.5
mRNA
1927533@@auth
or-year}


3194-55-6
Zebrafish
Not
71-hour
NOAEL = 0.001 mg
0,0.001,0.013,0.128
Renewal, Nominal,
Cardiac
{Wu, 2013,
High


{Danio rerio)
reported

AI/L;
LOAEL = 0.013 mg
AI/L
mg/L
Solvent: DMSO
arrhythmia
1927533@@auth
or-year}


23

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
3194-55-6
Zebrafish
(Danio rerio)
Not
reported
71-hour
NOAEL = 0.128 mg
AI/L
0,0.001,0.013,0.128
mg/L
Renewal, Nominal,
Solvent: DMSO
ATPase, Ca++
transporting,
cardiac muscle,
slow twitch 2a
mRNA; Troponin
T type 2a
(cardiac) mRNA;
myH6 expression;
End-diastolic
Volume; Stroke
volume; Caspase
3; Actin, alpha,
cardiac muscle la
mRNA; Myosin,
heavy chain 6,
cardiac muscle,
alpha mRNA;
Cardiac output;
End-systolic
Volume;
Mortality;
Abnormal; whole
malformation rate
{Wu, 2013,
1927533@@auth
or-year}
High

3194-55-6
Zebrafish
{Danio rerio)
Not
reported
71-hour
NOAEL = 0.013 mg
AI/L;
LOAEL = 0.128 mg
AI/L
0,0.001,0.013,0.128
mg/L
Renewal, Nominal,
Solvent: DMSO
ATPase, Ca++
transporting,
cardiac muscle,
slow twitch 2b
mRNA;
Ryanodine
receptor 2a
(cardiac) mRNA
{Wu, 2013,
1927533@@auth
or-year}
High
134237-52-8
Zebrafish
(Danio rerio)
Fresh
44-hour
NOEC = 0.01 mg
AI/L;
LOEC = 0.1 mg AI/L
0, 0.01,0.1, 1.0 mg/L
Renewal, Nominal,
Solvent: DMSO
Heart rate
{Du, 2012,
1927610@@auth
or-year}
High
1927610
134237-52-8
Zebrafish
(Danio rerio)
Fresh
68-hour
LOEC = 0.01 mg
AI/L
0, 0.01,0.1, 1.0 mg/L
Renewal, Nominal,
Solvent: DMSO
Hatching success
{Du, 2012,
1927610@@auth
or-year}
High
134237-52-8
Zebrafish
{Danio rerio)
Fresh
92-hour
NOEC = 0.1 mg
AI/L;
LOEC = 1 mg AI/L
0, 0.01,0.1, 1.0 mg/L
Renewal, Nominal,
Solvent: DMSO
Heart rate
{Du, 2012,
1927610@@auth
or-year}
High
24

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-52-8
Zebrafish
(Danio rerio)
Fresh
92-hour
NOEC = 0.01 mg
AI/L;
LOEC = 0.1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Growth: Length
{Du, 2012,
1927610@@auth
or-year}
High

134237-52-8
Zebrafish
{Danio rerio)
Fresh
92-hour
LOEC = 0.01 mg
AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Mortality
{Du, 2012,
1927610@@auth
or-year}
High
134237-52-8
Zebrafish
{Danio rerio)
Fresh
116-hour
LOEC = 0.01 mg
AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal.
Malformation rate
{Du, 2012,
1927610@@auth
or-year}
High
134237-52-8
Zebrafish
{Danio rerio)
Fresh
116-hour
NOEC = 0.01 mg
AI/L;
LOEC = 0.1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Reactive oxygen
species; Caspase
3; Caspase 9
{Du, 2012,
1927610@@auth
or-year}
High
134237-51-7
Zebrafish
{Danio rerio)
Fresh
44-hour
NOEC = 0.01 mg
AI/L;
LOEC = 0.1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Heart rate
{Du, 2012,
1927610@@auth
or-year}
High
134237-51-7
Zebrafish
{Danio rerio)
Fresh
68-hour
NOEC = 0.01 mg
AI/L;
LOEC = 0.1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Hatching success
{Du, 2012,
1927610@@auth
or-year}
High
134237-51-7
Zebrafish
{Danio rerio)
Fresh
92-hour
NOEC = 0.01 mg
AI/L;
LOEC = 0.1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Growth: Length;
Heart rate;
Mortality
{Du, 2012,
1927610@@auth
or-year}
High
134237-51-7
Zebrafish
{Danio rerio)
Fresh
116-hour
LOEC = 0.01 mg
AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal.
Malformation rate
{Du, 2012,
1927610@@auth
or-year}
High
134237-51-7
Zebrafish
{Danio rerio)
Fresh
116-hour
NOEC = 0.01 mg
AI/L;
LOEC = 0.1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Reactive oxygen
species
{Du, 2012,
1927610@@auth
or-year}
High
134237-51-7
Zebrafish
{Danio rerio)
Fresh
116-hour
NOEC = 0.1 mg
AI/L;
LOEC = 1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Caspase 3;
Caspase 9
{Du, 2012,
1927610@@auth
or-year}
High
134237-50-6
Zebrafish
{Danio rerio)
Fresh
44-hour
NOEC = 0.1 mg
AI/L;
LOEC = 1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Heart rate
{Du, 2012,
1927610@@auth
or-year}
High
134237-50-6
Zebrafish
{Danio rerio)
Fresh
68-hour
NOEC = 0.01 mg
AI/L;
LOEC = 0.1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Hatching success
{Du, 2012,
1927610@@auth
or-year}
High
134237-50-6
Zebrafish
{Danio rerio)
Fresh
92-hour
NOEC = 0.01 mg
AI/L;
LOEC = 0.1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Heart rate
{Du, 2012,
1927610@@auth
or-year}
High
134237-50-6
Zebrafish
{Danio rerio)
Fresh
92-hour
NOEC = 0.1 mg
AI/L;
LOEC = 1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Growth: Length;
Mortality
{Du, 2012,
1927610@@auth
or-year}
High
25

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CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-50-6
Zebrafish
(Danio rerio)
Fresh
116-hour
NOEC = 0.01 mg
AI/L;
LOEC = 0.1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal.
Malformation rate
{Du, 2012,
1927610@@auth
or-year}
High

134237-50-6
Zebrafish
{Danio rerio)
Fresh
116-hour
NOEC = 0.1 mg
AI/L;
LOEC = 1 mg AI/L
0, 0.01,0.1, l.Omg/L
Renewal, Nominal,
Solvent: DMSO
Reactive oxygen
species; Caspase
3; Caspase 9
{Du, 2012,
1927610@@auth
or-year}
High
3194-55-6
Zebrafish
{Danio rerio)
Fresh
92-hour
LOAEL = 0.05 mg
AI/L
0,0.05,0.1,0.5,1.0
mg/L
Aquatic-not reported,
Nominal, Solvent:
DMSO
Heart rate; bax
mRNA; Mortality
{Deng, 2009,
1927716@@auth
or-year}
High
1927716
3194-55-6
Zebrafish
{Danio rerio)
Fresh
92-hour
NOAEL = 0.05 mg
AI/L;
LOAEL = 0.1 mg
AI/L
0,0.05,0.1,0.5,1.0
mg/L
Aquatic-not reported,
Nominal, Solvent:
DMSO
Growth: Length;
Reactive oxygen
species; Caspase-
3 mRNA
expression
profile; Caspase-9
mRNA
expression
profile; Growth:
Abnormal
malformation rate
{Deng, 2009,
1927716@@auth
or-year}
High
3194-55-6
Zebrafish
{Danio rerio)
Fresh
92-hour
NOAEL = 0.5 mg
AI/L;
LOAEL = 1 mg AI/L
0,0.05,0.1,0.5,1.0
mg/L
Aquatic-not reported,
Nominal, Solvent:
DMSO
p53 mRNA; E3
ubiquitin-protein
ligase; Bcl-2-
binding
component 3
mRNA; Bcl-2
mRNA;
Apoptotic
protease-
activating factor 1
mRNA; Caspase-
3 mRNA
induction;
Caspase-9 mRNA
induction
{Deng, 2009,
1927716@@auth
or-year}
High
25637-99-4
Zebrafish
{Danio rerio)
Fresh
96-hour
LOAEL = 0.002 mg
AI/L
0, 0.002,0.01,0.1,
0.5,2.5,10 mg/L
Renewal, Nominal,
Solvent: DMSO
Hatch delay
{Hu, 2009,
1927732@@auth
or-year}
High
1927732
25637-99-4
Zebrafish
{Danio rerio)
Fresh
96-hour
NOAEL = 10 mg
AI/L
0, 0.002,0.01,0.1,
0.5,2.5,10 mg/L
Renewal, Nominal,
Solvent: DMSO
Mortality
{Hu, 2009,
1927732@@auth
or-year}
High
26

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CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Zebrafish
(Danio rerio)
Fresh
96-hour
NOAEL = 0.1 mg
AI/L;
LOAEL = 0.5 mg
AI/L
0, 0.002,0.01,0.1,
0.5,2.5, lOmg/L
Renewal, Nominal,
Solvent: DMSO
Malondialdehyde
{Hu, 2009,
1927732@@auth
or-year}
High

25637-99-4
Zebrafish
(Danio rerio)
Fresh
96-hour
NOAEL = 0.002 mg
AI/L;
LOAEL = 0.01 mg
AI/L
0, 0.002,0.01,0.1,
0.5,2.5, lOmg/L
Renewal, Nominal,
Solvent: DMSO
Heat shock
protein 70
{Hu, 2009,
1927732@@auth
or-year}
High
25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
5-day
NR-ZERO =
0.000017- 0.0003 mg
AI/L
0, 0,0.000017-
0.0003 mg/L
Flow-through, Measured,
Solvent: Methanol
Mortality
{Lower, 2008,
3618094@@auth
or-year}
High
3618094
25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
5-day
NOAEL = 0.00005
mg AI/L; Exp. 1
0, 0.000005, 0.00005
mg/L
Flow-through, Nominal,
Solvent: Methanol
Growth: Length;
Growth: Weight;
Gonado somatic
index
{Lower, 2008,
3618094@@auth
or-year}
High
25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
5-day
NOAEL = 0.00005
mg AI/L; Exp. 2
0, 0, 0.000005,
0.00005 mg/L
Flow-through, Nominal,
Solvent: Methanol
Growth: Length;
Growth: Weight;
Gonado somatic
index
{Lower, 2008,
3618094@@auth
or-year}
High
25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
5-day
NOAEL = 0.000017-
0.0003 mg AI/L
0, 0,0.000017-
0.0003 mg/L
Flow-through, Measured,
Solvent: Methanol
Condition index:
5-day freshwater
dosing period, 3-
day transfer to
salt water;
Sodium
potassium
ATPase; 5-day
freshwater dosing
period, 3-day
transfer to salt
water; Thyroxine;
5-day freshwater
dosing period, 3-
day transfer to
salt water;
Triiodothyronine;
5-day freshwater
dosing period, 3-
day transfer to
salt water
{Lower, 2008,
3618094@@auth
or-year}
High
27

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CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
5-day
NOAEL = 0.00005
mg AI/L
0, 0.000005, 0.00005
mg/L
Flow-through, Nominal,
Solvent: Methanol
Trans-epithelial
voltage gradient;
Accessory
reproductive
fluid;
Testosterone
{Lower, 2008,
3618094@@auth
or-year}
High

25637-99-4
Atlantic
Fresh
5-day
LOAEL = 0.000005
0, 0, 0.000005,
Flow-through, Nominal,
11-
{Lower, 2008,
High


salmon (Salmo
salar)


mg AI/L
0.00005 mg/L
Solvent: Methanol
Ketotestosterone;
17,20beta-
Dihydroxy-4-
pregnen-3-one
3618094@@auth
or-year}


25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
30-day
NOEC = 0.000011
0, 0.000011 mg/L
Flow-through, Measured,
Solvent: Methanol
Mortality,
Growth: length;
Condition Factor
{Lower, 2008,
3618094@@auth
or-year}
High

25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
30-day
LOEC = 0.000011
0, 0.000011 mg/L
Flow-through, Measured,
Solvent: Methanol
Gill Na+/K+
ATPase activity;
Plasma T4
{Lower, 2008,
3618094@@auth
or-year}
High

25637-99-4
Chinese Rare
Minnow
(Gobiocypris
rams)
Fresh
14-day
NOAEL = 0.5 mg
AI/L
0, 0.001,0.01,0.1,
0.5 mg/L
Renewal, Nominal,
Solvent: DMSO
Pentylresorufin
O-deethylase;
Reactive oxygen
species; 7-
Ethoxyresorufin
O-deethylase;
Thiobarbituric
acid reactive
substances;
Protein carbonyls;
DNA damage;
Superoxide
dismutase (SOD)
enzyme activity;
Glutathione
disulfide
(oxidized
glutathione)
{Zhang, 2008,
1927768@@auth
or-year}
High

25637-99-4
Chinese Rare
Minnow
(Gobiocypris
rants)
Fresh
28-day
NOAEL = 0.1 mg
AI/L;
LOAEL = 0.5 mg
AI/L
0, 0.001,0.01,0.1,
0.5 mg/L
Renewal, Nominal,
Solvent: DMSO
Pentylresorufin
O-deethylase; 7-
Ethoxyresorufin
O-deethylase;
Superoxide
dismutase (SOD)
enzyme activity
{Zhang, 2008,
1927768@@auth
or-year}
High
1927768
28

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Chinese Rare
Minnow
(Gobiocypris
rants)
Fresh
42-day
NOAEL = 0.01 mg
AI/L;
LOAEL = 0.1 mg
AI/L
0, 0.001,0.01,0.1,
0.5 mg/L
Renewal, Measured,
Solvent: DMSO
Pentylresorufin
O-deethylase; 7-
Ethoxyresorufm
O-deethylase;
Thiobarbituric
acid reactive
substances
{Zhang, 2008,
1927768@@auth
or-year}
High

25637-99-4
Chinese Rare
Minnow
(Gobiocypris
rants)
Fresh
28-day
NOAEL = 0.01 mg
AI/L;
LOAEL = 0.1 mg
AI/L
0, 0.001,0.01,0.1,
0.5 mg/L
Renewal, Nominal,
Solvent: DMSO
Reactive oxygen
species; Protein
carbonyls; DNA
damage;
Glutathione
disulfide
(oxidized
glutathione)
{Zhang, 2008,
1927768@@auth
or-year}
High

25637-99-4
Chinese Rare
Minnow
(Gobiocypris
rants)
Fresh
42-day
NOAEL = 0.001 mg
AI/L;
LOAEL = 0.01 mg
AI/L
0, 0.001,0.01,0.1,
0.5 mg/L
Renewal, Nominal,
Solvent: DMSO
Reactive oxygen
species; Protein
carbonyls; DNA
damage;
Superoxide
dismutase (SOD)
enzyme activity
{Zhang, 2008,
1927768@@auth
or-year}
High

25637-99-4
Chinese Rare
Minnow
(Gobiocypris
rants)
Fresh
28-day
NOAEL = 0.5 mg
AI/L
0, 0.001,0.01,0.1,
0.5 mg/L
Renewal, Nominal,
Solvent: DMSO
Thiobarbituric
acid reactive
substances
{Zhang, 2008,
1927768@@auth
or-year}
High

25637-99-4
Chinese Rare
Minnow
(Gobiocypris
rants)
Fresh
42-day
LOAEL = 0.001 mg
AI/L
0, 0.001,0.01,0.1,
0.5 mg/L
Renewal, Nominal,
Solvent: DMSO
Glutathione
disulfide
(oxidized
glutathione)
{Zhang, 2008,
1927768@@auth
or-year}
High

25637-99-4
Chinese Rare
Minnow
(Gobiocypris
rants)
Fresh
42-day
34 mg/kg
O.OOlmg/L
Renewal, Nominal,
Solvent: DMSO
Residue; whole
body HBCD
concentration,
wet weight
{Zhang, 2008,
1927768@@auth
or-year}
High

25637-99-4
Chinese Rare
Minnow
(Gobiocypris
rants)
Fresh
42-day
654 mg/kg
0.5 mg/L
Renewal, Nominal,
Solvent: DMSO
Residue: whole
body HBCD
concentration,
wet weight
{Zhang, 2008,
1927768@@auth
or-year}
High

29

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
7-day
NOAEL = 0.000011
mg AI/L
0, 0.000011 mg/L
Flow-through, Measured,
Solvent: Methanol
Triiodothyronine;
Thyroxine;
Sodium
potassium
ATPase
{Lower, 2007,
1927956@@auth
or-year}
High
1927956
25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
14-day
NOAEL = 0.000011
mg AI/L
0, 0.000011 mg/L
Flow-through, Measured,
Solvent: Methanol
Triiodothyronine;
Thyroxine;
Sodium
potassium
ATPase
{Lower, 2007,
1927956@@auth
or-year}
High

25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
21-day
NOAEL = 0.000011
mg AI/L
0, 0.000011 mg/L
Flow-through, Measured,
Solvent: Methanol
Triiodothyronine;
Thyroxine;
Sodium
potassium
ATPase
{Lower, 2007,
1927956@@auth
or-year}
High

25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
28-day
NOAEL = 0.000011
mg AI/L
0, 0.000011 mg/L
Flow-through, Measured,
Solvent: Methanol
Triiodothyronine;
Sodium
potassium
ATPase
{Lower, 2007,
1927956@@auth
or-year}
High

25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
28-day
LOAEL = 0.000011
mg AI/L
0, 0.000011 mg/L
Flow-through, Measured,
Solvent: Methanol
Thyroxine;
{Lower, 2007,
1927956@@auth
or-year}
High

25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
7-day
LOAEL = 0.000011
mg AI/L
0, 0.000011 mg/L
Flow-through, Measured,
Solvent: Methanol
Smell/Sniff
{Lower, 2007,
1927956@@auth
or-year}
High

25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
17-day
LOAEL = 0.000011
mg AI/L
0, 0.000011 mg/L
Flow-through, Measured,
Solvent: Methanol
Smell/Sniff
{Lower, 2007,
1927956@@auth
or-year}
High

25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
29-day
LOAEL = 0.000011
mg AI/L
0, 0.000011 mg/L
Flow-through, Measured,
Solvent: Methanol
Smell/Sniff
{Lower, 2007,
1927956@@auth
or-year}
High

25637-99-4
Atlantic
salmon (Salmo
salar)
Fresh
30-day
NOAEL = 0.000011
mg AI/L
0, 0.000011 mg/L
Flow-through, Measured,
Solvent: Methanol
Mortality;
Growth: Length;
Growth: Weight;
Condition index
{Lower, 2007,
1927956@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
5-day
LOAEL = <500 mg
Al/kg bdwt
0, < 500 mg/kg bdwt
Intraperitoneal, Nominal
Cytochrome P1A
{Ronisz, 2004,
192782 l@@auth
or-year}
High
1927821
30

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CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
5-day
NOAEL = <500 mg
Al/kg bdwt
0 50, < 500 mg/kg
bdwt
Intraperitoneal, Nominal
DNA Adducts; 7-
Ethoxyresorufm
O-deethylase;
Glutathione S-
transferase
{Ronisz, 2004,
192782 l@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
5-day
NOAEL = <500 mg
Al/kg bdwt; Exp. 1
0, 50, < 500 mg/kg
bdwt
Intraperitoneal, Nominal
Liver somatic
index;
Glutathione
reductase
{Ronisz, 2004,
192782 l@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
5-day
NOAEL = <500 mg
Al/kg bdwt; Exp. 2
0, < 500 mg/kg bdwt
Intraperitoneal, Nominal
Liver somatic
index;
Glutathione
reductase;
Catalase
{Ronisz, 2004,
192782 l@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
28-day
NOAEL = 50 mg
Al/kg bdwt; LOAEL
= <500 mg Al/kg
bdwt
0, 50, < 500 mg/kg
bdwt
Intraperitoneal, Nominal
Liver somatic
index; 7-Ethoxy-
resorufin O-
deethylase
{Ronisz, 2004,
192782 l@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
28-day
NOAEL = <500 mg
Al/kg bdwt
0, 50, < 500 mg/kg
bdwt
Intraperitoneal, Nominal
Glutathione
reductase;
Catalase;
Glutathione S-
transferase
{Ronisz, 2004,
192782 l@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
5-day
LOAEL = 50 mg/kg
bdwt; Exp. 1
0, 50, < 500 mg/kg
bdwt
Intraperitoneal, Nominal
Catalase
{Ronisz, 2004,
192782 l@@auth
or-year}
High

25637-99-4
Indian Medaka
(Oryzias
melastigma)
Salt
5 days post
fertilization
LOAEL = 0.005 mg
AI/L
0, 0.005,0.02, 0.05
mg/L
Renewal, Nominal,
Solvent: DMSO
Heart rate
{Hong, 2014,
2343684@@auth
or-year}
High
2343684
25637-99-4
Indian Medaka
Salt
8 days post
LOAEL = 0.005 mg
0, 0.005,0.02, 0.05
Renewal, Nominal,
Heart rate;
{Hong, 2014,
High


(Oryzias
melastigma)

fertilization
AI/L
mg/L
Solvent: DMSO
Uncharacterized
arginine/ serine-
rich coiled-coil 1
mRNA
2343684@@auth
or-year}


25637-99-4
Indian Medaka
Salt
5 days post
NOAEL = 0.02 mg
0, 0.005, 0.02, 0.05
Renewal, Nominal,
Growth:
{Hong, 2014,
High


(iOryzias
melastigma)

fertilization
AI/L;
LOAEL = 0.05 mg
AI/L
mg/L
Solvent: DMSO
Abnormal; SV-
BA distance
2343684@@auth
or-year}


31

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Indian Medaka
(Oryzias
melastigma)
Salt
8 days post
fertilization
NOAEL = 0.005 mg
AI/L;
LOAEL = 0.02 mg
AI/L
0, 0.005, 0.02, 0.05
mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal; SV-
BA distance;
Coiled coil
domain
containing 106
protein mRNA;
Uncharacterized
transmembrane
and coiled-coil
domain family 3
mRNA
{Hong, 2014,
2343684@@auth
or-year}
High

25637-99-4
Indian Medaka
(iOryzias
melastigma)
Salt
5 days post
fertilization
NOAEL = 0.05 mg
AI/L;
LOAEL = 0.2 mg
AI/L
0, 0.02,0.05,0.2
mg/L
Renewal, Nominal,
Solvent: DMSO
Caspase 3;
Interleukin-1 beta
{Hong, 2014,
2343684@@auth
or-year}
High
25637-99-4
Indian Medaka
(Oryzias
melastigma)
Salt
8 days post
fertilization
NOAEL = 0.05 mg
AI/L;
LOAEL = 0.2 mg
AI/L
0, 0.02,0.05,0.2
mg/L
Renewal, Nominal,
Solvent: DMSO
Caspase 3;
Caspase 8;
Caspase 9; p53
mRNA;
Interleukin-1 beta
{Hong, 2014,
2343684@@auth
or-year}
High
25637-99-4
Indian Medaka
(iOryzias
melastigma)
Salt
5 days post
fertilization
NOAEL = 0.2 mg
AI/L
0, 0.02,0.05,0.2
mg/L
Renewal, Nominal,
Solvent: DMSO
Caspase 8;
Caspase 9
{Hong, 2014,
2343684@@auth
or-year}
High
25637-99-4
Indian Medaka
(iOryzias
melastigma)
Salt
5 days post
fertilization
NOAEL = 0.02 mg
AI/L; LOAEL = 0.05
mg AI/L
0, 0.02,0.05,0.2
mg/L
Renewal, Nominal,
Solvent: DMSO
p53 mRNA;
Tumor necrosis
factor-alpha
{Hong, 2014,
2343684@@auth
or-year}
High
25637-99-4
Indian Medaka
(iOryzias
melastigma)
Salt
First fry
NOAEL = 0.05 mg
AI/L;
LOAEL = 0.2 mg
AI/L
0, 0.02,0.05,0.2
mg/L
Renewal, Nominal,
Solvent: DMSO
p53 mRNA;
Interleukin-1
beta; Tumor
necrosis factor-
alpha
{Hong, 2014,
2343684@@auth
or-year}
High
25637-99-4
Indian Medaka
(iOryzias
melastigma)
Salt
8 days post
fertilization
NOAEL = 0.2 mg
AI/L
0, 0.02,0.05,0.2
mg/L
Renewal, Nominal,
Solvent: DMSO
Tumor necrosis
factor-alpha
{Hong, 2014,
2343684@@auth
or-year}
High
25637-99-4
Indian Medaka
(iOryzias
melastigma)
Salt
6 days post
fertilization
NOAEL = 0.005 mg
AI/L;
LOAEL = 0.02 mg
AI/L
0, 0.005,0.02, 0.05
mg/L
Renewal, Nominal,
Solvent: DMSO
8-Oxo-2'-deoxy-
guanosine
{Hong, 2014,
2343684@@auth
or-year}
High
32

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Indian Medaka
(Oryzias
melastigma)
Salt
8 days post
fertilization
LOAEL = 0.05 mg
AI/L
0, 0.005,0.02, 0.05
mg/L
Renewal, Nominal,
Solvent: DMSO
Uncharacterized
polycomb protein
mRNA;
Uncharacterized
short-chain de-
hydrogenase/redu
ctase family
mRNA; 40S
ribosomal protein
SAmRNA;
Brain-type fatty
acid binding
protein mRNA
{Hong, 2014,
2343684@@auth
or-year}
High

25637-99-4
Indian Medaka
(Oryzias
melastigma)
Salt
8 days post
fertilization
NOAEL = 0.02 mg
AI/L;
LOAEL = 0.05 mg
AI/L
0, 0.005,0.02, 0.05
mg/L
Renewal, Nominal,
Solvent: DMSO
Ribo se-pho sphate
pyrophosphate
kinase mRNA;
Uncharacterized
bromodomain and
WD repeat
domain
containing 3
mRNA;
Uncharacterized
parvalbumin beta-
like mRNA;
Ubiquitin-
conjugating
protein (E2)-like
mRNA
{Hong, 2014,
2343684@@auth
or-year}
High
25637-99-4
Indian Medaka
(iOryzias
melastigma)
Salt
8 days post
fertilization
NOAEL = 0.005 mg
AI/L;
LOAEL = 0.02 mg
AI/L
0, 0.005,0.02, 0.05
mg/L
Renewal, Nominal,
Solvent: DMSO
Uncharacterized
myosin regulatory
light chain
mRNA
{Hong, 2014,
2343684@@auth
or-year}
High
25637-99-4
Indian Medaka
(Oryzias
melastigma)
Salt
8 days post
fertilization
LOAEL = 0.005 mg
AI/L
0, 0.005,0.02, 0.05
mg/L
Renewal, Nominal,
Solvent: DMSO
Choriogenin L
mRNA
{Hong, 2014,
2343684@@auth
or-year}
High
25637-99-4
Indian Medaka
(Oryzias
melastigma)
Salt
8 days post
fertilization
LOAEL = 0.02 mg
AI/L
0, 0.005,0.02, 0.05
mg/L
Renewal, Nominal,
Solvent: DMSO
Vitellogenin 2
mRNA
{Hong, 2014,
2343684@@auth
or-year}
High
25637-99-4
Indian Medaka
(iOryzias
melastigma)
Salt
8 days post
fertilization
NOAEL = 0.05 mg
AI/L
0, 0.005,0.02, 0.05
mg/L
Renewal, Nominal,
Solvent: DMSO
Vitellogenin-like
protein
{Hong, 2014,
2343684@@auth
or-year}
High
33

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Indian Medaka
(Oryzias
melastigma)
Salt
17 days
post
fertilization
NOAEL = 0.02 mg
AI/L;
LOAEL = 0.05 mg
AI/L
0, 0.005,0.02, 0.05
mg/L
Renewal, Nominal,
Solvent: DMSO
Growth:
Abnormal;
malformations
{Hong, 2014,
2343684@@auth
or-year}
High

25637-99-4
Indian Medaka
(Oryzias
melastigma)
Salt
17 days
post
fertilization
NOAEL = 0.05 mg
AI/L
0, 0.005,0.02, 0.05
mg/L
Renewal, Nominal,
Solvent: DMSO
Mortality;
Hatching success;
Hatchout time
{Hong, 2014,
2343684@@auth
or-year}
High

134237-52-8
Indian Medaka
(Oryzias
melastigma)
Salt
5-day post
fertilization
NOAEL = 0.0084-
0.0163 mg AI/L;
LOAEL = 0.0165-
0.0324 mg AI/L
0, 0.0084-0.0163,
0.0165-0.0324,
0.1212-0.1568 mg/L
Renewal, Measured,
Solvent: DMSO
Heart rate
{Hong, 2015,
3350507@@auth
or-year}
High

134237-52-8
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
LOAEL = 0.0084-
0.0163 mg AI/L
0, 0.0084-0.0163,
0.0165-0.0324,
0.1212-0.1568 mg/L
Renewal, Measured,
Solvent: DMSO
Heart rate
{Hong, 2015,
3350507@@auth
or-year}
High
3350507
134237-52-8
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
NOAEL = 0.0165-
0.0324mg AI/L;
LOAEL = 0.1212-
0.1568 mg AI/L
0, 0.0084-0.0163,
0.0165-0.0324,
0.1212-0.1568 mg/L
Renewal, Measured,
Solvent: DMSO
Growth,
abnormal. SV-
BA distance;
Interleukin 1 beta
mRNA; Tumor
necrosis factor
mRNA
{Hong, 2015,
3350507@@auth
or-year}
High

134237-52-8
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
LOAEL = 0.0084-
0.0163 mg AI/L
0, 0.0084-0.0163,
0.1212-0.1568 mg/L
Renewal, Measured,
Solvent: DMSO
Caspase 3
{Hong, 2015,
3350507@@auth
or-year}
High

134237-52-8
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
NOAEL = 0.0084-
0.0163 mg AI/L;
LOAEL = 0.1212-
0.1568 mg AI/L
0, 0.0084-0.0163,
0.1212-0.1568 mg/L
Renewal, Measured,
Solvent: DMSO
Caspase 8;
Caspase 9
{Hong, 2015,
3350507@@auth
or-year}
High

134237-52-8
Indian Medaka
(Oryzias
melastigma)
Salt
8-day post
fertilization
NOAEL = 0.0084-
0.0163 mg AI/L;
LOAEL = 0.0165-
0.0324 mg AI/L
0, 0.0084-0.0163,
0.0165-0.0324,
0.1212-0.1568 mg/L
Renewal, Measured,
Solvent: DMSO
p53 mRNA
{Hong, 2015,
3350507@@auth
or-year}
High

134237-52-8
Indian Medaka
(iOryzias
melastigma)
Salt
>8-< 17-day
post
fertilization
NOAEL = 0.0017-
0.0324 mg AI/L;
LOAEL = 0.1212-
0.1568 mg AI/L
0, 0.0084-0.0163,
0.0165-0.0324,
0.1212-0.1568 mg/L
Renewal, Measured,
Solvent: DMSO
p53 mRNA,
newly hatched
larvae; Interleukin
1 beta mRNA,
newly hatched
larvae; Tumor
necrosis factor
mRNA; newly
hatched larvae
{Hong, 2015,
3350507@@auth
or-year}
High

34

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-52-8
Indian Medaka
(Oryzias
melastigma)
Salt
6-day post
fertilization
NOAEL = 0.0017-
0.0324 mg AI/L;
LOAEL = 0.1212-
0.1568 mg AI/L
0, 0.0084-0.0163,
0.0165-0.0324,
0.1212-0.1568 mg/L
Renewal, Measured,
Solvent: DMSO
8-Oxo-2'-
deoxyguanosine
{Hong, 2015,
3350507@@auth
or-year}
High

134237-52-8
Indian Medaka
(Oryzias
melastigma)
Salt
17-day post
fertilization
NOAEL = 0.0017-
0.0324 mg AI/L;
LOAEL =0.1212-
0.1568 mg AI/L
0, 0.0084-0.0163,
0.0165-0.0324,
0.1212-0.1568 mg/L
Renewal, Measured,
Solvent: DMSO
Growth:
Abnormal;
Malformation rate
{Hong, 2015,
3350507@@auth
or-year}
High
134237-52-8
Indian Medaka
(Oryzias
melastigma)
Salt
17-day post
fertilization
NOAEL =0.1212-
0.1568 mg AI/L
0, 0.0084-0.0163,
0.0165-0.0324,
0.1212-0.1568 mg/L
Renewal, Measured,
Solvent: DMSO
Mortality;
Hatching rate
{Hong, 2015,
3350507@@auth
or-year}
High
134237-51-7
Indian Medaka
(iOryzias
melastigma)
Salt
5-day post
fertilization
LOAEL = 0.1326-
0.1845 mg AI/L
0, 0.0082-0.0145,
0.0205-0.0341,
0.1326-0.1845 mg/L
Renewal, Measured,
Solvent: DMSO
Heart rate
{Hong, 2015,
3350507@@auth
or-year}
High
134237-51-7
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
LOAEL = 0.0082-
0.0145 mg AI/L
0, 0.0082-0.0145,
0.0205-0.0341,
0.1326-0.1845 mg/L
Renewal, Measured,
Solvent: DMSO
Heart rate
{Hong, 2015,
3350507@@auth
or-year}
High
134237-51-7
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
NOAEL = 0.021-
0.0341 mg AI/L;
LOAEL = 0.1326-
0.1845 mg AI/L
0, 0.0082-0.0145,
0.0205-0.0341,
0.1326-0.1845 mg/L
Renewal, Measured,
Solvent: DMSO
Growth,
abnormal. SV-
BA distance; p53
mRNA; Tumor
necrosis factor
mRNA
{Hong, 2015,
3350507@@auth
or-year}
High
134237-51-7
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
LOAEL = 0.0082-
0.0145 mg AI/L
0, 0.0082-0.0145,
0.1326-0.1845 mg/L
Renewal, Measured,
Solvent: DMSO
Caspase 3;
Caspase 9
{Hong, 2015,
3350507@@auth
or-year}
High
134237-51-7
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
NOAEL = 0.0082-
0.0145 mg AI/L;
LOAEL = 0.1326-
0.1845 mg AI/L
0, 0.0082-0.0145,
0.1326-0.1845 mg/L
Renewal, Measured,
Solvent: DMSO
Caspase 8
{Hong, 2015,
3350507@@auth
or-year}
High
134237-51-7
Indian Medaka
(Oryzias
melastigma)
Salt
>8-< 17-day
post
fertilization
NOAEL = 0.021-
0.0341 mg AI/L;
LOAEL = 0.1326-
0.1845 mg AI/L
0, 0.0082-0.0145,
0.0205-0.0341,
0.1326-0.1845 mg/L
Renewal, Measured,
Solvent: DMSO
p53 mRNA,
newly hatched
larvae; Tumor
necrosis factor
mRNA, newly
hatched larvae
{Hong, 2015,
3350507@@auth
or-year}
High
134237-51-7
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
NOAEL = 0.0082-
0.0145 mg AI/L;
LOAEL = 0.0205-
0.0341 mg AI/L
0, 0.0082-0.0145,
0.0205-0.0341,
0.1326-0.1845 mg/L
Renewal, Measured,
Solvent: DMSO
Interleukin 1 beta
mRNA
{Hong, 2015,
3350507@@auth
or-year}
High
35

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-51-7
Indian Medaka
(Oryzias
melastigma)
Salt
>8-< 17-day
post
fertilization
NOAEL = 0.1326-
0.1845 mgAI/L
0, 0.0082-0.0145,
0.0205-0.0341,
0.1326-0.1845 mg/L
Renewal, Measured,
Solvent: DMSO
Interleukin 1 beta
mRNA, newly
hatched larvae
{Hong, 2015,
3350507@@auth
or-year}
High

134237-51-7
Indian Medaka
(iOryzias
melastigma)
Salt
6-day post
fertilization
NOAEL = 0.021-
0.0341 mg AI/L;
LOAEL = 0.1326-
0.1845 mgAI/L
0, 0.0082-0.0145,
0.0205-0.0341,
0.1326-0.1845 mg/L
Renewal, Measured,
Solvent: DMSO
8-Oxo-2'-deoxy-
guanosine
{Hong, 2015,
3350507@@auth
or-year}
High
134237-51-7
Indian Medaka
(Oryzias
melastigma)
Salt
17-day post
fertilization
NOAEL = 0.1326-
0.1845 mgAI/L
0, 0.0082-0.0145,
0.0205-0.0341,
0.1326-0.1845 mg/L
Renewal, Measured,
Solvent: DMSO
Growth:
Abnormal,
Malformation
rate; Mortality;
Hatching rate
{Hong, 2015,
3350507@@auth
or-year}
High
134237-50-6
Indian Medaka
(iOryzias
melastigma)
Salt
5-day post
fertilization
LOAEL = 0.0097-
0.0141 mgAI/L
0,0.0097-0.0141,
0.0237-0.0375,
0.1252-0.1684 mg/L
Renewal, Measured,
Solvent: DMSO
Heart rate
{Hong, 2015,
3350507@@auth
or-year}
High
134237-50-6
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
LOAEL = 0.0097-
0.0141 mgAI/L
0,0.0097-0.0141,
0.0237-0.0375,
0.1252-0.1684 mg/L
Renewal, Measured,
Solvent: DMSO
Heart rate
{Hong, 2015,
3350507@@auth
or-year}
High
134237-50-6
Indian Medaka
(Oryzias
melastigma)
Salt
8-day post
fertilization
NOAEL = 0.024-
0.0375 mg AI/L
LOAEL = 0.1252-
0.1684 mgAI/L
0,0.0097-0.0141,
0.0237-0.0375,
0.1252-0.1684 mg/L
Renewal, Measured,
Solvent: DMSO
Growth,
Abnormal; SV-
BA length;
Tumor necrosis
factor mRNA
{Hong, 2015,
3350507@@auth
or-year}
High
134237-50-6
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
LOAEL = 0.0097-
0.0141 mgAI/L
0,0.0097-0.0141,
0.1252-0.1684 mg/L
Renewal, Measured,
Solvent: DMSO
Caspase 3
{Hong, 2015,
3350507@@auth
or-year}
High
134237-50-6
Indian Medaka
(iOryzias
melastigma)
Salt
8-day post
fertilization
NOAEL = 0.0097-
0.0141 mgAI/1;
LOAEL = 0.1252-
0.1684 mgAI/L
0,0.0097-0.0141,
0.1252-0.1684 mg/L
Renewal, Measured,
Solvent: DMSO
Caspase 8;
Caspase 9
{Hong, 2015,
3350507@@auth
or-year}
High
134237-50-6
Indian Medaka
(Oryzias
melastigma)
Salt
8-day post
fertilization
NOAEL = 0.0097-
0.0141 mgAI/L;
LOAEL = 0.0237-
0.0375 mg/AI/L
0,0.0097-0.0141,
0.0237-0.0375,
0.1252-0.1684 mg/L
Renewal, Measured,
Solvent: DMSO
p53 mRNA;
Interleukin 1 beta
mRNA
{Hong, 2015,
3350507@@auth
or-year}
High
36

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CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-50-6
Indian Medaka
(Oryzias
melastigma)
Salt
>8-< 17-day
post
fertilization
NOAEL = 0.024-
0.0375 mg AI/L;
LOAEL = 0.1252-
0.1684 mg AI/L
0,0.0097-0.0141,
0.0237-0.0375,
0.1252-0.1684 mg/L
Renewal, Measured,
Solvent: DMSO
p53 mRNA,
newly hatched
larvae; Interleukin
1 beta mRNA,
newly hatched
larvae; Tumor
necrosis factor
mRNA, newly
hatched larvae
{Hong, 2015,
3350507@@auth
or-year}
High

134237-50-6
Indian Medaka
(Oryzias
melastigma)
Salt
6-day post
fertilization
NOAEL = 0.024-
0.0375 mg AI/L;
LOAEL = 0.1252-
0.1684 mg AI/L
0,0.0097-0.0141,
0.0237-0.0375,
0.1252-0.1684 mg/L
Renewal, Measured,
Solvent: DMSO
8-Oxo-2'-
deoxyguanosine
{Hong, 2015,
3350507@@auth
or-year}
High
134237-50-6
Indian Medaka
(Oryzias
melastigma)
Salt
17-day post
fertilization
NOAEL = 0.024-
0.0375 mg AI/L;
LOAEL = 0.1252-
0.1684 mg AI/L
0,0.0097-0.0141,
0.0237-0.0375,
0.1252-0.1684 mg/L
Renewal, Measured,
Solvent: DMSO
Growth:
Abnormal.
Malformation rate
{Hong, 2015,
3350507@@auth
or-year}
High
134237-50-6
Indian Medaka
(iOryzias
melastigma)
Salt
17-day post
fertilization
NOAEL = 0.1252-
0.1684 mg AI/L
0,0.0097-0.0141,
0.0237-0.0375,
0.1252-0.1684 mg/L
Renewal, Measured,
Solvent: DMSO
Mortality; Hatch
rate
{Hong, 2015,
3350507@@auth
or-year}
High
134237-52-8
Zebrafish
(.Danio rerio)
Fresh
7-day
NOAEL = 0.01 mg
AI/L;
LOAEL = 0.1 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
Cyplbl mRNA;
7-Ethoxyresorufin
O-deethylase;
Cytochrome P1A
messenger RNA;
Aryl hydrocarbon
receptor lb
mRNA
{Du, 2015,
3350537@@auth
or-year}
High

134237-52-8
Zebrafish
(Danio rerio)
Fresh
21-day
LOAEL = 0.001 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
Cyplbl mRNA
{Du, 2015,
3350537@@auth
or-year}
High
3350537
134237-52-8
Zebrafish
{Danio rerio)
Fresh
7-day
NOAEL = 0.001 mg
AI/L;
LOAEL = 0.01 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
Cytochrome
P450, family 1,
subfamily C,
polypeptide 1
mRNA; Aryl
hydrocarbon
receptor la
mRNA; Aryl
hydrocarbon
receptor 2 mRNA
{Du, 2015,
3350537@@auth
or-year}
High
37

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-52-8
Zebrafish
(Danio rerio)
Fresh
21-day
NOAEL = 0.001 mg
AI/L;
LOAEL = 0.01 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
Cytochrome
P450, family 1,
subfamily C,
polypeptide 1
mRNA; 7-
Ethoxyresorufm
O-deethylase;
Cytochrome P1A
messenger RNA;
Aryl hydrocarbon
receptor la
mRNA; Aryl
hydrocarbon
receptor lb
mRNA; Aryl
hydrocarbon
receptor 2 mRNA
{Du, 2015,
3350537@@auth
or-year}
High

134237-52-8
Zebrafish
{Danio rerio)
Fresh
21-day
NOAEL = 0.1 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
Liver somatic
index; Mortality
{Du, 2015,
3350537@@auth
or-year}
High
134237-51-7
Zebrafish
(Danio rerio)
Fresh
7-day
NOAEL = 0.001 mg
AI/L;
LOAEL = 0.01 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
Cyplbl mRNA;
Cytochrome
P450, family 1,
subfamily C,
polypeptide 1
mRNA; Aryl
hydrocarbon
receptor la
mRNA; Aryl
hydrocarbon
receptor 2 mRNA
{Du, 2015,
3350537@@auth
or-year}
High
134237-51-7
Zebrafish
{Danio rerio)
Fresh
21-day
NOAEL = 0.001 mg
AI/L;
LOAEL = 0.01 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
Cyplbl mRNA;
Cytochrome
P450, family 1,
subfamily C,
polypeptide 1
mRNA;
Cytochrome P1A
messenger RNA;
Aryl hydrocarbon
receptor lb
mRNA
{Du, 2015,
3350537@@auth
or-year}
High
38

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-51-7
Zebrafish
(Danio rerio)
Fresh
7-day
NOAEL = 0.01 mg
AI/L;
LOAEL = 0.1 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase;
Cytochrome P1A
messenger RNA;
Aryl hydrocarbon
receptor lb
mRNA
{Du, 2015,
3350537@@auth
or-year}
High

134237-51-7
Zebrafish
{Danio rerio)
Fresh
21-day
NOAEL = 0.01 mg
AI/L;
LOAEL = 0.1 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase;
Aryl hydrocarbon
receptor 2 mRNA
{Du, 2015,
3350537@@auth
or-year}
High

134237-51-7
Zebrafish
(Danio rerio)
Fresh
21-day
NOAEL = 0.1 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
Aryl hydrocarbon
receptor la
mRNA; Liver
somatic index;
Mortality
{Du, 2015,
3350537@@auth
or-year}
High

134237-50-6
Zebrafish
Fresh
7-day
NOAEL = 0.001 mg
0,0.001,0.01,0.1
Renewal, Nominal,
Cyplbl mRNA;
{Du, 2015,
High


{Danio rerio)


AI/L;
LOAEL = 0.01 mg
AI/L
mg/L
Solvent: DMSO
Cytochrome
P450, family 1,
subfamily C,
polypeptide 1
mRNA; Aryl
hydrocarbon
receptor la
mRNA; Aryl
hydrocarbon
receptor lb
mRNA
3350537@@auth
or-year}


134237-50-6
Zebrafish
Fresh
21-day
NOAEL = 0.001 mg
0,0.001,0.01,0.1
Renewal, Nominal,
Cyplbl mRNA;
{Du, 2015,
High


{Danio rerio)


AI/L;
LOAEL = 0.01 mg
AI/L
mg/L
Solvent: DMSO
Cytochrome
P450, family 1,
subfamily C,
polypeptide 1
mRNA; Aryl
hydrocarbon
receptor la
mRNA; Aryl
hydrocarbon
receptor lb
mRNA; Aryl
hydrocarbon
receptor 2 mRNA
3350537@@auth
or-year}


39

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-50-6
Zebrafish
(Danio rerio)
Fresh
7-day
NOAEL = 0.1 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase;
Aryl hydrocarbon
receptor 2 mRNA
{Du, 2015,
3350537@@auth
or-year}
High

134237-50-6
Zebrafish
(Danio rerio)
Fresh
21-day
NOAEL = 0.01 mg
AI/L;
LOAEL = 0.1 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
7-Ethoxyresorufin
O-deethylase;
Cytochrome P1A
messenger RNA
{Du, 2015,
3350537@@auth
or-year}
High

134237-50-6
Zebrafish
{Danio rerio)
Fresh
7-day
NOAEL = 0.01 mg
AI/L;
LOAEL = 0.1 mg
AI/L
0,0.001,0.01,0.1
mg/L
Renewal, Nominal,
Solvent: DMSO
Cytochrome P1A
messenger RNA
{Du, 2015,
3350537@@auth
or-year}
High

134237-50-6
Zebrafish
Fresh
21-day
NOAEL = 0.1 mg
0,0.001,0.01,0.1
Renewal, Nominal,
Liver somatic
{Du, 2015,
High


{Danio rerio)


AI/L
mg/L
Solvent: DMSO
index; Mortality
3350537@@auth
or-year}


134237-52-8
Zebrafish
{Danio rerio)
Fresh
7-day
NOAEL = 0.02284
mg Al/kg
0.00084, 0.02284
mg/kg
Food, Measured; lipid-
corrected
Triiodothyronine;
Thyroxine;
Thyroid gland
epithelial cell
height; Liver
somatic index
{Palace, 2010,
1403364@@auth
or-year}
High
1403364
134237-52-8
Zebrafish
{Danio rerio)
Fresh
14-day
NOAEL = 0.02284
mg Al/kg
0.00084, 0.02284
mg/kg
Food, Measured; lipid-
corrected
Triiodothyronine;
Thyroxine; 7-
Ethoxyresorufm
O-deethylase; T4
outer ring
deiodinase
enzyme activity;
Thyroid gland
epithelial cell
height; Liver
somatic index
{Palace, 2010,
1403364@@auth
or-year}
High

134237-52-8
Zebrafish
{Danio rerio)
Fresh
56-day
LOAEL = 0.02284
mg Al/kg
0.00084, 0.02284
mg/kg
Food, Measured; lipid-
corrected
Triiodothyronine;
Thyroxine; T4
outer ring
deiodinase
enzyme activity;
Thyroid gland
epithelial cell
height
{Palace, 2010,
1403364@@auth
or-year}
High

40

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-52-8
Zebrafish
(Danio rerio)
Fresh
7-day
LOAEL = 0.02284
mg Al/kg
0.00084, 0.02284
mg/kg
Food, Measured; lipid-
corrected
7-Ethoxyresorufin
O-deethylase
{Palace, 2010,
1403364@@auth
or-year}
High

134237-52-8
Zebrafish
{Danio rerio)
Fresh
56-day
NOAEL = 0.02284
mg Al/kg
0.00084, 0.02284
mg/kg
Food, Measured; lipid-
corrected
7-Ethoxyresorufin
O-deethylase;
Liver somatic
index; Uridine
diphosphate
glucuronyl
transferase, UDP
glucuronyl
transferase
{Palace, 2010,
1403364@@auth
or-year}
High

134237-52-8
Zebrafish
Fresh
168-
NOAEL = 0.02284
0.00084, 0.02284
Food, Measured; lipid-
Growth: Weight
{Palace, 2010,
High


{Danio rerio)

day *(112-
day
depuration
period after
56-day
exposure)
mg Al/kg
mg/kg
corrected

1403364@@auth
or-year}


134237-51-7
Zebrafish
{Danio rerio)
Fresh
7-day
NOAEL = 0.01184
mg Al/kg
0,0.01184 mg/kg
Food, Measured; lipid-
corrected
Triiodothyronine;
Thyroxine;
Thyroid gland
epithelial cell
height; Liver
somatic index
{Palace, 2010,
1403364@@auth
or-year}
High

134237-51-7
Zebrafish
{Danio rerio)
Fresh
14-day
NOAEL = 0.01184
mg Al/kg
0,0.01184 mg/kg
Food, Measured; lipid-
corrected
Triiodothyronine;
Thyroxine; 7-
Ethoxyresorufm
O-deethylase; T4
outer ring
deiodinase
enzyme activity;
Thyroid gland
epithelial cell
height; Liver
somatic index
{Palace, 2010,
1403364@@auth
or-year}
High

134237-51-7
Zebrafish
{Danio rerio)
Fresh
56-day
NOAEL = 0.01184
mg Al/kg
0,0.01184 mg/kg
Food, Measured; lipid-
corrected
Triiodothyronine;
Thyroid gland
epithelial cell
height; Liver
somatic index
{Palace, 2010,
1403364@@auth
or-year}
High

41

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-51-7
Zebrafish
(Danio rerio)
Fresh
56-day
LOAEL = 0.01184
mg Al/kg
0, 0.01184 mg/kg
Food, Measured; lipid-
corrected
Thyroxine; 7-
Ethoxyresorufm
O-deelliylase; T4
outer ring
deiodinase
enzyme activity;
Uridine
diphosphate
glucuronyl
transferase, UDP
glucuronyl
transferase
{Palace, 2010,
1403364@@auth
or-year}
High

134237-51-7
Zebrafish
{Danio rerio)
Fresh
7-day
LOAEL = 0.01184
mg Al/kg
0, 0.01184 mg/kg
Food, Measured; lipid-
corrected
7-Ethoxyresorufin
O-deethylase
{Palace, 2010,
1403364@@auth
or-year}
High

134237-51-7
Zebrafish
{Danio rerio)
Fresh
168-day*
(112-day
depuration
period after
56-day
exposure)
NOAEL = 0.01184
mg Al/kg
0,0.01184 mg/kg
Food, Measured; lipid-
corrected
Growth: Weight
rate
{Palace, 2010,
1403364@@auth
or-year}
High

134237-50-6
Zebrafish
{Danio rerio)
Fresh
7-day
NOAEL =
0.02914 mg Al/kg
0.00047,0.02914
mg/kg
Food, Measured; lipid-
corrected
Triiodothyronine;
Thyroxine;
Thyroid gland
epithelial cell
height; Liver
somatic index
{Palace, 2010,
1403364@@auth
or-year}
High

134237-50-6
Zebrafish
{Danio rerio)
Fresh
14-day
NOAEL=
0.02914 mg Al/kg
0.00047,0.02914
mg/kg
Food, Measured; lipid-
corrected
Triiodothyronine;
Thyroxine; 7-
Ethoxyresorufm
O-deelliylase; T4
outer ring
deiodinase
enzyme activity;
Thyroid gland
epithelial cell
height; Liver
somatic index
{Palace, 2010,
1403364@@auth
or-year}
High

42

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-50-6
Zebrafish
(Danio rerio)
Fresh
56-day
NOAEL =
0.02914 mgAI/kg
0.00047,0.02914
mg/kg
Food, Measured; lipid-
corrected
Triiodothyronine;
Thyroid gland
epithelial cell
height; Liver
somatic index
{Palace, 2010,
1403364@@auth
or-year}
High

134237-50-6
Zebrafish
(Danio rerio)
Fresh
56-day
LOAEL =
0.02914 mgAI/kg
0.00047,0.02914
mg/kg
Food, Measured; lipid-
corrected
Thyroxine; 7-
Ethoxyresorufm
O-deelliylase; T4
outer ring
deiodinase
enzyme activity;
Uridine
diphosphate
glucuronyl
transferase, UDP
glucuronyl
transferase
{Palace, 2010,
1403364@@auth
or-year}
High
134237-50-6
Zebrafish
{Danio rerio)
Fresh
7-day
LOAEL =
0.02914 mgAI/kg
0.00047,0.02914
mg/kg
Food, Measured; lipid-
corrected
7-Ethoxyresorufin
O-deethylase
{Palace, 2010,
1403364@@auth
or-year}
High
134237-50-6
Zebrafish
{Danio rerio)
Fresh
168-day*
(112-day
depuration
period after
56-day
exposure)
NOAEL=
0.02914 mgAI/kg
0.00047,0.02914
mg/kg
Food, Measured; lipid-
corrected
Growth: Weight
{Palace, 2010,
1403364@@auth
or-year}
High
25637-99-4
Rainbow trout
{Oncorhynchus
mykiss)
Fresh
35-day
Steady-State BCF
(edible tissue) =
6,531
0.00018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High
1928244
25637-99-4
Rainbow trout
{Oncorhynchus
mykiss)
Fresh
35-day
Steady-State BCF
(edible tissue) =
4,650
0.0018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High
25637-99-4
Rainbow trout
{Oncorhynchus
mykiss)
Fresh
35-day
Steady-State BCF
(non-edible tissue) =
20,726
0.00018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High
25637-99-4
Rainbow trout
{Oncorhynchus
mykiss)
Fresh
35-day
Steady-State BCF
(non-edible tissue) =
12,866
0.0018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High
43

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
35-day
Steady-State BCF
(whole body) =
13,085
0.00018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
35-day
Steady-State BCF
(whole body) = 8,974
0.0018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
35-day
Kinetic BCF (edible
tissue) = 14,039
0.00018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
35-day
Kinetic BCF (edible
tissue) = 9,826
0.0018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
35-day
Kinetic BCF (non-
edible tissue) =
30,242
0.00018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High

25637-99-4
Rainbow trout
(iOncorhynchus
mykiss)
Fresh
35-day
Kinetic BCF (non-
edible tissue) =
23,303
0.0018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High

25637-99-4
Rainbow trout
(iOncorhynchus
mykiss)
Fresh
35-day
Kinetic BCF (whole
body) = 21,940
0.00018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High

25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
35-day
Kinetic BCF (whole
body) = 16,450
0.0018 mg/L
Flow-through, Measured,
Solvent: Acetone
Residue;
Bioconcentration
{Wildlife Intl
LTD, 2000,
1928244@@auth
or-year}
High

134237-52-8
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Mortality;
Weight; Length;
Condition
{Palace, 2008,
1409610@@auth
or-year}
High
1409610
44

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-52-8
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 34
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery: muscle
tissue, gallbladder
tissue, thyroid
tissue, intestine
tissue, viscera
tissue, liver
tissue, blood
{Palace, 2008,
1409610@@auth
or-year}
High

134237-52-8
Rainbow trout
(iOncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 36
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery:muscle
tissue, gallbladder
tissue, thyroid
tissue, intestine
tissue, viscera
tissue, liver
tissue, blood
{Palace, 2008,
1409610@@auth
or-year}
High

134237-52-8
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 38
NOAEL = 0.005
mg/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery: muscle
tissue, gallbladder
tissue, thyroid
tissue, intestine
tissue, viscera
tissue, liver
tissue, blood
{Palace, 2008,
1409610@@auth
or-year}
High

134237-52-8
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 46
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery: muscle
tissue; intestine
tissue, viscera
tissue, liver tissue
{Palace, 2008,
1409610@@auth
or-year}
High

134237-52-8
Rainbow trout
(iOncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 46
LOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery: gallblad
der tissue, thyroid
tissue, blood
{Palace, 2008,
1409610@@auth
or-year}
High

134237-52-8
Rainbow trout
(iOncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 34
LOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Type II
iodothyronine
deiodinase
{Palace, 2008,
1409610@@auth
or-year}
High

134237-51-7
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 32
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Mortality,
Weight, Length,
Condition
{Palace, 2008,
1409610@@auth
or-year}
High

45

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CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-51-7
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 34
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery: muscle
tissue, gallbladder
tissue, thyroid
tissue, intestine
tissue, viscera
tissue, liver
tissue, blood
{Palace, 2008,
1409610@@auth
or-year}
High

134237-51-7
Rainbow trout
(iOncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 36
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery: muscle
tissue, gallbladder
tissue, thyroid
tissue, intestine
tissue, viscera
tissue, liver
tissue, blood
{Palace, 2008,
1409610@@auth
or-year}
High

134237-51-7
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 38
LOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery: muscle
tissue,
{Palace, 2008,
1409610@@auth
or-year}
High

134237-51-7
Rainbow trout
(iOncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 46
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery: muscle
tissue, intestine
tissue, liver
tissue
{Palace, 2008,
1409610@@auth
or-year}
High

134237-51-7
Rainbow trout
(iOncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 38
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery,
gallbladder tissue,
thyroid tissue,
intestine tissue,
viscera tissue,
liver tissue, blood
{Palace, 2008,
1409610@@auth
or-year}
High

134237-51-7
Rainbow trout
(iOncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 46
LOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery,
gallbladder tissue,
thyroid tissue,
viscera tissue,
blood
{Palace, 2008,
1409610@@auth
or-year}
High

134237-51-7
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 34
LOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Type II
iodothyronine
deiodinase
{Palace, 2008,
1409610@@auth
or-year}
High

46

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-50-6
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 32
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Mortality,
Weight, Length,
Condition
{Palace, 2008,
1409610@@auth
or-year}
High

134237-50-6
Rainbow trout
(iOncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 34
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery: muscle
tissue, gallbladder
tissue, thyroid
tissue, intestine
tissue, viscera
tissue, liver
tissue, blood;
Type II
iodothyronine
deiodinase
{Palace, 2008,
1409610@@auth
or-year}
High

134237-50-6
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 36
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery:muscle
tissue, gallbladder
tissue, thyroid
tissue, intestine
tissue, viscera
tissue, liver
tissue, blood
{Palace, 2008,
1409610@@auth
or-year}
High

134237-50-6
Rainbow trout
(iOncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 38
LOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery: muscle
tissue
{Palace, 2008,
1409610@@auth
or-year}
High

134237-50-6
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 46
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery: muscle
tissue, gallbladder
tissue, thyroid
tissue, intestine
tissue, viscera
tissue, liver tissue
{Palace, 2008,
1409610@@auth
or-year}
High

134237-50-6
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 38
NOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery:
gallbladder tissue,
thyroid tissue,
intestine tissue,
viscera tissue,
liver tissue, blood
{Palace, 2008,
1409610@@auth
or-year}
High

134237-50-6
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
> 32-day,
measured
on day 46
LOAEL = 0.005 mg
Al/kg
0, 0.005 mg/kg
Food, Nominal; Solvent:
Corn oil
Thyroxine; %
recovery, blood
{Palace, 2008,
1409610@@auth
or-year}
High

47

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
25637-99-4
Common sole
(.Solea solea)
Salt
6-day
NOEC = 0.25 mg/L
0, 0,0.025,0.08, 0.25
mg/L
Renewal, Nominal;
Solvent: DMSO
Hatching success
{Foekema, 2014,
2343709@@auth
or-year}
High
2343709
25637-99-4
Common sole
{Solea solea)
Salt
6-day
exposure*
followed by
34-day obs.
In clean
water
Internal Effect
Concentration
(IEC)5o = >12,400
mg/kg lipid weight
2,280 - 12,400
mg/kg lipid weight
Renewal, Measured
Mortality;
Growth:
completion of
metamorphosis
{Foekema, 2014,
2343709@@auth
or-year}
High

134237-52-8
Zebrafish
(Danio rerio)
Fresh
42-day
NOAEL = 0.04482
mg Al/kg
0, 0.00486, 0.04482
mg/kg dry wt
Food, Measured
Growth: Weight;
% Lipid
{Du, 2012,
1927579@@auth
or-year}
High
1927579
134237-52-8
Zebrafish
{Danio rerio)
Fresh
42-day
NR-ZERO = 0.04482
mg Al/kg
0, 0.00486, 0.04482
mg/kg dry wt
Food, Measured
Mortality
{Du, 2012,
1927579@@auth
or-year}
High

134237-52-8
Zebrafish
{Danio rerio)
Fresh
42-day
BMF = 7.61
0.0048 mg/kg dry wt
Food, Measured
Residue:
biomagnification
{Du, 2012,
1927579@@auth
or-year}
High

134237-52-8
Zebrafish
{Danio rerio)
Fresh
42-day
BMF = 7.76
0.04482 mg/kg dry
wt
Food, Measured
Residue:
biomagnification
{Du, 2012,
1927579@@auth
or-year}
High

134237-51-7
Zebrafish
{Danio rerio)
Fresh
42-day
NOAEL = 0.04748
mg Al/kg
0, 0.00452, 0.04748
mg/kg dry wt
Food, Measured
Growth: Weight;
% Lipid
{Du, 2012,
1927579@@auth
or-year}
High

134237-51-7
Zebrafish
{Danio rerio)
Fresh
42-day
NR-ZERO = 0.04748
mg Al/kg
0, 0.00452, 0.04748
mg/kg dry wt
Food, Measured
Mortality
{Du, 2012,
1927579@@auth
or-year}
High

134237-51-7
Zebrafish
{Danio rerio)
Fresh
42-day
BMF = 11.63
0.00452 mg/kg dry
wt
Food, Measured
Residue:
biomagnification
{Du, 2012,
1927579@@auth
or-year}
High

134237-51-7
Zebrafish
{Danio rerio)
Fresh
42-day
BMF = 7.34
0.04748 mg/kg dry
wt
Food, Measured
Residue:
biomagnification
{Du, 2012,
1927579@@auth
or-year}
High

134237-50-6
Zebrafish
{Danio rerio)
Fresh
42-day
NOAEL = 0.04576
mg Al/kg
0, 0.00443, 0.04576
mg/kg dry wt
Food, Measured
Growth: Weight;
% Lipid
{Du, 2012,
1927579@@auth
or-year}
High

134237-50-6
Zebrafish
{Danio rerio)
Fresh
42-day
NR-ZERO = 0.04576
mg Al/kg
0, 0.00443, 0.04576
mg/kg dry wt
Food, Measured
Mortality
{Du, 2012,
1927579@@auth
or-year}
High

134237-50-6
Zebrafish
{Danio rerio)
Fresh
42-day
BMF = 29.71
0.00443 mg/kg dry
wt
Food, Measured
Residue:
biomagnification
{Du, 2012,
1927579@@auth
or-year}
High

48

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-50-6
Zebrafish
(Danio rerio)
Fresh
42-day
BMF = 12.33
0.04576 mg/kg dry
wt
Food, Measured
Residue:
biomagnification
{Du, 2012,
1927579@@auth
or-year}
High

25637-99-4
Threespine
Stickleback
(Gasterosteus
aculeatus)
Salt
30-day
NOAEL = 0.0003 mg
AI/L
0, 0.00003, 0.0003
mg/L
Flow-through, Nominal,
Solvent: Acetone
DNA methylation
{Aniagu, 2008,
1412194@@auth
or-year}
Low
1412194
25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
33-day
NOEC = 0.0037 mg
AI/L
0, 0, 0.00025,
0.00047, 0.00083,
0.0018, 0.0037 mg/L
Flow-through, Measured.
Solvent: Acetone
Hatching success
{Drottar, 2001,
4796184@@auth
or-year}
This study
needs to be
reviewed
4796184
25637-99-4
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
61-day
NOEC = 0.0037 mg
AI/L
0, 0, 0.00025,
0.00047, 0.00083,
0.0018, 0.0037 mg/L
Flow-through, Measured.
Solvent: Acetone
Mortality;
Growth: Weight;
Growth: Length;
Time to Swim-up
{Drottar, 2001,
4796184@@auth
or-year}


134237-52-8
Common Carp
(Cyprinus
carpio)
Fresh
60-day
Kinetic BCF (gill) =
237
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High
2343723
134237-52-8
Common Carp
(iCyprinus
carpio)
Fresh
60-day
Kinetic BCF
(viscera) =584
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-52-8
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Kinetic BCF
(muscle) = 221
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-52-8
Common Carp
(Cyprinus
carpio)
Fresh
60-day
Kinetic BCF (skin) =
227
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-52-8
Common Carp
(Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF (gill) =
950
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-52-8
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF
(viscera) = 1,730
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-52-8
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF
(muscle) = 1,220
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-52-8
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF (skin) =
1,610
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-51-7
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Kinetic BCF (gill) =
322
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

49

-------
PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-51-7
Common Carp
(Cyprinus
carpio)
Fresh
60-day
Kinetic BCF
(viscera) = 642
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-51-7
Common Carp
(iCyprinus
carpio)
Fresh
60-day
Kinetic BCF
(muscle) = 187
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-51-7
Common Carp
(iCyprinus
carpio)
Fresh
60-day
Kinetic BCF (skin) =
204
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-51-7
Common Carp
(Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF (gill) =
1,290
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-51-7
Common Carp
(Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF
(viscera) = 1,900
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-51-7
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF
(muscle) = 1,030
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-51-7
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF (skin) =
1,440
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-50-6
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Kinetic BCF (gill) =
8,580
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-50-6
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Kinetic BCF
(viscera) = 11,500
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-50-6
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Kinetic BCF
(muscle) = 5,570
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-50-6
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Kinetic BCF (skin) =
6,400
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-50-6
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF (gill) =
34,500
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-50-6
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF
(viscera) = 34,200
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

134237-50-6
Common Carp
{Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF
(muscle) = 30,700
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

50

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-50-6
Common Carp
(Cyprinus
carpio)
Fresh
60-day
Lipid-Normalized
Kinetic BCF (skin) =
45,200
0, 0.001 mg/L
Renewal, Measured
Residue;
Bioconcentration
{Zhang, 2014,
2343723@@auth
or-year}
High

25637-99-4
Starry,
European
Flounder
(Platichthys
flesus)
Brackish
Fresh
78-day
NOAEL = 3000
mg/kg lipid diet with
800 mg/kg TOC
sediment OR
0 mg/kg lipid diet
with 8000 mg/kg
TOC sediment
Diet (mg/kg
lipid)/sediment
(mg/kg total organic
carbon): 0/0;
0.3/0.08; 3/0.8; 30/8;
300/80; 3000/800;
0/8000
Multiple routes (diet and
sediment), Nominal,
Solvent: Acetone
Thyroxine;
Aromatase; 7-
Ethoxyresorufm
O-deethylase;
Triiodothyronine;
Benzylresorufm
O-deethylase;
Pentylresorufin
O-deethylase
{Kuiper, 2007,
1412802@@auth
or-year}
High
1412.302
25637-99-4
Starry,
European
Flounder
(.Platichthys
flesus)
Brackish
78-day
NOAEL = 30 mg/kg
lipid diet with 8
mg/kg TOC
sediment; LOAEL =
300 mg/kg lipid diet
with 80 mg/kg TOC
sediment
Diet (mg/kg
lipid)/sediment
(mg/kg total organic
carbon): 0/0;
0.3/0.08; 3/0.8; 30/8;
300/80; 3000/800;
0/8000
Multiple routes (diet and
sediment), Nominal,
Solvent: Acetone
Residue: a-
HBCD
concentration in
muscle; P-HBCD
concentration in
muscle
{Kuiper, 2007,
1412802@@auth
or-year}
High

25637-99-4
Starry,
European
Flounder
(.Platichthys
flesus)
Brackish
78-day
LOAEL = 0.3 mg/kg
lipid diet with 0.08
mg/kg TOC sediment
Diet (mg/kg
lipid)/sediment
(mg/kg total organic
carbon): 0/0;
0.3/0.08; 3/0.8; 30/8;
300/80; 3000/800;
0/8000
Multiple routes (diet and
sediment), Nominal,
Solvent: Acetone
Residue; y-HBCD
concentration in
muscle
{Kuiper, 2007,
1412802@@auth
or-year}
High

134237-52-8
Rainbow trout
(Oncorhynchus
mykiss)
Fresh
168-day
(56-day
exposure to
treated food
then 112
days
untreated
food)
BMF = 7.2
0.0003, 0.02284
mg/kg
Food, Measured; Lipid-
corrected
Residue;
biomagnification
{Law, 2006,
1443861@@auth
or-year}
Low
1443861
51

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Water
Type
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data Quality
Ratings
HERO ID.
134237-51-7
Rainbow trout
Fresh
168-day
BMF = 4.3
0.0003,0.01184
Food, Measured; Lipid-
Residue;
{Law, 2006,
Low


(Oncorhynchus
mykiss)

(56-day
exposure to
treated food
then 112
days
untreated
food)

mg/kg
corrected
biomagnification
1443861@@auth
or-year}


134237-50-6
Rainbow trout
Fresh
168-day
BMF = 9.2
0.0003,0.02914
Food, Measured; Lipid-
Residue;
{Law, 2006,
Low


(iOncorhynchus
mykiss)

(56-day
exposure to
treated food
then 112
days
untreated
food)

mg/kg
corrected
biomagnification
1443861@@auth
or-year}


Amphibians

25637-99-4
African clawed
frog (Xenopus
laevis)
Fresh
1-day
NOAEL = 6.417 mg
AI/L
0, 0.64, 6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Developmental:
Tail resorption
{Schriks, 2006,
938764@@autho
r-year}
Unacceptable

25637-99-4
African clawed
frog (Xenopus
laevis)
Fresh
2-day
NOAEL = 6.417mg
AI/L
0, 0.64, 6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Developmental:
Tail resorption
{Schriks, 2006,
938764@@autho
r-year}
Unacceptable
938764
25637-99-4
African clawed
frog (Xenopus
laevis)
Fresh
3-day
NOAEL = 6.417 mg
AI/L
0, 0.64, 6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Developmental:
Tail resorption
{Schriks, 2006,
938764@@autho
r-year}
Unacceptable

25637-99-4
African clawed
frog (Xenopus
laevis)
Fresh
4-day
NOAEL = 6.417 mg
AI/L
0, 0.64, 6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Developmental:
Tail resorption
{Schriks, 2006,
938764@@autho
r-year}
Unacceptable

25637-99-4
African clawed
frog (Xenopus
laevis)
Fresh
5-day
NOAEL = 6.417 mg
AI/L
0, 0.64, 6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Developmental:
Tail resorption
{Schriks, 2006,
938764@@autho
r-year}
Unacceptable

25637-99-4
African clawed
frog (Xenopus
laevis)
Fresh
6-day
NOAEL = 6.417 mg
AI/L
0, 0.64, 6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Developmental:
Tail resorption
{Schriks, 2006,
938764@@autho
r-year}
Unacceptable

25637-99-4
African clawed
frog (Xenopus
laevis)
Fresh
8-day
NOAEL = 0.642 mg
AI/L
0, 0.64 mg/L
Renewal, Nominal,
Solvent: DMSO
Cell proliferation
{Schriks, 2006,
938764@@autho
r-year}
Unacceptable

25637-99-4
African clawed
frog (Xenopus
laevis)
Fresh
8-day
NR-ZERO = 0.642
mg AI/L
0, 0.64 mg/L
Renewal, Nominal,
Solvent: DMSO
Mortality
{Schriks, 2006,
938764@@autho
r-year}
Unacceptable

52

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53

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
Table 2. On-topic terrestrial toxicity studies that were evaluated for HBCD
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
Terrestrial Vegetation

134237-52-8
Corn (Zea
mays)
Hydroponic
3-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots,
Radical relative
intensity in shoots
{Wu, 2012,
1927583@@au
thor-year}
High
1927583
134237-52-8
Corn (Zea
mays)
Hydroponic
7-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-52-8
Corn (Zea
mays)
Hydroponic
12-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-52-8
Corn (Zea
mays)
Hydroponic
24-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots,
Radical relative
intensity in shoots;
Histone H2AX
mRNA: Relative y-
H2AX level in
shoots
{Wu, 2012,
1927583@@au
thor-year}
High

54

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-52-8
Corn (Zea
mays)
Hydroponic
72-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-52-8
Corn (Zea
mays)
Hydroponic
96-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-52-8
Corn (Zea
mays)
Hydroponic
7-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-52-8
Corn (Zea
mays)
Hydroponic
12-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-52-8
Corn (Zea
mays)
Hydroponic
72-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

55

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-52-8
Corn (Zea
mays)
Hydroponic
96-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in shoots;
Growth:
Inhibition of seed
germination;
Growth:
Inhibition of root
biomass; Growth:
Inhibition of shoot
biomass; Growth:
Root elongation;
Growth:
Shoot elongation
{Wu, 2012,
1927583@@au
thor-year}
High

134237-52-8
Corn (Zea
mays)
Hydroponic
3-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-52-8
Corn (Zea
mays)
Hydroponic
24-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Histone H2AX
mRNA:
Relative y-H2AX
level in roots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-51-7
Corn (Zea
mays)
Hydroponic
3-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

56

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-51-7
Corn (Zea
mays)
Hydroponic
7-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots,
Radical relative
intensity in shoots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-51-7
Corn (Zea
mays)
Hydroponic
12-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots,
Radical relative
intensity in shoots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-51-7
Corn (Zea
mays)
Hydroponic
24-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots,
Radical relative
intensity in shoots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

57

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-51-7
Corn (Zea
mays)
Hydroponic
72-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-51-7
Corn (Zea
mays)
Hydroponic
96-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-51-7
Corn (Zea
mays)
Hydroponic
3-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-51-7
Corn (Zea
mays)
Hydroponic
72-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

58

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-51-7
Corn (Zea
mays)
Hydroponic
96-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in shoots;
Growth:
Inhibition of seed
germination;
Growth:
Inhibition of root
biomass; Growth:
Inhibition of shoot
biomass; Growth:
Root elongation;
Growth:
Shoot elongation
{Wu, 2012,
1927583@@au
thor-year}
High

134237-50-6
Corn (Zea
mays)
Hydroponic
3-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots,
Radical relative
intensity in shoots;
{Wu, 2012,
1927583@@au
thor-year}
High

134237-50-6
Corn (Zea
mays)
Hydroponic
7-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots,
Radical relative
intensity in shoots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

59

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-50-6
Corn (Zea
mays)
Hydroponic
12-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots,
Radical relative
intensity in shoots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-50-6
Corn (Zea
mays)
Hydroponic
24-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots,
Radical relative
intensity in shoots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-50-6
Corn (Zea
mays)
Hydroponic
72-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots,
Radical relative
intensity in shoots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots
{Wu, 2012,
1927583@@au
thor-year}
High

60

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-50-6
Corn (Zea
mays)
Hydroponic
96-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in roots;
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-50-6
Corn (Zea
mays)
Hydroponic
96-hour
LOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Reactive oxygen
species:
Radical relative
intensity in shoots;
Growth:
Inhibition of seed
germination;
Growth:
Inhibition of root
biomass; Growth:
Inhibition of shoot
biomass; Growth:
Root elongation;
Growth:
Shoot elongation
{Wu, 2012,
1927583@@au
thor-year}
High

134237-50-6
Corn (Zea
mays)
Hydroponic
3-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Histone H2AX
mRNA:
Relative y-H2AX
level in roots,
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

134237-50-6
Corn (Zea
mays)
Hydroponic
72-hour
NOAEL = 0.002
mg/L
0, 0.002 mg/L
Hydroponic solution
application, Nominal
Histone H2AX
mRNA:
Relative y-H2AX
level in shoots
{Wu, 2012,
1927583@@au
thor-year}
High

25637-99-4
Corn (Zea
mays)
Filter paper
4-day
LOAEL = 0.002
mg/L
0, 0.002,0.005,
0.01,0.02,0.05
mg/L
Renewal, Nominal,
Solvent: Methanol
Growth: Root
biomass; Growth:
Root length;
Growth: Shoot
biomass;
Germination
{Wu, 2016,
3350472@@au
thor-year}
High
3350472
61

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
25637-99-4
Corn (Zea
mays)
Filter paper
4-day
NOAEL = 0.002
mg/L;
LOAEL = 0.005
mg/L
0, 0.002,0.005,
0.01,0.02,0.05
mg/L
Renewal, Nominal,
Solvent: Methanol
Growth: Shoot
length; Histone
H2AX mRNA: Root
{Wu, 2016,
3350472@@au
thor-year}
High

25637-99-4
Corn (Zea
mays)
Filter paper
4-day
NOAEL = 0.005
mg/L;
LOAEL = 0.01
mg/L
0, 0.002,0.005,
0.01,0.02,0.05
mg/L
Renewal, Nominal,
Solvent: Methanol
Histone H2AX
mRNA: Shoot
{Wu, 2016,
3350472@@au
thor-year}
High
134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
1-week
BCF (root) =0.550
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber,
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
3350492
134237-52-8
Bread Wheat
(.Triticum
aestivum)
Natural soil
1-week
BCF (stem) =
0.100
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
1-week
BCF (leal) = 0.157
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
2-week
BCF (root) = 0.961
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
2-week
BCF (stem) =
0.203
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
2-week
BCF (leaf) = 0.259
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
62

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
3-week
BCF (root) = 1.27
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber,
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
3-week
BCF (stem) =
0.284
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-52-8
Bread Wheat
(.Triticum
aestivum)
Natural soil
3-week
BCF (leaf) = 0.473
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
4-week
BCF (root) = 1.99
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-52-8
Bread Wheat
(.Triticum
aestivum)
Natural soil
4-week
BCF (stem) =
0.472
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
4-week
BCF (leaf) = 0.755
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
1-week
BCF (root) = 1.10
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
1-week
BCF (stem) =
0.231
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
63

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
1-week
BCF (leaf) = 0.134
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
2-week
BCF (root) = 1.36
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-51-7
Bread Wheat
(.Triticum
aestivum)
Natural soil
2-week
BCF (stem) =
0.315
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
2-week
BCF (leaf) = 0.175
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-51-7
Bread Wheat
(.Triticum
aestivum)
Natural soil
3-week
BCF (root) = 2.07
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber,
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
3-week
BCF (stem) =
0.514
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
3-week
BCF (leaf) = 0.335
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
4-week
BCF (root) = 3.08
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
64

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
4-week
BCF (stem) =
0.842
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
4-week
BCF (leaf) =
0.604
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-50-6
Bread Wheat
(.Triticum
aestivum)
Natural soil
1-week
BCF (root) = 1.28
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
1-week
BCF (stem) =
0.286
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-50-6
Bread Wheat
(.Triticum
aestivum)
Natural soil
1-week
BCF (leaf) = 0.141
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
2-week
BCF (root) = 1.63
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
2-week
BCF (stem) =
0.405
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
2-week
BCF (leaf) = 0.225
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
65

-------
PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
3-week
BCF (root) = 2.13
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
3-week
BCF (stem) =
0.606
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-50-6
Bread Wheat
(.Triticum
aestivum)
Natural soil
3-week
BCF (leaf) = 0.337
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
4-week
BCF (root) = 3.21
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-50-6
Bread Wheat
(.Triticum
aestivum)
Natural soil
4-week
BCF (stem) =
0.880
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
4-week
BCF (leaf) = 0.663
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Bioconcentration
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
1-week
TF = 0.177
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High
134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
2-week
TF = 0.206
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber,
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High
66

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
3-week
TF = 0.203
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-52-8
Bread Wheat
(Triticum
aestivum)
Natural soil
4-week
TF = 0.216
0, 0.0628
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-51-7
Bread Wheat
(.Triticum
aestivum)
Natural soil
1-week
TF = 0.202
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber,
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
2-week
TF = 0.224
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-51-7
Bread Wheat
(.Triticum
aestivum)
Natural soil
3-week
TF = 0.242
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-51-7
Bread Wheat
(Triticum
aestivum)
Natural soil
4-week
TF = 0.264
0, 0.0908
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
1-week
TF = 0.218
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
2-week
TF = 0.244
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High

67

-------
PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
3-week
TF = 0.280
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High

134237-50-6
Bread Wheat
(Triticum
aestivum)
Natural soil
4-week
TF = 0.269
0, 0.0984
mg/kg dry soil
Multiple routes within
environmental
exposure chamber
Measured, Solvent:
Methylene chloride
Residue;
Translocation factor
(TF: [stem]/ [root])
{Zhu, 2016,
3350492@@au
thor-year}
High
Terrestrial Invertebrates

3194-55-6
Earthworm
(Eisenia fetida)
Artificial
soil
0-4-day
NOAEL = 400 mg
Al/kg
0, 0, 50,100,
200, 400 mg/kg
dry soil
Static, Nominal,
Solvent: Acetone
Growth: rate
{Shi, 2015,
2965902@@au
thor-year}
High
2965902
3194-55-6
Earthworm
(Eisenia fetida)
Artificial
soil
4-7-day
NOAEL = 400 mg
Al/kg
0, 0, 50,100,
200, 400 mg/kg
dry soil
Static, Nominal,
Solvent: Acetone
Growth: rate
{Shi, 2015,
2965902@@au
thor-year}
High
3194-55-6
Earthworm
(.Eisenia fetida)
Artificial
soil
7-10-day
NOAEL = 400 mg
Al/kg
0, 0, 50,100,
200, 400 mg/kg
dry soil
Static, Nominal,
Solvent: Acetone
Growth: rate
{Shi, 2015,
2965902@@au
thor-year}
High
3194-55-6
Earthworm
(.Eisenia fetida)
Artificial
soil
10-14-day
NOAEL = 400 mg
Al/kg
0, 0, 50,100,
200, 400 mg/kg
dry soil
Static, Nominal,
Solvent: Acetone
Growth: rate
{Shi, 2015,
2965902@@au
thor-year}
High
3194-55-6
Earthworm
(Eisenia fetida)
Artificial
soil
14-day
NOAEL = 400 mg
Al/kg
0, 0, 50,100,
200, 400 mg/kg
dry soil
Static, Nominal,
Solvent: Acetone
Growth: rate;
Catalase mRNA
{Shi, 2015,
2965902@@au
thor-year}
High
3194-55-6
Earthworm
(Eisenia fetida)
Artificial
soil
14-day
NOAEL = 200 mg
Al/kg; LOAEL =
400 mg Al/kg
0, 0, 50,100,
200, 400 mg/kg
dry soil
Static, Nominal,
Solvent: Acetone
Superoxide
dismutase mRNA;
HSP70 mRNA
{Shi, 2015,
2965902@@au
thor-year}
High
134237-52-8
Earthworm
(Eisenia fetida)
Natural soil
21-day
BAF = 3.77
0, 0.172 mg/g
dry soil
Static, Measured,
Solvent: Unspecified
Residue;
Bioaccumulation
{Li, 2016,
3350510@@au
thor-year}
Low
3350510
134237-52-8
Earthworm
(Metaphire
guillelmi)
Natural soil
21-day
BAF = 1.16
0, 0.172 mg/g
dry soil
Static, Measured,
Solvent: Unspecified
Residue;
Bioaccumulation
{Li, 2016,
3350510@@au
thor-year}
Low
134237-51-7
Earthworm
(Eisenia fetida)
Natural soil
21-day
BAF = 2.28
0, 0.156 mg/g
dry soil
Static, Measured,
Solvent: Unspecified
Residue;
Bioaccumulation
{Li, 2016,
3350510@@au
thor-year}
Low
134237-51-7
Earthworm
(Metaphire
guillelmi)
Natural soil
21-day
BAF = 2.81
0, 0.156 mg/g
dry soil
Static, Measured,
Solvent: Unspecified
Residue;
Bioaccumulation
{Li, 2016,
3350510@@au
thor-year}
Low
68

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PEER REVIEW DRAFT- DO NOT CITE OR QUOTE
CAS RN
Test Species
Media
Duration
End-
point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-50-6
Earthworm
(Eisenia fetida)
Natural soil
21-day
BAF = 21.8
0, 0.186 mg/g
dry soil
Static, Measured,
Solvent: Unspecified
Residue;
Bioaccumulation
{Li, 2016,
3350510@@au
thor-year}
Low

134237-50-6
Earthworm
(Metaphire
guillelmi)
Natural soil
21-day
BAF = 6.21
0, 0.186 mg/g
dry soil
Static, Measured,
Solvent: Unspecified
Residue;
Bioaccumulation
{Li, 2016,
3350510@@au
thor-year}
Low

Terrestrial Vertebrates

134237-50-6
Domestic
chicken
(Gallus
domesticus)
Culture of
embryonic
hepatocytes
24-hour
NOAEL = 0.06
mg/L;
LOAEL = 0.6
mg/L
0, 0, 0.006,
0.06,0.6, 1.9,
6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Cytochrome P450
2H1 mRNA; UGT-
1A9; Fatty acid-
binding protein 10-
A, liver basic
mRNA; Cyto-
chrome P450 3A37
mRNA
{Crump, 2008,
14081 ll@@au
thor-year}
High
1408111
134237-50-6
Domestic
chicken
('Gallus
domesticus)
Culture of
embryonic
hepatocytes
36-hour
NOAEL = 0.006
mg/L;
LOAEL = 0.06
mg/L
0, 0, 0.006,
0.06,0.6, 1.9,
6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Cytochrome P450
2H1 mRNA
{Crump, 2008,
14081 ll@@au
thor-year}
High

134237-50-6
Domestic
chicken
(Gallus
domesticus)
Culture of
embryonic
hepatocytes
36-hour
NOAEL = 0.06
mg/L;
LOAEL = 0.6
mg/L
0, 00.006, 0.06,
0.6, 1.9,6.4
mg/L
In vitro, Nominal,
Solvent: DMSO
UGT- 1A9; Fatty
acid-binding protein
10-A, liver basic
mRNA; Cyto-
chrome P450 3A37
mRNA
{Crump, 2008,
14081 ll@@au
thor-year}
High

134237-50-6
Domestic
chicken
(Gallus
domesticus)
Culture of
embryonic
hepatocytes
24-hour
NOAEL = 6.4
mg/L
0, 0, 0.006,
0.06,0.6, 1.9,
6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Xenobiotic-sensing
orphan nuclear
receptor (CXR)
mRNA
{Crump, 2008,
14081 ll@@au
thor-year}
High

134237-50-6
Domestic
chicken
(Gallus
domesticus)
Culture of
embryonic
hepatocytes
24-hour
NOAEL = 1.9
mg/L;
LOAEL = 6.4
mg/L
0, 0, 0.006,
0.06,0.6, 1.9,
6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Transthyretin (pre-
albumin, amyloid-
osis type I) mRNA;
Thyroid hormone
responsive spot 14
alpha mRNA
{Crump, 2008,
14081 ll@@au
thor-year}
High

134237-50-6
Domestic
chicken
(Gallus
domesticus)
Culture of
embryonic
hepatocytes
36-hour
NOAEL = 6.4
mg/L
0, 0, 0.006,
0.06,0.6, 1.9,
6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Trans- thyretin (pre-
albumin, amyloid-
osis type I) mRNA
{Crump, 2008,
14081 ll@@au
thor-year}
High

69

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Media
Duration
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point
Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-50-6
Domestic
chicken
(Gallus
domesticus)
Culture of
embryonic
hepatocytes
36-hour
NOAEL = 1.9
mg/L;
LOAEL = 6.4
mg/L
0, 0, 0.006,
0.06,0.6, 1.9,
6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Thyroid hormone
responsive spot 14
alpha mRNA
{Crump, 2008,
14081 ll@@au
thor-year}
High

134237-50-6
HBCD-
Technical
Mixture
Domestic
chicken
('Gallus
domesticus)
Culture of
embryonic
hepatocytes
24-hour
NOAEL = 0.06
mg/L;
LOAEL = 0.6
mg/L
0, 0, 0.006,
0.06,0.6, 1.9,
6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Thyroid hormone
responsive spot 14
alpha mRNA; Cyto-
chrome P450 2H1
mRNA; Cyto-
chrome P450 3A37
mRNA
{Crump, 2008,
14081 ll@@au
thor-year}
High
134237-50-6
HBCD-
Technical
Mixture
Domestic
chicken
(Gallus
domesticus)
Culture of
embryonic
hepatocytes
36-hour
NOAEL = 0.06
mg/L;
LOAEL = 0.6
mg/L
0, 0, 0.006,
0.06,0.6, 1.9,
6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Thyroid hormone
responsive spot 14
alpha mRNA; Fatty
acid-binding protein
10-A, liver basic
mRNA; Cyto-
chrome P450 2H1
mRNA; Cyto-
chrome P450 3A37
mRNA
{Crump, 2008,
14081 ll@@au
thor-year}
High
134237-50-6
HBCD-
Technical
Mixture
Domestic
chicken
(Gallus
domesticus)
Culture of
embryonic
hepatocytes
24-hour
NOAEL = 6.4
mg/L
0, 0, 0.006,
0.06,0.6, 1.9,
6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
UGT- 1A9
{Crump, 2008,
14081 ll@@au
thor-year}
High
134237-50-6
HBCD-
Technical
Mixture
Domestic
chicken
(Gallus
domesticus)
Culture of
embryonic
hepatocytes
36-hour
NOAEL = 6.4
mg/L
0, 0, 0.006,
0.06,0.6, 1.9,
6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
UGT- 1A9; Trans-
thyretin (pre-
albumin, amyloid-
osis type I) mRNA
{Crump, 2008,
14081 ll@@au
thor-year}
High
134237-50-6
HBCD-
Technical
Mixture
Domestic
chicken
(Gallus
domesticus)
Culture of
embryonic
hepatocytes
24-hour
NOAEL = 1.9
mg/L;
LOAEL = 6.4
mg/L
0, 0, 0.006,
0.06,0.6, 1.9,
6.4 mg/L
In vitro, Nominal,
Solvent: DMSO
Transthyretin (pre-
albumin, amyloid-
osis type I) mRNA;
Fatty acid-binding
protein 10-A, liver
basic mRNA
{Crump, 2008,
14081 ll@@au
thor-year}
High
134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
1-day
NOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Abdominal fat
{Fournier,
2012,
1927629@@au
thor-year}
High
1927629
70

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Conc(s)
Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
4-day
NOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Abdominal fat
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
('Gallus
domesticus)
Diet
8-day
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Abdominal fat
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
('Gallus
domesticus)
Diet
11-day
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Abdominal fat
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
16-day
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Abdominal fat
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
21-day
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Abdominal fat
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
(iGallus
domesticus)
Diet
21-day, 1
day
depuration
LOAEL = 0.001
mg A/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Abdominal fat
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
21-day, 3
days
depuration
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Abdominal fat
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
21-day, 8
days
depuration
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Abdominal fat
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
('Gallus
domesticus)
Diet
21-day, 18
days
depuration
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Abdominal fat
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
('Gallus
domesticus)
Diet
1-day
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Liver
{Fournier,
2012,
1927629@@au
thor-year}
High

71

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Effect(s)
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Data
Quality
Ratings
HERO
ID.
134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
4-day
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Liver
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
('Gallus
domesticus)
Diet
8-day
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Liver
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
('Gallus
domesticus)
Diet
11-day
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Liver
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
16-day
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Liver
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
21-day
LOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Liver
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
(iGallus
domesticus)
Diet
21-day, 1
day
depuration
NOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Liver
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
21-day, 3
days
depuration
NOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Liver
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
21-day, 8
days
depuration
NOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Liver
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
('Gallus
domesticus)
Diet
21-day, 18
days
depuration
NOAEL = 0.001
mg Al/kg food
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioaccumulation in
Liver
{Fournier,
2012,
1927629@@au
thor-year}
High

134237-52-8
Domestic
chicken
('Gallus
domesticus)
Diet
21-day
BCF (egg yolk) =
0.4
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioconcentration
{Fournier,
2012,
1927629@@au
thor-year}
High

72

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point
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Test Analysis
Effect(s)
References
Data
Quality
Ratings
HERO
ID.
134237-52-8
Domestic
chicken
(Gallus
domesticus)
Diet
21-day
BCF (liver) = 0.3
0, 0.001 mg/kg
food
Food, Nominal,
Solvent: Rapeseed oil
Residue:
Bioconcentration
{Fournier,
2012,
1927629@@au
thor-year}
High

25637-99-4
American
Kestrel (Falco
sparverius)
Diet
4 weeks
prior to
pairing,
continuing
through
incubation
until 2 days
prior to
hatch
LOAEL (males
and females) =
0.51 mg Al/kg
food
0, 0.51 mg/kg-
bw/day
Food, Nominal,
Solvent: Safflower oil
Decreased activity,
general: measured
during courtship,
measured at 5 days
after pairing
{Marteinson,
2012,
1927590@@au
thor-year}
High
1927590
25637-99-4
American
Kestrel (Falco
sparverius)
Diet
4 weeks
prior to
pairing,
continuing
through
incubation
until 2 days
prior to
hatch
LOAEL (males) =
0.51 mg/kg-bw/day
0, 0.51 mg/kg-
bw/day
Food, Nominal,
Solvent: Safflower oil
Decreased activity,
general and flying
measured during
brood-rearing;
Courtship behavior:
Reduced
vocalizations, effect
observed throughout
courtship; Pair-
bonding nesting
behavior: Reduced
Displays; Care of
young, nest
attentiveness:
Reduced frequency
of entry into nest-
box and Decreased
food retrieval
{Marteinson,
2012,
1927590@@au
thor-year}
High
73

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Effect(s)
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Ratings
HERO
ID.
25637-99-4
American
Diet
4 weeks
LOAEL (females)
0, 0.51 mg/kg-
Food, Nominal,
Courtship behavior:
{Marteinson,
High


Kestrel (Falco

prior to
= 0.51 mg/kg-
bw/day
Solvent: Safflower oil
Reduced
2012,



sparverius)

pairing,
continuing
through
incubation
until 2 days
prior to
hatch
bw/day


vocalizations, effect
observed only at 5
days after pairing,
Reduced courtship
displays, effect
observed at 5 days
after pairing; Pair-
bonding nesting
behavior: Increased
displays; Care of
young, nest
attentiveness:
Increased frequency
of entry into nest-
box and Increased
food retrieval
1927590@@au
thor-year}


25637-99-4
American
Diet
4 weeks
LOAEL = 0.51
0, 0.51 mg/kg-
Food, Nominal,
Reduced mass of
{Marteinson,
High


Kestrel {Falco

prior to
mg/kg-bw/day
bw/day
Solvent: Safflower oil
first egg; Care of
2012,



sparverius)

pairing,
continuing
through
incubation
until 2 days
prior to
hatch



young, nest
attentiveness:
Incubation nest
temperature
1927590@@au
thor-year}


74

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HERO
ID.
25637-99-4
American
Kestrel (Falco
sparverius)
Diet
75 days: 3
weeks prior
to pairing,
continuing
through
incubation
until first
chick
hatched
LOAEL = 0.51 mg
Al/kg food
0, 0.51 mg/kg-
bw/day
Food, Nominal,
Solvent: Safflower oil
Residue:
Accumulation in
Eggs; Reproductive:
Decreased time to
first egg laid after
pairing, decreased
clutch size,
decreased egg
volume per clutch,
decreased egg
volume per pair,
decreased egg mass
per clutch,
Decreased egg mass
at mid-incubation,
Increased egg
weight loss at mid-
incubation
{Fernie, 2011,
1401837@@au
thor-year}
High
1401837
25637-99-4
American
Kestrel {Falco
sparverius)
Diet
75 days: 3
weeks prior
to pairing,
continuing
through
incubation
until first
chick
hatched
NOAEL = 0.51 mg
Al/kg food
0, 0.51 mg/kg-
bw/day
Food, Nominal,
Solvent: Safflower oil
Lipid concentration
in eggs; Repro-
ductive: Egg shell
thickness, Overall
hatching success
(number of
hatchlings), Overall
reproductive success
(number of
fledglings per
brood/number of
eggs per female),
Fertility (percentage
fertile eggs laid per
female), Elatching
success (percentage
hatchlings of fertile
eggs per female),
Fledgling success
(percentage
fledglings of
hatchlings per
female)
{Fernie, 2011,
1401837@@au
thor-year}
High

75

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HERO
ID.

American
kestrel (F.
sparverius)
Diet
exposed
21-day
LOAEL
3.27 ng/g ww
(low exposure)
Food, Nominal,
Solvent: Safflower oil
Reproduction
{Marteinson,
2010,
1927669@@au
thor-year}
High
1927669

American
kestrel (F.
sparverius)
Diet
exposed
21-day
LOAEL
15.61 ng/g ww
(high exposure)
Food, Nominal,
Solvent: Safflower oil
Reproduction
{Marteinson,
2010,
1927669@@au
thor-year}
High

American
kestrel (F.
sparverius)
Diet
exposed
21-day
LOAEL
0.51 mg/kg-day
HBCD dissolved in
safflower oil was
injected into the brains
of dead cockerels
daily; kestrels fed from
the cockerels ad
libitum and received a
dose of approximately
0.51 mg/kg-day.
Increased testes
weight in unpaired
males
{Marteinson,
2011,
1927624@@au
thor-year}
High
1927624
76

-------