OPPTS Harmonized Test Guidelines - Master List
Last Updated July 2009
The OPPTS harmonized guidelines are organized in the following series:
Series No.
Series Name
Docket ID No.
Last
Changed
810
Product Performance Test Guidelines
EPA-HQ-OPPT-2009-0150
Aug-2004
830
Product Properties Test Guidelines
EPA-HQ-OPPT-2009-0151
Nov-2008
835
Fate, Transport and Transformation Test Guidelines
EPA-HQ-OPPT-2009-0152
Nov-2008
840
Spray Drift Test Guidelines
EPA-HQ-OPPT-2009-0153
Mar-1998
850
Ecological Effects Test Guidelines
EPA-HQ-OPPT-2009-0154
Apr-1996
860
Residue Chemistry Test Guidelines
EPA-HQ-OPPT-2009-0155
Nov-2008
870
Health Effects Test Guidelines
EPA-HQ-OPPT-2009-0156
Mar-2003
875
Occupational and Residential Exposure Test Guidelines
EPA-HQ-OPPT-2009-0157
Feb-1996
880
Biochemicals Test Guidelines
EPA-HQ-OPPT-2009-0158
Feb-1996
885
Microbial Pesticide Test Guidelines
EPA-HQ-OPPT-2009-0159
Feb-1996
890
Endocrine Distruptor Screening Program Test Guidelines
EPA-HQ-OPPT-2009-xxxx
Jul-2009
FINAL Guidelines
The Final guidelines can be be accessed through http://www.epa.gov/opptsfrs/home/guidelin.htm, as well as through www.Regulations.gov using the
Docket ID Number provided in the table.
DRAFT Guidelines
Please note, those guidelines labeled as "Public Draft" are listed because they were issued in draft for public review and comment, but are not yet
available in final form. Although you may consult these guidelines, please check with the appropriate office before you use a draft guideline to
generate data for submission to EPA under FIFRA, FFDCA or TSCA.
Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

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OPPTS Harmonized Test Guideline - Series Index
Series 810—Product Performance Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0150 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub
Number
Date
Issued
OPPT OPP OECD
Final 810 Test Guidelines
Group A—General.
810.1000
Overview, Definitions, and General Considerations
none
90-1,
90-3,
90-30
none
712-C-98-001
Mar-98







Group B—[Reserved].
810.20001 [Reserved]
Group C—Invertebrate Control Agent Product Performance Test Guidelines.
810.3000
General Considerations for Efficacy of Invertebrate Control Agents
none
95-1
none
712-C-98-409
Mar-98
810.3100
Soil Treatments for Imported Fire Ants
none
95-3
none
712-C-98-410
Mar-98
810.3200
Livestock, Poultry, Fur- and Wool-bearing Animal Treatments
none
95-8
none
712-C-98-414
Mar-98
810.3300
Treatments to Control Pests of Humans and Pets
none
95-9,
95-30,
none
712-C-98-411
Mar-98
810.3400
Mosquito, Black Fly, and Biting Midge (Sand Fly) Treatments
none
95-10
none
712-C-98-419
Mar-98
810.3500
Premises Treatments
none
95-11,
95-30
none
712-C-98-413
Mar-98
810.3600
Structural Treatments
none
95-12
none
712-C-98-424
Mar-98
810.3700
[Reserved]





810.3800
Methods for Efficacy Testing of Termite Baits
none

none
712-C-04-371
Aug-04







DRAFT 810 Test Guidelines
Please note, those guidelines labeled as "Public Draft" are are not yet available in final form. Although you may consult these guidelines, please check
with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA or TSCA.
810.3700
Insect Repellents for Human Skin and Outdoor Premises
none
95-9,
95-10
none
712-C-99-369
Dec-99







Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

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OPPTS Harmonized Test Guideline - Series Index
Series 830—Product Properties Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0151 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub
No.
Date
Issued
OPPT OPP OECD
Final 830 Test Guidelines
830.1000
Background for Product Properties Test Guidelines
none
60-1,
60-2,
60-3
none
712-C-96-310
Mar-98







Group A—Product Identity, Composition, and Analysis Test Guidelines.
830.1550
Product Identity and Composition
none
158.155
none
712-C-96-006
Aug-96
830.1600
Description of Materials Used to Produce the Product
none
158.160
none
712-C-96-007
Aug-96
830.1620
Description of Production Process
none
158.162
104
712-C-96-008
Aug-96
830.1650
Description of Formulation Process
none
158.165
104
712-C-96-009
Aug-96
830.1670
Discussion of Formation of Impurities
none
158.167
104
712-C-96-010
Aug-96
830.1700
Preliminary Analysis
none
158.170
104
712-C-96-011
Aug-96
830.1750
Certified Limits
none
158.175
104
712-C-96-012
Aug-96
830.1800
Enforcement Analytical Method
none
158.180
104
712-C-96-013
Aug-96
830.1900
Submittal of Samples
none
64-1
104
712-F-08-002
Nov-08
Group B—Physical/Chemical Properties Test Guidelines.
830.6302
Color
none
63-2
none
712-C-96-019
Aug-96
830.6303
Physical State
none
63-3
none
712-C-96-020
Aug-96
830.6304
Odor
none
63-4
none
712-C-96-021
Aug-96
830.6313
Stability to Normal and Elevated Temperatures, Metals, and Metal
Ions
none
63-13
none
712-C-96-022
Aug-96
830.6314
Oxidation/Reduction: Chemical Incompatabiity
none
63-14
none
712-C-96-023
Aug-96
830.6315
Flammability
none
63-15
none
712-C-96-024
Aug-96
830.6316
Explodability
none
63-16
none
712-C-96-025
Aug-96
830.6317
Storage Stability
none
63-17
113
712-C-96-026
Jun-02
830.6319
Miscibility
none
63-19
none
712-C-96-027
Aug-96
830.6320
Corrosion Characteristics
none
63-20
none
712-C-96-028
Aug-96
830.6321
Dielectric Breakdown Voltage
none
63-21
none
712-C-96-029
Aug-96
830.7000
PH
796.1450
63-12
none
712-C-96-030
Aug-96
830.7050
UV/Visible Absorption
796.1050
none
101
712-C-96-031
Aug-96
830.7100
Viscosity
none
63-18
114
712-C-96-032
Aug-96
830.7200
Melting Point/Melting Range
796.1300
63-5
102
712-C-96-033
Mar-98
830.7220
Boiling Point/Boiling Range
796.1220
63-6
103
712-C-96-034
Aug-96
Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

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OPPTS Harmonized Test Guideline - Series Index
Series 830—Product Properties Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0151 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub
No.
Date
Issued
OPPT
OPP
OECD
830.7300
Density/Relative Density/Bulk Density
796.1150
63-7
109
712-C-96-035
Jun-02
830.7370
Dissociation Constants in Water
796.1370
63-10
112
712-C-96-036
Aug-96
830.7520
Particle Size, Fiber Length, and Diameter Distribution
796.1520
none
110
712-C-96-037
Aug-96
830.7550
Partition Coefficient (n-octanol/water), Shake Flask Method
796.1550
63-11
107
712-C-96-038
Aug-96
830.7560
Partition Coefficient (n-octanol/water), Generator Column Method
796.1720
63-11
none
712-C-96-039
Aug-96
830.7570
Partition Coefficient (n-octanol/water), Estimation by Liquid
Chromatography
796.1570
63-11
117
712-C-96-040
Aug-96
830.7840
Water Solubility: Column Elution Method; Shake Flask Method
796.1840
63-8
105
712-C-96-041
Mar-98
830.7860
Water Solubility, Generator Column Method
796.1860
63-8
none
712-C-96-042
Mar-98
830.7950
Vapor Pressure
796.1950
63-9
104
712-C-96-043
Aug-96







DRAFT 830 Test Guidelines
Please note, those guidelines labeled as "Public Draft" are are not yet available in final form. Although you may consult these guidelines, please check
with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA or TSCA.

None.












Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 835—Fate, Transport and Transformation Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0152 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Final 835 Test Guidelines
835.0001
Principles and Strategies Related to Biodegradation Testing of
Organic Chemicals under the Toxic Substances Control Act (TSCA)
none
none
none
712-C-08-008
Nov-08







Group A—Laboratory Transport Test Guidelines.
835.1110
Activated Sludge Sorption Isotherm
none
none
none
712-C-98-298
Jan-98
835.1210
Soil Thin Layer Chromatography
796.2700
none
none
712-C-98-047
Jan-98
835.1220
Sediment and Soil Adsorption/Desorption Isotherm
796.2750
none
106
712-C-98-048
Jan-98
835.1230
Adsorption/Desorption (Batch Equilibrium)
none
163-1
106
712-C-08-009
Nov-08
835.1240
Leaching Studies
none
163-1
312
712-C-08-010
Nov-08
835.1410
Laboratory Volatility
none
163-2
none
712-C-08-011
Nov-08







Group B—Laboratory Abiotic Transformation Test Guidelines.
835.2110
Hydrolysis as a Function of pH [SUPERSEDED BY 835.2120]
796.3500
none
111
712-C-98-057
Jan-98
835.2120
Hydrolysis [SUPERSEDES 835.2110]
none
161-1
111
712-C-08-012
Nov-08
835.2130
Hydrolysis as a Function of pH and Temperature
796.3510
none
none
712-C-98-059
Jan-98
835.2210
Direct Photolysis Rate in Water by Sunlight
796.3700
none
none
712-C-98-060
Jan-98
835.2240
Photodegradation in Water
none
161-2
none
712-C-08-013
Nov-08
835.2310
Maximum Direct Photolysis Rate in Air from UV/Visible Spectroscopy
796.3800
none
none
712-C-98-066
Jan-98
835.2370
Photodegradation in Air
none
161-4
none
712-C-08-014
Nov-08
835.2410
Photodegradation in Soil
none
161-3
none
712-C-08-015
Nov-08







Group C—Laboratory Biological Transformation Test Guidelines.
835.3100
Aerobic Aquatic Biodegradation
796.3100
none
none
712-C-98-075
Jan-98
835.3110
Ready Biodegradability
796.3180,
796.3200,
796.3220,
796.3240,
796.3260.
none
301
712-C-98-076
Jan-98
835.3120
Sealed-Vessel Carbon Dioxide Production Test [SUPERSEDED BY
31401
none
none
none
712-C-98-311
Jan-98
Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

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OPPTS Harmonized Test Guideline - Series Index
Series 835—Fate, Transport and Transformation Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0152 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT
OPP
OECD
835.3140
Ready Biodegradability - C02 in Sealed Vessels (Headspace Test)
[SUPERSEDES 3120]
none
none
310
712-C-08-001
Nov-08
835.3160
Biodegradability in Sea Water
none
none
306
712-C-98-351
Jan-98
835.3170
Shake Flask Die-Away Test
none
none
none
712-C-98-297
Jan-98
835.3180
Sediment/Water Microcosm Biogradation Test
none
none
none
712-C-98-083
Jan-98
835.3200
Zahn-Wellens/EMPA Test
796.3360
none
302B
712-C-98-084
Jan-98
835.3210
Modified SCAS Test
796.3340
none
302A
712-C-98-085
Jan-98
835.3220
Porous Pot Test
none
none
none
712-C-98-301
Jan-98
835.3300
Soil Biodegradation
796.3400
none
304A
712-C-98-088
Jan-98
835.3400
Anaerobic Biodegradability of Organic Chemicals
796.3140
none
none
712-C-98-090
Jan-98







Group D—[Reserved].
I
Group E—Transformation Chemical-Specific Test Guidelines.
835.5045
Modified SCAS Test for Insoluble and Volatile Chemicals
795.4500
none
none
712-C-98-097
Jan-98
835.5154
Anaerobic Biodegradation in the Subsurface
795.5400
none
none
712-C-98-098
Jan-98
835.5270
Indirect Photolysis Screening Test: Sunlight Photolysis in Waters
Containing Dissolved Humic Substances
795.7000
none
none
712-C-98-099
Jan-98







Group F—[Reserved].
I
Group G—Ground Water Monitoring Test Guidelines. [Reserved].
I
Group H—Volatility from Soil Test Guidelines.
835-8100
Field Volatility
none
163-3
none
712-C-08-024
Nov-08







DRAFT 835 Test Guidelines
Please note, those guidelines labeled as "Public Draft" are are not yet available in final form. Although you may consult these guidelines, please check
with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA or TSCA.

None.












Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

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OPPTS Harmonized Test Guideline - Series Index
Series 840—Spray Drift Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0153 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Final 840 Test Guidelines
840.1000
Background for Pesticide Aerial Drift Evaluation
none
201-1,
201-4
none
712-C-98-319
Mar-98
840.1100
Spray Droplet Size Spectrum
none
201-1
none
712-C-98-055
Mar-98
840.1200
Spray Drift Field Deposition
none
201-1
none
712-C-98-112
Mar-98







DRAFT 840 Test Guidelines
Please note, those guidelines labeled as "Public Draft" are are not yet available in final form. Although you may consult these guidelines, please check
with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA or TSCA.

None.












Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 850—Ecological Effects Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0154 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Final 850 Test Guidelines
850.1000
[Reserved]












Group A—Aq
uatic Fauna Test Guidelines.
850.1010
[Reserved]





850.1020
[Reserved]





850.1025
[Reserved]





850.1035
[Reserved]





850.1045
[Reserved]





850.1055
[Reserved]





850.1075
[Reserved]





850.1085
[Reserved]





850.1300
[Reserved]





850.1350
[Reserved]





850.1400
[Reserved]





850.1500
[Reserved]





850.1710
[Reserved]





850.1730
[Reserved]





850.1735
[Reserved]





850.1740
[Reserved]





850.1790
[Reserved]





850.1800
[Reserved]





850.1850
[Reserved]





850.1900
[Reserved]





850.1925
[Reserved]





850.1950
[Reserved!












Group B—Terrestrial Wildlife Test Guidelines.
850.2100
[Reserved]





850.2200
[Reserved]





850.2300
[Reserved]





850.2400
[Reserved]





850.2450
[Reserved]





850.2500
[Reserved!





Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

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OPPTS Harmonized Test Guideline - Series Index
Series 850—Ecological Effects Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0154 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT
OPP
OECD







Group C—Beneficial Insects and Invertibrates Test Guidelines.
850.3020
[Reserved]





850.3030
[Reserved]





850.3040
[Reserved]












Group D—Nontarget Plants Test Guidelines
850.4000
[Reserved]





850.4025
[Reserved]





850.4100
[Reserved]





850.4150
[Reserved]





850.4200
[Reserved]





850.4225
[Reserved]





850.4230
[Reserved]





850.4250
[Reserved]





850.4300
[Reserved]





850.4400
[Reserved]





850.4450
[Reserved]





850.4600
[Reserved]





850.4800
[Reserved!












Group E—Toxicity to Microorganisms Test Guidelines.
850.5100
[Reserved]





850.5400
[Reserved!












Group F—Chemical Specific Test Guidelines.
850.6200
[Reserved]





850.6800
[Reserved]












Group G—Field Test Data Reporting Guidelines
850.7100
[Reserved!












DRAFT 850 Test Guidelines
Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

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OPPTS Harmonized Test Guideline - Series Index
Series 850—Ecological Effects Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0154 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Please note, those guidelines labeled as "Public Draft" are are not yet available in final form. Although you may consult these guidelines, please check
with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA or TSCA.
850.1000
Special Consideration for Conducting Aquatic Laboratory Studies
none
none
none
712-C-96-113
Apr-96







Group A—Aq
juatic Fauna Test Guidelines.
850.1010
Aquatic invetebrate Acute Toxicity, Test, Freshhwater Daphnids
797.1300
72-2
none
712-C-96-114
Apr-96
850.1020
Gammarid Acute Toxicity Test
795.120
none
none
712-C-96-130
Apr-96
850.1025
Oyster Acute Toxicity Test (Shell Deposition)
797.1800
72-3
none
712-C-96-115
Apr-96
850.1035
Mysid Acute Toxicity Test
797.1930
72-3
none
712-C-96-136
Apr-96
850.1045
Penaeid Acute Toxicity Test
797.1970
72-3
none
712-C-96-137
Apr-96
850.1055
Bivalve Acute Toxicity Test (Embryo Larval)
none
72-3
none
712-C-96-100
Apr-96
850.1075
Fish Acute Toxicity Test, Freshwater and Marine
797.1400
72-1,
72-3
203
712-C-96-118
Apr-96
850.1085
Fish Acute Toxicity Mitigated by Humic Acid
797.1460
none
none
712-C-96-117
Apr-96
850.1300
Daphnid Chronic Toxicity Test
797.1330
72-4
202
712-C-96-120
Apr-96
850.1350
Mysid Chronic Toxicity Test
797.1950
72-4
none
712-C-96-166
Apr-96
Document incorrectly says publication Number ends in "-120".
850.1400
Fish Early-Life Stage Toxicity Test
797.1000
72-4
210
712-C-96-121
Apr-96
850.1500
Fish Life Cycle Toxicity
none
72-5
none
712-C-96-122
Apr-96
850.1710
Oyster BCF
797.1830
72-6
none
712-C-96-127
Apr-96
850.1730
Fish BCF
797.1520
72-6,
165-4
305
712-C-96-129
Apr-96
850.1735
Whole Sediment Acute Toxicity Invertebrates, Freshwater
none
none
none
712-C-96-354
Apr-96
850.1740
Whole Sediment Acute Toxicity Invertebrates, Marine
none
none
none
712-C-96-355
Apr-96
850.1790
Chironomid Sediment Toxicity Test
795.135
none
none
712-C-96-313
Apr-96
850.1800
Tadpole/Sediment Subchronic Toxicity Test
797.1995
none
none
712-C-96-132
Apr-96
850.1850
Aquatic Food Chain Transfer
none
72-6
none
712-C-96-133
Apr-96
850.1900
Generic Freshwater Microcosm Test, Laboratory
797.3050,
797.3100
none
none
712-C-96-134
Apr-96
850.1925
Site-Specific Aquatic Microcosm Test, Laboratory
797.3100
none
none
712-C-96-173
Apr-96
850.1950
Field Testing for Aquatic Organisms
none
72-7,
165-5
none
72-C-96-135
Apr-96







Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

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OPPTS Harmonized Test Guideline - Series Index
Series 850—Ecological Effects Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0154 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Group B—Terrestrial Wildlife Test Guidelines.
850.2100
Avian Acute Oral Toxicity Test
797.2175
71-1
none
712-C-96-139
Apr-96
850.2200
Avian Dietary Toxicity Test
797.2050
71-2
205
712-C-96-140
Apr-96
850.2300
Avian Reproduction Test
797.2130,
797.2150
71-4
206
712-C-96-141
Apr-96
850.2400
Wild Mammal Acute Toxicity
none
71-3
none
712-C-96-142
Apr-96
850.2450
Terrestrial (Soil-Core) Microcosm Test
797.3775
none
none
712-C-96-143
Apr-96
850.2500
Field Testing for Terrestrial Wildlife
none
71-5
none
712-C-96-144
Apr-96







Group C—Beneficial Insects and Invertibrates Test Guidelines.
850.3020
Honey Bee Acute Contact Toxicity
none
141-1
none
712-C-96-147
Apr-96
850.3030
Honey Bee Toxicity of Residues on Foliage
none
141-2
none
712-C-96-148
Apr-96
850.3040
Field Testing for Pollinators
none
141-5
none
712-C-96-150
Apr-96







Group D—Nontarget Plants Test Guidelines
850.4000
Background—Nontarget Plant Testing
none
120-1
none
712-C-96-151
Apr-96
850.4025
Target Area Phytotoxicity
none
121-1
none
712-C-96-152
Apr-96
850.4100
Terrestrial Plant Toxicity, Tier 1 (Seedling Emergence)
none
122-1
none
712-C-96-153
Apr-96
850.4150
Terrestrial Plant Toxicity, Tier 1 (Vegetative Vigor)
none
122-1
none
712-C-96-163
Apr-96
850.4200
Seed Germination/Root Elongation Toxicity Test
797.2750
122-1
none
712-C-96-154
Apr-96
850.4225
Seedling Emergence, Tier II
797.2750
123-1
none
712-C-96-313
Apr-96
850.4230
Early Seedling Growth Toxicity Test
797.2800
123-1
none
712-C-96-347
Apr-96
850.4250
Vegetative Vigor, Tier II
797.2750
123-1
none
712-C-96-364
Apr-96
850.4300
Terrestrial Plants Field Study, Tier III
none
124-1
none
712-C-96-155
Apr-96
850.4400
Aquatic Plant Toxicity Test using Lemna spp. Tiers 1 and II
797.1160
122-2,
123-2
none
712-C-96-156
Apr-96
850.4450
Aquatic Plants Field Study, Tier III
none
124-2
none
712-C-96-157
Apr-96
850.4600
Rhizobium-Legume Toxicity
797.2900
none
none
712-C-96-158
Apr-96
850.4800
Plant Uptake and Translocation Test
797.2850
none
none
712-C-96-159
Apr-96







Group E—Toxicity to Microorganisms Test Guidelines.
850.5100
Soil Microbial Community Toxicity Test
797.3700
none
none
712-C-96-161
Apr-96
850.5400
Algal Toxicity, Tiers 1 and II
797.1050
122-2,
123-2
none
712-C-96-164
Apr-96
Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 850—Ecological Effects Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0154 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT
OPP
OECD







Group F—Chemical Specific Test Guidelines.
850.6200
Earthworm Subchronic Toxicity tTst
795.150
none
207
712-C-96-167
Apr-96
850.6800
Modified Activated Sludge, Respiration Inhibition Test for Sparingly
Soluble Chemicals
795.170
none
209
712-C-96-168
Apr-96







Group G—Field Test Data Reporting Guidelines
850.7100
Data Reporting for Environmental Chemistry Methods
none
none
none
712-C-96-348
Apr-96







Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 860—Residue Chemistry Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0155 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Final 860 Test Guidelines
860.1000
Background
none
170-1
none
712-C-96-169
Aug-96
860.1000 Supplement: Guidance on Constructing Maximum
Reasonably Balanced Diets (MRBD)



n/a
Jun-08
860.1100
Chemical Identity
none
171-2
none
712-C-96-170
Aug-96
860.1200
Directions for Use
none
171-3
none
712-C-96-171
Aug-96
860.1300
Nature of the Residue—Plants, Livestock
none
171-4a,b
none
712-C-96-172
Aug-96
860.1340
Residue Analytical Method
none
171-4c,d
none
712-C-96-174
Aug-96
860.1360
Multiresidue method
none
E
I
none
712-C-96-176
Aug-96
860.1380
Storage Stability Data
none
CD
I
none
712-C-96-177
Aug-96
Document incorrectly says publication number ends in "-95-177".
860.1400
Water, Fish, and Irrigated Crops
none
171—4f,g,h,
165-5
none
712-C-96-178
Aug-96
860.1460
Food Handling
none
171 —4i
none
712-C-96-181
Aug-96
860.1480
Meat/Milk/Poultry/Eggs
none
171 -4j
none
712-C-96-182
Aug-96
860.1500
Crop Field Trials
none
171-4k
none
712-C-96-183
Aug-96
860.1520
Processed Food/Feed
none
171-41
none
712-C-96-184
Aug-96
860.1550
Proposed Tolerances
none
171-6
none
712-C-96-186
Aug-96
860.1560
Reasonable Grounds in Support of the Petition
none
171-7
none
712-C-96-187
Aug-96
860.1650
Submittal of Analytical Reference Standards
none
171-13
none
712-F-08-001
Nov-08
860.1850
Confined Accumulation in Rotational Crops
none
165-1
none
712-C-96-188
Aug-96
860.1900
Field Accumulation in Rotational Crops
none
165-2
none
712-C-96-189
Aug-96







DRAFT 860 Test Guidelines
Please note, those guidelines labeled as "Public Draft" are are not yet available in final form. Although you may consult these guidelines, please check
with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA or TSCA.

None.












Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 870—Health Effects Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0156 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Final 870 Test Guidelines
Group A—Acute Toxicity Test Guidelines.
870.1000
Acute Toxicity Testing-Background
none
none
none
712-C-02-189
Dec-02
870.1100
Acute Oral Toxicity (AOT)
798.1175
81-1
401,420,
423,425
712-C-02-190
Dec-02

- AOT Up-And-Down Procedure (Additional Guidance & Tools)





870.1200
Acute Dermal Toxicity
798.1100
81-2
402
712-C-98-192
Aug-98
870.1300
Acute Inhalation Toxicity
798.1150
81-3
403
712-C-98-193
Aug-98
870.1350
[Reserved]





870.2400
Acute Eye Irritation
798.4500
81-4
405
712-C-98-195
Aug-98
870.2500
Acute Dermal Irritation
798.4470
81-5
404
712-C-98-196
Aug-98
870.2600
Skin Sensitization
798.4100
81-6
406
712-C-03-197
Mar-03







Group B—Subchronic Toxicity Test Guidelines.
870.3050
Repeated Dose 28-day Oral Toxicity Study in Rodents
none
none
407
712-C-00-366
Jul-00
870.3100
90-Day Oral Toxicity in Rodents
798.2650
82-1
408
712-C-98-199
Aug-98
870.3150
90-Day Oral Toxicity in Nonrodents
none
82-1
409
712-C-98-200
Aug-98
870.3200
21/28-Day Dermal Toxicity
none
82-2
410
712-C-98-201
Aug-98
870.3250
90-Day Dermal Toxicity
798.2250
82-3
411
712-C-98-202
Aug-98
870.3465
90-Day Inhalation Toxicity
798.2450
82-4
413
712-C-98-204
Aug-98
870.3500
[Reserved]





870.3550
Reproduction/Development Toxicity Screening Test
none
none
421
712-C-00-367
Jul-00
870.3600
[Reserved]





870.3650
Combined Repeated Dose Toxicity with the
Reproduction/Development Toxicity Screening Test
none
none
422
712-C-00-368
Jul-00
870.3700
Prenatal Developmental Toxicity Study
798.4900
83-3
414
712-C-98-207
Aug-98
870.3800
Reproduction and Fertility Effects
798.4700
83-4
416
712-C-98-208
Aug-98







Group C—Chronic Toxicity Test Guidelines.
870.4100
Chronic Toxicity
798.3260
83-1
452
712-C-98-210
Aug-98
870.4200
Carcinogenicity
798.3300
83-2
451
712-C-98-211
Aug-98
870.4300
Combined Chronic Toxicity/Carcinogenicity
798.3320
83-5
453
712-C-98-212
Aug-98







Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 870—Health Effects Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0156 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Group D—Genetic Toxicity Test Guidelines
870.5100
Bacterial Reverse Mutation Test
798.5100,
798.5265
84-2
471
712-C-98-247
Aug-98
870.5140
Gene Mutation in Aspergillus nidulans
798.5140
84-2
none
712-C-98-215
Aug-98
870.5195
Mouse Biochemical Specific Locus Test
798.5195
84-2
none
712-C-98-216
Aug-98
870.5200
Mouse Visible Specific Locus Test
798.5200
84-2
none
712-C-98-217
Aug-98
870.5250
Gene Mutation in Neurospora crassa
798.5250
84-2
none
712-C-98-218
Aug-98
870.5265
[Reserved]





870.5275
Sex-linked Recessive Lethal Test in Drosophila melanogaster
798.5275
84-2
477
712-C-98-220
Aug-98
870.5300
In vitro Mammalian Cell Gene Mutation Test
798.5300
84-2
476
712-C-98-221
Aug-98
870.5375
In Vitro Mammalian Chromosome Aberration Test
798.5375
84-2
473
712-C-98-223
Aug-98
870.5380
Mammalian Spermatogonia! Chromosomal Aberration Test
798.5380
84-2
483
712-C-98-224
Aug-98
870.5385
Mammalian Bone Marrow Chromosomal Aberration Test
798.5385
84-2
475
712-C-98-225
Aug-98
870.5395
Mammalian Erythrocyte Micronucleus Test
798.5395
84-2
474
712-C-98-226
Aug-98
870.5450
Rodent Dominant Lethal Assay
798.5450
84-2
478
712-C-98-227
Aug-98
870.5460
Rodent Heritable Translocation Assays
798.5460
84-2
485
712-C-98-228
Aug-98
870.5500
Bacterial DNA Damage or Repair Tests
798.5500
84-2
none
712-C-98-229
Aug-98
870.5550
Unscheduled DNA Synthesis in Mammalian Cells in Culture
798.5550
84-2
482
712-C-98-230
Aug-98
870.5575
Mitotic Gene Conversion in Saccharomyces cerevisiae
798.5575
84-2
481
712-C-98-232
Aug-98
870.5900
In Vitro Sister Chromatid Exchange Assay
798.5900
84-2
479
712-C-98-234
Aug-98
870.5915
In Vitro Sister Chromatid Exchange Assay
798.5195
84-2
none
712-C-98-235
Aug-98







Group E—Neurotoxicity Test Guidelines.
870.6100
Acute and 28-day Delayed Neurotoxicity of Organophosphorus
Substances
798.6450,
798.6540,
798.6560
81-7,
82-5,
82-6
418, 419
712-C-98-237
Aug-98
870.6200
Neurotoxicity Screening Battery
798.6050,
798.6200,
798.6400
81-8,
82-7,
83-1
424
712-C-98-238
Aug-98
870.6300
Developmental Neurotoxicity Study
none
83-6
none
712-C-98-239
Aug-98
870.6500
Schedule-Controlled Operant Behavior
798.6500
85-5
none
712-C-98-240
Aug-98
870.6850
Peripheral Nerve Function
798.6850
85-6
none
712-C-98-241
Aug-98
870.6855
Neurophysiology: Sensory Evoked Potentials
798.6855
none
none
712-C-98-242
Aug-98







Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 870—Health Effects Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0156 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Group F—Special Studies Test Guidelines.
870.7200
Companion Animal Safety
none
none
none
712-C-98-349
Aug-98
870.7485
Metabolism and Pharmacokinetics
798.7485
85-1
417
712-C-95-244
Aug-98
870.7600
Dermal Penetration
none
85-3
none
712-C-98-350
Aug-98
870.7800
Immunotoxicity
none
85-7
none
712-C-98-351
Aug-98







Group G—Health Effects Chemical-Specific Test Guidelines
870.8223
[Reserved]





870.8245
[Reserved]





870.8300
[Reserved]





870.8320
[Reserved]





870.8340
[Reserved]





870.8355
Combined Chronic Toxicity/Carcinogenicity Testing of Respirable
Fibrous Particles
798.3320
none
none
712-C-01-352
Jul-01
870.8360
[Reserved]





870.8380
[Reserved]





870.8500
[Reserved]





870.8600
[Reserved]





870.8700
[Reserved]





870.8800
[Reserved]












DRAFT 870 Test Guidelines
Please note, those guidelines labeled as "Public Draft" are are not yet available in final form. Although you may consult these guidelines, please check
with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA or TSCA.
870.1350
Acute Inhalation Toxicity with Histopathology
none
none
none
712-C-96-291
Jun-96
870.3500
Preliminary Developmental Toxicity Screen
none
none
none
712-C-96-205
Jun-96
870.3600
Inhalation Developmental Toxicity Study
none
none
none
712-C-96-206
Jun-96
870.5265
The Salmonella typhimurium Reverse Mutation Assay
none
none
none
712-C-96-219
Jun-96
870.8223
Pharmacokinetic Test
none
none
none
712-C-96-250
Jun-96
870.8245
Dermal Pharmacokinetics ofDGBE and DGBA
none
none
none
712-C-96-251
Jun-96
870.8300
Dermal Absorption for Compounds that are Volatile and Metabolized
to Carbon Dioxide
none
none
none
712-C-96-252
Jun-96
870.8320
Oral/Dermal Pharmacokinetics
none
none
none
712-C-96-253
Jun-96
Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 870—Health Effects Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0156 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT
OPP
OECD
870.8340
Oral and Inhalation Pharmacokinetic Test
none
none
none
712-C-96-254
Jun-96
870.8360
Pharmacokinetics of Isopropanal
none
none
none
712-C-96-255
Jun-96
870.8380
Inhalation and Dermal Pharmacokinetics of Commercial Hexane
none
none
none
712-C-96-256
Jun-96
870.8500
Toxicokinetic Test
none
none
none
712-C-96-257
Jun-96
870.8600
Developmental Neurotoxicity Screen
none
none
none
712-C-96-258
Jun-96
870.8700
Subchronic Oral Toxicity Test
none
none
none
712-C-96-259
Jun-96
870.8800
Morphologic Transformation of Cells in Culture
none
none
none
712-C-96-260
Jun-96







Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 875—Occupational and Residential Exposure Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0157 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Final 875 Test Guidelines
Group A—Applicator Exposure Monitoring Test Guidelines.
875.1000
Background for Application Exposure Monitoring Test Guidelines
none
230
none
712-C-96-261
Feb-96
875.1100
Dermal Exposure—Outdoor
none
231
none
712-C-96-262
Feb-96
875.1200
Dermal Exposure—Indoor
none
233
none
712-C-96-209
Feb-96
875.1300
Inhalation Exposure—Outdoor
none
232
none
712-C-96-263
Feb-96
875.1400
Inhalation Exposure—Indoor
none
234
none
712-C-96-213
Feb-96
875.1500
Biological Monitoring
none
235
none
712-C-96-264
Feb-96
875.1600
Application Exposure Monitoring Data Reporting
none
236
none
712-C-96-265
Feb-96







Group B—Postapplication Exposure Monitoring Test Guidelines.
875.2000
Background for Postapplication Exposure Monitoring Test Guidelines
none
130, 131
none
712-C-96-266
Feb-96
875.2100
Foliar Dislodgeable Residue Dissipation
none
132-1
none
712-C-96-267
Feb-96
875.2200
Soil Residue Dissipation
none
132-1
none
712-C-96-243
Feb-96
875.2400
Dermal Exposure
none
133-3
none
712-C-96-269
Feb-96
875.2500
Inhalation Exposure
none
133-4
none
712-C-96-270
Feb-96
875.2600
Biological Monitoring
none
235
none
712-C-96-271
Feb-96
875.2800
Descriptions of Human Activity
none
133-1
none
712-C-96-283
Feb-96
875.2900
Data Reporting and Calculations
none
134
none
712-C-96-272
Feb-96







DRAFT 875 Test Guidelines
Please note, those guidelines labeled as "Public Draft" are are not yet available in final form. Although you may consult these guidelines, please check
with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA or TSCA.

None.












Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 880—Biochemicals Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0158 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Final 880 Test Guidelines
Group A—Product Analysis Test Guidelines.
880.1100
Product Identity and Composition
none
151-10
none
712-C-96-273
Feb-96
880.1200
Description of Starting Materials, Production and Formulation
Process
none
151-11
none
712-C-96-274
Feb-96
880.1400
Discussion of Formation of Impurities
none
151-12
none
712-C-96-275
Feb-96







Group B—Toxicology Test Guidelines.
880.3550
Immunotoxicity
none
152-18
none
712-C-96-280
Feb-96
880.3800
Immune Response
none
152-24
none
712-C-96-281
Feb-96







Group C—Nontarget Organisms and Environmental Testing Test Guidelines.
880.4350
Nontarget Insect Testing
none
154-11
none
712-C-96-285
Feb-96
880.4425
Dispenser Water Leaching
none
155-5
none
712-C-96-286
Feb-96







DRAFT 880 Test Guidelines
Please note, those guidelines labeled as "Public Draft" are are not yet available in final form. Although you may consult these guidelines, please check
with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA or TSCA.

None.












Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 885—Microbial Pesticide Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0159 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Final 885 Test Guidelines
885.0001
Overview for Microbial Pest Control Agents
none
150A
none
712-C-96-280
Feb-96







Group A—Product Analysis Test Guidelines.
885.1100
Product Identity
none
151A-10
none
712-C-96-273
Feb-96
885.1200
Manufacturing Process
none
151A-11
none
712-C-96-293
Feb-96
885.1300
Discussion of Formation of Unintentional Ingredients
none
151A-01
none
712-C-96-294
Feb-96
885.1400
Analysis of Samples
none
151A-13
none
712-C-96-295
Feb-96
885.1500
Certification of Limits
none
151A-15
none
712-C-96-296
Feb-96







Group B—Residues Test Guidelines.
885.2000
Background for Residue Analysis of Microbial Pest Control Agents
none
153A-1
none
712-C-96-299
Feb-96
885.2100
Chemical Identity
none
153A-4
none
712-C-96-300
Feb-96
885.2200
Nature of the Residue in Plants
none
153A-6
none
712-C-96-302
Feb-96
885.2250
Nature of the Residue in Animals
none
153A-7
none
712-C-96-303
Feb-96
885.2300
Analytical Methods—Plants
none
153A-8a
none
712-C-96-304
Feb-96
885.2350
Analytical Methods—Animals
none
153A-8b
none
712-C-96-305
Feb-96
Document incorrectly says publication Number ends in "-304".
885.2400
Storage Stability
none
153A-9
none
712-C-96-306
Feb-96
885.2500
Magnitude of Residues in Plants
none
153A-10
none
712-C-96-307
Feb-96
885.2550
Magnitude of Residues in Meat, Milk, Poultry, Eggs
none
153A-11
none
712-C-96-308
Feb-96
885.2600
Magnitude of Residues in Potable Water, Fish, and Irrigated Crops
none
153A-01
none
712-C-96-309
Feb-96







Group C—Toxicology Test Guidelines.
885.3000
Background-Mammalian Toxicity/Pathogenicity/lnfectivity
none
152A-1
none
712-C-96-314
Feb-96
885.3050
Acute Oral Toxicity/Pathogenicity
none
152 A-10
none
712-C-96-315
Feb-96
885.3100
Acute Dermal Toxicity/Pathology
none
152 A-11
none
712-C-96-316
Feb-96
885.3150
Acute Pulmonary Toxicity/Pathogenicity
none
152A-12
none
712-C-96-317
Feb-96
Document incorrectly says publication Number ends in "-318".
885.3200
Acute Injection Toxicity/Pathogenicity
none
152A-13
none
712-C-96-318
Feb-96
885.3400
Hypersensitivity Incidents
none
152A-15
none
712-C-96-320
Feb-96
885.3500
Cell Culture
none
152A-16
none
712-C-96-321
Feb-96
885.3550
Acute Toxicology, Tier II
none
152A-20
none
712-C-96-322
Feb-96
Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 885—Microbial Pesticide Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0159 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT
OPP
OECD
885.3600
Subchronic Toxicity/Pathogenicity
none
152A-21
none
712-C-96-323
Feb-96

Document incorrectly says publication Number ends in "-232".
885.3650
Reproductive/Fertility Effects
none
152A-30
none
712-C-96-324
Feb-96







Group D—Nontarget Organism and Environmental Expression Test Guidelines
885.4000
Background for Nontarget Organism Testing of Microbial Pest
Control Agents
none
154A-1,
154A-2,
154A-3,
154A-4,
154A-5
none
712-C-96-328
Feb-96
885.4050
Avian Oral, Tier I
none
154A-16
none
712-C-96-329
Feb-96
885.4100
Avian Inhalation Test, Tier I
none
154A-17
none
712-C-96-330
Feb-96
885.4150
Wild Mammal Testing, Tier I
none
154A-18
none
712-C-96-331
Feb-96
885.4200
Freshwater Fish Testing, Tier I
none
154A-19
none
712-C-96-332
Feb-96
885.4240
Freshwater Aquatic Invertebrate Testing, Tier I
none
154A-20
none
712-C-96-333
Feb-96
885.4280
Estuarine and Marine Animal Testing, Tier I
none
154A-21
none
712-C-96-334
Feb-96
885.4300
Nontarget Plant Studies, Tier I
none
154A-22
none
712-C-96-335
Feb-96
885.4340
Nontarget Insect Testing, Tier I
none
154A-23
none
712-C-96-336
Feb-96
885.4380
Honey Bee Testing, Tier I
none
154A-24
none
712-C-96-337
Feb-96
885.4600
Avian Chronic Pathogenicity and Reproduction Test, Tier III
none
154A-26
none
712-C-96-342
Feb-96
885.4650
Aquatic Invertebrate Range Testing, Tier III
none
154A-27
none
712-C-96-343
Feb-96
885.4700
Fish Life Cycle Studies, Tier III
none
154A-28
none
712-C-96-344
Feb-96
885.4750
Aquatic Ecosystem Test
none
154A-29
none
712-C-96-345
Feb-96







Group E—Environmental Expression Test Guidelines.
885.5000
Background for Microbial Pesticides Testing
none
155 A-1,2
none
712-C-96-056
Feb-96
885.5200
Expression in a Terrestrial Environment
none
155A-10
none
712-C-96-338
Feb-96
885.5300
Expression in a Freshwater Environment
none
155A-11
none
712-C-96-339
Feb-96
885.5400
Expression in a Marine or Estuarine Environment
none
155A-12
none
712-C-96-312
Feb-96







DRAFT 885 Test Guidelines
Please note, those guidelines labeled as "Public Draft" are are not yet available in final form. Although you may consult these guidelines, please check
with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA or TSCA.
Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 885—Microbial Pesticide Test Guidelines
(Docket ID No. EPA-HQ-QPPT-2009-0159 @ http://www.requlations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT
OPP
OECD

None.












Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 890—Endocrine Distruptor Screening Program Test Guidelines
(Docket ID No. EPA-HQ-OPPT-2009-	@ http://www.regulations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT OPP OECD
Final 890 Test Guidelines
890.0001
[Reserved]
none
none
none









Group A—EC
JSP Tier 1 Test Guidelines.
890.1100
Amphibian Metamorphosis (Frog)
none
none
231

Jul-09
890.1150
Androgen Receptor Binding (Rat Prostate Cytosol)
none
none


Jul-09
890.1200
Aromatase (Human Recombinant)
none
none


Jul-09
890.1250
Estrogen Receptor Binding (Rat Uterine Cytosol)
none
none


Jul-09
890.1300
Estrogen Receptor Transcriptional Activation (Human Cell Line
(HeLa-9903))
none
none
455

Jul-09
890.1350
Fish Short-term Reproduction
none
none
299

Jul-09
890.1400
Hershberger (Rat)
none
none
441

Jul-09
890.1450
Female Pubertal (Rat)
none
none


Jul-09
890.1500
Male Pubertal (Rat)
none
none


Jul-09
890.1550
Steroidogenesis (Human Cell Line - H295R)
none
none


Jul-09
890.1600
Uterotrophic (Rat)
none
none
440

Jul-09







Group B—EC
JSP Tier 2 Test Guidelines.
890.2100
[Reserved]
none
none



890.2150
[Reserved]
none
none



890.2200
[Reserved]
none
none



890.2250
[Reserved]
none
none



890.2300
[Reserved]
none
none










DRAFT 890 Test Guidelines
Please note, those guidelines labeled as "Public Draft" are are not yet available in final form. Although you may consult these guidelines, please check
with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA or TSCA.
The following Tier 2 assays are being developed for EDSP and information on the assay or their status under the EDSP validation process is posted at
http:/Avww. epa. gov/scipoly/oscpendo/index. htm.

Amphibian Development, Reproduction






Avian 2-Generation






Fish Lifecycle






Invertebrate Lifecycle






Mammalian 2-Generation





Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
Series 890—Endocrine Distruptor Screening Program Test Guidelines
(Docket ID No. EPA-HQ-OPPT-2009-	@ http://www.regulations.gov)
	As of May 2009	
OPPTS
Guideline No.
Guideline Names
Other Reference Numbers
EPA Pub No.
Date
Issued
OPPT
OPP
OECD

In Utero through Lactation












Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
OPPTS Harmonized Test Guidelines - Public DRAFTS (Not Yet Final)
As of May 2009
This is a compilation of the guidelines that have been issued in draft, but have not yet been issued in final form. Although you may consult these guidelines,
please check with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA orTSCA.
Guideline
No.
Guideline Name
Other Reference Numbers
EPA Pub
No.
Date
Issued
Docket ID No.
Comment
Period
OPPT
OPP
OECD
810.3700
Insect Repellents for Human Skin and
Outdoor Premises
none
95-9,
95-10
none
712-C-99-369
Dec-99
n/a
Closed
850.1000
Special Consideration for Conducting
Aquatic Laboratory Studies
none
none
none
712-C-96-113
Apr-96
n/a
Closed
850.1010
Aquatic Invetebrate Acute toxicity, Test,
Freshhwater Daphnids
797.1300
72-2
none
712-C-96-114
Apr-96
n/a
Closed
850.1020
Gammarid Acute Toxicity Test
795.120
none
none
712-C-96-130
Apr-96
n/a
Closed
850.1025
Oyster Acute Toxicity Test (Shell
Deposition)
797.1800
72-3
none
712-C-96-115
Apr-96
n/a
Closed
850.1035
Mysid Acute Toxicity Test
797.1930
72-3
none
712-C-96-136
Apr-96
n/a
Closed
850.1045
Penaeid Acute Toxicity Test
797.1970
72-3
none
712-C-96-137
Apr-96
n/a
Closed
850.1055
Bivalve Acute Toxicity Test (Embryo Larval)
none
72-3
none
712-C-96-100
Apr-96
n/a
Closed
850.1075
Fish Acute Toxicity Test, Freshwater and
Marine
797.1400
72-1,
72-3
203
712-C-96-118
Apr-96
n/a
Closed
850.1085
Fish Acute Toxicity Mitigated by Humic Acid
797.1460
none
none
712-C-96-117
Apr-96
n/a
Closed
850.1300
Daphnid Chronic Toxicity Test
797.1330
72-4
202
712-C-96-120
Apr-96
n/a
Closed
850.1350
Mysid Chronic Toxicity Test
797.1950
72-4
none
712-C-96-166
Apr-96
n/a
Closed
Document incorrectly says publication Number ends in "-120".
850.1400
Fish Early-Life Stage Toxicity Test
797.1000
72-4
210
712-C-96-121
Apr-96
n/a
Closed
850.1500
Fish Life Cycle Toxicity
none
72-5
none
712-C-96-122
Apr-96
n/a
Closed
850.1710
Oyster BCF
797.1830
72-6
none
712-C-96-127
Apr-96
n/a
Closed
850.1730
Fish BCF
797.1520
72-6,
165-4
305
712-C-96-129
Apr-96
n/a
Closed
850.1735
Whole Sediment Acute Toxicity
Invertebrates, Freshwater
none
none
none
712-C-96-354
Apr-96
n/a
Closed
850.1740
Whole Sediment Acute Toxicity
Invertebrates, Marine
none
none
none
712-C-96-355
Apr-96
n/a
Closed
850.1790
Chironomid Sediment Toxicity Test
795.135
none
none
712-C-96-313
Apr-96
n/a
Closed
Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
OPPTS Harmonized Test Guidelines - Public DRAFTS (Not Yet Final)
As of May 2009
This is a compilation of the guidelines that have been issued in draft, but have not yet been issued in final form. Although you may consult these guidelines,
please check with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA orTSCA.
Guideline
No.
Guideline Name
Other Reference Numbers
EPA Pub
No.
Date
Issued
Docket ID No.
Comment
Period
OPPT
OPP
OECD
850.1800
Tadpole/Sediment Subchronic Toxicity Test
797.1995
none
none
712-C-96-132
Apr-96
n/a
Closed
850.1850
Aquatic Food Chain Transfer
none
72-6
none
712-C-96-133
Apr-96
n/a
Closed
850.1900
Generic Freshwater Microcosm Test,
Laboratory
797.3050,
797.3100
none
none
712-C-96-134
Apr-96
n/a
Closed
850.1925
Site-Specific Aquatic Microcosm Test,
Laboratory
797.3100
none
none
712-C-96-173
Apr-96
n/a
Closed
850.1950
Field Testing for Aquatic Organisms
none
72-7,
165-5
none
72-C-96-135
Apr-96
n/a
Closed
850.2100
Avian Acute Oral Toxicity Test
797.2175
71-1
none
712-C-96-139
Apr-96
n/a
Closed
850.2200
Avian Dietary Toxicity Test
797.2050
71-2
205
712-C-96-140
Apr-96
n/a
Closed
850.2300
Avian Reproduction Test
797.2130,
797.2150
71-4
206
712-C-96-141
Apr-96
n/a
Closed
850.2400
Wild Mammal Acute Toxicity
none
71-3
none
712-C-96-142
Apr-96
n/a
Closed
850.2450
Terrestrial (Soil-Core) Microcosm Test
797.3775
none
none
712-C-96-143
Apr-96
n/a
Closed
850.2500
Field Testing for Terrestrial Wildlife
none
71-5
none
712-C-96-144
Apr-96
n/a
Closed
850.3020
Honey Bee Acute Contact Toxicity
none
141-1
none
712-C-96-147
Apr-96
n/a
Closed
850.3030
Honey Bee Toxicity of Residues on Foliage
none
141-2
none
712-C-96-148
Apr-96
n/a
Closed
850.3040
Field Testing for Pollinators
none
141-5
none
712-C-96-150
Apr-96
n/a
Closed
850.4000
Background—Nontarget Plant Testing
none
120-1
none
712-C-96-151
Apr-96
n/a
Closed
850.4025
Target Area Phytotoxicity
none
121-1
none
712-C-96-152
Apr-96
n/a
Closed
850.4100
Terrestrial Plant Toxicity, Tier 1 (Seedling
Emergence)
none
122-1
none
712-C-96-153
Apr-96
n/a
Closed
850.4150
Terrestrial Plant Toxicity, Tier 1 (Vegetative
Vigor)
none
122-1
none
712-C-96-163
Apr-96
n/a
Closed
850.4200
Seed Germination/Root Elongation Toxicity
Test
797.2750
122-1
none
712-C-96-154
Apr-96
n/a
Closed
850.4225
Seedling Emergence, Tier II
797.2750
123-1
none
712-C-96-313
Apr-96
n/a
Closed
850.4230
Early Seedling Growth Toxicity Test
797.2800
123-1
none
712-C-96-347
Apr-96
n/a
Closed
Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
OPPTS Harmonized Test Guidelines - Public DRAFTS (Not Yet Final)
As of May 2009
This is a compilation of the guidelines that have been issued in draft, but have not yet been issued in final form. Although you may consult these guidelines,
please check with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA orTSCA.
Guideline
No.
Guideline Name
Other Reference Numbers
EPA Pub
No.
Date
Issued
Docket ID No.
Comment
Period
OPPT
OPP
OECD
850.4250
Vegetative Vigor, Tier II
797.2750
123-1
none
712-C-96-364
Apr-96
n/a
Closed
850.4300
Terrestrial Plants Field Study, Tier III
none
124-1
none
712-C-96-155
Apr-96
n/a
Closed
850.4400
Aquatic Plant Toxicity Test using Lemna
spp. Tiers 1 and II
797.1160
122-2,
123-2
none
712-C-96-156
Apr-96
n/a
Closed
850.4450
Aquatic Plants Field Study, Tier III
none
124-2
none
712-C-96-157
Apr-96
n/a
Closed
850.4600
Rhizobium-Legume Toxicity
797.2900
none
none
712-C-96-158
Apr-96
n/a
Closed
850.4800
Plant Uptake and Translocation Test
797.2850
none
none
712-C-96-159
Apr-96
n/a
Closed
850.5100
Soil Microbial Community Toxicity Test
797.3700
none
none
712-C-96-161
Apr-96
n/a
Closed
850.5400
Algal Toxicity, Tiers 1 and II
797.1050
122-2,
123-2
none
712-C-96-164
Apr-96
n/a
Closed
850.6200
Earthworm Subchronic Toxicity Test
795.150
none
207
712-C-96-167
Apr-96
n/a
Closed
850.6800
Modified Activated Sludge, Respiration
Inhibition Test for Sparingly Soluble
Chemicals
795.170
none
209
712-C-96-168
Apr-96
n/a
Closed
850.7100
Data Reporting for Environmental
Chemistry Methods
none
none
none
712-C-96-348
Apr-96
n/a
Closed
870.1350
Acute Inhalation Toxicity with
Histopathology
none
none
none
712-C-96-291
Jun-96
n/a
Closed
870.3500
Preliminary Developmental Toxicity Screen
none
none
none
712-C-96-205
Jun-96
n/a
Closed
870.3600
Inhalation Developmental Toxicity Study
none
none
none
712-C-96-206
Jun-96
n/a
Closed
870.5265
The salmonella typhimurium Reverse
Mutation Assay
none
none
none
712-C-96-219
Jun-96
n/a
Closed
870.8223
Pharmacokinetic Test
none
none
none
712-C-96-250
Jun-96
n/a
Closed
870.8245
Dermal Pharmacokinetics ofDGBE and
DGBA
none
none
none
712-C-96-251
Jun-96
n/a
Closed
870.8300
Dermal Absorption for Compounds that are
Volatile and Metabolized to Carbon Dioxide
none
none
none
712-C-96-252
Jun-96
n/a
Closed
870.8320
Oral/Dermal Pharmacokinetics
none
none
none
712-C-96-253
Jun-96
n/a
Closed
Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------
OPPTS Harmonized Test Guideline - Series Index
OPPTS Harmonized Test Guidelines - Public DRAFTS (Not Yet Final)
As of May 2009
This is a compilation of the guidelines that have been issued in draft, but have not yet been issued in final form. Although you may consult these guidelines,
please check with the appropriate office before you use a draft guideline to generate data for submission to EPA under FIFRA, FFDCA orTSCA.
Guideline
No.
Guideline Name
Other Reference Numbers
EPA Pub
No.
Date
Issued
Docket ID No.
Comment
Period
OPPT
OPP
OECD
870.8340
Oral and Inhalation Pharmacokinetic Test
none
none
none
712-C-96-254
Jun-96
n/a
Closed
870.8360
Pharmacokinetics of Isopropanal
none
none
none
712-C-96-255
Jun-96
n/a
Closed
870.8380
Inhalation and Dermal Pharmacokinetics of
Commercial Hexane
none
none
none
712-C-96-256
Jun-96
n/a
Closed
870.8500
Toxicokinetic Test
none
none
none
712-C-96-257
Jun-96
n/a
Closed
870.8600
Developmental Neurotoxicity Screen
none
none
none
712-C-96-258
Jun-96
n/a
Closed
870.8700
Subchronic Oral Toxicity Test
none
none
none
712-C-96-259
Jun-96
n/a
Closed
870.8800
Morphologic Transformation of Cells in
Culture
none
none
none
712-C-96-260
Jun-96
n/a
Closed









Available electronically at http://www.epa.gov/opptsfrs/home/testmeth.htm.

-------