E P A/600/R-20/087
ECOTOX
ECOTOXicology Knowledgebase System
User Guide - Version 5.3
Prepared by: Colleen M. Elonen
U.S. Environmental Protection Agency (EPA)
Office of Research and Development (ORD)
Center for Computational Toxicology and Exposure (CCTE)
Great Lakes Toxicology and Ecology Division (GLTED) Duluth, Minnesota
In Conjunction with:
General Dynamics Information Technology (GDIT), Duluth, Minnesota
Contract CIO-SP3, HHSN316201200013W
Task Order: EP-G16H-01256, SMAVCS3
TDD 2-8 ECOTOX Application Development and Support
March 2020
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ECOTOX 5.3 User Guide
DISCLAIMER
Users should consult the original scientific paper to ensure an understanding of
the context of the data retrieved from the ECOTOX Knowledgebase.
ECOTOX attempts to be comprehensive, but the searches may not locate all
literature relevant to a research program. In addition, the time lag from conducting a
literature search, acquiring the publication and extracting the data into the ECOTOX
Knowledgebase can be up to, or exceed, six months. For this reason, it is suggested
that users conduct searches of the most recent publication year to ensure data may
be captured which has not been entered in to the ECOTOX Knowledgebase or met
the criteria for inclusion.
Reference herein to any specific commercial product, process, or service by trade
name, trademark, manufacturer, or otherwise, does not necessarily constitute or imply
its endorsement, recommendation, or favoring by the U. S. government.
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Table of Contents
INTRODUCTION 1
GETTING STARTED 2
Access 2
Navigating within ECOTOX 2
Home 2
Search 4
Explore 5
OVERVIEW OF SEARCH OPTIONS 5
Search Strategy Basics 5
Overview of Search and Explore Pages 5
Moving Within Pages and Target Menu 5
Selection Box and Types 6
Key Functions 6
Options for Searching in ECOTOX 6
ECOTOX DATA SEARCHES 10
Chemical Entry 10
Predefined Chemical Groups 11
Search Tips for Chemicals 11
Search by Effect Measurements 11
Search by Endpoints 12
Taxonomic Entry 13
Predefined Species Groups 14
Search Tips for Taxonomic Searches 14
Test Location 15
Exposure Media 15
Exposure Type 15
Control Type 16
Chemical Analysis 16
Author(s) 17
Reference Number 17
Independently Compiled Data 17
Recent Modifications and Additions 17
Delimited Output 18
Refining/Filtering data using Query filters 20
Data Visualization Plots 20
Refining/Filtering data using Query filters 22
Data Visualization Plots 22
Refining/Filtering data using Query filters 23
Data Visualization Plots 24
EXITING ECOTOX 24
APPENDIX A: ECOTOX SEARCH PLANNING FORM 25
Chemicals 25
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Species 25
Test Results 26
Test Conditions 26
Publications 27
Report Output 28
APPENDIX B: PRACTICE SEARCHES 30
APPENDIX C: ECOTOX KNOWLEDGEBASE OVERVIEW 35
Data Sources 35
Quality Assurance 36
Aquatic Data Elements 36
Aquatic Effect Parameters 36
Terrestrial Data Elements 37
Test Identification 38
References 38
APPENDIX D: SPECIES AND CHEMICAL VERIFICATION 39
Species Verification 39
Taxonomic Hierarchy 39
Chemical Verification 41
APPENDIX E: ECOTOX DATA FIELD DESCRIPTIONS 42
Chemical Fields 42
Species Fields 42
Test Condition Fields 43
Application Frequency 43
Media Type 43
Test Location 43
Exposure Duration 43
Study Duration 44
Exposure Type 44
Habitat 44
Chemical Analysis 44
Application Frequency 44
Study Type 45
Test Type 45
Test Method 45
Control 45
Number of Doses 45
Doses 45
Experimental Design 45
Gender 45
Ionic Fraction 46
Dose Statistical Method 46
Test Result Parameters 46
Endpoint 46
Effect 46
Trend 47
Response Site 47
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EE_Comment 47
Effect % 48
Statistical Level 48
Bioconcentration 48
Concentration Type 48
Endpoint Assignment 48
Concentration/Dose 48
Result Sample Number 49
Observed Duration 49
Observed Response (Dose Response only) 49
Result Statistical Method (Dose Response only) 49
Result % Dry/Wet Weight () 49
Result Percent Lipid () 49
Other Effects 50
General Comment 50
Water Chemistry Fields (Aquatic only) 50
Outdoor Test Fields 50
Habitat Code 50
Habitat Comment 50
Substrate Code and Comment 50
Geographic Code 50
Geographic Location 50
Application Rate 51
Application Date/Season 51
Terrestrial Data Elements 52
Test Record 52
Test Number 52
Exposure Number (Delimited format only) 52
Parent Record and Result Number (Delimited format only) 52
Test Comment 52
Exposure Comment 52
Result Comment 53
Soil Parameter Fields (Terrestrial only) 53
Soil Type 53
Soil Sand %, Soil Silt %, Soil Clay % 53
Soil pH 53
Media Organic Matter 53
Media Moisture 53
Media Cation Exchange Capacity 53
Soil Dose Measured 53
Media Measurement (wet/dry) 54
APPENDIX F: INDEPENDENTLY COMPILED DATA FILES 54
APPENDIX G: COMMENT FIELD HEADER NAMES 58
Aquatic Comment Abbreviations 58
Terrestrial Comment Abbreviations 60
APPENDIX H: DELIMITED OUTPUT HEADER NAMES 63
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Aquatic Report Output Codes 63
Terrestrial Delimited Report Output Codes 75
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INTRODUCTION
In the development of and implementation for ecosystem management decisions
there is the need to establish scientifically credible risk assessments for chemical
stressors. Ecological assessments are required to characterize and diagnose the
relative risk of chemical pollutants and to predict future risk as a function of
environmental management options.
The U.S. EPA's ECOTOXicology Knowledgebase (ECOTOX) is a source for locating
single chemical toxicity data for aquatic life, terrestrial plants and wildlife. ECOTOX was
created and is maintained by the Office of Research and Development's (ORD's) Duluth
MN laboratory.
ECOTOX integrates toxicity data derived predominantly from the peer-reviewed
literature, for aquatic life, terrestrial plants, and terrestrial wildlife. Not all data published
in the peer review ecotoxicology literature are included in ECOTOX. Users are
encouraged to refer to the Limitations section of this document to understand test
results that are not considered for inclusion in the database.
Researchers or managers using ECOTOX for analyses or summary projects
should consult the original scientific paper to ensure an understanding of the
context of the data retrieved from ECOTOX.
For more information on the ECOTOX Knowledgebase contact:
ECOTOX Support
Office of Research and Development (ORD)
Center for Computational Toxicology and Exposure (CCTE)
Great Lakes Toxicology and Ecology Division (GLTED)
6201 Congdon Boulevard
Duluth, Minnesota 55804
Telephone: 218-529-5225
Fax: 218-529-5003
E-mail: ecotox.support@epa.gov
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ECOTOX 5.3 User Guide
GETTING STARTED
Access
To access the ECOTOX website, users need a computer equipped with a JavaScript
enabled browser and internet connectivity. Start browser software and type in the
Internet address http://www.epa.gov/ecotox/ to be connected to the ECOTOX Home
page.
ECOTOX has the following browser limitations:
• The query pages require that the browser supports JavaScript and this feature must
be activated in browser preferences.
• ECOTOX has been tested using FireFox, Internet Explorer and Google Chrome. Chrome or
Firefox are currently the preferred browser programs for optimal performance.
• If a popup blocker program is activated, ECOTOX Reports, Help and Browse
features will not display. Please add the ECOTOX website to popup browser
exception list to ensure full usability.
Navigating within ECOTOX
Home
The Home page provides a general overview of the ECOTOX Knowledgebase with links
to many of the application pages and links to more information for statistics and other
databases. These selections will open the requested information. To avoid losing your
current Explore and Search selections, left click the link and open tab in a new window.
About ECOTOX: This section of the website provides a general overview of the
ECOTOX Knowledgebase, including the history of the system's development, and
Help Center linkages which describes the ECOTOX website contents and navigational
resources available.
This printable User Guide is available in PDF (Portable Document Format) under the
PDF Documentation section as "ECOTOX User Guide" and the "ECOTOX Term
Appendix".
The Help center resources are listed in one of four web pages:
Starting Out - Information on how to begin navigating and searching the ECOTOX
website. This page also provides links to the limitations associated with the
ECOTOX Knowledgebase, Recent additions, Navigational help, Frequent questions
and PDF versions of the ECOTOX User Guide (this document), and the ECOTOX
Term Appendix list, which provides detailed information regarding terms used in the
aquatic and terrestrial reports.
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How do I... - Provides information on how to perform searches, select Search
parameters and Report format, and Navigate or retrieve outputs in ECOTOX.
What is... - Provides links to Data Field definitions and terms for each field available
within ECOTOX searches and output. It also provides a Database overview on data
sources, including Species and Chemical verification sources used by ECOTOX
staff. For a brief overview of data extraction processes used within ECOTOX see
Appendix C.
More Resources - Includes a glossary of terms and links to related websites.
Providing these links does not imply endorsement by the U.S. EPA.
Note: ECOTOX Limitations: The following restrictions are placed on ECOTOX data.
Data not satisfying these requirements are excluded from the ECOTOX
Knowledgebase. Users should review the limitations of ECOTOX data retrieval and
system requirements prior to performing searches on this site.
Criteria
Requirement/Inclusions
Limitations/Exclusions
Chemical
Single chemicals relevant to
environmental exposure
Verifiable Chemical Abstract
Services (CAS) number
Mixtures (petroleum fuels)
Air pollution (CO2 and ozone)
Species
Ecologically relevant
species
Priority species are wild
(test results for terrestrial
domestic and laboratory
species are used to fill data
gaps when needed)
Organism taxonomic
information verifiable
against standard taxonomic
sources
Human, monkey, bacteria, viral
and yeast
Effect/
Response
Biological effect on live,
whole organisms
Adverse effects are priority
(beneficial or nutritional
effects are lower priority)
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Criteria
Requirement/Inclusions
Limitations/Exclusions
Concentration/
Dose
Concurrent environmental
chemical concentration or
dose reported as
concentration, dose or
application rate.
Sediment studies which
have a reported water
concentration
Inhalation dose route (including
intratracheal instillation)
Lead shot
Sediment only concentration
Log values
Exposure
Duration
Duration reports an
associated exposure
concurrent with a
biological effect
Publication /Data
Format
Primary data source
Full text English (some Non-
-English papers have
an English abstract
with data extracted)
Reviews
Full text foreign language
Abstract only format
ECOTOX system limitations:
• In 'Explore' there is a maximum of 3,000 Records that can be viewed but
unlimited data records can be retrieved.
• If popup blocker program is used, ECOTOX report exports may not display. Please
add the ECOTOX website to popup browser exception list to ensure full usability.
Data Downloads: Users can download delimited ASCII files of the entire aquatic or
terrestrial raw data. This does not include any software and will require combining
various files together to view entire data records. The data are divided into two sections;
Aquatic and Terrestrial. Within these sections you will find data tables, field descriptions
and graphical relations of the data structure.
Search
The ECOTOX Knowledgebase 'Search' function provides a direct method to retrieve
data that can be refined by limiting the search parameters, e.g. Chemical, Effects,
Endpoints or Species. Users can refine or filter selections before continuing a search.
Once search options have been selected or updated, users can view the report in the
browser, change the data fields, or export the report in an Excel or delimited format.
'Search' utilizes all available search and output features.
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Explore
The ECOTOX Knowledgebase 'Explore' function is an interactive way to examine
search paths by Chemical, Species, or Effects via Groups. Once a Group is selected to
explore, query filters will be displayed to refine data via drop down boxes or output
table.
During the exploration there are also options to examine the data visually via plotting
functions. Once data exploration is complete, users are able to select report output
options or forward 'Explore' parameters to the ECOTOX 'Search' for further
refinement.
OVERVIEW OF SEARCH OPTIONS
Database retrievals can be conducted using either 'Search' or 'Explore'. 'Search'
supports queries on species, chemicals, effects, endpoints, test conditions or by
publication. 'Search' includes all options under 'Explore' and enables you to focus on
additional criteria such as study site type (e.g., laboratory, field), exposure media (e.g.,
freshwater, soil), route of chemical exposure (e.g., oral, diet), and statistically-derived
endpoints (e.g., LD50, NOEL). 'Search' results can be downloaded either as a
Microsoft (MS) Excel spreadsheet or an ASCII delimited file format, which can be
transferred into a database or spreadsheet.
When you are within the 'Search' page and you click on inside of a text entry box or
output display field, context-sensitive help will display in a separate window. You may
navigate within the help window without affecting your search session.
Search Strategy Basics
The 'Search' or 'Explore' pages are each designed to search on all data, unless you
restrict the search by choosing specific search criteria (e.g., adding the check in the
checkbox, enter text in a text entry field). You may perform the search at any time after
you have specified your search criteria. You do not need to enter something in every
'Search' criteria area.
The search logic includes two basic strategies: combination/union and intersection.
Within a Search area (e.g., chemical), the search will combine all your search
selections. Between each search area, the search will intersect your selections (e.g.,
intersection between chemical and taxonomic selections). Users may want to use the
ECOTOX Search Planner located in Appendix A to plan searches. Appendix B
describes practice searches to assist users in using the ECOTOX system.
Before searching, users should read Appendix C to find out more about the
ECOTOX Knowledgebase and Appendix E for specific data field descriptions.
Overview of Search and Explore Pages
Moving Within Pages and Target Menu
Users may move within an ECOTOX output table by using the scroll bars located at
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the right of computer screen; the top and bottom scroll bar moves left and right in the
output table.
Selection Box and Types
All search and report selections will be displayed in a box using multiple selection
methods (radio button, checkbox, drop-down lists, typing in text (one entry per line)).
Green buttons are used for general changes when new items are applied. Red buttons
are used for removing or resetting information. Blue buttons are used for general
functionality and switching among sections in ECOTOX.
Users must search at least one parameter. Any additional selections made using
multiple parameter search boxes within 'Search' or 'Explore' page will narrow the
search result.
Key Functions
There are some functions that can aid in searching. These Key Functions include:
Update Search - Updates query to most recently selected parameters in "Search' page
Reset All - Erases previously selected search criteria on all search pages and restores
the default report format.
Aquatic Button - Loads or filters to only Aquatic results.
Terrestrial Button - Loads or filters to only Terrestrial results.
Customize Output Fields - A popup window that allows users to change or add data fields
to display in the search results. Note: option will only be available once search has
been conducted.
Export as... - A drop-down menu which allows Excel, csv or Delimited as export types
for results.
View All Applied - A popup window showing parameters applied to specific search.
Users can export this criteria list for documentation purposes.
Condensed (Excel Only) - Under the Settings icon next to "Export as...users
can choose to have full output version of test results by deselecting the checkbox
next to "Condensed". The default is that "Condensed" is selected and all values
appear in a separate column of the report.
References - By selecting the references buttorlSi users can view and export all
reference citations for the data searched.
Options for Searching in ECOTOX
'Search' provides a broader range of search parameters than those available in
'Explore'. The default for each selection box is All Data will be searched. As
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ECOTOX 5.3 User Guide
Selections are added to Search criteria, the number of records that can be retrieved
from the ECOTOX Knowledgebase may be reduced.
Users must search at least one parameter. Any additional selections made using
multiple parameter search boxes within 'Search' or 'Explore' page will narrow the
search result.
ECOTOX offers the following search options:
Search Page Menu: The home page under the 'Search' page provides an overview
of how to navigate within 'Search' and has a navigational tool bar located at the left of
the page, which allows users to navigate to different search parameters (All
Chemicals, Effects, Endpoints, Species, Test Conditions, Publication Options).
All Chemicals - Specific or Multiple Chemical Entry using either the Chemical
Abstract Services (CAS) Registry number or chemical name to identify the
substance(s) or Predefined Chemical Groups
All Effects - Specific or Multiple Effects or Measurements
All Endpoints- Specific or Multiple Concentration Based endpoints, Time Based
Endpoints, Bioaccumulation/Bioconcentration Factor Endpoints, or No
Endpoints
All Species - Specific or Multiple Taxonomic Entry using either the scientific name,
common name, or ECOTOX species number to identify the organism(s) or
Predefined Taxonomic Groups
All Test Conditions - Test Location, Exposure Media, Exposure Type, Control Type,
Chemical Analysis
All Publication Options- Author, Reference Number, Publication Year,
Independently Compiled Data and Recent Modifications/Additions
As selections are made, they will appear in the left frame navigation tool. Users may
also review search selections using the "View All Applied" button after "'Update
Search'" has been applied. To return to the default search parameters, select 'Reset
All'.
The ECOTOX 'Search' function is designed to lead users through a search session
using multiple forms. The left frame provides a menu and navigational search forms
containing search parameter selections. Users must use the navigation tool bar to
move from form to form within 'Search.'
Each parameter selection box also includes a 'Reset'button in the upper right-hand
X Reset
corner of the box. When you click on 'Reset', all selections within the box
are removed, however selections remain in the other boxes. Note: Using your
browser's Back button will result in the loss of all entries made in any of the
ECOTOX forms.
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'Search' selection types include:
Any Test Locations
i ; Lab
Not Reporred
Ail Pieici Tests
Heid, Artificial
^eid, Natural
Uncieiermirsble
Checkbox: To select an item, click on the checkbox to include. To remove a selection,
click on the checkbox again. One or more items may be selected.
Radio button: To select an option, click on the radio button box to include. To unselect,
click on the radio button again. Only one option listed may be chosen.
< Species *Reset
Specific Species
. Q.
~Tfc Copta^s Exac:
Kingdom For Name Searches
¦ js.'Sperirsf's.sm*
1 ^isrts ' ' Cciv-non bme
13th Ta.;orom.c Nsnies
Drop-down List: To modify searches using the drop-down list, click on the arrow icon on
the right side. Clicking on this icon drops down a list immediately below the field and
shows which values can be chosen. Click on the entry item to select.
-• Publication Options
Publication Years
»91S Xf fO 201 < V
Author(s): , -i
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ECOTOX 5.3 User Guide
Text Entry: Chemical, Effects, Species, Author, or Reference Number searches may
require typing the search criteria into the selection box. Each entry must be on a single
line, followed by clicking "Enter" on keyboard. The text must match the type of data
within ECOTOX, either as a sub-string search (Contains) or exactly (Exact Match).
Users may enter both text and numeric data into text boxes.
< Chemicals *
50000
121755
diaxin
cupric chloride
O Contains O Exact Match
Enter each chemical name and/or CAS registry number on separate Sines,
View/Edit: For predefined groups within the 'Search' parameters, users can view and/or
further edit selected lists after "Update Search" has been completed. To access the
view and edit feature, click on the button located at the bottom for the search box
labeled "View/Modify Entries for Selected Groups." This displays a popup window with
detailed list(s) for selections. Changes can be made by deselecting any search
selection and clicking on 'Save Modifications', and selecting 'Update Search' to change
the data output. A 'Restore All' link is also provided to return the parameter to the
default selections, or select the 'Cancel' button to return to original search parameters.
If you click on your browser "Back" button, your edits will not be saved
Explore Page Menu
The 'Explore' function supports searches for Predefined or Custom groups for
Chemicals, Species or Effects Groups. All query filters are available within the
'Explore' menu, but sort order or data field display within the report cannot be
modified. The query options within 'Explore' are also available in 'Search' , but the
'Search' contains more search and output options.
A directory path noting the location in the 'Explore' function appears in the top banner. Users can
move up the path by closing the level using the ^3 button. Selecting the back button returns
you to the main 'Explore' page.
< Explore
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ECOTOX 5.3 User Guide
• Chemical: Within the Chemical Entry search box, users may select any or
multiple Chemicals Groups to explore available data.
• Species: Within the Species Group, users may select any or multiple Species
Groups or develop a custom list to explore available data.
• Effects: Within the Effects Group, users may select any or multiple Effect Groups
in the Group list to explore available data.
ECOTOX DATA SEARCHES
To retrieve ECOTOX data, select either the 'Search' or 'Explore' on the Home page
banner. The query page will then load, however transferring between the 'Search' and
'Explore' functions will lose your current search strategy.
USING THE "SEARCH" FUNCTION TO LOCATE DATA
Chemical Searches
Under the 'All Chemicals' search tab, users can conduct queries on CAS
Registry numbers, partial or complete chemical names, and predefined groups
of chemicals. The default within ECOTOX is that all chemicals are selected for
searching. All chemicals within ECOTOX include a CAS Registry number and a
chemical name, typically a Collective Indices name. This information is verified
in reliable sources. Appendix D describes the verification process for chemicals
in the ECOTOX system. The Collective Indices name is identified as the
preferred name within the ECOTOX Knowledgebase, and this is the name
displayed on the report even if the search was conducted using a common or
trade name of a substance. ECOTOX includes chemical synonym searching as
an option; however, if a synonym is used by more than one CAS number, both
chemicals will be queried.
Chemical Entry
To conduct a search, type in the CAS Registry number(s) and/or chemical name(s).
Users may enter the CAS Registry number with or without hyphens and leading zeroes.
CAS number queries are always exact matches.
Users can search for an unlimited number of entries and each entry must be entered on
a separate line and users can mix numbers and name entries.
Chemical Name: Users can now search ECOTOX for chemical synonyms. Enter the
names of the chemicals, placing each name in a separate field.
Note: If the term benzene is entered with the "Contains" radio button, all
chemicals that contain the sub-string benzene will be retrieved. Selecting the "Exact
Match" will return only results for benzene. It is recommended to search on CAS
Registry numbers (CASRN) to specifically restrict search to selected chemical(s).
Chemical Dashboard: On the 'All Chemicals' searching parameter, a link directs users
to the EPA's "CompTox Chemicals Dashboard" to search by CASRN for more
information on a chemical.
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ECOTOX 5.3 User Guide
Note: CAS Registry number with or without hyphens made be entered for
ECOTOX searches, but exact CAS Registry number with hyphens is preferred for the
CompTox Chemicals Dashboard searches.
Predefined Chemical Groups
The option to select from predefined lists is available in the 'Search' function. Chemical
lists have been provided to effectively search a variety of Metal/Organometal or Organic
compounds chemical groups. To select a chemical group or specific chemical(s) of
interest, click on the checkbox to search. To unselect, click on the checkbox again.
Search Tips for Chemicals
Some guidance when conducting a chemical search:
Metal Compounds: It may be more effective to search metal compounds by chemical
name, i.e. entering cupr and copper as chemical names will find copper and several
copper compounds with fewer keystrokes than typing all the individual CAS Registry
numbers. Users may also search a group of copper compounds using the ECOTOX
Predefined Chemical Group option.
Organic Compounds: These compounds may be searched by chemical name, i.e.
entering dioxin as a chemical name will be more efficient than entering all the specific
dioxin chemical names or CAS Registry numbers. Remember, though, entering some
chemical names may identify many non-applicable chemicals (e.g., benzene will result
in all compounds with the sub-string 'benzene' in the chemical name), therefore it is
recommended to search by CAS Registry number if a specific chemical is required.
Pesticides: Pesticides are usually found by typing the common synonym name or trade
name. Chemical CAS Registry numbers may be located via link to EPA's "Chemicals
Dashboard" or other chemical indexing resources.
Search bv Effect Measurements
Under the "All Effects" search tab, users can conduct queries on specific effect measurements
(including Post-exposure results), or by predefined groups of effects.
Note : If the term vitellogenin is entered with the "Contains" radio button, all effects
that contain that sub-string will be retrieved. Selecting the "Exact Match" will return
only results for vitellogenin.
The default within the ECOTOX Knowledgebase is that All effects are selected for searching. All
coded effects are categorized into one of 11 major effect groupings such as Accumulation,
Growth, Mortality, etc.
Each effect includes a list of observed measurements. For instance, the Effect
Measurement "Biochemical Group" includes three effect categories: biochemical,
enzyme, and hormone. Within each of these effects there are multiple measurements
located under "View/Modify Entries for the Selected Group(s)".
For further refinement of observed effect information, users may click on the
"View/Modify Entries" button located at the bottom of the 'Effect Measurements'
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ECOTOX 5.3 User Guide
selection box. A new window will open and display the list of specific measurements for
each of the selected effect(s) and/or effect group(s). Measurements include quantitative
observations that describe and evaluate biological responses to toxicants. Each effect
(e.g., Growth) can have several associated measurements (e.g., length, weight). The
ECOTOX Term Appendix located in the "What Is..." section of the Help web page
provides definitions of the effect measurement codes used in ECOTOX.
The 'View/Modify Entries' window allows users to view and edit Effect Measurements to
include in search. To remove a specific measurement, click on the highlighted blue box
to remove the effect, and more than one measurement may be removed at a time.
Measurements may be restored by clicking on the "Restore AN" default button.
Note: If only a few effect measurements are desired, select "Clear AH" and reselect only
those measurements of interest. When complete, click on the "Save Modifications"
button to close the window and return the 'Search' page. If you click on "Back" button,
edits will not be saved.
Select "Update Search "to apply changes to the output.
Post Exposure Measurements: Within the "All Effects" tab, this option allows users to
exclude results of responses observed during a post exposure period. If a study
includes observations of organisms after the chemical exposure has been
discontinued, these measurements are identified as 'Post Exposure Measurements'
and are included in the search results.
Records for Post Exposure Measurements can be recognized by clicking the
'Customize Output Fields' button and selecting the 'Display lookup codes instead of
descriptions' checkbox at the bottom of the pop-up window. Post Exposure
Measurements will be displayed with a tilde (~) in front of the Effect Code. For example,
a post exposure measurement mortality effect would have ~MOR for the Effect Code.
Note: When using the 'Explore' feature, Post Exposure Measurements are also
automatically included in a query but currently cannot be separated.
Note: Differences in record number between 'Search' and 'Explore' could be the result
of the way each function handles the Delayed Effects records (in 'Search', they need
to be explicitly searched; however, with Explore, they are automatically included).
Search bv Endpoints
Under the "All Endpoints" search tab, users can conduct queries on any or all
specific endpoints or by predefined group of effects.
The default within the ECOTOX Knowledgebase is that "All Endpoints" are selected for
searching. For the purposes of ECOTOX, an endpoint is defined as the quantification of
an observed effect obtained through statistics or other means of calculation for the
express purpose of comparing equivalent effects (e.g., LC50).
For each toxicity test record, pertinent information on test results presented by the
authors are encoded within the database. Endpoint information is coded if it is reported
by the author.
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Within the "Any Endpoints" tab, users can choose one or more endpoints within the
checkbox by clicking to select endpoints to include in query. The Endpoint selection
box is divided into the following sections: Concentration-Based or Time-Based
Endpoints, Bioaccumulation / Bioconcentration Factors (all statistically-derived, or
calculated as reported by the author or determined by the ECOTOX staff), or
Statistics, No Endpoint (which contains statistically analyzed data but authors did not
identify a specific endpoint or one could not be determined by ECOTOX staff) or
Endpoint Not Reported (NR) where data was not statistically analyzed.
The "View All Applied" box allows users to view and export all search parameters.
More endpoints can be selected after conducting the initial search, but users must
select "Update Search" to apply changes to the output.
Search bv Taxonomy
Under the "All Species" search tab, users can conduct a search by using Species
Names or Number(s), or by using Predefined Species Groups. All data records within
ECOTOX include a Scientific name for the test species. All names have been verified
in reliable taxonomic sources. Appendix D contains information regarding the
verification of species data in ECOTOX.
The ECOTOX species file includes historical synonyms for the species. If a search is
conducted using a species name that is noted as a taxonomic synonym in our system,
ECOTOX Knowledgebase will present the results using the currently acceptable genus
and species name.
Taxonomic Entry
Users can search for an unlimited number of species entries, but each entry must be
entered on a separate line or separated by a comma. Users can mix numbers and
name entries, but the name entry must be the same type (e.g., Genus/species and
Common names cannot be searched within one entry screen).
Genus/Species Name: Users can conduct a search on whole or fragments of
scientific names (Genus, Species).
Species Common Name: All data records within ECOTOX include a common name for
each species. Users can conduct an exact search (Exact Match) on the common
name or fragments.
Other Taxonomic Names: Key taxonomic levels (Kingdom, Phylum, Class, Order,
Family) searches are available by typing the appropriate scientific name.
Note: If a species name is entered, users can select either the "Contains" or the
"Exact Match" radio buttons. For example, if users enter trout and select the
"Contains" radio button, it will return everything that contains the sub-string trout.
For name searches, users are required to select the type of name (e.g.
Genus/Species name, etc.); If trout is entered, users would need to select the
Species Common Name radio button to retrieve any results. Selecting the "Exact
Match" is best utilized when the literal string (exact name) of the species is
known (e.g. rainbow trout or Daphnia sp.)
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Predefined Species Groups
Species lists have been provided to effectively search a variety of species groups.
For further refinement of a species group, users may click on the "View/Modify Entries
for Selected Group(s)" button located at the bottom of the Species selection box. A new
window will open and display the list of specific species for each of the selected groups.
The View/Modify entries window allows users to view and edit species to include in
search. To remove one or many species, click on the highlighted blue box to remove
the species. Users may restore species by clicking on the "Restore AN" default button.
If only a few species are desired, select "Clear AN" and reselect only those species of
interest. When completed, click on the "Save Modifications" button to close window
and return the 'Search' page. Clicking on browser "Back" button will remove all edits.
Select "Update Search" to apply changes to the output.
Note: Users cannot display both the Animal and Plant species groups due to the large
number of species within these lists.
Taxonomic kingdom (plant or animal) searching is available in 'Search'. The kingdom is
searched using a radio button option located within "AN Species" as "Plants" for the
plant kingdom or "Animals" for the animal kingdom.
The Plant kingdom search also includes species representing Monera and Fungi. Some
test results report both plant and animal species as one effect measurement (e.g.,
aquatic community, plankton, soil community). These results will be included when
either plant, animal or both kingdoms are selected.
Search Tips for Taxonomic Searches
By clicking on "AN Species" on the frame at the left of the search page, will move
users to the Taxonomic Search area. Some examples are provided to help when
developing your search strategy:
Genus/Species Name: Entering Pimephales promelas in the search text box will result
in only data for fathead minnows. Entering daphnia genus as the genus/species name
will result in all Daphnia and Ceriodaphnia species. If a specific genus and/or species
names is consistently searched, a user may want to use the species number for
searching (ECOTOX species number or NCBI TaxlD number).
Users may also enter a historical Scientific name and still retrieve data for a species.
For example, if Salmo gairdneri is entered, the output will display the currently
accepted name, Oncorhynchus mykiss.
Other Taxonomic Name: Any taxonomic level (Kingdom, Subphylum, Phylum
(Division), Superclass, Class, Order, Family, Genus) can be searched using this
Taxonomic Entry box. For example, entering salmonidae will retrieve all species for
this family.
Species Common Name: Using some common names may be an effective way to
search if there is a unique common name for that organism. For example, entering
mallard in the common name field will result in only mallard duck results.
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Note: Entering the term duck will output results for duck and duckweed. In this case,
searching using the common name (exact) or performing only performing the query
on terrestrial data will eliminate the duckweed from the search.
Note: Entering bird in the common name field will result in bird and ladybird beetle
data. In addition, using the term birdmW not ensure that all bird data in the system will
be extracted because the species name may not use the term bird in the common
name.
Species Number: The species number is the unique indexing number assigned to each
species in ECOTOX and can be used as a shortcut method to search genus and/or
species data.
Search bv Test Conditions
For each toxicity test record, pertinent information on testing procedures presented by
the authors are documented within the database. Search selections available on this
page are Test Location, Exposure media, Exposures types, Control types and Chemical
analysis.
The options for searching by test conditions are briefly described below.
Test Location
The valid entries for test location are Lab (laboratory), Field (all outdoor field tests,
artificial, natural or undeterminable) and Not Reported (i.e., the author(s) did not present
sufficient information to determine test location). The default within ECOTOX is that all
data, regardless of test location, are included in search result. To selectively search on
a specific test location, click to mark the appropriate checkbox.
Exposure Media
The default within ECOTOX is that all data, regardless of test media, are included in
search result. To selectively search on a specific exposure type, click to mark the
appropriate checkbox.
Aquatic freshwater tests include those conducted in freshwater, reconstituted water,
distilled water, or tap water. Saltwater tests include those conducted in natural or
artificial seawater, brackish water, or estuarine water. Not Reported (NR) is used if a
determination cannot be made regarding the use of either freshwater or saltwater.
Terrestrial exposure media selections are focused on tests using a substrate (e.g., soil
or artificial media). If the terrestrial organism does not utilize a substrate for nutrition
(e.g., birds, mammals), do not select any exposure media types.
Exposure Type
Users can select the exposure type by clicking the items in the search selection box area.
Organisms are typically exposed to toxicants through aqueous, diet, injection, in vitro,
topical or environmental routes. Occasionally, an exposure may be through multiple
routes (e.g., such as topical and oral).
ECOTOX includes chemical exposures on whole living organisms as well as organisms
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that are not intact (in vitro). For example, in vitro exposures can contain studies using
excised organs and cell cultures. Inclusion of in vitro studies into ECOTOX have the
same species criteria requirements as those for whole organisms (ecologically relevant
and exclude humans, monkeys, rats and mice).
Control Type
Control Type allows the capability to filter test records based on author reported test
control types, as well as the ability to select specific control type(s).
Individual control types may be selected by choosing the appropriate checkbox(es)
from the following options:
o Baseline (B) - parameters measured before administration of test chemical
o Concurrent (C) - run simultaneously with the exposure
o Historical (H) - data collected often during a long-term survey of the area
o Multiple (M) - multiple type of controls reported, noted individually
o Other (0) - control is run in a different system than exposures
o Positive (P) - an exposure that causes a desired effect in the experiment
o Solvent (V) - exposed to carrier or solvent only
o Undefined (K) - control is presented but without accompanying
methodology
Historical ECOTOX Control Types
o Insufficient (I) - not enough information presented to determine control type
o Multiple Controls (OK) - multiple type of controls reported
o Satisfactory (S) - run in the same system, low mortality
o Unsatisfactory (U) - chemical entered control, high mortality
Control Not Reported
o No Control Used (Z) - authors state no control used
o Not Coded (NC) - was not coded
o Not Reported (NR) - was not reported
Chemical Analysis
The method of chemical analysis filters test records, based on the author reported
chemical concentrations, as measured or nominal values. Individual chemical analysis
types may be selected by choosing the appropriate checkbox(es) from the following
options
Measured: Exposure and/or observation concentrations or doses are quantitative;
analysis methods may be reported.
Unmeasured: Exposure and/or observation concentrations or doses are clearly
identified as nominal values; or when the author does not report whether the
concentrations were measured or nominal, i.e., unmeasured is used as a default value
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when there is no information provided about the reported chemical concentrations.
Not Reported: Exposure and/or observation concentrations or doses are not reported.
Search bv Publication Options
Publication Year(s)
The default within the ECOTOX Knowledgebase is that all data, regardless of
publication year, are included in search result. The default publication year search may
be overridden by selecting a range of publication years.
The Aquatic component of ECOTOX contains data from publication years 1915 to
present; the Terrestrial component of ECOTOX contains data from publication
years 1926 to present.
Author(s)
Searches may be conducted on specific authors. To search on an author, enter the
specific author names in the selection box while in the "All Publication Options" of
the 'Search' page, one author per line. Partial author names may be used. Only
authors which have data extracted in the ECOTOX Knowledgebase are returned.
Reference Number
Each publication abstracted for the ECOTOX Knowledgebase is assigned a unique
reference number. These reference numbers are available for selection for any
ECOTOX outputs. To conduct a search, enter a valid ECOTOX reference number(s) in
the selection box in the "Ref Num" box, with one reference number per line.
Independently Compiled Data
ECOTOX includes several independently compiled data sets. Data sets from the
Organization for Economic Cooperation and Development (OECD), Russia, Office of
Pesticide Programs, the U.S. Geological Survey, and MED are included as subsets of
the ECOTOX Knowledgebase. For further information on these data files, refer to
Appendix F.
Recent Modifications and Additions
The default within ECOTOX is that all data, regardless of the date added to the
database, are included in search results. Data may be restricted to just newly updated
or modified data using this filter. The "Recent Modifications/Additions" search box
allows searches based on the last 10 updates to the Knowledgebase, which typically
span two to three years. This feature is useful for specific queries (e.g., list of species
and/or chemicals) conducted on a regular basis.
Users can select "View All Applied" box to view the selected parameters used in the
'Search' function. This information can also be exported to retain a dated record of
each search.
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Output and Report Format Options
Within 'Search' function, the output table report has been condensed to include only the
most utilized output fields. All or some of the coded fields can be exported by first
selecting "Customize Output Fields" button, then click on "Select All" or clicking on any
additional fields to display in search results. All updated fields will be displayed in the
output table.
Navigating within Output table: Users can move through the report in several ways.
To view within a page, use the scroll bar on the right side of the window. To view all
coded output fields, scroll down to bottom and then scroll to right of table. To move
from one page to another page of the report, use the numbered hyperlinks located at
bottom of each report page.
Users can also choose to export data to an Excel spreadsheet or delimited file. The
report will be saved as either Aquatic or Terrestrial depending on selected output.
In the downloaded/saved Excel report, if the author did not report data for a database
field, the output report will display an NR or 'Not reported.' Definitions for any codes
presented in the report can be found in the "ECOTOX Term Appendix" located under
the "Help" tab.
Note: Users must first export output to Excel or delimited file; currently users cannot
print directly from the HTML output.
Delimited Output
Users are able to generate an ASCII delimited data file of search results. The delimited
tabular output format has set default output fields. If the author did not report data for a
parameter, the delimited output will have an NR or 'Not reported' in the data field.
Definitions for all terms presented in the report can be found in the ECOTOX Term
Appendix located in the "Help" section under the Home page.
The exported data file may be imported into spreadsheet or database software for
further refinements. Each data element is separated into a unique field, and each test
record appears on a single line. For delimited reports, the Reference Citation field will
appear in the separate delimited fields (Reference Number, Author, Title, Publication
Year and Source).
Note: Each field in the delimited file report will be separated by a vertical bar ("|").
Users may have to specify the vertical bar as the delimiter when importing the data. A
forward slash (/) within a field refers to an associated comment. Comment fields can be
selected in output to help interpret unique test conditions but for a complete
understanding of the toxicity study, refer to the full publication.
Display Fields
Select data parameters are presented in the default versions of the Aquatic and
Terrestrial tabular reports. A checkmark appears in data fields that are displayed in the
default output format. Users can change the display fields for Excel and delimited
outputs by selecting the "Customize Output Fields" button to add additional output fields
then select "Update Search". To remove a selected data field, click on the checked box.
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Click on the "View All Applied" to see modified entries. Full Data Record outputs cannot
be modified.
USING THE "EXPLORE" FUNCTION TO LOCATE DATA
About Explore
The 'Explore' function is a great tool for searching the ECOTOX Knowledgebase if the
exact parameters to search are not known or a visual representation of general and
specific data trends is desired. It is an interactive way to examine search paths by
Chemical, Species and Effects. There are ways to examine the data visually via
plotting functions.
Explore bv Chemical
Predefined Chemical Groups
The option to select from predefined lists is available in the 'Explore' function.
Chemical lists have been provided to effectively search a variety of
Metal/Organometal or Organic compounds chemical groups. To select a chemical
group or group(s) of interest, click on the checkbox you want to search. To unselect,
click on the checkbox again.
Searches for data on a specific user-defined single chemical or "custom group" of
chemicals can also be conducted from the Explore chemical page, however users
cannot select both a chemical from the pre-defined group list and enter in a Custom
group chemical. After a specific group is selected, click on "Explore Data" button.
Explore Data >
The default within the ECOTOX Knowledgebase is that all chemicals in the selected
group are selected for searching. The output can be refined by filtering the tabular data
using the text entry fields at the top of each column to limit results to specific chemicals
in that group, or by using the Query filters on the left side of table.
Notice that the initial output has both Aquatic and Terrestrial data reported under the
Group Summary tab. Users can deselect one or the other by clicking the checkbox to
filter data. Any specific Chemical on the output table can be selected for viewing by
clicking on the green arrow on right side of output table . This will refine the output
table to all records by selected chemical.
Q Aquatic Q Terrestrial Records Plot View
Users can filter all available data results by selecting the "Records" tab above the
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table to show all Distinct records in the Group.
] Aquatic Q Terrestrial Group Summary Plot View
Note: There are fewer output columns displayed in the 'Explore' feature as
compared to the 'Search' function. To see all available data for specific results,
users can send the query filters and search parameters to the 'Search' function of
the Knowledgebase using the green button on the upper right of the Records
Table.
Send Query Filters to Search
Refining/Filtering data using Query filters
Users can refine tabular data using filters on the left side of the table. All chemicals,
species, and effects data resulting from an initial search can be refined via the
dropdown boxes under each filter group. Users may select one or multiple options in
each box. Once filters are selected, click 'Enter' on keyboard or mouse-click outside
the box to apply to data output table. Users can select "View All Applied" box to view
the selected parameters. This information can also be exported to retain a dated
record of each search.
Data Visualization Plots
Data output presented in the Explore table can also be plotted (if applicable) by
selecting the 'Plot View' tab.
Aquatic Q Terrestrial Group Summary Records
Plot View
Currently, only Aquatic data or Terrestrial data that can be converted to ppm is
viewable in the Plot View, and there are three types of plots available: Effect by
Chemical, Duration by Chemical and Duration by Endpoint.
The data presented in the table or in the plot can be further refined using either the
query filters on the left side of the webpage, or via the headers in the output table.
Users can also "zoom' into the plot to refine output, or by deselecting/selecting items in
plot legend.
Hovering the cursor over any of the data points in the graph will highlight the study.
Clicking on any data point in the plot will highlight the result in the table below the plot.
Notice in the 'Explore' plot table, only 13 output columns are shown. To see all available
data for specific results, the search parameters must be used in the 'Search' function of
the Knowledgebase (see above section).
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Explore bv Species
Predefined and Custom Species Groups
The option to select from predefined group or a Custom Group is available in the
'Explore' function. Species groups have been provided to effectively search a variety
of Animal, Plants, or Special interest groups. To select one or many species group(s)
of interest, click on the checkbox(es) to search. To unselect, click on the checkbox
again.
After specific group is selected, click on "Explore Data" button.
Explore Data >
The default within the ECOTOX Knowledgebase is that all species in the selected group
are selected for searching. Users can limit results to specific species by filtering tabular
data using the text entry fields at the top of the table columns, or by using the query
filters on the left side of table.
Notice that the initial output has both Aquatic and Terrestrial data reported under the
Group Summary tab (if applicable). Users can deselect one or the other by clicking the
checkbox to filter data. Any specific Species on the output table can be selected for
viewing by clicking on the green arrow on right side of output table . This will refine
the output table to all records by selected species,
Q Aquatic Q Terrestrial Records Plot View
Users can look at specific data results by selecting "Records" tab above the table
which will show all Distinct records in the Group:
Q Aquatic Q Terrestrial Group Summary Plot View
To create a custom group of species, select "Create Custom Group" button from the left
side panel.
Create Custom Group...
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From there, users can either "Browse Species" by inputting a common name, Latin
name or NCBI TaxlD and selecting one or more species, or chose to "Enter by NCBI
TaxID." Users can also copy and paste a list of NCBI TaxIDs into the appropriate box.
Note: There are fewer output columns displayed in the 'Explore' feature as
compared to the 'Search' function. To see all available data for specific results,
users can send the query filters and search parameters to the 'Search' function of
the Knowledgebase using the green button on the upper right of the Records
Table.
Refining/Filtering data using Query filters
Tabular data can be refined using filters on the left side of table. All chemicals,
species, effects, or other data present from the initial search can be refined via the
dropdown boxes under each filter group. Users may select one or multiple options in
each box. Once filters are selected, click 'Enter' or mouse-click outside of the box to
apply to data output table. Users can select "View All Applied" box to view the selected
parameters. This information can also be exported to retain a dated record of each
search.
Data Visualization Plots
Data output presented in the Explore table can also be plotted (if applicable) by
selecting the 'Plot View' tab.
] Aquatic Q Terrestrial
Group Summary Records
Plot View
Currently, only Aquatic data or Terrestrial data that can be converted to ppm is
viewable in the Plot View, and there are three types of plots available: Effect by
Chemical, Duration by Chemical and Duration by Endpoint.
The data presented in the table or in the plot can be further refined using either the
query filters on the left side of the webpage, or via the headers in the output table.
Users can also "zoom' into the plot to refine output, or by deselecting/selecting items in
plot legend.
Hovering the cursor over any of the data points in the graph will highlight the study.
Clicking on any data point in the plot will highlight the result in the table below the plot.
Notice in the 'Explore' plot table, only 13 output columns are shown. To see all available
data for specific results, the search parameters must be used in the 'Search' function of
the Knowledgebase (see above section).
Explore bv Effects
Predefined Effects Groups
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The option to select from predefined lists is available in the 'Explore' function. Effects
groups have been provided to effectively search a variety of typical apical effects such
as Growth, Reproduction and Mortality, but also include newer effects groups such as
Behavior and Physiology effects. To select one or many species group(s) of interest,
click on the checkbox(es) to search. To unselect, click on the checkbox again.
After specific group is selected, click on the "Explore Data" button.
Explore Data >
The default within the ECOTOX Knowledgebase is that all effects in the selected group
are selected for searching. Users can limit results to specific effects by filtering tabular
data using the text entry fields at the top of the table columns, or by using the query
filters on the left side of table.
Notice that the initial output has both Aquatic and Terrestrial data reported under the
Group Summary tab (if applicable). Users can deselect one or the other by clicking the
checkbox to filter data. Any specific Effect on the output table can be selected for
viewing by clicking on the green arrow on right side of output table . This will refine
the output table to all records by selected effect.
Q Aquatic Q Terrestrial Records Plot View
Users can look at specific data results by selecting "Records" tab above the table
which will show all Distinct records in the Group.
Q Aquatic Q Terrestrial Group Summary Plot View
Note: There are fewer output columns displayed in the 'Explore' feature as
compared to the 'Search' function. To see all available data for specific results,
users can send the query filters and search parameters to the 'Search' function of
the Knowledgebase using the green button on the upper right of the Records
Table.
Refining/Filtering data using Query filters
Tabular data can be refined using filters on the left side of table. All chemicals,
species, effects, or other data present from the initial search can be refined via the
dropdown boxes under each filter group. Users may select one or multiple options in
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each box. Once filters are selected, click 'Enter' or mouse-click outside of the box to
apply to data output table. Users can select "View All Applied" box to view the selected
parameters. This information can also be exported to retain a dated record of each
search.
Data Visualization Plots
Data output presented in the Explore table can also be plotted (if applicable) by
selecting the 'Plot View' tab.
Aquatic Q Terrestrial Group Summary Records
Plot View
Currently, only Aquatic data or Terrestrial data that can be converted to ppm is
viewable in the Plot View, and there are three types of plots available: Effect by
Chemical, Duration by Chemical and Duration by Endpoint.
The data presented in the table or in the plot can be further refined using either the
query filters on the left side of the webpage, or via the headers in the output table.
Users can also "zoom' into the plot to refine output, or by deselecting/selecting items in
plot legend.
Hovering the cursor over any of the data points in the graph will highlight the study.
Clicking on any data point in the plot will highlight the result in the table below the plot.
Notice in the 'Explore' plot table, only 13 output columns are shown. To see all available
data for specific results, the search parameters must be used in the 'Search' function of
the Knowledgebase (see above section).
Exporting Data in Explore
0 Export CSV
Tabular data output can be exported to CSV file by clicking on button and
saving file to computer.
If the number of records is larger than expected, users may close the report window
and return to the ECOTOX Knowledgebase window to refine search strategy, or
conduct another search by clicking the "Reset AN" button.
EXITING ECOTOX
Exiting the web browser will leave the program. Exiting the web browser will not save
any searches.
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APPENDIX A: ECOTOX SEARCH PLANNING FORM
Use this form to help plan searches or to document searches for others to perform.
Chemicals
Chemical Names
CAS
Numbers
Predefined Groups
Metal Compounds
Organic Compounds
Aluminum
Conazoles
Antimony
DDT and metabolites
Arsenic
Dibenzofurans
Barium
Explosives
Beryllium
Cadmium
Glycol Ethers
Major Ions
Neonicotinoids
Chromium
Cobalt
Nitrosamines
Copper
Perchlorates
Iron
Phthalate Esters
Lead
Polyaromatic Hydrocarbons (PAH)
Manganese
Mercury
Polychlorinated Biphenyls (PCB)
Polybrominated Diphenyl Ethers (PBDE)
Nickel
Pharmaceutical Personal Care (PPCP)
Organotin
Selenium
Silver
Perfluorooctane Sulfonates/Acids
Vanadium
Zinc
- (PFAS/PFOS)
Species
Scientific Names/
Taxonomic Levels
Common Names
Species
Numbers
Predefined Taxonomic Groups
All Animals
Amphibians
Insects/Spiders
Molluscs
Birds
Other Invertebrates
Reptiles
Crustaceans
Mammals
Worms
Fish
All Plants
Algae, Moss/Hornworts, Fungi, Flowers,
Trees, Shrubs, Ferns
Special Interest
Standard Test Species
US Threatened/Endangered Species
US Exotic/Nuisance
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Test Results
Endpoints:
Concentration Based
Time Based
Bioaccumulation/bioconcentration Factor
Statistics, No endpoint
Effect Groups:
Accumulation Mortality
Behavior Physiology
Biochemical Population
Cellular Reproduction
Growth Ecosystem
Specific Effect Measurements
Include Delayed Effects
Test Conditions
Test Location(s):
Lab
All Field
Tests
Field Artificial
Field Natural
Field Undeterminable
Exposure Media:
WATER: Freshwater
Saltwater Not Specified
SOIL: _ Artificial
Humus Litter Manure Mineral Soil Natural Soil
Unspecified Soil
Soil Mixture
ARTIFICIAL: Hydroponic
Other
Exposure Tvpe:
Diet
Flow-through (aquatic)
Injection
Leaching (aquatic)
Topical
Intermittent (aquatic)
Environmental
Renewal (aquatic)
Multiple Entry
Lotic (aquatic)
In-vitro
Static (aquatic)
Not Reported
Lentic (outdoor aquatic)
Tidal (outdoor aquatic)
Control Tvpe:
Concurrent
Insufficient
Multiple
Multiple Controls
Baseline
Satisfactory
Solvent
Unsatisfactory
Positive
No Control
Historical
Not Coded
Undefined
Not Reported
Other
Chemical Analysis:
Measured Unmeasured Not Reported
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Publications
Publication Years:
Author:
Reference Number(s):
Independently Compiled Data:
EPA: Fathead Minnow Acute Toxicity
EPA: Office of Pesticide Program Database
Dutch Dataset:
French Dataset:
German Dataset:
Russian Dataset:
USGS Acute Toxicity Dataset:
Recent Update Dates:
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Report Output
Aquatic Output Elements
Standard default output elements are listed in bold. Some output options
are available for Field Data only and are indicated by (Field Only).
Terrestrial Output Elements
Standard default output elements are in bold. Some output options
are available for Field Data only and are indicated by (Field Only).
Alkalinity
Application Date (field only)
Application Date (field only)
Application Date /Season (field only)
Application Date /Season (field only)
Application Frequency
Application Frequency
Application Rate (field only)
Application Rate (field only)
Application Type (field only)
Application Type (field only)
Author
Author
CAS Number/ Chemical Name
BCF Value
Chemical Analysis Method
Calcium
Chemical Carrier
CAS Number
Chemical Comment
CAS Number/ Chemical Name
Chemical Formulation
Chemical Analysis
Chemical Grade
Chemical Carrier
Chemical Half Life (field only)
Chemical Comments
Chemical Purity
Chemical Formulation
Chemical Radiolabel
Chemical Grade
Control Type
Chemical Half Life (field only)
Dose (Author)
Chemical Purity
Dose Number
Chemical Radiolabel
Dose Statistical Method
Chlorine
Doses
Concentration (Author)
EE Comment
Concentration (Standardized)
Effect
Conductivity
Effect %
Control
Effect Measurement
Dissolved Inorganic Carbon
Endpoint
Dissolved Oxygen
Endpoint Assignment
Doses
Endpoint BCF/BAF
EE Comment
Experimental Design
Effect
Exposure Comment
Effect %
Exposure Duration (Author)
Effect Measurement
Exposure Duration (Days)
Effect /Effect Measurement
Exposure Sample Number
Endpoint
Exposure Type
Endpoint Assignment
Exposure Type/Chemical Analysis method
Endpoint BCF value
Gender
Experimental Design
General comments
Exposure Duration (Author)
Geographic Code (Field Data Only)
Exposure Duration (Days)
Geographic Location (Field Data Only)
Exposure Sample Number
Habitat
Exposure Type
Habitat Code (Field only)
Exposure Type/Chemical Analysis method
Habitat Comment (Field only)
Gender
Intake Rate
General comments
Ionic Fraction
Geographic Code (Field Data Only)
Longitude/Latitude (Field Data Only)
Geographic Location (Field Data Only)
Media Cation Exchange Capacity
Habitat
Media Measurement
Habitat Code (Field only)
Media Moisture
Habitat Comment (Field only)
Media Organic Matter and Type
Hardness
Media Type
Humic Acid
Media Type/Test Location
Intake Rate
Observed Duration (Author)
Ionic Fraction
Observed Duration (Days)
Longitude/Latitude (Field Data Only)
Observed Response BCF/BAF
Magnesium
Organism Age
Media Type
Organism Comment
Media Type/Test Location
Organism Initial Weight
Number of Doses
Organism Lifestage
Number of Doses/Result Sample Unit
Organism Source
Observed Duration (Author)
Other Effects
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Observed Duration (Days)
Publication Year
Observed Response BCF/BAF
Reference Citation
Organism Age
Reference Number
Organism Comment
Response Site
Organism Initial Weight
Response Site/Exposure Duration (Days)
Organism Lifestage
Result Percent Dry/Wet Weight
Organism Source
Result Comment
Other Effects
Result Percent Lipid
pH
Result Sample Number/Unit
Potassium
Result Sample Number
Publication Year
Result Sample Unit
Reference Citation
Significance Level
Reference Number
Significance Level/Statistical Significance
Response Site
Soil Clay %
Response Site/Exposure Duration (Days)
Soil Dose Measured
Result Percent Dry/Wet Weight
Soil pH
Result Comment
Soil Sand %
Result Percent Lipid
Soil Silt %
Result Sample Number/Unit
Soil Type
Result Sample Number
Species Common Name
Result Sample Unit
Species ECOTOX Number
Salinity
Species Final Weight
Season
Species Group
Significance Level
Species NCBI TaxlD
Significance Level/Statistical Significance
Species Scientific Name
Sodium
Species Scientific Name/Species Common Name
Species Common Name
Species Taxonomic Information
Species ECOTOX Number
Statistical Significance
Species Final Weight
Steady State
Species Group
Study Duration (Author)
Species NCBI TaxlD
Study Duration (Days)
Species Scientific Name
Study Type
Species Scientific Name/Species Common Name
Substrate Code (Field only)
Species Taxonomic Information
Substrate Comment (Field only)
Statistical Significance
Temperature
Steady State
Test Comments
Study Duration (Author)
Test Location
Study Duration (Days)
Test Method
Study Type
Test Number
Substrate Code (Field only)
Test Type
Substrate Comment (Field only
Trend
Sulfate
Trend/Effect %
Sulfur
Temperature
Test Location
Test Method
Test Number
Test Type
Trend
Trend/Effect %
Water Depth
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APPENDIX B: PRACTICE SEARCHES
Examples utilizing the 'Search' function page. After each example search,
remember to click on "Reset AN" before proceeding to the next search. All
searches start on the ECOTOX Home page (https://www.epa.gov/ecotox). After
each example, remember to click on "Reset AN" before proceeding to the next
example.
Example A
You want to locate All Reproductive effects data for Nickel compounds. You want to
include the specific habitat information for any results.
1. From home page, click on 'Search'
2. Click on "AN Chemicals" from the menu. Scroll down to "Any Chemical Group".
3. Select Nickel checkbox from the metal compound list.
4. Click on "AN Effects" from the menu. Select Reproduction Group.
Update Search
5. Click on the "Update Search" button.
6. Notice that Aquatic data is displayed, click on "Customize Output Fields"
Customize Output Fields
lin the upper right hand. Scroll down and click on the
Habitat checkbox to add this field to your output. Then click 'Save'.
7. Note: If you wish to view Terrestrial data, click on the Terrestrial button. You will
have to select the same Output fields for the Terrestrial button.
Terrestrial
Example B
You want to locate LC50 data on Freshwater organisms exposed to Malathion.
1. From home page, click on'Search' B9
2. Click the "AN Chemicals" menu on the left frame of the Search page. Type in
CAS Number '121755' or 'Malathion' in the Chemical entry selection box.
3. Click "AN Endpoints" menu. Within the "Concentration Based Endpoint" menu,
select 'LC50' checkbox.
4. Click "AN Test Conditions" menu. Scroll down to the "Exposure Media" selection
box and click on the Fresh Water checkbox.
5. Click on the "Update Search" button.
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Example C
You want to locate recently published, lethality endpoint only studies on Daphnia
magna.
1. From home page, click on 'Search'
2. Click "All Species" menu on the left frame of the 'Search' page. Type in
"Daphnia magna' and confirm that the Genus/Species Name radio button is
highlighted.
3. Click on the "All Effects" menu and select the 'Mortality Group' checkbox.
4. Click on the All Publication Options" menu. Within the "Publication Years"
selection box, select 2010 from the first drop down list and select 2018 from the
second drop down list.
Update Search
5. Click on the "Update Search" button I
6. Note: Only 'Aquatic' data should be presented in the table.
Example D
You want to locate toxicity data for Amphibian tests performed in an outdoor location.
You would like to move these data records into your own database.
1. From home page, click on 'Search'
2. Click on the "All Species" menu on the left frame of the 'Search' page. Scroll
down to the "Any Species Groups" and check the 'Amphibians' checkbox.
3. Click on the "All Test Conditions" menu. Within the "Any Test Locations" area,
select the checkbox 'All Field Tests' from the list. Also, within the "Exposure
Media - Water" select "Fresh', 'Salt' and Not Specified'.
4. Click on the Terrestrial button. (Selecting 'Aquatic' will return too many to view,
but if desired, you could select additional filters to refine output)
5. Click on the "Update Search" button.
Update Search
6. Click "Export As..." button. Choose either 'Excel' or 'Delimited' report option for
the results.
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ADDITIONAL SEARCH and EXPLORE EXAMPLES
Search Example
You want to locate all reproductive effects data for Buprofezin. What types of
reproductive effects were measured?
1. From the ECOTOX home page, click 'Search' on the top banner. HSS
2. Select the 'All Chemicals' tab from the left frame.
3. Type "Buprofezin" into the chemical name search box and click the green
button.
4. Select the 'All Effects' tab from the left frame and scroll down to 'Reproduction
Group'
5. Deselect the checkbox next to 'Any Measurements' and/or just select the
checkbox next to 'Reproduction Group' then
6. Select the 'Aquatic' button for aquatic data or 'Terrestrial' button for terrestrial
data. The data fields and display format are different for an aquatic exposure
versus a terrestrial exposure. If you would like to view data for both Aquatic and
Terrestrial, you can toggle between the two by select either 'Aquatic' gQjfl or
'Terrestrial' ¦HIMIiiH.
7. Click the blue 'View All Applied' button to view/export search parameters applied.
View All Applied
8. Select green "Customize Output Fields" to select additional data fields to be
Customize Output Fields
shown in the output table.
9. Select 'References' tab in upper right to see list of references for the results from
this search.
10. Click the green 'Export as...' drop-down to select type of file to download.
0 Export as...
Note: Please confirm that what you select/deselect makes sense in the database. If
you have selected "Reproduction" and "Growth" you should NOT be seeing any other
Effects in your report. Also, if you are viewing Aquatic Tab in the Results table, you
should not be able to see any Amphibian species under the Results table.
Conversely if you are viewing the Terrestrial table, there should only be TERR
organisms listed, but you can also go back to "All Species" and select smaller group
of results, such as WORMS.
If you have any questions or comments about conducting searches for data in
ECOTOX, please email Ecotox.support@epa.gov or use the "Contact Us' link on
the home page.
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The second example is using the 'Explore' function tab on the homepage. You
might use this when you are not sure of what type of chemical information or
species are in contained in the Knowledgebase.
Explore Example
You want to Explore data on the Reproductive effects of Cadmium on fish.
1. From the ECOTOX home page, click 'Explore' on the top banner.
2. Click the 'Chemicals' icon.
3. Under the Chemicals Groups, scroll down and select Cadmium checkbox.
f 1 Berylli um
Cadmium
Chromium
Explore Data >
4. Click the green 'Explore Data' button to begin exploring.
5. You should see the compounds listed by CAS number. You can browse this list by
CAS, or Chemical name, or number of publications just by typing in the column
header. To Remove this filter, just click on the "X Reset" in upper RED BAR.
6. To view information on specific CAS number listed in output table, click the green
'>' (greater than) sign on the right side of the output table, and all the Aquatic and
Terrestrial Records for that compound will be displayed. You can filter additionally
by Aquatic or Terrestrial by deselecting one or the other. Note: Depending on
number of Records, the output may be limited to the first 3,000.
7. If you want to go back to your original list of compounds, you must go back to
'Query Filters' on left side of the web page, and reset to "All Chemicals" by
selecting then clicking Enter or clicking outside of the box. Using the browser
Back key or closing out the tab above, will bring you all the way back to the
Home page. You can then Select Group summary HitiiiiifiiilHIiiiifl to see original
list
8. From the list of 'Query filters', click the drop down 'Species Group' selection box
and select Fish. If you wish to select multiple species groups, hold the 'ctrl' key
and click on multiple selections. Click outside of the 'Species Group' drop down
menu or click Enter on your keyboard to apply new parameters.
Notice now that above 'Query Filters', the Terrestrial box is no longer highlighted due to
Q AQUATIC TERHESTRI-
the selection of 'Fish' (Aquatic organism) as your species of interest.
You can continue to refine the output by Effect:
9. From the drop down 'Effect Groups' selection box. Scroll down to Select
'Reproduction'. Again, click outside of the dropdown menu or click Enter on your
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keyboard to apply new parameters. On the top left corner of the page, above
additional 'Query Filters', ensure that only the Aquatic checkbox is selected.
10. The data matching your Explore criteria will automatically display in the Group
Summary view. You can switch to view records individually by clicking the
GroupSummary
'Records' button located under the top banner.
11. You can again filter or refine your Table view by typing in a filter in the header (e.g.
"LOEC" under Endpoint) which will reduce the number of Records (red bar). Note:
If you export to CSV file from here, you will only get this subset of records, not all
original record output.
12. To use 'Explore' with the visualization feature, select the 'Plot View' button.
GroupSummary Records
13. There are 3 interactive figures to display ECOTOX Records which have exposure
concentrations (standardized to an equivalent of ppm (parts per million)).
a) Dur x Chem: Exposure duration (days) on x-axis, Exposure concentration on y-
axis, Chemical for point shape and color
b) Dur x Endpt: Exposure duration (days) on x-axis, Exposure concentration on y-
axis, Endpoint for point shape and color
c) Effect x Chem: Effect Groups on x-axis, Exposure concentration on y-axis,
Chemical for point shape and color
Note: You can zoom into data by drawing rectangle around area of interest. Chemicals or
endpoints can be deselected/removed from plot by clicking on name in legend. Hover
over points of interest for more information. Clicking on point in plot will direct you to
corresponding record in output table.
14. Simplified table below each figure shows subset of data for each record.
15. You can continue to refine the output with the query filters on left hand side.
16. Click the blue 'View All Applied' button to view/export Explore parameters applied.
View All Applied
Click the green " Export" button to download figure or simple table,
Note: To see all available data for specific results, users can send the query filters
and search parameters to the 'Search' function of the Knowledgebase using the
green button on the upper right of the Records Table.
Q Send Query Filters to Search O
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APPENDIX C: ECOTOX KNOWLEDGEBASE OVERVIEW
Data Sources
The primary source of toxicity effect information in ECOTOX is from peer reviewed
literature. Pertinent literature is identified through online computerized searches of the
international literature. The computerized searches were initiated with the 1970
publication year and continue through to the present. Comprehensive searches are
designed to include the effect of nearly all toxic substances on aquatic and terrestrial
organisms within the scope of each ECOTOX Knowledgebase systems' guidelines.
Commercial literature sources are continually evaluated for relevance to the ECOTOX
literature searches. The search strategy is evaluated regarding the success ratio of
each search. Additional literature sources include abstract journals, review
bibliographies, and the EPA's Duluth library collection.
The abstracts obtained through computerized searches of abstracting databases are
screened to identify toxicity references applicable to aquatic and terrestrial habitats.
Those references pertinent to one or more of the databases are acquired through a
variety of literature acquisition procedures such as author reprint requests, inter-library
loans, and commercial sources. As the publications are received, a reference number
is assigned for storage and retrieval purposes, and a final check for applicability and
duplication is made. A bibliographic sub-file stores the citations and a reprint of each
publication is archived.
Publications used in ECOTOX usually contain unique data. For various reasons authors
may report the same data point in different publications. If the authors themselves
cross-reference the data, ECOTOX codes the data only once and notes the cross-
reference as part of the bibliographic citation. This type of cross-reference most
frequently occurs in the publication of a thesis and subsequent journal articles. If the
author does NOT acknowledge multiple publications of a single data point, it is likely this
data point will occur in ECOTOX as multiple records, each with a different citation. This
type of publication occurs most frequently when data is published in different sources
such as a textbook and journal article, an agency publication and a journal article, or a
regional journal and an international journal.
Toxicity test data are included unless the data have been cited as published elsewhere.
Data reported in review papers are abstracted from the original publication.
International publications may be reviewed by ECOTOX staff if either an English
abstract or a translated table of data is included. International cooperative efforts with
the Organization for Economic Cooperation and Development (OECD) and Russia
(Borok Institute) have been used to enhance review of the international literature.
Data obtained from independently compiled data files must meet the minimum data
requirements and quality assurance guidelines defined for each ECOTOX
Knowledgebase component. The key data fields that must be included are test
chemical name, test organism, test duration, effect, and effect concentration or
application rate. Documentation describing the test methods must be provided within
the publication. If tests are missing key parameters, the data are rejected. No effort is
made to locate unreported data (e.g., authors are not contacted, citations referring to
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methods used are not obtained). During the incorporation of an electronic data file, a
quality assurance check of the CAS number, species scientific name, and reference
citation is completed. Data files that have been included in the aquatic dataset are the
MED fathead minnow acute toxicity database
(http://archive.epa.aov/med/med archive 03/web/html/prods pubs.html Center for Lake Superior
Studies; University of Wisconsin-Superior, 1984, 1985, 1986, 1988, and 1990), and
data sets from France, Germany, the Netherlands and Russia. ECOTOX also includes
the U.S. EPA OPP's Pesticide Ecotoxicity Database for both aquatic and terrestrial
toxicity tests. Appendix F contains additional information and contacts for
independently compiled data files.
Quality Assurance
Quality assurance procedures begin with literature acquisition and cataloging, and
continue through the chemical and species verification, the literature review process,
data entry, and data retrieval. Data from the ECOTOX literature is extracted by trained
document abstractors. An intensive training period, a well-documented manual (U.S.
EPA 2009), and close interaction with the data coordinator help ensure a high level of
accuracy and consistency in the reviewing process. All of the publications are
independently reviewed by two different reviewers. These reviews are compared and
differences (if any) are documented, discussed, and resolved by the data coordinator.
Aquatic Data Elements
Aquatic data includes toxic effect results from exposures of single chemicals to aquatic
organisms. Bioassays not included are water chemistry effects (e.g., pH), complex
effluents or sediment studies that do not report a water concentration and chemical
mixtures. If a publication contains data for a single chemical besides one of the above
categories of toxicants, the paper is retained and only the single chemical data are
used in ECOTOX. Test organisms are limited to those that are exclusively aquatic.
Amphibian and insect data for purely aquatic life stages of the organism are included.
Information and data for terrestrial life stages of these organisms is included in the
terrestrial database. Classes of organisms associated with the aquatic environment
(e.g., birds, mammals, reptiles) are coded in the terrestrial database. Microbial
communities (bacteria and virus) are omitted from the aquatic database. Terrestrial
plants tested in hydroponic or nutrient solutions are coded in the terrestrial database.
The data elements for each test are grouped by chemical, organism, exposure
conditions, and effect endpoint. The test chemical parameters describe the toxicant,
the associated CAS registry number, and the grade, purity and/or composition of the
toxicant. The test organism parameters define the type of organism and the life-stage
being tested. The test conditions identify the test water, test location, exposure type
and duration, control parameters, and basic water chemistry. The effect endpoint
parameters consist of a code to define the lethal, sublethal, or residue endpoint and
the corresponding test chemical concentration.
Aquatic Effect Parameters
A separate entry is done for each effect or endpoint from either a unique experimental
design or within one design scenario for statistically defined effects or endpoints. If no
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statistics are used to distinguish endpoints or effects and experimental designs are
similar, the data may be combined into one data record. Endpoints always require a
discrete line. Effects lacking an author reported endpoint may be combined based on
statistical representation by the author. Food chain effects are documented for
organisms at the first level of exposure.
Toxicity test results are primarily reported for observations taken during the chemical
exposure. However, when results are reported only for the time period after the
exposure, i.e. recovery or post exposure effects, this type of result is noted by using a
in conjunction with the endpoint/effect code, e.g., ~MOR for a post exposure
mortality effect.
Terrestrial Data Elements
Toxicity data includes toxic effect results from exposures of single chemicals to
terrestrial organisms. The terrestrial toxicity data includes individual dose response
values, if reported. Only quantitative data are extracted from the publication, qualitative
data are excluded. Graphical data may be reported as ranges and are reported by
using <, > or ~ operators with the value.
Bioassays not included are contaminated soils, sediment studies and chemical
mixtures. If a publication contains data for a single chemical besides one of the above
categories of toxicants, the paper is retained and only the single chemical data are
used in ECOTOX. Test organisms are limited to those that are exclusively terrestrial.
The data elements for each test are grouped by chemical, organism, exposure
conditions, and effect endpoint. The test chemical parameters describe the toxicant,
the associated CAS registry number, and the grade, purity and/or composition of the
toxicant. The test organism parameters define the type of organism, organism source
and the life-stage being tested. The test conditions identify the test location, exposure
type and duration, control parameters, and basic soil parameters. The effect endpoint
parameters consist of a code to define the lethal, sublethal, or residue endpoint and
the corresponding test chemical concentration.
If the author does not report data for a terrestrial database field, the field will display a
"NR' (not reported).
The terrestrial data identifies sources of alternative data (domestic, laboratory animal
or plant toxicity and bioaccumulation information) when there is a paucity of
information on wildlife species. Animals associated with the aquatic environment that
breathe using lungs (e.g., ducks, whales) are included in the terrestrial database.
Exposures to the aquatic life stages of amphibians and insects are included in the
aquatic database.
Decisions regarding the inclusion of animal terrestrial species are based on published
terrestrial wildlife toxicity standard methods and procedures documentation. The
priority for the animal portion of the database is wildlife avian species, e.g. mallard,
pheasant or bobwhite; mammalian species, e.g., meadow vole, deer mouse or mink;
and beneficial invertebrate species, e.g., earthworm, honeybee, leafcutter bee or alkali
bee. If data for other species including laboratory, domestic or non-beneficial
organisms are reported in a publication, data for all test species are included in the
Knowledgebase.
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Terrestrial plant data includes native, crop, or weed species. Terrestrial plants tested in
hydroponic or nutrient solutions are included; aquatic plant exposures are recorded in
the aquatic portion of the Knowledgebase.
Test Identification
Test identification number is used to designate each unique test design. A unique test
design may be characterized by a new test chemical, test species, test location, or
exposure type. Additionally, there are experimental design parameters that will
influence a test scenario sufficiently to warrant an independent test record. Such
parameters include tests conducted at different test temperatures or conducted during
different seasons.
References
Center for Lake Superior Environmental Studies, University of Wisconsin-Superior;
1984, 1985, 1986, 1988, and 1990. Acute Toxicities of Organic Chemicals to Fathead
Minnows (Pimephales promelas), Vol. 1-5. University of Wisconsin-Superior, Superior,
Wl.
U.S. Environmental Protection Agency. 2009. MED Ecotoxicology Database Standard
Operating Procedures (prepared by Computer Sciences Corporation), Mid-Continent
Ecology Division, Duluth, MN.
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APPENDIX D: SPECIES AND CHEMICAL VERIFICATION
Species Verification
The test organism is identified by the current scientific name as verified in the
taxonomic literature. For each species entry, the verified name, taxonomic kingdom,
nomenclature history, and verification sources are kept on file for documentation
purposes. A species number can be located using the species scientific name or
common name. ECOTOX retains all species name synonyms that are no longer used
for taxonomic classification. These synonyms are identified within the scientific name
file by a trailing 'Historical name' after the scientific name. Searches in ECOTOX can
be done using the species synonym name, however, output will contain the currently
accepted species name.
Taxonomic kingdoms are divided into plant (including fungi and monera) and animal.
Field studies may report results for a target community (e.g. benthic
macroinvertebrates) or for an entire enclosed ecosystem (e.g. system-level primary
productivity or respiration). If a community of organisms was tested, the species
grouping from the publication is reported.
Taxonomic Hierarchy
User can search on various taxonomic levels from Kingdom down to Subspecies. The
taxonomic levels are verified by the ITIS (Integrated Taxonomic Information System,
located at https://www.itis.gov . If the taxonomic levels are not available with ITIS,
other taxonomic sources are used.
For more information, please refer to the Species Verification Standard Operating
Procedure, linked from the ECOTOX Help page under "More Resources."
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Predefined Special Interest Sources
The species of special interest groups were compiled using the following
references:
Standard Test Species References (Updated annually)
1. Amiard-Triquet, C., Amiard, J. C., and Mouneyrac, C. (2015). Aquatic Ecotoxicology -
Advancing Tools for Dealing with Emerging Risks. Kidlington, Oxford: Elsevier/Academic
Press. Print.
2. ASTM International - Environmental Assessment Standards and Risk Management Standards -
Biological Effects and Environmental Fate. Years 2012-2019.
3. EPA, Office of Prevention, Pesticides and Toxic Substances, Harmonized Test Guidelines, Series
850, Ecological Effects Test Guidelines. Years 2012/2016.
4. EPA, Office of Solid Waste and Emergency Response. (1994). ECO Update Catalogue of
Standard Toxicity Tests for Ecological Risk Assessment Publication 9345.0-051. Volume 2
Number 2.
5. Nikinmaa, M. (2014). An Intro to Aquatic Toxicology. Waltham, MA: Elsevier/Academic Press.
Print.
6. OECD (Organization for Economic Cooperation and Development) Test Guidelines. (2016).
OECD Guidelines for the Testing of Chemicals, Section 2: Effects on Biotic Systems. Years
1984-2018.
7. Rand, G. M. (2003). Fundamentals of aquatic toxicology: effects, environmental fate and risk
assessment - Second Edition. Ecological Services, Inc. New York, NY: Taylor and Francis
Group. Print.
U.S. Threatened and Endangered Species Reference (Updated Quarterly)
U.S. Fish and Wildlife Service (http://ecos.fws.gov/tess_public/pub/adHocSpeciesForm.jsp
U.S. Invasive Species References
1. Swearingen, J., C. Bargeron. 2016 Invasive Plant Atlas of the United States. University of
Georgia Center for Invasive Species and Ecosystem Health.
https://www.invasiveplantatlas.org/distribution.cfm (https://www.invasive.org/species.cfm).
2. University of Georgia - Center for Invasive Species and Ecosystem Health -
https://www.invasive.org/south/index.html (https://www.invasive.org/species.cfm).
3. Species Profiles List - https://www.invasivespeciesinfo.gov/species-type
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Chemical Verification
A standardized identification number and name for each chemical recorded in the
database is used for consistency. Chemicals reported in the ECOTOX Knowledgebase
are cataloged by using a Chemical Abstracts Service (CAS) registry number. If a CAS
registry number is not available for the test chemical, toxicity data cannot be included
in ECOTOX. Toxicants not included in ECOTOX are water chemistry effects (e.g., pH),
complex effluents, chemical mixtures and biological toxicants. If the author states that
a soil nutrient is added to maintain test organism growth, the test is included. If the test
includes a series of nutrient doses and a toxicant to produce interactive effects, this is
considered a mixture and excluded.
Retrieval is made by using the CAS number, chemical name or chemical list. The
Collective Index (CI) name is used as the standardized name for storage and retrieval.
A separate index file is available for screening CAS numbers and chemical names
used in ECOTOX. It is important to stress that users refer to the original publication to
obtain additional test chemical information which may affect the context of toxicity
information retrieved from ECOTOX.
For more information, please refer to the Chemical Verification section, linked from the
ECOTOX Help page under "ECOTOX Coding Documentation."
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APPENDIX E: ECOTOX DATA FIELD DESCRIPTIONS
All associated terms for these fields are located in the ECOTOX Term Appendix,
available linked from the ECOTOX Help page.
Data fields are listed for both aquatic and terrestrial. If the field is only available for one
database, this is noted (Aquatic only or Terrestrial only).
Chemical Fields
Chemical Carrier - Solvent used to dissolve toxicant in solution or positive control.
CAS Number - Chemical Abstracts Service (CAS) Number.
Chemical Name - CAS Collective Index Name.
Chemical Grade - Grade of chemical.
Chemical Purity - Percent purity or active ingredient.
Chemical Formulation - Formulation of chemical.
Chemical Comment - Chemical formulation code, trade names, synonyms, isomer
names.
Chemical Radiolabel - The isotope of a test or carrier chemical. For terrestrial results,
this is not viewable in the browser full data record.
Species Fields
Species Number - Unique number assigned by ECOTOX software.
Species Scientific Name - Currently accepted scientific name (genus,species).
Species Common Name - Species ortaxonomic grouping common name(s).
Organism Source -The source from which the test organism was obtained.
Organism Lifestage - Initial test organism lifestage.
Organism Age - Initial age of the test organism.
Organism Gender - The sex of the organism.
Organism Initial Weight - Initial weight of organism.
Organism Final Weight - Weight of the organism at the time of observation
Organism Comment - Initial age, weight, length, developmental stage or cell
concentration of test organism.
Species Group - Predefined taxonomic groups name. For more documentation and list
of groups, see Predefined Taxonomic Groups.
Species Taxonomic Information - Organism classification hierarchy (Kingdom,
Phylum/Division, Subphylum, Superclass, Class, Order, Family, Genus, Species).
Kingdom - Divides all species into two kingdoms (plant or animal). The plant kingdom
includes Monera and Fungi species. A taxonomic group (e.g., aquatic community,
plankton) that has both plant and animal kingdoms into one result are included in both
plant and animal kingdom search. (Search option only. Not an output option)
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Test Condition Fields
Application Frequency
The number of doses applied during the exposure.
Media Type
Aquatic - Freshwater (FW) tests include those 1) conducted in freshwater,
reconstituted water, distilled water, or tap water or 2) the organism habitat is
exclusively freshwater. Saltwater (SW) tests include those 1) conducted in natural or
artificial seawater, brackish water, or estuarine water or 2) the organism habitat is
exclusively saline.
Note: If a salinity value of four parts per thousand is reported, it is considered a
freshwater test.
Terrestrial - Type of exposure media, (e.g., natural or artificial soil, hydroponic, filter
paper). If an aqueous exposure is conducted in pore water from a specific soil, the soil
parameters in the soil characteristics fields are reported (pH, CEC, OM, etc.). See
Appendix L. Exposure Media Terms in the "ECOTOX Term Appendix" found under the
Help section of the website for more information.
Test Location
Aquatic - A natural study (Field N) is an experiment conducted outdoors in a natural
water body or in an artificial water body that has a natural bottom substrate and
established aquatic communities (e.g. phytoplankton, zooplankton and fish). Outdoor
studies conducted in an artificial water body without a natural bottom substrate are
considered artificial studies (Field A). If the water body cannot be determined to be
natural or artificial it is coded as field unknown (Field U). All other studies are
considered laboratory (LAB) tests.
Terrestrial - A natural field study (Field N) is an experiment conducted outdoors in a
natural setting. The test organisms are sampled in the wild, e.g. population counts.
Outdoor studies conducted in a simulated environment are coded as an artificial field
study (Field A). Artificial field studies include organisms isolated from their natural
environment via an enclosure of some type, e.g. cages or fencing. If the publication
does not provide enough information to distinguish between Field A and Field N, then
use the code Field U to indicate that the field test type is unknown. Laboratory tests
(LAB) are conducted indoors under controlled laboratory conditions.
Exposure Duration
Aquatic - Exposure duration is recorded using the units reported in the literature. For a
fluctuating or intermittent dosing experiment, the total exposure time is recorded. For
any post exposure, the duration of the entire study is reported.
Terrestrial - Time period recorded in the data field is the time of actual exposure to the
chemical. It is assumed that the exposure duration is equivalent to the longest
observation time. In some instances, a biological time is used, such as an exposure
time reported as "until hatch", "growing season" or "after the nth egg has been laid".
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For injection, diet, topical and environmental exposures where the actual exposure is
dependent on biological and environmental conditions, the exposure time is recorded
as equivalent to the study time.
Study Duration
In cases where the observation time is the only duration reported, it is assumed that the
exposure duration is equivalent to the study time.
In some instance, a biological time is used, such as an exposure time reported as "until
harvest", "growing season" or "after the nth egg has been laid." The term that best
describes the author's text is used.
For injection, diet, topical and environmental exposures where the actual exposure is
dependent on biological and environmental conditions, the exposure time is recorded as
equivalent to the study time.
Author Reported and Standardized Duration
ECOTOX offers two output options for duration, the duration as the author reports in the
publication or duration that is converted to a standard unit (days).
Exposure Type
Aquatic - Exposures must either be aqueous, through diet, by injection, or in-vitro.
Terrestrial - The mechanism by which the toxicant was applied. Organisms are typically
exposed to toxicants through diet, injection, topical, in-vitro or environmental routes. On
occasion, an exposure may be through multiple routes (e.g., such as topical and oral).
Exposure types are searched by major exposure groups. However, a more specific
exposure type is displayed in your output (e.g., searching on 'Intercutaneous' is found
under the Injection exposure type).
Habitat
For Terrestrial studies, the habitat as noted by the author, either soil or non-soil.
Aquatic studies are all in water (aqua).
Chemical Analysis
Quantitative analysis of water in test chambers or field sites is considered a measured
concentration. Concentrations that are not analyzed in test chambers or field sites are
considered unmeasured (nominal).
Application Frequency
Author reported frequency of dosing application.
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Study Type
Used to identify field simulation studies. Examples of field study types include
exposures conducted in a mesocosm, microcosm or enclosure.
Test Type
Author reported Test Type for the toxicity study (e.g. ACUTE, CHRONIC, ELS (Early
Life Stage), FLC (Full Life Cycle), or Generational).
Test Method
Denotes the test methodology used for the study, e.g. U.S.EPA or OECD guideline.
Control
Control information for the reported effect may be presented in the text, in a graph, or in
table format. ECOTOX does not make assessments whether the controls were
satisfactory or insufficient (e.g., replicates run, death of control organisms), but rather
documents author reported controls.
Number of Doses
The total number of exposure doses, including the control(s), for each independent test
design.
Doses
For all Aquatic reports and Terrestrial browser viewable, the individual doses used in
the study are summarized in the online report.
For Terrestrial delimited or Excel reports, this data field reports that dose at which
the response was measured.
Experimental Design
Reports any additional study information, such as for field tests exposure system
dimensions (e.g. pond or lake depth, cage or enclosure size), type of artificial substrate,
and physical or chemical water chemistry parameters.
For laboratory studies, this field will contain information about media and test
chambers - if one of the purposes of the study is to compare results observed
under differing test conditions (e.g., pH, temp, humic acid, sediment) or if
commercial media types were used.
Exposure Sample Number
Sample number reflects the initial sample size for each exposure dose, i.e., the number
of test organisms per treatment.
Gender
Identifies the initial sex (ML - Male, FM - Female, BH - Both) of the organism for each
exposure level.
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Ionic Fraction
For ionizing substances (e.g., metals, ammonia), the dose is reported as the ion, if the
concentration presented by the authors is reported as based on the ionic form of the
compound (e.g., organotin as Sn). ECOTOX uses standard periodic table symbols.
Dose Statistical Method
The method used to determine the range around the Dose value, if reported by the
author(s). The terms standard deviation (SD), standard error (SE), range (R),
confidence interval (CI), confidence limits (CL) or confidence value (CV) of the dose
value are noted.
Test Result Parameters
Aquatic - A separate result is recorded for each effect or endpoint from either a unique
experimental design or within one design scenario for statistically defined effects or
endpoints. If no statistics are used to distinguish endpoints or effects and experimental
designs are similar, the data may be combined into one data record. Endpoints always
require a discrete line. Emphasis is placed on recording LC50, LD50, EC50 over other
regression analyzed endpoints (e.g., EC20, LC100, LD10) when an author reports both
endpoints. Effects lacking an author reported endpoint may be combined based on
statistical representation by the author. Food chain effects are recorded for organisms
at the first level of exposure.
Toxicity test results are primarily reported for observations taken during the chemical
exposure. However, when results are reported only for the period of time after the
exposure, i.e. recovery or post exposure effects, this type of result is noted by using
a in conjunction with the endpoint/effect term, e.g., ~MOR for a post exposure
mortality effect.
Endpoint
Endpoint information is entered if it is reported by the author. For the purposes of
ECOTOX, an endpoint is defined as the quantification of an observed effect obtained
through statistics or other means of calculation for the express purpose of comparing
equivalent effects (e.g., LC50). Many terrestrial plant tests do not have associated
endpoints. Prior to 1996, data from the terrestrial plant database structure allowed only
results based on percent change from control.
An asterisk (*) denotes the reported endpoint acronym provided was modified to
conform to the standard database acronym terminology. For example, if the author
reported a TLM, the endpoint was coded as an LC50* The author reported acronym
should appear in EE Remark field.
Effect
Effect information must be provided by the author in order for the test to be included.
For the ECOTOX Knowledgebase purposes, an effect is defined as the observation of
a response resulting from the action of a chemical stressor (e.g., mortality). The listing
of effect measurements can be found by using the ECOTOX Term Appendix found on
the Home page of the website.
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ECOTOX internally categorizes all observed effects under at least one of ten major
effect group codes:
Accumulation (ACC) - Process by which chemicals are taken into and stored in the
organism. Includes lethal body burden.
Behavior (BEH) - Activity of an organism represented by three subgroups, avoidance
(AVO), general behavior (BEH) and feeding behavior (FDB). All effects related to
reproductive behavior are listed under the Reproduction effect group.
Biochemistry (BCM) - Biotransformation or metabolism of chemical compounds, modes
of toxic action, and biochemical organism responses. Biochemical has three
subgroups, biochemical (BCM), enzyme (ENZ) and hormone (HRM) effects.
Cellular (CEL) - Changes in structure and chemical composition of cells and tissues in
organisms. Three cellular subgroups include cellular (CEL) effects, genetics (GEN)
and histology (HIS).
Growth (GRO) - Encompasses individual organism weight, length, development and
morphology. Development (DVP) covers effects on tissue organization in growing early
life stages. Growth (GRO) represents length and weight changes at any point in the life
cycle. Morphology (MPH) measurements and endpoints address the structure (bones)
and form (organ/tissue development) of an organism at any stage of its life history.
Mortality (MOR) - Death of individuals or measurements that indicate death.
Physiology (PHY) - Basic cell and tissue activities. Subgroups include injury (INJ),
immunity (IMM) and intoxication (ITX).
Population (POP) - Effects on species or taxonomic group occupying the same area at
a given time.
Reproduction (REP) - Reproductive behavior, physiology and care of progeny
measurements. Offspring development effects are found in Growth effect group.
Ecosystem (SYS) - Ecosystem processes (PRS) include community structure and
function. Includes microbial processes.
Multiple Effect (MLT) - Change in more than one effect when data were reported as one result.
No Effect (NER) - The author reported an endpoint, but not a specific effect. This term only
exists on a data transferred reference (OPP database (Reference number 344)) and is not a
searchable Effect term.
Trend
The observed or measured response trend as compared to the control is recorded
when textually or graphically reported.
Response Site
A response site or tissue term is used to identify specific body, organ or tissue effect
sites for associated effect measurement.
EEComment
This field contains additional endpoint and/or effect text as described by the author.
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Effect %
Effect is reported as a raw percent value or percent change, e.g., percent of the total
population or percent increase or decrease. The term "COM" is used to denote several
effect measurements or response sites reporting data results as percentages.
Statistical Significance
Statistical analysis as compared to the control(s) in the test result.
Statistical Level
The level of significance (e.g. test statistic) is recorded when the author has reported
statistical analysis in the test result. Terminology for significance level may be presented
as: p =; p< or alpha value. The terminologies are equivalent and are generally in the
range of 0.001 to 0.10.
Bioconcentration
The bioconcentration factor (BCF) or bioaccumulation factor (BAF) is the degree to
which a chemical can be concentrated in the tissues of an organism in the
environment, ,as a result of exposure to chemicals at steady state during the uptake
phase. The BCF/BAF value is equal to the concentration of a chemical in one or more
tissues of the exposed organism, divided by the average exposure concentration of a
chemical in the test. A bioconcentration endpoint is recorded as either wet (or
unknown) or as dry weight (BCF and BCFD, respectively). It is usually reported with
units of L/kg. If a unit cannot be determined from the information in the paper or it is
unitless, RA (ratio) is used for the unit.
Concentration Type
Concentrations based on the active ingredient or formulation, or as the total, un-ionized
or dissolved concentration, are identified.
Endpoint Assignment
Used to identify the source of the effect or endpoint information reported specifically by
the author (P) or assigned by an ECOTOX reviewer (R)). The reviewer only assigns the
endpoint if the author has provided the statistical analysis that support the endpoint.
Concentration/Dose
The concentration or dose reflects either the range of concentrations tested, or if there
is an endpoint reported, the concentration associated with that endpoint. The
confidence interval or range is recorded when available. An asterisk (*) denotes the
concentration has been recalculated from the author's original units to the standard
ug/L or from the metal compound to the active ionic form.
In certain cases, the water concentration is routinely reported as active form of the test
chemical. For metal salts, the concentration is generally expressed as ug ion/L (e.g.,
HgCI is expressed as Hg+). Since 1998, the data distinguish between the metal
compound and the metal ion in the Ion field. Data extracted prior to this date may have
comments regarding ionic fraction in Comment field.
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When an exponential number is reported (e.g., 1 x 106), it is recorded as E+n or E-n
(e.g., 1 E+6).
Note: ECOTOX offers two output options for concentrations in Aquatic studies; the concentration
as the author reports in the publication or the concentration that is converted to a standard unit
(mg/l), if possible.
Result Sample Number
The sample number reflects the sample size (e.g., 10 embryos) that the observation or
response value is based on at each exposure level. Sample units correspond to the
sample number; i.e., the unit on which the measurement or endpoint is based.
For generational studies and measurements based on the progeny, F1, F2, etc. are
noted in the sample unit field.
Observed Duration
This is recorded as the exposure duration when the result value was observed. This
may be plus or minus, any up to the time at which the response to the toxicant was
observed. If the observation time is not reported or unable to be explicitly determined, a
less than or equal to (<=) the exposure duration is displayed.
Observations during the pretreatment time are reported as negative values and
reported as x' for any pretreatment response observations for which time is unknown.
Author Reported and Standardized Duration
ECOTOX offers two output options for duration: the duration as the author reports in the
publication or duration that is converted to a standard unit (days).
Observed Response (Dose Response only)
Response values may include greater than (>), less than (<), minus (-) or approximation
(~) symbols, if used by the author(s). Response values must be numeric and from text or
graph.
Result Statistical Method (Dose Response only)
When the measurement unit includes a standard deviation (SD), standard error (SE),
range (R), confidence interval (CI), confidence limits (CL) or confidence value (CV) of
the response value are noted.
Result % Dry/Wet Weight ()
If the effect measurement is based on dry (D) or wet (W) weight basis, it is denoted.
The percent moisture is reported, the percentage value is also recorded (e.g.
W75%).
Result Percent Lipid ()
Percent lipid in the whole organism or response site as reported by the author.
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Other Effects
Comments regarding other toxicity tests or effects reported in the publication that do
not meet ECOTOX minimum data requirements are recorded in this field. Commas
separate each distinct term and the text ends with a double slash (II).
General Comment
This field contains additional information about any term or data result that does not fit
in the space provided. A complete list of comment identifiers that link to the associated
field is documented in Appendix Gl: Comment Field Header Names.
Water Chemistry Fields (Aquatic only)
These measured values pertain either to the test water chemistry (preferred) or the
dilution water chemistry values. If it is necessary to report the dilution water chemistry,
this is denoted by an asterisk (*). All are expressed as reported by author. For few
specific measurements, see ECOTOX Term Appendix for more information.
Outdoor Test Fields
Habitat Code
The aquatic field tests include the Cowardin* system level classification to describe
major aquatic systems.
*Cowardin, L.M., V.Carter, F.C.Golet and E.T.LaRoe. 1979. Classification of Wetlands and Deepwater
Habitats of the United States. U.S. Fish and Wildlife Service, FWS/OBS-79, 31 p. (
https://www.tws.aov/wetlands/documents/Classification-of-Wetlands-and-Deepwater-Habitats-of-the-
United-States.pdf)
Habitat Comment
The author's description of the water body, (e.g. brackish marsh, wooded swamp)
Substrate Code and Comment
The bottom substrate is recorded using standard substrate definitions.
Water Depth
Water depth of the experimental system.
Geographic Code
The standardized name, based on FIPS (Federal Information Processing Standards)
code, of the country, or United States and Canadian state/province where the test was
performed is displayed. https://en.wikipedia.org/wiki/List of FIPS country codes
Geographic Location
Contains general text about the test site specific geographic identifiers (e.g., lake, river,
bay, field station or city) where the study was performed.
Application Type - The method of application of the chemical in a field study.
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Application Rate
This field contains the application rate value and the units. If an exposure
concentration is not reported, the application rate will be reported. Application rate
units may be recalculated only if the denominator is not equal to one (e.g. 5 g/2.5 ac).
Chemical Half-Life - The test chemical half-life in the system.
Application Date/Season
The application date is recorded for the time of initial exposure. This field includes the
actual date, a partial date or a season. If one pond is exposed multiple times, only the
first application date is reported. If the calendar year date is not reported, but a season
is, the season (Northern Hemisphere) of initial application of the chemical is reported.
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Terrestrial Data Elements
Test Record
A test record number is used to designate each unique test design. A unique test
design may be characterized by a new test chemical, test species, test location, or
exposure type. Additionally, experimental design parameters may influence a test
scenario sufficiently to warrant an independent test record. Such parameters include
tests conducted at different test temperatures or conducted during different seasons.
Test Number
A computer-generated number that designates each unique test design. There can be
many tests number for each reference number.
Exposure Number (Delimited format only)
A sequential number and dose type that identifies each experimental control or dose
level. Control values are given the lowest numeric values, the dose values are added
sequentially from lowest to highest doses.
Example:
1C = Exposure 1 was the control value
2D = Exposure 2 is the lowest dose value
3D = Exposure 3 is the middle dose value
4D = Exposure 4 is the highest dose value
5R = The exposure values are ranged (low - high)
6E = Only endpoint data are presented in the results.
The exposure dose(s) in the experimental methods are recorded, even if the author did
not report an effect result for every dose.
Parent Record and Result Number (Delimited format only)
The hierarchical database contains two fields that link records to the test record (i.e.,
parent file). A computer-generated number that uniquely identifies each result record;
these numbers are used to link between relational data files within a test number. The
Parent Record Number within the Exposure and Result files is the number that links to
the Test file. There may be many result records numbers for each test number.
Test Comment
Additional information related to methodology or techniques used in the experimental
design. Appendix G contains header terms that are used to link the comment to a
specific ECOTOX field.
Exposure Comment
Additional information related to dosing methodology or techniques used in the test.
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Result Comment
Any additional information related to the endpoint or effect response. Appendix G
contains header terms used to link the comment to a specific ECOTOX field.
Soil Parameter Fields (Terrestrial only)
Temperature - Expressed as reported by author.
Soil Type
The classification name of the natural soil or commercial name of the artificial soil used
in the study. If the classification name is not included, the type of soil is recorded using
the author's terminology, e.g., forest soil, sandy loam soil, arboreal coniferous soil.
Soil Sand %, Soil Silt %, Soil Clay %
The soil texture is stated using percentages of sand, silt and/or clay. Bentonite,
kaolinite or montmorillonite etc., are reported as clay.
Soil pH
The pH of the test media is reported. If the pH of the treated media is not presented,
but the pH value is stated for the untreated or acclimation media, an asterisk (*) is
denoted. If the pH of a specific soil type is not given in the publication, a search is
made of the USDA/NRCS National Cooperative Soil Survey (USA) web site
(http://soils.usda.gov/).
Media Organic Matter
If organic matter is reported for the untreated or acclimation media, it will display with
an asterisk (*). If the organic matter of a specific soil type is not provided in the
publication, information from the USDA/NRCS National Cooperative Soil Survey (USA)
is used for the specific soil series.
Media Moisture
The percentage of moisture in the test media is reported. If moisture is reported for the
untreated or acclimation media, this moisture percentage is recorded and denoted with
an asterisk (*).
Media Cation Exchange Capacity
The media cation exchange capacity is reported. If the cation exchange capacity is
reported for the untreated or acclimation media, this value is denoted with an asterisk.
Soil Dose Measured
The toxicant concentration that was measured in the soil. However, the exposure
dose value may or may not reflect the measured values. The Chemical Analysis field
will denote if the exposure dose value is based on the measured values.
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Media Measurement (wet/dry)
Denotes whether the soil concentration was reported based on dry or wet weight basis.
APPENDIX F: INDEPENDENTLY COMPILED DATA FILES
Some independently compiled data sets have been transferred into ECOTOX from
external sources. The data sets must meet the ECOTOX data parameter and quality
assurance guidelines.
The U.S. EPA Duluth data set includes the Acute Toxicity of Organic Chemicals file
which contains data for a single test species (30-day fathead minnow). The U.S. EPA
Office of Toxic Substances is acknowledged for long-term support in the generation of
all acute toxicity data for organic chemicals and contains all test results, including data
not available on-line. These data have been compiled in five volumes titled: Acute
Toxicities of Organic Chemicals to Fathead Minnows (Pimephales promelas), available
from the Center for Lake Superior Environmental Studies, University of Wisconsin,
Superior, Wl.
International cooperative efforts continue with the Organization for Economic
Cooperation and Development (OECD) and other entities to enhance the review and
incorporation of other International literature. Data files from France, Germany, the
Netherlands and Russia have been incorporated into the ECOTOX Knowledgebase.
The Office of Pesticide Program's Pesticide Ecotoxicity Database is a compilation of
the toxic effects data for registered pesticides. These data have been reviewed and
categorized as acceptable for fulfillment of pesticide registration and re-registration
guideline requirements as explained under FIFRA Subdivision E, Parts 158.145 and
158.150. Data for the Pesticide Ecotoxicity Database are drawn from several sources.
The major portion of the data is derived from actual Agency reviews of toxicological
studies conducted by commercial laboratories and submitted by pesticide companies
in support of their products. The U.S. EPA conducts audits of these laboratories on a
periodic basis through the U.S. EPA Office of Compliance and Monitoring. A second
major source of data entries is the numerous studies conducted by U.S. EPA, USDA,
and U.S. FWS laboratories over many years. A third, less utilized source is published
data considered to meet our guideline criteria for acceptable data.
The U.S. Geological Survey, Biological Resources Division, Columbia Environmental
Research Center (CERC) located in Columbia, Missouri
(https://www.usgs.gov/centers/cerc) database summarizes the results from aquatic
acute toxicity tests conducted by the research facility. The acute toxicity test provides a
relative starting point for hazard assessment of contaminants and is required for
federal chemical registration programs such as the Federal Insecticide Fungicide
Rodenticide Act (PL 80-104) as amended by the Federal Environmental Pesticide
Control Act of 1972 (7 U.S.C. 136-136y) and the Toxic Substances Control Act of 1976
(PL 94-469).
Book".
The available data sets, data, institution address and contacts are listed below:
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Institution Contact Information
Data Summary and
Reference Numbers
EPA: Fathead Minnow Acute Toxicity Database (MED)
To obtain hard copies of the University of Wisconsin-Superior (UWS)
volumes contact:
University of Wisconsin/ Lake Superior Research Institute
PO Box 2000
Superior, Wl 54880
Contact: MattTenEyck
E-mail: MTenEyck@uwsuper.edu
Phone: 715-394-8160
For technical information on the database contact:
U.S. EPA/ORD/NHEERL/MED
Contact: Colleen Elonen
E-mail: elonen.colleen@eDa.aov
5 references (#3217,
12447, 12448, 12858,
12859);
730 aquatic records
French (OECD-IRCHA)
Ecotoxicology Department, INERIS
Rue Lavoisier, B.P. 1
F-91710 Vert Le Petit
France
htto://www.ineris.fr/
Contact: Dr. Roger Cabridenc
Phone: 33-1-45960956; Fax: 33-1-45960957
13 references (#20, 3397,
3516, 3517, 3518, 3519,
3520,3521, 5161, 6771,
9170, 10724, 15300);
256 aquatic records
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ECOTOX 5.3 User Guide
Institution Contact Information
Data Summary and
Reference Numbers
German (OECD)
Umweltbundesamt, Federal Environmental Agency
Dienstgebaude Berlin-Mitte
Mauerstrabe 45-52
0-1080 Berlin Germany
Worlitzer Platz 1
06844 Dessau-RoGlau
Germany
Telephone: +49-340-2103-0
Fax: +49-340-2103-2285
Email: Gerlinde.knetsch@uba.de
httos://www. umweltbundesamt.de/sites/default/files/medien/479/dokumente/fl
282 references (citation
refers to OECDG
Database);
8218 aquatic records
941 terrestrial records
ver bibliothek service etc oktober 2018- enalisch.odf
or:
Dieter Schudoma
German Environment Agency
Section IV 2.4: Water-hazardous substances/ Ecotoxicology laboratory
Schichauweg 58,
12307 Berlin
Tel: (+49-30) 8903 4225
Fax: (+49-30) 8903 4233
Email: dieter.schudoma(S)uba.de
Dutch (OECD)
National Institute of Public Health and Environmental Protection
(RIVM/ACT)
PO Box 1, 3720 BA Bilthoven
The Netherlands
httD://rivm.nl/en/
Contact: Dr. Hans Canton
E-mail: ecocr@sb615.rivm.nl
17 references (#5180,
5331,5333, 5336, 5337,
5356,5367, 5370, 5374,
5375,5378, 5390, 5400,
5411,5414, 11039,
11044);
1990 aquatic records
Russia
Borok Institute, Institute for Biology of Inland Waters, Academy of Sciences
152742 Borok, Nekouz, Yaroslavsky Region
Russian Republic
htto://www.ibiw.ru/
Contact: Victor Komov
E-mail: vko mov(®ibiw.varoslavl. ru
55 references
255 aquatic records
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Institution Contact Information
Data Summary and
Reference Numbers
EPA: Office of Pesticides Program Database (OPP)
(Pesticide Ecotoxicity Database -formerly Ecological Effects Database)
U.S. Environmental Protection Agency
Office of Pesticide Programs
Environmental Fate and Effects Division,
Ecological Effects Branch
401 M St. SW
Washington, DC 20460
httDs://www.eDa.aov/Desticide-contacts/office-Desticide-Droarams-contacts-
1 reference (#344);
5593 aquatic records,
4377 terrestrial records
division-and-tooic
Contact: Nicholas Mastrota
E-mail: Mastrota. nicholas@eDa.aov
USGS Acute Toxicity Database (Mayer & Ellersieck, 1986 - commonly
referred to as the "Gold Book")
Columbia Environmental Research Center
U.S. Geological Survey
4200 New Haven Road, Columbia, Missouri 65201
Phone: 573-875-5399
(http://www.cerc.usqs.qov/data/acute/acute.html)
Contact: Linda Sappington
E-mail: linda saDDinaton@usas.aov
1 reference (#6797);
8761 aquatic records
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APPENDIX G: COMMENT FIELD HEADER NAMES
Aquatic Comment Abbreviations
Comment headers codes are used to link additional data provided to the primary
database field.
Header Abbreviation
Associated Field Name
ALK
Alkalinity
APTY
Application Type
AP SEAS
Application Season
AP DATE
Application Date
AP RATE
Application Rate
AP FREQ
Application Frequency
BCF
Bioconcentration
CARRIER
Carrier or Solvent
CHAR
Chemical Comment
CL
Chlorine Value and Unit
COMPEP
Companion Endpoint
CONC
Concentration
COND
Conductivity
CONTR
Control
DEPTH
Water Depth
DNUM
Number of Doses
DO
Dissolved Oxygen
DOSES
Individual Concentration Value and Unit
ETIME
Exposure Time and Unit
FO
Chemical Formulation
FW, SW
Freshwater/Saltwater Exposure Media
GRADE
Chemical Grade
HAB
Habitat Description
HALF
Half Life
HARD
Hardness
HMA
Humic Acid Value and Unit
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Header Abbreviation
Associated Field Name
In EE Comment
Endpt (Endpoint)
In EE Comment
Measurement
In EE Comment
Effect
INTAKE
Food Intake Rate and Unit
LAB, FIELD
Location
LAT
Latitude
LD
Percent Lipid
LEVEL
Statistical Level
LIFESTG
Organism Lifestage
LOC
Location
LONG
Longitude
MSMT
Effect Measurement
NA
Sodium Value and Unit
ORG C
Organic Carbon
PH
PH
POT
Potassium Value and Unit
PURITY
Chemical Purity
RADIO
Chemical Radiolabel
SALIN
Salinity
SAMPN
Sample Number and Unit
SEX
Gender
SIGNIF
Significance
SITE
Response Site
S04
Sulfate Value and Unit
SOLVCHAR
Chemical Carrier Comment
SOLVFO
Chemical Carrier Formulation
SOLVGRADE
Chemical Carrier Grade
SOLVPURITY
Chemical Carrier Purity
SOURCE
Organism Source
STST
Steady State
STYPE
Study Type
SUBSTR
Substrate Comment
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Header Abbreviation
Associated Field Name
SULF
Sulfur Value and Unit
TEMP
Temperature
TESTID
Test Number
TIME
Exposure Time
TREND
Effect Trend
TMETH
Test Method
TYPE
Exposure Type
WTAT
Weight at Time of Result
WTINT
Initial Body Weight
Terrestrial Comment Abbreviations
Comment headers codes are used to link additional data provided to the primary
database field.
Header Abbreviations
Associated Field Name
ANALYSIS
Chemical Analysis
AP DATE
Application Date
AP FREQ
Application Frequency
AP RATE
Application Rate
AP SEAS
Application Season
CEC
Media Cation Exchange Capacity
CHAR
Chemical Comments
COMPEP
Companion Endpoint
CONCTYPE
Concentration Type
CONTR
Control
DNUM
Number of Doses
DOSE/ DUNIT
Exposure Dose and Unit,
DOSES
Individual Concentrations Value and Unit
DW
Dry or Wet Weight
EDES
Experimental Design
EFCT
Effect
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Header Abbreviations
Associated Field Name
EFCT%
Effect Percent
EN DPT
Endpoint Assigned
ETIME
Exposure Duration
FO
Chemical Formulation
GEO
Geographic Code
HABCODE
Habitat Code
HABITAT
Habitat
INTAKE
Intake Rate and Unit
ION
Ionic Fraction
LAT
Latitude
LD
Percent Lipid
LIFESTG/ AGE
Lifestage/Age
LOC
Test Location
LONG
Longitude
MEDIA
Media Type
MOIST
Media Moisture
MSMT
Effect Measurement
OCHAR
Organism Comment
OEF
Other Effects
OM
Media Organic Matter
OTIME
Observation Time
PC, CARRIER
Chemical Name, Type
PH
Media pH
RADIO
Chemical Radiolabel
RSITE
Response Site
RVALUE /RUNIT
Observed Response Value/ Unit
SAMPN/ NUNIT
Sample Number and Unit
SEX
Gender
SIGNIF
Statistical Significance
SOIL
Soil Type
SOURCE
Organism Source
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Header Abbreviations
Associated Field Name
STIME
Study Duration
STST
Steady State
STYPE
Study Type
TEMP
Temperature
TEXTURE
Soil Texture
TREND
Effect Trend
TYPE
Exposure Type
TMETH
Test Method
WTAT
Weight at Time of Result
WTINT
Initial Body Weight
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APPENDIX H: DELIMITED OUTPUT HEADER NAMES
Aquatic Report Output Codes
A forward slash (/) within a field refers to an associated comment in a separate field.
You may need to view the full publication for proper interpretation.
A/ofe: OP = Operator (>, >=, <. =<. =)
Report Header
Delimited File Header Name
Header Name Definition
Alk. (7 fields)
Alkalinity Mean Op
Alkalinity Mean Operator
Alkalinity Mean
Alkalinity Mean Value
Alkalinity Min Op
Alkalinity Minimum Operator
Alkalinity Min
Alkalinity Minimum Value
Alkalinity Max Op
Alkalinity Maximum Operator
Alkalinity Max
Alkalinity Maximum Value
Alkalinity Units
Alkalinity Units
Appl. Date
Application Date
Application Date
Appl. Freq. (7 fields)
Application Frequency Mean Op
Application Frequency Mean Operator
Application Frequency Mean
Application Frequency Mean Value
Application Frequency Min Op
Application Frequency Minimum Operator
Application Frequency Min
Application Frequency Minimum Value
Application Frequency Max Op
Application Frequency Maximum
Operator
Application Frequency Max
Application Frequency Maximum Value
Application Frequency Units
Application Frequency Units
Appl. Rate (2 fields)
Application Rate
Application Rate
Application Units
Application Units
Appl. Seas.
Application Season
Application Season
Appl. Type
Application Type
Application Type
Author
Author
Author
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Report Header
Delimited File Header Name
Header Name Definition
BCF (1 fields)
BCF1 Value Op
First Bioconcentration Factor Mean
Operator
Note: The Concentration
Type determines the
fraction measured in BCF1
and BCF 2.
BCF1 Value
First Bioconcentration Factor Mean Value
BCF1 Min Op
First Bioconcentration Factor Minimum
Operator
BCF1 Min
First Bioconcentration Factor Minimum
Value
BCF1 Max Op
First Bioconcentration Factor Maximum
Operator
BCF1 Max
First Bioconcentration Factor Maximum
Value
BCF2 Value Op
Second Bioconcentration Factor Mean
Operator
BCF2 Value
Second Bioconcentration Factor Mean
BCF2 Min Op
Second Bioconcentration Factor
Minimum Operator
BCF2 Min
Second Bioconcentration Factor
Minimum Value
BCF2 Max Op
Second Bioconcentration Factor
Maximum Operator
BCF2 Max
Second Bioconcentration Factor
Maximum Value
BCF3 Value Op
Third Bioconcentration Factor Mean
Operator
BCF3 Value
Third Bioconcentration Factor Mean
BCF3 Min Op
Third Bioconcentration Factor Minimum
Operator
BCF3 Min
Third Bioconcentration Factor Minimum
Value
BCF3 Max Op
Third Bioconcentration Factor Maximum
Operator
BCF3 Max
Third Bioconcentration Factor Maximum
Value
Calcium (7 fields)
Calcium Mean Op
Calcium Mean Operator
Calcium Mean
Calcium Mean Value
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Report Header
Delimited File Header Name
Header Name Definition
Calcium Min Op
Calcium Minimum Operator
Calcium Min
Calcium Minimum Value
Calcium Max Op
Calcium Maximum Operator
Calcium Max
Calcium Maximum Value
Calcium Units
Calcium Units
CAS#
CAS Number
Test Chemical Abstract Services Registry
Number
Chem. Anal.
Chemical Analysis
Chemical Carrier
Chemical Carrier
Contains all carriers (up to three) and all
the associated information (Chemical
Name, Chemical Grade, Chemical
Formulation, Chemical Radiolabel,
Chemical Characteristics, Chemical
Purity.
Chem. Comment
Chemical Comment
Test Chemical Comment
Chem. Form.
Chemical Formulation
Chemical Formulation
Chem. Grade
Chemical Grade
Chemical Grade
Chem. Half Life (7 fields)
Chemical Half Life Mean OP
Chemical Half Life Mean Operator
Chemical Half Life Mean
Chemical Half Life Mean
Half Life Min OP
Half Life Minimum Operator
Half Life Min
Half Life Minimum
Half Life MaxOP
Half Life Maximum Operator
Half Life Max
Half Life Maximum
Half Life Unit
Half Life Unit
Chemical
Chemical Name
Chemical Name
Chem. Pur. (6 Fields)
Chemical Purity Mean OP
Chemical Purity Mean Operator
Chemical Purity Mean
Chemical Purity Mean
Chemical Purity Min OP
Chemical Purity Minimum Operator
Chemical Purity Min
Chemical Purity Minimum
Chemical Purity Max OP
Chemical Purity Maximum Operator
Chemical Purity Max
Chemical Purity Maximum
Chem. Radiolabel
Chemical Radiolabel
Chemical Radiolabel
Chlorine
Chlorine Mean OP
Chlorine Mean Operator
Chlorine Mean
Chlorine Mean
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Report Header
Delimited File Header Name
Header Name Definition
Chlorine Min OP
Chlorine Minimum Operator
Chlorine Min
Chlorine Minimum
Chlorine Max OP
Chlorine Maximum Operator
Chlorine Max
Chlorine Max
Chlorine Unit
Chlorine Unit
Cone (48 fields) (Author) or
(ug/L)
Concentrationl Mean Op
First Concentration Mean Operator
(Author) or (ug/L)
Concentrationl Mean
First Concentration Mean Value (Author)
or (ug/L)
Concentrationl Min Op
First Concentration Minimum Operator
(Author) or (ug/L)
Concentrationl Min
First Concentration Minimum Value
(Author) or (ug/L)
Concentrationl Max Op
First Concentration Maximum Operator
(Author) or (ug/L)
Concentrationl Max
First Concentration Maximum Value
(Author) or (ug/L)
Concentration Typel
First Concentration Type (Author) or
(ug/L)
Concentration2 Mean Op
Third Concentration Mean Operator
(Author) or (ug/L)
Concentration2 Mean
Third Concentration Mean Value (Author)
or (ug/L)
Concentration2 Min Op
Third Concentration Minimum (Author) or
(ug/L) Operator
Concentration2 Min
Third Concentration Minimum Value
Concentration2 Max Op
Third Concentration Maximum Operator
(Author) or (ug/L)
Concentration2 Max
Third Concentration Maximum Value
(Author) or (ug/L)
Concentration Type 2
Third Concentration Type (Author) or
(ug/L)
Concentrations Mean Op
Third Concentration Mean Operator
(Author) or (ug/L)
Concentrations Mean
Third Concentration Mean Value (Author)
or (ug/L)
Concentrations Min Op
Third Concentration Minimum (Author) or
(ug/L) Operator
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Report Header
Delimited File Header Name
Header Name Definition
Concentrations Min
Third Concentration Minimum Value
Concentrations Max Op
Third Concentration Maximum Operator
(Author) or (ug/L)
Concentrations Max
Third Concentration Maximum Value
(Author) or (ug/L)
Concentration Type 3
Third Concentration Type (Author) or
(ug/L)
Concentration Units
Author or ug/L
Control
Control
Control
Conductiv. (7 fields)
Conductivity Mean Op
Conductivity Mean Operator
Conductivity Mean
Conductivity Mean Value
Conductivity Min Op
Conductivity Minimum Operator
Conductivity Min
Conductivity Minimum Value
Conductivity Max Op
Conductivity Maximum Operator
Conductivity Max
Conductivity Maximum Value
Conductivity Units
Conductivity Units
D.O. (7 fields)
Dissolved Oxygen Mean Op
Dissolved Oxygen Mean Operator
Dissolved Oxygen Mean
Dissolved Oxygen Mean Value
Dissolved Oxygen Min Op
Dissolved Oxygen Minimum Operator
Dissolved Oxygen Min
Dissolved Oxygen Minimum Value
Dissolved Oxygen Max Op
Dissolved Oxygen Maximum Operator
Dissolved Oxygen Max
Dissolved Oxygen Maximum Value
Dissolved Oxygen Units
Dissolved Oxygen Units
Dissolved Inorganic
Carbon (7 fields)
Dissolved Inorganic Carbon
Mean Op
Dissolved Inorganic Carbon Mean
Operator
Dissolved Inorganic Carbon
Mean
Dissolved Inorganic Carbon Mean Value
Dissolved Inorganic Carbon
Min Op
Dissolved Inorganic Carbon Minimum
Operator
Dissolved Inorganic Carbon
Min
Dissolved Inorganic Carbon Minimum
Value
Dissolved Inorganic Carbon
Max Op
Dissolved Inorganic Carbon Maximum
Operator
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Report Header
Delimited File Header Name
Header Name Definition
Dissolved Inorganic Carbon
Maximum
Dissolved Inorganic Carbon Maximum
Value
Dissolved Inorganic Carbon
Units
Dissolved Inorganic Carbon Units
Effect
Effect
Effect
Effect Meas.
Effect Measurement
Effect Measurement
Eff. % (6 fields)
Effect Percent Mean Op
Effect Percent Mean Value Operator
Effect Percent Mean
Effect Percent Mean Value
Effect Percent Min Op
Effect Percent Minimum Value Operator
Effect Percent Min
Effect Percent Minimum Value
Effect Percent Max Op
Effect Percent Maximum Value Operator
Effect Percent Max
Effect Percent Maximum Value
Endpoint
Endpoint
Endpoint
Endpoint Assign.
Endpoint Assignment
Endpoint Assignment
EE Comment
EE Comment
Effect Endpoint (EE) Comment
Exp. Design
Experimental Design
Experimental Design Comment
Exp. Dur. (14 fields)
(Author) or (Days)
Exposure Duration Op
Exposure Duration Operator
Exposure Duration
Exposure Duration Value
Exposure Duration Min Op
Exposure Duration Minimum Operator
Exposure Duration Min
Exposure Duration Minimum Value
Exposure Duration Max Op
Exposure Duration Maximum Operator
Exposure Duration Max
Exposure Duration Maximum Value
Exposure Duration Units
Exposure Duration Units
Exposure Sample Number
Exposure Sample Number
Exposure Sample Number
Exp. Type
Exposure Type
Exposure Type
General Comments
General Comments
General Comments
Geog. Loc
Geographic Location
Geographic Location
Geog. Code
Geographic Code
Geographic Code
Gender
Gender
Gender
Habitat
Habitat
Habitat Code
Habitat Code
Habitat Code
Habitat Code
Habitat Comment
Habitat Comment
Habitat Comment
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Report Header
Delimited File Header Name
Header Name Definition
Hardness (mg/L)(7 fields)
Hardness Mean Op
Hardness Mean Operator
Hardness Mean
Hardness Mean Value
Hardness Min Op
Hardness Minimum Operator
Hardness Min
Hardness Minimum Value
Hardness Max Op
Hardness Maximum Operator
Hardness Maximum
Hardness Maximum Value
Hardness Units
Hardness Units
Humic Acid (mg/L)(7 fields)
Humic Acid Mean Op
Humic Acid Mean Operator
Humic Acid Mean
Humic Acid Mean Value
Humic Acid Min Op
Humic Acid Minimum Operator
Humic Acid Min
Humic Acid Minimum Value
Humic Acid Max Op
Humic Acid Maximum Operator
Humic Acid Maximum
Humic Acid Maximum Value
Humic Acid Units
Humic Acid Units
Intake Rate (7 Fields)
Intake Rate Mean Op
Intake Rate Mean Operator
Intake Rate Mean
Intake Rate Mean Value
Intake Rate Min Op
Intake Rate Minimum Operator
Intake Rate Min
Intake Rate Minimum Value
Intake Rate Max Op
Intake Rate Maximum Operator
Intake Rate Maximum
Intake Rate Maximum Value
Intake Rate Units
Intake Rate Units
Ion (3 fields)
Ionic Fraction 1
Ionic Fraction 1
Ionic Fraction2
Ionic Fraction2
Ionic Fraction3
Ionic Fraction3
Lat/Long
Latitude/Longitude
Latitude/Longitude
Magnesium (mg/L)(7 fields)
Magnesium Mean Op
Magnesium Mean Operator
Magnesium Mean
Magnesium Mean Value
Magnesium Min Op
Magnesium Minimum Operator
Magnesium Min
Magnesium Minimum Value
Magnesium Max Op
Magnesium Maximum Operator
Magnesium Maximum
Magnesium Maximum Value
Magnesium Units
Magnesium Units
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Report Header
Delimited File Header Name
Header Name Definition
Media Type
Media Type
Media Type
Number of Doses
Number of Doses
Number of Doses
Obs. Dur. (14 fields)
(Author) or (Days)
Observed Duration Op
Observed Duration Operator
Observed Duration
Observed Duration Value
Observed Duration Min Op
Observed Duration Minimum Operator
Observed Duration Min
Observed Duration Minimum Value
Observed Duration Max Op
Observed Duration Maximum Operator
Observed Duration Max
Observed Duration Maximum Value
Observed Duration Units
Observed Duration Units
Org. Carb. Type
Value (8 fields)
Organic Carbon Mean Op
Organic Carbon Mean Operator
Organic Carbon Mean
Organic Carbon Mean Value
Organic Carbon Min Op
Organic Carbon Minimum Operator
Organic Carbon Min
Organic Carbon Minimum Value
Organic Carbon Max Op
Organic Carbon Maximum Operator
Organic Carbon Maximum
Organic Carbon Maximum
Organic Carbon Units
Organic Carbon Units
Organic Carbon Type
Organic Carbon Type
Org. Age (7 fields)
Organism Age Mean Op
Organism Age Mean Operator
Organism Age Mean
Organism Age Mean
Organism Age Min Op
Organism Age Minimum Operator
Organism Age Min
Organism Age Minimum
Organism Age Max Op
Organism Age Maximum Operator
Organism Age Max
Organism Age Maximum
Organism Age Units
Organism Age Units
Org. Comment
Organism Comment
Organism Comment
Species Final Weight
Species Final Weight Mean
Op
Species Final Weight Mean Operator
Species Final Weight Mean
Species Final Weight Mean
Species Final Weight Min Op
Species Final Weight Minimum Operator
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Report Header
Delimited File Header Name
Header Name Definition
Species Final Weight Min
Species Final Weight Minimum
Species Final Weight Max Op
Species Final Weight Maximum Operator
Species Final Weight Max
Species Final Weight Maximum
Species Final Weight Units
Species Final Weight Units
Organism Initial Weight
Organism Initial Weight Mean
Op
Organism Initial Weight Mean Operator
Organism Initial Weight Mean
Organism Initial Weight Mean
Organism Initial Weight Min
Op
Organism Initial Weight Minimum
Operator
Organism Initial Weight Min
Organism Initial Weight Minimum
Organism Initial Weight Max
Op
Organism Initial Weight Maximum
Operator
Organism Initial Weight Max
Organism Initial Weight Max
Organism Initial Weight Units
Organism Initial Weight Units
Org. Lifestg.
Organism Lifestage
Organism Lifestage
Organism Source
Organism Source
Organism Source
Other Effects
Other Effects
Other Effects
pH (6 fields)
pH Mean Op
pH Mean Operator
pH Mean
pH Mean Value
pH Min Op
pH Minimum Operator
pH Min
pH Minimum Value
pH Max Op
pH Maximum Operator
pH Maximum
pH Maximum Value
Potassium (7 Fields)
Potassium Mean Op
Potassium Mean Operator
Potassium Mean
Potassium Mean Value
Potassium Min Op
Potassium Minimum Operator
Potassium Min
Potassium Minimum Value
Potassium Max Op
Potassium Maximum Operator
Potassium Maximum
Potassium Maximum Value
Potassium Unit
Potassium Unit
Pub. Year
Publication Year
Publication Year
Ref#
Reference Number
Reference Number
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Report Header
Delimited File Header Name
Header Name Definition
Reference Citation (4
fields)
Author
Title
Publication Year
Source
Author
Title
Publication Year
Source
Res p. Site
Response Site
Response Site
Result Comment
Result Comment
Result Percent Lipid (6
Fields)
Result Percent Lipid Mean Op
Result Percent Lipid Mean Operator
Result Percent Lipid Mean
Result Percent Lipid Mean Value
Result Percent Lipid Min Op
Result Percent Lipid Minimum Operator
Result Percent Lipid Min
Result Percent Lipid Minimum Value
Result Percent Lipid Max Op
Result Percent Lipid Maximum Operator
Result Percent Lipid Maximum
Result Percent Lipid Maximum Value
Result Number
Result Number
Result Sample Number
Result Sample Number Mean
Op
Result Sample Number Mean Operator
Result Sample Number Mean
Result Sample Number Mean Value
Result Sample Number Min
Op
Result Sample Number Minimum
Operator
Result Sample Number Min
Result Sample Number Minimum Value
Result Sample Number Max
Op
Result Sample Number Maximum
Operator
Result Sample Number
Maximum
Result Sample Number Maximum Value
Result Sample Number Unit
Result Sample Number Unit
Result Percent Dry/Wet
Weight
Result Percent Dry/Wet
Weight Mean Op
Result Percent Dry/Wet Weight Mean
Operator
Result Percent Dry/Wet
Weight Mean
Result Percent Dry/Wet Weight Mean
Value
Result Percent Dry/Wet
Weight Min Op
Result Percent Dry/Wet Weight Minimum
Operator
Result Percent Dry/Wet
Weight Min
Result Percent Dry/Wet Weight Minimum
Value
Result Percent Dry/Wet
Weight Max Op
Result Percent Dry/Wet Weight Maximum
Operator
Result Percent Dry/Wet
Weight Maximum
Result Percent Dry/Wet Weight Maximum
Value
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Report Header
Delimited File Header Name
Header Name Definition
Result Percent Dry/Wet
Weight Unit
Result Percent Dry/Wet Weight Unit
Salin. (7 fields)
Salinity Mean Op
Salinity Mean Operator
Salinity Mean
Salinity Mean Value
Salinity Min Op
Salinity Minimum Operator
Salinity Min
Salinity Minimum Value
Salinity Max Op
Salinity Maximum Operator
Salinity Max
Salinity Maximum Value
Salinity Units
Salinity Units
Seas.
Season
Sig. Level
Significance Level Mean Op
Significance Level Mean Operator
Significance Level Mean
Significance Level Mean Value
Significance Level Min Op
Significance Level Minimum Operator
Significance Level Min
Significance Level Minimum Value
Significance Level Max Op
Significance Level Maximum Operator
Significance Level Max
Significance Level Maximum Value
Sodium
Sodium Mean Op
Sodium Mean Operator
Sodium Mean
Sodium Mean Value
Sodium Min Op
Sodium Minimum Operator
Sodium Min
Sodium Minimum Value
Sodium Max Op
Sodium Maximum Operator
Sodium Max
Sodium Maximum Value
Sodium Unit
Sodium Unit
Spec. Common Name
Species Common Name
Species Common Name
Spec. Sci. Name
Species Scientific Name
Species Scientific Name
Stat. Sign if.
Statistical Significance
Statistical Significance
Species Taxon Info. (8
fields)
Species Kingdom
Species Kingdom
Species Phylum
Species Phylum
Species Subphylum
Species Subphylum
Species Superclass
Species Superclass
Species Class
Species Class
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Report Header
Delimited File Header Name
Header Name Definition
Species Genus Species
Species Genus Species
Species Variety
Species Variety
Spec. #
Species Number
Species Number
Steady State
Steady State
Study Duration. (14 fields)
(Author) or (Days)
Study Duration Op
Study Duration Operator
Study Duration
Study Duration Value
Study Duration Min Op
Study Duration Minimum Operator
Study Duration Min
Study Duration Minimum Value
Study Duration Max Op
Study Duration Maximum Operator
Study Duration Max
Study Duration Maximum Value
Study Duration Units
Study Duration Units
Study Type
Study Type
Study Type
Substr. Code
Substrate Code
Substrate Code
Substr. Comments
Substrate Comments
Substrate Comments
Sulfate
Sulfate Mean Op
Sulfate Mean Operator
Sulfate Mean
Sulfate Mean Value
Sulfate Min Op
Sulfate Minimum Operator
Sulfate Min
Sulfate Minimum Value
Sulfate Max Op
Sulfate Maximum Operator
Sulfate Max
Sulfate Maximum Value
Sulfate Unit
Sulfate Unit
Sulfur
Sulfur Mean Op
Sulfur Mean Operator
Sulfur Mean
Sulfur Mean Value
Sulfur Min Op
Sulfur Minimum Operator
Sulfur Min
Sulfur Minimum Value
Sulfur Max Op
Sulfur Maximum Operator
Sulfur Max
Sulfur Maximum Value
Sulfur Unit
Sulfur Unit
Temp. (7 fields)
Temperature Mean Op
Temperature Mean Operator
Temperature Mean
Temperature Mean Value
Temperature Min Op
Temperature Minimum Operator
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Report Header
Delimited File Header Name
Header Name Definition
Temperature Min
Temperature Minimum Value
Temperature Max Op
Temperature Maximum Operator
Temperature Max
Temperature Maximum Value
Temperature Units
Temperature Units
Test#
Test Number
Test Number
Test Location
Test Location
Test Location
Test Method
Test Method
Test Method
Test Type
Test Type
Test Type
Title
Title
Title of the Publication
Trend
Trend
Trend
Water Depth (7 fields)
Water Depth Mean Op
Water Depth Mean Operator
Water Depth Mean
Water Depth Mean Value
Water Depth Min Op
Water Depth Minimum Operator
Water Depth Min
Water Depth Minimum Value
Water Depth Max Op
Water Depth Maximum Operator
Water Depth Max
Water Depth Maximum Value
Water Depth Units
Water Depth Units
Terrestrial Delimited Report Output Codes
A forward slash (/) within a field refers to an associated comment in a separate field.
You will need to view the full publication for proper interpretation.
Note: OP = operator ( >, >=, <. =<. =)
Report Header
Delimited File Header Name
Delimited Header Definition
Appl. Date
Application Date
Appl. Freq. (7 fields)
Application Frequency Mean
Op
Application Frequency Mean Operator
Application Frequency Mean
Application Frequency Mean Value
Application Frequency Min Op
Application Frequency Minimum
Operator
Application Frequency Min
Application Frequency Minimum Value
Application Frequency Max
Op
Application Frequency Maximum
Operator
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Report Header
Delimited File Header Name
Delimited Header Definition
Application Frequency Max
Application Frequency Maximum Value
Application Frequency Units
Application Frequency Units
Appl. Rate (2 fields)
Application Rate
Application Units
Application Rate
Application Units
Appl. Seas.
Application Season
Application Season
Appl. Type
Application Type
Application Type
Author
Author
BCF/BAF
** BCF/BAF values are
located in "Observed
Response Value" field in
the Excel/Delimited
reports**
BCF1 Value Op
Bioconcentration Factor Mean Operator
BCF1 Value
Bioconcentration Factor Mean Value
BCF1 Min Op
Bioconcentration Factor Minimum
Operator
BCF1 Min
Bioconcentration Factor Minimum Value
BCF1 Max Op
Bioconcentration Factor Maximum
Operator
BCF1 Max
Bioconcentration Factor Maximum Value
CAS#
CAS Number
Test Chemical Abstracts Service
Registry Number
Chemical Carrier
Carrier
Contains all carriers (up to three) and all
the associated information (Chemical
Name, Chemical Grade, Chemical
Formulation, Chemical Radiolabel,
Chemical Characteristics, Chemical
Purity.
Chem. Anal.
Chemical Analysis Method
Chemical Analysis Method
Chem. Comment
Chemical Comment
Test Chemical Comment
Chem. Grade
Chemical Grade
Test Chemical Grade
Chem. Form.
Chemical Formulation
Test Chemical Formulation
Chemical Name
Chemical Name
Test Chemical Name
Chem. Pur.
Chemical Purity
Test Chemical Purity
Chem. Radiolabel
Chemical Radiolabel
Test Chemical Radiolabel
Chem. Half Life (7 fields)
Chemical Half Life Mean OP
Chemical Half Life Mean Operator
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Report Header
Delimited File Header Name
Delimited Header Definition
Chemical Half Life Mean
Chemical Half Life Mean
Chemical Half Life Min Op
Chemical Half Life Minimum Operator
Chemical Half Life Min
Chemical Half Life Minimum
Chemical Half Life Max Op
Chemical Half Life Maximum Operator
Chemical Half Life Max
Chemical Half Life Maximum
Chemical Half Life Unit
Chemical Half Life Unit
Dose (10 fields)
Dose Mean Op
Dose Mean Operator
Dose Mean
Dose Mean Value
Dose Min Op
Dose Minimum Operator
Dose Min
Dose Minimum Value
Dose Max Op
Dose Maximum Operator
Dose Max
Dose Maximum Value
Dose Statistical Method
Dose Statistical Method
Dose Value Op
Dose Statistical Method Operator
Dose Value
Dose Statistical Method Value
Dose Units
Dose Units
Control
Control
Control
Dose #
Dose Number
Dose Number
EE Comment
EE Comment
Effect Endpoint (EE) Comment
Effect
Effect
Effect
Effect Meas.
Effect Measurement
Effect Measurement
Eff. % (6 fields)
Effect Percent Mean Op
Effect Percent Mean Value Operator
Effect Percent Mean
Effect Percent Mean Value
Effect Percent Min Op
Effect Percent Minimum Value Operator
Effect Percent Min
Effect Percent Minimum Value
Effect Percent Max Op
Effect Percent Maximum Value Operator
Effect Percent Max
Effect Percent Maximum Value
Endpoint
Endpoint
Endpoint
Endpoint Assign.
Endpoint Assignment
Endpoint Assignment
Experimental Design
Experimental Design
Experimental Design Comment
Exp. Type
Exposure Type
Exposure Type
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Report Header
Delimited File Header Name
Delimited Header Definition
Exposure Comment
Exposure Comment
Exposure Comment
Exp. Dur (Author or Days)
Exposure Mean Op
Exposure Duration Mean Operator
(Author) or (Days)
Exposure Mean
Exposure Duration Mean Value (Author)
or (Days)
Exposure Min Op
Exposure Duration Minimum Operator
(Author) or (Days)
Exposure Min
Exposure Duration Minimum Value
(Author) or (Days)
Exposure Max Op
Exposure Duration Maximum Operator
(Author) or (Days)
Exposure Max
Exposure Duration Maximum Value
(Author) or (Days)
Exposure Duration Units
(Author) or (Days)
Exp. Sample # (7 fields)
Exposure Sample Number
Mean Op
Exposure Sample Number Mean
Operator (Author) or (Days)
Exposure Sample Number
Mean
Exposure Sample Number Mean Value
(Author) or (Days)
Exposure Sample Number
Min Op
Exposure Sample Number Minimum
Operator (Author) or (Days)
Exposure Sample Number
Min
Exposure Sample Number Minimum
Value (Author) or (Days)
Exposure Sample Number
Max Op
Exposure Sample Number Maximum
Operator (Author) or (Days)
Exposure Sample Number
Max
Exposure Sample Number Maximum
Value (Author) or (Days)
Gender
Gender
Gender
Gen. Comments
General Comments
General Comments
Geog. Loc
Geographic Location
Geographic Location
Geog. Code
Geographic Code
Geographic Code
Habitat
Habitat
Habitat
Habitat Code
Habitat Code
Habitat Code
Habitat Comment
Habitat Comment
Habitat Comment
Intake Rate (7 Fields)
Intake Rate Mean Op
Intake Rate Mean Operator
Intake Rate Mean
Intake Rate Mean Value
Intake Rate Min Op
Intake Rate Minimum Operator
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Report Header
Delimited File Header Name
Delimited Header Definition
Intake Rate Min
Intake Rate Minimum Value
Intake Rate Max Op
Intake Rate Maximum Operator
Intake Rate Maximum
Intake Rate Maximum Value
Intake Rate Units
Intake Rate Units
Ion
Ionic Fraction
Ionic Fraction
Lat/Long
Latitude/Longitude
Latitude/Longitude
Media Meas. Wet/Dry
Media Measurement (wet/
dry)
Media Measurement (wet/ dry)
Media CEC (7 fields)
Media CEC Mean Op
Media Cation Exchange Capacity Mean
Operator
Media CEC Mean
Media Cation Exchange Capacity Mean
Value
Media CEC Min Op
Media Cation Exchange Capacity
Minimum Operator
Media CEC Min
Media Cation Exchange Capacity
Minimum Value
Media CEC Max Op
Media Cation Exchange Capacity
Maximum Operator
Media CEC Max
Media Cation Exchange Capacity
Maximum Value
Media CEC Units
Media Cation Exchange Capacity Units
Media Moist. (6 fields)
Media Moisture Mean Op
Media Moisture Mean Operator
Media Moisture Mean
Media Moisture Mean Value
Media Moisture Min Op
Media Moisture Minimum Operator
Media Moisture Min
Media Moisture Minimum Value
Media Moisture Max Op
Media Moisture Maximum Operator
Media Moisture Max
Media Moisture Maximum Value
Media Orgnc. Mat. (7
fields)
Media Organic Matter Mean
Op
Media Organic Matter Mean Operator
Media Organic Matter Mean
Media Organic Matter Mean Value
Media Organic Matter Min Op
Media Organic Matter Minimum Operator
Media Organic Matter Min
Media Organic Matter Minimum Value
Media Organic Matter Max Op
Media Organic Matter Maximum
Operator
Media Organic Matter Max
Media Organic Matter Maximum Value
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ECOTOX 5.3 User Guide
Report Header
Delimited File Header Name
Delimited Header Definition
Media Organic Matter Units
Media Organic Matter Units
Media Type
Media Type
Media Type
Obs. Dur. (Author), (Days)
Observation Duration Mean
Op
Observation Duration Mean Operator
(Author) or (Days)
Observation Duration Mean
Observation Duration Mean Value
(Author) or (Days)
Observation Duration Min Op
Observation Duration Minimum Operator
(Author) or (Days)
Observation Duration Min
Observation Duration Minimum Value
(Author) or (Days))
Observation Duration Max Op
Observation Duration Maximum (Author)
or (Days)
Observation Duration Max
Observation Duration Maximum Value
(Author) or (Days)
Observation Duration Unit
(Author) or (Days)
Observed Response Min
Observed Response Minimum Value
Observed Response Max
Observed Response Maximum Value
Observed Response Value
Observed Response Statistical Method
Value
Observed Response Mean
Op
Observed Response Mean Operator
Observed Response Min Op
Observed Response Minimum Operator
Observed Response Max Op
Observed Response Maximum Operator
Observed Response Units
Observed Response Units
Org. Comment
Organism Comment
Organism Comment
Org. Age (7 fields)
Organism Age Mean OP
Organism Age Mean Operator
Organism Age Mean
Organism Age Mean
Organism Age Min OP
Organism Age Minimum Operator
Organism Age Min
Organism Age Minimum
Organism Age Max OP
Organism Age Maximum Operator
Organism Age Max
Organism Age Maximum
Organism Age Units
Organism Age Units
Org. Comment
Organism Comment
Organism Comment
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ECOTOX 5.3 User Guide
Report Header
Delimited File Header Name
Delimited Header Definition
Org. Init. Wt.
Organism Initial Weight Mean
OP
Organism Initial Weight Mean Operator
Organism Initial Weight Mean
Organism Initial Weight Mean
Organism Initial Weight Min
OP
Organism Initial Weight Minimum
Operator
Organism Initial Weight Min
Organism Initial Weight Minimum
Organism Initial Weight Max
OP
Organism Initial Weight Maximum
Operator
Organism Initial Weight Max
Organism Initial Weight Maximum
Organism Initial Weight Units
Organism Initial Weight Units
Org. Lifestg.
Organism Lifestage
Organism Lifestage
Org. Source
Organism Source
Organism Source
Other Effects
Other Effects
Other Effects
Ref. #
Reference Number
Reference Number
Reference Citation (2
fields, author, year)
Click on "References" to
obtain full citation
Author
Title
Year
Source
Author
Title
Year
Source
Res p. Site
Response Site
Response Site
Result Comment
Result Comment
Result Comment
Result % Dry/Wet Weight
Result Percent Dry/Wet
Weight
Result Percent Dry/Wet Weight
Result % Lipid
Result Percent Lipid
Result Percent Lipid
Result Rec. Num.
Result Record Number
Result Record Number
Result Statistical Method
(see "Observed Response Value" field to
locate associated value)
Result Sample Number
Result Sample Number
Result Sample Unit
Result Sample Unit
Sig. Level
Significance Level
Significance Level
Soil Dose Meas.
Soil Dose
Soil Does Measured
Soil Clay % (6 fields)
Soil Clay Percent Mean Op
Soil Clay Percent Mean Operator
Soil Clay Percent Mean
Soil Clay Percent Mean Value
Soil Clay Percent Min Op
Soil Clay Percent Minimum Operator
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ECOTOX 5.3 User Guide
Report Header
Delimited File Header Name
Delimited Header Definition
Soil Clay Percent Min
Soil Clay Percent Minimum Value
Soil Clay Percent Max Op
Soil Clay Percent Maximum Operator
Soil Clay Percent Maximum
Soil Clay Percent Maximum Value
Soil Sand % (6 fields)
Soil Sand Percent Mean Op
Soil Sand Percent Mean Operator
Soil Sand Percent Mean
Soil Sand Percent Mean Value
Soil Sand Percent Min Op
Soil Sand Percent Minimum Operator
Soil Sand Percent Min
Soil Sand Percent Minimum Value
Soil Sand Percent Max Op
Soil Sand Percent Maximum Operator
Soil Sand Percent Max
Soil Sand Percent Maximum Value
Soil Silt % (6 fields)
Soil Silt Percent Mean Op
Soil Silt Percent Mean Operator
Soil Silt Percent Mean
Soil Silt Percent Mean Value
Soil Silt Percent Min Op
Soil Silt Percent Minimum Operator
Soil Silt Percent Min
Soil Silt Percent Minimum Value
Soil Silt Percent Max Op
Soil Silt Percent Maximum Operator
Soil Silt Percent Max
Soil Silt Percent Maximum Value
Soil pH (6 fields)
Soil pH Mean Op
Soil pH Mean Operator
Soil pH Mean
Soil pH Mean Value
Soil pH Min Op
Soil pH Minimum Operator
Soil pH Min
Soil pH Minimum Value
Soil pH Max Op
Soil pH Maximum Operator
Soil pH Max
Soil pH Maximum Value
Soil Type
Soil Type
Soil Type
Source
Source
Bibliographic Source
Spec. Common Name
Species Common Name
Org. Final Wt
Species Final Weight Mean
Op
Species Final Weight Mean Operator
Species Final Weight Mean
Species Final Weight Mean
Species Final Weight Min Op
Species Final Weight Minimum Operator
Species Final Weight Min
Species Final Weight Minimum
Species Final Weight Max Op
Species Final Weight Maximum Operator
Species Final Weight Max
Species Final Weight Maximum
Species Final Weight Units
Species Final Weight Units
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ECOTOX 5.3 User Guide
Report Header
Delimited File Header Name
Delimited Header Definition
Species Group
Species Group
Species Group
Species Taxon. Info. (8
Species Kingdom
Species Kingdom
fields)
Species Phylum
Species Phylum
Species Subphylum
Species Subphylum
Species Superclass
Species Superclass
Species Class
Species Class
Species Genus Species
Species Genus Species
Species Variety
Species Variety
Spec. #
Species Number
Species Number
Spec. Sci. Name
Species Scientific Name
Species Scientific Name
Sign if.
Statistical Significance
Statistical Significance
Steady State
Steady State
Steady State
Study Dur. (7 fields)
Study Mean Op
Study Duration Mean Operator (Author)
or (Days)
Study Mean
Study Duration Mean Value (Author) or
(Days)
Study Min Op
Study Duration Minimum Operator
(Author) or (Days)
Study Min
Study Duration Minimum Value (Author)
or (Days)
Study Max Op
Study Duration Maximum Operator
(Author) or (Days)
Study Max
Study Duration Maximum Value (Author)
or (Days)
Study Duration Unit
(Author) or (Days)
Test Comments
Test Comment
Test Comment
Test Loc.
Test Location
Test Location
Test#
Test Number
Test Number
Test Method
Test Method
Test Method
Test Type
Test Type
Test Type
Trend
Trend
Trend
Title
Title
Title of Publication
Pub. Year
Year
Publication Year
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