PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
Risk Evaluation for
T richloroethylene
Systematic Review Supplemental File:
Data Extraction for Environmental Hazard Studies
CASRN: 79-01-6
xvEPA
United States
Environmental Protection Agency
Office of Chemical Safety and
Pollution Prevention
CI H
CI CI
February 2020

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PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
Environmental Hazard Data Extraction Table for Trichloroethylene (TCE)
Test Species
Fresh/
Salt
Water
Duration
End-point
Concentration(s)
Test Analysis
Effect(s)
References
Data Quality
Evaluation
Fish
Bluegill (Lepomis
macrochirus)
Fresh
24-hour
LCso = >68,
<100 mg/L
Not reported
Static, Nominal,
Solvent: unknown
Mortality
(Buccafusco et al..
1981)
Medium
Bluegill (Lepomis
macrochirus)
Fresh
96-hour
LCso = 45 mg/L
Not reported
Static, Nominal,
Solvent: unknown
Mortality
(Buccafusco et al..
1981)
Medium
Fathead minnow
{Pimephales
promelas)
Fresh
96-hour
LCso = 44.1 mg
AI/L
<0.01, 8.43-9.64,
15.3-15.9, 27.1-
27.8, 43.4-44.8,
76.6-77.3 mg/L
Flow-through,
Measured
Mortality
(Geieer et al..
1985)
High
Fathead minnow
(Pimephales
promelas)
Fresh
24-hour
LCso = 52.4 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Mortality
(Alexander et al..
1978)
High
Fathead minnow
(Pimephales
promelas)
Fresh
24-hour
LCio = 34.7 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Mortality
(Alexander et al..
1978)
High
Fathead minnow
(Pimephales
promelas)
Fresh
24-hour
LC90 = 79.1 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Mortality
(Alexander et al..
1978)
High
Fathead minnow
(Pimephales
promelas)
Fresh
48-hour
LCso = 53.3 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Mortality
(Alexander et al..
1978)
High
* Purity was not reported, so value is as reported by authors.

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PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qusili(>
l'\si In sit ion
I'alhead minnow
(Pimephales
promelas)
I'resli
4X-lkUII'
i.( " in-
AI/L*
\nl ivpoi'lcd
I'low -ihroiidi.
Measured, Solvent:
Methyl or ethyl
alcohol
Moilalils
(
1978)
1 huh
Fathead minnow
{Pimephales
promelas)
Fresh
48-hour
LC90 = 102.6
mg AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Mortality
(Alexander et aL
1978)
High
Fathead minnow
{Pimephales
promelas)
Fresh
72-hour
LC50 = 39.0 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Mortality
(Alexander ef aL
High
Fathead minnow
{Pimephales
promelas)
Fresh
72-hour
LC10 = 20.9 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Mortality
(Alexander et aL
High
Fathead minnow
{Pimephales
promelas)
Fresh
72-hour
LC90 = 72.6 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Mortality
(Alexander et aL
High
Fathead minnow
{Pimephales
promelas)
Fresh
96-hour
LC50 = 40.7 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Mortality
(Alexander et aL
High
Fathead minnow
{Pimephales
promelas)
Fresh
96-hour
LC10 = 17.4 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Mortality
(Alexander et aL,
1978)
High
* Purity was not reported, so value is as reported by authors.

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PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qusili(>
l'\si In sit ion
I'alhead minnow
(Pimephales
promelas)
I'resli

l.( (i inu
AI/L*
\nl ivpoi'lcd
I'low -ihroiidi.
Measured, Solvent:
Methyl or ethyl
alcohol
Moilalils
(
1978)
1 huh
Fathead minnow
{Pimephales
promelas)
Fresh
96-hour
LCso = 66.8 mg
AI/L*
Not reported
Static, Nominal,
Solvent: Methyl or
ethyl alcohol
Mortality
(Alexander et aL
1978)
Medium
Fathead minnow
{Pimephales
promelas)
Fresh
24-hour
EC50 = 23 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Loss of
equilibrium
(Alexander ef aL
1978)
High
Fathead minnow
{Pimephales
promelas)
Fresh
24-hour
EC10 = 15.2 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Loss of
equilibrium
(Alexander et aL
High
Fathead minnow
{Pimephales
promelas)
Fresh
24-hour
EC90 = 16.2 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Loss of
equilibrium
(Alexander et aL
High
Fathead minnow
{Pimephales
promelas)
Fresh
48-hour
EC50 = 22.7 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Loss of
equilibrium
(Alexander et aL
High
Fathead minnow
{Pimephales
promelas)
Fresh
48-hour
EC10 = 16.9 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Loss of
equilibrium
(Alexander et aL
1978)
High
* Purity was not reported, so value is as reported by authors.
* Purity was not reported, so value is as reported by authors.

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PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qusili(>
l'\si In sit ion
I'alhcad iiiiiimow
(Pimephales
promelas)
I'resli
4S-Ikmii'
l!( Milium
AI/L*
\nl ivpoi'lcd
I'low -ihroiidi.
Measured, Solvent:
Methyl or ethyl
alcohol
1 .OSS 111
equilibrium
(
1978)
1 huh
Fathead minnow
{Pimephales
promelas)
Fresh
72-hour
ECso = 22.2 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Loss of
equilibrium
(Alexander et aL
1978)
High
Fathead minnow
{Pimephales
promelas)
Fresh
72-hour
EC10 = 15.5 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Loss of
equilibrium
(Alexander ef aL
High
Fathead minnow
{Pimephales
promelas)
Fresh
72-hour
EC90 = 31.3 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Loss of
equilibrium
(Alexander et aL
High
Fathead minnow
{Pimephales
promelas)
Fresh
96-hour
EC50 = 21.9 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Loss of
equilibrium
(Alexander et aL
High
Fathead minnow
{Pimephales
promelas)
Fresh
96-hour
EC10 = 13.7 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Loss of
equilibrium
(Alexander et aL
High
Fathead minnow
{Pimephales
promelas)
Fresh
96-hour
EC90 = 34.9 mg
AI/L*
Not reported
Flow-through,
Measured, Solvent:
Methyl or ethyl
alcohol
Loss of
equilibrium
(Alexander et aL,
1978)
High

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PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qusili(>
l'\si In sit ion
I'alhcad iiiiiiiiow
(Pimephales
promelas)
I'resli
^:-da>
i:c in s inu
AI/L (Test 3)
\nl ivpoi'lcd
I'low -ihroiidi.
Measured
(ii'oulh Wei
weight
i
2005)
1 huh
Fathead minnow
{Pimephales
promelas)
Fresh
32-day
EC20 = 7.59 mg
AI/L (Test 3)
Not reported
Flow-through,
Measured
Growth: Wet
weight
(Broderius et al.„
2005)
High
Fathead minnow
{Pimephales
promelas)
Fresh
96-hour
LC50 = 55.2 mg
AI/L (Test 1)
Not reported
Flow-through,
Measured
Mortality
(Broderius ef al.„
2005)
High
Fathead minnow
{Pimephales
promelas)
Fresh
96-hour
LC50 = 40.5 mg
AI/L (Test 2)
Not reported
Flow-through,
Measured
Mortality
(Broderius et al.„
2005)
High
Fathead minnow
{Pimephales
promelas)
Fresh
96-hour
LC50 = 45.7 mg
AI/L (Test 3)
Not reported
Flow-through,
Measured
Mortality
(Broderius et al.„
2005)
High
Fathead minnow
{Pimephales
promelas)
Fresh
32-day
EC50 = 11.9 mg
AI/L (Test 1)
Not reported
Flow-through,
Measured
Growth: Wet
weight
(Broderius ef al.„
2005)
High
Fathead minnow
{Pimephales
promelas)
Fresh
32-day
EC20 = 7.93 mg
AI/L (Test 1)
Not reported
Flow-through,
Measured
Growth: Wet
weight
(Broderius et al.
2005)
High
Fathead minnow
{Pimephales
promelas)
Fresh
32-day
EC50 = 10.5 mg
AI/L (Test 2)
Not reported
Flow-through,
Measured
Growth: Wet
weight
(Broderius et al.
2005)
High
Fathead minnow
{Pimephales
promelas)
Fresh
32-day
EC20 = 7.71 mg
AI/L (Test 2)
Not reported
Flow-through,
Measured
Growth: Wet
weight
(Broderius et al.
2005)
High
Fathead minnow
{Pimephales
promelas)
Cutlure
medium
24-hour
EC5o= 11,600
mg/L
Not reported
In vitro, Nominal,
Carrier: liquid paraffin
Inhibition of total
protein content
(Dierickx. 1993)
Low
Flagfish
{Jordanella
floridae)
Fresh
96-hour
LC50 = 28.28
mg AI/L
Not reported
Flow-through,
Measured, Solvent:
Acetone
Mortality
(Smith et al. 1991)
High

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PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
l ost Ansilvsis
r.lTecl(s)
Uelerenees
Dsilsi Qusili(>
l'\si In sit ion
I'hiul'ish
(.Jordanella
floridae)
I'resli
i:. :4.
48, 72-
hour
i.( :«M(.
mg AI/L
\nl ivpoi'lcd
I'low -ihroiidi.
Measured, Solvent:
Acetone
Moilalils
( )
1 hull
Flagfish
{Jordanella
floridae)
Fresh
96-hour
LC50 = 3.1 mg/L
Not reported
Renewal, Nominal,
Solvent: Acetone
Mortality
(Smith et al. 1991)
Medium
Flagfish
{Jordanella
floridae)
Fresh
10-day
LOEC >21.2
mg AI/L
0, 1.683,2.301,
4.355,5.758,21.233
mg/L
Flow-through,
Measured, Solvent:
Acetone
Egg hatchability
(Smith et al. 1991)
High
Flagfish
{Jordanella
floridae)
Fresh
10-day
LOEC = 11 mg
AI/L
0, 1.683,2.301,
4.355,5.758,21.233
mg/L
Flow-through,
Measured,
Solvent: Acetone
Larval survival
(Smith et al. 1991)
High
Flagfish
{Jordanella
floridae)
Fresh
28-day
LOEC = 14.85
mg AI/L
0,2.516,3.623,
7.769, 10.568,
20.915 mg/L
Flow-through,
Measured,
Solvent: Acetone
Fry survival
(Smith et al, 1991)
High
Flagfish
{Jordanella
floridae)
Fresh
28-day
LOEC >20.9
mg AI/L
0,2.516,3.623,
7.769, 10.568,
20.915 mg/L
Flow-through,
Measured, Solvent:
Acetone
Fry growth
(Smith et al. 1991)
High
Japanese medaka
{Oryzias latipes)
Fresh
10-day
LC50 = 82 mg
AI/L
54, 59, 65, 72, 79,
87 mg/L
Renewal, Nominal
Mortality
(Scfaell. 1987)
High
Japanese medaka
{Oryzias latipes)
Fresh
10-day
LC100 = 100 mg
AI/L
54, 59, 65, 72, 79,
87 mg/L
Renewal, Nominal
Mortality
(ScfaelL 1987)
High
Japanese medaka
{Oryzias latipes)
Fresh
10-day
NOEC = 40 mg
AI/L
54, 59, 65, 72, 79,
87 mg/L
Renewal, Nominal
Mortality
(Scfaell. 1987)
High
Rose bitterling
{Rhodeus
ocellatus)
Fresh
24-hour
NOAEL = 30
mg AI/L
0,0.03,0.3,3,30
mg/L (exposure
group 1)
Static, Nominal
Micronuclei
(Havashi et al.
1998)
Medium
Rose bitterling
{Rhodeus
ocellatus)
Fresh
24-hour
NOAEL = 30
mg AI/L
0,0.03,0.3,3,30
mg/L (exposure
group 1)
Static, Nominal
Chromosomal
aberrations,
structural
(Havashi et al.
.1.998)
Medium
Rose bitterling
{Rhodeus
ocellatus)
Fresh
24-hour
NOAEL = 30
mg AI/L
0,0.03,0.3,3,30
mg/L (exposure
group 1)
Static, Nominal
Chromosomal
aberrations,
numerical
(Havashi et al..
.1.998)
Medium
Rose bitterling
{Rhodeus
ocellatus)
Fresh
24-hour
NOAEL = 300
mg AI/L
0,0.03,0.3,3,30,
300 mg/L (exposure
group 2)
Static, Nominal
Chromosomal
aberrations,
structural
(Havashi et al.
.1.998)
Medium

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PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uel'erenees
Dsilsi Qusili(>
l'\si In sit ion
Rose hlllci'llliu
(Rhodeus
ocellatus)
I'resli
24-lkuii'
\o\i:i. -no
mg AI/L
0. 0 ill. 0 V V lo.
300 mg/L (exposure
group 2)
Sialic. Nominal
(liioinosonKil
aberrations,
numerical
i
1998)
Medium
Rose bitterling
(Rhodeus
ocellatus)
Fresh
24-hour
NOAEL = 300
mg AI/L
0,0.03, 0.3,3,30,
300 mg/L (exposure
group 2)
Static, Nominal
Micronuclei
(Havashi et al.„
.1.998)
Medium
Rose bitterling
(Rhodeus
ocellatus)
Fresh
24-hour
NOAEL = 300
mg AI/L
LOAEL = 3,000
mg AI/L
0, 300, 3,000 mg/L
(exposure group 3)
Static, Nominal
Chromosomal
aberrations,
structural
(Havashi et al.
.1.998)
Medium
Rose bitterling
(Rhodeus
ocellatus)
Fresh
24-hour
NOAEL = 300
mg AI/L
LOAEL = 3,000
mg AI/L
0, 300, 3,000 mg/L
(exposure group 3)
Static, Nominal
Chromosomal
aberrations,
numerical
(Havashi et al.
.1.998)
Medium
Rose bitterling
(Rhodeus
ocellatus)
Fresh
24-hour
NOAEL = 300
mg AI/L
LOAEL = 3,000
mg AI/L
0, 300, 3,000 mg/L
(exposure group 3)
Static, Nominal
Micronuclei
(Havashi et al.
.1.998)
Medium
Sheepshead
minnow
(Cyprinodon
variegatus)
Salt
96-hour
LC50 = 99 mg
AI/L (based on
initial meas.
conc.)
0, 14, 34, 78, 148,
357 mg/L
Static, Measured
Mortality
(Ward et al. 1.986)
Medium
Sheepshead
minnow
(Cyprinodon
variegatus)
Salt
96-hour
LC50 = 52 mg
AI/L
0, 14, 34, 78, 148,
357 mg/L
Static, Measured
Mortality
(Ward et al. .1.986)
Medium
Sheepshead
minnow
(Cyprinodon
variegatus)
Salt
96-hour
LOAEL = 357
mg AI/L
0, 14, 34, 78, 148,
357 mg/L
Static, Measured
Intoxication
(Ward et al. .1.986)
Medium
Japanese medaka
(Orizias latipes)
Fresh
48-hour
LC50 = 1.9 mg
AI/L
Not reported
Static, Nominal
Mortality
(Yoshioka et al.
.1.986)
Unacceptable
Bluegill (Lepomis
macrochirus)
Fresh
14-day
BCF = 17
0.00823 mg/L
(mean water
concentration)
Flow-through,
Measured
Residue
(Barrows et al.
.1.980)
Unacceptable

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PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uel'erenees
Dsilsi Qusili(>
l'\si In sit ion
1 illlCglll ( /
macrochirus)
I'resli
l4-da\
1 l;ill-l lie in
Tissue < 1 day
	^ mg 1.
(mean water
concertation)
I'low -llii'iiiiuli.
Measured
1 lall'-l.ile of mean
measured
residue
concentration in
tissues
(
1980)
I iiacccpiahlc
Golden orf
(Leuciscus idus)
Fresh
48-hour
LC5o= 120 mg
AI/L
Not reported
Flow-through,
Measured
Mortality
(U mweltbundesa m
Unacceptable
t. 1984)
Zebrafish
(Brachydanio
rerio)
Fresh
14-day
BCF = 12
(initial
concentration)
2.5 mg/L
Semi-static,
Measured
Residue
(U mweltbundesa in
Unacceptable
t 1984)
Zebrafish
(Brachydanio
rerio)
Fresh
14-day
BCF = 65
(concentration
after 48 hours)
2.5 mg/L
Semi-static,
Measured
Residue
(U mweltbundesa m
t. .1.984)
Unacceptable
Zebrafish
(Brachydanio
rerio)
Fresh
14-day
BCF = 19
(concentration
after 48 hours)
2.5 mg/L
Semi-static,
Measured
Residue
(U mweltbundesa m
t. .1.984)
Unacceptable
Aquatic Invertebrates
Water flea
(Daphnia magna)
Fresh
48-hour
ec50 =
7.7519451 mg
AI/L
Not reported
Static, Nominal
Immobilization
(Abernethv et a.L
.1.986)
Medium
Water flea
(Daphnia magna)
Fresh
48-hour
NOEC = 2.2
mg/L
Not reported
Static, Nominal,
Solvent: Unknown
Mortality
(UfflecjiS!)
High
Water flea
(Daphnia magna)
Fresh
48-hour
LC50 =18 mg/L
Not reported
Static, Nominal,
Solvent: Unknown
Mortality
(LeBli"'c-l980>
High
Water flea
(Daphnia magna)
Fresh
24-hour
LC50 = 22 mg/L
Not reported
Static, Nominal,
Solvent: Unknown
Mortality
(U-BIanc, 1980)
High
Water flea
(Daphnia magna)
Fresh
7-day
Minimum
Threshold
Concentration
(MTC) = >10,
<18 mg AI/L
0, 0, 0.62, 1.2, 2.5,
5.0, 10 mg/L
Flow-through,
Nominal, Solvent:
Triethylene glycol
Reproduction:
Number of young
produced per
adult
(Leblanc and
Suiprenant, 1.980)
Unacceptable
Water flea
(Daphnia magna)
Fresh
14-day
MTC = >10,
<18 mg AI/L
0, 0, 0.62, 1.2, 2.5,
5.0, 10 mg/L
Flow-through,
Nominal, Solvent:
Triethylene glycol
Reproduction:
Number of young
produced per
adult
(Leblanc and
Suroreran!, .1.980)
Unacceptable

-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Diirsilinn
Kiul-pninl
(oneenlrsilionis)
Tesl An;il\sis
r.lTecl(s)
Uelerences
Dsilsi Qii;ilil>
l'\si In sit ion
Wilier lien
(Daphnia magna)
I'resli
:i-da>
MTC |u.
<18 mg AI/L
ii. u. u (.2. 1 2. 2 5.
5.0, 10 mg/L
I'lou -lliroiigli.
Nominal, Solvent:
Triethylene glycol
Reproduction
Number of young
produced per
adult
i
Surprenanl 1980)
I naccepiahle
Water flea
(.Daphnia magna)
Fresh
14-day
NOAEL = 10
mg AI/L
0, 0, 0.62, 1.2, 2.5,
5.0, 10 mg/L
Flow-through,
Nominal, Solvent:
Triethylene glycol
Mortality
CLeblanc and
Surprenanl 1980)
Unacceptable
Water flea
(.Daphnia magna)
Fresh
21-day
NO A F.I. = 10
mg AI/L
0, 0, 0.62, 1.2, 2.5,
5.0, 10 mg/L
Flow-through,
Nominal, Solvent:
Triethylene glycol
Mortality
(Leblanc and
Surprenanl 1980)
Unacceptable
Water flea
(.Daphnia magna)
Fresh
14-day
MTC = >10,
<18 mg AI/L
0, 0, 0.62, 1.2, 2.5,
5.0, 10 mg/L
Flow-through,
Nominal, Solvent:
Triethylene glycol
Mortality
(Leblanc and
Surprenanl 1980)
Unacceptable
Water flea
(.Daphnia magna)
Fresh
21-day
MTC = >10,
<18 mg AI/L
0, 0, 0.62, 1.2, 2.5,
5.0, 10 mg/L
Flow-through,
Nominal, Solvent:
Triethylene glycol
Mortality
(Leblanc and
Surprenanl 1980)
Unacceptable
Water flea
(Daphnia magna)
Fresh
7-day
NOAEL = 10
mg AI/L
0, 0, 0.62, 1.2, 2.5,
5.0, 10 mg/L
Flow-through,
Nominal, Solvent:
Triethylene glycol
Mortality
(Leblanc and
Surprenanl 1980)
Unacceptable
Water flea
(.Daphnia magna)
Fresh
7-day
NOAEL = 10
mg AI/L
0, 0, 0.62, 1.2, 2.5,
5.0, 10 mg/L
Flow-through,
Nominal, Solvent:
Triethylene glycol
Reproduction:
Number of young
produced per
adult
(Leblanc and
Surprenanl 1980)
Unacceptable
Water flea
(.Daphnia magna)
Fresh
14-day
NOAEL = 10
mg AI/L
0, 0, 0.62, 1.2, 2.5,
5.0, 10 mg/L
Flow-through,
Nominal, Solvent:
Triethylene glycol
Reproduction:
Number of young
produced per
adult
(Leblanc and
Surprenanl 1980)
Unacceptable
Water flea
(Daphnia magna)
Fresh
21-day
NOAEL = 10
mg AI/L
0, 0, 0.62, 1.2, 2.5,
5.0, 10 mg/L
Flow-through,
Nominal, Solvent:
Triethylene glycol
Reproduction:
Number of young
produced per
adult
(Leblanc and
Surprenanl 1980)
Unacceptable
Water flea
(.Daphnia magna)
Fresh
7-day
MTC = >10,
<18 mg AI/L
0, 0, 0.62, 1.2, 2.5,
5.0, 10 mg/L
Flow-through,
Nominal, Solvent:
Triethylene glycol
Mortality
(Leblanc and
Surprenanl 1980)
Unacceptable
Water flea
(Daphnia magna)
Fresh
24-hour
LC50 = 43.14
mg/L
0, 5, 10, 20, 30, 40,
50, 75, 100 mg/L
Aquatic-not reported,
Nominal
Mortality
(Dobaradaran et
al. 2012)
Medium

-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Diirsilinn
Kiul-pninl
(oneenlrsilionis)
Tesl An;il\sis
r.lTecl(s)
Uelerences
Dsilsi Qu;ilil>
l'\si In sit ion
Wilier lien
I'resli
4X-lkiur
1.( ' ' X5
ii. 5. In. 2d. 'ii. 4(1.
\qiialic-iKi| reported.
Morialiis
i
Medium
(Daphnia magna)


mg/L
50, 75, 100 mg/L
Nominal

al. 2012)

Water flea
Fresh
72-hour
LCso = 28.39
0, 5, 10, 20, 30, 40,
Aquatic-not reported,
Mortality
(Dobaradaran et
Medium
(Daphnia magna)


mg/L
50, 75, 100 mg/L
Nominal

al., 2012)

Water flea
Fresh
96-hour
LCso = 26.55
0, 5, 10, 20, 30, 40,
Aquatic-not reported,
Mortality
(Dobaradaran et
Medium
(Daphnia magna)


mg/L
50, 75, 100 mg/L
Nominal

al. 2012)

Water flea
Fresh
10-day
NOAEL = 0.01
0, 0, 0.0001, 0.001,
Renewal, Nominal,
Glutathione S-
(Houde et al.
High
(Daphnia magna)


mg AI/L
0.01 mg/L
Solvent: Methanol
transferase gene
expression
2015)

Water flea
Fresh
10-day
NOAEL = 0.01
0, 0, 0.0001, 0.001,
Renewal, Nominal,
Ecdysone
(Houde et al.
High
(Daphnia magna)


mg AI/L
0.01 mg/L
Solvent: Methanol
receptor A1 gene
expression
2015)

Water flea
Fresh
10-day
NOAEL = 0.01
0, 0, 0.0001, 0.001,
Renewal, Nominal,
Ecdysone
(Houde et al.
High
(Daphnia magna)


mg AI/L
0.01 mg/L
Solvent: Methanol
receptor B
isoform gene
expression
20.1.5)

Water flea
Fresh
10-day
NOAEL = 0.01
0, 0, 0.0001, 0.001,
Renewal, Nominal,
Vitellogenin 1
(Houde et al.
High
(Daphnia magna)


mg AI/L
0.01 mg/L
Solvent: Methanol
gene expression
20.1.5)

Water flea
Fresh
10-day
NOAEL = 0.01
0, 0, 0.0001, 0.001,
Renewal, Nominal,
Vitellogenin 2
(Houde et al.
High
(Daphnia magna)


mg AI/L
0.01 mg/L
Solvent: Methanol
gene expression
20.1.5)

Water flea
Fresh
10-day
NOAEL=
0, 0, 0.0001, 0.001,
Renewal, Nominal,
Chitinase activity
(Houde et al.
High
(Daphnia magna)


0.001 mg AI/L
LOAEL = 0.01
mg AI/L
0.01 mg/L
Solvent: Methanol

20.1.5)

Water flea
Fresh
10-day
NOAEL = 0.01
0, 0, 0.0001, 0.001,
Renewal, Nominal,
Vitellogenin-like
(Houde et al.
High
(Daphnia magna)


mg AI/L
0.01 mg/L
Solvent: Methanol
protein
concentration
20.1.5)

Water flea
Fresh
10-day
NOAEL = 0.01
0, 0, 0.0001, 0.001,
Renewal, Nominal,
Growth: body
(Houde et al.
High
(Daphnia magna)


mg AI/L
0.01 mg/L
Solvent: Methanol
length
20.1.5)

Water flea
Fresh
10-day
NOAEL = 0.01
0, 0, 0.0001,0.001,
Renewal, Nominal,
Reproduction:
(Houde et al.
High
(Daphnia magna)


mg AI/L
0.01 mg/L
Solvent: Methanol
Time to first
brood
20.1.5)

Water flea
Fresh
10-day
NOAEL = 0.01
0, 0, 0.0001, 0.001,
Renewal, Nominal,
Reproduction:
(Houde et al.
High
(Daphnia magna)


mg AI/L
0.01 mg/L
Solvent: Methanol
Number of
neonates
20.1.5)


-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Diirsilinn
Kiul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerences
Dsilsi Quali(>
l'\si In sit ion
Wilier Ilea
{Daphnia magna)
I'resli
l
-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qusili(>
l'\si In sit ion
1 !nno shrimp
(Artemia salina)
Sail
24-lkuir
l.( 5 ^
mg/L*
\nl ivpoi'lcd
Sialic.
Nominal, Solvent:
ethanol
Moilalilv. lor
shrimp starting at
48-hours old
(
al. 1997)
1 A»\X
Brine shrimp
(Artemia salina)
Salt
24-hour
LCso = 0.92
mg/L*
Not reported
Static,
Nominal, Solvent:
ethanol
Mortality, for
shrimp starting at
72-hours old
(Sanchez-Fortun et
al.. 1997)
Low
Asiatic clam
(Corbiculaflumine
a)
Fresh
5-day
NOAEL = 0.1 -
1.2 mg AI/L
LOAEL = <0.1
- 3.58 mg AL/L
<0.1, 1.20, 3.58,
13.99, 69.44 mg/L
(initial, day 0);
<0.1,0.10, <0.1,
0.22, 4.40 mg/L
(final, day 5)
Static, Measured
Cytochrome P-
450 level
fVidal et al. 200.1.)
High
Asiatic clam
0Corbiculaflumine
a)
Fresh
5-day
NOAEL = <0.1
-	3.58 mg AI/L
LOAEL = 0.22
-	13.99 mg AI/L
<0.1, 1.20, 3.58,
13.99, 69.44 mg/L
(initial, day 0);
<0.1, 0.10, <0.1,
0.22, 4.40 mg/L
(final, day 5)
Static, Measured
NADPH
cytochrome
reductase level
CVidal et at 2001)
High
Mysid shrimp
(Mysidopsis
bahia)
Salt
96-hour
LC50 = 27 mg
AI/L
0, 5.6, 12, 26, 69,
130 mg/L
Static, Measured
Mortality (based
on initial
measured conc.)
(Ward et al. 1986)
Medium
Mysid shrimp
{Mysidopsis
bahia)
Salt
96-hour
LC50 = 14 mg
AI/L
0, 5.6, 12, 26, 69,
130 mg/L
Static, Measured
(based on avg. of
initial/final measured
conc.)
Mortality
(Ward et al. 1986)
Medium
Mysid shrimp
{Mysidopsis
bahia)
Salt
96-hour
NOAEL = 12
mg AI/L
LOAEL = 26
mg AI/L
0, 5.6, 12, 26, 69,
130 mg/L
Static, Measured
(based on avg. of
initial/final measured
conc.)
Intoxication
(Ward et al. 1986)
Medium
Ciliate
{Tetrahymena
pyriformis)
Fresh
24-hour
EC50 = 410 mg
AI/L
Not reported
Static, Nominal
Population
growth rate
(Yoshioka et al.
1985)
Unacceptable
Flatworm
{Dugesia
japonica)
Fresh
7-day
LC50 = 1.7 mg
AI/L
Not reported
Renewal, Nominal
Mortality
(Yoshioka et al.
.1.986)
Low

-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uel'erenees
Dsilsi Qusili(>
l'\si In sit ion
I'kilvuirm
(.Dugesia
japonica)
I'resli
~-da\
IX 1 " mu
AI/L
\nl ivpoi'lcd
Renewal. Nominal
\hikirmal
regeneration
(
1986)
1 A»\X
Water flea
(.Moina
macrocopa)
Fresh
3-hour
LC50 = 2.3 mg
AI/L
Not reported
Static, Nominal
Mortality
(Yoshioka et a.L
.1.986)
Unacceptable
Algae
Green algae
{Chlamydomonas
reinhardtii)
Fresh
2-hour
ECos = 13 mg
AI/L
Not reported
Static, Nominal
Chlorophyll a
fluorescence
(Brack and Frank,
.1.998)
Unacceptable
Green algae
('Chlamydomonas
reinhardtii)
Fresh
72-hour
EC10 = 12.3 mg
AI/L
Not reported
Static, Nominal
Biomass
(Brack and Rottler.
.1.994)
High
Green algae
{Chlamydomonas
reinhardtii)
Fresh
72-hour
EC50 = 36.5 mg
AI/L
Not reported
Static, Nominal
Biomass
(Brack and Rottler,
.1.994)
High
Green algae
(Chlorella
vulgaris)
Fresh
10-day
NOAEL = 3 mg
AI/L
0, 0.003, 0.03, 0.3, 3
mg/L
Static, Nominal
Growth
(chlorophyll A
concentration)
(Ando et al, 2003)
Medium
Green algae
(Pseudokirchnerie
lla subcapitata)
Fresh
10-day
NOAEL = 3 mg
AI/L
0, 0.003, 0.03, 0.3, 3
mg/L
Static, Nominal
Growth
(chlorophyll A
concentration)
(Ando et al, 2003)
Medium
Green algae
(Volvulina steinii)
Fresh
10-day
LOAEL = 0.003
mg AI/L
0, 0.003, 0.03, 0.3, 3
mg/L
Static, Nominal
Growth
(chlorophyll A
concentration)
(Ando et al, 2003)
Medium
Green algae
(Parachlorella
kessleri)
Fresh
96-hour
EC50 = 240 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Plate method
Growth (dry
weight)
(Lukavskv et al.
:oi I)
Medium
Green algae
{Pseudokirchnerie
lla subcapitata)
Fresh
96-hour
EC50 = 450 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Plate method
Growth (dry
weight)
(Lukavskv et al..
.I'll)
Medium
Green algae
{Scenedesmus
quadicauda)
Fresh
96-hour
EC50 = 400 mg
AI/L
Not reported
Static, Nominal,
Solvent: Methanol,
Plate method
Growth (dry
weight)
(Lukavskv et al.
:oi I)
Medium

-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerences
Dsilsi Qusili(>
l'\si In sit ion
(iiven algae
(.Desmodesmus
subspicatus)
llVsh
lJ(>-lk»lll"
IX v^iimu
AI/L
11. 11- I.111111 niu 1.
Sialic. Nominal.
Solvent: Methanol,
Plate method
( ll'ow ill (dl'\
weight)
i
.I'll)
Medium
Blue-green algae
{Synechococcus
elongatus)
Fresh
96-hour
EC50 = 800 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Plate method
Growth (dry
weight)
(Lukavskv et al.„
:oi I)
Medium
Blue-green algae
{Synechococcus
leopoliensis)
Fresh
96-hour
EC50 = 300 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Plate method
Growth (dry
weight)
(Lukavskv et al.„
.I'll)
Medium
Green algae
(Parachlorella
kessleri)
Fresh
96-hour
EC50 = 430 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Growth (dry
weight)
(Lukavskv et al.„
20.1.1)
Medium
Green algae
{Parachlorella
kessleri)
Fresh
96-hour
EC50 = 700 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(O2, production)
(Lukavskv et al.
20.1.1)
Medium
Green algae
{Parachlorella
kessleri)
Fresh
96-hour
EC50 = 700 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(pH change)
(Lukavskv et al,
20.1.1)
Medium
Green algae
{Chlamydomonas
reinhardtii)
Fresh
96-hour
EC50 = 520 mg
AI/L
Not reported
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Growth (dry
weight)
(Lukavskv et al.
20.1.1)
Medium
Green algae
{Chlamydomonas
reinhardtii)
Fresh
96-hour
EC50) = 700 mg
AI/L
Not reported
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(O2, production)
(Lukavskv et al.
.I'll)
Medium
Green algae
{Chlamydomonas
reinhardtii)
Fresh
96-hour
EC50 = 700 mg
AI/L
Not reported
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(pH change)
(Lukavskv et al.
:oi I)
Medium
Green algae
{Pseudokirchnerie
lla subcapitata)
Fresh
96-hour
EC50 = 220 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Growth (dry
weight)
(Lukavskv et al.
.I'll)
Medium

-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerences
Dsilsi Qu;ili(>
l'\si In sit ion
(iiven algae
(Pseudokirchnerie
lla subcapitata)
llVsh
lJ(>-lk»lll"
IX i "		 u
AI/L
II. II- l.llllll lliu I.
Sialic. Nominal.
Solvent: Methanol,
Glass enclosure
method
Mki|os\ nihesis
(O2, production)
i
.I'll)
Medium
Green algae
{Pseudokirchnerie
lla subcapitata)
Fresh
96-hour
EC50 = 700 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(pH change)
(Lukavskv et al.
:oi I)
Medium
Green algae
(Scenedesmus
quadicauda)
Fresh
96-hour
EC50 = 500 mg
AI/L
Not reported
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Growth (dry
weight)
(Lukavskv et al.
20.1.1)
Medium
Green algae
(Scenedesmus
quadicauda)
Fresh
96-hour
EC50 = 500 mg
AI/L
Not reported
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(O2, production)
(Lukavskv et al,
20.1.1)
Medium
Green algae
(Scenedesmus
quadicauda)
Fresh
96-hour
EC50 = 600 mg
AI/L
Not reported
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(pH change)
(Lukavskv et al.
20.1.1)
Medium
Green algae
(Desmodesmus
subspicatus)
Fresh
96-hour
EC50 = 820 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Growth (dry
weight)
(Lukavskv et al,
20.1.1)
Medium
Green algae
(Desmodesmus
subspicatus)
Fresh
96-hour
EC50 = 400 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(O2, production)
(Lukavskv et al.
:oi I)
Medium
Green algae
(Desmodesmus
subspicatus)
Fresh
96-hour
EC50 = 400 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(pH change)
(Lukavskv et al.
.I'll)
Medium
Blue-green algae
(Synechococcus
elongatus)
Fresh
96-hour
EC50 = 800 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Growth (dry
weight)
(Lukavskv et al.
:oi I)
Medium

-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qu;ili(>
l'\si In sit ion
r.luc-uiv.vii algae
{Synechococcus
elongatus)
I'resli

IX <><><) inu
AI/L
I). ()- 1.000 II1U 1.
Sialic. Nominal.
Solvent: Methanol,
Glass enclosure
method
Mki|os\ iiiliesis
(O2, production)
i
.I'll)
Medium
Blue-green algae
{Synechococcus
elongatus)
Fresh
96-hour
EC50= 700 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(pH change)
(Lukavskv et al.„
:oi I)
Medium
Blue-green algae
{Synechococcus
leopoliensis)
Fresh
96-hour
EC50 = 600 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Growth (dry
weight)
(Lukavskv et al.„
20.1.1)
Medium
Blue-green algae
{Synechococcus
leopoliensis)
Fresh
96-hour
EC50 = 480 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(O2, production)
(Lukavskv et al,
20.1.1)
Medium
Blue-green algae
{Synechococcus
leopoliensis)
Fresh
96-hour
EC50 = 450 mg
AI/L
0, >0—1,000 mg/L
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(pH change)
(Lukavskv et al.
20.1.1)
Medium
Blue-green algae
{Microcystis
aeruginosa)
Fresh
96-hour
EC50 = 130 mg
AI/L
Not reported
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Growth (dry
weight)
(Lukavskv et al,
20.1.1)
Medium
Blue-green algae
{Microcystis
aeruginosa)
Fresh
96-hour
EC50 = 100 mg
AI/L
Not reported
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(O2, production)
(Lukavskv et al.
:oi I)
Medium
Blue-green algae
{Microcystis
aeruginosa)
Fresh
96-hour
EC50 = 250 mg
AI/L
Not reported
Static, Nominal,
Solvent: Methanol,
Glass enclosure
method
Photosynthesis
(pH change)
(Lukavskv et al.
.I'll)
Medium
Green algae
{Pseudokirchnerie
lla subcapitata)
Fresh
24-hour
NOAEL = 0.05
LOAEL = 0.2
0,0.01,0.02,0.05,
0.2, 0.5 mg/L
Static, Nominal
Abundance (total
algal number)
(Labra et al. 20.1.0)
Medium

-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerences
Dsilsi Qu;ili(>
l'\si In sit ion
(iiven algae
(Pseudokirchnerie
lla subcapitata)
llVsh
4X-lki|ll'
\o\i:i. (mi:
mg AI/L
LOAEL = 0.05
mg AI/L
(1. (Ml|. (1 111 (1 ()5.
0.2, 0.5 mg/L
Sialic. Nominal
\himdaiice iioial
algal number)
( 1
Medium
Green algae
{Pseudokirchnerie
lla subcapitata)
Fresh
72-hour
NOAEL = 0.02
mg AI/L
LOAEL = 0.05
mg AI/L
0,0.01,0.02,0.05,
0.2, 0.5 mg/L
Static, Nominal
Abundance (total
algal number)
(Labra et aL 2010)
Medium
Green algae
{Pseudokirchnerie
lla subcapitata)
Fresh
72-hour
NOAEL = 0.05
mg AI/L
LOAEL = 0.2
mg AI/L
0,0.01,0.02,0.05,
0.2, 0.5 mg/L
Static, Nominal
Damage/ FDA
fluorescence
(Labra et aL 2010)
Medium
Green algae
{Pseudokirchnerie
lla subcapitata)
Fresh
48-hour
NOAEL = 0.02
mg AI/L
LOAEL = 0.05
mg AI/L
0,0.01,0.02,0.05,
0.2, 0.5 mg/L
Static, Nominal
Damage/ FDA
fluorescence
(Labra et aL. 2010)
Medium
Green algae
{Pseudokirchnerie
lla subcapitata)
Fresh
24-hour
NOAEL = 0.05
mg AI/L
LOAEL = 0.2
mg AI/L
0,0.01,0.02,0.05,
0.2, 0.5 mg/L
Static, Nominal
Damage/ FDA
fluorescence
(Labra et aL, 2010)
Medium
Diatom
{Skeletonema
costatum)
Salt
96-hour
EC50 = 150 mg
AI/L (based on
initial meas.
conc.)
0, 8.5, 12, 35,64,
150 mg/L
Static, Measured
Chlorophyll a,
Cell count
(Ward et aL. 1986)
Medium
Diatom
{Skeletonema
costatum)
Salt
96-hour
EC50 = 95 mg
AI/L (based on
average
measured conc.)
0, 8.5, 12, 35, 64,
150 mg/L
Static, Measured
Chlorophyll a,
Cell count
(Ward et aL. 1986)
Medium
Blue-green algae
{Synechococcus
elongatus)
Fresh
12-hour
LOAEL = 1367
mg/L
0, 1367 mg/L
Static, Nominal
Peroxidase
activity
(Bacsi et aL. 2013)
Unacceptable
Blue-green algae
{Synechococcus
elongatus)
Fresh
24-hour
LOAEL = 1367
mg/L
0, 1367 mg/L
Static, Nominal
Thiobarbituric
acid reactive
substances,
Superoxide
dismutase
activities
(Bacsi et aL. 2013)
Unacceptable

-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qu;ili(>
l'\si In sit ion
r.luc-uiv.vii algae
(Synechococcus
elongatus)
I'resli
12-lkmi'
i.o\i:i. n<._
mg/L
<>. i i
-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qu;ili(>
l'\si In sit ion
r.luc-uiv.vii algae
(Synechococcus
elongatus)
I'resli
4-Ikmii"
i.o\i:i. n<._
mg/L
<>. i i
-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qusili(>
l'\si In sit ion
Music Mk»l
reported)
Ircsli
:-da>
i.o\i:i. i. -(.(.
mg AI/L
<>. 1.1(1(1 mg 1.
1 c 1 ilic. Nominal
1 )i\ cimi\ .
Evenness (2011
assemblages)
( 1
I nacccpiahlc
Algae (not
reported)
Fresh
3-day
LOAEL = 1,366
mg AI/L
0, 1,366 mg/L
Lentic, Nominal
Diversity,
Evenness (2011
assemblages)
(Bacsi et aL 2015)
Unacceptable
Algae (not
reported)
Fresh
1-day
LOAEL = 1,366
mg AI/L
0, 1,366 mg/L
Lentic, Nominal
Chlorophyll A
concentration
(Bacsi et aL 2015)
Unacceptable
Algae (not
reported)
Fresh
2-day
LOAEL = 1,366
mg AI/L
0, 1,366 mg/L
Lentic, Nominal
Algal abundance
(individual #;
2012
assemblages)
(Bacsi et aL 2015)
Unacceptable
Algae (not
reported)
Fresh
3-day
NOAEL =
1,366 mg AI/L
0, 1,366 mg/L
Lentic, Nominal
Algal bundance
(individual #;
2011
assemblages)
(Bacsi et aL. 20.1.5)
Unacceptable
Algae (not
reported)
Fresh
1-day
NOAEL=
1,366 mg AI/L
0, 1,366 mg/L
Lentic, Nominal
Diversity,
evenness (2012
assemblages)
(Bacsi et aL. 20.1.5)
Unacceptable
Algae (not
reported)
Fresh
2-day
NOAEL=
1,366 mg AI/L
0, 1,366 mg/L
Lentic, Nominal
Diversity,
evenness (2012
assemblages)
(Bacsi et aL. 20.1.5)
Unacceptable
Algae (not
reported)
Fresh
3-day
LOAEL = 1,366
mg AI/L
0, 1,366 mg/L
Lentic, Nominal
Diversity,
evenness (2012
assemblages)
(Bacsi et aL. 20.1.5)
Unacceptable
Green algae
(Pseudokirchnerie
lla subcapitata)
Fresh
48-hour
ECso = 26.24
mg AI/L
Not reported
Static, Nominal
Population
growth rate
(Tsai and Chen.
2007)
High
Green algae
(Pseudokirchnerie
lla subcapitata)
Fresh
NA
No data
reported; test
discontinued
NA
NA
Population
growth rate
(U inweltbiindesa in
t. .1.984)
Unacceptable
Amphibians
African clawed
frog (Xenopus
laevis)
Fresh
96-hour
LC50 = 434 mg
AI/L
Not reported
Renewal, Nominal,
Solvent: DMSO,
without activation
Mortality
(Fort et aL. .1.993)
High

-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qusili(\
l'\si In sit ion
\liicaii clawed
frog (Xenopus
laevis)
I'resli

l!( "(> niu
AI/L
\nl ivpoi'lcd
Renewal. Nominal.
Solvent: DMSO,
without activation
1 eiala
( )
1 huh
African clawed
frog (Xenopus
laevis)
Fresh
96-hour
Minimum
Concentrations
to Inhibit
Growth (MCIG)
= 29 mg AI/L
Not reported
Renewal, Nominal,
Solvent: DMSO,
without activation
Embryo growth
(Tort ef al.. 19931
High
African clawed
frog (Xenopus
laevis)
Fresh
96-hour
LC50 = 241.0
mg AI/L
Not reported
Renewal, Nominal,
Solvent: DMSO, with
activation
Mortality
(Fort et al. 1993)
High
African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 9.5 mg
AI/L
Not reported
Renewal, Nominal,
Solvent: DMSO, with
activation
Terata
(Fort et al. 1993)
High
African clawed
frog (Xenopus
laevis)
Fresh
96-hour
MCIG = 9.0 mg
AI/L
Not reported
Renewal, Nominal,
Solvent: DMSO, with
activation
Embryo growth
(Fort et al.. 1993)
High
African clawed
frog (Xenopus
laevis)
Fresh
96-hour
LC50 = 398 mg
AI/L
Not reported
Renewal, Nominal,
Solvent: DMSO, with
carbon monoxide-
inhibited activation
Mortality
(Fort et al.. 1993)
High
African clawed
frog (Xenopus
laevis)
Fresh
96-hour
EC50 = 22 mg
AI/L
Not reported
Renewal, Nominal,
Solvent: DMSO, with
carbon monoxide-
inhibited activation
Terata
(Fort et al. 1993)
High
African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 20 mg
AI/L
Not reported
Renewal, Nominal,
Solvent: DMSO, with
carbon monoxide-
inhibited activation
Embryo growth
(Fort et al. 1993)
High
African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50 = 39 mg
AI/L
Not reported
Renewal, Nominal,
Solvent: DMSO, with
cyclohexene oxide-
inhibited activation
Mortality
(Foil et al. 1993)
High
African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 0.07 mg
AI/L
Not reported
Renewal, Nominal,
Solvent: DMSO, with
cyclohexene oxide-
inhibited activation
Terata
(Fort et al, 1993)
High

-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qusili(\
l'\si In sit ion
\liicaii clawed
frog (Xenopus
laevis)
I'resli

\1( 			
mg AI/L
\nl ivpoi'lcd
Renewal. Nominal.
Solvent: DMSO, with
cyclohexene oxide-
inhibited activation
1 Jiihiwi ui'ouih
( )
1 huh

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
LC50 = 412 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
without activation
Mortality
(Fort ef aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 40.9 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
without activation
Developmental:
Deformation
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 150
mg AI/L (Test
1)
Not reported
Renewal, Nominal,
without activation
Embryo growth
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50 = 426 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
without activation
Mortality
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 36.2 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
without activation
Developmental:
Deformation
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 150
mg AI/L (Test
2)
Not reported
Renewal, Nominal,
without activation
Embryo growth
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50 = 490 mg
AI/L (Prelim.
Test)
Not reported
Renewal, Nominal,
without activation
Mortality
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 51 mg
AI/L (Prelim.
Test)
Not reported
Renewal, Nominal,
without activation
Developmental:
Deformation
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 400
mg AI/L
(Prelim. Test)
Not reported
Renewal, Nominal,
without activation
Embryo growth
(Fort et aL, 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50 = 378.1
mg AI/L (Test
1)
Not reported
Renewal, Nominal,
Activation (Aroclor
1254)
Mortality
(Fort et aL. 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 40.2 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (Aroclor
1254)
Developmental:
Deformation
(Fort et aL. 2001)
Medium


-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qiisili(>
l'\si In sit ion
\liicaii clawed
frog (Xenopus
laevis)
I'resli

\1( l(i I5U
mg AI/L (Test
1)
\nl ivpoi'lcd
Renewal. Nominal.
Activation (Aroclor
1254)
1 Jiihiwi ui'ouih
( )
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
LC50 = 412.1
mg AI/L (Test
1)
Not reported
Renewal, Nominal,
Activation
(Phenobarbital)
Mortality
(Fort ef aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 18 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation
(Phenobarbital)
Developmental:
Deformation
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 150
mg AI/L (Test
1)
Not reported
Renewal, Nominal,
Activation
(Phenobarbital)
Embryo growth
(Fort et aL. 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50 = 390.1
mg AI/L (Test
1)
Not reported
Renewal, Nominal,
Activation(P-
Naphthoflavone)
Mortality
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 39.7 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation(P-
Naphthoflavone)
Developmental:
Deformation
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 150
mg AI/L (Test
1)
Not reported
Renewal, Nominal,
Activation(P-
Naphthoflavone)
Embryo growth
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50 = 190.4
mg AI/L (Test
1)
Not reported
Renewal, Nominal,
Activation (Isoniazid)
Mortality
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 11.9 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (Isoniazid)
Developmental:
Deformation
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 50 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (Isoniazid)
Embryo growth
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50= 181.3
mg AI/L (Test
1)
Not reported
Renewal, Nominal,
Activation (mixed)
Mortality
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 7.5 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (mixed)
Developmental:
Deformation
(Fort et aL 2001)
Medium


-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qiisili(>
l'\si In sit ion
\liicaii clawed
frog (Xenopus
laevis)
I'resli

MCIG 5(i mu
AI/L (Test 1)
\nl ivpoi'lcd
Renewal. Nominal.
Activation (mixed)
1 Jiihiwi ui'ouih
( )
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
LC50 = 3 83.5
mg AI/L (Test
2)
Not reported
Renewal, Nominal,
Activation (Aroclor
1254)
Mortality
(Fort ef aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 37.3 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation (Aroclor
1254)
Developmental:
Deformation
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 150
mg AI/L (Test
2)
Not reported
Renewal, Nominal,
Activation (Aroclor
1254)
Embryo growth
(Fort et aL. 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC5o = 410.8
mg AI/L (Test
2)
Not reported
Renewal, Nominal,
Activation
(Phenobarbital)
Mortality
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 19.5 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation
(Phenobarbital)
Developmental:
Deformation
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 100
mg AI/L (Test
2)
Not reported
Renewal, Nominal,
Activation
(Phenobarbital)
Embryo growth
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50 = 388.9
mg AI/L (Test
2)
Not reported
Renewal, Nominal,
Activation ((3-
Naphthoflavone)
Mortality
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 40.2 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation ((3-
Naphthoflavone)
Developmental:
Deformation
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 150
mg AI/L (Test
2)
Not reported
Renewal, Nominal,
Activation ((3-
Naphthoflavone)
Embryo growth
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LCso = 175.3
mg AI/L (Test
2)
Not reported
Renewal, Nominal,
Activation (Isoniazid)
Mortality
(Fort et aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 11.5 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation (Isoniazid)
Developmental:
Deformation
(Fort et aL 2001)
Medium


-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qiisili(>
l'\si In sit ion
\liicaii clawed
frog (Xenopus
laevis)
I'resli

\1( l(i 5(i mu
AI/L (Test 2)
\nl ivpoi'lcd
Renewal. Nominal.
Activation (Isoniazid)
1 Jiihiwi ui'ouih
( )
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
LC50= 169.1
mg AI/L (Test
2)
Not reported
Renewal, Nominal,
Activation (mixed)
Mortality
(Fort ef aL 2001)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 7.6 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation (mixed)
Developmental:
Deformation
(Fort et aL 2001)
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
MCIG = 25 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation (mixed)
Embryo growth
(Fort et aL. 2001)
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
LC50 = 425 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
without activation
Mortality
(Fort et aL 1991)
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
EC50 = 34 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
without activation
Terata
(Fort et aL 1991)
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
LC50 = 443 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
without activation
Mortality
(Fort et aL 1991)
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
EC50 = 37 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
without activation
Terata
(Fort et aL 1991)
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
MCIG = 29 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
without activation
Embryo growth
(Fort et aL 1991)
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
LC50 = 423 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (Aroclor
1254)
Mortality
(Fort et aL 1991)
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
EC50 = 45 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (Aroclor
1254)
Terata
(Fort et aL 1991)
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
LC50 = 397 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation (Aroclor
1254)
Mortality
(Fort et aL 1991)
Medium


-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uel'erenees
Dsilsi Qusili(>
l'\si In sit ion
\liicaii clawed
frog (Xenopus
laevis)
I'resli

i:c r in-
AI/L (Test 2)
\nl ivpoi'lcd
Renewal. Nominal.
Activation (Aroclor
1254)
1 erala
( 1
Medium

African clawed
frog (Xenopus
laevis)
Fresh
96-hour
MCIG = 29 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (Aroclor
1254)
Embryo growth
(Fort ef aL 1991)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG= 37 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation (Aroclor
1254)
Embryo growth
(Fort et aL 1991)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50 = 261 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (Isoniazid)
Mortality
(Fort et aL. 1991)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 9 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (Isoniazid)
Terata
(Fort et aL 1991)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50 = 228 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation (Isoniazid)
Mortality
(Fort et aL, 1991)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 10 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation (Isoniazid)
Terata
(Fort et aL. 1991)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 13 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (Isoniazid)
Embryo growth
(Fort et aL. 1991)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 9 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation (Isoniazid)
Embryo growth
(Fort et aL. 1991)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50 = 249 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (mixed)
Mortality
(Fort et aL. 1991)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
EC50 = 13 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (mixed)
Terata
(Fort et aL. 1991)
Medium

African clawed
frog {Xenopus
laevis)
Fresh
96-hour
LC50 = 204 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation (mixed)
Mortality
(Fort et aL. 1991)
Medium


-------
PEER REVIEW DRAFT - DO NOT CITE OR QUOTE
1 esl Species
Ircsli/
Siili
\\ silcr
Dursilion
l-liul-pninl
(oneenlrsilionis)
Tesl Aii;il> sis
r.lTecl(s)
Uelerenees
Dsilsi Qiisili(>
l'\si In sit ion
\liicaii clawed
frog (Xenopus
laevis)
I'resli

i:r ') niu
AI/L (Test 2)
\nl ivpoi'lcd
Renewal. Nominal.
Activation (mixed)
1 erala
( )
Medium
African clawed
frog (Xenopus
laevis)
Fresh
96-hour
MCIG = 11 mg
AI/L (Test 1)
Not reported
Renewal, Nominal,
Activation (mixed)
Embryo growth
(Fort ef aL 1991)
Medium
African clawed
frog {Xenopus
laevis)
Fresh
96-hour
MCIG = 7 mg
AI/L (Test 2)
Not reported
Renewal, Nominal,
Activation (mixed)
Embryo growth
(Fort et aL 1991)
Medium
Clawed frog
(Xenopus sp.)
Fresh
96-hour
LC50 = 350 mg
AI/L (Test 1)
Not reported
Static, Nominal
Mortality
(Ravburn et aL
1991)
Unacceptable
Clawed frog
(Xenopus sp.)
Fresh
96-hour
EC50 = 70.1 mg
AI/L (Test 1)
Not reported
Static, Nominal
Malformation
(Ravburn et aL
1991)
Unacceptable
Clawed frog
(Xenopus sp.)
Fresh
96-hour
LC50 = 423 mg
AI/L (Test 2)
Not reported
Static, Nominal
Mortality
(Ravburn et aL
1991)
Unacceptable
Clawed frog
(Xenopus sp.)
Fresh
96-hour
EC50 = 33.6 mg
AI/L (Test 2)
Not reported
Static, Nominal
Malformation
(Ravburn et aL
1991)
Unacceptable
Clawed frog
(Xenopus sp.)
Fresh
96-hour
MCIG = 292
mg AI/L
Not reported
Static, Nominal
Embryo growth
(Ravburn et aL
1991)
Unacceptable
Clawed frog
(Xenopus sp.)
Fresh
96-hour
LC25 = 511 mg
AI/L
Not reported
Static, Nominal
Mortality
(Ravburn et aL
1991)
Unacceptable
Clawed frog
(Xenopus sp.)
Fresh
96-hour
EC25 = 29 mg
AI/L
Not reported
Static, Nominal
Abnormal
(Ravburn et aL
1991)
Unacceptable
Wood frog
(Lithobates
sylvaticus)
Fresh
96-hour
EC50 = 32.2 mg
AI/L
0, 12.5, 20, 40, 60
mg/L
Renewal, Measured
Developmental:
Deformation
(McDaniel et aL,
2004)
High
Green frog (Rana
clamitans)
Fresh
96-hour
EC50 = 22 mg
AI/L
0, 12.5, 20, 40, 60
mg/L
Renewal, Measured
Developmental:
Deformation
(McDaniel et aL
2004)
High
American toad
(Bufo americanus)
Fresh
96-hour
EC50 >85 mg
AI/L
0, 12.5, 20, 35, 40,
55, 60, 85 mg/L
Renewal, Nominal
Developmental:
Deformation
(McDaniel et aL
2004)
High
Spotted
salamander
(Ambystoma
maculatum)
Fresh
96-hour
EC50 = 40 mg
AI/L
0, 12.5, 20, 40, 60
mg/L
Renewal, Measured
Developmental:
Deformation
(McDaniel et aL
2004)
Medium

-------