beta- Propiolactone
57-57-8
Hazard Summary
beta-Propiolactone is used for vaccines, tissue grafts, surgical instruments, and enzymes, as a sterilant of
blood plasma, water, milk, and nutrient broth, and as a vapor-phase disinfectant in enclosed spaces. Acute
(short-term) inhalation exposure to beta-propiolactone causes severe irritation of the eyes, nose, throat,
and respiratory tract in humans. Acute dermal exposure may cause irritation of the skin, blistering, or
burns in humans. Contact with the eyes may cause permanent corneal opacification. Burns of the mouth
and stomach may occur in humans following acute exposure via ingestion. No information is available on
the chronic (long-term), reproductive, developmental, or carcinogenic effects of beta-propiolactone in
humans. Squamous cell carcinomas of the forestomach have been reported in orally exposed rats. In
dermally exposed rodents, skin tumors have been observed. The International Agency for Research on
Cancer (IARC) has classified beta-propiolactone as a Croup 2B, possible human carcinogen.
Please Note: The main sources of information for this fact sheet are the Hazardous Substances Data Bank (HSDB)
(1), a database of summaries of peer-reviewed literature, and the IARC monographs on chemicals carcinogenic to
humans. (6)
Uses
s beta-Propiolactone is used for vaccines, tissue grafts, surgical instruments, and enzymes, as a sterilant of
blood plasma, water, milk, and nutrient broth, and as a vapor-phase disinfectant in enclosed spaces. Its
sporicidal action is used against vegetative bacteria, pathologic fungi, and viruses. (1,2,8)
¦ beta-Propiolactone is also used as a chemical intermediate. (1,2,7)
Sources and Potential Exposure
s Occupational exposure to beta-propiolactone may occur through inhalation or dermal contact during its
manufacture and use. (1)
Assessing Personal Exposure
• No information was located regarding the measurement of personal exposure to beta-propiolactone.
Health Hazard Information
Acute Effects:
a Acute inhalation exposure beta-propiolactone causes severe irritation of the eyes, nose, throat, and
respiratory tract in humans. Acute dermal exposure may cause irritation of the skin, blistering, or burns in
humans. Contact with the eyes may cause permanent corneal opacification. Burns of the mouth and
stomach may occur in humans following acute exposure via ingestion. (1,2)
a Acute oral exposure has been observed to result in muscular spasms, respiratory difficulty, and convulsions
at high levels in rats. In rats acutely exposed intravenously, liver and kidney tubular damage has been
reported. (1,3)
a Acute animal exposure tests in rats have demonstrated beta-propiolactone to have extreme acute toxicity
by inhalation. (4)

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Chronic Effects (Noncancer):
s No information is available on the chronic health effects of beta-propiolactone in humans.
s Chronic dermal exposure has resulted in skin irritation, scarring, and hair loss in mice. (1,3)
a EPA has not established a Reference Concentration (RfC) or a Reference Dose (RfD) for beta-
propiolactone.(2)
Reproductive/Develop mental Effects:
•	No information is available on the reproductive or developmental effects of beta-propiolactone in humans
or animals.
Cancer Risk:
a No information is available on the carcinogenic effects of beta-propiolactone in humans,
a Squamous cell carcinomas of the forestomach have been reported in orally exposed rats. (1,6,8)
a In several studies of rats and mice exposed to beta-propiolactone via subcutaneous injection, local tumors
have been observed at the site of injection. Lymphomas and hepatomas have been reported in mice
following intraperitoneal injection. (1,6,8)
a In mice, hamsters, and guinea pigs dermally exposed, skin tumors have been observed. (1,6,8)
a EPA has not classified beta-propiolactone for carcinogenicity. (2)
a IARC has classified beta-propiolactone as a Group 2B, possible human carcinogen. (6)
Physical Properties
a The chemical formula for beta-propiolactone is C H O , and its molecular weight is 72.06 g/mol. (7)
3 4 2
•	beta-Propiolactone occurs as a colorless liquid th at i s v ery soluble in water. (1,6)
a beta-Propiolactone has a pungent, slightly sweetish smell; the odor threshold has not been
established.(1,2,6)
a The vapor pressure for beta-propiolactone is 3.4 mm Hg at 2 5 °C, and its log octanol/water partition
coefficient (log K ) is 0.462. (1,6)
ow
Conversion Factors:
3	3
To convert concentrations in air (at 2 5 °C) from ppm to mg/m : m^/m = (ppm) x (molecular weight of the
compound)/(24.45). For beta-propiolactone: 1 ppm = 2.95 mg/m .
Health Data from Inhalation Exposure

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I
g
'B
¦S
fj
5
Propiolactone
1000
100
10
0.1
OjOI
Health
numbers"
LCb fats) (74 nrg/m3)
Regulatory, advisory
numbers'*
ACGIH TLVfl .5) rng/rn3)
ACGIH TLV—American Conference of Governmental and Industrial Hygienists' threshold limit value expressed as a
time-weighted average; the concentration of a substance to which most workers can be exposed without adverse
effects.
LC (Lethal Concentration )—A calculated concentration of a chemical in air to which exposure for a specific
50	50
length of time is expected to cause death in 50% of a defined experimental animal population.
The health and regulatory values cited in this factsheet were obtained in December 1999.
a
Health numbers are toxicological numbers from animal testing or risk assessment values developed by EPA.
b
Regulatory numbers are values that have been incorporated in Government regulations, while advisory numbers
are nonregulatory values provided by the Government or other groups as advice. ACGIH numbers are advisory.
Summary created in April 1 992, updated in January 2000
References
1.	U.S. Department of Health and Human Services. Hazardous Substances Databank (HSDB, online database).
National Toxicology Information Program, National Library of Medicine, Bethesda, MD. 1 993.
2.	U.S. Environmental Protection Agency. Integrated Risk Information System (IRIS) on beta-Propiolactone.
National Center for Environmental Assessment, Office of Research and Development, Washington, DC.
1999.
3.	M. Sittig. Handbook of Toxic and Hazardous Chemicals and Carcinogens. 2nd ed. Noyes Publications, Park
Ridge, NJ. 1985.
4.	U.S. Department of Health and Human Services. Registry of Toxic Effects of Chemical Substances (RTECS,
online database). National Toxicology Information Program, National Library of Medicine, Bethesda, MD.

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1993.
5.	American Conference of Governmental Industrial Hygienists (ACCIH). 1 999 TLVs and BEIs. Threshold Limit
Values for Chemical Substances and Physical Agents. Biological Exposure Indices. Cincinnati, OH. 1 999.
6.	International Agency for Research on Cancer (IARC). IARC Monographs on the Evaluation of the
Carcinogenic Risk of Chemicals to Man: Some Aromatic Amines, Hydrazine and Related Substances, N-
Nitroso Compounds and Miscellaneous Alkylating Agents. Volume 4. World Health Organization, Lyon.
1974.
7.	The Merck Index. An Encyclopedia of Chemicals, Drugs, and Biologicals. 1 1 th ed. Ed. S. Budavari. Merck
and Co. Inc., Rahway, NJ. 1 989.
8.	U.S. Department of Health and Human Services (DHHS). The 8th Report ors Carcinogens, 1 998
Summary. Public Health Service, National Toxicology Program. Research Triangle Park, NC. 1 998.

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