Screening-Level Hazard Characterization SPONSORED CHEMICAL Oxirane, Reaction Products with Ammonia, Distillation Residues (CASRN 68953-70-8) SUPPORTING CHEMICAL Triethanolamine (CASRN 102-71-6)

U.S. Environmental Protection Agency September, 2014
Hazard Characterization Document
SCREENING-LEVEL HAZARD CHARACTERIZATION
SPONSORED CHEMICAL
Oxirane, Reaction Products with Ammonia, Distillation Residues
(CASRN 68953-70-8)
SUPPORTING CHEMICAL
T riethanolamine
(CASRN 102-71-6)
The High Production Volume (HPV) Challenge Program1 was conceived as a voluntary initiative
aimed at developing and making publicly available screening-level health and environmental
effects information on chemicals manufactured in or imported into the United States in quantities
greater than one million pounds per year. In the Challenge Program, producers and importers of
HPV chemicals voluntarily sponsored chemicals; sponsorship entailed the identification and
initial assessment of the adequacy of existing toxicity data/information, conducting new testing if
adequate data did not exist, and making both new and existing data and information available to
the public. Each complete data submission contains data on 18 internationally agreed to "SIDS"
(Screening Information Data Set l 2) endpoints that are screening-level indicators of potential
hazards (toxicity) for humans or the environment.
The Environmental Protection Agency's Office of Pollution Prevention and Toxics (OPPT) is
evaluating the data submitted in the HPV Challenge Program on approximately 1400 sponsored
chemicals by developing hazard characterizations (HCs). These HCs consist of an evaluation of
the quality and completeness of the data set provided in the Challenge Program submissions.
They are not intended to be definitive statements regarding the possibility of unreasonable risk of
injury to health or the environment.
The evaluation is performed according to established EPA guidance2 3 and is based primarily on
hazard data provided by sponsors; however, in preparing the hazard characterization, EPA
considered its own comments and public comments on the original submission as well as the
sponsor's responses to comments and revisions made to the submission. In order to determine
whether any new hazard information was developed since the time of the HPV submission, a
search of the following databases was made from one year prior to the date of the HPV
Challenge submission to the present: (ChemID to locate available data sources including
Medline/PubMed, Toxline, HSDB, IRIS, NTP, AT SDR, IARC, EXTOXNET, EPA SRS, etc.),
STN/CAS online databases (Registry file for locators, ChemAbs for toxicology data, RTECS,
Merck, etc.) Science Direct and ECHA4. OPPT's focus on these specific sources is based on their
being of high quality, highly relevant to hazard characterization, and publicly available.
OPPT does not develop HCs for those HPV chemicals which have already been assessed
internationally through the HPV program of the Organization for Economic Cooperation and
1 U.S. EPA. High Production Volume (HPV) Challenge Program; http://www.epa.gov/chemrtk/index.htm.
2 U.S. EPA. HPV Challenge Program - Information Sources; http://www.epa.gov/chemrtk/pubs/general/guidocs.htm.
3 U.S. EPA. Risk Assessment Guidelines; http://cfpub.epa.gov/ncea/raf/rafguid.cfm.
4 European Chemicals Agency, http://echa.europa.eu.

-------
U.S. Environmental Protection Agency	September, 2014
Hazard Characterization Document
Development (OECD) and for which Screening Initial Data Set (SIDS) Initial Assessment
Reports (SIAR) and SIDS Initial Assessment Profiles (SIAP) are available. These documents are
presented in an international forum that involves review and endorsement by governmental
authorities around the world. OPPT is an active participant in these meetings and accepts these
documents as reliable screening-level hazard assessments.
These hazard characterizations are technical documents intended to inform subsequent decisions
and actions by OPPT. Accordingly, the documents are not written with the goal of informing the
general public. However, they do provide a vehicle for public access to a concise assessment of
the raw technical data on HPV chemicals and provide information previously not readily
available to the public.
2

-------
U.S. Environmental Protection Agency	September, 2014
Hazard Characterization Document
Chemical Abstract Service Registry Number
(CASRN)
Sponsored Chemical
68953-70-8
Supporting Chemical
102-71-6
Chemical Abstract Index Name
Sponsored Chemical
Oxirane, reaction products with ammonia,
distillation residues
Supporting Chemical
Ethanol, 2,2'2"-nitrolotris-

Sponsored Chemical
OH
Structural Formula
/—N
HO—' N	\
OH Mixture
SMILES: OCCN(CCO)CCO
Supporting Chemical
r
SMILES: CCN(CC)CC
Summary
Oxirane, reaction products with ammonia, distillation residues is the resultant distillation residue
from the production of alkanolamines. It is a mixture that contains at least 80% ethanol, 2,2',2"-
nitrilotris- (triethanolamine; TEA; CASRN 102-71-6) and less than 1% ethanol, 2,2'-iminobis-
(diethanolamine; CASRN 111-42-2), with the remainder described as higher boiling amine
reaction products. This mixture is an amber to dark brown liquid whose primary components
possess low vapor pressure and high water solubility. The components of oxirane, reaction
products with ammonia, distillation residues are expected to possess high mobility in soil. No
biodegradation studies were available for this mixture; however, the primary component of this
mixture, ethanol, 2,2',2"-nitrilotris-, was rapidly degraded under environmental conditions using
river water and soil samples. Volatilization is expected to be low since the substances in this
mixture will primarily exist as cations under environmental conditions and cations do not
volatilize. The rate of hydrolysis is negligible since the components of this mixture do not
contain functional groups that are expected to hydrolyze under environmental conditions.
Oxirane, reaction products with ammonia, distillation residues is not readily biodegradable. The
3

-------
U.S. Environmental Protection Agency
Hazard Characterization Document
September, 2014
overall weight of evidence suggests that the components of oxirane, reaction products with
ammonia, distillation residues are expected to have low persistence (PI) and low
bioaccumulation potential (Bl).
The acute toxicity of oxirane, reaction products with ammonia, distillation residues is low in rats
via the oral and dermal routes. Oxirane, reaction products with ammonia, distillation residues
was not mutagenic in bacteria in vitro, did not induce micronuclei in mice when administered
via intraperitoneal injection in vivo and did not induce unscheduled DNA synthesis in rat
hepatocytes in vitro. Oxirane, reaction products with ammonia, distillation residues is irritating
to rabbit skin and eyes, but is not sensitizing to guinea pig skin.
The 96-h LC50 value for fish exposed to Oxirane, reaction products with ammonia, distillation
residues is 1,180 mg/L based on the supporting chemical, TEA. The 48-h EC50 value for aquatic
invertebrates exposed to oxirane, reaction products with ammonia, distillation residues is 610
mg/L based on the supporting chemical TEA. There are no adequate data to assess the toxicity
to aquatic plants.
The repeated-dose, reproductive and developmental toxicity endpoints, and toxicity to aquatic
plants were identified as data gaps under the HPV Challenge Program.	
4

-------
U.S. Environmental Protection Agency September, 2014
Hazard Characterization Document
The sponsor, Huntsman Petrochemical Corporation, submitted a Test Plan and Robust
Summaries to EPA for oxirane, reaction products with ammonia, distillation residues (CASRN
68953-70-8; CA Index name: oxirane, reaction products with ammonia, distillation residues) on
April 28, 2006. EPA posted the submission on the ChemRTK HPV Challenge website on
September 3, 2009 (http://www.epa.gov/hpv/pubs/summaries/oxireaprd/cl6248tc.htm). EPA
comments on the original submission were posted to the website on June 1, 2010. Public
comments were also received and posted to the website.
Justification for Supporting Chemical
In the test plan, the sponsor describes the typical composition of the sponsored substance as at
least 80% triethanolamine (TEA; CASRN 102-71-6), with less than 1% diethanolamine (CASRN
111-42-2); the remainder consists of higher boiling amine reaction products. The sponsor has
proposed the use of data for TEA to characterize the toxicity of the sponsored substance on the
basis that the sponsored substance typically contains at least 80% TEA.
EPA reserves judgment on the use of TEA as a supporting chemical for aquatic and most human
health effects endpoints, pending identification of the remaining -20% of the test substance
characterized as "higher boiling amine reaction products". Without more detailed information
on substance identity, EPA believes that the toxicity of TEA may not adequately represent that of
the sponsored substance, and EPA reserves judgment on the use of TEA data to fully
characterize toxicity endpoints for the sponsored substance. Therefore for the purposes of this
hazard characterization, the supporting chemical is not used to fulfill the requirements for human
health toxicity, but it is used to fulfill the requirements for aquatic toxicity.
1. Chemical Identity
1.1 Identification and Purity
According to the Robust Summaries, the purity of the tested oxirane, reaction products with
ammonia, distillation residues solutions is typical for marketed substances. No further
information on the solutions' purity was provided.
1.2 Physical-Chemical Properties
The physical-chemical properties of oxirane, reaction products with ammonia, distillation
residues are summarized in Table 1.
Oxirane, reaction products with ammonia, distillation, residues is an amber to dark brown liquid
whose components possess moderate vapor pressure and high water solubility. It is a mixture
that contains at least 80% ethanol, 2,2',2"-nitrilotris- (triethanolamine; TEA; CASRN 102-71-6)
and less than 1% ethanol, 2,2'-iminobis- (diethanolamine; CASRN 111-42-2), with the remainder
as higher boiling amine reaction products. It is used mainly as a concrete additive as a corrosion
inhibitor for steel reinforcements.
5

-------
U.S. Environmental Protection Agency September, 2014
Hazard Characterization Document
Table 1. Physical-Chemical Properties of Oxirane, Reaction Products with Ammonia,
Distillation Residues1
Property
Oxirane, reaction products with ammonia, distillation residues
CASRN
68953-70-8
Molecular Weight
149.2 (typical)
Physical State
Amber to dark brown liquid
Melting Point
20.5 (measured)2 3
Boiling Point
~372°C (measured)
Vapor Pressure
<0.75 mm Hg at 20°C (measured);
Dissociation Constant
pKb = 6.24 (measured)2 3
Henry's Law Constant
<1.0><10"10 atm-m3/mol (estimated)2-4
Water Solubility
1 x 106 mg/L at 25°C (measured)2-3
Log Kow
-1.0 (measured)2-3
huntsman Petrochemical Corporation. 2006. Test Plan and Robust Summary for oxirane, reaction products with
ammonia, distillation residues. Available online at
http://www.epa.gov/hpv/pubs/summaries/oxireaprd/cl6248tc.htm as of August 31, 2012.
2Data presented for ethanol, 2,2',2"-nitrilotris- (CASRN 102-71-6) which composes at least 80% of oxirane,
reaction products, with ammonia, distillation residues.
3SRC. 2012. The Physical Properties Database (PHYSPROP). SRC: Syracuse, NY. Available online at
http://www.srcinc.com/what-we-do/free-demos.aspx as of August 31, 2012.
4U.S. EPA. 2012. Estimation Programs Interface Suite™ for Microsoft® Windows, v4.10. U.S. Enviromnental
Protection Agency, Washington DC, USA. Available online at
http://www.epa.gov/opptintr/exposure/pubs/episuitedl.htm as of August 31, 2012.
2. General Information on Exposure
2.1 Production Volume and Use
Oxirane, reaction products with ammonia, distillation residues was not reported in the 2006 IUR.
2.2 Environmental Exposure and Fate
The environmental fate characteristics of oxirane, reaction products with ammonia, distillation
residues are summarized in Table 2.
The components of oxirane, reaction products with ammonia, distillation residues are expected to
possess high mobility in soil. No biodegradation studies were available for this mixture;
however, the primary component of this mixture, ethanol, 2,2',2"-nitrilotris- (triethanolamine;
TEA; CASRN 102-71-6), was rapidly degraded under environmental conditions, although it was
not readily biodegradable using a standard OECD screening test. The average half-life of
ethanol, 2,2',2"-nitrilotris- was 1.2 days using two river water samples in a river die-away test.
In sterilized control samples containing formaldehyde, <5% loss was noted in one river water
sample over 10 days, and <10% loss was observed in the other sterilized river water sample over
7 days. The half-life of ethanol, 2,2',2"-nitrilotris- was reported to range from 0.5 to 1.8 days in a
sandy loam surface soil at concentrations of 1.4-2,000 mg/kg. Ethanol, 2,2',2"-nitrilotris-
(CASRN 102-71-6) achieved 0% of its theoretical biochemical oxygen demand (BOD) after 2
weeks using an activated sludge inoculum and the modified MITI (OECD 301C) test. It was
6

-------
U.S. Environmental Protection Agency	September, 2014
Hazard Characterization Document
degraded only 4.2% over this time frame as measured by GC analysis. Volatilization is expected
to be low since the substances in this mixture will primarily exist as cations under environmental
conditions and cations do not volatilize. The rate of hydrolysis is negligible since the
components of this mixture do not contain functional groups that are expected to hydrolyze
under environmental conditions. Bioconcentration factors (BCF) of <4.9 measured in carp for
ethanol, 2,2',2"-nitrilotris- suggest that bioconcentration is low. The rate of atmospheric
photooxidation is rapid. The overall weight of evidence suggests that the components of oxirane,
reaction products with ammonia, distillation residues are expected to have low persistence (PI)
and low bioaccumulation potential (Bl).
Table 2. Environmental Fate Properties of Oxirane, Reaction Products with Ammonia,
Distillation, Residues1
Property
Oxirane, reaction products with ammonia, distillation, residues
CASRN
68953-70-8
Photodegradation Half-
life
1.2 hours (estimated)2 3
Hydrolysis Half-life
Stable
Biodegradation
Average half-life of 1.2 days in two river water samples2;
Half-life of 0.5-1.8 days in a sandy loam2;
0-4.2% after 2 weeks (not readily biodegradable, OECD 301C)2-4
Bioaccumulation
Factor
BCF = <0.4 (measured in carp at 0.25 mg/L)2-4;
BCF = <3.9 (measured in carp at 0.25 mg/L)2-4;
BAF = 0.89 (estimated)2-3
Log Koc
1.0 (estimated)2-3
Fugacity
(Level III Model)2 3
Air (%)
Water (%)
Soil (%)
Sediment (%)
<0.1
30.6
69.4
<0.1
Persistence5
PI (low)
Bi oaccumul ati on5
Bl (low)
huntsman Petrochemical Corporation. 2006. Test Plan and Robust Summary for oxirane, reaction products with
ammonia, distillation residues. Available online at
http://www.epa.gov/hpv/pubs/summaries/oxireaprd/cl6248tc.htm as of August 31, 2012.
2Data presented for ethanol, 2,2',2"-nitrilotris- (CASRN 102-71-6) which comprises at least 80% of oxirane,
reaction products with ammonia, distillation residues.
3U.S. EPA. 2012. Estimation Programs Interface Suite™ for Microsoft® Windows, v4.10. U.S. Enviromnental
Protection Agency, Washington, DC, USA. Available online at
http://www.epa.gov/opptintr/exposure/pubs/episuitedl.htm as of August 31, 2012.
4Chemical Risk Information Platform (CHRIP) database. 2012. Searchable by CASRN online at
http://www.safe.nite.go.ip/englisli/db.html as of August 22, 2012.
^Federal Register. 1999. Category for Persistent, Bioaccumulative, and Toxic New Chemical Substances. Federal
Register 64, Number 213 (November 4, 1999) pp. 60194-60204.
7

-------
U.S. Environmental Protection Agency September, 2014
Hazard Characterization Document
Conclusion: Oxirane, reaction products with ammonia, distillation residues is the resultant
distillation residue from the production of alkanolamines. It is a mixture that contains at least
80% ethanol, 2,2',2"-nitrilotris- (triethanolamine; TEA; CASRN 102-71-6) and less than 1%
ethanol, 2,2'-iminobis- (diethanolamine; CASRN 111-42-2), with the remainder described as
higher boiling amine reaction products. This mixture is an amber to dark brown liquid whose
primary components possess low vapor pressure and high water solubility. The components of
oxirane, reaction products with ammonia, distillation residues are expected to possess high
mobility in soil. No biodegradation studies were available for this mixture; however, the primary
component of this mixture, ethanol, 2,2',2"-nitrilotris-, was rapidly degraded under
environmental conditions using river water and soil samples. Volatilization is expected to be
low since the substances in this mixture will primarily exist as cations under environmental
conditions and cations do not volatilize. The rate of hydrolysis is negligible since the
components of this mixture do not contain functional groups that are expected to hydrolyze
under environmental conditions. The overall weight of evidence suggests that the components of
oxirane, reaction products with ammonia, distillation residues are expected to have low
persistence (PI) and low bioaccumulation potential (Bl).
3. Human Health Hazard
A summary of health effects data submitted for SIDS endpoints is provided in Table 3.
Acute Oral Toxicity
Oxirane, reaction products with ammonia, distillation, residues (CASRN 68953-70-8)
(1) Sprague-Dawley rats (5/sex) were administered 1000 mg/kg of undiluted CASRN 68953-70-
8 via gavage and observed at 1 and 4 hours after dosing, then once daily for 14 days. No
mortalities were observed. The Robust Summary states that the LD50 > 5000 mg/kg, but the
reported dosing does not support such finding.
LD50 > 1000 mg/kg
(2) Fischer 344 rats (3 females) were administered CASRN 68953-70-8 via gavage at 2000
mg/kg and observed for 14 days. No mortalities were observed.
LD50 > 2000 mg/kg
Acute Dermal Toxicity
Oxirane, reaction products with ammonia, distillation, residues (CASRN 68953-70-8)
New Zealand White rabbits (5/sex) were administered CASRN 68953-70-8 via the dermal route
at 3000 mg/kg under occluded conditions and observed for 14 days. No mortalities were
observed.
LD50 > 3000 mg/kg
8

-------
U.S. Environmental Protection Agency
Hazard Characterization Document
Repeated-Dose Toxicity
September, 2014
No adequate data
Reproductive Toxicity
No adequate data
Developmental Toxicity
No adequate data
Genetic Toxicity — Gene Mutation
In vitro
Oxirane, reaction products with ammonia, distillation residues (CASRN 68953-70-8)
In a reverse mutation assay, Salmonella typhimurium strains TA98, TA100, TA1535, TA1537
and TA1538 were exposed to CASRN 68953-70-8 at 0, 167, 500, 1670, 5000, 7500 or 10,000
|ig/plate with and without metabolic activation. Positive and negative controls were included
and responded appropriately. Cytotoxicity was not observed. Mutagenic responses were not
observed in any of the strains.
CASRN 68953-70-8 was not mutagenic in this assay.
Genetic Toxicity — Chromosomal Aberrations
In vivo
Oxirane, reaction products with ammonia, distillation, residues (CASRN 68953-70-8)
In a micronucleus assay, CD-I mice (15/sex/dose) were administered CASRN 68953-70-8 in
distilled water at 1000 mg/kg via intraperitoneal injection and 5/sex were sacrificed 24, 48 or 72
hours after dosing. Positive and negative controls were used and responded appropriately.
Treatment with the test substance did not increase the frequency of micronuclei compared to the
negative control.
CASRN 68953-70-8 did not induce micronuclei in this study.
Genetic Toxicity — Other
In vitro
Oxirane, reaction products with ammonia, distillation, residues (CASRN 68953-70-8)
In an unscheduled DNA synthesis (UDS) assay, rat hepatocytes were exposed to CASRN 68953-
70-8 at 0, 0.5, 5, 25, 50, 100, 500, 600, 750 or 5000 |ig/mL without metabolic activation.
9

-------
U.S. Environmental Protection Agency September, 2014
Hazard Characterization Document
Cytotoxicity was observed at 750 |ig/mL. A marginal increase in UDS was observed at 100 and
500 |ig/mL compared to the control, but no increase was observed when the test was repeated at
concentrations between 50 and 600 |ig/mL.
CASRN 68953-70-8 did not induce unscheduled DNA synthesis in this assay.
Additional Information
Skin Irritation
Oxirane, reaction products with ammonia, distillation, residues (CASRN 68953-70-8)
(1) In the acute dermal toxicity test in rabbits described previously, all animals administered
CASRN 68953-70-8 at 3000 mg/kg displayed moderate to severe erythema. Necrosis was
observed in one female.
CASRN 68953-70-8 was severely irritating to rabbit skin in this study.
(2) Rabbits (3/sex; strain not specified) were administered undiluted CASRN 68953-70-8 (dose
level not indicated) on one abraded and two intact skin sites per rabbit under occluded
conditions. Exposure at one intact site occurred for 4 hours; exposure at the other intact site and
the abraded site occurred for 24 hours. Animals were observed at 24, 48 and 72 hours after
treatment. Following 4 hours of exposure, there was very slight erythema on 2 of 6 rabbits,
which resolved after 72 hours. Following 24 hours of exposure, slight to severe erythema was
observed in all rabbits and slight edema was observed in 5 of 6 rabbits; erythema and edema
were no longer present at intact sites after 48 hours, but were observed at abraded sites in all
rabbits until day 9. The primary irritation indices after 4 and 24 (intact and abraded combined)
hours of exposure were 0.25 and 1.62, respectively.
CASRN 68953-70-8 was severely irritating to rabbit skin in this study.
(3) One rabbit (sex and strain not specified) was administered undiluted CASRN 68953-70-8
(dose level not indicated) to intact skin for 5 consecutive days and to abraded skin 3 times.
Slight erythema was observed at the abraded site, while no erythema or edema was observed at
the intact site.
CASRN 68953-70-8 was slightly irritating to rabbit skin in this study.
Eye Irritation
Oxirane, reaction products with ammonia, distillation, residues (CASRN 68953-70-8)
Six rabbits (sex and strain not specified) were administered 0.1 mL of undiluted CASRN 68953-
70-8 in the eye and observed for 24, 48 and 72 hours following treatment. Eyes were not rinsed.
Iris irritation was observed in 4 or 6 rabbits at 1 hour after instillation, but was not present after
24 hours. Conjunctival redness (grade 2 or 3) was observed in all rabbits after 1 hour, in 5
rabbits after 24 hours, and in 1 rabbit after 48 hours; redness was not observed after 72 hours.
Conjunctival swelling (grade 2 or 3) was observed in all rabbits after 1 hour, but was not present
after 24 hours. The mean Draize score was 18.2 out of 110.
CASRN 68953-70-8 was moderately irritating to rabbit eyes in this study.
10

-------
U.S. Environmental Protection Agency
Hazard Characterization Document
September, 2014
Skin Sensitization
Oxirane, reaction products with ammonia, distillation, residues (CASRN 68953-70-8)
In a Buehler test, Hartley guinea pigs (10/sex) received 3 applications (1 per week) of 0.3 mL of
undiluted CASRN 68953-70-8 on shorn shoulder skin under occluded conditions. The challenge
exposure consisted of 0.3 mL of the undiluted test substance on a naive skin site, applied 13 days
after the last induction exposure. Skin sites were graded at 2 and 24 hours after the challenge
exposure. No erythema was observed. Positive controls responded appropriately.
CASRN 68953-70-8 was not sensitizing to guinea pigs in this study.
Conclusion: The acute toxicity of CASRN 68953-70-8 is low in rats via the oral and dermal
routes. CASRN 68953-70-8 was not mutagenic in bacteria in vitro, did not induce micronuclei
in mice when administered via intraperitoneal injection in vivo and did not induce unscheduled
DNA synthesis in rat hepatocytes in vitro. CASRN 68953-70-8 is irritating to rabbit skin and
eyes, but is not sensitizing to guinea pig skin.
Table 3. Summary of the Screening Information Data Set as
Submitted under the U.S. HPV Challenge Program - Human Health Data
Endpoint
Oxirane, reaction products with ammonia,
distillation residues
(68953-70-8)
Acute Toxicity
Oral LDso (mg/kg)
>2000
Acute Toxicity
Dermal LCso (mg/kg)
>3000
Repeated-Dose Toxicity
NOAEL/LOAEL (mg/kg-day)
No Adequate Data
Reproductive Toxicity
NOAEL/LOAEL (mg/kg-day)
No Adequate Data
Developmental Toxicity
NOAEL/LOAEL (mg/kg-day)
No Adequate Data
Genetic Toxicity - Gene Mutation
In vitro
Negative
Genetic Toxicity -
Chromosomal Aberrations
In vivo
Negative
Genetic Toxicity - Other
Unscheduled DNA synthesis
In vitro
Negative
Additional Information
Skin irritation
Eye irritation
Skin sensitization
Irritating
Irritating
Negative
Measured data in bold
11

-------
U.S. Environmental Protection Agency
Hazard Characterization Document
4. Hazard to the Environment
September, 2014
A summary of aquatic toxicity data submitted for SIDS endpoints is provided in Table 4. The
table also indicates where data for the sponsored substance is read-across (RA) from the
supporting chemical.
Acute Toxicity to Fish
Triethanolamine (CASRN102-71-6, Supporting Chemical)
Fathead minnow (Pimephalespromelas) were exposed to the supporting chemical, TEA for 96
hours under flow-through conditions. Temperature was 23.7 °C, DO = 7.3 mg/L, and pH = 7.8.
Affected fish lost schooling behavior, were hypoactive and darkly colored, had increased
respiration and lost equilibrium prior to death. No other information was provided.
96-hour ECso = 1,180 mg/L (95% C.I. ± 1,060 - 1,300 mg/L)
Acute Toxicity to Aquatic Invertebrates
Triethanolamine (CASRN 102-71-6, Supporting Chemical)
Cladocerans (Ceriodaphnia dubia; 5 per replicate, 3 replicates per test concentration) were
exposed to the supporting chemical, TEA for 48 hours under static conditions to 5 test
concentrations and a control. No other details were provided.
48-hour ECso = 610 mg/L (95% C.I. ± 565.2 - 658.3)
Toxicity to Aquatic Plants
No adequate data
Conclusion: The 96-h LCso value for fish exposed to CASRN 68953-70-8 is 1,180 mg/L based
on the supporting chemical, TEA. The 48-h ECso value for aquatic invertebrates exposed to
CASRN 68953-70-8 is 610 mg/L based on the supporting chemical TEA. There are no adequate
data to assess the toxicity to aquatic plants.
12

-------
U.S. Environmental Protection Agency	September, 2014
Hazard Characterization Document
Table 4. Summary of the Screening Information Data Set
as Submitted under the U.S. HPV Challenge Program -
Aquatic Toxicity Data
Endpoints
SPONSORED CHEMICAL
Oxirane, reaction products with
ammonia, distillation residues
(68953-70-8)
SUPPORTING CHEMICAL
Triethanolamine (TEA)
(102-71-6)
Fish
96-h LCso (mg/L)
(RA)
1,180
1,180
Aquatic Invertebrates
48-h ECso (mg/L)
(RA)
610
610
Aquatic Plants
72-h ECso
growth rate (mg/L)
biomass (mg/L)
No adequate data
Bold = measured data (i.e. derived from testing); RA = read-across
5. References
Warne, M. St. J. and Schifko, A.D. 1999. Toxicity of laundry detergent components to a
freshwater cladoceran and their contribution to detergent toxicity. Ecotoxicology and
Environmental Safety. 44(2): 196-206.
Geiger, D.L., Brooke, L.T., and Call, D.J. 1990. Acute Toxicities of Organic Chemicals to
Fathead Minnows (Pimephales promelas), Volume 5, Center for Lake Superior Environmental
Studies, University of Wisconsin, Superior, WI: 332 p.
13

-------