U.S. Environmental Protection Agency
Hazard Characterization Document
September, 2014
SCREENING-LEVEL HAZARD CHARACTERIZATION
SPONSORED CHEMICAL
Reaction product (cyclododecanol/cyclododecanone/nitric acid) high-boiling
fraction (also known as Corfree®Ml) CASRN 72162-23-3
The High Production Volume (HPV) Challenge Program1 was conceived as a voluntary initiative
aimed at developing and making publicly available screening-level health and environmental
effects information on chemicals manufactured in or imported into the United States in quantities
greater than one million pounds per year. In the Challenge Program, producers and importers of
HPV chemicals voluntarily sponsored chemicals; sponsorship entailed the identification and
initial assessment of the adequacy of existing toxicity data/information, conducting new testing if
adequate data did not exist, and making both new and existing data and information available to
the public. Each complete data submission contains data on 18 internationally agreed to "SIDS"
(Screening Information Data Setl1,2) endpoints that are screening-level indicators of potential
hazards (toxicity) for humans or the environment.
The Environmental Protection Agency's Office of Pollution Prevention and Toxics (OPPT) is
evaluating the data submitted in the HPV Challenge Program on approximately 1400 sponsored
chemicals by developing hazard characterizations (HCs). These HCs consist of an evaluation of
the quality and completeness of the data set provided in the Challenge Program submissions.
They are not intended to be definitive statements regarding the possibility of unreasonable risk of
injury to health or the environment.
The evaluation is performed according to established EPA guidance2'3 and is based primarily on
hazard data provided by sponsors; however, in preparing the hazard characterization, EPA
considered its own comments and public comments on the original submission as well as the
sponsor's responses to comments and revisions made to the submission. In order to determine
whether any new hazard information was developed since the time of the HPV submission, a
search of the following databases was made from one year prior to the date of the HPV
Challenge submission to the present: (ChemID to locate available data sources including
Medline/PubMed, Toxline, HSDB, IRIS, NTP, AT SDR, IARC, EXTOXNET, EPA SRS, etc.),
STN/CAS online databases (Registry file for locators, ChemAbs for toxicology data, RTECS,
Merck, etc.), Science Direct and ECHA.4 OPPT's focus on these specific sources is based on
their being of high quality, highly relevant to hazard characterization, and publicly available.
1 U.S. EPA. High Production Volume (HPV) Challenge Program; http://www.epa.gov/chemrtk/index.htm.
2 U.S. EPA. HPV Challenge Program - Information Sources; http://www.epa.gov/chemrtk/pubs/general/guidocs.htm.
3 U.S. EPA. Risk Assessment Guidelines; http://cfpub.epa.gov/ncea/raf/rafguid.cfm.
4European Chemicals Agency, http://echa.europa.eu
SUPPORTING CHEMICALS
Dodecanedioic acid
Sebacic acid
Undecanedioic acid
CASRN 693-23-2
CASRN 111-20-6
CASRN 1852-04-6
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OPPT does not develop HCs for those HPV chemicals which have already been assessed
internationally through the the Cooperative Chemicals Assessment Program [formerly the HPV
program] of the Organization for Economic Cooperation and Development (OECD) and for
which Screening Initial Data Set (SIDS) Initial Assessment Reports (SIAR) and SIDS Initial
Assessment Profiles (SIAP) are available. These documents are presented in an international
forum that involves review and endorsement by governmental authorities around the world.
OPPT is an active participant in these meetings and accepts these documents as reliable
screening-level hazard assessments.
These hazard characterizations are technical documents intended to inform subsequent decisions
and actions by OPPT. Accordingly, the documents are not written with the goal of informing the
general public. However, they do provide a vehicle for public access to a concise assessment of
the raw technical data on HPV chemicals and provide information previously not readily
available to the public.
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Chemical Abstract
Service Registry
Number
(CASRN)
SPONSORED CHEMICAL
72162-23-3
SUPPORTING CHEMICAL
693-23-2
111-20-6
1852-04-6
Chemical Abstract Index
Name
SPONSORED CHEMICAL
Nitric acid, reaction products with cyclododecanol and
cyclododecanone, by-products from, high-boiling fraction5
SUPPORTING CHEMICALS
Dodecanedioic acid
Decanedioic acid
Undecanedioic acid
Structural Formula
SPONSORED CHEMICAL6'7
0=C(0)CCCCCCCCCCC(=0)0 0=C(0)CCCCCCCCCC(=0)0 0=C(0)CCCCCCCCC(=0)0
Summary
Nitric acid, high boiling reaction products is a mixture of dibasic acids, primarily
dodecanedioic acid, undecanedioic acid and sebacic acid. This mixture is a solid substance
with low vapor pressure and moderate water solubility. The substances of this mixture are
expected to possess moderate mobility in soil. Volatilization is considered low since the main
components of the mixture are expected to exist as anions under environmental conditions and
anions do not volatilize. The rate of hydrolysis is considered negligible for the components of
this mixture. The rate of atmospheric photooxidation is considered moderate for the
components of this mixture. Nitric acid, high boiling reaction products is considered to be
readily biodegradable. Nitric acid, high boiling reaction products is expected to have low
persistence (PI) and low bioaccumulation potential (Bl).
Acute oral and dermal toxicity of nitric acid, high boiling reaction products is low in rats and
rabbits, respectively. No data were available on nitric acid, high boiling reaction products for
repeated dose, developmental or reproductive toxicity. In a combined oral repeated-dose/
reproductive/developmental toxicity study for the supporting chemical dodecanedioic acid,
decreased lymphocyte and leukocyte counts in parental males were seen at > 500 mg/kg-day,
with a parental systemic NOAEL of 100 mg/kg-day. No adverse effects on reproductive or
developmental toxicity parameters were observed. The NOAEL for
5 The term nitric acid high-boiling reaction products will be used as the name for this mixture throughout this document.
6 Additional components are also included in the mixture (at less than 20%).
7This substance is a mixture of dibasic acids, primarily dodecanedioic acid (CASRN 693-23-2) (38-49%) and undecanedioic acid
(CASRN 1852-04-6) (31-38%). The mixture also includes sebacic acid (CASRN 111-20-6) {5-1%), other dibasic acids (9-19%),
other organics (7-11%), nitrogen (0.5%), and water (0.3%).
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reproductive/developmental toxicity is 1000 mg/kg-day (highest dose tested). Nitric acid, high
boiling reaction products did not induce mutations in bacteria in vitro. No data were available
on the clastogenicity of nitric acid high boiling reaction products; however, the supporting
chemical, dodecanedioic acid, did not induce micronuclei in an in vivo mouse micronucleus
test. Nitric acid, high boiling reaction products is irritating to rabbit skin and eyes.
The evaluation of available aquatic toxicity data for acute toxicity of nitric acid, high boiling
reaction products to invertebrates, acute toxicity of the supporting chemical, dodecanedioic
acid, to fish, and toxicity of the supporting chemical, dodecanedioic acid, to aquatic plants,
together with ECOSAR predictions, indicates that the potential hazard of nitric acid, high
boiling reaction products to aquatic organisms is > 100 mg/L.
No data gaps were identified under the HPV Challenge Program.
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Hazard Characterization Document
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The sponsor, E.I. du Pont de Nemours and Company, Inc., submitted a Test Plan and Robust
Summaries to EPA for reaction product (cyclododecanol/cyclododecanone/nitric acid), high-
boiling fraction, also known as Corfree®Ml (CASRN 72162-23-3; CA Index name: nitric acid,
reaction products with cyclododecanol and cyclododecanone, by-products from, high-boiling
fraction) on December 19, 2002. EPA posted the submission on the ChemRTK HPV Challenge
website on January 2, 2003
(http://www.epa.gov/oppt/chemrtk/pubs/summaries/corfree/cl4217tc.htm). EPA comments on
the original submission were posted to the website on July 2, 2003. Public comments were also
received and posted to the website. The sponsor submitted updated/revised documents on
November 19, 2003, which were posted to the ChemRTK website on January 13, 2004.
Supporting Chemical Justification
The sponsor provided data for a supporting chemical, dodecanedioic acid (CASRN 693-23-2).
Dodecanedioic acid is the largest component of nitric acid, high-boiling reaction products and
EPA expects it to have similar physicochemical, environmental and toxicological properties to
those for the other major components. Two additional components of the mixture, sebacic acid
(CASRN 111-20-6) and undecanedioic acid (CASRN 1852-04-6), were used as supporting
substances for physical-chemical and environmental fate properties.
In addition to data provided by the industry sponsor for dodecanedioic acid, an assessment of this
compound is available through the OECD Cooperative Chemicals Assessment Program
(http ://www. oecd. org / env/hazard/data).
1. Chemical Identity
1.1 Identification and Purity
Nitric acid, high boiling reaction products is a white, solid substance. In the submitter's revised
test plan, the components and percentages were described as dodecanedioic acid (-38-49%),
undecanedioic acid (-31-38%), sebacic acid (—5-7%), other dibasic acids (—9-19%), other
organics (-7-11%), nitrogen (—0.5%) and water (—0.3%).
1.2 Physical-Chemical Properties
The physical-chemical properties of this compound are summarized in Table 1. Nitric acid, high
boiling reaction products is a mixture of dibasic acids, primarily dodecanedioic acid,
undecanedioic acid and sebacic acid. This mixture has low vapor pressure and moderate water
solubility.
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Table 1. Physical-Chemical Properties of Nitric Acid,
High Boiling Reaction Products'
Property
Value
CASRN
72162-23-3
Molecular Weight
215 (typical)
Physical State
Solid white odorless flakes
Melting Point
85 - 95 °C (measured)
Boiling Point
250 at 48 mm Hg (measured)2;
294.5 °C at 100 mmHg (measured)3,4;
>300 °C at 760 mmHg (estimated)5
Vapor Pressure
21 mm Hg at 222 °C (measured)2;
1.4 xio 6 mm Hg at 25 °C (estimated)2,5;
5.55 xlO"8 mmHg at 25 °C (measured)3,4
Dissociation Constant (pKa)
4.48 - 4.7 (estimated)
Henry's Law Constant
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2.2 Environmental Exposure and Fate
The environmental fate properties of this compound are provided in Table 2. The components of
nitric acid, high boiling reaction products are expected to have moderate mobility in soil. A
commercial mixture of this substance degraded 63% after 28 days using a CO2 evolution test and
was considered readily biodegradable. Dodecanedioic acid was degraded 71% after 28 days
using the closed bottle (OECD 301D) and was considered readily biodegradable. In addition,
sebacic acid achieved 89 % of its theoretical biochemical oxygen demand (BOD) in 28 days
using the modified MITI (OECD 301 C) test and was readily biodegradable. Volatilization is
considered low since the main components of the mixture are expected to exist as anions under
environmental conditions and anions do not volatilize. The rate of hydrolysis is considered
negligible for the components of this mixture. The rate of atmospheric photooxidation is
considered moderate for the components of this mixture. Nitric acid, high boiling reaction
products is expected to have low persistence (PI) and low bioaccumulation potential (Bl).
Table 2. Environmental Fate Properties of Nitric Acid,
High Boiling Reaction Products'
Property
Value
Photodegradation Half-life
9.1-11.4 hours (estimated)2,3,4,5
Hydrolysis Half-life
Stable
Biodegradation
63% after 28 days (readily biodegradable)
71% after 28 days (readily biodegradable)1,3
89% after 28 days (readily biodegradable)4,6
Bioaccumulation Factor
BAF = 15.1 - 102.9 (estimated)2,3,4,5
Log Koc
2.4 - 2.9 (estimated)2,3,4,5
Fugacity
(Level III Model)2'3,4,5
Air (%)
Water (%)
Soil (%)
Sediment (%)
<0.1
18.4-19.1
80.8-81.2
0.2-0.4
Persistence7
PI (low)
Bioaccumulation7
Bl (low)
'E.I DuPont de Ne Nemours Corporation. November 19, 2003. Revised Test Plan and Robust Summary for
Corfree Ml. Available online at http://www.epa.gov/chemrtk/pubs/summaries/corfree/cl4217tc.htm as of
December 7, 2011.
2U.S. EPA. 2011. Estimation Programs Interface Suite™ for Microsoft® Windows, v4.10. U.S.
Environmental Protection Agency, Washington, DC, USA. Available online at
http://www.epa.gov/opptintr/exposure/pubs/episuitedl.htm as of December 7, 2011.
3 Value for dodecanedioic acid (CASRN 693-23-2)
4 Value for sebacic acid (CASRN 111-20-6)
5 Value for undecanedioic acid (CASRN 1852-04-6)
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6 National Institute of Technology and Evaluation. 2002. Biodegradation and Bioaccumulation of the Existing
Chemical Substances under the Chemical Substances Control Law.
http://www.safe.nite.go.ip/english/kizon/KIZON start hazkizon.html.
'Federal Register. 1999. Category for Persistent, Bioaccumulative, and Toxic New Chemical Substances.
Federal Register 64, Number 213 (November 4, 1999) pp. 60194-60204.
Conclusion: Nitric acid, high boiling reaction products is a mixture of dibasic acids, primarily
dodecanedioic acid, undecanedioic acid and sebacic acid. This mixture is a solid substance with
low vapor pressure and moderate water solubility. The substances of this mixture are expected
to possess moderate mobility in soil. Volatilization is considered low since the main components
of the mixture are expected to exist as anions under environmental conditions and anions do not
volatilize. The rate of hydrolysis is considered negligible for the components of this mixture.
The rate of atmospheric photooxidation is considered moderate for the components of this
mixture. Nitric acid, high boiling reaction products is expected to have low persistence (PI) and
low bioaccumulation potential (Bl).
3. Human Health Hazard
A summary of the human health toxicity data for SIDS and other endpoints is provided in Table
3. The table also indicates where data for the supporting chemical(s) are read-across (RA) to the
sponsored substance, as appropriate.
Acute Oral Toxicity
Nitric acid, high boiling reaction products (CASRN 72162-23-3)
Crl:CD(SD)IGS BR rats (5/sex/dose) were given a single oral gavage dose of 5000 mg/kg and
observed for 14 days after dosing. No deaths occurred during the study.
LDso > 5000 mg/kg
Acute Inhalation Toxicity
Dodecanedioic acid (CASRN 693-23-2, supporting chemical)
Rats (6/group; strain not specified) were exposed to single nose-only test substance
concentrations of 0.81 or 4.3 mg/L for 4 hours. The rats were observed for 14 days,
was observed in this study.
LC50 > 4.3 mg/L
Acute Dermal Toxicity
Nitric acid, high boiling reaction products (CASRN 72162-23-3)
New Zealand white rabbits (5/sex/dose) were administered a single 2000 mg/kg dose to occluded
shaved, intact skin for 24 hours, after which time the test substance was removed. Animals were
(dust)
No mortality
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observed on the day of dosing and during a 14-day observation period. No deaths were observed
during the study.
LD50 > 2000 mg/kg
Repeated-Dose Toxicity
Dodecanedioic acid (CASRN 693-23-2, supporting chemical)
In a repeated-dose reproductive/developmental toxicity screening test, male and female Crl:CD
BR rats (12/sex/dose) were administered daily oral gavage doses of 0, 100, 500 or 1000 mg/kg-
day. After 14 days of dosing, the rats were bred and allowed to produce litters. The test
substance was administered continuously to males and females during breeding, gestation and
lactation (approximately 50 days). After litter production, all adult rats were sacrificed for gross
pathological evaluation. Liver, kidney, adrenals, brain, heart spleen, testes and ovaries were
collected for histopathological examination and weights of liver, kidney, thymus, testes and
epididymides were measured. There were no mortalities. The test substance did not
significantly affect the overall body weights, body weight gains, food consumption or food
efficiency in male or female rats. Some isolated, transient cases of hypoactivity were observed
shortly after dosing in the 500 and 1000 mg/kg-day males and 1000 mg/kg-day females. There
were no significant differences with respect to the reproductive performance of male or female
rats. Mean leukocyte counts were decreased in 500 and 1000 mg/kg-day males, significant only
at 1000 mg/kg-day. The decreases in total leukocyte count were attributable to decreases in
lymphocytes counts, significant in the 500 and 1000 mg/kg-day groups. These changes were
determined to be minimal based on an absence of morphological alterations in the spleen, lack of
decreased thymus weights, and normal serum globulin concentrations. There were no compound
related effects on organ weights, or any gross or microscopic changes noted.
LOAEL (systemic toxicity) = 500 mg/kg-day (based on decreased lymphocyte and leukocyte
counts in males)
NOAEL (systemic toxicity) = 100 mg/kg-day
Reproductive/Developmental Toxicity
Dodecanedioic acid (CASRN 693-23-2, supporting chemical)
In the repeated dose reproductive/developmental toxicity screening test described previously,
assessments of offspring on day 4 postpartum included pup counts, weights and external
appearance. No pathological examination was conducted. There were no significant differences
in reproductive performance of male or female rats or other reproductive or developmental
parameters including clinical observations in pups or pup body weights.
NOAEL (reproductive/developmental toxicity) = 1000 mg/kg-day (highest dose tested)
Genetic Toxicity - Gene Mutations
In vitro
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Nitric acid, high boiling reaction products (CASRN 72162-23-3)
Salmonella typhimurium strains TA98, TA100, TA1535 and TA1537 and Escherichia coli strain
WP2 uvrA were exposed in the presence and absence of metabolic activation to concentrations
of 0, 100, 333, 1000, 3333 or 5000 |ig/plate in the initial assay and 0, 75, 200, 600, 1800 or 5000
|ig/plate in the independent repeat assay. Precipitate and toxicity were not observed in the initial
or independent repeat assay. There was no indication if the positive controls produced an
appropriate response.
Nitric acid, high boiling reaction products was not mutagenic in this assay.
Genetic Toxicity - Chromosomal Aberrations
In vivo
Dodecanedioic acid (CASRN 693-23-2, supporting chemical)
Crl:CD-l(ICR)BR mice (10/sex/concentration) were administered 0, 1000, 2000 or 5000 mg/kg-
bw dodecanedioic acid twice (24 hours apart) by oral gavage. Groups of five male and five
female mice from the negative control and test substance groups were sacrificed 24 and 48 hours
after final dosing. Bone marrow was removed and slides were prepared for examination. The
positive control group (5/sex) was treated with 20 mg/kg cyclophosphamide on 2 consecutive
days and sacrificed 24 hours after the 2nd dose. No significant changes in body weight were
observed in any test substance-treated group. Several animals in the negative and test substance
group exhibited ruffled fur during the study. No statistically significant increases in the
frequency of micronucleated polychromatic erythrocytes were found in test substance treated
animals at any sampling time. No significant decrease in the ratio of young polychromatic
erythrocytes to mature normochromatic erythrocytes was observed. Thus, it is not clear whether
the test substance reached the bone marrow.
Dodecanedioic acid did not induce micronuclei in this assay.
Additional Information
Skin Irritation
Nitric acid, high boiling reaction products (CASRN 72162-23-3)
One female and five male New Zealand white rabbits were subjected to a dermal irritation test.
One rabbit was treated dermally with the test substance 1 day prior to the other five rabbits to
ensure the test substance was neither corrosive nor a severe skin irritant. Approximately 0.5 g of
the test substance was applied to shaved, intact skin of each rabbit and covered with a semi-
occlusive dressing. After a 4-hour exposure period, the dressings were removed. One, 24, 48
and 72 hours after removal of the test substance, the test sites were evaluated for erythema,
edema and other evidence of dermal effects and were scored according to the Draize scale.
Adjacent areas of skin were untreated to serve as the controls. Nitric acid, high-boiling reaction
products was a moderate skin irritant. One rabbit showed no signs of dermal irritation during the
study. The test substance produced no to mild erythema by 1 and 24 hours after test substance
removal. By 48 and 72 hours, all rabbits except one were clear of all irritation; the last rabbit
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exhibited moderate erythema. No edema, clinical signs of toxicity or significant body weight
losses were observed during the study.
Nitric acid, high-boiling reaction products is moderately irritating to rabbit skin.
Eye Irritation
Nitric acid, high boiling reaction products (CASRN 72162-23-3)
Approximately 0.01 g of the test substance was instilled into the lower conjunctival sac of the
right eye of each of 2 male rabbits. Twenty seconds after instillation, the treated and control
eyes of one rabbit were washed. The other rabbit's eyes were not washed. The eyes were
examined on the day of treatment and on days 1, 2, 3 and 7 following treatment. Corfree®Ml
was a moderate eye irritant. The test substance produced moderate conjunctival redness and
moderate discharge in both treated rabbit eyes. Slight chemosis was observed in the treated
unwashed eye, and mild chemosis and blistering on the conjunctiva and nictitating membrane
were observed in the eye washed after treatment. Both treated eyes were normal by 7 days after
administration of the test substance. No clinical signs of toxicity or body weight losses were
observed during the study.
Nitric acid, high-boiling reaction products is moderately irritating to rabbit eyes.
Conclusion: Acute oral and dermal toxicity of nitric acid, high boiling reaction products is low
in rats and rabbits, respectively. No data were available on nitric acid, high boiling reaction
products for repeated dose, developmental or reproductive toxicity. In a combined oral repeated-
dose/ reproductive/developmental toxicity study for the supporting chemical dodecanedioic acid,
decreased lymphocyte and leukocyte counts in parental males were seen at > 500 mg/kg-day,
with a parental systemic NOAEL of 100 mg/kg-day. No adverse effects on reproductive or
developmental toxicity parameters were observed. The NOAEL for reproductive/developmental
toxicity is 1000 mg/kg-day (highest dose tested). Nitric acid, high boiling reaction products did
not induce mutations in bacteria in vitro. No data were available on the clastogenicity of nitric
acid high boiling reaction products; however, the supporting chemical, dodecanedioic acid, did
not induce micronuclei in an in vivo mouse micronucleus test. Nitric acid, high boiling reaction
products is irritating to rabbit skin and eyes.
Table 3. Summary Table of the Screening Information Data Set under the U.S. HPV
Challenge Program - Human Health Data
Endpoint
SPONSORED CHEMICAL
Nitric Acid,
High Boiling Reaction Products
(72162-23-3)
SUPPORTING CHEMICAL
Dodecanedioic Acid
(693-23-2)
Acute Oral Toxicity
LDso (mg/kg)
>5000
> 17,000
Acute Inhalation Toxicity
LCso (mg/L)
No Data
>4.3
(RA)
> 4.3 (4-h)
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Table 3. Summary Table of the Screening Information Data Set under the U.S. HPV
Challenge Program - Human Health Data
Endpoint
SPONSORED CHEMICAL
Nitric Acid,
High Boiling Reaction Products
(72162-23-3)
SUPPORTING CHEMICAL
Dodecanedioic Acid
(693-23-2)
Acute Dermal Toxicity
LDso (mg/kg-bw)
>2000
>6000
Repeated-Dose Toxicity
NOAEL/LOAEL
Oral (mg/kg-day)
No Data
LOAEL = 500
NOAEL = 100
(RA)
LOAEL = 500
NOAEL = 100
Reproductive Toxicity
NOAEL/LOAEL
Oral (mg/kg-day)
Reproductive
No Data
NOAEL = 1000
(RA)
NOAEL = 1000
(highest dose tested)
Developmental Toxicity
NOAEL/LOAEL
Oral (mg/kg-day)
Maternal
No Data
LOAEL = 500
NOAEL = 100
LOAEL = 500
NOAEL = 100
Developmental
NOAEL = 1000
(RA)
NOAEL = 1000
(highest dose tested)
Genetic Toxicity -
Gene Mutations
In vitro
Negative
—
Genetic Toxicity -
Chromosomal Aberrations
In vivo
No Data
Negative
(RA)
Negative
Additional Information
Skin Irritation
Eye Irritation
Irritating
Irritating
-
Measured data in bold; - indicates endpoint not assessed for this chemical; (RA) = Read across
4. Hazard to the Environment
A summary of aquatic toxicity data submitted for SIDS endpoints is provided in Table 4. The
table also indicates where data for the supporting chemical(s) are read-across (RA) to the
sponsored substance, as appropriate.
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Acute Toxicity to Fish
Dodecanedioic acid (CASRN 693-23-2, supporting chemical)
(1) Golden orfe were exposed to nominal concentrations of 200, 500 or 1000 mg/L of the sodium
salt of the test substance for 48 hours. No effects were seen at the maximum concentration. No
additional details were reported.
96-h LCso > 1000 mg/L
(2) ECOSAR was used to estimate toxicity to freshwater fish.
96-h LCso = 136 mg/L
Acute Toxicity to Aquatic Invertebrates
Nitric acid, high boiling reaction products (CASRN 72162-23-3)
Exposure of Daphnia magna (20/group) to nominal concentrations of 0, 7.5, 15, 30, 60 or 120
mg/L of the test substance in an unaerated, static test resulted in 0, 0, 5, 0, 0 and 0% immobility,
respectively, at the end of 48 hours.
48-h ECso> 120 mg/L
Dodecanedioic acid (CASRN 693-23-2, supporting chemical)
(1) Daphnia magna were exposed for 24 hours. No other details were provided.
24-h ECso >27.6 mg/L
(2) ECOSAR was used to estimate the toxicity of daphnids.
48-h ECso = 158 mg/L
Toxicity to Aquatic Plants
Dodecanedioic acid (CASRN 693-23-2, supporting chemical)
(1) Scenedesmus subspicatus were exposed for 72 hours. No other details were provided.
72-h NOEC (growth) > 5.8 mg/L
(2) Scenedesmus subspicatus were exposed for 72 hours in an assimilation test. No other details
were provided.
72-h NOEC (inhibition of oxygen release) > 15.3 mg/L
(3) ECOSAR was used to estimate toxicity to green algae.
96-h ECso = 105 mg/L
Conclusion: The evaluation of available aquatic toxicity data for acute toxicity of nitric acid,
high boiling reaction products to invertebrates, acute toxicity of the supporting chemical,
dodecanedioic acid, to fish, and toxicity of the supporting chemical, dodecanedioic acid, to
aquatic plants, together with ECOSAR predictions, indicates that the potential hazard of nitric
acid, high boiling reaction products to aquatic organisms is > 100 mg/L.
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Table 4. Summary of the Screening Information Data Set under the U.S. HPV Challenge
Program
Aquatic Toxicity Data
Endpoint
SPONSORED CHEMICAL
Nitric Acid, High Boiling
Reaction Products
(72162-23-3)
SUPPORTING CHEMICAL
Dodecanedioic Acid
(693-23-2)
Fish
96-h LCso (mg/L)
No Data
>1000
(RA)
>100
136 (e)
Aquatic Invertebrates
48-h ECso(mg/L)
>120
No Adequate Data
158 (e)
Aquatic Plants
96-h ECso (mg/L)
No Data
123
(RA)
No Adequate Data
123 (e)
Bold = measured data (i.e., derived from testing); e = estimated data using ECOSAR (v. 1.11)
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