vvEPA United States Environmental Protection Agency OSSSS9 Office of Research arid Development National Human Exposure Assessment Survey (NHEXAS) Arizona Study Quality Systems and Implementation Plan for Human Exposure Assessment Title: Verification and Transfer of Data to University of Arizona Source: The University of Arizona Notice: The U.S. Environmental Protection Agency (EPA), through its Office of Research and Development (ORD), partially funded and collaborated in the research described here. This protocol is part of the Quality Systems Implementation Plan (QSIP) that was reviewed by the EPA and approved for use in this demonstration/scoping study. Mention of trade names or commercial products does not constitute endorsement or recommendation by EPA for use. The University of Arizona Tucson, Arizona 85721 Cooperative Agreement CR 821560 Standard Operating Procedure SOP-BCO-G-3.1 U.S. Environmental Protection Agency Office of Research and Development Human Exposure & Atmospheric Sciences Division Human Exposure Research Branch ------- SOP #BCO-G-3.1 Revision# 1 July 10,1997 Page 1 of 25 Verification and Transfer of Data to University of Arizona 1.0 Purpose and Applicability This standard operating procedure (SOP) describes the process of entering analytical data into the analytical results database. This database consists of separate modules for each class of target compounds (pesticides, VOCs, metals, PAH) found in each sampling medium, e.g., metals in carpet dust, pesticides in indoor air, etc. 2.0 Definitions 2.1 OVM 3500 Organic Vapor Monitor: a passive sampler that collects volatile organic compounds (VOCs), such as benzene, toluene, trichloroethene, etc., based on the principal of diffusion. 2.2 Pumped Multisorbent Carbotrap Tube: an active sampler used to collect VOCs onto a sorbent tube. 2.3 Magnetic Storage Medium: any type of diskette, cartridge, tape, or fixed disk used to store computer data. 2.4 Microsoft Access 97 (or later): software program used to create relational computer databases. 2.5 Database: a file or group of files containing records on related data, including ancillary index, report, and query files. 2.6 Laboratory Duplicate First Member (LD1): the first of two aliquots of an environmental sample. Each aliquot is treated identically throughout the laboratory analytical procedure, from preparation through analysis. 2.7 Laboratory Duplicate Second Member (LD2): the second of the two aliquots described above. 2.8 Analytical Duplicate First Member (AD1): the first aliquot of a single environ- mental sample digestate or extract, used to assess analytical instrument precision. 2.9 Analytical Duplicate Second Member (AD2): the second aliquot of the single environmental sample digestate or extract described above. ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 2 of 25 2.10 Laboratory Sample Background for Pre-Preparation Spike (LSO): the first of two aliquots of an environmental sample. This aliquot is analyzed according to the analytical method to establish background concentrations prior to fortification (spiking) with the method analyte(s). 2.11 Laboratory Pre-Preparation Spiked Sample (LSF): the second of the two aliquots described above. This aliquot is subject to fortification (spiking) prior to sample preparation, and measurement(s) of the final concentration(s) are then made according to the analytical method. 2.12 Laboratory Sample Background for Post-Preparation Spike (LPO): an environmental sample exactly like the LSO, except that the aliquot is analyzed according to the analytical method to establish background concentrations prior to fortification (spiking) with the method analyte(s) after all sample preparation has been completed. 2.13 Laboratory Post-Preparation Spiked Sample (LPF): an environmental sample exactly like the LSF, except that the aliquot is fortified (spiked) with the method analyte(s) after all sample preparation has been completed. 2.14 Laboratory Fortified Blank (LFM): an aliquot of reagent water or equivalent neutral reference material, known to be below detection limits for an analyte(s), to which a known quantity(ies) of method analyte(s) was added. The LFM is then treated as an environmental sample in all respects in the laboratory. This includes the addition of all reagents, internal standards, surrogates, glassware, equipment, solvents, and analyses. 2.15 Laboratory Reagent Blank (LRB): an aliquot of reagent water or equivalent neutral reference material treated as an environmental sample in all respects in the laboratory. This includes the addition of all reagents, internal standards, surrogates, glassware, equipment, solvents, and analyses. 2.16 Pre-Shipment Blank (PSB): pre-cleaned, blank sampling medium treated as an environmental sample in all respects in the laboratory. This includes the addition of all reagents, internal standards, and surrogates; and the use of glassware, equipment, and solvents. 2.17 Laboratory Reference Material (LRM): an aliquot of sample having a certified value. These samples are usually obtained from NIST, EPA/EMSL, etc. The LRM is treated as an environmental sample in all respects in the laboratory. This includes the addition of all reagents, internal standards, surrogates, glassware, equipment, solvents, and analyses. ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 3 of 25 2.18 Pre-Shipment Spike (PSS): pre-cleaned sampling medium that is fortified (spiked) with a known amount of the method analyte(s), and treated as an environmental sample in all respects in the laboratory. This includes the addition of all reagents, internal standards, and surrogates; and the use of glassware, solvents, and equipment. 2.19 Field Duplicate First Member (FD1): the first aliquot of a single environmental matrix sampling event. Each aliquot is collected and treated identically throughout the field collection, storage, shipment, preparation and analysis procedures. 2.20 Field Duplicate Second Member (FD2): the second of the two aliquots as described above. 2.21 Field Method Blank (FMB): pre-cleaned, blank sampling medium that is not exposed, but otherwise treated as an environmental sample in all respects during field sample collection, storage, shipment, preparation and analysis procedures. 2.22 Field Method Spike (FMS): pre-cleaned sampling medium that is fortified (spiked) with a known amount of the method analyte(s), not exposed, but otherwise treated as an environmental sample in all respects during field sample collection, storage, shipment, preparation and analysis procedures. 2.23 Arizona Lab Blank (ALB): pre-cleaned, blank sampling medium that is not transported into the field, but otherwise treated as an environmental sample in all respects during any University of Arizona (UA) laboratory procedures. This includes the addition of all reagents, internal standards, and surrogates; and the use of glassware, solvents, and equipment. 2.24 VirusScan for Windows95 Version 3.0 (or higher): a computer virus-scanning program used to detect computer viruses and disinfect PC hard drives and diskettes. Battelle has a site license with McAfee International Associates (www.mcafee.com) and regularly receives upgraded versions of VirusScan. References 3.1 "Microsoft Access for Windows 95 Power Toolkit: Cutting-Edge Tools & Techniques for Programmers," Michael Groh, Ventana, 1996. 3.2 "Office 97 Bible," Edward Jones and Derek Sutton II. IDG Books Worldwide, 1997. ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 4 of 25 3.3 "Running Microsoft Access 2 for Windows," John L. Viescas, Microsoft Press, 1994. 3.4 "Microsoft Excel Version 5.0 User's Guide," Microsoft Corporation, 1993-94. 3.5 "Implementation of EPA Order 2180.2 Standard Format for Media and Record Formats for the National Human Exposure Assessment Survey Pilot Studies," L.J. Barlion; Environmental Monitoring Systems Laboratory, U.S. Environmental Protection Agency, Cincinnati, OH, April 5, 1995. 4.0 Discussion Using a relational database to compute human exposure assessment concentrations provides a consistent, integrated structure for the reporting of analytical results. All equations are validated, and cannot be altered in any way during data entry. Entered numbers can be compared directly with raw data values during the QA/QC proofreading process. All fields are searchable, and complex or simple reports can be generated with a few keystrokes. 5.0 Responsibilities 5.1 Analyst Entering Data into a Project Spreadsheet Template 5.1.1 The analyst receives a validated spreadsheet template (see Figure 1 for an example of a spreadsheet template) for a given pollutant in a given environmental matrix (e.g., "DWP.xlt" is the filename for the Excel spreadsheet template for dermal wipe pesticide results - see Table 1 for a list of monitored environmental matrices and their corresponding Excel template filenames and Access data entry form names) from the database technician. 5.1.2 The analyst enters the information as described in Section 7.0 ("Procedure") of this SOP. 5.1.3 The analyst saves the file as an Excel spreadsheet, using the following filename convention: the first three letters of the file are the sample s visual identifier code (VID); the next four letters of the file are the month and date on which the raw data was collected (mmdd), e.g., DWP0124.xls is a file containing results for dermal wipe pesticide samples analyzed on Jan. 24. Filenames can be appended with letters for cases when it requires ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 5 of 25 more than one file to report the analytical data for a given analysis date (e.g., DWP0124a.xls, DWP0124b.xls, etc.). 5.1.4 The analyst delivers a backup copy of the spreadsheet file and the corresponding raw data and field forms to the database technician. The database technician electronically imports the file into the appropriate project database module using an Access 97 macro (see Figure 2). 5.1.5 The database technician prints out a copy of the report, proofreads the data, and makes any necessary corrections. 5.1.6 The database technician creates a backup copy of the modified database module. 5.1.7 Pending the analyst's review/approval of the corresponding QA/QC data, the database technician packages and ships, or electronically transfers the completed database modules (for a given sample set) to UA. 5.2 Electronically Transferring Data to Project Spreadsheet Template 5.2.1 The analyst electronically transfers the raw data for a given pollutant in a given environmental matrix to an Excel 97 spreadsheet (see Figure 3). 5.2.2 The information is imported into the appropriate project database template using an Excel 97 macro, as described in Section 7.0 ("Procedure'). 5.2.3 The analyst proofreads the data in the template, makes any necessary corrections, and gives the final template and folder containing all relevant information to the database technician. 5.2.4 The database technician imports the final template into the database using an Access 97 macro. The folder is then filed with other project data in a locked file cabinet. 5.2.5 Pending the analyst's review/approval of the corresponding QA/QC data, the database technician packages and ships, or electronically transfers the completed database modules (for a given sample set) to UA. Materials 6.1 Microsoft Access 97 (or later version) ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 6 of 25 6.2 Microsoft Excel 97 (or later version) 6.3 IBM-compatible personal computer, pentium processor or better 6.4 3.5" double-sided, high density IBM-compatible formatted diskettes Procedure 7.1 Results will be electronically imported or entered into an Excel 97 project spreadsheet template by the analyst, using an Excel 97 macro. Then the spreadsheet template will be imported into the appropriate Access 97 project database module, by the database technician using an Access 97 macro. 7.2 Entering Data for Metals from Dermal and Surface Wipes, Floor Dust, and Foundation and Yard Soil. 7.2.1 Open the Excel 97 results template, (e.g., Excel filename: "DWM.xlt," for entering results for dermal wipe metals samples). 7.2.2 Positioning your cursor under the "Sample ID" column, enter the sample identification number for the sample for which you wish to enter results. Tab the cursor past the "Compound" to the "Raw Data" column. Enter the concentrations of each of the target metals (jag/mL) for that sample, taken directly from the raw data calibration curve. 7.2.3 Tab to the "Raw ID" column, and enter the raw data identification code. For metals: "visual identifier code-analysis date", e.g. "FFM-072196" denotes fixed filter metals, analyzed on July 7, 1996. 7.2.4 Tab to the "Det Limit" column, and enter the detection limit (^ig/mL) for each of the target metals for the analyses conducted on that day. 7.2.5 Tab to the "FB ID" column, and enter the sample identification number of the appropriate field blank. 7.2.6 Tab to the "F Blank" column, and enter the concentration of each of the target metals (fig/mL) in the identified field blank. 7.2.7 Tab to the "Diln Fac" column, and enter the dilution factor (unitless) by which the sample result for each metal should be multiplied. If the sample is a dermal wipe, you have completed entering the information necessary to calculate a final concentration for the metals in this sample, and you ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 7 of 25 should continue with the next sample. However, if the sample is a QA/QC sample, proceed to section 7.6 to enter the rest of the information for this sample. 7.2.8 For surface wipe samples only, tab to the "Area 1" column and enter the of the first area wiped (cm2), as indicated on the sample's field form. Tab to the "Area 2" column and enter the second area wiped (cm2), as indicated on the sample's field form. For a QA/QC sample, proceed to section 7.6; otherwise proceed to the next sample. 7.2.9 For floor dust, foundation and yard soil samples only, tab to the "Weight" column and enter the weight of the digested dust or soil aliquot (g). 7.2.10 For floor dust samples only, tab to the "Area" column and enter the area vacuumed (m2). Proceed to section 7.6 for a QA/QC sample; otherwise proceed to the next sample. 7.2.11 For fixed site air samples only, tab to the "Time" column and enter the Actual Pump Run Time (min) for metals, as reported on the field form. 7.2.12 Tab to the "I-Flow" column and enter the Start Flow reading (L/min) measured during air sampler setup and reported on the field form. 7.2.13 Tab to the "F-Flow" column and enter the Stop Flow reading (L/min) measured during air sampler take-down and reported on the field form. 7.2.14 For a QA/QC sample, proceed to section 7.6; otherwise proceed to the next sample. Entering Data for Pesticides from Dermal Wipes, and Floor Dust 7.3.1 Open the Excel 97 results template corresponding to the environmental matrix for which you wish to enter results (e.g., Excel filename: "DWP.xlt," for entering results for dermal wipe pesticides samples). 7.3.2 Position your cursor under the "Sample ID" column, and enter the sample identification number for the sample for which you wish to enter results. Tab the cursor past the "Compound" column to the "Raw Data' column. Enter the concentration of each of the target pesticides (p.g/mL) for that sample, taken directly from the raw data calibration curve. ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 8 of 25 7.3.3 Tab to the "Raw ID" column and enter the raw data identification code listed on the raw data. For pesticides: "Visual identifier-analysis date", e.g. "DWP-071496" denotes dermal wipe pesticides, analyzed on July 14, 1996. 7.3.4 Tab to the "Det Limit" column and enter the detection limit Og/mL) for each of the target pesticides for the analyses conducted on that day. 7.3.5 Tab to the "FB ID" column and enter the sample identification number of the appropriate field blank. 7.3.6 Tab to the "F Blank" column and enter the surrogate recovery-corrected concentration of each of the target pesticides (|j.g/mL) in the identified field blank. 7.3.7 Tab to the "Surr Rec" column and enter the recovery (%) of the surrogate recovery standard for that sample. 7.3.8 Tab to the "Diln Fac" column and enter the dilution factor (unitless) by which the sample result for each of the target pesticides should be multiplied. If the sample is a dermal wipe, you have completed entering the information necessary to calculate a final concentration for the pesticide in this sample and you should continue with the next sample. However, if the sample is a QA/QC sample, proceed to section 7.6 to enter the rest of the information for this sample. 7.3.9 For floor dust samples only, tab to the "Weight" column and enter the weight of the extracted dust or soil aliquot (g). 7.3.10 Tab to the "Area" column and enter the area vacuumed (m2). Proceed to section 7.6 for a QA/QC sample; otherwise proceed to the next sample. Entering Data for OVM 3500 Organic Vapor Monitor and Pumped Multisorbent Carbotrap (200 & 300) Tube Samples. 7.4.1 Open the Excel 97 results template corresponding to the environmental matrix for which you wish to enter results (e.g., Excel filename: "OVM.xlt," Access form: "OVM Results" for entering results for organic vapor monitor samples). 7.4.2 Position your cursor under the "Sample ID" column, and enter the sample identification number for the sample for which you wish to enter results. ------- SOP #BCO-G-3.1 Revision# 1 July 10,1997 Page 9 of 25 Tab the cursor past the "Compound" column to the "Raw Data" column. Enter the amount of each of the VOCs measured (jj.g) for that sample, taken directly from the raw data calibration curve. 7.4.3 Tab to the "Raw ID" column and enter the raw data identification code. For VOCs: "Visual identifier-analysis date", e.g. "OVM-071896" denotes Organic Vapor Monitor, analyzed on July 18, 1996. 7.4.4 Tab to the "Det Limit" column and enter the detection limit (fig) for each of the VOCs for the analyses conducted on that day. 7.4.5 Tab to the "FB ID" column and enter the sample identification number of the appropriate field blank. 7.4.6 Tab to the "F Blank" column and enter the amount of each of the VOCs (|LXg) measured in the identified field blank. 7.4.7 Tab to the "Time" column, and enter the time (min) for which the passive sample was exposed (OVM 3500 organic vapor monitor) or the time (min) for which the pump sampled for VOCs (actively pumped multisorbent Carbotrap tube). If the sample is an OVM 3500 organic vapor monitor sample, you have completed entering the information necessary to calculate a final concentration for the VOC in this sample, and you should continue with the next sample. However, if the sample is a QA/QC sample, proceed to section 7.6 to enter the rest of the information for this sample. 7.4.8 For pumped multisorbent Carbotrap tube samples only: 7.4.8.1 Tab to the "I-Flow" column and enter the Start Flow reading (cm3/min) measured during air sampler setup and reported on the field form. 7.4.8.2 Tab to the "F-Flow" column, and enter the Stop Flow reading (cm3/min) measured during air sampler take-down and reported on the field form. 7.4.8.3 For a QA/QC sample, proceed to section 7.6; otherwise proceed to the next sample. ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 10 of 25 Entering Data for PAH Air Samples 7.5.1 Open the Excel 97 results template corresponding to the environmental matrix for which you wish to enter results (e.g., Excel filename: "PAP.xlt," for entering results for Pesticide/PAH Combination Air Samples). 7.5.2 Position your cursor under the "Sample ID" column, and enter the sample identification number for the sample for which you wish to enter results. Tab the cursor past the "Compound" column to the "Raw Data" column. Enter the concentration of each of the target pesticides (jag/mL) for that sample, taken directly from the raw data calibration curve. 7.5.3 Tab to the "Raw ID" column and enter the raw data identification code listed on the raw data. For pesticides: "Visual identifier-analysis date", e.g. "PAH-071496" denotes active airborne PAH monitor, analyzed on July 14, 1996. 7.5.4 Tab to the "Det Limit" column and enter the detection limit (|ag/mL) for each of the target pesticides for the analyses conducted on that day. 7.5.5 Tab to the "FB ID" column and enter the sample identification number of the appropriate field blank. 7.5.6 Tab to the "F Blank" column and enter the surrogate recovery-corrected concentration of each of the target pesticides (^ig/mL) in the identified field blank. 7.5.7 Tab to the "Diln Fac" column and enter the dilution factor (unitless) by which the sample result for each of the target pesticides should be multiplied. 7.5.8 Tab to the "I-Flow" column and enter the Start Flow reading (L/min) measured during air sampler setup and reported on the field form. 7.5.9 Tab to the "F-Flow" column and enter the Stop Flow reading (L/min) measured during air sampler take down and reported on the field form. 7.5.10 For a QA/QC sample, proceed to section 7.6; otherwise proceed to the next sample. ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 11 of 25 Battelle Laboratory QA/QC Samples 7.6.1 If the sample is a laboratory QC sample, tab to the "QCC" column and enter the applicable 3-digit QC code: QCC Name LDl Laboratory Duplicate First Member LD2 Laboratory Duplicate Second Member AD1 Analytical Duplicate First Member AD 2 Analytical Duplicate Second Member LSO Laboratory Sample (Background for Pre-Preparation Spike) LSF Laboratory Pre-Preparation Spiked Sample LPO Laboratory Sample (Background for Post-Preparation Spike) LPF Laboratory Post-Preparation Spiked Sample LFM Laboratory Fortified Blank LRB Laboratory Reagent Blank PSB Pre-Shipment Blank PSS Pre-Shipment Spike LRM Laboratory Reference Material FD1 Field Duplicate First Member FD2 Field Duplicate Second Member FMB Field Method Blank FMS Field Method Spike ALB Arizona Lab Blank Tab to the "QC Result" column and enter the applicable QC results: QCC OC Result LDl None (default) LD2 Relative Percent Difference (%) AD1 None (default) AD2 Relative Percent Difference (%) LSO None (default) LSF Percent Recovery (%) LPO None (default) LPF Percent Recovery (%) LFM Percent Recovery (%) LRB None (default) PSB None (default) PSS Percent Recovery (%) LRM Percent Recovery (%) ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 12 of 25 FD1 None (default) FD2 Relative Percent Difference (% FMB None (default) FMS Percent Recovery (%) ALB None (default) 7.6.3 Proceed to the next sample. Completion of Data Entry, Archiving Data to Floppy Diskette 7.7.1 Print out a report of your results and proof the data entered. 7.7.2 After making any necessary changes, save the data in a file on the hard drive. 7.7.3 Save a copy of each modified database module on a floppy diskette or magnetic tape backup. 7.7.4 Label the diskette or magnetic tape backup with the filenames, and the date the backup was made. Calculations 7.8.1 The human exposure concentration of a metal in a dermal wipe is expressed as: (R-Bf)*D = (ig where: Default Decimal Value Places R = value obtained from raw data calibration curve (ng/mL) none 3 Bf = field blank ((ig/mL) D = dilution factor (mL) 0.000 3 1.00 2 7.8.2 The human exposure concentration of a metal in a surface wipe is expressed as. ( R - Bf) * D * ( 1 / [ (L /100) * (W /100 ) ] = ng/m2 where: Default Decimal Value Places R = value obtained from raw data calibration curve (fig/mL) none 3 Bf = field blank ((ig/mL) 0.000 3 ------- SOP #BCO-G-3.1 Revision# 1 July 10, 1997 Page 13 of 25 D = dilution factor (mL) L = length of area wiped (cm) W = width of area wiped (cm) 1.00 2 none 1 none 1 7.8.3 The human exposure concentration of a metal in floor dust is expressed as: (R-Bf)*D*(l/W) = ]Ug/g fine dust where: Default Decimal Value Places R = value obtained from raw data calibration curve (|ig/mL) Bf = field blank ((ig/mL) D = dilution factor (mL) W = weight of the dust aliquot (g) none 3 0.000 3 1.00 2 none 2 or: ( R - Bf) * D * { 1 / [ A * (W / Wx) ] } = ng/m2 where: R = value obtained from raw data calibration curve (|ig/mL) Bf = field blank (ng/mL) D = dilution factor (mL) A = area vacuumed (m2) W = weight of the dust aliquot (g) WT = weight of the total dust sample collected (g) Default Decimal Value Places none 0.000 1.00 2.00 none none 3 3 2 2 2 2 7.8.4 The human exposure concentration of a metal in foundation or yard soil is expressed as: (R-Bf)*D*( 1 / W) = |ig/g where: Default Decimal Value Places R = value obtained from raw data calibration curve (|ig/mL) Bf = field blank (|ig/mL) D = dilution factor (mL) W = weight of the dried soil aliquot (g) none 3 0.000 3 1.00 2 none 2 ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 14 of 25 7.8.5 The human exposure concentration of a metal in a fixed site sample is expressed as: [( R - Bf) * D] / { 0.001 * [ Ts * 0.5 * ( F; + Ff) ] } = ng/m3 where: Default Decimal Value Places R = value obtained from raw data calibration curve (|ig/mL) Bf = field blank (jag/mL) D = dilution factor (mL) Ts = actual pump run time (min) F, = start flow (L/min) Ff = stop flow (L/min) none 0.000 1.00 none 4.00 4.00 3 3 2 1 2 2 7.8.6 The human exposure concentration of a pesticide in a dermal wipe is expressed as: { [ R / ( S /100 ) ] - Bf} * D = jag where: Default Decimal Value Places R = value obtained from raw data calibration curve ((ig/mL) S = surrogate recovery (%) Bf = surrogate recovery-corrected field blank (|ig/mL) D = dilution factor (mL) none 100 0.000 1.00 7.8.7 The human exposure concentration of a pesticide in floor dust is expressed as: { [ R / ( S / 100 ) ] - Bf} * D * ( 1 / W ) = jig/g fine dust where: Default Decimal Value Places R = value obtained from raw data calibration curve (|ig/mL) S = surrogate recovery (%) Bf =surrogate recovery-corrected field blank (|ig/mL) D = dilution factor (mL) W = weight of the dust aliquot (g) none 100 0.000 1.00 none or: [ R / ( S / 100 ) ] - Bf} * D * { 1 / [ A * ( W / WT ) ] } = Hg/m2 where: ------- SOP #BCO-G-3.1 Revision# 1 July 10, 1997 Page 15 of 25 Default Decimal Value Places R = value obtained from raw data calibration curve (fig/mL) none 3 S = surrogate recovery (%) 100 0 Bf = surrogate recovery-corrected field blank (|ag/mL) 0.000 3 D = dilution factor (mL) 1.00 2 A = area vacuumed (m2) 2.00 2 W = weight of the dust aliquot (g) none 2 WT = weight of the total dust sample collected (g) none 2 7.8.8 The human exposure concentration of a pesticide in a fixed site air sample is expressed as: ({ [ R / ( S /100 ) ] - Bf} * D ) / { 0.001 * [ Ts * 0.5 * ( Fi + Ff) ] } = ng/m3 where: Default Decimal Value Places R = value obtained from raw data calibration curve (ng/mL) none 0 S = surrogate recovery (%) 100 0 Bf = surrogate recovery-corrected field blank (ng/mL) 0 0 D = dilution factor (mL) 1.0 1 Ts = actual pump run time (min) none 1 Fj = start flow (L/min) 4.00 2 Ff = stop flow (L/min) 4.00 2 7.8.9 The human exposure concentration of a PAH in floor dust is expressed as: [ ( R - B ) * 0.001 ] * D * ( 1 / W ) = jag/g fine dust where: R = value obtained from Quan report (ng/mL) B = field blank value obtained from Quan report (ng/mL) D = dilution factor (mL) W = weight of the dust aliquot (g) Default Decimal Value Places none 0.000 1.00 none 3 3 2 2 or: ( R - B ) * 0.001 ] ~ D * { 1 / [ A * ( W / WT ) ] } = |^g/m2 where: ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 16 of 25 Default Decimal Value Places R = value obtained from Quan report (ng/mL) B = field blank value obtained from Quan report (ng/mL) D = dilution factor (mL) A = area vacuumed (m2) W = weight of the dust aliquot (g) WT = weight of the total dust sample collected (g) none 3 0.000 3 1.00 2 2.00 2 none 2 none 2 7.8.10 The human exposure concentration of a PAH in a fixed site air sample is expressed as: (R-B) * D / { 0.001 * [ Ts * 0.5 * ( F; + Ff) ] } = ng/m3 where: Default Decimal Value Places R = value obtained from Quan report (ng/mL) B = field blank value obtained from Quan report (ng/mL) D = dilution factor (mL) Ts = actual pump run time (min) Fj = start flow (L/min) Ff = stop flow (L/min) none 0 1.0 none 4.00 4.00 7.8.11 The human exposure concentration of a volatile organic compound in a fixed site (passive OVM 3500 badge) air sample is expressed as: (R-Bf) * 106 / K * Cr * T = ng/m3 where: Default Decimal Value Places R = value obtained from raw data calibration curve (fig) Bf = field blank (|J.g) K = sampling rate for compound of interest (cm3/min) Cr = recovery coefficient for compound of interest (unitless) T = time the OVM 3500 was exposed (min) or: [ {( R - Bf) * 106} / K * Cr * T ] * X =ppbv where: none I 0.00 2 none 1 1.00 2 none 1 ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 17 of 25 Default Decimal Value Places R = value obtained from raw data calibration curve (|ig) Bf = field blank (|ig) K = sampling rate for compound of interest (cm3/min) Cr = recovery coefficient for compound of interest (unitless) T = time the OVM 3500 was exposed (min) X = conversion factor for compound of interest (ppbv/|ig/m3) none 0.00 none 1.00 none none 2 2 1 2 1 4 Recovery Compound Sampling Rate Coefficient Conversion Factor (K) (Cr) (X) Benzene 35.5 0.95 0.3130 m-D ichlorobenzene 27.8 0.87 0.1663 p-Dichlorobenzene 27.8 0.87 0.1663 Dichloromethane 37.9 0.97 0.2879 Ethylbenzene 27.3 0.96 0.2303 Styrene 26.8 0.82 0.2348 T etrachloroethylene 31.1 0.95 0.1474 Toluene 31.4 1.00 0.2654 1,1,2-Trichloroethane 29.7 0.95 0.1833 Trichloroethylene 31.1 0.99 0.1861 m-/p-Xylene 27.3 0.97 0.2303 o-Xylene 27.3 0.97 0.2303 .12 The human exposure concentration of a volatile organic compound in a fixed site (pumped miiltisorbent Carbotrap tube') air sample is expressed as: ( R - Bf ) / [( Fj + Ff) * 0.5 * T * 10"3 ] = ng/m3 where: Default Decimal Value Places R = value obtained from raw data calibration curve (ng) Bf = field blank (ng) Fj = start flow (cm3/min) Ff = stop flow (cm3/min) T = actual pump runtime (min) none 0.00 5.0 5.0 none 0 0 1 1 1 or: ( R - Bf) / [( Fj + Ff) / 2 * T * 10"3 ] * X = ppbv where: ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 18 of 25 Default Decimal Value Places R = value obtained from raw data calibration curve (ng) none 0 Bf = field blank (ng) 0.00 0 F; = start flow (cmVmin) 5.0 1 Ff = stop flow (cm3/min) 5.0 1 T = actual pump run time (min) none 1 X = conversion factor for compound of interest (ppbv/(j.g/m3) none 4 Compound Conversion Factor (X) 1,3-butadiene 0.4520 benzene 0.3130 trichloroethene 0.1861 toluene 0.2654 1,1-dichloroethene 0.2522 dichloromethane 0.2879 1,1 -dichloroethane 0.2471 cis-1,2-dichloroethene 0.2522 trichloromethane 0.2048 1,2-dichloroethane 0.2471 1,1,1 -trichloroethane 0.1833 carbon tetrachloride 0.1590 1,2-dichloropropane 0.2164 1,1,2-trichloroethane 0.1833 tetrachloroethene 0.1474 chlorobenzene 0.2172 ethylbenzene 0.2303 m+p-xylene 0.2303 styrene 0.2348 1,1,2,2-tetrachloroethane 0.1457 o-xylene 0.2303 m-dichlorobenzene 0.1663 p-dichlorobenzene 0.1663 o-dichlorobenzene 0.1663 13 Relative percent difference for LD2. AD2. and FD2 is expressed as: {(| C, - C21) / [ ( C, + C2) / 2 ] } * 100 = Relative Percent Difference (%) where: C, = Analyte Cone (ng/mL) in: C2 = Analyte Cone (ng/mL) in: LD1 LD2 AD1 AD2 FD1 £1^ ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 19 of 25 7.8.14 Percent recovery of analvtes in LSF. LPF. LFM. LRM and FMS PC samples is expressed as: [ ( C, - C2) / Cspk ] * 100 = Recovery (%) where: C, = Analyte Cone C2 = Analyte Cone Cspk = Analyte Cone (|ig/mL) in: (|ig/mL) in: (fig/mL) in: LSF LSO Fortification (spike) LPF LPO Fortification (spike) LFM LRB Fortification (spike) LRM LRB Fortification (spike) FMS FMB NIST or EMSL certification 7.9 Quality Control 7.9.1 All hard-coded computation performed within a given database module will be validated before entering the project results into the module. 7.9.2 Calculated fields will not be modifiable by the analyst or database technician. 7.9.3 Upon completing the entries into a given database module, a report will be printed out and hand-entered data will be proofread by a second party. 7.9.4 All magnetic storage media will be scanned for virus infection, using the most current version of VirusScan for Windows95 version 3.0 (or higher) prior to shipment. 7.9.5 All magnetic storage media will be shipped in anti-static, rigid diskette mailers. Records 8.1 Records of all raw data used to compute pollutant concentrations in environmental matrices will be kept in the custody of the analyst for three years after completion of the study. 8.2 Identified and dated magnetic diskette backups of shipped database modules will be archived in read-only format by the database technician for three years after completion of the study. The archives will be stored at room temperature in a clean area, free from strong magnetic fields. ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 20 of 25 NHEXAS DWM G283317-21 DATA 7/10/97 _ __. -— - - — - - - (ug/mL) (ug/mL) (ug/mL) Sample ID Compound Raw Data Raw ID Det Limit FB ID F Blank Diln Fac QCC QC Result barium 0 cadmium 0 0 0 0 chromium 0 0 0 0 copper 0 0 0 0 manganese 0 0 0 0 nickel 0 0 0 0 lead 0 0 0 0 selenium 0 0 0 0 vanadium 0 0 0 0 zinc 0 0 0 Figure 1. Example of an Excel97 spreadsheet template for dermal wipe metals analytical results entry. ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 21 of 25 Access 97 Visual Basic Import Macro for Importing Dermal Wipe Metals Data Option Compare Database Option Explicit ' DWM_Import_Macro Function DWM_Import_Macro() On Error GoTo DWM_Import_Macro_Err ' Import metals data for sample 1 DoCmd.TransferSpreadsheet aclmport, 5, "DWM Results", "c:\lNHXTRACK\im\import.xls", True, "dwmpl" ' Import metals data for sample 2 DoCmd.TransferSpreadsheet aclmport, 5, "DWM Results", "c:\lNHXTRACK\im\import.xls", True, "dwmp2" ' Import metals data for sample 3 DoCmd.TransferSpreadsheet aclmport, 5, "DWM Results", "c:\lNHXTRACK\im\import.xls", True, "dwmp3" ' Import metals data for sample 4 DoCmd.TransferSpreadsheet aclmport, 5, "DWM Results", "c:\lNHXTRACK\im\import.xls , True, "dwmp4" ' Import metals data for sample 5 DoCmd.TransferSpreadsheet aclmport, 5, "DWM Results", "c:\lNHXTRACK\im\import.xls , True, "dwmp5" ' Import metals data for sample 6 DoCmd.TransferSpreadsheet aclmport, 5, "DWM Results", "c:\lNHXTRACK\im\import.xls , True, "dwmp6" ' Import metals data for sample 7 DoCmd.TransferSpreadsheet aclmport, 5, "DWM Results", "c:\lNHXTRACK\im\import.xls , True, "dwmp7" ' Import metals data for sample 8 DoCmd.TransferSpreadsheet aclmport, 5, "DWM Results", "c:\lNHXTRACK\im\import.xls , True, "dwmp8" ' Import metals data for sample 9 Figure 2. Example of Access 97 Visual Basic import macro for importing dermal wipe metals data for each often elements analyzed using inductively couple plasma atomic emission spectroscopy (ICP/AES) for ten individual samples from given analysis date into corresponding database table. ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 22 of 25 DoCmd.TransferSpreadsheet aclmport, 5, "DWM Results", "c:\lNHXTRACK\im\import.xls", True, "dwmp9" ' Import metals data for sample 10 DoCmd.TransferSpreadsheet aclmport, 5, "DWM Results", "c:\lNHXTRACK\im\import.xls", True, "dwmplO" D WM_Import_Macro_Exit: Exit Function DWM_Import_Macro_Err: MsgBox Error$ Resume DWM_Import_Macro_Exit End Function Figure 2. (continued). ------- SOP #BCO-G-3.1 Revision# 1 July 10,1997 Page 23 of 25 Excel 97 Visual Basic import macro for importing pesticide data into NHEXAS spreadsheet templates ' Standards Macro ' Macro recorded 12/31/96 by ASATD1 » ' Keyboard Shortcut: Ctrl+s » Sub Standards() ActiveCell.Select Selection.Copy Windows("GCMSDstd.xlt"). Activate Selection.PasteSpecial Paste:=xlValues, Operation:=xlNone, _ SkipBlanks:=False, Transpose—False Windows("410RERUN.xls"). Activate ActiveWindow.WindowState = xlNormal ActiveCell.Offset(0, l).Range("Al").Select Application. CutCopyMode = False Selection.Copy Windows("GCMSDstd.xlt"). Activate ActiveCell.Offset(0, 3).Range("A1'").Select Selection.PasteSpecial Paste:=xlValues, Operation:=xlNone, _ SkipBlanks:=False, Transpose:=False Windows("410RERUN.xls"). Activate ActiveWindow.WindowState = xlNormal ActiveCell.Offset(0, l).Range("Al").Select Application. CutCopyMode = False Selection.Copy Windows("GCMSDstd.xlt"). Activate ActiveCell.Offset(0, 2).Range("A1 ").Select Selection.PasteSpecial Paste:=xlValues, Operation:=xlNone, _ SkipBlanks:=False, Transpose—False Windows("410RERUN.xls"). Activate ActiveWindow.WindowState = xlNormal ActiveCell.Offset(0, l).Range("Al").Select Application.CutCopyMode = False Selection.Copy Windows("GCMSDstd.xlt"). Activate Figure 3. Example of Excel 97 Visual Basic import macro for importing pesticide data into NHEXAS spreadsheet templates. ------- ActiveCell.Offset(0, 2).Range("A1 ").Select Selection.PasteSpecial Paste:=xlValues, Operation:=xlNone, SkipBlanks:=False, Transpose:=False Windows("410RERUN.xls"). Activate ActiveWindow.WindowState = xlNormal ActiveCell.Offset(0, l).Range("Al").Select Application.CutCopyMode = False Selection.Copy Windows("GCMSDstd.xlt"). Activate ActiveCell.Offset(0, 2).Range("A1").Select Selection.PasteSpecial Paste:=xlValues, Operation:=xlNone, SkipB lanks :=False, Transpose:=False Windows("410RERUN.xls"). Activate ActiveWindow.WindowState = xlNormal ActiveCell.Offset(0, l).Range("Al").Select Application.CutCopyMode = False Selection.Copy Windows("GCMSDstd.xlt"). Activate ActiveWindow.SmallScroll ToRight:=2 ActiveCell.Offset(0, 2).Range(*'Al").Select Selection.PasteSpecial Paste~xlValues, Operation:=xlNone, SkipB lanks :=False, Transpose :=False Windows("410RERUN.xls"). Activate ActiveWindow.WindowState = xlNormal ActiveWindow.SmallScroll ToRight:=3 ActiveCell.Offset(0, l).Range("Al:Ll").Select Application.CutCopyMode = False Selection.Copy Windows("GCMSDstd.xlt"). Activate ActiveWindow.ScrollColumn = 1 ActiveCell.Offset(7, -1 l).Range("Al").Select Selection.PasteSpecial Paste:=xlValues, Operation:=xlNone, SkipBlanks:=False, Transpose:=False Windows("410RERUN.xls").Activate ActiveWindow.WindowState = xlNormal ActiveCell.Offset(9, 12).Range("Al:A5").Select ActiveWindow.ScrollColumn = 2 ActiveCell.Offset(-8,-18).Range("Al").Select End Sub Figure 3. (continued). ------- SOP #BCO-G-3.1 Revision # 1 July 10, 1997 Page 25 of 25 Table 1. Environmental matrices and their corresponding Excel template file and Access data entry form names. Environmental Matrix Excel Template Filename Access Data Entry Form Name Dermal Wipe Metals DWM.xlt DWM Results Dermal Wipe Pesticides DWP.xlt DWP Results Floor Dust Metals FDM.xlt FDM Results Floor Dust Pesticides FDP.xlt FDP Results Floor Dust PAH FDH.xlt FDH Results Fixed Filter Metals FFM.xlt FFM Results Foundation Soil Metals FSM.xlt FSM Results Organic Vapor Monitor OVM.xlt OVM Results Active airborne PAH Monitor PAH.xlt PAH Results Sill Wipe Metals SWM.xlt SWM Results Volatile Organic Compounds V2C.xlt V2C Results Volatile Organic Compounds V3C.xlt V3C Results Yard Soil Metals YSM.xlt YSM Results Yard Soil PAH YSH.xlt YSH Results Pesticide Combo Air PCA.xlt PCA Results PAH Combo Air PAP.xlt PAP Results ------- |