vvEPA REVISED DRAFT - DO NOT CITE OR QUOTE United States Office of Chemical Safety and Environmental Protection Agency Pollution Prevention Revised Draft Risk Evaluation for C.I. Pigment Violet 29 (Anthra[2,l,9-def:6,5,10-d'eT]diisoquinoline- 1,3,8,10(2H,9H)-tetrone) Systematic Review Supplemental File: Data Quality Evaluation of Environmental Fate and Transport Studies CASRN: 81-33-4 October 2020 ------- REVISED DRAFT - DO NOT CITE OR QUOTE This document is a compilation of tables for the data extraction and evaluation for C.I. Pigment Violet 29 (CASRN 81-33-4). Each table shows the data point or set or information element that was extracted and evaluated from a data source in accordance with Appendix D of the Application of Systematic Review in TSCA Risk Evaluations U.S. EPA (20.1.8). If the source contains more than one data set or information element, the review provides an overall confidence score for each data set or information element that is found in the source. Therefore, it is possible that a source may have more than one overall quality/confidence score. Table of Contents Table 1. EPI Suite™ Modeling Program, U.S. EPA (2012) Table 2. Activated Sludge Study for C.I. Pigment Violet 29, BASF (1999b) Table 3. Biodegradability Study for C.I. Pigment Violet 29, BASF (1999a). 3 6 9 Page 2 of 12 ------- REVISED DRAFT - DO NOT CITE OR QUOTE Table 1. F.PI Suite™ Modeling Program. ' c' Sludj Reference: I .S. I.PA l.sliin;ili(in Programs Interface SuileIM lor Microsoft u Windows. \ 4.11. 2012. Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test substance identity High The test substance was identified by chemical structure. 1 2 2 2. Test substance purity Not rated The metric is not applicable to this study type (SAR). NR NR NR Test Design 3. Study Controls Not rated The metric is not applicable to this study type (SAR). NR NR NR 4. Test Substance stability Not rated The metric is not applicable to this study type (SAR). NR NR NR Test Conditions 5. Test Method Suitability Not rated The metric is not applicable to this study type (SAR). NR NR NR 6. Testing Conditions Not rated The metric is not applicable to this study type (SAR). NR NR NR 7. Testing Consistency Not rated The metric is not applicable to this study type (SAR). NR NR NR 8. System Type and Design Not rated The metric is not applicable to this study type (SAR). NR NR NR Test Organisms 9. Test Organism Degradation Not rated The metric is not applicable to this study type (SAR). NR NR NR 10. Test Organism Partitioning Not rated The metric is not applicable to this study type (SAR). NR NR NR Outcome Assessment 11. Outcome Assessment Methodology Not rated The metric is not applicable to this study type (SAR). NR NR NR 12. Sampling Methods Not rated The metric is not applicable to this study type (SAR). NR NR NR Confounding/ Variable Control 13. Confounding Variables Not rated The metric is not applicable to this study type (SAR). NR NR NR 14. Outcomes Not rated The metric is not NR NR NR Page 3 of 12 ------- REVISED DRAFT - DO NOT CITE OR QUOTE Sludj Reference: I .S. I.PA I'.siiinalion Programs Interlace Sui(eIM lor Microsoft u Windows. \ 4.11. 2012. Unrelated to Exposure applicable to this study type (SAR). Data Presentation and Analysis 15. Data Reporting Not rated The metric is not applicable to this study type (SAR). NR NR NR 16. Statistical Methods and Kinetic Calculations Not rated The metric is not applicable to this study type (SAR). NR NR NR Other 17. Verification or Plausibility of Results Not rated The metric is not applicable to this study type (SAR). NR NR NR 18. QSAR Models High The models in EPI Suite™ have defined endpoints. Chemical domain and performance statistics for each model are known, and unambiguous algorithms are available in the EPI Suite™ documentation and/or cited references to establish their scientific validity. Many EPI Suite™ models have correlation coefficients >0.7, cross-validated correlation coefficients >0.5, and standard error values <0.3; however, correlation coefficients (r2, q2) for the regressions of some environmental fate models {i.e., BIO WIN) are lower, as expected, compared to regressions which have specific experimental values such as water solubility or log Kow (octanol-water partition coefficient). 1 1 1 Sum of scores: 2 3 3 High Medium Low Overall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 1 Overall Score (Rounded): 1 Page 4 of 12 ------- REVISED DRAFT - DO NOT CITE OR QUOTE Sliulj Reference: 1 .S. I.PA l.slimnlion Programs Interlace Sui(eIM lor Microsoft i< Windows. \ 4.11. 2012. >1 and <1.7 >1.7 and <2.3 >2.3 and <3 Overall Quality Level: High Note: The reviewer agreed with this study's overall quality level. Page 5 of 12 ------- REVISED DRAFT - DO NOT CITE OR QUOTE Table 2. Activated Sludge Study for C.T. Pigment Violet 29. ™ ^ """"" J Sludj Reference: I5ASI-". 1«)«)'). Delei'ininalion oil lie Inhihit ion ol' ()\\gen ( onsiiniplion In Acli\aled Sludge In Penlimid I- in llic Acli\aled Sludge Respiration Inhibition lesl according lo CI.I\ I.N 45001 and l('()'J002. Sludj conducted l)\ I5AS1- Akiiengesellschari l.colog\ and l'.n\ ironmenlal Analytics Laboratory of Hcologj IMi^O^h l.udwigshafcn (Siud\ Completion Dale: March 1 iiiro id: 4^3i542 Note: Conducted according to OECD Guideline 209 Domain Metric Qualitative Determination [Le., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test substance identity High CAS, chemical name, production number and date, as well as the specific form of the test substance are all reported. 1 2 2 2. Test substance purity High Purity 98.9% reported by analysis 1 1 1 Test Design 3. Study Controls High Blank Control was included and deviation of blank control was reported as <15%. A reference substance was also included. 1 2 2 4. Test Substance stability High Homogeneity, storage conditions, instability control were all reported and appeared to be appropriate for the test substance. 1 1 1 Test Conditions 5. Test Method Suitability Medium Media concentrations were provided in terms of nominal concentrations (1000 mg/L) and were not measured. The reported limit of solubility (2800 mg/L) was not consistent with the limit of solubility of this chemical. 2 1 2 6. Testing Conditions High Testing conditions were recorded and were suitable for the test substance 1 2 2 7. Testing Consistency High Three blank controls, the test substance were conducted under the same conditions. 1 1 1 Page 6 of 12 ------- REVISED DRAFT - DO NOT CITE OR QUOTE Sludj Reference: I5ASI-". I1)1)1). Delei'ininalion of (lie Inhibition of 0\>gcn Consumption In Acli\alcd Sludge In Pcnlimid 1- in llie Acli\alcd Sludge Respiration Inhihilion lesl according lo (.I P. I.N 45001 and l('() 9002. Sludj conducled In I5AS1- Aklicngcscllschafl Hcolog\ and l'.n\ ironmenlal .\nal> 1 ics l.ahoralon of l'lcolog\ l.udwigshafcn (S(ud\ Completion Dale: March I')1)')). lll-iKO II): 4"73I542 8. System Type and Design Not Rated This metric is not applicable as this is not an equilibrium test NR NR NR Test Organisms 9. Test Organism Degradation High Inoculum source was a laboratory wastewater plant treating municipal and synthetic sewage; the dry substance concentration of the inoculum was reported as 1 g/L 1 2 2 10. Test Organism Partitioning Not Rated This metric is not applicable as this is not a partitioning study NR NR NR 11. Outcome Assessment Methodology High The outcome assessment methodology is acceptable to determine the inhibition of oxygen consumption by activated sludge. 1 1 1 Outcome Assessment 12. Sampling Methods Medium Sampling methods were not specifically discussed, but the results of daily analysis of the test variables were reported so this is not expected to impact the results of the test. 2 1 2 Confounding/ Variable Control 13. Confounding Variables High One study group was used as this was conducted as a limit test. No confounding variables were observed or reported by the study authors. 1 1 1 14. Outcomes Unrelated to Exposure High Deviation of blank controls were reported to be <15%, which demonstrated the health of the test organism. 1 1 1 Data Presentation and Analysis 15. Data Reporting High Study was conducted as a limit test, so no effects were observed in the study group. Study authors calculated and reported EC20, EC50, and EC80 of reference substance as well as the control. 1 2 2 Page 7 of 12 ------- REVISED DRAFT - DO NOT CITE OR QUOTE Sludj Reference: I5ASI-". I1)1)1). Dclerminalion ol'llie Inhibition nl'Owiien ( onsii in pi ion In Acli\aled Sludge In Penlimid 1- in (lie Acli\aled Sludge Respiration Inhibition lost according lo (.I P. I.N 45001 iind l('() 9002. Sludj conducted In I5AS1- Aklicngcscllschari Hcnlngj and l'.n\ immncnlal An;il\lies l.ahoralon of l-'.colog\ IMi^O^ft l.udw igshalcn (S(ud\ ( <1111 plot ion Dale: March 1')«)«) i. lll-iKO II): 4"73I542 16. Statistical Methods and Kinetic Calculations Not Rated Statistical analysis was not conducted as no adverse effects were reported. NR NR NR 17. Verification or Plausibility of Results High Reported values were within expected range as defined by the reference substance, 3,5- dichlorophenol. 1 1 1 Other 18. QSAR Models Not Rated QSAR models were not used as part of this study. *Note that this metric has been updated, as it was originally evaluated for a metric that is not part of the data quality criteria for fate data. NR NR NR Sum of scores: 16 19 21 High Medium Low Overall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 1.105 Overall Score (Rounded): 1.1 >1 and <1.7 >1.7 and <2.3 >2.3 and <3 Overall Quality Level: High Page 8 of 12 ------- REVISED DRAFT - DO NOT CITE OR QUOTE Table 3. Tiiodegrndnhilitv Study for C.T. Pigment Violet 29. ™ ^ ) Sludj Reference: I5ASI-". I«W«). Delei'ininalion oil lie liiodegradahililt of Penlimid I- in llie Mannmelric Kespirnmeln lost according lo CI.P. I.N 45001 and IS()*)002. Sludj conducted In I5AS1- Aklicngcsellschafl l-'.colog\ and l-'.n\ ironnienlal \nal> 1 ics Laboratory of l-'.colog\ D-G^OSh 1 .imIwi^shaleii (Mud> Completion Dale: .Inl> W)). HIIRO II): 4"\5I543 Note: Conducted according to OECD Guideline 30IF Domain Metric Qualitative Determination [Le., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test substance identity High CAS, chemical name, production number, state and date of production were all reported 1 2 2 2. Test substance purity High Purity reported as 98.9%. 1 1 1 Test Design 3. Study Controls High Blank control and reference substance were included (Aniline); deviation and control chemical were acceptable according to test validity criteria of the guideline. 1 2 2 4. Test Substance stability High Homogeneity, storage conditions, instability controls were all reported. 1 1 1 5. Test Method Suitability Medium Media concentrations were provided in terms of nominal concentrations 1 (100 mg/L) which was far higher than the limit of solubility. A A Test Conditions 6. Testing Conditions High Biodegradation values were measured and reported (28 days) according to reporting recommendations of Guideline. Test temperatures throughout the test were not explicitly reported, but the study authors indicated that the test was conduicted "at room temperature". l 2 2 7. Testing Consistency High Five control samples and seven test samples were conducted under the same condition. l 1 1 Page 9 of 12 ------- REVISED DRAFT - DO NOT CITE OR QUOTE Sludj Reference: I5ASI-". I«)«)«). Dclcrminalion oil lie liiodegradal>ilil> olPcnlimid I- in llie Mannmelric Respironiellest according lo CI.P. I.N 45001 and ISO')002. Sludj conducted In I5AS1- AkliciiiiCNcllschiil'l l-lcologj and Hn\ ironmenlal \nal> 1 ics Laboratory of l-'.colog\ I)-(i705(i l.udwigsharen (Sludj ( omplclion Dale: Jul* I')')')). III.KO II): 4"\5I543 8. System Type and Design Not Rated Not an equilibrium test. NR NR NR Test Organisms 9. Test Organism Degradation High Inoculum source reported as municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage which is appropriate. 1 2 2 10. Test Organism Partitioning Not Rated This is not a partitioning test. NR NR NR Outcome Assessment 11. Outcome Assessment Methodology High The test methodology addressed the intended outcome of interest (biodegradation according to the parameters measured). 1 1 1 12. Sampling Methods Medium Sampling methods were not specifically discussed, but the results of daily analysis of the test variables were reported. 2 1 2 13. Confounding Variables Medium The result (Measured BOD) of one blank sample deviated from other 6 test samples. The authors acknowledged and disregarded this sample. 2 1 2 Confounding/ Variable Control 14. Outcomes Unrelated to Exposure Medium 6 out of 7 test samples showed similar results, the degradation rate of all test samples did not show any inhibition from the test substance; One blank sample showed anomalous results discussed by the authors. The viability of organism was well maintained. 2 1 2 Data Presentation and Analysis 15. Data Reporting High The study received a high rating for this metric 1 2 2 16. Statistical Methods and Kinetic Calculations Medium No statistical analyses were conducted; however, sufficient data were provided to conduct 2 1 2 Page 10 of 12 ------- REVISED DRAFT - DO NOT CITE OR QUOTE Sliulj Reference: I5ASI-". I«)«)«). Dclerminalion oil lie liiodegradal>ilil> of Pen limid I- in (lie Mannmelric Respironiellest according lo CLP. I.N 45001 and IS()')002. Sliulj conducted In I5AS1- Aklicngcscllschafl l-lcologj and l-'n\ ir«uiiiieiil;il \n;il> 1 ics Laboratory of Lcolog\ I)-(i705(i Ludwigshafen (Sliulj ( omplclion l);i(e: .1 ul> l«),)«)). III.KO II): 4"\5I543 an independent blaliblieal analysis. Other 17. Verification or Plausibility of Results High Reported values were within expected range as defined by reference substance(s); Aniline. 1 1 1 18. QSAR Models Not Rated QSAR models were not used as part of this study. NR NR NR Sum of scores: 20 20 25 High Medium Low Overall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 1.250 Overall Score (Rounded): 1.3 >1 and <1.7 >1.7 and <2.3 >2.3 and <3 Overall Quality Level: High Page 11 of 12 ------- References REVISED DRAFT - DO NOT CITE OR QUOTE BASF. (1999a). Determination of the biodegradability of perylimid F in the manometric respirometry test according to GLP, EN 45001 and ISO 9002. Ludwigshafen: BASF Aktiengesellschaft Ecology and Environmental Analytics Laboratory of Ecology D-67056. BASF. (1999b). Determination of the inhibition of oxygen consumption by activated sludge by perylimid F in the activated sludge respiration inhibition test according to GLP, EN 45001 and ICO 9002. Ludwigshafen: BASF Aktiengesellschaft Ecology and Environmental Analytics Laboratory of Ecology D-67056. U.S. EPA. (2012). Estimation Programs Interface Suite™ for Microsoft® Windows, v 4.1 1 [Computer Program], Washington, DC. Retrieved from https://www.epa.gov/tsca-screening-tools/epi- suitetm-estimation-program-interface U.S. EPA. (2018). Application of systematic review in TSCA. risk evaluations. (740-P1-8001). Washington, DC: U.S. Environmental Protection Agency, Office of Chemical Safety and Pollution Prevention, https://www.epa.gov/sites/production/files/2018- 06/documetrts/final application of sr in ts< df. Page 12 of 12 ------- |