£%	United States
Environmental Protectio
\r ^1	Agency
Office of Water
EPA 823-D-21-004
October 2021
Frequently Asked Questions: Implementing EPA's 2016
Selenium Criterion in Clean Water Act Sections 303(d) and
305(b) Assessment, Listing, and Total Maximum Daily Load
Programs
Draft

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Frequently Asked Questions: Implementing EPA's
2016 Selenium Criterion in Clean Water Act
Sections 303(d) and 305(b) Assessment, Listing, and
Total Maximum Daily Load Programs
Draft
While this document cites statutes and regulations that contain requirements applicable to water
quality standards, it does not impose legally binding requirements on EPA, states, authorized
tribes, other regulatory authorities, or the regulated community, and may not apply to a
particular situation based upon the circumstances. EPA, state, tribal and other decision makers
retain the discretion to adopt approaches on a case-by-case basis that differ from those provided
in this technical support document as appropriate and consistent with statutory and regulatory
requirements. EPA may update this document as new information becomes available. In addition
to this document, EPA has related documents that provide considerations and recommendations
on implementing the national CWA section 304(a) recommended selenium criterion for
freshwater, which are available at EPA's selenium website: https:/fwww. eya. sov wac aauatic-
life-criterion-seleniiim.
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Table of Contents
Introduction	
Ql: How should states and authorized tribes implement the national CWA section 304(a)
recommended selenium criterion in their assessment and CWA section 303(d) listing programs?
	11
Q2: What data should states and authorized tribes assemble and evaluate to complete water
quality assessments with the national CWA section 304(a) recommended selenium criterion?... 13
Q3: How should states and authorized tribes describe their assessment methodology for
evaluating selenium data?	13
Q4: Should states and authorized tribes include the collection of fish tissue and water column
data in their monitoring programs to assess for selenium?	14
Q5: How should states and authorized tribes complete assessments and CWA section 303(d)
listings for waterbodies that only have selenium water column data?	15
Q6: How should states and authorized tribes complete selenium assessments and CWA section
303(d) listings forfishless waters?	15
Q7: How should states and authorized tribes complete selenium assessments and CWA section
303(d) listings when there are data for multiple fish species?	15
Q8: How should states and authorized tribes complete selenium assessments and CWA section
303(d) listings when there are multiple samples for a single fish species?	16
Q9: How should the selenium fish tissue frequency of "not to be exceeded" be applied in the
assessment and CWA section 303(d) listing programs?	16
Q10: How should states and authorized tribes complete assessments and CWA section 303(d)
listings when steady-state conditions are not present?	17
Ql 1: How should states and authorized tribes implement the selenium water column intermittent
element in assessments and CWA section 303(d) listings?	17
Q12: How can states and authorized tribes remove selenium impairments from their CWA
section 303(d) lists?	18
Q13: Can states and authorized tribes develop a water column criterion element for selenium that
considers site-specific conditions for assessment, 303(d) listing, and TMDL development?	18
Q14: Which criterion elements of the recommended selenium criterion should be used to develop
TMDLs?	19
Q15: How should TMDLs account for the intermittent criterion element of the national CWA
section 304(a) recommended selenium criterion?	20
Q16: Does EPA have recommendations that address whether a receiving waterbody should be
considered lentic or lotic for assessments and 303(d) listings?	20
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Tables
Table 1. Summary of the Recommended Freshwater Selenium Ambient Chronic Water Quality Criterion
for Protection of Aquatic Life	9
Table 2. Matrix of Assessment Scenarios for the National CWA Section 304(a) Recommended Selenium
Criterion1	12
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List of Acronyms
jig/L
micrograms per liter
CFR
Code of Federal Regulations
CWA
Clean Water Act
EPA
Environmental Protection Agency
FAQ
Frequently asked question
mg/kg
milligrams per kilogram
NPDES
National Pollutant Discharge Elimination System
TMDL
Total Maximum Daily Load
USEPA
United States Environmental Protection Agency
WQBEL
Water Quality-Based Effluent Limit
WQC
Water Quality Criterion
WQS
Water Quality Standard

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Definitions
Bioaccumulation
The uptake and retention of a chemical by an aquatic organism from all surrounding media (e.g.,
water, food, sediment).1
Dissolved total selenium
All species of selenium that are dissolved into the water column and that would be measured in a
water sample after it has been passed through a 0.45 |im membrane filter. 2
Performance-based approach
A water quality criterion that is a transparent process, such as a criterion derivation methodology,
rather than a specific outcome, such as a concentration of a pollutant. This process or
methodology is sufficiently detailed and has suitable safeguards that ensure predictable,
repeatable outcomes. Once approved by EPA, this approval would also serve for CWA purposes
as the approval of each outcome generated from following that process or method.3
Steady-state
An organism is in steady-state when the rates of chemical uptake and depuration are equal and
tissue concentrations remain constant over time.4 For the purposes of the national CWA section
304(a) recommended selenium criterion, steady-state refers to conditions where sufficient time
has passed after the introduction of a new or increased discharge of selenium into a water body
so that fish tissue concentrations of selenium are no longer increasing.5
Water quality criterion element
A magnitude, frequency, and duration for a particular media type. The water quality criterion
elements for selenium are related through a hierarchy, with fish tissue criterion elements having
primacy over water column criterion elements, and the egg-ovary criterion element having
primacy over all other criterion elements.
1	USEPA. 2003. Methodology for Deriving Ambient Water Quality Criteria for the Protection of Human Health
(2000) Volume 2: Development of National Bioaccumulation Factors. EPA-882-R-03-030. U.S. Environmental
Protection Agency, Office of Water, Washington, DC. pp. 1-4.
2	Protho, M.G. USEPA. 1993. Memorandum: Office of Water Policy and Technical Guidance on Interpretation and
Implementation of Aquatic Life Metals Criteria. Office of Water, Washington DC.
https://www.epa.gov/sites/production/files/2019-03/documents/metals-criteria-interpret-aalife-memo.pdf
3	EPA Review and Approval of State and Tribal Water Quality Standards 65 Fed. Reg. 24641 (Apr. 27, 2000)
4	USEPA. 2003. Methodology for Deriving Ambient Water Quality Criteria for the Protection of Human Health
(2000) Volume 2: Development of National Bioaccumulation Factors. EPA-882-R-03-030. U.S. Environmental
Protection Agency, Office of Water, Washington, DC. pp. 1-4.
https://nepis.epa.gov/Exe/ZvPDF.cgi/P1005EZQ.PDF?Dockev=P 1005EZQ.PDF
5	USEPA. 2021. 2021 Revision to: Aquatic Life Ambient Water Quality Criterion for Selenium-Freshwater 2016.
EPA 822-R-21-006. U.S. Enviromnental Protection Agency, Office of Water, Washington DC.
https://www.epa.gov/svstem/files/documents/2021-08/selenium-freshwater2016-2021-revision.pdf
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Introduction
EPA '.s National CWA section 304(a) Recommended Chronic Aquatic Life Selenium Criterion
in Freshwater
In 2016, the United States Environmental Protection Agency (EPA) updated its national Clean
Water Act (CWA) section 304(a) recommended chronic aquatic life criterion for selenium in
freshwater systems to reflect the latest scientific information. This information indicates that
toxicity to aquatic life is driven by dietary exposures and that the reproductive life-stages of egg-
laying vertebrates are the most sensitive to the toxic effects of selenium. The criterion has four
criterion elements: (1) a fish egg-ovary criterion element; (2) a fish whole-body and/or muscle
criterion element; (3) a water column criterion element (one value for lentic and one value for
lotic aquatic systems); and (4) a water column intermittent criterion element (to account for
potential chronic effects from short-term exposures to high concentrations in lentic and lotic
aquatic systems) (see Table 1). Under EPA's 2016 CWA section 304(a) recommended selenium
criterion the fish tissue criterion elements have primacy over water column elements, except
where there are no fish, where fish tissue data do not meet state or tribal quality assurance
procedures, or for water bodies with new discharges where selenium concentrations in fish tissue
might not have stabilized. EPA also recommends that the egg-ovary tissue criterion element has
primacy over whole-body and muscle tissue criterion elements.
Toxicity data indicate that the selenium concentration in fish eggs and ovaries is the most robust
and consistent measurement endpoint directly tied to adverse reproductive effects in aquatic
organisms. Toxicity to developing embryos and larvae is directly linked to egg selenium
concentration.6 EPA derived the whole-body, muscle tissue, and water column elements from the
egg-ovary element so that states and authorized tribes could more readily implement water
quality criteria (WQC) based on EPA's national CWA section 304(a) recommended selenium
criterion. The assessment of the available data on chronic selenium exposure for fish,
invertebrates, and amphibians indicates that a criterion element derived from fish is expected to
be protective of the aquatic community, since other taxa appear to be less sensitive to selenium
than fish. EPA did not develop an acute criterion for selenium when it updated the chronic
criterion because although selenium may cause acute toxicity at high concentrations, the most
deleterious effects on aquatic organisms are due to selenium's bioaccumulative properties. The
chronic effects of bioaccumulated selenium occur at lower concentrations than acute effects.
In the case of bioaccumulative compounds like selenium, acute toxicity studies do not address
risks that result from chronic exposure to chemicals via the diet (through the food web pathway).
Such studies also do not account for the accumulation kinetics of many bioaccumulative
compounds, such as selenium, and may underestimate effects from long-term accumulation in
some types of aquatic systems. As described in EPA's 2021 Revision to: Aquatic Life Ambient
Water Quality Criterion for Selenium-Freshwater 2016 (hereafter referred to as Aquatic Life
6 USEPA (U.S. Environmental Protection Agency). 2021. 2021 Revision to: Aquatic Life Ambient Water Quality
Criterion for Selenium-Freshwater 2016. EPA 822-R-21-006. U.S. Environmental Protection Agency, Office of
Water, Washington, DC. https://www.epa.gov/svstem/files/documents/2021-08/selenium-freshwater2016-2Q21-
revision, pdf
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Ambient Water Quality Criterion for Selenium-Freshwater 2016), EPA also included an
intermittent exposure criterion element to provide protection from the most significant effects of
selenium toxicity, reproductive toxicity, by protecting against selenium bioaccumulation in the
aquatic ecosystem resulting from short-term, high concentration exposure events.7 EPA
recommends, as stated in the Aquatic Life Ambient Water Quality Criterion for Selenium-
Freshwater 2016, that states and authorized tribes8 adopt into their water quality standards
(WQS) a selenium criterion that includes all four criterion elements.9 For more information see
EPA's Aquatic Life Ambient Water Quality Criterion for Selenium-Freshwater 2016, which can
be found at https://www.epa.gov/svstem/files/documents/2021-08/selenium-freshwater2016-
2021-revision.pdf
7	Ibid.
8	Throughout this document and in the CWA. the term "states" means the fifty states, the District of Columbia, the
Commonwealth of Puerto Rico, the United States Virgin Islands, Guam, American Samoa, and the Commonwealth
of the Northern Mariana Islands. The term "authorized tribe" means those federally recognized Indian tribes with
authority to administer a CWA WQS program.
9	USEPA.2021. 2021 Revision to: Aquatic Life Ambient Water Quality Criterion for Selenium-Freshwater 2016.
EPA 822-R-21-006. U.S. Enviromnental Protection Agency, Office of Water, Washington DC.
https://www.epa.gov/svstem/files/documents/2021-08/selenium-freshwater2016-2021-revision.pdf
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Table 1. Summary of the Recommended Freshwater Selenium Ambient Chronic Water Quality
Criterion for Protection of Aquatic Life.
Media Type
Fish Tissue1
Water Column4
Criterion
Element
Egg-ovary 2
Fish Whole-body
or Muscle3
Monthly
Average
Exposure
Intermittent Exposure5
Magnitude
15.1 mg/kg dry
weight
8.5 mg/kg dry
weight whole-
body
or
11.3 mg/kg dry
weight muscle
(skinless, boneless
fillet)
1.5 (ig/L in lentic
aquatic systems
3.1 (ig/L in lotic
aquatic systems
WQClnt =
WQC^o-fiay Cbkgrndi.^- f int.
f int
Duration
Instantaneous
measurement6
Instantaneous
measurement6
30 days
Number of days/month with an
elevated concentration
Frequency
Not to be
exceeded
Not to be exceeded
Not more than
once in three
years on average
Not more than once in three
years on average
1.	Fish tissue elements are expressed as steady-state.
2.	Egg/ovary supersedes any whole-body, muscle, or water column element when fish egg-ovary concentrations are measured,
except as noted in footnote 4 below.
3.	Fish whole-body or muscle tissue supersedes water column element when both fish tissue and water concentrations are
measured, except as noted in footnote 4 below.
4.	Water column values are based on dissolved total selenium in water and are derived from fish tissue values via
bioaccumulation modeling. When selenium inputs are increasing, water column values are the applicable criterion element in
the absence of steady-state condition fish tissue data.
5.	Where WQC3o-day is the water column monthly element for either lentic or lotic waters; Cbkgmd is the average background
selenium concentration; and tint is the fraction of any 30-day period during which elevated selenium concentrations occur,
with fmt assigned a value >0.033 (corresponding to 1 day).
6.	Fish tissue data provide instantaneous point measurements that reflect integrative accumulation of selenium over time and
space in fish population(s) at a given site.
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Selenium Technical Support Materials
EPA has prepared a four-volume set of documents to provide recommendations to states,
authorized tribes, and other agencies for implementing WQC based on the national CWA section
304(a) recommended selenium criterion.10 These four documents constitute the Technical
Support Materials for EPA's Aquatic Life Ambient Water Quality Criterion for Selenium-
Freshwater 2016.11 Each document of the set focuses on a specific aspect of implementation of
the national CWA section 304(a) recommended selenium criterion. Together, these four EPA
documents provide information that will assist states and authorized tribes with adopting WQC
based on EPA's CWA section 304(a) recommended selenium criterion and implementing it in
various CWA programs.
1)	Technical Support for Adopting and Implementing EPA's Selenium 2016 Criterion in
Water Quality Standards, Draft, provides recommendations for the adoption and
implementation of the national CWA section 304(a) recommended selenium criterion,
including the various flexibilities available to states and tribes using WQS tools.
2)	Technical Support for Fish Tissue Monitoring for Implementation of EPA's 2016 Selenium
Criterion, Draft: provides an overview on how to establish or enhance existing fish tissue
monitoring programs to facilitate implementation of the fish tissue-based criterion
elements in the national CWA section 304(a) recommended selenium criterion.
3)	Frequently Asked Questions: Implementing Water Quality Standards Based on EPA's
2016 Recommended Selenium Criterion in Clean Water Act Section 402 NPDES Permits,
Draft: is intended to help National Pollutant Discharge Elimination System (NPDES)
permit writers understand what permitting guidance (i.e., state or tribal implementation
procedures) may be appropriate to implement state and authorized tribal WQS based on
EPA's CWA section 304(a) recommended selenium criterion. This set of FAQs also
provides recommendations on how to establish water quality-based effluent limits
(WQBELs) in NPDES permits.
4)	Frequently Asked Questions (FAQs): Implementing the 2016 Selenium Criterion in Clean
Water Act Sections 303(d) and 305(b) Assessment, Listing, and Total Maximum Daily
Load (TMDL) Programs, Draft, provides information on how to complete assessments,
list impaired waters, and develop TMDLs based on EPA approved WQS that adhere to
EPA's national CWA section 304(a) recommended selenium criterion, including all four
elements.
111USEPA. 2021. 2021 Revision to: Aquatic Life Ambient Water Quality Criterion for Selenium-Freshwater 2016.
EPA 822-R-21-006. U.S. Environmental Protection Agency, Office of Water, Washington DC.
https://www.epa.gov/sYStem/files/documents/2021-08/selenium-freshwater2016-2021-revision.pdf
11 Ibid.
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Ql: How should states and authorized tribes implement the national
CWA section 304(a) recommended selenium criterion in their
assessment and CWA section 303(d) listing programs?
Al: The national CWA section 304(a) recommended selenium criterion is structured to enable
assessment of waterbodies using existing and readily available water quality-related data and
information (collectively referred to as data for the remainder of this document) for any element
of the criterion. Table 1 provides an assessment matrix indicating whether the available data
supports an impairment determination for nine aquatic life use assessment scenarios using the
recommended criterion. Each assessment scenario is further explained following the table. Note
that the nine scenarios assume that the waterbody is in steady-state with any selenium sources.
Information on evaluating existing and readily available data for assessment purposes is
discussed in Q2. Information on performing assessments for waterbodies not in steady-state12-13
with selenium sources is provided in Question 10.
Assessment Scenario Descriptions
Assessment scenarios 1 through 6 cover water quality situations for which data are available to
assess the fish tissue component and data are either available, not available, or insufficient to
assess the water column component. For purposes of this document the word "component" is
used to refer to the categories of data that can be used to make an assessment - fish tissue and
water column. Each of these categories has corresponding elements of the criterion associated
with them, as described in Table 1.
Scenarios 1. 2, and 3: Available data indicate that the waterbody does not exceed the fish
tissue component. The recommended criterion is structured such that the fish tissue
criterion elements supersede the water column criterion elements. Hence, if the results for
the fish tissue and water column components do not agree or if water column data are
insufficient or not available, the assessment decision should be based on results for the
fish tissue component. The national CWA section 304(a) recommended selenium
criterion is met in all three scenarios.
12	For the purposes of the national CWA section 304(a) recommended selenium criterion, steady-state refers to
conditions where sufficient time has passed after the introduction of a new or increased discharge of selenium into a
water body so that fish tissue concentrations of selenium are no longer increasing. An organism is in steady-state
when the rates of chemical uptake and depuration are equal and tissue concentrations remain constant over time. The
definition does not apply to the discussion of steady-state modeling or conditions in the "Technical Support
Document for Water Quality-based Toxics Control" (EPA/505/2-90-001, March 1991).
www.epa.gov/npdes/pubs/owm0264.pdf.
13	EP A's Aquatic Life Ambient Water Quality Criterion for Selenium-Freslmater 2016 indicates that, after new
selenium inputs are added to the waterbody, "EPA estimates that the concentration of selenium in fish tissue will not
reach steady-state for several months in lotic systems and longer time periods (e.g., 2-3 years) in lentic systems.
Achievement of steady-state in an aquatic system also depends on the hydrodynamics of the aquatic system,
(particularly reservoirs with multiple riverine inputs), the location of the selenium input and the particular food web.
EPA expects the time needed to achieve steady-state with new or increased selenium inputs to be site-specific."
www.epa.gov/sites/production/files/2016-07/documents/aquatic life awqc for selenium - freshwater 2016.pdf.
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Table 2. Matrix of Assessment Scenarios for the National CWA Section 304(a)
Recommended Selenium Criterion1
Water Column Component5
Not Exceeded
Exceeded
Insufficient or Not
Available
Exceeded
S n 	
, Not
(Scenario 1)	(Scenario 2)
Criterion Met	Criterion Met
(Scenario 3)
Criterion Met
§ Exceeded
m s
s "
(Scenario 4)	(Scenario 5)
Criterion Not Met Criterion Not Met
(Scenario 6)
Criterion Not Met
o
Available
(Scenario 7)
Criterion Met
(Scenario 8)
Criterion Not Met
(Scenario 9)
Not Assessed
Notes: 1 Decisions assume steady-state conditions.
2	Fish tissue component includes the following two criterion elements: (a) fish egg-ovary and (b) fish whole-
body and/or muscle tissue.
3	There is no primacy between fish whole-body and muscle criterion elements.
4	The fish egg-ovary criterion element supersedes the fish whole-body and/or muscle criterion element when
both types of data are available.
5	Water column component includes the following two criterion elements: (a) monthly average exposure and
(b) intermittent exposure criterion elements. The duration component of both of these elements applies to
any 30-day period.
Scenarios 4. 5. and 6: Available data indicate that the waterbody exceeds the fish tissue
component. The recommended criterion is structured such that the fish tissue criterion
elements supersede the water column criterion elements. Hence, if the results for the fish
tissue and water column components do not agree or if water column data are insufficient
or not available, the assessment decision should be based on the results for the fish tissue
component. The national CWA section 304(a) recommended selenium criterion is not
met in all three scenarios and the waterbody-pollutant combination is impaired.
Note that the national CWA section 304(a) recommended selenium criterion is structured such
that the fish egg-ovary criterion element supersedes the fish whole-body and/or muscle criterion
element. Hence, if the results for the fish egg-ovary criterion element and the fish whole-body
and/or muscle criterion elements do not agree, the assessment for the fish tissue component
should be based on the results for the fish egg-ovary criterion element.
Scenarios 7 through 9 cover water quality assessment situations for which data are not available
to assess the fish tissue component and data are either available, not available, or insufficient to
assess the water column component.
Scenario 7: Available data indicate that the waterbody does not exceed the water column
component and data are insufficient or not available to assess the fish tissue component.
The recommended criterion is structured such that water quality assessments can be made
in the absence of fish tissue data using the water column criterion elements of the
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criterion. The national CWA section 304(a) recommended selenium criterion is met in
this scenario.
Scenario 8: Available data indicate that the water column component is exceeded and
data are insufficient or not available to assess the fish tissue component. The national
CWA section 304(a) recommended selenium criterion is not met in this scenario and the
waterbody-pollutant combination is impaired.
Scenario 9: Data are insufficient or not available to assess the water column and the fish
tissue components. Therefore, the national CWA section 304(a) recommended selenium
criterion cannot be assessed. EPA recommends that states and authorized tribes collect
fish tissue and/or water column data so that an assessment decision can be made.
Q2: What data should states and authorized tribes assemble and evaluate
to complete water quality assessments with the national CWA section
304(a) recommended selenium criterion?
A2: States and authorized tribes are required under Title 40 of the Code of Federal Regulations
(CFR) section 130.7(b)(5) to assemble and evaluate all existing and readily available water
quality-related data and information when conducting water quality assessments to determine
which waters belong on their CWA section 303(d) lists. To complete water quality assessments
with the national CWA section 304(a) recommended selenium criterion, states and authorized
tribes should assemble and evaluate all existing and readily available water column and fish
tissue data and information relevant to their jurisdictions, including such data and information
collected by other stakeholders. The extent to which a state or authorized tribe uses the data and
information to make an assessment determination is based on that evaluation. A state or
authorized tribe must provide a technical, science-based rationale for not using certain data and
information to conduct water quality assessments under section 40 C.F.R 130.7(b)(6)(iii).
Q3: How should states and authorized tribes describe their assessment
methodology for evaluating selenium data?
A3: States and authorized tribes are required under Title 40 of the Code of Federal Regulations
section 130.7(b)(6)(i) to submit to EPA Regional Administrator a "description of the
methodology used to develop the list" as part of their 303(d) list submissions. An assessment
methodology constitutes the decision-making process that a state or authorized tribe uses to
determine the water quality attainment status of waters within their jurisdiction. The
methodology should describe how data are evaluated and used to make water quality attainment
determinations, including data quality, quantity, and representativeness considerations.
Additional information regarding data quality, quantity, and representativeness considerations
are available in EPA's Integrated Reporting memo for the 2006 reporting cycle. In addition, if a
state or authorized tribe decides not to rely on certain available information and data in making
listing decisions, it must provide a technical, science-based rationale consistent with 40 CFR
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130.7(b))(6)(iii)14. EPA encourages states and authorized tribes to make the assessment
methodology available to the public for review and comment prior to, or along with, solicitations
for data. Such engagement helps facilitate stakeholder input to the state's or authorized tribe's
assessment of water quality status, including assessments for selenium.
Q4: Should states and authorized tribes include the collection of fish
tissue and water column data in their monitoring programs to assess for
selenium?
A4: The national CWA section 304(a) recommended selenium criterion provides states and
authorized tribes flexibility in how they design their monitoring plans for selenium. This
flexibility stems from the fact that both the fish tissue and water column criterion elements are
designed to protect against chronic selenium effects. States and authorized tribes should consider
a variety of factors when deciding how to monitor for selenium, including assessment and
implementation needs, representativeness of the results, available resources, and public input.
When possible, EPA recommends that states and authorized tribes collect fish tissue data to
support assessments of the recommended selenium criterion. Fish tissue data reflect integrative
accumulation of selenium over time and space in the fish (see Section 2.7.6 of EPA's Aquatic
Life Ambient Water Quality Criterion for Selenium-Freshwater 2016).15 Measurements of
selenium in fish tissue are most closely linked to the chronic adverse effects of selenium, since
chronic selenium toxicity is based on the food-chain bioaccumulation route, not a direct
waterborne route.
States and authorized tribes that want to collect fish tissue data should take into consideration
species specific factors such as selenium sensitivity, bioaccumulation, mobility (including
anadromous and potamodromous species), spawning period, and fish stocking rates when
designing their monitoring plan. The document Technical Support for Fish Tissue Monitoring for
Implementation of EPA's 2016 Selenium Criterion, Draft16 provides additional information to
support the collection, evaluation, and assessment of fish tissue data for selenium. As discussed
in Section 3.1, many states and authorized tribes have existing fish tissue monitoring programs
that can be leveraged to collect fish tissue data to assess against the fish tissue criterion elements
of the recommended selenium criterion.
14	USEPA. 2005. Guidance for 2006 Assessment, Listing and Reporting Requirements Pursuant to Sections 303(d),
305(b) and 314 of the Clean Water Act. https://www.epa.gov/sites/production/files/2015-10/documents/2006irg-
report.pdf
15	USEPA. 2016. Aquatic Life Ambient Water Quality Criterion for Selenium-Freshwater 2016. EPA 822-R-16-006.
U.S. Enviromnental Protection Agency, Office of Water, Office of Science and Technology, Washington, DC,
https://www.epa.gov/sites/production/files/2016-07/documents/aauatic life awac for selenium -
freshwater 2016.pdf.
16USEPA. 2021. Technical Support for Fish Tissue Monitoring for Implementation of EPA's 2016 Selenium
Criterion. Draft. 823-D-21-002. U.S. Enviromnental Protection Agency, Office of Water, Washington, DC.
https://www.epa.gov/wqc/aquatic-life-criterion-selenium
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Q5: How should states and authorized tribes complete assessments and
CWA section 303(d) listings for waterbodies that only have selenium
water column data?
A5: The national CWA section 304(a) recommended selenium criterion is structured to enable
assessment of waterbodies with data related to any element of the criterion. Hence, states and
authorized tribes that adopt the national CWA section 304(a) recommended selenium criterion
should complete water quality assessments for waterbodies that only have selenium water
column data (and no selenium fish tissue data). Additional information about completing
assessments for these scenarios is available in Q1 (see Scenarios 7 and 8). After a waterbody is
added to a CWA section 303(d) list based on water column data alone, states and authorized
tribes may consider collecting fish tissue data to confirm the assessment determination before
developing a water quality management plan (e.g., TMDL).
Q6: How should states and authorized tribes complete selenium
assessments and CWA section 303(d) listings for fishless water si
A6: The national CWA section 304(a) recommended selenium criterion is structured to enable
assessment of waterbodies with data related to any element of the criterion. Hence, states and
authorized tribes that adopt the national CWA section 304(a) recommended selenium criterion
should complete water quality assessments using the water column criterion elements for fishless
waters.17 Additional information about completing assessments for these scenarios is available in
Q1 (see Scenarios 7 through 9).
Note that the national CWA section 304(a) recommended selenium criterion is designed to
protect all freshwater aquatic life, including populations of fish, amphibians, aquatic
invertebrates, and plants. The criterion elements were derived from fish toxicity values to be
protective of the entire aquatic community, not solely fish species. The criterion elements,
including the water quality criterion elements, are designed to protect aquatic life from the
chronic effects of exposure to selenium in waters both inhabited by fish and not inhabited by
fish.
Q7: How should states and authorized tribes complete selenium
assessments and CWA section 303(d) listings when there are data for
multiple fish species?
A7: The national CWA section 304(a) recommended selenium criterion is designed to protect all
freshwater aquatic life. Hence, if available data and information from one or more fish species
for a waterbody exceeds the criterion, then the criterion is not met and the waterbody-pollutant
combination is impaired. States and authorized tribes should not composite or average data from
17 EPA describes fishless waters as waters with insufficient instream habitat and/or flow to support a population of
any fish species on a continuing basis, or waters that once supported populations of one or more fish species but no
longer support fish (e.g., extirpation) due to temporary or permanent changes in water quality (e.g., selenium
pollution), flow, or instream habitat.
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multiple species. Also, the hierarchical elements of the fish tissue component of the criterion
(i.e., egg-ovary data supersedes muscle/whole-body) apply within each fish species. States and
authorized tribes should not apply the hierarchy across species, as this would be inconsistent
with the procedure used to derive the fish tissue element of the criterion18, which used species-
specific conversion factors to calculate a toxicity value.
Q8: How should states and authorized tribes complete selenium
assessments and CWA section 303(d) listings when there are multiple
samples for a single fish species?
A8: States and authorized tribes have flexibility on how to evaluate individual and composite
samples for a single fish species. The state's or authorized tribe's assessment methodology
should document the decision-making process for this scenario (see Q3). As part of the
methodology, the state or authorized tribe should discuss the process and rationale for combining
and assessing data from individual and/or composite samples of the same species, including how
the time and location of sample collection and size of fish samples were considered. Additional
information on approaches and factors to consider in collecting, evaluating, and assessing data
from individual or composite fish tissue samples is provided in the document Technical Support
for Fish Tissue Monitoring for Implementation of EPA's 2016 Selenium Criterion, Draft19.
Q9: How should the selenium fish tissue frequency of "not to be
exceeded" be applied in the assessment and CWA section 303(d) listing
programs?
A9: The fish tissue criterion elements of the national CWA section 304(a) recommended
selenium criterion each have a frequency of "not to be exceeded." As explained in the criterion
document, this frequency was selected because it may take a prolonged period for fish
communities to recover from exposure to elevated levels of selenium. EPA's current
recommendation of a "once in three years on average" return frequency for aquatic life criteria is
not adequate for selenium (see Section 2.7.7 of EPA's Aquatic Life Ambient Water Quality
Criterion for Selenium-Freshwater 2016 for additional information)20. For assessment and CWA
section 303(d) listing purposes, assessment methodologies of states and authorized tribes should
be consistent with their applicable water quality standards, including the magnitude, duration and
frequency. Hence, for states and authorized tribes that adopt the national CWA section 304(a)
18 USEPA. 2021. 2021 Revision to: Aquatic Life Ambient Water Quality Criterion for Selenium-Freshwater 2016.
EPA 822-R-21-006. U.S. Environmental Protection Agency, Office of Water, Washington, DC.
https://www.epa.gov/svstem/files/documents/2021-08/selenium-freshwater2016-2Q21-revision.pdf
19USEPA. 2021. Technical Support for Fish Tissue Monitoring for Implementation of EPA's 2016 Selenium
Criterion. Draft. 823-D-21-002. U.S. Enviromnental Protection Agency, Office of Water, Washington, DC.
https://www.epa.gov/wac/aauatic-life-criterion-selenium
211 USEPA. 2021. 2021 Revision to: Aquatic Life Ambient Water Quality Criterion for Selenium-Freshwater 2016.
EPA 822-R-21-006. U.S. Enviromnental Protection Agency, Office of Water, Washington, DC.
https://www.epa.gov/svstem/files/documents/2021-08/selenium-freshwater2016-2Q21-revision.pdf
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recommended selenium criterion, their assessment methodologies should be consistent with the
magnitude, duration and frequency component of the recommendation.
Q10: How should states and authorized tribes complete assessments and
CWA section 303(d) listings when steady-state conditions are not
present?
A10: The national CWA section 304(a) recommended selenium criterion is structured to address
circumstances when steady-state conditions are present or not present in the waterbody (see Q1
for the assessment scenario matrix for steady-state conditions). Steady-state conditions may not
be present in the water body when new inputs21 are introduced that will likely result in higher
concentrations of selenium in the food web and a relatively slow increase in the selenium
concentration in fish tissue. According to the national CWA section 304(a) recommended
selenium criterion, the fish tissue criterion elements supersede the water column criterion
elements when the waterbody is in a steady-state condition. When the waterbody is not in steady-
state, the waterbody is not meeting the criterion and is considered impaired if either the fish
tissue or water column criterion elements are exceeded.22
Q11: How should states and authorized tribes implement the selenium
water column intermittent element in assessments and CWA section
303(d) listings?
All: The water column component of the national CWA section 304(a) recommended selenium
criterion includes both an intermittent criterion element and a monthly average water column
criterion element (i.e., 30-day average). The intermittent criterion element is a calculation based
on the known ambient concentration, the monthly average exposure water column criterion
element, and the fraction of any 30-day period during which elevated selenium concentrations
occur. As explained in section 3.3 of EPA's Aquatic Life Ambient Water Quality Criterion for
Selenium-Freshwater 201623, the intermittent criterion element is a re-expression of the chronic
30-day average water column criterion element and will yield the same level of protection as the
monthly average exposure criterion element, provided that the equation uses the average of the
concentrations occurring for (1) the fraction of time defined as being intermittently elevated and
(2) the remaining time defined as being background.
The intermittent criterion element can only be exceeded under the same conditions that would
have caused the monthly average exposure criterion element to be exceeded. Thus, evaluating
data against the intermittent element and the monthly average exposure criterion element will
always result in the same assessment decision. As such, state and authorized tribes may find it
21	EPA describes new inputs as new activities resulting in the release of selenium into a lentic or lotic aquatic
system.
22	USEPA. 2021. 2021 Revision to: Aquatic Life Ambient Water Quality Criterion for Selenium-Freshwater 2016.
EPA 822-R-21-006. U.S. Enviromnental Protection Agency, Office of Water, Washington, DC.
https://www.epa.gov/sYStem/files/documents/2021-08/selenium-freshwater2016-2021-revision.pdf
23	Ibid
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more straightforward to assess the water column component of the recommended criterion using
the monthly average criterion element because it does not require knowledge of the background
conditions or number of days with an intermittent spike.
Q12: How can states and authorized tribes remove selenium
impairments from their CWA section 303(d) lists?
A12: As with any applicable water quality standard, states and authorized tribes that adopt the
national CWA section 304(a) recommended selenium criterion will make assessment decisions
based on an evaluation of existing and readily available water quality data against the criterion
and accompanying assessment methodology. The assessment decision informs whether a
waterbody should be identified as impaired and included on the state's or authorized tribe's
CWA section 303(d) list and assigned a priority ranking for TMDL development. Selenium
impairments included on the CWA section 303(d) list may be excluded from subsequent lists
(sometimes described as "delisting") for several reasons, including: (a) the water quality standard
is met based on the criterion and accompanying assessment methodology, (b) there were flaws in
the original listing, (c) a TMDL was established and approved by the EPA, or (d) other point
source or nonpoint source controls are expected to result in the waterbody meeting water quality
standards.
Q13: Can states and authorized tribes develop a water column criterion
element for selenium that considers site-specific conditions for
assessment, 303(d) listing, and TMDL development?
A13: States and authorized tribes can take one of two approaches for developing a water column
criterion element for selenium that considers site-specific conditions. Under the first approach, if
a state or authorized tribe thinks that the default national water column criterion element is not
appropriate for a waterbody, states and authorized tribes can choose to adopt into their WQS and
submit to EPA for approval a site-specific water column criterion element developed using the
procedures provided in Appendix K of the national CWA section 304(a) recommended selenium
criterion24 or another scientifically defensible method that is protective of the aquatic life use.
Under the second approach, states and authorized tribes can choose to adopt into their WQS and
submit to EPA for approval a set of procedures to translate the fish tissue criterion elements into
a site-specific water column criterion element. This is considered a performance-based approach
to site-specific criteria development.25 Information on both approaches is provided in sections
24	Ibid.
25	In addition to the four-part criterion, states or authorized tribes may want to adopt a method to derive site-specific
water column criterion elements, referred to by EPA in 2000 as a "performance-based approach". EPA first
formalized the concept of a performance-based approach for a WQS in the preamble of the rule EPA Review and
Approval of State and Tribal Water Quality Standards. Here EPA describes this approach as a WQS that is a
transparent process rather than a specific outcome. The state or authorized tribe can adopt a process, such as a
criterion derivation methodology, rather than a specific outcome, such as a concentration of a pollutant. If a state or
authorized tribe adopts a process or methodology that is sufficiently detailed and has suitable safeguards that ensure
predictable, repeatable outcomes, EPA can approve that process as a WQS and this approval would also serve for
CWA purposes as the approval of each outcome generated from following that process or method.
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2.2.1 and 2.2.2 of Technical Support for Adopting and Implementing EPA's 2016 Selenium
Criterion in Water Quality Standards, Draft.26
States and authorized tribes should consider which of the two approaches they want to use when
adopting the national CWA section 304(a) recommended selenium criterion. For example, the
first approach (i.e., EPA approved site-specific criterion) is more straightforward to apply in
assessment, 303(d) listing, and TMDL development and will likely reduce workload on those
programs. Furthermore, the opportunity for public comment on the site-specific water column
criterion element would occur in response to a single CWA section 303(c) action. The second
approach (i.e., performance-based) facilitates an opportunity for states and authorized tribes to
adaptively derive a site-specific water column criterion element to account for the most up-to-
date data for the waterbody using the procedures in the EPA-approved criterion. When a
performance-based approach is used, however, more coordination will likely be needed between
the CWA implementing programs to consistently employ such water column criterion elements,
and the opportunity for public comment on the translated site-specific water column criterion
element would occur in response to different and multiple program actions (e.g., assessment
decisions, or development of TMDLs or permit limits).
Q14: Which criterion elements of the recommended selenium criterion
should be used to develop TMDLs?
A14: States and authorized tribes that adopt the national CWA section 304(a) recommended
selenium criterion should consider developing TMDLs for selenium based on the monthly
average exposure water column criterion element or a site-specific water column criterion
element developed through one of the two approaches described in Q13. As discussed in Ql,
four scenarios (i.e., 4, 5, 6, and 8 in Table 1) exist for which a waterbody can be assessed as
impaired. For three of the scenarios (i.e., 5, 6, and 8 in Table 1), states and authorized tribes
should consider using the monthly average exposure water column criterion element to develop
TMDLs. EPA recommends the approach of using the monthly average exposure water column
criterion element for these scenarios because it was derived to ensure adequate protection of the
fish tissue criterion elements of the criterion. However, for scenario 4 (wherein available data
indicate that the fish tissue criterion element of the criterion is exceeded and the water column
criterion element is not exceeded), states and authorized tribes should consider developing a site-
specific water column criterion element and use it for developing the TMDL. Using the monthly
average exposure water column criterion element under scenario 4 to develop the TMDL could
result in a loading capacity for selenium that exceeds the existing load, which is already resulting
in an exceedance of the fish tissue criterion elements of the criterion in the waterbody. For this
scenario states and authorized tribes should consider developing a site-specific water column
criterion element that would provide adequate protection of the fish tissue criterion elements of
the criterion.
26USEPA. 2021. Technical Support for Adopting and Implementing EPA's 2016 Selenium Criterion in Water
Quality Standards. Draft. EPA 823-D-21-001. U.S. Environmental Protection Agency, Office of Water,
Washington, DC. https://www.epa.gov/wqc/aquatic-life-criterion-selenium
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Q15: How should TMDLs account for the intermittent criterion element
of the national CWA section 304(a) recommended selenium criterion?
A15: For states and authorized tribes that adopt the national CWA section 304(a) recommended
selenium criterion, developing TMDLs based on the monthly average exposure water column
criterion element of the criterion would also address the intermittent water column criterion
element. Section 3.3 of EPA's Aquatic Life Ambient Water Quality Criterion for Selenium-
Freshwater 2016 explains that the intermittent criterion element is a re-expression of the chronic
30-day average water column criterion element of the criterion.27 As such, the monthly average
exposure water column criterion element and the intermittent criterion element of the criterion
are intended to provide the same level of protection of the fish tissue criterion elements of the
criterion.
Permit writers develop water quality-based effluent limitations (WQBELs) to meet the state's or
authorized tribe's EPA-approved water quality standards. Hence, for states and authorized tribes
that adopt the national CWA section 304(a) recommended selenium criterion, the permitting
authorities would use the water column criterion element (either monthly average or intermittent,
as appropriate) and any available TMDL wasteload allocations to establish NPDES permit limits.
Because the intermittent criterion element is a re-expression of the monthly average water
column criterion element, permits that include an intermittent criterion element would be
consistent with wasteload allocations in TMDLs that are based on the monthly average
expression water column criterion element.
Q16: Does EPA have recommendations that address whether a receiving
waterbody should be considered lentic or lotic for assessments and
303(d) listings?
A17: The national CWA section 304(a) recommended selenium criterion includes water column
criterion element values for both lotic and lentic freshwater systems. Generally, classifications of
fresh waters into lotic or lentic categories are made by the state or authorized tribe, based on site-
specific information. States and authorized tribes should ensure that they use transparent,
scientifically defensible methods to classify receiving waterbodies appropriately. Reservoirs with
extended residence times are more likely to demonstrate bioaccumulation dynamics that are
lentic in nature. Section 3.2.4 in EPA's Aquatic Life Ambient Water Quality Criterion for
Selenium—Freshwater 2016 provides further discussion of categories of fresh water. A site-
specific study or use of available hydrology information might be necessary to determine the
residence time of the waterbody so that the application of the lentic or lotic water column
criterion element can be appropriately applied in assessments and CWA section 303(d) listings.
27 USEPA. 2021. 2021 Revision to: Aquatic Life Ambient Water Quality Criterion for Selenium-Freshwater 2016.
EPA 822-R-21-006. U.S. Environmental Protection Agency, Office of Water, Washington, DC.
https://www.epa.gov/svstem/files/documents/2021-08/selenium-freshwater2016-2Q21-revision.pdf
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