US Environmental Protection Agency
Office of Pesticide Programs

Office of Pesticide Programs

Microbiology Laboratory

Environmental Science Center, Ft. Meade, MD

Standard Operating Procedure for

Preparation and Maintenance of the Master Schedule

SOP Number: QA-04-05
Date Revised: 05-01-13


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SOP No. QA-04-05
Date Revised 05-01-13
Page 1 of7

SOP Number

QA-04-05

Title

Preparation and Maintenance of the Master Schedule

Scope

The purpose of this procedure is to provide guidance for the
preparation and maintenance of the Master Schedule for the testing
activities at the Microbiology Laboratory Branch

Application

As a requirement of the GLP standards (42 CFR Part 160), the quality
assurance unit shall maintain a Master Schedule of all studies.



Approval Date

SOP Developer:



Print Name:

Quality Assurance Unit



Print Name:

Branch Chief



Print Name:





Date SOP issued:



Controlled copy number:



Date SOP withdrawn:




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SOP No. QA-04-05
Date Revised 05-01-13
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TABLE OF CONTENTS
Contents	Page Number

1.

DEFINITIONS

3

2.

HEALTH AND SAFETY

3

3.

PERSONNEL QUALIFICATIONS AND TRAINING

3

4.

INSTRUMENT CALIBRATION

3

5.

SAMPLE HANDLING AND STORAGE

3

6.

QUALITY CONTROL

3

7.

INTERFERENCES

3

8. NON-CONFORMING DATA

3

9.

DATA MANAGEMENT

3

10.

CAUTIONS

4

11.

SPECIAL APPARATUS AND MATERIALS

4

12.

PROCEDURE AND ANALYSIS

4

13.

DATA ANALYSIS/CALCULATIONS

7

14.

FORMS AND DATA SHEETS

7

15.

REFERENCES

7


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SOP No. QA-04-05
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1. Definitions

1.	GLP= Good Laboratory Practices

2.	QAU = Quality Assurance Unit

3.	MLB = Microbiology Laboratory Branch located in the Environmental
Science Center, Ft. Meade, Maryland

4.	Abbreviations/definitions are also provided in the text.

2. Health and
Safety

None.

3. Personnel
Qualifications
and Training

Refer to SOP ADM-04, OPP Microbiology Laboratory Training.

4. Instrument
Calibration

Not applicable

5. Sample

Handling and
Storage

Not applicable

6. Quality Control

1.	MLB conforms to 40 CFR Part 160, Good Laboratory Practice Standards.

2.	For quality control purposes, the required information is documented on
the appropriate form (see section 14).

7. Interferences

None

8. Non-
conforming
Data

1.	Data entry errors discovered in the Master Schedule will be immediately
corrected in the electronic version.

2.	If an error is identified in a retired Master Schedule file (i.e., from a
previous year) or a section of the current year's Master Schedule that has
already been archived (section 9.0), the QAU will make the correction
electronically. The QAU will archive the corrected copy of the Master
Schedule.

9. Data

Management

1.	Information pertinent to each study is entered electronically into the
Master Schedule (see section 14) (G:\Data\shared\MLB\Master
Schedule\Current Master Schedule).

2.	The QAU maintains an electronic copy of the Master Schedule file on a
quarterly basis. At the end of each quarter, the QAU sends out the Master
Schedule for the applicable quarter electronically to the Branch Chief and
study directors and archives a pdf copy in the Master Schedule folder on
the G drive. The comprehensive Master Schedule for the fiscal year is
archived annually.

3.	Master Schedule will be archived consistent with SOP ADM-03, Records


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and Archives.

10. Cautions

None

11. Special

Apparatus and
Materials

None

12. Procedure and
Analysis

1.	Organization of the Master Schedule: According to GLPs (see ref. 15.1)
the QAU shall maintain a copy of a Master Schedule of all studies
conducted at the testing facility.

2.	The test substance for each study is identified and described in the study
protocol, statements of work, research proposals, or each (project) study
notebook.

3.	Protocol numbers are unique identifiers for each study conducted in the
laboratory and it is practical to index the Master Schedule by the protocol
number.

12.1 Master
Schedule
Preparation

a. Protocol Number: All product performance test studies are tracked

in the Master Schedule by the protocol number, a unique identifier.

In this column, enter the protocol number or research protocol

number assigned to the study.

i.	For product performance tests conducted as part of the
Agency's Antimicrobial Testing Program (ATP) or research
projects associated with other ongoing programs, e.g.
Homeland Security (HS), Virology (VTP), and Biofilm, a
unique study protocol number is assigned to each study.

ii.	The ATP Study protocol numbers are assigned in numerical
order for each calendar year (e.g., the number assigned to first
protocol of 2013 would be 2013-01; the number assigned to
the second study protocol of 2013 would be 2013-02, etc.).

iii.	Research protocols are assigned numbers, starting with a
prefix R, followed by the year (e.g.2013 ) and chronological
number of the research project, (e.g. the number assigned to
first research protocol in 2013 would be R-2013-01; the
number assigned to second research protocol would be R-
2013-02, etc.).

iv.	The text of the study protocol identifies the title, purpose, test
identification, test substance, controls, sponsor, test facility
proposed experimental start date, proposed experimental
completion date, test system, and experimental design of the


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study.

b.	Test Substance (EPA Reg. No. /Product). A substance or mixture
administered or added to a test system in a study (see ref. 15.1), for
example, EPA-registered hospital disinfectants and tuberculocides.

i. If applicable, enter the EPA Registration Number and product
name (e.g., EPA-registered hospital disinfectant/tuberculocide
to be subjected to efficacy testing). Enter N/A (not
applicable) if there is no EPA Registration Number available.

c.	Study Sponsor/Study Director/Test Coordinator:

i.	Study Sponsor is the individual or entity who initiates and
supports a study [e.g., Antimicrobials Division (AD), OPP
MLB],

ii.	Study Director is the individual responsible for the overall
conduct of the study.

iii.	Test coordinator/Lead analyst is the individual responsible for
developing the data and for compilation/preparation of
(summary) final report.

d.	Test System/Method or Description of Study. Enter the test
system and nature of the study (e.g., test method).

i. Nature of study: Typically, it is listed as test method used in
the study (e.g., AO AC Use Dilution Method or AO AC
Germicidal Spray Products as Disinfectants Test).

e.	Study Initiation Date. Enter the date when data collection activities
in the laboratory are initiated.

f.	QAU Inspection/QAU Inspector. Enter the elements of the
testing/reporting process that have been or will be audited by the
QAU during the duration of the study. Examples of study elements
may include, but are not limited to, laboratory notebooks, the Study
Protocol, certain procedures of the in-progress study, the draft report,
and the final report.

i.	QAU Inspector. Enter the first initial and the last name of the
QAU conducting the audit of each of the pertinent elements
described in section 12.1.

ii.	Date of Audit Report (Completion). Add the date the audit is
conducted and (optional) when the report is sent to the Study
Director and management.

g.	Study Status. Signifies the current status of the study (e.g., Study in


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progress, complete)

i.	Enter the most applicable of the following: Study in progress,
Report in progress, Complete, or Non-conclusive/repeat test.

ii.	Preparation of a final report (see SOP ADM-01, Preparation
of Performance Reports) or data summary is at the discretion
of the Study Director or Branch Chief.

iii.	Enter "Study in progress" to designate the time period
beginning with the study initiation date and ending with the
study completion or study termination date.

iv.	Enter "Report in progress" to designate the time period during
which the report or data summary is being compiled and
reviewed (see SOP ADM-01, Preparation of Performance
Reports).

v.	Enter "Complete" as directed in vi through viii below:

vi.	For studies requiring the development of a report (e.g.,
efficacy testing for the Antimicrobial Testing Program), enter
"Complete" once the final report has been reviewed and
approved by the QAU and Branch Chief.

vii.	For all other studies, enter "Complete" once the Study
Director and/or Branch Chief have reviewed and approved
the summary of the data.

viii.	Enter "Non-conclusive/repeat test" to indicate that the study
was invalidated (e.g., due to an event such as low carrier
counts, etc.) and/or that the study must be repeated. Data
from a study that must be repeated will be compiled and
submitted to the Study Director and QAU for review.

h.	Study Completion Date. Enter the date that the data collection

activities in the laboratory are completed.

i.	Final Report Approval Date (QAU).

i.	For the ATP studies, enter the date, using the six-digit MM-
DD-YY format, that the QAU signed the Quality Assurance
Review Form (see SOP ADM-01; Preparation of Performance
Reports).

ii.	Enter "Not applicable" if the study was invalidated and must
be repeated.

iii.	For research studies associated with a Quality Assurance

	Project Plan, enter the date when the report is approved by the


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Study Director and/or Branch Chief.

12.2 Updating the
Master
schedule

a.	The QAU, Branch Chief, Study Director, or lead analysts may update
the Master Schedule, as needed, to ensure that the schedule provides
an accurate and concise assessment and current status of each study.

b.	The QAU ensures on a regular basis that the data in the Master
Schedule are current and up-to-date.

12.3 Using the
Master
schedule

a. The QAU, in compliance with GLPs (see ref. 15.1), maintains an
electronic (pdf) copy of the Master Schedule of studies (see section
9.0). In addition, the Master Schedule is used to develop test method
auditing schedules (see SOP QA-01, Operations of the Quality
Assurance Unit).

13. Data Analysis/
Calculations

None

14. Forms and Data
Sheets

Test sheets are stored separately from the SOP under the following file name:
Master Schedule QA-04-05_Fl.docx

15. References

1. US EPA Good Laboratory Practices, Title 40 Code of Federal
Regulations(CFR) Part 160


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