U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 SCREENING-LEVEL HAZARD CHARACTERIZATION 3,4-Dichloro-&,&,&-trifluorotoluene (CASRN 328-84-7) The High Production Volume (HPV) Challenge Program1 was conceived as a voluntary initiative aimed at developing and making publicly available screening-level health and environmental effects information on chemicals manufactured in or imported into the United States in quantities greater than one million pounds per year. In the Challenge Program, producers and importers of HPV chemicals voluntarily sponsored chemicals; sponsorship entailed the identification and initial assessment of the adequacy of existing toxicity data/information, conducting new testing if adequate data did not exist, and making both new and existing data and information available to the public. Each complete data submission contains data on 18 internationally agreed to "SIDS" (Screening Information Data Set1'2) endpoints that are screening-level indicators of potential hazards (toxicity) for humans or the environment. The Environmental Protection Agency's Office of Pollution Prevention and Toxics (OPPT) is evaluating the data submitted in the HPV Challenge Program on approximately 1400 sponsored chemicals by developing hazard characterizations (HCs). These HCs consist of an evaluation of the quality and completeness of the data set provided in the Challenge Program submissions. They are not intended to be definitive statements regarding the possibility of unreasonable risk of injury to health or the environment. The evaluation is performed according to established EPA guidance2'3 and is based primarily on hazard data provided by sponsors; however, in preparing the hazard characterization, EPA considered its own comments and public comments on the original submission as well as the sponsor's responses to comments and revisions made to the submission. In order to determine whether any new hazard information was developed since the time of the HPV submission, a search of the following databases was made from one year prior to the date of the HPV Challenge submission to the present: (ChemID to locate available data sources including Medline/PubMed, Toxline, HSDB, IRIS, NTP, AT SDR, IARC, EXTOXNET, EPA SRS, etc.), STN/CAS online databases (Registry file for locators, ChemAbs for toxicology data, RTECS, Merck, etc.), Science Direct and ECHA4. OPPT's focus on these specific sources is based on their being of high quality, highly relevant to hazard characterization, and publicly available. OPPT does not develop HCs for those HPV chemicals which have already been assessed internationally through the HPV program of the Organization for Economic Cooperation and Development (OECD) and for which Screening Initial Data Set (SIDS) Initial Assessment Reports (SIAR) and SIDS Initial Assessment Profiles (SIAP) are available. These documents are presented in an international forum that involves review and endorsement by governmental authorities around the world. OPPT is an active participant in these meetings and accepts these documents as reliable screening-level hazard assessments. 1 U.S. EPA. High Production Volume (HPV) Challenge Program; http://www.epa.gov/chemrtk/index.htm. 2 U.S. EPA. HPV Challenge Program - Information Sources; http://www.epa.gov/chemrtk/pubs/general/guidocs.htm. 3 U.S. EPA. Risk Assessment Guidelines; http://cfpub.epa.gov/ncea/raf/rafguid.cfm. 4 European Chemicals Agency, http://echa.europa.eu. ------- U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 These hazard characterizations are technical documents intended to inform subsequent decisions and actions by OPPT. Accordingly, the documents are not written with the goal of informing the general public. However, they do provide a vehicle for public access to a concise assessment of the raw technical data on HPV chemicals and provide information previously not readily available to the public. 2 ------- U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 Chemical Abstract Service Registry Number 328-84-7 (CASRN) Chemical Abstract Index Name Benzene, l,2-dichloro-4-(trifluoromethyl)- Structural Formula F SMILES: FC(F)(F)c(ccc(c 1 Cl)Cl)c 1 Summary 3,4-Dichloro-d,d,d-trifluorotoluene is a colorless liquid with high vapor pressure and moderate water solubility. It is expected to have moderate mobility in soil. Volatilization of 3,4- dichloro-d,d,d-trifluorotoluene is high based on its Henry's Law constant. The rate of hydrolysis is expected to be negligible. The rate of atmospheric photooxidation is negligible. 3.4-Dichloro-d,d,d-trifluorotoluene is not readily biodegradable. 3,4-Dichloro-d,d,a- trifluorotoluene is expected to have high persistence (P3) and moderate bioaccumulation potential (B2). Acute oral and dermal toxicity of 3,4-dichloro-d,d,d-trifluorotoluene is low in rats and rabbits, respectively. Acute inhalation toxicity in rats is moderate. In a modified oral gavage repeated- dose/ reproductive/developmental toxicity study in rats no systemic effects were observed up to 45 mg/kg-day; the NOAEL for systemic toxicity is 45 mg/kg-day, the highest dose tested. No effects on reproductive or developmental parameters were reported; the reproductive and developmental NOAEL is 45 mg/kg-day, the highest dose tested. 3,4-Dichloro-d,d,a- trifluorotoluene was not mutagenic in bacteria or mammalian cells in vitro. No data are available for chromosomal aberrations. 3,4-Dichloro-d,d,d-trifluorotoluene induced sister chromatid exchange (SCEs) in mouse lymphoma L5178Y cells in the presence of metabolic activation, but weakly induced SCE in the absence of activation. 3,4-Dichloro-d,d,a- trifluorotoluene is irritating to rabbit skin, is not irritating to rabbit eyes and is not a dermal sensitizer in guinea pigs. The 96-h LCso for fish and aquatic invertebrates from 3,4-dichloro-d,d,d-trifluorotoluene is 2.05-2.3 and 1.7 mg/L, respectively. The 96-h ECsoto aquatic plants for growth rate is > 8.6 mg/L. The 21-d LCso for chronic aquatic invertebrates from 3,4-dichloro-d,d,a- trifluorotoluene is 0.16 mg/L. The genetic toxicity (chromosomal aberrations) endpoint was identified as a data gap under the HPV Chemical Challenge Program. 3 ------- U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 The sponsor, Dow AgroSciences LLC, submitted a Test Plan and Robust Summaries to EPA for 3,4-dichloro-d,d,d-trifluorotoluene (CASRN 328-84-7; 9th CI name: benzene, l,2-dichloro-4- (trifluoromethyl)-) on December 18, 2003. EPA posted the submission on the ChemRTK HPV Challenge website on March 5, 2004 (http://www.epa.gov/chemrtk/pubs/summaries/34dichlo/cl5026tc.htm). EPA comments on the original submission were posted to the website on July 26, 2004. Public comments were also received and posted to the website. The sponsor submitted updated/revised documents on August 25, 2005, which were posted to the ChemRTK website on October 6, 2005. 1. Chemical Identity 1.1 Identification and Purity 3,4-dichloro-d,d,d-trifluorotoluene is a colorless liquid with high vapor pressure and moderate water solubility and is an intermediate in the production of pesticides. The purity is reported as 95 to 99.5% when provided in the robust summaries. 1.2 Physical-Chemical Properties The physical-chemical properties of 3,4-dichloro-d,d,d-trifluorotoluene are summarized in Table 1. Table 1. Physical-Chemical Properties of 3,4-dichloro-a,a,d-trifluorotoluene (CI name: Benzene, 1,2 dichloro 4 (trifluoromethyl)1 Property Value CASRN 328-84-7 Molecular Weight 215.0 Physical State Colorless liquid Melting Point -12°C (measured) Boiling Point 173.5°C (measured) Vapor Pressure 1.6 mm Hg at 20°C (measured) Dissociation Constant (pKa) Not applicable Henry's Law Constant 2.9x 10"2 atm-m3/mole (estimated)2 Water Solubility 11.6 mg/L at 23°C (measured) Log Kow 3.843 'The Dow Chemical Company. 2005. Test Plan and Robust Summary for 3,4-Dichloro-alpha, alpha, alpha- trifluorotoluene. Available online at http://www.epa.gov/chemrtk/pubs/summaries/34dichlo/cl5026tc.htm as of June 12, 2012. 2U.S. EPA. 2012. Estimation Programs Interface Suite™ for Microsoft® Windows, v4.10. U.S. Environmental Protection Agency, Washington, DC, USA. Available online at http://www.epa.gov/opptintr/exposure/pubs/episuitedl.htm as of June 12, 2012. 3http://www.islechem.com/pdfs/1000msds.pdf 4 ------- U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 2. General Information on Exposure 2.1 Production Volume and Use Pattern 3,4-dichloro-d,d,d-trifluorotoluene had an aggregated production and/or import volume in the United States less than 500,000 pounds during calendar year 2005. No industrial processing and uses, and commercial and consumer uses were reported for the chemical. 2.2 Environmental Exposure and Fate 3,4-dichloro-d,d,d-trifluorotoluene is expected to have moderate mobility in soil. 3,4-dichloro- d,d,d-trifluorotoluene achieved 0% degradation over the course of a 28-day incubation period using a mixed microbial inoculum isolated from a fertile garden during a closed bottle (OTS 796.3200) test; it is considered not readily biodegradable. Volatilization of 3,4-dichloro- d,d,d-trifluorotoluene is high based on the Henry's Law constant. The rate of hydrolysis is expected to be negligible. The rate of atmospheric photooxidation is negligible. 3,4-dichloro- d,d,d-trifluorotoluene is expected to have high persistence (P3) and moderate bioaccumulation potential (B2). The environmental fate properties of 3,4-dichloro-d,d,d-trifluorotoluene are provided in Table 2. Table 2. Environmental Fate Characteristics of 3,4-dichloro-d,d,a-trifluorotoluene [CI name: Benzene,1,2 dichloro 4 (trifluoromethyl)l1 Property Value CASRN 328-84-7 Photodegradation Half-life 133 days (estimated)2 Hydrolysis Half-life Stable Biodegradation 0% after 28 days (not readily biodegradable; OTS 796.3200) Bioaccumulation Factor BAF = 2,168 (estimated)2; BCF = 1,500 (measured in rainbow trout)3 Log Koc 3.4 (estimated)2 Fugacity (Level III Model)2 Air (%) Water (%) Soil (%) Sediment (%) 31.1 31.3 31.8 5.8 Persistence4 P3 (high) Bioaccumulation4 B2 (moderate) 'The Dow Chemical Company. 2005. Test Plan and Robust Summary for 3,4-Dichloro-alpha, alpha, alpha- trifluorotoluene. Available online at http://www.epa.gov/chemrtk/pubs/summaries/34dichlo/cl5026tc.litm as of June 12, 2012. 5 ------- U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 2U.S. EPA. 2012. Estimation Programs Interface Suite™ for Microsoft® Windows, v4.10. U.S. Environmental Protection Agency, Washington, DC, USA. Available online at http://www.epa.gov/opptintr/exposure/pubs/episuitedl.htm as of June 12, 2012. 3HSDB. 2012. 3,4-Dichlorobenzotrifluoride CASRN 328-84-7. Available online at http://toxnet.nlm.nih.gov/cgi- bin/sis/htmlgen?HSDB as of June 13, 2012. 4Federal Register. 1999. Category for Persistent, Bioaccumulative, and Toxic New Chemical Substances. Federal Register 64, Number 213 (November 4, 1999) pp. 60194-60204. Conclusion: 3,4-dichloro-d,d,d-trifluorotoluene is a colorless liquid with high vapor pressure and moderate water solubility. It is expected to have moderate mobility in soil. Volatilization of 3,4-dichloro-d,d,d-trifluorotoluene is high based on its Henry's Law constant. The rate of hydrolysis is expected to be negligible. The rate of atmospheric photooxidation is negligible. 3,4-dichloro-d,d,d-trifluorotoluene is not readily biodegradable. 3,4-dichloro- d,d,d-trifluorotoluene is expected to have high persistence (P3) and moderate bioaccumulation potential (B2). 3. Human Health Hazard A summary of human health hazard data submitted for SIDS endpoints is provided in Table 3. Acute Oral Toxicity (1) CF Nelson rats (10 males/dose) were administered 3,4-dichloro-d,d,d-trifluorotoluene (purity not specified) via gavage at 240, 470, 940 or 1870 mg/kg and observed for up to 14 days after administration. Mortality occurred in 3 of 10 animals at 940 mg/kg and 9 of 10 animals at 1870 mg/kg-bw. LDso = 1150 mg/kg (2) Sprague-Dawley rats (7/sex/dose) were administered undiluted 3,4-dichloro-d,d,a- trifluorotoluene (purity not specified) via gavage at 1.281, 1.658, 2.034, 2.630, 3.229, 5.126 or 8.137 mL/kg (~ 1893, 2451, 3006, 3887, 4772, 7576 and 12,026 mg/kg) and observed for up to 14 days after dose administration. Mortality was observed at doses > 1.281 mL/kg and 100% mortality was noted at doses > 3.229 mL/kg. LD50 ~ 3288 mg/kg (males) LD50 ~ 2896 mg/kg (females) Acute Inhalation Toxicity Sprague-Dawley rats (5/sex/dose) were exposed (whole-body) to 3,4-dichloro-d,d,a- trifluorotoluene vapor (purity not specified) at nominal concentrations of 8.59 or 15.86 mg/L for 4 hours and observed for up to 14 days post-exposure. No mortality occurred. 4-h LC50 > ~ 15.86 mg/L 6 ------- U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 Acute Dermal Toxicity (1) Three female rabbits were administered undiluted 3,4-dichloro-d,d,d-trifluorotoluene (purity not specified) dermally at 5000 mg/kg under occlusive conditions and observed for up to 14 days after administration. Strain, duration of exposure, and further study details were not provided. One animal was sacrificed 12 days after dosing. LDso > 5000 mg/kg (2) Five male albino rabbits were administered undiluted 3,4-dichloro-d,d,d-trifluorotoluene (purity not specified) dermally at 5000 mg/kg-bw to shaven skin under occluded conditions for 24 hours. Animals were observed for up to 14 days after administration. One death occurred within 5 days of administration. LD50 > 5000 mg/kg Repeated-Dose Toxicity In a modified oral gavage repeated-dose/reproductive/developmental toxicity screening test, Sprague-Dawley rats were administered 3,4-dichloro-d,d,d-trifluorotoluene (95% purity) at 2, 5, 15 and 45 mg/kg-day for 4 weeks premating through one reproduction period (76 - 83 days) until Fi litters were weaned and Fi weanling animals were dosed for at least 90 days. Weekly body weight and feed consumption, clinical pathology, urinalysis and gross necropsy were evaluated on Fo animals. Gross and microscopic histopathological evaluations of organs and tissues (unspecified) were performed on all Fi rats. Increased absolute and relative liver and kidney weights occurred at 15 mg/kg-day, but there were no histopathologic changes. NOAEL (systemic toxicity) = 45 mg/kg-day (highest dose tested) Reproductive/Developmental Toxicity In the modified repeated-dose/reproductive/developmental toxicity screening test described previously, data for specific reproductive/developmental toxicity parameters that may have been examined (e.g., fertility, number of live pups per litter) were not provided in the robust summaries. NOAEL (reproductive/developmental toxicity) = 45 mg/kg-day (highest dose tested) Genetic Toxicity — Gene Mutations In vitro Salmonella typhimurium strains TA1535, TA1537, TA1538, TA98 and TA100, Saccharomyces cerevisiae strain D4 and Escherichia coli strain W3110/pol A were exposed to 3,4-dichloro- d,d,d-trifluorotoluene at concentrations of 0.01, 0.1, 1, 5 or 10 |iL/plate in the presence and absence of metabolic activation. 3,4-Dichloro-d,d,d-trifluorotoluene was toxic to all strains except S. cerevisiae D4 at 10 |iL/ plate and toxic to S. typhimurium strains TA1537, TA1538 and 7 ------- U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 TA100 at 5 |iL/plate. Negative and positive controls were tested concurrently. No mutagenic activity was noted. 3,4-Dichloro-d,d,d-trifluorotoluene did not induce gene mutations in this assay. Genetic Toxicity — Chromosomal Aberrations No adequate data submitted. Genetic Toxicity — Other In vitro Mouse lymphoma cells (L5178Y) were exposed to 3,4-dichloro-d,d,d-trifluorotoluene at concentrations of 0.625, 1.259, 2.5, 5 or 20 |iL/mL in the presence and absence of metabolic activation. Cytotoxic concentrations were 1.25, 2.5, 5 and 10 |iL/mL. Positive and negative controls were tested concurrently and responded appropriately. The sister chromatid exchange (SCE) frequency increased consistently with dose in the presence of metabolic activation. In the absence of activation, 3,4-dichloro-d,d,d-trifluorotoluene only slightly induced an increase in SCE frequency. 3,4-Dichloro-d,d,d-trifluorotoluene induced sister chromatid exchange in the presence of metabolic activation but only weakly in the absence of metabolic activation. Additional Information Skin Irritation Undiluted 3,4-dichloro-d,d,d-trifluorotoluene (0.5 mL, purity not specified) was applied to one intact and one abraded site of six rabbits (strain and sex not specified) under occlusive conditions for 24 hours and assessed for up to 72 hours following dose administration. The mean value for intact and abraded skin at 24 and 72 hours were all 2.0 for erythema. The mean values for intact and abraded skin at 24 hours for edema were 4 and 4, respectively, and the mean values for intact and abraded skin at 72 hours for edema were 0 and 1.3, respectively. The overall primary irritation index was 4.3. The duration was longer than current standard irritation studies (24 vs. 4 hrs). 3,4-Dichloro-d,d,d-trifluorotoluene was moderately irritating to rabbit skin in this study. Eye Irritation (1) Undiluted 3,4-dichloro-d,d,d-trifluorotoluene (0.1 mL, purity not specified) was instilled in the conjunctival sac of the eyes of six rabbits (strain and sex not specified). Test eyes were washed after 24 hours of exposure. Animals were observed for up to 7 days after administration. No ocular irritation was noted at day 7. Scores ranged from 0.7 to 3.3 during the first three days. 3,4-Dichloro-d,d,d-trifluorotoluene was slightly irritating to rabbit eyes in this study. 8 ------- U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 (2) Undiluted 3,4-dichloro-d,d,d-trifluorotoluene (0.1 mL, purity not specified) was instilled in the conjunctival sac of the eyes of six rabbits (strain and sex not specified). Test eyes were washed after 24 hours of exposure and scored at 24, 48 and 72 hours post-dosing. Effects were limited to conjunctival effects with a mean value of 0.3. No ocular irritation was noted at 72 hours. 3,4-Dichloro-d,d,d-trifluorotoluene was slightly irritating to rabbit eyes in this study. Sensitization In a Buehler test, guinea pigs (12 males, strain not specified) were administered nine sensitizing topical applications of 3,4-dichloro-d,d,d-trifluorotoluene in ethyl alcohol at 10% by weight (w/v) under occluded conditions 6 hours/day over a 21-day period. Fourteen days after induction applications, animals were administered two challenge applications (details not specified). Slight irritation was noted in seven animals on the second induction day only and in one or two animals during the remainder of the induction period. 3,4-Dichloro-d,d,d-trifluorotoluene was not sensitizing in guinea pigs in this study. Conclusion: Acute oral and dermal toxicity of 3,4-dichloro-d,d,d-trifluorotoluene is low in rats and rabbits, respectively. Acute inhalation toxicity in rats is moderate. In a modified oral gavage repeated-dose/ reproductive/developmental toxicity study in rats no systemic effects were observed up to 45 mg/kg-day; the NOAEL for systemic toxicity is 45 mg/kg-day, the highest dose tested. No effects on reproductive or developmental parameters were reported; the reproductive and developmental NOAEL is 45 mg/kg-day, the highest dose tested. 3,4- Dichloro-d,d,d-trifluorotoluene was not mutagenic in bacteria or mammalian cells in vitro. No data are available for chromosomal aberrations. 3,4-Dichloro-d,d,d-trifluorotoluene induced sister chromatid exchange (SCEs) in mouse lymphoma L5178Y cells in the presence of metabolic activation, but weakly induced SCE in the absence of activation. 3,4-Dichloro-d,d,a- trifluorotoluene is irritating to rabbit skin, is not irritating to rabbit eyes and is not a dermal sensitizer in guinea pigs. 9 ------- U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 Table 3. Summary Table of the Screening Information Data Set as Submitted under the U.S. HPV Challenge Program - Human Health Data Endpoint 3,4-Dichloro-d,d,d-trifluorotoluene (328-84-7) Acute Oral Toxicity LD50 (mg/kg) 1150 Acute Inhalation Toxicity LC50 (mg/L) > 15.86 Acute Dermal Toxicity LD50 (mg/kg) >5000 Repeated-Dose Toxicity NOAEL/LOAEL Oral (mg/kg-day) NOAEL = 45 (highest dose tested) Reproductive Toxicity NOAEL/LOAEL Oral (mg/kg-day) NOAEL = 45 (highest dose tested) Developmental Toxicity NOAEL/LOAEL Oral (mg/kg-day) NOAEL = 45 (highest dose tested) Genetic Toxicity - Gene Mutation In vitro Negative Genetic Toxicity - Chromosomal Aberrations In vitro No Data Genetic Toxicity - Other SCE Positive Additional Information Skin Irritation Eye Irritation Skin Sensitization Irritating Irritating Negative Measured data in bold 4. Hazard to the Environment A summary of aquatic toxicity data submitted for SIDS endpoints is provided in Table 4. Acute Toxicity to Fish (1) Fathead minnows (Pimephalespromelas) were exposed to 3,4-dichloro-a,a,a- trifluorotoluene at nominal concentrations of 0, 1.8, 2.7, 4.2, 6.5 or 10 mg/L under flow-through conditions for 96 hours. Measured concentrations were 0, 0.6, 0.9, 1.5, 2.2 and 3.5 mg/L. There was 100% mortality at the highest concentration tested. 96-h LCso = 2.3 mg/L 10 ------- U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 (2) Rainbow trout (Oncorhynchus mykiss) were exposed to 3,4-dichloro-d,d,d-trifluorotoluene at nominal concentrations of 0, 0.89, 1.4, 2.1, 3.2 or 5 mg/L under flow-through conditions for 96 hours. Measured concentrations were 0, 0.34, 0.52, 0.83, 1.4 and 3.4 mg/L. At 96 hours, 20 and 100% mortality occurred at 1.2 and 3.4 mg/L, respectively. 96-h LCso = 2.05 mg/L Acute Toxicity to Aquatic Invertebrates Gammarids (Gammarus fasciatus) were exposed to 3,4-dichloro-d,d,d-trifluorotoluene at nominal concentrations of 0, 0.89, 1.4, 2.1, 3.3 or 5 mg/L under flow-through conditions for 96 hours. Measured concentrations were 0, 0.52, 0.83, 1.3, 1.9 and 2.8 mg/L. At 96 hours, 70 and 100% mortality occurred at the two highest concentrations tested. 96-h LCso = 1.7 mg/L Toxicity to Aquatic Plants Green algae (Pseudokirchneriella subcapitata) were exposed to 3,4-dichloro-d,d,a- trifluorotoluene at nominal concentrations of 0, 3.1, 6.3, 13, 25 or 50 mg/L for 96 hours. Measured concentrations were 0, 0.40, 0.62, 1.8, 3.7 and 8.6 mg/L. Algae growth/cell density was not inhibited at any of the test concentrations compared to the controls. 96-h LCso > 8.6 mg/L (highest concentration tested) Chronic Toxicity to Fish Rainbow trout (O. mykiss) were exposed to 3,4-dichloro-d,d,d-trifluorotoluene at nominal concentrations of 0, 0.034, 0.068, 0.13, 0.25 or 0.51 mg/L under static conditions for 89 days (60 days post-hatch). Measured concentrations were not specified. Parameters tested included embryo viability, survival of organism at hatch and survival and growth post-hatch. No effects on embryo viability or hatchability were noted at any concentration. At the two highest concentrations, an adverse effect on mean total length was noted. The only reduction in weight was noted at the highest concentration tested. The maximum acceptable toxicant concentration was calculated to be 0.18 mg/L. LOEC = 0.25 mg/L (based on decreased mean total length) NOEC = 0.13 mg/L Chronic Toxicity to Invertebrates Water fleas (Daphnia magna) were exposed to 3,4-dichloro-d,d,d-trifluorotoluene at mean measured concentrations of 0, 0.03, 0.06, 0.12, 0.24 or 0.38 mg/L for 21 days. Mortality was markedly increased at all concentrations except 0.03 mg/L. At 0.24 mg/L, the organisms produced fewer young and those treated at 0.38 mg/L didn't produce any young. 11 ------- U.S. Environmental Protection Agency Hazard Characterization Document September, 2014 21-d LCso = 0.16 mg/L LOEC = 0.06 mg/L (based on mortality) NOEC = 0.03 mg/L Conclusion: The 96-h LCso for fish and aquatic invertebrates from 3,4-dichloro-d,d,a- trifluorotoluene is 2.05-2.3 and 1.7 mg/L, respectively. The 96-h ECso to aquatic plants for growth rate is > 8.6 mg/L. The 21-d LCso for chronic aquatic invertebrates from 3,4-dichloro- d,d,d-trifluorotoluene is 0.16 mg/L. Table 4. Summary of the Screening Information Data Set as Submitted under the U.S. HPV Challenge Program- Aquatic Toxicity Endpoints 3,4-Dichloro-d,d,d-benzotrifluoride (328-84-7) Fish 96-h LCso (mg/L) 2.05-2.3 Aquatic Invertebrates 96-h LCso (mg/L) 1.7 Aquatic Plants 96-h EgC5o(mg/L) >8.6 Chronic Aquatic Invertebrates 21-d LCso (mg/L) 0.16 Bold = experimental data (i.e., derived from testing). 12 ------- |