U.S. Environmental Protection Agency
Hazard Characterization Document
September, 2014
SCREENING-LEVEL HAZARD CHARACTERIZATION
3,4-Dichloro-&,&,&-trifluorotoluene
(CASRN 328-84-7)
The High Production Volume (HPV) Challenge Program1 was conceived as a voluntary initiative
aimed at developing and making publicly available screening-level health and environmental
effects information on chemicals manufactured in or imported into the United States in quantities
greater than one million pounds per year. In the Challenge Program, producers and importers of
HPV chemicals voluntarily sponsored chemicals; sponsorship entailed the identification and
initial assessment of the adequacy of existing toxicity data/information, conducting new testing if
adequate data did not exist, and making both new and existing data and information available to
the public. Each complete data submission contains data on 18 internationally agreed to "SIDS"
(Screening Information Data Set1'2) endpoints that are screening-level indicators of potential
hazards (toxicity) for humans or the environment.
The Environmental Protection Agency's Office of Pollution Prevention and Toxics (OPPT) is
evaluating the data submitted in the HPV Challenge Program on approximately 1400 sponsored
chemicals by developing hazard characterizations (HCs). These HCs consist of an evaluation of
the quality and completeness of the data set provided in the Challenge Program submissions.
They are not intended to be definitive statements regarding the possibility of unreasonable risk of
injury to health or the environment.
The evaluation is performed according to established EPA guidance2'3 and is based primarily on
hazard data provided by sponsors; however, in preparing the hazard characterization, EPA
considered its own comments and public comments on the original submission as well as the
sponsor's responses to comments and revisions made to the submission. In order to determine
whether any new hazard information was developed since the time of the HPV submission, a
search of the following databases was made from one year prior to the date of the HPV
Challenge submission to the present: (ChemID to locate available data sources including
Medline/PubMed, Toxline, HSDB, IRIS, NTP, AT SDR, IARC, EXTOXNET, EPA SRS, etc.),
STN/CAS online databases (Registry file for locators, ChemAbs for toxicology data, RTECS,
Merck, etc.), Science Direct and ECHA4. OPPT's focus on these specific sources is based on
their being of high quality, highly relevant to hazard characterization, and publicly available.
OPPT does not develop HCs for those HPV chemicals which have already been assessed
internationally through the HPV program of the Organization for Economic Cooperation and
Development (OECD) and for which Screening Initial Data Set (SIDS) Initial Assessment
Reports (SIAR) and SIDS Initial Assessment Profiles (SIAP) are available. These documents are
presented in an international forum that involves review and endorsement by governmental
authorities around the world. OPPT is an active participant in these meetings and accepts these
documents as reliable screening-level hazard assessments.
1	U.S. EPA. High Production Volume (HPV) Challenge Program; http://www.epa.gov/chemrtk/index.htm.
2	U.S. EPA. HPV Challenge Program - Information Sources; http://www.epa.gov/chemrtk/pubs/general/guidocs.htm.
3	U.S. EPA. Risk Assessment Guidelines; http://cfpub.epa.gov/ncea/raf/rafguid.cfm.
4	European Chemicals Agency, http://echa.europa.eu.

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Hazard Characterization Document
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These hazard characterizations are technical documents intended to inform subsequent decisions
and actions by OPPT. Accordingly, the documents are not written with the goal of informing the
general public. However, they do provide a vehicle for public access to a concise assessment of
the raw technical data on HPV chemicals and provide information previously not readily
available to the public.
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Chemical Abstract
Service Registry Number	328-84-7
	(CASRN)	
Chemical Abstract Index
Name	Benzene, l,2-dichloro-4-(trifluoromethyl)-
Structural Formula
F
	SMILES: FC(F)(F)c(ccc(c 1 Cl)Cl)c 1	
Summary
3,4-Dichloro-d,d,d-trifluorotoluene is a colorless liquid with high vapor pressure and moderate
water solubility. It is expected to have moderate mobility in soil. Volatilization of 3,4-
dichloro-d,d,d-trifluorotoluene is high based on its Henry's Law constant. The rate of
hydrolysis is expected to be negligible. The rate of atmospheric photooxidation is negligible.
3.4-Dichloro-d,d,d-trifluorotoluene	is not readily biodegradable. 3,4-Dichloro-d,d,a-
trifluorotoluene is expected to have high persistence (P3) and moderate bioaccumulation
potential (B2).
Acute oral and dermal toxicity of 3,4-dichloro-d,d,d-trifluorotoluene is low in rats and rabbits,
respectively. Acute inhalation toxicity in rats is moderate. In a modified oral gavage repeated-
dose/ reproductive/developmental toxicity study in rats no systemic effects were observed up
to 45 mg/kg-day; the NOAEL for systemic toxicity is 45 mg/kg-day, the highest dose tested.
No effects on reproductive or developmental parameters were reported; the reproductive and
developmental NOAEL is 45 mg/kg-day, the highest dose tested. 3,4-Dichloro-d,d,a-
trifluorotoluene was not mutagenic in bacteria or mammalian cells in vitro. No data are
available for chromosomal aberrations. 3,4-Dichloro-d,d,d-trifluorotoluene induced sister
chromatid exchange (SCEs) in mouse lymphoma L5178Y cells in the presence of metabolic
activation, but weakly induced SCE in the absence of activation. 3,4-Dichloro-d,d,a-
trifluorotoluene is irritating to rabbit skin, is not irritating to rabbit eyes and is not a dermal
sensitizer in guinea pigs.
The 96-h LCso for fish and aquatic invertebrates from 3,4-dichloro-d,d,d-trifluorotoluene is
2.05-2.3	and 1.7 mg/L, respectively. The 96-h ECsoto aquatic plants for growth rate is > 8.6
mg/L. The 21-d LCso for chronic aquatic invertebrates from 3,4-dichloro-d,d,a-
trifluorotoluene is 0.16 mg/L.
The genetic toxicity (chromosomal aberrations) endpoint was identified as a data gap under
the HPV Chemical Challenge Program.
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The sponsor, Dow AgroSciences LLC, submitted a Test Plan and Robust Summaries to EPA for
3,4-dichloro-d,d,d-trifluorotoluene (CASRN 328-84-7; 9th CI name: benzene, l,2-dichloro-4-
(trifluoromethyl)-) on December 18, 2003. EPA posted the submission on the ChemRTK HPV
Challenge website on March 5, 2004
(http://www.epa.gov/chemrtk/pubs/summaries/34dichlo/cl5026tc.htm). EPA comments on the
original submission were posted to the website on July 26, 2004. Public comments were also
received and posted to the website. The sponsor submitted updated/revised documents on
August 25, 2005, which were posted to the ChemRTK website on October 6, 2005.
1. Chemical Identity
1.1	Identification and Purity
3,4-dichloro-d,d,d-trifluorotoluene is a colorless liquid with high vapor pressure and moderate
water solubility and is an intermediate in the production of pesticides. The purity is reported as
95 to 99.5% when provided in the robust summaries.
1.2	Physical-Chemical Properties
The physical-chemical properties of 3,4-dichloro-d,d,d-trifluorotoluene are summarized in Table
1.
Table 1. Physical-Chemical Properties of 3,4-dichloro-a,a,d-trifluorotoluene
(CI name: Benzene, 1,2 dichloro 4 (trifluoromethyl)1
Property
Value
CASRN
328-84-7
Molecular Weight
215.0
Physical State
Colorless liquid
Melting Point
-12°C (measured)
Boiling Point
173.5°C (measured)
Vapor Pressure
1.6 mm Hg at 20°C (measured)
Dissociation Constant
(pKa)
Not applicable
Henry's Law Constant
2.9x 10"2 atm-m3/mole (estimated)2
Water Solubility
11.6 mg/L at 23°C (measured)
Log Kow
3.843
'The Dow Chemical Company. 2005. Test Plan and Robust Summary for 3,4-Dichloro-alpha, alpha, alpha-
trifluorotoluene. Available online at http://www.epa.gov/chemrtk/pubs/summaries/34dichlo/cl5026tc.htm as of
June 12, 2012.
2U.S. EPA. 2012. Estimation Programs Interface Suite™ for Microsoft® Windows, v4.10. U.S. Environmental
Protection Agency, Washington, DC, USA. Available online at
http://www.epa.gov/opptintr/exposure/pubs/episuitedl.htm as of June 12, 2012.
3http://www.islechem.com/pdfs/1000msds.pdf
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2. General Information on Exposure
2.1	Production Volume and Use Pattern
3,4-dichloro-d,d,d-trifluorotoluene had an aggregated production and/or import volume in the
United States less than 500,000 pounds during calendar year 2005.
No industrial processing and uses, and commercial and consumer uses were reported for the
chemical.
2.2	Environmental Exposure and Fate
3,4-dichloro-d,d,d-trifluorotoluene is expected to have moderate mobility in soil. 3,4-dichloro-
d,d,d-trifluorotoluene achieved 0% degradation over the course of a 28-day incubation period
using a mixed microbial inoculum isolated from a fertile garden during a closed bottle (OTS
796.3200) test; it is considered not readily biodegradable. Volatilization of 3,4-dichloro-
d,d,d-trifluorotoluene is high based on the Henry's Law constant. The rate of hydrolysis is
expected to be negligible. The rate of atmospheric photooxidation is negligible. 3,4-dichloro-
d,d,d-trifluorotoluene is expected to have high persistence (P3) and moderate bioaccumulation
potential (B2). The environmental fate properties of 3,4-dichloro-d,d,d-trifluorotoluene are
provided in Table 2.
Table 2. Environmental Fate Characteristics of 3,4-dichloro-d,d,a-trifluorotoluene
[CI name: Benzene,1,2 dichloro 4 (trifluoromethyl)l1
Property
Value
CASRN
328-84-7
Photodegradation Half-life
133 days (estimated)2
Hydrolysis Half-life
Stable
Biodegradation
0% after 28 days (not readily biodegradable; OTS 796.3200)
Bioaccumulation Factor
BAF = 2,168 (estimated)2;
BCF = 1,500 (measured in rainbow trout)3
Log Koc
3.4 (estimated)2
Fugacity
(Level III Model)2
Air (%)
Water (%)
Soil (%)
Sediment (%)
31.1
31.3
31.8
5.8
Persistence4
P3 (high)
Bioaccumulation4
B2 (moderate)
'The Dow Chemical Company. 2005. Test Plan and Robust Summary for 3,4-Dichloro-alpha, alpha, alpha-
trifluorotoluene. Available online at http://www.epa.gov/chemrtk/pubs/summaries/34dichlo/cl5026tc.litm as of
June 12, 2012.
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2U.S. EPA. 2012. Estimation Programs Interface Suite™ for Microsoft® Windows, v4.10. U.S. Environmental
Protection Agency, Washington, DC, USA. Available online at
http://www.epa.gov/opptintr/exposure/pubs/episuitedl.htm as of June 12, 2012.
3HSDB. 2012. 3,4-Dichlorobenzotrifluoride CASRN 328-84-7. Available online at http://toxnet.nlm.nih.gov/cgi-
bin/sis/htmlgen?HSDB as of June 13, 2012.
4Federal Register. 1999. Category for Persistent, Bioaccumulative, and Toxic New Chemical Substances. Federal
Register 64, Number 213 (November 4, 1999) pp. 60194-60204.
Conclusion: 3,4-dichloro-d,d,d-trifluorotoluene is a colorless liquid with high vapor pressure
and moderate water solubility. It is expected to have moderate mobility in soil. Volatilization of
3,4-dichloro-d,d,d-trifluorotoluene is high based on its Henry's Law constant. The rate of
hydrolysis is expected to be negligible. The rate of atmospheric photooxidation is negligible.
3,4-dichloro-d,d,d-trifluorotoluene is not readily biodegradable. 3,4-dichloro-
d,d,d-trifluorotoluene is expected to have high persistence (P3) and moderate bioaccumulation
potential (B2).
3. Human Health Hazard
A summary of human health hazard data submitted for SIDS endpoints is provided in Table 3.
Acute Oral Toxicity
(1)	CF Nelson rats (10 males/dose) were administered 3,4-dichloro-d,d,d-trifluorotoluene (purity
not specified) via gavage at 240, 470, 940 or 1870 mg/kg and observed for up to 14 days after
administration. Mortality occurred in 3 of 10 animals at 940 mg/kg and 9 of 10 animals at 1870
mg/kg-bw.
LDso = 1150 mg/kg
(2)	Sprague-Dawley rats (7/sex/dose) were administered undiluted 3,4-dichloro-d,d,a-
trifluorotoluene (purity not specified) via gavage at 1.281, 1.658, 2.034, 2.630, 3.229, 5.126 or
8.137 mL/kg (~ 1893, 2451, 3006, 3887, 4772, 7576 and 12,026 mg/kg) and observed for up to
14 days after dose administration. Mortality was observed at doses > 1.281 mL/kg and 100%
mortality was noted at doses > 3.229 mL/kg.
LD50 ~ 3288 mg/kg (males)
LD50 ~ 2896 mg/kg (females)
Acute Inhalation Toxicity
Sprague-Dawley rats (5/sex/dose) were exposed (whole-body) to 3,4-dichloro-d,d,a-
trifluorotoluene vapor (purity not specified) at nominal concentrations of 8.59 or 15.86 mg/L for
4 hours and observed for up to 14 days post-exposure. No mortality occurred.
4-h LC50 > ~ 15.86 mg/L
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Acute Dermal Toxicity
(1)	Three female rabbits were administered undiluted 3,4-dichloro-d,d,d-trifluorotoluene (purity
not specified) dermally at 5000 mg/kg under occlusive conditions and observed for up to 14 days
after administration. Strain, duration of exposure, and further study details were not provided.
One animal was sacrificed 12 days after dosing.
LDso > 5000 mg/kg
(2)	Five male albino rabbits were administered undiluted 3,4-dichloro-d,d,d-trifluorotoluene
(purity not specified) dermally at 5000 mg/kg-bw to shaven skin under occluded conditions for
24 hours. Animals were observed for up to 14 days after administration. One death occurred
within 5 days of administration.
LD50 > 5000 mg/kg
Repeated-Dose Toxicity
In a modified oral gavage repeated-dose/reproductive/developmental toxicity screening test,
Sprague-Dawley rats were administered 3,4-dichloro-d,d,d-trifluorotoluene (95% purity) at 2, 5,
15 and 45 mg/kg-day for 4 weeks premating through one reproduction period (76 - 83 days)
until Fi litters were weaned and Fi weanling animals were dosed for at least 90 days. Weekly
body weight and feed consumption, clinical pathology, urinalysis and gross necropsy were
evaluated on Fo animals. Gross and microscopic histopathological evaluations of organs and
tissues (unspecified) were performed on all Fi rats. Increased absolute and relative liver and
kidney weights occurred at 15 mg/kg-day, but there were no histopathologic changes.
NOAEL (systemic toxicity) = 45 mg/kg-day (highest dose tested)
Reproductive/Developmental Toxicity
In the modified repeated-dose/reproductive/developmental toxicity screening test described
previously, data for specific reproductive/developmental toxicity parameters that may have been
examined (e.g., fertility, number of live pups per litter) were not provided in the robust
summaries.
NOAEL (reproductive/developmental toxicity) = 45 mg/kg-day (highest dose tested)
Genetic Toxicity — Gene Mutations
In vitro
Salmonella typhimurium strains TA1535, TA1537, TA1538, TA98 and TA100, Saccharomyces
cerevisiae strain D4 and Escherichia coli strain W3110/pol A were exposed to 3,4-dichloro-
d,d,d-trifluorotoluene at concentrations of 0.01, 0.1, 1, 5 or 10 |iL/plate in the presence and
absence of metabolic activation. 3,4-Dichloro-d,d,d-trifluorotoluene was toxic to all strains
except S. cerevisiae D4 at 10 |iL/ plate and toxic to S. typhimurium strains TA1537, TA1538 and
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TA100 at 5 |iL/plate. Negative and positive controls were tested concurrently. No mutagenic
activity was noted.
3,4-Dichloro-d,d,d-trifluorotoluene did not induce gene mutations in this assay.
Genetic Toxicity — Chromosomal Aberrations
No adequate data submitted.
Genetic Toxicity — Other
In vitro
Mouse lymphoma cells (L5178Y) were exposed to 3,4-dichloro-d,d,d-trifluorotoluene at
concentrations of 0.625, 1.259, 2.5, 5 or 20 |iL/mL in the presence and absence of metabolic
activation. Cytotoxic concentrations were 1.25, 2.5, 5 and 10 |iL/mL. Positive and negative
controls were tested concurrently and responded appropriately. The sister chromatid exchange
(SCE) frequency increased consistently with dose in the presence of metabolic activation. In the
absence of activation, 3,4-dichloro-d,d,d-trifluorotoluene only slightly induced an increase in
SCE frequency.
3,4-Dichloro-d,d,d-trifluorotoluene induced sister chromatid exchange in the presence of
metabolic activation but only weakly in the absence of metabolic activation.
Additional Information
Skin Irritation
Undiluted 3,4-dichloro-d,d,d-trifluorotoluene (0.5 mL, purity not specified) was applied to one
intact and one abraded site of six rabbits (strain and sex not specified) under occlusive conditions
for 24 hours and assessed for up to 72 hours following dose administration. The mean value for
intact and abraded skin at 24 and 72 hours were all 2.0 for erythema. The mean values for intact
and abraded skin at 24 hours for edema were 4 and 4, respectively, and the mean values for intact
and abraded skin at 72 hours for edema were 0 and 1.3, respectively. The overall primary
irritation index was 4.3. The duration was longer than current standard irritation studies (24 vs.
4 hrs).
3,4-Dichloro-d,d,d-trifluorotoluene was moderately irritating to rabbit skin in this study.
Eye Irritation
(1) Undiluted 3,4-dichloro-d,d,d-trifluorotoluene (0.1 mL, purity not specified) was instilled in
the conjunctival sac of the eyes of six rabbits (strain and sex not specified). Test eyes were
washed after 24 hours of exposure. Animals were observed for up to 7 days after administration.
No ocular irritation was noted at day 7. Scores ranged from 0.7 to 3.3 during the first three days.
3,4-Dichloro-d,d,d-trifluorotoluene was slightly irritating to rabbit eyes in this study.
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(2) Undiluted 3,4-dichloro-d,d,d-trifluorotoluene (0.1 mL, purity not specified) was instilled in
the conjunctival sac of the eyes of six rabbits (strain and sex not specified). Test eyes were
washed after 24 hours of exposure and scored at 24, 48 and 72 hours post-dosing. Effects were
limited to conjunctival effects with a mean value of 0.3. No ocular irritation was noted at 72
hours.
3,4-Dichloro-d,d,d-trifluorotoluene was slightly irritating to rabbit eyes in this study.
Sensitization
In a Buehler test, guinea pigs (12 males, strain not specified) were administered nine sensitizing
topical applications of 3,4-dichloro-d,d,d-trifluorotoluene in ethyl alcohol at 10% by weight
(w/v) under occluded conditions 6 hours/day over a 21-day period. Fourteen days after induction
applications, animals were administered two challenge applications (details not specified).
Slight irritation was noted in seven animals on the second induction day only and in one or two
animals during the remainder of the induction period.
3,4-Dichloro-d,d,d-trifluorotoluene was not sensitizing in guinea pigs in this study.
Conclusion: Acute oral and dermal toxicity of 3,4-dichloro-d,d,d-trifluorotoluene is low in rats
and rabbits, respectively. Acute inhalation toxicity in rats is moderate. In a modified oral gavage
repeated-dose/ reproductive/developmental toxicity study in rats no systemic effects were
observed up to 45 mg/kg-day; the NOAEL for systemic toxicity is 45 mg/kg-day, the highest
dose tested. No effects on reproductive or developmental parameters were reported; the
reproductive and developmental NOAEL is 45 mg/kg-day, the highest dose tested. 3,4-
Dichloro-d,d,d-trifluorotoluene was not mutagenic in bacteria or mammalian cells in vitro. No
data are available for chromosomal aberrations. 3,4-Dichloro-d,d,d-trifluorotoluene induced
sister chromatid exchange (SCEs) in mouse lymphoma L5178Y cells in the presence of
metabolic activation, but weakly induced SCE in the absence of activation. 3,4-Dichloro-d,d,a-
trifluorotoluene is irritating to rabbit skin, is not irritating to rabbit eyes and is not a dermal
sensitizer in guinea pigs.
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Table 3. Summary Table of the Screening Information Data Set
as Submitted under the U.S. HPV Challenge Program -
Human Health Data
Endpoint
3,4-Dichloro-d,d,d-trifluorotoluene
(328-84-7)
Acute Oral Toxicity
LD50 (mg/kg)
1150
Acute Inhalation Toxicity
LC50 (mg/L)
> 15.86
Acute Dermal Toxicity
LD50 (mg/kg)
>5000
Repeated-Dose Toxicity
NOAEL/LOAEL
Oral (mg/kg-day)
NOAEL = 45
(highest dose tested)
Reproductive Toxicity
NOAEL/LOAEL
Oral (mg/kg-day)
NOAEL = 45
(highest dose tested)
Developmental Toxicity
NOAEL/LOAEL
Oral (mg/kg-day)
NOAEL = 45
(highest dose tested)
Genetic Toxicity - Gene Mutation
In vitro
Negative
Genetic Toxicity - Chromosomal
Aberrations In vitro
No Data
Genetic Toxicity - Other
SCE
Positive
Additional Information
Skin Irritation
Eye Irritation
Skin Sensitization
Irritating
Irritating
Negative
Measured data in bold
4. Hazard to the Environment
A summary of aquatic toxicity data submitted for SIDS endpoints is provided in Table 4.
Acute Toxicity to Fish
(1) Fathead minnows (Pimephalespromelas) were exposed to 3,4-dichloro-a,a,a-
trifluorotoluene at nominal concentrations of 0, 1.8, 2.7, 4.2, 6.5 or 10 mg/L under flow-through
conditions for 96 hours. Measured concentrations were 0, 0.6, 0.9, 1.5, 2.2 and 3.5 mg/L. There
was 100% mortality at the highest concentration tested.
96-h LCso = 2.3 mg/L
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(2) Rainbow trout (Oncorhynchus mykiss) were exposed to 3,4-dichloro-d,d,d-trifluorotoluene at
nominal concentrations of 0, 0.89, 1.4, 2.1, 3.2 or 5 mg/L under flow-through conditions for 96
hours. Measured concentrations were 0, 0.34, 0.52, 0.83, 1.4 and 3.4 mg/L. At 96 hours, 20 and
100% mortality occurred at 1.2 and 3.4 mg/L, respectively.
96-h LCso = 2.05 mg/L
Acute Toxicity to Aquatic Invertebrates
Gammarids (Gammarus fasciatus) were exposed to 3,4-dichloro-d,d,d-trifluorotoluene at
nominal concentrations of 0, 0.89, 1.4, 2.1, 3.3 or 5 mg/L under flow-through conditions for 96
hours. Measured concentrations were 0, 0.52, 0.83, 1.3, 1.9 and 2.8 mg/L. At 96 hours, 70 and
100% mortality occurred at the two highest concentrations tested.
96-h LCso = 1.7 mg/L
Toxicity to Aquatic Plants
Green algae (Pseudokirchneriella subcapitata) were exposed to 3,4-dichloro-d,d,a-
trifluorotoluene at nominal concentrations of 0, 3.1, 6.3, 13, 25 or 50 mg/L for 96 hours.
Measured concentrations were 0, 0.40, 0.62, 1.8, 3.7 and 8.6 mg/L. Algae growth/cell density
was not inhibited at any of the test concentrations compared to the controls.
96-h LCso > 8.6 mg/L (highest concentration tested)
Chronic Toxicity to Fish
Rainbow trout (O. mykiss) were exposed to 3,4-dichloro-d,d,d-trifluorotoluene at nominal
concentrations of 0, 0.034, 0.068, 0.13, 0.25 or 0.51 mg/L under static conditions for 89 days (60
days post-hatch). Measured concentrations were not specified. Parameters tested included
embryo viability, survival of organism at hatch and survival and growth post-hatch. No effects
on embryo viability or hatchability were noted at any concentration. At the two highest
concentrations, an adverse effect on mean total length was noted. The only reduction in weight
was noted at the highest concentration tested. The maximum acceptable toxicant concentration
was calculated to be 0.18 mg/L.
LOEC = 0.25 mg/L (based on decreased mean total length)
NOEC = 0.13 mg/L
Chronic Toxicity to Invertebrates
Water fleas (Daphnia magna) were exposed to 3,4-dichloro-d,d,d-trifluorotoluene at mean
measured concentrations of 0, 0.03, 0.06, 0.12, 0.24 or 0.38 mg/L for 21 days. Mortality was
markedly increased at all concentrations except 0.03 mg/L. At 0.24 mg/L, the organisms
produced fewer young and those treated at 0.38 mg/L didn't produce any young.
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21-d LCso = 0.16 mg/L
LOEC = 0.06 mg/L (based on mortality)
NOEC = 0.03 mg/L
Conclusion: The 96-h LCso for fish and aquatic invertebrates from 3,4-dichloro-d,d,a-
trifluorotoluene is 2.05-2.3 and 1.7 mg/L, respectively. The 96-h ECso to aquatic plants for
growth rate is > 8.6 mg/L. The 21-d LCso for chronic aquatic invertebrates from 3,4-dichloro-
d,d,d-trifluorotoluene is 0.16 mg/L.
Table 4. Summary of the Screening Information Data Set as Submitted under
the U.S. HPV Challenge Program- Aquatic Toxicity
Endpoints
3,4-Dichloro-d,d,d-benzotrifluoride
(328-84-7)
Fish
96-h LCso (mg/L)
2.05-2.3
Aquatic Invertebrates
96-h LCso (mg/L)
1.7
Aquatic Plants
96-h EgC5o(mg/L)
>8.6
Chronic Aquatic Invertebrates
21-d LCso (mg/L)
0.16
Bold = experimental data (i.e., derived from testing).
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