US Environmental Protection Agency Office of Pesticide Programs Office of Pesticide Programs Microbiology Laboratory Environmental Science Center, Ft. Meade, MD Standard Operating Procedure for Handling Spills of Biohazardous Material SOP Number: MB-13-03 Date Revised: 05-13-11 ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 1 of 15 EPA/OPP MICROBIOLOGY LABORATORY ESC, Ft. Meade, MD Standard Operating Procedure for Handling Spills of Biohazardous Material SOP Number: MB-13-03 Date Revised: 05-13-11 Initiated By: Date: / / Print Name: Technical Review: Date: / / Print Name: Technical Staff QA Review: Date: / / Print Name: QA Officer Approved By: Date: / / Print Name: Branch Chief SHEM Review: Date: / / Print Name: SHEM Manager Effective Date: / / Controlled Copy No.: Withdrawn By: Date: / / ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 2 of 15 TABLE OF CONTENTS Contents Page Number 1.0 SCOPE AND APPLICATION 4 2.0 DEFINITIONS 4 3.0 HEALTH AND SAFETY 5 4.0 CAUTIONS 6 5.0 INTERFERENCES 6 6.0 PERSONNEL QUALIFICATIONS 7 7.0 SPECIAL APPARATUS AND MATERIALS 7 8.0 INSTRUMENT OR METHOD CALIBRATION 8 9.0 SAMPLE HANDLING AND STORAGE 8 10.0 PROCEDURE AM) ANALYSIS 8 10.1. Reporting Instructions 8 10.2 Recommendations for Reducing Potential for Spills 8 10.3. Spills of Biohazardous Organisms Requiring Biosafety Levels 1 and 2 Containment 9 10.4. Spills of Biohazardous Organisms Requiring Biosafety Level 3 Containment (inside BSC) 10 10.5. Spills with Potential for Exposure to Bacillus anthracis (inside BSC) 11 10.6 Spills of Biohazardous Organisms Requiring Biosafety Level 3 Containment (outside BSC) 12 10.7 Decontamination of Clothing 13 ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 3 of 15 TABLE OF CONTENTS (continued) Contents Page Number 11.0 DATA ANALYSIS/CALCULATIONS 14 12.0 DATA MANAGEMENT/RECORDS MANAGEMENT 14 13.0 QUALITY CONTROL 14 14.0 NONCONFORMANCE AND CORRECTIVE ACTION 14 15.0 REFERENCES 15 16.0 FORMS AND DATA SHEETS 15 ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 4 of 15 1.0 SCOPE AND APPLICATION: 1.1 The protocol presents guidelines for decontamination and clean up of biohazardous spills. Periodically, the SOP distinguishes between large spills vs. small spills, and spills inside vs. outside of the biological safety cabinet. Procedures for responding to a spill may vary, depending upon the degree and location of the spill of biohazardous material. 1.2 The OPP Microbiology Laboratory Branch (MLB) holds a select agent registration to work with the select agent Bacillus anthracis in room B202. This SOP also provides guidance on handling spills of B. anthracis. 2.0 DEFINITIONS: 2.1 Mycobacterium bovis (BCG) is a live attenuated vaccine strain used to vaccinate humans against infection by Mycobacterium tuberculosis. 2.2 BSC = biological safety cabinet 2.3 PPE = personal protective equipment 2.4 CDC = Centers for Disease Control and Prevention 2.5 NIH = National Institutes of Health 2.6 "Appropriate" disinfectant = EPA-registered hospital disinfectant (efficacious against S. aureus, P. aeruginosa, and S. choleraesuis), hospital disinfectant with tuberculocidal claims (efficacious against S. aureus, P. aeruginosa, S. choleraesuis, and M. bovis (BCG)), or EPA-registered disinfectant towelette efficacious against spore-former Clostridium difficile. All disinfectants must be used according to the directions (e.g., use dilution, contact time, etc.) specified on the labeling. 2.7 Spill = A spill is defined as a biohazardous material out of control. The quantity of the biohazardous material spilled is not the sole determining factor in deciding whether or not an event is classified as a spill. Rather, the essential issue is whether the biological agent, the location, and the quantity collectively cause the situation to be beyond the control of the laboratory worker. A major spill is one that cannot be handled safely by laboratory employees in the immediate area. A minor spill is one which can be handled by the laboratory workers in the immediate area without posing a serious threat to their health and safety, and that can be cleaned up with available absorbents and disinfectants. ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 5 of 15 2.8 OEP = Occupant Emergency Plan 2.9 CHP = Chemical Hygiene Plan 2.10 Restrooms = Restrooms equipped with showers (e.g., D222 and D223) 2.11 References to water mean reagent-grade water 2.12 P100 HEPA = Oil proof High-Efficiency Particulate Air filter, 99.97% efficient in removing particles 0.3 microns or larger HEALTH AND SAFETY: 3.1 To protect the laboratory worker from possible infection by microorganisms, the health and safety guidelines provided in SOP MB-01 (Biosafety in the Laboratory) and in the Centers for Disease Control and Prevention/National Institutes of Health (CDC/NIH) "Biosafety in Microbiological and Biomedical Laboratories," (BMBL) 5th ed. (see ref. 15.1) manual must be followed. All laboratory personnel are required to read and familiarize themselves with SOP MB-01 and sections on Biosafety Levels 2 and 3 in the BMBL. 3.2 Spills and accidents are handled according to the practices outlined in this SOP, as well as procedures referenced in the ESC OEP and CHP. See section 10.1 for instructions for reporting spills to the Branch Chief and SHEM (Safety, Health, and Environmental Management) manager. Exercises or drills to practice managing spills according to the procedures in this SOP will be conducted annually. 3.3 Procedures for handling spills of biosafety level 3 microorganisms, including Bacillus anthracis, are divided into two categories: 1) spills occurring inside of the BSC, and 2) spills occurring outside of the BSC. Serious or lethal disease may result from inhalation of a biosafety level 3 microorganism. If the BSC remains operational following a spill, infectious aerosols resulting from a spill inside of the BSC are contained, and the analysts do not need to evacuate the laboratory to avoid exposure. Infectious aerosols resulting from spills outside of the BSC are not contained; hence the need for different responses, depending upon the location of the spill. In the event of a spill of a biosafety level 3 microorganism outside of the BSC, workers are to follow procedures in Table 4 . ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 6 of 15 3.4 In the event that analysts are unable to reach the SHEM manager, they are to call security at extension 2800 and report the spill. Security will locate the SHEM manager or his designee. 4.0 CAUTIONS: 4.1 Lack of use or understanding of this SOP may negatively impact the decontamination efforts of laboratory staff and hence, cause unnecessary exposure of employees to biohazardous microorganisms. 4.2 Failure to clean the ultraviolet lamps in the BSCs will reduce the lamps' effectiveness. Periodically clean the ultraviolet lamps in the BSCs with a lint-free cloth dampened with alcohol. 4.3 Do not use a liquid bleach solution to decontaminate stainless steel surfaces (e.g., BSC) following a spill of biohazardous material, including spore-forming bacteria (e.g., Bacillus subtilis), unless the microorganism is a select agent (i.e., Bacillus anthracis). In the event that a spill occurs in the BSC involving Bacillus anthracis, treat the stainless steel surfaces with a liquid bleach solution as described in section 7.6.2. Following treatment, the surface must be rinsed with water, 70% ethanol, or an EPA-registered liquid disinfectant to remove excess sodium hypochlorite. 4.4 Recently, the Agency has registered sodium hypochlorite towelettes which claim effectiveness against the spore-forming microorganism, Clostridium difficile. In the event that a spill of C. difficile occurs inside the BSC (i.e., steel surface), these towelette products may be used to disinfect the surface. However, following the duration of the towelette contact time, the surface must be rinsed with water, 70% ethanol, or an EPA-registered liquid disinfectant to remove excess sodium hypochlorite. 5.0 INTERFERENCES: 5.1 Failure to become familiar with and to put into practice the procedures set forth in this SOP will result in analysts who are a danger to themselves, others, and the environment. 5.2 Improper maintenance and/or expired certification may result in failure of the BSCs to operate properly. Refer to proper use and maintenance procedures in SOP QC-06, Use and Maintenance of Biological Safety Cabinets. ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 7 of 15 6.0 PERSONNEL QUALIFICATIONS: 6.1 Personnel are required to be knowledgeable of the procedures in this SOP. Documentation of training and familiarization with this SOP can be found in the training file for each employee. 7.0 SPECIAL APPARATUS AND MATERIALS: 7.1 Autoclave 7.2 Biohazard bags (clear in color, autoclavable) or containers inside and outside of the biological safety cabinets for collection and storage of biohazardous waste. 7.3 When specified, personal protective equipment (PPE) such as gloves, safety glasses, lab coats, disposable laboratory garments, shoe covers, respiratory protection (respirators with PI 00 HEP A filter cartridges), and temporary clothing (i.e., scrubs). 7.4 Biosafety Spill Kit containing items such as autoclave bag, latex gloves, gloves for handling broken glass, dustpan/brush, shoe covers, disposable lab coat, paper towels, disinfectant (with hospital disinfectant and tuberculocidal efficacy), and safety glasses. 7.5 Appropriate signs to identify biohazardous materials and to limit access to laboratories. 7.6 Bleach solutions made fresh as needed. Discard solution at the end of the day. When a new container of bleach is opened, the analyst will note the date opened on the container. The container of bleach will be discarded six months from the date of receipt. 7.6.1 1:10 diluted bleach solution for decontamination purposes (vegetative cells). Using an EPA-registered sodium hypochlorite product, dilute as follows: 1 part bleach, 9 parts water. 7.6.2 1:10 diluted bleach solution at neutral pH for decontamination purposes (spore-forming microorganisms). Using an EPA-registered sodium hypochlorite product containing approximately 6% sodium hypochlorite, dilute as follows: 1 part bleach, 8.4 parts water, and 0.6 parts 5% white vinegar or 5% lab grade acetic acid. ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 8 of 15 7.7 Appropriate disinfectants for microorganisms specified in Attachment A of SOP MB-01, Biosafety in the Laboratory. 7.7.1 For microorganisms in vegetative form, use a 1:10 diluted bleach solution as described in section 7.6.1 or EPA- registered hospital di sinfectant/tub ercul oci de. 7.7.2 For spore-forming microorganisms, prepare the adjusted bleach solution described in section 7.6.2. 7.8 Key card readers are used to limit access to testing laboratories. Only authorized personnel are permitted to enter. 8.0 INSTRUMENT OR METHOD CALIBRATION: Not applicable 9.0 SAMPLE HANDLING AND STORAGE: Not applicable 10.0 PROCEDURE AND ANALYSIS: 10.1 Guidance for Spills of Biohazardous Material - Reporting Instructions 10.1.1 Accidents are handled according to the practices outlined in this subpart, as well as procedures referenced in the OEP and the ESC CHP. 10.1.2 All spills and accidents involving a Biosafety Level 2 or 3 microorganism, regardless of how minor a spill, are reported to the Branch Chief and the SHEM manager (or call security desk at extension 2800). 10.1.3 The Branch Chief is responsible for documenting spills and accidents. 10.1.4 The Branch Chief and SHEM manager will determine if additional written documentation or follow-up is warranted. 10.2 Recommendations for Reducing Potential for Spills of Biohazardous Material 10.2.1 Use secondary containment (e.g., autoclave bin) when transporting live cultures in liquid media or on solid media. ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 9 of 15 10.2.2 Use secondary containment to store biohazardous waste (plates and tubes) that is generated during the course of an assay. 10.2.3 Prepare the least amount of culture necessary for an assay. 10.2.4 Maintain a clean, well-organized work environment. 10.3 Guidance for Spills of Biohazardous Organisms Requiring Biosafety Level 1 and 2 Containment, (see Attachment A) - Spills Outside and Inside the BSC. See the summary table (Table 1) below: Table 1: liiosalelv Level 1 and 2 Spill Control Procedures Spills Outside Inside of tlic HSC Notify Workers/SI IKM Manager/I .aboniton I )irector (or Security) Alert workers in the laboratory that a spill has occurred. Contact the SI IKM Manager (or call security desk at extension 2800) and Branch Chief. Maintain I JSC air flow Keen the BSC operational. Do not turn oil" the blower for am reason. 'frail I Iuman Injun Treat injury or call 911. depending upon the severity of the injury. Control the Source Stand the spilled bottle upright, and unplug any small contaminated equipment (i.e.. vortex, timer). Control the Spread Cover the spill with a paper towel (not plastic-backed material) or other appropriate absorbent material (e.g.. Isosorb 13000) to prevent the spill area from growing in si/e. Remove Contaminated Clothing Remove any contaminated gloves, lab coal, and street clothing and place in an auloclavable container or bag. Wash contaminated skin as described below. Replace any contaminated street clothing with temporary clothing such as scrubs (located in B202 and B207). or jumpsuit from the shower area of the restrooms 1)222 and 1)223. Wash Contaminated Skin for small spills on the skin, wash skin thoroughly with soap and water and don temporary clothing, for large spills to a significant portion of the body and clothing, disrobe, put on temporary clothing and shoe covers (to protect against shedding of infectious microoganisms in the corridor), wash hands with soap and water, exit the laboratory and proceed to the restroom (1)222 or 1)223) to fully shower for 10 minutes. Discard temporary clothing and shoe covers in auloclavable container or bag. 1 )on new set of temporary clothing (e.g., jumpsuit in restroom). 1 )econtaminate Area Vegetative cells: Saturate the paper towel or other absorbent material, starting with the edges of the spill and working towards the center, with liquid disinfectant (e.g.. 1:10 bleach solution, section 7.6.11 fPA registered hospital disinfectant, section 7.7.1) and let stand for 20 to 30 minutes. Do not use a bleach solution on stainless steel surfaces of the BSC. Spore-forming bacteria: for spills of spore-forming bacteria on non-stainless steel surfaces outside of the BSC. use a 1:10 diluted bleach solution at neutral pi 1 ( section 7.6.2) for a 60- minute contact time, for spills off'. difficile in the BSC (stainless steel), wipe surface with a sodium hypochlorite-containing towelette registered by the Agency for effectiveness against the microorganism. After the duration of the label-specified contact time, wash the surface with water. 70% ethanol. or an Kl'A-resiislered disinfectant to remove excess ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 10 of 15 sodium hypochlorite, l-'or spills of other BSL-I and -2 spore-lbrming bacteria in the BSC (stainless steel), wipe or spray surface with a non-bleach liquid disinfectant and wipe up spill with paper towels to physically remove spores. Turn on IJV light overnight. Clam Up Pick up absorbent material and place in autoclavable container or bag. Use thick gloves, scoop, and brush found in biosafety spill kit to clean up broken glass. Place in autoclavable container. Inspect the Area Carefully check the entire affected area and beyond for remaining spill or cleanup residue. Decontaminate all clean-up material and PPl! Autoclave contaminated paper towel or other absorbent material and any contaminated broken glass and writing utensils according to SOP MB-OI. Biosafety in the Laboratory. Treat contaminated gloves (for handling broken glass), scoop, and brush with disinfectant or place under the LJV light overnight. Treat contaminated PPL! and any contaminated street clothing w ith disinfectant or autoclave as described in section 10.7. 1 )iscard autoclaved broken glass in a broken glass container. 10.4 Guidance for Spills of Biohazardous Organisms Requiring Biosafety Level 3 Containment (Excluding Bacillus anthracis) - Spills contained within the BSC (bioaerosols will be contained by the BSC's laminar air flow). See the summary table (Table 2) below: Table 2: Biosafety Level 3 Spill Control Procedures (Excluding Bacillus anthracis) Spills Inside of the BSC Notify Workers/SHEM Manager/Laboratory Director Alert workers in the laboratory that a spill has occurred. Contact the STEM Manager (or call security desk at extension 2800) and the Branch Chief. Maintain BSC Air Flow Keen the BSC operational. Do not turn off the blower for anv reason. Treat Human Injury Treat injury or call 911, depending upon the severity of the injury. Control the Source Stand the spilled bottle upright, and unplug any small contaminated equipment (i.e., vortex, timer). Control the Spread Cover the spill with a paper towel (not plastic-backed material) or other appropriate absorbent material (e.g., Isosorb 13000) to prevent the spill area from growing in size. Remove Contaminated Clothing Remove contaminated gloves, lab coat, and street clothing and place in an autoclavable container or bag. Wash contaminated skin as described below. Replace contaminated street clothing with temporary clothing such as scrubs (located in B202 and B207 double- door access rooms). Wash Contaminated Skin For small spills on the skin of the hands or forearms, wash skin thoroughly with soap and water. Decontaminate Area Saturate the paper towel or other absorbent material, starting with the edges of the spill and working towards the center, with liquid disinfectant (e.g., EPA registered hospital disinfectant, section 7.7.1) and let stand for 20 to 30 minutes. Do not use a bleach solution on stainless steel surfaces of the BSC. ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 11 of 15 Clean Up Pick lip absorbent material and place in autoclavable container or bag. Use thick gloves, scoop, and brush found in biosaletv spill kit to clean up broken glass. Place glass in autoclavable container. Inspect the Area Carefully check the entire affected area and beyond for remaining spill or cleanup residue. Decontaminate all clean-up material and PPl ! Autoclave contaminated paper towel or other absorbent material and any contaminated broken glass and writing utensils according to SOP MB-OI. Biosalety in the Laboratory. Treat contaminated gloves (for handling broken glass), scoop, and brush with disinfectant or place under the U V light overnight, 'frail contaminated PPland any contaminated street clothing w ilh disinfectant or autoclave as described in section 10.7. 1 )iscard auloclaved broken glass in a broken glass container. 10.5 Guidance for Spills of Materials with Potential Risk of Exposure to Bacillus anthracis - Spills contained within the BSC (bioaerosols will be contained by the BSC's laminar air flow). See the summary table (Table 3) below: Table 3: Spill Control Procedures for Bacillus anthracis Spills Inside of the BSC' Notify Workers Alert workers in the laboratory that a spill has occurred in the BSC. Notify the SI ILM Manager and 1 Jranch Chief. Maintain BSC Air flow Keep the BSC operational. Do not turn offlhe blower for am reason. Keep 1 lands Inside the BSC Analyst must keep his or her hands inside olThe BSC until the spill is cleaned up and the outer layer of gloves has been removed. ()lher analysis (unconlaminaled) in the laboratory will assist by supplying the necessary cleanup materials. Spill iiiilii .\l>M>rl>i-nl Pudding If the spill occurred onto absorbent padding, return the overturned llask. tube. etc.. to an upright position. Place the contaminated absorbent padding and other items into the autoclavable waste container in the BSC. Wipe down the BSC with 70% ethanol or LPA registered hospital disinfectant (section 7.7.1). replace the absorbent padding and resume work. Upon completion of work in the BSC, turn on the UV light for a minimum of 15 hours to decontaminate the area olThe spill. Spill diiviily mi liSC Surhui- (m> ;il)surl)i-|ll p;i(l(lill<;) If the spill occurred on the surface olThe BSC, proceed through the steps below. Control the Spread Return overturned llask, tube. etc. to an upright position. Cover the spill with paper towels (not plastic-backed paper) or other absorbent material to prevent the spill area from growing in si/e. Unplug any small contaminated equipment (i.e.. vortex, timer). Pre-clean/Physical Removal of Spores Saturate paper towel or other absorbent material, starling with the edges and moving towards the center, with a 1:10 diluted bleach solution at neutral pi 1 (section 7.6.2). Let stand for a minimum often minutes. Clean Up Use thick gloves, scoop, and brush found in biosaletv spill kit to clean up broken glass. Place glass in autoclavable container inside the BSC. Wipe up liquid with paper towels, flush the BSC with water. 70% ethanol. or LPA registered hospital disinfectant (section 7.7.1) to remove excess sodium hypochlorite. Wipe up the liquid with paper towels. Place all absorbent material/paper towels in the autoclave bin inside the BSC. ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 12 of 15 Inspect the Area Carefully check the entire affected area and beyond for remaining spill or cleanup residue. Turn on UV Light in BSC Once the spill has been pre-cleaned and the liquid wiped up, turn on the UV light for a minimum of 15 hours. Decontaminate all clean-up material and PPE Autoclave biohazardous waste, including gloves/brush/scoop used to pick up broken glass, using a 3 hour liquid kill cycle for liquid and/or solid waste at 121 °C. Discard autoclaved broken glass in a broken glass container. 10.6 Guidance for Spills of Biohazardous Organisms Requiring Biosafety Level 3 Containment - Spills Outside of the BSC. See the summary table (Table 4) below: Table 4: Biosafety Level 3 Spill Control Procedures Spills Outside of the BSC Notify Workers/SHEM Manager/Laboratory Director (or Security) Alert workers in the laboratory that a spill has occurred. Contact SHEM Manager (or call security desk at extension 2800) and Branch Chief. If wearing a respirator when spill occurs, workers should remove PPE as described below*, exit laboratory to hallway, and alert SHEM Manager and Branch Chief. For human injury, treat injury, call 911 and/or security at extension 2800, depending upon the severity of the issue. Maintain BSC Air Flow Keen the BSC operational. Do not turn off the blower for anv reason. Pre-evacuation Procedures Stop all work and close all open materials. Ensure microorganism-containing materials are secured with appropriate secondary containment in the BSC. Close sash. (If select agent is in use, place the secondary containment bin in a secure location such as a refrigerator or incubator.) Close any open biohazardous bags outside the BSC. *Evacuate Laboratory - non-select agent microorganism Remove labcoat, gloves, and shoe covers, discarding them in the laboratory biohazard bin. Exit the laboratory to the anteroom. *Evacuate Laboratory - Bacillus anthracis- specific Remove labcoat, shoe covers, and outer layer of gloves and place in laboratory biohazard bin. Keep on respirator and inner layer of gloves. Exit laboratory to anteoom. Remove respirator and then inner gloves. Place in bin in anteroom. Remove Contaminated Clothing Once in the double-door access room, remove contaminated street clothing and place in an autoclavable container or bag. Wash contaminated skin as described below. Replace contaminated street clothing with temporary clothing such as scrubs (located in B202 and B207 double-door access rooms). Wash hands in the sink in the anteroom. Wash Contaminated Skin For minor spills on the skin, wash skin thoroughly with soap and water. For major spills to a significant portion of the body and clothing, disrobe in the anteroom put on temporary clothing, gloves, and shoe covers, exit the laboratory and proceed to the restroom (D222 or D223) to fully shower for 10 minutes. Discard ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 13 of 15 temporary clothing, gloves, and shoe covers in an autoclavable container or bag. Don new set of temporary clothing (e.g., jumpsuit in restroom). Direct Workers to Slav Out Direct (via telephone, note 011 door, etc.) analysis not present in the laboratory to slay out until spill clean up is complete. Allow Facility'"s Ventilation to Remove Aerosols Wait at least 30 minutes before re-entering the laboratory to allow the FSC's exhaust air ventilation system to remove bioaerosols. Re-Fliter the Laboratory (SI IFM/Responders) Re-enter the laboratory with appropriate level of PPF as determined by SI IFM and internal/external responders. Survey the spill site for location and amount of spill. Cover the Spill Area Cover the spill w ith a paper low el (not plastic-backed material) or other appropriate absorbent material (e.g.. Isosorb 13000) as appropriate to site and location of spill. Unplug any small contaminated equipment (i.e.. vortex, tinier). 1 )econtaminate Area Saturate the paper towel or other absorbent material, starting w ith the edges of the spill and working towards the center, with liquid disinfectant (e.g., 1:10 bleach solution, section 7.6.111IPA registered hospital disinfectant, section 7.7.1) and let stand for 20 to 30 minutes. Do not use bleach solution 011 stainless steel surfaces. For spills 0['Bacillus cinlliracis (outside of BSC/11011-slainless steel surface): Use a 1:10 diluted bleach solution at neutral pi 1 ( section 7.6.2) for a 60-minute contact time. Analysis may exit the laboratory to await the completion of disinfectant contact time, but must follow the approporiate PPF donning and dolling procedures as described above*. Clean Up Wearing appropriate PPF. pick up absorbent material and place in autoclavable container or bag. Use thick gloves, scoop, and brush found in biosafety spill kit to clean up broken glass. Place glass in autoclavable container. Wash (with water. 70% ethanol. or FPA registered hospital disinfectant) and dry all areas where sodium hypochlorite was applied. Inspect the Area Carefully check the entire affected area and beyond for remaining spill or cleanup residue. Decontaminate all clean-up material and PPF Autoclave contaminated paper towel or other absorbent material and any contaminated broken glass and writing utensils according to SOP Ml J-01, Biosafety in the Laboratory. For 11011-selecl agent spills, treat contaminated gloves (for handling broken glass), scoop, and brush w ith disinfectant or place under the U V light overnight. For select agent spills, autoclave and dispose of all clean up materials. Treat contaminated PPL and any contaminated street clothing w ith disinfectant or autoclave as described in section 10.7. 1 )iscard autoclaved broken glass in a broken glass container. Review and Reassess Following completion of all clean-up activities, review procedures to determine if changes are necessary. Replace items in the spill kit. 10.7 Decontamination of Cloth Lab Coats, Street Clothing, and Footwear 10.7.1 Decontaminate clothing with an appropriate disinfectant or by autoclaving. ------- SOP No. MB-13-03 Date Revised 05-13-11 Page 14 of 15 10.7.2 If using disinfection as a means of decontamination, treat area of contamination and surrounding area with disinfectant for label- specified dilution and contact time (section 7.7.1). 10.7.3 Clothing potentially contaminated with microorganisms in spore form must be autoclaved according to MB-01. 10.7.4 It is less harmful to clothing to autoclave it in a tray than it is to bag it. Do not put water in the tray with the lab coat. Rather, put a second tray into the autoclave and add water to this tray. 10.7.5 After clothing is decontaminated (by disinfection or autoclaving), immerse it in water containing detergent to aid physical removal of decontaminated biohazardous material. 10.7.6 Rinse lab coat and set aside to be sent out with the weekly lab coat laundry service. 10.7.7 Take street clothing and footwear home and launder. 11.0 DATA ANALYSIS/CALCULATIONS: None 12.0 DATA MANAGEMENT/RECORDS MANAGEMENT: 12.1 The Branch Chief is responsible for documenting spills and accidents. 13.0 QUALITY CONTROL: 13.1 Appropriate quality control measures are integrated into each SOP. 14.0 NONCONFORMANCE AND CORRECTIVE ACTION: 14.1 Strict adherence to the biosafety practices is required. Nonconformance will result in notification, retraining, or disciplinary action of laboratory employees. 15.0 REFERENCES: 15.1 Centers for Disease Control and Prevention and National Institutes of Health, 2007. Biosafety in Microbiological and Biomedical Laboratories, 5th edition. U.S. Department of Health and Human Services. U.S. Government Printing Office, Washington, D.C. ------- 16.0 FORMS AND DATA SHEETS: None SOP No. MB-13-03 Date Revised 05-13-11 Page 15 of 15 ------- |