Screening-level Hazard Characterization of High Production Volume Chemicals Sponsored Chemical Methane Sulfonic Acid (cas No. 75-75-2) [9th Ci Name Methane Sulfonic Acid] August 2007


SCREENING-LEVEL HAZARD CHARACTERIZATION
OF HIGH PRODUCTION VOLUME CHEMICALS

SPONSORED CHEMICAL

Methane Sulfonic Acid (CAS No. 75-75-2)
[9th CI Name: Methane Sulfonic Acid]

August 2007

Prepared by

High Production Volume Chemicals Branch
Risk Assessment Division
Office of Pollution Prevention and Toxics
Environmental Protection Agency
1200 Pennsylvania Avenue, NW
Washington, DC 20460-0001

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SCREENING-LEVEL HAZARD CHARACTERIZATION
OF HIGH PRODUCTION VOLUME CHEMICALS

The High Production Volume (HPV) Challenge Program1 is a voluntary initiative aimed at developing and making
publicly available screening-level health and environmental effects information on chemicals manufactured in or
imported into the United States in quantities greater than one million pounds per year. In the Challenge Program,
producers and importers of HPV chemicals voluntarily sponsor chemicals; sponsorship entails the identification and
initial assessment of the adequacy of existing toxicity data/information, conducting new testing if adequate data do
not exist, and making both new and existing data and information available to the public. Each complete data
submission contains data on 18 internationally agreed to "SIDS" (Screening Information Data Set1'2) endpoints that
are screening-level indicators of potential hazards (toxicity) for humans or the environment.

The Environmental Protection Agency's Office of Pollution Prevention and Toxics (OPPT) is evaluating the data
submitted in the HPV Challenge Program on approximately 1400 sponsored chemicals. OPPT is using a hazard-
based screening process to prioritize review of the submissions. The hazard-based screening process consists of two
tiers described below briefly and in more detail on the Hazard Characterization website3.

Tier 1 is a computerized sorting process whereby key elements of a submitted data set are compared to established
criteria to "bin" chemicals/categories for OPPT review. This is an automated process performed on the data as
submitted by the sponsor. It does not include evaluation of the quality or completeness of the data.

In Tier 2, a screening-level hazard characterization is developed by EPA that consists of an objective evaluation of
the quality and completeness of the data set provided in the Challenge Program submissions. The evaluation is
performed according to established EPA guidance2'4 and is based primarily on hazard data provided by sponsors.
EPA may also include additional or updated hazard information of which EPA, sponsors or other parties have
become aware. The hazard characterization may also identify data gaps that will become the basis for a subsequent
data needs assessment where deemed necessary. Under the HPV Challenge Program, chemicals that have similar
chemical structures, properties and biological activities may be grouped together and their data shared across the
resulting category. This approach often significantly reduces the need for conducting tests for all endpoints for all
category members. As part of Tier 2, evaluation of chemical category rationale and composition and data
extrapolation(s) among category members is performed in accord with established EPA2 and OECD5 guidance.

The screening-level hazard characterizations that emerge from Tier 2 are important contributors to OPPT's existing
chemicals review process. These hazard characterizations are technical documents intended to support subsequent
decisions and actions by OPPT. Accordingly, the documents are not written with the goal of informing the general
public. However, they do provide a vehicle for public access to a concise assessment of the raw technical data on
HPV chemicals and provide information previously not readily available to the public. The public, including
sponsors, may offer comments on the hazard characterization documents.

The screening-level hazard characterizations, as the name indicates, do not evaluate the potential risks of a chemical
or a chemical category, but will serve as a starting point for such reviews. In 2007, EPA received data on uses of
and exposures to high-volume TSCA existing chemicals, submitted in accordance with the requirements of the
Inventory Update Reporting (IUR) rule. For the chemicals in the HPV Challenge Program, EPA will review the
IUR data to evaluate exposure potential. The resulting exposure information will then be combined with the
screening-level hazard characterizations to develop screening-level risk characterizations4'6. The screening-level
risk characterizations will inform EPA on the need for further work on individual chemicals or categories. Efforts
are currently underway to consider how best to utilize these screening-level risk characterizations as part of a risk-
based decision-making process on HPV chemicals which applies the results of the successful U.S. High Production
Volume Challenge Program and the IUR to support judgments concerning the need, if any, for further action.

1 U.S. EPA. High Production Volume (HPV) Challenge Program; http://www.epa.gov/chemrtk/index.htm.

2 U.S. EPA. HPV Challenge Program - Information Sources; http://www.epa.gov/chemrtk/pubs/general/guidocs.htm.

3 U.S. EPA. HPV Chemicals Hazard Characterization website (http://www.epa.gov/hpvis/abouthc.html).

4 U.S. EPA. Risk Assessment Guidelines; http://cfpub.epa.gov/ncea/raf/rafguid.cfm.

5 OECD. Guidance on the Development and Use of Chemical Categories; http://www.oecd.org/dataoecd/60/47/1947509.pdf.

6 U.S. EPA. Risk Characterization Program; http://www.epa.gov/osa/spc/2riskchr.htm.

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SCREENING-LEVEL HAZARD CHARACTERIZATION
Methane Sulfonic Acid (CAS No. 75-75-2)

Introduction

The sponsor, Atofina Chemicals, Inc and Chevron Phillips Chemical Company LP, submitted a Test Plan and
Robust Summaries to EPA for methane sulfonic acid (CAS No. 75-75-2; 9th CI name: methane sulfonic acid) dated
January 10, 2003. EPA posted the submission on the ChemRTK HPV Challenge Web site on January 17, 2003
(http://www.epa.gov/chemrtk/pubs/summaries/methanes/cl4249tc.htm'). EPA comments were submitted on June
17, 2003. Public comments were also received and posted to the website. The sponsor responded on August 25,
2003.

This screening-level hazard characterization is based primarily on the review of the test plan and robust summaries
of studies submitted by the sponsor(s) under the HPV Challenge Program. In preparing the hazard characterization,
EPA considered its own comments and public comments on the original submission as well as the sponsor's
responses to comments and revisions made to the submission. A summary table of SIDS endpoint data with the
structure(s) of the sponsored chemical(s) is included in the appendix. The screening-level hazard characterization
for environmental and human health toxicity is based largely on SIDS endpoints and is described according to
established EPA or OECD effect level definitions and hazard assessment practices.

Summary-Conclusion

The log Kow of methane sulfonic acid indicates its potential to bioaccumulate is expected to be low. Methane
sulfonic acid is not readily biodegradable, indicating it has the potential to persist in the environment.

The evaluation of available aquatic toxicity data for fish, aquatic invertebrates and aquatic plants indicates the
potential acute hazard of methane sulfonic acid to aquatic organisms is moderate.

Methane sulfonic acid showed moderate acute oral toxicity to rats and moderate acute dermal toxicity to rabbits.
Methane sulfonic acid is corrosive to mouse skin, extremely corrosive to the eye and none of the test animals
responded with a skin grade that would have been suggestive of sensitization. Following repeated nose-only aerosol
exposure of rats for 4 weeks, exposure-related clinical signs consisted of rales and an increased incidence of yellow
matting on various body surfaces. A transient reduction in mean body weight gain of high-exposed males during the
first week of exposure was observed. Test substance-related microscopic findings were observed in the nasal
turbinates of the high-exposure group rats that were found dead and in all treated groups at study week 4 and after 2
weeks recovery period. No effects were seen for parental toxicity or reproductive performance in a combined
rcproductivc/loxicily screening test. Available developmental toxicity data showed maternal toxicity but no
developmental effects were evident. Methane sulfonic acid was not mutagenic and did not induce chromosomal
aberrations.

The potential health hazard of methane sulfonic acid is moderate based on repeated-dose toxicity and maternal
toxicity observed in the developmental toxicity tests.

No data gaps were identified under the HPV Challenge Program.

1. Physical-Chemical Properties and Environmental Fate

A summary of physical-chemical properties and environmental fate data submitted is provided in the Appendix. For
the purpose of the screening-level hazard characterization, the review and summary of these data was limited to the
octanol-water partition coefficient and biodegradation endpoints as indictors of bioaccumulation and persistence,
respectively.

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Octanol-water Partition Coefficient

Log Kow: -4.98

Biodegradation

In a closed-bottle test, 100% degradation of methane sulfonic acid was observed after 28 days.

Methane sulfonic acid is readily biodegradable.

Conclusion: The log Kow of methane sulfonic acid indicates its potential to bioaccumulate is expected to be low.
Methane sulfonic acid is readily biodegradable, indicating it does not have the potential to persist in the
environment.

2. Environmental Effects - Aquatic Toxicity
Acute Toxicity to Fish

Rainbow trout (Oncorhynchus mykiss; 20/concentration) were exposed to methane sulfonic acid at nominal
concentrations of 0, 13, 22, 37, 60 and 100 mg active ingredient (A.I)/L for 96 hours under static conditions.
Mortality (100%) was seen only at 100 mg A.I./L. To evaluate the effect of pH on the toxicity of the test substance
to the exposed organisms, a duplicate set of exposure solutions at the 60 and 100 mg/L concentrations were prepared
and the pH of these solutions was adjusted to 7.1-7.3 with sodium hydroxide. No mortality occurred with the pH-
adjusted solutions.

96-h LCS0 = 73 mg A.I./L

Acute Toxicity to Aquatic Invertebrates

(1) Daphnia magna (10/concentration) were exposed to methane sulfonic acid at nominal concentrations of 0, 120,
210, 330, 570 or 890 mg A.I./L for 48 hours under static conditions. To evaluate the effect of pH on the toxicity of
the test substance to the exposed organisms, a duplicate set of exposure solutions at the 60 and 100 mg/L
concentrations were prepared and the pH of these solutions was adjusted to 7.9 - 8.3 with sodium hydroxide.
Immobilization of organisms in the pH-adjusted solutions was similar to that of the control, suggesting that the
acidic pH contributed to immobilization in the main test.

48-h ECS0 = 260 mg A.I./L

(2) Daphnia magna (group size not indicated) were exposed to methane sulfonic acid for 24 hours. Test
concentrations, group size and information on adjustment of pH were not provided, but the pH was 3.51. Neither
nominal nor measured concentrations were provided in the robust summary.

24-h ECS0 = 1.7 mg/L

Toxicity to Aquatic Plants

Green algae (Pseudokirchneriella subcapitata) were exposed to methane sulfonic acid at nominal concentrations of
0, 3.1, 6.3, 13, 25, 50 or 100 mg A.I./L with 50 and 100 mg A.I./L adjusted to pH 7.5. Measured concentrations
were 0, 2.7, 5.8, 12, 24, 47 or 96 mg A.I./L with 49 and 95 mg A.I./L adjusted to pH 7.5. In the main test, inhibition
of cell density, biomass and growth rate was 100% at >= 24 mg A.I./L. With adjustment to pH 7.5 cell densities at
49 and 95 mg A.I./L were similar to that of control, suggesting that the acidic pH contributed to inhibition in the
main test.

72-h EC50 (biomass) = 14 mg A.I./L
72-h EC50 (growth rate = 16 mg A.I./L

Conclusion: The evaluation of available aquatic toxicity data for freshwater fish, aquatic invertebrates and aquatic
plants indicates that the potential hazard of methane sulfonic acid to aquatic organisms is moderate.

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3. Human Health Effects

Acute Oral Toxicity

(1) Male and female rats (5/sex) were administered methane sulfonic acid via oral gavage at 300, 500 or 750 mg/kg-
bw and were observed for 14 days. Most clinical abnormalities were observed in both the animals that died and
those that survived to day 14 and included salivation, decreased activity, wobbly gait, breathing abnormalities,
apparent hypothermia, decreased/no defecation, feces small in size, urine stain, hunched posture, unkempt
appearance, rough haircoat, piloerection, extremities pale in color, dehydration, emaciation, distended abdomen,
decreased food consumption and dark material around the facial area. Body weight loss was noted for one 300
mg/kg-bw male, one 500 mg/kg-bw male, two 500 mg/kg-bw females and one 750 mg/kg-bw male during the study
day 0-7 body weight interval. Body weight loss was noted for one 300 mg/kg-bw male and one 750 mg/kg-bw male
during the study day 7-14 body weight interval. Gross internal findings observed in the animals that died included
distension/abnormal content/reddened mucosa in the digestive tract, dark red/mottled lungs, blackish-purple spleens,
dark red lymph nodes, stained glandular mucosa in the stomach and body fat depletion/discoloration/adhesions in
the abdominal cavity.

LDS0 (male) > 750 mg/kg-bw
LDS0 (female) = 461 mg/kg-bw
LDS0 (combined) = 649 mg/kg-bw

(2) Male and female rats (5/sex/dose) were administered methane sulfonic acid via oral gavage at 500, 1000 or 1500
mg/kg-bw and were observed for 14 days. Clinical abnormalities observed during the study included salivation,
breathing abnormalities, wobbly gait, decreased activity, decreased defecation, rough haircoat, urine/fecal stain and
dark material around the facial area. Body weight loss was noted for one 1000 mg/kg-bw male and one 1000
mg/kg-bw female during the study day 0-7 body weight interval and for one 300 mg/kg-bw male and one 1000
mg/kg-bw male during the study day 7-14 body weight interval. In animals that died, gross pathologic findings
included dark red foci on the liver, blackish-purple spleens and abnormal content, reddened/thickened mucosa and
discoloration in the digestive tract.

LDS0 (male) = 860.1 mg/kg-bw
LDS0 (female) = 2407.6 mg/kg-bw
LDS0 (combined) = 1157.5 mg/kg-bw

(3) Male rats (5 rats/dose; except 3 rats in lowest dose) were given administered methane sulfonic acid via oral
gavage at 0.25, 0.5 or 1.0 mL/kg-bw and were observed for 5 days. All rats died by day 5. Sluggishness and
unsteady gait were seen at all doses. At 1.0 mL/kg, deep breathing was immediately observed. Findings at necropsy
included: livers mottled and burned, stomach burned, pylorus hemorrhaged and gas filled, intestines hemorrhaged
and gas filled, kidneys mottled and slightly congested.

LDS0 = 0.281 mL/kg-bw

Acute Dermal Toxicity

(1) New Zealand White rabbits (10) were treated with 1000 mg/kg-bw of methane sulfonic acid for 24 hours and
observed for 14 days.

LDS0 > 1000 mg/kg-bw

(2) Six albino rabbits were treated with 200 or 2000 mg/kg-bw of methane sulfonic acid for 24 hours. At the end of
the exposure period, no systemic toxicity was observed. Two rabbits at 2000 mg/kg-bw died 28 hours after contact
and an additional rabbit was euthanized 3 days after treatment for humane reasons. Severe dermal lesions were
observed.

LDS0 = between 200 and 2000 mg/kg-bw
Repeated-Dose Toxicity

Male and female rats (15/sex/dose) were exposed to methane sulfonic acid aerosol at concentrations of 0, 0.026,
0.073 or 0.242 mg/L for 6 hours/day, 5 days/week for 4 weeks. A 2-week recovery period followed exposure. A
transient reduced mean body weight gain and slightly reduced food consumption was observed in 0.242 mg/L

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exposure level males. Exposure-related clinical signs included rales at 0.242 mg/L and increased incidence of
yellow matting on body surfaces at 0.242 mg/L in both sexes and at 0.073 mg/L in males. Deaths occurred in 4, 1, 1
and 5 animals at 0, 0.026, 0.073 and 0.242 mg/L, respectively, but were attributed to confinement in the restraint
tubes, rather than exposure to test substances. Histopathology lesions in the nasal turbinates were observed in all
exposure groups. During the two-week recovery (non-exposure) period the clinical signs and body weight gains
were completely reversed; histopathology lesions in the nasal turbinates were observed in all groups at the end of the
recovery period.

28-d LOAEL (systemic toxicity) = 0.073 mg/L (based on clinical signs)

28-d NOAEL (systemic toxicity) = 0.026 mg/L

28-d LOAEL (local irritation) = 0.026 mg/L (based on compound-induced lesions in the nasal turbinates)
28-d NOAEL (local irritation) < 0.026 mg/L

Reproductive Toxicity

In a combined reproductive/developmental toxicity screening test, Sprague-Dawley rats were administered methane
sulfonic acid daily via gavage at 250, 500, or 1000 mg/kg-bw/day. Males were dosed four weeks prior to mating,
two weeks during the mating period and until sacrifice. Females were dosed four weeks prior to mating, two weeks
during the mating period, through gestation (3 weeks), lactation until day 4 post partum (pp) and until sacrifice (day
5 pp). No mortality was seen. Clinical signs in males included ptyalism (excessive salivation) at 1000 mg/kg-
bw/day. There were no treatment-related effects on body weights, organ weight changes or histopathology.

Methane sulfonic acid did not affect male and female reproductive performance or the progeny of the parental rats
up to 1000 mg/kg-bw /day.

LOAEL (parental/reproductive toxicity) >1000 mg/kg-bw/day (highest dose tested)

NOAEL (parental/reproductive toxicity) = 1000 mg/kg-bw/day

Developmental Toxicity

(1)	Female rats (25/dose) were given daily oral gavage doses of methane sulfonic acid at 0, 25, 100 or 400 mg/kg-
bw/day from gestation day 6 through 15 (GD 6-15). Based on the results of this study, the NOAEL was determined
to be 400 mg/kg-bw/day for maternal toxicity and developmental toxicity.

NOAEL (maternal and developmental toxicity) = 400 mg/kg-bw/day (highest dose tested)

LOAEL (maternal and developmental toxicity) > 400 mg/kg-bw/day

(2)	Female rats (8/dose) were given daily oral gavage doses of the test substance at 0, 25, 50, 100, 200 or 300
mg/kg-bw/day from GD 5 through 15. Treatment-related clinical signs included rales, labored respiration and
gasping in the 100, 200 and 300 mg/kg/day groups. Findings of red material around the nose and/or mouth in the
100, 200 and 300 mg/kg/day groups often correlated with occurrences of the aforementioned respiratory
abnormalities. These findings appeared to be a function of the dosage concentration; rather the dosage level, as they
were observed with similar frequency in the 100, 200 and 300 mg/kg/day groups, each of which received the test
article at a concentration of 50 mg/mL. Slight mean body weight losses and reduction in food consumption occurred
in the 100, 200 and 300 mg/kg/day groups during GD 6-9 when evaluated on a group mean basis. Food
consumption was slightly reduced in the 100, 200 and 300 mg/kg/day groups during GD 6-9 when the group means
were compared with the control group mean. Maternal toxicity was observed at dose levels of 100, 200 and 300
mg/kg/day (administered at a concentration of 50 mg/mL), as evidenced by changes in the clinical condition of the
animals and inhibition of body weight gain and food consumption. No maternal toxicity was observed at dose levels
of 25 and 50 mg/kg/day (administered at a concentration of 25 mg/mL). No developmental toxicity was observed at
dose levels up to 300 mg/kg/day.

LOAEL (maternal toxicity) = 100 mg/kg-bw/day (based on clinical observations, body weight and food
consumption)

NOAEL (maternal toxicity) = 50 mg/kg-bw/day

NOAEL (developmental toxicity) = 300 mg/kg-bw/day (highest dose tested)

LOAEL (developmental toxicity) > 300 mg/kg-bw/day

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Genetic Toxicity - Gene Mutation
In vitro

(1) In an Ames test, Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and TA1538 were exposed to
100, 500, 1000, 2500 or 5000 (ig/plate methane sulfonic acid. No increase in revertant colonies was seen up to the
cytotoxicity threshold (5000 (ig/plate) in the presence or absence of metabolic activation. Response to positive
controls was not indicated.

Methane sulfonic acid was not mutagenic in this assay.

(2) Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 and TA1538 were exposed to 10, 32, 100, 316
or 1000 (ig/plate methane sulfonic acid in the presence and absence of metabolic activation. Inhibition of growth,
observed as thinning of the background lawn of non-revertant cells, and reduction in colony numbers occurred in all
strains following exposure to 1000 (ig/plate. Response to positive controls was not indicated.

Methane sulfonic acid was not mutagenic in this assay.

(3) Salmonella typhimurium strains TA100 and TA1535 were exposed to 384.4 to 12,300 (ig/plate methane sulfonic
acid in the presence and absence of metabolic activation. Positive controls produced an appropriate response.
Methane sulfonic acid was not mutagenic in this assay.

Genetic Toxicity - Chromosomal Aberrations

In vivo

Male and female mice (5/sex/dose) were administered 0, 20, 100 or 500 mg/kg-bw methane sulfonic acid by gavage
for 24, 48, or 72 hours. From the protocol description, it is not clear if animals were dosed once or more than once.
Three males at the 500 mg/kg-bw dose were excluded from the analysis because they died or were sacrificed prior to
conclusion of the test. All surviving male mice of the 500 mg/kg-bw group showed piloerection; one animal also
exhibited rales. One female also presented rales. The positive control produced a significant increase in
micronucleated polychromatic erythrocytes. Methane sulfonic acid was negative for induction of micronuclei.
Methane sulfonic acid did not induce chromosomal aberrations in this assay.

Additional Information

Irritation

Methane sulfonic acid is corrosive to skin and highly corrosive to eyes.

Sensitization

Thirty guinea pigs were exposed dermally via topical application to 0.3 mL of methane sulfonic acid (treated group)
or with the vehicle (control group) for 6 hours on day 1, day 7 and day 14. On day 28, all the animals received 0.3
mL of the test substance at the concentration of 25% in their right flank for 24 hours. None of the test animals
responded with a skin grade that was suggestive of sensitization.

Methane sulfonic acid was not a sensitizer in this assay.

Conclusion: Methane sulfonic acid showed moderate acute oral toxicity to rats and moderate acute dermal toxicity
to rabbits. Methane sulfonic acid is corrosive to mouse skin, extremely corrosive to the eye and none of the test
animals responded with a skin grade that would have been suggestive of sensitization. Following repeated nose-
only aerosol exposure of rats for 4 weeks, exposure-related clinical signs consisted of rales and an increased
incidence of yellow matting on various body surfaces. A transient reduction in mean body weight gain of high-
exposed males during the first week of exposure was observed. Test substance-related microscopic findings were
observed in the nasal turbinates of the high-exposure group rats that were found dead and in all treated groups at
study week 4 and after 2 weeks recovery period. No effects were seen for parental toxicity or reproductive
performance in a combined reproductive/toxicity screening test. Available developmental toxicity data showed
maternal toxicity but no developmental effects were evident. Methane sulfonic acid was not mutagenic and did not
induce chromosomal aberrations.

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The potential health hazard of methane sulfonic acid is moderate based on the results of the repeated-dose toxicity
and maternal toxicity observed in the developmental toxicity tests.

4. Hazard Characterization

The evaluation of available aquatic toxicity data for fish, aquatic invertebrates and aquatic plants indicates the
potential acute hazard of methane sulfonic acid to aquatic organisms is moderate.

Methane sulfonic acid showed moderate acute oral toxicity to rats and moderate acute dermal toxicity to rabbits.
Methane sulfonic acid is corrosive to mouse skin, extremely corrosive to the eye and none of the test animals
responded with a skin grade that would have been suggestive of sensitization. Following repeated nose-only aerosol
exposure of rats for 4 weeks, exposure-related clinical signs consisted of rales and an increased incidence of yellow
matting on various body surfaces. A transient reduction in mean body weight gain of high-exposed males during the
first week of exposure was observed. Test substance-related microscopic findings were observed in the nasal
turbinates of the high-exposure group rats that were found dead and in all treated groups at study week 4 and after 2
weeks recovery period. No effects were seen for parental toxicity or reproductive performance in a combined
reproductive/toxicity screening test. Available developmental toxicity data showed maternal toxicity but no
developmental effects were evident. Methane sulfonic acid was not mutagenic and did not induce chromosomal
aberrations.

The potential health hazard of methane sulfonic acid is moderate based on the results of the repeated-dose toxicity
and maternal toxicity observed in the developmental toxicity tests.

5. Data Gaps

No data gaps were identified under the HPV Challenge Program.

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APPENDIX

Summary Table of the Screening Information Data Set
as submitted under the U.S. HPV Challenge Program

Endpoints

SPONSORED CHEMICAL
Methane sulfonic acid
(75-75-2)

Structure

0

0=S OH

ch3

Summary of Physical-Chemical Properties and Environmental Fate Data

Melting Point (°C)

19

Boiling Point (°C)

300 (e)

200-225 (Decomposition temperature)

Vapor Pressure
(hPa at 25°C)

5.71 xlO"4 (extrapolated)

Log K„w

-2.38

Water Solubility
(mg/L at 25°C)

lxl 0+6 @ 20 °C

Direct Photodegradation

39 d

Indirect (OH ) Photodegradation
Half-life (t1/2)

—

Stability in Water (Hydrolysis) (ti/2)

Not expected to undergo hydrolysis in the environment

Fugacity
(Level III Model)

Air(%)
Water (%)
Soil (%)
Sediment(%)

<1
40.7
59.2
<1

Biodegradation at 28 days (%)

100

Readily biodegradable

Summary of Environmental Effects - Aquatic Toxicity Data

Fish

96-h LCS0 (mg/L)

73 mg A.I./L

Aquatic Invertebrates
48-h ECS0 (mg/L)

1.7 (24-h)

Aquatic Plants
72-h ECS0 (mg/L)

(growth)
(biomass)

16 mg A.I./L
14 mg/A.I./L

9

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Summary Table (if the Screening Information Data Set
as submitted under the U.S. HPV Challenge Program

Eml points

SPONSORED CHEMICAL
Methane sulfonic acid
(75-75-2)

Summary of Human Health Data

Acute Oral Toxicity
LDS0 (mg/kg-bw)

750 (male rat)
461 (female rat)
649 (combined)

Acute Dermal Toxicity
LDS0 (mg/kg-bw)

> 1000 mg/kg-bw (rabbit)

Acute Inhalation Toxicity
LCS0 (mg/L)

-

Repeated-Dose Toxicity

(mg/L/day)

NOAEL/LOAEL

NOAEL (systemic toxicity) = 0.026 (28-d)
LOAEL (systemic toxicity) = 0.073
NOAEL (local irritation) < 0.026
LOAEL (local irritation) = 0.026

Reproductive Toxicity
(mg/kg-bw/day)
NOAEL/LOAEL

NOAEL(parental/reproductive toxicity) = 1000
LOAEL (parental/reproductive toxicity) > 1000

Developmental Toxicity
(mg/kg-bw/day)
NOAEL/LOAEL

Maternal
NOAEL
LOAEL

50
100



Developmental
NOAEL
LOAEL

300
>300

Genetic Toxicity - Gene Mutation
In vitro

Negative

Genetic Toxicity - Chromosomal Aberrations
In vivo

Negative

Additional Information





Skin Irritation
Eye Irritation
Sensitization



Corrosive
Highly corrosive
Not a sensitizer

- indicates endpoint was not addressed for this chemical.

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