Sampling and Analysis Plan (SAP) Template Tool for Addressing Environmental Contamination by Pathogens Technical Lead: Prepared By: Date: ------- Sampling and Analysis Plan Sampling and Analysis Plan Approvals SAP Project Number: Task Number: Research Effort: Other Tracking IDs: If Intramural or Extramural, EPA Approvals Name and Title: Signature/Date: Name and Title: Signature/Date: Name and Title: Signature/Date: If Extramural, Contractor Approvals Name and Title: Signature/Date: Name and Title: Signature/Date: Name and Title: Signature/Date: ŁEPA i ------- Sampling and Analysis Plan Distribution List Name: Email: Organization: ŁEPA ------- Sampling and Analysis Plan Revision History Status: Revision #: Date: Point of Contact: Revision: ŁEPA ------- Sampling and Analysis Plan Acronym List Acronym: Meaning: ŁEPA iv ------- Sampling and Analysis Plan Table of Contents Sampling and Analysis Plan Approvals i Distribution List ii Revision History iii Acronym List iv Table of Contents v 1 Background 1 1.1 General Data Collection Activity Description 1 1.2 Sampling Phase and Pathogen 1 Table 1.1 Pathogen Characterization 2 1.3 Goal 3 Table 1.2 Defining Intended Use, Outcomes, and Determination Statements 3 1.4 Site Information and History 4 1.4.1 Site Condition 4 1.4.2 Prior Actions Taken on the Site 4 1.4.3 Lines of Evidence 5 2 Agency Coordination and Key Personnel 6 2.1 Roles and Responsibilities of Key Agencies, Advisory Groups, and Laboratories 6 2.1.1 Federal Bureau of Investigation (FBI) 6 2.1.2 Centers for Disease Control and Prevention (CDC) 6 2.1.3 State and Local Public Health 6 2.1.4 Other Federal, State or Local Agencies 6 2.1.5 Laboratory Response Network (LRN) 6 2.1.6 Environmental Response Laboratory Network (ERLN) 7 2.1.7 Other Laboratories 7 2.2 Key Personnel 8 2.2.1 List of Key Personnel Involved in Planning Activities 9 2.2.2 List of Key Personnel Responsible for Data Collection and Management 10 2.2.3 Available Technical Expertise Teams 12 Table 2.1 Available Laboratory Resources 13 3 Boundaries of the Data Collection Activities 15 3.1 Operation Schedule and Relevant Deadlines 15 3.2 Regulatory/Jurisdictional Requirements 16 3.3 Hazard Characteristics 16 3.4 Practical Constraints 16 3.5 Location: Spatial and Contextual 16 3.5.1 Priority and Exclusion Zones 16 3.5.2 Conceptual Model 17 3.6 Scale for the Decision or Estimate 17 3.7 Budget Constraints 18 3.8 Other General Resources 18 3.9 Waste Facility Capacity and Considerations 18 4 Documentation and Forms 19 4.1 Sample Documentation 19 4.1.1 Sample Labels 19 4.1.2 Chain-of-Custody Forms 20 ŁEPA v ------- Sampling and Analysis Plan 4.2 Photographic and Video Documentation 20 4.3 Evidence Documentation 21 5 Sample Collection 22 5.1 General Description of Sample Collection Activities to be Conducted 22 Table 5.1 Sample Collection Planning Form 23 5.2 Sampling Supplies and Equipment 26 5.3 Sampling Approach 26 5.4 Sampling Locations 27 5.5 Plan for Obtaining Data 28 5.5.1 Sample Documentation Information 28 5.5.2 Sampling Diagrams 29 5.6 Sample Container and Equipment Decontamination 30 5.7 Sampling Team Configuration 30 5.8 Other Sample Collection Considerations 31 6 Quality Control Activities 32 6.1 Data Quality Indicators 32 Table 6.1 Data Quality Indicators 32 Table 6.2 Instrument Calibration 35 6.2 Field Quality Controls 36 6.3 Other QC Activities 36 7 Sample Transportation and Storage 37 7.1 Information on Sample Packaging and Shipping 37 Table 7.1 Sample Shipment Considerations 38 8 Processing and Analysis Protocols 39 8.1 Laboratory Reporting Requirements 39 Table 8.1 Analytical Protocols to Be Used 40 9 Data Reduction, Statistical Analysis, and Visualization 42 9.1 Data Review and Reduction 42 9.2 Data Assessment and Summary Statistics 42 9.3 Application of Results 43 Table 9.1 Defining Alternative Actions 44 10 Supplemental Plans 45 10.1 Quality Assurance Project Plan 45 10.2 Data Management Plan 45 10.3 Waste Management Plan 46 10.4 Health and Safety Plan 46 11 References 47 Appendix: Data Quality Objective Summary 49 For instructions on using this Sampling and Analysis Plan Template Tool, please refer to the User Guide. ŁEPA vi ------- Sampling and Analysis Plan 1 Background 1.1 General Data Collection Activity Description 1.2 Sampling Phase and Pathogen ŁEPA 1 ------- Table 1.1 Pathogen Characterization Pathogen: Pathogen Type: Select an item. Select an item. Select an item. Pathogen: Select an item. Select an item. Pathogen Type: Select an item. ŁEPA Sampling and Analysis Plan CDC Select Agent?: Fate and Transport: Q Yes Q No | Potential Exposure Pathway: Persistence/Stability: Select an item. CDC Select Agent?: Fate and Transport: Q Yes O No | Potential Exposure Pathway: Persistence/Stability: Select an item. 2 ------- 1.3 Goal Sampling and Analysis Plan Table 1.2 Defining Intended Use, Outcomes, and Determination Statements Intended Use of the Data: Principal Questions: Decision Statement(s) or Estimation Statement(s): Select an item. Select an item. Select an item. Select an item. ŁEPA 3 ------- Sampling and Analysis Plan 1.4 Site Information and History 1.4.1 Site Condition Please attach additional documents here and list the corresponding file names below: 1.4.2 Prior Actions Taken on the Site Attach File ŁEPA 4 ------- Sampling and Analysis Plan 1.4.3 Lines of Evidence Att3ch FN© Please attach additional documents here and list the corresponding file names below: ŁEPA 5 ------- Sampling and Analysis Plan 2 Agency Coordination and Key Personnel 2.1 Roles and Responsibilities of Key Agencies, Advisory Groups, and Laboratories 2.1.1 Federal Bureau of Investigation (FBI) 2.1.2 Centers for Disease Control and Prevention (CDC) 2.1.3 State and Local Public Health 2.1.4 Other Federal, State or Local Agencies 2.1.5 Laboratory Response Network (LRN) oEPA 6 ------- Sampling and Analysis Plan 2.1.6 Environmental Response Laboratory Network (ERLN) 2.1.7 Other Laboratories ŁEPA 7 ------- Sampling and Analysis Plan 2.2 Key Personnel Insert organizational chart, table or other documentation showing lines of reporting and communication here: If an image is not available, please attach the file here and type the corresponding file name below: Attach File ŁEPA ------- Sampling and Analysis Plan 2.2.1 List of Key Personnel Involved in Planning Activities Name: Role: Organization/Affiliation: Phone: Email: Name: Role: Organization/Affiliation: Phone: Email: Name: Role: Organization/Affiliation: Phone: Email: Name: Role: Organization/Affiliation: Phone: Email: Name: Organization/Affiliation: Email: Name: Role: Organization/Affiliation: Phone: Email: Name: Role: Organization/Affiliation: Phone: Email: ŁEPA 9 ------- Sampling and Analysis Plan Name: Role: Organization/Affiliation: Phone: Email: Name: Role: Organization/Affiliation: Phone: Email: Name: Role: Organization/Affiliation: Phone: Email: 2.2.2 List of Key Personnel Responsible for Data Collection and Management Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: ŁEPA 10 ------- Sampling and Analysis Plan Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: ŁEPA 11 ------- Sampling and Analysis Plan 2.2.3 Available Technical Expertise Teams Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: Name: Role: Organization/Affiliation: Expertise: Email: Phone: ŁEPA 12 ------- Sampling and Analysis Plan Table 2.1 Available Laboratory Resources Laboratory 1 Name: Laboratory Address/Phone: Shipping Address/Phone: Laboratory Contacts: Contract on File?: Qyes Oo Sample Type Capability: Analysis Capability: ERLN or LRN Member Lab?: Biosafety Level: Select an item. Select an item. Select an item. Laboratory 2 Name: Laboratory Address/Phone: Shipping Address/Phone: Laboratory Contacts: Contract on File?: Q Yes Q No Sample Type Capability: Analysis Capability: ERLN or LRN Member Lab?: Biosafety Level: Select an item. Select an item. Select an item. ŁEPA 13 ------- Sampling and Analysis Plan Laboratory 3 Name: Laboratory Address/Phone: Shipping Address/Phone: Laboratory Contacts: Contract on File?: Q Yes Q No Sample Type Capability: Analysis Capability: ERLN or LRN Member Lab?: Biosafety Level: Select an item. Select an item. Select an item. Laboratory 4 Name: Laboratory Address/Phone: Shipping Address/Phone: Laboratory Contacts: Contract on File?: Qyes Qno Sample Type Capability: Analysis Capability: ERLN or LRN Member Lab?: Biosafety Level: Select an item. Select an item. Select an item. ŁEPA 14 ------- Sampling and Analysis Plan 3 Boundaries of the Data Collection Activities 3.1 Operation Schedule and Relevant Deadlines Operation Schedule Item: Deadline: Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA 15 ------- Sampling and Analysis Plan 3.2 Regulatory/Jurisdictional Requirements 3.3 Hazard Characteristics 3.4 Practical Constraints 3.5 Location: Spatial and Contextual 3.5.1 Priority and Exclusion Zones Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA 16 ------- 3.5.2 Conceptual Model Sampling and Analysis Plan Please attach additional documents here and list the corresponding file names below: Attach File 3.6 Scale for the Decision or Estimate Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA 17 ------- Sampling and Analysis Plan 3.7 Budget Constraints 3.8 Other General Resources 3.9 Waste Facility Capacity and Considerations ŁEPA 18 ------- Sampling and Analysis Plan 4 Documentation and Forms 4.1 Sample Documentation Please attach additional documents here and list the corresponding file names below: Attach File 4.1.1 Sample Labels Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA 19 ------- Sampling and Analysis Plan 4.1.2 Chain-of-Custody Forms Please attach additional documents here and list the corresponding file names below: 4.2 Photographic and Video Documentation Attach File Attach Fil© Please attach additional documents here and list the corresponding file names below: ŁEPA 20 ------- Sampling and Analysis Plan 4.3 Evidence Documentation Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA 21 ------- Sampling and Analysis Plan 5 Sample Collection 5.1 General Description of Sample Collection Activities to be Conducted ŁEPA 22 ------- Sampling and Analysis Plan Table 5.1 Sample Collection Planning Form Please attach your Sample Planning Form(s) and/or enter your Sample Planning Form(s) information below. Attach File Sample Type: Sample Matrix: | Sample Collection Protocol to be Used: Wetting Solution Used: Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Specific Location(s): Location ID Numbers (individual or range of numbers): Preservation Required: Collection Containers (number, size, type): Samples Accepted by Laboratory?: Select an item. Q Yes Q No Sample Type: Sample Matrix: | Sample Collection Protocol to be Used: Wetting Solution Used: Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Specific Location(s): Location ID Numbers (individual or range of numbers): Preservation Required: | Collection Containers (number, size, type): Samples Accepted by Laboratory?: Select an item. Q Yes Q No ŁEPA 23 ------- Sampling and Analysis Plan Sample Type: Sample Matrix: Sample Collection Protocol to be Used: Wetting Solution Used: Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Specific Location(s): Location ID Numbers (individual or range of numbers): Preservation Required: Collection Containers (number, size, type): Samples Accepted by Laboratory? Select an item. O Yes O No Sample Type: Sample Matrix: Sample Collection Protocol to be Used: Wetting Solution Used: Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Specific Location(s): Location ID Numbers (individual or range of numbers): Preservation Required: Collection Containers (number, size, type): Samples Accepted by Laboratory?: Select an item. Q Yes Q No ŁEPA 24 ------- Sampling and Analysis Plan Sample Type: Sample Matrix: Sample Collection Protocol to be Used: Wetting Solution Used: Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Specific Location(s): Location ID Numbers (individual or range of numbers): Preservation Required: Collection Containers (number, size, type): Samples Accepted by Laboratory?: Select an item. Q Yes Q No Sample Type: Sample Matrix: Sample Collection Protocol to be Used: Wetting Solution Used: Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Specific Location(s): Location ID Numbers (individual or range of numbers): Preservation Required: Collection Containers (number, size, type): Samples Accepted by Laboratory?: Select an item. O Yes O No ŁEPA 25 ------- Sampling and Analysis Plan 5.2 Sampling Supplies and Equipment Please attach additional documents here and list the corresponding file names below: Attach File 5.3 Sampling Approach Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA 26 ------- Sampling and Analysis Plan 5.4 Sampling Locations Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA ------- Sampling and Analysis Plan 5.5 Plan for Obtaining Data 5.5.1 Sample Documentation Information Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA 28 ------- Sampling and Analysis Plan 5.5.2 Sampling Diagrams Insert Sampling Diagram image here: Please attach additional documents here and list the corresponding file names below: ŁEPA 29 ------- Sampling and Analysis Plan 5.6 Sample Container and Equipment Decontamination 5.7 Sampling Team Configuration Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA 30 ------- Sampling and Analysis Plan 5.8 Other Sample Collection Considerations ŁEPA 31 ------- Sampling and Analysis Plan 6 Quality Control Activities 6.1 Data Quality Indicators Table 6.1 Data Quality Indicators Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: ŁEPA 32 ------- Sampling and Analysis Plan Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: ŁEPA 33 ------- Sampling and Analysis Plan Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: Parameter: Measurement Method: QA/QC Check: Frequency: Acceptance Criteria: Corrective Action: ŁEPA 34 ------- Sampling and Analysis Plan Table 6.2 Instrument Calibration Equipment: Calibration/Certification: Tolerance: Frequency of Calibration: ŁEPA 35 ------- Sampling and Analysis Plan 6.2 Field Quality Controls 6.3 Other QC Activities ŁEPA 36 ------- Sampling and Analysis Plan 7 Sample Transportation and Storage 7.1 Information on Sample Packaging and Shipping ŁEPA 37 ------- Sampling and Analysis Plan Table 7.1 Sample Shipment Considerations Sample Type: Shipping Temperature: Sample Packaging: Transport Container Labels: Mode of Sample Transport: Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. Select an item. ŁEPA 38 ------- Sampling and Analysis Plan 8 Processing and Analysis Protocols 8.1 Laboratory Reporting Requirements Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA 39 ------- Sampling and Analysis Plan Table 8.1 Analytical Protocols to Be Used Sample Type: Laboratory Selected for Analysis: Sample Processing Procedure: Analytical Procedure: Select an item. Maximum Holding Time and Storage Temperature: Quantification and/or Detection Limit: Reporting Units: Select an item. Sample Type: Laboratory Selected for Analysis: Sample Processing Procedure: Analytical Procedure: Select an item. Maximum Holding Time and Storage Temperature: Quantification and/or Detection Limit: Reporting Units: Select an item. Sample Type: Laboratory Selected for Analysis: Sample Processing Procedure: Analytical Procedure: Select an item. Maximum Holding Time and Storage Temperature: Quantification and/or Detection Limit: Reporting Units: Select an item. Sample Type: Laboratory Selected for Analysis: Sample Processing Procedure: Analytical Procedure: Select an item. Maximum Holding Time and Storage Temperature: Quantification and/or Detection Limit: Reporting Units: Select an item. ŁEPA 40 ------- Sampling and Analysis Plan Sample Type: Laboratory Selected for Analysis: Sample Processing Procedure: Analytical Procedure: Select an item. Maximum Holding Time and Storage Temperature: Quantification and/or Detection Limit: Reporting Units: Select an item. Sample Type: Laboratory Selected for Analysis: Sample Processing Procedure: Analytical Procedure: Select an item. Maximum Holding Time and Storage Temperature: Quantification and/or Detection Limit: Reporting Units: Select an item. Sample Type: Laboratory Selected for Analysis: Sample Processing Procedure: Analytical Procedure: Select an item. Maximum Holding Time and Storage Temperature: Quantification and/or Detection Limit: Reporting Units: Select an item. Sample Type: Laboratory Selected for Analysis: Sample Processing Procedure: Analytical Procedure: Select an item. Maximum Holding Time and Storage Temperature: Quantification and/or Detection Limit: Reporting Units: Select an item. ŁEPA 41 ------- Sampling and Analysis Plan 9 Data Reduction, Statistical Analysis, and Visualization 9.1 Data Review and Reduction 9.2 Data Assessment and Summary Statistics ŁEPA 42 ------- Sampling and Analysis Plan 9.3 Application of Results ŁEPA 43 ------- Sampling and Analysis Plan Table 9.1 Defining Alternative Actions Principal Questions / Decision or Estimation Statement(s): Results of Data Collection Alternative Actions 44 ------- Sampling and Analysis Plan 10 Supplemental Plans 10.1 Quality Assurance Project Plan Please attach additional documents here and list the corresponding file names below: Attach File 10.2 Data Management Plan Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA 45 ------- Sampling and Analysis Plan 10.3 Waste Management Plan Please attach additional documents here and list the corresponding file names below: Attach File 10.4 Health and Safety Plan Please attach additional documents here and list the corresponding file names below: Attach File ŁEPA 46 ------- Sampling and Analysis Plan 11 References ŁEPA 47 ------- Sampling and Analysis Plan ŁEPA ------- Sampling and Analysis Plan Appendix: Data Quality Objective Summary Data Quality Objective Summary Table STEP 1. State the Problem General Data Collection Activity Description 1.1 General Data Collection Activity Description Sampling Phase and Pathogen 1.2 Sampling Phase and Pathogen Site Information and History 1.4 Site Information and History STEP 2. Identify the Goal of the Sampling and Analysis Activities (estimation statement) Goals 1.3 Goal Table 9.1 Defining Alternative Actions STEP 3. Identify the Information Inputs Sample Collection Table 5.1 Sample Collection Planning Form Plan for Obtaining Data 5.5 Plan for Obtaining Data Analytical Protocols Table 8.1 Analytical Protocols to be Used STEP 4. Define the Boundaries of the Sampling and Analysis Activities Regulatory and Jurisdictional Requirements 3.2 Regulatory/Jurisdictional Requirements Practical Constraints 3.4 Practical Constraints Location: Spatial and Contextual 3.5 Location: Spatial and Contextual 3.5.1 Priority and Exclusion Zones 3.5.2 Conceptual Model Scale of the Decision or Estimate 3.6 Scale for the Decision or Estimate STEP 5. Develop the Analytical Approach Data Review and Reduction 9.1 Data Review and Reduction Data Assessment and Summary Statistics 9.2 Data Assessment and Summary Statistics Application of Results 9.3 Application of Results STEP 6. Specify the Performance and Acceptance Criteria Data Quality Indicators 6.1 Data Quality Indicators Instrument Calibration Table 6.2 Instrument Calibration Field Quality Controls 6.2 Field Quality Controls STEP 7. Optimize the Design Sampling Approach 5.3 Sampling Approach Sample Documentation Information 5.5.1 Sample Documentation Information Sample Collection Planning Form Table 5.1 Sample Collection Planning Form ŁEPA 49 ------- |