\W
UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
NATIONAL CENTER FOR ENVIRONMENTAL ASSESSMENT
WASHINGTON, DC 20460
OJ
a
/y	r-\
•/
December 6, 2013
MEMORANDUM
OFFICE OF
RESEARCH AND DEVELOPMENT
SUBJECT: CASAC Review of First External Review Draft Integrated Science Assessment for
Oxides of Nitrogen - Health Criteria
Designated Federal Officer
Clean Air Scientific Advisory Committee
EPA Science Advisory Board Staff Office (1400R)
The First External Review Draft Integrated Science Assessment (ISA) for Oxides of Nitrogen -
Health Criteria prepared by the Environmental Protection Agency's (EPA) National Center for
Environmental Assessment - Research Triangle Park Division (NCEA-RTP) as part of EPA's ongoing
review of the primary (health-based) national ambient air quality standards (NAAQS) for nitrogen
dioxide (N02) was released on November 22, 2013. Electronic copies are available for download at
http://www.eoa.gov/ncea. The draft ISA will be reviewed by the Clean Air Scientific Advisory
Committee (CASAC) N02 Primary NAAQS Review Panel at a public meeting to be held March 12-13,
2014. We are in the process of distributing the draft ISA for Oxides of Nitrogen to the CASAC Oxides of
Nitrogen Panel. I am requesting that you forward our charge to the CASAC Oxides of Nitrogen Panel.
The purpose of the draft ISA is to identify, evaluate, and summarize scientific information on the
health effects associated with gaseous oxides of nitrogen. The ISA is intended to "accurately reflect the
latest scientific knowledge useful in indicating the kind and extent of identifiable effects on public health
which may be expected from the presence of [a] pollutant in ambient air" (Clean Air Act, Section 108; 42
U.S.C. 7408). This first external review draft ISA integrates the scientific evidence for review of the
primary (health-based) NAAQS forN02 and provides draft findings, conclusions, and judgments on the
strength, coherence, and plausibility of the evidence. The Preamble presents the process for ISA
development, including aspects considered in judging the overall weight of evidence and framework for
causal determination. Criteria used to identify relevant studies for inclusion in the ISA are also described
in the Preamble. Chapter 1 provides an integrative summary and conclusions of this assessment. This
chapter is supported by detailed information on the relevant evidence available from the multiple
disciplines and approaches related to the causal framework (Preamble to the ISA); atmospheric chemistry,
ambient concentrations, and exposure to oxides of nitrogen (Chapter 2); dosimetry and modes of action
(Chapter 3); health effects of short-term exposure to oxides of nitrogen (Chapter 4); health effects of long-
term exposure to oxides of nitrogen (Chapter 5); and lifestages and populations potentially at increased
for health effects related to oxides of nitrogen (Chapter 6). The final ISA for Oxides of Nitrogen, in
conjunction with additional technical assessments, will provide the scientific basis for EPA's decision
regarding the adequacy of the primary NAAQS for N02 to protect human health.
FROM:	John Vandenberg, Ph.D. 1st
Director
National Center for Environmental Assessment
Research Triangle Park Division (B243-01)
TO:
Aaron Yeovv, M.P.H.
Internet Address (URL) • http://www.epa.gov
Recycled/Recyclable • Printed with Vegetable Oil Based Inks on Recycled Paper (Minimum 50% Postconsumer content)

-------
The purpose of this memo is to provide charge questions related to a number of important topics
addressed in the ISA. Following the CASAC and public review of the draft ISA, NCEA-RTP will
produce a second draft ISA, which will be released the summer of 2014.
Charge to the CASAC Oxides of Nitrogen Panel
EPA has aimed to succinctly present and integrate the policy-relevant scientific evidence for the
review of the N02 NAAQS while also sufficiently describing how scientific information was evaluated in
forming the conclusions presented. Previous panels have emphasized the importance of older studies and
concluded that if older studies are open to reinterpretation in light of newer data and/or they remain the
definitive works available in the literature, they should be discussed in detail to reinforce key concepts
and conclusions. In considering subsequent charge questions and recognizing an overall goal of producing
a clear and concise document, are there topics that should be added or receive additional discussion?
Similarly, are there topics for which discussion should be shortened or removed? Does the Panel have
opinions on how the document can be shortened without eliminating important and necessary content?
In addition, we ask the Panel to focus on the following specific questions in their review:
1.	The Executive Summary is intended to provide a concise synopsis of the key findings and
conclusions of the ISA for a broad range of audiences. Please comment on the clarity with which the
Executive Summary communicates the key information from the ISA. Please provide
recommendation on information that should be added or information that should be left for discussion
in the subsequent chapters of the ISA.
2.	Chapter 1 summarizes key information from the Preamble about the process for developing an ISA.
Chapter 1 also presents the integrative summary and conclusions from the subsequent detailed
chapters of the ISA for Oxides of Nitrogen and characterizes available scientific information on
policy-relevant issues.
a.	Please comment on the usefulness and effectiveness of the summary presentation. Please provide
recommendations on approaches that may improve the communication of key ISA findings to
varied audiences and the synthesis of available information across subject areas.
b.	What are the Panel's thoughts on the application of the Health and Environmental Research
Online (HERO) system to support a more transparent assessment process?
c.	To what extent does Chapter 1 communicate the key scientific information on sources,
atmospheric chemistry, ambient concentrations, exposure, and health effects of oxides of nitrogen
as well as at-risk lifestages and populations? What information should be added or is more
appropriate to leave for discussion in the subsequent detailed chapters?
d.	What are the Panel's thoughts on the rationale presented for forming causal determinations for
N02 exposure only and considering epidemiologic results for associations between NOx and
health effects in causal determinations for N02 (Sections 1.4.1 and 1.4.3)?
2

-------
e.	Based on individual Panel member recommendations from June 20131 on the Draft Plan for the
Development of the Integrated Science Assessment for Nitrogen Oxides - Health Criteria (May
2013)2, Chapter 1 presents an integrated evaluation of various epidemiologic lines of evidence
that inform the independent effects ofN02 exposure (Section 1.5). This section discusses
available information that is not necessarily included in the health effect chapters on potential
confounding by copollutants and other factors as well as the potential for N02 to serve primarily
as an indicator of traffic-related pollutants and traffic proximity. This discussion is in Chapter 1
because it integrates information across Chapters 2, 4, and 5. Please comment on the extent to
which this discussion is informative in describing how the evidence of independent effects of
N02 is evaluated in this ISA. Does the discussion accurately reflect the available evidence? If this
discussion is informative, what information could be added or removed to improve the
discussion. Should the discussion remain in Chapter 1 or should it be moved to another part of the
ISA?
f.	Please comment on the extent to which the discussion of various policy-relevant considerations is
clearly described and integrates relevant information (Section 1.6). Please identify any other
relevant information that would be useful to include.
3.	Chapter 2 describes scientific information on sources, atmospheric chemistry, air quality
characterization, and human exposure of oxides of nitrogen.
a.	To what extent is the information presented regarding characteristics of sources, chemistry,
monitoring concentrations, and human exposure accurate, complete, and relevant to the review of
the N02 NAAQS?
b.	To what extent are the analyses of air quality presented clearly conveyed, appropriately
characterized, and relevant to the review of the N02 NAAQS?
c.	How effective are the source category groupings and the discussion of source emissions in
understanding the importance and impacts of oxides of nitrogen from different sources on both
national and local scales?
d.	Please comment on the extent to which available information on the spatial and temporal trends
of ambient oxides of nitrogen at various scales has been adequately and accurately described.
e.	Please comment on the accuracy, level of detail, and completeness of the discussion regarding
exposure assessment and the influence of exposure error on effect estimates in epidemiologic
studies of the health effects ofN02.
4.	Chapter 3 characterizes scientific evidence on the dosimetry and modes of action for N02 and nitric
oxide (NO). Dosimetry and modes of action are bridged by reactions ofN02 with components of the
extracellular lining fluid and by reactions of NO with heme proteins, processes that play roles in both
uptake and biological responses.
a. Given the ubiquity of reactive substrates and reaction rate of N02 with these substrates, it appears
unlikely N02 itself will penetrate through the lung lining fluid to the epithelium (see Table 3-1).
Please comment of the adequacy of the discussion of N02 uptake and reactivity in the respiratory
tract.
1 The individual panel member comments are available at
http://vosemite.epa.gov/sab/sabproduct.nsf/08EF0A3789CDB 13 A85257B8E006A496E/$File/EPA-CASAC-13-
006+unsigned.pdf
2The draft plan for development of the ISA is available at
http://vosemite.epa.gov/sab/sabproduct.nsf/4620a620d0120f93852572410080d786/bc264e65792e015f85257b4a0Q7
128c6! QpenDocument
3

-------
b.	Since existing dosimetric models for N02 do not consider the probability of oxidants/cytotoxic
products reaching target sites, it was concluded that these models are inadequate for within or
cross species comparisons. Please comment on the validity of this conclusion and identify and
comment on the validity of any alternative conclusions.
c.	Please comment on the adequacy of the discussion of endogenously occurring N02 and NO and
their reaction products in comparison to that derived from ambient inhalation.
d.	To what extent are the discussion and integration of the potential modes of action underlying the
health effects of exposure to oxides of nitrogen presented accurately and in sufficient detail? Are
there additional modes of action that should be included in order to characterize fully the
underlying mechanisms of oxides of nitrogen?
5. Chapters 4 and 5 present assessments of the health effects associated with short-term and long-term
exposure to oxides of nitrogen, respectively. The discussion is organized by health effect category,
outcome, and scientific discipline.
a.	To what extent do the discussions in this chapter accurately reflect the body of evidence from
epidemiologic, controlled human exposure and toxicological studies?
b.	Please comment on the balance of discussion of evidence from previous and recent studies in
informing the causal determinations.
c.	Please comment on the adequacy of the discussion of the strengths and limitations of the evidence
in the text and tables within Chapters 4 and 5 and in the evaluation of the evidence in the causal
determinations.
d.	What are the views of the panel on the integration of epidemiologic, controlled human exposure,
and toxicological evidence, in particular, on the balance of emphasis placed on each source of
evidence? Please comment on the adequacy with which issues related to exposure assessment and
mode of action are integrated in the health effects discussion. Please provide recommendations on
information in other chapters of the ISA that would be useful to integrate with the health effects
discussions in these chapters.
e.	Please comment on the appropriateness of using experimental and epidemiologic evidence for
morbidity effects to inform the biological plausibility of total mortality associated with short-term
(Section 4.4) and long-term (Section 5.5) N02 exposure and in turn, to inform causal
determinations.
f.	Section 4.2.2 discusses the effect of short-term N02 exposure on airways responsiveness. This
section focuses primarily on an EPA meta-analysis developed for this ISA of airway
responsiveness data for individuals with asthma and secondarily on the potential of various
factors to affect airways hyperresponsiveness independently or in conjunction with N02 exposure
in controlled human exposure studies. This material presently is unpublished and we ask the
Panel to provide the peer review for the analysis, in particular, to comment on the appropriateness
of the methodology utilized for the meta-analysis, the conclusions reached based this analysis,
and its use in the draft ISA. With regard to factors potentially affecting airways responsiveness,
please comment on the adequacy of this discussion. Are there other modifying factors that should
be considered?
4

-------
g.	The 2008 ISA for Oxides of Nitrogen stated that one of the largest uncertainties was the potential
for health effects observed in association with N02 exposure to be confounded by correlated
copollutants. To what extent has evidence that informs independent effects of N02 been
adequately discussed in Chapters 4 and 5 and appropriately interpreted as reducing uncertainty
(for example, evaluation of copollutant model results)? Has the current draft ISA appropriately
considered recent epidemiologic findings regarding potential copollutant confounding in causal
determinations? Please provide comments specifically for respiratory effects, cardiovascular
effects, and total mortality of short-term N02 exposure.
h.	To what extent is the causal framework transparently applied to evidence for each of the health
effect categories evaluated to form causal determinations? How consistently was the causal
framework applied across the health effect categories? Do the text and tables in the summaries
and causal determinations clearly communicate how the evidence was considered to form causal
determinations?
i.	What are the views of the panel regarding the clarity and effectiveness of figures and tables in
conveying information about the consistency of evidence for a given health endpoint? In
particular, was the use of the tables and figures in both the text and online in the HERO database
effective in providing additional information on the studies evaluated? Are there tables and
figures in the ISA that would be more appropriate to include as a resource in the HERO database?
6. Chapter 6 evaluates scientific information and presents conclusions on factors that may modify
exposure to N02, physiological responses to N02 exposure, or risk of health effects associated with
N02 exposure. Consistent with the ISAs for ozone and lead, conclusions on these at-risk factors
inform at-risk lifestages and populations.
a.	How effective are the categories of at-risk factors in providing information on potential at-risk
lifestages and populations? Is there information available on other key at-risk factors that is not
included in the first draft ISA and should be added?
b.	To what extent do the discussions in this chapter accurately reflect the body of available evidence
from epidemiologic, controlled human exposure, and toxicological studies, including the extent to
which evidence indicates that the effects ofN02 exposure are independent of other traffic-related
copollutants?
c.	Please comment on the consistency and transparency with which the framework for drawing
conclusions about at-risk factors has been applied in this ISA.
d.	To what extent is available scientific evidence on factors that modify exposure to N02 discussed
in the chapter and adequately considered in conclusions for at-risk lifestages or populations?
We look forward to discussing these issues with the CAS AC Oxides of Nitrogen Panel at our
upcoming meeting. Should you have any questions regarding the draft ISA for Oxides of Nitrogen, please
feel free to contact Dr. Steven Dutton (919-541-5035, dutton.steven@epa.gov) or Dr. Molini Patel (919-
541-1492, patel.molini@epa.gov).
cc: Aaron Yeow, SAB, OA
Kenneth Olden, ORD/NCEA
Reeder Sams, ORD/NCEA
Steven Dutton, ORD/NCEA
Molini Patel, ORD/NCEA
Mary Ross, ORD/NCEA
Deirdre Murphy, OAR/OAQPS
Erika Sasser, OAR/OAQPS
Beth Hassett-Sipple, OAR/OAQPS
5

-------