Comparison Table of Acceptance Criteria for EPA Method TO-15A (September 2019) to NATTS
Technical Assistance Document (TAD) Revision 3 (October 2016)

May 27, 2020

TO-15A

NATTS TAD Rev 3

Parameter

Description and Details

Required Frequency

Acceptance Criteria

Zero-air challenge of
analytical instrument

systems

Test of instrumentation to
demonstrate cleanliness
(positive bias) by analyzing
humidified zero air; performed
by connecting the clean
humidified gas sample to the
preconcentrator to verify that
the analytical instrument and
all connections are sufficiently
clean

At installation prior to initial
use of the instrument

Analysis must show that any
detected target compounds in
the zero-air challenge sample
are at response levels that are
expected to be < 20 pptv or
preferably not detected (see
Section 9.3.1)

Omitted

Known- standard
challenge of analytical
instrument systems

Test to demonstrate that the
analytical instrumentation
(preconcentrator and GC-MS
system) is not causing loss of
compounds (negative bias)

At installation prior to initial
use of the instrument

Verifies that all target
compounds are detected by the
system, that they respond
consistently upon repeated
injection, and that they exhibit
sufficient response to be
quantifiable at low
concentrations (see Section
9.3.2)

Omitted

Zero-air challenge of
auto samplers
associated with
analytical instrument

systems

After establishing the initial
calibration (ICAL), each port
of the autosampler is tested to
demonstrate cleanliness
(positive bias) by analyzing
humidified zero air; performed
by connecting the clean
humidified gas sample to the
port to verify that transfer lines
and all connections

Prior to initial use, upon
replacement of transfer lines, or
after analysis of potentially
contaminating samples

Each target VOC's
concentration should be < 20
pptv or preferably not detected
(see Section 9.3.3)

Omitted

Known- standard
challenge of
auto samplers
associated with
analytical instrument
systems

After establishing the ICAL,
each port of the autosampler is
tested with a reference standard
(approximately 100 to 500
pptv) to demonstrate that the
autosampler is not causing bias
(typically loss of compounds or
negative bias)

Prior to initial use and upon
replacement of transfer lines

Each target VOC's
concentration within ±15% of
theoretical concentration (see
Section 9.3.3)

Omitted

Canister leak check

Verification that canisters are
leak-free by performing a
pressure decay test of a canister
pressurized to approximately
203 kPa absolute (29.4 psia)
over the course of several days

Prior to initial use and
recommended periodically
thereafter (e.g., every 3 years)

Remove from service and
repair any canister that
exhibits a pressure change >
0.69 kPa/day (see Section
9.4.1)

Identical (< 0.1 psi/day)
[Section 4.2.4.1.1.1]

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Parameter

Description and Details

Required Frequency

Acceptance Criteria



Zero-air challenge of
canisters for
qualification

Test of canisters to determine
that they remain acceptably
clean (show acceptably low
positive bias) over the course
of a known time period,
typically 30 days or the
laboratory holding time, by
filling with humidified zero air
(not nitrogen)

Initially upon receipt in the
laboratory and every 3 years
thereafter

Upon initial analysis after a
minimum of 24 h and a
subsequent time period (e.g.,
30 days), each target VOC's
concentration < 20 pptv at
101.3 kPa absolute (14.7 psia)
(refer to Table 10-3 and
Section 9.4.2)

Strongly Recommended
Annually

Permits nitrogen

Must be < 3xMDL or 0.2
ppbv (200 pptv), whichever
is lower, at each timepoint

[Section 4.2.4.1.1.11

Known- standard
challenge of canisters
for qualification

Test of canisters to determine
bias by tilling with a known
reference standard
(approximately 100 to 500
pptv) prepared in humidified
zero air (not nitrogen) and
analyzing

Initially upon receipt in the
laboratory and every 3 years
thereafter

Upon initial analysis after a
minimum of 24 h and
subsequent analysis at 30 days
or typical laboratory holding
time, each target VOC's
concentration must remain
within ±30% of theoretical
concentration (see Section
9.4.3)

Strongly Recommended
Annually

Permits nitrogen

Concentration range 0.3 to 2
ppbv (300 to 2000 pptv)

Must recover within 70 to
130% of theoretical at each
timepoint

[Section 4.2.4.1.1.2]

Zero-air challenge of

sampling

devices/systems

Assessment of positive bias of
sampling system by collecting
humidified zero air through the
sampling device/system and
comparing it to the reference
sample collected upstream of
the sampling device/system

Prior to initial field deployment
and periodically thereafter (e.g.,
annually), following
maintenance (component
replacement), or after collection
of potentially contaminating
samples

Analysis must show that the
target compounds in the zero-
air challenge sample collected
through the sampling unit are
not > 20 pptv higher than the
concentration in the reference
sample (see Section 9.5.2)

To be conducted annually
following maintenance and
calibration

Permits nitrogen in addition
to zero air

Target VOCs in challenge
sample must not be >
3xMDL or 0.2 ppbv (200
pptv), whichever is lower,
more than the co-collected
reference sample

[Section 4.2.3.5.1]

Known- standard
challenge of sampling
devices/systems

Assessment of bias of sampling
system by collecting a known
reference standard
(approximately 100 to 500
pptv) through the sampling
device/system and comparing it
to the reference standard
collected upstream of the
sampling device/system

Prior to initial field deployment
and periodically thereafter (e.g.,
annually), following
maintenance (component
replacement), or after collection
of potentially contaminating
samples or damaging sample
matrices that may impact the
activity of the flow path
surfaces

Each target VOC's
concentration within ±15% of
concentrations in the reference
sample (see Section 9.5.3)

To be conducted annually
following maintenance and
calibration

Permits nitrogen in addition
to zero air as diluent gas

Target VOCs in challenge
sample must be within ±15%
difference of the
concentration measured in
the co-collected reference
sample

[Section 4.2.3.5.2]

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Parameter

Description and Details

Required Frequency

Acceptance Criteria

Purge gas check

Analysis of canister cleaning
purge gas to ensure
contaminants are acceptably
low

Verified upon initial setup and
in the event of changes in gas
sourcing or after the
replacement of scrubbers such
as hydrocarbon traps and
moisture traps, or following
maintenance of zero-air
generator

Each target VOC's
concentration < 20 pptv (see
Section 10.1.1)

Each target VOC < 3xMDL
or < 0.2 ppbv (200 pptv),
whichever is lower

[Section 4.2.4.2.3]

Canister cleaning batch
blank

Analysis of a sample of
humidified diluent gas in a
canister from a given batch of
clean canisters to ensure
acceptably low levels of VOCs
in the batch of cleaned
canisters

One or more canisters from
each batch of cleaned canisters
(chosen canister should
represent no more than eight
total canisters)

Alternatively, each canister
checked for cleanliness

Upon analysis 24 h after
tilling, each target VOC's
concentration should meet the
canister blank acceptance
criterion in Table 10-3 (i.e.,
<20 pptv at 101.3 kPa
absolute, 14.7 psia) (see
Section 10.2)

One canister per cleaning
batch (batch size not
specified, recommended as
one canister per ten cleaned).
At sample collection
pressure, each target VOC
< 3xMDL or < 0.2 ppbv (200
pptv), whichever is lower

[Section 4.2.4.2.41

Dilution blank (DB)

Canister filled with clean,
humidified diluent gas that is
used to dilute samples;
indicates that diluent gas and
dilution apparatus do not
contribute target VOCs to the
samples; the DB should not be
prepared through a dilution
system used for preparing
standards

Ideally one DB is prepared and
analyzed with each set of
samples that are diluted, and at
minimum one DB is prepared
and analyzed when source
and/or filters are changed

DB should be sufficiently
clean such that no positive bias
is imparted to the samples;
each target VOC's
concentration should be < 20
pptv (see Section 12.2).

Criteria not specified
[Section 4.2.7]

Holding time

Duration from end of sample
collection or canister
preparation to analysis

Each field-collected or
laboratory QC (standard or
blank) canister

< 30 days unless longer
stability can be demonstrated
(see Section 13.4)

As soon as possible after
collection, not to exceed 30
days

[Section 4.2.1]

MS tune check, as
applicable

May be accomplished by
injection of 1 to 2 ng
bromotiuorobenzene (BFB) for
tune verification of quadrapole
or ion trap MS detector

Prior to ICAL and prior to each
day's analysis

Abundance criteria for BFB
listed in Table 14-2 (see
Section 14.4.2)

Does not apply to time-of-
flight (TOF) or ion trap MS

Frequency: Prior to ICAL
and every 24 hours of
analysis thereafter.
Abundance criteria for BFB
in Table 4.2-2

[Section 4.2.8.5]

Retention time (RT)

RT of each IS and target
compound

All qualitatively identified
compounds and internal
standards

IS compounds within ±2 s of
their mean ICAL RTs (see
Section 15.1.1)

Target VOCs within ±2 s of
their mean ICAL RTs (see
Section 16.2)

IS compounds must be within
±0.33 minutes of the average
RT determined from the
ICAL

[Section 4.2.8.5.41

Target VOCs must be within
±0.06 relative retention time
(RRT) units of the average
from the ICAL

[Section 4.2.8.5.2.2]

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Parameter

Description and Details

Required Frequency

Acceptance Criteria



Internal standards (IS)

Deuterated or other compounds
not typically found in ambient
air co-analyzed with samples to
monitor instrument response
and assess matrix effects

Co-analyzed along with all
calibration standards, laboratory
QC samples, and field-collected
samples

Area response for each IS
compound preferably within
±30% of the average response
as determined from the ICAL
and may not exceed ±40% (see
Section 15.1.2)

Area response for each IS
compound must be within
±40% of the average
response determined from the
ICAL

[Section 4.2.8.5.4]

Initial calibration
(ICAL)

Analysis of a minimum of five
calibration levels (minimum
eight levels if using quadratic
regression) covering
approximately 20 to 5000 pptv

Before sample analysis;
following failed BFB tune
check (as applicable), failed IS
criteria, or failed CCV criteria;
or when changes/maintenance
to the instrument affect
calibration response

Average RRF < 30% RSD and
each calibration level within
±30% of theoretical
concentration; for quadratic or
linear curves, coefficient of
determination (r2) > 0.995, and
each calibration level within
±30% of theoretical
concentration (see Section
15.2.3)

Recommended calibration
range 0.03 to 5 ppbv (30 to
5000 pptv). Minimum 5
points, recommend more if
employing quadratic
regression model

Average RRF < 30% RSD
and each calibration level
within ±30% of theoretical
concentration; for quadratic
or linear curves, coefficient
of determination (r2) > 0.995,
and each calibration level
within ±30% of theoretical
concentration

[Section 4.2.8.5.2.2]

Second source
calibration verification
(SSCV)

Analysis of a secondary source
standard in the lower third of
the calibration curve to verify
ICAL accuracy for each target
analyte

Immediately after each ICAL

Measured concentrations of
VOCs should be within ±30%
of theoretical concentration
(see Section 15.3.1)

Concentration not specified

Frequency and acceptance
criteria identical

Additionally allows for RRF
of each compound to be
within ±30% of the ICAL
mean RRF

[Section 4.2.8.5.2.31

Continuing calibration
verification (CCV)

Analysis of a known standard
in the lower third of the
calibration curve to verify
ongoing instrument calibration
for each target analyte

Prior to analyzing samples in an
analytical sequence and at the
end of a sequence;
recommended after every 10
sample injections

Measured concentrations of
VOCs within ±30% of
theoretical concentration (see
Section 15.3.2)

Concentration not specified

Frequency is every 24 hours
of analysis following
successful calibration

Acceptance criteria identical

Additionally allows for RRF
of each compound to be
within ±30% of the ICAL
mean RRF

[Section 4.2.8.5.2.41

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Parameter

Description and Details

Required Frequency

Acceptance Criteria

Instrument blank (IB)

Analysis of an injection where
no sample or standard is
introduced to the
preconcentrator to preliminarily
demonstrate the carrier gas and
instrument are sufficiently
clean to begin analysis

Prior to ICAL and at the
beginning of an analytical
sequence

Each target VOC's
concentration should be < 20
pptv (see Section 15.3.3.1)

Recommended

Each target VOC < 3xMDL
or < 0.2 ppbv (200 pptv),
whichever is lower

[Section 4.2.8.5.2.2]

Method blank (MB)

Canister filled with clean,
humidified gas; indicates that
target VOCs and potential
interferences are at acceptably
low levels in the system as a
whole; the MB is to help assess
overall quality of the data

Prior to and following the ICAL
and prior to the initial daily
CCV/SSCV

This should demonstrate
acceptably low carryover in
the analytical system prior to
analysis of samples; each
target VOC's concentration
should generally be < 20 pptv
(see Section 15.3.3.2)

Frequency required once
each analysis batch of 20 or
fewer field collected samples

Each target VOC < 3xMDL
or < 0.2 ppbv (200 pptv),
whichever is lower

[Section 4.2.8.5.2.5]

Calibration blank (CB)

Canister filled with clean,
humidified diluent gas;
indicates that diluent gas and
dilution apparatus do not
contribute target VOCs, i.e.,
positive bias to the ICAL is
acceptably low; may also serve
as zero point in the ICAL

Prepare one CB with each set of
calibration standard canisters
and analyze with each ICAL

CB should be sufficiently
clean such that little or no
positive bias is imparted to the
calibration (see Section
15.3.3.3)

Omitted

Method precision

Duplicate samples: precision is
determined from the analyzed
concentrations of samples
collected simultaneously from
the same air mass using two
discrete canisters collected
through the same sampling
inlet (e.g., a rack-mounted
system that employs one inlet
to fill two canisters at the same
time; this determines the
precision of the sampling and
analysis processes

OR

Collocated samples: precision
is determined from the
analyzed concentrations of
samples collected
simultaneously from the same
air mass using two discrete
canisters collected through two
separate sampling inlets (e.g.,
two mechanical flow control
devices (MFCDs) that are
individually attached to two
canisters); this determines the
precision of the sampling and
analysis processes

Applicable to the collection of
samples: collect approximately
5% of total samples or
minimum of three samples

Precision < 25% relative
percent difference (RPD) of
target VOCs in the compared
samples when both
measurements are > fivefold
MDL (see Section 15.3.4)

For sites collecting precision
samples, must be 10% of
primary sample frequency

Acceptance criteria identical

[Section 4.2.2]

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Parameter

Description and Details

Required Frequency

Acceptance Criteria

Instrument precision

Precision is determined from
repeated analyses of a gas
sample from one canister;
replicate analyses are used to
determine precision of the
analysis processes and do not
provide information on
sampling precision

One replicate analysis with each
analytical sequence or 5% of
field samples in each analytical
sequence, whichever is greater

Precision < 25% RPD for
target VOCs when both
measurements are > fivefold
MDL (see Section 15.3.4)

Recommended as one per
analysis batch or one per 20
sample injections, whichever
is greater

Acceptance criteria identical

[Sections 4.2.2.2 and
4.2.8.5.2.51

Field blank

Canister filled with clean,
humidified diluent gas
transported to the field site(s)
with field collected samples;
indicates that sample handling
practices do not contaminate
samples

Optional, prepared for transport
with field-collected samples;
frequency determined by
method user

Each target VOC's
concentration should be
approximately 20 pptv or less
(see Section 15.3.5)

Omitted

Field spike

Canister filled with humidified
standard gas at a concentration
in the lower third of the
calibration curve and
transported to the field site(s)
with field collected samples;
indicates that sample handling
practices do not deteriorate
sample integrity

Optional, prepared for transport
with field-collected samples;
frequency determined by
method user

Measured concentrations of
VOCs within ±30% of
theoretical spiked
concentrations (see Section
15.3.5)

Omitted

Audit accuracy

Analysis of an independently
prepared audit standard to
determine analytical accuracy

Annually at a minimum

Within ±30% of accepted
reference value (see Section
15.3.6)

Audit performed through
NATTS PT program must
show bias within ±25% of
study NATTS Laboratory
mean

[Section 2.1.4.1]

Preconcentrator leak
check

Pressurize or evacuate the
canister connection to verify as
leak-free

Each canister connected to the
instrument prior to analysis

< 3.4 kPa (0.5 psi) change per
minute or as recommended by
the manufacturer (see Section
16.1.2)

Must be < 0.2 psi/minute or
meet manufacturer
specifications

[Section 4.2.8.5.2.1]

Method detection limit
(MDL)

Establishes the minimum
amount of a target analyte
distinguishable above
background with 99%
confidence; determined from
spiked canisters and MB
canisters

Annually at a minimum

MDLs are recommended to be
<20 pptv or should meet
program goals (see Table 17-2
for example MDLs)

Determined annually

Minimally 7 method blank
and 7 spiked canisters;
prepared over 3 separate
dates and analyzed over 3
separate dates

Determined MDLs must be
< NATTS MDL
Measurement Quality
Objective

[Section 4.2.5]

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Parameter

Description and Details

Required Frequency

Acceptance Criteria

MDL confirmation
sample

Known standard prepared at
approximately onefold to
fivefold the determined MDLsp
to confirm the determined
MDL is reasonable

Not required but recommended
for MDLs determined as the
MDLsp (and not for those
determined as the MDLb)

Recommended recovery
within 40% to 160% or other
in-house defined limits (see
Section 17.8)

Identical
[Section 4.1.3.1]

Compound
Identification

(Not listed in TO-15A
Table 18-1)

Criteria that must be met to
positively identify a target
compound

Refer to Section 16.2

Each target VOC

1.	Compound RT must be
within ±2s from ICAL
average RT

2.	At least one qualifier ion
must be within ±30%
relative abundance of the
average abundance of the
quantitation ion
established in the initial
calibration

3.	Signal-to-noise ratio >
3:1, preferably >5:1

4.	Quantitation ion and
qualifier ion must be co-
maximized (peak apexes
within 1 scan of each
other)

Permits positive identification
by experienced analyst when
any of these four criteria is not
met. Rationale for such
positive identification should
be documented and reported
data should be flagged to
indicate identification criteria
were not met

1.	Compound RT must be
within the assigned RT
window

2.	Does not specify
relative abundance,
otherwise identical

3.	S:N identical

4.	Co-maximization
identical

Permits analyst interpretation
of signal to noise and co-
maximization as well as
positive identification for
experienced analyst with
documented rationale

[Section 4.2.8.5.3]

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