United States Environmental Protection Agency	Office of Research and Development

National Exposure Research Laboratory
Research Abstract

Government Performance Results Act Goal: Sound Science

Significant Research Findings:

Stressor Identification Guidance

State and Tribal water quality experts are finding water bodies where
the fish, invertebrate, algae or plant communities (or other aquatic life)
have been detrimentally impacted by different single or multiple
causes. In many cases, the cause, or causes, of these biological
impairments have not yet been identified. The Stressor Identification
Guidance Document is designed to assist water quality managers in
identifying unknown causes of biological impairments in any type of
water body. Scientifically sound identification of the cause of water
quality impairment is an initial step in developing Total Maximum
Daily Loads (TMDLs), as required by Section 303(d) of the Clean
Water Act (CWA). The Stressor Identification Guidance Document
was published under the authority of Section 304(a)(2) of the CWA.

Research Approach The Stressor Identification Guidance Document provides a logical,

scientific process by which State, Tribal, and other water quality
experts can evaluate available information to identify the stressor(s)
causing biological impairments. A step-wise analytical process was
developed to determine the cause of an ecological impairment. Logic
steps in the causal identification process were derived from the
scientific literature including the areas of eco-epidemiology, human
epidemiology, forensics, symptomology, diagnosis, and general
philosophy of science and inductive inference.

The three main steps of the stressor identification process are (1) list
candidate causes of impairment, (2) analyze the evidence, and (3)
characterize the causes. The last step, characterizing the cause,
evaluates the evidence in three steps: elimination, diagnosis, and
strength of evidence. When evidence is adequate, investigators should
be able to use this guidance to successfully identify the likely cause(s).
This guidance will also help investigators identify where evidence is
weak or lacking and needs to be developed to enable successful
identification of the stressor(s).

Scientific Problem and
Policy Issues

Results and
Implications

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Once the causes of the biological impairments are identified, water
resource managers will be better able to locate the sources of the
stressor(s) and take management actions aimed at improving the
biological condition of the water body. This guidance is advisory in
nature and its use is not mandatory. As such this guidance does not
impose legally-binding requirements on the U.S. EPA, the States,
Tribes, industry, the public, or any other entity.

The U.S. EPA has published the Stressor Identification Guidance
Document under document number EPA-822-B-00-025, dated
December 2000. Paper copies can be obtained from the U.S. EPA
Water Resource Center by phone at (202) 260-7786, by e-mail at
center.water-resource@epa.gov. or through conventional mail by
sending a letter of request to U.S. EPA Water Resource Center, Ariel
Rios Building, 1200 Pennsylvania Ave., Washington, DC 20460.
Copies of the document may also be obtained from the U.S. EPA
National Center for Environmental Publications and Information
(NCEPI) by phone at (513) 489-8190 or through conventional mail at
11029 Kenwood Road, Cincinnati, OH 45242. The document and fact
sheet are also available on the U.S. EPA website at
http://www.epa.gov/OST/biocriteria.

Future Research	Several avenues of research continue to build on the foundation

established by the stressor identification process. Case studies are
being performed to demonstrate how the process works with both
simple and complex situations in a variety of ecosystems.

Associations and other types of evidence are being developed that can
be used in many causal analyses, for instance, the relationship between
excessive nutrients and sensitive biological endpoints such as aquatic
insects. This type of information can be used in many causal
determinations. A Causal Analysis, Diagnosis and Decision
Information System (CADDIS) is being planned that will allow routine
application of the process and put needed data and tools at the
fingertips of resource managers.

Research
Collaboration and
Publications

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Contacts for	Questions and inquiries can be directed to:

Additional

Information

Susan Cormier

U.S. EPA, Office of Research and Development
National Exposure Research Laboratory
26 W. Martin Luther King Dr.

Cincinnati, OH 45268

Phone: 513/569-7995
E-mail: cormier.susan@epa.gov

National Exposure Research Laboratory — October 2001


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