United States Environmental Protection Agency	Office of Research and Development

National Exposure Research Laboratory
Research Abstract

Government Performance Results Act (GPRA) Goal 2
Annual Performance Measure 71

Significant Research Findings:

Report on the Biological Condition of Mid-Atlantic Shallow Streams
and Deep Rivers Based on Macroinvertebrates as a Basis for

Management Action

Scientific	In 1995, the U.S. Environmental Protection Agency's (EPA) Office of Research

Problem and	and Development (ORD) formed a partnership with the EPA Region 3 Office to

Policy Issues	implement a research, monitoring, and assessment project in the Mid-Atlantic

Region of the U.S. This project was the Mid-Atlantic Integrated Assessment
(MAIA) and included forests, estuaries, and streams. The MAIA's mission was to
inject scientific knowledge into the decision-making process for the Mid-Atlantic
Region of the U.S. In 1997 and 1998, a probabilistic assessment of shallow
streams and deep rivers in the region was conducted and included collection of
fish, macroinvertebrates, and algae indicators. These data can provide a baseline
from which states and the region can monitor streams and deep rivers.

This study focused on evaluating the macroinvertebrate data for shallow streams
and deep rivers from the MAIA study for the purposes of assessing condition and
identifying macroinvertebrate indicators appropriate for each type of water body.
Macroinvertebrates are commonly used as indicators of condition in streams
because they have a relatively long lifespan compared to algae but are less mobile
than fish. For a portion of shallow streams, an existing multimetric
macroinvertebrate biotic integrity index (MBII) and the probabilistic design were
used to assess biological condition directly. In addition, a nonparametric risk
analysis was conducted using shallow stream data from the entire mid-Atlantic
region. This analysis identified abiotic factors that influence index scores, as well
as values of the individual metrics that comprise the MBII. For the much smaller
set of sites on deep rivers, there is no existing macroinvertebrate indicator
available. Instead, individual metrics were evaluated for the ability to reflect
impacts from various potential stressors at these sites.

The application of the MBII to shallow streams data from 1997 and 1998 in the
mid-Atlantic highlands part of the region estimated that approximately 19%
(66,215 km) of streams in that region are in good condition, 25% (86,374 km) are
in poor condition, and the rest (189,521 km) are in fair condition. The largest
proportion of sites in good condition and the smallest proportion of sites in poor

Research
Approach

Results and
Impact

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condition was found in the Northern Appalachians ecoregion. A relatively large
proportion of sites in Poor condition were found in the Piedmont, Valley, and
Western Appalachians ecoregions. Very small proportions of sites in Good
condition were found in the Valley and Central Appalachians ecoregions.

The risk analysis indicated that in-stream habitat was very influential to the
overall MBII score, but each component metric responded to a different set of
abiotic variables. It also showed that, in general, when only a few potential
abiotic risk factors were present at a site, the effect on macroinvertebrates tended
to be small, but risk of a negative biological impact increased rapidly at
intermediate numbers of risk factors.

Analysis of metrics for the deep rivers data showed that only a small number of
metrics correlate with physical habitat or water chemistry measures. Taxa
richness of chironomids and percent of individuals in the dominant five taxa were
correlated with several habitat variables, and percent of individuals as collector-
gatherers and intolerant taxa richness were correlated with several water
chemistry variables. Several macroinvertebrate metrics tested were related to
mean substrate size and may serve as potential indicators of sedimentation.

Further testing of these metrics is warranted to determine the extent of their utility
outside of the Mid-Atlantic region.

This project supports ORD's research to improve the quality of the nation's
waters under the Government Performance and Results Act (GPRA) Goal 2 Clean
and Safe Water. The report of this research (Annual Performance Measure (APM)
71) provides water resource managers with an illustration of the use of
bioindicators to assess condition and to identify potential stressors that may be
targeted for management action at the state and regional level. It illustrates the
necessity to consider the water body type, shallow streams versus deep rivers,
when selecting the indicators for assessment. As stated above, a new tool, a
nonparametric risk analysis, was applied to the shallow stream data in this
research project. Results of the nonparametric risk analysis identified potential
stressors to the macroinvertebrate communities of mid-Atlantic shallow streams.

Technical lead for development of this report was National Exposure Research
Laboratory (NERL) Ecological Exposure Research Division (EERD) staff
scientists. The design of the MAIA study on streams and rivers was a
collaborative effort between NERL and National Health and Environmental
Effects Research Laboratory NHEERL staff scientists. Sample collection and
processing were conducted through contractor support by SoBran, Inc. Analysis
of the data was conducted by NERL/EERD staff scientists, with contractor
assistance by SoBran, Inc.

Future Research This report will be provided to stakeholders in the Mid-Atlantic region for further
review and refinement to increase its utility as a management tool. The work
described here is part of the larger MAIA project, which is, in turn, a part of the
larger Environmental Monitoring and Assessment Program (EMAP). This
program is working to assess status and trends in waters across the U.S.

Research
Collaboration and
Research
Products

National Exposure Research Laboratory — October 2003


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Contacts for	Questions and inquiries can be directed to:

Additional	Joseph Flotemersch

Information	U.S. EPA, Office of Research and Development

National Exposure Research Laboratory
26 West Martin Luther King Dr.

Cincinnati, OH 45268
Phone: 513/569-7086
E-mail: flotemersch.joseph@epa.gov

National Exposure Research Laboratory — October 2003


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