United States Environmental Protection Agency	Office of Research and Development

National Exposure Research Laboratory
Research Abstract

Government Performance Results Act Goal: Clean and Safe Water and Sound Science

Significant Research Findings:

Landscape Indicators for Pesticides Study-
Mid-Atlantic Coastal Streams

Scientific Problem There is a growing interest and need for factual information about the

and Policy Issues	environmental condition of streams and watersheds at local, state, and

national levels. Until now, producing this information has been
expensive and often beyond the price range of those who could benefit
most. The Landscape Indicators for Pesticides Study-Mid-Atlantic
Coastal Streams (LIPS-MACS) demonstrates the "landscapes
approach," which extends the information gained from statistically
selected, ground-based monitoring sites by using remotely-sensed data
from satellites and applying principles from ecology, hydrology, and
geography. For example, coarse scale features such as elevation and
topography constrain finer scale features such as watersheds to behave
in a similar manner. The study focuses on pesticides and nutrients in
Mid-Atlantic freshwater, coastal streams. These parameters were
selected because excess pesticides and nutrients in the environment can
cause a variety of ecological and human-health effects. The occurrence
of pesticides in streams and groundwater has implications for Food
Quality Protection Act (FQPA) regulatory processes. Nutrients are
principal pollutants causing water quality problems according to the
Clean Water Act 305b listings for states nationwide. Total Maximum
Daily Load values for these pollutants are being developed for many
streams. The landscapes approach has the potential to rank all
watersheds in an area (not just those sampled) based on consistent
quantitative criteria and to evaluate land use management options using
projected future land use scenarios.

Research Approach The LIPS-MACS study is the first to utilize monitoring data collected

specifically for the purpose of developing landscape indicators. The
study approach incorporates a study design that enables data to be
collected for two purposes simultaneously, 1) to provide data to
develop landscape indicator statistical models and 2) to characterize
regional conditions and compare subregions within the study area. For
the Mid-Atlantic Coastal Streams, the objectives were to a) design a
field study for first-order streams and watersheds stratified by

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hydrogeologic subregions, and within each subregion provide a range of
land use conditions; b) select specific watersheds and associated
sampling locations; c) sample streams and nearby habitat one time in the
spring, 2000; d) analyze the data to develop landscape indicator
statistical models and to estimate pesticide and nutrient concentrations
to characterize the entire study area; and e) use the landscape indicator
models to estimate pesticide and nutrient concentrations for each of the
first-order watersheds in the Mid-Atlantic Coastal Plain, incorporating
these results into analyses developed in conjunction with local, state,
and federal stakeholders.

Results and	For the LIPS-MACS study, an unequal probability, stratified random

Implications	sampling design was used to select a network of 174 headwater streams

that provided both a gradient across percent developed land use/land
cover conditions and a statistically representative set of sampling sites
for the Coastal Plain region. Each hydrogeologic framework subregion
was represented by 23 - 25 sites. Water samples were collected in
Spring, 2000 during baseflow conditions for all streams and analyzed
for selected pesticides, pesticide degradates, nutrients, and major ions;
in addition, benthic community and habitat assessments were performed
and sediment samples were collected during the same time period. To
date, the LIPS-MACS study has successfully developed landscape
indicator statistical models for herbicides, nitrate + nitrite, and chloride
in small, fresh, headwater streams for the Mid-Atlantic Coastal Plain,
while simultaneously providing data to characterize regional conditions.
The landscape indicator statistical models were developed using
landscape and soil metrics and stepwise regression. These models
show that increased human land use is associated with increased
dissolved concentrations of herbicides, nutrients, and chloride for these
streams. Low concentrations of pesticides were present in over 80% of
the streams. The presence of herbicides in streams during baseflow
conditions indicates these chemicals are widespread in the shallow
ground water of the Coastal Plain. This shallow ground water is
sometimes used for drinking water in rural areas. Concentrations of
nitrogen and phosphorus exceeded the EPA-recommended ecoregional
nutrient levels of 0.71 mg/L for total nitrogen for 39% of the streams and
0.03 mg/L for more than 66% of the streams respectively. These
nutrient levels were developed by EPA to provide guidance to States
and Tribes for the protection and propagation of aquatic life and
recreation. Finally, comparison of the values for nitrate + nitrite
measured within each of the hydrogeologic subregions showed
significant differences between some subregions. This suggests that
different mitigation strategies may be needed for different areas to
achieve the same degree of water quality protection.

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Research	This study is a collaborative research project funded by the U.S.

Collaboration and Environmental Protection Agency's Office of Research and

Publications	Development and the U.S. Geological Survey. Within EPA's Office of

Research and Development, individuals are participating from the
National Exposure Research Laboratory Divisions in Las Vegas,
Nevada; Cincinnati, Ohio; and Athens, Georgia; and the National Health
and Environmental Effects Laboratory in Corvallis, Oregon. In the U.S.
Geological Survey, individuals are participating from the Maryland,
Delaware, and District of Columbia District, Water Resources
Division.

Pitchford, A.M., Neale, A.C., Ator, S.W., Ebert, D.W., Van Remortel, R.D., Denver,
J.M., Olsen, A., Subramanian, B., Kutz, F.W., Jones, K.B., Landscape
Approach for Assessing Stream Condition: Landscape Indicators for
Pesticide Study Mid-Atlantic Coastal Streams, (TIP # 01-126, APM 130,
Journal Article) A. Pitchford, PO. submitted to Environmental
Monitoring and Assessment, September 2001.

Kutz, F.W., Garibay, R., West, F., Bottimore, D., Perryman, T., and Orochena, S.

Maryland Agriculture and Your Watershed. EPA 903-R-00-009.

Pitchford, A.M., Denver, J.M., Olsen, A.R., Ator, S.W., Cormier, S., Nash, M.S., and
Mehaffey, M.H. Testing Landscape Indicators for Stream Condition
Related to Pesticides and Nutrients: Landscape Indicators for Pesticides
Study for Mid-Atlantic Coastal Streams (LIPS-MACS). EPA/600/R-
00/087

Ator, S.W., Denver, J.M., Pitchford, A.M. Developing landscape indicator models
for pesticides and nutrients in streams of the Mid-Atlantic Coastal Plain
(USGS Fact Sheet FS-157-00) A. Pitchford PO.

Future Research	Statistical analysis of the LIPS-MACS data is continuing, and analyses

of the benthic macroinvertebrate and sediment data will be incorporated
into the process. The landscape indicator models will be applied to all
at the 10,000 first-order watersheds in the Coastal Plain to predict their
current condition and to identify watersheds at risk. Landscape change
scenarios will be used to investigate the effects of urbanization. A
similar study in the Midwest is in the planning stages, and a study in
California will follow.

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Information

Contacts for	Questions and inquiries concerning the LIPS-MACS research can be

Additional	directed to Ann Pitchford for landscape indicators; Judith Denver for the

hydrogeologic framework, water quality sampling and water chemistry;
and Susan Cormier for benthic macroinvertebrate sample collection and
analysis.

Questions and inquiries can be directed to:

Ann M. Pitchford

US EPA, Office of Research and Development
National Exposure Research Laboratory
Las Vegas, NV 89193

Phone: 702/798-2366
E-mail: pitchford.ann@epa.gov

Judith M. Denver

US Geologic Survey, Maryland-Delaware-DC District
Dover, DE 19901

Phone: 302/734-2506
E-mail: jmdenver@usgs.gov

Susan M. Cormier

US EPA, Office of Research and Development
National Exposure Research Laboratory
Cincinnati, OH 45268

Phone: 513/569-7995
E-mail: cormier.susan@epa.gov

Federal funding for this research was administered under EPA
interagency agreement DW 149385351 with the U.S. Geological Survey
and under EPA off-site contracts 68-W5-0011 and 68-D-00-267 with
Lockheed Martin Environmental Services.

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