United States Environmental Protection Agency	Office of Research and Development

National Exposure Research Laboratory
Research Abstract

Significant Research Findings:

Theories, Concepts, and Approaches for the
Bioassessment of Non-wadeable Streams and Rivers

Scientific	The development of bioassessment techniques and protocols for nonwadeable

Problem and	rivers has progressed at a much slower pace than that for wadeable streams. In

Policy Issues	the past, methods used in wadeable streams were often applied in these larger

systems with little modification. In 1999, USEPA conducted a study to compare
several wadeable sampling methods being used in nonwadeable systems.

Published results of the study were structured to provide scientifically-sound
information to resource managers on the performance differences among the
methods. In some cases, the results of the study also identified the need for
development of new methods to address needs of the states, tribes, and regions.
This research has now been largely completed and is either published, or in the
process of being published in multiple formats to maximize outreach. However,
during presentations and workshops conducted throughout the United States and
discussions with EPA regional scientists and program offices, feedback was
received expressing the need for a final comprehensive document that presented
the material in the context of associated theories and concepts.

Research	This draft document provides a technical framework for assessing biological

Approach	condition and a scientific basis for decision-making regarding large, non-wadeable

rivers. The target readership is primarily program managers and technical staff
involved in the development and implementation of biological monitoring
programs for these aquatic resources. The document is intended to assist users in
establishing or refining protocols, including the specific methods related to field
sampling; laboratory sample processing; taxonomy; data entry, management, and
analysis; and final assessment and reporting. The combination of field-based
comparative studies and collaborative field tests involving State agency biologists,
Tribal members, and academic researchers was critical in assuring the resulting
protocols were consistent with user needs. Findings from these studies,
justification for follow-up research, and the performance of newly developed
methods are discussed. The protocols presented in this document are designed for
non-wadeable rivers (i.e., those too large to be safely sampled by wading) and,
depending on the scale and scope of programmatic data needs, can be used for
site-specific, watershed-wide, or other spatial scales. Also, while the protocols are
not intended for application to great rivers, adjustment of one or more components
of the protocols may make them suitable for those kinds of systems. The principal
purposes of this document are to:


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•	Serve as a scientific framework and foundation for bioassessment and
biocriteria, to be used by regulatory agencies to assist in the establishment
of WQS, designated use attainment, mitigation, restoration, TMDL needs
and responses and other needs;

•	Document and articulate the best science for sampling and assessing large,
nonwadeable rivers;

•	Provide the essential technical elements for routine biological monitoring
and assessment programs;

•	Foster clear communication among agencies and other entities for
mainstem rivers crossing jurisdictional boundaries; and

•	Describe how assessment procedures and strategies would be tailored for
different types of large rivers.

Results and	In September of 2005, the current draft version of the document was distributed to

Impact	6 individuals in the Office of Water (3 in the Office of Science and Technology

and 3 in the Office of Wetlands, Oceans, and Watersheds, the Regional Science
Liaison for each EPA region, and a multitude of EPA regional scientists. The
purpose of this initial distribution is to solicit comments on the "scope and
content" of the draft document prior to its' review outside the agency. Once
comments are received from OW and regional colleagues, a revised draft of the
document will be sent out for an extensive and thorough review. In addition to
EPA colleagues, this will include collaborators and experts external to the Agency.
When these reviews and comments have been received, a post-review draft will be
prepared for final approval prior to release. Final release for the document is
scheduled for October 2006.

Research Team: Joseph E. Flotemersch, Brent Johnson, Brad Autrey, and Karen
Blocksom, National Exposure Research Laboratory, U.S. Environmental
Protection Agency, 26 W. Martin Luther King Dr., Cincinnati, OH 45268 USA;
James (Sam) Stribling, Abby Markowitz, Michael Paul, JoAnne Lessard, Lei
Zheng, and Blaine Snyder, Tetra Tech, Inc., Owings Mills, MD, 21117 USA;
Kenneth S. Lubinski, U.S. Geological Survey, Upper Midwest Environmental
Sciences Center, La Crosse, WI 54603USA.

The current product is a draft report that is currently in wide review with EPA
program offices, Regional Science Liaisons, and regional scientists. Subsequent
to this process, the draft will be subjected to broad external review.

Future Research The final product of this research (scheduled for October 2006) will be an

integrated report that provides a technical framework for assessing biological
condition and a scientific basis for decision-making regarding large, non-wadeable
rivers.

Contacts for	Questions and inquiries can be directed to:

Additional	Joseph Flotemersch, Ph.D.

Information	US EPA

National Exposure Research Laboratory

Research
Collaboration and
Research
Products


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Cincinnati, Ohio 45268

Phone:513/569-7086

E-mail: flotemersch.joseph@epa.gov


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