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Unique	Chemistry

Regional

Moving	Science

U.S. Environmental Protection Agency, Office of Research and Development, National Exposure Research Laboratory,
Environmental Sciences Division, Las Vegas, Nevada

Introduction

Many of ORD's research projects relate to broad scientific
themes, such as biological and chemical indicators or computa-
tional toxicology. Others are discreet studies resulting from
requests by clients or contacts with collaborators (in a number of
scientific disciplines). This poster presents a montage of recent
"grass roots" research efforts that the Environmental Chemistry
Branch (ECB) at NERL-Las Vegas has conducted in response to
real-world analytical chemistry problems of the Regions, the
States, and Tribal authorities.

A Tale of Two Rivers

A six mile stretch of a
beautiful South Carolina
river became sick. State of
South Carolina officials
needed confirmation that
an organotin factory
might be responsible.
Water and fish are col-
lected. Using state-of-the art methodologies organotins
are detected in all samples. Data is provided to State of
South Carolina and Region 4.

Case goes to court - three company employees plead
guilty, they are sentenced, the factory is shut down and
fines are levied to help pay toward cleanup costs.

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What Do Dietary Surveys of Alaskan Tribes,
Inspection of Fish for NOAA, and Hair-Collection
in Washington State All Have in Common?
Mercury

Tribal authorities, the State of Alaska, and National
Institute of Environmental Health Sciences (NIEHS)
requested help for mercury dietary studies. Study
under way to look at mercury content in indigenous
food sources (fish, seagoing mammals) using a recently
developed method that can determine mercury directly
in solid matrices.

NOAA's
National Seafood
Inspection
Laboratory in Miss-
issippi requested
help to determine
mercury levels in
Gulf of Mexico fish.
Measurement-vali-

dation reports and
technical advice
were provided.

Collaborations
with Washington
State and Region
10 to assess the
mercury exposure
to an Asian-
American commu-
nity in the Puget Sound area. Hair-collection
and analysis protocols, study-design sugges-
tions, and data are being provided for risk
assessments.

Millions of tons of pesticides are used in the San
Joaquin Valley of California every year. How much is
transported by winds into the nearby southern Sierra
Nevada? Could this be a cause for the disappearance of
the mountain yellow-legged frog in much of the area?

ECB, working closely with EPA Biologists, is con-
ducting a comprehensive survey to help Region 9, the
National Park Service, and the State of California under-
stand the impact of pesticide contamination in the
alpine lakes of the southern Sierra Nevada mountains.
This data will better help our clients decide if steps need
to be taken to protect these sensitive ecosystems.

ICE-man Cometh

Region 2/State of New Jersey - Increased
incidence of childhood cancers at Toms River,
NJ. Help requested - ICE applied - identifica-
tions of unknown contaminants reported to
State of New Jersey - toxicological tests are now
under way.

Region 3 - Bad smelling water in nearby
town. A tar-like substance is removed from a
nearby site and sent to the laboratory - ICE
applied - identification made - tar-like sub-
stance linked to bad smelling water - source is
found for chemical contaminants.

Region 4 - Well water samples from nearby
town are contaminated with unknown contami-
nants - increasing illness - ICE applied - identi-
fications made - risk assessments under way.

Region 9 - Super fund site needs cleanup -
ICE applied - identifications made - Potentially
Responsible Parties (PRPs) can be traced and
levied to pay for cleanups.

Ion Composition Elucidation - ICE

ICE is a newly developed high resolution mass spec-
trometric software technique that often allows the identifi-
cation of unknown chemical
contaminants under non-
ideal circumstances.

http://zmvw.epa.gov/herlesdl/che mistry/ice/about. htm

This Isn't Your Mother's Henny
Penny Fairy Tale

Region 3 - Delmarva Peninsula -



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than people. Many dietary arsenical
feed additives are fed to chickens to
enhance growth. Runoff from
V 1 farm waste ponds and

\ Jen manure leads to
t . _ \ jstream contamina-

with USGS to assist
^/Region 3 evaluate risk

runoff.

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What Are You
Drinking in Your Milk?

Region 2 requests assistance in identifying whether vinyl chloride
(known human carcinogen) is present in milk samples. A ECB-unique
patented process (vacuum distillation) is applied to detecting volatile
organics. Leads to wider survey of milk from Nevada,
Arizona, Utah, and California for the fuel additive MTBE
and other volatile organics.

Protecting Your Environment
With Environmental Forensic
Chemistry

ECB expertise: mass spectrometry, computational toxicology, sepa-
ration techniques, sample preparation and cleanup techniques,
ground-water migration, ICE, high resolution mass spectrometry, mer-
cury methodologies, inorganic methodologies, organic methodologies,
vacuum distillation, volatile organic methodologies, mass spectrome-
try, organotin speciation, PPCPs,^ arsenic speciation, gas chromatogra-
phy/ mass spectrometry, unique MS sample introduction techniques,
mass spectral interpretation.

^http://zvww.epa.gov/nerlesdl/chemistry/pharma/

Notice

The U.S. Environmental Protection Agency (EPA), through its Office of Research and
Development (ORD), funded this research and approved this poster presentation. The actual
presentation has been peer reviewed by EPA

193ECB03DB.Jones-Lepp

PRO'



-Partnering to Protect Human Health and the Environment


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