Final Report on Acute and Chronic Toxicity of Nitrate,
Nitrite, Boron, Manganese, Fluoride, Chloride and
Sulfate to Several Aquatic Animal Species
Prepared for:
United States Environmental Protection Agency
Office of Science and Technology
Health and Ecological Criteria Division
EPA Contract: EP-C-09-001
Work Assignments: B-12 and 1-12
Contacts: Tom Poleck and Brian Thompson
Prepared by:
GleC
Great Lakes Environmental Center
739 Hastings Street
Traverse City, Michigan 49686
Phone: (231)941-2230
Facsimile: (231)941-2240
Contacts: Jamie Saxton and Mailee Garton
SUBMISSION DATE: October 22, 2010
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FORWARD
The U.S. Environmental Protection Agency is charged under the Clean Water Act
(§304(a)) with periodically updating water quality criteria (WQC) to protect existing and
designated uses such as aquatic life and human health. Two States in EPA Region 5
identified the need to develop criteria for which no national guidance has been developed
to date. To support these State efforts, EPA Region 5 contracted (Contract EP-C-09-001,
Work Assignments B-12 and 1-12) with Great Lakes Environmental Center (GLEC) and
its subcontractor, the Illinois Natural History Survey (INHS), to test the acute and chronic
toxic effects of nitrate, nitrite, boron, manganese, fluoride, chloride and sulfate on several
aquatic animal species. The results of this Work Assignment are contained in this report.
Any comments on this document should be sent to the Work Assignment Manager,
Thomas Poleck at poleck.thomas@epa.gov or
U.S. EPA Region 5
Water Quality Branch (WQ-16J)
77 W. Jackson Blvd.
Chicago, IL 60604
NOTICE: This document has been reviewed in accordance with U.S. EPA policy. While
every effort was made to ensure the validity of the data in this report, any State, Tribe or
other party should verify for themselves that the data meets all appropriate data quality
requirements. For example, rearing and testing methods for Hyalella have not been
standardized and therefore must be evaluated as to appropriateness in any dataset used for
determining WQC. Mention of trade names or commercial products does not constitute
endorsement or recommendation for use.
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TABLE OF CONTENTS
Executive Summary viii
Introduction 1
Materials and Methods 4
Chemicals Tested and Toxicology Tests Performed 10
Nitrate 10
96-hr Toxicity of Nitrate on Lampsilis siliquoidea11
96-hr Toxicity of Nitrate on Megalonaias nervosa 15
48-hr Toxicity of Nitrate on Chironomus dilutus19
96-hr Toxicity of Nitrate on Amphinemura delosa 23
96-hr Toxicity of Nitrate on Sphaerium simile 27
96-hr Toxicity of Nitrate on Hyalella azteca 31
96-hr Toxicity of Nitrate on Pimephales promelas3 5
32-day Toxicity of Nitrate on Pimephales promelas 3 9
Nitrite 44
96-hr Toxicity of Nitrite on Lampsilis siliquoidea 45
48-hr Toxicity of Nitrite on Chironomus dilutus 49
96-hr Toxicity of Nitrite on Amphinemura delosa 5 3
96-hr Toxicity of Nitrite on Sphaerium simile 5 7
96-hr Toxicity of Nitrite on Hyalella azteca_61
96-hr Toxicity of Nitrite on Pimephales promelas 65
32-day Toxicity of Nitrite on Pimephales promelas 69
48-hr Toxicity of Nitrite on Ceriodaphnia dubia75
7-day Toxicity of Nitrite on Ceriodaphnia dubia 79
Boron 83
96-hr Toxicity of Boron on Lampsilis siliquoidea 84
96-hr Toxicity of Boron on Megalonaias nervosa 88
96-hr Toxicity of Boron on Ligumia recta 92
96-hr Toxicity of Boron on Pimephales promelas 96
32-day Toxicity of Boron on Pimephales promelas 100
48-hr Toxicity of Boron (pH 7.75) on Ceriodaphnia dubia 106
96-hr Toxicity of Boron (pH 6.75) on Pimephales promelas 110
96-hr Toxicity of Boron (pH 7.75) on Pimephales promelas 115
96-hr Toxicity of Boron (pH 8.75) on Pimephales promelas 120
Manganese 125
96-hr Toxicity of Manganese on Lampsilis siliquoidea 126
96-hr Toxicity of Manganese on Megalonaias nervosa 13 0
Fluoride .134
96-hr Toxicity of Fluoride on Sphaerium simile 13 5
96-hr Toxicity of Fluoride on Hyalella azteca 13 9
Chloride .143
96-hr Toxicity of Chloride on Musculium transversum 144
Sulfate 148
96-hr Toxicity of Sulfate on Ligumia recta 149
96-hr Toxicity of Sulfate on Megalonaias nervosa 153
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TABLE OF CONTENTS (CONT'D)
References 157
Appendi
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Appendix
1: Raw Data for 96-hr Toxicity of Nitrate on Lampsilis siliquoidea
2: Raw Data for 96-hr Toxicity of Nitrate on Megalonaias nervosa
3: Raw Data for 48-hr Toxicity of Nitrate on Chironomus dilutus
4: Raw Data for 96-hr Toxicity of Nitrate on Amphinemura delosa
5: Raw Data for 96-hr Toxicity of Nitrate on Sphaerium simile
6: Raw Data for 96-hr Toxicity of Nitrate on Hyalella azteca
7: Raw Data for 96-hr Toxicity of Nitrate on Pimephales promelas
8: Raw Data for 32-day Toxicity of Nitrate on Pimephales promelas
9: Raw Data for 96-hr Toxicity of Nitrite on Lampsilis siliquoidea
10: Raw Data for 48-hr Toxicity of Nitrite on Chironomus dilutus
11: Raw Data for 96-hr Toxicity of Nitrite on Amphinemura delosa
12: Raw Data for 96-hr Toxicity of Nitrite on Sphaerium simile
13: Raw Data for 96-hr Toxicity of Nitrite on Hyalella azteca
14: Raw Data for 96-hr Toxicity of Nitrite on Pimephales promelas
15: Raw Data for 32-day Toxicity of Nitrite on Pimephales promelas
16: Raw Data for 48-hr Toxicity of Nitrite on Ceriodaphnia dubia
17: Raw Data for 7-day Toxicity of Nitrite on Ceriodaphnia dubia
18: Raw Data for 96-hr Toxicity of Boron on Lampsilis siliquoidea
19: Raw Data for 96-hr Toxicity of Boron on Megalonaias nervosa
20: Raw Data for 96-hr Toxicity of Boron on Ligumia recta
21: Raw Data for 96-hr Toxicity of Boron on Pimephales promelas
22: Raw Data for 32-day Toxicity of Boron on Pimephales promelas
23: Raw Data for 48-hr Toxicity of Boron (pH 7.75) on Ceriodaphnia dubia
24: Raw Data for 96-hr Toxicity of Boron (pH 6.75) on Pimephales promelas
25: Raw Data for 96-hr Toxicity of Boron (pH 7.75) on Pimephales promelas
26: Raw Data for 96-hr Toxicity of Boron (pH 8.75) on Pimephales promelas
27: Raw Data for 96-hr Toxicity of Manganese on Lampsilis siliquoidea
28: Raw Data for 96-hr Toxicity of Manganese on Megalonaias nervosa
29: Raw Data for 96-hr Toxicity of Fluoride on Sphaerium simile
30: Raw Data for 96-hr Toxicity of Fluoride on Hyalella azteca
31: Raw Data for 96-hr Toxicity of Chloride on Musculium transversum
32: Raw Data for 96-hr Toxicity of Sulfate on Ligumia recta
33: Raw Data for 96-hr Toxicity of Sulfate on Megalonaias nervosa
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LIST OF TABLES
Table
1.
Table
2.
Table
3.
Table
4.
Table
5.
Table
6.
Table
7.
Table
8.
Table
9.
Table
10
Table
11
Table
12
Table
13
Table
14
Table
15
Table
16
Table
17
Table
18
Table
19
Table
20
Table
21
Table
22
Table
23
Table
24
Table
25
Table
26
Table
27
Table
28
Experimental matrix for toxicity testing completed by GLEC and INHS.
ASTM standard used for each toxicity test completed by GLEC and INHS.
Chemical properties of de-chlorinated Lake Michigan (city of Traverse
City) water.
Analytical chemistry methods for each toxicity test completed by GLEC
and INHS.
LC50 estimates for toxicity tests performed using nitrate.
Test conditions for 96-hour toxicity test on Lampsilis siliquoidea with
nitrate.
Test results for 96-hour toxicity test on Lampsilis siliquoidea with nitrate.
Analytical chemistry data for 96-hour toxicity test on Lampsilis
siliquoidea with nitrate.
Test conditions for 96-hour toxicity test on Megalonaias nervosa with
nitrate.
Test results for 96-hour toxicity test on Megalonaias nervosa with nitrate.
Analytical chemistry data for 96-hour toxicity test on Megalonaias
nervosa with nitrate.
Test conditions for 48-hour toxicity test on Chironomus dilutus with
nitrate.
Test results for 48-hour toxicity test on Chironomus dilutus with nitrate.
Analytical chemistry data for 48-hour toxicity test on Chironomus dilutus
with nitrate.
Test conditions for 96-hour toxicity test on Amphinemura delosa with
nitrate.
Test results for 96-hour toxicity test on Amphinemura delosa with nitrate.
Analytical chemistry data for 96-hour toxicity test on Amphinemura
delosa with nitrate.
Test conditions for 96-hour toxicity test on Sphaerium simile with nitrate.
Test results for 96-hour toxicity test on Sphaerium simile with nitrate.
Analytical chemistry data for 96-hour toxicity test on Sphaerium simile
with nitrate.
Test conditions for 96-hour toxicity test on Hyalella azteca with nitrate.
Test results for 96-hour toxicity test on Hyalella azteca with nitrate.
Analytical chemistry data for 96-hour toxicity test on Hyalella azteca with
nitrate.
Test conditions for 96-hour toxicity test on Pimephalespromelas with
nitrate.
Test results for 96-hour toxicity test on Pimephales promelas with nitrate.
Analytical chemistry data for 96-hour toxicity test on Pimephales
promelas with nitrate.
Test conditions for 32-day toxicity test on Pimephales promelas with
nitrate.
Test results for 32-day toxicity test on Pimephales promelas with nitrate.
111
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LIST OF TABLES (CONT'D)
Table
29.
Survival and growth data for 32-day toxicity test on Pimephales promelas
with nitrate.
Table
30.
Analytical chemistry data for 32-day toxicity test on Pimephales promelas
with nitrate.
Table
31.
LC50 estimates for toxicity tests performed using nitrite.Table 55. Test
conditions for 48-hour toxicity test on Ceriodaphnia dubia with nitrite.
Table
32.
Test conditions for 96-hour toxicity test on Lampsilis siliquoidea with
nitrite.
Table
33.
Test results for 96-hour toxicity test on Lampsilis siliquoidea with nitrite.
Table
34.
Analytical chemistry data for 96-hour toxicity test on Lanpsilis siliquoidea
with nitrite.
Table
35.
Test conditions for 48-hour toxicity test on Chironomus dilutus with
nitrite.
Table
36.
Test results for 48-hour toxicity test on Chironomus dilutus with nitrite.
Table
37.
Analytical chemistry data for 48-hour toxicity test on Chironomus dilutus
with nitrite.
Table
38.
Test conditions for 96-hour toxicity test on Amphinemura delosa with
nitrite.
Table
39.
Test results for 96-hour toxicity test on Amphinemura delosa with nitrite.
Table
40.
Analytical chemistry data for 96-hour toxicity test on Amphinemura
delosa with nitrite.
Table
41.
Test conditions for 96-hour toxicity test on Sphaerium simile with nitrite.
Table
42.
Test results for 96-hour toxicity test on Sphaerium simile with nitrite.
Table
43.
Analytical chemistry data for 96-hour toxicity test on Sphaerium simile
with nitrite.
Table
44.
Test conditions for 96-hour toxicity test on Hyalella azteca with nitrite.
Table
45.
Test results for 96-hour toxicity test on Hyalella azteca with nitrite.
Table
46.
Analytical chemistry data for 96-hour toxicity test on Hyalella azteca with
nitrite.
Table
47.
Test conditions for 96-hour toxicity test on Pimephales promelas with
nitrite.
Table
48.
Test results for 96-hour toxicity test on Pimephales promelas with nitrite.
Table
49.
Analytical chemistry data for 96-hour toxicity test on Pimephales
promelas with nitrite.
Table
50.
Test conditions for 32-day toxicity test on Pimephales promelas with
nitrite.
Table
51.
Test results for 32-day toxicity test on Pimephales promelas with nitrite.
Table
52.
Survival and growth data for 32-day toxicity test on Pimephales promelas
with nitrite.
Table
53.
Analytical chemistry data for 32-day toxicity test on Pimephales promelas
with nitrite.
Table
54.
Test conditions for 48-hour toxicity test on Ceriodaphnia dubia with
nitrite.
Table
55.
Test results for 48-hour toxicity test on Ceriodaphnia dubia with nitrite.
iv
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LIST OF TABLES (CONT'D)
Table
56.
Analytical chemistry data for 48-hour toxicity test on Ceriodaphnia dubia
with nitrite.
Table
57.
Test conditions for 7-day toxicity test on Ceriodaphnia dubia with nitrite.
Table
58.
Test results for 7-day toxicity test on Ceriodaphnia dubia with nitrite.
Table
59.
Analytical chemistry data for 7-day toxicity test on Ceriodaphnia dubia
with nitrite.
Table
60.
LC50 estimates for toxicity tests performed using boron.
Table
61.
Test conditions for 96-hour toxicity test on Lampsilis siliquoidea with
boron.
Table
62.
Test results for 96-hour toxicity test on Lampsilis siliquoidea with boron.
Table
63.
Analytical chemistry data for 96-hour toxicity test on Lampsilis
siliquoidea with boron.
Table
64.
Test conditions for 96-hour toxicity test on Megalonaias nervosa with
boron.
Table
65.
Test results for 96-hour toxicity test on Megalonaias nervosa with boron.
Table
66.
Analytical chemistry data for 96-hour toxicity test on Megalonaias
nervosa with boron.
Table
67.
Test conditions for 96-hour toxicity test on Ligumia recta with boron.
Table
68.
Test results for 96-hour toxicity test on Ligumia recta with boron.
Table
69.
Analytical chemistry data for 96-hour toxicity test on Ligumia recta with
boron.
Table
70.
Test conditions for 96-hour toxicity test on Pimephalespromelas with
boron.
Table
71.
Test results for 96-hour toxicity test on Pimepehalespromelas with boron.
Table
72.
Analytical chemistry data for 96-hour toxicity test on Pimephales
promelas with boron.
Table
73.
Test conditions for 32-day toxicity test on Pimephales promelas with
boron.
Table
74.
Test results for 32-day toxicity test on Pimepehales promelas with boron.
Table
75.
Survival and growth data for 32-day toxicity test on Pimephales promelas
with boron.
Table
76.
Analytical chemistry data for 32-day toxicity test on Pimephales promelas
with boron.
Table
77.
Test conditions for 48-hour toxicity test on Ceriodaphnia dubia with
boron (pH 7.75).
Table
78.
Test results for 48-hour toxicity test on Ceriodaphnia dubia with boron at
pH 7.75.
Table
79.
Analytical chemistry data for 48-hour toxicity test on Ceriodaphnia dubia
with boron at pH 7.75.
Table
80.
Test conditions for 96-hour toxicity test on Pimephales promelas with
boron (pH 6.75).
Table
81.
Test results for 96-hour toxicity test on Pimephales promelas with boron
at pH 6.75.
v
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LIST OF TABLES (CONT'D)
Table
82.
Table
83.
Table
84.
Table
85.
Table
86.
Table
87.
Table
88.
Table
89.
Table
90.
Table
91.
Table
92.
Table
93.
Table
94.
Table
95.
Table
96.
Table
97.
Table
98.
Table
99.
Table
100
Table
101
Table
102
Table
103
Table
104
Table
105
Table
106
Analytical chemistry data for 96-hour toxicity test on Pimephales
promelas with boron at pH 6.75.
Test conditions for 96-hour toxicity test on Pimephales promelas with
boron (pH 7.75).
Test results for 96-hour toxicity test on Pimephales promelas with boron
at pH 7.75.
Analytical chemistry data for 96-hour toxicity test on Pimephales
promelas with boron at pH 7.75.
Test conditions for 96-hour toxicity test on Pimephales promelas with
boron (pH 8.75).
Test results for 96-hour toxicity test on Pimephales promelas with boron
at pH 8.75.
Analytical chemistry data for 96-hour toxicity test on Pimephales
promelas with boron at pH 8.75.
LC50 estimates for toxicity tests performed using manganese.
Test conditions for 96-hour toxicity test on Lampsilis siliquoidea with
manganese.
Test results for 96-hour toxicity test on Lampsilis siliquoidea with
manganese.
Analytical chemistry data for 96-hour toxicity test on Lampsilis
siliquoidea with manganese.
Test conditions for 96-hour toxicity test on Megalonaias nervosa with
manganese.
Test results for 96-hour toxicity test on Megalonaias nervosa with
manganese.
Analytical chemistry data for 96-hour toxicity test on Megalonaias
nervosa with manganese.
LC50 estimates for toxicity tests performed using fluoride.
Test conditions for 96-hour toxicity test on Sphaerium simile with
fluoride.
Test results for 96-hour toxicity test on Sphaerium simile with fluoride.
Analytical chemistry data for 96-hour toxicity test Sphaerium simile with
fluoride.
Test conditions for 96-hour toxicity test on Hyalella azteca with fluoride.
Test results for 96-hour toxicity test on Hyalella azteca with fluoride.
Analytical chemistry data for 96-hour toxicity test on Hyalella azteca with
fluoride.
LC50 estimate for toxicity test performed using chloride.
Test conditions for 96-hour toxicity test on Musculium transversum with
chloride.
Test results for 96-hour toxicity test on Musculium transversum with
chloride.
Analytical chemistry data for 96-hour toxicity test on Musculium
transversum with chloride.
VI
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LIST OF TABLES (CONT'D)
Table
107
Table
108
Table
109
Table
110
Table
111
Table
112
Table
113
LC50 estimates for toxicity tests performed using sulfate.
Test conditions for 96-hour toxicity test on Ligumia recta with sulfate.
Test results for 96-hour toxicity test on Ligumia recta with sulfate.
Analytical chemistry data for 96-hour toxicity test on Ligumia recta with
sulfate.
Test conditions for 96-hour toxicity test on Ligumia recta with sulfate.
Test results for 96-hour toxicity test on Megalonaias nervosa with sulfate.
Analytical chemistry data for 96-hour toxicity test on Megalonaias
nervosa with sulfate.
vii
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EXECUTIVE SUMMARY
EPA guidance (EPA 1985) requires toxicity data from eight taxonomic groups, at a
minimum, to derive a fully-supported water quality criterion. Two States in Region 5 are
in the process of developing criteria for nitrate, boron, manganese and fluoride. This
project was designed to fill existing data gaps by conducting toxicity testing on additional
aquatic species for which data are absent from the literature. In addition, EPA Region 5
included several tests on nitrite, chloride and sulfate to meet other data needs and to
support ongoing consultations with the U.S. Fish and Wildlife Service under the
Endangered Species Act.
Specifically, the toxicity tests that were conducted are listed below along with their
resultant lethal and effective concentrations.
A. Nitrate
1. Lampsilis siliquoidea (96-hour acute test)
[LC50 = 3 5 7 mg/L]
2. Megalonaias nervosa (96-hour acute test)
[LC50 = 93 7 mg/L]
3. Chironomus dilutus (48-hour acute test)
[LC50 = 278 mg/L]
4. Amphinemura delosa (96-hour acute test)
[LC50 = 45 6 mg/L]
5. Sphaerium simile (96-hours acute test)
[LC50 = 371 mg/L]
6. Hyalella azteca (96-hour acute test)
[LC50 = 16.4 mg/L]
7. Pimephales promelas (96-hour acute test)
[LC50 = 415 mg/L]
8. Pimephales promelas (32-day chronic test)
[Survival LC50 = 76.8 mg/L, LC25 = 68.2 mg/L, LC2o = 64.6 mg/L,
LC10 = 55.5 mg/L, NOEC = 49 mg/L, LOEC = 109 mg/L]
[Growth EC50 = 91.3 mg/L, EC25 = 65.3 mg/L, EC2o = 59.8 mg/L,
EC10 = 46.7 mg/L, NOEC = 49 mg/L, LOEC = 109 mg/L]
B. Nitrite
9.
Lampsilis siliquoidea (96-hour acute test)
[LC50 = 177 mg/L]
10.
Chironomus dilutus (48-hour acute test)
[LC50= 15.6 mg/L]
11.
Amphinemura delosa (96-hour acute test)
[LC50 =1.0 mg/L]
12.
Sphaerium simile (96-hours acute test)
[LC50 = 55.7 mg/L]
13.
Hyalella azteca (96-hour acute test)
[LC50 =1.5 mg/L]
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14. Pimephalespromelas (96-hour acute test)
[LC50 = 27.0 mg/L]
15. Pimephales promelas (32-day chronic test)
[Survival LC50 = 11.3 mg/L, LC25 = 7.0 mg/L, LC2o = 5.7 mg/L,
LCio = 2.3 mg/L, NOEC = 6.8 mg/L, LOEC = 15.0 mg/L]
[Growth EC50 = 14.6 mg/L, EC25 = 11.7 mg/L, EC2o = 11.0 mg/L,
ECio = 9.3 mg/L, NOEC = 6.8 mg/L, LOEC = 15.0 mg/L]
16. Ceriodaphnia dubia (48-hour acute test)
[LC5o = 4.6 mg/L]
17. Ceriodaphnia dubia (7-day chronic test)
[Survival LC50 = 4.7 mg/L, LC25 = 3.5 mg/L, LC2o = 3.3 mg/L,
LC10 = 2.5 mg/L, NOEC = 3.6 mg/L, LOEC = 7.3 mg/L]
[Reproduction EC50 = 2.4 mg/L, EC25 = 1.9 mg/L, EC2o = 1.7
mg/L, EC10 = 1.4 mg/L, NOEC = 0.82 mg/L, LOEC =1.8 mg/L]
C. Boron
18. Lampsilis siliquoidea (96-hour acute test)
[LC50 = 137 mg/L]
19. Megalonaias nervosa (96-hour acute test)
[LC50 = >544 mg/L]
20. Ligumia recta (96-hour acute test)
[LC50 = 147 mg/L]
21. Pimephales promelas (96-hour acute test)
[LC50= 101 mg/L]
22. Pimephales promelas (32-day chronic test)
[Survival LC50 = 28.4 mg/L, LC25 = 20.8 mg/L, LC20 = 18.6 mg/L,
LC10 = 12.8 mg/L, NOEC = 12.9 mg/L, LOEC = 27.4 mg/L]
[Growth EC50 = 28.7 mg/L, EC25 = 25.1 mg/L, EC20 = 24.2 mg/L,
EC10 = 21.9 mg/L, NOEC = <5.9 mg/L, LOEC = 5.9 mg/L]
23. Ceriodaphnia dubia (48-hour acute test at pH 7.75)
[LC50 = 76.9 mg/L]
24. Pimephales promelas (96-hour acute test at pH 6.75)
[LC50 = 70.6 mg/L]
25. Pimephales promelas (96-hour acute test at pH 7.75)
[LC50 = 137 mg/L]
26. Pimephales promelas (96-hour acute test at pH 8.75 )
[LC50 = 133 mg/L]
D. Manganese
27. Lampsilis siliquoidea (96-hour acute test)
[LC50 = 43.3 mg/L]
28. Megalonaias nervosa (96-hour acute test)
[LC50 = 31.5 mg/L]
IX
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E. Fluoride
29. Sphaerium simile (96-hours acute test)
[LC50 = 62.0 mg/L]
30. Hyalella azteca (96-hour acute test)
[LC50= 13.4 mg/L]
F. Chloride
31. Musculium transversum (96-hours acute test)
[LC50 = 1,93 0 mg/L]
G. Sulfate
32. Ligumia recta (96-hour acute test)
[LC50 = 1,483 mg/L]
33. Megalonaias nervosa (96-hour acute test)
[LC50 = 3,3 78 mg/L]
x
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INTRODUCTION
There are no federal water quality criteria for the protection of aquatic life for nitrate,
nitrite, boron, manganese and fluoride. States in Region 5 of the United States
Environmental Protection Agency (EPA), particularly the states of Minnesota and
Illinois, are in the process of revising or developing water quality criteria for each of
these parameters, with the exception of nitrite. In addition, EPA recently completed a
review of the available data and undertook a draft revision of the chloride criterion.
Nitrate and Nitrite
Biological field data generated by the State of Minnesota, as well as laboratory-derived
toxicity test data, suggest that nitrate at concentrations similar to those observed in the
surface waters of Minnesota may cause adverse effects to aquatic life. Much of the nitrate
toxicity literature focuses on the effects of nitrate, especially the effects of wastes
associated with fish cultures. More recent literature reports have documented that
amphibians and invertebrates appear to be more sensitive to nitrate than fish.
The state of Minnesota is evaluating the need to develop a nitrate criterion to protect
aquatic life as part of its current triennial Water Quality Standards (WQS) review. Efforts
by Minnesota to develop aquatic life criteria for nitrate have involved a review of all the
pertinent literature to date. The primary sources were the ECOTOX database, as well as a
comprehensive search of the open literature. The scientific studies were each evaluated
based on methodologies in the 1985 guidance of EPA (EPA 1985), and produced two
observations: 1. there is a wide variation in response among aquatic organisms to nitrate
toxicity; and 2. the research has documented the toxicity of nitrate for some aquatic
organisms at environmentally relevant concentrations. In addition, the literature review
found seven acceptable studies that reported acute toxicity values for eleven different
species. Invertebrates (especially aquatic insects) were among the most sensitive taxa.
Associated chronic values from these studies were found to be acceptable for three
separate species, one each for a fish, an amphibian and a cladoceran. Unfortunately, these
data are insufficient to develop Tier I aquatic life criteria for nitrate. Acceptable toxicity
data are lacking for two required taxonomic categories: a benthic invertebrate and one
other invertebrate.
Additional nitrite acute toxicity tests are also required to complete the data set for
calculating a water quality criterion consistent with EPA's 1985 guidelines. Acute tests
with an amphipod (e.g., Hyalella azteca) and a chironomid (e.g., Chironomus dilutus)
would best serve this purpose. In addition, completing acute and chronic (early life stage)
tests with the fathead minnow (Pimephalespromelas) would augment existing data for
fish, and add an additional acute to chronic ratio for calculating a chronic criterion.
Within Region 5, field data have shown elevated levels of nitrate. Given the relationship
between nitrate and nitrite, the potential for nitrite to exist in the aquatic environment
under certain circumstances, the toxic effects of nitrite, and the lack of nitrite data for
1
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most organisms other than fish, EPA wanted to assess the potential need for a nitrite
criterion by generating additional nitrite toxicity data for several species of organisms.
Boron, Manganese and Fluoride
The state of Illinois is in the process of revising its aquatic life criteria for boron,
manganese and fluoride. The Illinois EPA conducted a literature review and identified the
need for acute and chronic (early life stage) tests with P. promelas to add an additional
acute to chronic ratio for calculating a chronic criterion for boron. Additionally, recent
toxicity data suggest that pH affects the toxicity of boron, which is consistent with the
fact that borate exists in water as a pH-dependent mixture of various borate ions.
Therefore, EPA wanted to assess the effects of pH on the toxicity of boron on
Ceriodaphnia dubia and P. promelas.
For fluoride, the data needs include acute and chronic tests on Hyalella, in order to
develop an additional acute to chronic ratio with an organism other than Pimephales or
Daphnia. The available acute data for an amphipod also need to be supplemented with
tests conducted using a range of test water hardness levels. In order to facilitate the
review of these criteria, and to provide additional support for the database, EPA also
wanted to generate freshwater mussel toxicity data.
Chloride
EPA recently reviewed and developed additional toxicity data for chloride. As part of the
review of that data and subsequent development of a draft revision of the criteria, EPA
determined that additional toxicity information on resident fingernail clams (other than
those from the genus Sphaerium) is desirable. To facilitate the acquisition of these data,
EPA is interested in additional toxicity tests on other resident fingernail clams, such as
Musculium sp.
Sulfate
Additional sulfate toxicity data are required to enhance EPA's basis for making a
determination under the Endangered Species Act (ESA) that the recently adopted sulfate
criteria by Illinois and Indiana are not likely to adversely affect threatened and
endangered species. EPA Region 5 is currently consulting under Section 7 of the ESA on
the Agency's approval of the Illinois and Indiana surface water quality standards. Data on
the sensitivity of mussels are limited and mussels are often one of the most sensitive
species to adverse water quality conditions. Therefore, EPA wanted to generate sulfate
toxicity data for two mussel species (Ligumia recta and Megalonaias nervosa).
Because of the data needs outlined above, Great Lakes Environmental Center (GLEC)
and its subcontractor the Illinois Natural History Survey (INHS) were contracted by the
EPA to augment existing toxicity data through a series of toxicity tests. This document
presents in detail the materials and methods by which GLEC and INHS performed these
2
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toxicity tests with nitrate, nitrite, boron, manganese, fluoride, sulfate and chloride. Also
included are the results of the toxicity tests, including % survival and mortality, growth
and reproduction (where appropriate), water chemistry data, statistical analyses
performed on the data and toxicology endpoint estimates.
3
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MATERIALS AND METHODS
GLEC and INHS performed a total of 33 acute and chronic toxicity tests (Table 1). Tests
on C. dubia, C. dilutus, H. azteca and P. promelas were completed by GLEC; tests on all
other species were completed by INHS. Eight of these tests were designed to determine
acute and/or chronic toxicity of the chemical nitrate to seven different species of aquatic
organisms; nine additional tests were designed to determine acute and/or chronic toxicity
of the chemical nitrite to seven different species of aquatic organisms. Five different
species of aquatic organisms were exposed to boron under acute and/or chronic
conditions during nine separate toxicity tests while two aquatic organisms were exposed
to manganese under acute conditions. Acute toxicity was determined for fluoride,
chloride and sulfate using one of four species of mussels and H. azteca.
Toxicity testing under this Work Assignment was accomplished using the methods
outlined in the ASTM standards (Table 2), GLEC and INHS standard operating
procedures (GLEC 2009 a, GLEC 2009 b, GLEC 2009 c, GLEC 2009 d, GLEC 2009 e,
GLEC 2009 f, GLEC 2009 g and INHS 2009) and the Quality Assurance Project Plan
(GLEC 2009) developed for this Work Assignment. Prior to completing the toxicity test
for each test chemical/organism combination, range-finding toxicity tests were initiated
to determine the appropriate test chemical concentrations that would "bracket" the LC50
estimates. The range of concentrations for these tests was determined either from the
literature or from laboratory experiences with similar organisms. Once the appropriate
range in test chemical concentrations was defined for each test chemical/organism
combination, definitive toxicity testing was completed.
Dilution water used for the tests consisted of either EPA Moderately Hard Reconstituted
Water (MHRW) (EPA 2002a and EPA 2002 b), EPA Dilute Mineral Water (DMW)
(EPA 2002 a and EPA 2002 b) or de-chlorinated Lake Michigan water (City of Traverse
City, Michigan water passed through an activated carbon filter). The chemical properties
of the de-chlorinated Lake Michigan water are presented in Table 3; these data were
generated both before and after the P. promelas flow-through test with boron at pH 8.75.
Water quality parameters (water temperature, pH, dissolved oxygen (DO), conductivity,
hardness, alkalinity and ammonia) were measured throughout the tests at pre-determined,
test-specific intervals. Water temperature, pH, DO and conductivity were measured in
situ using calibrated meters. Hardness, alkalinity and ammonia were measured in water
collected from test chambers using methods outlined in SM 2340 C (APHA et al. 2006
a), SM 2320 B (APHA et al. 2006 b) and SM 4500-NH3 (APHA et al. 2006 c),
respectively. Test concentrations of the target test chemicals were also verified using the
analytical chemistry methods outlined in Table 4 and laboratory-specific standard
operating procedures (GLEC 2008, GLEC 2009 h, Simmerman 2008, UL 2008 a, UL
2008 b, UL 2009 a and UL 2009 b). For toxicity testing completed by GLEC, target test
chemicals were verified by the GLEC analytical chemistry laboratory (nitrate and nitrite)
or SOS Analytical (boron and fluoride). Target test chemical sample analysis for toxicity
testing completed by INHS was completed by the INHS analytical laboratory (nitrate and
nitrite) or Underwriters Laboratories (boron, fluoride, chloride, sulfate and manganese).
4
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Once the toxicity tests were complete, statistical analysis of the mortality (and where
appropriate in chronic tests, growth or reproduction) data was completed following
guidance outlined in EPA (2002 a), EPA (2002 b) and the ASTM methods (Table 2).
Specifically, data were analyzed using either TOXSTAT software (Gulley 1996) or
EPA's Toxicity Relationship Analysis Program (TRAP, Version 1.00). Where
appropriate, data were transformed to meet the assumptions of normality and
homogeneity of variance. Analysis of variance (ANOVA), parametric (e.g. Probit) or
non-parametric (e.g. Spearman-Karber) tests and mathematical models (TRAP) were then
used to determine appropriate toxicology endpoints, including: LCso.LCso, LC25,LC2o
and LCio estimates; EC50, EC2s. EC2o, ECio estimates for growth or reproduction; and
NOEC and LOEC estimates.
5
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Table 1. Experimental matrix for toxicity testing completed by GLEC and INHS.
Test Species and Duration
Xilrale
Nitrite
Boron
Manganese
Ceriodaphnia dubia - 48 hr
X
Lampsilis siliquoidea 96 hr
X
X
X
X
Chironomus dilutus - 48 hr
X
X
Amphinemura delosa - 96 hr
X
X
Sphaerium simile - 96 hr
X
X
Hyalella azteca - 96 hr
X
X
Pimephales promelas - 96 hr
X
X
X
Pimephalespromelas - 32 day
X
X
X
Ceriodaphnia dubia - 7 day
X
Ceriodaphnia dubia - 48 hr (pH 7.75)
X
Pimephales promelas - 96 hr (pH 6.75)
X
Pimephales promelas - 96 hr (pH 7.75)
X
Pimephales promelas - 96 hr (pH 8.75)
X
Ligumia recta - 96 hr
X
Megalonaias nervosa - 96 hr
X
X
X
Test Species and Duration
Fluoride
Chloride
Sulfate
Sphaerium simile - 96 hr
X
Hyalella azteca - 96 hr
X
Musculium transversum - 96 hr
X
Ligumia recta - 96 hr
X
Megalonaias nervosa - 96 hr
X
6
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Table 2. ASTM standard used for each toxicity test completed by GLEC and INHS.
Test Species and Duration
Nitrate
Nitrite
Boron
Ceriodaphnia dubia - 48 hr
ASTM E729
Lampsilis siliquoidea 96 hr
ASTM 2455-06
ASTM 2455-06
ASTM 2455-1 ^
Chironomus dilutus - 48 hr
ASTM E729
ASTM E729
Amphinemura delosa - 96 hr
ASTM E729
ASTM E729
Sphaerium simile - 96 hr
ASTM E729
ASTM E729
Hyalella azteca - 96 hr
ASTM E729
ASTM E729
Pimephales promelas - 96 hr
ASTM E729
ASTM E729
ASTM E729
Pimephales promelas - 32 day
ASTM El241
ASTM E1241
ASTM E1241
Ceriodaphnia dubia -1 day
ASTM El295
Ceriodaphnia dubia - 48 hr (pH 7.75)
ASTM l!7:w
Pimephales promelas - 96 hr (pH 6.75)
ASTM l!7:w
Pimephales promelas - 96 hr (pH 7.75)
ASTM l!7:w
Pimephales promelas - 96 hr (pH 8.75)
ASTM l!7:w
Ligumia recta - 96 hr
ASTM 2455-iift
Megalonaias nervosa - 96 hr
ASTM 2455-06
ASTM 2455-06
Test Species and Duration
Manganese
Fluoride
Chloride
Sphaerium simile - 96 hr
ASTM E729
Lampsilis siliquoidea 96 hr
ASTM 2455-1 )h
Hyalella azteca - 96 hr
ASTM E729
Musculium transversum - 96 hr
ASTM l!7:w
Megalonaias nervosa - 96 hr
ASTM 2455-i)fi
Test Species and Duration
Sulfate
Ligumia recta - 96 hr
ASTM 2455-06
Megalonaias nervosa - 96 hr
ASTM 2455-06
7
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Table 3. Chemical properties of de-chlorinated Lake Michigan (city of Traverse
City) water.
Level of Detection
2/12/2010 Result
2/16/2010 Result
Parameter
(mg/L)
(mg/L)
(mg/L)
Total Aluminum
0.05
ND
0.05
Dissolved Aluminum
0.05
ND
ND
Total Antimony
0.002
ND
ND
Total Arsenic
0.002
0.003
ND
Total Barium
0.05
ND
ND
Total Beryllium
0.001
ND
ND
Total Cadmium
0.001
ND
ND
Total Calcium
0.1
42.6
32.8
Total Chromium
0.005
ND
ND
Total Cobalt
0.02
ND
ND
Total Copper
0.004
ND
ND
Total Iron
0.05
ND
ND
Total Lead
0.002
ND
ND
Total Magnesium
5.0
13.7
12.3
Total Manganese
0.05
ND
ND
Total Mercury
0.0005
ND
ND
Total Nickel
0.05
ND
ND
Total Potassium
0.1
1.42
1.33
Total Selenium
0.005
0.006
ND
Total Silver
0.001
ND
ND
Total Sodium
0.5
8.83
7.75
Total Thallium
0.002
ND
ND
Total Vanadium
0.004
ND
ND
Total Zinc
0.05
ND
ND
ND = Below level of detection.
8
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Table 4. Analytical chemistry methods for each toxicity test completed by GLEC
and INHS.
Test Species and Duration
Nitrate
Nitrite
Ceriodaphnia dubia - 48 hr
SM 4500 N03 and SM 4500 NOz
Lampsilis siliquoidea 96 hr
EPA 353.1 and EPA 354.1
EPA 353.1 and EPA 354.1
Chironomus dilutus - 48 hr
SM 4500 N03 and SM 4500 N02
SM 4500 N03 and SM 4500 NOz
Amphinemura delosa - 96 hr
EPA 353.1 and EPA 354.1
EPA 353.1 and EPA 354.1
Sphaerium simile - 96 hr
EPA 353.1 and EPA 354.1
EPA 353.1 and EPA 354.1
Hyalella azteca - 96 hr
SM 4500 N03 and SM 4500 N02
SM 4500 N03 and SM 4500 NOz
Pimephales promelas - 96 hr
SM 4500 N03 and SM 4500 N02
SM 4500 N03 and SM 4500 NOz
Pimephalespromelas -32 day
SM 4500 N03 and SM 4500 NOz
SM 4500 N03 and SM 4500 NOz
Ceriodaphnia dubia - 7 day
SM 4500 N03 and SM 4500 NOz
Megalonaias nervosa - 96 hr
EPA 353.1 and EPA 354.1
Test Species and Duration
Boron
Manganese
Ceriodaphnia dubia - 48 hr
Lampsilis siliquoidea 96 hr
ICPMS 200.8
ICPMS 200.7
Pimephales promelas - 96 hr
EPA 200.8
Pimephales promelas -32 day
EPA 200.8
Ceriodaphnia dubia - 48 hr (pH 7.75)
EPA 200.8
Pimephales promelas - 96 hr (pH 6.75)
EPA 200.8
Pimephales promelas - 96 hr (pH 7.75)
EPA 200.8
Pimephales promelas - 96 hr (pH 8.75)
EPA 200.8
Ligumia recta - 96 hr
ICPMS 200.8
Megalonaias nervosa - 96 hr
ICPMS 200.8
ICPMS 200.7
Test Species and Duration
Fluoride
Chloride
Sphaerium simile - 96 hr
EPA 300.0
Hyalella azteca - 96 hr
EPA 300.0
Musculium transversum - 96 hr
EPA 300.0
Test Species and Duration
Sulfate
Ligumia recta - 96 hr
EPA 300.0
Megalonaias nervosa - 96 hr
EPA 300.0
9
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CHEMICALS TESTED AND TOXICITY TESTS PERFORMED
Nitrate
Table 5 provides a summary of estimated LC50 values for the eight toxicity tests
performed using nitrate. LC50 values ranged between 16.4 and 937 mg NO3-N/L.
Table 5. LC50 estimates for toxicity tests performed using nitrate.
Test Species and Duration
LC50 (mg NO3-N/L)
Lampsilis siliquoidea 96 hr
357
Chironomus dilutus - 48 hr
278
Amphinemura delosa - 96 hr
456
Sphaerium simile - 96 hr
371
Hyalella azteca - 96 hr
16.4
Pimephalespromelas - 96 hr
415
Pimephalespromelas - 32 day
76.8
Megalonaias nervosa - 96 hr
937
For each of the acute toxicity tests completed using nitrate, two tables were generated:
the first summarizes the test results for each toxicity test, including nominal and
analytical test concentration and LC50 estimates with confidence intervals; the second
table summarizes analytical chemistry data collected throughout the toxicity tests. The
results of chronic tests performed with nitrate were summarized in three tables: the first
summarizes nominal and analytical test concentrations, LC50 estimates with confidence
intervals, NOEC and LOEC estimates, mean survival and mean biomass; the second table
summarizes replicate-specific survival and growth data and the third table summarizes
analytical chemistry data collected throughout the toxicity tests. Also discussed, if
applicable, are deviations from the guidance provided in the ASTM method used to
complete the toxicity testing.
Nitrate and nitrite concentration data generated by the GLEC analytical chemistry
laboratory were reported as nitrate (mg NO3/L) and nitrite (mg NO2/L). For consistency
(i.e. so that the data correspond to the nitrate and nitrogen data generated by the
analytical chemistry laboratory used by the INHS), the GLEC data were converted to
nitrate-nitrogen (N-NO3/L) and nitrite-nitrogen (N-N02/L) for LC50 determinations and
reporting purposes. The data as reported by the GLEC analytical laboratory are available
in the appendices.
10
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96-hr Toxicity of Nitrate on Lamysilis siliguoidea
The 96-hr test to determine the toxicity of nitrate on L. siliquoidea was completed by
INHS. Test organisms, < 5-day old juveniles collected from the Missouri State University
laboratory culture, were acclimated to the dilution water (MHRW), test temperature and
other test conditions prior to test initiation. Once acclimated, test organisms were
examined for any disease, stress, parasites, etc. If free from ailments, test organisms were
randomly assigned to the test chambers (which were randomly assigned to testing
locations); four replicates were used per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 1,129, 564, 282, 141, and 70
mg N-NO3/L.
Testing was conducted at 20 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 6; test results are provided in Table 7. Analytical chemistry data are provided in
Table 8. Accompanying information, including raw laboratory data, analytical chemistry
data and statistical analyses, is provided in Appendix 1.
11
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Table 6. Test conditions for 96-hour toxicity test on Lampsilis siliquoidea with
nitrate.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Lampsilis siliquoidea, juveniles <5 days old, Missouri State
University
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
June 03 - 07,2009
4. Test Temperature (°C):
20+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
50 mL beaker
9. Volume of Test Solutions:
40 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg N-NO3/L):
1,129, 564,282,141, and 70
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination- mg
N-NO3/L):
1,144, 580,291,143, and 74
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Sodium Nitrate: Sigma Aldrich, > 99.0%, ACS Reagent,
Cas. No. 7631-99-4, Lot # B0110978
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
12
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Table 7. Test results for 96-hour toxicity test on Lampsilis siliquoidea with nitrate.
Results of a Lampsilis siliauoidea 96-Hour Static Acute Toxicity Test
Conducted 06/03/09 - 06/07/09 Using: Sodium Nitrate: Sisma Aldrich Cas. No. 7631-99-4
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
5
10
10
10
815 598 491 357
96-Hour LCsn* = 357 mg/L
70 (74) mg/L
0
0
15
25
141 (143) mg/L
0
5
5
25
LC50 9 5% Confidence Limits
282 (291) mg/L
5
10
20
35
24-Hr
48-Hr
72-Hr
96-Hr
564 (580) mg/L
15
30
55
75
LL 730 498 383 250
UL 909 717 631 509
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
1129(1144) mg/L
85
100
100
100
Method(s) Used to Determine LC50 Confidence
Limit Values: Spearman-Karber
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
13
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Table 8. Analytical chemistry data for 96-hour toxicity test on Lampsilis siliquoidea
with nitrate.
Nominal (Measured) Test
Concentration N-N02a N-N03a'b Temperature pH D.O. Conductivity Alkalinity Hardness
(mg/L) (mg/L) (°C) (s.u.) (mg/L) (jimhos) (mg/L) (mg/L)
Dilution water/Control
Day 0
<0.01
<0.01
20.1
8.0
7.81
305
60
90
Day 1
19.9
Day 2
20.0
Day 3
20.1
Day 4
<0.01
<0.01
19.9
8.0
7.77
310
62
92
na
70 (74) mg/L
Day 0
<0.01
73
20.1
7.9
8.01
912
60
90
Day 1
19.9
Day 2
20.0
Day 3
20.0
Day 4
<0.01
75
19.9
8.0
7.82
920
62
92
74
141 (143) mg/L
Day 0
<0.01
144
20.1
7.9
8.00
1484
60
90
Day 1
19.8
Day 2
20.1
Day 3
20.1
Day 4
<0.01
142
19.9
8.0
7.85
1500
62
92
148
143
282 (291) mg/L
Day 0
<0.01
283
20.0
7.9
8.05
2610
60
90
Day 1
19.8
Day 2
20.0
Day 3
20.1
Day 4
<0.01
299
19.9
8.0
7.72
2620
62
92
291
564 (580) mg/L
Day 0
<0.01
582
20.0
7.9
8.10
4760
60
90
Day 1
19.8
Day 2
20.0
Day 3
20.0
Day 4
<0.01
579
20.0
7.9
7.86
4770
62
92
580
1129 (1144) mg/L
Day 0
<0.01
1158
20.0
7.9
8.12
8720
60
90
Day 1
19.9
Day 2
20.0
Day 3
20.1
Day 4
<0.01
1131
19.9
7.9
7.81
8720
62
92
1144
aNitrite Analysis Method 354.1
bNitrite + Nitrate Analysis Method 353.1
na = not applicable
14
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96-hr Toxicity of Nitrate on Meealonaias nervosa
The 96-hr test to determine the toxicity of nitrate onM nervosa was completed by INHS.
Test organisms, < 5-day old juveniles collected from the Genoa National Fish Hatchery
culture, were acclimated to the dilution water (MHRW), test temperature and other test
conditions prior to test initiation. Once acclimated, test organisms were examined for any
disease, stress, parasites, etc. If free from ailments, test organisms were randomly
assigned to the test chambers (which were randomly assigned to testing locations); four
replicates were used per treatment with five organisms per replicate. In one replicate of
the 282 mg/L treatment, a test organism was inadvertently crushed, but this was
accounted for in the LC50 calculation.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 2,258, 1,129, 564, 282, and
141 mg N-NO3/L.
Testing was conducted at 20 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 9; test results are provided in Table 10. Analytical chemistry data are provided in
Table 11. Accompanying information, including raw laboratory data, analytical chemistry
data and statistical analyses, is provided in Appendix 2.
15
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Table 9. Test conditions for 96-hour toxicity test on Megalonaias nervosa with
nitrate.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Megalonaias nervosa, juveniles <5 days old (Genoa
National Fish Hatchery)
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
October 23 - 27,2009
4. Test Temperature (°C):
20+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
50 mL beaker
9. Volume of Test Solutions:
40 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg N-N03/L):
2,258,1,129, 564, 282, and 141
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination- N-
N03/L):
2,002,1,108, 533, 279, and 138
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Sodium Nitrate: Sigma Aldrich, > 99.0%, ACS Reagent,
Cas. No. 7631-99-4, Lot # B0110978
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
16
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Table 10. Test results for 96-hour toxicity test on Megalonaias nervosa with nitrate.
Results of a Mesalonaias nervosa 96-Hour Static Acute Toxicity Test
Conducted 06/03/09 - 06/07/09 Using: Sodium Nitrate: Sisma Aldrich Cas. No. 7631-99-4
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
5
5
5
5
1,203 1,035 969 937
96-Hour LC50* = 937 mg/L
141 (138) mg/L
0
0
0
0
282 (279) mg/L
0
0
0
0
LC50 9 5% Confidence Limits
564 (533) mg/L
0
0
0
0
24-Hr
48-Hr
72-Hr
96-Hr
1129(1108) mg/L
40
55
65
70
LL 991 893 841 818
UL 1,460 1,199 1,116 1,073
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
2258 (2002) mg/L
90
100
100
100
Method(s) Used to Determine LC50 Confidence
Limit Values: Spearman-Karber
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
17
-------
Table 11. Analytical chemistry data for 96-hour toxicity test on Megalonaias nervosa
with nitrate.
Nominal (Measured) Test
Concentration
N-N02"
N-N03a'b
Temp.
PH
D.O.
Cond.
Alkalinity
Hardness
Ammonia
(mg/L)
(mg/L)
(°C)
(S.U.)
(mg/L)
(mmos)
(mg/L)
(mg/L)
(mg/L)
Day 0
<0.02
<0.07
20.9
8.0
8.34
303
60
90
<0.05
Day 1
20.9
8.0
8.13
304
Day 2
20.8
8.1
8.26
306
Day 3
20.9
8.0
8.36
305
Day 4
<0.02
<0.07
20.8
8.0
8.08
315
60
92
<0.05
Dilution water/Control
na
Day 0
<0.02
132.6
20.8
8.2
8.34
1454
60
90
<0.05
Day 1
20.9
8.1
8.06
1421
Day 2
20.8
8.1
8.34
1383
Day 3
20.9
7.8
8.34
1370
Day 4
<0.02
143.4
20.9
8.0
7.92
1361
60
90
<0.05
141 (138) mg/L
138
Day 0
<0.02
275.6
20.8
8.2
8.35
2550
60
90
<0.05
Day 1
20.9
8.1
8.15
2520
Day 2
20.8
8.1
8.25
2470
Day 3
20.9
7.9
8.34
2480
Day 4
<0.02
283.4
20.9
8.0
7.95
2480
62
90
<0.05
282 (279) mg/L
279
Day 0
<0.02
543.3
20.9
8.2
8.35
4680
60
90
<0.05
Day 1
20.9
8.1
8.08
4610
Day 2
20.8
8.1
8.16
4560
Day 3
20.9
7.9
8.32
4560
Day 4
<0.02
523.7
20.9
8.0
7.92
4610
62
92
<0.05
564 (533) mg/L
533
Day 0
<0.02
1189.4
20.9
8.1
8.39
8710
60
90
<0.05
Day 1
20.9
8.1
8.15
8650
Day 2
20.8
8.0
8.24
8530
Day 3
20.9
8.0
8.30
8440
Day 4
<0.02
1032.8
20.9
8.0
7.96
8540
62
92
<0.05
112 (1108) mg/L
1108
Day 0
<0.02
2011.8
20.9
8.1
8.43
16240
60
90
<0.05
Day 1
20.9
8.1
8.16
16000
Day 2
20.8
8.0
8.16
15690
Day 3
20.9
8.0
8.25
15810
Day 4
<0.02
1992.2
20.9
8.0
7.90
15910
64
92
<0.05
2258 (2002) mg/L
2002
aNitrite Analysis Method 354.1
bNitrite + Nitrate Analysis Method 353.1
na = not applicable
temp. = temperature; Cond. = conductivity
18
-------
48-hr Toxicity of Nitrate on Chironomus dilutus
The 48-hr test to determine the toxicity of nitrate on C. dilutus was completed by GLEC.
Test organisms, 10-days old collected from the GLEC laboratory culture, were
acclimated to the dilution water (MHRW), test temperature and other test conditions prior
to test initiation. Once acclimated, test organisms were examined for any disease, stress,
parasites, etc. If free from ailments, test organisms were randomly assigned to the test
chambers (which were randomly assigned to testing locations); four replicates were used
per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 88, 146, 244, 406, and 678
mg N-NO3/L.
Testing was conducted at 22 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Probit method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 12; test results are provided in Table 13. Analytical chemistry data are provided
in Table 14. Accompanying information, including raw laboratory data, analytical
chemistry data, reference toxicant data and statistical analyses, is provided in Appendix
3.
19
-------
Table 12. Test conditions for 48-hour toxicity test on Chironomus dilutus with
nitrate.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Chironomus dilutus, 10 days old, GLEC culture
2. Test Type and Duration:
Static, 48 hours
3. Test Dates:
July 23-July 25,2009
4. Test Temperature (°C):
22+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
150 mL beaker
9. Volume of Test Solutions:
100 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Target or Nominal Test Concentrations (mg N-
N03/L):
678, 406, 244, 146, and 88
14. Analytical Test Concentrations (average of samples
collected at test initiation and termination- mg N-
N03/L):
621,372,255, 166, and 91
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Sodium Nitrate: Sigma Aldrich, > 99.0%, ACS Reagent
Cas. No. 7631-99-4, Batch # 66696LJ
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
20
-------
Table 13. Test results for 48-hour toxicity test on Chironomus dilutus with nitrate.
Results of a Chironomus dilutus (teutons) 48-Hour Static Acute Toxicity Test
Conducted 07/23/09 - 07/25/09 Using: Nitrate (Sodium Nitrate: Sisma Aldrich Cas. No. 7631-99-4)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50* Values (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
>621 278 NA NA
48-Hour LCS0* = 278 mg/L
88 (91) mg/L
0
(0)
0
(0)
146 (166) mg/L
0
(0)
15
(15)
LC50* 95% Confidence Limits
244 (255) mg/L
15
(15)
45
(45)
24-Hr
48-Hr
72-Hr
96-Hr
407 (372) mg/L
20
(20)
65
(65)
LL NA 239 NA NA
UL NA 325 NA NA
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
678 (621) mg/L
25
(25)
100
(100)
Method(s) Used to Determine LC50 Confidence
Limit Values: Probit
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on the measured concentrations.
21
-------
Table 14. Analytical chemistry data for 48-hour toxicity test on Chironomus dilutus
with nitrate.
Nominal (Measured) Test
Concentration
Nitrate"
(mq N03-N/L)
Nitrite3
(mq N02-N/L)
Temperature
(°C)
PH
(s.u.)
D.O.
(mq/L)
Conductivity
(mmhos)
Alkalinity
(mq/L)
Hardness
(mq/L)
Ammonia
(mq/L)
Dilution
water/Control
Day 0
<0.0012
<0.0011
22.3
7.98
7.4
292
60
84
ND
<0.0012
Day 1
21.3
7.94
7.7
Day 2
<0.0012
0.0084
22.4
7.84
7.6
290
90
124
0.0
88 (91) mg/L
Day 0
83.6
<0.0011
22.1
7.99
7.5
854
Day 1
21.3
7.90
7.7
Day 2
98.3
0.0150
22.4
7.87
7.7
860
90.9
146 (166) mg/L
Day 0
187.9
<0.0011
22.1
7.98
7.6
1368
Day 1
21.3
7.90
7.7
Day 2
143.7
0.0072
22.3
7.89
7.5
1304
165.8
244(255) mg/L
Day 0
255.3
0.0016
22.6
7.95
7.6
2030
0.0013
Day 1
21.4
7.86
7.7
Day 2
255.9
0.0110
22.3
7.94
7.5
2000
255.6
407 (372) mg/L
Day 0
325.3
0.0024
22.7
7.96
7.7
2860
Day 1
21.4
7.85
7.8
Day 2
419.0
0.0112
22.3
7.93
7.7
2740
372.1
678 (621) mg/L
Day 0
607.6
0.0025
22.2
7.95
7.9
4900
60
120
ND
Day 1
21.4
7.8
8.0
Day 2
634.7
0.0173
22.3
7.93
7.5
4870
64
136
621.2
a Nitrite Analysis Method SM 4500 N02
b Nitrate Analysis Method SM 4500 N03
ND Not Detect; below detection limit
22
-------
96-hr Toxicity of Nitrate on Amyhinemura delosa
The 96-hr test to determine the toxicity of nitrate on A. delosa was completed by INHS.
Test organisms, field collected nymphs, were acclimated to the dilution water (MHRW),
test temperature and other test conditions prior to test initiation. Once acclimated, test
organisms were examined for any disease, stress, parasites, etc. If free from ailments, test
organisms were randomly assigned to the test chambers (which were randomly assigned
to testing locations); four replicates were used per treatment with four organisms per
replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 2,258, 1,129, 564, 282, and
141 mg N-NO3/L.
Testing was conducted at 12 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 15; test results are provided in Table 16. Analytical chemistry data are provided
in Table 17. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 4.
23
-------
Table 15. Test conditions for 96-hour toxicity test on Amphinemura delosa with
nitrate.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Amphinemura delosa, field-collected nymphs
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
May 14- 18,2009
4. Test Temperature (°C):
12+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
250 mL beaker
9. Volume of Test Solutions:
200 mL
10. No. of Test Organisms per Test Vessel:
4
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
16
13. Test Concentrations (mg N-N03/L):
2,258,1,129, 564, 282, and 141
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination- mg
N-N03/L):
2,286,1,230, 596, 274, and 152
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USPEA MHRW
17. Test Material:
Sodium Nitrate: Sigma Aldrich, > 99.0%, ACS Reagent,
Cas. No. 7631-99-4, Lot # B0110978
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
24
-------
Table 16. Test results for 96-hour toxicity test on Amphinemura delosa with nitrate.
Results of a Amvhinemura delosa 96-Hour Static Acute Toxicity Test
Conducted 05/14/09 - 05/18/09 Using: Nitrate (Sodium Nitrate: Sisma Aldrich Cas. No. 7631-99-4)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
0
0
0
1753 584 499 456
96-Hour LCsn* = 456 mg/L
141 (152) mg/L
12.5
12.5
12.5
12.5
282 (274) mg/L
6.25
31.25
37.5
37.5
LC50 9 5% Confidence Limits
564 (596) mg/L
6.25
37.5
43.75
50
24-Hr
48-Hr
72-Hr
96-Hr
1129(1230) mg/L
25
81.25
87.5
93.75
LL 1394 409 352 325
UL 2204 834 706 642
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
2258 (2286) mg/L
68.75
100
100
100
Method(s) Used to Determine LC50 Confidence
Limit Values: Spearman-Karber
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
25
-------
Table 17. Analytical chemistry data for 96-hour toxicity test on Amphinemura delosa
with nitrate.
Nominal (Measured) Test
Concentration N-N02a N-N03a'b Temperature pH D.O. Conductivity Alkalinity Hardness
(mg/L) (mg/L) (°C) (s.u.) (mg/L) (jimhos) (mg/L) (mg/L)
Dilution water/Control
Day 0
<0.08
<0.08
12.5
7.9
8.80
298
60
90
Day 1
12.3
7.9
297
Day 2
12.4
7.9
297
Day 3
12.5
7.8
300
Day 4
<0.07
<0.07
12.8
7.8
9.85
298
60
88
na
141 (152) mg/L
Day 0
<0.08
161
12.5
8.0
8.80
1440
60
92
Day 1
12.4
7.9
1455
Day 2
12.4
7.9
1458
Day 3
12.5
7.9
1460
Day 4
<0.08
143
12.8
7.9
9.57
1441
60
92
152
282 (274) mg/L
Day 0
<0.08
274
12.5
8.0
9.09
2540
60
92
Day 1
12.3
7.9
2560
Day 2
12.4
7.9
2560
Day 3
12.5
7.9
2560
Day 4
<0.07
274
12.8
7.9
9.92
2540
62
92
274
564 (596) mg/L
Day 0
<0.08
599
12.5
8.0
9.09
4690
60
92
Day 1
11.9
7.9
4690
Day 2
12.4
7.9
4700
Day 3
12.5
7.9
4710
Day 4
<0.07
593
12.8
7.9
9.93
4650
60
92
596
1129 (1230) mg/L
Day 0
<0.08
1260
12.5
8.0
9.11
8720
60
92
Day 1
11.9
7.9
8770
Day 2
12.4
7.9
8780
Day 3
12.5
7.9
8790
Day 4
<0.07
1200
12.8
7.9
9.92
8670
62
92
1230
2258 (2286) mg/L
Day 0
<0.08
2200
12.5
7.9
9.08
16260
60
92
Day 1
12.0
7.9
16270
Day 2
12.4
7.9
16270
Day 3
12.5
7.9
16280
Day 4
<0.07
2375
12.8
7.9
9.97
16230
60
92
2286
aNitrite Analysis Method 354.1
bNitrite + Nitrate Analysis Method 353.1
na = not applicable
26
-------
96-hr Toxicity of Nitrate on Sphaerium simile
The 96-hr test to determine the toxicity of nitrate on S. simile was completed by INHS.
Test organisms, juveniles released from field-collected adults, were acclimated to the
dilution water (MHRW), test temperature and other test conditions prior to test initiation.
Once acclimated, test organisms were examined for any disease, stress, parasites, etc. If
free from ailments, test organisms were randomly assigned to the test chambers (which
were randomly assigned to testing locations); four replicates were used per treatment with
five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 1,129, 564, 282, 141, and 71
mg N-NO3/L.
Testing was conducted at 22 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 18; test results are provided in Table 19. Analytical chemistry data are provided
in Table 20. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 5.
27
-------
Table 18. Test conditions for 96-hour toxicity test on Sphaerium simile with nitrate.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Sphaerium simile, juveniles (released from field-collected
adults)
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
July 21-25, 2009
4. Test Temperature (°C):
22 ±1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
150 mL beaker
9. Volume of Test Solutions:
120 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg N-N03/L):
1,129, 564,282,141, and 71
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination- mg
N-N03/L):
1,125, 569,272,132, and 65
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USPEA MHRW
17. Test Material:
Sodium Nitrate: Sigma Aldrich, > 99.0%, ACS Reagent,
Cas. No. 7631-99-4, Lot # B0110978
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
28
-------
Table 19. Test results for 96-hour toxicity test on Sphaerium simile with nitrate.
Results of a Svhaerium simile 96-Hour Static Acute Toxicity Test
Conducted 07/21/09 - 07/25/09 Using: Sodium Nitrate: Sisma Aldrich Cas. No. 7631-99-4
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
0
0
0
>1125 652 386 371
96-Hour LCsn* = 371 mg/L
71 (65) mg/L
0
0
5
5
141 (132) mg/L
0
0
5
10
LC50 9 5% Confidence Limits
282 (272) mg/L
0
0
5
5
24-Hr
48-Hr
72-Hr
96-Hr
564 (569) mg/L
0
40
100
100
LLNR 508 NR 323
ULNR 838 NR 426
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
1129 (1125) mg/L
0
85
100
100
Method(s) Used to Determine LC50 Confidence
Limit Values: Spearman-Karber
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
29
-------
Table 20. Analytical chemistry data for 96-hour toxicity test on Sphaerium simile
with nitrate.
Nominal (Measured) Test
Concentration N-N02a N-N03a'b Temperature pH D.O. Conductivity Alkalinity Hardness
(mg/L) (mg/L) (°C) (s.u.) (mg/L) (jimhos) (mg/L) (mg/L)
Dilution water/Control
Day 0
-------
96-hr Toxicity of Nitrate on Hyalella azteca
The 96-hr test to determine the toxicity of nitrate on H. azteca was completed by GLEC.
H. azteca were collected from GLEC's laboratory culture. These organisms are
maintained in 10 gallon glass aquaria; plastic artificial turf and screen mesh serve as a
substrate for the culture. The tanks are filled with de-chlorinated Lake Michigan water
(City of Traverse City, Michigan water passed through an activated carbon filter).
Cultures are fed 50 mL of 4 g/L Tetrafin slurry daily. When visible algae are not
observed within the glass aquaria, algae {Selenastrum sp.) are used as a supplement to the
Tetrafin slurry. Additionally, on occasion, dried Aspen (Populus sp.) leaves are prepared
as a food supplement. The culture is maintained in a 16-hour light: 8-hour dark
photoperiod at a temperature between 23 and 26 °C.
Test organisms were acclimated to the dilution water (MHRW), test temperature and
other test conditions prior to test initiation. Once acclimated, test organisms were
examined for any disease, stress, parasites, etc. If free from ailments, test organisms were
randomly assigned to the test chambers (which were randomly assigned to testing
locations); four replicates were used per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 1.8, 2.9, 4.9, 8.1, 13.6, and
22.6 mg N-NO3/L.
Testing was conducted at 22 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 was with the Probit
(48 and 72-hour) and Trimmed Spearman-Karber (96-hour) methods.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 21; test results are provided in Table 22. Analytical chemistry data are provided
in Table 23. Accompanying information, including raw laboratory data, analytical
chemistry data, reference toxicant data and statistical analyses, is provided in Appendix
6.
31
-------
Table 21. Test conditions for 96-hour toxicity test on Hyalella azteca with nitrate.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Hyalella azteca, 10 days old, GLEC culture
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
August 6-August 10, 2009
4. Test Temperature (°C):
22+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
150 mL beaker
9. Volume of Test Solutions:
100 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Target or Nominal Test Concentrations (mg N-
N03/L):
22.6, 13.6, 8.1, 4.9,2.9, and 1.8
14. Analytical Test Concentrations (average of samples
collected at test initiation and termination- mg N-
N03/L):
25.8, 15.2, 9.1, 5.7, 3.2, and 2.1
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USPEA MHRW
17. Test Material:
Sodium Nitrate: Sigma Aldrich, > 99.0%, ACS Reagent
Cas. No. 7631-99-4, Batch # 66696LJ
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
32
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Table 22. Test results for 96-hour toxicity test on Hyalella azteca with nitrate.
Results of a Hvalella arleca 96-Hour Static Acute Toxicity Test
Conducted 08/06/09 - 08/10/09 Using: Nitrate (Sodium Nitrate: Sisma Aldrich Cas. No. 7631-99-4)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 *Values (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
0
(0)
0
(0)
>25.8 28 23 16.4
96-Hour LCS0* = 16.4 mg/L
1.8 (2.1) mg/L
0
(0)
10
(10)
10
(10)
10
(10)
2.9 (3.2) mg/L
0
(0)
0
(0)
5
(5)
10
(10)
LC50 95% Confidence Limits
4.9 (5.7) mg/L
0
(5)
0
(0)
0
(0)
15
(15)
24-Hr
48-Hr
72-Hr
96-Hr
8.1 (9.1) mg/L
0
(10)
15
(15)
15
(15)
20
(20)
LL NA 20.7 17.3 13.3
ULNA 56.2 38.9 20.0
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
13.6 (15.2) mg/L
10
(15)
10
(10)
20
(20)
25
(25)
22.6 (25.8) mg/L
30
(60)
50
(75)
65
(65)
90
(90)
Method(s) Used to Determine LC50 Confidence
Limit Values: Trimmed Spearman-Karber
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
33
-------
Table 23. Analytical chemistry data for 96-hour toxicity test on Hyalella azteca with
nitrate.
Nominal (and M
Test Concent
easured)
rations
Nitrate"
(mg N03-N/L)
Nitrite3
(mg N02-N/L)
Temperature
(°C)
PH
(S.U.)
D.O.
(mg/L)
Conductivity
(mmhos)
Alkalinity
(mg/L)
Hardness
(mg/L)
Ammonia
(mg/L)
Dilution
water/Control
Day 0
<0.0011
<0.0011
22.0
8.00
8.4
300
60
80
ND
Day 1
22.0
7.98
8.5
Day 2
22.7
8.26
8.2
Day 3
22.3
8.07
8.2
Day 4
0.0443
0.0201
21.9
7.89
8.0
290
60
80
0.0560
0.0208
0.050
1.8 (2.1) mg/L
Day 0
2.13
<0.0011
22.0
8.06
8.4
352
Day 1
2.16
22.0
7.99
8.6
Day 2
22.7
8.25
8.7
Day 3
22.5
8.02
8.2
Day 4
2.15
0.0062
22.1
7.84
8.0
327
2.1
2.9(3.2) mg/L
Day 0
2.93
<0.0011
22.0
8.08
8.4
362
Day 1
22.1
7.95
8.6
Day 2
22.7
8.10
8.7
Day 3
22.8
8.04
8.3
Day 4
3.40
0.0067
22.2
7.84
8.0
330
3.2
4.9(5.7) mg/L
Day 0
5.68
<0.0036
22.0
8.10
8.4
377
Day 1
22.1
7.97
8.6
Day 2
22.7
8.09
8.8
Day 3
22.9
8.09
8.5
Day 4
5.77
0.0051
22.2
7.80
8.0
356
5.7
8.1 (9.1) mg/L
Day 0
9.76
<0.0011
22.0
8.07
8.4
402
Day 1
22.1
8.00
8.6
Day 2
22.7
8.08
8.9
Day 3
22.9
8.11
8.7
Day 4
8.52
0.0062
22.2
7.80
8.0
390
9.1
13.6(15.2) mg/L
Day 0
15.52
<0.0011
22.0
8.07
8.4
443
Day 1
22.1
8.02
8.5
Day 2
22.7
8.09
9.0
Day 3
22.9
8.07
8.7
Day 4
14.89
0.4104
22.2
7.80
8.0
400
15.2
22.6 (25.8) mg/L
Day 0
27.45
<0.0011
22.0
8.04
8.4
513
60
80
ND
Day 1
22.1
8.01
8.5
Day 2
22.7
8.06
9.0
Day 3
22.9
8.06
8.9
Day 4
24.22
0.0133
22.1
7.84
8.0
500
60
84
25.8
a Nitrite Analysis Method SM 4500 N02
" Nitrate Analysis Method SM 4500 N03
ND Not Detect; below detection limit
34
-------
96-hr Toxicity of Nitrate on Pimeyhales yromelas
The 96-hr test to determine the toxicity of nitrate on P. promelas was completed by
GLEC. Test organisms, collected from the GLEC laboratory culture, were acclimated to
the dilution water (de-chlorinated Lake Michigan water), test temperature and other test
conditions prior to test initiation. Once acclimated, test organisms were examined for any
disease, stress, parasites, etc. If free from ailments, test organisms were randomly
assigned to the test chambers (which were randomly assigned to testing locations); two
replicates were used per treatment with ten organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 87.9, 146, 244, 407 and 678
mg N-NO3/L.
Testing was conducted at 25 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Probit method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 24; test results are provided in Table 25. Analytical chemistry data are provided
in Table 26. Accompanying information, including raw laboratory data, analytical
chemistry data, reference toxicant data and statistical analyses, is provided in Appendix
7.
35
-------
Table 10. Test conditions for 96-hour toxicity test on Pimephales promelas with
nitrate.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Pimephales promelas, (weight 0.11 g and length 16 mm),
GLEC culture
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
August 6-August 10, 2009
4. Test Temperature (°C):
25+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
4000 mL beaker
9. Volume of Test Solutions:
3500 mL
10. No. of Test Organisms per Test Vessel:
10
11. No. of Test Vessels per Treatment:
2
12. Total No. of Test Organisms per Treatment:
20
13. Target or Nominal Test Concentrations (mg N-
N03/L):
678, 407, 244, 146, and 87.9
14. Analytical Test Concentrations (average of samples
collected at test initiation and termination- mg N-
N03/L):
720, 406, 262, 160, and 98
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
De-chlorinated Lake Michigan Water
17. Test Material:
Sodium Nitrate: Sigma Aldrich, > 99.0%, ACS Reagent
Cas. No. 7631-99-4, Batch # 66696LJ
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
36
-------
Table 25. Test results for 96-hour toxicity test on Pimephales promelas with nitrate.
Results of a Pimephales promelas 96-Hour Static Acute Toxicity Test
Conducted 08/06/09 - 08/10/09 Using: Nitrate (Sodium Nitrate: Sisma Aldrich Cas. No. 7631-99-4)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 * Values (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
0
(0)
0
(0)
>720 580 536 415
96-Hour LCS0* = 415 mg/L
88 (98) mg/L
0
(0)
0
(0)
0
(0)
0
(0)
146 (160) mg/L
0
(0)
0
(0)
0
(0)
5
(5)
LC50* 95% Confidence Limits
244 (262) mg/L
0
(0)
20
(20)
20
(20)
25
(25)
24-Hr
48-Hr
72-Hr
96-Hr
407 (406) mg/L
0
(0)
20
(20)
25
(25)
50
(50)
LL NA 465 437 343
ULNA 841 730 526
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
678 (720) mg/L
35
(35)
65
(65)
70
(70)
80
(80)
Method(s) Used to Determine LC50 Confidence
Limit Values: Probit
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
37
-------
Table 26. Analytical chemistry data for 96-hour toxicity test on Pimephales promelas
with nitrate.
Nominal (and M
Test Concen
easured)
ration
Nitrate"
(mg N03-N/L)
Nitrite3
(mg N02-N/L)
Temperature
(°C)
PH
(s.u.)
D
(m
O.
g/L)
Conductivity
(mmhos)
Alkalinity
(mg/L)
Hardness
(mg/L)
Ammonia
(mg/L)
Dilution
water/Control
Day 0
0.2528
<0.0011
25
8.04
8.4
8.4
310
96
136
ND
Day 1
24.6
7.71
5.9
6.0
Day 2
24.3
7.81
6.6
7.4
Day 3
24.4
8.28
6.7
6.9
Day 4
0.2460
0.0028
24.4
8.00
7.0
7.0
302
100
140
0.2345
24.4
8.04
0.2444
88 (98) mg/L
Day 0
97.5830
<0.0011
25.0
8.10
8.4
8.4
1030
Day 1
24.6
7.68
5.9
5.7
Day 2
24.2
7.77
7.7
6.3
Day 3
24.3
8.11
6.7
6.9
Day 4
97.5830
0.0113
24.4
8.12
6.8
7.0
979
97.5830
24.3
8.14
146(160) mg/L
Day 0
162.1866
<0.0011
25.0
8.09
8.4
8.4
1483
Day 1
24.6
7.68
5.8
5.8
Day 2
24.2
7.75
7.9
6.6
Day 3
24.4
8.11
6.9
6.8
Day 4
156.3135
0.0218
24.3
8.10
6.6
6.7
1429
160.6054
0.0205
24.4
8.14
159.7018
244 (262) mg/L
Day 0
266.7721
0.0026
25.0
8.07
8.4
8.4
2241
Day 1
24.6
7.68
5.8
5.7
Day 2
24.3
7.65
5.9
5.4
Day 3
24.4
8.10
6.5
6.4
Day 4
256.3813
0.0883
24.4
8.05
6.7
6.9
2170
261.5767
24.4
8.03
407 (406) mg/L
Day 0
410.2101
0.0026
25.0
8.07
8.4
8.4
3361
Dav 1
24.6
7.57
5.9
5.8
Day 2
24.4
7.47
6.7
6.0
Dav 3
24.3
8.00
6.2
6.0
Day 4
402.5299
0.1096
24.4
7.94
6.4
6.6
3250
406.3700
24.4
8.02
678 (720) mg/L
Day 0
754.0095
<0.0011
25.0
8.05
8.4
8.4
5550
100
136
ND
Day 1
<0.0011
24.6
7.47
5.9
5.6
Day 2
24.4
7.42
5.9
5.9
Dav 3
24.3
7.88
6.6
6.7
Day 4
685.5658
0.1428
24.4
8.03
6.5
6.6
5240
104
140
719.6747
24.4
7.94
" Nitrite Analysis Method SM 4500 N02
b Nitrate Analysis Method SM 4500 N03
ND Not Detect; below detection limit
38
-------
32-day Toxicity of Nitrate on Pimeyhales yromelas
The 32-day test to determine the toxicity of nitrate on P. promelas was completed by
GLEC. The fish were continuously exposed for 32 days to five concentrations of nitrate
(nominal concentrations of 28.2, 56.5, 113, 226 and 452 mg N-NO3/L) and to a dilution
water control using a continuous flow-through system (Benoit et al. 1982). The
temperature-controlled test concentration solutions were supplied to each test chamber
via the continuous flow-through system at a rate of approximately four turnovers a day.
There were four replicate test chambers for each treatment. The flow through test was
conducted at 25 ± 1 °C with a photoperiod of 16 hr light and 8 hr darkness (ambient
laboratory light).
After test concentrations had achieved steady state in the flow through system, the test
was initiated with < 24 hour old fertilized embryos. The embryos were randomly
assigned to incubation cups until each incubation cup contained 30 embryos. The
incubation cups were randomly assigned to the 2.5 L glass test chambers (1 cup per
chamber) and suspended in the test solutions from a rocker arm assembly. The rocker
arm assembly moves the incubation cups in a reciprocal motion within each test chamber.
Embryos were inspected on a daily basis and the number of live, hatched and dead
embryos was recorded. On Day 5 of the test (three days after first hatch), the surviving
fish were randomly thinned to achieve 20 fish in each test chamber. The remainder of the
surviving fish was discarded. The number of surviving fish was recorded at test
termination (32 days). In addition, the wet weights were recorded for each fish at test
termination. Because of the size range of fish in each test chamber, all of the fish from
each test chamber were weighed together to determine average dry weight.
There were several instances throughout the test when instantaneous water temperature
measurements made within individual replicates exceeded those allowed in the toxicity
testing method. However, incidences of temperature exceedance only occurred
intermittingly and the average water temperatures (across the duration of the test) in each
replicate were always ± 0.4 °C of the target test temperature (25 °C) in all treatments. In
addition, the water temperature as recorded by the continuous temperature logger was
never outside the range outlined in the method. Therefore, these water temperature
exceedances likely had no effect on the results of this toxicity test.
In addition, there was an instance of low DO (4.3 mg/L) in one of the replicates at test
termination. Because this incident occurred on the last day of the test and similarly low
DO concentrations were never observed on any other day of the test, this low DO was an
anomaly and likely had no effect on the results of this toxicity test.
Once the test was complete, the LC50, NOEC, and LOEC values were determined using
the average measured concentrations with the Spearman Karber and ANOVA methods.
LC25, LC20, LC10, EC50, EC25, EC20 and EC 10 values were estimated using EPA's TRAP.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 27; test results are provided in Table 28. Survival and growth data are provided
39
-------
in Table 29 and analytical chemistry data are provided in Table 30. Accompanying
information, including raw laboratory data, analytical chemistry data and statistical
analyses, is provided in Appendix 8.
Table 27. Test conditions for 32-day toxicity test on Pimephales promelas with
nitrate.
Summary of Toxicity Test Conditions
l.
Test Species and Age:
Pimephales promelas, (<24 hour fertilized embryos ),
GLEC culture
2.
Test Type and Duration:
Continuous flow-through; 32 days
3.
Test Dates:
July 24-August 25, 2009
4.
Test Temperature (°C):
25+1
5.
Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6.
Photoperiod:
16 h light, 8 h darkness
7.
Feeding Regime:
Live Brine Shrimp (Artemia nauplii) Twice daily
8.
Size of Test Vessel:
2.5 Liter glass Tank
9.
Volume of Test Solutions:
2,000 mL
10.
No. of Test Organisms per Test Vessel:
30 eggs, thinned to 20 larvae after hatch
11.
No. of Test Vessels per Treatment:
4
12.
Total No. of Test Organisms per Treatment:
120 eggs, thinned to 80 larvae after hatch
13.
Target or Nominal Test Concentrations (mg N-
N03/L):
452, 226, 113, 56.5, and 28.2
14. Analytical Test Concentrations (average of samples
441,226, 109, 48.6, and 21.7
collected at test initiation and termination- mg N-
NO3/L):
15.
Renewal of Test Solutions:
Continuous flow through, 4 turnovers per day
16.
Dilution and Primary Control Water:
De-chlorinated Lake Michigan Water
17. Test Material:
Sodium Nitrate: Sigma Aldrich, > 99.0%, ACS Reagent
Cas. No. 7631-99-4, Batch # 66696LJ
18.
Secondary Control Water:
None
19.
Aeration:
None
20.
Endpoints Measured:
Survival (LC50, LC25 LC20 andLC10,NOEC andLOEC) and
Growth (EC50)EC25)EC20 andEC10,NOEC and LOEC)
40
-------
Table 28. Test results for 32-day toxicity test on Pimephales promelas with nitrate.
Results of a Pimephales promelas 32-Dav Continuous Flow Chronic Toxicity Test
Conducted 07/24/09 - 08/25/09 Usine: Nitrate (Sodium Nitrate: Sisma Aldrich Cas. No. 7631-99-4)
Test Solution Concentrations
Measured
Primary Control/
Dilution Water
22 mg/L
49 mg/L
109 mg/L
226 mg/L
441 mg/L
Embryo Percent Hatch (%)
100
100
100
99
98
99
32-Day Mean Survival (%)
88.8
86.3
92.5
16.3a
10.0a
0a
32-Day Average Biomass1 (mg)
5.44
5.72
5.54
1.91a
0.84a
0a
Embryo Percent Hatch NOEC:
441 mg/L
32-Day LC20
64.6 mg/L (44.7 mg/L - 93.5 mg/L)
32-Day LC50*:
76.8 mg/L (70 mg/L-84 mg/L)
32-Day LC10
55.5 mg/L (34.7 mg/L - 88.7 mg/L)
32-Day Survival NOEC:
49 mg/L
32-Day EC50
91.3 mg/L (72.9 mg/L - 114.3 mg/L)
32-Day Survival LOEC:
109 mg/L
32-Day EC25
65.3 mg/L (44.4 mg/L - 96.1 mg/L)
32-Day Growth NOEC:
49 mg/L
32-Day EC20
59.8 mg/L (38.7 mg/L - 92.4 mg/L)
32-Day Growth LOEC:
109 mg/L
32-Day EC10
46.7 mg/L (26.2 mg/L - 83.4 mg/L)
32-Day LC25:
68.2 mg/L (48.8 mg/L - 95.4 mg/L)
a: Significantly different from dilution water control (p< 0.05)
*: All LC, EC, NOEC and LOEC values are determined based on the average measured nitrate concentration.
NOEC: No-Observed-Effect-Concentration
LOEC: Lowest-Observed-Effect-Concentration
'Biomass: Biomass is the average dry weight of the four replicates calculated by the total dry weight of surviving
organisms divided by the initial number of organisms (20).
41
-------
Table 29. Survival and growth data for 32-day toxicity test on Pimephales promelas
with nitrate.
Nominal (and Measured) Test
Concentration
Number of
Eggs at Test
Initiation
Number of
Hatched
Larvae
Number of
Dead Eggs
Percent
Hatched
Larvae
Number of Larvae
at Test
Termination
Percent Survival at Test
Termination*
Biomass1 (mg)
Dilution
water/Control
Replicate # 1
30
30
0
100.0
18
90.0
5.34
Replicate # 2
30
30
0
100.0
18
90.0
5.94
Replicate # 3
30
30
0
100.0
20
100.0
5.65
Replicate # 4
30
30
0
100.0
15
75.0
4.79
Average 100.0 88.8 5.43
28 (22) mg/L
Replicate # 1
30
30
0
100.0
18
90.0
6.62
Replicate # 2
30
30
0
100.0
15
75.0
5.67
Replicate # 3
30
30
0
100.0
18
90.0
5.34
Replicate # 4
30
30
0
100.0
18
90.0
5.26
Average 100.0 86.3 5.72
56 (49) mg/L
Replicate # 1
30
30
0
100.0
19
95.0
5.45
Replicate # 2
30
30
0
100.0
20
100.0
5.98
Replicate # 3
30
30
0
100.0
20
100.0
5.25
Replicate # 4
30
30
0
100.0
15
75.0
5.46
Average 100.0 92.5 5.54
113(109) mg/L
Replicate # 1
30
30
0
100.0
3
15.0
1.78
Replicate # 2
30
29
1
96.7
4
20.0
1.84
Replicate # 3
30
30
0
100.0
4
20.0
2.79
Replicate # 4
30
30
0
100.0
2
10.0
1.25
Average 99.2 16.3 1.91
226 (226) mg/L
Replicate # 1
30
29
1
96.7
1
5.0
0.20
Replicate # 2
30
30
0
100.0
4
20.0
2.02
Replicate # 3
30
29
1
96.7
2
10.0
1.04
Replicate # 4
30
30
0
100.0
1
5.0
0.10
Average 98.3 10.0 0.84
452 (441) mg/L
Replicate # 1
30
29
1
96.7
0
0.0
0.00
Replicate # 2
30
30
0
100.0
0
0.0
0.00
Replicate # 3
30
30
0
100.0
0
0.0
0.00
Replicate # 4
30
30
0
100.0
0
0.0
0.00
Average 99.2 0.0 0.00
* On Day 5 of the test, (three days after first hatch) the surviving fish were randomly thinned to 20 fish in each test chamber. Percent surval at test
termination is the number of surviving at test termination divided by 20.
Biomass: Biomass is the total dry weight of surviving organisms divided by the initial number of organisms (20)
42
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Table 30. Analytical chemistry data for 32-day toxicity test on Pimephales promelas
with nitrate.
Nominal (and Measured) T est
Conent rations
no3
(mg N03-
N/L)
no2
(mg N02-
N/L)
Temperature
(°C)
pH
(s.U.)
DO
(mg/L)
SC
(mmos)
Hardness
(mg/L)
Alkalinity
(mg/L)
Ammonia
(mg/L)
Dilution
Water/Control
Average
Replicate #
1
0.17
(0.005-0.258)
0.006
25.3
(24.0-26.3)
7.97
7.4
(7.2-7.8)
298
180
93
ND
Replicate #
2
0.26
(0.221-0.307)
0.003
25.0
(24.2-26.0)
8.13
7.6
(7.2-8.2)
296
ND
Replicate #
3
0.26
(0.226-0.289)
0.003
25.0
(24.1-25.6)
8.17
7.7
(7.3-8.2)
295
ND
Replicate #
4
0.24
(0.210-0.271)
0.003
25.0
(24.2-26.1)
8.24
7.7
(7.2-8.1)
296
ND
0.23
28 mg/L
(22 mg/L)
Average
Replicate #
1
20
(12-40)
0.006
24.9
(24.0-25.8)
8.21
7.6
(6.7-8.4)
442
154
104
ND
Replicate #
2
22
(13-41)
0.003
24.9
(24.1-26.0)
8.22
7.7
(7.1-8.2)
483
ND
Replicate #
3
21
(14-41)
0.006
25.0
(24.2-25.7)
8.27
7.8
(7.3-9.2)
426
ND
Replicate #
4
24
(13-41)
0.006
25.1
(24.2-25.7)
8.32
7.9
(7.2-9.2)
418
ND
22
56 mg/L
(49mg/L)
Average
Replicate #
1
48
(38-65)
0.009
25.0
(24.2-26.0)
8.23
7.6
(6.6-9.1)
626
140
107
ND
Replicate #
2
48
(37-67)
0.006
24.9
(24.1-25.8)
8.22
7.8
(7.4-8.1)
689
ND
Replicate #
3
51
(38-69)
0.006
25.1
(24.3-26.1)
8.16
7.6
(7.1-8.0)
654
ND
Replicate #
4
47
(39-70)
0.006
25.1
(24.2-26.0)
8.23
7.8
(7.0-9.0)
667
ND
49
113 mg/L
(109 mg/L)
Average
Replicate #
1
107
(90-146)
0.009
24.8
(24.0-26.0)
8.27
7.6
(6.8-8.2)
1045
164
101
ND
Replicate #
2
112
(95-141)
0.003
24.6
(23.7-26.0)
8.24
7.7
(7.0-8.3)
1083
ND
Replicate #
3
107
(88-137)
0.003
24.9
(24.2-25.6)
8.26
7.9
(7.4-9.4)
984
ND
Replicate #
4
109
(92-139)
0.006
24.8
(23.7-25.5)
8.26
7.8
(7.1-9.3)
958
ND
109
226 nig/L
(226 mg/L)
Average
Replicate #
1
220
(157-285)
0.006
24.9
(24.2-26.0)
8.24
7.6
(6.9-8.0)
2449
132
104
ND
Replicate #
2
232
(168-300)
0.225
24.7
(23.6-25.9)
8.10
7.2
(4.3-8.0)
2206
ND
Replicate #
3
225
(172-297)
0.006
25.0
(24.2-25.8)
8.25
7.7
(6.6-9.4)
2137
ND
Replicate #
4
227
(158-297)
0.006
25.0
(24.0-25.9)
8.26
7.8
(6.8-9.4)
2240
ND
226
452 mg/L
(441 mg/L)
Average
Replicate #
1
453
(389-485)
0.006
25.1
(24.2-26.0)
8.22
7.5
(6.4-8.1)
3820
146
101
ND
Replicate #
2
421
(341-471)
0.006
24.8
(23.9-26.0)
8.23
7.7
(6.9-8.3)
3763
ND
Replicate #
3
452
(411-493)
0.033
24.8
(24.0-25.7)
8.15
7.5
(6.8-9.4)
4001
ND
Replicate #
4
437
(411-463)
0.009
25.0
(24.1-25.8)
8.19
7.8
(6.2-9.4)
3610
ND
441
DO: Dissolved Oxygen
SC: Specific Conductance
ND: Not detected
43
-------
Nitrite
Table 31 provides a summary of estimated LC50 values for the nine toxicity tests
performed using nitrite. LC50 values ranged between 1.0 and 177 mg NO3-N/L.
Table 31. LC50 estimates for toxicity tests performed using nitrite.
Test Species and Duration
LC50 (mg N02-N/L)
Ceriodaphnia dubia - 48 hr
4.6
Lampsilis siliquoidea 96 hr
111
Chironomus dilutus - 48 hr
15.6
Amphinemura delosa - 96 hr
1.0
Sphaerium simile - 96 hr
55.7
Hyalella azteca - 96 hr
1.5
Pimephalespromelas - 96 hr
27.0
Pimephalespromelas - 32 day
11.3
Ceriodaphnia dubia - 7 day
4.7
For each of the acute toxicity tests completed using nitrite, two tables were generated: the
first summarizes the test results for each toxicity test, including nominal and analytical
test concentration and LC50 estimates with confidence intervals; the second table
summarizes analytical chemistry data collected throughout the toxicity tests. The results
of chronic tests performed with nitrite were summarized in three tables: the first
summarizes nominal and analytical test concentrations, LC50 estimates with confidence
intervals, NOEC and LOEC estimates, mean survival and mean biomass; the second table
summarizes replicate-specific survival and growth data and the third table summarizes
analytical chemistry data collected throughout the toxicity tests. Also discussed, if
applicable, are deviations from the guidance provided in the ASTM method used to
complete the toxicity testing.
Nitrate and nitrite concentration data generated by the GLEC analytical chemistry
laboratory were reported as nitrate (mg NO3/L) and nitrite (mg N02/L). For consistency
(i.e. so that the data correspond to the nitrate and nitrogen data generated by the
analytical chemistry laboratory used by the INHS), the GLEC data were converted to
nitrate-nitrogen (N-NO3/L) and nitrite-nitrogen (N-NO2/L) for LC50 determinations and
reporting purposes. The data as reported by the GLEC analytical laboratory are available
in the appendices.
44
-------
96-hr Toxicity of Nitrite on Lamysilis siliguoidea
The 96-hr test to determine the toxicity of nitrite on L. siliquoidea was completed by
INHS. Test organisms, < 5-day old juveniles collected from the Missouri State University
laboratory culture, were acclimated to the dilution water (MHRW), test temperature and
other test conditions prior to test initiation. Once acclimated, test organisms were
examined for any disease, stress, parasites, etc. If free from ailments, test organisms were
randomly assigned to the test chambers (which were randomly assigned to testing
locations); four replicates were used per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 304, 152, 76, 38, 19, and 9.5
mg N-N02/L.
Testing was conducted at 20 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 32; test results are provided in Table 33. Analytical chemistry data are provided
in Table 34. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 9.
45
-------
Table 32. Test conditions for 96-hour toxicity test on Lampsilis siliquoidea with
nitrite.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Lampsilis siliquoidea, juveniles <5 days old, Missouri State
University
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
September 08 - 12, 2009
4. Test Temperature (°C):
20+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
50 mL beaker
9. Volume and Depth of Test Solutions:
40 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg N-N02/L):
304, 152, 76,38, 19, and 9.5
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination- mg
N-N02/L):
312.8, 158.1,82.0,39.1, 19.6, and 9.9
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Sodium Nitrite: Fisher, certified ACS, assay 99.7%, Lot #
080939
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
46
-------
Table 33. Test results for 96-hour toxicity test on Lampsilis siliquoidea with nitrite.
Results of a Lampsilis siliauoidea 96-Hour Static Acute Toxicity Test
Conducted 09/08/09 - 09/12/09 Using: Sodium Nitrite: Sisma Aldrich Cas. No. 7632-00-0
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
(ECsn*)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
0
0
0
>312.8 >312.8 240.6 177.0
96-Hour LCsn* = 177.0 mg/L
9.5 (9.9) mg/L
0
0
0
10
19.0 (19.6) mg/L
0
0
5
10
LC50 9 5% Confidence Limits
38.0 (39.1) mg/L
0
0
0
0
24-Hr
48-Hr
72-Hr
96-Hr
76.0 (82.0) mg/L
0
0
0
10
LLNR NR 209.9 145.0
ULNR NR 275.7 215.0
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
152.0 (158.1) mg/L
0
0
10
30
304.0 (312.8) mg/L
0
0
75
100
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
47
-------
Table 34. Analytical chemistry data for 96-hour toxicity test on Lanpsilis siliquoidea
with nitrite.
Nominal (Measured) Test
Concentration N-N02a N-N03a'b Temperature pH D.O. Conductivity Alkalinity Hardness
(mg/L) (mg/L) (°C) (s.u.) (mg/L) (mmos) (mg/L) (mg/L)
Dilution water/Control
Day 0
-------
48-hr Toxicity of Nitrite on Chironomus dilutus
The 48-hr test to determine the toxicity of nitrite on C. dilutus was completed by GLEC.
Serial dilutions. Test organisms, 10-days old from the GLEC culture, were acclimated to
the dilution water (MHRW), test temperature and other test conditions prior to test
initiation. Once acclimated, test organisms were examined for any disease, stress,
parasites, etc. If free from ailments, test organisms were randomly assigned to the test
chambers (which were randomly assigned to testing locations); four replicates were used
per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 2.4, 3.9, 6.6, 11,18 and 30
mg N02-N/L.
Testing was conducted at 22 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Probit method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 35; test results are provided in Table 36. Analytical chemistry data are provided
in Table 37. Accompanying information, including raw laboratory data, analytical
chemistry data, reference toxicant data and statistical analyses, is provided in Appendix
10.
49
-------
Table 35. Test conditions for 48-hour toxicity test on Chironomus dilutus with
nitrite.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Chironomus dilutus, 10 days old, GLEC culture
2. Test Type and Duration:
Static, 48 hours
3. Test Dates:
July 23-July 25,2009
4. Test Temperature (°C):
22+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
150 mL beaker
9. Volume of Test Solutions:
100 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Target or Nominal Test Concentrations (mg NO2-
N/L):
30,18, 11, 6.6, 3.9, and 2.4
14. Measured Test Concentrations (average of samples
collected at test initiation and termination- mg NO2-
N/L):
32.4, 19, 11, 6.8, 4.0, and 2.6
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Sodium Nitrite: Sigma Aldrich, 97 +%, ACS Reagent Cas.
No. 7632-00-0, Batch # 08316DJ
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
50
-------
Table 36. Test results for 48-hour toxicity test on Chironomus dilutus with nitrite.
Results of a Chironomus dilutus (teutons) 48-Hour Static Acute Toxicity Test
Conducted 07/23/09 - 07/25/09 Using: Nitrite (Sodium Nitrite: Sisma Aldrich Cas. No. 7632-00-0)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
31.2 15.6 NA NA
48-Hour LCS0* = 15.6 mg/L
2.4 (2.6) mg/L
0
(0)
0
(0)
3.9 (4.0) mg/L
0
(0)
0
(0)
LC50 95% Confidence Limits (mg/L)
6.6 (6.8) mg/L
0
(0)
10
(10)
24-Hr
48-Hr
72-Hr
96-Hr
11 (11) mg/L
0
(0)
20
(20)
LL 25.4 13.2 NA NA
UL 47.5 18.9 NA NA
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
18 (19) mg/L
20
(20)
50
(50)
30 (32) mg/L
50
(50)
100
(100)
Method(s) Used to Determine LC50 Confidence
Limit Values: Probit
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
51
-------
Table 37. Analytical chemistry data for 48-hour toxicity test on Chironomus dilutus
with nitrite.
Nominal (and Measured)
Test Concentrations Nitrite3 Nitrateb Temperature pH D.O. Conductivity Alkalinity Hardness Ammonia
(mg NQ2-N/L) (mg NQ3-N/L) (°C) (s.u.) (mg/L) (mmos) (mg/L) (mg/L) (mg/L)
Dilution
water/Control
Day 0
<0.0011
0.000
22.3
8.04
7.4
292
60
84
ND
0.000
Day 1
21.4
7.78
7.5
Day 2
0.008
<0.0012
22.4
7.80
7.7
290
90
124
ND
0.008
2.4 (2.6) mq/L
Day 0
2.7
0.400
22.7
8.01
7.5
291
2.6
Day 1
21.4
7.80
7.5
Day 2
2.5
0.115
22.4
7.81
7.7
290
2.6
3.9 (4.0) mq/L
Day 0
3.9
0.097
22.4
8.02
7.6
330
Day 1
21.4
7.84
7.5
Day 2
4.1
3.885
22.4
7.85
7.5
334
4.0
6.6 (6.8) mq/L
Day 0
6.7
0.700
22.5
8..05
7.6
381
Day 1
21.4
7.89
7.5
Day 2
6.5
0.339
22.5
7.88
7.5
394
7.1
<0.1129
6.8
11 (11) mq/L
Day 0
10.7
0.971
22.5
8.05
7.6
436
Day 1
21.4
7.89
7.6
Day 2
10.7
0.542
22.3
7.91
7.2
430
10.7
18 (19) mq/L
Day 0
18.3
3.275
22.4
8.05
7.5
478
Day 1
21.4
7.91
7.7
Day 2
18.9
<0.2485
22.3
7.91
7.2
484
18.6
30 (32) mq/L
Day 0
32.9
< 1.13
22.5
8.03
7.7
525
60
80
ND
Day 1
21.4
7.95
7.7
Day 2
32.0
0.949
22.3
7.99
7.3
530
64 116
ND
32.4
I
a Nitrite Analysis Method SM 4500 N02
b Nitrate Analysis Method SM 4500 N03
ND Not Detected; below detection limit
52
-------
96-hr Toxicity of Nitrite on Amyhinemura delosa
The 96-hr test to determine the toxicity of nitrite on A. delosa was completed by INHS.
Test organisms, field-collected nymphs, were acclimated to the dilution water (MHRW),
test temperature and other test conditions prior to test initiation. Once acclimated, test
organisms were examined for any disease, stress, parasites, etc. If free from ailments, test
organisms were randomly assigned to the test chambers (which were randomly assigned
to testing locations); four replicates were used per treatment with five organisms per
replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 12, 6.1, 3.0, 1.5, and 0.8 mg
N-N02/L.
Testing was conducted at 12 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 38; test results are provided in Table 39. Analytical chemistry data are provided
in Table 40. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 11.
53
-------
Table 38. Test conditions for 96-hour toxicity test on Amphinemura delosa with
nitrite.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Amphinemura delosa, nymphs, Field collected
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
May 14- 18,2009
4. Test Temperature (°C):
12+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
250 mL beaker
9. Volume of Test Solutions:
200 mL
10. No. of Test Organisms per Test Vessel:
4
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
16
13. Test Concentrations (mg N-N02/L):
12,6.1,3.0, 1.5, and 0.8
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination- mg
N-N02/L):
13,6.1,3.1, 1.5, and 0.7
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Sodium Nitrite: Fisher, certified ACS, assay 99.7%, Lot #
080939
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
54
-------
Table 39. Test results for 96-hour toxicity test on Amphinemura delosa with nitrite.
Results of aAmvhinemura delosa 96-Hour Static Acute Toxicity Test
Conducted 05/14/09 - 05/18/09 Using: Nitrite (Sodium Nitrite: Sisma Aldrich Cas. No. 7632-00-0)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
(ECsn*)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
6.25
6.25
6.25
3.6 1.7 1.0 1.0
96-Hour LCsn* = 1.0 mg/L
0.8 (0.7) mg/L
12.5
12.5
18.75
25
1.5 (1.5) mg/L
12.5
31.25
81.25
81.25
LC50 9 5% Confidence Limits
3.0 (3.1) mg/L
50
100
100
100
24-Hr
48-Hr
72-Hr
96-Hr
6.1 (6.1) mg/L
68.75
100
100
100
LL 2.2 1.3 0.9 0.8
UL 5.8 2.2 1.2 1.2
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
12.2 (12.7) mg/L
75
100
100
100
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
55
-------
Table 40. Analytical chemistry data for 96-hour toxicity test on Amphinemura delosa
with nitrite.
Nominal (Measured) Test
Concentration N-N02a N-N03a'b Temperature pH D.O. Conductivity Alkalinity Hardness
(mg/L) (mg/L) (°C) (s.u.) (mg/L) (jimhos) (mg/L) (mg/L)
Dilution water/Control
Day 0
<0.08
<0.08
12.5
7.9
9.10
301
60
88
Day 1
12.1
7.9
300
Day 2
12.3
7.9
300
Day 3
12.5
7.9
305
Day 4
<0.07
<0.07
12.8
7.9
9.97
299
60
90
na
0.8(0.7) mg/L
Day 0
0.8
1.0
12.5
7.8
9.17
306
60
90
Day 1
12.1
7.9
303
Day 2
12.3
7.9
302
Day 3
12.5
7.9
303
Day 4
0.7
1.0
12.7
7.9
9.98
307
60
90
0.7
1.5 (1.5) mg/L
Day 0
1.5
2.1
12.5
7.8
9.13
314
60
90
Day 1
12.1
7.9
310
Day 2
12.3
7.9
310
Day 3
12.5
7.9
310
Day 4
1.5
2.0
12.8
7.9
9.93
313
60
90
1.5
3.0 (3.1) mg/L
Day 0
3.1
4.1
12.5
7.8
9.14
327
60
90
Day 1
12.3
7.9
324
Day 2
12.3
7.9
325
Day 3
12.5
7.9
325
Day 4
3.0
3.8
12.7
7.9
9.97
324
60
90
3.1
6.1 (6.1) mg/L
Day 0
6.1
8.9
12.5
7.8
9.06
354
60
90
Day 1
12.3
7.9
351
Day 2
12.3
7.9
355
Day 3
12.5
7.9
350
Day 4
6.1
3.6
12.7
7.9
9.99
351
60
88
6.1
12.2 (12.7) mg/L
Day 0
12.8
18.6
12.5
7.8
9.08
406
60
90
Day 1
12.2
7.9
404
Day 2
12.3
7.9
405
Day 3
12.5
7.9
405
Day 4
12.7
14.5
12.2
7.9
9.99
405
62
90
12.7
aNitrite Analysis Method 354.1
b Nitrite + Nitrate Analysis Method 353.1
na = not applicable
56
-------
96-hr Toxicity of Nitrite on Sphaerium simile
The 96-hr test to determine the toxicity of nitrite on S. simile was completed by INHS.
Test organisms, juveniles released from field-collected adults, were acclimated to the
dilution water (MHRW), test temperature and other test conditions prior to test initiation.
Once acclimated, test organisms were examined for any disease, stress, parasites, etc. If
free from ailments, test organisms were randomly assigned to the test chambers (which
were randomly assigned to testing locations); four replicates were used per treatment with
five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 304, 152, 76, 38, 19, and 9.5
mg N-N02/L.
Testing was conducted at 22 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 41; test results are provided in Table 42. Analytical chemistry data are provided
in Table 42. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 12.
57
-------
Table 41. Test conditions for 96-hour toxicity test on Sphaerium simile with nitrite.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Sphaerium simile, juveniles (released from field-collected
adults)
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
August 17-21,2009
4. Test Temperature (°C):
22+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
150 mL beaker
9. Volume of Test Solutions:
120 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg N-N02/L):
304, 152, 76,38, 19, and 9.5
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination- mg
N-N02/L):
296, 158, 82,39, 19, and 9.4
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Sodium Nitrite: Fisher, certified ACS, assay 99.7%, Lot #
080939
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
58
-------
Table 42. Test results for 96-hour toxicity test on Sphaerium simile with nitrite.
Results of a Svhaerium simile 96-Hour Static Acute Toxicity Test
Conducted 08/17/09 - 08/21/09 Using: Sodium Nitrite: Sisma Aldrich Cas. No. 7632-00-0
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
0
0
0
>296.1 240.0 178.7 55.7
96-Hour LCsn* = 55.7 mg/L
9.5 (9.4) mg/L
0
0
0
0
19.0 (19.2) mg/L
0
0
0
15
LC50 9 5% Confidence Limits
38.0 (38.8) mg/L
0
0
0
40
24-Hr
48-Hr
72-Hr
96-Hr
76.1 (82.2) mg/L
0
0
0
50
LLNR 171.1 65.1 43.0
ULNR 336.7 490.7 72.1
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
152.2 (157.7) mg/L
0
30
50
95
304.4 (296.1) mg/L
0
60
60
100
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
59
-------
Table 43. Analytical chemistry data for 96-hour toxicity test on Sphaerium simile
with nitrite.
Nominal (Measured) Test Concentration N-N02" N-N03"'b Temperature pH D.O. Conductivity Alkalinity Hardness
(mg/L) (mg/L) (°C) (s.u.) (mg/L) (fimhos) (mg/L) (mg/L)
Dilution water/Control
Day 0
<0.02
NM
22.3
8.0
7.74
302
62
88
Day 1
22.9
7.7
7.14
304
Day 2
22.7
7.7
7.17
313
Day 3
22.7
7.7
7.31
316
Day 4
<0.02
NM
22.7
7.7
7.51
317
62
90
na
9.5 (9.4) mg/L
Day 0
9.6
NM
22.4
8.1
7.81
383
62
90
Day 1
22.8
7.7
6.49
384
Day 2
22.7
7.3
6.30
389
Day 3
22.6
7.6
6.67
393
Day 4
9.2
NM
22.7
7.6
6.78
395
64
90
9.4
19.0 (19.2) mg/L
Day 0
19.3
NM
22.5
8.1
7.79
466
62
90
19.8
Day 1
22.8
7.7
6.59
465
Day 2
22.6
7.3
7.09
470
Day 3
22.7
7.6
7.10
474
Day 4
19.1
NM
22.7
7.6
6.89
481
64
90
19.7
19.2
38.0 (38.8) mg/L
Day 0
38.9
NM
22.4
8.2
7.80
626
64
88
Day 1
22.8
7.7
6.25
623
Day 2
22.7
7.4
6.86
629
Day 3
22.7
7.6
6.78
633
Day 4
38.7
NM
22.7
7.6
6.75
639
66
90
38.8
76.1 (82.2) mg/L
Day 0
79.2
NM
22.3
8.2
7.82
941
72
88
Day 1
22.8
7.6
5.82
941
Day 2
22.6
7.5
6.81
948
Day 3
22.7
7.6
6.26
958
Day 4
85.3
NM
22.7
7.6
6.04
971
76
90
82.2
152.2(157.7) mg/L
Day 0
153.5
NM
22.4
8.2
7.82
1562
96
88
Day 1
22.8
7.7
7.03
1558
Day 2
22.7
7.5
6.02
1574
Day 3
22.7
7.6
6.09
1577
Day 4
162.1
NM
22.8
7.5
6.09
1587
110
90
157.7
304.4 (296.1) mg/L
Day 0
293.1
NM
22.5
8.2
7.82
2750
120
88
Day 1
22.8
7.7
7.03
2740
Day 2
22.7
7.5
5.46
2750
Day 3
22.6
7.6
5.76
2750
Day 4
299.2
NM
22.8
7.7
5.70
2740
126
90
296.1
a Nitrite Analysis Method 354.1 NM = not measured
b Nitrite + Nitrate Analysis Method 353.1 na = not applicable
60
-------
96-hr Toxicity of Nitrite on Hyalella azteca
The 96-hr test to determine the toxicity of nitrite on H. azteca was completed by GLEC.
H. azteca were collected from GLEC's laboratory culture. These organisms are
maintained in 10 gallon glass aquaria; plastic artificial turf and screen mesh serve as a
substrate for the culture. The tanks are filled with de-chlorinated Lake Michigan water
(City of Traverse City, Michigan water passed through an activated carbon filter).
Cultures are fed 50 mL of 4 g/L Tetrafin slurry daily. When visible algae are not
observed within the glass aquaria, algae {Selenastrum sp.) are used as a supplement to the
Tetrafin slurry. Additionally, on occasion, dried Aspen (Populus sp.) leaves are prepared
as a food supplement. The culture is maintained in a 16-hour light: 8-hour dark
photoperiod at a temperature between 23 and 26 °C.
Test organisms were acclimated to the dilution water (MHRW), test temperature and
other test conditions prior to test initiation. Once acclimated, test organisms were
examined for any disease, stress, parasites, etc. If free from ailments, test organisms were
randomly assigned to the test chambers (which were randomly assigned to testing
locations); four replicates were used per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 0.40, 0.67, 1.1, 1.8 and 3.0
mg N02-N/L.
Testing was conducted at 22 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 was determined using
the Probit method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 44; test results are provided in Table 45. Analytical chemistry data are provided
in Table 46. Accompanying information, including raw laboratory data, analytical
chemistry data, reference toxicant data and statistical analyses, is provided in Appendix
13.
61
-------
Table 44. Test conditions for 96-hour toxicity test on Hyalella azteca with nitrite.
Summary of Toxicity Test Conditions
l.
Test Species and Age:
Hyalella azteca, 10 days old, GLEC culture
2.
Test Type and Duration:
Static, 96 hours
3.
Test Dates:
August 6-August 10, 2009
4.
Test Temperature (°C):
22+1
5.
Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6.
Photoperiod:
16 h light, 8 h darkness
7.
Feeding Regime:
None
8.
Size of Test Vessel:
150 mL beaker
9.
Volume of Test Solutions:
100 mL
10.
No. of Test Organisms per Test Vessel:
5
11.
No. of Test Vessels per Treatment:
4
12.
Total No. of Test Organisms per Treatment:
20
13.
Target or Nominal Test Concentrations (mg NO2-
N/L):
3.0, 1.8, 1.1, 0.67 and 0.40
14.
Analytical Test Concentrations (average of samples
collected at test initiation and termination- mg N02-
N/L):
4.1,2.4, 1.4,0.81 and 0.54
15.
Renewal of Test Solutions:
None
16.
Dilution and Primary Control Water:
USEPA MHRW
17.
Test Material:
Sodium Nitrite: Sigma Aldrich, 97 +%, ACS Reagent Cas.
No. 7632-00-0, Batch # 08316DJ
18.
Secondary Control Water:
None
19.
Aeration:
None
20.
Endpoints Measured:
Mortality (LC50)
62
-------
Table 45. Test results for 96-hour toxicity test on Hyalella azteca with nitrite.
Results of a Hvalella arleca 96-Hour Static Acute Toxicity Test
Conducted 08/06/09 - 08/10/09 Using: Nitrite (Sodium Nitrite: Sisma Aldrich Cas. No. 7632-00-0)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
0
(0)
0
(0)
3.6 3.2 2.1 1.5
96-Hour LC50* = 1.5 mg/L
0.40 (0.54) mg/L
0
(0)
0
(0)
0
(0)
0
(0)
0.67 (0.81) mg/L
10
(10)
10
(10)
10
(10)
35
(35)
LC50 95% Confidence Limits
1.1 (1.4) mg/L
10
(10)
15
(15)
30
(30)
50
(50)
24-Hr
48-Hr
72-Hr
96-Hr
1.8 (2.4) mg/L
30
(30)
40
(40)
60
(60)
70
(70)
LL 2.7 2.4 1.7 1.2
UL 6.2 5.2 2.8 1.9
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
3.0 (4.1) mg/L
60
(65)
60
(65)
80
(80)
90
(90)
Method(s) Used to Determine LC50 Confidence
Limit Values: Probit
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
63
-------
Table 46. Analytical chemistry data for 96-hour toxicity test on Hyalella azteca with
nitrite.
Nominal (and Measu
Concentratio
red) Test
is
Nitrite3
(mg N02-N/L)
Nitrateb
(mg N03-N/L)
Temperature
(°C)
PH
(s.u.)
D.O.
(mg/L)
Conductivity
(mmhos)
Alkalinity
(mg/L)
Hardness
(mg/L)
Ammonia
(mg/L)
Dilution water/Control
Day 0
<0.0011
<0.0012
22.0
8.00
8.4
300
60
80
ND
Day 1
22.1
7.99
8.5
Day 2
23.2
7.77
8.3
Day 3
22.8
7.96
8.5
Day 4
0.02
0.044
22.1
7.80
8.0
290
60
80
0.02
0.056
0.02
0.40 (0.54) mg/L
Day 0
0.51
<0.0048
22.0
7.98
8.4
336
Day 1
22.2
7.97
8.5
Day 2
23.2
7.99
8.4
Day 3
22.8
7.98
8.5
Day 4
0.57
<0.0001
22.1
7.91
8.0
310
0.54
0.67(0.81) mg/L
Dav 0
0.87
<0.0072
22.0
7.99
8.4
340
<0.0072
Day 1
22.2
7.92
8.5
Day 2
23.1
8.01
8.4
Day 3
22.7
8.04
8.5
Day 4
0.78
0.055
22.1
7.90
8.0
315
0.79
0.81
1.1 (1.4) mg/L
Dav 0
1.44
<0.01
22.0
8.00
8.4
340
Day 1
22.2
7.95
8.5
Day 2
23.1
8.06
8.5
Day 3
22.8
8.06
8.6
Day 4
1.43
<0.01
22.2
7.90
8.0
325
144
1.8 (2.4) mg/L
Day 0
2.40
<0.02
22.0
8.04
8.4
341
Day 1
22.2
7.90
8.5
Dav 2
23.1
8.06
8.5
Day 3
22.8
8.04
8.6
Dav 4
2.47
<0.02
22.2
7.96
8.0
325
244
3.0 (4.1) mg/L
Day 0
3.96
<0.05
22.0
8.06
8.4
342
60
80
ND
Dav 1
22.2
7.90
8.5
Day 2
23.1
8.07
8.6
Dav 3
22.8
8.04
8.5
Dav 4
4.29
<0.05
22.2
7.97
8.0
322
58
80
4.1
a Nitrite Analysis Method SM 4500 N02
b Nitrate Analysis Method SM 4500 N03
ND Not Detect; below detection limit
64
-------
96-hr Toxicity of Nitrite on Pimeyhales yromelas
The 96-hr test to determine the toxicity of nitrite on P. promelas was completed by
GLEC. Test organisms, collected from the GLEC laboratory culture, were acclimated to
the dilution water (de-chlorinated Lake Michigan water), test temperature and other test
conditions prior to test initiation. Once acclimated, test organisms were examined for any
disease, stress, parasites, etc. If free from ailments, test organisms were randomly
assigned to the test chambers (which were randomly assigned to testing locations); two
replicates were used per treatment with ten organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 3.9, 6.6, 11,18 and 30 mg
N02-N/L.
Testing was conducted at 25 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Trimmed Spearman-Karber and Probit methods.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 47; test results are provided in Table 48. Analytical chemistry data are provided
in Table 49. Accompanying information, including raw laboratory data, analytical
chemistry data, reference toxicant data and statistical analyses, is provided in Appendix
14.
65
-------
Table 47. Test conditions for 96-hour toxicity test on Pimephales promelas with
nitrite.
Summary of Toxicity Test Conditions
l.
Test Species and Age:
Pimephales promelas, (weight 0.12g and 18 mm length),
GLEC culture
2.
Test Type and Duration:
Static, 96 hours
3.
Test Dates:
August 13-August 17, 2009
4.
Test Temperature (°C):
25+1
5.
Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6.
Photoperiod:
16 h light, 8 h darkness
7.
Feeding Regime:
None
8.
Size of Test Vessel:
4000 mL beaker
9.
Volume of Test Solutions:
3500 mL
10.
No. of Test Organisms per Test Vessel:
10
11.
No. of Test Vessels per Treatment:
2
12.
Total No. of Test Organisms per Treatment:
20
13.
Target or Nominal Test Concentrations (mg NO2-
N/L):
30,18, 11, 6.6, and 3.9
14.
Measured Test Concentrations (average of samples
collected at test initiation and termination- mg NO2-
N/L):
33,18. 11, 5.8, and 4.1
15.
Renewal of Test Solutions:
None
16.
Dilution and Primary Control Water:
De-chlorinated Lake Michigan Water
17.
Test Material:
Sodium Nitrite: Sigma Aldrich, 97 +%, ACS Reagent Cas.
No. 7632-00-0, Batch # 08316DJ
18.
Secondary Control Water:
None
19.
Aeration:
None
20.
Endpoints Measured:
Mortality (LC50)
66
-------
Table 48. Test results for 96-hour toxicity test on Pimephales promelas with nitrite.
Results of a Pimephales promelas 96-Hour Static Acute Toxicity Test
Conducted 08/13/09 - 08/17/09 Usins: Nitrite (Sodium Nitrite: Sisma Aldrich Cas. No. 7632-00-0)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
5
(5)
5
(5)
5
(5)
5
(5)
>33 >33 >33 27.0
96-Hour LCS0* = 27.0 mg/L
3.9 (4.1) mg/L
10
(10)
10
(10)
10
(10)
15
(15)
6.6 (5.8) mg/L
10
(10)
10
(10)
10
(10)
10
(10)
LC50 95% Confidence Limits
11 (11) mg/L
0
(0)
0
(0)
0
(0)
5
(15)
24-Hr
48-Hr
72-Hr
96-Hr
18 (18) mg/L
20
(20)
25
(25)
30
(30)
35
(45)
LL NA NA NA 19.7
ULNA NA NA 41.4
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
30 (33) mg/L
10
(25)
25
(25)
45
(65)
65
(65)
Method(s) Used to Determine LC50 Confidence
Limit: Trimmed Spearman-Karber
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
67
-------
Table 49. Analytical chemistry data for 96-hour toxicity test on Pimephales promelas
with nitrite.
Nominal (Measured)Test
Concentration
Temperature
PH
Conductivity Alkalinity Hardness Ammonia
Dilution
water/Control
Day 0
<0.0011
0.23
25.0
7.99
8.1
8.2
290
102
144
ND
0.23
Day 1
24.6
7.66
6.7
6.7
Day 2
24.2
7.55
6.1
5.8
Day 3
24.5
7.59
6.0
Day 4
0.01
0.26
24.7
7.67
6.0
6.1
304
100
136
ND
1.15
7.69
0.01
3.9(4.1) mg/L
Day 0
3.90
<0.11
25.0
8.06
8.2
8.2
320
Day 1
24.6
7.90
7.1
6.9
Day 2
24.2
7.60
5.9
5.9
Day 3
24.4
7.65
6.0
Dav 4
4.20
<0.11
24.7
7.74
5.9
5.9
333
4.26
7.77
4.12
6.6 (5.8) mg/L
Day 0
5.18
0.52
25.0
8.07
8.4
8.3
330
Day 1
24.4
7.79
6.9
6.9
Day 2
24.3
7.56
5.5
5.9
Day 3
24.6
7.69
6.1
Day 4
6.39
<0.11
24.7
7.80
5.9
6.0
354
5.78
7.80
11 (11) mg/L
Day 0
10.65
<0.25
25.0
8.08
8.5
8.3
360
Day 1
24.3
7.79
7.3
7.2
Day 2
24.2
7.63
6.5
6.1
Day 3
24.6
7.74
6.3
Day 4
10.65
<0.11
24.7
7.80
5.7
5.9
386
10.65
7.81
18 (18) mg/L
Dav 0
17.96
0.34
25.0
8.08
8.5
8.4
424
Day 1
24.3
7.79
7.3
7.3
Dav 2
24.3
7.63
6.0
6.1
Day 3
24.3
7.69
6.0
Dav 4
17.35
4.88
24.6
7.75
5.9
6.1
437
7.68
17.66
30 (33) mg/L
Day 0
31.66
<0.61
25.0
8.06
8.6
8.5
507
98
136
ND
Dav 1
24.3
7.77
7.5
7.4
Dav 2
24.2
7.61
6.3
6.5
Dav 3
24.3
7.63
5.5
Dav 4
33.79
<0.36
24.5
7.75
5.7
5.7
520
100
132
ND
32.72
7.77
a Nitrite Analysis Method SM 4500 N02
b Nitrate Analysis Method SM4500 N03
ND Not Detect; below detection limit
68
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32-day Toxicity of Nitrite on Pimeyhales yromelas
The 32-day test to determine the toxicity of nitrite on P. promelas was completed by
GLEC. The fish were continuously exposed for 32 days to five concentrations of nitrite
(nominal concentrations of 1.9, 3.8, 7.6, 15 and 30 mg NO2-N/L - dilution factor of 0.5)
and to a dilution water control using a continuous flow-through system (Benoit et al.
1982). The temperature-controlled test concentration solutions were supplied to each test
chamber via the continuous flow-through system at a rate of approximately four
turnovers a day. There were four replicate test chambers for each treatment. The flow
through test was conducted at 25 ± 1 °C with a photoperiod of 16 hr light and 8 hr
darkness (ambient laboratory light).
After test concentrations had achieved steady state in the flow through system, the test
was initiated with < 24 hour old fertilized embryos. The embryos were randomly
assigned to incubation cups until each incubation cup contained 30 embryos. The
incubation cups were randomly assigned to the 2.5 L glass test chambers (1 cup per
chamber) and suspended in the test solutions from a rocker arm assembly. The rocker
arm assembly moves the incubation cups in a reciprocal motion within each test chamber.
Embryos were inspected on a daily basis and the number of live, hatched and dead
embryos was recorded. On Day 5 of the test (three days after first hatch), the surviving
fish were randomly thinned to achieve 20 fish in each test chamber. The remainder of the
surviving fish was discarded. The number of surviving fish was recorded at test
termination (32 days). In addition, the wet weights were recorded for each fish at test
termination. Because of the size range of fish in each test chamber, all of the fish from
each test chamber were weighed together to determine average dry weight.
Instantaneous water temperature measurements made on August 11, 2009 (Day 4) ranged
from 25.5°C -26.3°C within individual replicates; this exceeded the allowed range of 25
± 1 °C in the toxicity testing method. However, the average water temperature (across the
duration of the test) in each replicate was always ± 0.3 °C of the target test temperature
(25 °C) in all treatments. In addition, the water temperature as recorded by the continuous
temperature logger was never outside the range outlined in the method. Therefore, the
water temperature exceedance likely had no effect on the results of this test.
In addition, on test day 24 there was an instance of low DO (5.8 mg/L) in replicate four
of the laboratory control. Low DO concentrations were never observed on any other day
of the test. Therefore, the one instance of low DO was an anomaly and likely had no
effect on the results of this test.
On August 27, 2009 (day 20) a diluter malfunction (stock solution metering pump)
occurred for 8 hours. Analytical chemistry samples were collected from replicate one of
each test concentration subsequent to this malfunction that analysis indicated that the test
solution concentrations were significantly less than the target concentrations. The
measured concentration of nitrite in the five test concentrations on day 20 was less than
50 percent of the time-weighted average measured concentration; the duration of the
malfunction was less than 10 percent (approximately 3.1 percent) of the total duration of
69
-------
the test. Therefore, the diluter malfunction likely had little, if any, affect on the results of
this test.
Once the test was complete, the LC50, NOEC and LOEC values were determined using
the average measured concentrations with the Spearman Karber and ANOVA methods
while LC25, LC2o, LCio values were determined with the Probit method. EC50, EC25j EC2o
and ECio values were estimated using EPA's TRAP.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 50; test results are provided in Table 51. Survival and growth data are provided
in Table 52 and analytical chemistry data are provided in Table 53. Accompanying
information, including raw laboratory data, analytical chemistry data and statistical
analyses, is provided in Appendix 15.
70
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Table 50. Test conditions for 32-day toxicity test on Pimephales promelas with
nitrite.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Pimephales promelas, (<24 hour fertilized embryos ),
GLEC culture
2. Test Type and Duration:
Continuous flow-through; 32 days
3. Test Dates:
August 7-September 8, 2009
4. Test Temperature (°C):
25+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
Live Brine Shrimp (Artemia nauplii) Twice daily
8. Size of Test Vessel:
2.5 Liter glass Tank
9. Volume of Test Solutions:
2000 mL
10. No. of Test Organisms per Test Vessel:
30 eggs, thinned to 20 larvae after hatch
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
120 eggs, thinned to 80 larvae after hatch
13. Target or Nominal Test Concentrations (mg NO2-
N/L):
30,15,7.6, 3.8, and 1.9
14. Analytical Test Concentrations (average of samples
collected at test initiation and termination- mg NO2-
N/L):
15. Renewal of Test Solutions:
16. Dilution and Primary Control Water:
17. Test Material:
30,15,6.8, 3.4, and 1.7
Continuous flow through, 4 turnovers per day
De-chlorinated Lake Michigan Water
Sodium Nitrite: Sigma Aldrich, 97 +%, ACS Reagent Cas.
No. 7632-00-0, Batch # 08316DJ
18. Secondary Control Water:
19. Aeration:
20. Endpoints Measured:
None
None
Survival (LC50, LC25 LC20 andLC10,NOEC andLOEC) and
Growth (EC5o,EC25)EC2o andEC10,NOEC and LOEC)
71
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Table 51. Test results for 32-day toxicity test on Pimephales promelas with nitrite.
Results of a Pimephales promelas 32-Dav Continuous Flow Chronic Toxicity Test
Conducted 08/03/09 - 09/08/09 Using: Nitrite (Sodium Nitrite: Sisma Aldrich Cas. No. 7632-00-0)
Test Solution Concentrations
Measured
Primary Control/
Dilution Water
Secondary
1.7 mg/L
3.4 mg/L
6.8 mg/L
15 mg/L
30 mg/L
Embryo Percent Hatch (%)
100
97.5
95.8
97.5
96.7
99.2
32-Day Mean Survival (%)
90.0
88.8
86.3
78.8
38.8a
0a
32-Day Average Biomass1 (mg )
6.02
6.11
6.49
6.17
2.95a
0a
Embryo Percent Hatch NOEC:
30.2 mg/L
32-Day LC20
5.7 mg/L (4.3 mg/L - 7.0 mg/L)
32-Day LC50*:
11.3 mg/L (9.7 mg/L-13.2 mg/L)
32-Day LC10
2.3 mg/L (0.6 mg/L - 4.0 mg/L)
32-Day Survival NOEC:
6.8 mg/L
32-Day EC50
14.6 mg/L (12.7 mg/L - 16.8 mg/L)
32-Day Survival LOEC:
15.0 mg/L
32-Day EC25
11.7 mg/L (6.7 mg/L -20.4 mg/L)
32-Day Growth NOEC:
6.8 mg/L
32-Day EC20
11.0 mg/L (5.6 mg/L -21.8 mg/L)
32-Day Growth LOEC:
15.0 mg/L
32-Day EC10
9.3 mg/L (3.3 mg/L -26.1 mg/L)
32-Day LC25:
7.0 mg/L (5.7 mg/L - 8.2 mg/L)
a: Significantly different from dilution water control (p< 0.05)
*: All LC, EC, NOEC and LOEC values are determined based on the average measured nitrite
concentration.
NOEC: No-Observed-Effect-Concentration
LOEC: Lowest-Observed-Effect-Concentration
Biomass: Biomass is the average dry weight of the four replicates calculated by the total dry weight of
surviving organisms divided by the initial number of organisms (20).
72
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Table 52. Survival and growth data for 32-day toxicity test on Pimephales promelas
with nitrite.
Nominal (and Measured) T est
Concentration
Number of
Eggs at Test
Initiation
Number of
Hatched
Larvae
Number of
Dead Eggs
Percent
Hatched
Larvae
Number of
Larvae at T est
Termination
Percent Survival
at Test
Termination*
Biomass1
(mg)
Dilution
water/Control
Replicate # 1
30
30
0
100.0
20
100.0
5.72
Replicate # 2
30
30
0
100.0
19
95.0
6.28
Replicate # 3
30
30
0
100.0
17
85.0
5.73
Replicate # 4
30
30
0
100.0
16
80.0
6.33
Average 100.0 90.0 6.02
1.9 (1.7) mg/L
Replicate # 1
30
29
1
96.7
16
80.0
6.86
Replicate # 2
30
30
0
100.0
17
85.0
5.69
Replicate # 3
30
30
0
100.0
18
90.0
5.77
Replicate # 4
30
28
2
93.3
20
100.0
6.12
Average 97.5 88.8 6.11
3.8 (3.4) mg/L
Replicate # 1
30
29
1
96.7
19
95.0
6.37
Replicate # 2
30
26
4
86.7
12
60.0
7.04
Replicate # 3
30
30
0
100.0
19
95.0
6.26
Replicate # 4
30
30
0
100.0
19
95.0
6.28
Average 95.8 86.3 6.49
7.6 (6.8) mg/L
Replicate # 1
30
28
2
93.3
16
80.0
6.55
Replicate # 2
30
29
1
96.7
16
80.0
6.28
Replicate # 3
30
30
0
100.0
19
95.0
5.51
Replicate # 4
30
30
0
100.0
12
60.0
6.34
Average 97.5 78.8 6.17
15 (15) mg/L
Replicate # 1
30
30
0
100.0
9
45.0
3.27
Replicate # 2
30
28
2
93.3
2
10.0
0.88
Replicate # 3
30
29
1
96.7
9
45.0
3.43
Replicate # 4
30
29
1
96.7
11
55.0
4.22
Average 96.7 38.8 2.95
30 (30) mg/L
Replicate # 1
30
30
0
100.0
0
0.0
0.00
Replicate # 2
30
29
1
96.7
0
0.0
0.00
Replicate # 3
30
30
0
100.0
0
0.0
0.00
Replicate # 4
30
30
0
100.0
0
0.0
0.00
Average 99.2 0.0 0.00
* On Day 5 of the test, (three days after first hatch) the surviving fish were randomly thinned to 20 fish in each test chamber.
Percent surval at test termination is the number of surviving at test termination divided by 20.
1 Biomass: Biomass is the total dry weight of surviving organisms divided by the initial number of organisms (20)
73
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Table 53. Analytical chemistry data for 32-day toxicity test on Pimephales promelas
with nitrite.
Nominal (and Measured) Test
Conent rations
NO*
(mg N02-N/L)
NOj
(mg N03-N/L)
Temperature
(°C)
pH
(s.U.)
DO
(mg/L)
SC
(mmos)
Hardness
(mg/L)
Alkalinity
(mg/L)
Ammonia
(mg/L)
Dilution
Water/Control
Average
Replicate # 1
0.00
(0.00-0.01)
0.25
24.8
(24.0-25.4)
8.03
7.7
(6.9-8.5)
298
118
105
ND
Replicate # 2
0.01
(0.01-0.01)
0.26
25.2
(24.3-26.3)
8.13
8.1
(7.7-9.1)
304
ND
Replicate # 3
0.00
(0.00-0.01)
0.26
25.2
(24.4-25.7)
8.17
8.0
(7.4-9.4)
ND
Replicate # 4
0.01
(0.00-0.03)
0.25
25.3
(24.2-26.2)
8.03
7.3
(5.8-8.2)
301
132
102
ND
0.01
1.9 mg/L
(1.7 mg/L)
Average
Replicate # 1
1.44
(0.70*-2.04)
0.22
24.9
(24.1-25.5)
8.18
7.8
(6.7-9.1)
315
146
103
ND
Replicate # 2
1.60
(0.88-1.95)
0.26
25.2
(24.0-26.0)
8.25
8.0
(7.7-8.3)
324
ND
Replicate # 3
1.99
(1.52-2.41)
0.11
25.1
(24.5-25.8)
8.17
7.9
(7.7-8.0)
ND
Replicate # 4
1.86
(1.46-2.47)
0.25
25.1
(24.1-26.0)
8.23
7.8
(6.7-9.1)
318
132
106
ND
1.7
3.8 mg/L
(3.4 mg/L)
Average
Replicate # 1
3.07
(1,40*-4.20)
0.26
24.9
(24.0-25.6)
8.22
7.9
(7.0-9.2)
328
140
105
ND
Replicate # 2
3.5
(3.01-4.47)
0.22
25.0
(24.1-26.1)
8.27
8.1
(7.7-9.2)
292
ND
Replicate # 3
3.65
(2.92-4.29)
0.32
25.1
(24.5-25.9)
8.10
7.8
(7.5-8.1)
ND
Replicate # 4
3.56
(3.04-4.17)
0.14
25.1
(24.2-25.9)
8.18
7.7
(6.9-8.2)
326
132
104
ND
3.4
7.6 mg/L
(6.8 mg/L)
Average
Replicate # 1
6.36
(2.83*-7.34)
0.41
25.1
(24.1-25.8)
8.18
7.6
(7.0-8.0)
359
136
105
ND
Replicate # 2
6.91
(5.78-7.85)
0.44
25.2
(24.2-25.9)
8.26
7.9
(7.3-9.1)
368
ND
Replicate # 3
7.06
(6.09-8.22)
0.28
25.1
(24.3-25.7)
8.18
7.8
(7.4-8.0)
ND
Replicate # 4
7.31
(6.09-8.13)
0.17
25.0
(24.3-26.1)
8.23
7.7
(6.4-9.1)
357
132
106
ND
6.8
15 mg/L
(15 mg/L)
Average
Replicate # 1
14.1
(4.63*-17.5)
0.81
25.0
(24.0-26.0)
8.21
7.9
(7.2-9.1)
417
144
104
ND
Replicate # 2
15.0
(12.8-18.5)
0.76
25.0
(24.2-25.8)
8.27
7.9
(7.6-8.3)
431
ND
Replicate # 3
15.9
(15.0-17.2)
1.17
25.0
(24.3-25.7)
8.19
7.7
(7.6-8.0)
ND
Replicate # 4
15.3
(14.3-16.6)
0.61
25.3
(24.5-26.1)
8.29
8.0
(7.0-9.2)
426
124
104
ND
15
30 mg/L
(30 mg/L)
Average
Replicate # 1
30.0
O0.7*-51.8)
3.16
24.6
(24.0-25.4)
8.24
8.0
(7.4-9.4)
528
136
103
ND
Replicate # 2
30.4
(25.0-34.4)
1.75
25.0
(24.3-26.3)
8.28
8.1
(7.5-9.0)
545
ND
Replicate # 3
29.7
(21.0-42.3)
4.74
25.0
(24.3-25.8)
8.25
8.1
(8.0-8.2)
ND
Replicate # 4
30.7
(28.6-32.6)
1.81
25.1
(24.5-26.2)
8.29
7.7
(7.1-8.3)
454
132
104
ND
30
DO: Dissolved Oxygen
SC: Specific Conductance
ND: Non Detect; below detection limit.
* August 20,2009 (day 20) a metering diluter malfunction occurred and analyitcal chemistry samples were collected.
74
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48-hr Toxicity of Nitrite on Ceriodayhnia dubia
The 48-hr test to determine the toxicity of nitrite on C. dubia was completed by GLEC.
Test organisms, < 24-hours old collected from the GLEC laboratory culture, were
acclimated to the dilution water (DMW), test temperature and other test conditions prior
to test initiation. Once acclimated, test organisms were examined for any disease, stress,
parasites, etc. If free from ailments, test organisms were randomly assigned to the test
chambers (which were randomly assigned to testing locations); four replicates were used
per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 0.99, 1.6, 2.7, 4.6, and 7.6 mg
N02-N/L.
Testing was conducted at 25 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Probit and Spearman Karber methods.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 54; test results are provided in Table 55. Analytical chemistry data are provided
in Table 56. Accompanying information, including raw laboratory data, analytical
chemistry data, reference toxicant data and statistical analyses, is provided in Appendix
16.
75
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Table 54. Test conditions for 48-hour toxicity test on Ceriodaphnia dubia with
nitrite.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Ceriodaphnia dubia, < 24 hours old, GLEC culture
2. Test Type and Duration:
Static, 48 hours
3. Test Dates:
August 21-August 23, 2009
4. Test Temperature (°C):
25+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
30 mL beaker
9. Volume Test Solutions:
15 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Target or Nominal Test Concentrations (NO2-N/L):
7.6,4.6,2.7, 1.6, and 0.99
14. Analytical Test Concentrations (average of samples
collected at test initiation and termination- NO2-N/L):
7.3,4.7,2.8, 1.6, and 0.99
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA DMW
17. Test Material:
Sodium Nitrite: Sigma Aldrich, 97 +%, ACS Reagent Cas.
No. 7632-00-0, Batch # 08316DJ
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
76
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Table 55. Test results for 48-hour toxicity test on Ceriodaphnia dubia with nitrite.
Results of a Ceriodavhnia dubia 48-Hour Static Acute Toxicity Test
Conducted 08/21/09 - 08/23/09 Using: Nitrite (Sodium Nitrite: Sisma Aldrich Cas. No. 7632-00-0)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
5.8 4.6 NA NA
48-Hour LC50* = 4.6 mg/L
1.0 (1.0) mg/L
0
(0)
0
(0)
1.6 (1.6) mg/L
0
(0)
0
(0)
LC50* 95% Confidence Limits
2.7 (2.8) mg/L
0
(0)
0
(0)
24-Hr
48-Hr
72-Hr
96-Hr
4.6 (4.7) mg/L
25
(25)
50
(50)
LL 5.1 4.1 NA NA
UL 6.6 5.1 NA NA
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
7.6 (7.3) mg/L
80
(80)
100
(100)
Method(s) Used to Determine LC50 Confidence
Limit Values: Spearman-Karber and Probit
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
77
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Table 56. Analytical chemistry data for 48-hour toxicity test on Ceriodaphnia dubia
with nitrite.
Nominal (and Meas
Concent rati
ured) Test
on
Nitrite3
(mg N02-N/L)
Nitrate"
(mg N03-N/L)
Temperature
(°C)
PH
(s.u.)
D.O.
(mg/L)
Conductivity
(mmos)
Alkalinity
(mg/L)
Hardness
(mg/L)
Ammonia
(mg/L)
Dilution
water/Control
Average
Day 0
<0.0011
0.31
25.0
7.98
7.8
189
76
96
ND
<0.0011
0.30
Day 1
24.6
8.40
8.8
Day 2
0.020
0.17
24.8
8.08
8.9
192
0.020
1.0 (1.0) mg/L
Average
Day 0
0.99
0.12
25.0
8.10
7.8
206
Day 1
24.7
8.32
8.8
Day 2
0.98
0.33
24.8
8.12
9.0
210
0.99
1.6 (1.6) mg/L
Average
Day 0
1.72
0.26
25.0
8.11
7.9
214
Day 1
24.7
8.24
8.8
Day 2
1.52
0.31
24.8
8.20
9.0
220
1.64
0.33
1.63
2.7 (2.8) mg/L
Average
Dav 0
2.98
0.05
25.0
8.12
8.0
223
Day 1
24.6
8.24
8.8
Dav 2
2.62
0.37
24.8
8.22
9.0
229
2.80
4.6 (4.7) mg/L
Average
Day 0
4.7
<0.18
25.0
8.13
8.0
236
Day 1
24.6
8.22
8.9
Day 2
4.7
0.41
24.8
8.30
9.0
234
4.7
7.6 (7.3) mg/L
Average
Day 0
7.6
<0.11
25.0
8.14
8.0
257
76
108
ND
Dav 1
24.6
8.21
9.0
Day 2
7.0
<0.11
24.8
8.36
9.0
246
7.3
a Nitrite Analysis Method SM 4500 N02
b Nitrate Analysis Method SM4500 N03
ND Not Detect; belcw detection limit
78
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7-day Toxicity of Nitrite on Ceriodayhnia dubia
The 7-day test to determine the toxicity of nitrite on C. dubia was completed by GLEC.
Test organisms, < 24-hours old collected from the GLEC laboratory culture, were
acclimated to the dilution water (DMW), test temperature and other test conditions prior
to test initiation. Once acclimated, test organisms were examined for any disease, stress,
parasites, etc. If free from ailments, test organisms were randomly assigned to the test
chambers (which were randomly assigned to testing locations); ten replicates were used
per treatment with one organism per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static-renewal conditions. Serial dilutions of the highest test
concentration (known weight of test chemical dissolved in a known volume of dilution
water) were made to prepare the following nominal test concentrations: 0.48, 0.95, 1.9,
3.8, and 7.6 mg N02-N/L.
Testing was conducted at 25 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). On a daily basis, organisms were fed Selenastrum
capricornutum and YTC (at renewal) and examined for mortality. As outlined in ASTM
Method E1295 (ASTM 2006 b), the duration of this test is based primarily on brood
production. Therefore, because greater than 60% of surviving control females had three
broods at Day 6 of the test, the test was terminated early and no results were reported for
Day 7.
Once the test was complete, the 48-hour and 6-day LC50 values and 6-day NOEC and
LOEC values were determined using the average measured concentrations with the
Spearman Karber and ANOVA methods. LC25,LC2o and LC10 values were determined
using the Probit method and EC50, EC25, EC20 and EC10 values were estimated using EPA's
TRAP.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 57; test results are provided in Table 58. Analytical chemistry data are provided
in Table 59. Accompanying information, including raw laboratory data, analytical
chemistry data, reference toxicant data and statistical analyses, is provided in Appendix
17.
79
-------
Table 57. Test conditions for 7-day toxicity test on Ceriodaphnia dubia with nitrite.
Summary of Toxicity Test Conditions
l.
Test Species and Age:
Ceriodaphnia dubia, < 24 hours old, GLEC culture
2.
Test Type and Duration:
Three Brood Static-Renewal
3.
Test Dates:
August 21-August 27, 2009
4.
Test Temperature (°C):
25+1
5.
Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6.
Photoperiod:
16 h light, 8 h darkness
7.
Feeding Regime:
Daily: Selenastrum capricornutum and YTC at renewal
8.
Size of Test Vessel:
30 mL beaker
9.
Volume Test Solutions:
15 mL
10.
No. of Test Organisms per Test Vessel:
1
11.
No. of Test Vessels per Treatment:
10
12.
Total No. of Test Organisms per Treatment:
10
13.
Target or Nominal Test Concentrations (mg NO2-
N/L):
7.6,3.8, 1.9, 0.95, and 0.48
14.
Analytical Test Concentrations (average of samples
collected at test initiation and termination- mg N02-
N/L):
7.3,3.6, 1.8, 0.82, and 0.36
15.
Renewal of Test Solutions:
Daily
16.
Dilution and Primary Control Water:
USEPA DMW
17.
Test Material:
Sodium Nitrite: Sigma Aldrich, 97 +%, ACS Reagent Cas.
No. 7632-00-0, Batch # 08316DJ
18.
Secondary Control Water:
Boardman River Culture Water
19.
Aeration:
None
20.
Endpoints Measured:
Survival (LC50, LC25)LC20 andLC10,NOEC andLOEC) and
Reproduction (EC50)EC25)EC2oandEC10,NOEC andLOEC)
80
-------
Table 58. Test results for 7-day toxicity test on Ceriodaphnia dubia with nitrite.
Results of a Ceriodavhnia dubia 7-Dav Static Renewal Chronic Toxicity Test
Conducted 08/21/09 - 08/27/09 Using: Nitrite (Sodium Nitrite: Sisma Aldrich Cas. No. 7632-00-0)
Nominal (Measured)
Concentrations
Primary Control/
Dilution Water
Secondary
Control
0.48 (0.36)
mg/L
0.95
(0.82)
mg/L
1.90 (1.75)
mg/L
3.81 (3.56)
mg/L
7.61
(7.31)
mg/L
48-hour Survival (%)
100
100
100
100
100
100
0
6-day Mean Survival (%)
100
100
100
90
100
90
0
6-day Mean
Reproduction/F emale
25.1
20.7
25.0
23.3
19.1
3.6
0
48-Hour LC50*
5.1 mg/L
6-Day LC20:
3.3 mg/L (2.3 mg/L -4.3 mg/L)
6-Day LC50*
4.7 mg/L (4.1 mg/L - 5.4 mg/L)
6-Day I. C1!
2.5 mg/L (1.4 mg/L - 3.6 mg/L)
6-Day Mean Survival (%) NOEC*
3.6 mg/L
6-Day EC50:
2.4 mg/L (2.1 mg/L -2.8 mg/L)
6-Day Mean Survival (%) LOEC*
7.3 mg/L
6-Day EC25:
1.9 mg/L (1.5 mg/L -2.3 mg/L)
6-Day Mean Reproduction per
Female NOEC*
0.82 mg/L
6-Day EC2o:
1.7 mg/L (1.4 mg/L - 2.2 mg/L)
6-Day Mean Reproduction per
Female LOEC*
1.8 mg/L
6-Day EC10:
1.4 mg/L (1.1 mg/L -1.9 mg/L)
6-Day LC25:
3.5 mg/L (2.5 mg/L - 4.6 mg/L)
All LC, EC, NOEC and LOEC values are determined based on the analytical chemistry results.
NOEC: No Observed Effect Concentration
LOEC: Lowest Observed Effect Concentration
81
-------
Table 59. Analytical chemistry data for 7-day toxicity test on Ceriodaphnia dubia
with nitrite.
Nominal (and Meas
Test Concentrat
ured)
on
Nitrite3
(mg N02-N/L)
Nitrate"
(mg N03-N/L)
Temperature
(°C)
old
PH
(S.U
newN
)
old°
D.O
(mg/
newN
L)
old°
Conductivity
(mmhos)
Alkalinity
(mg/L)
Hardness
(mg/L)
Ammonia
(mg/L)
Dilution water/Control
Average
Day 0™
<0.0011
0.307
24.7
8.00
7.9
183
76
96
ND
<0.0011
0.305
Day 1°
0.006
<0.0012
24.7
8.16
8.35
8.0
8.7
195
0.005
<0.0012
Day 2n
0.002
0.258
24.7
8.20
8.47
8.0
9.0
180
Day 3°
24.6
8.21
8.88
7.3
8.9
197
74
100
Day 4n
0.02
0.061
25.1
8.10
8.56
8.2
8.9
187
Day 5°
0.01
0.079
24.7
8.12
8.47
7.6
9.0
181
58
88
ND
0.074
Day 6°
0.02
<0.0012
8.31
8.9
<0.0012
0.01
0.48 (0.366) mg/L
Average
Day 0N
0.50
0.111
8.12
7.9
196
Dayl"
0.34
0.184
8.19
8.37
8.0
8.7
210
Day 2n
0.48
0.167
8.22
8.49
8.0
9.0
203
Day 3°
0.23
0.111
8.28
8.87
7.3
8.9
204
Day 4n
8.03
8.60
8.2
8.9
191
Day 5°
8.08
8.67
8.0
9.0
188
Day 6°
0.26
0.159
8.43
8.9
0.36
0.95(0.82) mg/L
Average
Day0N
1.00
0.239
8.16
8.0
206
Day 1u
0.80
0.255
8.20
8.37
8.0
8.7
217
Day 2n
8.23
8.50
8.0
9.0
218
Day 3°
8.29
8.87
7.3
8.9
208
Day 4n
0.85
0.357
8.05
8.64
8.2
8.9
199
Day 5°
0.74
0.172
8.10
8.64
7.8
9.3
194
Day 6°
0.72
0.251
8.44
8.9
0.82
1.90 (1.75) mg/L
Average
Day 0N
1.94
0.151
24.7
8.15
8.0
213
Day 1°
1.61
0.233
24.8
8.19
8.39
8.0
8.7
225
Day 2N
1.98
0.323
24.4
8.23
8.54
8.0
9.0
231
Day 3U
1.70
0.145
24.6
8.29
8.87
7.4
8.9
215
1.61
0.215
Day 4n
25.2
8.07
8.61
8.2
8.9
206
Day 5°
24.7
8.09
8.66
7.9
9.0
201
Day 6U
1.67
0.124
8.39
8.9
1.75
3.81 (3.56) mg/L
Average
Day0N
3.77
0.291
8.16
8.1
227
Day 1u
3.81
0.041
8.15
8.40
8.0
8.7
238
76
104
Day 2n
8.23
8.54
8.0
9.0
244
76
108
Day 3°
8.27
8.84
7.2
8.9
229
74
104
Day 4n
3.62
0.558
8.08
8.67
8.2
8.9
222
76
108
Day 5°
3.29
0.818
8.09
8.67
7.9
9.1
215
76
108
ND
Day 6°
3.38
0.332
8.43
8.9
3.57
7.61 (7.31) mg/L
Average
Day 0™
7.6
<0.11
8.14
8.4
253
76
108
ND
Day 1u
7.0
<0.11
8.17
8.40
8.5
8.7
261
76
104
Day 2n
Day 3°
Day 4n
Day 5°
Day 6°
7.3
a Nitrite Analysis Method SM 4500 N02
b Nitrate Analysis Method SM 4500 N03
ND Not Detect; below detection limit
N "New" test solutions are fresh solutions prepared each day and are used to initiate the toxicity test and to renew the existing test solutions.
82
-------
Boron
Table 60 provides a summary of estimated LC50 values for the nine toxicity tests
performed using boron. LC50 values ranged between 28.4 and >544 mg B/L.
Table 60. LC50 estimates for toxicity tests performed using boron.
Test Species and Duration
LC50 (mg B/L)
Lampsilis siliquoidea 96 hr
137
Pimephalespromelas - 96 hr
101
Pimephalespromelas - 32 day
28.4
Ceriodaphnia dubia - 48 hr (pH 7.75)
76.9
Pimephales promelas - 96 hr (pH 6.75)
70.6
Pimephales promelas - 96 hr (pH 7.75)
137
Pimephales promelas - 96 hr (pH 8.75)
133
Ligumia recta - 96 hr
147
Megalonaias nervosa - 96 hr
>544
For each of the acute toxicity tests completed using boron, two tables were generated: the
first summarizes the test results for each toxicity test, including nominal and analytical
test concentration and LC50 estimates with confidence intervals; the second table
summarizes analytical chemistry data collected throughout the toxicity tests. The results
of chronic tests performed with boron were summarized in three tables: the first
summarizes nominal and analytical test concentrations, LC50 estimates with confidence
intervals, NOEC and LOEC estimates, mean survival and mean biomass; the second table
summarizes replicate-specific survival and growth data and the third table summarizes
analytical chemistry data collected throughout the toxicity tests. Also discussed, if
applicable, are deviations from the guidance provided in the ASTM method used to
complete the toxicity testing.
83
-------
96-hr Toxicity of Boron on Lamysilis siliguoidea
The 96-hr test to determine the toxicity of boron on L. siliquoidea was completed by
INHS. Test organisms, < 5-day old juveniles collected from the Missouri State University
laboratory culture, were acclimated to the dilution water (MHRW), test temperature and
other test conditions prior to test initiation. Once acclimated, test organisms were
examined for any disease, stress, parasites, etc. If free from ailments, test organisms were
randomly assigned to the test chambers (which were randomly assigned to testing
locations); four replicates were used per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 500, 250, 125, 62.5, and 31.3
mg B/L.
Testing was conducted at 20 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 61; test results are provided in Table 62. Analytical chemistry data are provided
in Table 63. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 18.
84
-------
Table 61. Test conditions for 96-hour toxicity test on Lampsilis siliquoidea with
boron.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Lampsilis siliquoidea, juveniles <5 days old (Missouri State
University)
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
June 03 - 07,2009
4. Test Temperature (°C):
20+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
50 mL beaker
9. Volume of Test Solutions:
40 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg B/L):
500,250, 125, 62.5, and 31.3
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination-mg
B/L):
524, 260, 140, 72, and 34
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Boric acid: Acros Organics, 99.6%, ACS Reagent (crystals)
Cas. No. 10043-35-3, Lot # B0124654 and Borax (sodium
tetraborate decahydrate), 99.5 +% (for analysis ACS), Cas.
No. 1303-96-5, Lot # A0256722
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
85
-------
Table 62. Test results for 96-hour toxicity test on Lampsilis siliquoidea with boron.
Results of a Lampsilis siliauoidea 96-Hour Static Acute Toxicity Test
Conducted 06/03/09 - 06/07/09 Using: Boric acid Cas. No. 10043-35-3 and Borax Cas. No. 1303-96-5
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
5
5
5
5
>524 >524 181 137
96-Hour LCsn* = 137 mg/L
31.3 (34) mg/L
0
10
25
35
62.5 (72) mg/L
0
25
30
35
LC50 9 5% Confidence Limits
125 (140) mg/L
10
15
35
45
24-Hr
48-Hr
72-Hr
96-Hr
250 (260) mg/L
15
25
60
90
LLNR NR 110 86
ULNR NR 296 220
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
500 (524) mg/L
15
20
90
95
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
86
-------
Table 63. Analytical chemistry data for 96-hour toxicity test on Lampsilis siliquoidea
with boron.
Nominal (Measured) Test
Concentration Boron3 Temperature pH D.O. Conductivity Alkalinity Hardness
(mg/L) (°C) (s.u.) (mg/L) (jimhos) (mg/L) (mg/L)
Dilution water/Control
Day 0
0.1
20.0
8.0
7.81
305
60
90
Day 1
19.9
Day 2
20.0
Day 3
20.1
Day 4
1.1
19.9
8.0
7.10
305
62
90
0.3
31.3 (34) mg/L
Day 0
35
20.1
8.0
7.80
322
82
90
Day 1
19.8
Day 2
20.0
Day 3
20.1
Day 4
33
19.9
8.0
7.05
320
82
92
34
62.5 (72) mg/L
Day 0
69
20.0
8.0
7.80
344
90
90
Day 1
19.8
Day 2
20.1
Day 3
20.1
Day 4
76
20.0
8.0
6.99
350
92
92
72
125 (140) mg/L
Day 0
130
20.2
8.0
7.92
385
116
90
Day 1
19.7
Day 2
20.1
Day 3
20.1
Day 4
150
19.9
8.0
6.97
390
120
90
140
250 (260) mg/L
Day 0
250
20.0
8.0
7.96
464
164
88
Day 1
19.9
Day 2
20.1
Day 3
20.1
Day 4
270
19.9
8.0
6.92
465
164
90
260
500 (524) mg/L
Day 0
500
20.1
8.1
7.99
619
272
86
Day 1
20.0
Day 2
20.1
Day 3
20.1
Day 4
550
20.0
8.0
6.89
625
270
90
524
a Boron Analysis Method 200.7
87
-------
96-hr Toxicity of Boron on Meealonaias nervosa
The 96-hr test to determine the toxicity of boron onM nervosa was completed by INHS.
Test organisms, < 5-day old juveniles collected from the Genoa National Fish Hatchery,
were acclimated to the dilution water (MHRW), test temperature and other test conditions
prior to test initiation. Once acclimated, test organisms were examined for any disease,
stress, parasites, etc. If free from ailments, test organisms were randomly assigned to the
test chambers (which were randomly assigned to testing locations); four replicates were
used per treatment with five organisms per replicate. In one replicate of the 250 mg/L
treatment, a test organism was inadvertently crushed, but this was accounted for in the
LC50 calculation.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 500, 250, 125, 62.5, and 31.3
mg B/L.
Testing was conducted at 20 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 64; test results are provided in Table 65. Analytical chemistry data are provided
in Table 66. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 19.
88
-------
Table 64. Test conditions for 96-hour toxicity test on Megalonaias nervosa with
boron.
Summary of Toxicity Test Conditions
l.
Test Species and Age:
Megalonaias nervosa, juveniles <5 days old, Genoa
National Fish Hatchery
2.
Test Type and Duration:
Static, 96 hours
3.
Test Dates:
October 16 - 20,2009
4.
Test Temperature (°C):
20+1
5.
Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6.
Photoperiod:
16 h light, 8 h darkness
7.
Feeding Regime:
None
8.
Size of Test Vessel:
50 mL beaker
9.
Volume of Test Solutions:
40 mL
10.
No. of Test Organisms per Test Vessel:
5
11.
No. of Test Vessels per Treatment:
4
12.
Total No. of Test Organisms per Treatment:
20
13.
Test Concentrations (mg B/L):
500,250, 125, 62.5, and 31.3
14.
Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination-mg
B/L):
544, 275, 140, 74, and 37
15.
Renewal of Test Solutions:
None
16.
Dilution and Primary Control Water:
USEPA MHRW
17.
Test Material:
Boric acid: Acros Organics, 99.6%, ACS Reagent (crystals)
Cas. No. 10043-35-3, Lot # B0124654 and Borax (sodium
tetraborate decahydrate), 99.5 +% (for analysis ACS), Cas.
No. 1303-96-5, Lot # A0256722
18.
Secondary Control Water:
None
19.
Aeration:
None
20.
Endpoints Measured:
Mortality (LC50)
89
-------
Table 65. Test results for 96-hour toxicity test on Megalonaias nervosa with boron.
Results of a Meealonaias nervosa 96-Hour Static Acute Toxicity Test
Conducted 10/16/09 - 10/20/09 Using: Boric acid Cas. No. 10043-35-3 and Borax Cas. No. 1303-96-5
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
0
0
0
>544 >544 >544 >544
96-Hour LCsn* = >544mg/L
31.3 (37) mg/L
0
0
0
0
62.5 (74) mg/L
0
0
5
10
LC50 9 5% Confidence Limits
125 (140) mg/L
0
0
5
15
24-Hr
48-Hr
72-Hr
96-Hr
250 (275) mg/L
0
5
10
20
LL NR NR NR NR
ULNR NR NR NR
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
500 (544) mg/L
0
0
0
5
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
90
-------
Table 66. Analytical chemistry data for 96-hour toxicity test on Megalonaias nervosa
with boron.
Nominal (Measured) Test
Concentration
Boron"
Temperature
PH
D.O.
Conductivity
Alkalinity
Hardness
(mg/L)
(°C)
(S.U.)
(mg/L)
(mmos)
(mg/L)
(mg/L)
Day 0
<0.02
21.0
7.8
7.95
300
60
88
Day 1
20.8
7.7
8.27
303
Day 2
20.5
7.8
8.21
305
Day 3
20.3
8.0
8.21
290
Day 4
<0.02
20.6
8.0
7.62
337
68
90
Dilution water/Control
na
Day 0
36
21.0
8.0
8.21
320
70
88
Day 1
20.5
7.9
8.32
320
Day 2
20.5
7.9
8.22
320
Day 3
20.4
8.0
8.28
330
Day 4
38
20.6
8.0
8.15
351
72
88
31.3 (37) mg/L
37
Day 0
72
21.0
8.0
8.20
340
86
88
Day 1
20.8
7.9
8.34
343
Day 2
20.5
7.9
8.22
345
Day 3
20.3
8.0
8.27
347
Day 4
76
20.5
8.0
8.25
364
90
88
62.5 (74) mg/L
74
Day 0
140
21.0
7.9
8.25
381
110
88
Day 1
20.9
8.0
8.38
389
Day 2
20.6
8.0
8.23
390
Day 3
20.5
8.1
8.29
401
Day 4
140
20.7
8.1
8.27
417
115
88
125 (140) mg/L
140
Day 0
270
21.0
7.9
8.23
460
160
88
Day 1
20.8
8.0
8.40
461
Day 2
20.6
8.0
8.21
461
Day 3
20.5
8.1
8.25
488
Day 4
280
20.8
8.1
8.28
504
178
88
250 (275) mg/L
275
Day 0
520
21.0
7.9
8.24
613
266
86
Day 1
20.9
8.0
8.32
616
Day 2
20.6
8.0
8.20
618
Day 3
20.4
8.1
8.25
638
Day 4
570
20.8
8.2
8.23
654
276
88
500 (544) mg/L
544
3 Boron Analysis Method 200.7
na=not applicable
91
-------
96-hr Toxicity of Boron on Lisumia recta
The 96-hr test to determine the toxicity of boron on L. recta was completed by INHS.
Test organisms, < 5-day old juveniles collected from the Missouri State University
laboratory culture, were acclimated to the dilution water (MHRW), test temperature and
other test conditions prior to test initiation. Once acclimated, test organisms were
examined for any disease, stress, parasites, etc. If free from ailments, test organisms were
randomly assigned to the test chambers (which were randomly assigned to testing
locations); four replicates were used per treatment with five organisms per replicate. One
replicate was mistakenly loaded with only four individuals, but this was accounted for in
the LC50 calculation.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 500, 250, 125, 62.5, and 31.3
mg B/L.
Testing was conducted at 20 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 67; test results are provided in Table 68. Analytical chemistry data are provided
in Table 69. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 20.
92
-------
Table 67. Test conditions for 96-hour toxicity test on Ligumia recta with boron.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Ligumia recta, juveniles <5 days old, Missouri State
University
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
September 10 - 14, 2009
4. Test Temperature (°C):
20+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
50 mL beaker
9. Volume of Test Solutions:
40 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg B/L):
500,250, 125, 62.5, and 31.3
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination-mg
B/L):
510,255, 130, 64, and 33
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Boric acid: Acros Organics, 99.6%, ACS Reagent (crystals)
Cas. No. 10043-35-3, Lot # B0124654 and Borax (sodium
tetraborate decahydrate), 99.5 +% (for analysis ACS), Cas.
No. 1303-96-5, Lot # A0256722
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
93
-------
Table 68. Test results for 96-hour toxicity test on Ligumia recta with boron.
Results of a Lieumia recta 96-Hour Static Acute Toxicity Test
Conducted 09/10/09 - 09/14/09 Usins: Boric acid Cas. No. 10043-35-3 and Borax Cas. No. 1303-96-5
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
0
0
0
>510 >510 >510 147
96-Hour LCS„* = 147 mg/L
31.3 (33)mg/L
0
0
0
30
62.5 (64) mg/L
0
0
0
30
LC50 9 5% Confidence Limits
125 (130) mg/L
0
0
0
50
24-Hr
48-Hr
72-Hr
96-Hr
250 (255) mg/L
0
0
0
58
LLNR NR NR 88
UL NR NR NR 246
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
500 (510) mg/L
0
0
0
100
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
94
-------
Table 69. Analytical chemistry data for 96-hour toxicity test on Ligumia recta with
boron.
Nominal (Measured) Test
Concentration
Boron"
Temperature
PH
D.O.
Conductivity
Alkalinity
Hardness
(mg/L)
(°C)
(S.U.)
(mg/L)
(unihos)
(mg/L)
(mg/L)
Day 0
<0.02
20.4
7.9
8.18
301
60
92
Day 1
19.5
Day 2
19.1
Day 3
19.2
Day 4
<0.02
19.2
8.1
8.14
312
60
92
Dilution water/Control
na
Day 0
33
20.5
8.0
8.14
320
68
92
Day 1
19.5
Day 2
19.0
Day 3
19.3
Day 4
34
19.3
8.1
8.10
334
68
92
31.3 (33) mg/L
33
Day 0
62
20.5
8.0
8.13
341
90
90
Day 1
19.5
Day 2
19.0
Day 3
19.3
Day 4
66
19.3
8.1
8.11
353
90
90
62.5 (64) mg/L
64
Day 0
130
20.4
8.0
8.12
382
112
90
Day 1
19.5
Day 2
19.0
Day 3
19.3
Day 4
130
19.3
8.1
8.05
394
112
90
125 (130) mg/L
130
Day 0
250
20.5
8.1
8.12
460
170
90
Day 1
19.5
Day 2
19.2
Day 3
19.3
Day 4
260
19.3
8.1
8.03
472
170
90
250 (255) mg/L
255
Day 0
500
20.5
8.1
8.11
616
270
90
Day 1
19.5
Day 2
19.4
Day 3
19.1
Day 4
520
19.1
8.1
8.12
634
270
90
500 (510) mg/L
510
3 Boron Analysis Method 200.7
na = not applicable
95
-------
96-hr Toxicity of Boron on Pimeyhales yromelas
The 96-hr test to determine the toxicity of boron on P. promelas was completed by
GLEC. Test organisms, collected from the GLEC laboratory culture, were acclimated to
the dilution water (de-chlorinated Lake Michigan water), test temperature and other test
conditions prior to test initiation. Once acclimated, test organisms were examined for any
disease, stress, parasites, etc. If free from ailments, test organisms were randomly
assigned to the test chambers (which were randomly assigned to testing locations); two
replicates were used per treatment with ten organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 38.9, 64.8, 108, 180, 300 and
500 mg B/L.
Testing was conducted at 25 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Probit method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 70; test results are provided in Table 71. Analytical chemistry data are provided
in Table 72. Accompanying information, including raw laboratory data, analytical
chemistry data, reference toxicant data and statistical analyses, is provided in Appendix
21.
96
-------
Table 70. Test conditions for 96-hour toxicity test on Pimephales promelas with
boron.
Summary of Toxicity Test Conditions
l.
Test Species and Age:
Pimephales promelas, (weight 0.12g and 19.8 mm length),
GLEC Culture
2.
Test Type and Duration:
Static, 96 hours
3.
Test Dates:
September 2-September 6, 2009
4.
Test Temperature (°C):
25+1
5.
Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6.
Photoperiod:
16 h light, 8 h darkness
7.
Feeding Regime:
None
8.
Size of Test Vessel:
4000 mL beaker
9.
Volume of Test Solutions:
3500 mL
10.
No. of Test Organisms per Test Vessel:
10
11.
No. of Test Vessels per Treatment:
2
12.
Total No. of Test Organisms per Treatment:
20
13.
Target or Nominal Test Concentrations (mg B/L):
500, 300, 180, 108, 64.8, and 38.9
14.
Analytical Test Concentrations (average of samples
collected at test initiation and termination-mg B/L):
546,352, 200, 123, 71.5, and 46.1
15.
16.
17.
Renewal of Test Solutions:
Dilution and Primary Control Water:
Test Material:
None
De-chlorinated Lake Michigan Water
Boric Acid: Sigma Aldrich, ACS Reagent >= 99.5% Cas.
No. 10043-35-3, Batch 118K0007 and Borax (sodium
tetraborate decahydrate) Sigma Aldrich, > 99.5%, ACS
reagent, Cas. No. 1303-96-4, Lot# 118K0172
18.
Secondary Control Water:
None
19.
Aeration:
None
20.
Endpoints Measured:
Mortality (LC50)
97
-------
Table 71. Test results for 96-hour toxicity test on Pimepehales promelas with boron.
Results of a Pimevhales promelas 96-Hour Static Acute Toxicity Test
Conducted 09/02/09 - 09/06/09 Usins: Boron (Boric Acid: Sisma Aldrich Cas No. 10043 35-3)
(Borax: Sisma Aldrich Cas No. 1303-96-4)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50* Values (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
0
(0)
0
(0)
>546 312 173 101
96-Hour LCS0 *= 101 mg/L
38.9 (46.1) mg/L
0
(0)
0
(0)
0
(0)
0
(0)
64.8 (71.5) mg/L
0
(0)
0
(0)
0
(5)
15
(15)
LC50 *95% Confidence Limits
108 (123) mg/L
0
(0)
0
(0)
20
(20)
70
(70)
24-Hr
48-Hr
72-Hr
96-Hr
180 (200) mg/L
0
(0)
5
(5)
60
(60)
100
(100)
LL NA 271 150 88.3
ULNA 353 200 116
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
300 (352) mg/L
0
(0)
65
(65)
100
(100)
100
(100)
500 (546) mg/L
45
(45)
100
(100)
100
(100)
100
(100)
Method(s) Used to Determine LC50 Confidence
Limit Values: Probit
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
98
-------
Table 72. Analytical chemistry data for 96-hour toxicity test on Pimephales promelas
with boron.
Nominal (and M
Test Concent
easured)
rations
Boron
(mg/L)
Temperature
(°C)
pH
(s.u.)
D
(m
O.
g/L)
Conductivity
(mmos)
Alkalinity
(mg/L)
Hardness
(mg/L)
Ammonia
(mg/L)
Dilution
water/Control
Day 0
0.03
25.0
8.01
8.0
290
104
144
ND
Day 1
24.7
7.73
6.3
6.0
Day 2
24.8
7.54
5.9
6.1
Day 3
24.8
7.65
5.7
5.5
Day 4
0.04
24.7
7.95
6.0
6.1
278
100
132
0.035
38.9(46.1) mg/L
Day 0
54.7
25.0
8.16
8.0
318
Day 1
24.6
8.12
6.7
6.8
Day 2
24.8
8.02
6.4
6.5
Day 3
24.9
8.05
6.0
5.9
Day 4
37.4
24.7
8.07
6.4
6.5
323
46.1
64.8 (71.5) mg/L
Day 0
82
25.0
8.14
7.9
335
Day 1
24.7
8.17
7.7
6.7
Day 2
24.8
8.05
4.6
6.4
Day 3
24.8
8.11
6.3
4.5
Day 4
61
24.7
8.02
5.4
6.4
346
71.5
108(123) mg/L
Day 0
146
25.0
8.18
8.0
362
Day 1
24.8
8.18
7.0
6.4
Day 2
25.0
8.15
6.5
6.2
Day 3
24.9
8.13
5.9
6.4
Day 4
99.1
24.8
8.15
6.7
6.0
376
123
180(200) mg/L
Day 0
223
25.0
8.18
8.0
406
Day 1
25.1
8.17
6.2
6.3
Day 2
24.9
8.14
6.1
5.8
Day 3
24.9
8.11
5.9
4.7
Day 4
176
24.8
8.13
5.3
5.3
422
146
136
200
300 (352) mg/L
Day 0
388
25.0
8.17
8.0
476
Day 1
24.7
8.17
7.0
6.3
Day 2
24.8
8.17
6.8
5.4
Day 3
24.8
8.15
5.8
5.0
Day 4
316
352
500 (546) mg/L
Day 0
585
25.0
8.14
8.0
597
316
168
ND
Day 1
24.7
8.15
7.0
7.1
Day 2
24.8
8.13
7.4
7.5
Day 3
Day 4
507
546
a Boron Analysis EPA 200.8
ND Not Detect; below detection limit
99
-------
32-day Toxicity of Boron on Pimeyhales yromelas
The 32-day test to determine the toxicity of boron on P. promelas was completed by
GLEC. The fish were continuously exposed for 32 days to five concentrations of boron
(nominal concentrations of 6.3, 12.5, 25, 50 and 100 mg B/L) and to a dilution water
control using a continuous flow-through system (Benoit et al. 1982). The temperature-
controlled test concentration solutions were supplied to each test chamber via the
continuous flow-through system at a rate of approximately four turnovers a day. There
were four replicate test chambers for each treatment. The flow through test was
conducted at 25 ± 1 °C with a photoperiod of 16 hr light and 8 hr darkness (ambient
laboratory light).
After test concentrations had achieved steady state in the flow through system, the test
was initiated with < 24 hour old fertilized embryos. The embryos were randomly
assigned to incubation cups until each incubation cup contained 30 embryos. The
incubation cups were randomly assigned to the 2.5 L glass test chambers (1 cup per
chamber) and suspended in the test solutions from a rocker arm assembly. The rocker
arm assembly moves the incubation cups in a reciprocal motion within each test chamber.
Embryos were inspected on a daily basis and the number of live, hatched and dead
embryos was recorded. On Day 8 of the test (four days after first hatch), the surviving
fish were randomly thinned to achieve 20 fish in each test chamber. The remainder of the
surviving fish was discarded. The number of surviving fish was recorded at test
termination (32 days). In addition, the wet weights were recorded for each fish at test
termination. Because of the size range of fish in each test chamber, all of the fish from
each test chamber were weighed together to determine average dry weight.
Instantaneous water temperature measurements made on September 12 (Day 2: 23.5°C-
24.5°C), October 1 (Day 21: 23.7°C-24.3°C), October 9 (Day 29: 25.9°C-26.5°C),
October 11 (Day 31: 23.7°C-24.1°C), and October 12 (Day 32: 23.8°C-24.5°C) exceeded
the allowable range of 25 ± 1 °C in the toxicity testing method. However, the overall
average water temperatures (across the duration of the test) in each replicate were within
± 0.5 °C of the target test temperature (25 °C) in all treatments. Therefore, the water
temperature exceedances noted above likely had no effect on the results of this study.
On September 19 and 20, 2009 (test days 9 and 10) 60-90 percent mortality occurred in
replicates one and two of the laboratory control. It is of GLEC's opinion that the
equipment used during the thinning procedure on test day 8 contributed toxicity to these
two control replicates, resulting in the high fish mortality. This high mortality was
communicated to the EPA Work Assignment Manager and GLEC was advised to
continue the test with the assumption that further control mortality would result in test
failure. No further toxicity was observed in the remaining control fish throughout the test.
However, because of the mortality observed in the control treatments, only replicates
three and four of the laboratory control were used in the survival and growth statistical
comparisons.
100
-------
Once the test was complete, the LC50, NOEC and LOEC values were determined using
the average measured concentrations with the Spearman Karber and ANOVA methods.
LC25, LC20 and LC10 values were determined using the Probit method and EC50, EC25,
EC2o andECio values were estimated using EPA's TRAP.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 73; test results are provided in Table 74. Survival and growth data are provided
in Table 75 and analytical chemistry data are provided in Table 76. Accompanying
information, including raw laboratory data, analytical chemistry data and statistical
analyses, is provided in Appendix 22.
101
-------
Table 73. Test conditions for 32-day toxicity test on Pimephales promelas with
boron.
Summary of Toxicity Test Conditions
l.
Test Species and Age:
Pimephales promelas, (<24 hour fertilized embryos ),
GLEC Culture
2.
Test Type and Duration:
Continuous flow-through; 32 days
3.
Test Dates:
September 10-October 12, 2009
4.
Test Temperature (°C):
25+1
5.
Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6.
Photoperiod:
16 h light, 8 h darkness
7.
Feeding Regime:
Live Brine Shrimp (Artemia nauplii) Twice daily
8.
Size of Test Vessel:
2.5 Liter glass Tank
9.
Volume of Test Solutions:
2000 mL
10.
No. of Test Organisms per Test Vessel:
30 eggs, thinned to 20 larvae after hatch
11.
No. of Test Vessels per Treatment:
4
12.
Total No. of Test Organisms per Treatment:
120 eggs, thinned to 80 larvae after hatch
13.
Target or Nominal Test Concentrations (mg/L):
100, 50.0, 25.0, 12.5 and 6.25 mg/L-boron
14.
Analytical Test Concentrations (average of samples
collected at test initiation and termination-mg/L):
112, 56.5, 27.4,12.9, and 5.90 mg/L-nitrate
15.
Renewal of Test Solutions:
Continuous flow through, 4 turnovers per day
16.
Dilution and Primary Control Water:
De-Chlorinated Lake Michigan Water
17.
Test Material:
Boric Acid: Sigma Aldrich, ACS Reagent >= 99.5% Cas.
No. 10043-35-3, Batch 118K0007 and Borax (sodium
tetraborate decahydrate) Sigma Aldrich, > 99.5%, ACS
reagent, Cas. No. 1303-96-4, Lot# 118K0172
18.
Secondary Control Water:
None
19.
Aeration:
None
20.
Endpoints Measured:
Survival (LC50, LC25 LC20 andLC10,NOEC andLOEC) and
Growth (EC50)EC25)EC20 andEC10,NOEC and LOEC)
102
-------
Table 74. Test results for 32-day toxicity test on Pimepehales promelas with boron.
Results of a Pimevhales promelas
Conducted 09/10/09 - 10/12/09
32-Dav Continuous Flow Chronic Toxicity Test
Borax: Sisma Aldrich Cas. No. 1303-96-4)
Test Solution Concentrations
Measured
Primary 5.90 mg/L
12.9 mg/L
27.4 mg/L 56.5 mg/L
Control/
Dilution Water
Embryo Percent Hatch (%)
100 100
100
100 100
32-Day Mean Survival (%)
97.5 93.8
92.5
58.8a 1.3"
32-Day Average Biomass1 (mg )
10.1 7.27a
7.37 a
4.29a 0.06a
Embryo Percent Hatch NOEC:
100 mg/L
32-Day LC20
18.6 mg/L (15.9 mg/L
32-Day LC50*:
28.4 mg/L (25.5 mg/L-31.7 mg/L)
32-Day LC10
12.8 mg/L (9.6 mg/L -
32-Day Survival NOEC:
12.9 mg/L
32-Day EC50
28.7 mg/L (23.7 mg/L
32-Day Survival LOEC:
27.4 mg/L
32-Day EC25
25.1 mg/L (17.1 mg/L
32-Day Growth NOEC:
<5.9 mg/L
32-Day EC20
24.2 mg/L (14.3 mg/L
32-Day Growth LOEC:
5.9 mg/L
32-Day EC10
21.9 mg/L (8.6 mg/L -
32-Day LC25:
20.8 mg/L (18.2 mg/L - 23.4 mg/L)
*: All LC, EC, NOEC and LOEC values are determined based on the average measured boron concentration.
NOEC: No-Observed-Effect-Concentration
LOEC: Lowest-Observed-Effect-Concentration
112 mg/L
100
, -21.3 mg/L)
16.0 mg/L)
, - 34.6 mg/L)
, - 36.7 mg/L)
, -41.0 mg/L)
¦ 56.0 mg/L)
'Biomass: Biomass is the average dry weight of the four replicates calculated by the total dry weight of surviving
organisms divided by the initial number of organisms (20).
103
-------
Table 75. Survival and growth data for 32-day toxicity test on Pimephales promelas
with boron.
Nominal (and Measured) Test
Concentration
Number of
Eggs at Test
Initiation
Number of
Hatched
Larvae
Number of
Dead Eggs
Percent
Hatched
Larvae
Number of
Larvae at Test
Termination
Percent Survival
at Test
Termination*
Biomass1 (mg)
Dilution
water/Control
Replicate # 1
30
30
0
100.0
1
5.0
1.27
Replicate # 2
30
30
0
100.0
4
20.0
4.83
Replicate # 3
30
30
0
100.0
19
95.0
9.62
Replicate # 4
30
30
0
100.0
20
100.0
10.52
Average 100.0 97.5 2 10.07 2
6.25 (5.9) mg/L
Replicate # 1
30
30
0
100.0
17
85.0
6.69
Replicate # 2
30
30
0
100.0
18
90.0
6.84
Replicate # 3
30
30
0
100.0
20
100.0
7.34
Replicate # 4
30
30
0
100.0
20
100.0
8.21
Average 100.0 93.8 7.27
12.5 (12.9) mg/L
Replicate # 1
30
30
0
100.0
17
85.0
6.99
Replicate # 2
30
30
0
100.0
19
95.0
8.53
Replicate # 3
30
30
0
100.0
19
95.0
6.77
Replicate # 4
30
30
0
100.0
19
95.0
7.17
Average 100.0 92.5 7.37
25.0(27.4) mg/L
Replicate # 1
30
30
0
100.0
10
50.0
3.76
Replicate # 2
30
30
0
100.0
10
50.0
3.62
Replicate # 3
30
30
0
100.0
13
65.0
5.05
Replicate # 4
30
30
0
100.0
14
70.0
4.73
Average 100.0 58.8 4.29
50.0 (56.5) mg/L
Replicate # 1
30
30
0
100.0
0
0.0
0.00
Replicate # 2
30
30
0
100.0
0
0.0
0.00
Replicate # 3
30
30
0
100.0
1
5.0
0.25
Replicate # 4
30
30
0
100.0
0
0.0
0.00
Average 100.0 1.3 0.06
100 (112) mg/L
Replicate # 1
30
30
0
100.0
0
0.0
0.00
Replicate # 2
30
30
0
100.0
0
0.0
0.00
Replicate # 3
30
30
0
100.0
0
0.0
0.00
Replicate # 4
30
30
0
100.0
0
0.0
0.00
Average 100.0 0.0 0.00
* On Day 8 of the test, (four days after first hatch) the surviving fish were randomly thinned to 20 fish in each test chamber. Percent
surval at test termination is the number of surviving at test termination divided by 20.
1 Biomass: Biomass is the total dry weight of surviving organisms divided by the initial number of organisms (20)
2 Due to a technician error on day 8, only replicates 3 and 4 were used in the growth and survival analysis for the laboratory control
104
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Table 76. Analytical chemistry data for 32-day toxicity test on Pimephales promelas
with boron.
Nominal (and Measured) Test
Conentrations
Boron
(mg/L)
Temperature
<°C)
pH
(S.U.)
DO
(mg/L)
SC
(mmos)
Hardness
(mg/L)
Alkalinity
(mg/L)
Ammonia
(mg/L)
Dilution
Water/Control
Replicate #
1
0.0
(0.0-0.1)
25.1
(23.7-26.0)
8.08
7.5
(7.0-8.1)
290
112
100
ND
Replicate #
2
0.0
(0.0-0.1)
24.9
(23.8-26.3)
8.11
7.3
(6.8-7.8)
296
132
99
ND
Replicate #
3
0.1
(0.0-0.4)
24.9
(24.2-26.0)
8.16
7.2
(6.9-7.4)
300
NM
NM
ND
Replicate #
4
0.0
(0.0-0.1)
24.9
(24.1-25.5)
8.17
7.3
(7.1-7.5)
304
NM
NM
ND
Average Boron
mg/L 0
6.25 mg/L
(5.9 mg/L)
Replicate #
1
5.8
(4.9-6.6)
24.6
(23.9-25.6)
8.16
7.5
(7.0-7.9)
301
128
106
ND
Replicate #
2
6.0
(4.9-7.0)
24.9
(24.0-26.1)
8.17
7.3 (6.7
8.0)
305
132
104
ND
Replicate #
3
6.0
(5.0-7.1)
25.0
(24.1-26.2)
8.21
7.1
(6.6-7.5)
305
NM
NM
ND
Replicate #
4
5.9
(5.1-6.6)
24.8
(23.9-25.4)
8.18
7.1
(6.7-7.6)
309
NM
NM
ND
Average Boron
mg/L 5.9
12.5 mg/L
(12.9 mg/L)
Replicate #
1
13.0
(11.6-13.9)
24.8
(23.7-25.9)
8.17
7.4
(6.8-8.1)
307
132
110
ND
Replicate #
2
13.1
(11.5-14.2)
24.9
(24.1-26.1)
8.20
7.5
(6.9-8.0)
308
130
107
ND
Replicate #
3
13.1
(11.6-14.7)
25.1
(24.2-26.5)
8.25
7.3
(7.0-7.6)
310
NM
NM
ND
Replicate #
4
12.3
(11.0-13.8)
25.0
(24.2-25.6)
8.21
7.4
(7.1-7.8)
313
NM
NM
ND
Average Boron
mg/L 12.9
25.0 mg/L
(27.4 mg/L)
Replicate #
1
28.6
(23.8-34.3)
24.6
(23.7-25.7)
8.18
7.5
(7.1-8.0)
314
128
116
ND
Replicate #
2
28.0
(25.1-32.5)
24.7
(23.8-26.2)
8.21
7.5
(7.2-8.0)
316
128
113
ND
Replicate #
3
26.2
(22.7-31.1)
24.8
(23.8-26.1)
8.12
7.4
(7.1-7.8)
317
NM
NM
ND
Replicate #
4
26.3
(22.9-31.2)
24.5
(23.5-25.2)
8.22
7.4
(7.1-7.7)
320
NM
NM
ND
Average Boron
mg/L 27.4
50.0 mg/L
(56.5 mg/L)
Replicate #
1
56.1
(48.2-65.4)
24.8
(23.9-25.7)
8.21
7.6
(7.1-8.5)
331
132
128
ND
Replicate #
2
56.4
(46.9-63.3)
24.7
(24.1-26.0)
8.23
7.5
(7.2-8.1)
333
130
127
ND
Replicate #
3
57.4
(45.8-67.9)
24.8
(23.9-26.0)
8.27
7.5
(7.1-8.3)
351
NM
NM
ND
Replicate #
4
56.2
(45.3-63.4)
24.8
(23.9-25.4)
8.24
7.5
(7.3-8.0)
337
NM
NM
ND
Average Boron
mg/L 56.5
100 mg/L (112
mg/L)
Replicate #
1
111
(98.9-117)
24.8
(23.6-26.0)
8.22
7.6
(7.0-8.4)
363
128
152
ND
Replicate #
2
111
(93.7-117)
25.0
(23.9-26.2)
8.23
7.6
(7.2-8.4)
367
130
150
ND
Replicate #
3
111
(97.2-122)
25.0
(24.4-26.2)
8.27
7.4
(7.0-8.0)
367
NM
NM
ND
Replicate #
4
113
(103-123)
24.9
(24.0-25.6)
8.24
7.6
(7.3-8.0)
370
NM
NM
ND
Average Boron
ma/L 112
DO: Dissolved Oxygen ND: Non Detect; below detection limit.
SC: Specific Conductance NM: Not Measured
105
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48-hr Toxicity of Boron (yH 7.75) on Ceriodayhnia dubia
The 48-hr test to determine the toxicity of boron (pH 7.75) on C. dubia was completed by
GLEC. The C. dubia were continuously exposed for 48-hours to five concentrations of
boron and to a dilution water control with a target test pH of 7.75 (range of 7.65 to 7.85)
using a continuous flow-through system (modified Benoit mini-dilutor) and an in-line pH
adjustment/metering unit. The pH of both the dilution water and stock solution were
adjusted by using a 1:1 ratio of sulfuric acid and hydrochloric acid (acid solution). The
pH adjusted stock solution was delivered to mixing cells and diluted with pH adjusted,
de-chlorinated Lake Michigan water to achieve target nominal concentrations of boron
and a target test pH of 7.75.
Due to the buffering capacity of the borax and boric acid solution used to prepare the
boron concentrations, EPA agreed that GLEC should target the dilution water control pH
at 7.75 (range of 7.65 to 7.85). The five test concentrations were targeted to a pH of ± 0.1
pH unit from the pH value defined at test initiation in each test concentration (i.e.
regardless of whether or not the pH in the test concentrations were ± 0.1 pH units from
that observed in the control water). The temperature-controlled test concentration
solutions were supplied to each test chamber via the continuous flow-through system at a
rate of approximately four turnovers a day. There were four replicate test chambers for
each treatment. The flow through test was conducted at 25 ± 1 °C with a photoperiod of
16 hr light and 8 hr darkness (ambient laboratory light).
After test concentrations had achieved steady state in the flow through system, the test
was initiated with < 24 hour old C. dubia collected from the GLEC laboratory culture.
Although these organisms were cultured in, and acclimated to, the dilution water's
"natural" pH (typically between a pH of 7.9 and 8.2), they were not acclimated to the pH-
adjusted dilution water prior to test initiation for two reasons: 1) GLEC does not maintain
a laboratory culture of C. dubia in de-chlorinated Lake Michigan water maintained at a
pH of 7.75 (and this was outside the scope of work for this Work Assignment) and 2)
adequate acclimation of the organisms to the pH-adjusted dilution water would have
jeopardized the age requirement (< 24 hour old at test initiation) for test organisms
required under the toxicity testing method (ASTM 2007).
The C. dubia were randomly assigned to test cups until each test cup contained five C.
dubia. The test cups were randomly assigned to the 2.5 L glass test chambers (1 cup per
chamber) and suspended in the test solutions from a rod. C. dubia were counted on a
daily basis and the number of live C. dubia was recorded. pH was recorded twice a day,
at a minimum of eight hours apart (i.e. morning and evening). The number of surviving
C. dubia was recorded at test termination (48-hours).
The test was completed at the following nominal boron concentrations: 25.0, 50.0, 100,
200, and 400 mg/L. The average pH for the dilution water control measured in the C.
dubia toxicity test for the 48-hour test period was 7.77. The average pH over the 48-hour
test duration for the five test concentrations of 27.6, 49.8, 118, 223, and 391 mg/L was
7.92, 8.03, 8.03, 8.07, and 8.06, respectively.
106
-------
Once the test was complete, the LC50 was determined using the average measured test
concentrations with the Probit method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 77; test results are provided in Table 78. Analytical chemistry data are provided
in Table 79. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 23.
Table 77. Test conditions for 48-hour toxicity test on Ceriodaphnia dubia with boron
(pH 7.75).
Summary of Toxicity Test Conditions
1.
Test Species and Age:
Ceriodaphnia dubia, < 24 hours old, GLEC Culture
2.
Test Type and Duration:
Continuous flow-through, 48 hours
3.
Test Dates:
October 23-October 25, 2009
4.
Test Temperature (°C):
25+1
5.
Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6.
Photoperiod:
16 h light, 8 h darkness
7.
Feeding Regime:
None
8.
Size of Test Vessel:
2.5 Liter glass Tank
9.
Volume Test Solutions:
2000 mL
10.
No. of Test Organisms per Test Vessel:
10
11.
No. of Test Vessels per Treatment:
2
12.
Total No. of Test Organisms per Treatment:
20
13.
Target or Nominal Test Concentrations (mg/L):
400, 200, 100, 50.0, and 25.0
14.
Analytical Test Concentrations (average of samples
collected at test initiation and termination-mg/L):
391,223, 118, 49.8, and 27.6
15.
Renewal of Test Solutions:
Continuous flow through, 4 turnovers per day
16.
Dilution and Primary Control Water:
De-Chlorinated Lake Michigan Water
17.
Test Material:
Boric Acid: Sigma Aldrich, ACS Reagent >= 99.5% Cas.
No. 10043-35-3, Batch 118K0007 and Borax (sodium
tetraborate decahydrate) Sigma Aldrich, > 99.5%, ACS
reagent, Cas. No. 1303-96-4, Lot# 118K0172
18.
Secondary Control Water:
None
19.
Aeration:
None
20.
Endpoints Measured:
Mortality (LC50)
107
-------
Table 78. Test results for 48-hour toxicity test on Ceriodaphnia dubia with boron at
pH 7.75.
Results of a Ceriodavhnia dubia 48-Hour Static Acute Toxicity Test
Conducted 10/23/09 - 10/25/09 Using: Boron (Boric Acid: Sisma Aldrich Cas No. 10043-35-3)
(Borax: Sisma Aldrich Cas No. 1303-96-4)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
172 76.9 NA NA
48-Hour LCS0* = 76.9 mg/L
25.0 (27.6) mg/L
0
(0)
0
(0)
50.0 (49.8) mg/L
0
(0)
25
(25)
LC50* 95% Confidence Limits
100 (118) mg/L
15
(15)
75
(75)
24-Hr
48-Hr
72-Hr
96-Hr
200 (223) mg/L
75
(75)
100
(100)
LL 144 62.3 NA NA
UL 202 94.9 NA NA
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
400 (391) mg/L
100
(100)
100
(100)
Method(s) Used to Determine LC50 Confidence
Limit Values: Probit
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
108
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Table 79. Analytical chemistry data for 48-hour toxicity test on Ceriodaphnia dubia
with boron at pH 7.75.
Nominal (and Measured) Test
Conent rations
Boron
(mg/L)
Temperature
(°C)
pH
(s.U.)
DO
(mg/L)
SC
(mmos)
Hardness
(mg/L)
Alkalinity
(mg/L)
Ammonia
(mg/L)
Dilution
Water/Control
Average
Replicate #
1
0.05
(0.04-0.05)
24.6
(24.5-24.6)
7.65
(7.53-7.75)
7.7
(6.5-8.7)
320
146
47
ND
Replicate #
2
0.06
(0.04-0.07)
25.0
(24.9-25.1)
7.75
(7.70-7.82)
7.9
(6.9-8.6)
328
Replicate #
3
0.05
(0.03-0.07)
25.2
(25.1-25.2)
7.84
(7.77-7.94)
7.7
(7.1-8.1)
322
Replicate #
4
0.06
(0.05-0.07)
24.9
(24.9-24.9)
7.71
(7.56-7.79)
7.5
(6.4-8.1)
0.05
24.8
7.77
7.7
323
146
47
ND
25 nig/L
(27.6 mg/L)
Average
Replicate #
1
27.9
(27.2-28.5)
24.7
(24.5-24.8)
7.89
(7.80-7.95)
7.3
(5.9-8.1)
346
138
50
ND
Replicate #
2
27.3
(25.9-28.0)
25.1
(25.0-25.2)
7.96
(7.89-8.06)
7.7
(7.2-8.0)
350
Replicate #
3
27.4
(27.1-27.8)
24.8
(24.7-24.9)
7.92
(7.87-8.00)
7.6
(6.7-8.1)
Replicate #
4
28.0
(27.2-28.9)
24.8
(24.8-24.9)
7.97
(7.89-8.06)
7.7
(7.1-8.1)
27.6
24.7
7.92
7.6
348
138
50
ND
50 mg/L
(49.8 mg/L)
Average
Replicate #
1
49.8
(47.4-52.9)
24.6
(24.5-24.7)
8.00
(7.96-8.07)
7.4
(6.4-7.9)
364
144
57
ND
Replicate #
2
50.1
(47.8-53.7)
24.8
(24.7-24.8)
8.04
(7.97-8.11)
7.7
(7.1-8.0)
365
Replicate #
3
49.3
(47.5-52.7)
24.8
(24.7-24.9)
8.00
(7.96-8.07)
7.8
(7.3-8.1)
Replicate #
4
49.8
(47.8-53.5)
24.8
(24.7-24.9)
8.04
(7.97-8.11)
7.5
(6.5-8.1)
49.8
24.6
8.03
7.6
364
144
57
ND
100 mg/L
(118 mg/L)
Replicate #
1
120
(111-127)
24.8
(24.6-24.9)
8.07
(8.03-8.13)
7.5
(6.7-8.0)
411
128
77
ND
Replicate #
2
120
(112-124)
24.9
(24.8-25.0)
8.08
(8.02-8.14)
7.6
(6.8-8.0)
412
Replicate #
3
118
(110-130)
24.8
(24.7-24.9)
8.07
(8.02-8.13)
7.5
(6.4-8.1)
Replicate #
4
115
(105-125)
24.9
(24.6-25.1)
8.07
(8.00-8.14)
7.6
(6.9-8.0)
Average
118
24.8
8.03
7.5
411
128
77
ND
200 mg/L
(223 mg/L)
Replicate #
1
227
(221-232)
24.7
(24.6-24.8)
8.08
(8.02-8.15)
7.7
(7.2-8.0)
475
132
105
ND
Replicate #
2
222
(216-228)
24.5
(24.4-24.7)
8.08
(8.03-8.15)
7.5
(6.6-8.0)
477
Replicate #
3
218
(211-224)
24.7
(24.6-24.8)
8.09
(8.02-8.15)
7.7
(7.1-8.0)
Replicate #
4
225
(222-228)
25.1
(24.8-25.3)
8.07
(8.01-8.14)
7.6
(6.7-8.1)
Average
223
24.8
8.07
7.6
476
132
105
ND
400 mg/L
(391 mg/L)
Replicate #
1
392
(381-399)
24.5
(24.4-24.6)
8.06
(8.00-8.13)
7.6
(6.7-8.0)
587
132
167
ND
Replicate #
2
391
(386-396)
24.6
(24.4-24.8)
8.07
(8.01-8.14)
7.7
(7.1-8.0)
589
Replicate #
3
391
(388-394)
24.5
(24.3-24.8)
8.07
(8.01-8.14)
7.7
(7.2-8.0)
Replicate #
4
391
(384-401)
24.6
(24.4-24.7)
8.06
(8.01-8.13)
7.5
(6.4-8.0)
Average
391
24.6
8.06
7.6
588
132
167
ND
DO: Dissolved Oxygen ND: Non Detect; below detection limit.
SC: Specific Conductance NM: Not Measured
109
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96-hour Toxicity of Boron (yH 6.75) on Pimeyhales yromelas
The 96-hr test to determine the toxicity of boron (pH 6.75) on P. promelas was
completed by GLEC. The P. promelas (collected from the GLEC laboratory culture)
were continuously exposed for 96-hours to five concentrations of boron and to a dilution
water control with a target test pH of 6.75 (range of 6.65 to 6.85) using a continuous
flow-through system (modified Benoit mini-dilutor) and an in-line pH adjustment/
metering unit. The pH of both the dilution water and stock solution were adjusted by
using a 1:1 ratio sulfuric acid and hydrochloric acid (acid solution). The pH adjusted
stock solution was delivered to mixing cells and diluted with pH adjusted Lake Michigan
water to achieve target nominal concentrations of boron and a target test pH of 6.75. Due
to the buffering capacity of the borax and boric acid solution used to prepare the boron
concentrations, EPA agreed that GLEC should target to a pH of ± 0.1 pH unit from the
pH value defined at test initiation in each test concentration (i.e. regardless of whether or
not the pH in the test concentrations were ± 0.1 pH unit from that observed in the control
water). The temperature-controlled test concentration solutions were supplied to each test
chamber via the continuous flow-through system at a rate of approximately four
turnovers a day. There were two replicate test chambers for each treatment. The flow
through test was conducted at 25 ± 1 °C with a photoperiod of 16 hr light and 8 hr
darkness (ambient laboratory light).
After test concentrations had achieved steady state in the flow through system, the test
was initiated with the P. promelas. Although these organisms were cultured in, and
acclimated to, the dilution water's "natural" pH (typically between a pH of 7.9 and 8.2),
they were not acclimated to the pH-adjusted dilution water prior to test initiation for two
reasons: 1) GLEC does not maintain a laboratory culture of P. promelas in de-
chlorinated Lake Michigan water maintained at a pH of 6.75 (and this was outside the
scope of work for this Work Assignment) and 2) adequate acclimation of the organisms
to the pH-adjusted dilution water may have jeopardized the weight requirement (0.1-5 g
at test initiation) for test organisms required under the toxicity testing method (ASTM
2007).
The P. promelas were randomly assigned to the 2.5 L glass test chambers until each test
chamber contained ten P. promelas. P. promelas were counted on a daily basis and the
number of live P. promelas was recorded. pH was recorded twice a day, at a minimum of
eight hours apart (i.e., morning and evening). The number of surviving P. promelas was
recorded at test termination (96-hours).
The test was completed at the following nominal boron concentrations: 25.0, 50.0, 100,
200, and 400 mg/L (dilution factor of 0.5). The average pH for the dilution water control
measured in the P. promelas toxicity test for the 96-hour test period was 6.67. The
average pH over the 96-hour test duration for the five test concentrations of 32.9, 55.2,
122, 224, and 394 mg/L was 6.85, 6.93, 7.18, 7.28, and 7.33, respectively.
The hourly water temperatures as recorded by the continuous temperature logger did
show that test temperatures fell outside the temperature allowance in the early morning
110
-------
on October 29, 2009 (readings of 23.8 and 23.9 °C). However, instantaneous water
temperatures measured by GLEC technicians on October 28 and later in the morning on
October 29 never fell outside the acceptable range as outlined in the method. Therefore,
these water temperature exceedances were very brief and likely had no effect on the
results of this study.
Once the test was complete, the LC50 was determined using the average measured test
concentrations with the Probit and Spearman method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 80; test results are provided in Table 81. Analytical chemistry data are provided
in Table 82. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 24.
Ill
-------
Table 80. Test conditions for 96-hour toxicity test on Pimephales promelas with
boron (pH 6.75).
Summary of Toxicity Test Conditions
l.
Test Species and Age:
Pimephales promelas, (weight 0.1 lg andl8.4 mm length),
GLEC Culture
2.
Test Type and Duration:
Continuous flow-through, 96 hours
3.
Test Dates:
October 29-November 02,2009
4.
Test Temperature (°C):
25+1
5.
Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6.
Photoperiod:
16 h light, 8 h darkness
7.
Feeding Regime:
None
8.
Size of Test Vessel:
2.5 Liter glass Tank
9.
Volume of Test Solutions:
2000 mL
10.
No. of Test Organisms per Test Vessel:
10
11.
No. of Test Vessels per Treatment:
2
12.
Total No. of Test Organisms per Treatment:
20
13.
Target or Nominal Test Concentrations (mg/L):
400, 200, 100, 50.0, and 25.0
14.
Analytical Test Concentrations (average of samples
collected at test initiation and termination-mg/L):
394, 224, 122, 55.2, and 32.9
15.
16.
17.
Renewal of Test Solutions:
Dilution and Primary Control Water:
Test Material:
Continuous flow through, 4 turnovers per day
De-chlorinated Lake Michigan Water
Boric Acid: Sigma Aldrich, ACS Reagent >= 99.5% Cas.
No. 10043-35-3, Batch 118K0007 and Borax (sodium
tetraborate decahydrate) Sigma Aldrich, > 99.5%, ACS
reagent, Cas. No. 1303-96-4, Lot# 118K0172
18.
Secondary Control Water:
None
19.
Aeration:
None
20.
Endpoints Measured:
Mortality (LC50)
112
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Table 81. Test results for 96-hour toxicity test on Pimephales promelas with boron at
pH 6.75.
Results of a Pimephales promelas 96-Hour Static Acute Toxicity Test
Conducted 10/29/09 - 11/02/09 Using: Boron (Boric Acid: Sisma Aldrich Cas No. 10043-35-3)
fBorax: Sisma Aldrich Cas No. 1303-96-4)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50* Values (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
0
(0)
0
(0)
>394 297 163 70.6
96-Hour LCS0 *= 70.6 mg/L
25.0 (32.9) mg/L
5
(5)
5
(5)
10
(10)
10
(10)
50.0 (55.2) mg/L
5
(5)
5
(5)
5
(5)
20
(20)
LC50 *95% Confidence Limits
100 (122) mg/L
0
(0)
0
(0)
30
(30)
90
(90)
24-Hr
48-Hr
72-Hr
96-Hr
200 (224) mg/L
5
(5)
5
(5)
65
(65)
100
(100)
LL NA 285 130 58.3
UL NA 310 205 86.3
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
400 (394) mg/L
10
(10)
95
(95)
100
(100)
100
(100)
Method(s) Used to Determine LC50 Confidence
Limit Values: Probit and Spearman
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
113
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Table 82. Analytical chemistry data for 96-hour toxicity test on Pimephales promelas
with boron at pH 6.75.
Nominal (and Measured) Test
Conent rations
Boron
(mg/L)
T emperature
(°C)
PH
(S.U.)
DO
(mg/L)
SC
(mmos)
Hardness
(mg/L)
Alkalinity
(mg/L)
Ammonia
(mg/L)
Dilution
Water/Control
Average
Replicate #
1
0.04
(0.04-0.04)
24.4
(24.0-24.8)
6.62
(6.48-6.69)
7.3
(6.7-8.0)
327
130
70
ND
Replicate #
4
0.06
(0.05-0.06)
24.5
(24.0-24.8)
6.73
(6.63-6.88)
7.2
(6.7-8.3)
343
0.05
24.5
6.67
7.3
332
130
7
ND
25 n^/L
(32.9 mg/L)
Average
Replicate #
1
32.8
(30.3-38.6)
24.5
(24.1-24.9)
6.82
(6.73-6.93)
7.2
(6.6-8.0)
362
130
10
ND
Replicate #
3
33.1
(30.4-39.0)
24.6
(24.1-25.0)
6.88
(6.80-6.98)
7.6 (7.2
8.2)
365
32.9
24.5
6.85
7.4
363
130
10
ND
50 n^/L
(55.2 mg/L)
Average
Replicate #
1
55.2
(51.8-62.5)
24.4
(24.0-24.6)
6.94
(6.86-7.04)
7.4
(6.8-8.0)
382
126
11
ND
Replicate #
3
55.2
(51.1-61.1)
24.6
(24.3-24.8)
6.93
(6.83-7.04)
7.3
(6.5-8.1)
351
55.2
24.5
6.93
7.4
371
126
11
ND
100 n^/L
(122 mg/L)
Replicate #
1
120
(112-126)
24.5
(24.0-25.1)
7.17
(7.11-7.25)
7.4
(6.8-8.1)
440
130
18
ND
Replicate #
3
123
(119-129)
24.5
(24.1-24.9)
7.19
(7.13-7.26)
7.6
(7.1-8.3)
447
Average
122
24.5
7.18
7.5
442
130
18
ND
200 n^/L
(224 mg/L)
Replicate #
2
223
(219-234)
24.3
(24.0-24.9)
7.28
(7.22-7.35)
7.2
(6.5-8.2)
518
128
25
ND
Replicate #
4
224
(217-232)
25.3
(25.1-25.5)
7.28
(7.20-7.35)
7.3
(6.8-8.2)
527
Average
224
24.8
7.28
7.2
521
128
25
ND
400 mg/L
(394 mg/L)
Replicate #
1
391
(372-407)
24.3
(24.0-24.6)
7.33
(7.26-7.38)
7.4
(6.9-8.1)
670
126
41
ND
Replicate #
4
397
(368-426)
24.6
(24.3-25.1)
7.32
(7.24-7.37)
7.5
(7.1-8.2)
696
Average
394
24.4
7.33
7.4
678
126
41
ND
DO: Dissolved Oxygen ND: Non Detect; below detection limit.
SC: Specific Conductance NM: Not Measured
114
-------
96-hr Toxicity of Boron (yH 7.75) on Pimeyhales yromelas
The 96-hr test to determine the toxicity of boron (pH 7.75) on P. promelas was
completed by GLEC. The P. promelas (collected from the GLEC laboratory culture)
were continuously exposed for 96-hours to five concentrations of boron and to a dilution
water control with a target test pH of 7.75 (range of 7.65 to 7.85) using a continuous
flow-through system (modified Benoit mini-dilutor) and an in-line pH
adjustment/metering unit. The pH of both the dilution water and stock solution were
adjusted by using a 1:1 ratio of sulfuric acid and hydrochloric acid (acid solution). The
pH adjusted stock solution was delivered to mixing cells and diluted with pH adjusted
Lake Michigan water to achieve target nominal concentrations of boron and a target test
pH of 7.75. Due to the buffering capacity of the borax and boric acid solution used to
prepare the boron concentrations, EPA agreed that GLEC should target the dilution water
control pH at 7.75 (range of 7.65 to 7.85). The five test concentrations were targeted to a
pH of ± 0.1 pH unit from the pH value defined at test initiation in each test concentration
(i.e. regardless of whether or not the pH in the test concentrations were ±0.1 pH unit
from that observed in the control water). The temperature-controlled test concentration
solutions were supplied to each test chamber via the continuous flow-through system at a
rate of approximately four turnovers a day. There were two replicate test chambers for
each treatment. The flow through test was conducted at 25 ± 1 °C with a photoperiod of
16 hr light and 8 hr darkness (ambient laboratory light).
After test concentrations had achieved steady state in the flow through system, the test
was initiated with the P. promelas. Although these organisms were cultured in, and
acclimated to, the dilution water's "natural" pH (typically between a pH of 7.9 and 8.2),
they were not acclimated to the pH-adjusted dilution water prior to test initiation for two
reasons: 1) GLEC does not maintain a laboratory culture of P. promelas in de-
chlorinated Lake Michigan water maintained at a pH of 7.75 (and this was outside the
scope of work for this Work Assignment) and 2) adequate acclimation of the organisms
to the pH-adjusted dilution water may have jeopardized the weight requirement (0.1-5 g
at test initiation) for test organisms required under the toxicity testing method (ASTM
2007).
The P. promelas were randomly assigned to the 2.5 L glass test chambers until each test
chamber contained ten P. promelas. P. promelas were counted on a daily basis and the
number of live P. promelas was recorded. pH was recorded twice a day, at a minimum of
eight hours apart (i.e. morning and evening). The number of surviving P. promelas was
recorded at test termination (96-hours).
The test was completed at the following nominal boron concentrations: 25.0, 50.0, 100,
200, and 400 mg/L (dilution factor of 0.5). The average pH for the dilution water control
measured in the P. promelas toxicity test for the 96-hour test period was 7.68. The
average pH over the 96-hour test duration for the five test concentrations of 28.6, 50.9,
121, 223, and 392 mg/L was 7.88, 7.98, 8.05, 8.07, and 8.06, respectively.
115
-------
Once the test was complete, the LC50 was determined using the average measured test
concentrations with the Spearman method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 83; test results are provided in Table 84. Analytical chemistry data are provided
in Table 85. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 25.
116
-------
Table 83. Test conditions for 96-hour toxicity test on Pimephales promelas with
boron (pH 7.75).
Summary of Toxicity Test Conditions
l.
Test Species and Age:
Pimephales promelas, (weight 0.12g and 22.0 mm length),
GLEC Culture
2.
Test Type and Duration:
Continuous flow-through, 96 hours
3.
Test Dates:
October 23-October 27, 2009
4.
Test Temperature (°C):
25+1
5.
Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6.
Photoperiod:
16 h light, 8 h darkness
7.
Feeding Regime:
None
8.
Size of Test Vessel:
2.5 Liter glass Tank
9.
Volume of Test Solutions:
2000 mL
10.
No. of Test Organisms per Test Vessel:
10
11.
No. of Test Vessels per Treatment:
2
12.
Total No. of Test Organisms per Treatment:
20
13.
Target or Nominal Test Concentrations (mg/L):
400, 200, 100, 50.0, and 25.0
14.
Analytical Test Concentrations (average of samples
collected at test initiation and termination-mg/L):
392, 223, 121, 50.9, and 28.6
15.
16.
17.
Renewal of Test Solutions:
Dilution and Primary Control Water:
Test Material:
Continuous flow through, 4 turnovers per day
De-chlorinated Lake Michigan Water
Boric Acid: Sigma Aldrich, ACS Reagent >= 99.5% Cas.
No. 10043-35-3, Batch 118K0007 and Borax (sodium
tetraborate decahydrate) Sigma Aldrich, > 99.5%, ACS
reagent, Cas. No. 1303-96-4, Lot# 118K0172
18.
Secondary Control Water:
None
19.
Aeration:
None
20.
Endpoints Measured:
Mortality (LC50)
117
-------
Table 84. Test results for 96-hour toxicity test on Pimephales promelas with boron at
pH 7.75.
Results of a Pimephales promelas 96-Hour Static Acute Toxicity Test
Conducted 10/23/09 - 10/27/09 Using: Boron (Boric Acid: Sisma Aldrich Cas No. 10043-35-3)
(Borax: Sisma Aldrich Cas No. 1303-96-4)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50* Values (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
0
(0)
0
(0)
>392 289 202 137
96-Hour LCS0 *= 137 mg/L
25.0 (28.6) mg/L
0
(0)
0
(0)
0
(0)
0
(0)
50.0 (50.9) mg/L
0
(0)
0
(0)
0
(0)
0
(5)
LC50 *95% Confidence Limits
100 (121) mg/L
0
(0)
0
(0)
0
(0)
21
(21)
24-Hr
48-Hr
72-Hr
96-Hr
200 (223) mg/L
5
(5)
10
(10)
65
(65)
100
(100)
LL NA 260 178 118
UL NA 320 229 158
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
400 (392) mg/L
10
(10)
95
(95)
100
(100)
100
(100)
Method(s) Used to Determine LC50 Confidence
Limit Values: Spearman
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
118
-------
Table 85. Analytical chemistry data for 96-hour toxicity test on Pimephales promelas
with boron at pH 7.75.
Nominal (and Measured) Test
Conent rations
Boron
(mg/L)
T emperature
(°C)
PH
(S.U.)
DO
(mg/L)
SC
(mmos)
Hardness
(mg/L)
Alkalinity
(mg/L)
Ammonia
(mg/L)
Dilution
Water/Control
Average
Replicate #
1
0.05
(0.04-0.05)
24.8
(24.5-25.3)
7.63
(7.49-7.75)
7.3
(6.5-8.0)
351
146
41
ND
Replicate #
4
0.06
(0.05-0.07)
25.1
(24.9-25.4)
7.72
(7.56-7.85)
7.3
(6.4-8.1)
321
0.05
24.9
7.68
7.3
336
146
41
ND
25 n^/L
(28.4 mg/L)
Average
Replicate #
1
28.7
(27.2-31.2)
24.9
(24.5-25.4)
7.86
(7.70-7.95)
7.2
(5.9-8.1)
375
138
49
ND
Replicate #
3
28.6
(27.1-32.1)
25.0
(24.7-25.4)
7.91
(7.80-8.00)
7.4
(6.7-8.1)
345
28.6
24.9
7.88
7.3
360
138
49
ND
50 n^/L
(50.9 mg/L)
Average
Replicate #
1
51.0
(47.4-54.7)
24.7
(24.5-25.1)
7.97
(7.80-8.07)
7.2
(6.4-8.0)
393
146
57
ND
Replicate #
3
50.9
(47.5-55.5)
24.9
(24.7-25.4)
7.98
(7.90-8.07)
7.4
(6.7-8.1)
361
50.9
24.8
7.98
7.3
377
146
57
ND
100 n^/L
(121 mg/L)
Replicate #
1
121
(111-127)
24.9
(24.6-25.3)
8.06
(7.97-8.13)
7.4
(6.7-8.1)
436
128
77
ND
Replicate #
3
121
(110-130)
25.0
(24.7-25.4)
8.05
(7.96-8.13)
7.1
(6.2-8.1)
410
Average
121
24.9
8.05
7.3
423
128
77
ND
200 n^/L
(223 mg/L)
Replicate #
2
222
(216-228)
24.6
(24.4-25.1)
8.07
(7.99-8.15)
7.3
(6.6-8.0)
500
132
107
ND
Replicate #
4
225
(222-228)
25.2
(24.8-25.6)
8.07
(8.00-8.14)
7.3
(6.7-8.1)
476
Average
223
24.9
8.07
7.3
488
132
107
ND
400 mg/L
(392 mg/L)
Replicate #
1
392
(381-399)
24.7
(24.4-25.1)
8.07
(8.00-8.13)
7.3
(6.7-8.0)
616
128
168
ND
Replicate #
4
392
(384-401)
24.7
(24.4-25.2)
8.06
(8.01-8.13)
7.3
(6.4-8.0)
590
Average
392
24.7
8.06
7.3
603
128
168
ND
DO: Dissolved Oxygen ND: Non Detect; below detection limit.
SC: Specific Conductance NM: Not Measured
119
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96-hr Toxicity of Boron (yH 8.75) on Pimeyhales yromelas
The 96-hr test to determine the toxicity of boron (pH 8.75) on P. promelas was
completed by GLEC. The P. promelas (collected from the GLEC laboratory culture)
were continuously exposed for 96-hours to five concentrations of boron and to a dilution
water control with a target test pH of 8.75 (range of 8.65 to 8.85) using a continuous
flow-through system (modified Benoit mini-dilutor) and an in-line pH
adjustment/metering unit. The stock solutions used for the test were prepared by diluting
a known weight (grams) of borax and boric acid to a known volume of dilution water (de-
chlorinated Lake Michigan water). The pH of both the dilution water and stock solution
were adjusted by using sodium hydroxide. The pH adjusted stock solution was delivered
to mixing cells and diluted with pH adjusted Lake Michigan water to achieve target
nominal concentrations of boron and a target test pH of 8.75. The temperature-controlled
test concentration solutions were supplied to each test chamber via the continuous flow-
through system at a rate of approximately four turnovers a day. There were two replicate
test chambers for each treatment. The flow through test was conducted at 25 ± 1 °C with
a photoperiod of 16 hr light and 8 hr darkness (ambient laboratory light).
After test concentrations had achieved steady state in the flow through system, the test
was initiated with the P. promelas. Although these organisms were cultured in, and
acclimated to, the dilution water's "natural" pH (typically between a pH of 7.9 and 8.2),
they were not acclimated to the pH-adjusted dilution water prior to test initiation for two
reasons: 1) GLEC does not maintain a laboratory culture of P. promelas in de-
chlorinated Lake Michigan water maintained at a pH of 8.75 (and this was outside the
scope of work for this Work Assignment) and 2) adequate acclimation of the organisms
to the pH-adjusted dilution water may have jeopardized the weight requirement (0.1-5 g
at test initiation) for test organisms required under the toxicity testing method (ASTM
2007).
The P. promelas were randomly assigned to the 2.5 L glass test chambers until each test
chamber contained ten P. promelas. P. promelas were counted on a daily basis and the
number of live P. promelas was recorded. pH was recorded twice a day, at a minimum of
eight hours apart (i.e., morning and evening). The number of surviving P. promelas was
recorded at test termination (96-hours).
The test was completed at the following nominal boron concentrations: 25.0, 50.0, 100,
200, and 400 mg/L (dilution factor of 0.5). The average pH for the dilution water control
measured in the P. promelas toxicity test for the 96-hour test period was 8.75. The
average pH over the 96-hour test duration for the five test concentrations of 21.1, 42.4,
112, 219, and 376 mg/L was 8.72, 8.70, 8.70, 8.70, and 8.67, respectively.
Instantaneous water temperature measurements made on February 13, 14, 15 and 16
(Days 1-4: 23.6°C-24.7°C) exceeded the allowable range of 25 ± 1 °C outlined in the
toxicity testing method. However, the average water temperatures across the duration of
the test in each replicate were always ± 0.9 °C of the target test temperature (25 °C) in all
treatments. In addition, the water temperature as recorded by the continuous temperature
120
-------
logger did show that the test temperature fell outside the temperature allowance during
the times of 0430 to 0830 on those days. However, due to the relatively small number of
temperature readings measured outside the range, these water temperature exceedances
likely had little effect on the results of this study.
Once the test was complete, the LC50 was determined using the average measured test
concentrations with the Probit method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 86; test results are provided in Table 87. Analytical chemistry data are provided
in Table 88. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 26.
121
-------
Table 86. Test conditions for 96-hour toxicity test on Pimephales promelas with
boron (pH 8.75).
Summary of Toxicity Test Conditions
l.
Test Species and Age:
Pimephales promelas, (weight 0.13g and 22.8 mm length),
GLEC Culture
2.
Test Type and Duration:
Continuous flow-through, 96 hours
3.
Test Dates:
February 12-February 16,2010
4.
Test Temperature (°C):
25+1
5.
Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6.
Photoperiod:
16 h light, 8 h darkness
7.
Feeding Regime:
None
8.
Size of Test Vessel:
2.5 Liter glass Tank
9.
Volume of Test Solutions:
2000 mL
10.
No. of Test Organisms per Test Vessel:
10
11.
No. of Test Vessels per Treatment:
2
12.
Total No. of Test Organisms per Treatment:
20
13.
Target or Nominal Test Concentrations (mg/L):
400, 200, 100, 50.0, and 25.0
14.
Analytical Test Concentrations (average of samples
collected at test initiation and termination-mg/L):
376,219, 112, 42.4, and 21.1
15.
16.
17.
Renewal of Test Solutions:
Dilution and Primary Control Water:
Test Material:
Continuous flow through, 4 turnovers per day
De-chlorinated Lake Michigan Water
Boric Acid: Sigma Aldrich, ACS Reagent >= 99.5% Cas.
No. 10043-35-3, Batch 118K0007 and Borax (sodium
tetraborate decahydrate) Sigma Aldrich, > 99.5%, ACS
reagent, Cas. No. 1303-96-4, Lot# 118K0172
18.
Secondary Control Water:
None
19.
Aeration:
None
20.
Endpoints Measured:
Mortality (LC50)
122
-------
Table 87. Test results for 96-hour toxicity test on Pimephales promelas with boron at
pH 8.75.
Results of a Pimephales promelas 96-Hour Static Acute Toxicity Test
Conducted 02/12/10 - 02/16/10 Using: Boron (Boric Acid: Sisma Aldrich Cas No. 10043-35-3)
(Borax: Sisma Aldrich Cas No. 1303-96-4)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50* Values (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
0
(0)
5
(5)
>373 325 195 133
96-Hour LCS0 *= 133 mg/L
25.0 (21.1) mg/L
0
(0)
0
(0)
0
(0)
0
(0)
50.0 (42.4) mg/L
0
(0)
0
(0)
0
(0)
0
(0)
LC50 *95% Confidence Limits
100 (112) mg/L
0
(0)
0
(0)
5
(5)
30
(30)
24-Hr
48-Hr
72-Hr
96-Hr
200 (219) mg/L
5
(5)
15
(15)
60
(60)
95
(95)
LL NA 276 165 110
UL NA 412 227 157
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
400 (376) mg/L
10
(10)
65
(65)
100
(100)
100
(100)
Method(s) Used to Determine LC50 Confidence
Limit Values: Probit
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
123
-------
Table 88. Analytical chemistry data for 96-hour toxicity test on Pimephales promelas
with boron at pH 8.75.
Nominal (and Measured) Test
Conent rations
Boron
(mg/L)
T emperature
(°C)
PH
(S.U.)
DO
(mg/L)
SC
(mmos)
Hardness
(mg/L)
Alkalinity
(mg/L)
Ammonia
(mg/L)
Dilution
Water/Control
Average
Replicate #
1
0.04
(0.03-0.04)
24.2
(23.8-24.6)
8.74
(8.68-8.83)
7.6
(7.4-7.7)
290
126
111
ND
Replicate #
2
0.03
(0.03-0.03)
24.4
(23.8-25.2)
8.75
(8.71-8.81)
7.4
(7.1-7.6)
299
0.03
24.3
8.75
7.5
295
126
111
ND
25 n^/L
(21.1 mg/L)
Average
Replicate #
1
21.9
(21.7-22.2)
24.3
(23.8-24.8)
8.72
(8.67-8.77)
7.5
(7.4-7.7)
331
110
133
ND
Replicate #
3
20.2
(18.0-21.7)
24.2
(23.8-25.2)
8.71
(8.63-8.77)
7.3
(7.1-7.4)
336
21.1
24.2
8.72
7.4
333
110
133
ND
50 n^/L
(42.4 mg/L)
Average
Replicate #
2
42.6
(37.0-46.0)
24.3
(23.8-25.2)
8.69
(8.62-8.72)
7.1
(6.8-7.6)
368
116
156
ND
Replicate #
4
42.7
(36.3-45.4)
24.3
(23.8-24.8)
8.72
(8.67-8.77)
7.2
(7.1-7.4)
370
42.4
24.3
8.70
7.2
369
116
156
ND
100 n^/L
(112 mg/L)
Replicate #
1
111
(93.5-125)
24.3
(23.9-25.2)
8.71
(8.65-8.76)
7.3
(7.1-7.5)
472
116
235
ND
Replicate #
4
113
(96.3-128)
24.3
(23.6-25.0)
8.70
(8.63-8.76)
7.4
(7.2-7.6)
464
Average
112
24.3
8.70
7.3
471
116
235
ND
200 n^/L
(219 mg/L)
Replicate #
1
207
(185-254)
24.3
(23.6-24.8)
8.70
(8.64-8.73)
7.3
(7.0-7.6)
628
120
340
ND
Replicate #
4
231
(202-264)
24.5
(23.9-25.2)
8.70
(8.64-8.74)
7.2
(7.1-7.4)
638
Average
219
24.4
8.70
7.3
633
120
340
ND
400 mg/L
(376 mg/L)
Replicate #
1
382
(359-412)
24.1
(24.0-24.5)
8.67
(8.62-8.73)
7.2
(6.9-7.3)
844
108
510
ND
Replicate #
4
370
(353-399)
24.1
(23.8-24.8)
8.67
(8.62-8.71)
7.2
(7.1-7.3)
845
Average
376
24.1
8.67
7.2
845
108
510
ND
DO: Dissolved Oxygen ND: Non Detect; below detection limit.
SC: Specific Conductance NM: Not Measured
124
-------
Manganese
Table 89 provides a summary of estimated LC50 values for the two toxicity tests
performed using manganese. LC50 values ranged between 31.5 and 43.3 mg Mn/L.
Table 89. LC50 estimates for toxicity tests performed using manganese.
Test Species and Duration
LC50 (mg Mn/L)
Lampsilis siliquoidea 96 hr
43.3
Megalonaias nervosa - 96 hr
31.5
For each of the acute toxicity tests completed using manganese, two tables were
generated: the first summarizes the test results for each toxicity test, including nominal
and analytical test concentration and LC50 estimates with confidence intervals; the second
table summarizes analytical chemistry data collected throughout the toxicity tests. Also
discussed, if applicable, are deviations from the guidance provided in the ASTM method
used to complete the toxicity testing.
125
-------
96-hr Toxicity of Mansanese on Lamysilis siliguoidea
The 96-hr test to determine the toxicity of manganese on L. siliquoidea was completed by
INHS. Test organisms, < 5-day old juveniles collected from the Missouri State University
laboratory culture, were acclimated to the dilution water (MHRW), test temperature and
other test conditions prior to test initiation. Once acclimated, test organisms were
examined for any disease, stress, parasites, etc. If free from ailments, test organisms were
randomly assigned to the test chambers (which were randomly assigned to testing
locations); four replicates were used per treatment with five organisms per replicate. One
replicate was mistakenly loaded with only 4 individuals, but this was accounted for in the
LC50 calculation.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 150, 75, 37, 18.8, 9.4, and 4.7
mg Mn/L.
Testing was conducted at 20 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 90; test results are provided in Table 91. Analytical chemistry data are provided
in Table 92. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 27.
126
-------
Table 90. Test conditions for 96-hour toxicity test on Lampsilis siliquoidea with
manganese.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Lampsilis siliquoidea, juveniles <5 days old, Missouri State
Univbersity
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
September 8 - 12, 2009
4. Test Temperature (°C):
20+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
50 mL beaker
9. Volume of Test Solutions:
40 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg Mn/L):
150, 75,37, 18.8, 9.4, and 4.7
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination- Mn
mg/L):
154.9,72.5,34.5, 18.5, 10.1 and 4.5
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Manganese sulfate monohydrate: Fisher Scientific, ACS
grade assay, 98.7%, Cas. No. 7785-87-7, Lot # 086316 and
manganese chloride tetrahydrate, Fisher Scientific, certified
ACS Assay 99.8% Cas. No. 7773-01-5, Lot # 081484
18. Secondary Control Water:
19. Aeration:
20. Endpoints Measured:
None
None
Mortality (LC50)
127
-------
Table 91. Test results for 96-hour toxicity test on Lampsilis siliquoidea with
manganese.
Results of a Lampsilis siliauoidea) 96-Hour Static Acute Toxicity Test
Conducted 09/08/09 - 09/12/09 Usins: Manganese sulfate Cas. No. 7785-87-7 &maneanese
chloride Cas. No. 7773-01-5
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
0
0
0
62.8 50.0 48.2 43.3
96-Hour LCS0* = 43.3 mg/L
4.7 (4.5) mg/L
0
0
0
0
9.4 (10.1) mg/L
0
0
0
0
LC50 9 5% Confidence Limits
18.8 (18.5) mg/L
0
0
0
0
24-Hr
48-Hr
72-Hr
96-Hr
37 (34.5) mg/L
0
0
5.2
21.0
LL 52.7 NR 45.0 38.1
UL 74.9 NR 51.7 49.2
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
75 (72.5) mg/L
70
100
100
100
150 (154.9) mg/L
95
100
100
100
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
128
-------
Table 92. Analytical chemistry data for 96-hour toxicity test on Lampsilis siliquoidea
with manganese.
Nominal (Measured) Test
Concentration
Manganese"
Temperature
PH
D.O.
Conductivity
Alkalinity
Hardness
(mg/L)
(°C)
(S.U.)
(mg/L)
(mmos)
(mg/L)
(mg/L)
Day 0
<0.01
20.4
7.9
7.08
316
62
92
Day 1
20.3
Day 2
20.4
Day 3
19.6
Day 4
<0.01
19.1
7.9
7.50
322
62
90
Dilution water/Control
na
Day 0
4.5
20.5
7.9
7.83
324
62
98
Day 1
20.2
Day 2
20.3
Day 3
19.5
Day 4
4.6
19.1
7.9
7.69
328
62
100
4.7 (4.5) mg/L
4.5
Day 0
9.2
20.4
7.9
7.87
341
62
110
Day 1
20.3
Day 2
20.4
Day 3
19.6
Day 4
11.0
19.0
7.9
7.65
345
62
110
9.4 (10.1) mg/L
10.1
Day 0
19.0
20.4
7.7
7.83
376
62
120
Day 1
20.2
Day 2
20.3
Day 3
19.6
Day 4
18.0
19.0
7.9
7.72
380
62
124
18.8 (18.5) mg/L
18.5
Day 0
34.0
20.5
7.7
7.94
447
62
152
Day 1
20.2
Day 2
20.3
Day 3
19.6
Day 4
35.0
19.0
7.9
7.76
450
62
152
37 (34.5) mg/L
34.5
Day 0
73.0
20.5
7.7
7.88
582
62
220
Day 1
20.2
Day 2
20.3
Day 3
19.7
Day 4
72.0
19.1
7.9
7.70
590
62
224
75 (72.5) mg/L
72.5
Day 0
150.0
20.5
7.7
7.88
840
62
*
Day 1
20.3
Day 2
20.4
Day 3
19.7
Day 4
160.0
19.2
7.9
7.80
850
62
*
150 (154.9) mg/L
154.9
3Manganese Analysis Method 200.7
* interference in hardness measurement
na = not applicable
129
-------
96-hr Toxicity of Mansanese on Meealonaias nervosa
The 96-hr test to determine the toxicity of manganese onM nervosa was completed by
INHS. Test organisms, < 5-day old juveniles collected from the Genoa National Fish
Hatchery, were acclimated to the dilution water (MHRW), test temperature and other test
conditions prior to test initiation. Once acclimated, test organisms were examined for any
disease, stress, parasites, etc. If free from ailments, test organisms were randomly
assigned to the test chambers (which were randomly assigned to testing locations); four
replicates were used per treatment with five organisms per replicate. In one replicate of
the control a test organism was inadvertently crushed, but this was accounted for in the
LC50 calculation.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 300, 150, 75, 37.5, and 18.8
mg Mn/L.
Testing was conducted at 20 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 93; test results are provided in Table 94. Analytical chemistry data are provided
in Table 95. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 28.
130
-------
Table 93. Test conditions for 96-hour toxicity test on Megalonaias nervosa with
manganese.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Megalonaias nervosa, juveniles <5 days old (Genoa
National Fish Hatchery)
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
October 23 - 27,2009
4. Test Temperature (°C):
20+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
50 mL beaker
9. Volume of Test Solutions:
40 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg Mn/L):
300, 150, 75, 37.5, and 18.8
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination-mg
Mn/L):
290, 140,72, 34, and 18
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Manganese sulfate monohydrate: Fisher Scientific, ACS
grade assay, 98.7%, Cas. No. 7785-87-7, Lot # 086316 and
manganese chloride tetrahydrate, Fisher Scientific, certified
ACS Assay 99.8% Cas. No. 7773-01-5, Lot # 081484
18. Secondary Control Water:
19. Aeration:
20. Endpoints Measured:
None
None
Mortality (LC50)
131
-------
Table 94. Test results for 96-hour toxicity test on Megalonaias nervosa with
manganese.
Results of a Mesalonaias nervosa 96-Hour Static Acute Toxicity Test
Conducted 10/23/09 - 10/27/09 Usins: Manganese sulfate Cas. No. 7785-87-7 & manganese
chloride Cas. No. 7773-01-5
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
0
0
0
41.6 37.5 31.5 31.5
96-Hour LCS0* = 31.5 mg/L
18.8 (18)mg/L
0
0
0
0
37.5 (34) mg/L
30
40
65
65
LC50 9 5% Confidence Limits
75 (72) mg/L
95
100
100
100
24-Hr
48-Hr
72-Hr
96-Hr
150 (140) mg/L
100
100
100
100
LL 35.6 32.2 27.2 27.2
UL 48.8 43.6 36.6 36.6
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
300 (290) mg/L
100
100
100
100
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
132
-------
Table 95. Analytical chemistry data for 96-hour toxicity test on Megalonaias nervosa
with manganese.
Nominal (Measured) Test Concentration
Manganese"
Temp.
PH
D.O.
Cond.
Alkalinity
Hardness
Ammonia
(mg/L)
(°C)
(S.U.)
(mg/L)
(mmos)
(mg/L)
(mg/L)
(mg/L)
Day 0
<0.01
20.8
8.0
8.34
303
60
90
<0.05
Day 1
20.9
8.1
8.04
297
Day 2
20.9
8.1
8.13
303
Day 3
20.9
8.0
8.31
305
Day 4 total
0.1
20.8
8.0
8.00
315
60
92
<0.05
Dilution
Day 4 dissolved
0.7
water/Control
0.1
Day 0
18.0
20.8
7.9
8.33
375
60
112
<0.05
Day 1
20.9
7.9
8.10
362
Day 2
20.9
7.7
8.19
371
Day 3
20.9
7.6
8.31
379
Day 4 total
18.0
20.8
7.8
7.92
383
60
112
<0.05
Day 4 dissolved
16.0
18.8 (18.0) mg/L
18.0
Day 0
33.0
20.8
7.9
8.26
445
60
*
<0.05
Day 1
20.9
7.7
8.11
442
Day 2
20.9
7.6
8.25
458
Day 3
20.9
7.6
8.42
478
Day 4 total
35.0
20.8
7.7
7.94
495
60
*
<0.05
Day 4 dissolved
34.0
37.5 (34.0) mg/L
34.0
Day 0
70.0
20.8
7.9
8.29
582
60
*
<0.05
Day 1
20.9
7.7
8.07
569
Day 2
20.8
7.6
8.26
567
Day 3
20.9
7.5
8.32
581
Day 4 total
74.0
20.9
7.7
7.91
589
62
*
<0.05
Day 4 dissolved
68.0
75 (72.0) mg/L
72.0
Day 0
140.0
20.8
7.8
8.33
841
60
*
<0.05
Day 1
20.9
7.7
8.03
826
Day 2
20.8
7.6
8.22
817
Day 3
20.9
7.5
8.40
851
Day 4 total
140.0
20.9
7.6
7.88
848
62
*
<0.05
Day 4 dissolved
150.0
150(140.0) mg/L
140.0
Day 0
290.0
20.8
7.7
8.27
1333
60
*
<0.05
Day 1
20.9
7.7
7.98
1301
Day 2
20.8
7.6
8.20
1300
Day 3
20.9
7.5
8.22
1325
Day 4 total
290.0
20.9
7.6
7.94
1340
62
*
<0.05
Day 4 dissolved
290.0
300 (290) mg/L
290.0
aManganese Analysis Method 200.7
interference in hardness measurement
Temp. = temperature; Cond. = conductivity
133
-------
Fluoride
Table 96 provides a summary of estimated LC50 values for the two toxicity tests
performed using fluoride. LC50 values ranged between 13.4 and 62.0 mg F/L.
Table 96. LC50 estimates for toxicity tests performed using fluoride.
Test Species and Duration
LCjo (mg F/L)
Sphaerium simile - 96 hr
62.0
Hyalella azteca - 96 hr
13.4
For each of the acute toxicity tests completed using fluoride, two tables were generated:
the first summarizes the test results for each toxicity test, including nominal and
analytical test concentration and LC50 estimates with confidence intervals; the second
table summarizes analytical chemistry data collected throughout the toxicity tests.
134
-------
96-hr Toxicity of Fluoride on Sphaerium simile
The 96-hr test to determine the toxicity of fluoride on S. simile was completed by INHS.
Test organisms, juveniles released from field collected adults, were acclimated to the
dilution water (MHRW), test temperature and other test conditions prior to test initiation.
Once acclimated, test organisms were examined for any disease, stress, parasites, etc. If
free from ailments, test organisms were randomly assigned to the test chambers (which
were randomly assigned to testing locations); four replicates were used per treatment with
five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 800, 400, 200, 100, and 50
mg F/L.
Testing was conducted at 22 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 97; test results are provided in Table 98. Analytical chemistry data are provided
in Table 99. Accompanying information, including raw laboratory data, analytical
chemistry data and statistical analyses, is provided in Appendix 29.
135
-------
Table 97. Test conditions for 96-hour toxicity test on Sphaerium simile with fluoride.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Sphaerium simile, juveniles (released from field-collected
adults)
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
July 13 - 17, 2009
4. Test Temperature (°C):
22+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
150 mL beaker
9. Volume of Test Solutions:
120 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg F/L):
800, 400, 200, 100, and 50
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination-mg
F/L):
800,390, 185, 88 and 44
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Sodium fluoride: Acros Organics, 99+% for analysis ACS,
Cas. No. 7681-49-5, Lot # A0243428
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
136
-------
Table 98. Test results for 96-hour toxicity test on Sphaerium simile with fluoride.
Results of a Svhaerium simile 96-Hour Static Acute Toxicity Test
Conducted 07/13/09 - 07/17/09 Using: Sodium fluoride: Sisma Aldrich Cas. No. 7681-49-5
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
0
0
5
>800 >800 >800 62
96-Hour LCsn* = 62 mg/L
50 (44) mg/L
0
0
0
25
100 (88) mg/L
0
0
0
75
LC50 9 5% Confidence Limits
200 (185) mg/L
0
0
35
100
24-Hr
48-Hr
72-Hr
96-Hr
400 (390) mg/L
0
5
50
100
LLNR NR NR 51
UL NR NR NR 75
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
800 (800) mg/L
0
5
25
100
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
• All LC50 and EC50 values are determined based on measured concentrations.
137
-------
Table 99. Analytical chemistry data for 96-hour toxicity test Sphaerium simile with
fluoride.
Nominal (Measured) Test
Concentration
Fluoride"
Temperature
PH
D.O.
Conductivity
Alkalinity
Hardness
(mg/L)
(°C)
(S.U.)
(mg/L)
(nnihos)
(mg/L)
(mg/L)
Day 0
<0.5
22.9
8.0
7.76
307
62
96
Day 1
22.9
7.97
Day 2
22.8
8.00
Day 3
22.7
7.63
Day 4
<0.5
22.9
8.0
8.05
310
62
96
Dilution water/Control
na
Day 0
45
22.8
8.1
7.82
555
64
94
Day 1
22.8
7.92
Day 2
22.8
7.83
Day 3
22.7
7.70
Day 4
44
22.9
8.0
8.04
558
68
80
50 (44) mg/L
44
Day 0
86
22.8
8.1
7.80
785
66
80
Day 1
22.9
8.11
Day 2
22.8
7.95
Day 3
22.7
7.67
Day 4
91
22.9
8.1
7.53
784
68
62
100 (88) mg/L
88
Day 0
190
22.9
8.0
7.82
1264
78
76
Day 1
22.8
8.05
Day 2
22.9
7.82
Day 3
22.8
6.63
Day 4
180
22.9
8.1
6.03
1268
82
50
200 (185) mg/L
185
Day 0
400
22.9
8.2
7.78
2210
120
64
Day 1
22.9
7.99
Day 2
22.8
7.79
Day 3
22.8
4.88
Day 4
380
23.0
7.9
5.49
2210
120
30
400 (390) mg/L
390
Day 0
800
23.0
8.2
7.82
4050
160
14
Day 1
22.9
8.05
Day 2
22.9
8.02
Day 3
22.8
6.51
Day 4
800
22.9
8.0
6.89
4090
160
2
800 (800) mg/L
800
3 Fluoride Analysis Method 300.0
na = not applicable
138
-------
96-hr Toxicity of Fluoride on Hyalella azteca
The 96-hr test to determine the toxicity of fluoride on H. azteca was completed by
GLEC. H. azteca were collected from GLEC's laboratory culture. These organisms are
maintained in 10 gallon glass aquaria; plastic artificial turf and screen mesh serve as a
substrate for the culture. The tanks are filled with de-chlorinated Lake Michigan water
(City of Traverse City, Michigan water passed through an activated carbon filter).
Cultures are fed 50 mL of 4 g/L Tetrafin slurry daily. When visible algae are not
observed within the glass aquaria, algae {Selenastrum sp.) are used as a supplement to the
Tetrafin slurry. Additionally, on occasion, dried Aspen (Populus sp.) leaves are prepared
as a food supplement. The culture is maintained in a 16-hour light: 8-hour dark
photoperiod at a temperature between 23 and 26 °C.
Test organisms were acclimated to the dilution water (MHRW), test temperature and
other test conditions prior to test initiation. Once acclimated, test organisms were
examined for any disease, stress, parasites, etc. If free from ailments, test organisms were
randomly assigned to the test chambers (which were randomly assigned to testing
locations); four replicates were used per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 7.8, 12.9, 21.6, 36.0, 60.0,
and 100 mg F/L.
Testing was conducted at 22 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Probit and Spearman Karber methods.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 100; test results are provided in Table 101. Analytical chemistry data are
provided in Table 102. Accompanying information, including raw laboratory data,
analytical chemistry data, reference toxicant data and statistical analyses, is provided in
Appendix 30.
139
-------
Table 100. Test conditions for 96-hour toxicity test on Hyalella azteca with fluoride.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Hyalella azteca, 14 days old, GLEC culture
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
September 17- September 21,2009
4. Test Temperature (°C):
22+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
150 mL beaker
9. Volume of Test Solutions:
100 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Target or Nominal Test Concentrations (mg F/L):
100, 60, 36,21.6, 12.9, and 7.8
14. Analytical Test Concentrations (average of samples
collected at test initiation and termination-mg F/L):
89.4, 58.9, 32.7,22.8, 14.3, and 8.5
15. Renewal of Test Solutions:
16. Dilution and Primary Control Water:
17. Test Material:
18. Secondary Control Water:
19. Aeration:
20. Endpoints Measured:
None
USEPA MHRW
Sodium Fluoride: Sigma Aldrich, 99+% ACS Reagent Cas.
No. 7681-49-4, Batch # 06810JJ
None
None
Mortality (LC50)
140
-------
Table 101. Test results for 96-hour toxicity test on Hyalella azteca with fluoride.
Results of a Hvalella arleca 96-Hour Static Acute Toxicity Test
Conducted 09/17/09 - 09/21/09 Using: Fluoride (Sodium Fluoride Sisma Aldrich Cas. No. 7681-49-4)
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
(0)
0
(0)
0
(0)
0
(0)
25.1 16.0 13.8 13.4
96-Hour LCS0* = 13.4 mg/L
7.8 (8.5) mg/L
0
(0)
0
(0)
0
(0)
0
(0)
12.9 (14.3) mg/L
0
(0)
45
(45)
70
(70)
70
(70)
LC50 95% Confidence Limits
21.6 (22.8) mg/L
20
(20)
80
(80)
90
(90)
95
(95)
24-Hr
48-Hr
72-Hr
96-Hr
36 (32.7) mg/L
100
(100)
100
(100)
100
(100)
100
(100)
LL 23.3 13.9 12.0 11.8
UL 27.1 18.3 15.6 15.2
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
60 (58.9) mg/L
100
(100)
100
(100)
100
(100)
100
(100)
100 (89.4) mg/L
100
(100)
100
(100)
100
(100)
100
(100)
Method(s) Used to Determine LC50 Confidence
Limit Values: Probit and Spearman-Karber
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 values are determined based on measured concentrations.
141
-------
Table 102. Analytical chemistry data for 96-hour toxicity test on Hyalella azteca
with fluoride.
Nominal (and M
Test Concent
easured)
ations
Fluoride3
(mg/L)
Temperature
(°C)
pH
(s.u.)
D.O.
(mg/L)
Conductivity
(mmhos)
Alkalinity
(mg/L)
Hardness
(mg/L)
Ammonia
(mg/L)
Dilution
water/Control
Day 0
ND
22.0
7.84
8.0
300
54
80
ND
Day 1
21.9
7.79
7.5
Day 2
22.1
7.79
7.1
Day 3
22.0
7.84
7.9
Day 4
ND
22.2
7.81
8.0
325
68
88
ND
ND
7.8 (8.5) mg/L
Day 0
8.4
22.0
7.84
8.0
368
Day 1
22.0
7.78
7.5
Day 2
22.1
7.83
7.3
Day 3
22.0
7.91
7.7
Day 4
8.6
22.2
7.87
8.0
363
8.5
12.9(14.3) mg/L
Day 0
14.1
22.0
7.85
8.0
393
Day 1
22.0
7.85
7.5
Day 2
22.1
7.88
7.4
Day 3
21.9
7.97
8.1
Day 4
14.5
22.2
7.89
8.0
377
14.3
21.6(22.8) mg/L
Day 0
22.0
22.0
7.85
8.0
432
Day 1
21.9
7.90
7.5
Day 2
22.0
7.89
7.3
Day 3
21.8
8.02
8.0
Day 4
23.5
21.9
7.97
8.0
422
66
100
22.8
36 (32.7) mg/L
Day 0
33.4
22.0
7.88
8.0
502
Day 1
32.0
21.9
7.91
7.5
Day 2
Day 3
Day 4
32.7
60(58.9) mg/L
Day 0
58.1
22.0
7.88
8.0
622
Day 1
59.6
21.9
7.92
7.5
Day 2
Day 3
Day 4
58.9
100(89.4) mg/L
Day 0
91.0
22.0
7.89
8.0
781
72
68
ND
Day 1
87.7
21.9
7.99
7.5
70
68
Day 2
Day 3
Day 4
89.4
a Fluoride Analysis EPA 300.0
ND Not Detect; below detection limit
142
-------
Chloride
Table 103 provides a summary of the estimated LC50 value for the toxicity test performed
using chloride.
Table 103. LC50 estimate for toxicity test performed using chloride.
Test Species and Duration
LC50 (mg Cl/L)
Musculium transversum - 96 hr
1,930
For the acute toxicity test completed using chloride, two tables were generated: the first
summarizes the test results for the toxicity test, including nominal and analytical test
concentration and LC50 estimates with confidence intervals; the second table summarizes
analytical chemistry data collected throughout the toxicity test.
143
-------
96-hr Toxicity of Chloride on Musculium transversum
The 96-hr test to determine the toxicity of chloride on M. transversum was completed by
INHS. Test organisms, juveniles released from field collected adults, were acclimated to
the dilution water (MHRW), test temperature and other test conditions prior to test
initiation. Once acclimated, test organisms were examined for any disease, stress,
parasites, etc. If free from ailments, test organisms were randomly assigned to the test
chambers (which were randomly assigned to testing locations); four replicates were used
per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 8,000, 4,018, 2,028, 1,032,
and 535 mg Cl/L.
Testing was conducted at 22 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 104; test results are provided in Table 105. Analytical chemistry data are
provided in Table 106. Accompanying information, including raw laboratory data,
analytical chemistry data and statistical analyses, is provided in Appendix 31.
144
-------
Table 104. Test conditions for 96-hour toxicity test on Musculium transversum with
chloride.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Musculium transversum, juveniles (released from field-
collected adults)
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
September 04 - 08, 2009
4. Test Temperature (°C):
22+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
150 mL beaker
9. Volume of Test Solutions:
120 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg Cl/L):
8000, 4018, 2028, 1032, and 535
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination-
mg/L):
7750, 3899, 1900, 985 and 505
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
EPA MHRW
17. Test Material:
Sodium chloride: Sigma Aldrich, 99% pure ACS Reagent
Cas. No. 7647-14-5, Lot # B0110043
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
145
-------
Table 105. Test results for 96-hour toxicity test on Musculium transversum with
chloride.
Results of a Musculium transversum 96-Hour Static Acute Toxicity Test
Conducted 09/04/09 - 09/08/09 Usins: Sodium chloride: Sisma Aldrich Cas. No. 7647-14-5
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
5
5
5
2722 2455 2455 1930
96-Hour LCsn* = 1930 mg/L
535 (505) mg/L
0
0
0
0
1032 (985) mg/L
0
0
0
0
LC50 9 5% Confidence Limits
2028(1900) mg/L
0
15
15
50
24-Hr
48-Hr
72-Hr
96-Hr
4018(3899) mg/L
100
100
100
100
LLNR 2200 2200 1655
ULNR 2740 2740 2251
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
8000 (7750) mg/L
100
100
100
100
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
146
-------
Table 106. Analytical chemistry data for 96-hour toxicity test on Musculium
transversum with chloride.
Nominal (Measured) Test
Concentration
Chloride"
Sulfate"
Temperature
PH
D.O.
Conductivity
Alkalinity
Hardness
(mg/L)
(mg/L)
(°C)
(S.U.)
(mg/L)
(linihos)
(mg/L)
(mg/L)
Day 0
33
58
23.0
8.1
7.93
385
62
48
Day 1
22.9
Day 2
23.0
Day 3
23.0
Day 4
39
59
23.0
8.0
8.13
378
62
48
Dilution water/Control
36
Day 0
490
58
22.9
8.1
7.99
2010
62
48
Day 1
23.0
Day 2
22.9
Day 3
22.9
Day 4
520
59
23.0
8.0
8.14
2000
62
48
535 (505) mg/L
505
Day 0
970
59
22.8
8.1
8.00
3620
62
48
Day 1
22.9
Day 2
22.8
Day 3
22.9
Day 4
1000
59
23.0
8.0
8.22
3640
62
48
1032 (985) mg/L
985
Day 0
1900
58
22.9
8.0
8.05
6700
62
48
Day 1
23.0
Day 2
22.9
Day 3
23.0
Day 4
1900
60
23.0
8.0
8.14
6730
62
48
2028 (1900) mg/L
1900
Day 0
3800
58
22.9
8.0
8.05
12520
62
48
Day 1
23.0
Day 2
22.7
Day 3
22.9
Day 4
4000
59
22.9
8.1
8.06
12470
62
48
4018 (3899) mg/L
3899
Day 0
7700
60
22.8
8.0
8.07
24200
62
48
Day 1
22.9
Day 2
22.9
Day 3
22.8
Day 4
7800
60
22.9
7.9
8.14
24000
62
48
8000 (7750) mg/L
7750
" Chloride Analysis Method 300.0
b Sulfate Analysis Method 300.0
147
-------
Sulfate
Table 107 provides a summary of estimated LC50 values for the two toxicity tests
performed using sulfate. LC50 values ranged between 1,483 and 3,378 mg S04/L.
Table 107. LC50 estimates for toxicity tests performed using sulfate.
Test Species and Duration
LC50 (mg S04/L)
Ligumia recta - 96 hr
1,483
Megalonaias nervosa - 96 hr
3,378
For each of the acute toxicity tests completed using sulfate, two tables were generated:
the first summarizes the test results for each toxicity test, including nominal and
analytical test concentration and LC50 estimates with confidence intervals; the second
table summarizes analytical chemistry data collected throughout the toxicity tests.
148
-------
96-hr Toxicity of Sulfate on Lisumia recta
The 96-hr test to determine the toxicity of sulfate on L. recta was completed by INHS.
Test organisms, < 5-day old juveniles collected from the Missouri State University
laboratory culture, were acclimated to the dilution water (MHRW), test temperature and
other test conditions prior to test initiation. Once acclimated, test organisms were
examined for any disease, stress, parasites, etc. If free from ailments, test organisms were
randomly assigned to the test chambers (which were randomly assigned to testing
locations); four replicates were used per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 5,000, 2,545, 1,317, 703, and
397 mg S04/L.
Testing was conducted at 20 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 108; test results are provided in Table 109. Analytical chemistry data are
provided in Table 110. Accompanying information, including raw laboratory data,
analytical chemistry data and statistical analyses, is provided in Appendix 32.
149
-------
Table 108. Test conditions for 96-hour toxicity test on Ligumia recta with sulfate.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Ligumia recta, juveniles <5 days old, Missouri State
University
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
September 10 - 14, 2009
4. Test Temperature (°C):
20+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
50 mL beaker
9. Volume of Test Solutions:
40 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg SO4/L):
5,000,2,545, 1,317, 703, and 397
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination-mg
SO4/L):
5,038,2,349, 1,200, 665, and 370
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
USEPA MHRW
17. Test Material:
Sodium sulfate anhydrous: Sigma Aldrich, 99% pure Cas.
No. 7757-82-6, Lot # A0231747
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
150
-------
Table 109. Test results for 96-hour toxicity test on Ligumia recta with sulfate.
Results of a Lisumia recta 96-Hour Static Acute Toxicity Test
Conducted 09/10/09 - 09/14/09 Using: Sodium sulfate: Sisma Aldrich Cas. No. 7757-82-6
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
0
0
5
3440 3440 1938 1483
96-Hour LCsn* = 1483 mg/L
397 (370) mg/L
0
0
0
0
703 (665) mg/L
0
0
0
5
LC50 9 5% Confidence Limits
1317(1200) mg/L
0
0
0
15
24-Hr
48-Hr
72-Hr
96-Hr
2545 (2349) mg/L
0
0
80
100
LLNR NR 1705 1321
ULNR NR 2203 1665
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
5000 (5038) mg/L
100
100
100
100
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
a Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
* All LC50 and EC50 values are determined based on measured concentrations.
151
-------
Table 110. Analytical chemistry data for 96-hour toxicity test on Ligumia recta with
sulfate.
Nominal (Measured) Test
Concentration
Sulfate"
Chlorideb
Temperature
PH
D.O.
Conductivity
Alkalinity
Hardness
(mg/L)
(mg/L)
(°C)
(S.U.)
(mg/L)
(mmos)
(mg/L)
(mg/L)
Day 0
83
3.0
19.8
7.9
7.93
309
60
92
Day 1
19.7
Day 2
19.8
Day 3
19.2
Day 4
85
2.5
19.2
8.0
7.85
308
62
92
Dilution water/Control
84
Day 0
380
2.6
19.8
8.0
7.95
1029
60
92
Day 1
19.7
Day 2
19.8
Day 3
19.2
Day 4
360
2.7
19.2
8.2
7.87
1032
62
92
397 (370) mg/L
370
Day 0
660
2.8
19.8
8.1
7.95
1691
60
92
Day 1
19.8
Day 2
19.9
Day 3
19.2
Day 4
670
2.9
19.2
8.3
7.92
1716
60
92
703 (665) mg/L
665
Day 0
1200
3.9
19.8
8.0
7.94
2840
60
92
Day 1
19.9
Day 2
19.9
Day 3
19.2
Day 4
1200
3.3
19.2
8.3
7.96
2990
60
92
1317 (1200) mg/L
1200
Day 0
2300
4.3
19.8
8.1
7.99
5280
60
92
Day 1
19.9
Day 2
20.0
Day 3
19.2
Day 4
2400
4.8
19.2
8.4
7.95
5380
60
92
2545 (2349) mg/L
2349
Day 0
4700
5.9
19.9
8.1
8.08
9570
62
94
Day 1
19.9
Day 2
20.0
Day 3
19.2
Day 4
5400
6.1
19.2
8.4
8.00
9680
64
94
5000 (5038) mg/L
5038
" Sulfate Analysis Method 300.0
b Chloride Analysis Method 300.0
152
-------
96-hr Toxicity of Sulfate on Meealonaias nervosa
The 96-hr test to determine the toxicity of sulfate on M nervosa was completed by INHS.
Test organisms, < 5-day old juveniles collected from the Genoa National Fish Hatchery,
were acclimated to the dilution water (MHRW), test temperature and other test conditions
prior to test initiation. Once acclimated, test organisms were examined for any disease,
stress, parasites, etc. If free from ailments, test organisms were randomly assigned to the
test chambers (which were randomly assigned to testing locations); four replicates were
used per treatment with five organisms per replicate.
Organisms were exposed to a dilution water control and the test chemical at varying
concentrations under static conditions. Serial dilutions of the highest test concentration
(known weight of test chemical dissolved in a known volume of dilution water) were
made to prepare the following nominal test concentrations: 5,000, 2,545, 1,317, 703, and
397 mg S04/L.
Testing was conducted at 20 ± 1 °C with a photoperiod of 16 hr light and 8 hr dark
(ambient laboratory light). Organisms were not fed for the duration of the test and were
examined daily for mortality. Once the test was complete, the LC50 value was determined
using the Spearman-Karber method.
A summary of the toxicity test conditions present throughout the assessment are provided
in Table 111; test results are provided in Table 112. Analytical chemistry data are
provided in Table 113. Accompanying information, including raw laboratory data,
analytical chemistry data and statistical analyses, is provided in Appendix 33.
153
-------
Table 111. Test conditions for 96-hour toxicity test on Ligumia recta with sulfate.
Summary of Toxicity Test Conditions
1. Test Species and Age:
Megalonaias nervosa, juveniles <5 days old, Genoa
National Fish Hatchery
2. Test Type and Duration:
Static, 96 hours
3. Test Dates:
October 16 - 20,2009
4. Test Temperature (°C):
20+1
5. Light Quality:
Ambient Laboratory, 10-20 |xE/m2/s
6. Photoperiod:
16 h light, 8 h darkness
7. Feeding Regime:
None
8. Size of Test Vessel:
50 mL beaker
9. Volume of Test Solutions:
40 mL
10. No. of Test Organisms per Test Vessel:
5
11. No. of Test Vessels per Treatment:
4
12. Total No. of Test Organisms per Treatment:
20
13. Test Concentrations (mg S04/L):
5,000,2,545, 1,317, 703, and 397
14. Analytical Test Concentrations (geometric mean of
samples collected at test initiation and termination- mg
S04/L):
5,130,2,498, 1,249, 690, and 380
15. Renewal of Test Solutions:
None
16. Dilution and Primary Control Water:
EPA MHRW
17. Test Material:
Sodium sulfate anhydrous: Sigma Aldrich, 99% pure Cas.
No. 7757-82-6, Lot # A0231747
18. Secondary Control Water:
None
19. Aeration:
None
20. Endpoints Measured:
Mortality (LC50)
154
-------
Table 112. Test results for 96-hour toxicity test on Megalonaias nervosa with sulfate.
Results of a Meealonaias nervosa 96-Hour Static Acute Toxicity Test
Conducted 10/16/09 - 10/20/09 Using: Sodium sulfate: Sisma Aldrich Cas. No. 7757-82-6
Nominal (Measured)
Concentrations
Cumulative Percent Affected a
LC50 Values* (mg/L)
24-Hr
48-Hr
72-Hr
96-Hr
24-Hr
48-Hr
72-Hr
96-Hr
Primary Control/
Dilution Water
0
0
0
0
3,564 3,564 3,378 3,378
96-Hour LCsn* = 3,378 mg/L
397 (380) mg/L
5
5
10
10
703 (690) mg/L
0
0
5
5
LC50 9 5% Confidence Limits
1317(1249) mg/L
0
0
0
0
24-Hr
48-Hr
72-Hr
96-Hr
2545 (2498) mg/L
0
0
10
10
LL NR NR 3,021 3,021
ULNR NR 3,777 3,777
LL = Lower Limit
UL = Upper Limit
NR = Confidence Intervals are not reliable
5000 (5130) mg/L
100
100
100
100
Method(s) Used to Determine LC50 and EC50
Confidence Limit Values: Spearman-Karber
Cumulative percent affected is the total percentage of test organisms observed dead, immobile, exhibiting
loss of equilibrium or other defined endpoints.
• All LC50 and EC50 values are determined based on measured concentrations.
155
-------
Table 113. Analytical chemistry data for 96-hour toxicity test on Megalonaias
nervosa with sulfate.
Nominal (Measured) Test
Concentration
Sulfate"
Chloride"
Temperature
PH
D.O.
Conductivity
Alkalinity
Hardness
(mg/L)
(mg/L)
(°C)
(S.U.)
(mg/L)
(mmos)
(mg/L)
(mg/L)
Day 0
83
2.4
21.0
7.8
7.95
300
60
88
Day 1
20.9
7.8
8.30
300
Day 2
20.5
7.8
8.21
308
Day 3
20.9
7.9
8.28
324
Dilution
Day 4
90
2.4
20.6
7.9
8.18
364
68
94
water/Control
86
2.4
Day 0
380
2.4
21.0
8.0
8.24
1021
60
88
Day 1
20.8
7.9
8.38
1016
Day 2
20.4
7.9
8.22
1020
Day 3
20.9
8.0
8.40
1021
Day 4
380
2.6
20.7
8.0
8.20
1104
68
88
397 (380) mg/L
380
2.5
Day 0
680
2.7
21.0
8.0
8.21
1702
62
90
Day 1
20.9
8.0
8.35
1695
Day 2
20.4
8.0
8.23
1698
Day 3
20.9
8.0
8.29
1696
Day 4
700
2.8
20.7
8.0
8.25
1853
68
88
703 (690) mg/L
690
2.7
Day 0
1200
3.1
21.0
8.1
8.28
2940
62
90
Day 1
21.0
8.0
8.32
2920
Day 2
20.5
8.0
8.28
2930
Day 3
20.9
8.0
8.40
2940
Day 4
1300
3.3
20.8
8.0
8.24
3230
68
90
1317 (1249) mg/L
1249
3.2
Day 0
2400
4.0
21.0
8.2
8.22
5320
62
90
Day 1
20.9
8.1
8.30
5280
Day 2
20.5
8.1
8.20
5295
Day 3
20.8
8.2
8.38
5290
Day 4
2600
4.3
20.8
8.1
8.20
5600
68
92
2545 (2498) mg/L
2498
4.1
Day 0
4700
5.7
21.0
8.3
8.26
9550
62
94
Day 1
21.0
8.2
8.28
9540
Day 2
20.5
8.2
8.00
9540
Day 3
20.8
8.3
8.32
9540
Day 4
5600
6.3
20.9
8.2
8.37
10320
68
92
5000 (5130) mg/L
5130
6.0
" Sulfate Analysis Method 300.0
b Chloride Analysis Method 300.0
156
-------
REFERENCES
American Public Health Association (APHA), American Water Works Association
(AWW A) and Water Environment Federation (WEF). 2006 a. Standard Methods for the
Examination of Water and Wastewater. Method 2340 C (Hardness).
American Public Health Association (APHA), American Water Works Association
(AWW A) and Water Environment Federation (WEF). 2006 b. Standard Methods for the
Examination of Water and Wastewater. Method 2320 B (Alkalinity).
American Public Health Association (APHA), American Water Works Association
(AWW A) and Water Environment Federation (WEF). 2006 c. Standard Methods for the
Examination of Water and Wastewater. Method 4500-NH3 (D).
American Public Health Association (APHA), American Water Works Association
(AWW A) and Water Environment Federation (WEF). 2006 d. Standard Methods for the
Examination of Water and Wastewater. Method 4500 NO3 Nitrogen (Nitrate).
American Public Health Association (APHA), American Water Works Association
(AWW A) and Water Environment Federation (WEF). 2006 e. Standard Methods for the
Examination of Water and Wastewater. Method 4500 NO2 Nitrogen (Nitrite).
American Society for Testing and Materials (ASTM). 2005. Standard Guide for
Conducting Early Life-Cycle Toxicity Tests with Fishes. Standard E1241-05 in Vol.
11.06 of the Annual Book of ASTM Standards. ASTM International, West
Conshohocken, PA.
American Society for Testing and Materials (ASTM). 2006 a. Standard Guide for
Conducting Laboratory Toxicity Tests with Freshwater Mussels. Standard E2455-06 in
Vol. 11.06 of the Annual Book of ASTM Standards. ASTM International, West
Conshohocken, PA.
American Society for Testing and Materials (ASTM). 2006 b. Standard Guide for
Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia. Standard
E1295-01 in Vol. 11.06 of the Annual Book of ASTM Standards. ASTM International,
West Conshohocken, PA.
American Society for Testing and Materials (ASTM). 2007. Standard Guide for
Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and
Amphibians. Standard E729-96 in Vol. 11.06 of the Annual Book of ASTM Standards.
ASTM International, West Conshohocken, PA.
BenoitD.A., V.R. Mattson, and D.I. Olson. 1982. A continuous-flow mini-diluter system
for toxicity testing. Water Res. 16:457-464.
157
-------
REFERENCES (CONT'D)
Great Lakes Environmental Center (GLEC). 2008. Standard Operating Procedure for the
Determination of Nitrate Plus Nitrite Nitrogen in Surface Water Samples Using
Automated Hydrazine Reduction. SOP Number: CHM 2008. Effective Date: September
25, 1997. Revision Date: May 5, 2008. 13 pgs.
Great Lakes Environmental Center (GLEC). 2009 a. Standard Operating Procedure for
Acute Static Toxicity Tests for Test Materials with Ceriodaphnia dubia. SOP Number:
TOX 0047. Effective Date: June 2, 2009. Revision Date: June 16, 2009. 6 pgs.
Great Lakes Environmental Center (GLEC). 2009 b. Standard Operating Procedure for
Acute Static Toxicity Tests for Test Materials with Chironomus dilutus. SOP Number:
TOX 0045. Effective Date: May 7, 2009. Revision Date: June 16, 2009. 7 pgs.
Great Lakes Environmental Center (GLEC). 2009 c. Standard Operating Procedure for
Acute Static Toxicity Tests for Test Materials with Fathead Minnows. SOP Number:
0044. Effective Date: May 7, 2009. Revision Date: June 19, 2009. 7 pgs.
Great Lakes Environmental Center (GLEC). 2009 d. Standard Operating Procedure for
Acute Static Toxicity Tests for Test Materials with Hyalella azteca. SOP Number: 0046.
Effective Date: May 7, 2009. Revision Date: June 16, 2009. 7 pgs.
Great Lakes Environmental Center (GLEC). 2009 e. Standard Operating Procedure for
Early Life-Stage Flow-Through Toxicity Tests with Pimephalespromelas (Fathead
Minnow). SOP Number: TOX 0043. Effective Date: June 2, 2009. Revision Date: June
16, 2009. 10 pgs.
Great Lakes Environmental Center (GLEC). 2009 f. Standard Operating Procedure for
Operation of the Continuous Flow-Through Exposure System. SOP Number: TOX 0030.
Effective Date: September 1, 1996. Revision Date: June 16, 2009. 4 pgs.
Great Lakes Environmental Center (GLEC). 2009 g. Standard Operating Procedure for
Static-Renewal Short-Term Chronic Toxicity Tests for Test Materials with Ceriodaphnia.
SOP Number: TOX 0042. Effective Date: June 2, 2009. Revision Date: June 16, 2009. 8
Pgs.
Great Lakes Environmental Center (GLEC). 2009 h. Standard Operating Procedure for
the Colorimetric Determination of Nitrite Nitrogen in Water Samples Using the Milton
Roy Spectronic 1200 Spectrophotometer. SOP Number: CHM 2015. Effective Date:
April 12, 1999. Revision Date: May 14, 2009. 9 pgs.
Great Lakes Environmental Center (GLEC). 2009 i. Quality Assurance Project Plan:
Nitrate, Nitrite, Boron, Manganese, Fluoride and Chloride Toxicity Testing. Prepared for
U.S. EPA, Office of Science and Technology, Health and Ecological Criteria Division.
EPA Contract #EP-C-09-001, Work Assignment B-12. 33 pgs.
158
-------
REFERENCES (CONT'D)
Gulley, D.D. 1996. TOXSTAT 3.5. Western Eco Systems Technology, Incorporated.
Cheyenne, WY, October 1996.
Illinois Natural History Survey (INHS). 2009. Standard Operating Procedure for Acute
Static Toxicity Tests for Test Material with Sphaerium simile, Musculium sp., stonefly
nymphs, and Lampsilis siliquoidea. Effective Date: June 8, 2009. 5 pgs.
Simmerman, R.V. 2008. Standard Operating Procedure for the Analysis of Metals using
ICP-MS Technique. Completed for SOS Analytical on October 1, 2008. 8 pgs.
Underwriters Laboratories (UL). 2008 a. Standard Operating Procedure for General
Chemistry: Inorganic Anions in Water by Ion Chromatography. SOP Number: UL-SBN-
GenChem-008-17. Effective Date: June 17, 2008. 24 pgs.
Underwriters Laboratories (UL). 2008 b. Standard Operating Procedure for Metals:
Determination of Trace Elements in Water by Inductively Coupled Plasma-Atomic
Emission Spectrometry (ICP-AES). SOP Number: UL-SBN-MET-007-09. Effective
Date: November 11, 2008. 29 pgs.
Underwriters Laboratories (UL). 2009 a. Standard Operating Procedure for General
Chemistry: Fluoride in Water by Ion Selective Electrode. SOP Number: UL-SBN-
GenChem-010-11. Effective Date: March 4, 2009. 17 pgs.
Underwriters Laboratories (UL). 2009 b. Standard Operating Procedure for Metals:
Determination of Trace Elements in Water by Inductively Coupled Plasma-Mass
Spectrometry (ICP-MS). SOP Number: UL-SBN-MET-004-17. Effective Date: February
19, 2009. 28 pgs.
United States Environmental Protection Agency (EPA). 1971. Method 354.1:
Determination of Nitrite by Spectrophotometry.
United States Environmental Protection Agency (EPA). 1978. Method 353.1:
Determination of Nitrate-Nitrite by Colorimetry.
United States Environmental Protection Agency (EPA). 1985. Guidelines for Deriving
Numerical National Water Quality Criteria for the Protection of Aquatic Organisms and
Their Uses. PB85-227049. Washington, DC 20460.
United States Environmental Protection Agency (EPA). 1993. Method 300.0:
Determination of Inorganic Anions by Ion Chromatography.
United States Environmental Protection Agency (EPA). 1994. Method 200.7:
Determination of Metals and Trace Elements in Water and Wastes by Inductively
Coupled Plasma - Atomic Emission Spectrometry.
159
-------
REFERENCES (CONT'D)
United States Environmental Protection Agency (EPA). 1994. Method 200.8:
Determination of Trace Elements in Water and Wastes by Inductively Coupled Plasma -
Mass Spectrometry.
United States Environmental Protection Agency (EPA). 2002 a. Methods for Measuring
the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine
Organisms, Fifth Addition. Office of Water, Washington, DC. EPA-821-R-02-012.
United States Environmental Protection Agency (EPA). 2002 b. Short-term Methods for
Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater
Organisms, Fourth Addition. Office of Water, Washington, DC. EPA-821-R-02-013.
160
-------
Appendix 1: Raw Data for 96-hr Toxicity of
Nitrate on Lampsilis siliquoidea
-------
Raw Laboratory Data
-------
96 Hour Acute Toxicity Test Data S
Water: gfA. fe S-ZU-ol
heet
Toxicant: t0>»-h raj~e
siart Date/Time: Woj" 3o» O.
Initials i A>>
0 hrs
24 hrs
48 hrsj
Is*"
¦f
96 hrs!
c
31
_4-
*C"
Initials
% survival
0
temp.
pH
D.O.
Alk.
... 3o-
i
Uo
24hra
\W
48 firs
7o.»
72 hrs
?Q, I
\q
R o
3 to
¦1 ''I
(,1.
Ohr*
L
24 hrs I
48 hrs I
_SL_
5
72 hrs!
96 hrs
—Si
_2L
,Q
^ I 2-*
S . o\
t>o
¦V 82,
X
0 hrej *s '
rep;{
24 hrs!
48 hrs
72 hrs
96 hrs|
trealraent:!
y
-i-L
-S~
96 hrs
"to. \
eencf
Ik!"
y o
ft-o.
y O
1,12-
.yz.
^T"
24 hrs
48 hrs
Cp"
72 hrs
96 hrsf
treartonent: I
p ni^
i-
IT
0 hrsi
24 hrsj
48 hrs
72 hrsj
96 hrsj
J2—
o
a
_a_
X-
0 hrs
% survive
temp
48 hns
pi;
-if °
¦^rw
\i.%
12 hrs
96 hrs
"Z,0 • €>
io.o
Z<
D.O
Alk
53152!
<7To
bo
°l €>
initials
0 hrs
% survival
temp
!L& t
24 hrs
9L
48 hrs
O
pf
COfrf
tTt
I"), ")
72 hrs
06 firs
~to. 6
*S. ^
IT.?
Si mo
Alk
- \
%,\%
Wo
f Q
¦a 7-
-------
Analytical Chemistry Data
-------
Dave Soucek. INHS
Nitrate in Water
Analyst: Duane Kimme
ABE Water Quality Lab
File Name: SDav©5.N03
L. siliquoidea
y x
Std Cone
Pk Ht
4.00
141.5
2.00
75.5
LOO
38.5
0.50
19
0,25
11.5
SUMMARY OUTPUT
Regression Statistics
Multiple R 0 9099133
R Square 0.«9982fi5
Adjusted R Square 0.9^96531
Standard Error 0.0142256
Observations 3
ANOVA
d! 55—
—m
h Significance h
Regression
1 1.1664643
1.1664643
5764.083333 0.008384745
Residual
1 0.0002024
0.0002024
Total
2 1.1666667
Coeftbients
Slmd&d Error
tstat
P-vate
Lower 95%
Jcper 95.0%
intercept
-0.01310330&
0.017576408
-0.745509746
0.592167911
-0.23S431473
"ill u "*¦ i
0.210224856
X Vadable 1
0.0266113.53
0,000350-511
75.9215604
0.008384745
0.022157666
1 t-r ! i ^->36
0.031065019
EPA 383.1
Sample ID
Date
dilution
peak height
N03+N02 as H mg L
expected
difference
A N03
Ofr'03/09
1
0.0
-0.01
0
0
BN03
Q&0m9
100
28.0
73
70
3
CN03
06/03/09
too
54.5
144
141
3
DN03
06'03'09
1Q0
103.0
283
282
1
E N03
06'0a'09
200
105.5
582
564
18
F N03
06/03/09
1000
44.0
1158
1129
29
A N03
06/07/09
1
0.0
-0.01
0
-0.01
BN03
06-07/09
100
28.5
75
70
5
CN03
06/07/09
100
54,0
142
141
1
DN03
06/07/09
100
108.0
298
282
16
CONTROL
1
38,5
1,01
1
0.01
E N03
06/07/09
200
105.0
579
564
15
F N03
06/07/09
1000
43.0
1131
1129
2
C N03 rep
06/07/09
100
56.0
148
141
7
C N03 spk
06/07/09
100
84.0
226
227
-1
DW
1
1,0
0.01
0.00
0.01
DW spk
1.0
37.0
0.97
1.00
-0.03
EXQC
1.0
38.0
1.00
1.00
0.00
CONTROL
1.0
38.0
1.00
1.00
0.00
-------
SUMMARY OUTPUT
gression Statistics
Multiple R 1
R Square 1
Adjusted R Square 65535
Standard Error 0
Observations 2
ANOVA
d[ SS MS F Significance F
Regression 1 2 2 #NUM! #NUM!
Residua} 0 0 65535
Total 1 2
Coefficients Ss&nJsrd Error t Stat P-vafas Lowsr 95% Upper 95% Lc*wer95.Q% tipper 95.0%
intercept -0.267978/88 0 65535 #NUMI -0.287678788 -0.287S78788 -0.28787B7W -0,287878788
X Variable 1 0.03030303 0 65535 #NUM! 0.03030303 0.03030303 0.03030303 0,CC<030303
-------
Statistical Analyses
-------
CT-TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS
SPEARMAN-KARBER
TRIM: 15.00%
LC50: 814.567
95% LOWER CONFIDENCE: 730.031
95% UPPER CONFIDENCE: 908.893
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
74.00 20. 0. .00 .9537D-04
143.00 20. 0. .00 .9537D-04
291.00 20. 1. 5.00 .2003D-02
580.00 20. 3. 15.00 .1288D+00
1144.00 20. 17. 85.00 .1288D+00
THE BINOMIAL TEST SHOWS THAT 580.00 AND 1144.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.7423 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 814.567
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
2 .120 814.57 660.13 1103.28
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
7 .186 1.00 .37
SLOPE = 5.05
95% CONFIDENCE LIMITS: 2.87 AND 7.23
LC50= 781.70
95% CONFIDENCE LIMITS: 642.71 AND 979.43
LCI = 270.83
95% CONFIDENCE LIMITS: 122.41 AND 380.24
DATE: June 3, 2009 TEST NUMBER: DURATION: 24 h
SAMPLE: N-N03 SPECIES: Lampsilis siliquoidea
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 814.567 580.000 ******* 564.000
MAA 814.567 660.135 ******* 443.146
PROBIT 781.703 642.713 979.429 336.717
SPEARMAN 814.567 730.031 908.893 178.862
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: .00%
LC50: 597.690
95% LOWER CONFIDENCE: 498.470
95% UPPER CONFIDENCE: 716.660
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
74.00 20. 0. .00 .9537D-04
143.00 20. 1. 5.00 .2003D-02
291.00 20. 2. 10.00 .2012D-01
580.00 20. 6. 30.00 .5766D+01
1144.00 20. 20. 100.00 .9537D-04
THE BINOMIAL TEST SHOWS THAT 291.00 AND 1144.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9798 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 675.613
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
3 .066 563.09 460.54 720.22
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
7 1.344 3.74 .01
SINCE THE PROBABILITY IS LESS THAN 0.05,
RESULTS CALCULATED USING THE PROBIT METHOD
PROBABLY SHOULD NOT BE USED.
SLOPE = 4.04
95% CONFIDENCE LIMITS: -.64 AND 8.73
LC50= 580.52
95% CONFIDENCE LIMITS: 0 AND + INFINITY
LCI = 154.20
95% CONFIDENCE LIMITS: 0 AND 353.67
DATE: June 3, 2009 TEST NUMBER: DURATION: 48 h
SAMPLE: N-N03 SPECIES: Lampsilis siliquoidea
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 675.613 291.000 ******* 853.000
MAA 563.086 460.542 720.216 259.675
PROBIT 580 521 ******* ******* *******
SPEARMAN 597.690 498.470 716.660 218.190
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 10.00%
LC50: 491.325
95% LOWER CONFIDENCE: 382.615
95% UPPER CONFIDENCE: 630.922
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
74.00 20. 3. 15.00 .1288D+00
143.00 20. 1. 5.00 .2003D-02
291.00 20. 4. 20.00 .5909D+00
580.00 20. 11. 55.00 .4119D+02
1144.00 20. 20. 100.00 .9537D-04
THE BINOMIAL TEST SHOWS THAT 291.00 AND 1144.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.4090 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 528.264
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
3 .066 453.33 371.07 561.96
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
6 1.573 5.17 .00
SINCE THE PROBABILITY IS LESS THAN 0.05,
RESULTS CALCULATED USING THE PROBIT METHOD
PROBABLY SHOULD NOT BE USED.
SLOPE = 2.44
95% CONFIDENCE LIMITS: -.62 AND 5.50
LC50= 417.59
95% CONFIDENCE LIMITS: 0 AND + INFINITY
LCI = 46.46
95% CONFIDENCE LIMITS: 0 AND 179.87
DATE: June 3, 2009 TEST NUMBER: DURATION: 72 h
SAMPLE: N-N03 SPECIES: Lampsilis siliquoidea
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 528.264 291.000 ******* 853.000
MAA 453.330 371.074 561.956 190.883
PROBIT 417 587 ******* ******* *******
SPEARMAN 491.325 382.615 630.922 248.307
NOTE: MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING.
ADJUSTMENTS WERE MADE PRIOR TO SPEARMAN-KARBER ESTIMATION.
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 25.00%
LC50: 357.151
95% LOWER CONFIDENCE: 250.443
95% UPPER CONFIDENCE: 509.323
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
74.00 20. 5. 25.00 .2069D+01
143.00 20. 5. 25.00 .2069D+01
291.00 20. 7. 35.00 .1316D+02
580.00 20. 15. 75.00 .2069D+01
1144.00 20. 20. 100.00 .9537D-04
THE BINOMIAL TEST SHOWS THAT 143.00 AND 580.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 95.8611 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 375.216
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
4 .110 275.18 198.87 376.05
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
6 .129 1.00 .05
SLOPE = 2.04
95% CONFIDENCE LIMITS: 1.31 AND 2.77
LC50= 259.74
95% CONFIDENCE LIMITS: 183.65 AND 361.18
LCI = 18.77
95% CONFIDENCE LIMITS: 3.98 AND 40.09
DATE: June 3, 2009 TEST NUMBER: DURATION: 96 h
SAMPLE: N-N03 SPECIES: Lampsilis siliquoidea
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 375.216 143.000 580.000 437.000
MAA 275.185 198.874 376.051 177.177
PROBIT 259.739 183.648 361.183 177.535
SPEARMAN 357.151 250.443 509.323 258.879
**** = LIMIT DOES NOT EXIST
-------
Appendix 2: Raw Data for 96-hr Toxicity of
Nitrate on Megalonaias nervosa
-------
Raw Laboratory Data
-------
l'(t Hour Walci Litlumii Aeu.1. 7est Data S!"k." o trealniei.N
To\ie;tnC t
I'V'JJL
i .
(Species: (
^A.0*
t
iX H> V
, i
Rate •,»
' . 1 i I
iJ.
.. 1 tB-t C^i
Water: .. ¦ r.
•PAV
1 1 ¦ o '
< uiurc <
5AU ft xtv
1 < t,
Stall line 'i ; ' |
-Age: r,. -
.
(initials
jA"'P i |^fv^
Temp/Light'
W '
c i
't rlasswaie-
h*-''
nn t <5 vt
1 1 i <¦ 1
Stir s t\
J
| -
1
Treatment
rep
(i h
24 h
48 1:
72 h
^r. W
k ,H-ni iih
J4h
4Kb
"i,
1 ~J:h
1
,
,
-
temp
,,
V
¦
¦>
,»H
V ^ \y
i
> -
v-eml
3
" i -
¦I
c
no.
>
I
V 1^.
Alk"
a
X
1 lard
i
x [
X
i i.
I
temp
i
1
-
„
S"
pi i
-
; *
!-¦
%
---
.. i _
eoitd
. • -i
IT-.,
,
4
-
«:
, i -¦>
DO
' i
1
, 1 \ \
A Ik-
X
>
\
Hard
\
X • X
' !
1
r -
¦ '*
temp
! - I
1
*>
'
pf]
.
0 ,
, (
-
L ¦¦¦:
torid
1 1
' -1 M
4
'
f
D.O.
<
2-5'
\ s«i
v
Alk
X
K
X
i,: > _
ffard
,
X
X
I
-
'
temp
i
?
r
£™
PH
-
.
^.. 1
, !
! '
3
"
etmd
M fj f 0
<
U
L- '*
4
D.O,
* ' ^.,.
• . I
All
\
X
¥
I
*
Hard
1
X
X
A
;
I
-
1
temp
\ ¦
I
pll
4 t
t-
3
S.
; jcond
% W"11 o
, * ' 5
; •
4
5' I ,3
5
l
D.O
*\ ' k >
i -,L-
i i / i
Alk
X
•• •
\
Y
!
H-.ird
n
X
s
V
i
temp
, »
2LO °l
T
pll
^ 1
1 -
t
J
,
,l
cond
^ )
f Si «^
;
4
[
-
D.O
% ^ 3 $ ^
^ .
\
Mfc
X
X
\
V 1
:lard
'»
X
X
X
l
Initials ]
' * '
{ ,
-------
Analytical Chemistry Data
-------
Dave Soucek, INHS
Nitrate/Nitrite in Water
Analysis Date:10 30 09
Analyst: Duana Kimme
ABE Water Quality Lab
M. nervosa in nitrate
observed expected % diff
Sample ID Date Time dilution N03+N02 as N mg L N02-N mg/L N03-N mg L
A
10/23/09 In
1
0
0
-------
Statistical Analyses
-------
CT-TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS
SPEARMAN-KARBER
TRIM: 10.00%
LC50: 1202.642
95% LOWER CONFIDENCE: 990.638
95% UPPER CONFIDENCE: 1460.015
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
138.00
20.
0.
.00
.9537D-04
279.00
19.
0.
.00
.1907D-03
533.00
20.
0.
.00
.9537D-04
1108.00
20.
8.
40.00
.2517D+02
2002.00
20.
18.
90.00
.2012D-01
THE BINOMIAL TEST SHOWS THAT 533.00 AND 2002.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9798 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 1233.070
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
2 .076 1217.03 1038.94 1456.21
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
7 .194 1.00 .96
SLOPE = 6.58
95% CONFIDENCE LIMITS: 3.69 AND 9.48
LC50= 1243.80
95% CONFIDENCE LIMITS: 1039.15 AND 1467.73
LCI = 551.32
95% CONFIDENCE LIMITS: 275.47 AND 735.87
DATE: 10/23/2009 TEST NUMBER: DURATION: 24 h
SAMPLE: nitrate SPECIES: Megalonaias nervosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL ******* 533 000 ******* *******
MAA ******* ******* ******* 417 273
PROBIT ******* ******* ******* 428 579
SPEARMAN ******* 990.638 ******* 469.376
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: .00%
LC50: 1035.021
95% LOWER CONFIDENCE: 893.334
95% UPPER CONFIDENCE: 1199.181
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L
EXPOSED
DEAD
DEAD PROB.(%)
138.00
20.
0.
.00
.9537D-04
279.00
19.
0.
.00
.1907D-03
533.00
20.
0.
.00
.9537D-04
1108.00
20.
11.
55.00
.4119D+02
2002.00
20.
20.
100.00
.9537D-04
THE BINOMIAL TEST SHOWS THAT 533.00 AND 2002.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9998 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 1055.738
WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT
WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER
THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE
ANY STATISCALLY SOUND RESULTS.
DATE: 10/23/2009 TEST NUMBER: DURATION: 48 h
SAMPLE: nitrate SPECIES: Megalonaias nervosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL ******* 533 000 ******* *******
MA A ******* ******* ******* *******
******* ******* ******* *******
SPEARMAN ******* 893.334 ******* 305.847
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: .00%
LC50: 968.752
95% LOWER CONFIDENCE: 841.230
95% UPPER CONFIDENCE: 1115.604
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
138.00
20.
0.
.00
.9537D-04
279.00
19.
0.
.00
.1907D-03
533.00
20.
0.
.00
.9537D-04
1108.00
20.
13.
65.00
.1316D+02
2002.00
20.
20.
100.00
.9537D-04
THE BINOMIAL TEST SHOWS THAT 533.00 AND 2002.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9998 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 973.531
WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT
WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER
THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE
ANY STATISCALLY SOUND RESULTS.
DATE: 10/23/2009 TEST NUMBER: DURATION: 72 h
SAMPLE: nitrate SPECIES: Megalonaias nervosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 973.531 533.000 ******* *******
MA A H
-------
CT-TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS
SPEARMAN-KARBER
TRIM: .00%
LC50: 937.225
95% LOWER CONFIDENCE: 818.372
95% UPPER CONFIDENCE: 1073.339
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
138.00
20.
0.
.00
.9537D-04
279.00
19.
0.
.00
.1907D-03
533.00
20.
0.
.00
.9537D-04
1108.00
20.
14.
70.00
.5766D+01
2002.00
20.
20.
100.00
.9537D-04
THE BINOMIAL TEST SHOWS THAT 533.00 AND 2002.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9998 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 940.036
WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT
WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER
THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE
ANY STATISCALLY SOUND RESULTS.
DATE: 10/23/09 TEST NUMBER: DURATION: 96 h
SAMPLE: nitrate SPECIES: Megalonaias nervosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 940.036 533.000 ******* *******
MA A H
-------
Appendix 3: Raw Data for 48-hr Toxicity of
Nitrate on Chironomus dilutus
-------
Raw Laboratory Data
-------
Nt-Wa
TEST MATERIAL:
PROJECT NUMBER:^ _M2dl
TEST SPECIES:
TYPE OF TEST:
48-HOUR STATIC ACUTE TOXICITY TEST
b
0- cbljjtHiD
INCUBATOR NUMBER:
NUMBER OF ORGANISMSiCHAMBER:
NUMBER OF CHAMBERS:,
AGE OF ORGANISMS:
DILUTION WATER:,
JMJteaL-Ofii.
M M fin rAlnr.1
GLC AND/OR BATCH NUMBER:
TEST TEMPERATURE:
IQdfiuy
LIGHT INTENSITY (10-20 pe/lVr/S};.
it.o
PHOTOPERIOP: 16-.8
DATE
TEST
DAY
TECH.
INITIALS
TREATMENT LEVEL
CONTROL
^%m/L
la
s
S
4
H
H
4
H |H
M
4
4
4 3
OBSERVATIONS
j
TEMPERATURE (*C)
Qt 3
213
Pi.q
31-H
pH
190
7
7-pb
7-?^
l.m
DO (mg/L)
in
in
nn
11
n.t
<3
SP, CONDUCTANCE
!/rmhos.fcm)
s*
€
2
4
NUMBER LIVE
5
s
s
S"
5
9
€
9
M
H
4
5
3
3
3
3
i
3
a
#
0
0
OBSERVATIONS
TEMPERATURE ('C)
2a
3
^>3. .3
3
PH
iSl
1.%°)
1.W
3
DO (mgIL)
*~l • (jp
~1. S
-7-S
"7. "7
no
^0^
^ooo
21HO
Observation Key:
DOB - Dried Out on
IMM - Immobile
P - File
Beaker PM - Particulate Matter
FS - Film on Surface
F- Floater
tt\ laq K'.^o
\u>\ 3H.0
fr.lt.? (Y. xv*
W
^SD-!23°'
6QoQ^1u
^REVIEWED BY
DATE:
?S'»
-7ED
ft'- (fl-M
3. a.
By
ft', |
Cs4
-------
Analytical Chemistry Data
-------
CHAIN OF CUSTODY RECORD
{To be completed and submitted with samples)
GleC
Great Lakes Envircnn-ianfcai Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.giee.com
Great Lakes Environmental Center
Facility:
, . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page.
\ of 5
7/23/09
Mailee Gaiton/Jamie Sax ton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below) j
j
Q
O
«
i11 jpi, 5
H
tSgSfi
•.festers?
JflHsg
2
t
¦Z
Nitrite
nii'i • : •
IBIIIIIS!
CW-00-01
7/34709
23
125 mL
HDPE
Mod. Hard Control -
nitrate/nitrite
h2so4
. ¦¦
*3 -
it®
\
CW-00-02
7/34709
22
125 mL
HDPE
Mod. Hard Control -
nitrate/nitrite
Cold
in
s pi % gg
-
X
CW-00-03
7/34709
P?
125 mL
HDPE
Stock 1.5 g/L - nitrite
h2so4
111
iSB
HHf
-\
mm
CW-00-Q4
7/24709
*2
125 mL
HDPE
Stock 1.5 g/L - nitrite
Cold
Si
' V.
¦
tig
X
"iS-"-3
CW-00-G5
7/34709
3-3
125 inL
HDPE
100 mg/L - nitrite
h2so4
j"
X
CW-00-06
7/24709
5*3
125 mL
HDPE
60 mg/L - nitrite
h2so4
M sM|
X
Shipper:
1.
2.
BODi = Biochemical Orygeii Deinand-5 day "
NHj:jN = Ammonij-Nitrogci!
NO1/NO2 -N = Nitrate/Nilrite-Nitrogcn
Date/Time:
•Pcfmifiofis'
_ rV* "
TDS =Tota! Dissolve Solids
TK.N - ['otal Kjcldahl Nitrogen
Receiver:
at
Date/Time:
Temperature:
TP = Total Phosphorus
TSS - Total Suspender! .Solid1;
NOi=N ="Nitrite iffitrogeigP
NO, -N = Nitrate Nitrogen
' febtttstod analyses
* - TotaTNSf«al Nitrogen "
SRP = Soluble Reacove Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
•"2. t-T
Page " of _
Gi.kC
Qrsat Lakes Environ rnertia? Comer
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
7/23/09
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location:
Date:
Contact Person: Mailee Garton/Jamie Saxton
Email Address:
Phone Number:
Collector:
m. gaiton
Bottle ID
CW-00-13
CW-00-14
CW-00-15
CW-00-16
ew-oo-17
CW-00-18
Date / Time
of Sample
i/p4m
21
7M/09
iMim
?y
7/34709
7/24/09
*3
ir&m
*9
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HOPE
HDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
36 mg/L - nitrite
21.6 mg/L - nitrite
12.96 mg/L - nitrite
7.78 mg/L - nitrite
Stock 41.1 g/L -
nitrate
Stock 41.1 g/L -
nitrate
Check the requested analysis (see definitions below'
Preservation
Cold
Cold
Cold
Cold
H2S04
Cold
Shipper:
Date/Time:
1.
2.
LL,
vVkw C&'IQ
Receiver:
A,
Date/Time:
Temperature:
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
4
of
GleC
^rsat Lates Srwironrnanial Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person;
Email Address:
Phone Number:
Collector:
7/23/09
m. garton
Bottle ID
CW-00-19
CW-0G-20
CW-00-21
CW-00-22
CW-00-23
CW-00-24
Date /Time
of Sample
7/24/09
23
7/?4f09
1/&/09
7/2-N - Nitrite Nitrogen Total N = Total Nitrogen
NO'i-N = Nitrate Nitrogen .SRi'- Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page ^ of -
7/23/09
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax:231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
r . Traverse City, MI
Location:
Date:
Contact Person: Mailee Garton/Jamie Saxton
Email Address:
Phone Number:
Collector: m- gartOT
Bottle ID
CW-00-25
CW-00-26
CW-00-27
CW-00-28
Date / Time
of Sample
nMtm
7/24/09
PS
7M/09
7/24?09
Volume
Collected
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HOPE
HDPE
HOPE
Sample Description
(Site Information /
sample type)
1800 mg/L - nitrate
1080 mg/L - nitrate
648 mg/L - nitrate
389 mg/L - nitrate
Shipper:
1.
2.
,Au^-
Date/Time:
oQ'-^o
Receiver:
Date/Time:
7'?y-6<;
Temperature:
BODj = Biochemical Oxygen Demand-5 day TDS = Total i>issolye'Soligen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
1 „f
7/25/09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below"
a
0
aa
Chloride
Chlorophyll a
. ¦;y«. ' •
2
i
S
z;
1
TDS J
I
a,
f-
• -H:-
. •'© •.
¦+*•
98
m .
**
ft "¦
• F—l
U
Z.
CW-02-0I
fs **** //•*
aMPMi
yfvst&l
125 mL
HDPE
Mod. Hard Control -
nitrate
h2so4
X
CW-02-02
125 mL
HDPE
Mod. Hard Control -
nitrite
Cold
X
CW-02-03
-7/2S/61
125 mL
HDPE
100 mg/L — nitrite
H2SO4
K-
X
CW-02-04
Vas/d
125 mL
HDPE
60 mg/L - nitrite
h2so4
X
CW-02-05
i/zsto^
125 mL
HDPE
36 mg/L - nitrite
h2so4
X ¦ .
CW-02-06
ifcstcn
125 mL
HDPE
21.6 mg/L-nitrite
h2so4
X
Shipper:
i wnaQj; (A-
2.
Date/Time:
11 \0yo
Receiver:
Date/Time:
7-^0-
Temperature:
BODj = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS - Total Dissolve Solids
TKN - Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for 1
NO2 -N = Nitrite Nitrogen
N05 -N = Nitrate Nitrogen
Total N = Total Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
vvww.glec.com
Great Lakes Environmental Center
Facility:
r . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
of
7/25/09
Mai lee Garton/Jamie Saxton
m. garton
Check the requested analysis (see definitions below'
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Alkalinity
w
a
0
n
Chlor de
Chlorophyll a
.'-'so '
-Vi
4k
... ro- -
. -ji .
car
¦ p.
Z.
1
r»J
a
z
. -j-
.--.h
0 ¦
-¦¦-m -
"'-rj. .
: O '
:
j TDS
Bw
1-
TSS
Nitrate j
Nitrite
CW-02-15
125 mL
I1DPE
3000 mg/L — nitrate
h2so4
X
CW-02-16
i ftSteQ
125 mL
HDPE
1800 mg/L — nitrate
h2so4
;• •'r
x-:;".
CW-02-17
125 mL
HDPE
1080 mg/L — nitrate
h2so4
X'-v
CW-02-18
125 mL
HDPE
648 mg/L - nitrate
h2so4
X-V
CW-02-19
"tft&fCft
125 mL
HDPE
389 mg/L - nitrate
h2so4
XV
CW-02-20
125 mL
HDPE
3000 mg/L - nitrate
Cold
X
1.
2.
Shipper:
(AJ^djAK\flLiAAJ
Date/Time:
Receiver:
7 !03c>
Date/Time:
n-vr-di
Temperature:
BODs «= Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NO3/NO2-N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -N - Nitrite Nitrogen Total N — Total Nitrogen
NO] -N = Nitrate "Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
739 Hastings St
Traverse City, Mi 49686
Phone:231-941-2230
Fax: 231-941-2240
wvw.elec.com
Great Lakes Environmental Center
Facility:
. Traverse City. MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
of
7/25/09
Mailee Garton/Jamie Saxton
m. garton
Bottle ED
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below'
~
Alkalinity
m
0
0
m
-1
a
u
Chlorophyll a
Hardness
Z
2
O •
.¦ .JST, ¦
O
a
H
n ;•
3*
H
v®.
¦~u-
, <¦
• mm
¦ ^ '
4J
'£
z,
CW-G2-21
125 mL
HOPE
1800 mg/L - nitrate
Cold
X
CW-02-22
125 mL
HOPE
10B0 mg/L-nitrate
Cold
Sv 'i-
X
CW-02-23 "
7 ml6*
125 mL
HDPE
648 mg/L - nitrate
Cold
" • -r
v/:: -¦
X
CW-02-24 <
-Jlzeloi
125 mL
HOPE
389 mg/L - nitrate
Cold
=:
X
V
i!"=-
.
Shipper: Date/Time: Receiver: Date/Time: Temperature:
Itmlw mo aJ -7-2
2.
¦¦ - - .. .. ¦ ¦ . ¦ .. ' . I . ...
Definitions
BODs = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj-N - Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NOj/NOz -N = Nitrate/Nitrite-Nitrogen
K02 -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
GleC
PREPARATION OF RECONSTITUTED WATER
WATER TYPE:
inmOt Lakes Environ m«ntal Center
BATCH NUMBER: \101.
TOTAL VOLUME;
DATE PREPARED: "~7/7sb/07
DATA BY:
SALTS
AMOUNT ADDED TO FINAL VOLUME
NOTES
NaHCO,
z.
oo
CaSOK " 2H,0
f ^ ">o
wgso.
L
. "5o
KCI
WATER SOURCE:
RECONSTITUTED HARD WATER - WATER QUALITY
Date: J/t-lSf Checked By:
M.
SPECIFIC CONDUCTANCE
TOTAL HARDNESS
SAMPLE VOLUME
DILUTED TO
7 c>
mL TITRANT USED
2- I
mg/L CaCOa
TOTAL ALKALINITY
SAMPLE VOLUME
DILUTED TO
mL TITRANT USED
ma£L C
Cmt
CaC03
a
Hardness;
End mis:
Start mis: t).(
Alkalinity: o ^
Endmis:
Start mis
-------
PROJECT#; 6112-12 Date 23/07/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Plouride
Test Day 0
CALIBRATION
Concentrations
Eppm)
Natural log
Millivolts
0.5
-0.6931472
-39.8
1
0
-49.1
10
2.30258509
-107.7
25
3.21887582
-131.3
50
3.91202301
-149.6
Regression Output:
Constant
-52.948914
Std Err of Y Est
3.286102
R Squared
0.996630
No. of Observations
5
Degrees of Freedom
3
X Coefficients) -24.341788
Std ErrofCoef. 0,8172274
OUTPUT
Test
Organism
GLC
Number
Test
Day
Test
Material
Milli-
volts
Natural
Log
Predicted
Ammonia
Levels
Ammonia
Minus
Blank
c. difutus
Con-MH
0
water
23.9
-3.157078
0.04254991
0.00689001
c. dilutus
3000 mg/L Nitrate
0
water
21.2
-3.046157
0.04754127
0.01188137
n/a
Blank
0
n/a
28.2
-3.333729
0.0356599
0
-------
Nitrate Day 0 Chironomus diiutus Analytical Results 7,23.09
LabSamplelO
SampleDescription
ParamID
Results Units
RL LabQualffier
AnalysisMethod
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Person
HBQ7240052
3000mg/L-NHrate
N03
2690 mg/L
553.375
A4500F
7/24/2009
7/24/2009 Ben Cook
7/24/200S
Ben Cook
HB07240052
3000mg/L-Nitrate
N02
0.0082 mg/L
0.018425 J
A450QB
7/24/2009
7/24/2009 JB
7/24/2009
JB
HBO72400 53
1600mg/L-Nitrate
NQ2
0.0079 mg/L
0.01 S425 J
A45O0B
7/24/2009
7/24/2009 JB
7/24/2009
JB
HB07240053
1800mg/L-Nitrate
N03
1440 mg/L
221.35
A4500F
7/24/2006
7/24/2009 Ben Cook
7/24/2009
Ben Cook
HB07240054
1080mg/L-Nitrate
N03
1130 mg/L
110.675
A4500F
7/24/2009
7/24/2009 Ben Cook
7/24/2009
Ben Cook
HB07240054A
1080mg/L-Nitrate
NQ2
0.0053 mg/L
0.016425 J
A4500B
7/24/2009
7/24/2009 JB
7/24/2009
JB
HB07240054B
1080mg/L-Nitrale
N02
0.0043 mg/L
0.016425 J
A4500B
7/24/2009
7/24/2009 JB
7/24/2009
JB
HB07240055
648mg/L-Nrtrate
N02
<0.0036 mg/L
0.016425 U
A4500B
7/24/2009
7/24/2009 JB
7/24/2009
JB
HB07240055
64Smg/L-Nitrate
N03
832 mg/L
44.27
A4500F
7/24/2009
7/24/2009 Ben Cook
7/24/2009
Ben Cook
HB07240056
389mg/L-Nitrate
N02
<0.0036 mg/L
0.016425 U
A4500B
7/24/2009
7/24/2009 JB
7/24/2009
JB
HB07240056
389mg/L-Nitrate
NQ3
370 mg/L
22.135
A4500F
7/24/2009
7/24/2009 Ben Cook
7/24/2009
Ben Cook
HB07240057
Mod. Hand Control-nitrate/nitrite
N02
<0.0036 mg/L
0.016425 U
A4500B
7/24/2009
7/24/2009 JB
7/24/2009
JB
HB07240057A
Mod. Hard Control-nitrate/nitrite
N03
<0.0053 mg/L
0.022135 U
A4500F
7/24/2009
7/24/2009 Ben Cook
7/24/2009
Ben Cook
HB07240057B
Mod. Hard Control-nitrate/nitrite
N03
<0.0053 mg/L
0.022135 U
A4500F
7/24/2009
7/24/2009 Ben Cook
7/24/2009
Ben Cook
HB07240065
Stock 41.1g/L nitrate
N02
<0.0036 mg/L
0.016425 U
A4500B
7/24/2009
7/24/2009 JB
7/24/2009
JB
HB07240065
Stock 4l,lg/L nitrate
N03
33700 mg/L
2213.5
A4500F
7/31/2009
7/24/2009 Ben Cook
7/31/2009
Ben Cook
Page 1
-------
Nitrate Day 2 Chironomus dilutus Analytical Results 7.25.09
LabSamplelD
SampleDescription
ParamID
Results
Units
RL LabQualifier
AnalysisMethod
AnalysisDate
SampleDate
Analyst Calibration Date
Calibration Person
HBQ7250Q97
3000mg/L-nitrata
N03
2810
mg/L
221.35
A4500F
27/07/2009
25/07/2009
Ben Cot* 7/27/2009
Ben Cook
HB07250097
3000mg/L-nitrate
N02
0.0568
mg/L
0.016425
A4500B
27/07/2009
25/07/2009
JB 7/27/2009
JB
HB072S0098
1800mg/L-nitrate
N03
1855
mg/L
110.675
A4500F
27/07/2009
25/07/2009
Ben Cook 7/27/2009
Ben Cook
HB07250098
1800mg/L-nitrate
N02
0.0367
mg/L
0.016425
A4500B
27/07/2009
25/07/2009
JB 7/27/2009
JB
HB07250099
1080mg/L-nitrate
N03
1133
mg/L
44.27
A4500F
27/07/2009
25/07/2009
Ben Cook 7/27/2009
Ben Cook
HB0725Q099
1080mg/L-nitrate
N02
0,0361
mg/L
0.016425
A4500B
27/07/2009
25/07/2009
JB 7/27/2009
JB
HB072S0100
648mg/l-nitrate
N03
636
mg/L
44.27
A450QF
27/07/2009
25/07/2009
Ben Cook 7/27/2009
Ben Cook
HB07250100
648mg/L-nitrate
N02
0.0237
mg/L
0.016425
A4500B
27/07/2009
25/07/2009
JB 7/27/2009
JB
HB07250101
389mg/L-nitrate
N03
435
mg/L
22.135
A4500F
27/07/2009
25/07/2009
Ben Cook 7/27/2009
Ben Cook
HBQ7250101
389mg/L-nitrate
N02
0,0493
mg/L
0.016425
A4500B
27/07/2009
25/07/2009
JB 7/27/2009
JB
HB07250090
Mod. Hard Control-nitrite
N03
<0.0053 mg/L
0.022135 U
A4500F
27/07/2009
25/07/2009
Ben Cook 7/27/2009
Ben Cook
HB07250090
Mod. Hard Control-nitrite
N02
0.0276
mg/L
0.016425
A45O08
27/07/2009
25/07/2009
JB 7/27/2009
JB
Page 1
-------
Reference Toxicant Data
-------
GleC Chironomus dilutus Survival
NaCl Reference Toxicant data
Great Lakes Environmental Center
2002-2009
D 9 ^
^ * ~ - - ~ ~ g—~ ~ * ~ ~ ~ ~ ~ ~ | ~ ~ ~ ~ ~—~ - ~ ~ ¦ ~ ~ ~
i 8 A A
f . ¦ / \ / ' ¦ V \
sfi "7 B-* -"m \ M
e 7 - " \ / v ' '
C3 \ / '
W \ /
» 6 - ¦
o
/ X / \
\ \ I
\ / \ / I
/
Test Date
LC50 ^ Mean +2 std Mean -2 std
-------
Statistical Analyses
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
nitrate C. dilutus 48-hr acute
Proportion
Observed
Responding
Predicted
Number
Number
Proportion
Adjusted for
Proportion
Cone.
Exposed
Resp.
Responding
Controls
Responding
402.5000
20
0
0.0000
0.0000
0.0055
734.0000
20
3
0.1500
0.1500
0.1197
1131.5000
20
9
0.4500
0.4500
0.4236
1647.5000
20
13
0.6500
0.6500
0.7458
2750.0000
20
20
1.0000
1.0000
0.9661
Chi - Square for Heterogeneity (calculated)
Chi - Square for Heterogeneity
(tabular value at 0.05 level)
2. Oil
7 .815
Mu
Sigma
3,090485
0.191079
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept
Slope
•11.173868
5.233441
2.816005
0.909781
•16.693237,
3.450270,
•5.654499)
7.016612)
Theoretical Spontaneous Response Rate =
0.0000
-------
nitrate C, dilutus 48-hr acute
Estimated LC/EC Values and Confidence Limits
Exposure 95% Confidence Limits
Point Cone. Lower Upper
LC/EC
1
00
442-566
253
503
590.752
LC/EC
5
00
597.281
395
184
746.780
LC/EC
10
00
700.806
498
896
849.292
LC/EC
15
00
780.639
582
352
928.738
LC/EC
50
00
1231.642
1055
794
1437.438
LC/EC
85
00
' 1943,205
1632
436
2608.678
LC/EC
90
00
2164.568
1784
989
3045.324
LC/EC
95
00
2539.745
2029
853
3844.837
LC/EC
99
00
3427.609
2565
778
5994.146
-------
nitrate C. dilutus 48-hr acute
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10 +
9 +
8 +
7 +
6+ ....
.... O
5 +
. o. .
4+ o....
3 +
2 +
1+
-------
Appendix 4: Raw Data for 96-hr Toxicity of
Nitrate on Amphinemura delosa
-------
Raw Laboratory Data
-------
96 Hour Water Column Acute Test Data Sheet/ 6 treatments
fsPcclcs: A cJoS SVW'o^ \Z&\ry, {,Co *.
StartDate/Time: ^/Qq Age: uvyV^wg^w
[Initials: | P T p <
Temp/Light: \%? c 1» f
Glassware:
Survival
_
Treatment
rep
Oh
24 h
48 h
72 h
96 h
Cbem Oh
24h
48h
72h
96h
A
Ccevt*o I
1
M
1
H
H
temp
fZ-' v
ft.3
iZ-'l
IT., f
2
i1
uf
1
f
pH
***?
1
-?. Sr
It
3
H
H
H
H
cond
%Ht
7*1 "7
O-iT
3 00
zis
4
i
H
t
V
f
D.O.
8-?
1 zsr
Alk
0 0
X
X
X
10
Hard
30
X
X
X
¦•¦>¦
'B?
IHi
t fc'2-5")
1
44
H
If
1
M
temp
f7-5
13-<4
iZ.4
( 1 s"
1 %*«
2
jU|-
?
-2.
3
pH
r-o
•7^
*7 ,*f
7 ^
"1 . 1
3
14
•z
3,
3
cond
IHHO
< VST'
11S?
/itaO
/W/
4
_i=.
Z-
cond
2S~f 0
'i-^0
Z-STjO
Z-SToo
Z^TlO
4
«j
Lj
X
1
1
D.O.
1-G*\
Q/7 2-
Alk
U°
X
X
G> Z
Hani
*7X-
X
X
X
IV
D
5b H
(s^y
1
H
H
3
3
"i-
temp
jz: —
"•1
| 1L' S
~ /1-8T
2
V
?
i
(
s
PH
-ir.0
-7
"1-f
1 -i
7-1
3
H
-f
3
2
cond
HO*! 0
Hfcfo
HICO
IllO
%s.rb
4
H
M
3
i
J
D.O.
9 "ST
Alk
t.n
X
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X
t 0
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12-
X
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X
1x-
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1
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2-
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temp
it'S
II,"I
n.
9nt3
¦-. 7v
?-7^o
te?0
4
t™|
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1
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D.O.
°7
Alk
1-0
X
X
X
fe z.
Hard
X
X
X
...9 ?
1
¦4
O
<0
O
O
temp
i-x-r
11 0
1 z-H
1?-^
/z-S-
F
2
Lf.
I
¦0
0
0
pH
7-1
1,1
1-1
1
3
H
2.
o
0
0
cond
IM.WO
ft. 2-70
1 ttlo
1 (.Z£C
Itl'io
>7-"5 °
4
z-
0
3
0
D.O.
^•Of
^11
Alk
fco
X
X
X
iff 0
Hard
X
X
X
11-
Initials
05
0S
Of
OS
c>S
-------
Analytical Chemistry Data
-------
Dave Soucek, INHS
Nitrate in water
Analyst: Duarte Kimme
ABE Water Quality Lab
A. dolosa
EPA 353,1
EPA 354.1
C-D
Sample ID
Date
M03+N02 as N mg/L
N02-N mg/L
N03-N mg/L
AN03
5/18/2009
-------
Statistical Analyses
-------
CT-TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS
SPEARMAN-KARBER
TRIM: 31.25%
LC50: 1752.739
95% LOWER CONFIDENCE: 1393.893
95% UPPER CONFIDENCE: 2203.966
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
152.00 16. 2. 12.50 .2090D+00
274.00 16. 1. 6.25 .2594D-01
596.00 16. 1. 6.25 .2594D-01
1230.00 16. 4. 25.00 .3841D+01
2286.00 16. 11. 68.75 .1051D+02
THE BINOMIAL TEST SHOWS THAT 596.00 AND +INFINITY CAN BE USED AS
STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9741 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 1757.770
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
1 .641 1757.77 1247.63 2929.55
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
5 .309 1.00 .05
SLOPE = 1.54
95% CONFIDENCE LIMITS: .69 AND 2.40
LC50= 2112.74
95% CONFIDENCE LIMITS: 1240.50 AND 8125.13
LCI = 65.65
95% CONFIDENCE LIMITS: 2.45 AND 177.99
DATE: May 14, 2009 TEST NUMBER: DURATION: 24 h
SAMPLE: N-N03 SPECIES: Amphinemura delosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL ******* 595 000 ******* *******
MA A ******* ******* ******* *******
******* ******* ******* *******
SPEARMAN ******* ******* ******* gio 073
NOTE: MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING.
ADJUSTMENTS WERE MADE PRIOR TO SPEARMAN-KARBER ESTIMATION.
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 12.50%
LC50: 584.194
95% LOWER CONFIDENCE: 409.148
95% UPPER CONFIDENCE: 834.130
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
152.00 16. 2. 12.50 .2090D+00
274.00 16. 5. 31.25 .1051D+02
596.00 16. 6. 37.50 .2272D+02
1230.00 16. 13. 81.25 .1064D+01
2286.00 16. 16. 100.00 .1526D-02
THE BINOMIAL TEST SHOWS THAT 152.00 AND 1230.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 98.7274 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 726.832
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
4 .104 532.67 387.14 716.62
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
6 .132 1.00 .30
SLOPE = 2.43
95% CONFIDENCE LIMITS: 1.55 AND 3.32
LC50= 523.39
95% CONFIDENCE LIMITS: 372.20 AND 726.53
LCI = 57.81
95% CONFIDENCE LIMITS: 15.15 AND 111.40
DATE: May 14, 2009 TEST NUMBER: DURATION: 48 h
SAMPLE: N-N03 SPECIES: Amphinemura delosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 726.832 152.000 ******* *******
MAA 532.665 387.139 716.617 329.477
PROBIT 523.386 372.204 726.532 354.328
SPEARMAN 584.194 409.148 834.130 424.981
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 12.50%
LC50: 498.666
95% LOWER CONFIDENCE: 352.223
95% UPPER CONFIDENCE: 705.995
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
152.00
16.
2.
12.50 .2090D+00
274.00
16.
6.
37.50 .2272D+02
596.00
16.
7.
43.75 .4018D+02
1230.00
16.
14.
87.50 .2090D+00
2286.00
16.
16.
100.00 .1526D-02
THE BINOMIAL TEST SHOWS THAT 152.00 AND 1230.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.5819 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 655.097
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
4 .104 468.58 332.66 626.41
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
6 .132 1.00 .34
SLOPE = 2.51
95% CONFIDENCE LIMITS: 1.60 AND 3.43
LC50= 457.94
95% CONFIDENCE LIMITS: 325.07 AND 628.88
LCI = 54.29
95% CONFIDENCE LIMITS: 14.47 AND 103.69
DATE: May 14, 2009 TEST NUMBER: DURATION: 72 h
SAMPLE: N-N03 SPECIES: Amphinemura delosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 655.097 152.000 ******* *******
MAA 468.582 332.655 626.409 293.753
PROBIT 457.939 325.067 628.876 303.808
SPEARMAN 498.666 352.223 705.995 353.772
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 12.50%
LC50: 456.456
95% LOWER CONFIDENCE: 324.657
95% UPPER CONFIDENCE: 641.760
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
152.00 16. 2. 12.50 .2090D+00
274.00 16. 6. 37.50 .2272D+02
596.00 16. 8. 50.00 .5982D+02
1230.00 16. 15. 93.75 .2594D-01
2286.00 16. 16. 100.00 .1526D-02
THE BINOMIAL TEST SHOWS THAT 152.00 AND 1230.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.7650 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 596.000
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
4 .104 424.69 294.84 567.40
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
6 .130 1.00 .41
SLOPE = 2.75
95% CONFIDENCE LIMITS: 1.76 AND 3.74
LC50= 419.96
95% CONFIDENCE LIMITS: 303.48 AND 566.61
LCI = 59.90
95% CONFIDENCE LIMITS: 18.06 AND 108.43
DATE: May 14, 2009 TEST NUMBER: DURATION: 96 h
SAMPLE: N-N03 SPECIES: Amphinemura delosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 596.000 152.000 ******* *******
MAA 424.686 294.835 567.401 272.566
PROBIT 419.959 303.483 566.612 263.129
SPEARMAN 456.456 324.657 641.760 317.103
**** = LIMIT DOES NOT EXIST
-------
Appendix 5: Raw Data for 96-hr Toxicity of
Nitrate on Sphaerium simile
-------
Raw Laboratory Data
-------
961 lour Water Column Acute Test Data Sheet
Toxicant: j-4n5 |Speci.es: *&•
Specie
"A
Rate: 13 ."1 tOM*/7,1-
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Culture: Crfe*. i )L ¦ CoHt-C*^ I? 'i. y -O6!
Start Date/Time: T-(a<[r.-\/^t$iw
Temp: 2-P C I
Age: v^vs^p >..-o vtwJj^ Initials:
Light: i {p 11
Survival
I
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m
-------
Analytical Chemistry Data
-------
Dave Soucek. INHS
N in Water
Analysis Date: 07/28/09
Analyst: Duane Kimme
ABE Water Quality Lab
nple ID
Date
Location
NOrN mg L
N03+N02 as N mg L
N0j.N mg. L
A
07/21/09
In
«MDL
-------
Statistical Analyses
-------
CT-TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS
MINIMUM REQUIRED TRIM IS TOO LARGE: 100.0,SO SK IS NOT CALCULABLE.
SPEARMAN-KARBER
TRIM: .00%
LC50: .000
95% CONFIDENCE LIMITS
ARE UNRELIABLE.
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
65.00 20. 0. .00 .9537D-04
132.00 20. 0. .00 .9537D-04
272.00 20. 0. .00 .9537D-04
569.00 20. 0. .00 .9537D-04
1125.00 20. 0. .00 .9537D-04
THE BINOMIAL TEST SHOWS THAT 1125.00 AND +INFINITY CAN BE USED AS
STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9999 PERCENT.
THE LC50 FOR THIS DATA SET IS GREATER THAN 1125.00
WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT
WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER
THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE
ANY STATISCALLY SOUND RESULTS.
DATE: July 21, 2009 TEST NUMBER: DURATION: 24 h
SAMPLE: nitrate SPECIES: Sphaerium simile
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL ******* ******* ******* *******
MA A ******* ******* ******* *******
******* ******* ******* *******
SPEARMAN 000 ******* ******* *******
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 15.00%
LC50: 652.369
95% LOWER CONFIDENCE: 507.670
95% UPPER CONFIDENCE: 838.310
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
65.00 20. 0. .00 .9537D-04
132.00 20. 0. .00 .9537D-04
272.00 20. 0. .00 .9537D-04
569.00 20. 8. 40.00 .2517D+02
1125.00 20. 17. 85.00 .1288D+00
THE BINOMIAL TEST SHOWS THAT 272.00 AND 1125.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.8711 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 655.578
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
2 .086 665.63 555.56 823.73
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
7 .172 1.00 .85
SLOPE = 5.28
95% CONFIDENCE LIMITS: 3.09 AND 7.47
LC50= 679.48
95% CONFIDENCE LIMITS: 557.54 AND 832.89
LCI = 246.28
95% CONFIDENCE LIMITS: 116.74 AND 343.21
DATE: July 21, 2009 TEST NUMBER: DURATION: 48 h
SAMPLE: nitrate SPECIES: Sphaerium simile
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 655.578 272.000 ******* 853.000
MAA 665.629 555.558 823.727 268.168
PROBIT 679.477 557.544 832.892 275.348
SPEARMAN 652.369 507.670 838.310 330.640
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 5.00%
LC50: 385.838
95% CONFIDENCE LIMITS
ARE UNRELIABLE.
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
65.00 20. 1. 5.00 .2003D-02
132.00 20. 1. 5.00 .2003D-02
272.00 20. 1. 5.00 .2003D-02
569.00 20. 20. 100.00 .9537D-04
1125.00 20. 20. 100.00 .9537D-04
THE BINOMIAL TEST SHOWS THAT 272.00 AND 569.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9979 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 374.786
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
4 .066 322.86 253.34 421.36
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
9 5.861 18.95 .00
A PROBABILITY OF 0 MEANS LESS THAN 0.001
SLOPE = 4.34
95% CONFIDENCE LIMITS: -6.16 AND 14.83
LC50= 331.89
95% CONFIDENCE LIMITS: 0 AND + INFINITY
LCI = 96.47
95% CONFIDENCE LIMITS: 0 AND + INFINITY
DATE: July 21, 2009 TEST NUMBER: DURATION: 72 h
SAMPLE: nitrate SPECIES: Sphaerium simile
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 374.786 272.000 569.000 297.000
MAA 322.857 253.340 421.357 168.017
PROBIT 331 892 ******* ******* *******
SPEARMAN 385 838 ******* ******* *******
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 5.00%
LC50: 370.656
95% LOWER CONFIDENCE: 322.509
95% UPPER CONFIDENCE: 425.990
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
65.00 20. 1. 5.00 .2003D-02
132.00 20. 2. 10.00 .2012D-01
272.00 20. 1. 5.00 .2003D-02
569.00 20. 20. 100.00 .9537D-04
1125.00 20. 20. 100.00 .9537D-04
THE BINOMIAL TEST SHOWS THAT 272.00 AND 569.00 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9979 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 374.786
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
4 .066 307.10 240.65 398.53
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
8 2.889 9.97 .00
A PROBABILITY OF 0 MEANS LESS THAN 0.001
SLOPE = 4.00
95% CONFIDENCE LIMITS: -2.80 AND 10.79
LC50= 319.56
95% CONFIDENCE LIMITS: 0 AND + INFINITY
LCI = 83.66
95% CONFIDENCE LIMITS: 0 AND 260.53
DATE: July 21, 2009 TEST NUMBER: DURATION: 96 h
SAMPLE: nitrate SPECIES: Sphaerium simile
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 374.786 272.000 569.000 297.000
MAA 307.101 240.645 398.531 157.886
PROBIT 319 557 ******* ******* *******
SPEARMAN 370.656 322.509 425.990 103.482
NOTE: MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING.
ADJUSTMENTS WERE MADE PRIOR TO SPEARMAN-KARBER ESTIMATION.
**** = LIMIT DOES NOT EXIST
-------
Appendix 6: Raw Data for 96-hr Toxicity of
Nitrate on Hyalella azteca
-------
Raw Laboratory Data
-------
GleC
PROJECT NUMBER:
TEST SPECIES: I
INVESTIGATORS:
TEST MATERIAL: N t"Vt^XjO
Utc>.Q
96-HOUR STATIC ACUTE TOXICITY TEST
(Had
TYPE OF TEST:
DILUTION WATER:
lTot-<
NUMBER OF ORGANISMS/CHAM BER:_
NUMBER OF CHAMBERS:
s.
GLC AND/OR BATCH NUMBER:
TEST TEMPERATURE:
3DttJ
DATE
TEST
OAY
TECH,
INITIALS
TREATMENT LEVEL
CONTROL
(31- L
^o^U-
TIME
REPUCATE NUMBER
1
2 3 4
1 2 | 3 4
1 2)3 A
t
2
3
4
1
2
3
4
1
1
2
4
€
0
TEMPERATURE
oa,o
pa-a
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. «3p.O
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pH
^•00
-1T-0?
Y'i/O
K.on
DO (mgfLj
Y'i
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$
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NUMBER LIVE
5
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5
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5
5
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5
6
s
5
5
•3>
%
6
s
$
5
5
s
5
H
§
OBSERVATIONS
tf
If-
2-P
1?
a
if
lr
pH
7.*\%
. —1 °>s
• "JT'OO
DO fmgA.)
TS
• *S-<^
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TEMPERATURE TO
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if
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OBSERVATIONS
/F
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pH
<=? "Z-6
- *7
. f.^Z_
¦
t>-
33, >
S3. ^
OBSERVATION KEV;
DOB • Oried out on Beaker
F - Floater FS-FSIm on Surface
PM - Particulate Matter
DAY 0: CONTROL
HARDNESS
DAY 4t CONTROL
ISND (mis):
START(mUS:
St
DAY 0: HIGH CONCENTRATION
fcpMUu
ALKAUHSTY
Mil
T~
"20 O
91,0
DAY 4: HIGH CONCENTRATION
.QOfftftU*
*Vt
7'0
5.0
RE"EWEDB^ jpOftllu u)
Ml
DATE:
-------
''iri3.1t Enviw
PROJECT NUMBER:_
TEST SPECIES:
TEST MATERIAL:
fr\»Tiafcj
INVESTIGATORS:
96-HOUR STATIC ACUTE TOXICITY TEST
PiUJTION WATER: ^&A ArWfc
TYPE OF TEST:_
NUMBER OF ORGANISMS/CHAMBER: _
NUMBER OF CHAMBERS:^
AGE OF ORGANISMS:
S \ ¦¦"V "Co r /-J
^ f\ -for rkfrmS
m
noM
GLC AND/OR BATCH NUMBER:
_2£LV
c.
TEST TG M P ERATU RE
INCUBATOR#: pCL PHOTOPERIOD: Y
DATE
TEST
DAY
TECH.
INITIALS
TREATMENT LEVEL
CONTROL ^
lOO^i^
TIME
REPLICATE NUMBER
12/34
i -
3 4
1
2
3
4
1
2 3
4
1
2
3 4
1
2 3 4
a
TEMPERATURE
/
Sa
V\\
%
1
NUMBER LIVE
i
3
5
H
H
OBSERVATIONS
/
it
i?
It
*
-------
Analytical Chemistry Data
-------
r^T
VjrLbv^
K ^ ^
.at Lakes Environmental Center
PREPARATION OF RECONSTITUTED WATER
WATER TYPE: /hfyfj /4&fJ
BATCH NUMBER;
P&4
DATE PREPARED: *B 1*5}t>^
TOTAL VOLUME: L
DATA BY:
/3Q
SALTS
AMOUNT ADDED TO FINAL VOLUME
NOTES
NaHCO,
O 0s
CaSOR * 2H?0
i,
MgSO,
i .35
KCI
o • o%
WATER SOURCE:
RECONSTITUTED HARD WATER - WATER QUALITY
Date: |(^|oC? Checked By: f\
I.MLr
J*L
ay
SPECIFIC CONDUCTANCE
3^
TOTAL HARDNESS
SAMPJ.E VOLUME
' IS
DILUTED TO
31
mL TITRANT USED
n
mall CaCO,
TOTAL ALKALINITY
SAMPLE VOLUME
Sx_
DILUTED TO
mL TITRANT USED
mg/L CaCO,
Hardness: * Alkalinity: / y
End mis: \ J. 5 End mis: u * "
Start mis:. Start mis:. 33.
7-
-------
PROJECT#: 6112-12
Nitrate Nitrite Tox
CALIBRATION
CONCENTRATIONS
(ppm)
0.5
1
10
25
50
Date
Test Day
Natural
log
-0.6931472
0
2.30258509
3.21887582
3.91202301
Regression Output:
Constant
Std Err of Y Est
R Squared
No. of Observations
Degrees of Freedom
08/06/2009
0
Millivolts
-53.0
-62.9
-119.8
-141.2
-159.8
-66.194866
2.819348
0.997345
5
3
tox test
X Coefficients)
Std Err of Coef.
-23.537499
0.70114936
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
H. azteca
1 100m.fi/LN03
0
water
3.5
-2.9610141
0.0517664
-0.0960736
H. azteca
1 lOmg/L N02
0
water
5.4
-3.0417363
0.0477519
-0.1000881
FHM
13000 mg/L N03
0
water
14.4
-3.4241049
0.03257843
-0.1152616
| Blank j
0
-21.2
-1.9116248
0.14783999
0
-------
Nitrate Day 0 Hyalella azteca Analytical Results 8.6.09
LabSamplelD
SampieDescription
ParamID
Results Units
RL LabQualMer
AnalysisMethod
AnafysisDate
SampleOate Analyst
Calibration Date
Calibration Person
HB08Q60107
Mod. Hand Corrtrol-nltrate/nitrite
N03
<0.0053 mg/L
0.022135 U
A4500F
8/7/2009
8
0
1
8/7/2009
Ben Cook
HB08Q60107
Mod. Han) Control-nitrate/nitrite
N02
<0.0036 mg/L
0.016425 U
A4500B
8/6/2009
8/8/2009 Jl
8/6/2009
Jl
HB08080198
Stock 1,4g/l-nitrate
N03
1157 mg/L
44,27
A4500F
8/7/2009
8/8/2009 Ben Cook
8/7/2009
Ben Cook
HB08060198
Stock 1,4g/L-nltiate
N02
<0.0036 mfl/L
0.016425 U
A45Q0B
8/8/2009
8/3/2009 Jl
8/6/2009
Jl
HB08060199
100mf/L-nitrste
N03
121.5 mfl/L
4.427
A4500F
8/7/2009
8/8/2009 Ben Cook
8/7/2009
Ben Cook
HB08060199
100mg/L-nitrate
N02
<0.0036 mfl/L
0.016425 U
A4S00B
8/8/2009
8/8/2009 Jl
8/6/2009
Jl
HB08060200
60mg/L-nitrate
N03
68.7 mfl/L
4.427
A4500F
8/7/2009
8/8/2009 Ben Cook
8/7/2009
Ben Cook
HB08060200
eOmg/L-rtftrate
N02
<0.0036 mg/L
0.016425 U
A4500B
8/6/2009
8/9/2009 Jl
8/6/2009
Jl
HB08060201
38mg/L-nitrate
N03
43.2 mg/L
2,2135
A4500F
8/7/2009
8/8/2009 Ben Cook
8/7/2008
Ben Cook
HB08060201
36mg/L-nitrate
N02
<0.0036 mg/L
0.016425 U
A4500B
8/6/2009
8/6/2009 Jl
8/6/2009
Jl
HB080602C2
21.8mg/L-nitrate
N03
25.14 mfl/L
1.10675
A4500F
8/7/2009
8/8/2009 Ben Cook
8/7/2009
Ben Cook
HBOS060202
2l,8mfl/L-nitrate
N02
<0.0036 mg/L
0.016425 U
A4500B
8/6/2009
8/6/2009 Jl
8/6/2009
Ji
HB08060203
12,96mg/L-nltrate
N03
12.08 mg/L
1.10675
A4500F
8/7/2009
8/6/2009 Ben Cook
8/7/2009
Ben Cook
HB08060203
12.86 mg/L-nitrate
N02
<0.0036 mg/L
0.016425 U
A4500B
8/8/2009
8/6/2009 Jl
8/6/2009
Jl
HB08060204
7.78mg/L-nHrate
N02
<0.0036 mg/L
0,016425 U
A4S0CB
8/8/2009
8/6/2009 Jl
8/6/2009
Jl
HB08060204A
7.78mg/L-nltrate
N03
9.43 mg/L
0,4427
A4500F
8/7/2009
8/6/2009 Ben Cook
8/7/2009
Ben Cook
HB08060204B
7.78mg/L-nitrate
N03
9.57 mfl/L
0.4427
A450OF
8/7/2009
8/6/200B Ben Cook
8/7/2009
Ben Cook
Page 1
-------
Nitrate Day 4 Hyalella azteca Analytical Results 8 10 09
LabSamptelD
Sample Description
ParamID
Results Units
RL LabQualifier
AnalysisMethod
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Person
H608110254A
Mod. Hard Control-nitrate/nitrite
N03
0.248
mg/L
0.08854
A4500F
8/13/2009
8/11/2003 Ben Cook
B/13/2008
Ban Cook
HB08110254A
Mod, Hard Control-nitrate/nitrite
N02
0.0659
mg/L
0.016425
A4500B
8/12/2009
8/11/2009 Jl
8/12/2009
Jl
HB08110254B
Mod, Hand Control-nitrate/nitrite
N03
0.188
mg/L
0.08854
A4500F
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB08110254S
Mod, Hard Control-nitrate/nitrite
N02
0.0684
mg/L
0.016425
A4500B
8/12/2009
8/11/2009 Jl
8/12/2009
Jl
HB08110255
100mg/L-nitrate day 4
N03
107.2
mg/L
3.5418
A4S00F
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB08110255
lOOmg/L-nitrate day 4
N02
0.0437
mg/L
0.016425
A4500B
8/12/2009
8/11/2009 Jl
8/12/2009
Jl
HB08110256
SOmg/L-nitrate day 4
N03
65.8
mg/L
2.2135
A4500F
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB08110258
60mg/L-nfcate day 4
NOS
1.348
mg/L
0.16425
A4500B
B/12/2009
8/11/2009 Jl
8/12/2009
Jl
HB08110257
36mg/l-nitrate day 4
NOS
37.7
mg/L
2.2135
A4500F
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB08110257
36mg/L-nitrate day 4
N02
0.0205
mg/L
0.016425
A4500B
8/12/2009
8/11/2009 Jl
8/12/2006
Jl
HB08110258
21.6mg/L-nllnte day 4
N03
25.56
mg/L
0.8854
A4500F
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB08110258
21.6mg/L-nftrato day 4
N02
0.0168
mg/L
0.016425
A4500B
8/12/2009
8/11/2009 Jl
8/12/2009
Jl
HB08110259
l2.96mg/L-nitrate day 4
N03
15.06
mg/L
0.88405
A4500F
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB08110259
12,96mg/l-nitrate day 4
N02
0.0221
mg/L
0.018425
A4500B
8/12/2009
8/11/2009 Jl
8/12/2009
Jl
HB08110280
7.78mg/L-nitrate day 4
N03
9.508
mg/L
0,4427
A4500F
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB08110260
7.78mg/L-nitrate day 4
N02
0.0206
mg/L
0.016425
A4500B
8/12/2009
8/11/2009 Jl
8/12/2009
Jl
Page 1
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
J ..(,3
GleC
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
. . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8.6.09
Mailee Garton/Jamie Saxton
in. garton
Bottle ID
Date /Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
{Site information /
sample type)
Preservation
Check the requested analysis (see definitions below1
' 1
Alkalinity
O
U
Chlorophyll a
Hardness
Z
SB
z
1
.O
Z
K)
O
- 'Z
Uj
Ck
f-K
' H
(L.
H
f
Nitrate
Nitrite
HAN03W
-00-01
8/6/09
125 niL
HOPE
Mod. Hard Control -
nitrate/nitrite
h2so4
V-
!. .J- ;:i
¦ 'J4-'
X ¦:
HAN 03 W
-00-02
8/6/09
125 mL
HDPE
Mod. Hard Control -
nitrate/nitrite
Cold
SiSSt
X
HAN03W
-00-03
8/6/09
125 mL
HDPE
Stock 1.4 g/L - nitrate
h2so4
¦ - - -;¦¦¦¦¦*
;
x ¦
b'
if.'f - •
HAN 03 W
-00-04
8/6/09
125 mL
HDPE
Stock 1.4 g/L - nitrate
Cold
¦v:;
WviV*>!
X
k;,.'..
HAN03W
-00-05
8/6/09
125 mL
HDPE
100 mg/L- nitrate
h2so4
~ -
r
W", l[
HAN03W
-00-06
8/6/09
125 mL
HDPE
60 mg/L - nitrate
h,so4
X:
:rr- 1
i"-- ¦ "• .'
Shipper:
Date/Time:
Receiver:
D(/f
Date/Time:
3
Temperature:
Trnr.
Definitions
BODj = Biochemical Oxjien Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj -N = Ammonia-Nitrogen TKN = Total Kjetdahl Nitrogen TSS= Total Suspended Solids
NO1/NO2-N = Nitrate/Nitrite-Nitrogen
fdtfteF frequently requested analyses
NOi-N = Nitrite Nitrogen
NQs -N = Nitrate Nitrogen
Total N - Total Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
739 Hastings St
Traverse City, MI 49686
Phone: 231-941-2230
Fax:231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page.
of ffS
8.6.09
Mailee Garton/Jamie Saxton
m. garton
Check the requested analysis (see definitions below)
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
*iS.~
' -Is*
it.
in
0
O
a
Chloride
j Chlorophyll a
- j-' '
Hardness
z
i
X
z
z
0
z
T0S
: H
H
TSS
Nitrate
¦ , -V 1 l";."
Nitrite
HAN03W-
00-07
8/6/09
125 mL
HDPE
36 mg/L - nitrate
H2SO4
'Q
X\-
HAN03W-
00-08
8/6/09
125 mL
HOPE
21.6 mg/L - nitrate
h2so4
i.
X'.">
HAN03W-
00-09
8/6/09
125 raL
HDPE
12.96 mg/L - nitrate
h2so4
1
-i S
HAN03W-
00-10
8/6/09
125 raL
HDPE
7.78 mg/L - nitrate
h3so4
X '
HAN03W-
00-11
8/6/09
125 mL
HDPE
100 mg/L - nitrate
Cold
'
" iii.*' :
X
HAN03W-
00-12
8/6/09
125 mL
HDPE
60 mg/L - nitrate
Cold
' '.i
Y-»-
X
Shipper: Date/Time: Receiver: Date/Time: Temperature:
Definitions
BODj = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NH, -N = Ammonia-Nitrogen TKN - Total Kjeldah! Nitrogen TSS = Total Suspended Solids
N0j/N02-N = Nitrate/Nitrite-Nitrogen
Abbreviations for other frequently reumattd analyses
N02 -N = Nitrite Nitrogen Total N = Total Nitrogen
NO; -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
739 Hastings St.
Traverse City, MI 496S6
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
3 »f3
8.06.09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis {see definitions below'
1
Alkalinity
Q
O
03
Chloride
Chlorophyll a
•-W .
_>
SB
Z
£ •
o
z
TDS
TKN
SLi
H
TSS
Nitrate
Nitrite
HAN03W-
00-13
8/6/09
125 mL
HDPE
36 mg/L - nitrate
Cold
v.
X
HAN03W-
00-14
8/6/09
125 mL
HDPE
21.6 mg/L - nitrate
Cold
X
HAN03W-
00-15
8/6/09
125 mL
HDPE
12.9 mg/L — nitrate
Cold
-
X
HAN03W-
00-16
8/6/09
125 mL
HDPE
7.8 mg/L - nitrate
Cold
X
Shipper:
Date/Time:
L £
2.
V
BODj = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NOj/NOj -N ** Nitrate/Nitritc-Nitrugeii
Definitions
I DS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
Receiver:
/1J^
Date/Time:
1^"Q1
Temperature:
TP = Total Phosphorus
TSS = Total Suspended Solids
Ablirfviations for other freq iientlv requested analyses
NO, -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP — Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.g1ec.com
Page
***» ^jioh0!
of 3
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person: Mailee Garton/Jamie Saxton
Email Address:
Phone Number:
Collector:
m. garton
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
:f:
•: m
r "
BOD,
Chloride
Chlorophyll a
.5
z,
2
SSI
. ..-.'SM. .
•O
O
z
&
a
¦
a,
H
' CO
Cfi
H
ss-.
I®
1
z
HAN03W
-04-01
S/JMT/09
%o
125 mL
HDPE
Mod. Hard Control -
nitrate/nitrite
h2so4
V - '
X -
HAN03W
-04-02
8/R/09
125 mL
HOPE
Mod, Hard Control -
nitrate/nitrite-day 4
Cold
¦
X
'
-J ]"ij' ' I
X-
I
z-..
X
HAN03W
-04-03
8/W7Q9
io
125 mL
HDPE
100 mg/L - nitrate
day 4
H1SO4
Tp:—
X
HAN03W
-04-04
%/yfm
(O
125 mL
HDPE
60 mg/L - nitrate day
4
H2SO4
,
X
Shipper:
Date/Time:
Receiver:
ft.. M
Date/Time:
"S -/t-oT
Temperature:
Definitions
BODs = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj -N - Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NOj/KOi -N = Nitrate/Nitrite-Nitrogen
Abbreviations for otBeMred^
NOj -N = Nitrite Nitrogen Total N - Total Nitrogen
NO) -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
G ,
LEC
Page ^ of 3
c.M i'.
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
:.com
Great Lakes Environmental Center
Facility:
, . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysts (see definitions below)
t
*n
Q
2
Chloride
Chlorophyll a
' '^1
Hardness
Z
<*3
S3
z
O
5
- - n
O
. 'K
m
Q
-f-1
H
00'.';..
CO
'H
I
C' :
X
Shipper:
Date/Time:
Receiver:
Date/Time:
8 '(('0 1
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NOj/NOj -N *¦ Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently i
NO.-N -NitriteNitrogen Total N = Total Nitrogen
NOj -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
739 Hastings St
Traverse City, MI 49686
Phone:231-941-2230
Fax:231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Page
of
3_
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis {see definitions below)
j
Q
i
Chloride
J Chlorophyll a
¦5
¦U.
M
m
%
<*>
S
Z
¦% ¦
' rO-
\ %
• .#
'Z
so
e
a*
w
H ;
Nitrate
'u
• MM
z
HAN03W-
04-11
8/^/09
125 mL
HDPE
36 mg/L — nitrate day
4
Cold
X
HAN03W-
04-12
8//1/09
fo
125 mL
HDPE
21.6 mg/L - nitrate
day 4
Cold
X
HAN03W-
04-13
8/.KI/09
125 mL
HDPE
12.9 mg/L — nitrate
day 4
Cold
- .
i*- y."s -
X
HAN03W-
04-14
i/y/09
125 mL
HDPE
7,8 mg/L - nitrate day
4
Cold
r -
X
;h •• ¦
• •
Shipper: Date/Time: Receiver: Date/Time: Temperature:
L JT§2lSjU Mojo^^ ^^
Abbreviations for other frequently requested analyst
NOi-N = Nitrite Nitrogen TotalN = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
- - . - - 1 i - . . 1 1
Definitions
BODj •= Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS - Total Suspended Solids
NO5/NO2 -N = Nitrate/Nitrite-Nitrogen
-------
Reference Toxicant Data
-------
Greot Lakes Entflronriienlal Center
Hyalella azteca Survival
NaCl Reference Toxicant data
2002-2009
U
«¦
z
~ ~ ~
~ ~—~ - ~ -
a 4.5
~ ~ ~ # ~ ~ ~ ~ ~ ~ ~
p_^
«
OH
O
VI
U
J
\ /
\ /
\ /
Mi
/
~ ~—ft—*- ~
3.5
A A A A A A A A B A AAAAAAA~"AAAAAAAAAA-~A-AAAA
l I I i I I I I I I I I I I I i i i I I I ! I i I I I I I I I L
S> <;
cfi °
—I ! i I 11 1 I I I I I I I 1 1 1 I 1 i I 1 1 1 I I I I 1
O0^ ^ ^ ^ ° ^ S* ^ -s# cf-
Test Date
-¦- LC50 * Mean +2 std ~+~ Mean -2 std
-------
Statistical Analyses
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1,5
Nitrate H azteca 96 hour acute 48 hour
Cone.
Number
Exposed
Number
Reap.
Observed
Proportion
Responding
Proportion
Responding
Adjusted for
Controls
Predicted
Proportion
Responding
9
5000
20
0
0
0000
0
0000
0
0003
14
0100
20
0
0
0000
0
0000
0
0018
25
3500
20
0
0
0000
0
0000
0
0169
40
4500
20
3
0
1500
0
1500
0
0673
67
3000
20
2
0
1000
0
1000
0
2079
114
3500
20
10
0
5000
0
5000
0
4590
Chi - Square for Heterogeneity (calculated)
Chi - Square for Heterogeneity
(tabular value at 0,05 level)
4 .114
9.488
Mu
Sigma
2.091584
0.323926
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept
Slope
-1.456972
3.087121
1.499863
0.807131
-4 .396704,
1.505145,
1.482759)
4 .669097)
Theoretical Spontaneous Response Rate = 0.0000
-------
Nitrate H azteca 96 hour acute 48 hour
Estimated LC/EC Values and Confidence Limits
Exposure 95% Confidence Limits
Point Cone. Lower Upper
LC/EC
1
00
21
779
6
039
34
214
LC/EC
5
00
36
204
16
499
49
704
LC/EC
10
00
47
472
27
475
62
241
LC/EC
15
00
56
999
37
760
74
373
LC/EC
50
00
123
476
91
632
249
424
LC/EC
85
00
267
486
161
598
1151
046
LC/EC
90
00
321
165
183
408
1665
452
LC/EC
95
00
421
124
220
804
2884
862
LC/EC
99
00
700
058
311
497
8115
786
-------
Nitrate H azteca 96 hour acute 48 hour
PLOT OF ADJUSTED PROSITS AND PREDICTED REGRESSION LINE
Probit
10 +
3+
7 +
6+
5+ O.
4 +
3 +
2 +
O+o o
EC01 EC10 EC25 EC50 EC75 EC90
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
Nitrate H azteca 96 hour acute 72 hour
Proportion
Observed
Responding
Predicted
Number
Number
Proportion
Adjusted for
Proportion
Cone.
Exposed
Resp.
Responding
Controls
Responding
9.5000
20
0
0.0000
0.0000
0.0022
14.0100
20
1
0.0500
0.0500
0.0085
25.3500
20
0
0.0000
0.0000
0.0474
40.4500
20
3
0.1500
0.1500
0.1339
67.3000
20
4
0.2000
0.2000
0 .3103
114.3500
20
13
0,6500
0.6500
0 .5570
Chi - Square for Heterogeneity (calculated)
Chi - Square for Heterogeneity
{tabular value at 0.05 level}
6.977
9 .488
Mu
Sigma
2.006560
0.360678
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept
Slope
-0.563300
2.772556
1.104681
0.614797
-2.728474,
1,567554,
1.601874)
3 .977557)
Theoretical Spontaneous Response Rate
— 0 . 0 0 0 Q
-------
Nitrate H azteca
96 hour acute 72
hour
Estimated LC/EC Values and
Confidence
Limits
Exposure
95% Confidence Limits
Point
Cone.
Lower
Upper
LC/EC
1.00
14 .707
4 .730
23.770
LC/EC
5.00
25.899
12.466
36.406
LC/EC
10.00
35 .020
20.512
46.558
LC/EC
15 . 00
42 .929
28.212
55.930
LC/EC
50 . 00
101.522
76.466
172.319
LC/EC
85 . 00
240.086
149.195
737.500
LC/EC
90.00
294.310
173.098
1050.249
LC/EC
95.00
397.960
215.203
1777.611
LC/EC
99.00
700.818
322,226
4792.102
-------
Nitrate H azteca. 96 hour acute 72 hour
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10 +
9 +
8 +
7 +
6+
5 +
o . . . ,
5 +
.... o
4+ o , , ,
-o
3 +
2 +
1 +
O+o o
EC01 EC10 EC25 EC50 EC75 EC90 EC99
-------
¦TRIMMED SPEARMAN-KARBER METHOD. MONTANA STATE DNIV
FOR REFERENCE, CITE:
HAMILTON, M.A., R.C. RUSSO, AND R.V. THURSTON, 1977.
TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING MEDIAN
LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS.
ENVIRON. SCI. TECHNOL. 11(7): 714-719;
CORRECTION 12 (4):417 (1978).
DATE: 8/6/09 TEST NUMBER: 6112-12
CHEMICAL: NITRATE SPECIES: H AZTECA
RAW DATA:
CONCENTRATION(MG/L
NUMBER EXPOSED:
9.50 14.01 25.35 40.45 67.30 114.35
20 20 20 20 20 20
TRIM
50.00
DURATION (HOURS
72
48
LC50 LOWER 95% LIMIT UPPER 95% LIMIT PERCENT
114.35 prTrtyjjt tor ^ Kfifl.
98.28 83.78 115.29
35.00
10.00
96
-72.43
58.81
-------
Appendix 7: Raw Data for 96-hr Toxicity of
Nitrate on Pimephales promelas
-------
Raw Laboratory Data
-------
j-Li A_.
96-HOUR STATIC ACUTE TOXICITY TEST
TEST
MATERIAL:,
NO. ORGANISMS/CHAMBER:
PROJECT NUMBER: \ ^
TEST SPECIES: p-fofA ^ Q X
4A
PHOTQPERIOD (L:D):_
16:8
GLC#_
AGE/SOURCE OF ORGAN]
DILUTION WATER:
tRGANISM: . ,
: rj)J r\ J TEST TEMPERATURE ("Ch gJ^XI
. f0- \ LIGHT INTENSITY <10-20 uE/m'/s): Ambient DavO/ Day 4/
TEST
PAY
TEC Pi.
INITIALS
TREATMENT LEVEL
cJ&dkta
a s*y*L
(S?VC
OlcaoWl
1^K0H
B3C
TIME
REPUCATE NUMBER
i
2
1
2
i
4rf-—
Z
1
2
t
2
1
2
1
2
0
Teroperlrtiire f'CJ
;*>¦?
^sro
36*o
5SO
¦4
9&o
pH
?oj
frio
sw
K.C51
S.07
DO (ttmfU
K'M
W
^'4
ti<4
Sp. Cood. tumhesfcm)
no
l
©|?jo^f
1
m
Number Live
\ 0
\o
\o
\o
10
\o
vO
\0
\o
SO
8
£
Dt»«vatlofls
Temperature f'Cl
tK • if
3H' 2^
QH'^
gi.u
P«
7.11
1UB
7.^6
7.cs0
7,6?
7M1
DO (msin.)
e>,q
4.o
£>¦7
6*0
c>»0
s;i
SA
&,q
£>; W
2
<3-
Number Live
id
ID
10
/<>
i9
I i)
/•^
0
I
Observations
Temperature (*CJ
D43
24, 5
rati
1*15
nig-
TOonon.)
fo-fe
*>¦4
V!
/fc<3
0,1
S'-M'
r?(mgA.}
n.o
l.o
Q?(?
0&
Sp, CoexJ. [mwhosfcwij
3oa
qn*\
\H&
c2in^
SX O A
Observation Key; -
JU_ jo^O >0? 3o ,0 1-0 oo
.3, 1*. ^
EWEDETY: ^F^tV
DATE:
-------
Analytical Chemistry Data
-------
PROJECT#: 6112-12
Nitrate Nitrite Tox
CALIBRATION
CONCENTRATIONS
(ppm)
0.5
1
10
25
50
Date
Test Day
Natural
log
-0.6931472
0
2.30258509
3.21887582
3.91202301
Regression Output:
Constant
Std Err of Y Est
R Squared
No. of Observations
Degrees of Freedom
08/06/2009
0 tox test
Millivolts
-53.0
-62.9
-119.8
-141.2
-159.8
-66.194866
2.819348
0.997345
5
3
X Coefficient(s)
Std Err of Coef.
-23.537499
0.70114936
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
H. azteca
1 OOmg/L N03 |
0
water
3.5
-2.9610141
0.0517664
-0.0960736
H. azteca
lOmg/L N02
0
water
5.4
-3.0417363
rcr04775T9^
-0.1000881
r FHM
3000 mg/L N03
0
water
14.4
-3.4241049
0.03257843
-0.1152616
|| Blank
0
II -21.2
-1.9116248
0.14783999
0
-------
Nitrate Day 0 Fathead minnow Analytical Results 8,6.09
LabSamptelD
SampleDescription
ParamlD
Results Units
RL labQuaMer
AnalysjsMethod
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Parson
HB08060205
Dechlor Controi-nitrate
N03
1.119 mg/L
0.08854
A4500F
8/7/2009
8/6/2000 Ben COOk
8/7/2009
Ben Cook
HB06060205
Dectilor Control-nitrate
N02
<0.0036 mg/L
0.016425 U
A4500B
8/6/2009
8/6/2009 Jl
8/6/2009
Jl
HB08060206
3000mg/L-nitrate
N03
3338 mg/L
110.675
A4500F
8/7/2009
8/6/2009 Ben Cook
8/7/2009
Ben Cook
HB08060206A
3000mfl/L-nitrate
K02
<0.0036 mg/L
0.016425 U
A45008
8/6/2009
8/6/2008 Jl
8/6/2009
Jl
HB08060206B
aooomgyL-nitrate
N02
<0.0038 mgfl.
0.018425 U
A4500B
8/6/2009
8/6/2009 Jl
8/6/2009
Jl
HB08Q60207
1800mfl/L-nltrate
N03
1816 mg/L
110.675
A4500F
8/7/2009
8/6/2009 Ben Cook
8/7/2009
Ben Cook
HB0806020T
-iBQOmfl/L-nitrate
N02
0.0084 mg/L
0.016425 J
A4500B
8/6/2009
8/6/2009 Jl
8/6/200B
Jl
HB08060208
iQ80mg/L-nitrate
N03
1181 mg/L
44.27
A4500F
8/7/2009
8/6/2009 Ben Cook
8/7/2009
Ben Cook
HB08060208
lOSOmg/L-nitrate
N02
0.0084 mg/L
0.018425 J
A4500B
8/6/2009
8/6/2009 Jl
8/6/2009
Jl
HB080602C9
648mg/L-nitrate
N03
718 mg/L
22.135
A4500F
8/7/2009
8/8/2009 Ben Cook
8/7/2009
Ben Cook
HB08060209
648mg/L-nHrate
N02
<0.0038 mg/L
0,018425 U
A4500B
8/6/2009
8/6/2009 Jl
8/8/2009
Jl
HB08060210
389msj/L-nitrate
N03
432 mg/L
22.135
A4600F
8/7/2009
8/6/2009 Ben Cook
8/7/2009
Ben Cook
HB08060210
389mg/L-nitrate
N02
<0.0038 mg/L
0.018425 U
A4SOOB
8/6/2009
8/6/2009 Jl
8/6/2009
Jl
HB08060211
Stock 41.1g/L NaNO3-30g/L nitrate
N03
34000 mg/L
4427
A450OF
8/7/2009
8/6/2000 Ben Cook
8/7/2009
Ben Cook
HB0808Q211
Stock 41 ,lg/LNaNO3-30g/L nitrate N02
<0.0036 mg/L
0.016425 U
A4500B
8/6/2009
8/6/2009 Jl
B/S/2009
Jl
Page 1
-------
Nitrate Day 4 Fathead minnow Analytical Results 8,10.09
LabSamplelD
SampleDescription
PaiamlD
Results Units
RL LabQualifier
AnalyslsMethod
Anatysl»Date
Sample Date Analyst
Calibration Date
Calibration Person
HB08100240
Dechlof Control nitrate-Day 4
N02
0.0092
mg/L
0.018425 J
A4500B
8/12/2009
8/10/2009 Jl
8/12/2009
Jl
HB08100240A
Dechlor Control nitrate-Day 4
N03
1.089
mg/L
0.08854
A4500F
8/13/2009
8/10/2008 Ben Cook
8/13/2009
Ben Cook
HB08100240B
Dechlof Control nitrate-Day 4
N03
1.038
mg/L
0.08854
A4500F
8/13/2009
8/10/200S Ben Cook
8/13/2009
Ben Cook
HB08100241
3OO0mg/t,-nrtrate-Day 4
N03
3035
mg/L
110.875
A45Q0F
8/13/2009
8/10/2009 Ben Cook
8/13/2009
Ben Cook
HB08100241
3000mg/L-nitrate-Day 4
N02
0.469
mg/L
0.0057
A4500B
8/12/2009
8/10/2009 Jl
8/12/2009
Jl
HB08100242
1 8O0mg/L-nitrate-Day 4
N03
1782
mg/L
110.675
A4500F
8/13/2009
8/10/2009 Ben Cook
8/13/2009
Ben Cook
HB08100242
1 80Qmg/L-nitrate-Day 4
NOZ
0.360
mg/L
0.0657
A4500B
8/12/2009
8/10/2009 Jl
8/12/2009
Jl
HB08100243
lOBOmg/L-nitrate-Day 4
N03
1136
mjj/L
44.27
A4600F
8/13/2009
8/10/2009 Ben Code
8/13/2009
Ben Cook
HBOS100243
IQBOmg/L-nitrate-Day 4
N02
0.290
mg/L
0.0657
A4500B
8/12/2008
8/10/2009 Jl
8/12/2009
Jl
HB0810Q244A
S48mg/l-nitrate-Day 4
N03
892
mg/L
22.135
A4500F
8/13/2009
8/10/2009 Ben Cook
8/13/2009
Ben Cook
HB08100244A
848mg/L-nitrate-Day 4
N02
0.0716
mg/L
0,016425
A4500B
8/12/2009
8/10/2009 Jl
8/12/2009
Jl
HB0B100244B
648mg/L-nitrate-Day 4
N03
711
mg/L
22.135
A450QF
8/13/2009
8/10/2009 Ben Cook
8/13/2009
Ban Cook
HB08100244B
64Bmg/L-nitrate-Day 4
N02
0.0673
mg/L
0.016425
A4500B
8/12/2009
8/10/2009 Jl
8/12/2009
Jl
HBOB100245
389mg/L-nltrate-Day 4
N03
432
mg/L
22.135
A4500F
8/13/2009
8/10/2009 Ben Cook
8/13/2009
Ben Cook
HB0810024S
389mg/t-nitrate-Day 4
N02
0.0371
mg/L
0,016425
A4500B
8/12/2009
8/10/2009 Jl
8/12/2009
Jl
Page 1
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
G«<;«i Erv/ironrr.entaf Center
739 Hastings St
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
I „r 3
%(
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page of
Gwal L>iki:s Center
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax:231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
j . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number;
Collector:
^/b/oT
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below1
j*
BODs
Chloride
Chlorophyll a
Hardness
Z
tS
z
z
i
H
1
©7
Z'
TDS
. • '
- N
h
H
cn •
: ¦ " "•;!
Nitrate
Nitrite
FW-00-09
8/6/09
125 mL
HOPE
389 mg/L - nitrate
h2so4
-'.V . -i
r T: ': ~
FW-00-10
8/6/09
125 mL
HDPE
3000 mg/L - nitrate
Cold
-1
"
- -
X
FW-00-11
8/6/09
125 mL
HDPE
1800 mg/L - nitrate
Cold
-
ilSft
'•
X
FW-00-12
8/6/09
125 mL
HDPE
1080 mg/L - nitrate
Cold
X
FW-00-13
8/6/09
125 mL
HDPE
648 mg/L - nitrate
Cold
r,y.yr%
-
Tv?7 -
X
FW-00-14
8/6/09
125 mL
HDPE
389 mg/L - nitrate
Cold
; ...^
X
Shipper:
'Ur^ilu
2.
Date/Time:
Receiver:
Date/Time:
Temperature:
BODj = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids ' TP = Total Phosphorus
NHj-N ™ Ammonia-Nitrogen TKN" Total Kjcldahl Nitrogen TSS - Total Suspended Solids
NOj/N02-N = Nitrate/Nitrite-Nitrogen
Alibrcvia Hons for other frequently requested analyses
N0i-N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N -Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page
3 »r3_
CiKf-VI Lakes Envircnmrniiul t
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
m, garton
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below'
Bottle ID
Alkalinity
Q
O
83
Chloride
| Chlorophyll a
Hardness
at
¦
S
z
T.
1
, O
Z
O
z
en
a
H
i
&
H
•''-uiA
¦ -m.:-
1
Nitrate
J Nitrite
FW-00-03
8/6/09
125 mL
HDPE
Stock 41.1 g/L
NaN03 -
30 g/L nitrate
h2so4
j f
X
FW-00-04
8/6/09
125 mL
HOPE
Stock'41.1 g/L
NaN03-
30 g/L nitrate
Cold
**• if
".
X
-
¦ •
v. v,
I .
ftlll
Shipper:
Date/Time:
Receiver:
^ 9J
Date/Time:
Temperature:
BODj ¦= Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NO3/NO1 -N = Nitrate/Nitrite-Nitrogen
iDeBititioiis ' -' ;v'"
TOS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NO; -N --NitriteNitrogen Total N = Total Nitrogen
NO) -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
j f /——I
vjLlL-
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
wvvvv.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page j of
vzw 'Eliolw
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site Information /
sample type)
Preservation
Check the requested analysis (see definitions below)
¦
''"iS-"-.:-:
1 '¦
¦- ;2gr
«n
Q
O
oa
¦"u
¦;2' -
-2. .•
u ¦.
| Chlorophyll a
Hardness
T.
£
z
¦•5E-
1-
o
- W
o
S5
TDS
• "Sc-v"-
.. . f!T. '
3.
H
m ¦
' '4»:
. •'
»¦
S5.
Nitrite
FW-04-01
8/10/09
125 mL
HDPE
Dechlor Control -
nitrate-Day 4
h2so4
FW-04-02
8/10/09
125 mL
HDPE
Dechlor Control -
nitrate- Day 4
Cold
X
FW-04-03
8/10/09
125 mL
HDPE
3000 mg/L - nitrate-
Day 4
h2so4
X '¦'
FW-04-04
8/10/09
125 mL
HDPE
1800 mg/L - nitrate-
Day 4
h2so4
W
fW-04-05
8/10/09
125 mL
HDPE
1080 mg/L - nitrate-
Day 4
h2so4
SF"
fW-04-06
8/10/09
125 mL
HDPE
648 mg/L - nitrate-
Day 4
h2so,
€
Shipper: Date/Time:
Receiver:
Date/Time:
Temperature:
BOD? = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NOi/N0i-N.7 Nitrate/Nitrite-Njtrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS =;¦ Total Suspended Solids
Abbreviations for otherTreaueatlv requested analyses
NO? -N = Nitrite Nitrogen Total N = Total Nitrogen
i, _N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
f NO
L
-------
CHAIN OF CUSTODY RECORD
{To be completed and submitted with samples)
wsm-
Page
arton/ramie
G-XJUt LilKt'-i I C;:iV:.-'
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.com
Facility;
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person- bailee GartonAfamie Saxton
Email Address:
Phone Number:
Collector:
m. carton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below"
1
<
O
S
Chloride
1 - 1
Chlorophyll a
t
&
z
X
z
1;
w
a
H
p"
cu
H
X
FW-04-08
8/10/09
125 mL
HOPE
3000 mg/L - nitrate-
Day 4
Cold
: . ;•
X
FW-04-09
8/Jbf/09
P
125 mL
HDPE
1800 mg/L - nitrate-
Day 4
Cold
- > "
X
FW-04-10
8/10/09
125 mL
HDPE
1080 mg/L - nitrate-
Day 4
Cold
: \ "V - '•
X
FW-04-11
8/10/09
125 mL
HDPE
648 mg/L - nitrate-
Day 4
Cold
X
FW-04-12
8/10/09
125 mL
HDPE
389 mg/L - nitrate-
Day 4
Cold
¦
X
Shipper:
Date/Time:
¦ft] tojrf, pspq
Receiver:
cJ
Date/Time:
^ I '0
Temperature:
Definitions
BOD. = Biochemical Oxygen Demand-5 day TDS - Total Dissolve Solids TP = Total Phosphorus
NHi -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NO/N02 -N = Nitrate/Nitrite-Nitrogen
Abbreviations for--other frequently requested i
N02 -N - Nitrite Nitrogen Total N = Total Nitrogen
NOj -N =Nitrate Nitrogen SRP Soluble Reactive Phosphorus
-------
Reference Toxicant Data
-------
Fathead Minnow Survival
Sodium Chloride (NaCl) Toxicity Data
2007-2009
^ (\b t\^ c?1 c? c?
n# n# r# n# ^ r# r# n# ^ ^ r# ,# r#
>*¦ v \v jy ~ k j-jv ~*y jv x* \ * r* ~v ^-jv <> Arv
4> & .$¦ $ ^ £> # o° # <$> $> ^ <$¦ ^ £>
Wr 48 hour LC50 ~£r- Mean plus 2 S.D. —I— Mean minus 2 S.D.
-------
Statistical Analyses
-------
SPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
Nitrate FHM 96 hour acute 4 8 hour
Proportion
Observed
Responding
Predicted
Number
Number
Proportion
Adjusted for
Proportion
Cone.
Exposed
Resp.
Responding
Controls
Responding
432.0000
20
0
0.0000
0.0000
0.0027
707.0000
20
0
0.0000
0.0000
0.0220
1158.,0000
20
4
0.2000
0.2000
0.1068
1799.0000
20
4
0.2000
0.2000
0.2890
3186.5000
20
13
0.6500
0.6500
0.6314
Chi - Square for Heterogeneity (calculated)
Chi - Square for Heterogeneity
(tabular value at 0.05 level)
3.127
7.815
Mu
Sigma
3.409899
0.278322
Parameter
Intercept
Slope
Estimate
-7.251626
3.592959
Std. Err.
2.563425
0.783487
95% Confidence Limits
-12.275939,
2.057323,
-2.227312)
5.128594)
Theoretical Spontaneous Response Rate = 0.0000
-------
Nitrate FHM 96 hour acute 48 hour
Estimated LC/EC Values and Confidence Limits
Exposure 95% Confidence Limits
Point Cone. Lower tipper
LC/EC
1
.00
578.679
233.316
856.
131
LC/EC
5
.00
895.544
488.349
1190.
760
LC/EC
10
.00
1130.319
715.135
1437.
455
LC/EC
15
00
1322.652
915.229
1649.
856
LC/EC
50
00
2569.797
2059.163
3725
030
LC/EC
85
00
4992.888
3513.939
11088
438
LC/EC
90
00
5842.471
3948.665
14494
680
LC/EC
95
00
7374.131
4681.912
21610
334
LC/EC
99
.00
11411.945
6414.052
45922
340
-------
Nitrate FHM 96 hour acute 48 hour
PLOT OF ADJUSTED FROBITS AND PREDICTED REGRESSION LINE
Probit
10+
9+
8+
1 +
6+ ....
- . o» .
5+
o .... o
4 +
3+
2 +
1+
O+o o
EC01
EC10 EC25 EC50 EC75 EC90
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
Nitrate FHM 96 hour acute 72hour
Cone.
Number
Exposed
Number
Resp.
Observed
Proportion
Responding
Proportion
Responding
Adjusted for
Controls
Predicted
Proportion
Responding
432
0000
20
0
0
0000
0
0000
0.0023
707
0000
20
0
0
0000
0
0000
0.0218
1158
0000
20
4
0
2000
0
2000
0.1159
1799
0000
20
5
0
2500
0
2500
0.3223
3186
5000
20
14
0
7000
0
7000
0.6886
Chi - Square for Heterogeneity (calculated)
Chi - Square for Heterogeneity
(tabular value at 0.05 level)
2.361
7.815
Mu
S x gma
3.375194
0.260499
Parameter
Intercept
Slope
Estimate
-7.956661
3.838790
Std. Err.
2.599883
0.795797
95% Confidence Limits
-13.052431,
2.279027,
-2.860891)
5.398553)
Theoretical Spontaneous Response Rate = 0.0000
-------
Nitrate FHM 96 hour acute 72hour
Estimated LC/EC Values and Confidence Limits
Exposure
95% Confidence Limits
Point
Cone.
Lower
Dpper
LC/EC
1
00
587.754
259.892
850.253
LC/EC
5
00
884.503
506.564
1161.213
LC/EC
10
00
1099.863
715.872
1384.896
LC/EC
15
00
1274.127
896.644
1572.685
LC/EC
50
00
2372.431
1935.397
3230.165
LC/EC
85
00
4417.479
3241.098
8551.365
LC/EC
90
00
5117.393
3623.333
10879.996
LC/EC
95
.00
6363.379
4262.685
15586.874
LC/EC
99
.00
9576.171
5752.918
30744.047
-------
Nitrate FHM 96 hour acute 72hour
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10+
9+
8+
7 +
6+
5+
4+
o
3+
2 +
c
1+
G+o o
— H: j -f. | _ ^
EC01 EC10 EC25 EC50 EC75 EC90 EC
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
Nitrate FHM 96 acute
Proportion
Observed
Responding
Predicted
Number
Number
Proportion
Adjusted for
Proportion
Cone.
Exposed
Resp.
Responding
Controls
Responding
432.0000
20
0
0.0000
0.0000
0.0079
707.0000
20
1
0.0500
0.0500
0.0559
1158.0000
20
5
0.2500
0,2500
0.2212
1799.0000
20
10
0.5000
0 .5000
0.4864
3186.5000
20
16
o.aooo
0 .8000
0.8208
Chi - Square for Heterogeneity (calculated.)
Chi - Square Cor Heterogeneity
(tabular value at 0.05 level)
0.343
7 . 815
Mu
Sigma
3 .263946
0 .260637
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept -7.522968 2.231594 ( -11.896892, -3,149044)
Slope 3.83S757 0.696896 ( 2.470841, 5.202672)
Theoretical Spontaneous Response Rate = 0.0000
-------
Nitrate PHM 96 acute
Estimated LC/EC Values and Confidence Limits
Exposure 95% Confidence Limits
Point Cone. Lower Upper
LC/EC
1
00
454
597
220
828
653
984
LC/EC
5
00
684
266
409
626
899
460
LC/EC
10
00
850
970
565
482
1073
533
LC/EC
15
00
985
876
699
188
1216
204
LC/EC
50
00
1836
311
1518
008
2328
008
LC/EC
85
00
3420
348
2624
170
5596
595
LC/EC
90
00
3 962
583
2948
999
6976
031
LC/EC
95
00
4927
966
3494
085
9700
944
LC/EC
99
00
7417
640
4773
737
18114
947
-------
Nitrate FHM 96 acute
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10+
9 +
7+
6 +
. . .o
5+
4 +
3 +
2 +
1 +
O+o
- -j — — - - ~
EC01 EC10 SC25 EC50 EC75 EC90 EC99
-------
Appendix 8: Raw Data for 32-day Toxicity of
Nitrate on Pimephales promelas
-------
Raw Laboratory Data
-------
GlbC
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
TEST MATERIAL: TYPE OF TEST: V\.0\ jQ Tva hMfclLUTION WATER:
PROJECT NUMBER: If)I IP' [> NUMBER OF FISH/CHAMBER: '^O J> DILUTOR NUMBER: PHOTOPERIOD: lU' ^
TEST SPECIES: NUMBER OF CHAMBERS: ^ TEST TEMPERATURE: <35"t (* C- AGE OF FISH:
DATE
TEST
TIME
(DAYS)
TECH.
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PM - Particulate Matter
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ERR - Erratic Swirr
FS - Film on Surface
F - Floater
[MM - Immobile
-------
OTFr
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
Gtest Lakaa Environ rnental Center
TEST MATERIAL:
PROJECT NUMBER:_
TEST SPECIES:
TYPE OF TEST
NUMBER OF FfSH/CMAMBl
NUMBER OF CHAMBERS;
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£ DILUTION WATER;
DILUTOR NUMBER:
Mm.
PHOTOPERiOO:
TEST TEMPERATURE: _ AGE OF Embryos:_
DATE
TEST
TIME
(DAYS)
TECH,
INIT,
TREATMENT
250^ iL^
TIME
REPLICATE NUMBER
1
2
3
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Observation Key: DOB - Dried Out on Chamber PM • Particulate Matter ERR - Erratic Swimming FS - Film on Surface F - Floater IMM - Immobile FU - Fungus
Dated: Reviewed l
-------
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
Etwfrortmcjiial Center
TEST MATER IAL;_ ^ A TYPE OF TEST: DILUTION WATER: Q*cb\OC
PROJECT NUMBER: (1 %' NUMBER OF FISH/CHAMBER: SO fl j 7-fc V*M' DILUTOR NUMBER: fi-td PHOTOPERIOP: i & 1 B
TEST SPECIES: QU4 NUMBER OF CHAMBERS: TEST TEMPERATURE: 3*5 * j °l . AGE OF Embryos:
Observation Key: DOB - Dried Out on Chamber PM - Particulate Matter ERR - Erratic Swimming. FS - Film on Surface F - Floater IMM - Immobile FU • Fungus
Lj-J
Dated: Reviewed b 1
-------
GleC
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
Great takes EnvlrosmeftUtf Center
TEST MATERIAL:
K\vV(tu3aP
PROJECT NUMBER:_
TEST SPECIES:_
MP-
TYPE OF TEST
NUMBER OF FISWCHAMBER:,
NUMBER OF CHAMBERS:
Dj.4,j/kA&*< "P1o»aj
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DILUTION WATER:
DILUTOH NUMBER:
PHOTOPERIOD:
TEST TEMPERATURE: IPSS t"^ AGE OF Embryos:.
DATE
TEST
TIME
(DAYS)
TECH.
INIT.
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VP
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Dated:
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Reviewed by: (jn^eJLu
-------
GleC
FATHEAD MINNOW EARLY UFE-STAGE FLOW-THROUGH TOXICITY TEST
<3reat Lafa&s gfprtrorjrrseotsi Ceme?
TEST MATERIAL:
PROJECT NUMBER:
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(DAYS)
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Dated: Reviewed i
J xJ^uJU* 6-3
-------
Great Lakes Envfe-onrrjeninl Center
TEST MATERIAL: N;-hro-R,
PROJECT NUMBER:_
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FATHEAD MINNOW EARLY Li -STAGE FLOW-THROUGH TpXICITY TEST
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(DAYS)
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F - Floater
IMM - Immobile
FU - Fungus
-------
GtfC
Great Latea fiuvfronmeniat Center
TEST MATERIAL:
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
Ni'Wa
TYPE OF TEST:
PROJECT NUMBER:_
TEST SPECIES:
NUMBER OF F1SH/CH,
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(DAYS)
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PM - Particulate Matter
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F - Floater
IMM - Immobile FU - Fungus
-------
m pr
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
©reat Lakes EswfronmooKit Center
TEST MATERIAL:
TYPE OF TEST: i J ik /IM. a\L DILUTION WATER:
PROJECT NUMBER: Op|,Q* I > NUMBER OF FISH/CHAMBER: S ^ 1A ^3^ OiLUTOR NUMBER: $-XjA PHOTOPERIOO: j U - Y
TEST SPECIES:
NUMBER OF CHAMBERS: [J " TEST TEMPERATURE: C AGE OF Embryos:
Observation Key; DOB - Dried Out on Chamber PM * Particulate Matter ERR ^Erratic Swimming FS - Film on Surface F - Floater 1MM - Immobile FU - Fungus
Dated: ' Reviewed by
ter ERR • Erra
-------
GleC
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
Lates E-ivfremrnems! Centef
TEST MATERIAL:
PROJECT NUMBER:.
TEST SPECIES:
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NUMBER OF FISWCHAMBER
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DILUTOR NUMBER:
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DATE
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IMM - Immobile FU - Fungus
-------
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
Gfea.? Lakes Ef^&onmcntai Censer
TEST MATERIAL:
TYPE OF TEST
PROJECT NUMBER:_
TEST SPECIES:
NUMBER OF FtSH/CHA
NUMBER OF CHAMBERS:
DILUTION WATER:_
DILUTOR NUMBER:
PHOTOPERIOD:
U'.V
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DATE
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TIME
(DAYS)
TECH.
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Observation Key:
Dated:
DOB - Dried Out on Chamber
PM - Particulate Matter
Reviewed
ERR - Erratic Swirrimi
FS - Film on Surface
F - Floater
IMM - Immobile
FU - Fungus
-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrate
Dilution Water Contro
Fish Number
Rep 1
Rep 2
Rep 3
Rep 4
1
0.10011
0.07759
0.10816
0.00190
2
0,02507
0.08725
0.08901
0.00169
3
0.01763
0.05185
0.01422
0.12338
4
0.02727
0,05362
0,10388
0,05778
5
0.02375
0.05222
0.04789
0.04874
6
0.06915
0.03647
0,03026
0,05424
7
0.02629
0.04315
0.02148
0.04286
8
0.02010
0,03909
0.01000
0.03437
9
0,02708
0.03305
0.01485
0.02000
10
0.01239
0.01812
0.00876
0.03057
11
0.04217
0.01439
0.00399
0,01695
12
0.01670
0,00889
0.00654
0.01011
13
0.01764
0.00889
0.00747
0.00069
14
0.01844
0.00400
0.00800
0.04341
15
0.00424
0.00313
0.00646
0,05610
16
0.00427
0.00130
0.00427
17
0,00239
0.00100
0,00607
18
0.00240
0.05420
0.04796
19
0.06024
20
0,00051
N
18
18
20
15
Min
0.00239
0.00100
0.00052
0,00069
Max
0.10110
0.08725
0.10816
0,12338
Mean
0,02545
0.03268
0.03000
0.03619
-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrate
125 mg/
L Nitrate
Fish Number
Rep 1
Rep 2
Rep 3
Rep 4
1
0.00858
0.01952
0.04684
0,01677
2
0.00352
0.00820
0.06732
0.01150
3
0.00439
0.07721
0.01858
0.06247-
4
0.05775
0.06570
0.03579
0,06734
5
0.07432
0.04137
0.01427.
0.03508
6
0.03083
0.03416
0.05155,
0.03492
7
0.0224?
0.03992,
0.04357.
0.01860
8
0.06628
0.07594
0,03693
0.01554
9
0.04569
0.05350.
0.03544
0.02006
10
0.08368
0.03573
0.03519
0.06195
11
0.00930'
0.03252
0.01002
0.04085
12
0.04249
0.03035
0.02795
0.03150
13
0,01737
0.01974
0.02605,
0.02602
14
0.03109
0.00352
0.02526
0.03531
15
0.02687
0.00159
0.01450
0.02772
16
0.01764
0.01760
0.01000
17
0.01807
0.03230
0.00694
18
0.09415
0.00161
0.00056
19
20
N
18
15
18
18
Min
0.00352
0.00159
0.00161
0.00056
Max
0.09415
0.07721
0.06732
0.06734
Mean
0.03636
0.03593
0.03004
0.02906
-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrate
250 mg/
L Nitrate
Fish Number
Rep 1
Rep 2
Rep 3
Rep 4
1
0.01287
0.04991
0.00743
0.06063
2
0.01219
0.01830
0.04281
0.00493
3
0.03748
0.02890
0.04263
0.05600
4
0.03280
0.07032
0.02909
0.03827
5
0.06677
0.03353
0.01365
0.02362
6
0.01467
0.04522
0.04856
0.09027
7
0.03487
0.01807
0.03406
0.07485
8
0.00534
0.03424
0.03184
0.02531
9
0.04021
0.01020
0,02274
0,01187
10
0.03910
0.01230
0.02670
0.03999
11
0.00396
0.01283
0.03754
0.02643
12
0.07570
0.03733
0.03202
0.02845
13
0.01164
0.02182
0.03495
0.03622
14
0.02357
0.03421
0.02559
0.00829
15
0.01950
0.04731
0.01378
0.01025
16
0.02315
0.03560
0.00051
17
0.00976
0.03878
0.00411
18
0.02899
0.02665
0.00653
19
0.07411
0.03480
0.01362
20
0.03431
0.06757
N
19
20
20
15
Min
0.00396
0.01020
0.00051
0.00493
Max
0.07570
0.07032
0.06757
0.09027
Mean
0.02983
0.03223
0.02679
0.03569
-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrate
500 mg/
L Nitrate
Fish Number
Rep 1
Rep 2
Rep 3
Rep 4
1
0.07401
0.07820
0.06320
0.07064
2
0.08320
0.05008
0.03828
0.04439
3
0.01487
0.03431
0.08520
4
0.01981
0.08480
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
N
3
4
4
2
Min
0.01487
0.01981
0.03828
0.04439
Max
0,08320
0.07820
0.08520
0.07064
Mean
0.05736
0.04560
0.06787
0.05752
/
-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrate
1000 mg/L Nitrate
Fish Number
Rep 1
Rep 2
Rep 3
Rep 4
1
0.02052
0.05815
0.04881
0.01139
2
0.07429
0.05750
3
0.05540
4
0.02162
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
N
1
4
2
1
Min
0.02052
0.02162
0.04881
0.01139
Max
0.02052
0.07429
0.05750
0.01139
Mean
0.02052
0.05237
0.05316
0.01139
J
-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrate
2000 mg/L Nitrate
Fish Number Rep 1 Rep 2 Rep 3 Rep 4
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
N
Min
Max
Mean
-------
- 28
BOOK PAGE
TITLE
PROJECT
10
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BOOK
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-------
32-Day Early Life-StageToxicity Test Fish Weight Data for
HECD B-12 Nitrate
Pimephales prometas (Fathead Minnow)
A
B
A-B
C
A-B/C
A/20
Weight of
Weight of
Average Dry
Biomass
Nominal (and Measured)
Pan and Fry
Oven-Dried
Total Dry
Number of
Fry Weight
Weight
Test Concentration
(g)
Pan
Weight (g)
Fry Weight
(mg)
(mg)
rep 1
0.91568
0.80895
0.10673
18
5.929
5.34
rep2
0.93023
0.81138
0.11885
18
6.603
5.94
Dilution
rep3
0.91429
0.80120
0.11309
20
5.655
5.65
water/Control
rep4
0,91781
0.82200
0,09581
15
6.387
4.79
rep 1
0.93891
0.80650
0.13241
18
7.356
6.62
rep2
0.92098
0.80749
0.11349
15
7.566
5.67
rep3
0.91670
0.81000
0.10670
18
5.928
5.34
125 (95.9) mg/L
rep4
0.91587
0.81074
0.10513
18
5.841
5,26
rep 1
0.91710
0.80810
0.10900
19
5.737
5.45
rep2
0.93258
0.81302
0.11956
20
5.978
5,98
rep3
0.90974
0.80466
0.10508
20
5.254
5.25
rep4
0.91724
0.80797
0.10927
15
7.285
5,46
250 (215) mg/L
rep 1
0.84168
0.80611
0.03557
3
11.857
1.78
rep2
0.84416
0.80738
0.03678
4
9.195
1.84
rep3
0.86653
0.81080
0.05573
4
13.933
2.79
500 (483) mg/L
rep4
0.83886
0.81380
0.02506
2
12.530
1.25
rep 1
0.80579
0.80184
0.00395
1
3.950
0,20
rep2
0.84939
0.80895
0.04044
4
10.110
2.02
rep3
0.82900
0.80822
0.02078
2
10.390
1.04
1000 (999) me/L
rep4
0.80354
0.80159
0.00195
1
1.950
0.10
-------
GleC
CHRONIC TOXICITY TEST - WEIGHT DATA
Great'Latofi Envi-onmentalCenter
TEST MATERIAL;
TEST NUMBER;
TECHNITIAN'S INITIALS: fuJy
QC INITIALS;
WEIGHT DATE: TYPE/MODEL OF DRYING OVEN:
"""""" " * ^' % 1 Silpd^l OVEN TEMPERATURE (°C):
[ON ffaoljRS):' r24 TESTS
TEST DATE:
DRYING DURATION (
Blue M
60
' SPECIES:.
FHM
Treatment
Level
Rep.
A |
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fe)
B
Weight of
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Pans (g)
A-B
Total Dry
Fry Weight
GO
c
Number of
Fry Weight
A-B/C
Average Dry
Fry Weight
(mg)
Biomass
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-------
Analytical Chemistry Data
-------
Measured Data for the 32-Day Toxicity Test ^
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
Nominal Nitrate Test Concentration Dilution Water/Control
Sample
Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
no3
no2
ng3
no2
no3
no2
no3
no2
07/22/09
-2
1.009
0.0338
1,081
0.0237
1.004
0.0243
1.009
0.0161
0.984
0.0174
07/23/09
-1
0,974
0.0276
0.9831
0.0283
1.001
0.0348
0,9261
0.0223
0.987
07/24/09
0
1.046
0.0141
1,103
0.0269
1.104
0.0141
1.062
0.0122
1.118
0.0122
07/27/09
3
1.01
0.0105
07/29/09
5
1.36
<0.0036
07/31/09
7
1.276
0,0046
•1.272
08/03/09
10
1,198
0,0072
1,168
08/05/09
12
1.179
<0.0036
<0.0036
08/07/09
14
1.109
0.0055
08/10/09
17
1.049
0.0061
08/12/09
19
1.087
0,0113
08/14/09
21
1.137
0.0125
0.0145
08/17/09
24
1.197
0.0089
1,156
08/19/09
26
1.098
<0.0036
1.151
08/21/09
28
1.131
<0.0036
08/25/09
32
<0.021
0.0072
1.106
0.0049
1.047
0.0067
1.05
0.0135
<0.021
1.0602 0,0127
0.9261 <0.0036
1.1980 0.0223
Mean of all NOs 1.0269
Min of all N03 <0.021
Max of all N03 1.3600
Mean 0.7426 0.0172 1,1262 0,0133 1.1258 0.0113
Min <0,021 ' 0.0072 0.9831 <0.0036 1.0010 <0,0036
Max 1.1370 0.0338 1.3600 0.0283 1.2760 0.0348
Mean of all N02
Min of all N02
Max of all NOz
0,0134
<0.0036
0,0139
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
Nominal Nitrate Test Concentration 125.0 mg/L
Sample
Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
no3
no2
no3
no2
no3
no2
no3
no2
07/22/09
-2
75.4
0.0214
65.8
0.0263
73.1
0.021
65.7
0,0348
07/23/09
-1
69.2
0.0296
72.1
0.026
66.7
0.026
69
0.0256
70.4
0.0283
07/24/09
0
54.1
0.0174
55.8
0.0115
62.3
0.0164
58.8
0.0082
07/27/09
3
74.3
0.0427
07/29/09
5
07/31/09
7
105.7
0.0069
08/03/09
10
08/05/09
12
95.9
<0.0036
08/07/09
14
08/10/09
17
78.5
0.0099
08/12/09
19
08/14/09
21
81.5
0,012
08/17/09
24
08/19/09
26
105.8
0,0101
08/21/09
28
08/25/09
32
178
0.0076
181.4
<0.0036
181.2
<0,0036
181
0.0131
I
180.7
0.0095
Mean
Min
Max
Mean of all N03
Min of all N03
Max of all N03
88.7500 0.0218
54.1000 0,0076
178,0000 0.0427
95.9333
54.1000
181.4000
97,7667 0.0138
55,8000 <0.0036
181.4000 0.0263
91.6000 0.0159
62.3000 <0.0036
181.2000 0.0283
105,6167 0.0169
58,8000 0.0082
181.0000 0.0348
Mean of all N02
Min of all NG2
Max of all N02
0.0171
<0.0036
0.0427
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
Nominal Nitrate Test Concentration 250.0 rog/L
Sample
Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
no3
no2
no3
no2
no3
no2
no3
no2
07/22/09
-2
169,6
0.022
173.2
0.021
168.8
0.022
173.4
0,023
07/23/09
-1
200
0.0243
180.1
0.0269
232.9
0,0315
190.1
0.0283
07/24/09
0
180.7
0.0122
179
0.0164
177,7
0.0069
184.6
0.0112
178.3
0.0141
07/27/09
3
07/29/09
5
232
<0,0036
07/31/09
7
08/03/09
10
162
0,0079
08/05/09
12
08/07/09
14
245.9
0.0066
08/10/09
17
08/12/09
19
187.9
0.0314
08/14/09
21
08/17/09
24
252
0.125
08/19/09
26
08/21/09
28
273.6
0.0186
08/2b/09
32
285.6
0.0122
295,9
0.0154
303.7
0.0122
309.5
<0.0036
Mean
Min
Max
Mean of all NOj
Min of all NOa
Max of all N03
214.0286 0.0302
169.6000 <0,0036
285.6000 0.1250
214.6304
162.0000
309.5000
210.6333' 0.0177
162.0000- 0.0079
295.9000 0.0269
225.8000 0.0158
168.8000 0.0066
303.7000 0.0315
209.1000 0.0191
173.4000 <0.0036
309.5000 0.0314
Mean of all N02
Min of all N02
Max of all NOa
0.0214
<0.0036
0.1250
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promeias (Fathead Minnow)
Nominal Nitrate Test Concentration 500.0 mg/L
Sample
Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
no3
no2
no3
no2
no3
NOz
no3
no2
07/22/09
-2
425
0.0273
443
0.0171
391
0.0207
409
0,0187
421
0.0194
07/23/09
-1
455
0.0302
472
0.0237
470
0.0273
522
0.0276
0.0933
07/24/09
0
443
0.0154
448
0.0145
457
0.0207
450
0.0099
07/27/09
3
505
0.0108
452
0.0102
07/29/09
5
07/31/09
7
476
0,0141
08/03/09
10
08/05/09
12
443
<0.0036
<0.0036
08/07/09
14
08/10/09
17
424
0.0135
08/12/09
19
08/14/09
21
398
0.01
08/17/09
24
08/19/09
26
599
0.0109
0.0092
08/21/09
28
08/25/09
32
645
0.0177
626
<0.0036
605
0.0095
615
0,0427
Mean
Min
Max
Mean of all N03
Min of ail N03
Max of all N03
474,7143 0.0269
398.0000 0.0100
645,0000 0.0933
483.0833
391.0000
645.0000
497.8571 0.0138
421.0000 <0,0036
626,0000 0.0237
473.2000 0.0136
391.0000 <0.0036
605.0000 0.0273
484.0000 0.0225
409.0000 0.0099
615.0000 0.0427
Mean of afl N02
Min of all NOz
Max of all NO*
0.0193
<0.0036
0.0933
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promeias {Fathead Minnow)
Nominal Nitrate Test Concentration 1000.0 mg/L
Sample
Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
¦ no3
no2
NOj
no2
no3
no2
no3
no2
07/22/09
-2
828
0.0273
744
0.0223
760
0.0289
700
0.022
07/23/09
-1
697
0.0302
930
0.0361
790
0.0238
820
0.0263
780
0.0273
07/24/09
0
967
0.0125
951
0.02
939
0.0171
959
0.0122
942
0,0135
07/27/09
3
07/29/09
5
1098
<0.0036
07/31/09
7
08/03/09
10
1027
0.0463
08/05/09
12
08/07/09
14
1222
0.0155
08/10/09
17
08/12/09
19
1218
0.0216
08/14/09
21
08/17/09
24
1190
0,0161
08/19/09
26
08/21/09
28
1191
1.33
1.35
08/25/09
32
1262
0.014
1327
2.4
1315
0.0218
1316
0.0158
Mean
Min
Max
974.5714 0.0185
697.0000 <0.0036
1262.0000 0,0302
1028.3333
744.0000
1327.0000
0.7435
0.0200
2.4000
994.6667
760.0000
1315.0000
0.0201
0.0135
0.0289
1002.6000
700.0000
1316.0000
0.0196
0.0122
0,0263
Mean of all no3
Min of all N03
Max of all NOj
998.8750
697,0000
1222.0000
Mean of all N02
Min of all NOj
Max of all N02
0.2221
<0.0036
2.4000
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
Nominal Nitrate Test Concentration 2000.0 mg/L
Sample
Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
no3
no2
no3
no2
NOs
no2
no3
no2
07/22/09
-2
1720
0.0243
1510
0.0283
1990
0.0391
1950
0.0289
1970
0.0411
07/23/09
-1
2020
0.0302
1640
0.026
1820
0.0384
1820
0.0378
0.0338
07/24/09
0
2037
0.0072
2017
0.0213
2041
0.0266
2051
0.0131
07/27/09
3
2149
0.0154
07/29/09
5
07/31/09
7
2056
<0.0036
08/03/09
10
08/05/09
12
2183
0.383
08/07/09
14
08/10/09
17
1922
0.0205
08/12/09
19
08/14/09
21
2101
0.017
08/17/09
24
08/19/09
26
2087
0,0437
08/21/09
28
08/25/09
32
Mean 2005.4000 0.0188 1862.0000 0.0242 2000.8000 0.1056 1935.7500 0.0268
Win 1720.0000 0.0072 1510.0000 <0.0036 1820.0000 0.0266 1820.0000 0.0131
Max 2149.0000 0.0302 2087.0000 0.0437 2183.0000 0.3830 2051.0000 0.0378
Mean of all NO, 1951,7895
Min of all N03 1510.0000
Max of all N03 2183.0000
Mean of all N02 0,0439
Min of all N02 <0.0036
Max of all N02 0.3830
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
Nominal Nitrate Test Concentration Stock Solutions
Sample
Date
Test Day
Stock Solution 59.4
g/L
Stock Solution
237.6 g/L
no3
no2
no3
no2
07/22/09
-2
49400
0.0069
181200
0,0194
0.0194
07/23/09
-1
07/24/09
0
07/27/09
3
07/29/09
5
41900
<0.0036
07/31/09
7
08/03/09
10
08/05/09
12
08/07/09
14
46300
<0.0036
08/10/09
17
08/12/09
19
08/14/09
21
45000
0,0046
08/17/09
24
46100
0.0039
08/19/09
26
08/21/09
28
08/25/09
32
46100
0.0049
0.0049
Mean
Min
Max
45800.0000 0.0048
41900.0000 <0.0036
49400.0000 0.0069
-------
Nitrate Day-2 FHM 32 Day Analytical Results 7.22,09
LabSamplelD
SampleDescription
ParamID
Results
Units
RL LabQualifier
AnalysisMethod
AnalysisDate
SampleDate
Analyst
Calibration Dale
Calibration Person
HB07220001
Water Control Tank #1
N02
0.0338
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
, HB07220001
Water Control Tank #1
N03
1.009
mg/L
0.08854
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
f HB07220002
Water Control Tank #2
N02
0.0237
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
/ HB07220002
Water Control Tank #2
N03
1.081
mg/L
0.08854
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
sj HB07220003
Water Control Tank #3
N02
0.0243
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07220003
Water Control Tank #3
N03
1.004
mg/L
0.08854
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
HB07220004A
Water Control Tank #4
N02
0.0161
mg/L
0.016425 J
A45008
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07220004A
Water Control Tank #4
N03
1.009
mg/L
0.08854
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
HB07220004B
Water Control Tank #4
N02
0.0174
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07220004B
Water Control Tank #4
N03
0.984
mg/L
0.08854
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
HB07220005
Water 125mg/L Tank #1
N02
0.0214
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07220005
Water 125mg/LTank #1
N03
75.4
mg/L
4.427
A450QF
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
/ HB07220006
Water 125mg/L Tank #2
N02
0.0263
mg/L
0.016425
A450QB
7/23/2009
7/22/2009
JB
7/23/2009
JB
Sj HB07220006
Water 125mg/L Tank #2
N03
65.8
mg/L
4.427
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
HB07220007
Water 125mg/L Tank #3
N02
0.0210
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07220007
Water 125mg/L Tank #3
N03
73.1
mg/L
4.427
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
HB0722000S
Water 125mg/L Tank #4
N02
0.0348
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
. HB0722000ft
WatRr 125mo/L Tank f4
ND3
65 7
mnfl
4 427
A45TH1F
7/75/?non
Ren
Hook.
7/?3Wnnfl
Ren
Pfvilr
HB07220009
Water 250mg/L Tank #1
NQ2
0.0220
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07220009
Water 250mg/L Tank #1
N03
169.6
mg/L
11,0675
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
/ HB07220010
Water 250mg/L Tank #2
N02
0.0210
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
/ HB07220010
Water 250mg/L Tank #2
N03
173.2
mg/L
11.0676
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
^ HB07220011
Water 250mg/L Tank #3
N02
0.0220
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07220011
Water 250mg/L Tank #3
N03
168.8
mg/L
11,0675
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
HB07220012
Water 250mg/L Tank #4
N02
0.0230
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07??0017
Water ?50mn/L Tank #4
NQ3
173 4
ma/1
11 0675
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
HB07220013
Water 500mg/L T ar»k #1
N02
0.0273
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07220013
Water 500mg/L Tank #1
N03
425
mg/L
44.27
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
, HB07220014A
Water 5Q0mg/L Tank #2
N02
0.0171
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
f HB07220014A
Water 500mg/L Tank #2
N03
443
mg/L
44.27
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
%/ HB07220014B
Water SOOmg/L Tank #2
N02
0.01S4
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07220014B
Water 500mg/L Tank #2
N03
421
mg/L
44.27
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
HB07220015
Water 5Q0mg/L Tank #3
N02
0.0207
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07220015
Water 500mg/L Tank #3
N03
391
mg/L
44.27
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
HB07220016
Water 500mg/L Tank #4
N02
0.0187
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
J8
HB07220016
Water 5OOm0/L Tank #4
N03
409
ma/L
44.27
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
/ HBO722O017
Water 1000nng/L Tank #1
N02
0.0273
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
/ HB07220017
Water 1000mg/t Tank #1
N03
828
mg/L
110.675
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
/ HB07220018
Water 1000mg/L Tank #2
N02
0.0223
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
«4 HB07220018
Water 1000mg/L Tank #2
N03
744
mg/L
110.675
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
HB07220019
Water 1000mg/L Tank #3
N02
0.0289
mg/L
0.016425
A45008
7/23/2009
7/22/2009
JB
7/23/2009
JB
HB07220019
Water 1000mg/L Tank #3
N03
760
mg/L
110.675
A4500F
7/23/2009
7/22/2009
Ben
Cook
7/23/2009
Ben
Cook
HB07220020
Water tOOOmg/L Tank #4
N02
0.0220
mg/L
0.016425
A4500B
7/23/2009
7/22/2009
JB
7/23/2009
JB
- HRt)77?(m70
Watprin(lfln^i/1 Tank #4
N03
700
m
-------
Nitrate Day-2 FHM 32 Day Analytical Results 7.22.09
HB07220024
Water 2000mg/L Tank #4
N03
1950
mo/L
221.35
A4500F
7/23/2009
7/??;?009 BfinCrtnk
7/P3ffOfB»
Rpn Cnnk
HB07220025
Stock Solution 59.4g/L
N02
0,0069
mg/L
0.016423 J
A4500B
7/23/2009
7/22/2009 JB
7/23/2009
JB
HB07220025
Stock Solution 59.4g/L
N03
4S400
mgrt.
11067.5
A4500F
7/23/2009
7/22/2009 Ben Cook
7/23/2009
Ben Cook
HB07220026
Stock Solution 237.6g/l
N02
0.0194
mg/L
0.016425
A4500B
7/23/2009
7/22/2009 JB
7/23/2009
JB
HB07220026
Stock Solution 237.6g/L
N03
181200
mg/L
22135
A4500F
7/23/2009
7/22/2009 Ben Cook
7/23/2009
Ben Cook
Page 2
-------
Nitrate Day-1 FHM 32 Day Analytical Results 7.23.09
'» /LabSamplelD
SampleDescripti'on
PararnID
Results
Units
RL LabQualifier
AnalysisMethod
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Person
jiS>HB07230Q27
Dechlor Waterf Dilution Water
N02
0,0053
mg/L
0.016425 J
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
* {HB07230027
Dechlor Water/ Dilution Water
N03
0,980
mg/L
0.08854
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
MhB0723Q028
Water Control Tank #1
N02
0.0276
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
/ HB0723Q028
Water Control Tank #1
N03
0.974
mg/L
0.08854
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
/ HB07230029
Water Control Tank #2
N02
0.0283
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
1 HB07230Q29
Water Control Tank #2
N03
0.9831
mg/L
0.08854
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
HB07230030
Water Control Tank #3
N02
0.0348
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230030
Water Control Tank #3
N03
1.001
mg/L
0.08854
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
HB07230031
Water Control Tank #4
N02
0.0223
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230031A
Water Control Tank #4
N03
0.8261
mg/L
0.08854
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
HB07230031B
Water Control Tank #4
N03
0.9B7
mg/L
0.08854
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
HB07230032
Water 125mg/L Tank#1
N02
0.0296
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230032
Water 125mg/L Tank#1
N03
69.2
mg/L
4.427
A450QF
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
, HB07230033
Water 125mg/L Tank#2
N02
0.0260
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
1 HB07230033
Water 125mg/LTank#2
N03
72.1
mg/L
4.427
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
4 HB07230034A
Water 125mg/L Tenk#3
N02
0.0260
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
H807230034A
Water 125mg/L Tank#3
N03
86.7
mg/L
4.427
A450QF
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
HB072300346
Water 125mg/L Tank#3
N02
0.0283
mg/L
0.016425
A450QB
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230034B
Water 125mg/L T ank#3
N03
70.4
mg/L
4.427
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
HB07230035
Water 125mg/L Tank#4
N02
0.0256
mg/L
0.018425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230035
Water 125mfl/L Tank#4
N03
69.0
mg/L
4,427
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
HB07230036
Water 250mg/L Tank#1
NQ2
0.0243
mg/L
0,016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230036
Water 250mg/L Tankfft
N03
200.0
mg/L
11.0675
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
j HB07230037
Water 250mg/L Tank#2
N02
0,0269
mg/L
0.016425
A4500B
7/23/2008
7/23/2009 JB
7/23/2009
JB
S/ HB07230037
Water 2S0mg/L Tank#2
N03
180.1
mg/L
11.0675
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
HB07230038
Water 250mgfl_ Tank#3
N02
0,0315
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230038
Water 250mg/L Tank#3
N03
232.9
mg/L
11.0675
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
HB07230039
Water 250mg/L Tank#4
N02
0.0283
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HR07Mnrcwi
Water PHlmn/L Tank#*
N03
190.1
mo/L
11.0675
A4500F
7/23/2009
7/23/2009 Ben Cook
7/23/2009
Ben Cook
HB07230040
Water 500mg/L Tank#1
N03
455
mg/L
44.27
A4500F
7/24/2009
7/23/2009 Ben Cook
7/24/2009
Ben Cook
HB07230040
Wafer 500mg/L Tank#1
NQ2
0.0302
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
/ HB0723DQ40M
Water SOOmg/L Tank#1
N02
0.0933
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
/ HBQ7230041
Water SOOmg/L Tank#2
N03
472
mg/L
44.27
A4500F
7/24/2009
7/23/2009 Ben Cook
7/24/2009
Ben Cook
* HB0723OO41
Water 500mg/L Tank#2
N02
0.0237
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230042
Water SOOmg/L Tank#3
N03
470
mg/L
44.27
A4500F
7/24/2009
7/23/2009 Ben Cook
7/24/2009
Ben Cook
HB07230042
Water 500mg/L Tank#3
N02
0.0273
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230043
Water 500mg/L T arik#4
N03
522
mg/L
44.27
A4500F
7/24/2009
7/23/2009 Ben Cook
7/24/2009
Ben Cook
¦ HBn733nflAS
WfltBr snnmg/l TankOd
NO?
0.0276
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230Q44A
Water 1Q00mg/L Tank#1
N03
697
mg/L
221.35
A4500F
7/24/2009
7/23/2009 Ben Cook
7/24/2009
Ben Cook
HB07230044A
Water 1000mg/L Tank#1
N02
0.0302
mg/L
0,016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230044B
Water 1Q00mg/LTank#1
N03
780
mg/L
221.35
A4500F
7/24/2009
7/23/2009 Ben Cook
7/24/2009
Ben Cook
/ HB07230044B
Water 1000mg/LTank#1
N02
0.0273
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
J HB07230045
Water lOOOmg/LTank#2
N03
930
mg/L
221.35
A4500F
7/24/2009
7/23/2009 Ben Cook
7/24/2009
Ben Cook
HB07230045
Water 1000mg/L Tank#2
N02
0.0361
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230046
Water 1000mg/L Tank#3
N03
790
mg/L
221,35
A4500F
7/24/2000
7/23/2009 Ben Cook
7/24/2009
Ben Cook
HB07230046
Water 1000mg^-Tank#3
N02
0.0283
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230047
Water lOOOmgrt. Tank#4
N03
820
mg/L
221.35
A4500F
7/24/2009
7/23/2009 Ben Cook
7/24/2009
Ben Cook
HB07230047
Water 1OOQmn/L Tank#4
N02
0.0263
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
/ HB07230048
Water 2000mg/L Tank#1
N03
2020
mg/L
442.7
A4500F
7/24/2008
7/23/2009 Ben Cook
7/24/2009
Ben Cook
/ HB07230048
Water 20OQmg/L Tank#1
N02
0.0302
mg/L
0.018425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
4 HB0723004S
Water 2000mg/L Tank#2
N03
1640
mg/L
442.7
A4500F
7/24/2009
7/23/2009 Ben Cook
7/24/2009
Ben Cook
HB07230049
Water 2000mg/L Tank#2
N02
0.0260
mg/L
0.016425
A4500B
7/23/2009
7/23/2009 JB
7/23/2009
JB
HB07230050
Water 2000mg/L T ank#3
N03
1820
mg/L
442,7
A4500F
7/24/2009
7/23/2009 Ben Cook
7/24/2009
Ben Cook
Page 1
-------
Nitrate Day-1 FHM 32 Day Analytical Results 7.23.09
HB07230050
Water 2000mg/L T ank#3
N02
0.0384
mg/L
0.016425
A4500B
7/23/2009
7/23/2009
JB
7/23/2009
JB
HB07230051
Water 2000mg/L T ank#4
N03
1820
mg/L
442.7
A4500F
7/24/2009
7/23/2009
Ben Cook
7/24/2009
Ben Cook
HB0723OO51A
Water 2000mg/L Tank#4
N02
0.0378
mg/L
0.016425
A4500B
7/23/2009
7/23/2009
JB
7/23/2009
JB
HB07230051B
Water 2000mg/L Tank#4
N02
0.0338
mg/L
0.016425
A4500S
7/23/2009
7/23/2009
JB
7/23/2009
JB
Page 2
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Nitrate Day 0 FHM 32 Day Analytical Results 7.24.09
J
LabSamplelD SampleDescriptiori RaramlD
HB07240066 Water control Tank #1 -DayO N02
HB072400G6 Water control Tank #1-DayO N03
HB07240067 Water control Tank #2-Day0 N02
HB07240067A Water control Tank #2-Day0 N03
MB07240067B Water control Tank #2-Day0 N03
HB07240068 Water control Tank #3-DayO N03
HB07240068A Water control Tank #3-DayO N02
HB07240068B Water control Tank #3-DayO N02
HB07240069 Water control Tank #4-OayO N02
HB07240069 Water control Tank #4-PavO N03 _
Results Units
0.0141
1.046
0.0269
1.103
1.118
1.104
0.0141
0.0122
0.0122
AQ.6?.
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
ma/L
RL LabQualifier
0.016425 J
0.08854
0.016425
0.08854
0.08854
0.08854
0.016425 J
0.016425 J
0,016425 J
0.08854
AnalysisMethod
A4500B
A4500F
A4500B
A4500F
A450QF
A4500F
A4500B
A4500B
A4500B
A4500F
AnalysisOate
7/24/2008
7/24/2009
7/24/2008
7/24/2009
7/24/2Q0S
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
SampleDate
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
Analyst
JB
Ben Cook
JB
Ben Cook
Ben Cook
Ben Cook
JB
JB
JB
Ben Cook
Calibration Date
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
Calibration Person
JB
Ben Cook
JB
Ben Cook
Ben Cook
Ben Cook
JB
JB
JB
Ben Cook
J
J
J
HB07240070 Water 125mg/L nitrate Tank #1 -Day N02
HB07240070 Water 125mg/L nitrate Tank #1 -Day N03
HB07240071 Water 125rng/L nitrate Tank #2-Oay N02
HB07240071 Water 125mg/L nitrate Tank #2-Day N03
HB07240072 Water 125mg/L nitrate Tank #3-Day N02
HB07240072 Water 125m g/L nitrate Tank #3-Oay N03
HB07240073 Water 125mg/L nitrate Tank #4-Day N02
HB07240073 Water 125ma/L nitrate Tank #4-Dav NQ3
0.0174
54.1
0.0115
55.8
0.0164
62.3
0.0082
58.8
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
mg/L
0,016425
11.0675
0.016425 J
11.0675
0.016425 J
11.0675
0.016425 J
11.0675
A4500B
A4500F
A4500B
A4500F
A4500B
A4500F
A4500B
A4500F
7/24/2003
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
JB
Ben Cook
JB
Ben Cook
JB
Ben Cook
JB
Ben Cook
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
7/24/2009
JB
Ben Cook
JB
Ben Cook
JB
Ben Cook
JB
Ben Cook
HB07240074A Water 250mg/L nitrate Tank #1 -Day N02 0.0122 mg/L 0.016425 J A450OB 7/24/2009 7/24/2009 JB 7/24/2009 JB
HB0724D074A Water 250mg/L nitrate Tank #1 -Day N03 180.7 mg/L 11.0675 A4600F 7/24/2009 7/24/2009 Ben Cook 7/24/2009 Ben Cook
HB07240074B Water 250mg/L nitrate Tank #1 -Day NQ2 0.0141 mg/L 0,016425 J A4500B 7/24/2009 7/24/2009 JB 7/24/2009 JB
HB0724D0748 Water 250mg/L nitrate Tank #1 -Day N03 178.3 mg/L 11.0675 A4500F 7/24/2009 7/24/2009 Ben Cook 7/24/2009 Ben Cook
HB07240075 Water 250mg/L nitrate Tank #2-Day N02 0.0164 mg/L 0.016425 J A4500B 7/24/2009 7/24/2009 JB 7/24/2009 JB
HB07240075 Water 250mg/L nitrate Tank #2-Day N03 179.0 mg/L 11.0675 A4500F 7/24/2009 7/24/2009 Ben Cook 7/24/2009 Ben Cook
HB07240076 Water 250mg/L nitrate Tank #3-Day N02 0.0069 mg/L 0.016425 J A4500B 7/24/2009 7/24/2009 JB 7/24/2009 JB
HB0724007B Water 250mg/L nitrate Tank #3-Day N03 177.7 mg/L 11.0075 A4500F 7/24/2009 7/24/2009 Ben Cook 7/24/2009 Ben Cook
HB07240077 Water 250mg/L nitrate Tank #4-Day N02 0.0112 mg/L 0.016425 J A4500B 7/24/2009 7/24/2009 JB 7/24/2009 JB
HB07240077 Water 250mo/L nitrate Tank #4-Dav NQ3 184.6 ma/L 11.0675 A4500F 7/94/9009 7/?4
-------
Nitrate DayO FHM 32 Day Analytical Results 7.24,09
HB07240089 Water 2000mg/L nitrate Tank #4-Ds N03 2051 mg/l 110.675 A4500F 7/24/2009 7/24/2009 Ben Cook 7/24/2009 Ben Cook
Page 2
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Nitrate Day 3 FHM 32 Day Analytical Results 7.27.09
I LabSamplelD
-4 HB07270102
HB07270102
J
SampleDesoription
Overlying Water Tank#1
Overlying Water TanMH
Overlying Water Tank#1
ParamID
Control-Da N03
Control-Da N02
Results Units RL
LabQualifier
1.010
0.0105
mg/L 0.08854
mg/L 0.016425 J
AnalysisMethod
A4500F
A4500B
AnalysisDate
7/27/2009
7/27/2009
SampleDate
7/27/2009
7/27/2009
Analyst
Ben Cook
JB
Calibration Date
7/27/2009
7/27/2009
Calibration Person
Ben Cook
JB
HBO7270103
HB07270103
Overivlna Water Tank#1 125mo/L-DNQ2
74,3 mg/L
0.0427 mg/L
g J4J35' ¦
0.016425
-MH50F"
A4SOOB
"772772009"
7/27/2009
-rmrnrn
7/27/2009
"Ben Cook
JB
772772009"
7/27/2009
ben uook
JB
HB07270104A
HB07270104A
,H807270104B
HB07270104B
Overlying Water Tank#1
Overlying Water Tank#1
Overlying Water Tank#l
Overlying Water Tank#1
500mg/L-C N03
500mg/L-C N02
500mg/L-C N03
SOOmg/L-C NQ2
505 mg/L
0.0108 mg/L
452 mg/L
0.0102 mg/L
22.135
0.016425 J
22.135
0.016425 J
A45Q0F
A4500B
A4500F
A4500B
7/27/2009
7/27/2009
7/27/2009
7/27/2009
7/27/2009
7/27/2009
7/27/2000
7/27/2009
Ben Cook
JB
Ben Cook
JB
7/27/2009
7/27/2009
7/27/2009
7/27/2009
Ben Cook
JB
Ben Cook
JB
HB07270105
HB07270105
Overlying Water Tank#1
Overlying Water Tank#1
2000mg/l- N03
2000mg/L- NQ2
2149 mg/L
0.0154 mg/L
110.675
0.016425 J
A4500F
A4500B
7/27/2009
7/27/2009
7/27/2009
7/27/2009
Ben Cook
JB
7/27/2009
7/27/2009
Ben Cook
JB
Page 1
-------
Nitrate Day 5 FHM 32 Day Analytical Results 7.29.09
J
t
LabSamplelD SampleDescription ParamID Results Units RL LabQualifier AnalysisMethod AnalysisDate SampleDate Analyst Calibration Date Calibration Person
HB07290120 Overlying water tank #2 control-Day NQ3 1.380 mgfl. 0.08854 A4S00F 7/31/2009 7/29/2009 Ben Cook 7/31/2009 Ben Cook
HB07290120 Overlying water tank #2 control-Day NQ2 <0.0038 mg/L 0.016425 U A45Q0B 7/31/2009 7/29/2009 Ben Cook 7/31/2009 _ Ben Cook
t
HB07290121
HB07290121
Overlying water tank #1 250mg/L-Di NOS
Overlying water lank #1 250mg/L-Di NQ2
232.0 mgfl. 11.0675
<0.0036 mafl- 0,01_6425 UL
A4500F
A4500B
7/31/2009
7/31/2009
7/29/2009
7/29/2009
Ben Cook
Ben Cook
7/31/2009
7/31/2009
Ben Cook
Ben Cook
$
HB07290122 Overlying water tank #1 lOOOmg/L-C N03
llB07290122 Overlying water tank #1 IQOOmo/L-t NQ2
1098 mg/L 44.27
<0.0038 ma/L 0.016425 U
A4500F
A4500B
7/31/2009
7/31/2009
7/29/2000
7/29/2009
Ben Cook
Ben Cook
7/31/2009
7/31/2009
Ben Cook
Ben Cook
HB07290123
HB07290123
Stock solution 59.4 g/L NaN03 43.3 N03
Stock solution 59.4 g/L NaN03 43.3 N02
41900 mg/L
<0.0036 mg/L
2213.5
0.016425 U
A4500F
A4500B
7/31/2009
7/31/2009
7/29/2009
7/29/2000
Ben Cook
Ben Cook
7/31/2009
7/31/2009
Ben Cook
Ben Cook
Page 1
-------
Nitrate Day 7 FHM 32 Day Analytical Results 7.31,09
J
Tt,
LabSamplelD
HB07310124
H807310124A
HB07310124B
SampleDescription
Overlying water tank #3
Overlying water tank #3
Overlying water tank ff3
ParamlD
control-Day N02
control-Day NQ3
control-Day NQ3
Results Units RL
LabGualifier
0.0046 mg/L Q.016425 J
1.276 mg/L 0 08854
1.272 mg/L 0.08854
AnalysisMethod
A4500B
A4500F
A450OF
AnalysisDate
7/31/2009
7/31/2009
7/31/2009
SampieDate
7/31/2009
7/31/2009
7/31/2009
Analyst
Ben Cook
Ben Cook
Ben Cook
Calibration Date
7/31/2009
7/31/2009
7/31/2009
Calibration Person
Ben Cook
Ben Cook
Ben Cook
1
HB07310125
HR073ini?S
Overlying water tank #2
Ovsrtvino water tank #2
125rng/L-D< N03
|g5mg/L-Pi,MQ2
105.7 mg/L
„ Q.QQ69 ma/l
4.427
0 016425 .I
A4500F
ft45Q0S
7/31/2009
7/31I220S
7/31/2009
.7/3112009
Ben Cook
Ben Cook
7/31/2009
7/31/2PW
Ben Cook
Ben Cook
31
HB07310126
'HB07310126
Overlying water tank #2
Ovartvino water tank #2
500mg/l-Di NOS
500ma/L-Dt NQ2
476 mg/L 22.135
0.0141 fflO/L 0.016425 J
A4500F
A4500B
7/31/2009
7/31/2009
7/31/2009
7/31/2009
Ben Cook
Ben Cook
7/31/2009
7/31/2009
Ben Cook
Ben Cook
HB07310127
HB07310127
Overlying water tank #2
Overlying water tank #2
2000mg/L-C N03
2000mg/L-C N02
2056 mg/L
<0.0036 mg/L
110,675
0.016425 U
A4500F
A4500B
7/31/2009
7/31/2009
7/31/2009
7/31/2009
Ben Cook
Ben Cook
7/31/2009
7/31/2009
Ben Cook
Ben Cook
Page 1
-------
Nitrate Day 10 FHM 32 Day Analytical Results 8.3.09
J
t
LabSsmplelD SampleDescription ParamID
HB08030134 overlying water tank #4 control-Day N02
HB08030134A overlying water tank #4 control-Day N03
HB08030134B overiying water tank #4 control-Day N03
HB08030135 overlying water tank #2 250mg/L-Da n63
HB08030135 overlying water tank #2 250mg/L-Da N02
Hb68030136 overlying water tank #2 1000mg/L-D N03
HB08030136 overlying water tank #2 lOQOmg/L-D N02
Results Units RL LabQualifier AnalysisMethod AnalysisDate SampleDate Analyst Calibration Date Calibration Person
0.0072 mg/L 0.016425 J A4500B 8/3/2009 8/3/2009 JB 8*3/2009 JB
1.188 rng/L 0,08854 A4500F 8/3/2009 8/3/2009 Ben Cook 8/3/2009 Ben Cook
1.168 mg/L 0.08854 A4500F 8/3/2009 8/3/20Q9 Ben Cook 8/3/2009 Ben Cook
162 mg/L 22.135
0.0079 mgfl- 0.018425 J
A450OF
A4500B
8/3/2009
8/3/2009
8/3/2009 Ben Cook 8/3/2009
8/3/2009 JB 8/3/2009
Ben Cook
JB
1027 mg/L
0.0463 mg/L
35.416
0.018425
A45O0F
A4500B
8/3/2009
8/3/2009
8/3/2009 Ben Cook 8/3/2009
8/3/2009 JB 8/3/2009
Ben Cook
JB
Page 1
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Nitrate Day 12 FHM 32 Day Analytical Results 8.3.09
J
J
LabSamplelD
HB08050187
HB08050187A
HB08050187B
SampleDescription
overlying water tank
overlying water tank
overlying water tank
#3 control-Day 12
#3 control-Day 12
#3 control-Day 12
ParsmID Results Units
N03 1.179 mgfL
N02 <0.0036 mg/L
NQ2 <0.0036 mg/L
RL
LabQualifier
0.08854
0.016425 U
0.016425 U
AnalysisMethod AnalysisDate SampteDate Analyst Calibration Date Calibration Person
A4500F 8/7/2009 8/5/2009 Ben Cook 8/7/2009 Ben Cook
A4500B 8/6/2009 8/5/2009 Jl 8/6/2009 Ji
A4500B 8/6/2009 8/5/2009 Jl 8/6/2009 Ji
i
HB08Q5Q188
HB0805018B
overlying water tank
overlying water tank
#3 125mg/L-Oay 12
#3 125mo/L-Dav 12
N03
NQ2
95.9 mg/L 4.427
<0.0036 mo/L 0.016425 U
A4500F
A4500B
8/7/2009
8/6/2009
8/5/2009 Ben Cook 8/7/2009
_Sffl2fl28Jl SfflfflPTO
Ben Cook
J
*^#r
/ M
<4 HI
HB08050189
t«08050189A
IB(Jb050189B
HBO8050190
HB08050190
oveftying water tank #3 500mg/L-0ay 12 N03 443 mg/L 44.27
overlying water tank #3 500m9/L-Day 12 NQ2 <0.0036 mg/L 0.016425 U
A4500F
A4500B
8/7/2009
6/6/2009
8/5/2009 Ben Cook 8/7/2009
-JZ5i2QQSLJ mam-
Ben Cook
_J
overiying water tank
overlying water tank
overlying water tank
#3 500mg/L-Day 12
#3 2000mg/L-Day 12
#3 2000mg/L-Day 12
N02 <0.0036 mg/L 0,016425 U
N03 2183 mg/L 110.675
N02 0.383 mg/L 0.082125
A4500B
A4500F
A4500B
8/6/2008 8/5/2000 Ji 8/6/2009
8/7/2009 8/5/2009 Ben Cook 8/7/2009
8/6/2009 8/5/2009 jl 8/6/2009
Jl
Ben Cook
Jl
Page 1
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Nitrate Day 14 FHM 32 Day Analytical Results 8.7.09
J
¦j
LabSamplelD SampleDescription ParamiD Results Units RL LabQuaiifier
HB08070236 overiying water tank #2 control-Day N03 1.109 mg/L 0.08854
HB08070236 overlying water tank #2 control-Day NQ2 0.0055 mg/L 0.0t6425 J
Analysis Method AnalysisDate SampleDate Analyst Calibration Date Calibration Person
A4500F 8/7/2009 8/7/2009 Ben Cook 8/7/2009 Ben Cook
A45Q0B 8/7/2009 B/7/2009 .11 8/7/ymM J
HB0807023?
HB08Q70237
J HB08070238
HB0d070238
overiying water tank #3 250mg/L-Da N03 245.9 mg/L 11.0675 A4500F
overlying water tank #3 250mfl/L-Da NQ2 D.0066 moIt 0.016425 J A4500B
8/7/2009
8/7/2009
8/7/2009 Ben Cook 8/7/2009
srrn
-------
Nitrate Day 17 FHM 32 Day Analytical Results 8.10.09
J
LabSamplelD
HB081Q0250
HB08100250
$ HB08100251
HB08100251
"Z/HB0810Q252
•HB08100252
SampleDescription
overlying water tank #3
overlying water tank #3
ParamlD
control-Day N03
control-Day NQ2
Results Units
1.049 mg/L
0.0061 mfl/L
RL
LabQualifier
0.08854
0.016425 J
AnalysisMethod AnalysisDale SampleDate Analyst Calibration Date Calibration Person
A4500F 8/13/2009 8/10/2009 Ben Cook 8/13/2009 Ben Cook
A4500B 8/12/2009 8/10/2009 Jl 8/12/2009 JJ
overlying water tank #4
overlying water tank MA
125mg/L-DaN03
125mg/L-Da NQ2
V I
overlying water tank MA
overlying water tank
500mg/L-Da N03
SOOmq/L-Oa N02
78,5 mg/L
0.0099 mg/L
4.427
0.016425 J
A4500F
A450QB
8/13/2009
8/12/2009 ,
424 mg/L
0.0135 mg/L
22.135
0.016425 J
A450QF
A4500B
8/10/2009 Ben Cook 8/13/2009
,1/10/2009 Jl 8/12/2089
8/13/2009
8/10/2009 Ben Cook 8/13/2009
-MSfflmJ _M2ffiQQa_
Ben Cook
_JJ
Ben Cook
JJ
HB08100253
HB08110253
overlying water tank #4
overlying water tank #4
2000mg/L-D N02
2000mg/L-D NQ3
0,0205 mg/L
1922 mg/L
0.016425
110.675
A4500B
A4500F
8/12/2009
8/13/2009
8/10/2009 Jl 8/12/2009
8/10/200B Ben Cook 8/13/2009
Jl
Ben Cook
Page 1
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Nitrate Day 19 FHM 32 Day Analytical Results 8.10.09
J
LabSamplelD SampleDescription ParamlO Results Units RL LabQualifier
HB08120269 overlying water tank #4 control-Day N02 0.0113 mg/L 0.016425 J
HB08120269 overlying water tank #4 control-Dav NQ3 1.087 mart. D.088S4
AnatysisMethod AnalysisDate SampteDate Analyst Calibration Date Calibration Person
A45Q0B 8/12/2009 8/12/2009 Jt 8/12/2009 Jl
A4500F 8/13/2009 8/12/2009 Ben Cook 8/13/2009 Ben Cook
7
HB08120270 overlying water tank #4 250mg/L-Da N02
HB08120270 overlying water tank #4 250mq/L-Da NQ3
0.0314 mg/L 0.016425
187.9 mg/L 11.0675
A450QB
A45Q0F
8/12/2009 8/12/2009 Jl 8/12/2009
8/13/2003 8/12/2009 Ben Cook 8/13/2009
Jl
Ban Cook
HB08120271
HB08120271
overlying water tank #4 1000mg/L-D N02
overlying water tank #4 lOOOmg/L-D N03
0.0216 mg/L
1218 mg/L
0,016425
44.27
A45Q0B
A4500F
8/12/2009
8/13/2009
8/12/2009 Jl 8/12/2009
8/12/2009 Ben Cook 8/13/2009
Jl
Ben Cook
Page 1
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Nitrate Day 21 FHM 32 Day Analytical Results 8.14 09
j LabSamplefD Sample Description ParamID Results Units RL LabQualifier AnalysisMelhod AnalysisDate SampleDate Analyst Calibration Date Calibration Person
J HB081402B4 overlying water tank #1 control-Day N03 1,137 mg/L 0.08854 A4500F 8/14/2009 8/14/2009 Ben Cook 8/14/2009 Ben Cook
/ HB08140284A overlying water tank #1 control-Day N02 0.0125 mg/L 0.01642S J A450QB 8/14/2009 8/14/2009 Jl 8/14/2009 Jl
( HB08140284S overlying water lank #1 contnol-Dav NQ2 0.0145 ma/L 0.016425 J A4500B 8/14/2Q08 8/14/2009 Jl 8/14/2009 Jl
-------
Nitrate Day 24 FHM 32 Day Analytical Results 8.17.09
T
LabSamplelD SamploDescriptkin ParamiD
HB08170289 overlying water tank #2 control-Day N02
HB08170289A overling water tank #2 control-Day N03
H808170289B overlying water tank #2 contra-Day N03
Results Units RL LabQualifier AnalysisMsihod
0.0089 mg/L 0.016425 J A4500B
1.187 mg/L 0.08854 A4500F
1.156 mo/L 0.08854 A4500F
AnalyslsDate SampleDate Analyst Calibration Date Calibration Person
8/19/2009 8/17/2009 Jl 8/19/2009 Jl
8/17/2009 8/17/2009 Ben Cook a/17/2009 Ben Cook
6/17/2009 8/17/2009 Ban Conk B/17/POnfl BeH-Gook
J
HB08170290
HB08170290
overlying wa
overlying wa
er tank #1 250mg/L-Da
er tank #1 2S0mg/L-Pa
N03
N02
252 mg/L 22.135
0.1250 mg/L 0.016425
A4500F
A4500B
8/17/2009
8/19/2009
8/17/2009 Ben Cook
8/17/2008 Jl
8/17/2009
8/19/2009
Ben Cook
Jl
r
HB08170291 overlying water tank #1 1000mg/L-D N03
HB08170291 overlying water tank HI 1000mg/L-DNQ2
1190 mg/L 44.27
0.0161 mat 0.016425 J
A4500F
A4500B
8/17/2009
8/19/2009
8/17/2009 Ben Cook 8/17/2009
8/17/2009 Jl 8/19/2009
Ben Cook
Jl „
HB08170292
HB08170292
Stock solution 59,4g/L NaN03-30.0i
Stock solution 59.4g/L NaNO3-30.0i
N03
N02
46100 mg/L
0.0039 mg/L
2213.5
0.016425 J
A4500F
A4500B
8/17/2009
8/19/2009
8/17/2009 Ben Cook 8/17/2009
8/17/2009 Jl 8/19/2008
Ben Cook
Jl
Page 1
-------
Nitrate Day 26 FHM 32 Day Analytical Results 8.19,09
J
i
LabSamplelD Sample Description ParamID
HB08190302 overlying water tank #3 control-Day N02
HB08190302A overlying wafer tank #3 control-Day N03
HBQS190302B overlying water tank #3 control-Day NQ3
Results Units RL LabQualMier AnalysisMethod AnalysisDate
<0.0036 mg/L 0.016425 U A4500B 8/19/2009
1.098 mg/L 0,08854 A4500F 8/21/2009
1-151 mg/L 0.08854 A4500F 8/21/2009
SampleDate Analyst
8/19/2009 Jl
8/19/2009 Ben Cook
8/19/2009 Ben Cook
Calibration Date Calibration Person
8/19/200S Jl
8/21/2009 Ben Cook
8/21/2009 Ben Cook
i
HB08190303
HB08190303
overlying water tank #2125mg/L-Da N03
o me riving water tank #2 125mq/L-Da NQ2
105.8 mg/L 4.427
0.0101 ma/L 0.018425 J
A4500F
A4B00B
8/21/2009
8/19/2009
8/19/2009 Ben Cook
_8/19/2QW Jl
8/21/2009
8/19/2009
Ben Cook
I
HB08190304 overlying water tank #2 500mg/L-Da N03
HB08190304A overlying water tank #2 500mg/L-Oa N02
HB08190304B overlying water tank #2 SOOmg/L-Da NQ2
HB08190305
HB08190305
overt ying water tank #2 2000mg/L-D N03
overlying water tank #2 2000mg/L-D N02
599 mgrt- 22,135
0.0109 mg/L 0.016425 J
0.0092 mg/L 0.016425 J
A4500F
A4500B
A4500B
8/21/2009
8/19/2009
e/19/2009
8/19/2009 Ben Cook
8/19/2009 Jl
8/19/2005 Jl
8/21/2009
8/19/2009
R/ifl/?nna
Ben Cook
Jl
_j
2087 mg/L 110,675
0.0437 mg/L 0.016425
A4500F
A4500B
8/21/2009
8/19/2009
8/19/2009 Ben Cook
8/19/2009 Jl
8/21/2009
8/19/2009
Ben Cook
Jl
Page 1
-------
Nitrate Day 28 FHM 32 Day Analytical Results 8.21.09
J
7
LabSamplelD
HB0821Q321
HB08210321
Sample Description
overlying water tank
overlying water tank
ParamID
#4 control-Day N02
#4 control-Day NQ3
Results Units
<0.0036 mg/L
1.131 mg/L
RL
LabQualifier
0.016425 U
0.08854
AnalysisMethod AnalysisDale SampleDate Analyst Calibration Date Calibration Person
A45Q0B 8/21/2009 8/21/2009 Ben Cook 8/21/2009 Ben Cook
A4500F 6/21/2009 8/21/2009 Ben Cook 8/21/2009 Ben Cook
T
HB08210322
H808210322
HE08210323
HB08210323A
HB08210323B
overlying water tank
overlying water tank
#2 250mg/L-Da N02
«2 250mg/L-Qa NQ3
overlying water tank
overlying water tank
overlying water tank
#21000mg/L-D: N03
#21000mg/l-Di N02
#21000mg/l-Q< N02
0.0186 mg/L
273.6 mg/L
0.016425
11.0675
A4500B
A4500F
8/21/2009
8/21/2009
1191
1.33
1.35
mg/L
mg/L
mg/L
44.27
0.16425
0.18425
M50QF
A4500B
A4500B
8/21/2009 Ben Cook 8/21/2009
8/21/2009 Ben Cook 8/21/2009
Ben Cook
.Ben Cook
8/21/2009 8/21/2009 Ben Cook 8/21/2009 Ben Cook
8/21/2009 8/21/2009 Ben Cook 8/21/2009 Ben Cook
8/21/2009 8/21/2009 Ben Cook 8/21/2009 Ben Cook
Page 1
-------
Nitrate Day 32 FHM 32 Day Analytical Results 8.25.09
J
J
LabSamplelO SarnpleDescription ParamID Results Units
HBQ825Q357 overlying water tank #1 control Nitra N02 0.0072 mg/L
HB08250357A overlying water tank #1 control Nitra N03 <0.021 mg/L
HB08250357B overlying water tank #1 control Nitra N03 <0.021 mg/L
HB08250358 overtying water tank #2 control Nitra NQ2 0.0049 mg/L
HB08250358 overlying water tank #2 control Nitra N03 1.106 mg/L
HB08250359 overlying water tank #3 control Nitra N02 0.0087 mg/L
HB08250359 overlying water tank #3 control Nitra N03 1.047 mg/L
HB08250360 overlying water tank #4 control Nitra N02 0.0135 mg/L
HB08250360 overlying water tank #4 control Nitra NQ3 1.050 mq/L
RL LabQualifier
0.016425 J
0.08854 U
0.08854 U
0.016425 J
0.08854
0.016425 J
0.08854
0.016425 J
0.08854
AnalyslsMethod
A4500B
M500F
A4500F
A4500B
A4500F
A4500B
A4500F
A4500B
A4500F
AnalysisDate
8/25/2009
8/26/2009
8/26/2009
8/25/2009
8/26/2009
8/25/2009
8/26/2009
8/25/2009
8/26/2009
SampteDate
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
Analyst
Jl
Ben Cook
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Calibration Date
8/25/2009
8/26/2009
8/26/2009
8/25/2009
8/26/2009
8/25/2009
8/26/2009
8/25/2000
W26/2003
Calibration Person
Jl
Ben Cook
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
-BfinGQak
HB08250361 overlying water tank #1 125mg/L Nit N02 0,0076 mg/L 0.016425 J A4500B 6/25/2009 8/25/2009 Jl 8/25/2009 Jl
HB08250361 overlying water tank #1 125mg/L Nit N03 178.0 mg/L 4.427 A4500F 8/26/2009 8/25/2009 Ben Cook 8/26/2009 Ben Cook
HB08250362 overlying water tank #2125mg/L Nit N02 <0.0036 mg/L 0.016425 U A4500B 8/25/2009 8/25/2009 JI 8/25/2009 Jl
HB08250362 overlying water tank #2125mg/L Nit N03 181.4 mg/L 4.427 A4500F 8/26/2009 8/25/2009 Ben Cook 8/26/2009 Ben Cook
HB0825G363 overlying water tank #3125mg/L Nit N02 <0.0036 mg/L 0.016425 U A4500B 8/25/2009 8/25/2000 Jl 8/25/2009 Jl
HB08250363 overlying water tank #3125mg/L Nit N03 181.2 mg/L 4.427 A45Q0F 8/26/2009 8/25/2009 Ben Cook 8/26/2009 Ben Cook
HB08250364A overlying water tank #4125mg/L Nit N02 0.0131 mg/L 0.016425 J A4500B 8/25/2009 8/25/2009 Jl 8/25/2009 Jl
HB08250364A overlying water tank #4125mg/L Nit N03 181.0 mg/L 4.427 A4500F 8/26/2009 8/25/2009 Ben Cook 8/26/2009 Ben Cook
HB08250364B overlying water tank #4125mg/L Nit N02 0.0095 mg/L 0.016425 J A4500B 8/25/2009 8/25/2009 Jl 8/25/2009 Jl
HB08250364B overtying water tank #4 125mB/L Nit NQ3 180.7 mq/L 4.427 A4500F 8/26/2009 8/25/2009 Ben Cook 8/26/2009 Ben Cook
HB08250365 overlying water tank #1 250mg/L Nit N02 0.0122 mg/L 0.016425 J
HB08250365 overlying water tank #1 250mg/L Nit N03 285.6 mg/L 11.0675
HB08250368 overlying water tank #2 250mg/L Nit NQ2 0.0154 mg/L' 0.016425 J
HB08250366 overlying water tank #2 250mg/L Nit N03 295.9 mg/L 11.0675
HB08250367 overlying water tank #3 250mg/L Nit N02 0.0122 mg/L 0.016425 J
HBQ8250367 overlying water tank #3 250ing/L Nit N03 303.7 mg/L 11.0675
HB0825O368 overlying water tank #4 250mg/L Nit N02 <0.0036 mg/L 0.016425 U
HB08250368 overlying water tank 04 2S0mo/L Nit NQ3 309.5 roo/l 11 067S
A45Q0B
A4500F
A450QB
A4500F
A4500B
A4500F
A4500B
A45A0F
8/25/2009
8/26/2009
8/25/2009
8/26/2000
8/25/2009
8/26/2009
8/25/2009
ft/?fi/?M3
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
.RfjO-Cfink
8/25/2009
8/26/2009
8/25/2009
8/26/2009
8/25/2009
8/26/2009
8/25/2009
mm?tm
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
-J
HB08250369 overlying water tank #1 500mg/L Nit N02 0.0117 mg/L 0.016425 J A4500B
HB08250369 overlying water tank #1 500mg/L Nit N03 645 mg/L 44.27 A4500F
HB08250370 overlying water tank #2 500mg/L Nit N02 <0.0036 mg/L 0.016425 U A4500B
HB08250370 overlying water tank #2 500mg/L Nit N03 626 mg/L 44.27 A450QF
HB08250371 overlying water tank #3 500mg/L Nit N02 0.0095 mg/L 0.016425 J A4500B
HB08250371 overlying water tank #3 500mg/L Nit N03 605 mg/L 44.27 A4500F
HB08250372 overlying water tank #4 500mg/L Nit N02 0,0427 mg/L 0.016425 A4500B
HB08250372 overlying water tank #4 5QQmg/L Nit NQ3 615 mg/L 44.27 A4500F
7
8/25/2009
8/26/2009
8/25/2009
8/26/2009
8/25/2009
8/26/2009
8/25/2009
8/26/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
Jl
Ben Cook
Jl
Ban Cook
Jl
Ben Cook
Jl
Ben Cook
8/25/2009
8/26/2009
8/25/2009
8/26/2009
8/25/2009
8/26/2009
8/25/2009
8/26/2009
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
HB08250373 overlying water tank #1 1000mg/L N N02 0.014 mg/L 0.016425 J A4500B 8/25/2009 8/25/2009 Jl 8/25/2009 Jl
HB08250373 overlying water tank #1 lOOOmg/L N N03 1262 mg/L 44.27 A4500F 8/26/2009 8/25/2009 Ben Cook 8/26/2009 Ben Cook
HB08250374 overlying water tank S210OOmg/L N N02 2.40 mg/L 0.49275 A4500B 8/25/2009 6/25/2009 Jl 8/25/2009 Jl
HB08250374 overlying water tank #2 1000mg/L N N03 1327 mg/L 44.27 A4500F 8/26/2009 8/25/2009 Ben Cook 8/26/2009 Ben Cook
HS08250375 overlying water tank #3 1000mg/L N N02 0,0218 mg/L 0.016425 A4500B 8/25/2009 8/25/2009 Jl 8/25/2009 Jl
HB08250375 overlying water tank #31000mg/L N N03 1315 mg/L 44.27 A4500F 8/26/2000 8/25/2009 Ben Cook 8/26/2009 Ben Cook
HB08250376 overlying water tank #41000mg/L N N02 0.0158 mg/L 0.016425 J A4500B 8/25/2009 8/25/2009 Jl 8/25/2009 Jl
HB08250376 overlying water tank #4 IQOOroctfl. N NQ3 1316 ma/L 44.27 A4500F 8/26/2009 8/25/2009 Ben Cook 8/26/2009 Ben Cook
HB08250377 Stock solution 59.4g/L NaN03-43.3j N03 46100 mg/L 2213.5
HB08250377A Stock solution 59.4g/L NaN03-43,3< N02 0,0049 mg/L 0.016425 J
HB08250377B Stock solution 59.4g/L NaN03^3.3i N02 0.0049 mg/L 0.016425 J
A4500F
A4500B
A4500B
8/26/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
8/25/2009
Ben Cook
Jl
8/28/2009
8/25/2009
8/25/2009
Ben Cook
Jl
Jl
Page 1
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Page ' of
Qrcai Lates Environmental Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.com
Great Lakes Environmental Center
. . Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/22/09
Mailee Carton/Jamie Sax ton
m. garton
Bottle ID
DW-0A-25
DW-OA-26
DW-OA-27
DW-OA-28
DW-OA-29
DW-0A-30
Date / Time
of Sample
7/22/09
7/22/09
7/22/09
7/22/09
7/22/09
03<
7/22/09
U3&
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
125 ml.
Type of
Sample
Container
HOPE
IIDPE
HOPE
HOPE
HDPE
HDPE
Sample Description
(Site Information /
sample type)
Water control Tank #1
Water control Tank#2
Water control Tank #3
Water control Tank #4
Water 125mg/L
nitrate Tank #1
Water 125mg/L
nitrate Tank #2
Check the requested analysis (see definitions below)
Preservation
Cold
Cold
Cold
Cold
Cold
Cold
Shipper:
Date/Time:
n fiaxL O 11^/qI So
Receiver:
Date/Time:
I'D
Temperature:
BODs = Biochemical Oxygeit Demarfd:5"(lay
NHi-N = Ammonia-Nitrogen
NOVNOj-N = NilraU-ZNitrite-Nilrogeo
' Definitions
DS - lotal Dis -oKe So ic!s
TKN =r 'lotal Kjeldahl Nitrogen
TP = Total-Phosphorus"
TSS = Total Suspended Solids
*- "• - A&brcvtntioiis for utber frumenty reuuestcd analyses
NQz -N = Nitrite Nitrogen Total N = Total Nitrogen
|NO,-N = Nitrate Nitrogen SRF' = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page ^ of 5
Grsas Lakes Environmental Cemor
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/22/09
Mailce Garton/Jamie Saxton
m. garton
Bottle ID
DW-0A-31
DW-OA-32
DW-0A-33
DW-OA-34
DW-OA-35
DW-0A-36
Date / Time
of Sample
7/22/09
(33c
7/22/09
133*
7/22/09
\Q3o
7/22/09
7/22/09
P3®
7/22/09
\>J0
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
HDPE
Check the requested analysis (see definitions below i
Sample Description
(Site information I
sample type)
Preservation
Overlying water Tank
#3 125mg/L nitrate
Overlying water Tank
#4 125 mg/L nitrate
Overlying water Tank
#1 250 mg/L nitrate
Overlying water Tank
#2 250 mg/L nitrate
Overlying water Tank
#3 250mg/L nitrate
Overlying water Tank
#4 250mg/L nitrate
Shipper:
2.
Date/Time:
Receiver:
o
o/
Date/Time:
7-2? -O ^
Temperature:
NHj -N = Ammonia-Nitrogen
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen
Sufis
Suspended Solids
\hhn n.itiims fm nll.i; riiiiiioilh reonestpH.nnalvsi-c
' NO2 -N = Nitrite Nitrogen Total N = Total Nitrogm
NO? -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page of
Grsat Lakes Eirvlronrma^iai Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Traverse City, MI
Bottle ID
DW-OA-37
DW-0A-31
DW-OA-39
DW-0A-40
DW-OA-41
DW-OA-42
Date / Time
of Sample
7/22/09
7/22/09
7/22/09
123 ^
7/22/09
p?o
7/22/09
19 3o
7/22/09
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HOPE
1IDPE
HOPE
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
Overlying water Tank
#1 500mg/L nitrate
OYerlying water Tank
#2 500mg/L nitrate
Overlying water Tank
#3 500mg/L nitrate
Overlying water Tank
#4 500mg/L nitrate
Overlying water Tank
#1 lOOOmg/L nitrate
Overlying water Tank
#1 lOOOmg/L nitrate
Preservation
Cold
Cold
Cold
Cold
Cold
Cold
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/22/09
Mailee Gartcm/Jamie Saxton
m. garton
Shipper:
Date/Time:
Receiver:
2.
Date/Time:
7-23-0*1
Temperature:
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
11 '4 f ^3
Page of ^
Great La3«s Envfronrosniaf Ccntw
739 Hastings St,
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/22/09
Mai lee Garton/Jamie Sax ton
m. garton
Bottle ID
DW-OA-43
DW-OA-44
DW-OA-45
DW-OA-46
DW-0A-47
DW-OA-48
Date / Time
of Sample
7/22/09
1^3 o
7/22/09
7122IW
7/22/09
7/22/09
\Tjo
7/22/09
Volume
Collected
125 ml.
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
Overlying water Tank
#3 lOOOmg/L nitrate
Overlying water Tank
#4 lOOOmg/L nitrate
Overlying water Tank
#1 2000mg/L nitrate
Overlying water Tank
#2 2000mg/L nitrate
Overlying water Tank
#3 200Gmg/L nitrate
Overlying water Tank
#4 2000mg/L nitrate
__ Check the requested analysis (see definitions below)
Preservation
Cold
Cold
Cold
Cold
Cold
Cold
Shipper:
Date/Time:
r k u> %*(& (37?
Receiver:
4 12/
Date/Time:
Temperature:
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
or 5
GleC
Sfeal Lakes Efwironmentai Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T » Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/22/09
Mai lee Garton/Jamie Saxton
m. garton
Bottle ID
DW-0A-50
DW-OA-52
Date / Time
of Sample
7/22/09
1^70
7/22/09
(aio
Volume
Collected
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
U
Sample Description
(Site information /
sample type)
Stock solution 59.4
g/L Nitrate
Stock solution 237.6
g/L Nitrate
'djludh^ \\Q
Check the requested analysis (see definitions below)
Preservation
Cold"
Cold
W
Shipper:
2.
Date/Time:
1 l^O
Receiver:
I
A., ft*
Date/Time:
7-23-^
Temperature:
Total Dissolve
TKN = Total Kjeldahl Nitrogen - TSS = To«al Suspended Solid:.
8l Abbreviations for.other freaueailv rwinp«awl analnw.
NOj -N = Nitrite Nitrogen -Toul N = Total Nitrogen
NCh-N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
of
5-
GleC
Groal Lakes Environmental Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/22/09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
DW-0A-1
DW-OA-2
DW-OA-3
DW-OA-4
DW-0A-5
DW-OA-6
Date / Time
of Sample
7/22/09
Yl%o
7/22/09
{23Q
7/22/09
(230
7/22/09
123 a
7/22/09
03*
7/22/09
(Z%°
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPli
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
overlying water tank
#1 control
overlying water tank
#2 control
overlying water tank
#3 control
overlying water tank
#4 control
overlying water tank
#1 125 mg/L
overlying water tank
#2 125 mg/L
Cheek the requested analysis (see definitions below)
Preservation
h2so4
h,so4
h.so4
h,so4
h2so4
h2so4
Shipper:
Date/Time:
nf3>i^ 1330
Receiver:
Date/Time:
~?'73.~qA
Temperature:
BGDj = Biochemical Oxygen Dtniand-S day TDS = Total 1
NH3-N = Ammonia-Nitrogen TKN - Total Kjeldahl Nitrogen " TSS = Total Suspended Solids
NO3/NO2-N = Nitratf/Nitntc-Nitrogen
lie Nitrogen
NO--N = Nitrate Nitrogen
for other fittnicnth ifmifstfd aualts
Tofoil N = Total Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
2 of S
&&at tales Em'Jmrt rentes! Center
739 Hastings St,
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility;
Location:
Great Lakes Environmental Center
Traverse City, MI
Date;
Contact Person:
Email Address:
Phone Number:
Collector:
7/22/09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
DW-0A-7
DW-OA-
DW-0A-9
DW-OA-
10
DW-0A-
11
DW-0A-
12
Date /Time
of Sample
7/22/09
7/22/09
©3o
7/22/09
7/22/09
7/22/09
bip
7/22/09
p-K
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
overlying water tank
#3 125 mg/L
overlying water tank
#4 125 mg/L
overlying water tank
#1 250 mg/L
overlying water tank
#2 250 mg/L
overlying water tank
#3 250 mg/L
overlying water tank
#4 250 mg/L
Check the requested analysis (see definitions below
Preservation j <
H2SO„
h,so4
H2SO4
H2SO4
h2so4
h2so4
Shipper:
Date/Time:
ioAqa"1Lh i&q
Receiver:
A 0
Date/Time:
7-2l~oS
Temperature:
". :7""
-
Hf/imlmiis
BOD3 =' Biochemical Oxygen Demand-5 day
NHj-N = Ammooia-Nitrogen - _
N03/N02:N - Nitrate/Nitritc-Nitrogcn
n>S= lotdllh^v^SrliJs
TKN = Total Kjeldah] Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for othi'i fromienth nmiwted analvsw
NO2-N ="Nitrite~ Nitrogen ¦ Total N = Total Nitrogen
NO. -N = Nitrate Nitrogen SRI* = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page
3
of.
Great tafces EirwironrnerjisJ Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Pax: 231-941-2240
www.elec.com
Great Lakes Environmental Center
Facility:
, . Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/22/09
Mai lee Garton/Jamie Sax ton
m. garlon
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
DW-0A-
13
7/22/09
125 mL
HOPE
overlying water tank
#1 500 mg/L
h2so4
DW-OA-
14
7/22/09
125 mL
HOPE
overlying water tank
#2 500 mg/L
h2so4
DW-OA-
15
7/22/09
\93o
125 mL
HDPE
overlying water tank
#3 500 mg/L
H2S04
DW-OA-
16
7/22/09
P3D
125 mL
HDFE
overlying water tank
#4 500 mg/L
h2so4
DW-OA-
17
7/22/09
mo
125 mL
HDPE
overlying water tank
#1 1000 mg/L
h2so4
DW-QA-
18
7/22/09
125 mL
HDPE
overlying water tank
#2 1000 mg/L
h2so4
Shipper:
Date/Time:
— —
|gj
Receiver:
Date/Time:
7'7.1 -of
Temperature:
NHj-N = Ammonia-Nitrogen
NOj/NOj-N = Nitrate/Nitrile-Nitrogcn
TKN = 1 cilnI hielJahl Nitropcn TSS - total
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
M -5"
GleC
Groat Lakes Environmental Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax; 231-941-2240
www.Blec.com
Great Lakes Environmental Center
Facility:
r . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/22/09
Mai lee Garton/Jamie Saxton
m. garton
Bottle ID
DW-OA-
19
Date / Time
of Sample
7/22/09
m-o
Volume
Collected
125 ml.
Type of
Sample
Container
HDPE
Sample Description
(Site information /
sample type)
overlying water tank
#3 1000 rag/L
Preservation
h2so4
DW-OA-
20
7/22/09
125 mL
HDPE
overlying water tank
#4 1000 mg/L
H1SO4
DW-0A-
21
7/22/09
125 mL
HDPE
P3o
overlying water tank
#1 2000 mg/L
h2so4
DW-0A-
22
7/22/09
Q3>o
125 mL
overlying water tank
#2 2000 mg/L
h2so4
DW-0A-
23
7/22/09
(^3o
125 mL
HDPE
overlying water tank
#3 2000 mg/L
H2SO4
DW-0A-
24
7/22/09
125 mL
HDPE
overlying water tank
#4 2000 mg/L
H2SO4
Shipper:
2.
Date/Time:
^7/aa/o^ azo
Receiver:
A., QCJ
Date/Time:
*7-2? -01
Temperature:
Ahlw 1 tl.1U1.1u. for iiiIkt liitjiHiiiilv 1 HHH.-.U*! .unit
N = Nitrite Nitrogen ' Total N = Total Nitrogen
NOi N = Nitrate Nitrogen SRI* = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page,
of.
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/22/09
Mailee Gart on/Jamie Sax ton
rn, gart on
Bottle ID
DW-0A-
49
DW-0A-
51
Date / Time
of Sample
7/22/09
lD3o
7/22/09
~]|23K
Volume
Collected
125 mL
125 mL
U
Type of
Sample
Container
HDPE
HDPE
u
Sample Description
(Site information /
sample type)
stock solution 59.4
g/L
stock solution 237.6
g/L
be.cWo»* too
dAiiio'h
Check the requested analysis (see definitions below)
Preservation
h>so4
h2so4
u
Shipper:
Date/Time:
(230
Receiver:
0
Date/Time:
7 'Zl
Temperature:
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
UL
Page,
I o,q
GftnH tafros &ivuon,Tiffn!z»f O.-nter
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax:231-941-2240
mvw.elec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number;
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m. earton
j
j
v/l
J
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information I
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalinity
O
§
1:
6'
Chlorophyll a
Hardness
z
1
tn
35
z
t
1
CC
P
H
D-
H
0
v -.A
Nitrate
DW-OB-1
7/23/09
125 mL
HOPE
overlying water tank
#1 control
h2so4
X
DW-OB-2
7/23/09
125 mL
HDPE
overlying water tank
#2 control
HjS04
• .. •;
X
;:>r' '
DW-0B-3
7/23/09
125 mL
HDPE
overlying water tank
#3 control
h2so4
X
DW-OB-4
7/23/09
125 mL
HDPE
overlying water tank
#4 control
h2so4
¦ ....
x
DW-0B-5
7/23/09
125 mL
HDPE
overlying water tank
#1 125 mg/L
H2S04
5' •
-
• •-">
. ' ,K/">
x :
DW-OB-6
7/23/09
125 mL
HDPE
overlying water tank
#2 125 mg/L
h2so4
;;
- •- ..
X
/
A
Shipper:
Date/Time:
Receiver:
2.
1 /^-3/oc) i 3-• M D
Date/Time:
1-71,-01
Temperature:
Definitions
BODs = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NH3 -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS= Total Suspended Solids
NGj/NOj -N - Nitrate/Nitrite-Nitrogen
Abbreviations for other frequently requested analyses
N02 -N = Nitrite Nitrogen Total N = Total Nitrogen
NO, -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Pa:
ge ^ of H
Ore.-*? Lake-a Environments*! C^ntf.v
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.giec.eorn
Great Lakes Environmental Center
Facility:
r . Traverse City, MI
Location: }
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m. garton
V
V
/
V
J
J\
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below1
Bottle ID
Alkalinity j
a
O
S3
1 ( )
Chloride
Chlorophyll a
Hardness
Z
1
S3
Z
• ' et: :
z
--O"
z
CO
e
H
TKN' J
h
H
w
ca
H
u
¦**
Z .
DW-0B-7
7/23/09
125 ml
HDPE
overlying water tank
#3 125 mg/L
h2so4
x
DW-0B-8
7/23/09
125 mL
HDPE
overlying water tank
#4 125 mg/L
h2so4
-
X;"
DW-OB-9
7/23/09
125 mL
HDPE
overlying water tank
#1 250 mg/L
H2S04
X
DW-OB-IO
7/23/09
125 mL
HDPE
overlying water tank
#2 250 mg/L
h2so4
X
DW-OB-11
7/23/09
125 mL
HDPE
overlying water tank
#3 250 mg/L
h2so,
Xv
DW-OB-12
7/23/09
125 mL
HDPE
overlying water tank
#4 250 mg/L
h2so4
X-','-'
Date/Time:
1
2.
Shipper:
Receiver:
Date/Time:
Temperature:
' Definitions
BODj = Biochemical Oxygen Demand-5 day TDS «¦ Total Dissolve Solids TP = Total Phosphorus
NHj -N - Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS - Total Suspended Solids
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
NO3 -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page
of
Gmmi i-akc-s £;
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.gtec.com
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m, garton
\J
J
V
V
>/1
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
• £¦
j:
a
o
S3
" u-
: *° '
;s5
©
Chlorophyll a
: «S .
-an
}
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
1
of
GleC
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mai lee Garton/Jamie Saxton
m. garton
Check the requested analysis (see definitions below"
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Alkalinity
Q
O
m
4>
"Ea
0 r
¦ u :
Chlorophyll a
Hardness
&
I
53
z
sr.
t
¦0
0.
Z. '
w
a
H
H
a.
H
>'-m-
Nitrate !
/
DW-OB-19
7/23/09
125 mL
II DPR
overlying water tank
#3 1000 mg/L
h2so4
-
m'
4
DW-0B-20
7/23/09
125 mL
HDPE
overlying water tank
#4 1000 mg/L
h2so4
x; ^
DW-OB-21
7/23/09
125 mL
HOPE
overlying water tank
#1 2000 mg/L
h?so4
f
p,-:\
j
DW-0B-22
7/23/09
125 mL
HDPE
overlying water tank
#2 2000 mg/L
h2so4
-
if'- ">:¦ •'
-
x;:-:
V
DW-OB-23
7/23/09
125 mL
HDPE
overlying water tank
#3 2000 mg/L
h2so4
¦ '
X
V
DW-OB-24
7/23/09
125 mL
HDPE
overlying water tank
#4 2000 mg/L
h2so4
X-,";
Shipper:
t.
Date/Time:
ll-HO
Receiver:
4- M
2.
Date/Time:
Temperature:
BODs - Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen
¦ Definitions
IDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations forother frequently requested analyses
NOi -N - Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page \ of
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
. . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m, garton
V
V
V
/
J
J
Bottle ED
Date I Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below"
\
ti'-
Alkalinity
BOD,
Chloride
Chlorophyll a
Hardness
Z
m
(E
z
z.
--W
o
••-as ¦
¦ x
©
¦z
TDS
¦ I J
p-
«3
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of M
GleC
a"-''fi'#*-HasliBgs*"St.Co,,,'='
Traverse City, MI 49686
Phone: 231-941-2230
Fax; 231-941-2240
www.glec.cotn
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mai lee Garton/ Jamie Saxton
m. garton
7
J
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site Information I
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalinity.
O
O
ffl
Chloride
Chlorophyll a
Hardness >
¦ ¦ • :
z
X
z
1'
GO
0
H
-- -/v-
¦ '.-J:
H
t
Nitrite
DW-0B-31
7/23/09
125 mL
HDPE
Overlying water Tank
#3 125mg/L nitrate
Cold
X
DW-OB-32
7/23/09
125 mL
HDPE
Overlying water Tank
#4 125 mg/L nitrate
Cold
-----
-
X
i--:.
DW-OB-33
7/23/09
125 mL
HDPE
Overlying water Tank
#1 250 mg/L nitrate
Cold
¦
X
:
DW-OB-34
7/23/09
125 mL
HDPE
Overlying water Tank
#2 250 mg/L nitrate
Cold
,
X
>-• '
DW-0B-35
7/23/09
125 mL
HDPE
Overlying water Tank
#3 250mg/L nitrate
Cold
r -
S3';;
ill'.
X -
DW-0B-36
7/23/09
125 mL
HDPE
Overlying water Tank
#4 250mg/L nitrate
Cold
" '1
X
J
\/
N/
Shipper:
1.
2.
Date/Time:
Receiver:
a. QJ1
Date/Time:
7 -7J -ot
Temperature:
BODj = Biochemical Oxygen Dernand-5 day
NHj.-N = Ammonia-Nitrogen
NO3/NO2 -N = N i trate/Nitri te-N i trogen
Definition!
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations
for other freguentiv requested,analyses
NO2 -N = Nitrite Nitrogen
Total N = Total Nitrogen
NO3 -N = Nitrate Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page
3 0r_i
a «¦? iMHastingS'St? <"•>
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.gLec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m. garton
/
/
/
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek
the requested analysis (see definitions below)
AJkal nity
a
§
Chlorophyll a
i
Hardness
¦•"j- • - -- .
Z
K
Z,
,M-r,
M
-¦ o
¦15
: O
Z. '
\ to
:&
ty
: *¦*, •
• -r»tm = -
DW-OB-37
7/23/09
125 mL
HDPE
Overlying water Tank
#] 500mg/L nitrate
Cold
X:
DW-OB-38
7/23/09
125 mL
HDPE
Overlying water Tank
#2 500mg/L nitrate
Cold
Si#
X
DW-OB-39
7/23/09
125 mL
HDPE
Overlying water Tank
#3 500mg/L nitrate
Cold
r
¦ ..
XY;
¦ I •
DW-0B-40
7/23/09
125 mL
HDPE
Overlying water Tank
#4 500mg/L nitrate
Cold
• t-
:V'
-vV
:
X:,:
DW-OB-41
7/23/09
125 mL
HDPE
Overlying water Tank
#1 lOOOmg/L nitrate
Cold
- ¦:*:
•L 1-V&
X;.-
' - ;'Vv'
X
DW-OB-42
7/23/09
125 mL
HDPE
Overlying water Tank
#1 lOOOmg/L nitrate
Cold
X
/
A
Shipper:
1.
2,
Date/Time:
Receiver:
Date/Time:
-7 '71 -OS
Temperature:
Definitions
BODj = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NH3-N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NGj/NOj-.-'N' = Nitrate/Nitrite-Niirogen
NOj -N = Nitrite Nitrogen
NOj -N = Nitrate Nitrogen
Total N = Total Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page
of
awf $9°H&stmgs<$tP'¦ •«.
Traverse City. MI 49686
Phone:231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m. garton
J
4
¦J
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below1
+•».
*£
¦*5" .
£ -
< ~
o
o
m
Chloride
Chlorophyll a
Hardness
z
rt
a
z
¦*? -
r*
O
' "
-
fi
w
©
H
H
V)
so
H
Nitrite
DW-OB-43
7/23/09
125 mL
HDPE
Overlying water Tank
#3 1 OOOmg/L nitrate
Cold
X
DW-OB-44
7/23/09
125 mL
HDPE
Overlying water Tank
#4 1 OOOmg/L nitrate
Cold
-
DW-OB-45
7/23/09
125 mL
HDPE
Overlying water Tank
#1 2Q00mg/L nitrate
Cold
•
- ; t*
W.
DW-OB-46
7/23/09
125 mL
HDPE
Overlying water Tank
#2 2000mg/L nitrate
Cold
X :
DW-OB-47
7/23/09
125 mL
HDPE
Overlying water Tank
#3 2 OOOmg/L nitrate
Cold
-
DW-OB-48
7/23/09
125 mL
HDPE
Overlying water Tank
#4 20Q0mg/L nitrate
Cold
X
\i
4
Shipper:
1.
Date/Time:
I 3l" i-\ O
Receiver:
Date/Time:
I'll-*
Temperature:
BOD; = Biochemical Oxygen Demand-5 day
NHj-N = Ammonia-Nitrogen
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Definitions
IDS - Total Dissolve Solids
TKN = Total Kjcldahl Nitrogen
TP = Total Phosphorus
TSS= Total Suspended Solids
ABbreviationrfor other frequently requested analyses
NOj-N =Nitrite Nitrogen Total N = Total Nitrogen
NO} -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page
\ of H
Traverse City, MI 49686
Phone; 231-941-2230
Fax: 231-941-2240
www.Elec.com
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below1
Alkalinity
a
0
n
Chloride
Chlorophyll a
vVg-
Z
S3
Z
o-
z
1
a
' , ^ ' * >¦.
£
w
4>
c
'fc-
DW-0-25
7/24/09
125 mL
HDPE
Water control Tank #1
-Day 0
Cold
-O
"
f?.-'
X
DW-0-26
7/24/09
125 mL
HDPE
Water control Tank#2
-Day 0
Cold
x ,
DW-0-27
7/24/09
125 mL
HDPE
Water control Tank #3
-DayO
Cold
X
DW-0-28
7/24/09
125 mL
HDPE
Water control Tank #4
-Day 0
Cold
'•V-:;
X-
DW-0-29
7/24/09
125 mL
HDPE
Water 125mg/L
nitrate Tank ff 1 -Day 0
Cold
.
. -'v" '•••'
:: ;v< 1;.
X
DW-0-30
7/24/09
125 mL
HDPE
Water 125mg/L
nitrate Tank #2-Day 0
Cold
X ¦ -
Shipper:
Date/Time:
01 up
Receiver:
Date/Time:
Temperature:
Definitions
BODs = Biochemical Oxygen Demand-S day TDS = Total Dissolve Solids TP = Total Phosphorus
NM3 -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
N0j/NO2 -N = Nitratc/Nitritc-Nitrogen
Abbreviation* for other freqnentlv requested analyses
NO2 -N = Nitrite "Nitrogen Total N = Total Nitrogen
NO? -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
,/"~i
Facility:
Page
3-of M
a-«?§#jHastingS"St.Cc"K-'
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
, Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mai lee Garten/Jamie Saxton
m. garton
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
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is
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BODs
Chloride
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DW-0-31
7/24/09
125 mL
HOPE
Overlying water Tank #3
125mg/L nitrate-Day 0
Cold
- :v:-
X: ;.rf
DW-0-32
7/24/09
125 mL
HDPE
Overlying water Tank #4
125 mg/L nitrate-Day 0
Cold
3;.'^
X-
DW-0-33
7/24/09
125 mL
HDPE
Overlying water Tank #1
250 mg/L nitrate-Day 0
Cold
V ^
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DW-0-34
7/24/09
125 mL
HDPE
Overlying water Tank #2
250 mg/L nitrate-Day 0
Cold
.. ^
y -
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DW-0-35
7/24/09
125 mL
HDPE
Overlying water Tank #3
250mg/L nitrate-Day 0
Cold
=
x.';-;.
DW-0-36
7/24/09
125 mL
HDPE
Overlying water Tank #4
250mg/L nitrate-Day 0
Cold
x-
Shipper:
Date/Time:
im-fi caoq
Receiver:
^ (l/1
Date/Time:
7-IH-oS
Temperature;
PeflnltiOM .' '
BO^j Biuvhemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NH3-N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NO3/NO1 -N = Nitrate/Nitrite-Nitrogen
Abbreviati
NOj -N = Nitrite Nitrogen
NO3 -N = Nitrate Nitrogen
Total N = Total Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page of
^ of ^
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page [_ of
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^"€B94fcsltags
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Lfste-s €iTvir
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glee.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Gaiton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below
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-
-------
CHAIN OF CUSTODY
(To be completed and submitted
RECORD
with samples)
Page
U
<3 of f
CjLa
Gn;fil Lakes ConU-r
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below)
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-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Greol Lakos Enu1r«iniQi3?al
739 Hastings St
Traverse City, MI 49686
Phone: 231-941-2230
Fax; 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
. . Traverse City, MI
Location: '
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
H of ^
7/23/09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
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DW-0-19
7/24/09
125 mL
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overlying water tank
#3 1000 mg/L-Day 0
h2so4
•
X
DW-0-20
7/24/09
125 mL
HDPE
overlying water tank
#4 1000 mg/L-Day 0
H2S04
:
X
DW-0-21
7/24/09
125 mL
HDPE
overlying water tank
#1 2000 mg/L-Day 0
ILS04
_
X
DW-0-22
7/24/09
125 mL
HDPE
overlying water tank
#2 2000 mg/L-Day 0
h2so4
X
DW-0-23
7/24/09
125 mL
HDPE
overlying water tank
#3 2000 mg/L-Day 0
h2so4
...
-
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DW-0-24
7/24/09
125 mL
HDPE
overlying water tank
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h2so4
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2.
Date/Time:
llanL-q OTqq
Receiver:
ILM.
BODs = Biochemical Oxygen Demand-5 day
Nil, -N = Ammonia-Nitrogen
NOj/NOj-N " Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjcldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Date/Time:
7-2 V-o)
Temperature:
Abbreviations for otherfreaueiiitiv requested analyses
NOj -N - Nitrite Nitrogen Total N= Total Nitrogen
NO} -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY
(To be completed and submitted
GleC
RECORD
with samples)
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax:231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
I
Page * of *—
•jiV-'i f" ' 7 /!*?
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information I
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
Q
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DW-3-1
7/27/09
125 niL
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overlying water tank
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II2SO4
:
X
DW-3-2
7/27/09
125 mL
HOPE
overlying water tank
#1 control-Day 3
Cold
X
DW-3-3
7/27/09
125 mL
HDPE
overlying water tank
#1 125mg/L-Day 3
ILSO4
X
DW-34
7/27/09
125 mL
HDPE
overlying water tank
#1 125mg/L-Day 3
Cold
: v" •-
X
DW-3-5
7/27/09
125 mL
HDPE
overlying water tank
#1 500 mg/L-Day 3
HjSOi
X
DW-3-6
7/27/09
125 mL
HDPE
overlying water tank
#1 500 mg/L-Day 3
Cold
r':7*':tr
X
Shipper:
-V
1 u
Date/Time:
ifrrfotoi$
Receiver:
&>- Q *
Date/Time:
Temperature:
BOD5 = Biochemical Oxygen Dernand-5 day
NHj -N = Ammonia-Nitrogen
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS - Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N — Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page_
of
Gle(
EfiystDtii
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
yvww.giec.com
Great Lakes Environmental Center
Facility:
. . Traverse City, MI
Location; J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
-7/Z1 /t>1
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
{Site information /
sample type)
Preservation
Check the requested analysis (see definitions below"
1 1
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7/27/09
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DW-3-8
7/27/09
125 mL
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overlying water tank
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Date/Time:
"7- 11-C^
Temperature:
BODj = Biochemical Oxygen Demand-S day
NH3 -N - Ammonia-Nitrogen
NOj/NOi -N = Nitrate/Nitrite-Nitrogen
IDS = Total Dissolve Solids TP = Total Phosphorus
TKN - total KjeldaJi! Nitrogen TSS = Total-Suspended Solids
Abbreviations for oilier: frequently requested analyses
N02-N = Nitrite Nitrogen Total N = Total Nitrogen
NOs -N = Nitrate Nitrogen SRP « Soluble Reactive Phosphoni
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
739 Hastings St,
Traverse Citv, MI 49686
Phone; 231*-94l-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T .. Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page 1 of
7/23/09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below'
V Alkalinity
a
§
Chloride
Chlorophyll a
t
' z
1
tfl
a
H
CL
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Z
DW-5-1
7/29/09
125 niL
HDPE
overlying water tank
#2 control-Day 5
h2so4
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•i"'
X
DW-5-2
7/29/09
125 mL
HDPE
overlying water tank
#2 control-Day 5
Cold
-
K
DW-5-3
7/29/09
125 mL
HDPE
overlying water tank
#1 250mg/L-Day 5
H2S04
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DW-5-4
7/29/09
125 mL
HDPE
overlying water tank
#1 250mg/L-Day 5
Cold
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DW-5-5
7/29/09
125 mL
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overlying water tank
#1 1000 mg/L-Day 5
H2SO4
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X
DW-5-6
7/29/09
125 mL
HDPE
overlying water tank
#1 1000 mg/L-Day 5
Cold
hi.'-
X
Shipper:
Date/Time:
PODj = Biochemical Oxygen Demand-5 day
NH3-N = Ammonia-Nitrogen
NO5/NO2 -N = Nitrate/Nitrite-Nitrogen
ifzAfal 1)00
Receiver:
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP - Total Phosphorus
TSS = Total Suspended Solids
Date/Time:
7-It-'l
Temperature:
Abbreviatio
NO2 -N = Nitrite Nitrogen
NO] -N = Nitrate Nitrogen
Total N = Total Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
o-
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
, . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
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7/29/09
125 mL
HDPE
Stock solution 59.4
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h2so4
r
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:;-"V
DW-5-8
7/29/09
125 mL
HDPE
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cold
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Date/Time:
1100
Receiver:
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Date/Time:
7 -ZQ-uH
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NOj/NOi -N = Niirate/Nitritc-Nitiogcn
TDS = Total Dissolve -Solids TP = Total Phosphorus
TKN = Total Kjeldahi Nitrogen TSS = Total SuspendeiSolids
? Abbreviations forother frequently requested analyses
NOj -N - Nitrite Nitrogen Total N - Total Nitrogen
. NOj *N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY
(To be completed and submitted
RECORD
with samples)
Page
of
739 Hastings St,
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m. garton
Check the requested analysis (see definitions below1
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
.. ;
J*-*-" '
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DW-7-1
7/31/09
125 mL
HOPE
overlying water tank
#3 control-Day 7
H2SO.,
x;.\
DW-7-2
7/31/09
125 mL
HDPE
overlying water tank
#3 control-Day 7
Cold
X
DW-7-3
7/31/09
125 mL
HDPE
overlying water tank
#2 125mg/L-Day 7
M2SO4
X
DW-7-4
7/29/09
125 mL
HDPE
overlying water tank
#2 125mg/L-Day 7
Cold
X
DW-7-5
7/31/09
125 mL
HDPE
overlying water tank
#2 500 mg/L-Day 7
H2SO4
-.'vvVJ
y--.-.
x-; V.:
DW-7-6
7/31/09
125 mL
HDPE
overlying water tank
#2 500 mg/L-Day 7
Cold
X
Shipper:
Date/Time:
Receiver:
Date/Time:
*1 'If'Q f
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NO3/NG2 -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS= Total Suspended Solids
Abbreviations (brother frequently requested analyses
N02 -N = Nitrite Nitrogen Total N - Total Nitrogen
NO3 -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
/ \ ~>j(
CVesit uatea Eiivi
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
www, glee, com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date.
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below
>
1 Alkalinity
O
O
03
Chloride
Chlorophyll a
Hardness
Z
1
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X
z
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m
£
DW-7-7
7/31/09
125 mL
HDPE
overlying water tank
#2 2000 mg/L-Day 7
h2so4
X ;•
DW-7-8
7/31/09
125 mL
HDPE
overlying water tank
#2 2000 mg/L-Day 7
h2so4
"y-M.
X
s':
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.
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Shipper:
Date/Time:
Receiver:
I,.
2,
4- &
Date/Time:
l-V'o'h
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NOj/MOj -N = Nilrate/Nitrite-Nitrogen
Definitions
TDS - Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP - Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analysts
NOz -N = Nitrite Nitrogen Total N - Total "Nitrogen
NO3 -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax:231-941-2240
www.elec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
of
7/23/09
Mailee Garton/Jamie Sax ton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below]
¦ i?V
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Nitrate
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DW-10-1
8/3/09
125 mL
HOPE
overlying water tank
#4 control-Day 10
H2SO4
X; ¦
DW-10-2
8/3/09
125 rnL
HDPE
overlying water tank
#4 control-Day 10
Cold
X
DW-10-3
8/3/09
125 mL
HDPE
overlying water tank
#2 250mg/L-Day 10
HjS04
X/';
DW-10-4
8/3/09
125 rnL
HDPE
overlying water tank
#2 250mg/L-Day 10
Cold
X
DW-10-5
8/3/09
125 mL
HDPE
overlying water tank
#2 1000 mg/L-Day 10
h2so*
X !
DW-10-6
8/3/09
125 mL
HDPE
overlying water tank
#2 1000 mg/L-Day 10
Cold
X
Shipper:
jjy.
XtT"
Date/Time:
BOD? = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen
Receiver:
Date/Time:
ay-pi
Temperature:
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS - Total Suspended Solids
Abbreviations for other frequently reuuested analyses
NOj -N = Nitrite Nitrogen Total N ¦ Total Nitrogen
NO3 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
of
CjLEIw-
O'Qiit Uihc;,-. LnviiOi Co.-
739 Hastings St
Traverse City, MI 49686
Phone; 231-941-2230
Fax: 231-941-2240
wwv.glec.com
Great Lakes Environmental Center
Facility:
. . Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/5/09
Mailee Garton/Jamie Saxton
I!9WD
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
1
Alkalinity
"v.-
0
g
Chlorophyll a
Z
r*>
X
z
z
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0.
z
i
w
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(M ::
Oh
H
m
H
Nitrate
a
'u
z
DW/f-l
\>
8/5/09
125 ml
HDPE
overlying water tank
#3 control-Day 12
h2so4
. . ¦
x ;
DW-12-2
8/5/09
125 mL
HOPE
overlying water tank
#3 control-Day 12
Cold
feciix
fav-:
• ;; A' :-
X
DW-12-3
8/5/09
125 mL
HDPE
overlying water tank
#3 125mg/L-Day 12
h2so4
h:- '•
x
DW-12-4
8/5/09
125 mL
HDPE
overlying water tank
#3 125mg/L-Day 12
Cold
;*<
'iV-
j. ~.
X
DW-12-5
8/5/09
125 mL
HDPE
overlying water tank
#3 500 mg/L-Day 12
H2SO4
.....A
V-- .•
¦ - r./'A"
W-:"-
DW-12-6
8/5/09
125 mL
HDPE
overlying water tank
m 500 mg/L-Day 12
Cold
A.-
X
Shipper:
Date/Time:
Receiver:
BOD) = Biochemical Oxygen Demand-S day
NH3 -N = Ammonia-Nitrogen
NQ3/NO2 -N - Nitrate/Nitrite-Nitrogen
Date/Time:
Temperature:
Definition*
TDS = Total Dissolve Solids
TKN = Total Kjcldahl Nitrogen
TP = Total-Phosphorus
TSS= Total Suspended Solids
Abbreviations iFop otheB freouiiitlV reqnested analyses
N02 -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
-n
Facility:
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
vnvw.glec.com
Great Lakes Environmental Center
, Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/5/09
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information I
sample type)
Preservation
Check the requested analysis (see definitions below"
1
Alkalinity
a
§
. Chloride
Chlorophyll a
¦¦VI'
V
¦ c
*©-
cs
=
z
sc
z
Z '
o
z
to
a
-
&
H
vM
' H-
¦i-
z
DW-12-7
8/5/09
125 ml.
HDPE
overlying water tank
#3 2000 mg/L-Day 12
h2so4
: ' : *
.....
X.';'"
DW-12-8
8/5/09
125 ml
HOPE
overlying water tank
#3 2000 mg/L-Day 12
h>so4
f,"
•"'••/•J-'
X
- .
Date/Time:
Shipper:
Receiver:
Date/Time:
£ -4'Ol
Temperature:
—
BODj = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NOj/NOz -N = Nitrate/Nitrite-Nitrogen
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS= TotalSuspcnded Solids
NOi-N = Nitrite Nitrogen
NOj -N = Nitrate Nitrogen
Total N = Total Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
wmv.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mai lee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below;
•. "*-*¦
- "m':-
:
a
§
Chloride
Chlorophyll a
T
z
X
z
1
d
z
: O"
¦ Z
O
H
"V"
S-
t»
' 05
H
' Nitrate
Nitrite
DW-14-1
8/7/09
125 mL
HDPE
overlying water tank
#2 control-Day 14
h2so4
X
DW-14-2
8/7/09
125 mL
HOPE
overlying water tank
#2 control-Day 14
Cold
X
DW-14-3
8/7/09
125 mL
HDPE
overlying water tank
#3 250mg/L-Day 14
h2so4
:: : " ;
X ¦'
DW-14-4
8/7/09
125 mL
HDPE
overlying water tank
#3 250mg/L-Day 14
Cold
• "• •
V-
'
X
DW-14-5
8/7/09
125 mL
HDPE
overlying water tank
#3 1000 mg/L-Day 14
h2so+
X': •
DW-14-6
8/7/09
125 mL
HDPE
overlying water tank
#3 1000 mg/L-Day 14
Cold
¦f
X
Shipper:
1 ¦ CX~ 1fcf ft vij
2.
BODs = Biochemical Oxygen Demand-5 day
NHj -N - Ammonia-Nitrogen
NO3/NG2-N = Nitrate/Nitrite-Nitrogen
Date/Time:
%/?/e °t
Receiver:
Date/Time:
Temperature:
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -"N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
f—'f /-—-J
CjtLE!^
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Page
of
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date I Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below'
1
Alkalinity
Q
O
03
Chloride
1 V ¦
Chlorophyll a
Z
as
z
VI
a
H
h' ;
£
so
50
H
Nitrate
o>
-ts
Z
DW-14-7
8/7/09
125 mL
HDPE
Stock solution 59.4
g/L NaN03 -XK%/L
Nitratc
h2so4
X '
DW-14-8
8/7/09
125 mL
HDPE
Stock solution 59.4
g/L NaN03 -M^g/L
Nitrate
cold
' . " ¦
: ¦' ;-:c-; b
-:1. :
X
X:?'%
-
' ¦' - >'
sag
'--'i
Shipper: Date/Time: Receiver: Date/Time: Temperature:
1. CI , "Tv-g.. Yo i"A O 0 f 3-7-c
2.
- ¦ ¦ ¦ - -V. ¦¦ . .-y-rsr! """ - ¦
Definitions "
BODj = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj -N = Ammonia-Nitrogen TKN - Total Kjeldah! Nitrogen TSS = Total Suspended Solids
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen
Abbreviations for other frequently requested analyses
NO. -N - Nitrite Nitrogen Total N - Total Nitrogen
NO, -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.com
Great Lakes Environmental Center
Facility:
r .. Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page ^ of Q
~g|to [Of
Mailee Garton/Jamie Saxton
fVV QiPrrhfb-' I S-
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below) I
Bottle ID
"¦¦¦£%
¦
<.
r - *" -
M
O
O
a
Chloride
08
S
2
u
" R
ffl
z.
r»
¦X
z
*
1.
€
;z
OS
0
H'
CM
H
m
OS
H
Nitrate
Nitrite
DW-17-1
8/10/09
125 mL
HDPE
overlying water tank
#3 control-Day 17
h2so4
.....
DW-17-2
8/10/09
125 mL
HDPE
overlying water tank
#3 control-Day 17
Cold
X
DW-17-3
8/10/09
125 mL
HDPE
overlying water tank
#4 125mg/L-Day 17
H2SO4
X
DW-17-4
8/10/09
125 mL
HDPE
overlying water tank
#4 125mg/L-Day 17
Cold
"
X
DW-17-5
8/10/09
125 mL
HDPE
overlying water tank
#4 500 mg/L-Day 17
h2so4
DW-17-6
8/10/09
125 mL
HDPE
overlying water tank
#4 500 mg/L-Day 17
Cold
X
Shipper:
<3,
Date/Time:
(Qf^)Q
Receiver:
£ /y
Date/Time:
Temperature:
BODj - Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NOj/NOj-N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN - Total Kjcldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for ol
icr freauentlv rcouested analyses
NO2 -N = Nitrite Nitrogen
Total N = Total Nitrogen
NO3 -N = Nitrate Nitrogen
SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
('it fC"
V i l.A -.V—
739 Hastings St
Traverse City, MI 496S6
Phone: 231-941-2230
Fax; 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
. . Traverse City, MI
Location: 7
Page
^ of}
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
"Blio
Mailee Garton/Jamie Saxton
{Xrndt
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information 1
sample type)
Preservation
Check the requested analysis (see definitions below)
:.¦Aikaiipi.tyi1"-
a
o
oa
Chloride
Chlorophyll a
¦ tA
¦ un
'
L.
«
:
z
fi
35
Z
?r
o -
W.
. r»
• •©'<
: t5Bv .
to
«
H
' Z
el,
H
' ¦¦¦¦& ¦
s
«
J"#'
- -
-i-H
; &
4>
*-» •
wmm
Z
DW-17-7
8/10/09
125 mL
HDPE
overlying water tank
#4 2000 mg/L-Day 17
h2so4
E-."
DW-17-8
8/10/09
125 mL
HDPE
overlying water tank
#4 2000 mg/L-Day 17
i^MTHOT
X
Shipper:
Date/Time:
mJViJi, /vm
Receiver:
&J1CJ
Date/Time:
8-/f-o1
Temperature:
BODs = Biochemic-al Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NCb/NOj-N - Nitratc/N itrite-N itrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS= Total Suspended Solids
Abbreviations for other frequently requested analyses
N02 -N = Nitrite Nitrogen Total N = Total Nitrogen
N03-N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
wvvw.glec.com
F acility.
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
J of ^
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below'
Alkalinity
Q
O
ta
Chloride
Chlorophyll a
m
tti _¦
C -
¦ '-trn '
' <3&
PC
z.
PS
z
*? *
r
oa
Q
K
P»
H
.05
00
Nitrate
Nitrite
DW-19-1
8/12/09
125 niL
HOPE
overlying water tank
#4 control-Day 19
h2so4
X; ,
DW-19-2
8/12/09
125 mL
HDPE
overlying water tank
#4 control-Day 19
Cold
X
DW-19-3
8/12/09
125 mL
HDPE
overlying water tank
#4 250mg/L-Day 19
h2so4
X
DW-19-4
8/12/09
125 mL
HDPE
overlying water tank
#4 250mg/L-Day 19
Cold
X
DW-19-5
8/12/09
125 mL
HDPE
overlying water tank
#4 1000 mg/L-Day 19
h2so4
r .
x -
DW-19-6
8/12/09
125 mL
HDPE
overlying water tank
#4 1000 mg/L-Day 19
Cold
X
Shipper:
Date/Time:
ji.
XQ2.Q
Receiver:
Date/Time:
P-(7 -Cfi
Temperature:
BODj = Biochemical Oxygen Demand-5 day
NHj-N = Ammonia-Nitrogen
NOj/NOi-N = Nitrate/Nitrite-Nitrogen
' Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
F
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
N02 -N = Nitrite Nitrogen Total N = Total Nitrogen
N03 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
{To be completed and submitted with samples)
Page
Great Lakes Environmental Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mai lee Garton/Jamie Sax ton
Bottle ID
DW-21-1
DW-21-2
DW-21-3
DW-21-4
DW-21-5
DW-21-6
Date / Time
of Sample
5/14/09
3/14/09
8/14/09
8/14/09
J/14/09
8/14/09
Volume
Collected
125 mL
125 mL
125 mL
1 '^5 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
1IDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
overlying water tank
#1 control-Day 21
overlying water tank
#1 control-Day 21
overlying water tank
#1 125mg/L-Day 21
overlying water tank
#1 125mg/L-Day 21
overlying water tank
#1 500 mg/L-Day 21
overlying water tank
#1 500 mg/L-Day 21
Preservation
H2SO4
Cold
II,S04
Cold
I-I2SO4
Cold
Shipper:
L Ck.. "D a nc_
2.
Date/Time:
Receiver:
Date/Time:
Wo?
Temperature:
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
of
Lates Smrfronmartial Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.clec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
DW-21-7
DW-21-8
DW-21-9
DW-21-10
Date / Time
of Sample
8/14/09
8/14/09
8/14/09
5/14/09
Volume
Collected
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
hope
HOPE
HOPE
HOPE
Sample Description
(Site information/
sample type)
overlying water tank
#1 2000 mg/L-Day 21
overlying water tank
#1 2000 mg/L-Day 21
Stock solution 59.4
g/L NaNC)3 -30,Gg/L
Nitrate - Day 24
Stock solution 59.4
g/L NaN03 -30.0g/L
Nitrate - Day 24
Preservation
h2so4
Cold
HiSO.
Cold
Shipper:
(L ,
Date/Time:
Receiver:
2.
ir 9CJ1
Date/Time:
"5
Temperature:
Abbreviating for otliiT frequently minted jp.iKsts
NCh-N = Nitrite Nitrogen. Total N = Total Nitrogen
NO3 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus.
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page I of
GleC
Lates Environmental Center
739 Hastings St
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location: J
Date;
Contact Person:
Email Address:
Phone Number:
Collector:
Slnloi
Mailee Garton/Jamie Saxton
Bottle ID
DW-24-1
DW-24-2
DW-24-3
DW-24-4
DW-24-5
Date / Time
of Sample
8/17/09
8/17/09
8/17/09
8/17/09
8/17/09
Volume
Collected
125 mL
1'^5 LIll—'
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site Information /
sample type)
overlying water tank
#2 control-Day 24
overlying water tank
#2 control-Day 24
overlying water tank
#1 250mg/L-Day 24
overlying water tank
#1 250mg/L-Day 24
overlying water tank
#1 1000 mg/L-Day 24
Check the requested analysis
Preservation
h,so4
Cold
H2SO4
Cold
h2so4
DW-24-6
8/17/09
125 ml <
HDPE
overlying water tank
#1 1000 mg/L-Day 24
Cold
Shipper:
Date/Time:
i.uTfa.of, SnjM tiio
Receiver:
fh, dfi
Date/Time:
Temperature:
Abbreviations for other 11 cOUCJitH 1 wiuested analysts
NOj -N = Nitrite Nitrogen "" Total N = Total Nitiopen
NO3-N - Nitrate Nitrogen SRP= Soluble Reactive Phosphonit
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of ^
Greet Lates Environmental Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Sax ton
Bottle ID
DW-24-7
DW-24-
Date/Time
of Sample
8/17/09
8/17/09
Volume
Collected
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
Sample Description
(Site information /
sample type)
Stock solution 59.4
g/L NaN03 -301fg/L
Nitrate - Day 24
Stock solution 59.4
g/L NaN03 -3#^g/L
Nitrate - Day 24*f5' 5
Date/Time:
Shipper:
^(njo£i K3q
Receiver:
AM
Date/Time:
S -(7-o?
Temperature:
2.
^§§1,
NCtyNOj-N = Nitrate/Nitntc-Nitrogen
Abbreviations for-olhtr fiwmaillt roaiirsted jimlvses
NOj -N = Nitrite Nitrogen. " Total N = Total Nitrogen
NOi -N = Nitfile Nitrogen SRF = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
of
GleC
Greal Lakss Environmental Cenlef
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
-id-
Bottle ID
DW-26-1
DW-26-2
DW-26-3
DW-26-4
DW-26-5
DW-26-6
Date / Time
of Sample
8/19/09
8/19/09
8/19/09
8/19/09
8/19/09
8/19/09
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
overlying water tank
#3 control-Day 26
overlying water tank
#3 control-Day 26
overlying water tank
#2 125mg/L-Day 26
overlying water tank
#2 125mg/L-Day 26
overlying water tank
#2 500 mg/L-Day 26
overlying water tank
#2 500 mg/L-Day 26
Date/Time:
Shipper:
1.8?'*1 1°
2.
Receiver:
xJ
BODj = Biojhenw al lAyfren Demnd S m*
NH3-N = Ammonia-Nitrogen
N03/N02-N = Nilrate/Nitnte-Nitrogcn
I>efirtlt»niis
- 1 oLil Di-sr l\e Sl-IuL
'J'KN = Total KjeldaM Nmogcn
Date/Time:
Temperature:
TP = Total Phosphorus
TSS = Total Suspended Solids
.""AMwgviatimis for oIIut fn-oucntlv requested analysts
NO?-N = Nitrite-Nitrogen- Tota1N1= Toial/Nitrogen
NO, -N = Nitrate Nitrogen SRF' = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page,
of
GleC
GreaT Latos Environ menial Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mai lee Garton/Jamie Sax ton
Bottle ID
DW-26-7
DW-26-
Date / Time
of Sample
8/19/09
8/19/09
Volume
Collected
125 mL
125 mi.
Type of
Sample
Container
HDPE
HDPE
Sample Description
(Site information /
sample type)
overlying water tank
#2 2000 mg/L-Day 26
overlying water tank
#2 2000 mg/L-Day 26
Check the requested analysis (see definitions below)
Preservation
H?SO,
h2so4
Date/Time:
Shipper:
Receiver:
2.
Date/Time:
Temperature:
, i0B3glI®fenicaI Oxv^'peiii5nft~Sraay
NH3-N = Ammonia-Nitrogen.,
NQ3/NO1-N = Nrtiate/Nitntse-Nitrogen -
TKN =-Total KjekJah] Nitrogen." TSS = Total Suspended Solids
Alihi-fviatinn.s fOT'othcrfrwrncntly rcaiifsted'analvses
NOj-N = Nitrite Nitrogen Total N = Total Nitrogen
NOj-N = Nitrate Nitrogen SKP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
of
GleC
Great Lakes Environmental Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www-glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
DW-28-1
DW-28-2
DW-28-3
DW-28-4
DW-28-5
DW-28-6
Date / Time
of Sample
8/21/09
mum
5/21/09
8/21/09
5/21/09
8/21/09
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
overlying water tank
#4 control-Day 28
overlying water tank
#4 control-Day 28
overlying water tank
#2 250rng/L-Day 28
overlying water tank
#2 250mg/L-Day 28
overlying water tank
#2 1000 mg/L-Day 28
overlying water tank
#2 1000 mg/L-Day 28
H2SO,
Shipper:
Date/Time:
Receiver:
&
Date/Time:
T-2I-Q1
Temperature:
m
m Definitions
BODj = Biochemical Oxygen Demand-5 day IDS = Total IJissolve Solids
NI l-i -N = Ammonia-Nitrogen TKN = Total Kjcldahl Nitrogen
NO3/NO2.-N = NUrate/Nitriti'-Nitrogen
-TP - Total Phosphorus.
TSS = 'Total Suspended Solids
, AbbrcViatjunsfor otherfrcoiientl v rttiuosirdaiialvses
NO2 -N = Nitrite Nitrogen
NOj -N = Nitrate Nitrogen
Total N"= Tolal Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of
739 Hastings St,
Traverse City, MI 49686
Phone; 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description (Site
information / sample
type)
Preservation
Check the requested analysis (see definitions below1
. 15
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a
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Nit-rate .
DW-32-1
8/25/09
125 ml.
HDPE
overlying water tank #1
control Nitrate -Day 32
h2so4
X'"V
DW-32-2
8/25/09
125 mL
HOPE
overlying water tank #2
control Nitrate -Day 32
h2so4
X
DW-32-3
8/25/09
125 mL
HDPE
overlying water tank #3
control Nitrate -Day 32
h2so4
x. :
DW-32-4
8/25/09
125 mL
HDPE
overlying water tank #4
control Nitrate -Day 32
h2so4
X
DW-32-5
8/25/09
125 mL
HDPE
overlying water tank #1
125 mg/L Nitrate-Day 32
h2so4
ft: v
X
DW-32-6
8/25/09
125 mL
HDPE
overlying water tank #2
125 mg/L Nitrate -Day 32
H1S04
X
Date/Time:
Receiver:
A. drf
Date/Time:
Temperature:
BODj = Biochemical Oxygen Demand-S day
NHj -N = Ammonia-Nitrogen
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS - Total Dissolve Solids TP = Total Phosphorus
TKN = Total Kjeldahl "Nitrogen TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NO, -N = Nitrite Nitrogen Total N = Total Nitrogen
NO3 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY
(To be completed and submitted
Gl
RECORD
with samples)
Page
of
I,«rve-c,
739 Hastings St,
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description (Site
information / sample
type)
Preservation
Check the requested analysis {see definitions below)
¦s3>-
<
O
O
m
•'!S-
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0
. ...Jm
Chlorophyll a
SO
.oe
•• e
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z
z
- .1*
•roi
O
-'Z
-
O
Z
w
Q
H
H
c-
H
m ¦
m
H
Nitrate
DW-32-7
8/25/09
125 ml.
HOPE
overlying water tank #3 125
mg/L Nitrate -Day 32
h2so4
x .
DW-32-8
8/25/09
125 mL
HDPE
overlying water tank #4 125
mg/L Nitrate -Day 32
h2so4
X .
DW-32-9
8/25/09
125 mL
HDPE
overlying water tank #1 250
mg/L Nitrate -Day 32
h,so4
X
DW-32-10
8/25/09
125 mL
HDPE
overlying water tank #2 250
mg/L Nitrate -Day 32
H2so4
X «
DW-32-11
8/25/09
125 mL
HDPE
overlying water tank #3 250
mg/L Nitrate -Day 32
h2so,
X' ;
DW-32-12
8/25/09
125 mL
HDPE
overlying water tank #4 250
mg/L Nitrate -Day 32
HjS04
x ;
Shipper:
Date/Time:
Receiver:
£
Date/Time:
* -7S-Q1
Temperature:
BOD* = Biochemical Oxygen Demand-5 day
NH5 -N = Ammonia-Nitrogen
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN - Total Kjeldahl Nitrogen
TP " Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NO2-N = Nitrite Nitrogen Total N = Total Nitrogen
NO3 -N = Nitrate Nitrogen SRP =¦ Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
739 Hastings St.
Traverse Citv, Ml 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Great Lakes Environmental Center
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description (Site
information / sample
type)
Preservation
Check the requested analysis (see definitions below)
(
O
O
aa
j Chloride
Chlorophyll a
1
1
p*
as
z
" 'Z.
.1
' M
O
M ¦
g
CO
0
H
H
eu
H
.•co-
co
.-(J)-.:
-
DW-32-13
8/25/09
125 mL
HOPE
overlying water tank #1 500
mg/L Nitrate -Day 32
H;S04
= -
- ¦
X-
DW-32-14
8/25/09
125 mL
HDPE
overlying water tank #2 500
mg/L Nitrate -Day 32
h2so4
r-.
r'- ' ' .
%b;
DW-32-15
8/25/09
125 mL
HDPE
overlying water tank #3 500
mg/L Nitrate -Day 32
H2S04
X '
DW-32-16
8/25/09
125 mL
HDPE
overlying water tank #4 500
mg/L Nitrate -Day 32
H2S04
: *•; •
X .
DW-32-17
8/25/09
125 mL
HDPE
overlying water tank #1
1000 mg/L Nitrate -Day 32
H2S04
X'".'/-
DW-32-18
8/25/09
125 mL
HDPE
overlying water tank #2
1000 mg/L Nitrate -Day 32
h2so4
X
Shipper:
Date/Time:
Receiver:
1)>«, cJ
Daie/Time:
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NH;i -N = Ammonia-Nitrogen
NOs/NOi -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS - Total Dissolve Solids TP = Total Phosphorus
TKN = Total Kjeldahl Nitrogen TSS - Total Suspended Solids
Abbreviations for other frequently requested analyses
NOi -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
739 Hastings St
Traverse City, MI 49686
Phone: 231-941-2230
Fax:231-941-2240
www.giee.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
DW-32-19
Date / Time
of Sample
8/25/09
Volume
Collected
125 mL
Type of
Sample
Container
HDPE
Sample Description (Site
information / sample type)
overlying water tank #3 1000
mg/L Nitrate -Day 32
Preservation
H2S04
.
-:*2-
a
o
m
Cheek the requested analysis (see definitions below)
;"T3'
¦C
u
J5
&
o
•*C\'
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<39 .
X
X
z
o
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o
z
w
o
0,
:W
ta
H
e#-
tm
DW-32-20
8/25/09
125 mL
HDPE
overlying water tank #4 1000
mg/L Nitrate -Day 32
H2SO4
X
Shipper:
Date/Time:
Receiver:
Ik OC/f
Date/Time:
S -IS'O^
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NHi -N = Ammonia-Nitrogen
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS " Total Dissolve Solids TP = Total Phosphorus
TKN = Total Kjeidahl Nitrogen TSS = Total Suspended Solids
Abbreviations for other frequently requested analysts
N02 -N = Nitrite Nitrogen Total N - Total Nitrogen
NOs -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of
J' r '! — /
a ~f i. $
V_J i...i ..
Trawse'ClfyTW^^SS
Phone: 231-941-2230
Fax: 231-941-2240
vvww.glec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ED
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below"
- IE-'-
.--"a.i,
a
0
m
Chloride
Chlorophyll a
Hardness
z
X
z
i-M -
-»*V
0
z
TDS
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X
H
a.
H
SO
£0
H
»
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u
SS-'
% *
DW-32-25
8/25/09
125 rnL
HOPE
Stock solution 59.4
g/L NaN03 -43.3g/L
Nitrate
h2so4
<
X -
X
X
X •.
X
Shipper:
Date/Time:
Receiver:
tX, DU
Date/Time:
S-2 ycz
Temperature:
Definitions
BOD5 = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj-N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NO3/NO2 -N - Nitrate/Nitrite-Nitrogen
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
N03 -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY
(To be completed and submitted
DTfrrtBTI
with samples)
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone; 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
r . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description (Site
information / sample
type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity . j
V3
Q
O
oa
Chloride
Chlorophyll a
s " /
z.
ffl
¦
r4
O .
' O
. Z
CO
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H
m
m '
H
DW-32-26
8/25/09
125 mL
HDPE
overlying water tank #1
control Nitrate -Day 32
COLD
X':7
j--
DW-32-27
8/25/09
125 mL
HDPE
overlying water tank #2
control Nitrate -Day 32
COLD
!r;
DW-32-28
8/25/09
125 mL
HDPE
overlying water tank #3
control Nitrate -Day 32
COLD
r;
x-'~-
DW-32-29
8/25/09
125 mL
HDPE
overlying water tank #4
control Nitrate -Day 32
COLD
"
*
x '
DW-32-30
8/25/09
125 mL
HDPE
overlying water tank #1
125 mg/L Nitrate -Day 32
COLD
V-: .1- 'V*
X :
DW-32-31
8/25/09
125 mL
HDPE
overlying water tank #2
125 mg/L Nitrate -Day 32
COLD
:v
? .
;•' •
X
Shipper:
Date/Time:
Receiver:
Date/Time:
•g--2S-o
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax:231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Gorton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description (Site
information / sample
type)
Preservation
Check the requested analysis (see definitions below
£
'.5.
'13'
<
a
0
. ; ^ - -
•
'-"'IE, '
. y ;
Chlorophyll a
t/i-
m-:
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a
z
X
z
z
0
z
m
a
s.
f-*
CO
CO
' '
' p..
DW-32-32
8/25/09
125 mL
IIDPE
overlying water tank #3
125 mg/L Nitrate -Day 32
COLD
X '
DW-32-33
8/25/09
125 mL
HDPE
overlying water tank #4
125 mg/L Nitrate -Day 32
COLD
X
DW-32-34
8/25/09
125 mL
HOPE
overlying water tank #1
250 mg/L Nitrate -Day 32
COLD
;-
x
DW-32-35
8/25/09
125 mL
HDPE
overlying water tank #2
250 mg/L Nitrate -Day 32
COLD
X
DW-32-36
8/25/09
125 mL
HDPE
overlying water tank #3
250 mg/L Nitrate -Day 32
COLD
-
Zp'-'-y '!
DW-32-37
8/25/09
125 mL
HDPE
overlying water tank #4
250 mg/L Nitrate -Day 32
COLD
w- •
Shipper:
Date/Time:
jZjXjcTi
Receiver:
&j> c(
Date/Time:
I'75-of
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NH3 -N -¦ Ammonia-Nitrogen
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen
¦ Definitions
I DS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
N02 -N = Nitrite Nitrogen Total N - Total Nitrogen
NOs -N — Nitrate Nitrogen SRP — Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
739 Hastings St,
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
, Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
of
Mailee Garton/Jamie Saxion
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description (Site
information / sample
type)
Preservation
Cheek the requested analysis (see definitions below]
' &
-re;:,.:
ilsr ..
.2d .
5"
un
a
0
m
' - o>: /.
'.. ;
"V©;; •
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u
Chlorophyll a
Hardness
Z
as
z.
¦ z,
-•»: --
r «S1 '
. O '
:S5
CJ
0
z
TDS
-H* '
fi.
H
". w-
: - - 00
H
• Nitrite
DW-32-38
8/25/09
125 ml.
HOPE
overlying water tank #1
500 mg.'I. Nitrate -Day 32
COLD
X
DW-32-39
B/25/09
125 ml.
HDPE
overlying water tank #2
500 mg/L Nitrate -Day 32
COLD
X
DW-32-40
8/25/09
125 mL
HOPE
overlying water tank #3
500 mg/L Nitrate -Day 32
COLD
X
DW-32-41
8/25/09
125 mL
HDPE
overlying water tank #4
500 mg/L Nitrate -Day 32
COLD
X
DW-32-42
8/25/09
125 mL
HDPE
overlying water tank #1
1000 mg/L Nitrate -Day
32
COLD
X
DW-32-43
8/25/09
125 mL
HDPE
overlying water tank #2
1000 mg/L Nitrate -Day
32
COLD
X
Shipper:
Date/Time:
Receiver:
Date/Time:
S-25-of
Temperature:
BOD, = Biochemical Oxygen Demsuid-S day
NHj -N = Ammonia-Nitrogen
NOj/NO; -N = N itraie/Nitrite-N itrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS= Total Suspended Solids
Abbreviations for othtr frequently requested analyses
NOz-N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.giec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description (Site
information / sample
type)
Preservation
>%¦
;-C::
£3
O
«
Check the requested analysis (see definitions below)
JTjjjjf;
"w
JD
3*
U
c-
o
U
a
¦*0
Z
o
¦Z-
¦
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of
TfaverslSyrRT'll'®
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garten/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
a
o
Chloride
Chlorophyll a
•' "fas
*c«
; Je
. «
-w
Z
X
z
z
o
;:z •
o"
z
TDS
- H
a#
&
H
' w
¦. 'ST'
DW-32-50
8/25/09
125 ml.
HOPE
Stock solution 59.4
g/L NaN03 -43.3g/L
Nitrate
COLD
X '
:£'.
X ;
-
X '
"r--
-
X.
•
i- .
X
-
Shipper:
Date/Time:
Receiver:
Date/Time:
8 - 7S -Cf\
Temperature:
BOD5 = Biochemical Oxygen Demand-5 day
NH] -N = Ammonia-Nitrogen
Definitions
TDS - Total Dissolve Solids TP = Total Phosphorus
TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj-N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
Date
Dilution Water ControlRep 1
Test Day Temperature pH DO
24/07/2009
0
24.8
8,05
7.5
25/07/2009
1
24.1
7.7
26/07/2009
2
25.7
7.5
27/07/2009
3
24.3
8.23
7,3
28/07/2009
4
29/07/2009
5
26.0
7,49
7.4
30/07/2009
6
25.1
31/07/2009
7
01/08/2009
8
02/08/2009
9
03/08/2009
10
25,3
7.60
7.4
04/08/2009
11
05/08/2009
12
25.0
8.26
7.3
06/08/2009
13
07/08/2009
14
08/08/2009
15
25,1
09/08/2009
16
25.2
10/08/2009
17
11/08/2009
18
26.3
12/08/2009
19
25.3
8.27
7,8
13/08/2009
20
24.0
14/08/2009
21
15/08/2009
22
16/08/2009
23
25.8
17/08/2009
24
25.2
8.01
7,2
18/08/2009
25
19/08/2009
26
20/08/2009
27
26.2
21/08/2009
28
25.6
8.10
7,2
22/08/2009
29
23/08/2009
30
25.7
24/08/2009
31
25/08/2009
32
25.3
7.73
7,6
SC Hardness Alkallinity Ammonia
298.0 136.0 96.0 ND
306.6
ND
293.0
ND
300.0
ND
293.0
224.0
90.0 ND
N
19
9
11
5
2
2
Max
26.3
8.27
7.8
306,6
224.0
96.0
Min
24.0
7.49
7.2
293.0
136.0
90.0
Mean
25.3
7,97
7.4
298.1
180.0
93.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
Dilution Water ControlRep 2
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
24/07/2009
0
24.8
8.17
7.8
298.0
25/07/2009
1
24.2
7.8
26/07/2009
2
25,1
7,5
27/07/2009
3
28/07/2009
4
24.6
29/07/2009
5
30/07/2009
6
31/07/2009
7
25,0
7.85
7.6
01/08/2009
8
24.4
02/08/2009
9
03/08/2009
10
24,6
8.28
7.4
296.0
04/08/2009
11
25.4
05/08/2009
12
06/08/2009
13
07/08/2009
14
24,7
8.16
8.2
08/08/2009
15
24,7
09/08/2009
16
10/08/2009
17
11/08/2009
18
26.0
12/08/2009
19
25 3
8.24
7.9
13/08/2009
20
24.6
14/08/2009
21
15/08/2009
22
16/08/2009
23
25.7
17/08/2009
24
25.4
8.05
7.2
305.0
18/08/2009
25
19/08/2009
26
25.4
8.48
7,9
20/08/2009
27
21/08/2009
28
25,3
8.13
7.3
22/08/2009
29
24.7
23/08/2009
30
25,1
24/08/2009
31
24.5
25/08/2009
32
25.0
7.77
7,5
283.0
N
21
9
11
4
Max
26.0
8 48
8,2
305.0
Min
24.2
7.77
7.2
283.0
Mean
25.0
8.13
7.6
295.5
ND
ND
ND
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
Dilution Water ControlRep 3
Date Test Day Temperature pH DO SC Hardness Aikallinity Ammonia
24/07/2009
0
24.7
8,23
7.9
25/07/2009
1
24.1
7.8
26/07/2009
2
25.1
7.5
27/07/2009
3
24.7
8,16
7.3
309.8
28/07/2009
4
29/07/2009
5
25.6
8.29
7.4
30/07/2009
6
31/07/2009
7
25.0
8.24
7.6
01/08/2009
8
02/08/2009
9
25.4
03/08/2009
10
04/08/2009
11
25.5
05/08/2009
12
06/08/2009
13
24.6
07/08/2009
14
24.7
8.20
8.0
08/08/2009
15
09/08/2009
16
10/08/2009
17
24.5
8.27
7.7
281.0
11/08/2009
18
12/08/2009
19
25.4
13/08/2009
20
14/08/2009
21
25.2
8.11
8.2
15/08/2009
22
25.1
16/08/2009
23
17/08/2009
24
25.4
18/08/2009
25
25.6
19/08/2009
26
20/08/2009
27
25,5
21/08/2009
28
22/08/2009
29
24.5
23/08/2009
30
24/08/2009
31
24,4
25/08/2009
32
24.8
7.84
7.7
N
20
8
10
2
Max
25.6
8.29
8.2
309.8
Min
24.1
7.84
7.3
281.0
Mean
25,0
8.17
7.7
295.4
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
Dilution Water ControlRep 4
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
24/07/2009
0
24.7
8.14
8.0
25/07/2009
1
24.2
7.8
26/07/2009
2
25.2
7.4
27/07/2009
3
28/07/2009
4
24,3
29/07/2009
5
30/07/2009
6
24.7
31/07/2009
7
01/08/2009
8
24.4
02/08/2009
9
25.6
03/08/2009
10
04/08/2009
11
05/08/2009
12
24.8
8.36
7.2
06/08/2009
13
24.8
07/08/2009
14
08/08/2009
15
09/08/2009
16
24.6
10/08/2009
17
24.7
8.28
7.8
296.0
11/08/2009
18
12/08/2009
19
25.4
13/08/2009
20
14/08/2009
21
25.5
8.20
8.1
15/08/2009
22
25.5
16/08/2009
23
17/08/2009
24
25.4
18/08/2009
25
26.1
19/08/2009
26
25.7
8.58
7.7
20/08/2009
27
21/08/2009
28
22/08/2009
29
24.5
23/08/2009
30
24/08/2009
31
25/08/2009
32
25.3
7.89
7,5
N
19
6
8
1
Max
26.1
8.58
8.1
296.0
Min
24.2
7,89
7.2
296.0
Mean
25.0
8.24
7.7
296.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD £3-12 Nitrate
Pimephales promelas (Fathead Minnow)
125 mg/L Nitrate Rep 1
Date Test Day Temperature pH DO SC Hardness Alkaliinity Ammonia
24/07/2009 0 24,8 8.25 7.8 391,0 136.0 104,0 ND
25/07/2009 1 24.2 7.8
26/07/2009 2 25.0 7.4
27/07/2009 3 24,4 8.26 7.5 446.8 ND
28/07/2009 4
29/07/2009 5 25.7 8.61 7,5
30/07/2009 6 24.7
31/07/2009 7
01/08/2009 8
02/08/2009 9
03/08/2009 10 24.6 8.34 7.9 348.0 ND
04/08/2009 11
05/08/2009 12
06/08/2009 13
07/08/2009 14
08/08/2009 15
09/08/2009 16
10/08/2009 17
11/08/2009 18 25.8
12/08/2009 19 25.4 8,24 8.0
13/08/2009 20 24.0
14/08/2009 21
15/08/2009 22
16/08/2009 23 24,9
17/08/2009
24
24.7
8,08
7.2
487.0
ND
18/08/2009
25
19/08/2009
26
20/08/2009
27
25.4
21/08/2009
28
24.3
7.79
8.4
22/08/2009
29
23/08/2009
30
25.1
24/08/2009
31
25/08/2009
32
24,8
8.09
6.7
539.0
172.0
104.0 ND
N
16
8
10
5
2
2
Max
25.8
8.61
8.4
539.0
172.0
104.0
Min
24.0
7.79
6.7
348.0
136.0
104.0
Mean
24.9
8.21
7.6
442,4
154.0
104.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
125 mg/L Nitrate Rep 2
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
24/07/2009 0 24,9 8.16 7.8 392.0
25/07/2009 1 24.1 7.8
26/07/2009
2
25,0
7.1
27/07/2009
3
28/07/2009
4
24.6
29/07/2009
5
30/07/2009
6
31/07/2009
7
24.8
8.24
7,6
01/08/2009 .
8
24.4
02/08/2009
9
03/08/2009
10
24.6
8.35
8.1
374.0
04/08/2009
11
25.4
05/08/2009
12
25.0
8.41
7,4
06/08/2009
13
07/08/2009
14
24.7
8.23
8.2
08/08/2009
15
24.4
09/08/2009
16
24,7
10/08/2009
17
11/08/2009
18
26.0
12/08/2009
19
25.4
8.24
8.0
13/08/2009
20
24.4
14/08/2009
21
15/08/2009
22
16/08/2009
23
25.5
17/08/2009
24
25.0
8.09
7.4
506,0
18/08/2009
25
19/08/2009
26
25.4
8.56
7,7
20/08/2009
27
21/08/2009
28
24,8
8,11
7.5
22/08/2009
29
23/08/2009
30
25.8
24/08/2009
31
24.6
25/08/2009
32
25.0
7.84
7.4
658.0
N
22
10
12
4
Max
26.0
8.56
8,2
658.0
Min
24.1
7.84
7.1
374.0
Mean
24,9
8.22
7.7
482,5
ND
ND
ND
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECDB-12 Nitrate
Pimephales promelas (Fathead Minnow)
125 mg/L Nitrate Rep 3
Date Test Day Temperature pH DO SC Hardness AikaMinity Ammonia
445.2
24/07/2009
0
25.1
8.21
7.8
25/07/2009
1
24.2
7,8
26/07/2009
2
25.1
7.3
27/07/2009
3
24.8
8.18
7,4
28/07/2009
4
29/07/2009
5
25.7
8.41
7.6
30/07/2009
6
31/07/2009
7
24.8
8.31
7,7
01/08/2009
8
02/08/2009
9
25.4
03/08/2009
10
04/08/2009
11
25.6
05/08/2009
12
25.1
8.34
7.8
06/08/2009
13
24.7
07/08/2009
14
24.7
8.27
7.8
08/08/2009
15
24.6
09/08/2009
16
24.8
10/08/2009
17
24.6
8.28
7.8
11/08/2009
18
12/08/2009
19
25.4
13/08/2009
20
14/08/2009
21
25.3
8.23
9,2
15/08/2009
22
25.4
16/08/2009
23
17/08/2009
24
25.0
18/08/2009
25
25.7
19/08/2009
26
20/08/2009
27
25.7
21/08/2009
28
22/08/2009
29
24.7
23/08/2009
30
24/08/2009
31
24.5
25/08/2009
32
25.0
8.17
7.4
407.0 ND
N
23
9
11
2
Max
25.7
8.41
9,2
445.2
Mill
24.2
8.17
7,3
407.0
Mean
25.0
8.27
7.8
426.1
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12. Nitrate
Pimephales promelas (Fathead Minnow)
125 mg/L Nitrate Rep 4
Date Test Day Temperature pH DO SC Hardness Alkaliinity Ammonia
24/07/2009 0 25.1 8.24 7.9
25/07/2009 1 24.2 7.8
26/07/2009 2 25.4 7.3
27/07/2009 3
28/07/2009 4 24,4
29/07/2009 5
30/07/2009 6 24.9
31/07/2009 7
01/08/2009 8 24.6
02/08/2009 9 25.7
03/08/2009 10
04/08/2009 11
05/08/2009 12
06/08/2009 13 24.9
07/08/2009 14
08/08/2009 15
25.2
ND
09/08/2009
16
25.2
10/08/2009
17
24.7
8.29
7.8
418.0
11/08/2009
18
12/08/2009
19
25.3
13/08/2009
20
14/08/2009
21
25.4
8.26
9,2
15/08/2009
22
25.5
16/08/2009
23
17/08/2009
24
25.3
18/08/2009
25
25.7
19/08/2009
26
25.6
8,60
8.1
20/08/2009
27
21/08/2009
28
22/08/2009
29
24.7
23/08/2009
30
24/08/2009
31
25/08/2009
32
25.1
8.19
7.2
N
18
5
7
1
Max
25.7
8.60
9.2
418.0
Min
24.2
8.19
7.2
418.0
Mean
25.1
8,32
7.9
418.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
250 mg/L Nitrate Rep 1
Date Test Day
Temperature
pH DO
SC
Hardness
Alkallinity Ammonia
24/07/2009
0
25.1
8.31
7.8
625.0
128.0
102.0
25/07/2009
1
24.2
7.8
26/07/2009
2
25.5
7.4
27/07/2009
3
24.3
8.25
7.4
637 0
ND
28/07/2009
4
29/07/2009
5
26.0
8.79
7.5
30/07/2009
6
24.9
31/07/2009
7
01/08/2009
8
02/08/2009
9
03/08/2009
10
24.7
8.31
8.0
536.0
ND
04/08/2009
11
05/08/2009
12
24.9
8.34
7,3
06/08/2009
13
07/08/2009
14
08/08/2009
15
09/08/2009
16
10/08/2009
17
11/08/2009
18
26.0
12/08/2009
19
25,5
8.25
8.0
13/08/2009
20
14/08/2009
21
25.0
8.22
9.1
15/08/2009
22
16/08/2009
23
25.0
17/08/2009
24
24,7
8.06
7.4
668.0
ND
18/08/2009
25
19/08/2009
26
20/08/2009
27
25,4
21/08/2009
28
24.5
8,10
7.4
22/08/2009
29
23/08/2009
30
24/08/2009
31
25/08/2009
32
24.4
7.63
6.6
663.0
152.0
112.0 ND
N
16
10
12
5
2
2
Max
26.0
8.79
9.1
668.0
152.0
112.0
Min
24.2
7.63
6.6
536.0
128.0
102.0
Mean
25.0
8.23
7.6
625.8
140.0
107.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
Date
250 mg/L Nitrate Rep 2
Test Day Temperature pH
DO SC
Hardness Alkallinity
24/07/2009
0
24.8
8.20
7.8
626.0
25/07/2009
1
24.2
7.7.
26/07/2009
2
25.0
7,4
27/07/2009
3
28/07/2009
4
24.1
29/07/2009
5
30/07/2009
6
31/07/2009
7
24.8
8.27
7.6
01/08/2009
8
24.3
02/08/2009
9
03/08/2009
10
24,7
8.33
8.0
559,0
04/08/2009
11
25.4
05/08/2009
12
24.8
8.37
7.4
06/08/2009
13
07/08/2009
14
24.7
8.20
8,1
08/08/2009
15
24.7
09/08/2009
16
24.6
10/08/2009
17
11/08/2009
18
25.8
12/08/2009
19
25.4
8.24
8.0
13/08/2009
20
14/08/2009
21
15/08/2009
22
16/08/2009
23
25.4
17/08/2009
24
25.3
8.07
7.4
699.0
18/08/2009
25
19/08/2009
26
25.4
8.60
8.1
20/08/2009
27
21/08/2009
28
24.5
8.18
7.6
22/08/2009
29
23/08/2009
30
24.8
24/08/2009
31
24.6
25/08/2009
32
25.1
7.71
7.9
872.0
N
21
10
12
4
Max
25 8
8.60
8.1
872.0
Min
24.1
7.71
7.4
559,0
Mean
24.9
8.22
7.8
689.0
Ammonia
ND
ND
ND
ND
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
250 mg/L Nitrate Rep 3
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
632.0
24/07/2009
0
24.8
8.11
7.8
25/07/2009
1
24.3
7,7
26/07/2009
2
25.3
7.3
27/07/2009
3
25.1
8.18
7.4
28/07/2009
4
29/07/2009
5
26.0
8,38
7.4
30/07/2009
6
31/07/2009
7
24.9
8.29
7.7
01/08/2009
8
02/08/2009
9
25.7
03/08/2009
10
04/08/2009
11
25.8
05/08/2009
12
06/08/2009
13
24,9
07/08/2009
14
24.6
8.19
8.0
08/08/2009
15
24.7
09/08/2009
16
25.1
10/08/2009
17
24.7
8.28
7.9
11/08/2009
18
12/08/2009
19
25.4
13/08/2009
20
24.4
14/08/2009
21
15/08/2009
22
25.7
16/08/2009
23
17/08/2009
24
25.4
18/08/2009
25
26.1
19/08/2009
26
20/08/2009
27
25.9
21/08/2009
28
22/08/2009
29
24.5
23/08/2009
30
25.1
24/08/2009
31
24,7
25/08/2009
32
25.1
7,70
7.1
676.0 ND
N
23
7
9
2
Max
26.1
8.38
8.0
676,0
Min
24.3
7.70
7,1
632,0
Mean
25,1
8.16
7.6
654.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephaies promelas (Fathead Minnow)
250 mg/L Nitrate Rep 4
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
24/07/2009
0
24,9
8.24
7.8
25/07/2009
1
24.2
7.8
26/07/2009
2
25.5
7,3
27/07/2009
3
28/07/2009
4
24.6
29/07/2009
5
30/07/2009
6
24.9
31/07/2009
7
01/08/2009
8
24.6
02/08/2009
9
25.7
03/08/2009
10
04/08/2009
11
05/08/2009
12
06/08/2009
13
25.0
07/08/2009
14
08/08/2009
15
09/08/2009
16
25.2
10/08/2009
17
24.8
8.27
7.8
667.0
11/08/2009
18
12/08/2009
19
25.6
13/08/2009
20
24.4
14/08/2009
21
25.5
8.26
9.0
15/08/2009
22
25.6
16/08/2009
23
17/08/2009
24
25.3
18/08/2009
25
26,0
19/08/2009
26
25.9
8.52
7.9
20/08/2009
27
21/08/2009
28
22/08/2009
29
24.5
23/08/2009
30
24/08/2009
31
25/08/2009
32
25,2
7.86
7.0
N
19
5
7
1
Max
26.0
8,52
9.0
667.0
Min
24.2
7.86
7.0
667.0
Mean
25.1
8.23
7.8
667.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
500 mg/L Nitrate Rep 1
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
24/07/2009 0 24.6 8,22 7.6 1070.0 192,0 102.0 ND
25/07/2009 1 24.2 7,8
26/07/2009 2 24.8 7.3
27/07/2009 3 24.0 8.25 7,4 1024.0 ND
28/07/2009 4
29/07/2009 5 25.7 8.75 7.5
30/07/2009 6 24.6
31/07/2009 7
01/08/2009 8
02/08/2009 9
03/08/2009 10 24.3 8.35 8.2 812.0 ND
04/08/2009 11
05/08/2009 12 25.1 8.37 7.8
06/08/2009 13
07/08/2009 14
08/08/2009 15 24.3
09/08/2009 16 24.7
10/08/2009 17
11/08/2009 18 26.0
12/08/2009 19 25.2 8,26 8.0
13/08/2009 20 24.0
14/08/2009 21
15/08/2009 22
16/08/2009 23 24.9
17/08/2009 24 25.0 8.04 7.7 1094.0 ND
18/08/2009 25
19/08/2009 26
20/08/2009 27 25.5
21/08/2009 28 24.0 8.07 7.3
22/08/2009 29
23/08/2009 30 24.7
24/08/2009 31
25/08/2009 32 24.8 8.10 6.8 1227.0 136,0 100.0 ND
N
19
9
11
5
2
2
Max
26.0
8.75
8.2
1227,0
192.0
102.0
Min
24.0
8.04
6,8
812.0
136.0
100.0
Mean
24.8
8.27
7.6
1045,4
164.0
101.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
500 rng/L Nitrate Rep 2
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
24/07/2009 0 24.7 8.16 7.7 1070.0
25/07/2009 1 24.1 7.7
26/07/2009 2 24.9 7,5
27/07/2009 3
28/07/2009 4 24.6
29/07/2009 5
30/07/2009 6
31/07/2009 7 24.7 8.29 7.5
01/08/2009 8 24.3
02/08/2009 9
03/08/2009 10 24,4 8.35 8.3 813.0 ND
04/08/2009 11 25,4
05/08/2009 12
06/08/2009 13
07/08/2009 14 24.3 8.19 8.2
08/08/2009 15 24.4
09/08/2009 16 24.6
10/08/2009 17
11/08/2009 18 26.0
12/08/2009 19 25.2 8.27 8.0
13/08/2009 20 24.0
14/08/2009 21
15/08/2009 22
16/08/2009 23 25.0
17/08/2009 24 24.5 8.04 7,7 1104,0 ND
18/08/2009 25
19/08/2009 26 25.1 8.57 7.6
20/08/2009 27
21/08/2009 28 23.7 8,12 7.5
22/08/2009 29
23/08/2009 30 24.6
24/08/2009 31 24.3
25/08/2009 32 24.8 8.16 7,0 1345.0 ND
N
21
9
11
4
Max
26.0
8.57
8.3
1345.0
Min
23.7
8.04
7.0
813.0
Mean
24.6
8.24
7,7
1083.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
500 mg/L Nitrate Rep 3
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
1014.0
24/07/2009
0
24.7
8.21
7.7
25/07/2009
1
24.2
7,7
26/07/2009
2
24.9
7.4
27/07/2009
3
24.7
8.16
7.4
28/07/2009
4
29/07/2009
5
25.6
8.48
7.5
30/07/2009
6
31/07/2009
7
24.7
8.29
7.7
01/08/2009
8
02/08/2009
9
25.4
03/08/2009
10
04/08/2009
11
25.4
05/08/2009
12
06/08/2009
13
24.6
07/08/2009
14
24.3
8.20
8.1
08/08/2009
15
09/08/2009
16
24.6
10/08/2009
17
24.6
8.27
8.0
11/08/2009
18
12/08/2009
19
25.4
13/08/2009
20
24.9
14/08/2009
21
25.1
8.22
9,4
15/08/2009
22
24.3
16/08/2009
23
17/08/2009
24
24.6
18/08/2009
25
25.4
19/08/2009
26
20/08/2009
27
25.6
21/08/2009
28
22/08/2009
29
24.8
23/08/2009
30
24/08/2009
31
24.4
25/08/2009
32
25.0
8,21
7.6
954,0 ND
N
22
8
10
2
Max
25,6
8.48
9,4
1014.0
Min
24.2
8.16
7.4
954.0
Mean
24.9
8.26
7.9
984,0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
500 mg/L Nitrate Rep 4
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
24/07/2009
0
25.2
8.15
7.8
25/07/2009
1
24.2
7.7
26/07/2009
2
24.8
7.4
27/07/2009
3
28/07/2009
4
24.6
29/07/2009
5
30/07/2009
6
24.4
31/07/2009
7
01/08/2009
8
23.7
02/08/2009
9
25.4
03/08/2009
10
04/08/2009
11
05/08/2009
12
24.9
8.34
7.6
06/08/2009
13
24.6
07/08/2009
14
08/08/2009
15
09/08/2009
16
10/08/2009
17
24.6
8.27
8.0
958.0
11/08/2009
18
12/08/2009
19
25.2
13/08/2009
20
24.9
14/08/2009
21
25.2
8.19
9.3
15/08/2009
22
24.4
16/08/2009
23
17/08/2009
24
25.1
18/08/2009
25
25.5
19/08/2009
26
25.2
8.39
7,6
20/08/2009
27
21/08/2009
28
22/08/2009
29
24.5
23/08/2009
30
24/08/2009
31
25/08/2009
32
24.7
8.24
7.1
N
19
6
8
1
Max
25.5
8.39
9.3
958.0
Min
23,7
8.15
7,1
958.0
Mean
24.8
8,26
7.8
958,0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
1000 mg/L Nitrate Rep 1
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
24/07/2009 0 24.7 8.21 7.8 1881.0 132.0 102,0 ND
25/07/2009 1 24.2 7,9
26/07/2009 2 25.2 7.4
27/07/2009 3 24.2 8.22 7.5 2096.0 ND
28/07/2009 4
29/07/2009 5 25.8 8.76 7.4
30/07/2009 6 24.7
31/07/2009 7
01/08/2009 8
02/08/2009 9
03/08/2009 10 24.3 8.28 8.0 3570.0 ND
04/08/2009 11
05/08/2009 12
06/08/2009 13
07/08/2009 14
08/08/2009 15
09/08/2009 16 24,7
10/08/2009 17
11/08/2009 18 26.0
12/08/2009 19 25.2 8.24 8.0
13/08/2009 20 24.4
14/08/2009 21
15/08/2009 22
16/08/2009 23 25.0
17/08/2009 24 25.0 8,06 7.7 2270.0 ND
18/08/2009 25
19/08/2009 26
20/08/2009 27 25.6
21/08/2009 28 24,7 8.17 7.8
22/08/2009 29
23/08/2009 30
24/08/2009 31
25/08/2009 32 24.6 8.00 6.9 2430.0 132.0 106.0 ND
N
16
8
10
5
2
2
Max
26.0
8.76
8.0
3570.0
132.0
106.0
Min
24.2
8.00
6.9
1881.0
132.0
102.0
Mean
24,9
8.24
7.6
2449,4
132.0
104.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Winnow)
1000 mg/L Nitrate Rep 2
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
24/07/2009
0
24,8
8.17
7,8
1895.0
25/07/2009
1
24,3
7.9
26/07/2009
2
24,8
7.4
27/07/2009
3
28/07/2009
4
23.9
29/07/2009
5
30/07/2009
6
31/07/2009
7
24.6
8,16
7,2
01/08/2009
8
23.6
02/08/2009
9
03/08/2009
10
24.6
8.31
8.0
2330,0
04/08/2009
11
25.3
05/08/2009
12
06/08/2009
13
07/08/2009
14
24,2
8.06
7.9
08/08/2009
15
24.5
09/08/2009
16
10/08/2009
17
11/08/2009
18
25.9
12/08/2009
19
25.3
8.25
8.0
13/08/2009
20
25.3
14/08/2009
21
15/08/2009
22
16/08/2009
23
24.9
17/08/2009
24
25.4
8,05
7.7
2380.0
18/08/2009
25
19/08/2009
26
25,2
8.06
6.8
20/08/2009
27
21/08/2009
28
23.9
7.78
6,0
22/08/2009
29
23/08/2009
30
25.0
24/08/2009
31
24.2
25/08/2009
32
24,5
8.07
4.3
2220.0
N
' 20
9
11
4
Max
25.9
8.31
8.0
2380,0
Min
23.6
7.78
4.3
1895.0
Mean
24 7
8,10
7.2
2206.3
ND
ND
ND
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
1000 mg/L Nitrate Rep 3
Date Test Day Temperature pH
24/07/2009 0 24.8
25/07/2009 1 24,2
26/07/2009 2 25.0
27/07/2009 3 24.8
28/07/2009 4
29/07/2009 5 25.8
30/07/2009 6
31/07/2009 7 24.4
01/08/2009 8
02/08/2009 9 25.6
03/08/2009 10
04/08/2009 11 25.6
05/08/2009 12 25.1
06/08/2009 13 24.7
07/08/2009 14 24.3
08/08/2009 15 24.3
09/08/2009 16
10/08/2009 17 24.6
11/08/2009 18
12/08/2009 19 25.2
13/08/2009 20 25.4
14/08/2009 21 25.3
15/08/2009 22 25.1
16/08/2009 23
17/08/2009 24 24.8
18/08/2009 25 25,8
19/08/2009 26
20/08/2009 27 25.7
21/08/2009 28
22/08/2009 29 24.5
23/08/2009 30 25.2
24/08/2009 31 24.4
25/08/2009 32 24.7
N 24
Max 25.8
Min 24,2
Mean 25.0
DO SO Hardness Alkallinity Ammonia
8.16
7.7
7.8
7.5
8.15
7,4
2043.0
8.52
7.4
8.28
7.6
8.35
7.5
8.04
8.0
8.27
7,9
2230.0
8.18
9.4
8.30
6.6
9
11
2
8.52
9.4
2230,0
8.04
6,6
2043.0
8.25
7.7
2136,5
-------
Dally Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
Date
24/07/2009
25/07/2009
26/07/2009
27/07/2009
28/07/2009
29/07/2009
30/07/2009
31/07/2009
01/08/2009
02/08/2009
03/08/2009
04/08/2009
05/08/2009
06/08/2009
07/08/2009
08/08/2009
09/08/2009
10/08/2009
11/08/2009
12/08/2009
13/08/2009
14/08/2009
15/08/2009
16/08/2009
17/08/2009
18/08/2009
19/08/2009
20/08/2009
21/08/2009
22/08/2009
23/08/2009
24/08/2009
25/08/2009
1000 mg/L Nitrate Rep 4
Test Day Temperature pH DO
25.2 8.23
24.2
25,1
24.3
SG
Hardness Alkallinity Ammonia
0
1
2
3
4
5
9
10
1
2
3
4
5
20
21
22
23
24
25
26
27
28
29
30
31
32
24.7
24.6
25.7
25.1
24.9
24.6
24.6
25.4
24.0
25.3
25.2
24.8
25.9
25.8
24,6
25.0
8.32
8.25
8.19
8.50
7,8
7.7
7.4
7.2
7.9 2240.0
9.4
ND
7.9
8.04
6.8
N
20
6
8
1
Max
25,9
8.50
9.4
2240.0
Min
24.0
8.04
6.8
2240.0
Mean
25,0
8.26
7.8
2240.0
-------
Dally Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
2000 mg/L Nitrate Rep 1
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
24/07/2009 0 25,2 8,16 7.8 3580.0 160.0 98.0 ND
25/07/2009 1 24.2 7.8
26/07/2009 2 25.7 7.4
27/07/2009 3 24.4 8.20 7,3 3809.0 ND
28/07/2009 4
29/07/2009 5 26.0 8.82 7.4
30/07/2009 6 25.1
31/07/2009 7
01/08/2009 8
02/08/2009 9
03/08/2009 10 25.2 8.26 8.1 3430.0 ND
04/08/2009 11
05/08/2009 12 25.2 8.35 7.2
06/08/2009 13
07/08/2009 14
08/08/2009 15 24.4
09/08/2009 16
10/08/2009 17
11/08/2009 18 26.0
12/08/2009 19 25.3 8,26 7.5
13/08/2009 20 24.4
14/08/2009 21
15/08/2009 22
16/08/2009 23 25.6
17/08/2009 24 24.7 8.06 7.7 4080.0 ND
18/08/2009 25
19/08/2009 26
20/08/2009 27 25,6
21/08/2009 28 24.7 7.60 7,6
22/08/2009 29
23/08/2009 30 25.3
24/08/2009 31
25/08/2009 32 24.5 8.24 6.4 4200,0 132,0 104.0
N
18
9
11
5
2
2
Max
26.0
8.82
8.1
4200.0
160.0
104.0
Min
24.2
7.60
6.4
3430.0
132.0
98.0
Mean
25.1
8.22
7.5
3819.8
146.0
101.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales prometas (Fathead Minnow)
2000 mg/L Nitrate Rep 2
Date Test Day
Temperature
pH
DO
SC
24/07/2009
0
25.1
8.19
7.8
3610.0
25/07/2009
1
24.2
7,8
26/07/2009
2
25.5
7.3
27/07/2009
3
28/07/2009
4
24,5
29/07/2009
5
30/07/2009
6
31/07/2009
7
24.8
8,22
7.8
01/08/2009
8
24.2
02/08/2009
9
03/08/2009
10
25.1
8.26
8.1
3540.0
04/08/2009
11
25.8
05/08/2009
12
06/08/2009
13
07/08/2009
14
24,3
8.19
8.3
08/08/2009
15
24.4
09/08/2009
16
10/08/2009
17
11/08/2009
18
26,0
12/08/2009
19
25.3
8,27
7.7
13/08/2009
20
24.3
14/08/2009
21
15/08/2009
22
16/08/2009
23
25.6
17/08/2009
24
24,7
18/08/2009
25
19/08/2009
26
25.1
8,47
7.9
20/08/2009
27
21/08/2009
28
24.6
8.11
7.7
22/08/2009
29
23/08/2009
30
25.0
24/08/2009
31
23.9
25/08/2009
32
24.4
8.16
6.9
4140.0
N
20
8
10
3
Max
26.0
8.47
8.3
4140.0
Min
23,9
8.11
6.9
3540.0
Mean
24.8
8.23
7.7
3763,3
Hardness Alkallinity Ammonia
ND
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
2000 mg/L Nitrate Rep 3
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
3941.0
24/07/2009
0
24 8
8.23
7.7
25/07/2009
1
24.2
7.6
26/07/2009
2
24.8
7.4
27/07/2009
3
24,3
8.18
7.2
28/07/2009
4
29/07/2009
5
25.4
8.22
7.0
30/07/2009
6
31/07/2009
7
24.7
8.11
6.9
01/08/2009
8
02/08/2009
9
25.3
03/08/2009
10
04/08/2009
11
25.6
05/08/2009
12
24.7
8.12
6.9
06/08/2009
13
24.5
07/08/2009
. 14
24.6
8.12
7.6
08/08/2009
15
09/08/2009
16
24.6
10/08/2009
17
24.5
8.08
7.5
11/08/2009
18
12/08/2009
19
13/08/2009
20
14/08/2009
21
25.3
8.21
9.4
15/08/2009
22
24.9
16/08/2009
23
17/08/2009
24
24.8
18/08/2009
25
25.5
19/08/2009
26
20/08/2009
27
25.7
21/08/2009
28
22/08/2009
29
24.4
23/08/2009
30
24/08/2009
31
24.0
25/08/2009
32
24.5
8.12
6.8
4060.0 ND
N
21
9
11
2
Max
25.7
8.23
9.4
4060.0
Min
24.0
8.08
6.8
3941.0
Mean
24.8
8.15
7.5
4000.5
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrate
Pimephales promelas (Fathead Minnow)
2000 mg/L Nitrate Rep 4
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
24/07/2009
0
24.6
8.15
7.8
25/07/2009
1
24,1
7.8
26/07/2009
2
25.1
7.3
27/07/2009
3
28/07/2009
4
24.6
29/07/2009
5
30/07/2009
6
25.0
31/07/2009
7
01/08/2009
8
24.6
02/08/2009
9
254
03/08/2009
10
04/08/2009
11
05/08/2009
12
06/08/2009
13
25.2
07/08/2009
14
08/08/2009
15
09/08/2009
16
24.6
10/08/2009
17
24.7
8.25
7.9
11/08/2009
18
12/08/2009
19
25.4
13/08/2009
20
14/08/2009
21
25.5
8.24
9.4
15/08/2009
22
25.5
16/08/2009
23
17/08/2009
24
25.0
18/08/2009
25
25.8
19/08/2009
26
25.8
8.28
8.1
20/08/2009
27
21/08/2009
28
22/08/2009
29
24.4
23/08/2009
30
24/08/2009
31
25/08/2009
32
25.1
8.01
6.2
N
18
5
7
1
Max
25.8
8.28
9.4
3610.0
Min
24.1
8.01
6.2
3610.0
Mean
25,0
8.19
7.8
3610.0
-------
GlkC
Sh€et
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
TEST MATERIAL:
toVrrak.
TYPE OF TEST;
PROJECT NUMBER:_
TEST SPECIES:
ipM-a-D-
DILUTION WATER:
trtflVV
NUMBER OF FISH/CHAMBER:.
NUMBER OF CHAMBERS:
"IpePfr
M,
; Dif.Mor
DILUTOR NUMBER:
TEST TEMPERATURE: &5 £ I
PHOTOPERIOD;
AGE OF FISH:
DATE
TEST
TIME
(DAYS)
TECH.
INIT.
TREATMENT
us ^ w
2SO ""J \>-
TIME
REPLICATE NUMBER
1
2
3
4
1
2
3
4
1
2
3
4
•/
#
D
/
Temperature
^,1
PM«1
55.1
asw
PH
ft-oi
&.n
I
53'
t?'W
DO
7,
-7-9
B-0
>
&3k>
Hardness
t3 ip
i?>(*
I7fr
Alkalinity
Q(p
Ifolf
lO^
Ammonia as N
1
Temperature
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£
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IT
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PH
B.-93
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DO
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Sp. Cond.
Is&fc.lff
M4v».fe
M45-a
v«%1
\P12
Hardness
/
/
y
/
/
/
/
/
y
Alkalinity
/
/
/
/
/
/
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y
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X
X
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7/3Syflj
4
Temperature
TO J
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Temperature
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ni
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1
Temperature
aft.D
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aH-t
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6 •9-N'
to-pl
DO
I.Cd
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U-
l.to
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Observaiion Key:
Dated:
oJjWfr-'
Reviewed by:_
were -k^«^
0oA
-Pn»vi $i f£U\dc*>~x /"«^pS
-------
GleC
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
Ga-eat late* Srarfronmeniai Center
TEST MATERIAL:_
PROJECT NUMBER:.
er
TYPE OF TEST:
NUMBER OF FISH/£HAMBER:
DILUTION WATER:
CHLUTOR NUMBER:_
XL
PHOTOPERIOD:_
\if-&
DATE
TEST
TIME
(DAYS*
TECH,
(Nrr.
TREATMENT
T (\y\
rm
/ L
2fSf) rwa 7
TIME
REPLICATE NUMBER
1
2
3
4
1
2
3
4
1
2
3
4
bit fa
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FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
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-------
VJTUJ^ FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
TEST MATERIAL:
PROJECT NUMBER:^
TEST SPECIES;
TYPE OF TEST: J DILUTION WAT6R:_ QccJhlin
NUMBER OF FISH/CHAMBER: DILUTOR NUMBER; P PHOTOP6ftlOP: (jiff ^
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DATE
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TIME
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TIME
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Dated: j|* Revised b^:
-------
PROJECT #: 6112-12 Date 24/07/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 0
CALIBRATION
Concentrations
ppm)
Natural log
Millivolts
0.5
-.0.6931472
-36.4
1
0
-46.7
10
2.30258509
-105.2
25
3.21887582
-129.9
50
3.91202301
-147.5
Regression Output:
Constant
-50.101779
Std Err of Y Est
2.994181
R Squared
0.997263
No, of Observations
5
Degrees of Freedom
3
X Coeffieient(s) -24.620459
Std Err of Coef. 0.744629
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
FHM 32-day
Con-MH
0
water
22.4
-2.944778
0.05261376
0.00984393
FHM 32-day
125 mg/L
0
water
22.9
-2.965086
0.05155604
0.00878621
250 mg/L
0
water
23.1
-2.973209
0.05113893
0.0083691
500 mg/L
0
water
22.4
-2.944778
0,05261376
0,00984393
1000 mg/1
0
water
22,5
-2.948839
0.05240049
0.00963067
2000 mg/1
0
water
22
-2.928531
0,05347554
0,01070571
n/a
Blank
0
n/a
27.5
-3.151922
0,04276983
0
-------
PROJECT#: 6112-12 Date 24/07/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 3
CALIBRATION
Concentrations <
ppm)
Natural log
Millivolts
0.5
-0.6931472
-47.4
1
0
-57.9
10
2.30258509
-114.2
25
3.21887582
-137.8
50
3.91202301
-156.1
Regression Output:
Constant
-60.853915
Std Err of Y Est
2.845401
R Squared
0.997383
No. of Observations
5
Degrees of Freedom
3
X Coefficients) -23.927044
Std Err of Coef. 0.70762862
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
FHM 32-day
Con-MH
3
water
-15.2
-1.908047
0.14836993
-0.0076309
FHM 32-day
125 mg/L
3
water
-13,6
-1.974917
0.1387729
-0.0172279
250 mg/L
3
water
-1.6
-2,476441
0.08404179
-0.071959
500 mg/L
3
water
-3.9
-2.380316
0.09252138
-0.0634794
1000 mg/1
3
water
0.8
-2.576746
0.07602098
-0.0799798
2000 mg/1
3
water
1.2
-2.593463
0.07476066
-0,0812401
n/a
Blank
3
n/a
-16.4
-1.857894
0.1560008
0
-------
PROJECT#: 6112-12 Date 08/03/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 10
CALIBRATION
Concentrations i
PPm)
Natural log
Millivolts
0.5
-0.6931472
-47.6
1
0
-61.0
10
2.30258509
-117.5
25
3.21887582
-139.4
50
3.91202301
-157.1
Regression Output:
Constant
-62.646355
Std Err of Y Est
1.403050
R Squared
0.999364
No. of Observations
5
Degrees of Freedom
3
X Coefficient(s) -23.954251
Std Err of Coef, 0,34892733
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Lor
Levels
Blank
FHM 32-day
Con-MH
10
water
-0.3
-2.602726
0.07407138
-0.2088258
FHM 32-day
125 mg/L
10
water
11
-3.074459
0.04621464
-0.2366826
250 mg/L
10
water
11.6
-3.099506
0.04507145
-0.2378258
500 mg/L
10
water
15
-3.241444
0.0391074
-0.2437898
1000 mg/1
10
water
18.2
-3.375032
0.03421704
-0.2486802
2000 mg/1
10
water
18.9
-3.404254
0.0332316
-0.2496656
n/a
Blank
10
n/a
-32.4
-1.262672
0.2828972
0
-------
PROJECT#: 6112-12 Date 10/08/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 17
CALIBRATION
Concentrations
ppm)
Natural log
Millivolts
0.5
-0.6931472
-46.5
1
0
-60.7
10
2.30258509
-117.7
25
3.21887582
-140.3
50
3.91202301
-158.2
Regression Output:
Constant
-62.046882
Std Err of Y Est
1.228677
R Squared
0.999529
No. of Observations
5
Degrees of Freedom
3
X Coefficient(s) -24.388716
Std Err of Coef, 0.30556212
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
FHM 32-day
Con-MH
17
water
-4,1
-2.375971
0.09292421
-0.1456889
FHM 32-day
Con-MH
17
water
12.3
-3.048413
0.04743413
-0.1911789
FHM 32-day
125 mg/L
17
water
2
-2.626087
0.07236108
-0.166252
FHM 32-day
125 mg/L
17
water
15.9
-3.196022
0.04092466
-0.1976884
FHM 32-day
250 mg/L
17
water
11.4
-3.011511
0.04921726
-0.1893958
FHM 32-day
250 mg/L
17
water
19.6
-3.347732
0.03516402
-0,2034491
FHM 32-day
500 mg/L
17
water
12
-3.036112
0.04802121
-0.1905919
FHM 32-day
500 mg/L
17
water
19.8
-3.355932
0.03487683
-0.2037362
FHM 32-day
1000 mg/1
17
water
17.2
-3.249326
0.03880036
-0.1998127
FHM 32-day
1000 mg/1
17
water
21.1
-3.409236
0.03306646
-0.2055466
FHM 32-day
2000 mg/1
17
water
36.9
-4.057076
0.01729952
-0.2213136
FHM 32-day
2000 mg/1
17
water
22.1
-3.450238
0,03173807
-0.206875
n/a
Blank
17
n/a
-27.1
-1.432912
0.23861308
0
-------
PROJECT#: 6112-12 Date 17/08/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 24
CALIBRATION
Concentrations
ppm)
Natural log
Millivolts
0.5
-0.6931472
-51.4
1
0
-61.6
10
2.30258509
-117.0
25
3.21887582
-139.6
50
3.91202301
-157.5
Regression Output:
Constant
-64.567273
Std Err of Y Est
2,720863
R Squared
0.997491
No. of Observations
5
Degrees of Freedom
3
X Coefficients) -23.370225
Std Err ofCoef. 0.67665698
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
FHM 32-day
Con-MH
24
water
-5.9
-2.510343
0.0812404
-0.0950094
FHM 32-day
Con-MH
24
water
17
-3.490222
0.0304941
-0.1457557
FHM 32-day
125 mg/L
24
water
13.8
-3.353296
0.03496892
-0.1412809
FHM 32-day
125 mg/L
24
water
20
-3.618590
0.02682046
-0.1494294
FHM 32-day
250 mg/L
24
water
19.3
-3.588638
0.02763595
-0.1486139
FHM 32-dav
250 mg/L
24
water
23.5
-3.768354
0.02309005
-0.1531598
FHM 32-day
500 mg/L
24
water
19.4
-3.592917
0.02751795
-0.1487319
FHM 32-day
500 mg/L
24
water
22.4
-3.721285
0.02420284
-0.152047
FHM 32-day
1000 mg/1
24
water
35.3
-4.273270
0.01393614
-0.1623137
FHM 32-day
1000 mg/1
24
water
28.9
-3.999417
0.01832632
-0.1579235
FHM 32-day
2000 mg/1
24
water
19.4
-3.592917
0.02751795
-0.1487319
FHM 32-day
2000 mg/1
24
water
28.3
-3.973743
0.01880292
-0.1574469
n/a
Blank
24
n/a
-24
-1.735853
0.17624982
0
-------
PROJECT#: 6112-12 Date 25/08/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 32
CALIBRATION
Concentrations >
ppm)
Natural log
Millivolts
0.5
-0.6931472
-26.6
1
0
-38.4
10
2.30258509
-95.7
25
3.21887582
-118.9
50
3.91202301
-136.0
Regression Output:
Constant
-40.935819
Std Err of Y Est
2.106157
R Squared
0.998589
No, of Observations
5
Degrees of Freedom
3
X Coefficients)
Std Err of Coef.
-24.131897
0.52378457
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
I FHM 32-day
Con-MH
32
water
-7.1
-1.402120
0.24607469
0.02050857
I FHM 32-day
Con-MH
32
water
-5.1
-1.484998
0,22650279
0.00093666
[ FHM 32-day
125 mg/L
32
water
-4.2
-1.522293
0,21821094
-0.0073552
I FHM 32-day
125 mg/L
32
water
-2.1
-1.609315
0.20002462
-0.0255415
I FHM 32-day
250 mg/L
32
water
-2.3
-1.601027
0.20168927
-0.0238769
I FHM 32-dav
250 mg/L
32
water
-0.5
-1.675617
0.18719263
-0,0383735
1 FHM 32-day
500 mg/L
32
water
-0.1
-1,692193
0.18411538
-0,0414508
FHM 32-day
500 mg/L
32
water
-1
-1.654898
0.19111162
-0.0344545
T FHM 32-day
1000 mg/1
32
water
1.9
-1.775071
0.1694715
-0.0560946
F FHM 32-day
1000 mg/1
32
water
2
-1.779214
0,16877068
-0.0567955
I FHM 32-day
2000 mg/1
32
water
-1.696337
0.183354
-0.0422121
I FHM 32-day
2000 mg/1
32
water
-1.696337
0.183354
-0.0422121
| n/a
Blank
32
n/a
-5
-1.489142
0,22556613
0
-------
Nitrate HECD B-12 32-Day FHM Toxcicity Test
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-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD B-12 with Pimephales promela (fathead minnow)
Date/Time Temferatu
07/24/09 18:35:00 2OTT
07/24/09 19:35:00 25,33
07/24/09 20:35:00 25.33
07/24/09 21:35:00 25.33
07/24/09 22:35:00 25.33
07/24/09 23:35:00 25.33
07/25/09 00:35:00 25.16
07/25/09 01:35:00 25.16
07/25/09 02:35:00 25.16
07/25/09 03:35:00 25.16
07/25/09 04:35:00 25,16
07/25/09 05:35:00 25,16
07/25/09 06:35:00 25.16
07/25/09 07:35:00 25.16
07/25/09 08:35:00 25.16
07/25/09 09:35:00 25.16
07/25/09 10:35:00 25.16
07/25/09 11:35:00 25.16
07/25/09 12:35:00 25.16
07/25/09 13:35:00 25,16
07/25/09 14:35:00 25,16
07/25/09 15:35:00 25.16
07/25/09 16:35:00 25.16
07/25/09 17:35:00 25,16
07/25/09 18:35:00 25.16
07/25/09 19:35:00 25.16
07/25/09 20:35:00 25.33
07/25/09 21:35:00 25.33
07/25/09 22:35:00 25.51
07/25/09 23:35:00 25.51
07/26/09 00:35:00 25,67
07/26/09 01:35:00 25.84
07/26/09 02:35:00 25,84
07/26/09 03:35:00 24,02
07/26/09 04:35:00 24.19
07/26/09 05:35:00 24,54
07/26/09 06:35:00 24.71
07/26/09 07:35:00 24.37
07/26/09 08:35:00 24.02
07/26/09 09:35:00 25.33
07/26/09 10:35:00 25.16
07/26/09 11:35:00 25.16
07/26/09 12:35:00 25.16
07/26/09 13:35:00 25.16
07/26/09 14:35:00 25.16
07/26/09 15:35:00 25.16
07/26/09 16:35:00 25.16
07/26/09 17:35:00 25.16
07/26/09 18:35:00 25.16
07/26/09 19:35:00 25,16
07/26/09 20:35:00 25,16
07/26/09 21:35:00 25,16
07/26/09 22:35:00 25.16
07/26/09 23:35:00 25.16
07/27/09 00:35:00 25.16
07/27/09 01:35:00 25,16
07/27/09 02:35:00 25.16
07/27/09 03:35:00 25.16
07/27/09 04:35:00 25.16
07/27/09 05:35:00 25.16
07/27/09 06:35:00 25.16
07/27/09 07:35:00 25,16
07/27/09 08:35:00 25,16
07/27/09 09:35:00 25.16
07/27/09 10:35:00 25.16
1 of 12
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD B-12 with Pimephales promela (fathead minnow)
07/27/09 11:35:00 25.16
07/27/09 12:35:00 25,16
07/27/09 13:35:00 25,16
07/27/09 14:35:00 25.16
07/27/09 15:35:00 25.16
07/27/09 16:35:00 25.16
07/27/09 17:35:00 25.16
07/27/09 18:35:00 25,16
07/27/09 19:35:00 25,16
07/27/09 20:35:00 25.16
07/27/09 21:35:00 25.16
07/27/09 22:35:00 25.16
07/27/09 23:35:00 25.16
07/28/09 00:35:00 25.16
07/28/09 01:35:00 25.16
07/28/09 02:35:00 25.16
07/28/09 03:35:00 25.16
07/28/09 04:35:00 25.16
07/28/09 05:35:00 25.16
07/28/09 06:35:00 25.16
07/28/09 07:35:00 25.16
07/28/09 08:35:00 25.16
07/28/09 09:35:00 25.16
07/28/09 10:35:00 25.33
07/28/09 11:35:00 25.33
07/28/09 12:35:00 25.33
07/28/09 13:35:00 25.33
07/28/09 14:35:00 25,33
07/28/09 15:35:00 25,16
07/28/09 16:35:00 25.16
07/28/09 17:35:00 25.16
07/28/09 18:35:00 25.16
07/28/09 19:35:00 25.33
07/28/09 20:35:00 25.33
07/28/09 21:35:00 25.33
07/28/09 22:35:00 25,33
07/28/09 23:35:00 25.51
07/29/09 00:35:00 25.51
07/29/09 01:35:00 25.67
07/29/09 02:35:00 25.67
07/29/09 03:35:00 25.67
07/29/09 04:35:00 25.67
07/29/09 05:35:00 25.67
07/29/09 06:35:00 25.67
07/29/09 07:35:00 25.67
07/29/09 08:35:00 25.67
07/29/09 09:35:00 25.67
07/29/09 10:35:00 25.67
07/29/09 11:35:00 25.84
07/29/09 12:35:00 25,51
07/29/09 13:35:00 25.51
07/29/09 14:35:00 25,33
07/29/09 15:35:00 25.33
07/29/09 16:35:00 25.16
07/29/09 17:35:00 25.33
07/29/09 18:35:00 25.33
07/29/09 19:35:00 25,33
07/29/09 20:35:00 25,33
07/29/09 21:35:00 25.33
07/29/09 22:35:00 25.51
07/29/09 23:35:00 25.51
07/30/09 00:35:00 25,33
07/30/09 01:35:00 25,33
07/30/09 02:35:00 25.51
07/30/09 03:35:00 25.33
07/30/09 04:35:00 25.33
2 Of 12
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD B-12 with Pimephales promela (fathead minnow)
07/30/09 05:35:00 25.51
07/30/09 06:35:00 25,33
07/30/09 07:35:00 25,33
07/30/09 08:35:00 25.67
07/30/09 09:35:00 25.33
07/30/09 10:35:00 25.16
07/30/09 11:35:00 25.16
07/30/09 12:35:00 25,16
07/30/09 13:35:00 25,16
07/30/0914:35:00 25.16
07/30/09 15:35:00 25,16
07/30/09 16:35:00 25.16
07/30/09 17:35:00 25.16
07/30/09 18:35:00 25.33
07/30/09 19:35:00 25,16
07/30/09 20:35:00 25.16
07/30/09 21:35:00 25.33
07/30/09 22:35:00 25,16
07/30/09 23:35:00 25,33
07/31/09 00:35:00 25.33
07/31/09 01:35:00 25.16
07/31/09 02:35:00 25.16
07/31/09 03:35:00 25.16
07/31/09 04:35:00 25.33
07/31/09 05:35:00 25.16
07/31/09 06:35:00 25.16
07/31/09 07:35:00 25.51
07/31/09 08:35:00 25,33
07/31/09 09:35:00 25,16
07/31/09 10:35:00 25.16
07/31/09 11:35:00 25.33
07/31/09 12:35:00 25.16
07/31/09 13:35:00 25.33
07/31/09 14:35:00 25.16
07/31/09 15:35:00 25.16
07/31/09 16:35:00 25,16
07/31/09 17:35:00 25.33
07/31/09 18:35:00 25.16
07/31/09 19:35:00 25.33
07/31/09 20:35:00 25.16
07/31/09 21:35:00 25.33
07/31/09 22:35:00 25.16
07/31/09 23:35:00 25.33
08/01/2009 00:35 25,16
08/01/2009 01:35 25,16
08/01/2009 02:35 25.16
08/01/2009 03:35 25,16
08/01/2009 04:35 25,16
08/01/2009 05:35 25.16
08/01/2009 06:35 25.16
08/01/2009 07:35 25.33
08/01/2009 08:35 25.16
08/01/2009 09:35 25.16
08/01/2009 10:35 25.16
08/01/2009 11:35 25.16
08/01/2009 12:35 25.16
08/01/2009 13:35 25.33
08/01/2009 14:35 25,16
08/01/2009 15:35 25.16
08/01/2009 16:35 25.16
08/01/2009 17:35 25.16
08/01/2009 18:35 25.33
08/01/2009 19:35 25.16
08/01/2009 20:35 25,33
08/01/2009 21:35 25,16
08/01/2009 22:35 25.16
3 of 12
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD B-12 with Plmephales promela (fathead minnow)
08/01/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2000
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/02/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/03/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
08/04/2009
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.33
35
25.16
35
25.16
35
25.16
35
25,16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.33
35
25.33
35
25.16
35
25.16
35
25,16
35
25.16
35
25.16
35
25.16
35
25,16
35
25.16
35
25.33
35
25.16
35
25.33
35
25.16
35
25,16
35
25.33
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.33
35
25.33
35
25.16
35
25.33
35
25.33
35
25.33
35
25.33
35
25,33
35
25.33
35
25.33
35
25.33
35
25.33
35
25.16
35
25.51
35
25.33
35
25.16
35
25.16
35
25,16
35
25.16
35
25.33
35
25,16
35
25,33
35
25.16
23
00
01
02
03
04
05
06
07
08
09
10
11
12
13
14
15
16
17
18
19
20
21
22
23
00
01
02
03
04
05
06
07
08
09
10
11
12
13
14
15
16
17
18
19
20
21
22
23
00
01
02
03
04
05
06
07
08
09
10
11
12
13
14
15
16
4 of 12
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD B-12 with Pimephales promela (fathead minnow)
08/04/2009 17
08/04/2009 18
08/04/2009 19
08/04/2009 20
08/04/2009 21
08/04/2009 22
08/04/2009 23
08/05/2009 00
08/05/2009 01
08/05/2009 02
08/05/2009 03
08/05/2009 04
08/05/2009 05
08/05/2009 06
08/05/2009 07
08/05/2009 08
08/05/2009 09
08/05/2009 10
08/05/2009 11
08/05/2009 12
08/05/2009 13
08/05/2009 14
08/05/2009 15
08/05/2009 16
08/05/2009 17
08/05/2009 18
08/05/2009 19
08/05/2009 20
08/05/2009 21
08/05/2009 22
08/05/2009 23
08/06/2009 00
08/06/2009 01
08/06/2009 02
08/06/2009 03
08/06/2009 04
08/06/2009 05
08/06/2009 06
08/06/2009 07
08/06/2009 08
08/06/2009 09
08/06/2009 10
08/06/2009 11
08/06/2009 12
08/06/2009 13
08/06/2009 14
08/06/2009 15
08/06/2009 16
08/06/2009 17
08/06/2009 18
08/06/2009 19
08/06/2009 20
08/06/2009 21
08/06/2009 22
08/06/2009 23
08/07/2009 00
08/07/2009 01
08/07/2009 02
08/07/2009 03
08/07/2009 04
08/07/2009 05
08/07/2009 06
08/07/2009 07
08/07/2009 08
08/07/2009 09
08/07/2009 10
35
25.33
35
25.16
35
25.33
35
25.33
35
25.33
35
25.51
35
25.51
35
25.51
35
25.33
35
25.33
35
25.51
35
25.51
35
25.16
35
25.33
35
25.16
35
25.16
35
25.16
35
24.99
35
25.16
35
25.16
35
25,16
35
25,16
35
25.16
35
25.16
35
25.16
35
25.33
35
25.33
35
25,33
35
25.33
35
25.33
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25,16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25,16
35
25,16
35
25.16
35
25.16
35
25.16
35
25,16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25,16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
5 Of 12
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD B-12 with Pimephales promela (fathead minnow)
08/07/2009 11:
08/07/2009 12:
08/07/2009 13:
08/07/2009 14:
08/07/2009 15:
08/07/2009 16;
08/07/2009 17:
08/07/2009 18:
08/07/2009 19:
08/07/2009 20:
08/07/2009 21:
08/07/2009 22:
08/07/2009 23:
08/08/2009 00:
08/08/2009 01:
08/08/2009 02:
08/08/2009 03:
08/08/2009 04:
08/08/2009 05:
08/08/2009 06:
08/08/2009 07:
08/08/2009 08:
08/08/2009 09:
08/08/2009 10:
08/08/2009 11:
08/08/2009 12:
08/08/2009 13:
08/08/2009 14:
08/08/2009 15:
08/08/2009 16:
08/08/2009 17:
08/08/2009 18:
08/08/2009 19:
08/08/2009 20:
08/08/2009 21:
08/08/2009 22:
08/08/2009 23:
08/09/2009 00:
08/09/2009 01:
08/09/2009 02:
08/09/2009 03:
08/09/2009 04:
08/09/2009 05:
08/09/2009 06:
08/09/2009 07:
08/09/2009 08:
08/09/2009 09:
08/09/2009 10:
08/09/2009 11:
08/09/2009 12:
08/09/2009 13:
08/09/2009 14:
08/09/2009 15:
08/09/2009 16:
08/09/2009 17:
08/09/2009 18:
08/09/2009 19:
08/09/2009 20:
08/09/2009 21:
08/09/2009 22:
08/09/2009 23:
08/10/2009 00:
08/10/2009 01:
08/10/2009 02:
08/10/2009 03:
08/10/2009 04:
35
25.16
35
25.16
35
25,16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25,16
35
25.16
35
25.16
35
25,16
35
25,16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25,16
35
25,16
35
25.16
35
25,16
35
25.16
35
25.16
35
25.16
35
25,16
35
25,16
35
25.16
35
25.16
35
25.33
35
25.33
35
25.33
35
25.33
35
25.33
35
25,33
35
25,33
35
25,16
35
25.33
35
25.33
35
25,33
35
25.33
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25.16
35
25,16
35
25,16
35
25.16
35
25.16
35
25.16
35
25,16
35
25.16
35
25.16
35
25.16
6 Of 12
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD B-12 with Pimephales promeia (fathead minnow)
08/10/2009
05:35
25.16
08/10/2009
06:35
25.16
08/10/2009
07:35
25.51
08/10/2009
08:35
25.16
08/10/2009
09:35
25.16
08/10/2009
10:35
25.16
08/10/2009
11:35
25,16
08/10/2009
12:35
25.33
08/10/2009
13:35
25.16
08/10/2009
14:35
25.16
08/10/2009
15:35
25.33
08/10/2009
16:35
25.33
08/10/2009
17:35
25.33
08/10/2009
18:35
25.16
08/10/2009
19:35
25.33
08/10/2009
20:35
25.51
08/10/2009
21:35
25.67
08/10/2009
22:35
25.51
08/10/2009
23:35
25.51
08/11/2009
00:35
25.51
08/11/2009
01:35
25.51
08/11/2009
02:35
25.51
08/11/2009
03:35
25.51
08/11/2009
04:35
25.67
08/11/2009
05:35
25.67
08/11/2009
06:35
25.67
08/11/2009
07:35
25.67
08/11/2009
08:35
25.51
08/11/2009
09:35
25.16
08/11/2009
10:35
25.16
08/11/2009
11:35
25.16
08/11/2009
12:35
25.16
08/11/2009
13:35
25,16
08/11/2009
14:35
25.16
08/11/2009
15:35
25.16
08/11/2009
16:35
25.16
08/11/2009
17:35
25.16
08/11/2009
18:35
25,51
08/11/2009
19:35
25.16
08/11/2009
20:35
25.33
08/11/2009
21:35
25.67
08/11/2009
22:35
25.84
08/11/2009
23:35
24.19
08/12/2009
00:35
24.19
08/12/2009
01:35
24.37
08/12/2009
02:35
24.37
08/12/2009
03:35
24.37
08/12/2000
04:35
24.37
08/12/2009
05:35
24.37
08/12/2009
06:35
24.37
08/12/2009
07:35
24.37
08/12/2009
08:35
24.19
08/12/2009
09:35
25.67
08/12/2009
10:35
25.51
08/12/2009
11:35
24.99
08/12/2009
12:35
25.16
08/12/2009
13:35
24.99
08/12/2009
14:35
25.16
08/12/2009
15:35
24.99
08/12/2009
16:35
24.99
08/12/2009
17:35
25.16
08/12/2009
18:35
24,99
08/12/2009
19:35
25.33
08/12/2009
20:35
25.33
08/12/2009
21:35
25.33
08/12/2009
22:35
25,33
7 of 12
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD B-12 with Pimephales promela (fathead minnow)
08/12/2009 23:35 25 33
08/13/09 00:35:00 25.33
08/13/09 01:35:00 25.33
08/13/09 02:35:00 25.51
06/13/09 03:35:00 25.33
08/13/09 04:35:00 25.33
08/13/09 05:35:00 25.33
08/13/09 06:35:00 25.33
08/13/09 07:35:00 25.67
08/13/09 08:35:00 25.16
08/13/09 09:35:00 25.16
08/13/09 10:35:00 25.16
08/13/09 11:35:00 25.16
08/13/09 12:35:00 25.16
08/13/09 13:35:00 25.16
08/13/09 14:35:00 25.16
08/13/09 15:35:00 25.16
08/13/09 16:35:00 25.33
08/13/09 17:35:00 25.33
08/13/09 18:35:00 25.33
08/13/09 19:35:00 25.33
08/13/09 20:35:00 25.16
08/13/09 21:35:00 25.33
08/13/09 22:35:00 25.33
08/13/09 23:35:00 25.33
08/14/09 00:35:00 25.33
06/14/09 01:35:00 25.16
08/14/09 02:35:00 25.16
08/14/09 03:35:00 25.33
08/14/09 04:35:00 25.51
08/14/09 05:35:00 25.33
08/14/09 06:35:00 25.33
08/14/09 07:35:00 25.16
08/14/09 08:35:00 25.67
08/14/09 09:35:00 25.51
08/14/09 10:35:00 25.33
08/14/09 11:35:00 25.33
08/14/09 12:35:00 25.33
08/14/09 13:35:00 25.33
08/14/0914:35:00 25.16
08/14/09 15:35:00 25.16
08/14/0916:35:00 25,33
08/14/0917:35:00 25.33
08/14/09 18:35:00 25.33
08/14/09 19:35:00 25.67
08/14/09 20:35:00 25.51
08/14/09 21:35:00 25.33
08/14/09 22:35:00 25,51
08/14/09 23:35:00 25.33
08/15/09 00:35:00 25.16
08/15/09 01:35:00 25.33
08/15/09 02:35:00 25,16
08/15/09 03:35:00 25,33
08/15/09 04:35:00 25,16
08/15/09 05:35:00 25,33
08/15/09 06:35:00 25.33
08/15/09 07:35:00 25.16
08/15/09 08:35:00 24.99
08/15/09 09:35:00 24.99
08/15/09 10:35:00 25,16
08/15/09 11:35:00 24.99
08/15/09 12:35:00 24.99
08/15/09 13:35:00 25.16
08/15/09 14:35:00 25.16
08/15/09 15:35:00 24.99
08/15/0916:35:00 24.99
8 of 12
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD B-12 with Pimephales promela (fathead minnow)
08/15/09
17
35:00
25.16
08/15/09
18
35:00
25.33
08/15/09
19
35:00
25.16
08/15/09
20
35:00
25,16
08/15/09
21
35:00
25.16
08/15/09
22
35:00
25.16
08/15/09
23
35:00
25.16
08/16/09
00
35:00
25.33
08/16/09
01
35:00
25.16
08/16/09
02
35:00
25.16
08/16/09
03
35:00
25.33
08/16/09
04
35:00
25.16
08/16/09
05
35:00
25.33
08/16/09
06
35:00
25.33
08/16/09
07
35:00
25.16
08/16/09
08
35:00
25.16
08/16/09
09
35:00
25.16
08/16/09
10
35:00
25.16
08/16/09
11
35:00
25,16
08/16/09
12
35:00
25.16
08/16/09
13
35:00
24.99
08/16/09
14
35:00
24.99
08/16/09
15
35:00
24,99
08/16/09
16
35:00
25.16
08/16/09
17
35:00
24.99
08/16/09
18
35:00
25,16
08/16/09
19
35:00
24.99
08/16/09
20
35:00
25.16
08/16/09
21
35:00
25.16
08/16/09
22
35:00
25.16
08/16/09
23
35:00
25.16
08/17/09
00
35:00
24.99
08/17/09
01
35:00
25.16
08/17/09
02
35:00
25.16
08/17/09
03
35:00
25.16
08/17/09
04
35:00
25.16
08/17/09
05
35:00
25.16
08/17/09
06
35:00
25.16
08/17/09
07
35:00
25.16
08/17/09
08
35:00
25.16
08/17/09
09
35:00
24.99
08/17/09
10
35:00
25.16
08/17/09
11
35:00
25.16
08/17/09
12
35:00
24.99
08/17/09
13
35:00
24.83
08/17/09
14
35:00
24.66
08/17/09
15
35:00
24.49
08/17/09
16
35:00
24.16
08/17/09
17
35:00
24.99
08/17/09
18
35:00
24.83
08/17/09
19
35:00
24.83
08/17/09
20
35:00
24.99
08/17/09
21
35:00
24.99
08/17/09
22
35:00
24.99
08/17/09
23
35:00
24.99
08/18/09
00
35:00
24.99
08/18/09
01
35:00
24.83
08/18/09
02
35:00
24,99
08/18/09
03
35:00
24.99
08/18/09
04
35:00
24.83
08/18/09
05
35:00
24,83
08/18/09
06
35:00
24.99
08/18/09
07
35:00
24,99
08/18/09
08
35:00
24.83
08/18/09
09
35:00
24.99
08/18/09
10
35:00
24.99
9 of 12
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD B-12 with Pimephales promela (fathead minnow)
08/18/0911:35:00 25.33
08/18/09 12:35:00 24.37
08/18/09 13:35:00 24.02
08/18/09 14:35:00 24.19
08/18/09 15:35:00 24.19
08/18/09 16:35:00 24.19
08/18/09 17:35:00 24.19
08/18/09 18:35:00 24.37
08/18/09 19:35:00 24.19
08/18/09 20:35:00 24.37
08/18/09 21:35:00 24.54
08/18/09 22:35:00 24.19
08/18/09 23:35:00 24.54
08/19/09 00:35:00 24.37
08/19/09 01:35:00 24.02
08/19/09 02:35:00 24.37
08/19/09 03:35:00 24,19
08/19/09 04:35:00 24.37
08/19/09 05:35:00 24.19
08/19/09 06:35:00 24.37
08/19/09 07:35:00 25,84
08/19/09 08:35:00 24,19
08/19/09 09:35:00 25,84
08/19/09 10:35:00 24.19
08/19/09 11:35:00 24.37
08/19/09 12:35:00 24.37
08/19/09 13:35:00 24.19
08/19/09 14:35:00 24.02
08/19/09 15:35:00 24,37
08/19/09 16:35:00 24.37
08/19/09 17:35:00 24.37
08/19/09 18:35:00 24.19
08/19/09 19:35:00 24.02
08/19/09 20:35:00 24.37
08/19/09 21:35:00 24.02
08/19/09 22:35:00 24.37
08/19/09 23:35:00 24.02
08/20/09 00:35:00 24,37
08/20/09 01:35:00 24.54
08/20/09 02:35:00 24.54
08/20/09 03:35:00 24.37
08/20/09 04:35:00 24.37
08/20/09 05:35:00 24.54
08/20/09 06:35:00 24.54
08/20/09 07:35:00 24.54
08/20/09 08:35:00 24.54
08/20/09 09:35:00 24.54
08/20/09 10:35:00 24.93
08/20/09 11:35:00 24.93
08/20/09 12:35:00 24.93
08/20/09 13:35:00 24,93
08/20/09 14:35:00 24.93
08/20/09 15:35:00 24.76
08/20/09 16:35:00 24.76
08/20/09 17:35:00 24.76
08/20/09 18:35:00 24.76
08/20/09 19:35:00 24.76
08/20/09 20:35:00 24.76
08/20/09 21:35:00 25.1
08/20/09 22:35:00 24.93
08/20/09 23:35:00 24,93
08/21/09 00:35:00 24.93
08/21/09 01:35:00 25,1
08/21/09 02:35:00 25,1
08/21/09 03:35:00 24.93
08/21/09 04:35:00 24,93
10 of 12
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD B-12 with Pimephales promela (fathead minnow)
08/21/09
05:35:00
25.1
08/21/09
06:35:00
25.1
08/21/09
07:35:00
25.1
08/21/09
08:35:00
25.1
08/21/09
09:35:00
25.1
08/21/09
10:35:00
25.1
08/21/09
11:35:00
25.1
08/21/09
12:35:00
25.1
08/21/09
13:35:00
25.1
08/21/09
14:35:00
25,1
08/21/09
15:35:00
25.1
08/21/09
16:35:00
25.1
08/21/09
17:35:00
25.1
08/21/09
18:35:00
25.1
08/21/09
19:35:00
24.93
08/21/09
20:35:00
25.1
08/21/09
21:35:00
25.1
08/21/09
22:35:00
25.1
08/21/09
23:35:00
25,1
08/22/09
00:35:00
25.1
08/22/09
01:35:00
25.1
08/22/09
02:35:00
25.1
08/22/09
03:35:00
25.1
08/22/09
04:35:00
24.93
08/22/09
05:35:00
25.1
08/22/09
06:35:00
25.1
08/22/09
07:35:00
25.1
08/22/09
08:35:00
25.1
08/22/09
09:35:00
25.1
08/22/09
10:35:00
25.1
08/22/09
11:35:00
25.1
08/22/09
12:35:00
25.1
08/22/09
13:35:00
25.1
08/22/09
14:35:00
25.1
08/22/09
15:35:00
25.1
08/22/09
16:35:00
25.1
08/22/09
17:35:00
25.1
08/22/09
18:35:00
25,1
08/22/09
19:35:00
25.1
08/22/09
20:35:00
25.1
08/22/09
21:35:00
25.1
08/22/09
22:35:00
25,1
08/22/09
23:35:00
25.1
08/23/09
00:35:00
25.1
08/23/09
01:35:00
25.1
08/23/09
02:35:00
25.1
08/23/09
03:35:00
25.1
08/23/09
04:35:00
25,1
08/23/09
05:35:00
25,1
08/23/09
06:35:00
25,1
08/23/09
07:35:00
25.1
08/23/09
08:35:00
25.1
08/23/09
09:35:00
25.1
08/23/09
10:35:00
25,1
08/23/09
11:35:00
25.1
08/23/09
12:35:00
25,1
08/23/09
13:35:00
25.1
08/23/09
14:35:00
25.1
08/23/09
15:35:00
25.1
08/23/09
16:35:00
25.1
08/23/09
17:35:00
25.1
08/23/09
18:35:00
25.1
08/23/09
19:35:00
25,1
08/23/09
20:35:00
25.1
08/23/09
21:35:00
25.1
00/23/09
22:35:00
25.1
11 of 12
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrate for HECD 0-12 with Pimephales promeia (fathead minnow)
08/23/09
23
35:00
25.1
08/24/09
00
35:00
25.1
08/24/09
01
35:00
25.1
08/24/09
02
35:00
25.1
08/24/09
03
35:00
25.1
08/24/09
04
35:00
25.27
08/24/09
05
35:00
25.1
08/24/09
06
35:00
25.1
08/24/09
07
35:00
25.1
08/24/09
08
35:00
25.1
08/24/09
09
35:00
25.1
08/24/09
10
35:00
25,1
08/24/09
11
35:00
25.1
08/24/09
12
35:00
25.1
08/24/09
13
35:00
25.1
08/24/09
14
35:00
25.1
08/24/09
15
35:00
25,1
08/24/09
16
35:00
25.1
08/24/09
17
35:00
25.1
08/24/09
18
35:00
25.1
08/24/09
19
35:00
25.1
08/24/09
20
35:00
25.1
08/24/09
21
35:00
25.1
08/24/09
22
35:00
25.1
08/24/09
23
35:00
25.1
08/25/09
00
35:00
25.1
08/25/09
01
35:00
25.1
08/25/09
02
35:00
25.1
08/25/09
03
35:00
25.1
08/25/09
04
35:00
25.1
08/25/09
05
35:00
25.27
08/25/09
06
35:00
25.1
08/25/09
07
35:00
25.1
08/25/09
08
35:00
25.1
08/25/09
09
35:00
25.1
08/25/09
10
35:00
25,1
08/25/09
11
35:00
25.1
08/25/09
12
35:00
25,27
08/25/09
13
35:00
25.1
08/25/09
14
35:00
25.1
08/25/09
15
35:00
25.1
08/25/09
16
35:00
25.1
08/25/09
17
35:00
25.1
08/25/09
18
35:00
25,1
08/25/09
19
35:00
25.1
08/25/09
20
35:00
25.1
08/25/09
21
35:00
25,1
08/25/09
22
35:00
25.1
08/25/09
23
35:00
25.1
08/26/09
00
35:00
25.1
08/26/09
01
35:00
25.1
08/26/09
02
35:00
25.1
08/26/09
03
35:00
25.1
08/26/09
04
35:00
25.1
08/26/09
05
35:00
25.1
08/26/09
06
35:00
25,1
08/26/09
07
35:00
25,1
08/26/09
08
35:00
25.1
12 of 12
-------
Statistical Analyses
-------
TRIMMED SPEARMAN-KARBER METHOD. MONTANA STATE UNIV
FOR REFERENCE, CITE:
HAMILTON, M.A., R.C. RUSSO, AND R.V. THURSTON, 1977.
TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING MEDIAN
LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS.
ENVIRON, SCI. TECHNOL. 11(7): 714-719;
CORRECTION 12 (4) :417 (1978).
DATE: 7/24/09 TEST NUMBER: 6112-12
CHEMICAL: NITRATE SPECIES: FHM
RAW DATA:
CONCENTRATION(MG/L
NUMBER EXPOSED:
95.90 215.00 483.00 999.001952.00
80 80 80 80 80
DURATION (HOURS
LC50 LOWER 95% LIMIT UPPER 95% LIMIT PERCENT
TRIM
32
10 . 63
339.54
309.98
371.92
-------
Title: Nitrate 32-day FHM survival HECD-B12 August 2009
Pile: no3fhs32 Transform: ARC SINE(SQUARE ROOT(Y))
Shapiro - wilk's Test for Normality
D = 0.2803
W = 0.9580
Critical W = 0.8680 (alpha =0.01 , N = 20)
W = 0.9050 (alpha =0.05 , N = 20)
Data PASS normality test (alpha = 0.01). Continue analysis.
Title: Nitrate 32-day FHM survival HECD-B12 August 2009
File: no3fhs32 Transform: ARC SINE(SQUARE ROOT(Y))
Hartley's Test for Homogeneity of Variance
Calculated H statistic (max Var/min Var) = 8.2830
Table H statistic = 151.00 (alpha = 0.01)
50.70 (alpha = 0.05)
Used df = 3
Based on R (# groups) = 5
Data PASS homogeneity test (alpha « 0.01). Continue analysis.
Title: Nitrate 32-day FHM survival HECD-B12 August 2009
File: no3fhs32 Transform: ARC SINE(SQUARE ROOT(Y)
Bartlett1s Test for Homogeneity of Variance
Calculated B1 statistic = 3.4115 (p-value = 0.4915)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B = 13.2767 (alpha = 0.01, df = 4)
= 9.4877 (alpha = 0.05, df = 4)
-------
Title: Nitrate 32-day FHM survival HECD-B12 August 2009
File; no3fhs32 Transform: ARC SINE(SQUARE ROOT(Y))
Number of Groups: 5
C-RP IDENTIFICATION REP VALUE TRANS VALUE
1
control
1
0
9000
1.2490
1
control
2
0
9000
1,2490
1
control
3
1
0000
1.4588
1
control
4
0
7500
1. 0472
2
95.9 mg/L
1
0
9000
1.2490
2
95.9 mg/L
2
0
7500
1.0472
2
95.9 mg/L
3
0
9000
1.2490
2
95.9 mg/L
4
0
9000
1.2490
3
215 mg/L
1
0
9500
1.3453
3
215 mg/L
2
1
0000
1.4588
3
215 mg/L
3
1
0000
1.4588
3
215 mg/L
4
0
7500
1.0472
4
483 mg/L
1
0
1500
0.3977
4
483 mg/L
2
0
2000
0.4636
4
483 mg/L
3
0
2000
0.4636
4
483 mg/L
4
0
1000
0.3218
5
999 mg/L
1
0
0500
0.2255
5
999 mg/L
*¦»
0
2000
0.4636
5
999 mg/L
3
0
1000
0.3218
5
999 mg/L
4
0
0500
0.2255
Title: Nitrate 32-day FHM survival HECD-B12 August 2009
Pile: no3fhs32 Transform: ARC SINE(SQUARE ROOT(Y))
Summary Statistics on Transformed Data TABLE 1 of 2
3RP
IDENTIFICATION
N
MIN
MAX
MEAN
1
control
4
1.0472
1.4588
1.2510
2
95.9 mg/L
4
1.0472
1.2490
1.1986
3
215 mg/L
4
1.0472
1.4588
1.3275
4
4 83 mg/L
4
0.3218
0.4636
0.4117
5
999 mg/L
4
0,2255
0.4636
0.3091
Title: Nitrate 32-day FHM survival HECD-B12 August 2009
File: no3fhs32 Transform: ARC SINE(SQUARE ROOT(Y))
Summary Statistics on Transformed Data TABLE 2 of 2
GRP IDENTIFICATION VARIANCE SD SEM C.V. %
-------
1 control
2 95.9 mg/L
3 215 mg/L
4 483 mg/L
5 999 rng/L
0.0202 0.1680
0.0102 0.1009
0,0378 0.1944
0.0046 0.0675
0.0127 0.1126
0.0840 13.4320
0.0505 8.4203
0.0972 14.6422
0.0338 16.4051
0.0563 36.4191
Title: Nitrate 32-day FHM survival HECD-B12 August 2009
File: no3fhs32 Transform: ARC SINE(SQUARE ROOT(Y))
ANOVA Table
SOURCE
DF
SS
MS
F
Between
4
3.9309
0 . 9827
52.5872
Within (Error)
15
0.2803
0 . 0187
Total
19
4 .2112
(p-value = 0.0000}
Critical P = 4.8932 {alpha = 0.01, df = 4,15)
= 3.0556 (alpha = 0.05, df = 4,15)
Since F > Critical F REJECT Ho: All equal (alpha = 0.05)
Title: Nitrate 32-day FHM survival HECD-B12 August 2009
File: no3fhs32 Transform; ARC SINE(SQUARE ROOT(Y))
Dunnett's Test - TABLE 1 OF 2 Ho:Contrcl
-------
1 control 4
2 95,9 mg/L 4 0.1725 19.1 0,0250
3 215 mg/L 4 0.1725 19.1 -0.0375
4 483 mg/L 4 0.1725 19.1 0.7250
5 999 mg/L 4 0.1725 19.1 0.7875
-------
Title: Nitrate 32 day life stage FHM growth sept 2009
File: N03FHG3 2. Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
D = 5.8610
W = 0.9327
Critical W = 0.8680 (alpha = 0.01 , N = 20)
W = 0,9050 (alpha = 0,05 , N = 20),
Data PASS normality test (alpha = 0.01). Continue analysis,
Title: Nitrate 32 day life stage FHM growth sept 2009
File: N03FHG3 2, Transform: NO TRANSFORMATION
Hartley's Test for Homogeneity of Variance
Calculated H statistic (max Var/min Var) = 8.1808
Table H statistic = 151,00 (alpha = 0.01)
50,70 (alpha = 0.05)
Used df = 3
Based on R (# groups) = 5
Data PASS homogeneity test (alpha = 0.01). Continue analysis,
Title: Nitrate 32 day life stage FHM growth sept 2 009
File: N03FHG3 2. Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated B1 statistic = 2.7515 (p-value = 0.6002)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B - 13.2767 (alpha = 0.01, df = 4)
= 9.4877 (alpha = 0.05, df = 4)
-------
NO TRANSFORMATION
GRP IDENTIFICATION REP VALUE \ TRANS VALUE
1
control
1
5 .3400
1
control
2
/5.9900/014 \
5 . 990Q
1
control
3
/ 5.6500 \
5.6500
1
control
4
/ 4.7900
4.7900
2
95,5
mg/L
1
/ 6,6200
6.6200
2
95.5
mg/L
2
/ 5.6700
5.6700
2
95 . 5
mg/L
3
5.3400
5.3400
2
95 . 5
mg/L
4
5.2600 1
5,2600
3
215
mg/L
1
5.4500
5 .4500
3
215
mg/L
2
5.9800
5 . 9800
3
215
mg/L
3
5.2500 /
5 . 2500
3
215
mg/L
4
5.4600 /
5 .4600
4
483
mg/L
1
1.7800 /
1. 7800
4
483
mg/L
2
1.8400 /
1.8400
4
483
mg/L
3
\ 2.7900 /
2 .7900
4
483
mg/L
4
\ 1.2500 /
1,2500
5
999
mg/L
1
\ 0.2000 /
0.2000
5
999
mg/L
2
\ 2,02 00 /
2.0200
5
999
mg/L
3
X04 0O/
1.0400
5
999
mg/L
4
O
K
o
0.1000
Title: Nitrate 32 day life stage FHM growth sept 2009
File: N03FHG32. Transform: . - NO TRANSFORMATION
Summary Statistics on Data TABLE 1 of 2
GRP IDENTIFICATION N MIN MAX MEAN
1
control
4
4,7900
5
9900
5
4425
2
95.5 mg/L
4
5 ,2600
6
6200
5
7225
3
215 mg/L
4
5.2500
5
9800
5
5350
4
4 83 mg/L
4
1,2500
2
7900
1
9150
5
999 mg/L
4
0.1000
2
0200
0
8400
Title: Nitrate 32 day life stage FHM growth sept 2009
File: N03FHG32. Transform: NO TRANSFORMATION
Summary Statistics on Data TABLE 2 of 2
GRP IDENTIFICATION VARIANCE SD SEM C.V. %
-------
1 control
2 95.5 mg/L
3 215 mg/L
4 483 mg/L
5 999 mg/L
0.2597 0.5096
0,3895 0.6241
0.0974 0.3120
0.4106 0.6408
0.7965 0.8925
0.2548 9.3633
0.3120 10.9059
0.1560 5.6375
0.3204 33.4598
0.4462 106.2485
Title: Nitrate 32 day life stage FHM growth sept 2009
File: N03FHG32. Transform: NO TRANSFORMATION
ANOVA Table
SOURCE
DF
SS
MS
F
Between
4
86.7098
21.6775
55.4794
Within (Error)
15
5 .8609
0.3907
Total
19
92.5708
(p-value = 0.0000)
Critical F = 4.8932 {alpha = 0.01, df = 4,15)
= 3.0556 {alpha = 0.05, df = 4,15)
Since F > Critical F REJECT Ho: All equal (alpha = 0.05)
Title: Nitrate 32 day life stage FHM growth sept 2009
File: N03FHG3 2. Transform; NO TRANSFORMATION
Dunnett's Test - TABLE 1 OF 2 Ho:Control
-------
1 control 4
2 95.5 mg/L 4 1.0431 19.2 -0.2800
3 215 mg/L 4 1.0431 19.2 -0.0925
4 483 mg/L 4 1.0431 19.2 3.5275
5 999 mg/L 4 1.0431 19.2 4.6025
-------
no3 32 day fhm embryo larval survival LC 25, 20,
6
3
3
3
3
3
3
control
80
9
0
22 mg/L
80
11 , .
22 . 0
4 9 mg/1 if
80
6
49. 0
109 mg/1
80
67
109
226 mg/1
80
72
226
441 mg/1
80
80
441
-------
Title: no3 32 day fhra embryo larval survival LC 25, 20, 10
File: N03FHMLC. Transform: NO TRANSFORMATION
Probit Analysis - not Using Smoothed Proportions
DOSE
NUMBER
SUBJECTS
NUMBER
OBSERVED
OBSERVED
PROPORTION
PREDICTED
PROPORTION
22
49
109
226
441
00
00
00
00
00
Est. Mil =
sd =
80
80
80
80
80
98.0057
6.0817
11
6
67
72
80
0 ,1375
0.0750
0.8375
0,9000
1.0000
1401
2431
5621
9655
0000
Est. Sigma
sd
70.3770
Chi-Square lack of fit £^47.2636
Table Chi-square = "11.3449
Table Chi-square = 7.8147
6.6067 -TV P
Likelihood lack of fit = 50.1579
(alpha =0.01, df = 3)
(alpha = 0.05, df = 3)
Title: no3 32 day fhm embryo larval survival LC 25, 20, 10
File: N03FHMLC. Transform: NO TRANSFORMATION
Probit LC Estimates
WITHOUT CONTROL DATA
POINT EST. END POINT 95% CONFIDENCE LIMITS
LC 1
-65.7158
-96.1096
-35.3219
LC 5
-17.7542
-40.2670
4.7586
LC10
7.8139
-10.8461
26.4739
LC2 0
38 . 7749
24.0998
53.4499
LC2 5
50.5371
37.0499
64.0243
LC3 0
61.0999
48.4568
73.7430
LC4 0
80 . 1759
68.3795
91.9722
LC50
98 . 0057
86.0855
109.9258
LC60
115 . 8355
102.9300
128.7409
LC70
134.9114
120.1956
149.6272
LC7 5
145 . 4742
129.5104
161.4381
LC80
157.2365
139.7331
174.7399
LC90
188 .1975
166.1344
210 .2605
LC95
213 .7656
187.5988
239.9323
LC99
261.7271
227.4437
296.0105
-------
QUJUHjUfA1/,
MED Toxic Response Analysis Model 10/21/2010 12:31
.2?
JZ1
CO
CO
>
I
LLJ
Log(Exposure Variable}
Parameter Summary (Logistic Equation Regression Analysis)
Parameter
Guess
Final Est
StdError
95%LCL
95%UCL
LogX 50
2,017
1.9240
0,0434
1.8324
2.0155
S
1.0181
3.054
0.991
0.964
5.144
Y0
17.875
18.215
0.947
16.217
20.213
Effect Concentration Summary
% Effect
Xp Est
95%LCL
95%UCL
50.0
83.94
67.99
103.63
20.0
64.64
44.68
93.51
10.0
55.48
34.72
88.65
25.0
68.24
48.83
95.36
10/21/2010 12:31
MED Toxic Response Analysis Model, Version 1,03
-------
MED Toxic Response Analysis^Model 10/21/2010 12:31
Regression Analysis of Variance
Source
df
SS
MS
F
Alpha
Total
19
3.2630
0.17174
Regression
2
3.1186
1.55930
184.
0.0000
Error
17
0.1444
0.00849
Data Summary
Exposure
Obs Effects
Pred Effects
Residual
Weight
1.3424
18.0000
18,2001
0.2001
20,0000
1.3424
15.0000
18.2001
3.2001
20.0000
1.3424
18.0000
18.2001
0.2001
20.0000
1.3424
18,0000
18.2001
0.2001
20.0000
1.6902
19.0000
17,2247
-1.7753
20.0000
1.6902
20.0000
17.2247
-2.7753
20.0000
1.6902
20,0000
17.2247
-2,7753
20.0000
1.6902
15,0000
17.2247
2,2247
20.0000
2.0374
3,0000
3.6431
0.6431
20.0000
2.0374
4,0000
3.6431
-0.3569
20.0000
2.0374
4,0000
3.6431
-0.3569
20.0000
2.0374
2,0000
3.6431
1.6431
20.0000
2.3541
1.0000
0.0946
-0.9054
20.0000
2.3541
4.0000
0.0946
-3.9054
20.0000
2.3541
2.0000
0.0946
-1.9054
20.0000
2.3541
1.0000
0.0946
-0.9054
20.0000
2.6444
0,0000
0.0027
0.0027
20.0000
2.6444
0,0000
0,0027
0.0027
20.0000
2.6444
0,0000
0..0027
0.0027
20.0000
2.6444
0.0000
0,0027
0.0027
20.0000
10/21/2010 12:31
MED Toxic Response Analysis Model, Version 1,03
-------
MED Toxic Response Analysis Model 10/21/2010 12:31
Error Summary
Maximum Iterations Reached Without Convergence
Steepness At Maximum or Minimum Limit
10/21/2010 12:31
MED Toxic Response Analysis Model, Version 1.03
-------
QfWJlk
MED Toxic Response Analysis Model 10/21/2010 12:40
1.6 1.8 2,0 2.2
Log(Exposure Variable)
Parameter Summary (Logistic Equation Regression Analysis)
Parameter
Guess
FinalEst
StdError
95%LCL
95%UCL
LogX5Q
1.9749
1.9603
0.0453
1.8624
2,0582
S
0.9440
1.887?
0.5474
0.7053
3.0703
Y0
5.723
5.958
0.411
5.069
6.847
Effect Concentration Summary
% Effect
Xp Est
95%LCL
95%UCl
50.0
91.27
72.85
114.33
20.0
59.80
38.70
92.42
10.0
46.70
26.17
83.35
25.0
65.29
44.37
96.06
10/21/2010 12:40
MED Toxic Response Analysis Model, Version 1.03
-------
MED Toxic Response Analysis Model 10/21/2010 12:40
Regression Analysis of Variance
Source
df
SS
MS
F
Alpha
Total
15
79.76
5,317
Regression
2
72.86
36.432
68.7
0.0000
Error
13
6.89
0,530
Data Summary
Exposure
Obs Effects
Pred Effects
Residual
Weight
1.3424
6.6200
5.9026
-0.7174
1.
1.3424
5.6700
5.9026
0,2326
1.
1.3424
5.3400
5.9026
0.5626
1.
1.3424
5.2600
5.9026
0.6426
1,
1.6902
5.9800
5.2723
-0.7077
1.
1.6902
5.4600
5.2723
-0.1877
1.
1,6902
5.4500
5,2723
-0.1777
1.
1.6902
5.2500
5.2723
0.0223
1.
2.0374
2.7900
2.1353
-0.6547
1.
2.0374
1.8400
2.1353
0.2953
1.
2.0374
1.7800
2.1353
0.3553
1,
2.0374
1.2500
2.1353
0.8853
1.
2.3541
2.0200
0.2897
-1,7303
1,
2.3541
1.0400
0.2897
-0,7503
1.
2,3541
0.2000
0.2897
0,0897
1,
2.3541
0,1000
0.2897
0.1897
1,
Error Summary
No Errors
10/21/2010 12:40
MED Toxic Response Analysis Mode!, Version 1.03
-------
Appendix 9: Raw Data for 96-hr Toxicity of
Nitrite on Lampsilis siliquoidea
-------
Raw Laboratory Data
-------
96 Hour Water Column Acute Test Data Sheet/ 7 Treatments
Toxicant: f4i4 n+e - So£ii,w.w (Species: Uuv»#sHfs sUfiuoI
Rate: MO x \-S* 1 U 5o»'l, dLUo-Ko^,
Water: ppA (wtg.) #C» f ^ o«\
CllltUl ^ 5-fo^ C-~ ^XU-nK«A-t La&*
Start Date/Time: I / ?/e^ 5fw
Age: s'^KXTs^Q llnitials: Ats . »,S
lenip/Light: Ic'C 1 i» ' S (Glassware: 5oml hevJkjU' H0r.it ovevt^w U> a
Survival
|
Treatment
rep
Oh
24 h
4815
72 h
961
Chem 06
24h
48h
72h
96h
A
£dv»-W?
I
S
5
S?
jIU
*5t
£5
temp
2o. 2
. >» , i-
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ft-2-.
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S
s
<;
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?-o
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<
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3>o~1
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4
5
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<
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X
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t-H
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X
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(^. s)
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ll.ll"!"*
<3
5
4
temp
>0,2.
>0 a
0^ 3
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M-Z-
2
5
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4
pH
f'O
n-°i
3
>
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4*j.
cond
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im
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-------
Analytical Chemistry Data
-------
Dave Soucek, INHS
Nitrite in Water
Analysis Date: 0915 09
Analyst: Duane Kimme
ABE Water Quality Lab
File Name: Dave12.N02
L. sHiquoidea
y *
standard cone P&aK Height
400 160.0
2.00 81.5
1,00 41,5
0.50 21.5
0.25 10.5
SUMMARY OUTPUT
llllllllllllllllllll^^
Multiple R 0,996952576
R Square 0,998905154
Adjusted R Square 0.999873538
Standard Error 0.01714711
Observation & 5
ANOVA
(g SS
MS F
S>.criiic&nce F
Regressfon
1 02^11793
B 29S117« 31627.13"
3 3.&2041E-07
Residual
3 0.0-5068207
0.000294023
Total
4 9.3
Intercept -0.03496971 0.011757305 -2.974296525 0.0588651 -0.072386703 0.00244728 -0,072386703 0.00244728 0,031693309
X Variable 1 0.02515*249 0.000141465 177.84019^3 3.92E-07 0.024708043 0.025608456 0.024708043 0.025606456 Q.02S75&854
expected
observed
Sample ID
Date'
IrvOut
N02 as N mg/L
dilution
peak height N02 as N mg/L
% recovery
1A
o»'oa'09
In
0.0
0.0
0&'09
In
9,5
10
39.5
9.6
101
1B spike
owoa'os
In
19.5
10
83.5
20.7
106
EXQC 0.8ppm
0.8
31.0
0.74
93
CONTROL
1.0
41.0
1.00
100
2A
0912/09
Out
0.0
G..0
-------
Statistical Analyses
-------
CT-TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS
MINIMUM REQUIRED TRIM IS TOO LARGE: 100.0,SO SK IS NOT CALCULABLE.
SPEARMAN-KARBER
TRIM: .00%
LC50: .000
95% LOWER CONFIDENCE: .000
95% UPPER CONFIDENCE: .000
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
9.90 20. 0. .00 .9537D-04
19.60 20. 0. .00 .9537D-04
39.10 20. 0. .00 .9537D-04
82.00 20. 0. .00 .9537D-04
158.10 20. 0. .00 .9537D-04
312.80 20. 0. .00 .9537D-04
THE BINOMIAL TEST SHOWS THAT 312.80 AND +INFINITY CAN BE USED AS
STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9999 PERCENT.
THE LC50 FOR THIS DATA SET IS GREATER THAN 312.80
WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT
WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER
THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE
ANY STATISCALLY SOUND RESULTS.
DATE: September 8, 2009 TEST NUMBER: DURATION: 24 h
SAMPLE: N-N02 SPECIES: Lampsilis siliquoidea
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL ******* 312 800 ******* *******
MA A ******* ******* ******* *******
******* ******* ******* *******
SPEARMAN .000 .000 .000 .000
**** = LIMIT DOES NOT EXIST
-------
MINIMUM REQUIRED TRIM IS TOO LARGE: 100.0,SO SK IS NOT CALCULABLE.
SPEARMAN-KARBER
TRIM: .00%
LC50: .000
95% LOWER CONFIDENCE: .000
95% UPPER CONFIDENCE: .000
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
9.90 20. 0. .00 .9537D-04
19.60 20. 0. .00 .9537D-04
39.10 20. 0. .00 .9537D-04
82.00 20. 0. .00 .9537D-04
158.10 20. 0. .00 .9537D-04
312.80 20. 0. .00 .9537D-04
THE BINOMIAL TEST SHOWS THAT 312.80 AND +INFINITY CAN BE USED AS
STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9999 PERCENT.
THE LC50 FOR THIS DATA SET IS GREATER THAN 312.80
WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT
WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER
THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE
ANY STATISCALLY SOUND RESULTS.
DATE: September 8, 2009 TEST NUMBER: DURATION: 48 h
SAMPLE: N-N02 SPECIES: Lampsilis siliquoidea
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL ******* 312 800 ******* *******
MA A ******* ******* ******* *******
******* ******* ******* *******
SPEARMAN .000 .000 .000 .000
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 25.00%
LC50: 240.598
95% LOWER CONFIDENCE: 209.937
95% UPPER CONFIDENCE: 275.737
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
9.90 20. 0. .00 .9537D-04
19.60 20. 1. 5.00 .2003D-02
39.10 20. 0. .00 .9537D-04
82.00 20. 0. .00 .9537D-04
158.10 20. 2. 10.00 .2012D-01
312.80 20. 15. 75.00 .2069D+01
THE BINOMIAL TEST SHOWS THAT 158.10 AND 312.80 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 97.9104 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 244.046
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
1 .201 244.05 208.93 298.69
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
9 6.484 14.56 .00
A PROBABILITY OF 0 MEANS LESS THAN 0.001
SLOPE = 2.72
95% CONFIDENCE LIMITS: -4.21 AND 9.65
LC50= 271.58
95% CONFIDENCE LIMITS: 0 AND + INFINITY
LCI = 37.91
95% CONFIDENCE LIMITS: 0 AND 197.43
DATE: September 8, 2009 TEST NUMBER: DURATION: 72 h
SAMPLE: N-N02 SPECIES: Lampsilis siliquoidea
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 244.046 158.100 312.800 154.700
MAA 244.046 208.926 298.690 89.764
PROBIT 271 583 ******* ******* *******
SPEARMAN 240.598 209.937 275.737 65.801
NOTE: MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING.
ADJUSTMENTS WERE MADE PRIOR TO SPEARMAN-KARBER ESTIMATION.
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 6.67%
LC50: 176.541
95% LOWER CONFIDENCE: 144.980
95% UPPER CONFIDENCE: 214.973
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
9.90 20. 2. 10.00 .2012D-01
19.60 20. 2. 10.00 .2012D-01
39.10 20. 0. .00 .9537D-04
82.00 20. 2. 10.00 .2012D-01
158.10 20. 6. 30.00 .5766D+01
312.80 20. 20. 100.00 .9537D-04
THE BINOMIAL TEST SHOWS THAT 82.00 AND 312.80 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9798 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 184.290
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
3 .051 155.83 130.33 192.95
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
6 2.111 7.87 .00
A PROBABILITY OF 0 MEANS LESS THAN 0.001
SLOPE = 1.83
95% CONFIDENCE LIMITS: -.83 AND 4.49
LC50= 164.60
95% CONFIDENCE LIMITS: 0 AND + INFINITY
LCI = 8.81
95% CONFIDENCE LIMITS: 0 AND 48.89
DATE: September 8, 2009 TEST NUMBER: DURATION: 96 h
SAMPLE: N-N02 SPECIES: Lampsilis siliquoidea
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 184.290 82.000 312.800 230.800
MAA 155.828 130.334 192.949 62.615
PROBIT 164 596 ******* ******* *******
SPEARMAN 176.541 144.980 214.973 69.993
NOTE: MORTALITY PROPORTIONS WERE NOT MONOTONICALLY INCREASING.
ADJUSTMENTS WERE MADE PRIOR TO SPEARMAN-KARBER ESTIMATION.
**** = LIMIT DOES NOT EXIST
-------
Appendix 10: Raw Data for 48-hr Toxicity of
Nitrite on Chironomus dilutus
-------
Raw Laboratory Data
-------
48-HOUR STATIC ACUTE TOXICITY TEST
TEST MATERIAL:
INCUBATOR NUMBER:.
u.
PROJECT NUMBER:, TqIQ-(X
TEST SPECIES:, (V rkSUi^uo
TYPE OF TEST: fit /Yvi
NUMBER OF ORGAN ISMS/CHAMBER:
NUMBER OF CHAMBERS;
AGE OF ORGANISMS
MM -fir rk/m-S
DILUTION WATER
GLC AND/OR BATCH NUMBER:,
TEST TEMPERATURE:.
: Mod Wwi RCP-
22:
\0
LIGHT INTENSITY {10-20 (iefflr/S):_
PHOTOPERIOO: 16:8
date
TEST
DAY
TECH,
INITIALS
TREATMENT LEVEL
CONTROL
UB^/i
IU "%/L
3fo rvj/U
TIME
REPLICATE NUMBER
1
2
3
4
1
2
3
4
1
2 1 3
4
1
2 3
4
1
2
3
4
1
2
3
4
1
213 4
-A
¦4*
0
TEMPERATURE (*C)
20-:%
d&A
33-S
9&-S
pH
?-04
"? Ol
%'Q^
1?*os
•8 OS
%-03
DO (mgfl.)
nL
7S
"7 (d
7- U
1.G-
"7. '5"
1 -~1
SP. CONDUCTANCE
(jimhos/cm)
9cl&-
330
3*
2)1
HT>(e
¦3551-47*
>
.jf
1
NUMBER LIVE
s
5
€
6
s
5
•5
*>
¦5
5
5"
$
5
s
S
5
S
5
4
4
4
4
2.
3
3
<2-
OBSERVATIONS
TEMPERATURE (*C)
6flH
3H
Q\M
S*H
51-V
PH
11*
n,w
nm
-{.tl
I,?4?
T^l
do (mgn.)
n s
IS
~7<£
iS
7 C*
1,-1
-7.-?
SP. CONDUCTANCE
t/imtiosfcm)
x^f
2
NUMBER LIVE
5
5
5
5
5
*5
5
5
B
S
*3
s
5
5
4
4
i
S
3
H
3
2,
a
a
9
0
0
OBSERVATIONS
TEMPERATURE (°C)
pas
»'S
D^S
^•3
:;j '3
J^-3
PH
1-10
ns\
1%5
111
-?.«>
to&
ft- e at fV. *"»•:>
s_u#> 215
-------
Analytical Chemistry Data
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
\ of *5
GleC
Greai late* Environmental Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
CW-00-01
CW-00-02
CW-00-03
CW-00-04
CW-00-05
Date / Time
of Sample
704709
33
7/34709
J?3
33
7|34?09
7/34709
5-3
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
Mod, Hard Control -
nitrate/nitrite
Mod. Hard Control -
nitrate/nitrite
Stock 1.5 g/L - nitrite
Stock 1.5 g/L - nitrite
lOOmg/L - nitrite
Cheek the requested analysis (see definitions below)
Preservation
H2SO4
Cold
H2S04
Cold
h2so4
CW-00-06
7/24/09
5-3
125 mL
HDPE
60 mg/L - nitrite
H2SO4
Shipper:
1.
2.
Date/Time:
~?/StC£'<3>6
Receiver:
Date/Time:
—1
7-ZJ^oi
Temperature:
^ncTintUotfe - -V - '
5 = Biochemical Oxygei Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
1 = Ammoma-Nitrogeo TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NO3/NO2 -N = Nitrale/NitriterNitrogcn -
si! Abbreviations for other frwumU' r«|i.ntnl
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
NOi -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
Gt _/T
T pi
Great takes Environments? Cantor
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/23/09
Mailee Garton/Jamie Saxton
m, garton
Shipper:
Date/Time:
i/ml(f\ m>zo
Receiver:
uJ
Date/Time:
I j
i-?3< o<;
Temperature:
BODj- Biochemical Oxygen Demand-5 day " IDS = Total Dissolve Solids TP = Total fhnsphoms
NHt -N = Ammonia-Nitrogen " TKN = Total KjeldJhl Nitrogen TSS = Total Suspended Solids
NOj/NOj-N = Nilrate/Nitnte-Nitrogeii
Abbreviations for othfr frequent]* rramslal aiukstt
NO: -N = Nitrite Nitrogen" Total N = Total Nitrogen
NOi -N = Nitrate Nitrogen ¦ SRP = Soluble Reactive Phr.»phorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
"3
Page of
7/23/09
Great Late* Efwifonroenisi Canier
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www, elec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person- Mailee Garton/Jamie Sax ton
Email Address:
Phone Number:
Collector:
m. garton
Bottle ID
CW-00-13
CW-00-14
CW-00-15
CW-00-16
CW-00-17
CW-00-18
Date / Time
of Sample
7/^/09
n
7/X/09
P3>
7/2C/09
?3>
7/24709
7/24/09
7/^4709
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
HDPF
% 1 A. 1 !
Sample Description
(Site information /
sample type)
36 mg/L - nitrite
21.6 mg/L - nitrite
12.96 mg/L - nitrite
7.78 mg/L - nitrite
Stock 41.1 g/L
nitrate
Stock 41.1 g/L
nitrate
Shipper:
Date/Time:
** <1 ^\/3>4 fo°[ 0&>1> Q
Receiver:
Date/Time:
Temperature:
BOD3 = Biochemical Oxygen Dcmand-5 day" ~ TDS.= Total Dissolve Solids TP = Total Phosphorus
NH( -N = Ammonia-Nitrogen TKN = -Total Kjeldahl Nitrogen TSS = Total Suspended Sobds
NOj/N(>2 -N" = Nitrate/Nitnte-Nitrogeo
1 Abbreviation's for other fremimtlv ri-umstfd analv.es
NOi -N = Nitrite Nitrogen TotalN= T otal Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phospberas
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page 1 of ^
GjCv-I listeH EnvUcuirr'.f. njal Con to
739 Hastings St.
Traverse City. MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
7/25/09
Mailee Garton/Jamie Sax ton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
I Alkalinity
MTJ
Q
O
m
Chloride
Chlorophyll a
.56
. ..OH':
rr: :vc"
C-.-J© ¦
" ' ..-fc.
'M..
z
ft
as
z
-• «SI '
'-Z
TDS
&
H
¦OS -
' • H
eu
-** '
« -
Ui
E'
Nitrite
CW-Q2-01
7 mm
125 mL
HDPE
Mod. Hard Control -
nitrate
h2so4
.
X
CW-02-02
l/Z&W
125 mL
HDPE
Mod. Hard Control -
nitrite
Cold
X
CW-02-03
125 mL
HDPE
100 mg/L - nitrite
h2so4
X
CW-02-04
*rfzm 1
125 mL
HDPE
60 mg/L - nitrite
H2SO4
X
CW-02-05
"i/tefo*
125 mL
HDPE
36 mg/L-nitrite
h2so4
X
CW-02-06
-r/z$/on
125 mL
HDPE
21.6 mg/L - nitrite
h2so«
X
Shipper: Date/Time:
1. ^v/hOn by 10y>
2. 1 y-^
Receiver:
Date/Time:
7*^^"
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NH3 -N " Ammonia-Nitrogen
NO3/NQ2 -N =• Nitrate/Nitrite-N'itrogen
Definitions
TDS = Total Dissolve Solids TP = Total Phosphorus
TK.N = Total Kjeldahl Nitrogen TSS - Total Suspended -Solids
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
ot *5
GleC
Gk>
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Lakes F!>-irc>imonsof Cv-
739 Hastings St
Traverse City, MI 49686
Phone: 231 -94! -2230
Fax: 231-941-2240
vvww.Elec.CQm
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
of
7/25/09
Mailee Garton/Jamie Saxton
rn. earton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below)
. Alkalinity
tf!
Q
§
Chloride
Chlorophyll a
Hardness
Z
2
- 'SSt:'.
o
¦z
"W
o
m
a
H
' ?P
0,
E-
Vt
•-H"
+">
-z-
Nitrite
CW-02-I3
125 mL
HDPE
12.96 mg/L - nitrite
Cold
--
X
CW-02-14
mstrn
125 mL
HOPE
7.78 mg/L - nitrite
Cold
. -
X
i
¦
If:;-'-
T-
Shipper: Date/Time: Receiver: Date/Time: Temperature:
, 7/nfof IQ-jO /L./y^ 7-Ztr-o^
2.
Definitions
BODj - Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NH3 -N - Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Sol ids
NOj/NOj -N ¦ =- Nitrate/Nitrite-Nitrogen
Abbreviations fqrother freauently.requested analyses
NOj -N = Nitrite Nitrogen Total N = Total "Nitrogen
NOj -N " Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
( tT Fl
w JLjX—./^iiiiui *
iai«al Lakes Envlrornnanial Center
PREPARATION OF RECONSTITUTED WATER
WATER TYPE
\
BATCH NUMBER: \7DZ^
TOTAL VOLUME; '2*
DATE PREPARED; "~7/?&/Q7
DATA BY:
SALTS
AMOUNT ADDED TO FINAL VOLUME
NOTES
NaHCO,
CaSOK ' 2H,0
MgSO*
f, *3o
KCI
-------
PROJECT#: 6112-12 Date 23/07/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 0
CALIBRATION
Concentrations i
ppm)
Natural log
Millivolts
0.5
-0.6931472
-39.8
1
0
-49.1
10
2.30258509
-107.7
25
3.21887582
-131.3
50
3.91202301
-149.6
Regression Output;
Constant
-52.948914
Std Err of Y Est
3.286102
R Squared
0.996630
No. of Observations
5
Degrees of Freedom
3
X Coefficients) -24.341788
Std Err of Coef. 0,8172274
OUTPUT
Test
Organism
GLC
Number
Test
Day
Test
Material
Milli-
volts
Natural
Log
Predicted
Ammonia
Levels
Ammonia
Minus
Blank
c. dilutus
Con-MH
0
water
23.9
-3.157078
0.04254991
0.00689001
c. dilutus
100 mg/L Nitrite
0
water
23,7
-3.148861
0.04290095
0.00724105
n/a
Blank
0
n/a
28.2
-3.333729
0.0356599
0
-------
Nitrite Day 0 Chironomus Diluius Analytical Results 7.23.09
LabSamplelD
SampleDescription
ParamlD
Results
Unfts
RL LabQualifier
AnalysisMethod
Analysis Date
SampleOate Analyst
Calibration Date
Calibration Person
HB07240057
Mod. Hard Control-nitrate/nitrite
N02
<0.0036
img/L
0.01642S U
A4500B
24/07/2009
24/07/2009 JB
7/24/2009
JB
HB07240057A
Mod. Hard Control-nitrate/nitrite
N03
0
mg/L
0.022135 J
A4500F
24/07/2009
24/07/2009 Ben Cook
7/24/2009
Ben Cook
HB07240057B
Mod. Hard Control-nitrate/nitrite
N03
0
mg/L
0.022135 J
A4500F
24/07/2009
24/07/2009 Ben Cook
7/24/2009
Ben Cook
HB0724Q058
Stock 1,5g/L-nitrite
N02
1273
mg/L
1026.5625
A4500B
24/07/2009
24/07/2009 JB
7/24/2009
JB
HB07240Q58
Stock 1,5g/L-nitrite
N03
<60
mg/L
221.35 U
A450CF
24/07/2009
24/07/2009 Ben Cook
7/24/2009
Ben Cook
HB07240059
1Q0mg/L-nitrite
N03
<5
mg/L
22.135 U
A4500F
24/07/2009
24/07/2009 Ben Cook
7/24/2009
Ben Cook
HB07240059
100mg/L-nitrite
N02
108
mg/L
82.125
A45G0B
24/07/2009
24/07/2009 JB
7/24/2009
JB
HB07240060
60mg/L-nitrite
N03
14.S
mg/L
11.0675
A4500F
24/07/2009
24/07/2009 Ben Cook
7/24/2009
Ben Cook
HB07240Q60
60mg/L-nitrite
N02
60
mg/L
20.53125
A4500B
24/07/2009
24/07/2009 JB
7/24/2009
JB
HB07240061
36mg/L-nitrite
N02
35
mg/L
16.425
A4500B
24/07/2009
24/07/2009 JB
7/24/2009
JB
HB072400B1
36mg/L-nrtiite
N03
4.3
mg/L
4.427 J
A4500F
24/07/2009
24/07/2009 Ben COOk
7/24/2009
Ben Cook
H807240062
21.6mg/I.-nilrite
N02
22.0
mg/L
8.2125
A4500B
24/07/2009
24/07/2009 JB
7/24/2009
JB
HB07240062
21.6mg/L-nitrite
N03
3,1
mg/L
4.427 J
A4S00F
24/07/2009
24/07/2009 Ben Cook
7/24/2009
Ben Cook
HB07240063
12.96mg/L-nitrite
N02
12.7
mg/L
8.2125
A450QB
24/07/2009
24/07/2009 JB
7/24/2009
JB
HB07240063
12.96mg/L-nitrite
N03
0.43
mg/L
1.10675 J
A4500F
24/07/2009
24/07/2009 Ben Cook
7/24/2009
Ben Cook
HBG7240Q64
7 V8mgfL-nitrite
N03
1.77
mg/L
0.68405
A4500F
24/07/2009
24/07/2009 Ben Cook
7/24/2009
Ben Cook
HB07240064A
7.78mg/L-nitrite
N02
9.0
mg/L
0.016425
A4500B
24/07/2009
24/07/2009 JB
7/24/2009
JB
HB07240064B
7.78mg/L-nitrite
N02
8.4
mg/L
0.016425
A4500B
24/07/2009
24/07/2009 JB
7/24/2009
JB
Page 1
-------
Nitrite Day 2 Chironomus dilutus Analytic! Resuits 7.25.09
LabSamplelD
SampleDescriptior*
ParamlD
Results
Units
RL LabQualifier AnaiysisMethod
AnalysisDali SampleOate Analyst
Calibration Date Calibration Person
HB07250090
Mod. Hard Control-nilrite
N03
<0.0053 mg/L
0.022135 U
A4500F
27/07/2009
25/07/2009 Ben Cook
7/27/2009
Ben Cook
HB07250090
Mod. Hard Control-nitrite
N02
0.0276
mg/L
0.016425
A4500B
27/07/2009
25/07/2009 JB
7/27/2009
JB
HB072S0091
10Omg/l-nitrite
N03
4.2
mg/L
6.6405 J
A4500F
27/07/2009
25/07/2009 Ben Cook
7/27/2009
Ben Cook
HB07250091
10Omg/L-riitrite
N02
105
mg/L
82.125
A4500B
27/07/2009
25/07/2009 JB
7/27/2009
JB
HB07250092
60mg/L-nrtrite
N03
<1.1
mg/L
4.427 U
A4500F
27/07/2009
25/07/2009 Ben Cook
7/27/2009
Ben Cook
HB07250092
60mg/L-nitrite
N02
62
mg/L
20.53125
A45Q0B
27/07/2009
25/07/2009 JB
7/27/2009
JB
HB07250093
36mgfl_-nitrite
N03
2.4
mg/L
2.2135
A450OF
27/07/2009
25/07/2009 Ben Cook
7/27/2009
Ben Cook
HB0725D093
36mg/L-nitrite
N02
35
mg/L
16.425
A4500B
27/07/2009
25/07/2009 JB
7/27/2009
JB
HB07250094A
21,6mg/L-nitrite
N03
1.5
mg/L
2.2135 J
A4500F
27/07/2009
25/07/2009 Ben Cook
7/27/2009
Ben Cook
HB07250094A
21,6mg/L-nitrite
N02
21.4
mg/L
8.2125
A4500B
27/07/2009
25/07/2009 JB
7/27/2009
JB
HB07250094B
21 6mg/L-nitrile
N03
<0.5
mg/L
2,2135 U
A4500F
27/07/2009
25/07/2009 Ben Cook
7/27/2009
Ben Cook
HB07250094B
21 ,6rrg/L-nitrite
N02
23.2
mg/L
8.2125
A4500B
27/07/2009
25/07/2009 JB
7/27/2009
JB
HB07250095
12,96mg/L-nitrite
N03
17.2
mg/L
2.2135
A4500F
27/07/2009
25/07/2009 Ben Cook
7/27/2009
Ben Cook
HB07250095
12.96mg/L-nitrite
N02
13.5
mg/L
8.2125
A4500B
27/07/2009
25/07/2009 JB
7/27/2009
JB
HB072500S6
7.78m g/L-nitrite
N03
0.51
mg/L
1.10675 J
A4500F
27/07/2009
25/07/2009 Ben Cook
7/27/2009
Ben Cook
HB0725009G
7.78m g/L-nitrite
N02
8.2
mg/L
4.10625
A4500B
27/07/2009
25/07/2009 JB
7/27/2009
JB
Page 1
-------
Reference Toxicant Data
-------
GlhC^ Chironomus dilutus Survival
NaCl Reference Toxicant data
2002-2009
Great Lakes Environment-! Cenhjr
Test Date
¦ LC50 * Mean +2 std * Mean -2 std
-------
Statistical Analyses
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
NITRITE C.DILUTUS 48 HOUR (24 HR)
Cone.
Number
Exposed
Number
Reap.
Observed
Proportion
Responding
Proportion
Responding
Adjusted for
Controls
Predicted
Proportion
Responding
8
5300
20
13
1000
20
22
2000
20
35
0000
20
61
0000
20
106
5000
20
0
0
0
0
4
10
0
0000
0.0000
0
0000
0
0000
0.0000
0
0000
0
0000
0.0000
0
0011
0
0000
0.0000
0
0159
0
2000
0.2000
0
1498
0
5000
0.5000
0
5297
Chi - Square for Heterogeneity (calculated)
Chi - Square Cor Heterogeneity
(tabular value at 0.05 level)
0.814
9.488
Mu
Sigma
2.011115
0.217643
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept
Slope
-4 ,240419
4.594674
2.345090
1.235742
-8.83S797,
2.172620,
0.355958)
7.016728)
Theoretical Spontaneous Response Rate
= 0.0000
-------
NITRITE C.DILUTUS 48 HOUR (24
HR)
Estimated
LC/EC Values
and
Confidence
Limits
Exposure
95% Confidence Limits
Point
Cone.
Lower
Upper
LC/EC
1 ,00
31.975
11.391
45 .324
LC/EC
5 .00
44.990
22.911
56.021
LC/EC
10.00
53.974
32.827
67.049
LC/EC
15 , 00
61.031
41.351
74 .807
LC/EC
50 . 00
102.592
83.487
156.150
LC/EC
85 .00
172.457
124.959
439.669
LC/EC
90 . 00
195.006
136.228
566.803
lc/ec
95 .00
233.947
154.473
827.635
LC/EC
99 .00
329.169
194.721
1690.518
-------
NITRITE
C.DILUTUS 48 HOUR (24 HR)
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10 +
9 +
8 +
7 +
5+ . .o
o
4+ ....
3 +
2 +
1+
O+O o
- + +• + + + + + -
EC01 EC10 EC25 EC50 SC75 EC9 0 EC9 9
-------
SPA PROSIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
NITRITE C. DILOTUS 4 8 HOUR
Cone .
Number
Exposed
Number
Resp.
Observed
Proportion
Responding
Proportion
Responding
Adjusted for
Controls
Predicted
Proportion
Responding
8
5300
20
0
0.0000
0.0000
0.0002
13
1000
20
0
0.0000
0.0000
0.0031
22
2000
20
2
0.1000
0 .1000
0.0465
35
0000
20
4
0.2000
0.2000
0.2213
61
0000
20
10
0.5000
0.5000
0.6348
106
5000
20
20
1.0000
1.0000
0.9279
Chi - Square for Heterogeneity (calculated)
Chi - Square Cor Heterogeneity
(tabular value at 0.05 level)
4 .529
9.488
Mu
Sigma
1.710605
0.216870
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept
Slope
-2.887697
4.611058
1.305578
0.769289
-5.446631,
3 .103251,
-0.328763)
6.118864)
Theoretical Spontaneous Response Rate
= 0.0000
-------
NITRITE C. DILUTUS 48 HOUR
Estimated LC/EC Values arid Confidence Limits
Exposure
95% Confidence
Limits
Point
Cone.
Lower
Upper
LC/EC
1
00
16.073
9 . 080
21.686
LC/EC
5
00
22 - 588
14.831
28.708
LC/EC
10
00
21.081
19.166
33.350
LC/EC
15
00
30.608
22.704
37.036
LC/EC
50
00
51.358
43.152
62.096
LC/EC
85
00
86.172
69.868
122.219
LC/EC
90
00
97.397
77.334
145.257
LC/EC
95
00
116 .771
89.554
188.306
LC/EC
99
00
164.100
117.112
308 .522
-------
NITRITE
Probit
10 +
9 +
8 +
7 +
6+
5+
4 +
3 +
2 +
C. DILUTUS 48 HOUR
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
O
o
O+o
- + - -
EC01
EC10 EC25 EC50
- + + - -
EC75 EC90
- + -
EC99
-------
Appendix 11: Raw Data for 96-hr Toxicity of
Nitrite on Amphinemura delosa
-------
Raw Laboratory Data
-------
96 Hoar Water Column Acute Test Data Sheet/ 6 treatments
Toxicant.: M i ^"n | Species: £. ^ jfy
Rate: o oli> Q« lu -*l O . j-HOg/ ML- So'io Sawnl J,i,l "-h' -^ v*frr\,uifecve^'v*
i Go.
Start Date/Time: s/ml^ 3^
Age: %f j?jy
[Initials:/vp t> c
Survival
1
Treatment
rep
Oh
24 li
48 h
72 h
96 h
Chem Oh
24h
48h
72h
96h
A
Cojiftvtt I
1
M
H
H
Lf
temp
IZ S
/X • 1
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0.?
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temp
1?. - r
l-i-i
t 2_. ^
li.f
tt-2-
p
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0
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pH
•?,r
7.1
1 - *7
|Z, 2-
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cond
HO fa
f
lf»S=
4<>r
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c
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D.O.
S
f»s>
-------
Analytical Chemistry Data
-------
Dave Soucek. INHS
Nitrite in Water
Analyst: Duane Kitnme
ABE Water Duality Lab
A. delosa
EPA 353,1 EPA 354,1 C-D
Sample ID Date N03+N02 as N mg/L N02-W mg/L N03-N mg/L
AN02 5/18/2009
-------
Statistical Analyses
-------
CT-TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS
SPEARMAN-KARBER
TRIM: 25.00%
LC50: 3.553
95% LOWER CONFIDENCE: 2.168
95% UPPER CONFIDENCE: 5.823
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
.70 16. 2. 12.50 .2090D+00
1.50 16. 2. 12.50 .2090D+00
3.10 16. 8. 50.00 .5982D+02
6.10 16. 11. 68.75 .1051D+02
12.70 16. 12. 75.00 .3841D+01
THE BINOMIAL TEST SHOWS THAT 1.50 AND +INFINITY CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.7910 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 3.100
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
4 .285 4.20 2.49 9.17
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
4 .195 1.00 .55
SLOPE = 1.70
95% CONFIDENCE LIMITS: .95 AND 2.45
LC50= 4.01
95% CONFIDENCE LIMITS: 2.62 AND 6.68
LCI = .17
95% CONFIDENCE LIMITS: .02 AND .46
DATE: May 14, 2009 TEST NUMBER: DURATION: 24 h
SAMPLE: N-N02 SPECIES: Amphinemura delosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 3.100 1.500 ******* *******
MAA 4.198 2.490 9.172 6.682
PROBIT 4.010 2.617 6.682 4.066
SPEARMAN 3.553 2.168 5.823 3.656
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 12.50%
LC50: 1.704
95% LOWER CONFIDENCE: 1.339
95% UPPER CONFIDENCE: 2.169
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
.70 16. 2. 12.50 .2090D+00
1.50 16. 5. 31.25 .1051D+02
3.10 16. 16. 100.00 .1526D-02
6.10 16. 16. 100.00 .1526D-02
12.70 16. 16. 100.00 .1526D-02
THE BINOMIAL TEST SHOWS THAT .70 AND 3.10 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.7894 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 1.752
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
2 .104 1.54 1.24 1.92
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
8 .175 1.00 .19
SLOPE = 4.76
95% CONFIDENCE LIMITS: 2.77 AND 6.74
LC50= 1.51
95% CONFIDENCE LIMITS: 1.18 AND 1.91
LCI = .49
95% CONFIDENCE LIMITS: .20 AND .72
DATE: May 14, 2009 TEST NUMBER: DURATION: 48 h
SAMPLE: N-N02 SPECIES: Amphinemura delosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 1.752 .700 3.100 2.400
MAA 1.535 1.237 1.921 .684
PROBIT 1.507 1.176 1.907 .731
SPEARMAN 1.704 1.339 2.169 .830
**** = LIMIT DOES NOT EXIST
-------
CT-TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS
SPEARMAN-KARBER
TRIM: 18.75%
LC50: 1.025
95% LOWER CONFIDENCE: .866
95% UPPER CONFIDENCE: 1.212
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
.70 16. 3. 18.75 .1064D+01
1.50 16. 13. 81.25 .1064D+01
3.10 16. 16. 100.00 .1526D-02
6.10 16. 16. 100.00 .1526D-02
12.70 16. 16. 100.00 .1526D-02
THE BINOMIAL TEST SHOWS THAT .70 AND 1.50 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 97.8729 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 1.025
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
1 .293 1.02 .80 1.31
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
7 .236 1.00 .99
SLOPE = 5.56
95% CONFIDENCE LIMITS: 2.86 AND 8.27
LC50= 1.02
95% CONFIDENCE LIMITS: .79 AND 1.28
LCI = .39
95% CONFIDENCE LIMITS: .14 AND .56
DATE: May 14, 2009 TEST NUMBER: DURATION: 72 h
SAMPLE: nitrite SPECIES: Amphinemura delosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 1.025 .700 1.500 .800
MAA 1.025 .802 1.309 .507
PROBIT 1.020 .794 1.278 .484
SPEARMAN 1.025 .866 1.212 .346
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 25.00%
LC50: .982
95% LOWER CONFIDENCE: .803
95% UPPER CONFIDENCE: 1.201
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
.70 16. 4. 25.00 .3841D+01
1.50 16. 13. 81.25 .1064D+01
3.10 16. 16. 100.00 .1526D-02
6.10 16. 16. 100.00 .1526D-02
12.70 16. 16. 100.00 .1526D-02
THE BINOMIAL TEST SHOWS THAT .00 AND 1.50 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 98.9365 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS .977
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
1 .370 .98 .71 1.28
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
7 .253 1.00 .99
SLOPE = 5.01
95% CONFIDENCE LIMITS: 2.49 AND 7.53
LC50= .97
95% CONFIDENCE LIMITS: .72 AND 1.22
LCI = .33
95% CONFIDENCE LIMITS: .10 AND .51
DATE: May 14, 2009 TEST NUMBER: DURATION: 96 h
SAMPLE: N-N02 SPECIES: Amphinemura delosa
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL .977 .000 1.500 1.500
MAA .977 .707 1.277 .570
PROBIT .967 .724 1.223 .499
SPEARMAN .982 .803 1.201 .397
**** = LIMIT DOES NOT EXIST
-------
Appendix 12: Raw Data for 96-hr Toxicity of
Nitrite on Sphaerium simile
-------
Raw Laboratory Data
-------
96 Hour Water Column Acute Test Data Sheet/ ? Treatments
Toxicant: MO-i KjHnte-
ISpecies: S, S t l I C
liatc: 3o*)<> oy\
Water: 6PA (kxuK.~>) ShHhl
Start Date/Time: s|(-> jog 2~f>n
Culture: Cchetj-M "V^ /oq Spn^C*^.*, Lq^.il
A^C. t j jCmo tAi t/** [Initials: ftp
Temp/Light: %ja c. In S [Glassware: i s-p w y }?eaAtert /ia.o mi 5olo+>'«»>*»
Survival
1
Treatment
rep
Oh
24 k
48 h
72 h
96 h
Chem Oh
24h
48h
T2h
96k
A
tontM
1
5
f?
S
s
=?
temp
IXA
2.1 -1
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2
5
3
s
S"
<5
pH
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1
n.5"
T. W
1-.U
3
s
!S
ST
cond
°) *41
1 .Hi
qnt
fS'Sr
%Z
T,0J
fa. to 2-
Ij,-01
t».ol
Alk
X
X
X
1 10
Hard
r%
X
X
X
to
1
=5
5
I
I
o
temp
%T,.f
7-X ,S
I
J- X. (.
1.?. ?
2
S
s
1
1
o
pH
*€ '2-
'? "i
-t' S~
T . u
in
3
S
5i
3
3
0
oond
21VD
•2-7 VC)
2-7 TD
llio
:
3
s
3
3
o
D.O.
n n
i .<*3
? Hi,
S ."70
\ ,/
Alk
1 IrO
X
X
X
ixW
Hard
g'f
X
X
X
90
Initials
/jt>
fiO
At
*t>
-------
Analytical Chemistry Data
-------
Dave Soucek, INNS
Nitrite in Water
Analysis Date: Oa-20 09
Analyst: Duane Kimme
ABE Water Quality Lab
File Name: Dave10.NO2
S. simile
y x
Std Cone Peak Height
4.00 157.5
2.00 80.0
1.00 41.0
0.50 21.0
0.25 11.0
SUMMARY OUTPUT
Multiple P 0.999984405
R Square 0.9999688!
Adjusted P. Square 0.999958414
Standard Error 0.009832982
Observations 5
d
&"£>
—m r—
ixdtiiticance h
Regression
1
9.299709937
9.2997099 96183.1305
7.39275t-0S
Residual
3
0.000290063
9.669E-05
Total
4
9.3
Intercept -0.04105169 0.006756961 -6.075466 0.00895194 -0.062555356 -0.019548024 -0 06255536 -0.019548 0.031693309
X Variable 1 0.0256208 8.2612E-05 310.13405 7.3928E-08 0.025357892 0.0258837QS 0 0253^.7892 0.0258837 0,026753854
expected
obserwd
N02 m N mf'L
Sample ID
Dale
NOSas N rng/L
dilution
peak height
% recovery
1A
0*'17/09
0.00
0.00
19 02
10
77.00
19.32
101.6
10
08''17/09
3* H4
10
153.50
38 92
102.3
1E
M'i •*
"6
100
32.50
79.16
104.0
IF
s. r
15Z 17
100
61.50
153.46
100.8
1G
f S p M
304 35
100
116.00
*. n
96.3
iCrep
i1- c i
1 '3 "2
10
79.00
19.63
104.3
1A spike
OR'17/09
1.00
42.50
1.05
104.8
CONTROL
1.00
40.50
l.uu
99.7
DW
0.00
0.0
-------
Dave Soucek, INHS
Nitrite in Water
Analysis Date; 08 24 09
Analyst: Duane Kimme
ABE Water Quality Lab
File Name: Dave11.N02
S. simile
y *
Stdconc Pe&KHssgm
4,00 160.0
2.00 82.0
1.00 42.0
0.50 .21,0
0.25 11.0
SUMMARY OUTPUT
Regression Stefefcs
Multiple R
R Square
Adjusted R Squar#
Slarderd Error
A NOVA
df SS MS F Sisnific&tnce F
Regreeston 1 9,29866699® 9.298669 2C©58.0S449 7.2fc6«3E-07
Residual 3. 0,001391001 0.0QO443S7
Total 4 aa
CoeiSa&tis Slandard Erny ; Siat P-vsgus Lower 95% L ewer 8^ i zv* - 0% Uppec £>5.0%
Intercept -0.040877712 0.014472712 -2.81CS48B2 0.067256622 -0.&S67 3*342 n > 'I1- -0M7^4 uu r 0.03169A309
X Variable 1 0.02518&S51 0.000173.853 144.771076 7.2S89aE-«? 0,024815672 0.0246 lrh~ n u c-_i23 0.02S753.854
expected
observed
Sample iD
Ditu
N02 as N mg/L
dilution
peak height
N02 as N mq.'L
% recovery
2A
r\<- 21 uu
0.0
2,5
0.02
2B
I'S-I i"
9.5
10
38
9.16
2C
i 3-1 iw
19.0
10
77.5
19.10
11»14
2D
Ob _| no
38.0
10
155.5
38.73
1"l fc
2E
if= _| 1 ^
76.1
too
35.5
85.28
Mw !
2F
i'c i ^
152.2
100
66
162.05
Hlh "
2G
"r%_| II •
304.4
100
120.5
299.22
¦ft
2CREP
I> z\ IN
19,0
10
ao
19,73
103.7
2ASPK
11° 21 IM
1.0
45.5
1.10
110,5
CONTROL
1.0
42
1.02
101.6
DW
0.0
0
-------
Statistical Analyses
-------
CT-TOX: BINOMIAL, MOVING AVERAGE, PROBIT, AND SPEARMAN METHODS
MINIMUM REQUIRED TRIM IS TOO LARGE: 100.0,SO SK IS NOT CALCULABLE.
SPEARMAN-KARBER
TRIM: .00%
LC50: .000
95% LOWER CONFIDENCE: .000
95% UPPER CONFIDENCE: .000
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
9.40 20. 0. .00 .9537D-04
19.20 20. 0. .00 .9537D-04
38.80 20. 0. .00 .9537D-04
82.20 20. 0. .00 .9537D-04
157.70 20. 0. .00 .9537D-04
296.10 20. 0. .00 .9537D-04
THE BINOMIAL TEST SHOWS THAT 296.10 AND +INFINITY CAN BE USED AS
STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9999 PERCENT.
THE LC50 FOR THIS DATA SET IS GREATER THAN 296.10
WHEN THERE ARE LESS THAN TWO CONCENTRATIONS AT
WHICH THE PERCENT DEAD IS BETWEEN 0 AND 100, NEITHER
THE MOVING AVERAGE NOR THE PROBIT METHOD CAN GIVE
ANY STATISCALLY SOUND RESULTS.
DATE: August 17, 2009 TEST NUMBER: DURATION: 24 h
SAMPLE: N-N02 SPECIES: sphaerium simile
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL ******* 296 100 ******* *******
MA A ******* ******* ******* *******
******* ******* ******* *******
SPEARMAN .000 .000 .000 .000
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 40.00%
LC50: 240.014
95% LOWER CONFIDENCE: 171.071
95% UPPER CONFIDENCE: 336.741
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
9.40 20. 0. .00 .9537D-04
19.20 20. 0. .00 .9537D-04
38.80 20. 0. .00 .9537D-04
82.20 20. 0. .00 .9537D-04
157.70 20. 6. 30.00 .5766D+01
296.10 20. 12. 60.00 .2517D+02
THE BINOMIAL TEST SHOWS THAT 82.20 AND +INFINITY CAN BE USED AS
STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9999 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 240.673
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
1 1.102 240.67 111.95 + INFINITY
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
7 .234 1.00 .84
SLOPE = 4.13
95% CONFIDENCE LIMITS: 2.13 AND 6.13
LC50= 241.53
95% CONFIDENCE LIMITS: 194.50 AND 340.52
LCI = 66.07
95% CONFIDENCE LIMITS: 22.85 AND 98.35
DATE: August 17, 2009 TEST NUMBER: DURATION: 48 h
SAMPLE: N-N02 SPECIES: sphaerium simile
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 240.673 82.200 ******* *******
MAA 240 673 111 951 ******* *******
PROBIT 241.531 194.504 340.516 146.012
SPEARMAN 240.014 171.071 336.741 165.669
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: 40.00%
LC50: 178.684
95% LOWER CONFIDENCE: 65.063
95% UPPER CONFIDENCE: 490.724
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
9.40 20. 0. .00 .9537D-04
19.20 20. 0. .00 .9537D-04
38.80 20. 0. .00 .9537D-04
82.20 20. 0. .00 .9537D-04
157.70 20. 10. 50.00 .5881D+02
296.10 20. 12. 60.00 .2517D+02
THE BINOMIAL TEST SHOWS THAT 82.20 AND +INFINITY CAN BE USED AS
STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.9999 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 157.700
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
2 .157 199.54 160.07 272.15
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
7 .195 1.00 .23
SLOPE = 3.66
95% CONFIDENCE LIMITS: 2.05 AND 5.28
LC50= 213.80
95% CONFIDENCE LIMITS: 170.11 AND 296.40
LCI = 49.54
95% CONFIDENCE LIMITS: 17.50 AND 76.24
DATE: August 17, 2009 TEST NUMBER: DURATION: 72 h
SAMPLE: N-N02 SPECIES: sphaerium simile
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 157.700 82.200 ******* *******
MAA 199.537 160.074 272.147 112.073
PROBIT 213.804 170.107 296.404 126.297
SPEARMAN 178.684 65.063 490.724 425.661
**** = LIMIT DOES NOT EXIST
-------
SPEARMAN-KARBER
TRIM: .00%
LC50: 55.658
95% LOWER CONFIDENCE: 42.992
95% UPPER CONFIDENCE: 72.055
CONC. NUMBER NUMBER PERCENT BINOMIAL
mg/L EXPOSED DEAD DEAD PROB.(%)
9.40 20. 0. .00 .9537D-04
19.20 20. 3. 15.00 .1288D+00
38.80 20. 8. 40.00 .2517D+02
82.20 20. 10. 50.00 .5881D+02
157.70 20. 19. 95.00 .2003D-02
296.10 20. 20. 100.00 .9537D-04
THE BINOMIAL TEST SHOWS THAT 19.20 AND 157.70 CAN BE USED AS STATISTICALLY
SOUND CONSERVATIVE 95 PERCENT CONFIDENCE LIMITS SINCE THE ACTUAL
CONFIDENCE
LEVEL ASSOCIATED WITH THESE LIMITS IS 99.8692 PERCENT.
AN APPROXIMATE LC50 FOR THIS DATA SET IS 82.200
RESULTS USING MOVING AVERAGE
SPAN G LC50 95% CONFIDENCE LIMIT
5 .051 53.56 42.03 68.04
****** RESULTS CALCULATED BY PROBIT METHOD
ITERATIONS G H GOODNESS OF FIT
6 .082 1.00 .24
SLOPE = 2.79
95% CONFIDENCE LIMITS: 1.99 AND 3.59
LC50= 54.99
95% CONFIDENCE LIMITS: 42.27 AND 71.22
LCI = 8.08
95% CONFIDENCE LIMITS: 3.51 AND 13.14
DATE: August 17, 2009 TEST NUMBER: DURATION: 96 h
SAMPLE: N-N02 SPECIES: sphaerium simile
METHOD LC50 CONFIDENCE LIMITS
LOWER UPPER SPAN
BINOMIAL 82.200 19.200 157.700 138.500
MAA 53.557 42.026 68.039 26.013
PROBIT 54.987 42.269 71.216 28.947
SPEARMAN 55.658 42.992 72.055 29.063
**** = LIMIT DOES NOT EXIST
-------
Appendix 13: Raw Data for 96-hr Toxicity of
Nitrite on Hyalella azteca
-------
Raw Laboratory Data
-------
GleC
96-HOUR STATIC ACUTE TOXICITY TEST
TEST MATERIAL:
PROJECT NUMBER:
TEST SPECIES: "
• Niftita
TYPE OF TEST:
INVESTIGATORS:
NUMBER OF ORGANISMS/CHAMBI
NUMBER OF CHAMBERS;
AGE OF ORGANISMS: , ,
DILUTION WATER;
mod %an1 » n&i
-S-
*c.
10 to
GLC AND/OR BATCH NUMBER:
TEST TEMPERATURE:_ 33>*
I INCUBATOR #; PHOTOPERJOD:
DATE
TEST
DAY
TECH.
INITIALS
TREATMENT LEVEL
pwd ttoed
CONTROL
1*3 *3 V-
a.ai^ic
TIME
REPLICATE NUMBER
t
2
3 4
12 3
4
i
2
3 4
1
2
3 4
1
2
3 4
1
2
3 4
0
jC
TEMPERATURE
c?a-o
P3 'O
0
PH
^06
isem
Sf-OO
S-OH
00{mv!L)
tf-'H
SP. COND,
DOfmn/L)
t-s
S .5:
% S
TEMPERATURE (°C)
\
Q3>. -3-
Q-h-J
. ^
2
fa-
NUMBER LIVE
5
5-
5
^'O
^0
l*o
143
SP, CONDUCTANCE
3iS
R1S
39£
TEMPERATURE(X)
93.1
1
9a • ^
9^. ^
OBSERVATION KEY:
DOB - Dried out on Beaker
F - Floater FS - Film on Surface
PM • Particulate Matter
DAY 0; CONTROL
DAY 4; CONTROL
DAY 0: HIGH CONCENTRATION
DAY 4: HIGH CONCENTRATION
END (mU):
START(mLs):
HARDNESS
ALKALINITY
HARDNESS
ALKALINITY
HARDNESS
ALKALMTY
10
HARDNESS
ALKAUNITY
S.5
aio
55 <5^
ioA
a
%o (§
TO
<00
REVIEWED BY
DATE:
OS.
-------
Analytical Chemistry Data
-------
CZt
VjrLtv^,
,«tLakes Environmental CetiSar
PREPARATION OF RECONSTITUTED WATER
WATER TYPE: /Tj/j/f j-kfJ
BATCH NUMBER: t7&H
DATE PREPARED: 9^1^!
TOTAL VOLUME:
DATA BY:
SO
/>Q
SALTS
AMOUNT ADDED TO FINAL VOLUME
NOTES
NaHCO,
O £25
CaSOB # 2H20
3t>
MgSO^
1 . 36
KCI
o. oS
WATER SOURCE;
RECONSTITUTED HARD WATER - WATER QUALITY
Date: % W> Chscked By: f\
_EH_
SPECIFIC CONDUCTANCE
3^
TOTAL HARDNESS
SAMPLE VOLUME
LS
DILUTED TO
so
mL TITRANT USED
mg/L CaCO.
TOTAL ALKALINITY
SAMPLE VOLUME
DILUTED TO
mL TITRANT USED
mg/L CaCO,
telt
Hardness: " Alkalinity: t y
End mis: \ ZX 5 End mis: L*' I
Start mis:. i^rn.s Start mis:. 3=2
3. 3
-------
PROJECT#; 6112-12
Nitrate Nitrite Tox
CALIBRATION
CONCENTRATIONS
(ppm)
0.5
1
10
25
50
Date
Test Day
Natural
log
-0.6931472
0
2.30258509
3.21887582
3.91202301
Regression Output:
Constant
Std Err of Y Est
R Squared
No. of Observations
Degrees of Freedom
08/06/2009
0 tox test
Millivolts
-53.0
-62.9
-119.8
-141.2
-159.8
-66.194866
2.819348
0.997345
5
3
X Coefficients)
Std Err of Coef.
-23.537499
0.70114936
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
H. azteca
100mg/LN03
0
water
3.5
-2.9610141 !
0.0517664
-0.0960736
H, azteca
lOmg/L N02
0
water
5.4
-3.0417363 !
0.047/M 9
-0.1000881
FHM
3000 mg/L N03
0
water
14.4
-3.4241049
0.03257843
-0.1152616
Blank
0
-21.2
-1.9116248
0.14783999
0
-------
HB072209_072409
LabSamplelD
SampleDescription
ParamlD
Results
Units
RL LabQualrfier
AnalysisMethod
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Parson
HB08060191
lOmg/L-nltrite
N03
<.21
mfl/L
0.8854 U
A4500F
8/7/2009
8/8/2009 Ben Cook
8/7/2009
Ben Cock
HB08060191
lOmg/L-nitrite
N02
13.0
mg/L
1.8425
A4500B
8/6/2009
8/8/2009 Jl
8/8/2008
Jl
HB08060192
6mg/L-rvitrite
N03
<.11
mg/L
0.4427 U
A4500F
8/7/2009
©8/2009 Sen Cook
8/7/2009
Ben Cook
HB08060192
6mg/L-ni?rite
N02
7.90
mg/L
0.82125
A4500B
8/8/2009
8/8/2009 Jl
8/8/2008
Jl
HB08060193
3.8mg/L-nitrita
N03
<0.05
mg/L
0.22135 U
A4500F
8/7/2009
8/8/2009 Sen Cook
8/7/2008
Ben Cook
HB0B060193
3.Smg/L-nitrite
N02
4.74
mg/L
0.657
A4500B
8/6/2009
8/6/2009 Jl
8/6/2008
Jl
HB0B060194
2.2mg/L-nitrite
N02
2.85
mg/L
0.3285
A4500B
8/6/2009
8/6/2009 Jl
8/0/2008
Jl
HBQ8080194A
2,2mg/L-nitrite
N03
<0.032
mg/L
0.13281 U
A4500F
8/7/2009
8/6/2009 Ben Cook
8/7/2008
Ben Cook
HB08080194B
2.2mg/L-nitrite
N03
<0.032
mfl/L
0,13281 U
A45Q0F
8/7/2008
8/6/2009 Ban Cook
8/7/2009
Ben Cook
HB08060195
l.3mg/L-nrtri!8
N03
<0.212
mfl/L
0.08854 U
A4500F
8/7/2009
8/6/2009 Ben Cook
8/7/2008
Ben Cook
HB08060195
1.3mg/L-nitrite
N02
1.683
mg/L
0.16425
A4500B
8/8/2009
8/8/2009 Jl
8/6/2009
Jl
HB0806C196
Stock O.ISfl/L NaN02 100mg/l nftrr N03
<2.7
mg/L
11.0875 U
M500F
8/7/2009
8/8/2009 Ben Cook
8/7/2009
Ben Cook
HB0B060196
Stock 0.1SQ/L NaN02100mg/L
nitri* N02
123
mfl/L
32.85
A4500B
8/8/2009
8/8/2009 Jl
8/6/2009
Jl
HB08Q60197
Mod. Hart Control-nitrate/nitrite
N03
<0.0053 fflfl/L
0.022135 U
A4500F
8/7/2009
8/6/2009 Ben Cook
8/7/2009
Ben Cook
HB08060197
Mod. Mart Controt-nitnate/nitnts
N02
<0.0036 mg/L
0.016425 U
A4500B
mam
8/6/2009 Jl
8/6/2000
Jl
Page 1
-------
Nitrite Day 4 Hyalella arteca Analytical Results 8,10.09
LsbSamptelD
SampleDescfiptlon
ParamID
1
1
c
i
RL LabQualifef
AnalystsMsthod
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Person
HB08110261
lOmg/L-mtritB-Day 4
N03
<.21
mg/L
0,8854 U
A45QQF
8/13/2009
8/11/2008 Ben Cook
8/13/2009
Ben Cook
HB08110261
lOmg/L-nitrite-Day 4
N02
14.1
mg/L
1.6425
A4500B
8/12/200S
8/11/2009 Jl
8/12/2009
Jl
HB08110262
6mg/L-nitrite-Day 4
N03
<.11
mg/L
0.4427 U
A4500F
8/13/2008
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB08110262
Bmg/L-nitrite-Day 4
N02
8.11
mg/L
0.82125
A4500B
8/12/2009
8/11/2009 Jl
8/12/2009
Jl
BB08110263
3,8mg/L-nitritB-Day 4
N03
<0,05
mg/L
0.22135 U
A4500F
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Code
HB08110263
3.8mg/L-nitritB-Day 4
N02
4.71
mg/L
0.82125
A4500B
8/12/2009
8/11/2009 Jt
8/12/2009
Jl
KB08110264
2,2mg/L-nitrite-Day 4
N03
0.245
mg/L
0.110675
A4SOOF
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB0811Q264A
2.2mg/L-nitrite-Day 4
N02
2.56
mg/L
0.3285
A45008
8/12/2009
8/11/2009 Jl
8/12/2009
Jl
HB08110264B
2.2mg/L-nitritB-Day 4
N02
2.59
mg/L
0,3285
A4500B
8/12/2009
8/11/2009 Jl
8/12/2009
Jl
HB08110265
1.Smg/L-nitrite-Day 4
N03
1.888
mg/L
0,08854
A4500F
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB08110266
1.3mg/L-nitrita-Day4
NQ2
<0.0038
mg/L
0.016425 U
A45Q0B
8/12/2009
8/11/2009 Jl
8/12/2009
Jl
HB08110254A
Mod. Hart Control-nitrati/rttrite
N03
0.248
mg/L
0.08854
A45Q0F
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB081102S4A
Mod. Hard Control-nitrate/nitrite
N02
0.0659
mg/L
0.016425
A4500B
8/12/2009
8/11/2009 Jt
8/12/2009
Jl
HB08110254B
Mod. Hard Contral-nitrate/nitrlte
N03
0.196
mg/L
0.08854
A4500F
8/13/2009
8/11/2009 Ben Cook
8/13/2009
Ben Cook
HB081102548
Mod, Hard Contral-nitrate/nitrite
NQ2
0.0684
mg/L
0.016425
A4500B
8/12/2009
8/11/2009 Jl
8/12/2009
Jl
Pagel
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page
of
Ccmof
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax:231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility;
T Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8.6.09
Mailee Garton/Jamie Sax ton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
; Alkalinity
x
0
0
a
Chloride
Chlorophyll a
Hardness
Z
I
x
z
z
O
z
fry
O
Z :
TDS
b
H
vi
¦ -fa-
a*- -s
.
. .¦+*-•; ;
-
'W'
Nitrite
HAN02W
-00-01
8/6/09
125 mL
HOPE
10 mg/L — nitrite
Cold
X
HAN02W
-00-02
8/6/09
125 ml
HOPE
6 mg/L - nitrite
Cold
-Vt
•-
X
HAN02W
-00-03
8/6/09
125 mL
HOPE
3.6 mg/L-nitrite
Cold
X
HAN02W
-00-04
8/6/09
125 mL
HOPE
2.2 mg/L - nitrite
Cold
X
HAN02W
-00-05
8/6/09
125 mL
HDPE
1.3 mg/L-nitrite
Cold
X
HAN02W
-00-06
8/6/09
125 mL
HDPE
10 mg/L - nitrite
h2so4
X
Shipper:
Date/Time:
B0Ds = Biochemical Oxygen Demand-S day
NH3 -N = Ammonia-Nitrogen
NO3/NQ2 -N =Nitratc/Nitrite-Nitrogea
' Definitions r'v'"-*' >
TDS = Total Dissolve Solids
TKN = Total KjddahlNitrogen
Receiver:
Date/Time:
y-G-oS
Temperature:
TP -Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for'other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
NOs -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
2
Page of
739 Hastings St
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
www.elec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8.6.09
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalinity
BODg
::
Chloride
Chlorophyll a
Hardness
X
z
r
0
¦ m.
**> - "
.
"Z.
Vj
Q
1-
'"p*'
s-
H
-05
: -K"
. +*
•«; .
z ;
im
g
HAN02W-
00-07
8/6/09
125 mL
HDPE
6 mg/L - nitrite
H2SO.j
x-.'. ••
HAN02W-
00-08
8/6/09
125 mL
HDPE
3.6 mg/L - nitrite
h2so4
ASS -
- * '
i
ZT-if-;,:
HAN02W-
00-09
8/6/09
125 mL
HDPE
2.2 mg/L - nitrite
h2so<
-
X -
HANOjSW-
00-10 *
8/6/09
125 mL
HDPE
1.3 mg/L-nitrite
h2so4
•.
-v. jf
Xs\ :
HANOI W-
00-11 2
8/6/09
125 mL
HDPE
Stock 0.15 g/L nano2-
100 mg/Lnitrite
H2S04
'•
¦... -
ji-
•
V,M
X
HANOjW-
00-12 a
8/6/09
125 mL
HDPE
Stock 0.15 g/L nano2-
100 mg/Lnitrite
H2S04
x,:.4
Shipper:
Date/Time:
tlyjo 1
BODs = Biochemical Oxygen Demand-5 day
NHj -N - Ammonia-Nitrogen
NOj/NOj -N =Nitrate/Nitrite-Nitrogen
Receiver:
g/
Date/Time:
3s
Temperature:
TDS = Total Dissolve Solids TP — Total Phosphorus
TK.N = Total Kjeldahl Nitrogen TSS- Total Suspended Solids
for other frequently requested *nalvs«a
NO> -N = Nitrite 'Nitrogen Total N = Total Nitrogen
NO3 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphonis
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page.
of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.coii]
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mai lee Gartori/Jamie Saxton
m. garton
Bottle ID
Date t Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
i?
.,5
"Is'
s
to
Chloride
Chlorophyll a
Hardness
Z
1
l*S
s
z
z
0
Z
. -.r*i ¦
O
Z;
2.
Date/Time:
ism
Receiver:
Date/Time:
Temperature:
BOD5 = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
N0i/N02 -N = Nitrate/Nitrite-Nitrogen
' Definitions
TDS = Total Dissolve Solids
TKN - Total Kjddahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreyiationsifor other frequently requested analyses
NOj -N - Nitrite Nitrogen Total N = Total Nitrogen
NOj -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page ^ of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240'
www.glec.com
Great Lakes Environmental Center
Facility:
r ,. Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamle Saxton
m. carton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalinity
<*)
o
o
CO
Chloride
Chlorophyll a
1
«
a
£
¦
w
'Z
' '-I
.0
z
i*i
o
,-z
TDS
H
Om
H
.. ¦.
1
Nitrate
| Nitrite
HAN02W-
04-07
8 £H0O9
VO
125 mL
HOPE
6 mg/L - nitrite-Day4
Cold
X
HAN02W-
04-08
%/yCm
P
125 mL
HDPE
3.6 mg/L-nitrite-
Day4
Cold
X
HAN02W-
04-09
8/>f/09
id
125 mL
HOPE
2.2 mg/L - nitrite-
Day4
Cold
X
HAN03W-
04-10
8/Vf/09
fo
125 mL
HDPE
1.3 mg/L - nitrite-
Day4
Cold
'
X
¦kit:,-'
ft;
' .'
m"' -
- .
;
!i
¦=¦ :->4
Shipper:
4^1
2. U
Date/Time:
YlusJ c>q
Receiver:
iU 0/1
BOD5 = Biochemical Oxygen Deraand-5 day
NH3 -N = Ammonia-Nitrogen
N0j/H02 -N - Nitratc/Nitrite-Nitrogen
Date/Time:
X-lt-cfj
Temperature:
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP - Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analysts
NO2 -N = Nitrite Nitrogen Total N'Total N itrogen
"NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
TABLE 3
Analytical and Daily Chemistry Data for the 96 Hour Acute Toxicity Test
HECD B-12 Nitrite
Hyalelts azleca
Nominal (and Measured) T«M
Concentrations
Nitrite*
Nitrate*
Temperature
...PH
{s.u.»
O.O.
(mfl/L)
Conductivity
{mtnhos)
Alkalinity
Hardness
Ammonia
(«*>
m
{mg/L.}
(mo/Li
Imo/L)
Dilution water/Control
DayO
<0.0036
<0.0963
22.9
8.00
8.4
300
60
SO
ND
Day 1
22.1
7.99
8.5
Day 2
23.2
7.7 T
8.3
Day 3
22.8
7.96
8.5
Day 4
0.07
0,196
22.1
7.00
8.0
290
60
80
0.07
0,248
0.07
.
la^eaim^L
Day 0
1.68
<0,0212
22,0
7.S3
8.4
336
Day 1
22.2
7.97
8.5
Day 2
23.2
7,99
8,4
Day 3
22.8
7.38
8,5
Day 4
1.87
<0.0036
22.1
7.91
8,0
310
1J7
2 2 (2.56) mat
Daw 0
2.8S
<0.032
22.0
7.99
8.4
340
<0.032
Day 1
22.2
7.92
8.5
Day 2
23.1
8.01
8.4
Day 3
22.7
8.04
8.5
Day 4
2.55
0,245
22.1
7.90
8.0
315
2.59
2.66
j
3 6 (4 73) m&'L
DayO
4.74
<0.05
22.0
8.00
8.4
340
Day1
22.2
7.95
8.5
Dav2
23.1
8.06
8.5
Day 3
22,8
8.06
8.6
Day 4
4.71
<0.05
22.2
7J0
8.0
325
4,73
6.0 (8 01) mgrt.
DayO
7.90
<0.11
22.0
8.04
8,4
341
Day 1
22.2
7.90
8.5
D»v2
23.1
8.06
8,5
Day 3
22,8
«.04
8.6
Day 4
8.11
<0.11
22,2
7,96
8.0
325
8.01
; - i :
10 (13.55) mg/t
DayO
13,00
<0,21
22.0
B.OS
8,4
342
60
80
ND
Day 1
22,2
7,90
8.5
Day 2
23.1
6,07
8,6
Day 3
22.8
8.04
8,5
Day 4
14.10
<0,21
22.2
7.97
8,0
322
58
80
13.6
*jN«rite Aflrfysia Mttood SM 4500J02
b Nitrate An«lysis'l^hMl'ivl'4^iiQ3
NOSNot Dstect below detection limit
5
-------
Reference Toxicant Data
-------
Great Lakes environmental Center
Hyalella azteca Survival
NaCl Reference Toxicant data
2002-2009
U
cs
Z.
1 4.5
~ ~ ~ ~ # *
c
C3
i-
V)
U
-J
~ ~ ~
p.
~ ~ ~ ~ ~ ¦ ~ ~
¦ B ¦ f
\ / v /
\ / v
~ ~ ~ ~
~ •:
3.5
LC50
Test Date
Mean +2 std Mean -2 std
-------
Statistical Analyses
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1,5
nitrite h azteca 96 hour acute 24 hour
Proportion
Observed
Responding
Predicted
Number
Number
Proportion
Adjusted for
Proportion
Cone.
Exposed
Resp,
Re spending
Controls
Responding
1.7700
20
0
0.0000
0 .0000
0.0151
2 . 6600
20
2
0.1000
0.1000
0.0445
4.7300
20
2
0.1000
0.1000
0.1489
8 . 0100
20
6
0.3000
0.3000
0.3307
13.5500
20
12
0.6000
0 .6000
0.5651
Chi - Square for Heterogeneity (calculated)
Chi - Square for Heterogeneity
(tabular value at 0.05 level)
2 ,314
7.815
Mu
Sigma
1.069800
0.379306
Parameter
Estimate
Std, Err.
95% Confidence Limits
Intercept
Slope
2.179586
2.636394
0 .533070
0.602559
1 .134769,
1.455379,
3.224403)
3.817409)
Theoretical Spontaneous Response Rate =
0.0000
-------
nitrite h azteca 96 hour acute 24 hour
Estimated LC/EC Values and Confidence Limits
Exposure
95% Confidence
Limit s
Point
Cone .
Lower
Upper
LC/EC
1
00
1
540
0,428
2 .593
LC/EC
5
00
2
792
1,220
4 . 033
LC/EC
10
00
3
834
2 . 094
5.197
LC/EC
15
00
4
750
2 , 965
6 .272
LC/EC
50
00
11
744
8.768
20 .424
LC/EC
85
00
29
034
17.651
97.S90
LC/EC
90
00
35
968
20.609
142.984
LC/EC
95
00
49
399
25.857
252.089
LC/EC
99
00
89
573
39.375
733-847
-------
nitrite h azteca 96 hour acute 24 hour
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10 +
9 +
8 +
7 +
6+
5+
4 +
3 +
o , . . ,o
2 +
1 +
0+ o
EC01 EC10 EC?. 5 EC50 EC75 EC90
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
nitrite hazteca 96 hour acute 48 hour
Cone.
Number
Exposed
Number
Resp.
Observed
Proportion
Responding
Proportion
Responding
Adjusted for
Controls
Predicted
Proportion
Responding
1.7700
2 .6600
4.7300
8.0100
13.5500
20
20
20
20
20
0
12
0.0000
0.1000
0.1500
0,4000
0.6000
0.0000
0.1000
0.1500
0.4000
0.6000
0.0211
0.0585
0.1807
0.3768
0.6118
Chi - Square for Heterogeneity (calculated)
Chi - Square for Heterogeneity
(tabular value at 0.05 level)
1.242
7.815
Mu
Sigma
1. 023461
0.381895
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept
Slope
2 .320045
2.618522
0 ,494795
0.570436
1.350247,
1.500468,
3.289842)
3.736576)
Theoretical Spontaneous Response Rate = 0.0000
-------
nitrite hazteca
96 hour acute 4 8
hour
Estimated LC/EC Values and
Confidence Limits
Exposure
95% Confidence
Limits
Point
Cone.
Lower
Upper
LC/EC
1. 00
1.365
0.398
2.309
LC/EC
5.00
2 .485
1 .101
3 .612
LC/EC
10 .00
3.420
1. 866
4 .655
LC/EC
15 . 00
4 .243
2 . 628
5 .601
LC/EC
50 . 00
10.555
7.987
17.127
LC/EC
85 .00
26.258
16.424
77.389
LC/EC
O
o
o
32.576
19.246
111.904
LC/EC
95 . 00
44 .837'
24.274
193,823
LC/EC
99.00
81.632
37.312
545.987
-------
nitrite hazteca 96 hour acute 48 hour
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10 +
9+
8 +
7 +
6 +
. , -O
5+
o. . . .
4+ . . .o
o ... .
3 +
2 +
1+
0+ o
- i " + - . - — — - 4. -f- - —. « — .j. „ _ _ _
EC01 EC10 EC25 EC50 EC7S EC90 EC99
-------
EPA FRQBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
nitrite h azteca 96 hour acute 72
Proportion
Observed
Responding
Predicted
Number
Number
Proportion
Adjusted for
Proportion
Cone.
Exposed
Resp.
Responding
Controls
Responding
1.7700
20
0
0.0000
0.0000
0.0232
2.6600
20
2
0.1000
0.1000
0,0803
4.7300
20
6
0.3000
0.3000
0 .2837
8.0100
20
12
0.6000
0.6000
0.5749
13.5500
20
16
0.8000
0.8000
0.8284
Chi - Square for Heterogeneity (calculated) = 0,772
Chi - Square for Heterogeneity
{tabular value at 0.05 level) = 7.815
Mu = 0.846836
Sigma = 0.300740
Parameter Estimate Std. Err. 95% Confidence Limits
Intercept 2.184161 0.479503 ( 1.244336, 3.123986)
Slope 3.325127 0,581384 ( 2.185614, 4.464641)
Theoretical Spontaneous Response Rate = 0.0000
-------
nitrite h azteca 96 hour acute 72
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit.
10+
9+
8 +
7 +
6 + . , ,
. , . . O
5 +
o
3 +
2 +
1 +
0+ O
- +
EC01
j -} ^ - - 4. ^ -f-
EC10 EC25 EC50 EC75 EC9Q EC9 9
-------
EPA FRGBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
Nitrite H azteca 96 hour acute
Proportion
Observed
Responding
Predicted
Number
Number
Proportion
Adjusted for
Proportion
Cone.
Exposed
Resp.
Responding
Controls
Responding
1. 7700
20
0
0.0000
0.0000
0.0939
2.6600
20
7
0.3500
0.3500
0.2149
4.7300
20
10
0.5000
0.5000
0.4824
8.0100
20
14
0.7000
0.7000
0.7383
13.5500
20
18
0.9000
0.9000
0.9064
Chi - Square for Heterogeneity (calculated)
Chi - Square for Heterogeneity
(tabular value at 0.05 level)
4 .422
7 . 815
Mu
Sigma
0.689662
0.335370
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept
Slope
2.943579
2.981781
0 .378057
0.514005
2.202586,
1.974332.
3.684572)
3.989231)
Theoretical Spontaneous Response Rate =
0.0000
-------
Nitrite H azteca 96 hour acute
Estimated LC/EC Values and Confidence Limits
Exposure
95% Confidence
Limits
Point
Cone.
Lower
Upper
LC/EC
1
00
0.812
0.316
1.321
LC/EC
5
00
1 .374
0.689
1.988
LC/EC
10
00
1. 819
1.039
2.485
LC/EC
15
00
2 .198
1.364
2 .901
LC/EC
50
00
4 .894
3 ,883
6.210
LC/EC
85
00
10 .895
8 .183
17.949
LC/EC
90
o
o
13.167
9.543
23.S00
LC/EC
95
00
17.431
11.919
35.598
LC/EC
99
00
29.501
17.917
77.684
-------
Nitrite H azteca 96 hour acute
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10 +
8 +
7 +
6 +
5+ O. .
4 +
3 +
2 +
1+
0+ O
_ + + + --- + + +- + "
SC01 EC10 EC25 EC50 EC75 EC90 EC99
-------
Appendix 14: Raw Data for 96-hr Toxicity of
Nitrite on Pimephalespromelas
-------
Raw Laboratory Data
-------
_ TLlJk-.
uin.1 -
,\J m. MLi JL -XJf M. i
JL JUJVJ M.
TEST MATERIAL:
PROJECT NUMBER:.
TEST SPECIES:
NO. ORGANISMS/CHAMBER:_
1Q.
\pll ^4^
phrmA- f).\<
GLC#
PHOTOPERIOD 16:8
LIGHT INTENSITY (10-20 uE/mVsl: Ambient Day 0/
AGE/SOURCE OF ORGANISM: 0 Jjl
DILUTION WATER: t>° LM '5^ ° TEST TEMPERATURE <0C): rsS% 1" ^
Day 4/
DATE
TEST
DAY
TECH.
INITIALS
TREATMENT tEVil
J>jLklritbi»e |*C)
<95'O
-~s
d*t 1 Ik.w'
3&'Q
?)K'0
¦»—y ^
^&o
PH
f)^
K^ot
t'Ob
OO (ragJL)
%2-
s.a
M
T'3
?-3
tH
K'b
S>S~
Sp. Cond. (fdnhosJcm)
9RO
,3,3(0
3BO
<3oO
MaM
1
OU
Number Live
Jl
*1
ts
1
l«P
"1
f o
*
Qbsa n/aflorts
?Mnpetature{*£}
2>l (4?
24-4-
7„4 -?
-
'"Uflfe
W)
ana
inn
T.\
/o
H3
n.^
H.H
1M
2
ftO
Number Live
iO
<1
&
\0
\o
to
u>
i
0
Observations
Temperature CC)
mz
W.t
w-a
3A-Z
PH
7,fe0
?.fc>3
7,6*3
7.^1
DO |»qJL)
i*.\
SA
M
M
6?» I
6*0
6"!
^.3
Ifi'S
e)i#
a
fp
Number Live
\o
<1
£
\o
A
to
to
S
G
&
Obs«fvabons
33:
Te«ifKsra8»ife{'C)
3lt'o
aM
».(*
au.3
PH
im
?.fe&
7.W
7-11
DO (Bflfl/LJ
Qfo
bB
g.\
(a-3
U&
s.s
*
Number Live
\0
q
o
°l
R
(O
%
f>F7
3
q
Observations.
szt
r
TampobJre (*C)
W.n
PH
3^-fc
5M.S
pH
i m
w
n?n
1.^0
1.T6
1.1^
1/TS
nnn
DO (mg/L}
faA
SP\
c,q
uo
p;.n
5."1
fed
S'O
so
Sp. Cond. Lufnhos/em}
PO-'
<335
351
H31
Observation Kev
N - Normal
IMM - Immobile
P-Pak
GniaA^
PM - Paniculate Matter
FS - Film on Surface
F - Floater
VV. ft'. M15-4.
Mk
,&^L
s>> /ill-)
REVIEWED
UW^ulI
-------
Analytical Chemistry Data
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
GleC
Grcnl
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax:231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
, . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
?/n|3T
Mailee Garton/Jamie Saxton
m. gallon
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below)
, Alkalinity
f !
©
0
ea
Chloride
Chlorophyll a
^Hardness
2
<
1*1
X
2
'it'
i
B
¦i:
a,
H
. CO
w
- H
>4-»
a
u
' m
Nitrite
N02FW-
04-01
125 ml.
HOPE
Dechlor Control -
nitrite Df*H *-j
H2SO4
i- ;
:K"
,
X"
N02FW-
04-02
125 mL
HDPE
Dechlor Control -
nitrite dm 4
Cold
'Mi
X
N02FW-
04-03
125 mL
HDPE
100 mg/L nitrite
DM M
h2so4
t
_ ;;
N02FW-
04-04
125 mL
HDPE
60 mg/L - nitrite
t>f\M 4
h2so4
-- "¦
5vV5
1.1 ¦ : ¦ ¦ ¦
¦Si
N02fW-
04-05
125 mL
HDPE
36 mg/L - nitrite
DM M
h2so4
-
m;
ii::
?' -.
N02fW-
04-06
125 mL
HDPE
21.6mg/L- nitrite
h2so4
X •
i
Shipper:
Date/Time:
Sfnpi (as*
Receiver:
Date/Time:
Temperature;
• . - - -
Definitions
BODj = Biochemical Oxygen Demand-5 day
TDS = Total Dissolve Solids
TP = Total Phosphorus
NH3 -N = Ammonia-Nitrogen
TKN = Total Kjeldahl Nitrogen
TSS - Total Suspended Solids
NOj/NOj -N — Nitrate/Nitrite-Nitrogen
Abbreviations for other freouentlv requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
HOs -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
Page _0rl
Date: J
Contact Person: Mailee Garton/Jamie Saxton
Email Address:
Phone Number:
Collector: m- g^011
Bottle IB
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
*n
Q
O
so
Chloride
' • ./ ¦ • V • • «* -- - -
Chlorophyll a
• ¦ C v '
i; W.:
fc."
. -ca
Z
1
33
Z
; -:W
- " - -
¦
* O '
TDS j
S-
Pi
H
00
CO -
' H'
Nitrate
Nitrite
-
N02FW-
04-07
125 mL
HOPE
12.9mg/L - nitrite
4
h2so4
-
X
N02FW-
04-08
125 mL
HDPE
100 mg/L- nitrite
DAM 4
Cold
X
N02FW-
04-09
125 mL
HOPE
60 mg/L - nitrite
t)M H
Cold
X
:
N02FW-
04-10
125 mL
HDPE
36 mg/L - nitrite
DfH *4
Cold
:
i-.y
X
V
N02FW-
04-11
125 mL
HDPE
21.6 mg/L - nitrite
WH
Cold
3
X
N02FW-
04-12
125 mL
HDPE
12.9 mg/L - nitrite
PN i
Cold
X
Shipper: Date/Time: Receiver: Date/Time: Temperature:
s/ciIa"! (zsq ? 17-05
2.
Definitions
BODs = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NH) -N = Ammonia-Nitrogen TKN - Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NOj/NO: -N ~ Nilrate/Nitrite-Nitrogen
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Gr E'lvjr®
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax:231-941-2240
www.elec.com
Great Lakes Environmental Center
Facility:
, . Traverse City, MI
Location:
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N - Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY
(To be completed and submitted
RECORD
with samples)
Page
( of S>
739 Hastings St
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.com
Great Lakes Environmental Center
Facility:
T Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
fig, garton Qg
cy*——•
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
5
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-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
GleC
UjN.-a &wlrcHi*rwn!ci Ccnlov
739 Hastings St
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, Ml
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Ma.
Mailee Garton/Jamie Saxton
garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below'
Alkalinity
Q
O
co
s „
Chloride
* X iV 1 f *' !
Chlorophyll a
Hardness
Z
S3
Z
z
6
z
o
z
w
Q
H
TKN
0*
H
:
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
3 _ 3
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
*1(3.
Mailee Garton/Jamie Saxton
m. gartoif ^ —
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
©
O
oa
. , -,
Chloride
J Chlorophyll a
1
d
at
£
i:
C/3
Q
H
•as:-.
0*
-*¦»
"C
• ^1
z
N02FW-
00-03
8/13/09
125 mL
HDPE
Stock 1.5 g/L
NaN02 - Ig/L nitrite
h2so4
LaV-1 •'
X "
N02FW-
00-04
8/13/09
125 mL
HDPE
Stock 1.5 g/L
NaN02— 1 g/L nitrite
Cold
X
IS
:r r.--
^ I 'X-i'
IS
....
... .
Shipper:
Date/Time;
1,
2.
I7-.K
Receiver:
Date/Time:
s -a -oi
Temperature:
D<
BODs = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NO3/NO2 -N - Nitratt/Nitrite-Nitrogcn
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj-N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N - N itrate N itrogen SRP = Sol uble Reactive Phosphorus
-------
PROJECT#: 6112-12 Date 29/09/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 0
CALIBRATION
Concentrations i
ppm)
Natural log
Millivolts
0.5
-0.6931472
-52.9
1
0
-60.2
10
2.30258509
-117.5
25
3.21887582
-139.4
50
3.91202301
-157.0
Regression Output:
Constant
-64.797384 1
Std Err of Y Est
3.706752
R Squared
0.995297
No. of Observations
5
Degrees of Freedom
3 |
X Coefficient(s) -23.227146
Std ErrofCoef. 0,92183969
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
FHM
Con
0
water
-5,1
-2.570156
0.07652362
0.03279908
FHM
12,9mg/L
0
water
-14.4
-2.169762
0.11420477
0.07048023
FHM
100 mg/L
0
water
-10.4
-2.341975
0.09613762
0.05241308
n/a
Blank
0
n/a
7.9
-3.129846
0.04372454
0
-------
PROJECT#: 6112-12 Date 17/08/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 4
CALIBRATION
Concentrations
ppm)
Natural log
Millivolts
0.5
-0.6931472
-51.4
1
0
-61.6
10
2.30258509
-117.0
25
3.21887582
-139.6
50
3.91202301
-157.6
Regression Output:
Constant
-64.563877
Std Err of Y Est
2.739584
R Squared
0.997460
No. of Observations
5
Degrees of Freedom
3
X Coefficients) -23.383609
Std Err of Coef. 0.68131279
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
FHM
Con
4
water
-52.1
-0.533018
0.58683142
0.41038079
water
FHM
100 mg/L
4
water
-43.5
-0.900797
0.40624588
0.22979525
n/a
Blank
4
n/a
-24
-1.734714
0.17645063
0
-------
Nitrite Day 0 FHM Analytical Results 8.13,09
LabSamplelD
SampleDescription
ParamID
Results
Units
RL LabQu Analysisl AnalysisDate
Sample Date
Analyst
Calibration Date
Calibration
HB08130272
Dechior Control-nitrite
N02
<0,0036
mg/L
0.016425 U
A4500B
14/08/2009
13/08/2009
J!
8/
4/2009
Jl
HB08130272A
Dechtor Control-nitrite
N03
1,021
mg/L
0.08854
A4500F
13/08/2009
13/08/2009
Ben Cook
8/
3/2009
Ben Cook
HB08130272B
Dechior Control-nitrite
N03
1.005
mg/L
0.08854
A4500F
13/08/2009
13/08/2009
Ben Cook
8/
3/2009
Ben Cook
HB08130273
1OOmg/L-nitrite
N02
104
mg/L
16.425
A4500B
14/08/2009
13/08/2009
Jl
8/
4/2009
Jl
HB08130273
1QOmg/L-nitrite
N03
<2.7
mg/L
11.0675 U
A4500F
13/08/2009
13/08/2009
Ben Cook
8/
3/2009
Ben Cook
HB08130274
60mg/L-nitrite
N03
1.5
mg/L
5.53375 J
A4500F
13/08/2009
13/08/2009
Ben Cook
8/
3/2009
Ben Cook
HB08130274A 60mg/L-nitrite
N02
59
mg/L
16.425
A4500B
14/08/2009
13/08/2009
Jl
8/
4/2009
Jl
HB08130274B
60mg/L-nitrite
N02
60
mg/L
16.425
A4500B
14/08/2009
13/08/2009
Jl
8/
4/2009
Jl
HB08130275
36mg/L-nitrite
N02
35
mg/L
16.425
A4500B
14/08/2009
13/08/2009
Jl
8/
4/2009
Jl
HB08130275
36mg/L-nitrite
N03
<1.1
mg/L
4.427 U
A4500F
13/08/2009
13/08/2009
Ben Cook
8/
3/2009
Ben Cook
HB08130276
21.6mg/L-nitrite
N02
17
mg/L
16.425
A4500B
14/08/2009
13/08/2009
Jl
8/
4/2009
Jl
HB08130276
21.6mg/L-nitrite
N03
2.3
mg/L
2.2135
A4500F
13/08/2009
13/08/2009
Ben Cook
8/
3/2009
Ben Cook
HB08130277
12Jmg/L-nitrite
N02
12.8
mg/L
1.6425
A4500B
14/08/2009
13/08/2009
Jl
8/
4/2009
Jl
HB08130277
12.9mg/l-nitrite
N03
<0.5
mg/L
2.2135 U
A4500F
13/08/2009
13/08/2009
Ben Cook
8/
3/2009
Ben Cook
HB08130278
Stock 1,5g/L NaN02-1g/L nitrite
N02
1450
mg/L
1642.5 J
A4500B
14/08/2009
13/08/2009
Jl
8/
4/2009
Jl
HB08130278
Stock 1.5g/L NaN02-1g/L nitrite
N03
<27
mg/L
110.675 U
A45Q0F
13/08/2009
13/08/2009
Ben Cook
8/
3/2009
Ben Cook
Page 1
-------
Nitrite Day 4 FHM Analytical Results 8.17,09
LabSamptelD
SampleDescription
ParamiD
Results
Units
RL
LabQualifier
AnalysisMethod
AnalysisDale
SampleDatc Analyst Calibration Date
Calibration Person
HB08170296
Dechlor Control-nitrite-Day 4
N03
1.148
mg/L
0.08854
A4500F
17/08/2009
17/08/2009 Ben Cook 8/17/2009
Ben Cook
HB08170296
Dechlor Control-nitrite-Day 4
N02
0.0187
mg/L
0.016425
A4500B
19/08/2009
17/08/2009 Jl 8/19/2009
Jl
HBQ8170297
100mg/L-nitrife
N03
<1.6
mg/L
6.6405
U
A4500F
17/08/2009
17/08/2009 Ben Cook 8/17/2009
Ben Cook
HB08170297
100mg/L-nitrite
N02
111
mg/L
16.425
A4500B
19/08/2009
17/08/2009 Jl 8/19/2009
Jl
HB08170298
60mg/L-nitrite
N03
21.6
mg/L
4.427
A4500F
17/08/2009 17/08/2009 Ben Cook 8/17/2009
Ben Cook
HB08170298
60mg/L-nitrite
N02
57
mg/L
16.425
A4500B
19/08/2009
17/08/2009 Jl 8/19/2009
Jl
HB08170299
36mg/L-nitrite
N03
<0.5
mg/L
2.2135
U
A4500F
17/08/2009
17/08/2009 Ben Cook 8/17/2009
Ben Cook
HB08170299
36mg/L-nitrite
N02
35
mg/L
16.425
A4500B
19/08/2009
17/08/2009 Jl 8/19/2009
Jl
HB08170300
21.6mg/L-nitrite
N03
<0.5
mg/L
2.2135
u
A4500F
17/08/2009
17/08/2009 Ben Cook 8/17/2009
Ben Cook
HB08170300
21.6mg/L-nitrite
N02
21
mg/L
16.425
A4500B
19/08/2009
17/08/2009 Jl 8/19/2009
Jl
HB08170301
12.9mg/L-nitrite
N03
<0.5
mg/L
2.2135
u
A4500F
17/08/2009
17/08/2009 Ben Cook 8/17/2009
Ben Cook
HB08170301A
12.9mg/L-nitrite
N02
13.8
mg/L
1.6425
A4500B
19/08/2009
17/08/2009 Jl 8/19/2009
Jl
HB08170301B
12.9mg/L-nitrite
N02
14.2
mg/L
1.6425
A4500B
19/08/2009
17/08/2009 Jl 8/19/2009
Jl
Page 1
-------
Reference Toxicant Data
-------
Fathead Minnow Survival
Sodium Chloride (NaCl) Toxicity Data
2007-2009
10
u
u
h4
6
5
#° ^ ,*VW / ^ ^%° ^ ^ VV /
H 48 hour LC50 Mean plus 2 S.D. I Mean minus 2 S.D.
-------
Statistical Analyses
-------
TRIMMED SPEARMAN-KARBER METHOD. MONTANA STATE UNIV
FOR REFERENCE, CITE;
HAMILTON, M.A., R.C. RUSSO, AND R.V. THURSTON, 1977.
TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING MEDIAN
LETHAL CONCENTRATIONS IN TOXICITY 3I0ASSAYS.
ENVIRON. SCI. TECHNOL. 11 (7) ; 714-719;
CORRECTION 12 (4) :417 (1978) .
DATE: 8/13/09 TEST NUMBER; 6112-12
CHEMICAL: NITRITR SPECIES: FHM
RAW DATAi
CONCENTRATION(MG/L
NUMBER EXPOSED;
13.50 19.00 35.00 58.70 107.50
20 20 20 20 20
DURATION (HOURS
EC50 LOWER 95% LIMIT UPPER 95% LIMIT PERCENT
TRIM
72
87.87
63 ,47
121.63
40.00
12 0.44- wx. XUjd
63\ ?0
•d"0"l i'
35.00
-------
EPA PROEIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
nitrite fhm acute 96 hour
Proportion
Observed
Responding
Predicted
Number
Number
Proportion
Adjusted for
Proportion
Cone.
Exposed
Resp.
Responding
Controls
Responding
Control
20
1
0.0500
0.0000
0.0880
13.5000
20
3
0.1500
0.0680
0.0001
19.0000
20
2
0.1000
0.0131
0.0015
35.0000
20
1
0.0500
-.0417
0.0367
58.7000
20
7
¦ 0.3500
0.2873
0.2135
107.5000
20
13
0.6500
0.6162
0.6445
Chi - Square for Heterogeneity (calculated)
Chi - Square for Heterogeneity
(tabular value at 0.05 level)
2.445
7 . 815
Mu
Sigma
1.947848
0.225583
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept
Slope
-3 . 634746
4.432966
3.125155
1.S34449
-9.760050,
1.229445,
2.490558}
7.536487)
Spontaneous 0.088014
Response Rate
0.036271
0.016922,
0.159105)
-------
nitrite fhm acute 96 hour
Estimated LC/EC Values and Confidence Limits
Exposure 95% Confidence Limits
Point Cone. Lower Upper
LC/EC
1. 00
26 .490
1
249
44.700
LC/EC
5 . 00
37.739
4
404
55.776
LC/EC
h4
O
O
O
45.577
8
563
63.197
LC/EC
15.00
51.767
13
335
69.162
LC/EC
50.00
88.685
64
587
135.965
LC/EC
85 . 00
151.930
110
477
756.833
LC/EC
O
o
o
ON
172.566
120
391
1183.649
LC/EC
95.00
208.406
135
946
2309.540
LC/EC
99 . 00
296.908
169
158
8167.011
-------
nitrite fhm acute 96 hour
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10 +
9+
8 +
7 +
6+
5+
4 -¥
-o
-o
3 +
2 +
1 +
0+ o
EC01 EC10 3C2 5 ECSO EC75 EC90
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
nitrite fhm acute 96 hr ec50
Proportion
Observed
Responding
Predicted
Number
Number
Proportion
Adjusted for
Proportion
Cone.
Exposed
Resp.
Responding
Controls
Responding
Control
20
1
0.0500
0.0000
0.0837
13.5000
20
3
0.1500
0.0724
0.0092
19.0000
20
2
0.1000
0.0178
0.0281
35.0000
20
3
0.1500
0.0724
0.1333
58.7000
20
9
0.4500
0.3998
0 .3318
107.5000
20
13
0.6500
0.6180
0.6391
Chi - Square for Heterogeneity (calculated)
Chi - Square for Heterogeneity
(tabular value at 0.05 level)
1.561
7.815
Mu
Sigma
1, 913119
0.332192
Parameter
Estimate
Std. Err.
95% Confidence Limits
Intercept
Slope
¦0.759075
3.010308
2 .033676
1 .110423
-4 .74508.0,
0.833877,
3.226930)
5.186738)
Spontaneous
Response Rate
0 . 083682
0.046328
-0.007121,
0.174484)
-------
nitrite fhm acute 96 hr ec50
Estimated LC/EC Values and Confidence Limits
Exposure 95% Confidence Limits
Point Cone, Lower Upper
LC/EC
1
00
13
815
0 .156
29.495
LC/EC
5
00
23
265
1, 007
40.698
LC/EC
10
00
30
717
2 ,694
48 ,737
LC/EC
15
00
37
054
5 .195
55 .455
LC/EC
50
00
81
869
54.183
147.354
LC/EC
85
00
180
885
115.054
1923.157
lc/ec
90
00
218
201
130.165
3730 .302
LC/EC
95
00
288
099
155.171
10026.474
LC/EC
99
00
485
173
213.324
64779.715
-------
nitrite fhm acute 96 hr ec50
PLOT OP ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Prefoit
10+
9+
¦ O.
7+
6 +
5 +
4 +
-o
o
3 +
2 +
1 +
0 +
— -I*-"-" — — — — — — — — — — — — — — — _ — —— ^ — — — — — — — — —
EC01 ECI0 EC25 ECSO EC75 EC90 EC99
-------
Appendix 15: Raw Data for 32-day Toxicity of
Nitrite on Pimephalespromelas
-------
Raw Laboratory Data
-------
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
Great Lafcs. Enwfronuteniel Ctsiter
TEST MATERIAL:
• (NjftUlk TYPE OF TEST: M OuO -HwlVl 1 a K QgivljiK DILUTION WATER: JVgKb»r
f/,1 li-ia 3«/ c.*l ¦>* eu . .
PROJECT NUMBER: io\ 13~1 ^ NUMBER OF FISH/CHAMBER: ^5 2~C DILUTOR NUMBER: B>\ i t 6 j PHQTOPERIOD: |(p
TEST SPECIES:
NUMBER OF CHAMBERS: M . TEST TEMPERATURE: 3G>£. j'1- AGE OF Embryos: O M
DATE
TIME
TEST
TIME
(DAYS)
TECH.
1NIT.
TREATMENT
REPLICATE NUMBER
ft Co-23
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Number Live Eggs
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Number Hatched Larvae
Observations
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13
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12-Zdz'.
flhsirvntlrms-
Obseivation Kay: DOB - Dried Out on Chamber PM - Particulate Matter
Dated: Reviewed
ERR - Erratic Swimming
¦2k
FS - Film on Surface
F - Floater
IMM • Immobile FU - Fungus
-------
3rcst E-rviroomenia^ Cesuer
TEST MATERIAL:. KKVujct
PROJECT NUMBER:_
TEST SPECIES;
Pfrrn
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
TYPE OF TEST.
NUMBER OF FIStVCHAMBER:
NUMBER OF CHAMBERS:
:H/f!H&MBFR- 3£>
DILUTION WATER;
: Ck-g-MLJS
DILUTOR NUMBER:
G&Jl
...
PHOTOPERIOD: U/9
TEST TEMPERATURE: D*5— I* AGE OF Embryos:_
DATE
TEST
TIME
(DAYS)
TECH.
INIT.
TREATMENT
ft Va.-Sn^^
TIME
REPLICATE NUMBER
1
2
3
4
1
2
3
4
1
2
3
4
Bo
P©
SO
O-O
ao
Number Dead Fish
Observations
3
/
Number Live Fish
Number Dead Fish
Observations
/
Number Live Fish
Number Dead Fish
Observations
•BlrV
10
Number Live Fish
So
30
30
3D
5C>
0>O
\°\
Q£>
90
Number Dead Fish
1
Observations
t/l&
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Number Live Fish
Number Dead Fish
Observations
"X
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Number Live Fish
'36
70
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90
20
30
11
££>
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Number Dead Fish
Observations
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Number Dead Fish
V
Observations
Observation Key. DOB - Dried Out on Chamber PfJk - Particulate Matter ERR - Erratic Swimming a FS - Film on Surface F - Fbater IWM - Immobile FJJ - Fungus
Dated: Reviewed by^_
bRH - fcrratic swimming a fs - riirn on ;
(J
-------
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
Great Idles. Environmental Center
TEST MATERIAL:
PROJECT NUMBER:_
TEST SPECIES:
uire--ra-
FHtV\
TYPE OF TEST:
NUMBER OF FISWCHAMBER:
NUMBER OF CHAMBERS:
SH/CHAMBER: g? u
DILUTION WATER:
WcVilor
DILUTOR NUMBER:
To I IaJL-.
PHOTOP6RIOD:
Ttjrg;
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TEST TEMPERATURE
AGE OF Embryos:_
DATE
TEST
TIME
(DAYS)
TECH.
IN1T.
TREATMENT
e.ovv
G
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TIME
REPLICATE NUMBER
i
2
3
4
1
2
3
4
1
2
3
4
h\d\
Number Live Fish
w
20
w
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7?0
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Number Dead Fish
Observations
tP
\5
/
Number Live Fish
Number Dead Fish
Observations
iu
/
Number Live Fish
Number Dead Fish
Observations
-------
Grv.i Lakes Envfe-oflmcmal Center
L:
TEST MATERIAL:
PROJECT NUMBER;_ uwa-r»
TEST SPECIES: r hh
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
j)gcV;io/'
TYPE OF TEST
NUMBER OF FISH/CHAMBER
NUMBER OF CHAMBERS:
30 DILUTOH NUMBER: felUC.
DILUTION WATER:
PHOTOPERIOD:
\3 ' 8
4-
TEST TEMPERATURE: <3*5 ~ ) ° C- AGE OF Embryos:,
DATE
TEST
TIME
(DAYS)
TECH,
INIT.
TREATMENT
CorvKo 1
/ L
5 ^3 /L-
TIME
REPLICATE NUMBER
t
2
3
4
1
2
3
4
1
2
3 .v
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4
¦VI
£6
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Number Live Fish
I*
50
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Number Dead Fish
Observations
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Number Live Fish
26
30
fb
$
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5-0
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Number Dead Fish
a
Observations
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-------
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
Great Lakss Environments? Cc^lcr
: Hihxh feoA" TYPE OP TEST:.
NUMBER OF FISH/CHAMBER: J PiSk U)
TEST MATERIAL:
PROJECT NUMBER:
TEST SPECIES:
DILUTION WATER:
NUMBER OF CHAMBERS:
JL
DILUTOR NUMBER:
PHOTOPERIOD:
Ito He
TEST TEMPERATURE: t I AGE OF Embryos:
DATE
TIME
TEST
TIME
(DAYS)
TECH,
INIT.
TREATMENT
REPLICATE NUMBER
C. 3*5^^
D 5"C^3lt_
e 100*31
0
Number Live Eggs
J[2.
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30
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Observation Key:
Dated:
DOB - Dried Out on Chamber
PM - Particulate Matter
ERR - Erratic Swimming
Reviewed fay: f f PVTI
9
FS • Film on Surface
F - Floater
IMM - Immobile FU • Fungus
-------
Jrrcrt Lakes ErrvfranmerKsl Center
TEST MATERIAL:
PROJECT
TEST SPECIES:
NvV^fcj
NUMBER: ^
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
TYPE OF TEST:
NUMBER OF FISH/CHAMBER:.
NUMBER OF CHAMBERS:
SH/CHAMBER: "
DILUTION WATER:
DILUTOR NUMBER:
: ftl
Q* e|jA
W ¦ JKTmi ¦
-
PHOTOPERIOD;
ikll.
TEST TEMPERATURE:
AGE OF Embryos:_
DATE
TEST
TIME
(DAYS)
TECH,
INIT.
TREATMENT
C.
rim,
E u\.Y-qiiu
TIME
REPLICATE NUMBER
1
2
3
4
1
2
3
4
t
2
3
4
Number Live Fish
Po
Bo
1*1
1 n
5"
16
5"
Number Dead Fish
Observations
T
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Number Live Fish
Number Dead Fish
Observations
nWY
/
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Number Dead Fish
Observations
ro
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2-
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3
\2
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$
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Number Dead Fish
4
5
3-
Observations
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/
Number Live Fish
Number Dead Rsh
Observations
Observation Key:
Dated:
DOB - Dried Out on Chamber
PM - Particulate Matter
Reviewed bf.
ERR - Erratic Swimming
FS - Rim on Surface
F - Floater
1MM - Immobile
FU - Fungus
-------
GtfC
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
Gfcs? Lafces Eiwfronnwfvtai Center
TEST MATERIAL:
PROJECT NUMBER:_
TEST SPECIES:
wtibm
TYPE OF TEST?
NUMBER OF FISH/CHAMBER:
NUMBER OF CHAMBERS;
4^0.1 QjJyhtfohu. I
SH/CHAMBER: " DILI
UTION WATER:
DILUTOR NUMBER:
(A
TEST TEMPERATURE:
12
PHOTOPERIOD
.AGE OF Embryos:_
DATE
TEST
TIME
(DAYS)
TECH.
INIT.
TREATMENT
9 S *~vs£) j
IOO
TIME
REPLICATE NUMBER
1
2
3
4
1
2
3
4
1
2
3
4
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14
€13
Number Live Fish
aD
A
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11*
3
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Number Dead Fish
*4
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9*
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<6p>
/
Number Live Fish
Number Dead Fish
Observations
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/
Number Live Fish
Number Dead Fish
Observations
n
Number Live Fish
£? 0
-------
GleC
Great Lshcs Environmental Corner
TEST MATERIAL:
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
TYPE OF TEST:
PROJECT NUMBER:
FyVH
k 4 DILUTION WATER:_
chip"
TEST SPECIES:
NUMBER OF FISH/CHAMBER:.
NUMBER OF CHAMBERS:
30
DILUTOR NUMBER:
u
PHOTOPERIOD:
19'' ft
TEST TEMPERATURE:
Sfp-t- \°C
AGE OF Embryos:
DATE
TEST
TIME
(DAYS)
TECH.
INIT.
TREATMENT
2% /L-
50 tv\9 H
)d0
TIME
REPLICATE NUMBER
1
2
3
4
i
2
3
4
i
2
3 ju-
i
4
y\
%*=>
Number Live Fish
IP>
w
|0
"I
0
0
o
I
Number Dead Fish
V
Observations
1/3-
^Vv
Number Live Fish
10
1
0
0
o
1
Number Dead Fish
a
Observations
q/t|
$s>
Number Live Fish
<7
10
1
*3*
li
(1
0
f]
0
Number Dead Fish
1
i
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I
Observations
Ml
'3l
/
Number Live Fish
Number Dead Fish
Observations
"lis
CR
ps>
vn
Number Live Fish
n
a
I \
o
o
o
o
Number Dead Fish
Observations
Number Live Fish
Number Dead Fish
Observations
Number Live Fish
Number Dead Fish
Observations
f'
¦ '
Observation Key: DOB - Dried Out on Chamber PM - Particulate Matter
Dated: Reviewed by;<
ERR - Erratic Swimmifia FS - Filr
iim on Surface
F - Floater
IMM - Immobile FU - Fungus
-------
32-Day Early Life-StageToxicity Test Fish Weight Data for
HECD B-12 Nitrite
Plmephales promelas (Fathead Minnow)
Nominal (and Measured)
Test Concentration
A
Weight of
Pan and Fry
(g)
B
Weight of
Oven-Dried
Pan
A-B
Total Dry
Weight (g)
C
Number of
Fry Weight
a-b/c
Average Dry
Fry Weight
(mg)
A-B/20
Biomass
Weight
(mg)
Dilution
Water/Control
rep 1
0.91240
0.79792
0.11448
20
5.724
5.724
rep2
0.91823
0.79274
0.12549
19
6.605
6.275
rep3
0.90187
0.78726
0.11461
17
6.742
5.731
rep4
0.92132
0.79470
0.12662
16
7.914
6.331
6.25 (5.53) mg/L
rep 1
0.92224
0.78506
0.13718
16
8.574
6.859
rep2
0.90569
0.79197
0.11372
17
6,689
5.686
rep3
0.90632
0.79101
0.11531
18
6.406
5.766
rep4
0.90433
0.78188
0.12245
20
6.123
6.123
12.5(11.3) mg/L
rep 1
0.92341
0.79630
0.12711
19
6.690
6.356
rep2
0.92566
0.78480
0.14086
12
11.738
7.043
rep3
0.91563
0.79041
0.12522
19
6.591
6.261
rep4
0.92263
0.79698
0.12565
19
6,613
6,283
25.0 (22.5) mg/L
rep 1
0.92832
0.79739
0.13093
16
8,183
6.547
rep2
0.92122
0.79554
0.12568
16
7.855
6.284
rep3
0.90215
0.79192
0.11023
19
5.802
5.512
rep4
0.91586
078907
0.12679
12
10.566
6.340
50,0 (49.2) ma/L
rep 1
0.85500
0,78959
0.06541
9
7.268
3.271
rep2
0.81383
0.79627
0.01756
2
8.780
0.878
rep3
0.85950
0.79093
0.06857
9
7.619
3.429
rep4
0.87818
0.79378
0,08440
11
7.673
4.220
-------
GleC
O > 1
CHRONIC TOXICITY TEST- WEIGHT DATA
Oeat'Lakes Envl-otimental Center
TEST MATERIAL: Ai'i-hi
TEST NUMBER; blU-1 >
TECHNITIAN'S INITIALS:
QC INITIALS:
WEIGHT DATE: ^ j q
TEST DATE:
DRYING DURATION (HOURS): 24
TYPE/MODEL OF DRYING OVEN: Blue M
OVEN TEMPERATURE fC): 60
TEST SPECIES: PHM
Treatment
Level
Rep.. I
A
Weight of
Pan and Fry
(r)
B
Weight of
Oven-Dried
Pans (g)
A-B
Total Dry
Fry Weight
(s)
c
Number of
Fry Weight
A-B/C
Average Dry
Fry Weight
(mg)
Biomass
weight
(mS)
r-\ STL fTTT\
piWfum
CjfV\ttM
1
D.Aiauo
r>. nicrt
ft, 11441?
2o
5,134
5,15m
2
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n.iasw
fllj.UOS
&.7J1S
3
11
f, i4a
5.131
4
0/ 3213 £
6,7^490
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-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrite
Dilution Water ControIRe
p1
Fish Number
Rep 1
Rep 2
Rep 3
Rep 4
1
0.03478
0.06858
0.04277
0.05974
2
0.04611
0.06755
0.00674
0.01069
3
0.05525
0.02566
0.04898
0.02100
4
0.04660
0.00665
0.07382
0.04591
5
0.04949
0.03985
0.03839
0.07510
6
0.02005
0.00265
0.04805
0.03741
7
0.03717
0.02444
0.04069
0.00865
8
0.01846
0.05731
0.04399
0.04575
9
0.04397
0.03347
0.04967
0,03279
10
0.01932
0.00338
0.02720
0.04116
11
0.01288
0.00366
0.08130
0.06718
12
0.06640
0.04356
0.03928
0.06290
13
0.02149
0.06721
0.02194
0.06381
14
0.04497
0.04360
0.01391
0.03884
15
0.04237
0.06174
0.01604
0.02297
16
0.04338
0.04082
0.02717
0.00436
17
0.02598
0.03889
0.00760
18
0.02287
0.01350
19
0.01545
0.02736
20
0.03601
N
20
19
17
16
Min
0.01288
0.00265
0.00674
0.00436
Max
0.06640
0.06858
0.08130
0.07510
Mean
0.03515
0.03526
0.03691
0.03989
-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrite
6.25 rng/L Nitrite
Fish Number
Rep 1
Rep 2
Rep 3
Rep 4
1
0.03746
0.05904
0.05429
0.07465
2
0.06892
0.03243
0.06500
0.08642
3
0.06617
0.06276
0.05812
0.06998
4
0.09318
0.04334
0.04892
0.05711
5
0.04044
0.02029
0.05899
0.05654
6
0.06361
0.04373
0.04634
0.06090
7
0.11061
0.04827
0.06671
0.03460
8
0.03000
0.02982
0.04616
0.04000
9
0.03274
0.06074
0.03380
0.04042
10
0.01794
0.04934
0.02245
0.07554
11
0.03001
0.03889
0.03525
0.04395
12
0.03954
0.02929
0.01238
0.01382
13
0.03960
0.03060
0.01369
0.01037
14
0.00578
0.01249
0.02700
0.00349
15
0.01066
0.00838
0.00519
0.00316
16
0.00646
0.00405
0.00540
0.00483
17
0.00175
0.00725
0.00270
18
0.00180
0.00242
19
0.00194
20
0.00239
N
16
17
18
20
Min
0.00578
0.00175
0.00180
0.00194
Max
0.11061
0.06276
0.06671
0.08642
Mean
0.04332
0.03384
0.03382
0.03426
-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrite
12.5 mg/L Nitrite
Fish Number
Rep1
Rep 2
Rep 3
Rep 4
1
0.05424
0.07595
0.00893
0.03861
2
0.03588
0.00372
0.02091
0.05937
3
0.03070
0.01472
0.03030
0.03077
4
0.00218
0.05102
0.02845
0.02060
5
0.00212
0.05959
0.03323
0.07007
6
0.00304
0.06063
0.04106
0.02057
7
0.05099
0.04891
0.04502
0.03280
8
0.07428
0.07333
0.02699
0.04035
9
0.05877
0.04260
0.05137
0.02585
10
0.00434
0.09933
0.03713
0.01130
11
0.01287
0.09061
0,04557
0.05171
12
0.00692
0.10942
0.02475
0.03001
13
0.00581
0.02214
0.02603
14
0.10232
0.05384
0.02718
15
0.01640
0.02500
0.03831
16
0.04689
0.02660
0,02183
17
0.06647
0.01190
0.04455
18
0.04829
0.06712
0.01882
19
0.03444
0.04403
0.04844
20
N
19
12
19
19
Min
0.00212
0.00372
0.00893
0.01130
Max
0.10232
0.10942
0.06712
0,07007
Mean
0.03458
0.06082
0.03391
0.03459
-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrite
25,0 mail Nitrite
Fish Number
Repi
Rep 2
Rep 3
Rep 4
1
0.05820
0.00288
0.00580
0.05233
2
0,02820
0,05194
0.01996
0.07372
3
0.07919
0.04764
0.06731
0.08279
4
0.04780
0,04481
0.05801
0.01413
5
0.04472
0.05725
0.00276
0.11343
6
0.04051
0.00444
0.04750
0.02724
7
0.03288
0,03000
0.05366
0.00826
8
0.05565
0.06106
0.05561
0.00703
9
0.02840
0.03826
0.00481
0.08119
10
0,01036
0,03052
0.00548
0.05305
11
0.06148
0.05456
0.07339
0.03940
12
0.05487
0,03544
0.00660
0.09357
13
0.06862
0.02813
0.06482
14
0.05464
0.03571
0.00314
15
0,00085
0.06343
0.00290
16
0.00094
0.05310
0.04753
17
0.03093
18
0.00344
19
0.03126
20
N
16
16
19
12
Min
0.00085
0,00288
0.00276
0.00703
Max
0.07919
0.06343
0.07339
0.11343
Mean
0.04171
0,03995
0.03078
0.05385
-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrite
50.0 mg/L Nitrite
Fish Number
Rep 1
Rep 2
Rep 3
Rep 4
1
0.04068
0.01495
0.02021
0.01979
2
0.02318
0.08710
0.05310
0.08678
3
0.06674
0.03246
0.01699
4
0.02214
0.05896
0.08604
5
0.03072
0.03666
0.00374
6
0.04289
0.01839
0.07741
7
0.02931
0.04359
0.01661
8
0.02657
0.05062
0.00345
9
0.05145
0.04970
0.04300
10
0.03621
11
0.04177
12
13
14
15
16
17
18
19
20
N
9
2
9
11
Mill
0.02214
0.01495
0.01839
0.00345
Max
0.06674
0.08710
0.05896
0.08678
Mean
0.03708
0.05103
0.04041
0.03925
-------
Pimepales promelas (Fathead Minnow)
Wet weight for the 32-Day Toxicity Test
HECD B-12 Nitrite
100.0 mg
11 Nitrate
Fish Number
Rep 1
Rep 2
Rep 3
Rep 4
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
N
0
0
0
0
Min
Max
Mean
-------
N»ki-k
Day 32 BororrFHM wet weight 9/8/09
Fish Control Control Control Control
Number Rep 1 Rep 2 Rep 3 Rep 4
1
fi.034T¥
0, 0W52
o.owi
2
0 ..ifeT %
Moi/IH
3
(V05S;3
0-oqw
o. oa ioo
4
n.oHW/o
O.OHtS
(UY?3»
5
o.m&s
0. o?M
ftiVlSlO
6
O-OQooS
cU(j2£5»
0-O^Yds
0-65111
7
o.onn
0.02-Iff
8
n.olwy
Ot 0 *>?31
A.0M3W
9
n.ow)
o,^y)u
0-h^in
rt ,53371
10
0-01^32
O.OayhS
M-mo
fi.ftMllto
11
A. for 13 o
0«om
12
ivnuiwo
u, 0H36U
o.bu^A
13
5.0;^ m
o. quia*
iu&iw
fi. tty!#I
14
o-o4W
OOTbO
O.OM^
15
VOH^I
0-
O.GItoOM
yam
16
lumn
5,60414
17
0-03W
n.0Q>n
18
iiomi
0.0U5D
19
5.
20
O-O^oi
Fish 12,5 mg/L 12.5mg/L 12.5 mg/L 12.5 mg/L
Number Rep 1 Rep 2 Rep 3 Rep 4
1
Q.CXmiH
(3,0? S4S
0,00115
o.o my
2
0.c^5M
0.00512
O.OStVT
3
0.0 307 <3
0,0(Nil
0.0 W
6 0^877
4
ot oS !0i
0.0 4*5
O.Q lobd
5
0, GO2rt.
0. n7 ®<-7
6
0.0 ©lof
d.om'b
a^Hlsb
0,4*o57
7
a.0H6l\
n.0V5o2
o.oWo
8
0 Q^llb
a.QUQmi$
fl.O^a
7
(Utoui
WW
0,0'^i
d, OSMt o
8
n.CBOOa
n, 091^ >
OttMlrib
(1. a^oao
9
6. miiitsMort
G. OSSFb
10
i o!-m
jUW
o-o?ssv
11
o.ixm\
12
(St 0?^
0, WW
0.OP38
(VhlW
13
n. bs%o
ft. 030VO
0.01169
6. til (B7
14
{), aos ir
Q.WWl
0,06344
15
O.aoM
rt.oos i")
O.dOSly?
16
fl.OOHb
0.OOMC5
6M&U ()
0.0Mft3
17
1% OOllfT
O.ODIse
OiOoaio
18
o.ooi^o
().floW
19
20
<5-00^1
Fish 25.0 mg/L 25.0 mg/L 25.0 mg/L 25.0 mg/L
Number Rep 1 Rep 2 Rep 3 Rep 4
1
0,0GUO
O.ooaM
&.PO<5 Bo
O.D5a?1
2
lOQ-%30
0.051^4
D'0\q<\ y
O.blin
3
n
0,kSTA%
o, ooaiyj
o.nwz
6
fi, OHtjc;,
o.ootmn-
t)4t!«50
f-oa 7j4-
7
h,Man
8>q3o®d
O'OSHfU
o.oofiatp
8
5*582u>
0.0049!
10
dMo52
O.0054G
11
O.DIO:^
O'OUM
0.03^0
12
D.DS4F1
0,£>iKpto0
<7^367
13
o.D^ei i
O.DU4S3
14
G.OSM
6'0W1\
15
o-oy^H?
O.
20
-------
Day 32-Boron FHM wet weight 9/8/09
Fish 50 mg/L 50 mg/L 50 mg/L 50 mg/L
Number Rep 1 Rep 2 Rep 3 Rep 4
1
O.OHDli#
o-omt
6.03L03L\
o. mm
2
0.i>33i6
0-O&7ID
0.o%3\d
0-00 (pi &
3
o.OiptoTf
C-OSWlt
0.01
4
fi'OStpO1!
5
O.OlyW
0.QOVW
6
o Mm
O.OlbW
Mrrtl
7
o.mnu
O'OMW
HAW-
8
OOibtl
c.oiobd-
9
0^6 IH6
o.&Wio
0.0^300
10
11
0.0M 111
12
13
14
15
16
17
18
19
20
-------
Analytical Chemistry Data
-------
Nitrite Day -2 FHM 32 Day Analytical Results 8.3.09
LabSamplelD
SampleDescription
ParamlO
Results Units
RL LabQualifier
AnalysisMethod
AnafysisDate
SampfeDate Analyst Calibration Date
Calibration Person
H808030137
Water Control Tank #1
N02
0.0128
mg/L
0.016425 J
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HBG8Q30137A
Water Control Tank #1
N03
1.187
mg/L
0.08854
A45G0F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
HBQ8030137B
Water Control Tank #1
N03
1.160
mg/L
0.08854
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
KB08030138
Water Control Tank #2
N03
1.215
mg/L
0.08854
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
KBD8030138
Water Control Tank #2
N02
0.030
mg/L
0.016425
A45GQB
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030139
Water Control Tank #3
N03
1.237
mg/L
0.08854
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
HB0BO3O139
Water Control Tank #3
N02
<0.0031
) mg/L
0.016425 U
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
J8
HB06030140
Water Control Tank #4
N03
1.160
mg/L
0,08854
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
HS08030140
Water Control Tank #4
N02
0.0329
mg/L
0.016425
A4500B
03/08/2009
03/08/2009 JB 6/3/2009
JS
H8DB030141
Water 6.25rng/L nitrite Tank #1
N03
2.1
mg/L
2.2135 J
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
HBO6030141
Water 6.25mg/L nitrite Tank #1
N02
5.01
mg/L
0.82125
A4S008
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030142
Water 6.25mg/L nitrite Tank #2
N03
1.6
mg/L
2.2135 J
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Code
HB06030142
Water 6.2 5m g/L nitrite Tank #2
N02
5.01
mg/L
0.82125
A450GB
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB06030143
Water 6.25mg/L nitrite Tank #3
N03
1.5
mg/L
2.2135 J
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
HB08030143
Water 6.25mg/L nitrite Tank #3
N02
5.11
mg/L
0.82125
A4500B
03/08/2009
03/08/2009 JB 6/3/2009
JB
HB 08030144
Water 6.25mg/L nitrite Tank #4
N03
3.5
mg/L
2.2135
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
HB06030144A
Water 6.2Smg/L nitrite Tank #4
N02
4.93
mg/L
0.82125
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030144B
Water 6.25mg/L nitrite Tank #4
N02
5.06
mg/L
0.82125
A4500B
03/08/2009
03/08/2009 JB 6/3/2009
JB
HB08030145
Water 12.5mg/L nitrite Tank #1
N03
3.2
mg/L
4.427 J
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
HB06030145
Water 12.5mg/L nitrite Tank #1
NG2
10.4
mg/L
1,6425
A4500B
03/08/2009
03/08/2009 JB 6/3/2009
JB
HB08030146
Water 12.5rng/L nitrite Tank #2
N03
1.4
mg/L
4.427 J
A45O0F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
HB06030146
Water 12.5mg/L nitrite Tank #2
N02
9.9
mg/L
1.6425
A4500B
03/08/2009
03/08/2009 JB 6/3/2009
JB
HB08030147
Water 12.5mg/L nitrite Tank #3
JSI03
2,8
mg/L
4.427 J
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
HB08030147
Water 12.5mg/L nitrite Tank #3
N02
10.0
mg/L
1.6425
A4500B
03/08/2009
03/08/2009 JB 6/3/2009
JB
HB08030148
Water 12.5mg/L nitrite Tank #4
N03
3,1
mg/L
4.427 J
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
HB08G30148
Water 12,5mg/L nitrite Tank #4
N02
10,0
mg/L
1.6425
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030149R
Water 2 5m g/L nitrite Tank #1
N03
<0.5
mg/L
2.2135 U. HTv^
A4500F
14/10/2009
03/08/2009 Ben Cook
Ben Cook
HB08030149
Water 25mg/L nitrite Tank #1
N02
19.4
mg/L
3.285
A4500B
03/08/2009
02/08/2009 JB 8/3/2009
JB
HB08030150R
Water 25mg/L nitrite Tank #2
N03
<0.5
mg/L
2.2135 U,HTX
A4500F
14/10/2009
03/08/2009 Ben Cook
Ben Cook
HB08030150
Water 25mg/L nitrite Tank #2
N02
20.6
mg/L
3.285
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030151R
Water 25mg/L nitrite Tank #3
N03
<0.5
mg/L
2.2135 U.HT^
A45O0F
14/10/2009
03/08/2009 Ben Cook
Ben Cook
HB08030151
Water 25mg/L nitrite Tank #3
N02
20.0
mg/L
3.285
A45008
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030152R
Water 25mg/L nitrite Tank #4
N03
<0,5
mg/L
2.2135 U, HT^
A4500F
14/10/2009
03/08/2009 Ben Cook
Ben Cook
HB08030152
Water 25mg/L nitrite Tank #4
N02
20,8
mg/L
3.285
A45009
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030153R
Water 50mg/L nitrite Tank #1
N03
<1.1
mg/L
4.427 U, HT
A4500F
t4/10/2009
03/08/2009 Ben Cook
Ben Cook
HB08030153
Water 50rng/L nitrite Tank #1
N02
42.4
mg/L
8.2125
A4500B
03/08/2009
03/08/2009 JB 6/3/2009
JB
HB08030154AR Water 50mg/L nitrite Tank #2
N03
2.2
mg/L
4.427 J. HT
A4500F
14/10/2009
03/08/2009 Ben Cook
Ben Cook
HB08030154A
Water SOrng/L nitrite Tank #2
N02
42.2
mg/L
8.2125
A45006
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030154BR Water 50mg/L nitrite Tank #2
N03
2.7
mg/L
4.427 J, HT y
A4500F
14/10/2009
03/08/2009 Ben Cook
9en Cook
HB08030154B
Water 50mg/L nitrite Tank #2
N02
43,9
mg/L
8.2125
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB0S030155
Water 50mg/L nitrite Tank #3
N03
<11
mg/L
44.27 U
A45C0F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
H808030155
Water 50 m g/L nitrite Tank #3
N02
56.5
mg/L
8.2125
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030156R
Water 50m g/L nitrite Tank #4
N03
<1.1
mg/L
4.427 U, HTV/
A4500F
14/10/2009
03/08/2009 Ben Cook
Ben Cook
HB08030156
Water 50mg/L nitrite Tank #4
N02
47.1
mg/L
8.2125
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030157
Water 1OOmg/L nitrite Tank #1
N03
<11
mg/L
44.27 U
A4500F
03/08/2009
03/08/2009 Ben Cook 8/3/2009
Ben Cook
HB08030157A
Water 1OOmg/L nitrite Tank #1
N02
167
mg/L
16.425
A4500B
03/08/2009
03/08/2009 JB 3/3/2009
JB
HBOB030157B
Water 1 QOmg/L nitrite Tank #1
N02
170
mg/L
18.425 .
11.0675 J,HT^
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB0803015BR
Wafer 10Omg/L nitrite Tarsk #2
N03
8
mg/L
A4500F
14/10/2009
03/08/2009 Ben Cook
Ben Cook
HB08030158
Water 100m g/L nitrite Tank #2
N02
92
mg/L
16.425
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030159R
Water 100 m g/L nitrite Tank #3
N03
35
mg/L
11.0675 HT-^*
A4500F
14/10/2009
03/08/2009 Ben Cook
Ben Cook
HB08030159
Water 100m g/L nitrite Tank #3
N02
89
mg/L
16.425
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030160R
Water 100 m g/L nitrite Tank #4
N03
<3
mg/L
11.0675 U.HTV
A4500F
14/10/2009
03/08/2009 Ben Cook
Ben Cook
HB08030160
Water 100 m g/L nitrite Tank #4
N02
104
mg/L
16.425
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030161R
Stock Solution 3.2g/L NaN02-2.1g/l N03
<30
mg/L
110.675 U, HT
A4500F
14/10/2009
03/08/2009 Sen Cook
Ben Cook
HB09030161
Stock Solution 3.2g/L NaN02-2.1g/l N02
1880
mg/L
328.5
A4500B
03/08/2009
03/08/2009 JB 8/3/2009
JB
HB08030162R
Super Stock 32g/L NaN02-2tg/L N N03
<500
mg/L
2213.5 U.HTV-
A4500F
14/10/2009
03/06/2009 Ben Cook
Ben Cook
HB08030162
Super Stock 32g/L NaN02-21g/L N N02
23400
mg/L
3285
A45008
03/08/2009
03/08/2009 JB 8/3/2009
JB
HT: Holding time exceeded. Samlpes were re-run because original values were suspect
Page 1
-------
Nitrite Day -1
LabSamptelD
SampleDescriptton
ParamlO
Results Units
RL LabQualifier
H808040163
Water Control Tank #1
N02
0,0145
mg/L
0.016425 J
HB08Q40163
Water Control Tank #1
N03
1.089
mg/L
0.08854
HB08040164A
Water Control Tank #2
N02
0.01O2
mg/L
0.015425 J
HB08O4O1S4A
Water Control Tank #2
N03
1.256
mg/L
0.08854
HB080401B4B
Water Control Tank #2
N02
0.0115
mg/L
0.016425 J
HB08040164B
Water Control Tank #2
N03
1.067
mg/L
0.08854
HB08040165
Water Control Tank #3
N02
0.0181
mg/L
0.016425
HB08040165
Water Control Tank #3
N03
1.048
mg/L
0,08854
HB08040166
Water Control Tank #4
N02
0.1133
mg/L
0.016425
HB080401B6
Water Control Tank #4
N03
0.860
mg/L
0.08854
HB08040167
Water 6,25mg/L nitrite Tank #1
N03
0.85
mg/L
0,4427
HB08040167
Water 6.25mg/L nitrite Tank #1
N02
4.89
mg/L
0,82125
HB08040168
Water 6.25mg/L nitrite Tank #2
N02
5.00
mg/L
0.82125
HB080401S8
Waiter 6.25mg/L nitrite Tank #2
NQ3
0.55
mg/L
0.4427
HB08040169
Water 6.25mg/L nitrite Tank #3
N02
5.00
mg/L
0.82125
HBD8040169
Water 6.2Smg/L nitrite Tank #3
N03
0.27
mg/L
0.4427 J
HB08040170
Water B.2Smg/L nitrite Tank #4
N02
4.78
mg/L
0.82125
HB08040170
Water 6.25mg/L nitrite Tank #4
N03
1.11
mg/L
0.4427
HB08040171
Water 12.5mg/L nitrite Tank f 1
N02
10.2
mg/L
1.6425
HB08040171
Water 12.5mg/L nitrite Tank #1
N03
<.21
mg/L
0.8854 U
HB08040172
Water 12.5mg/L nitrite Tank #2
N02
10.4
mg/L
1.6425
HB08040172
Water 12.5mg/l nitrite Tank #2
N03
<.21
mg/L
0.8854 U
HBO8040173
Water 12.5mg/L nitrite Tank #3
N02
9.6
mg/L
1.6425
HB08040173
Water 12.5mg/L nitrite Tank #3
N03
0.51
mg/L
0.8854 J
HB08040174A
Water 12,5mg/L nitrite Tank #4
N02
10.3
mg/L
1,6425
HB08040174A
Water 12.5mg/l nitrite Tank #4
N03
<0.21
mg/L
0.8854 U
HB08040174B
Water 12,5mg/L nitrite Tank #4
N02
10,6
mg/L
1.6425
HB080401748
Water 12.5mg/L nitrite Tank #4
N03
<0,21
mg/L
0.8854 U
HB08040175
Water 25mg/L nitrite Tank #1
N02
21.7
mg/L
3.2B5
HBO8040175
Water 25mg/l nrtrite Tank #1
N03
0.9
mg/L
2.2135 J
HBO8040176
Water 25mg/L nitrite Tank #2
N02
21,7
mg/L
3.2B5
HB08040176
Water 25m g/L nitrite Tank #2
N03
<0.5
mg/L
2,2135 U
HB08040177
Water 25mg/L nitrite Tank #3
N02
23.4
mg/L
3.285
HB08040177
Water 25mg/L nitrite Tank #3
N03
<0.5
mg/L
2.2135 U
HBO804O178
Water 25mg/L nitrite Tank #4
N02
21.8
mg/L
3.285
HB08040178
Water 25mg/L nitrite Tank #4
NO 3
<0.5
mg/L
2.2135 U
HBO8040179
Water 50 mg/L nitrite Tank #1
N02
48.5
mg/L
8.2125
HB08040179
Water SOmg/L nitrite Tank #1
N03
<1.1
mg/L
4,427 U
HB08040180
Water 50mg/L nitrite Tank #2
N02
46.6
mg/L
8.2125
HB08040180
Water SOmg/L nitrite Tank #2
N03
<1.1
mg/L
4.427 U
HB080401B1
Water 50mg/L nitrite Tank #3
N02
49.2
mg/L
8.2125
HBQB040181
Water 50mg/L nitrite Tank #3
N03
<1,1
mg/L
4.427 U
HB08040182
Water 50rng/L nitrite Tank #4
N02
50,1
mg/L
8.2125
HB08040182
Water 50mg/L nitrite Tank #4
N03
<1.1
mg/L
4.427 U
HB0S040183
Water 100mg/L nitrite Tank #1
N02
106
mg/L
16.425
H808Q4Q1S3
Water 100mg/L nitrite Tank #1
N03
4.4
mg/L
11.0675 J
HB08040184A
Water 10Omg/L nitrite Tank #2
N02
97
mg/L
16.425
HB08040184AR Water 100mg/L nitrite Tank #2
N03
3
mg/L
11.0675 J, HT'
HB08040184B
Water 1 COmg/L nitrite Tank M2
N02
98
mg/L
16.425
HB0804D184BR Water 100mg/L nitrite Tank #2
N03
3
mg/L
11.0675 J, HT
MB08040185
Water lOOmg/L nitrite Tank #3
N02
100
mg/L
16,425
/
HBQ8040185R
Water 100mg/L nitrite Tank #3
N03
<3
mg/L
11,0675 U,
HTv
HB08040186
Water 1D0mg/L nitrite Tank #4
N02
98
mg/L
16.425
HB080401B6R
Water 10Omg/L nitrite Tank #4
N03
<3
mg/L
11.0675 U, HT
HT: Holding lime exceeded. Samlpes were re-run because original values were suspect.
32 Day Analytical Results 8.4.09
AnalysisMethod
A4500B
A4500F
A4500B
A4SGQF
A4500B
A4S00F
A4500B
A4500F
A4S00B
A4500F
A4SOOF
A4500B
A4500B
A4500F
A4500B
A4SOOF
A4500B
A4500F
A4S00B
A4S00F
A4500B -
A4S0DF
A4500B
A4500F
A4S00B
A4500F
A4SOOB
A4500F
A4500B
A4500F
A450DB
A4500F
A4500B
A4500F
A4500B
A4500F
A450QB
A4SOOF
A4500B
A4S00F
A4500B
A4500F
A450DB
A4500F
A4500B
A4500F
A4500B
A4500F
A4500B
A4500F
A4S00B
A4500F
A45008
A4500F
ArialysisDate
05/08/2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
07/06/2009
05/08/2009
07/08
05/08
07/08
07/08
05/08
2009
2000
2009
2009
2009
05/08/2009
07/08
05/08
07/03
05/08/2009
07/08
05/08
2009
2009
2009
2009
2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
D7/08/2009
05/08/2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
07/08/2009
05/08/2009
14/10/2009
05/08/2009
14/10/2009
05/08/2009
14/10/2009
05/08/2009
14/10/2009
SampleDate
04/06/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2008
04/08/2009
04/08/2009
04/08/2009
04/08/2009
04/08/2009
Analyst
Jt
Eton Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Ben Cook
Jl
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
J!
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Sen Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Calibration Date
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/7/2009
8/5/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
6/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/7/2009
8/5/2009
8/5/2009
B/5/2009
8/5/2009
Calibration Person
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Ben Cook
Jl
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
Jl
Ben Cook
J!
Ben Cook
Jl
Ben Cook
Page 1
-------
Nitrite DayO FHM 32 Day Analytical Results 8.7,09
LabSampletD
SampteOescription
ParamlD
Results
Units
RL LabQualifier
AnalysisMethod
AnalysisDate
SampleDate Analyst Calibration Date
Calibration Person
HB0B070212
Water control Tank #1
N02
0.0151
mg/L
0.016425 J
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08070212A
Water control Tank #1
N03
1.028
mg/L
0,08854
A4S00F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HBQ8070212B
Water control Tank #1
N03
1.085
mg/L
0.08B54
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070213
Water control Tank #2
N03
1.082
mg/L
0.08854
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070213
Water control Tank #2
N02
0.0161
mg/L
0.016425 J
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
MB08070214
Water control Tank #3
N03
1.064
mg/L
0.08854
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070214
Water control Tank #3
N02
0,0141
mg/l
0.01642S J
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB0BO7O21S
Water control Tank #4
N03
1,035
mg/L
0.08854
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070215A
Water control Tank #4
N02
0.0200
mg/L
0.016425
A4500B
07/08/2009
07/08/2009 JI 8/7/2009
Jl
HB08070215B
Water control Tank #4
N02
0.0200
mg/L
0.016425
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08070216
Water 6.25mg/l nitrite Tank #1
N03
0.77
mg/L
0.4427
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070216
Water 6.25mg/l nitrite Tank #1
N02
5.7
mg/L
1.6425
A4500B
07/08/2009
07/06/2009 Jl 8/7/2009
Jl
HB0807Q217
Water 6,25mg/L nitrite Tank #2
N03
0.93
mg/L
0.4427
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070217
Water 6.25mg/L nitrite Tank #2
N02
5.8
mg/L
1.6425
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HBQ8070218
Water 6.25mg/L nitrite Tank #3
N03
<0.11
mg/L
0.4427 U
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HBO8O70218
Water 6.25mg/L nitrite Tank #3
N02
7.0
mg/L
1.6425
A4500S
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB0B070219
Water 6,25m g/L nitrite Tank #4
N03
<0.11
nug/L
0.4427 U
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HBO807Q219
Water 6.25mg/L nitrite Tank #4
N02
6.5
mg/L
1.6425
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
J[
HB0807Q220
Water 12.5mg/L nitrite Tank #1
N03
0.37
mg/L
0.8854 J
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HBO8070220
Water 12.5mg/L nitrite Tank #1
N02
11.3
mg/L
3.285
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08070221
Water 12.5mg/L nitrite Tank #2
N03
1.75
mg/L
0.8854
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070221
Water 12.Smg/L nitrite Tank #2
N02
10.0
mg/L
3.285
A4S00B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08070222
Water 12,5mg/L nitrite Tank #3
N03
1.00
mg/L
0.8854
A45O0F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HBQ8G70222
Water 12,5mg/L nitrite Tank #3
N02
11,0
mg/L
3.285
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08070223
Water 12.5mg/L nitrite Tank #4
N03
<0.21
mg/L
0.8854 U
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070223
Water 12.5mg/L nitrite Tank #4
N02
12.4
mg/L
3.285
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08070224A
Water 25mg/L nitrite Tank #1
N03
1.8
mg/L
2.2135 J
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070224A
Water 25mg/L nitrite Tank #1
N02
23.6
mg/L
4.10625
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08070224B
Water 25mg/L nitrite Tank #1
NO 3
2.8
mg/L
2.2135
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Sen Cook
HB08070224B
Water 25mg/L nitrite Tank #1
N02
23.1
mg/L
4.10625
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08Q7022S
Water 25mg/L nitrite Tank #2
N03
1.8
mg/L
2.2135 J
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070225
Water 25mg/L nitrite Tank #2
N02
24.6
mg/L
4.10625
A4500B
07/08/2009
07/08/2009 J! 8/7/2009
Jl
HB08070226
Water 25mg/L nitrite Tank #3
NQ3
4.3
mg/L
2.2135
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HBQ8070226
Water 2Smg/L nitrite Tank #3
N02
20.3
mg/L
4.10625
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
J!
HBG8070227
Water 25mg/L nitrite Tank #4
N03
<0.5
mg/L
2.2135 U
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070227
Water 25mg/L nitrite Tank #4
N02
24.2
mg/L
4.10625
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08070228
Water 50rag/L nitrite Tank #1
N03
4.2
mg/L
4.427 J
A4500F
07/08/2009
07/OB/2009 Ben Cook 8/7/2009
Ben Cook
HB08070228
Water 50mg/L nitrite Tank #1
N02
53.7
mg/L
8,2125
4.427 J, HT^
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HBO807O229R
Water 50mg/L nitrite Tank #2
N03
1.8
mg/L
A4500F
14/10/2009
07/08/2009 Ben Cook
Ben Cook
HB08070229
Water 50mg/L nitrite Tank #2
N02
49.5
mg/L
8.2125
A450QB
07/08/2009
07/08/2009 JI 8/7/2009
Jl
HB08070230
Water 50mg/L nitrite Tank #3
N03
6.0
mg/L
4.427
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070230
Water SOmg/L nitrite Tank #3
N02
52.2
mg/L
8.2125
A4500B
07/06/2009
07/08/2009 Jl 8/7/2009
J!
HB08070231
Water SOmg/l. nitrite Tank #4
N03
2,1
mg/L
4.427 J
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070231
Water 50mg/L nitrite Tank #4
N02
54.5
mg/L
8.2125
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08070232R
Water lOOmg/L nitrite Tank #1
N03
<3
mg/L
11.0675 U, HTV
A4500F
14/10/2009
07/08/2009 Ben Cook
Ben Cook
HBOB070232
Water "lOOmg/L nitrite Tank #1
N02
96
mg/L
16.425
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08070233R
Water 1OOmg/L nitrite T ank #2
N03
<3
mg/L
11.0675 U, HTj^-
A4500F
14/10/2009
07/08/2009 Ben Cook
Ben Cook
HB08070233
Water 100 nig A. nitrite Tank #2
N02
82
mg/L
16.425
A45O0B
07/08/2009
07/08/2009 JI 8/7/2009
Jl
HB08070234
Water 10Omg/L nitrite Tank #3
N03
26.6
mg/L
13,281
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070234A
Water lOOmg/L nitrite Tank #3
N02
90
mg/L
16.425
A4500B
07/08/2009
07/08/2009 Jl 8/7/2009
Jl
HB08070234B
Water 100mg/L nitrite Tank #3
N02
90
mg/L
16.425
A4500B
07/08/2009
07/08/2009 Jl 8/7/2003
Jl
HB08070235
Water 100mg/L nitrite Tank #4
NOS
15.5
mg/L
13.281
A4500F
07/08/2009
07/08/2009 Ben Cook 8/7/2009
Ben Cook
HB08070235
Water 100mg/L nitrite Tank #4
N02
94
mg/L
16.425
A4500B
07/08/2009
07/08/2009 J! 8/7/2009
Jl
HT: Holding time exceeded, Samlpes were re-run because original values were suspect
Page 1
-------
Nitrite Day 3 FHM 32 Day Analytical Results 8.10.09
LabSamplelD
SampleDescription
ParamID
Results
Units
RL LabQualffler
AnalysisMethod
Analysts Date
SampleOate
Analyst
Calibration Date
Calibration Person
H808100246
overlying water tank #1
control Nitrit N03
0.853
mg/L
0.08854
A4500F
13/08/2009
10/08/2000
Ben Cook
8/13/2009
Ben Cook
HB08100246
overlying water tank #1
control Nitrit N02
0.0145
mg/L
0.016425 J
A4500B
12/08/2009
10/08/2009
Jl
8/12/2009
Jl
HB08100247
overlying water tank #1
6,25mg/L Ni N03
<0.21
mg/L
0.8854 U
A4500F
13/08/2009
10/08/2009
Ben Cook
8/13/2009
Ben Cook
HBD8100247
overlying water tank #1
6.25tng/L Ni N02
5.22
rng/U
0.82125
A4500B
12/08/2009
10/08/2009
Jl
8/12/2009
Jl
HB08100248
overlying water tank #1
25mg/L Mitri N03
<1.1
mg/L
4.427 U
A4500F
13/08/2009
10/08/2009
Ben Cook 8/13/2009
Ben Cook
HB08100248
overlying water tank #1
25mg/L Nitri N02
24,0
mg/L
3.285
A4500B
12/08/2009
10/08/2009
Jl
8/12/2009
Jl
HB08100249R
overlying water tank #1
100rng/L Nit N03
56
mg/L
11.0675 HT y
A4500F
14/10/2009
10/08/2009
Ben Cook
Ben Cook
HB08100249
overlying water tank #1
1OOmg/L Nit N02
51
mg/L
16.426
A45008
12/08/2009
10/08/2009
Jl
8/12/2009
Jl
HT: Holding time exceeded. Samlpes were re-run because original values were suspect.
Page 1
-------
Nitrite DayS FHM 32 Day Analytical Results 8,12.09
I LabSamplelD
N HB08120266
SampteDescription ParamID
overlying water tank #2 control Nitrit N03
Results
0,969
Units
mg/L
RL LabQualifier
0.08854
AnalysisMethod
A45Q0F
AnalysisDate
13/08/2009
SampleOate Analyst Calibration Date
12/08/2009 Ben Cook 8/13/2009
Calibration Person
Ben Cook
HBOB120266
overlying water tank #2 control Nitrit- N02
0.0205
ma/L
0.016425
A4500B
12/08/2009
12/08/2009 Jl 8/12/2009
Jt
j HB08120267
*' HB08120267
overlying water tank #1 12.5mg/l Ni N03
<0,42
mg/L
1.7708 U
A4500F
13/08/2009
12/08/2009 Ben Cook 8/13/2009
Ben Cook
oveftyinq water tank #1 12.5mq/L Ni N02
13.8
mn/L
1.6425
A4500B
12/08/2009
12/08/2009 Jl 8/12/2009
Jt
/ HB08120268
overlying water tank #1 50mg/L Nitri N03
<1,3
mg/L
5.53375 U
A4500F
13/08/2009
12/08/2009 Ben Cook 8/13/2009
Ben Cook
HB08120268A
overlying water tank #1 50mg/L Nitri N02
57.6
mg/L
8.2125
A4500B
12/08/2009
12/08/2009 Jl 8/12/2009
Jl
HBQ8120268B
overlying water tank #1 50mg/L Nitri N02
57.1
mg/L
8.2125
A4500B
12/08/2009
12/08/2009 Jl 8/12/2009
Jl
Page 1
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Nitrite Day 7 FHM 32 Day Analytical Results 8.14.09
I LabSamplelD
SampleDescription ParamiD
Results
Units
RL LabGualifier
AnalysisMethod
AnalysisDate
SampleDate Analyst Calibration Date
Calibration Person
J HBQ8140279
overtying water tank #3 control Nitrit N02
<0.0036 mg/L
0.016425 U
A4500B
14/08/2009
14/08/2009 Jl 8/14/2009
Jl
HB08140279A
overlying water tank #3 control Nitrit N03
1,097
mg/L
0.08854
A4500F
14/08/2009
14/08/2009 Ben Cook 8/14/2009
Ben Cook
HB08140279B
overlying water tank #3 control Nitrit- N03
1.116
rng/L
0.08854
A4500F
14/08/2009
14/08/2009 Ben Cook 8/14/2009
Ben Cook
/ HB0814Q280
overlying water tank #2 6.25mg/L Ni N03
1-32
mg/L
0.68405
A4500F
14/08/2009
14/08/2009 Ben Cook 8/14/2009
Ben Cook
^ HB08140280
overlying water tank #2 6.25mg/L Ni NQ2
5.7
rng/L
1.6425
A4500B
14/08/2009
14/08/2009 Jl 8/14/2009
Jl
/ HB08140281
overtying water tank #2 25mg/L Nitrt NU2
19
rng/L
16.425
A4500B
14/08/2003
14/08/2009 Jl 8/14/2009
Jl
^ HB08140281
overlying water tank #2 25mg/L N'rtri N03
rng/L
2.2135
A4500F
14/08/2009
14/08/2009 Ben Cook 8/14/2009
Ben Cook
J HBOB140282
overlying water tank #2 100rng/L Nit N02
113
mg/L
16.425
A4500B
14/08/2009
14/08/2009 Jl 8/14/2009
Jl
, HBOB140282
overlying water tank #2 100mg/t Nit N03
mg/L
8.854
A4S00F
14/08/2009
14/08/2009 Ben Cook 8/14/2009
Ben Cook
1 HB0B14O283
Stock solution 2.1g/L N02 3.0g/L Ni N02
2450
mg/L
1642.5
A450QB
14/08/2009
14/08/2009 Jl 8/14/2009
Jl
~J HB08140283
Stock solution 2.1g/L N02 3.0g/L N« N03
<50
rng/L
221.35 U
A4500F
14/08/2009
14/08/2009 Ben Cook 8/14/2009
Ben Cook
Page 1
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Nitrite Day 10 FHM 32 Day Analytical Results 8.17.09
LabSamplelD SampleDescription ParamID Results Units RL LabQualifier AnalysisMethod AnalysisDate SampleDate Analyst Calibration Date Calibration Person
HB08170293 overlying water tank W control Nitrit N03 1.068 mg/L 0.08854 A4500F 17/08/2009 17/08/2009 Ben Cook 8/17/2009 Ben Cook
HBOB 170293 oveflying water tank #4 control Nltrit- N02 0.0237 mg/L 0.016425 A4500B 19/08/2009 17/08/2009 JI 8/18/2009 Ji
HB08170294A overlying water tank #212.5 Nitrite-! N03 <0.5 mg/L 2.2135 U A4500F 17/08/2009 17/08/2009 Ben Cook 8/17/2009 Ben Cook
HB08170294A overlying water tank #2 12.5 Nitflte-I N02 14.1 mg/L 1.6425 A4500B 19/08/2000 17/08/2009 JI 8/19/2009 JI
H8Q8170294B overlying water tank #212.5 Nltrite-I N03 <0.5 mg/L 2,2135 U A4500F 17/08/2009 17/08/2009 Ben Cook 8/17/2009 Ben Cook
HB08170294B oveflying water tank #212 5 Nitnte-I N02 14.7 mg/L 1.6425 y A4500B 19/08/2009 17/08/2009 Jl 8/19/2009 JI
HB0817029SR oveflying water tank #2 50 Nitnte-D, N03 <1.1 mg/L 4.427 U, HT A4500F 14/10/2009 17/08/2009 Ben Cook Ben Cook
HB08170295 overlying water tank *2 50 Nitrite-Di N02 60.8 mg/L 8,2125 A4500B 19/08/2009 17/08/2009 JI 8/19/2009 JI
HT: Holding time exceeded. Samlpes were re-run because original values were suspect.
Page 1
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Nitrite Day 12 FHM 32 Day Analytical Results B.19.09
| LabSamplelD SampleDescription ParamID Results Units RL LabQualifier Analysis Method AnalysisOate SampleDate Analyst Calibration Date Calibration Person
>4 H0OB190306 overlying water tank#! control-Day 12 N03 1.104 mg/l 008854 A4SO0F 21/08/2009 19/08/2009 Ben Cook 8/21/2009 Ben Cook
HBOB190306 overtvino water tank «1 control-Pay 12 NQ2 0.0088 mg/L 0.016425 J A4500B 19/08/2009 19/08/2009 Jl 8/19/2009 Jj
/ HBOB19030? overlying water tank #3 6,25mg/L-Day 12 NG3 0.44 mg/L 0.4427 J A4500F 21/08/2009 19/08/2009 Ben Cook 8/21/2009 Ben Cook
^ , HB0819P3P7 overlying water tank #3 6.2Smo/L-Pay 12 NQ2 7.93 mult 0.82125 A45Q06 19/08/2009 19/08/2009 Jl 8/19/2009 Jj
I HB08190308 overlying water tank #3 25mg/L-Day 12 N03 <15 irigTL 2.2135 U A4E00F 21/08/2009 19/08/2009 Ben Cook 8/21/2009 Ben Cook "
i HB08190308 overtvino water tank <3 25m
-------
Nitrite Day 14 FHM 32 Day Analytical Results 8.21.09
LabSamplelD
SampleDescription
ParamID
Results Units
RL LabQualifier
AnalysisMethod AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Person
HB08210318
overlying water tank #2 control Nitrite-Day 14
N02
0.0140
mg/L
0,016425 J
A45006
21/08/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
H8O8210318A
overlying water tank #2 control Nitrite-Day 14
N03
1.097
mg/l
0.08854
A4500F
21/08/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
HB08210318B
overlying water tank #2 control Nitrite-Day 14
N03
1.088
mg/L
0.03854
A4500F
21/08/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
overlying water tank #3 12.5mg/L Nitrite-Day
HB08210319
14
N02
13.2
mg/L
1.6425
A450QB
21/08/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
overlying water tank #3 I2.5mg/L Nitrite-Day
HB08210319
14
N03
2.09
mg/L
0.8854
A4500F
21/08/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
HB08210320
overlying water tank #3 SOmg/L Nitrite-Day 14
N02
49.2
mg/L
8.2125 /
A4500B
21/08/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
HBQ821032OR
overtying water tank #3 50mg/L Nitrite-Day 14
N03
<1.1
mg/L
4.427 U, HT ^
A4S00F
14/10/2009
21/08/2009 Ben Cook
Ben Cook
HT: Holding time exceeded. Samlpes were re-run because original values were suspect.
Page 1
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Nitrite Day 17 FHM 32 Day Analytical Results 8.24,09
J
J
LabSamplelD SampleDescription ParamID Results Units
HB08240349 overlying water tank #3 control Nitrite-Day 17 ND3 1,052 mg/L
HR08240349 overlying water tank #3 control Nitrite-Day 17 N02 <0.0036 mfl/L
HB0824Q350
HB0B24035Q
overlying
overlying
RL LabGualifier AnalysiaMethod AnalysisDate SampleDate Analyst Calibration Date
0.08854 A4500F 24/08.2009 24/08/2009 Ben Cook 8/24/2009
0.016425 U A4S0OB 24/08/2009 24/08/2009 Jl 8/24/2009
water tank #4 B.25mg/L Nitrite-Day 1'N03 0.72 mg/L 0.4427
water tank ttA 6 25mg/L Nitrite-Day 1" NQ2 8.1 mg/L 1,6425
A4500F
A4500B
24/06/2009
24/08/2009
24/06/2006 Ben Cook B/24/20U8
24/08/2009 Jl 8/24/2009
Calibration Person
Ben Cook
Jl
'BSfTC'MK
_JJ
7
HB08240351
HBQ8240351
overlying
overivino
water tank
water tank
#4 25mg/L Nitrite-Day 17 N03
#4 25mo/L Nitrite-Day 17 NQ2
2.3
26.4
mg/L
mo/L
2.2135
4.10625
A4500F
A4500B
24/08/2008
24/08/2009
24/08/2009 Ben Cook 8/24/2009
24/08/2009 Jl 6/24/20Q9
Ben Cook
Jl _
HB06240352
HB08240352
overly
overly
water tank
water tank
#4 100mg/L Nitrite-Day 17 N03
#4 lOOrng/L Nitrtte-Day 17 N02
<1.1
107
mg/L
mg/L
4.427 U
16.425
A4500F
A4500B
24/08/2009
24/08/2009
24/08/2009 Ben Cook 8/24/2009
24/08/2009 Jl 8/24/2009
Ben Cook
Jl
Page 1
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Nitrite Day 19 FHM 32 Day Analytical Results 8.26.09
J
J
LabSamplelD SampteDescription ParamID Results Units RL
overlying water tank #4 control
HB0826O381 Nitrite-Day 19 N03 1.343 mg/L 0.08854
overlying water tank #4 control
HB082603B1A Nitrite-Day 19 NQ2 0.0247 mg/L 0.016425
LabQualifier AnalysisMethod AnalysisDate SampteDate Analyst Calibration Date Calibration Person
A4500F
A4500B
28/08/2009
28/03/2009
08/2009 Ben Cook 8/28/2009
08/2009 JI 8/28/2009
Ben Cook
Ji
overlying water tank #4 control
HB082603B1B Nitrite-Day 19 N02
overlying water tank #4 12.femg/L
HB08260382 Nitrite-Day 19 ND3
overlying water tank #4 12.5mg/L
HB08260382 Nitrite-Day 19 ND2
0.0200 mg/L 0.01642S
A4SOOB
28/08/2009 26
08/2009 JI
8/28/2009
JI
J
1
<.21 mg/L 0.8854 U A4500F 28/08/2009 26
13.1 mg/L 3.285 A4500B 28/08/2009 26
08/2009 Ben Cook 8/28/2009
08/2009 JI 8/28/2009
Ben Cook
JI
HB08260383
HB08260383
overlying water tank #4 50mg/L
Nilrite-Day 19
overlying water tank #4 50mg/l
Nitrite-Day 19
N03
N02
<1.1 mg/L 4.427 U
48.8 mg/L 8.2125
A4500F
A4500B
28/08/2009 26
28/06/2009 26
08/2009 Ben Cook 8/28/2009
08/2009 JI 8/28/2009
Ben Cook
JI
Page 1
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Nitrite Day 20 FHM 32 Day Analytical Results 8.27.09
LabSampIelD
SampleDescription ParamID
Results
Units
RL LabQuplifiet
0.4427 HT
AnalysisMethod
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Person
HB08270390R
Water 6.25mg/L nitrite Tank #1 N03
1.2
mg/L
A4500F
14/10/2009
27/08/2009 Ban Cook
Ben Cook
HB08270390A
Water 6.25mg/L nitrite Tank #1 N02
2.3
mg/L
1.6425
A4S00B
28/08/2009
27/08/2009 Jl
8/28/2009
Jl
HB0827O39QB
Water 6.25mg/L nitrite Tank #1 N02
2.4
mg/L
1.8425
A4500B
28/08/2009
27/08/2009 Jl
8/28/2009
Jl
HB08270391R
Overiying water Tank #1 12.5mg/L r N03
Overlying water Tank #1 12.5mg/L
1.0
mg/L
0.4427 HTv/
A4S00F
14/10/2009
27/08/2009 Ben Cook
Ben Cook
HB0B270391
nitrite N02
4.6
mg/L
3.285 ,
0,8854 HT
A4500B
28/08/2009
27/08/2009 Jl
8/28/2009
Jl
HB0827Q392R
Overlying water Tank #1 25mg/L niti N03
Overlying water Tank #1 25mg/L
2.4
mg/L
A4500F
14/10/2009
27/08/2009 Ben Cook
Ben Cook
HB08270392
nitrite N02
9.3
mg/L
4.10625 ^
2.2135 HT *
A4500B
28/08/2009
27/08/2009 Jl
8/28/2009
Jl
HB08270393R
Overlying water Tank #1 50mg/L niti N03
Overlying water Tank #1 50mg/L
6.6
mg/L
A4500F
14/10/2009
27/08/2009 Ben Cook
Ben Cook
HB08270393
nitrite N02
Overlying water Tank #1 100mg/L
15.2
rng/L
8.2125
A450QB
28/08/2009
27/08/2009 Jl
8/28/2009
Jl
HB08270394
nitrite N03
<2.1
mg/L
8.854 U
A4500F
28/08/2009
27/08/2009 Ben Cook 8/2B/2009
Ben Cook
Overling water Tank #1 1QQmg/L
HB08270394
nitrite N02
35
mg/L
16.425
A4500B
28/08/2009
27/08/2089 Jl
8/28/2009
Jl
HT: Holding time exceeded. Samlpes were re-run because original values were suspect.
Page 1
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Nitrite Day 21 FHM 32 Day Analytical Results 8,28.09
J
±
LabSamplelD
HB08280395
HB0828039S
SampleDescription PararnlD Results Units
overlying water tank #1 control
Nitrite-Day 21 NQ3 1,327 mg/L
overlying water tank #i control
Nitrite-Day 21 N02_ 0.0043 mg/L
RL LabQualifier AnalysisMethod ArialysisDate SampleDate Analyst Calibration Date Calibration Person
0.08654 A4500F 28/08/2009 28/08/2009 Ben Cook 8/28/2009 Ben Cook
0.016425 J A4500B 28/08/2009 28/08/2008 J[ 8/28/2009 Jl__
overlying water tank #1 6.25mg/L
H608280396 Nitrite-Day 21 N03 1.172 mg/L 0.4427
overlying water tank #1 6.25mg/L
HB08280396A Nitrite-Day 21 N02 S.O mg/L 1.6425
overlying water tank #1 6.25mg/L
HB08280396B Nitrite-Day 21 N02 5.3 mg/L 1,6425
A4500F
A4500B
A4500B
28/08/2009 28/08/2009 Ben Cook 8/28/2009
28/08/2009 28/08/2009 Jl 8/28/2009
28/08/2009 28/08/2009 Jl 8/28/2009
Ben Cook
Jl
Jl
overlying water tank #1 25mg/L
26/08/21309 Btilt CuuK 8/28/2009 Ben Ccok1
28/08/2008 Jl 8/28/2009 Jl
J
HBQ8280397
HB08280397
Nitriie-Day 21 ~N03
-------
Nitrite Day 24 FHM 32 Day Analytical Results 8.31.03
LabSamplelD
Sample Description
overlying wat tank 2 contii Nitrite -
ParamiD
Results
Units
RL LabQualifier
AnalysisMethod
AnalysisDate
SampleDate Analyst Calibration Date
Calibration Person
HB08310401
Day 24
overlying wat tank 2 contrl Nitrite -
N02
0.0079
mg/L
0.016425 J
A4500B
02/09/2009
31/08/2009 J[ 9/2/2009
Jl
HB08310401A
Day 24
overlying wat tank 2 oontrl Nitrite -
N03
1.24?
mg/L
0,08854
A4500F
11/09/2009
31/08/2009 Ben Cook 9/11/2009
Ben Cook
HB08310401B
Day 24
overlying wat lank 1 12.5mg/i Nitrite
N03
1.147
mg/L
0.088S4
A4500F
11/09/2009
31/08/2009 Ben Cook 9/11/2009
Ben Cook
HBQ8310402
- Day 24
overlying wat tank 1 12.5mg/i Nitrite
N03
1.57
mg/L
0.8864
A4500F
11/09/2009
31/08/2009 Bert Cook 9/11/2009
Ben Cook
HB08310402A
-Day 24
overlying wat tank 1 12,5mg/t Nitrite
N02
9,3
mg/L
3.285
A4500B
02/09/2009
31/08/2009 Jl 6/2/2009
Jl
HB08310402B
- Day 24
N02
9.2
mg/L
3.285 ^
A4500B
02/09/2009
31/08/2009 Jl 9/2/2009
Jl
HB0831Q403R
overlying wat tank 1 SOmg/l Nitrite -
overlying wat tank 1 50mg/l Nitrite -
N03
2.2
mg/L
4.427 J, HT
A4500F
14/10/2009
31/08/2009 Ben Cook
Ben Cook
HB08310403
Day 24
N02
44.5
mg/L
8.2125
A4500B
02/09/2009
31/08/2009 Jl 9/2/2009
Jl
HT: Holding time exceeded, Samlpes were re-run because original values were suspect.
Page 1
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Nitrite Day 25 FHM 32 Day Analytical Results 9.1.09
LabSamplelD
SampleDescription
ParamID
Results
Units
RL LabQualifier
AnalysisMethocf
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Person
Water 6.25 mg/l nitrite Tank 2 day
HB09010404
25
N02
2.8
mg/L
1.6425
A4500B
02/09/2009
01/09/2009 Jl
9/2/2009
Jl
Water 6.25 mg/l nitrite Tank 2 day
HB09010404A
25
N03
1.41
mg/l
0.4427
A4500F
11/09/2009
01/09/2008 Ben Cook
9/11/2009
Ben Cook
Water 6.25 mg/l nitrite Tank 2 day
HB090104Q4B
25
N03
1.2a
mg/L
0.4427
A4500F
11/09/2009
01/09/2009 Ben Cook
9/11/2009
Ben Cook
Water 12.5 mg/l nitrite Tank 2 day
HB09010405
25
N03
<0.21
mg/l
0.8854 U
A4500F
11/09/2009
01/09/2009 Ben Cook
9/11/2009
Ben Cook
Water 12,5 mg/i nitrite Tank 2 day
HB08010405
25
N02
10.6
mg/L
3.285
A4500B
02/09/2009
01/09/2009 J!
9/2/2009
Jl
HB09010406
Water 25 mg/l nitrite Tank 2 day 25
N03
<0.5
mg/L
2.2135 U
A4500F
11/09/200S
01/08/2009 Ben Cook
9/11/2009
Ben Cook
HB09010406
Water 25 mg/l nitrite Tank 2 day 25
N02
22.2
mg/L
4.10625
A4500B
02/09/2009
01/09/2009 Jl
9/2/2009
Jl
HB0901040?
Water 50 mg/l nitrite Tank 2 day 25
N03
4.7
mg/L
4.427
A4500F
11/09/2009
01/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09010407
Water 50 mg/l nitrite Tank 2 day 25
N02
49.2
mg/L
8.2125
A450QB
02/09/2009
01/09/2009 Jl
9/2/2009
Jl
Water 100 mg/l nitrite Tank 2 day
HB09010408
25
N03
14.4
mg/L
11.0675
A4500F
11/09/2009
01/09/2009 Ben Cook
9/11/2009
Ben Cook
Water 100 mg/l nitrite Tank 2 day
HB09010408
25
N02
97
mg/L
16.425
A4500B
02/09/2009
01/09/2009 Jl
9/2/2009
Jl
Page 1
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Nitrite Day 28 FHM 32 Day Analytical Results 9.2.09
LabSamplelD
Sample Description
ParamlO
Results Units
RL LabQualifier
AnalysisMethod
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Person
overlying wat tank 3 contri Nitrite -
HB09020409
Day 26
overlying wat tank 3 contri Nitrite •
N02
<0.0036
rng/L
0.016425 U
A4500B
02/09/2009
02/09/2009 Jl
9/2/2009
Jl
HB09020409A
Day 26
overtying wat tank 3 contri Nitrite -
N03
1.252
mg/L
0.08854
A4500F
11/09/2009
02/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09020409B
Day 26
overlying wat tank 2 6.25mg/l Nitrite
N03
1.227
rng/L
0.08854
A4500F
11/09/2009
02/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09Q20410
- Day 26
overlying wat tank 2 6.25mg/l Nitrite
N03
0.33
rng/L
0.4427 J
A4500F
11/09/2009
02/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09020410
- Day 26
overlying wat tank 2 25rng/l Nitrite -
N02
6.4
mg/L
1.6425
A4500B
02/09/2009
02/09/2009 Jl
9/2/2009
Jl
HB09020411
Day 26
overlying wat tank 2 25mg/l Nitrite -
N03
0.7
mg/L
2.2135 J
A4500F
11/09/2009
02/09/2009 Ben Cook
8/11/2009
Ben Cook
HB09Q20411
Day 26
NQ2
25.8
mg/L
4.10625 /
A4500B
02/09/2009
02/09/2009 Jl
9/2/2009
Jl
HB09020412R
overlying wat tank 2 IQOmg/l Nitrite N03
<3
mg/L
11.0675 U, HT
A4500F
14/10/2009
02/09/2009 Ben Cook
Ben Cook
overlying wat tank 2 10Omg/l Nitrite-
HB0902Q412A
Day 26
overlying wat tank 210Qmg/l Nitrite ¦
N02
106
mg/L
16.425
A4500B
02/09/2009
02/09/2009 Jl
9/2/2009
Jl
HB09020412B
Day 28
Stock Sain 2.1 g/l N02 3g/l NaN02
N02
102
mg/L
16.425
A4500B
02/09/2008
02/09/2003 Jl
9/2/2009
Jl
HBD9020413
- Day 28
Stock Soln 2.1 g/l N02 3g/l NaN02
N03
<27
mg/L
110.675 U
A4500F
11/09/2009
02/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09020413
- Day 26
N02
2360
mg/L
328.5
A4500B
02/09/2009
02/09/2009 Jl
9/2/2009
Jl
HT: Holding time exceeded. Samlpes were re-wn because original values were suspect.
Page 1
-------
Nitrite Day 28 FHM 32 Day Analytical Results 9.4.09
J
LabSamplelD SampleDescription ParamID Results Units RL LabQualifier AnaiysisMethod AnalysisUate SampleDate Analyst Calibration Date Calibration Person
overlying wat tank 4 contri Nitrite -
4 Day 28 N02 0.0096 mg/t 0,016425 J A45008 04/08/2009
overlying wat tank 4 contri Nitrite -
4A Day 28 N03 1.137 mg/L 0.08854 A45G0F 11/09/2000
overlying wat tank 4 contri Nitrite -
43 Day 28 NQ3 1.129 mg/L 0.08854 A4500F 11/09/2009 04/09/2009 Ben Cook 9/11/2009 Ben Cook
HB090404
H8090404
HB090404
04/09/2009 JI 3/4/2009
04/09/2009 Ben Cook 9/11/2009
Jl
Ben Cook
>1
HB090404
HB090404
HB090404
overlying wat tank'2 T2.5mg/I Nitrite
5 -Day 28 N03 1.05 mg/L 0.8854
overlying wat tank 2 12.5mg/l Nitrite
5A -Day 28 N02 13.5 mg/L 3.285
overlying wat tank 2 12.5mg/l Nitrite
5B - Day 28 N02 14,1 mg/L 3.285
A4500F
A4500B
A450QB
11/09/2009 04/09/2009 Ben Cook 9/11/2009 Ben Cook
04/08/2009 04/09/2009 Jl 9/4/2009 Jl
04/09/2009 04/09/2009 Jl 9/4/2009 Jl
J
overlying wat tank 2 50mg/l Nitrite -
HB09040416 Day 28 N03 mg/L 8.854 J A4500F
overlying wat tank 2 50mg/l Nitrite -
HB09040416 Day 26 N02 55.6 mg/L 8.212S A4500B
11/09/2009 04/09/2009 Ben Cook 9/11/2009 Ben Cook
04/09/2009 04/09/2009 Jl 9/4/2009 Jl
Page 1
-------
Nitrite Day 32 FHM 32 Day Analytical Results 9.8.09
LsbSamplelD
SampleDescriptlon
ParamID
Results
Units
RL LabQualrfier
AnalysisMefhod
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Person
H6090B0417
Water control Tank #1
N03
1.144
mg/L
0.08854
A450OF
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09Q8041?
Water control Tank #1
N02
0.0049
mg/L
0.016425 J
A4500B
08/09/2009
08/09/2009 Jl
9/8/20O9
Jl
HB09080418
Water control Tank #2
N03
1.125
mg/L
0.08854
A450OF
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HBQ9080418
Water control Tank #2
N02
0.0125
mg/L
0.018425 J
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB09080419
Water control Tank #3
NQ3
1.037
mg/L
0.08854
A4500F
11/09/2009
O8/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080419
Water control Tank #3
N02
0.0082
mg/L
0.016425 J
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB0908042Q
Water control Tank #4
NQ3
1.126
mg/L
0.08854
A4500F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080420
Water control Tank #4
N02
0.0232
mg/L
0.016425
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB08G80421
Water 6.25mg/L nitrite #1
N03
0.61
mg/L
0.4427
A4500F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080421
Water 6.25mg/l nitrite #1
NQ2
6.7
mg/L
1.6425
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB09080422
Water 6 25mg/L nitrite #2
N03
1.73
mg/L
0.4427
A45O0F
11/00/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080422
Water 6,25mg/L nitrite #2
N02
6.0
mg/L
1,6425
A45008
08/09/2009
08/09/2009 Jl
9/8/2009
Ji
HB09080423
Water 6.26mg/L nitrite #3
N03
<0.11
mg/L
0.4427 U
A45O0F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080423
Water 6,25mg/L nitrite #3
N02
7.6
mg/L
1.6425
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB0908D424
Water 6.25mg/L nitrite #4
N03
0.13
mg/L
0.4427 J
A4500F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080424A
Water 6.25mg/L nitrite #4
N02
6.6
mg/L
1.6425
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB09080424B
Water 6.2Smg/L nitrite #4
N02
6.8
mg/L
1.6425
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB09080425
Water 12.5mg/L nitrite #1
N03
1.69
mg/L
0.8854
A4500F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080425
Water 12.5mg/L nitrite #1
N02
12.3
mg/L
3.285
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
H809080426
Water 12,5mg/L nitrite #2
N03
2.94
mg/L
0.8854
A4500F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080426
Water I2,5mg/L nitrite #2
N02
10.7
mg/L
3.285
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB09080427
Water I2.5mg/L nitrite #3
N03
0.93
mg/L
0.8854
A450QF
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080427
Water 12.Smg/L nitrite #3
N02
12.6
mg/L
3.285
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB09080428
Water 12,Smg/L nitrite #4
N03
<0.21
mg/L
0.8854 U
A4500F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080428
Water 12.5mg/L nitrite #4
N02
13.7
mg/L
3,285
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
JI
HB09080429
Water 25mg/L nitrite #1
N03
3.9
mg/L
2.2135
A4500F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09G80429
Water 25mg/L nitrite #1
N02
24.1
mg/L
4.10625
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB09080430
Water 25mg/L nitrite #2
N03
2.6
mg/L
2.2135
A450OF
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080430
Water 25mg/L nitrite #2
N02
25.2
mg/L
4.10625
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB09080431
Water 25rng/L nitrite #3
N03
<0.5
mg/L
2.2135 II
A4500F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080431
Water 25mg/L nitrite #3
N02
25.2
mg/L
4.10625
A4500B
08/00/2009
08/09/2009 Jl
9/8/2009
Jl
HBO9080432
Water 25mg/L nitrite #4
N03
<0.5
mg/L
2.2135 U
A4500F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080432
Water 25mg/L nitrite #4
N02
26.7
mg/L
4.10625
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB09080433
Water 50mg/L nitrite #1
N03
7.3
mg/L
8.854 J
A45Q0F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HBQ9D80433
Water 50mg/L nitrite #1
N02
51.6
mg/L
8.2125
A4500B
08/09/2009
08/09/2009 JI
9/8/2009
Jl
H809080434
Water 50mg/L nitrite #2
N03
8.3
mg/L
8.854 J
A4500F
11/09/2009
06/09/2009 Ben Cook
9/11/2009
Ben Cook
HB09080434A
Wafer 50mg/L nitrite #2
N02
52.0
mg/L
8.2125
A4500B
08/09/2009
08/09/2009 JI
9/8/2009
Jl
HB09080434B
Water 50mg/L nitrite #2
N02
49.6
mg/L
8.2125
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HBO9080435
Water 50rng/L nitrite #3
N03
9.9
mg/L
8.854
A4500F
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HBO9080435
Water 50mg/L nitrite #3
N02
51.1
mg/L
8.2125
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
H609080436
Water 50mg/L nitrite #4
N03
7.5
mg/L
8.854 J
A4SOOF
11/09/2009
08/09/2009 Ben Cook
9/11/2009
Ben Cook
HBQ908Q436
Water 50 mg/L nitrite #4
N02
50.3
mg/L
8.2125
A4500B
08/09/2009
08/09/2009 Jl
9/8/2009
Jl
HB09080437R
Water lOOmg/L nitrite #2
N03
<3
mg/L
11.0675 U, HT
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promeias (Fathead Minnow)
Nominal Nitrite Test Concentration Dilution Water/Control
Sample
Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
no2
no3
NOz
no3
no2
no3
no2
no3
08/03/09
-2
0.0128
1.187
0.030
1.215
<0.0036
1.237
0,0329
1.160
1.160
08/04/09
-1
0.0145
1.089
0.0102
1.256
0.0181
1.048
0.1133
0.860
0.0115
1.067
08/07/09
0
0.0151
1.028
0.0161
1.082
0.0141
1.084
0.0200
1.035
1.085
0.0200
08/10/09
3
0.0145
0.953
08/12/09
5
0.0205
0.969
08/14/09
7
<0.0036
1.097
1.116
08/17/09
10
0.0237
1.068
08/19/09
12
0.0088
1.104
08/21/09
14
0.0140
1.097
1.088
08/24/09
17
<0.0036
1.052
08/26/09
19
0.0247
1.343
08/27/09
20
08/28/09
21
0.0048
1.327
08/31/09
24
0.0079
1.247
1.147
09/01/09
25
09/02/09
26
<0.0036
1.252
1.227
09/04/09
28
0.0096
1.137
1.129
09/08/09
32
0.0049
1.144
0.0125
1.125
0,0082
1.037
0.0232
1,126
Mean 0,0108 1.1197 0.0153 1.1293 0.0068 1.1278 0.0334 1.1073
Min 0.0048 0.9530 0.0079 0.9690 <0.0036 1.0370 0.0096 0,8600
Max 0,0151 1.3270 0,0300 1.2560 0.0181 1.2520 0.1133 1.3430
Mean of all N02 0.0171
Min of all N02 <0.0036
Max of all N02 0.1133
Mean of all NOs
Min of all NOs
Max of all NO,
1.1216
0.8600
1.3430
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promeias (Fathead Minnow)
Nominal Nitrite Test Concentration 6.25 mg/L
Sample Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
no2
no3
no2
no3
no2
no3
no2
no3
08/03/09
-2
5.01
2.1
5.01
1.6
5.11
1.5
4,93
3.5
5.06
08/04/09
-1
4,89
0,85
5.00
0.55
5.00
0.27
4.78
1.11
08/07/09
0
5.7
0.77
5.8
0.93
7,0
<0.11
6.5
<0,11
08/10/09
3
5.22
<0.21
08/12/09
5
08/14/09
7
5.7
1,32
08/17/09
10
08/19/09
12
7.93
0.44
08/21/09
14
08/24/09
17
8.1
0.72
08/26/09
19
08/27/09
20
2.3
1.2
2,4
08/28/09
21
5,0
1.172
5.3
08/31/09
24
09/01/09
25
2,9
1,41
1,28
09/02/09
26
6.4
0.33
09/04/09
28
09/08/09
32
6,7
0.61
6.0
1.73
7.6
<0.11
6.6
0.13
6.8
Mean 4.7244 0.9590 5,2586 1,1438 6,5280 0.4660 6.1100 1.1040
Min 2.3000 <0,021 2.9000 0,3300 5.0000 <0 11 4.7800 <0.11
Max 6.7000 2.1000 6.4000 1.7300 7.9300 1.5000 8.1000 3.5000
Mean of all N02 5.5264
Min of all N02 2.3000
Max of all NO; 8.1000
Mean of all NO,
Min of all N03
Max of all NQ3
0 9485
<0.021
3.5000
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
Nominal Nitrite Test Concentration 12,5 mg/L
Sample Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
no2
no3
no2
no3
no2
no3
no2
no3
08/03/09
-2
10.4
3.2
9.9
1,4
10.0
2.8
10.0
3.1
08/04/09
-1
10.2
<0.21
10,4
<0.21
9.6
0.51
10.3
<0.21
10.6
<0.21
08/07/09
0
11.3
0,37
10.0
1.75
11,0
1.0
12,4
<0,21
08/10/09
3
08/12/09
5
13.8
<0.42
08/14/09
7
08/17/09
10
14.1
<0.5
14.7
<0.5
08/19/09
12
08/21/09
14
13,2
2.09
08/24/09
17
08/26/09
19
13.1
<0.21
08/27/09
20
4.6
1
08/28/09
21
08/31/09
24
9.3
1.57
9,2
09/01/09
25
10.6
<0.21
09/02/09
26
09/04/09
28
13.5
1.05
14.1
09/08/09
32
12,3
1.69
10.7
2.94
12.6
0.93
13.7
<0.21
Mean 10-1375 1,1643 11,4857 0 9729 12.0000 1.3967 11.6833 0.6083
Min 4.6000 <0.21 9.9000 <0,21 9.6000 0.5100 10.0000 <0.21
Max 13,8000 3.2000 14,7000 2.9400 14.1000 2.8000 13.7000 3.1000
Mean of all N02 11 2714
Min of all NOa 4.6000
Max of ail N02 14.7000
Mean of all N03
Min of all NOs
Max of all NOs
1,0381
<0.21
3,2000
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promeias (Fathead Minnow)
Nominal Nitrite Test Concentration 2S.0 mg/L
Sample Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
no2
no3
no2
no3
no2
no3
no2
NO3
08/03/09
-2
194
<0.5
20.6
<0.5
20,0
<0.5
20.8
<0.5
08/04/09
-1
21.7
0,9
21.7
<0.5
23,4
<0.5
21.8
<0.5
08/07/09
0
23.6
1.8
24.6
1.8
20,3
4.3
24.2
<0.5
23.1
2.8
08/10/09
3
24.0
<1.1
08/12/09
5
08/14/09
7
19.0
6.1
08/17/09
10
08/19/09
12
27.0
<0.5
08/21/09
14
08/24/09
17
26,4
2.3
08/26/09
19
08/27/09
20
9.3
2.4
08/28/09
21
22.2
<0.5
08/31/09
24
09/01/09
25
22.2
<0.5
09/02/09
26
25.8
0.7
09/04/09
28
09/08/09
32
24.1
3.9
25.2
2.6
25.2
<0,5
26.7
<0.5
Mean 20.9250 1.8000 22.7286 1.9500 23.1800 1.2625 23.9800 0.7625
Min 9.3000 <0.5 19.0000 <0.5 20.0000 <0.5 20.8000 <0.5
Max 24.1000 3.9000 25 8000 6.1000 27,0000 4.3000 26.7000 2.3000
Mean of all NOz 22.4920
Min of all NOa 9.3000
Max of all N02 27.0000
Mean of all NOj 1.5429
Min of all NOj <0.5
Max of all N03 6.1000
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales prometas (Fathead Minnow}
Nominal Nitrite Test Concentration 50.0 rng/L
Sample
Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
no2
no3
no2
no3
no2
no3
no2
no3
08/03/09
-2
42.4
<1.1
42.2
2,2
56.5
<11
47.1
<1.1
43.9
2,7
08/04/09
-1
48.5
<1.1
46.6
<1,1
49.2
<1.1
50,1
<1.1
08/07/09
0
53.7
4.2
49.5
1.8
52.2
6
54.5
2.1
08/10/09
3
08/12/09
5
57.6
<1.3
57.1
08/14/09
7
08/17/09
10
60,8
<1.1
08/19/09
12
08/21/09
14
49.2
<1.1
08/24/09
17
08/26/09
19
48.8
<1.1
08/27/09
20
15.2
6.6
08/28/09
21
08/31/09
24
44.5
2.2
09/01/09
25
49,2
4.7
09/02/09
26
09/04/09
28
55.6
4.0
09/08/09
32
51.6
7.3
52
8.3
51.1
9.9
50.3
7.5
49.6
Mean 46.3250 3.5833 49,2250 3.3750 52.3000 5.1900 50.1600 2.6750
Min 15.2000 <1.1 42.2000 <1.1 49.2000 <1.1 47.1000 <1.1
Max 57.6000 7.3000 60.8000 8.3000 56.5000 9.9000 54.5000 7.5000
Mean of all NO, 49.2222
Mln of all NO, 15.2000
Max of all NO, 60,8000
Mean of all NOs
Min of all N03
Max of all N03
3.4976
<1.1
9.9000
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
Nominal Nitrite Test Concentration 100.0 mg/L
Sample
Date
Test Day
Replicate 1
Replicate 2
Replicate 3
Replicate 4
no2
no3
no2
no3
no2
no3
no2
no3
08/03/09
-2
167
<11
92
8
69
35
104
<3
170
08/04/09
-1
106
4.4
97
3
100
<3
98
<3
98
3
08/07/09
0
96
<3
82
<3
90
26.6
94
15.5
90
08/10/09
3
51
56
08/12/09
5
08/14/09
7
113
10.3
08/17/09
10
08/19/09
12
139
<2,7
08/21/09
14
08/24/09
17
107
<1.1
08/26/09
19
08/27/09
20
35
<2.1
08/28/09
21
67
3.1
08/31/09
24
09/01/09
25
97
14.4
09/02/09
26
106
<3
102
09/04/09
28
09/08/09
32
103
<3
108
Mean 98.8571 14.0200 99,8000 7.7400 97.6000 20.9833 100.7500 8,0250
Min 35.0000 <1.1 82.0000 3.0000 69.0000 <2.7 94.0000 <1.1
Max 170.0000 56.0000 113,0000 14.4000 139.0000 35.0000 107.0000 15.5000
Mean of all NOz 99.2692
Min of all N02 35.0000
Max of all N02 170.0000
Mean of all NOj 12.5200
Min of all N03 <1.1
Max of all NOa 56.0000
-------
Measured Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
Nominal Nitrite Test Concentration Stock Solutions
Sample
Date
Test Day
Super Stock 32g/l_
NaN02
Stock s
3.2g/L
olution
NaN02
Stock s
2.1 g/L
olution
NaN02
no2
no3
no2
no3
NOj
no3
08/03/09
-2
23400
<500
1880
<30
08/04/09
-1
08/07/09
0
08/10/09
3
08/12/09
5
08/14/09
7
2450
<50
08/17/09
10
08/19/09
12
2320
<530
08/21/09
14
08/24/09
17
08/26/09
19
08/27/09
20
08/28/09
21
1900
<50
08/31/09
24
09/01/09
25
09/02/09
26
2360
<27
09/04/09
28
09/08/09
32
1940
<27
Mean
Min
Max
2194 68.4
1900 <27
2450 <530
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
G'li-C
Page of
739 Hastings St.
Traverse City, MI 49686
Phone; 231-941-2230
Fax: 231-941-2240
www.elec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/3/09
Mailee Garton/Jamie Saxton
Check the requested analysis (see definitions below)
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information I
sample type)
Preservation
Alkalinity
«
Q
O
09
Chloride
Chlorophyll a
tfi
¦ '
• : ;G'
Ui -
Z
W
z.
•A
I
. - -
£<•'
;:I
NO2W-0A-1
8/3/09
125 mL
HDPE
Water control Tank #1
Cold
X
NO2W-0A-2
8/3/09
125 ml.
HDPE
Water control Tank#2
Cold
V
X
NO2W-0A-3
8/3/09
125 mL
HDPE
Water control Tank #3
Cold
¦; v v';:"
X' ¦
NO2W-0A-4
8/3/09
125 mL
HDPE
Water control Tank #4
Cold
X ¦
NO2W-0A-5
8/3/09
125 mL
HDPE
Water 6.25 mg/L
nitrite Tank #1
Cold
X .
NO2W-0A-6
8/3/09
125 mL
HDPE
Water 6.25mg/L
nitrite Tank #2
Cold
X
Shipper:
Date/Time:
Receiver;
Date/Time:
¦? o">
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NO3/NO2 -N = Nitrate/Nitrite-1
Definitions ¦
TDS = Total Dissolve Solids TP = Total Phosphorus
TKN =» Total Kjeldahl Nitrogen TSS = Total Suspended Solids
Abbreviations fdr otherfrequently requested analyses
NO2 -N = Nitrite Nitrogen Total N = Total Nitrogen
NQj -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GlbC
Page of _
739 Hastings St.
Traverse City. MI 49686
Phone; 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/3/09
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below'
Alkalinity
*%
a
o
m
Chloride
Chlorophyll a
7-—T.-'V-'/.j
Hardness ;
£
fO
s
£
1
&
H
- ::a>;
*«¦(
u
¦ ->*¦*.
".'2T
N02W-QA-7
mm
125 niL
HOPE
Overlying water Tank
#3 6.25mg/L nitrite
Cold
x
NO2W-0A-8
8/3/09
125 mL
HDPE
Overlying water Tank
#4 6.25 mg/L nitrite
Cold
x ::
NO2W-0A-9
8/3/09
125 mL
HDPE
Overlying water Tank
#1 12.5 mg/L nitrite
Cold
- . :
x;:/A
.v.-
•. "v
NO2W-0A-10
8/3/09
125 mL
HDPE
Overlying water Tank
#2 12.5 mg/L nitrite
Cold
x
'
NO2W-0A-11
8/3/09
125 mL
HDPE
Overlying water Tank
#3 12.5mg/L nitrite
Cold
Ijr
x; -
-
NO2W-0A-12
8/3/09
125 mL
HDPE
Overlying water Tank
#4 12.5mg/L nitrite
Cold
r '
—
X-V
¦¦ sW '
r:M
Shipper:
Date/Time:
Receiver:
J
Date/Time:
Hoi
Temperature:
=
BODs = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NH3 -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NOj/NOi -N = Nitrate/Nitrite-Nitrogen
ABBreviations for other frequently requested analyses
NOa -N = Nitrite Nitrogen Total N - Total Nitrogen
N03 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Page of
t LC<" I'".-
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
T ,» Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/3/09
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below'
' ¦£*
C
"<3
*n
a
0
»
Chloride
Chlorophyll a
' -«J- - •
, ¦ ;t»- =
V-: G\
• fc*•
z
£
z
Vz-
1 '
:
O
Z
«*>
O
z
w
a
I"
&
t-1
. ,'xn'.
s. t/i •
. H
; .-a:.
- -"¦*» •
-
N02W-GA-I3
8/3/09
125 mL
HOPE
Overlying water Tank
#1 25mg/L nitrite
Cold
XV
NO2W-0A-14
8/3/09
125 mL
HOPE
Overlying water Tank
#2 25mg/L nitrite
Cold
•
X
NO2W-0A-15
8/3/09
125 mL
HDPE
Overlying water Tank
#3 25mg/L nitrite
Cold
w&:-
xv*
NO2W-0A-16
8/3/09
125 mL
HOPE
Overlying water Tank
#4 25mg/L nitrite
Cold
XV ¦
NO2W-0A-17
8/3/09
125 mL
HDPE
Overlying water Tank
#1 50mg/L nitrite
Cold
X/..'
NO2W-0A-18
8/3/09
125 mL
HDPE
Overlying water Tank
#2 50mg/L nitrite
Cold
I
W" ¦
Shipper;
Date/Time:
Receiver:
Date/Time:
g-j ol
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS - Total Suspended Solids
Abbreviations tor otlitr frequently requested analyses
NOi -N = Nitrite Nitrogen Total N - Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
(_
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of
Vj:
J
:"x_-
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax; 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/3/09
Mailee Gartori/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site Information /
sample type)
Preservation
Check the requested analysis (see definitions below
"
Alkalinity
¥3
C
o
ca
Chloride
Chlorophyll a
- - sfl-
- ¦,
-------
CHAIN OF CUSTODY RECORD
I'd a
j3w
Page
of
(To be completed and submitted with samples)
739 Hastings St.
Traverse City, Ml 49686
Phone: 231-941-2230
Fax; 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/3/09
Mailee Garten/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below)
Alkalinity
G
O
CO
Chloride
Chlorophyll a
Hardness
Z
cs
S
z
if"
o.
-sg •
; ft ¦'
: Q.'
TDS
. :h
PL,
H
CO
.CO.
H "
Nitrite
Nitrate
J
NO2W-0A-25
8/3/09
125 raL
HDPE
Water control Tank #1
h2so4
X
NO2W-0A-26
8/3/09
125 mL
HOPE
Water control Tank#2
h2so4
_T ¦¦ -
X
:'
NO2W-0A-27
8/3/09
125 mL
HDPE
Water control Tank #3
h2so4
X
NO2W-0A-28
8/3/09
125 mL
HDPE
Water control Tank #4
h2so4
X
NO2W-0A-29
8/3/09
125 mL
HDPE
Water 6.25 mg/L
nitrite Tank #1
h2so4
X
NO2W-0A-30
8/3/09
125 mL
HDPE
Water 6.25mg/L
nitrite Tank #2
h2so4
X
Shipper:
Date/Time:
Receiver:
A_y
2.
Ay. OJ
Date/Time:
y-3-oT
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NOj/NO^-N = Nitrate/Nitrite-Nitrogen
Definitions -
I DS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other ffeouently requested analyses
NOj -N - Nitrite Nitrogen Total N = Total Nitrogen
NOj -N — Nitrate Nitrogen SKP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of
C-ieai E<
739 Hastings St,
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/3/09
Mailee Garten/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below1
Alkalinity;
V)
Q
O
ea
Chloride.
Chlorophyll a
VJ. .
" -ST .
• •••
z
¦Pi
X
z
.
o..
Z?- .
. tfi .
-¦o:'
CO
c
H
•H •
a.
H
CO
tfl
H
¦9i,
Nitrate
NO2W-0A-31
8/3/09
125 mL
HOPE
Overlying water Tank
#3 6.25mg/L nitrite
h2so4
X
NO2W-0A-32
8/3/09
125 mL
HOPE
Overlying water Tank
#4 6.25 mg/L nitrite
h2so4
-
3
X
NO2W-0A-33
8/3/09
125 mL
HDPE
Overlying water Tank
#1 12.5 mg/L nitrite
h2so4
X
. -
N02W-QA-34
8/3/09
125 mL
HOPE
Overlying water Tank
#2 12.5 mg/L nitrite
hzso4
X
NO2W-0A-35
8/3/09
125 mL
HDPE
Overlying water Tank
#3 12.5mg/L nitrite
h2so4
X
N02W-GA-36
8/3/09
125 mL
HDPE
Overlying water Tank
#4 I2.5mg/L nitrite
h2so4
>, . v
X
Shipper:
Date/Time:
Sfsfol
Receiver:
Ar nJ
Date/Time:
Temperature:
BODj — Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NOj/NOz -N = N itrate/Nitrite-Ni trogen
Definitions
TDS = Total Dissolve Solids TP = Total Phosphorus
TKN " Total Kjeldabl Nitrogen TSS - Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
N05 -N - Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GlbC
Page
of
739 Hastings St,
Traverse City, MI 49686
Phone: 231-941-2230
Fax:231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/3/09
Mailee Garton/Jamie Saxton
Bottle ID
Date! Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
V3
O
O
as
< hlor de
Chlorophyll a
- ift
-8n
® ,
c .
ra:
lm .
«
z
<*>
33
z
z, .
¦ •'
¦' 0
¦*'
rf>
O
z::
V)
0
[m*
' tk'
6-
H
m
- CO/:
: '.H:
** .
' J"? - -
Nitrate
NO2W-0A-37
8/3/09
125 mL
HDPE
Overlying water Tank
#1 25mg/L nitrite
H2SO4
X
NO2W-0A-38
8/3/09
125 mL
HDPE
Overlying water Tank
#2 25mg/L nitrite
H2SO4
X
NO2W-0A-39
8/3/09
125 mL
HDPE
Overlying water Tank
#3 25mg/L nitrite
H2S04
X
NO2W-0A-40
8/3/09
125 mL
HDPE
Overlying water Tank
#4 25mg/L nitrite
h2so4
X
NO2W-0A-41
8/3/09
125 mL
HDPE
Overlying water Tank
# 1 50mg/L nitrite
h2so4
X
NO2W-0A-42
8/3/09
125 mL
HDPE
Overlying water Tank
#2 50mg/L nitrite
H2S04
X
Shipper:
Date/Time:
7
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of
I Pi
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/3/09
Mai lee Garton/Jamie Saxton
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below1
! Alkalinity
w
o
o
«
Chloride
Chlorophyll a
i3
z
%
z
Z-''
1
«
o
z
«-
o
.y;Z:A.
CO
Q
i-
' H '
a.
f-
m ¦
;«3.
K
%
j Nitrate
NO2W-0A-43
8/3/09
125 mL
HDPE
Overlying water Tank
#3 50mg/L nitrite
h2so4
, ..
X
NO2W-0A-44
8/3/09
125 mL
HDPE
Overlying water Tank
#4 50mg/L nitrite
h2so4
... ,
' r •:
X
NO2W-0A-45
8/3/09
125 mL
HDPE
Overlying water Tank
#1 lOOmg/L nitrite
h2so.,
i-.-
X
NO2W-0A-46
8/3/09
125 mL
HDPE
Overlying water Tank
#2 lOOmg/L nitrite
h2so4
1**2!
X
N02W-QA-47
8/3/09
125 mL
HDPE
Overlying water Tank
#3 1 OOmg/L nitrite
h2so4
X
NO2W-0A-48
8/3/09
125 mL
HDPE
Overlying water Tank
#4 lOOmg/L nitrite
h2so4
•.F '
X
Shipper:
Date/Time:
Receiver:
^1/
Date/Time:
Temperature:
Definitions
BODj - Biochemical Oxygen Oemand-5 day TDS - Total Dissolve Solids TP = Total Phosphorus
NHj-N = Ammonia-Nitrogen TKN = Total Kjeldaht Nitrogen TSS - Total Suspended Solids
NOj/NOj -N - Nitrate/Nitrite-Nitrogen
¦
Abbreviations for other frequently reguested i
N02-N = Nitrite Nitrogen Total N = Toted Nitrogen
NO, -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
f '' T T-<( '
V..fLrA.
•: icnt £<
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.eiec.com
Page
of
Great Lakes Environmental Center
Facility:
T Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
5/3/09
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below'
Alkalinity
«
o
PS
Chloride
1
Chlorophyll &
i
«5-: -
v .. ,Vi:
• -SS'
• "o :
-im ¦ ¦
:• a
z
X
z
z.
w
HZ ¦
.©
'Z
to
©
H
¦
0.
H
CO
.60.
H
. Nitrite
| Nitrate
N02W-GA-49
8/3/09
125 mL
HDPE
Steele saWi rw
Cold
X
NO2W-0A-50
8/3/09
125 mL
HOPE
S'Ve C-t—
Cold
v
X
NO2W-0A-51
8/3/09
125 mL
HDPE
^VoUk—
ah^ls.^0^
h2so4
X
NO2W-0A-52
8/3/09
125 mL
HDPE
5>VocJt—
"3,"Uj[c fik.t&X
h2so4
X
D
!P, •
Shipper:
QjjJ- ^"5
2.
air
Date/Time:
BODj = Biochemical Oxygen Deniand-5 day
NHj -N - Ammonia-Nitrogen
NQj/NOi -N = Nitrate/Nitrite-Nitrogen
Receiver:
Date/Time:
Temperature:
Definitions
TDS - Total Dissolve Solids
TKN - Total Kjeldahl Nitrogen
TP - Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOi -N - Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Facility:
Location:
Page
J of?.
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/4/09
Mailee Garton/Jamie Saxton
1 Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below1
=
Alkalinity .
in
Q
C
«
j.
is
, U
Chlorophyll a
rl:
z
S3
z
P
1
Qm
H
qo
. m
H
<6
*£
• *«-< " '
INO2W-0B-1
8/04/09
125 mL
HDPE
Water control Tank #1
Cold
X ..
NO2W-0B-2
8/04/09
125 ml.
HOPE
Water control Tank#2
Cold
i;
•X :
NO2W-0B-3
8/04/09
125 mL
HDPE
Water control Tank #3
Cold
c.
X
;;
:
NO2W-0B-4
S/04/09
125 mL
HDPE
Water control Tank #4
Cold
r.,/;LT5.>v'ir
X . ..
NO2W-0B-5
8/04/09
125 mL
HDPE
Water 6.25 mg/L
nitrite Tank #1
Cold
x.:;
NO2W-0B-6
8/04/09
125 mL
HDPE
Water 6.25mg/L
nitrite Tank #2
Cold
X '
¦¦¦¦
Shipper:
yjnnJL/dfikj
2.
Date/Time:
tMcfi Ibob
Receiver:
£
Date/Time:
Temperature:
Definitions
BODj = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS= Total Suspended Solids
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Abbreviations for other frequently i
N02 -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
r1, itT"'
Page
?orK
^39HsSSn^St."
Traverse City, Ml 49686
Phone: 231-941-2230
Fax; 231-941-2240
www.glec.com
Facility:
Great Lakes Environmental Center
, . Traverse City, MI
Location: '
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/4/09
Mai lee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis {see definitions below"
| Alkalinity
D
s
Chlorophyll a
tr
W3
z:
i
M
s
z
=¦
' ©
£ -
J
m
Q
fr-
-
0.
H
en -1
m-r
¦¦p.
: -
NO2W-0B-7
8/04/09
125 mL
HOPE
Overlying water Tank
#3 6.25mg/L nitrite
Cold
5T:
c;.
NO2W-0B-8
8/04/09
125 mL
HDPE
Overlying water Tank
#4 6,25 mg/L nitrite
Cold
X
NO2W-0B-9
8/04/09
125 mL
HDPE
Overlying water Tank
#1 12.5 mg/L nitrite
Cold
-y.
X ;
NO2W-0B-10
8/04/09
125 mL
HDPE
Overlying water Tank
#2 12.5 mg/L nitrite
Cold
x
NO2W-0B-11
8/04/09
125 mL
HDPE
Overlying water Tank
#3 12.5mg/L nitrite
Cold
X
NG2W-0B-12
8/04/09
125 mL
HDPE
Overlying water Tank
#4 12,5mg/L nitrite
Cold
Shipper:
Date/Time:
Receiver;
2.
Date/Time:
Temperature:
^r
rrrrr
BOD5 = Biochemical Oxygen Demand-5 day TDS - Total Dissolve Solids TP = Total Phosphorus
Nth -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS= Total Suspended Solids
NOj/NO), -N = Nitrate/Nitrite-Nitrogen
Abbreviations for f
NOi-N = Nitrite Nitrogen
NOj -N = Nitrate Nitrogen
Total N = Total Nitrogen
SEP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
vjLEL.
Page
Soft
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/4/09
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalinity
Vi
a
o
oa
Chloride
es
5.
t
6
Hardness
¦ :
z
33
Z
.X
©
T.
~m
O
z
W
a
H
/W*
&
r m -
¦
•/?£.
NO2W-0B-13
8/04/09
125 mL
HDPE
Overlying water Tank
#1 25mg/L nitrite
Cold
¦"7./.
•
X
NO2W-0B-14
8/04/09
125 mL
HOPE
Overlying water Tank
#2 25mg/L nitrite
Cold
>'J
X
NO2W-0B-15
8/04/09
125 mL
HDPE
Overlying water Tank
#3 25mg/L nitrite
Cold
if-
; 2g®»
x ¦
A'--
NO2W-0B-16
8/04/09
125 mL
HDPE
Overlying water Tank
#4 25mg/L nitrite
Cold
x.- ¦
NO2W-0B-17
8/04/09
125 mL
HDPE
Overlying water Tank
#1 50mg/L nitrite
Cold
*?:¦ •
p--.. V
"
JC-,.
NO2W-0B-18
8/04/09
125 mL
HDPE
Overlying water Tank
#2 50mg/L nitrite
Cold
r;
X. -
Shipper:
Date/Time:
jjybJfO fr/q/o'i
Receiver:
& .to"?
Date/Time:
S'S-ol
Temperature:
:: :t:- r; '• ^—" —
Definitions
"v-vr - :
BODj = Biochemical Oxygen Demand-5 day
TDS = Total Dissolve Solids
TP = Total Phosphorus
NHj -N = Ammonia-Nitrogen
TKN = Total Kjeldahl Nitrogen
TSS= Total Suspended Solids
NQj/MOj -N - Nitrate/Nitrite-Nitrogen
Abbrcviattbas for otherfrtaueiitiv requested i
NOj -N - Nitrite Nitrogen Total N = Total Nitrogen
N03 -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Envttuur-'w.-dLa8
739 Hastings St
Traverse City, MI 49686
Phone: 231-941-2230
Fax:231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
h of y
8/4/09
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
Q
O
03
Chloride
Chlorophyll a
Hardness
z
35
Z
% '
©
- ¦
s«
. o
. ..<£
(ZS
a
H
\ K :
h
H
w ¦'
03
'H .
¦ .+>*
:gv
V7- ;!
NO2W-0B-19
8/04/09
125 mL
HDPE
Overlying water Tank
#3 50mg/L nitrite
Cold
X
NO2W-0B-2G
8/04/09
125 mL
HDPE
Overlying water Tank
#4 50mg/L nitrite
Cold
'•S-- ^
TV, '.7. *
X
NO2W-0B-21
8/04/09
125 mL
HDPE
Overlying water Tank
#1 I OOmg/L nitrite
Cold
;:/
X
NO2W-0B-22
8/04/09
125 mL
HDPE
Overlying water Tank
#2 1 OOmg/L nitrite
Cold
^ '
X .
NO2W-0B-23
8/04/09
125 mL
HDPE
Overlying water Tank
#3 1 OOmg/L nitrite
Cold
r--" V:
-
-
X ¦
NO2W-0B-24
8/04/09
125 mL
HDPE
Overlying water Tank
#4 1 OOmg/L nitrite
Cold
•-
X
_'
Shipper: Date/Time: Receiver: Date/Time: Temperature:
dMk &oo ft'J-cA
2.
Definitions
BODj - Biochemical Oxygen Demand-S day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS - Total Suspended Solids
NOj/NOj -N - Nitrate/Nitrite-Nitrogen
v..., ...... . ........
' Abbreviations for other frequently requested analyse*
NQ2 -N = Nitrite Nitrogen Total N = Total Nitrogen
NOs -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page ^ of ^
739 Hastings St
Traverse City, MI 49686
Phone: 231-941-2230
Fax; 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/4/09
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
' Alkalinity
««
O
O
ea
| Chloride
i -
Chlorophyll a
¦ m
-fie; ^
,• «
'.yt-ji: -
- .-or.
OS
S:
2
X
z
' %
- 0:.
O
TDS
a,
H
' (/)
M .
¦ +*-' ¦
.x:-
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"me
z:
Nitrate
NO2W-0B-25
8/04/09
125 ml.
HDPE
Water control Tank #1
l-LSO.,
X
NO2W-0B-26
8/04/09
125 mL
1-lDPE
Water control Tank#2
h2so4
'
X
NO2W-0B-27
8/04/09
125 mL
HDPE
Water control Tank #3
H2SO4
X
NO2W-0B-28
8/04/09
125 mL
HDPE
Water control Tank #4
h?so4
X
NO2W-0B-29
8/04/09
125 mL
HDPE
Water 6.25 mg/L
nitrite Tank#l
h2so4
X
NO2W-0B-30
8/04/09
125 mL
HDPE
Water 6.25mg/L
nitrite Tank #2
h2so4
X
Shipper:
Date/Time;
fl/v/al (loOn
Receiver:
A^oL
Date/Time;
Temperature:
BODs = Biochemical Oxygen Deihand-S day
NH3 -N - Ammonia-Nitrogen
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen
' r Definitions
TDS = Total Dissolve Solids TP = Total Phosphorus
TKN - Total Kjeldahl Nitrogen TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECOED
(To be completed and submitted with samples)
GlbC.
Page of
y t
'.jBit Er.vi
739 Hastings St,
Traverse City, MI 49686
Phone: 23]-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/4/09
Mailee Garton/Jamie Saxton
Bottle IB
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below
Alkalinity
a
o
«
Chloride
Chlorophyll a
z
X
z
C/3
Q
H
TKN
IX
H
OS
CO
0*
•tS- .
u
+*
c«
U
z
NO2W-0B-31
8/04/09
125 ml.
HDPE
Overlying water Tank
#3 6.25mg/L nitrite
h2so„
J:^|
X
NO2W-0B-32
8/04/09
125 mL
HDPE
Overlying water Tank
#4 6.25 mg/L nitrite
h2so4
r
X
NO2W-0B-33
8/04/09
125 mL
HDPE
Overlying water Tank
#1 12.5 mg/L nitrite
h2so4
¦
i ¦
i'. '
X
NO2W-0B-34
8/04/09
125 mL
HDPE
Overlying water Tank
#2 12.5 mg/L nitrite
h2so.,
' •
X
N02W-OB-35
8/04/09
125 mL
HDPE
Overlying water Tank
#3 12.5mg/L nitrite
h2so4
X
NO2W-0H-36
8/04/09
125 mL
HDPE
Overlying water Tank
#4 12.5mg/L nitrite
h2so,
X
Shipper:
Date/Time:
%A
Receiver:
Date/Time:
Temperature:
Definitions
BODj = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj -N = Ammonia-Nitrogen TKN - Total Kjeldahl Nitrogen TSS= Total Suspended Solids
HOj/HOz -N - Nitrate/Nitrite-Nitrogen
Abbreviations far other rreqwntlv requested analyses
NOi -N = Nitrite Nitrogen Total N = Total Nitrogen
N£>3 -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page l_
1 off
ri
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax:231-941-2240
www.gkc.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, Ml
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/4/09
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalin tv
vt
0
O
a
¦ »
"jo
Chlorophyll a
•
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'' .'C
• '¦"£ ¦
z
a?
z
*
6
Z.
O
SC
Cfi
O
•p' '
ft*
: S
Z?
.1
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NO2W-0B-37
8/04/09
125 mL
HDPE
Overlying water Tank
#1 25mg/L nitrite
H2SO4
""i ¦
:
1.-
. _
'V. '
X
NO2W-0B-38
8/04/09
125 mL
HDPE
Overlying water Tank
#2 25mg/L nitrite
HjSO,
^
hi.-X
X
NO2W-0B-39
8/04/09
125 mL
HDPE
Overlying water Tank
#3 25mg/L nitrite
h2so4
•
X
NO2W-0B-40
8/04/09
125 mL
HDPE
Overlying water Tank
#4 25mg/L nitrite
H2SO4
>v"
X
•"V. ' -
NO2W-0B^l
8/04/09
125 mL
HDPE
Overlying water Tank
#1 50mg/L nitrite
II2SO4
X
NO2W-0B-42
8/04/09
125 mL
HDPE
Overlying water Tank
#2 50mg/L nitrite
h2so4
?:;v
,sr '•,
X
Shipper:
Date/Time:
¦ /yibidji S/v/ol lh*b
Receiver:
JL^nA.
2.
BOD5 = Biochemical Oxygen Demand-5 day
NHj -N " Ammonia-Nitrogen
NOj/NOj -N Nitrate/Nitrite-Nitrogen
Date/Time:
S--S-
Temperature:
Definitions
TDS - Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP - Total Phosphorus
TSS - Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -N - Nitrite Nitrogen Total N = Total Nitrogen
N03 -N " Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Gle
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
8/4/09
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
_c
'
• J*
ft
0
M
Chloride
Chlorophyll a
. <». ¦-
• -C' .
..at
":(as :
z
X
z
0
z
"rn
0
z
TDS
a-
H
m
-i-v
Nitrite
Nitrate
NO2W-0B-43
8/04/09
125 mL
HOPE
Overlying water Tank
#3 50mg/L nitrite
h2so4
X
1
NO2W-0B-44
8/04/09
125 mL
HOPE
Overlying water Tank
#4 50mg/L nitrite
h2so4
X
N02W-OB-45
8/04/09
125 mL
HDPE
Overlying water Tank
#1 lOOmg/L nitrite
h2so4
X
NO2W-0B-46
8/04/09
125 mL
HDPE
Overlying water Tank
#2 lOOmg/L nitrite
h2so4
X
NO2W-0B-47
8/04/09
125 mL
HDPE
Overlying water Tank
#3 lOOmg/L nitrite
h2so«
X
NO2W-0B-48
8/04/09
125 mL
HDPE
Overlying water Tank
#4 1 OOmg/L nitrite
h2so4
•f/". yr).
X
Shipper:
2.
Date/Time:
IS&Cb
Receiver:
Date/Time:
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NO3/NO2 -N = Nittate/Nitrite-Nitrogen
Pefinitlbmis , ¦
TDS = Total Dissolve Solids
TKN — Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS - Total Suspended Solids
Abbreviations for other frequently requested analyses
NO,- -N ¦= Nitrite Nitrogen Total N = Total Nitrogen
NOj -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
{To be completed and submitted with samples)
Facility:
Page of
Clear u^-es Ei-'.-irciJrneKVr.!
739 Hastings St
Traverse City, MI 49686
Phone; 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
. . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Bottle ID
£
C
'm
-
V3
O
Q
e
Chloride
Chlorophyll a j
' CO
-Sf}.;
'
'
U
m .
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z
tc
z
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page of
•Zsiem Lriktr-i Cc.iUn
739 Hastings St
Traverse City, MI 49686
Phone; 231-941-2230
Fax: 231-941-2240
www.elec.com
Great Lakes Environmental Center
Facility:
. . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
>
Bottle ID
a
O
«
Chloride
«53
f.
JS
0.
1
JS
u
I-
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. £ •
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SB
Z
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03
a
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Cm
H
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' -OB
¦
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• •
'
, & '
' -'V.:".;
NO2W-0-7
8/7/09
125 ml.
HOPE
Overlying water Tank
#3 6.25mg/L nitrite
Cold
;
X--
: *
NO2W-0-8
8/7/09
125 ml.
HOPE
Overlying water Tank
#4 6.25 rrig/L nitrite
Cold
X-'
\ ;v' -
NO2W-0-9
8/7/09
125 mL
HOPE
Overlying water Tank
#1 12.5 mg/L nitrite
Cold
::
S-V-."=
L" -
f
¦ VvC-."
xyC
NO2W-0-10
8/7/09
125 mL
HDPE
Overlying water Tank
#2 12.5 mg/L nitrite
Cold
:
X .
INO2W-0-11
8/7/09
125 mL
HOPE
Overlying water Tank
#3 12,5mg/L nitrite
Cold
X
NO2W-0-12
8/7/09
125 mL
HDPE
Overlying water Tank
#4 12.5mg/L nitrite
Cold
x
.'
Shipper:
Date/Time:
i
Receiver:
Date/Time:
y-7-oi
Temperature:
BODj = Biochemical Oxygen Demand-5 day
NH? -N - Ammonia-Nitrogen
NO3/NQ2 -N = Nitrate/Nitrite-Nitrogen
Definitions.
TDS = Total Dissolve Solids TP - Total Phosphorar
TKJN = Total Kjeldahl Nitrogen- TSS = Total Suspended Solids
NO2 -N = Nitrite Nitrogen
NOj -N = Nitrate Nitrogen
Total N = Total Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
j'LEk^
Page of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
cxom
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location;
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalinity
P
O
«
| Chloride
Chlorophyll a
Hardness
z
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
fjWC
Page of
Gisjat t-cisw>*»
739 Hastings St.
Traverse City, Ml 49686
Phone:231-941-2230
Fax: 231-941-2240
www.glec.conn
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
&
.5 .
15 '*
BODs
Chloride
Chlorophyll a
Hardness
z
Fi
S3
Z
0
z.
w
a
H
•H
c-
(-¦
Nitrite
V- t
•>.» .
NO2W-0-19
8/7/09
125 mL
HDPE
Overlying water Tank
#3 50mg/L nitrite
Cold
-
V'- '
r ,
X
N02W-0-20
8/7/09
125 mL
IIDPE
Overlying water Tank
#4 50mg/L nitrite
Cold
-
xv:;;
NO2W-0-21
8/7/09
125 mL
HDPE
Overlying water Tank
#1 lOOmg/L nitrite
Cold
: '
«;%
X;v
NO2W-0-22
8/7/09
125 mL
HDPE
Overlying water Tank
#2 lOOrng/L nitrite
Cold
X
NO2W-0-23
8/7/09
125 mL
HDPE
Overlying water Tank
#3 lOOrng/L nitrite
Cold
X
NO2W-0-24
8/7/09
125 mL
HDPE
Overlying water Tank
#4 1 OOmg/L nitrite
Cold
X"'
-
Shipper:
Date/Time:
%bjtCl
Receiver:
(LA
Date/Time:
8-7-0^
Temperature:
2,
- ¦ ¦ 111 1 .. : . 1 • 1 . . ¦ ¦.
Definitions
BODj = Biochemical Oxygen Demand-5 day TDS »= Total Dissolve Solids TP = Total Phosphorus
NH3 -N Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NO3/NO1-N = Nitrate/Nitrite-Nitrogen
NOi -N - Nitrite Nitrogen
NOj -N = Nitrate Nitrogen
Total N = Total Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page of
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location: -
Date.
Contact Person:
Email Address:
Phone Number:
Collector:
Mai lee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
)
'E
- «
<
0
0
PQ
J Chloride
Chlorophyll a
w
Z,
n
a
z.
¥
o _
- • '
¦z
Vl
a
r^
H
¦3 -
<#-*
i-
«p-
NO2W-0-25
8/7/09
125 mL
HOPE
Water control Tank #1
h2so4
•••
..
¦ . r ;
X
NO2W-0-26
8/7/09
125 mL
HDFE
Water control Tank#2
h2so4
X
NO2W-0-27
8/7/09
125 mL
1JDPE
Water control Tank #3
h2so4
'V
,4
X
NO2W-0-28
8/7/09
125 mL
HOPE
Water control Tank #4
h2so4
iti
X
NO2W-0-29
8/7/09
125 mL
HDPE
Water 6.25 mg/L
nitrite Tank #1
h2so.,
X
N02W-0-30
8/7/09
125 mL
HDPE
Water 6.25mg/L
nitrite Tank #2
H2S04
X
Shipper: Date/Time: Receiver: Date/Time: Temperature:
- , v ' " - ' | • ¦ • • ' •• v-
Definitions J Abbreviations for other frequently requested analyses
BODj = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP «= Total Phosphorus N02-N = Nitrite Nitrogen Total N = Total Nitrogen
NH3 -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids N03 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
NOj/NOi -N » Nitrate/Nitrite-Nitrogen
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of
GleC
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.com
Great Lakes Environmental Center
Facility:
. . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
a
0
m
Chloride
Chlorophyll a
Hardness
y..
«
r*>
t—f
Z
sc
r
¦¦ •.*•* ¦'
O
5
> ¦
Pm
jiprt
. ¦ H
- u, -
Nitrate
NO2W-0-31
8/7/09
125 mL
HDPE
Overlying water Tank
#3 6.25mg/L nitrite
H2SO*
X
NO2W-0-32
8/7/09
125 mL
HDPE
Overlying water Tank
#4 6.25 mg/L nitrite
H2SO4
X
NO2W-0-33
8/7/09
125 mL
HDPE
Overlying water Tank
#1 12.5 mg/L nitrite
h2so4
X
NO2W-0-34
8/7/09
125 mL
HDPE
Overlying water Tank
#2 12,5 mg/L nitrite
H2S04
X
NO2W-0-35
8/7/09
125 mL
HDPE
Overlying water Tank
#3 12.5mg/L nitrite
h2so4
X
JNO2W-0-36
8/7/09
125 mL
HDPE
Overlying water Tank
#4 12.5mg/L nitrite
h2so4
X
Shipper:
llyffehOjg/ (a)
2.
Date/Time:
Receiver:
Date/Time:
Temperature:
BQDs = Biochemical Oxygen Demand-5 day
NH3-N = Ammonia-Nitrogen
NO3/NO2-N = Nitratc/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS= Total Suspended Solids
Abbreviations for other frequently requested analyses
NO3 -N - Nitrite Nitrogen Total N = Total Nitrogen
NO3 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page
of
OfeiU i.
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
www.elec.com
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mai lee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
» 1
| Alkalinity
P
O
05
Chloride
Chlorophyll a
Hardness
z
B
Z
i
r
1
&
•'¦H *
' £
&
U
¦+>*
2
3
* *':r
NO2W-0-37
8/7/09
125 mL
HDPE
Overlying water Tank
#1 25mg/L nitrite
h2so4
X
NO2W-0-38
8/7/09
125 mL
HDPE
Overlying water Tank
#2 25mg/L nitrite
h2so4
X
-•
•. . ;>¦
NO2W-0-39
8/7/09
125 mL
HDPE
Overlying water Tank
#3 25mg/L nitrite
H2S04
Xft y:.'-
v,
X
N02W-0-40
8/7/09
125 mL
HDPE
Overlying water Tank
#4 25mg/L nitrite
H2S04
•V,-
X
NO2W-0-41
8/7/09
125 mL
HDPE
Overlying water Tank
#1 50mg/L nitrite
h2so4
X
fj.v ¦¦ '
/1
'
NO2W-0-42
8/7/09
125 mL
HDPE
Overlying water Tank
#2 50mg/L nitrite
h2so4
X
¦"¦r *
Shipper:
L CX-'
2.
BODj = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen .
Date/Time:
Definitions
TDS = Total Dissolve Solids
TKN - Total Kjeldahl Nitrogen
Receiver:
Date/Time:
5 -7-a?
Temperature:
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analysts
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
NOs -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page of_
GiCfit Environments?
739 Hastings St.
Traverse City, MI 49686
Phone; 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailcc Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalinity
Q
O
03
Chloride
Chlorophyll a
Hardness
Z
<*S
z
ss: ¦:
r'
0
it
•0
z.
w
O
H
0.
E-
'¦-'xfi-:
• «¦
'¦ '.'fa;.
' ' "P*/
Nitrate
NO2W-0-43
8/7/09
1 1 1—j
HDPE
Overlying water Tank
#3 50mg/L nitrite
h2so4
X
NO2W-0-44
8/7/09
125 mL
HDPE
Overlying water Tank
#4 50mg/L nitrite
h2so4
X
NO2W-0-45
8/7/09
125 mL
HDPE
Overlying water Tank
#1 lOOmg/L nitrite
I LSO^
X
NO2W-0-46
8/7/09
125 mL
HDPE
Overlying water Tank
#2 lOOmg/L nitrite
H2SO,
X
NO2W-0-47
8/7/09
125 mL
HDPE
Overlying water Tank
#3 lOOmg/L nitrite
HjSO,j
X
NO2W-0-48
8/7/09
125 mL
HDPE
Overlying water Tank
#4 lOOmg/L nitrite
H2SO4
X
Shipper:
l- >~Tw.> for a
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
CjIJA..-
Page
of
739 Hastings St,
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
. Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
TfZj/Uy
Sjiojdl
Mailee Garton/Jamie Saxtori
m. garton \ 3,
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Bottle ID
Alkalinity
W3
Q
0
«
Chlorophyll a
"«9„"
. * " •
C ;
- -W
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
UlEC
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
m.
°aiton J. Anmi j
Date /Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below"
1
Bottle ID
Alkalinity
Q
O
sc
Chloride
Chlorophyll a
Hardness
7-
1
a
z
z
.0
z
0
Z ¦
05
e
H
flu
H
-'B .
+¦»
.-m-
..
•»-« .
N02W-3-7
8/10/09
125 mL
HOPE
overlying water tank # 1
100 mg/L Nitrite -Day 3
h2so4
X
N02W-3-8
8/10/09
125 mL
HDPE
overlying water tank #1
100 mg/L Nitrite -Day 3
Cold
X
Shipper:
Q., ,)
Date/Time:
2.
Receiver:
Date/Time:
Temperature;
BODs = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP - Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NO- -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
CjLeC
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Sli^loq
Mailee Garton/Jamie Saxton
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalinity
a
o
m
-fW~: '
J. '£
U
Chlorophyll a
| Hardness
T.
X
Z
o
Z -
• "**}
o
-25
Cfi
O
i ¦
Bm
H
ce
05.
H
Nitrate J
Nitrite
N02W-5-1
8/12/09
125 mL
HDPE
overlying water tank #2
control Nitrite-Day 5
h2so.,
X
N02W-5-2
8/12/09
125 mL
HDPE
overlying water tank #2
control-Day 5
Cold
X
N02W-5-3
8/12/09
125 mL
HDPE
overlying water tank #1
12.5mg/L Nitrite-Day 5
h2so4
X*
N02W-5-4
8/12/09
125 mL
HDPE
overlying water tank #1
12.5mg/L Nitrite -Day 5
Cold
X
N02W-5-5
8/12/09
125 mL
HDPE
overlying water tank #1
50 mg/L Nitrite -Day 5
h2so4
X
N02W-5-6
8/12/09
125 mL
HDPE
overlying water tank #1
50 mg/L N itrite -Day 5
Cold
X
Shipper:
Date/Time:
?/i3-[on
Receiver:
1U.Q)
Date/Time:
Temperature:
BOD, = Biochemical Oxygen Demand-5 day
NHs -N = Ammonia-Nitrogen
N0j/N02 -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids TP = Total Phosphorus
TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total "N - Total Nitrogen
N03 -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
of.
Great late* Erwlronmwjtai Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
N02W-7-1
N02W-7-2
N02W-7-3
N02W-7-4
N02W-7-5
N02W-7-6
Date / Time
of Sample
8/14/09
J/14/09
8/14/09
8/14/09
8/14/09
J/14/09
Volume
Collected
125 mL
125 ml.
125 niL
125 ml.
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
overlying water tank #3
control Nitrite-Day 7
overlying water tank #3
control-Day 7
overlying water tank #2
6.25mg/L Nitrite-Day 7
overlying water tank #2
6.25mg/L Nitrite -Day 7
overlying water tank #2
25 mg/L Nitrite -Day 7
overlying water tank #2
25 mg/L Nitrite -Day 7
Check the requested analysis (see definitions below)
Preservation
U,S04
Cold
H2SO4
Cold
H7SO4
Cold
Shipper:
L Ck- ^
2,
ROD, = Biochemical Oxygen Deraahd-5 iLiy
= Amnion i a - Nitrogen
NOj/NOj-N = Nitrate/fNitTite-Nitrogen
Date/Time:
IKfinitifiiK
IDS Tnul I .'i* .Solnl"
TKN = Total Kjcldahl Nitrogen
Receiver:
Date/Time:
8'lj -oi
Temperature:
rp = J.Mai Phospt.iru".
TSS = Total Suspended Solids
Ahhrcrraaoift for other trcuuenth rctiueMid •¦iiialw*.
NOj -N = Nitrite Nitrogen 'Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
of
GleC
Great Lakes E/ivifon mental Cemur
739 Hastings St
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Sax ton
m. garton
Bottle ID
N02W-7-7
N02W-7-
N02W-7-9
N02W-7-
10
Date / Time
of Sample
8/14/09
5/14/09
8/14/09
8/14/09
Volume
Collected
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HOPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
overlying water tank #2
100 mg/L Nitrite -Day 7
overlying water tank #2
100 mg/L Nitrite -Day 7
Stock solution
2.1 g/LN02
3-fi/L NaNQ2 - Day 7
Stock solution
2.1 g/L N02
3.g/L NaNQ2 - Day 7
Preservation
h2so4
Cold
H2S04
Cold
Shipper:
L A.; T>p6bccc-e sJe
Date/Time:
Receiver:
Date/Time:
Temperature:
IK IP-, Uun.ht nilgai Oxygin OtnianJ S
NT I3 -N = AmtrKHiia-Nurogen
N(VN02;N = N itiate/N itn (e-Nitrogen
Hfliiiilv«n
IDS - lotil Dwulvc Suliils
TKN = Total K jeldahl Niliugen
'lP-= liJtl]
TSS PutuI Vi^pc: Ji> <-Ylio-.
MibrcriMhons fur other frt-unentlv mim-itc
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
i_of_L
GleC
Great Lake* Environmental Cenis?
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location; J
Date;
Contact Person:
Email Address:
Phone Number:
Collector:
^l\i|hq
Mailee Garton/Jamie Sax ton
m, garton
Bottle ID
NO2W-10-1
NO2W-10-2
NO2W-10-3
NO2W-10-4
NO2W-10-5
NO2W-10-6
Date / Time
of Sample
8/17/09
8/17/09
8/17/09
8/17/09
8/17/09
8/17/09
Volume
Collected
125 mL
125 ml.
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
overlying water tank #4
control Nitrite-Day 10
overlying water tank #4
control-Day 10
overlying water tank #2
12,5mg/L Nitrite-Day 10
overlying water tank #2
12.5mg/L Nitrite -Day 10
overlying water tank #2
50 mg/L Nitrite -Day 10
overlying water tank #2
50 mg/L Nitrite -Day 10
Cheek the requested analysis (see definitions below)
Preservation
h2so4
Cold
h2so4
Cold
h2so4
Cold
1.
2.
Shipper:
Date/Time:
Receiver:
Date/Time:
S ¦17-ov'NOa-N = Nitiatc/Nitfitc-Nitrogen
Abhi t'viatloiu. tor oilier lre
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
Grsai Late* Environmental Corner
739 Hastings St,
Traverse City, MI 496E6
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garten/Jamie Saxtoti
m. garton
Bottle ID
N02W-12-1
N02W-12-2
N02W-12-3
N02W-12-4
N02W-12-5
N02W-12-6
Date /Time
of Sample
8/19/09
3/19/09
8/19/09
8/19/09
8/19/09
8/19/09
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site information /
sample type)
overlying water tank #1
control Nitrite-Day 12
overlying water tank #1
control Nitrite-Day 12
overlying water tank #3
6.25mg/L Nitrite-Day 12
overlying water tank #3
6.25mg/L Nitrite -Day 12
overlying water tank #3
25 mg/L Nitrite -Day 12
overlying water tank #3
25 mg/L Nitrite -Day 12
Check the requested analysis (see definitions below)
Preservation
H?SO,
Cold
H,S04
Cold
h,so4
Cold
Date/Time:
Shipper:
2.
Receiver:
Date/Time:
Temperature-
BO! >i = Biochemical Oxygen Dcmand-S day '
NH3 -N = Ammonia-Nitrogen
NOt/NO; -N = Nitrate/Nitritc-Nitrogen
Dffiaitions
TPS 'I.imI H-.ssoh.. S-.'i.k
TKN = Total Kjetdahl Nitrogen
'IT = Tiidl iTj-j'-'phcuii'.
TSS =¦ Total Suspended Solids
_ Abbreviations for other"frequently rcuuested analyse
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
NO} -N = Nitrate Nitrogen SRI* = Soluble Reacuvi Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
of
Great tntes Efwlfonmentaj Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
, . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
NQ2W-12-
7
N02W-12-
ikTSm-
10
Date / Time
of Sample
8/19/09
3/19/09
Volume
Collected
125 mL
125 mL
l/l
Type of
Sample
Container
HDPE
HDPE
Sample Description
(Site information /
sample type)
overlying water tank #3
100 mg/L Nitrite -Day
12
overlying water tank #3
100 mg/L Nitrite -Day
12
lOft-
Shipper:
Date/Time:
(CFI
Receiver:
^ 0 (J
Date/Time:
$ -x -ct<; ,i>Ke Soln1\
NH, -N = Ammonia-Nitrogen
NO/NO2-N = N i tra I e/N ltritc:- Ni trogen
'1"KN = Total Kjcldahl Nitrogen
TP = Total. Phosphorus I-
1SS - TnL.1 S'Kf/i JSul ls
4Min tutifuis for other freqiu ntlv requests! jmlp.es
NO2 -N = Ni&ire Nitrogen • - Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRI' = Soluble Reactive Phosplioius
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
of
Qn/-1
,EL
<3reat lakas Environmental Center
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
_ . Traverse City, MI
Location:
Date;
Contact Person:
Email Address:
Phone Number:
Collector;
Mai lee Garton/Jamie Saxton
m. garton
Bottle ID
N02W-14-1
N02W-14-2
N02W-14-3
N02W-14-4
N02W-14-5
N02W-14-6
Date I Time
of Sample
8/21/09
8/21/09
8/21/09
8/21/09
8/21/09
8/21/09
Volume
Collected
125 mL
125 mL
125 mL
125 mL
125 mL
125 mL
Type of
Sample
Container
HDPE
HDPE
HDPE
HDPE
HDPE
HDPE
Sample Description
(Site Information /
sample type)
overlying water tank #2
control Nitrite-Day 14
overlying water tank #2
control Nitrite-Day 14
overlying water tank #3
12.5mg/L Nitrite-Day 14
overlying water tank #3
12.5mg/L Nitrite -Day 14
overlying water tank #3
50 mg/L Nitrite -Day 14
overlying water tank #3
50 mg/L Nitrite -Day 14
Shipper;
Date/Time:
Receiver:
A^-0
Date/Time:
8
Temperature:
IJODj = Biochemical Oxygen Demand'-5 day
NHi-N = Ammonia-Nilrogen
NO/NO2 -N = Nitrate/Nitrite-Nilrogen
Definitions
im- Tctallii,su'vc S-i!i'b.
"1KN = Total Kjtildahl Nitrogen
TP = Total Phosphorus
TSS = Tnul Suspended Solids
NO, -N = Nitrite Nitrogen-
NO3 -N = Nitrate Nitrogen
Total N = Total Nitiogcn
SRI' = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GiiiC
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
vvww.glec.com
Great Lakes Environmental Center
Facility:
. Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
O
O
• •-
-33 ;
*E •
-
U
Chlorophyll a
Hardness
T.
X
z
%
,©
z
0 ¦
z
w
a
H
H
ft.
f-
co
«5
£¦«
; Nitrate
Nitrite
N02W-17-1
8/24/09
i 25 mL
HOPE
overlying water tank #3
control Nitrite-Day 17
h2so4
x :
N02W-17-2
8/24/09
125 mL
IJDPE
overlying water tank #3
control Nitrite-Day 17
Cold
X
N02W-17-3
8/24/09
125 mL
HDPE
overlying water tank #4
6.25mg/L Nitrite-Day 17
11, SO.,
X
N02W-17-4
8/24/09
125 mL
HDPE
overlying water tank #4
6.25mg/L Nitrite -Day 17
Cold
X
N02W-I7-5
8/24/09
125 mL
HDPE
overlying water tank #4
25 mg/L Nitrite -Day 17
H2SO4
X
In02W-17-6
8/24/09
125 mL
HDPE
overlying water tank #4
25 mg/L Nitrite -Day 17
Cold
X
Shipper:
Date/Time:
Receiver:
jfiUy j/
Date/Time:
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NOj/NOz-N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS - Total Suspended Solids
Abbreviations for other frequently requested analyses
NOi-N** Nitrite Nitrogen Total N = Total Nitrogen
NO3-N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
739 Hastings St,
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
of
Mai lee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below1
1
¦•¦las ;v':
s
0
Cfi
"C"
¦ '-2
'. S: •
0V .
Chlorophyll a
Hardness
z
1
B
Z
•Z
«
O
Z'
0
z
<»
Q
TKN
Cu
H
m
cc
H
Nitrate
N02W-17-
7
8/24/09
125 mL
HOPE
overlying water tank #4
100 mg/L Nitrite -Day
17
h2so4
X '
N02W-17-
8
8/24/09
125 mL
HOPE
overlying water tank #4
100 mg/L Nitrite -Day
17
Cold
X
Date/Time:
Shipper:
Receiver:
/I-a
Date/Time:
Temperature:
BODs = Biochernical Oxygen Demaitd-5 day
NHj -N = Ammonia-Nitrogen
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS - Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
N03 -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page.
of
739 Hastings St
Traverse City, Ml 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.gjec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
)
Alkalinity j
O
O
CO
Chloride
Chlorophyll a
Hardness
Z
1
s
z
z 7
-as ..
CO
Q
TKN
ft.
H
m
f2
• :*-*.:
¦ t-
•tm
£
J Nitrite
N02W-19-1
8/26/09
125 mL
HOPE
overlying water tank #4
control Nitrite-Day 19
h2so*
X
N02W-19-2
8/26/09
125 mL
HDPE
overlying water tank #4
control Nitrite-Day 19
Cold
X
N02W-19-3
8/26/09
125 mL
HDPE
overlying water tank #4
12.5rng/L Nitrite-Day 19
II2SO,(
X
N02W-19-4
8/26/09
125 mL
HDPE
overlying water tank #4
12.5mg/L Nitrite -Day 19
Cold
X
N02W-19-5
8/26/09
125 mL
HDPE
overlying water tank #4
50 mg/L Nitrite -Day 19
h2so4
X
N 02 W-19-6
8/26/09
125 mL
HDPE
overlying water tank #4
50 mg/L Nitrite -Day 19
Cold
X
Shipper:
Date/Time:
Receiver:
Date/Time:
S-27-o
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Qleu
Page of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.com
Great Lakes Environmental Center
Facility:
T Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below) [
Alkalinity
v<
Q
O
M
Chloride
Chlorophyll a
Hardness
z
s
SK
Z
1
O
%
"M
Z.'
m
a
f-
¦ -F*'
c.
H
V.-wa
to,
H-
Nitrite
NO2W-20-1
&m09 mh"
125 niL
HDPE
Water 6.25 mg/L
nitrite Tank # (
H2S04
X '
NO2W-20-2
mm' ajJ^
125 mL
HOPE
Water 6.25 mg/L
nitrite Tank # |
Cold
~ :
X
NO2W-20-3
S/3#9 (W*
125 mL
HDPE
Overlying water Tank
# I 12.5 mg/L nitrite
H2S04
X:
NO2W-20-4
T|3i(on
125 mL
HDPE
Overlying water Tank
# 112.5 mg/L nitrite
Cold
X
NO2W-20-5
8/3/09 fkiAU
125 mL
HDPE
Overlying water Tank
25mg/L nitrite
H2S04
X
NO2W-20-6
8/3/09 /Hr
125 mL
HDPE
Overlying water Tank
25mg/L nitrite
Cold
X
Shipper:
Date/Time:
Receiver:
Q/f
Date/Time:
<5 ' 2 7 -o*7
Temperature:
Definitions
BODs = Biochemical Oxygen Dcmand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NH3 -N = Ammonia-Nitrogen TKN -¦ Total Kjeldahl Nitrogen TSS = Total Suspcnded .Solids
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen
Abbreviations for other Jreqiientlv requested analyses
NO2 -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Gl
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
>r:,s,
¦ ^5
• jsd:..-
; W-
V3
Q
O
ea
Chloride
Chlorophyll a
to
m
e
- ro-
1m
" -
tt ;
Z
S
z,
O
r
ft
¦O'
¦
SO
a
1-
-f* ¦
p-
m ¦
w
0
."fci -
*rm
z
X
¦+«*
SC
NO2W-20-7
125 tnL
HOPE
Overlying water Tank
# "\ 50mg/L nitrite
H2S04
.. ' :>V
X
NO2W-20-8
125 mL
HOPE
Overlying water Tank
# I 50mg/L nitrite
Cold
X
NO2W-20-9
125 mL
HDPE
Overlying water Tank
# jlOOmg/L nitrite
H2S04
...
3f:'"'
N02W-20-10
125 mL
HOPE
Overlying water Tank
# | lOOmg/L nitrite
Cold
~r . : '
X
i-
u
X
X
Shipper:
Date/Time:
BODj = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NOj/NOj -N - Nitrate/Nittite-Nitrogen
Definitions
TDS - Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
Receiver:
Date/Time:
Temperature:
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NO2 -N = Nitrite Nitrogen Total N = Total Nitrogen
NO3 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of _
739 Hastings St.
Traverse City. MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector: m' Earton
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below
Alkalinity
0
0
«
i
Chlorophyll a
Hardness
2
X
z
z
c
z
O
Z
Vi
G
H
ft.
H
' W
go
f-
»
1. 8t -
'Z
Hi-)
Z
N02W-21-1
8/28/09
125 mL
HOPE
overlying water tank #1
control Nitrite-Day 21
h2so4
. ' : ' •
X
N02W-21-2
8/28/09
125 mL
HDPE
overlying water tank #1
control Nitrite-Day 21
Cold
X
N02W-21-3
8/28/09
125 mL
HDPE
overlying water tank #1
6.25mg/L Nitrite-Day 21
h2so4
> •,<
' T^:-5
x
N02W-21-4
8/28/09
125 mL
HDPE
overlying water tank #1
6.25mg/L Nitrite -Day 21
Cold
X
N02W-21-5
8/28/09
125 mL
HDPE
overlying water tank #1
25 rng/L Nitrite -Day 21
h2so4
X •• •
N02W-21-6
8/28/09
125 mL
HDPE
overlying water tank #1
25 mg/L Nitrite -Day 21
Cold
\ ¦¦¦ ¦ i
X
Shipper:
Date/Time:
Receiver:
Date/Time:
Temperature:
BOD5 = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NO3/NO2-N = Nitrate/Nitrite-Nitrogen
Definitions
TDS - Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NO2-N = Nitrite Nitrogen Total N = Total Nitrogen
NO, -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone; 231-941-2230
Fax: 231-941-2240
www.glee.coro
Great Lakes Environmental Center
Facility:
. .. Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mai lee Garton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description (Site
information / sample type)
Preservation
Check the requested analysis (see definitions below'
¦J*.
¦ < '
«•>
Q
O
ffl
Chloride
Chlorophyll a
Hardness
Z
a
z
z
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
T Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mai lee Carton/Jamie Saxton
m. garlon
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below"
£ :
c <
-
-
<
vs
Q
O
m
Chloride
Chlorophyll a
Hardness
Z
X
z
ss'
o-
z
0
z
Cfl
Q
H
M-r'
H
•ca
on
: Nitrate .
Nitrite
N02W-24-1
8/31/09
125 mL
HDPE
overlying water tank #2
control Nitrite-Day 24
h2so4
X
N02W-24-2
8/31/09
125 mL
HDPE
overlying-water tank ft2
control Nitrite-Day 24
Cold
X
N02W-24-3
8/31/09
125 mL
HDPE
overlying water tank #1
12.5mg/L Nitrite-Day 24
H2SO4
X-'- '
N02W-24-4
8/31/09
125 mL
HDPE
overlying water tank #1
12.5mg/L Nitrite -Day 24
Cold
X
N02W-24-5
8/31/09
125 mL
HDPE
overlying water tank #1
50 mg/L Nitrite -Day 24
HjSO.,
X
N02W-24-6
8/31/09
125 mL
HDPE
overlying water tank #1
50 mg/L Nitrite-Day 24
Cold
X
Shipper:
Date/Time:
Receiver:
Stshj ^
J
Date/Time:
o
Temperature:
BODs = Biochemical Oxygen Dernand-5 day
NH3 -N = Ammonia-Nitrogen
NOj/NOj.-N = N itrate/Nitrite-N itrogen
Definition*
TDS - Total Dissolve Solids TP = Total Phosphorus
TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NO; -N =Nitrite Nitrogen Total N = Total Nitrogen
NO3 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page of
Gs est Ei «ir c-nr:s<>:?Ui)
739 Hastings St.
Traverse City, Ml 49686
Phone:231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
. . Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
)
Alkalinity
ft
o
n
. "E
' £ '
2
y
Chlorophyll a
Hardness
2
S
z
- . "
HDPE
Water 6.25 mg/L
nitrite Tank #2- day
25
Cold
X
NQ2W-25-3
9/1/09
125 mL
HDPE
Overlying water Tank
#2 12.5 mg/L nitrite-
day 25
H2S04
X-
T.4.
'i
N02W-25-4
9/1/09
125 mL
HDPE
Overlying water Tank
#2 12.5 mg/L nitrite-
day 25
Cold
... ..
X
N02W-25-5
9/1/09
125 mL
HDPE
Overlying water Tank
# 2 25mg/L nitrite-
day 25
H2S04
X
N02W-25-6
9/1/09
125 mL
HDPE
Overlying water Tank
#2 25mg/L nitrite-
day 25
Cold
-;/r;
X
Shipper:
Date/Time:
Receiver:
o <4
Date/Time:
I-!-*!
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NH3 -N ~ Ammonia-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP - Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N - Total Nitrogen
NOj -N = Nitrate Nitrogen SRP ** Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Page of
GleC
Giuat Lakes Efws/onmcHrtal Cc-t^r
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
. . Traverse City, MI
Location: ^
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Check the requested analysis (see definitions below'
Bottle ID
Date /Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description (Site
information / sample
type)
Preservation
| Alkalinity
ft
a
o
CD
Chloride
Chlorophyll a
Hardness
Z
i*»
S3
Z
z,
1
*
o
z
o
z
w
a
H
i
Dm
E*
-H
Nitrate
Nitrite
N02W-25-7
9/1/09
125 mL
HDPE
Overlying water Tank # 2
50mg/L nitrite- day 25
H2S04
Ir
,; V..:l
x'
Hi'-;
N02W-25-8
9/1/09
125 mL
HOPE
Overlying water Tank # 2
5Qmg/L nitrite- day 25
Cold
X
N02W-25-9
9/1/09
125 mL
HDPE
Overlying water Tank #2
lOOmg/L nitrite- day 25
H2S04
.
NO2W-25-I0
9/1/09
125 mL
HDPE
Overlying water Tank # 2
lOOmg/L nitrite-day 25
Cold
X
x|fv;
Shipper:
Date/Time:
Receiver:
i)-
Date/Time:
Temperature:
BODs = Biochemical Oxygen Demand-S day
NHj -N = Ammonia-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN - Total Kjeldahl Nitrogen
TP - Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently reoaested analyses
NOi -N - Nitrite Nitrogen Total N - Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of
Date:
Contact Person: Mailee Garton/Jamie Saxton
Cw-l-,
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.g1ee.com
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalinity
(ft
a
0
a
Chloride
Chlorophyll a
Hardness
z
X
z
¦ Z::
r
¦ ¦¦ *%i .
-';Z
• ''in ',s
¦ 9.
to
D
H
fn
a*
w ¦
to. ¦
W '
Nitrate
Nitrite
N02W-26-I
9/2/09
125 mL
HOPE
overlying water tank #3
control Nitrite-Day 26
h2so4
X
N02W-26-2
9/2/09
125 mL
HDPE
overlying water tank #3
control Nitrite-Day 26
Cold
ir v'$1
X
N02W-26-3
9/2/09
125 mL
HDPE
overlying water tank #2
6.25mg/L Nitrite-Day 26
h2so,
X
N02W-26-4
9/2/09
125 mL
HDPE
overlying water tank #2
6.25mg/L Nitrite -Day 26
Cold
X
N02W-26-5
9/2/09
125 mL
HDPE
overlying water tank #2
25 mg/L Nitrite -Day 26
h2so4
X
N02W-26-6
9/2/09
125 mL
HDPE
overlying water tank #2
25 mg/L Nitrite -Day 26
Cold
X
F ... Great Lakes Environmental Center Email Address.
301 ——— Phone Number:
. . Traverse City, MI -
Locat,on: Collector: m* Sarton
Shipper: Date/Time: Receiver: Date/Time: Temperature:
Q , , S /z. JoH U Z-° —Qj_ 9 '"L "^1 —,
2.
Definitions
BODj = Biochemical Oxygen Demand-S day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj -N = Ammonia-Nitrogen TK.N = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NQ3/NO2 -N - Nitratc.'Nitrite-Nitrogcn
Abbreviations for other frequently requested analyses
NOj -N - Nitrite Nitrogen Total N = Total Nitrogen
NO3 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GitC
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Page
of
Mailee Gorton/Jamie Saxton
m. garton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description (Site
information / sample type)
Preservation
Check the requested analysis (see definitions below)
f
w
D
O
m
Chloride
Chlorophyll a
Hardness
T.
rr>
X
z-
z
0
z
• - -M.
"6 •
z
CO
Q
H
0m
H
; th
m
H ¦
U"
• P"l
£
N02W-26-
7
9/2/09
125 ml.
HDPE
overlying water tank #2 100
mg/L Nitrite -Day 26
h2so4
: -v...-
X ¦
N02W-26-
8
9/2/09
125 mL
HOPE
overlying water tank #2 100
mg/L Nitrite -Day 26
Cold
: . ~ :
{ 11
X
X..
N02W-26-
09
9/2/09
125 mL
HDPE
Stock solution 2.1 g/L N02
3.g/L NaN02 - Day 26
H2S04
.-...-I
I - '
:.:V-
/
/
/
N02W-26-
10
9/2/09
125 mL
HDPE
Stock solution 2.1 g/L N02
3.g/L NaN02 - Day 26
Cold
"...
,—.
\
s
' ' ^
Shipper:
2.
Date/Time:
C\!z/W 11-26
Receiver:
BODj = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NO3/HO3 -N = N itrate/N itritc-N itrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP - Total Phosphorus
TSS = Total Suspended Solids
Date/Time:
9-1^
Temperature:
Abbrevistio
i for other I
NOi -N - Nitrite Nitrogen
NO3 -N = Nitrate Nitrogen
Total N = Total Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GlhC
Page.
of
739 Hastings St.
Traverse City, MI 49686
Phone; 231-941-2230
Fax; 231-941-2240
www.alec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Sax ton
m. garton
Bottle IO
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalinity
c
0
K
Chloride
Chlorophyll a
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility;
Page of.
739 Hastings St.
Traverse City, MI 49686
Phone; 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Location:
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below1
Alkalinity
e
o
02
- :
U
Chlorophyll a
Hardness
z
¦
Of
X
z
1
o
z.
o
z
TDS
j TKN
0,
H
«5
CO
H
Nitrite
N02W-32-1
9/8/09
125 mL
HDPE
Water control Tank #1
Cold
X
N02W-32-2
9/8/09
125 mL
HDPE
Water control Tank#2
Cold
X
N02W-32-3
9/8/09
125 mL
HDPE
Water control Tank #3
Cold
X
N02W-32-4
9/8/09
125 mL
HDPE
Water control Tank #4
Cold
X •
N02W-32-5
9/8/09
125 mL
HDPE
Water 6.25 mg/L
nitrite Tank #1
Cold
X
N02W-32-6
9/8/09
125 mL
HDPE
Water 6.25mg/L
nitrite Tank #2
Cold
X
Shipper:
2.
Date/Time:
q/a/frt
Receiver:
Date/Time:
9-H
Temperature:
Definitions
BOD; = Biochemical Oxygen Demand-5 day TDS - Total Dissolve Solids TP = Total Phosphorus
HHj -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NOj/NOj -N = Nitrale/Nitrite-Nitrogen
Abbreviations for other frequently requested analyses
N02 -N - Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page
of
739 Hastings St
Traverse City. MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.elec.coTn
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garten/Jamie Saxton
Check the requested analysis (see definitions below
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
-2?
:
"5'
-
< ¦
t*1
a
0
m
Chloride
Chlorophyll a
Hardness
7.
3?
Z
z,
1
0
z.
<*>
0
*
w
a
H
H
f-
CO
CO
H
Nitrite
N02W-32-7
9/8/09
125 rnL
HDPE
Overlying water Tank
#3 6 25mg/L nitrite
Cold
X
N02W-32-8
9/8/09
125 niL
HOPE
Overlying water Tank
#4 6.25 mg/L nitrite
Cold
.y.
X
N02W-32-9
9/8/09
125 mL
HDPE
Overlying water Tank
#1 12.5 mg/L nitrite
Cold
X
NO2W-32-10
9/8/09
125 mL
HDPE
Overlying water Tank
#2 12.5 mg/L nitrite
Cold
X
N02W-32-11
9/8/09
125 mL
HDPE
Overlying water Tank
#3 12.5mg/L nitrite
Cold
X' -
N02W-32-12
9/8/09
125 mL
HDPE
Overlying water Tank
#4 12.5mg/L nitrite
Cold
X
Shipper:
2.
Date/Time:
^Ib Jo^H
Receiver:
Ar. 0
A
Date/Time:
9-M
Temperature:
Definitions
BODs = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj -N = Ammonia-Nitrogen TKN - Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NOj/NOi -N = Nitrate/Nitrite-Nitrogen
Abbreviations for other freouentlv requested analyses
NO*-N "Nitrite Nitrogen Total N = Total Nitrogen
NO3 -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Page of
739 Hastings St
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
. . Traverse City, Ml
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
Alkalinity
VJ
o
o
pa
Chloride
Chlorophyll a
Hardness
7.
5
Z.
z
o
•Z'
o
z
tfl
Q
i-
H
0.
H
m
w
H
Nitrite
N02W-32-13
9/8/09
125 mL
HDPE
Overlying water Tank
#1 25mg/L nitrite
Cold
X
N02W-32-14
9/8/09
125 mL
HDPE
Overlying water Tank
#2 25mg/L nitrite
Cold
X
N02W-32-15
9/8/09
125 mL
HDPE
Overlying water Tank
#3 25mg/L nitrite
Cold
X
N02W-32-16
9/8/09
125 mL
HDPE
Overlying water Tank
#4 25mg/L nitrite
Cold
X
N02W-32-17
9/8/09
125 mL
HDPE
Overlying water Tank
#1 50mg/L nitrite
Cold
X
N02W-32-18
9/8/09
125 mL
HDPE
Overlying water Tank
#2 50mg/L nitrite
Cold
X
Shipper:
1. Cv- .
2.
Date/Time:
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Page of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
T . Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garten/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
o
0Q
Chloride
Chlorophyll a
.un
,V:
¦ ;ff-
T3 ¦
2
£
z
z
rt
O
' z
¦ o
zr.
TDS
H
Bm
w
w
H
Nitrite
N02W-32-19
9/8/09
125 mL
HOPE
Overlying water Tank
#3 50mg/L nitrite
Cold
X
NO2W-32-20
9/8/09
125 mL
HDPE
Overlying water Tank
#4 50mg/L nitrite
Cold
X
N#2W"32"2'
9/8/09
125 mL
HOPE
Overlying water Tank
#1 lOOmg/L nitrite
Cold
X
N02W-32-22
9/8/09
125 mL
HDPE
Overlying water Tank
#2 lOOmg/L nitrite
Cold
X
9/8/09
125 mL
HDPE
Overlying water Tank
#3 lOOmg/L nitrite
Cold
X
[9/8/09
125 mL
HDPE
Overlying water Tank
#4 lOOmg/L nitrite
Cold
X
Shipper:
Ck -!>•» k> CfljJU/ QL.
Date/Time:
Receiver:
2.
Date/Time:
Q-t -1
Temperature:
BOD5 = Biochemical Oxygen Demand-5 day
NH3 -K = Ammonia-Nitrogen
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldaht Nitrogen
TP - Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N - Total Nitrogen
NOi -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page of*
739 Hastings St.
Traverse City. MI 49686
Phone: 231-941-2230
Fax:231-941-2240
www.giec.com
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
£•1
O
oa
•
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of
739 Hastings St.
Traverse City. MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
wvAV.glec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Cheek the requested analysis (see definitions below
)
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
¦ ¦¦
"at
<
O
O
pa
Chloride
Chlorophyll a
«l
s '
W - :
SB
SS
z
i"5
s
z
z
0
%
0 ¦
z
a
TKN
cu
H
STj
m
H
Nitrite
Nitrate
N02W-32-3 1
9/8/09
125 nil.
HDPE
Overlying water Tank
#3 6.25mg/L nitrite
h2so4
X
N02W-32-32
9/8/09
125 raL
HDPE
Overlying water Tank
#4 6.25 mg/L nitrite
h2so4
X
N02W-32-33
9/8/09
125 ml..
HDPE
Overlying water Tank
#1 12.5 mg/L nitrite
h2so4
X
N02W-32-34
9/8/09
125 ml.
HDPE
Overlying water Tank
#2 12.5 mg/L nitrite
H2S04
X
N02W-32-35
9/8/09
125 mL
HDPE
Overlying water Tank
#3 12.5mg/L nitrite
H2S04
X
N02W-32-36
9/8/09
125 mL
HDPE
Overlying water Tank
#4 12.5mg/L nitrite
h2so4
X
Shipper:
2.
Date/Time:
I<*10*1
Receiver:
Date/Time:
Temperature:
BOD, = Biochemical Oxygen Demand-5 day
NHS -N = Ammonia-Nitrogen
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TK.N = Total Kjeldaht Nitrogen
TP - Total Phosphorus
TSS - Total Suspended Solids
Abbreviations for other frequently requested analyses
NOi -N = Nitrite Nitrogen Total N = Total "Nitrogen
N03 -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Facility:
Location:
Page of
739 Hastings St,
Traverse City, MI 49686
Phone: 23 J-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/.lanlie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
«rs
a
0
sa
Chloride
Chlorophyll a
Vi
C. " •
T5 '
-im
CC
sa.
z
1
X
z
z
0
z
n
0
z-
00
a
TKN
Ok
H
GO
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
,V.
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Page of
Great Lakes Environmental Center
Facility:
. .. Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysis (see definitions below;
1
Alkalinity
BOD,
Chloride
Chlorophyll a
Hardness
Z
rO
ffi
Z
z
r*
O
Z
m
O
z
«a
a
TKN
&
w
w
H
Nitrite
Nitrate
N02W-32-43
9/8/09
125 mL
HOPE
Overlying water Tank
#3 50mg/L nitrite
h2so4
X
N02W-32-44
9/8/09
125 mL
1-IDPE
Overlying water Tank
#4 5Qmg/L nitrite
HUSO,.
X
9/8/09
125 mL
HDPE
Overlying water Tank
#1 lOOmg/L nitrite
H?SO,
X
N02W-32-46
9/8/09
125 mL
HDPE
Overlying water Tank
#2 lOOmg/L nitrite
H2SO.,
X
MQ2W-33-4?-
9/8/09
125 mL
HDPE
Overlying water Tank
#3 lOOmg/L nitrite
h2so4
X
[N02W-32- 48
9/8/09
125 mL
HDPE
Overlying water Tank
#4 lOOmg/L nitrite
h2so4
X
Shipper: Date/Time: Receiver: Date/Time: Temperature:
l Q- /ft jo ^ fl\^„ /Q (A
2.
Definitions
BODj - Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NH3 -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Abbreviations for other frequently requested analyses
NO2 -N = Nitrite Nitrogen Total N = Total 'Nitrogen
N03 -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Gu:C
Page of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230'
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
, . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Check the requested analysis (see definitions below)
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description (Site
information / sample type)
Preservation
Alkalinity
VI
0
O
03
Chloride
Chlorophyll a
Hardness
Z
sc
z
z
c
z
£
z
TDS
TKN
0*
GO
H
Nitrite
Nitrate
N02W-32-49
9/8/09
125 mL
HOPE
Stock solution Stock solution
Nitrite 2.1 g/LN02
3.2g/L NaN02
h2so4
X
NO2W-32-50
9/8/09
125 mL
HDPB
Stock solution Stock solution
Nitrite 2.1 g/L N02
3.2g/L NaN02
Cold
X
X
X
X
X
Shipper:
Date/Time:
Receiver:
j)/!
2.
Date/Time:
Temperature:
BOD5 = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NOj/NOj -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN - Total Kjcldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NO2 -N = Nitrite Nitrogen Total N = Total Nitrogen
NO5 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
GleC
Cimt Lakes BTvironmsniaS Cento?-
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
TEST MATERIAL:
PROJECT NUMBER:,
Nitrite. TYPE OF TEST: DILUTION WATER:. \VCKIor
fc 113-13- NUMBER OF FISH/CHAMBER: D1LUTOR NUMBER:_ FHOTOFERIOP: ) (/ •
TEST SPECIES: FHIV\ NUMBER OF CHAMBERS: ^ TEST TEMPERATURE: 2-5- t ° C flQE QF nSH.
DATE
TEST
TIME
(DAYS)
TECH.
INIT.
TREATMENT
Li
diktyp!
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1^. 6 r*Y\
TIME
REPLICATE NUMBER
1
2
3
4
1
2
3
4
t
2
3
4
u\
0
Temperature
M.U>
55. 1
9S3
^5-6
3^
3S1
pH
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WH
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Alkalinity
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Ammonia as N
1
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Js.fe?
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35-1
2
Temperature
asn
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3
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25
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3eS
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BPS'
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330
Hardness
J
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Alkalinity
/
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24^
2-4^
2H?i
:2_4S
-------
jfLEC
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
~5 ., "'~C.iT/itGrinienia! Cflr.tcf
TEST MATERIAL:
PROJECT NUMBER:.
TEST SPECIES;
TYPE OF TEST:_
NUMBER OF FISH/CHAMBER;
NUMBER OF CHAMBERS:.
DILUTION WATER:
DILUTOR NUMBER:
PHGTOPERIOD:.
fci
TEST TEMPERATURE:
.AGE OF FISH:.
DATE
TEST
TIME
TECH.
1NIT.
TREATMENT
f'/jO - Jklofc.
(h-2S OWiu
! ">
l0
TIME
REPLICATE NUMBER
1
2
3
4
1
2
3
4
1
2
3
A
O
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f¥0
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3ft. 5
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m
71
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Observation Key;
-------
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
TEST MATERIAL:_ TYPE OF TEST: , DILUTION WATER:_
PROJECT NUMBER: NUMBER OF FISH/CHAMBER: DILUTOR NUMBER. _ PHOTOPERIOD:
TEST SPECIES; Firm NUMBER OF CHAMBERS: L| TEST TEMPERATURE: <^S"I" AGE OF FISH:
DATE
TEST
TIME
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INIT.
TREATMENT
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1
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FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
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FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
TEST MATERIAL: N l*Ti aXA TYPE OF TEST: DILUTION WATER: Qg
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TEST MATERIAL:
FATHEAD MINNOW EARLY LIFE-STAGE FLOW-THROUGH TOXICITY TEST
TYRE OF TEST:
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GleC
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6
-------
PROJECT#:
PROJECT NAME
CALIBRATION
6112-12
Nitrate Nitrite Tox Testing
Date
Test Day
Test Type
07/08/2009
0
Sediment Tox
Concentrations (
ppm)
Natural log
Millivolts
0.5
-0,6931472
-48.3
1
0
-61.4
10
2.30258509
-118,6
25
3.21887582
-141.0
so
3.91202301
-158.0
Regression Output:
Constant
-63.315773
Std Err of Y Est
1.498825
R Squared
0.999283
No. of Observations
5
Degrees of Freedom
3
X Coefficient(s)
Std Err of Coef.
-24.109041
0.37274591
OUTPUT
Predicted
Ammonia
Test
GLC
Sample
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
ID
Day
volts
Log
Levels
Blank
32 day FHM w/NQ2
Con
Lab control
0
3.6
-2.775547
0.06231539
-0.1877515
32 day FHM w/N02
6.2 5 me/1
3.5
-2.771399
0.0625744
-0.1874925
32 day FHM w/N02
12.5mg./l
17.6
.356242
0,03486604
-0.2152009
32 day FHM w/N02
25mg/l
17.1
-3.335503
0.03559668
-0.2144703
32 day FHM w/N02
50mg/l
16.5
-3.310616
0,03649369
-0.2135732
32 day FHM w/N02
100 mg/l
17.9
-3.368685
0.03443488
-0.2156321
n/a
Blank
1
0 i
-29.9
-1,386027 |
0.25006693
0
-------
PROJECT#; 6112-12 & 2017-00 Date 10/08/2009
PROJECT NAME Nitrate Nitrite Tox Testing
Boyne Test Day 3
CALIBRATION
Concentrations i
ppm)
Natural log
Millivolts
0.5
-0.6931472
-46,5
1
0
-60.7
10
2.30258509
-117.7
25
3.21887582
-140.3
50
3.91202301
-158.2
Regression Output:
Constant
-62.046882
Std Err of Y Est
1.228677
R Squared
0.999529
No. of Observations
5
Degrees of Freedom
3
X CoefFicient(s) -24.388716
Std ErrofCoef. 0,30556212
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
Nitrite Tank 1
3
FHM
eon
3 1
water
22.6
-3.470740 |
0.03109402
-0.2075191
FHM
1 6.25
3 1
water
25
-3.569146 I
0.02817991
-0.2104332
FHM
I 12,5
*
water
24.7
-3.556845
0.02852869
-0.2100844
FHM
|
water
22.9
-3.483040 |
0.03071389
-0.2078992 I
FHM
1 50
3 1
water
23
-3.487141 |
0.03058821
-0.2080249
FHM
100
3
water
24.4
-3.544544
0.02888178
-0.2097313 1
: Nitrite Tank 4
|
1
|
FHM
I con
3 1
water
27.3
-3.663452 |
0.02564384
-0.2129692 I
FHM
6.25 |
3 1
water |
16,6
-3.224724 I
0.03976675
-0.1988463 |
FHM
12.5
3 1
water
21.7
-3.433837 |
0.0322629
-0.2063502 |
FHM
25
3
water
22.9 !
-3.483040 |
0.03071389
-0.2078992 1
FHM
50
? !
water
23.4 |
-3.503542 |
0.03009062
-0.2085225 I
FHM !
100 1
3 1
water 1
23.9 1
-3.524043 |
0.02948001
-0.2091331 |
|
||
|
|
| |
I
n/a
Blank 1
3 1
n/a
-27.1
-1.432912 1
0.23861308
0
-------
PROJECT#: 6112-12 Date 17/08/2009
PROJECT NAME Nitrate Nitrite Tox Testing
Test Day Nitrite: 10
CALIBRATION
Concentrations i
ppm)
Natural log
Millivolts
0.5
-0.6931472
-51.4
1
0
-61.6
10
2.30258509
-117.0
25
3.21887582
-139.6
50
3.91202301
-157.5
Regression Output:
Constant
-64.567273
Std Err of Y Est
2.720863
R Squared
0.997491
No. of Observations
5
Degrees of Freedom
3
X Coefficients) -23.370225
Std Err of Coef. 0.67665698
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
Nitrate Repl
Nitrite Rep 1
eon
na
10
water
28.1
-3.965185
0.01896452
-0.1572853
6.25
na
10
water
32.4
-4.149180
0.01577735
-0.1604725
12.5
na
10
water
29.7
-4.033648
0.0177096
-0.1585402
25
na
10
water
33.6
-4.200527
0.01498767
-0.1612621
50
na
10
water
32.7
-4.162017
0.01557611
-0.1606737
100
na
10
water
31.3
-4.102112
0.01653772
-0.1597121
Nitrite Rep 4
con
na
10
water
32.5
-4,153459
0.01570998
-0.1605398
6.25
na
10
water
32.5
-4.153459
0.01570998
-0.1605398
12.5
na
10
water
33.2
-4.183412
0.0152464
-0.1610034
25
na
10
water
33.3
-4,187691
0.0151813
-0.1610685
50
na
10
water
33.7
-4.204806
0,01492367
-0.1613261
100
na
10
water
34.7
-4.247596
0.01429857
-0,1619512
n/a
Blank
Nitrite: 10
n/a
-24
-1.735853
0.17624982
0
-------
PROJECT#: 6112-12 Date 25/08/2009
PROJECT NAME Nitrate Nitrite Tox Testing
Test Day 18
CALIBRATION
Concentrations
ppm)
Natural log
Millivolts
0.5
-0.6931472
-26.6
1
0
-38.4
10
2.30258509
-95.7
25
3.21887582
-118,9
50
3.91202301
-136.8
Regression Output:
Constant
-40.908656
Std Err of Y Est
2.212824
R Squared
0.998456
No. of Observations
5
Degrees of Freedom
3
X Coefficient(s) -24,238966
Std Err of Coef. 0.55031181
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
Nitrate Repl
Nitrite Rep 2
con
na
18
water
-4
-1.522699
0.21812231
-0.009187
6.25
na
18
water
1
-1,728979
0.17746556
-0.0498438
12.5
na
18
water
2
-1,770235
0.17029303
-0.0570163
25
na
18
water
1.5
-1,749607
0.17384231
-0.053467
50
na
18
water
1
-1.728979
0.17746556
-0.0498438
100
na
18
water
2.4
-1.786737
0.16750586
-0.0598035
Nitrite Rep 3
eon
na
18
water
-1
-1,646467
0.19272963
-0.0345797
6,25
na
18
water
1.5
-1.749607
0.17384231
-0.053467
i 2.5
na
18
water
2.5
-1.790863
0.16681622
-0,0604931
25
na
18
water
1
-1.728979
0.17746556
-0,0498438
50
na
18
water
2.3
-1.782611
0.16819834
-0.059111
100
na
18
water
2.7
-1.799114
0.16544545
-0.0618639
n/a
Blank
18
n/a
-5
-1.481443
0.22730935
0
-------
PROJECT#: 6112-12 Date 31/08/2009
PROJECT NAME HECD WA B12-Nitratc; Nitrite Tox Testing
Test Day 20
CALIBRATION Test Type Sediment Tox
Concentrations i
[ppm)
Natural log
Millivolts
0,5
-0,6931472
-24.2
1
0
-35.9
10
2.30258509
-94.1
25
3.21887582
-117.8
50
3.91202301
-131.7
Regression Output:
Constant
-38.726752
Std Err of Y Est
2.349771
R Squared
0.998230
No. of Observations
5
Degrees of Freedom
3
X Coefficient(s) -24.034113
Std Err of Coef. 0.58436929
OUTPUT
Test
Organism
GLC
Number
Test
Day
Milli-
volts
Natural
Log
Predicted
Ammonia
Levels
Ammonia
Minus
Blank
Sample
ID
FHM N02-32 day
Con
repl
below blank
-1.611324
0.19962306
-0.1055343
FHM N02-32 day
Con
rep4
below blank
-1.611324
0.19962306
-0.1055343
FHM N02-32 day
6.25
repl
below blank
-1.611324
0.19962306
-0.1055343
FHM N02-32 day
6.25
rep4
below blank
-1.611324
0.19962306
-0.1055343
FHM N02-32 day
12.5
repl
below blank
-1.611324
0.19962306
-0.1055343
FHM N02-32 day
12.5
rep4
below blank
-1.611324
0.19962306
-0.1055343
FHM N02-32 day
25
repl
below blank
-1.611324
0.19962306
-0.1055343
FHM N02-32 day
25
rep4
below blank
-1.611324
0.19962306
-0.1055343
FHM N02-32 day
50
repl
below blank
-1.611324
0.19962306
-0.1055343
FHM N02-32 day
50
rep4
below blank
-1.611324
0.19962306
-0.1055343
FHM N02-32 day
100
repl
below blank
-1.611324
0.19962306
-0.1055343
FHM N02-32 day
100
rep4
below blank
-1.611324
0.19962306
-0.1055343
n/a |
Blank 1
1
20 1
-10.2 |
-1.186928
0.30515739 1
0
-------
PROJECT#; 6112-12 Date 08/09/2009
PROJECT NAME HECD WA B12-Nitrate, Nitrite Tox Testing
Test Day 32
CALIBRATION Test Type Sediment Tox
Concentrations i
jppm)
Natural log
Millivolts
0.5
-0.6931472
-25.1
1
0
-36.1
10
2.30258509
-93.6
25
3.21887582
-117.3
50
3.91202301
-134.7
Regression Output:
X Coeffieient(s) -24,215481
Std Err of Coef. 0.62803912
OUTPUT
Test
Organism
GLC
Number
Test
Day
Milli-
volts
Natural
Log
Predicted
Ammonia
Levels
Ammonia
Minus
Blank
Sample
ID
FHM N02-32 day
Con
repl
33.9
-3.011698
0,04920807
0.01527485
FHM N02-32 day
Con
rep4
21.9
-2.516147
0.08077022
0.046837
FHM N02-32 day
6.25
repl
46.7
-3.540285
0.02900506
-0,0049282
FHM N02-32 day
6.25
rep4
40.7
-3.292510
0.03716047
0.00322725
FHM N02-32 day
12.5
repl
44.5
-3.449434
0.0317636
-0.0021696
FHM N02-32 day
12.5
rep4
47.2
-3.560933
0.0284123
-0.0055209
FHM N02-32 day
25
repl
42
-3.346194
0.03521813
0.0012849
FHM N02-32 day
25
rep4
34.9
-3.052994
0.04721737
0.01328414
FHM N02-32 day
50
repl
39.5
-3.242955
0.03904835
0.00511513
FHM N02-32 day
50
rep4
47.7
-3.581581
0.02783166
-0.0061016
FHM N02-32 day
100
repl
55
-3.883041
0.02058812
-0.0133451
FHM N02-32 day
100
rep4
48
-3.593970
0.02748899
-0.0064442
n/a
Blank
32 I
42.9 1
-3.383361 |
0,03393322
0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
Dilution Water ControlRep 1
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
294.0 136.0 112.0 ND
298,0 ND
07/08/2009
0
24.6
7.82
7.6
08/08/2009
1
09/08/2009
2
25.4
10/08/2009
3
24.9
8.26
8.2
11/08/2009
4
12/08/2009
5
25.0
8.34
8.0
13/08/2009
6
24.9
14/08/2009
7
15/08/2009
8
24.4
16/08/2009
9
17/08/2009
10
24.6
8.04
8.0
18/08/2009
11
19/08/2009
12
20/08/2009
13
24.9
21/08/2009
14
24.5
8.24
7,6
22/08/2009
15
23/08/2009
16
25.0
24/08/2009
17
25/08/2009
18
26/08/2009
19
24.3
7.75
8.0
27/08/2009
20
28/08/2009
21
24.5
7.75
7.4
29/08/2009
22
30/08/2009
23
24.9
31/08/2009
24
24.9
8.00
6.9
01/09/2009
25
02/09/2009
26
24.0
8.02
7.1
03/09/2009
27
04/09/2009
28
25.0
8.02
8.5
05/09/2009
29
25.0
06/09/2009
30
07/09/2009
31
08/09/2009
32
24.8
8.08
6.9
ND
303.0
294.0 ND
301.0 100.0 98.0 ND
N
18
11
11
5
2
2
Max
25.4
8.34
8.5
303.0
136.0
112,0
Min
24.0
7.75
6.9
294.0
100.0
98.0
Mean
24.8
8.03
7.7
298.0
118,0
105.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
Dilution Water ControlRep 2
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
07/08/2009
0
24,8
8.21
7.9
08/08/2009
1
25.3
09/08/2009
2
10/08/2009
3
11/08/2009
4
26.3
12/08/2009
5
25,4
8.35
8.0
13/08/2009
6
14/08/2009
7
25.3
8,07
9.1
15/08/2009
8
24.6
16/08/2009
9
17/08/2009
10
24,7
8.03
7.7
18/08/2009
11
19/08/2009
12
25.1
8.41
8.1
20/08/2009
13
21/08/2009
14
25.7
8.26
8.0
22/08/2009
15
23/08/2009
16
26.1
24/08/2009
17
25.1
7.70
7,8
25/08/2009
18
26/08/2009
19
27/08/2009
20
25,2
28/08/2009
21
29/08/2009
22
25.0
30/08/2009
23
24.3
31/08/2009
24
01/09/2009
25
02/09/2009
26
03/09/2009
27
24.9
04/09/2009
28
25.5
8.04
8.3
05/09/2009
29
06/09/2009
30
07/09/2009
31
25.6
08/09/2009
32
N 17
Max 26.3
Min 24.3
Mean 25.2
8
8
1
8.41
9.1
304,0
7.70
7.7
304.0
8.13
8.1
304.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
Dilution Water ControlRep 3
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
07/08/2009
0
24.4
8.13
7.8
08/08/2009
1
25.3
09/08/2009
2
25.7
10/08/2009
3
25.1
11/08/2009
4
12/08/2009
5
25.4
13/08/2009
6
24.9
14/08/2009
7
25.3
8.21
9.4
15/08/2009
8
16/08/2009
9
25,5
17/08/2009
10
25,2
18/08/2009
11
25.2
19/08/2009
12
20/08/2009
13
25.3
21/08/2009
14
22/08/2009
15
24,7
23/08/2009
16
24/08/2009
17
25.2
8.22
7,9
25/08/2009
18
25.5
26/08/2009
19
25,6
8.20
7.9
27/08/2009
20
28/08/2009
21
25,3
8.07
7.8
29/08/2009
22
30/08/2009
23
31/08/2009
24
01/09/2009
25
25.4
02/09/2009
26
25.0
8.19
7.4
03/09/2009
27
24.8
04/09/2009
28
05/09/2009
29
06/09/2009
30
07/09/2009
31
25.6
08/09/2009
32
N
20
6
6
Max
25,7
8.22
9.4
Min
24.4
8,07
7.4
Mean
25.2
8.17
8.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas {Fathead Minnow)
Dilution Water Control Rep 4
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
8.10 7,9 ND
8.33 8.2 305,0 ND
07/08/2009
0
25.7
08/08/2009
1
09/08/2009
2
25,7
10/08/2009
3
25.4
11/08/2009
4
26.2
12/08/2009
5
24.8
13/08/2009
6
14/08/2009
7
15/08/2009
8
16/08/2009
9
25.3
17/08/2009
10
25.2
18/08/2009
11
25.8
19/08/2009
12
25.8
20/08/2009
13
21/08/2009
14
22/08/2009
15
24.7
23/08/2009
16
24/08/2009
17
25/08/2009
18
25.2
26/08/2009
19
27/08/2009
20
25.2
28/08/2009
21
29/08/2009
22
24.9
30/08/2009
23
31/08/2009
24
24.2
01/09/2009
25
25.1
02/09/2009
26
03/09/2009
27
04/09/2009
28
05/09/2009
29
25.3
06/09/2009
30
07/09/2009
31
08/09/2009
32
24.9
ND
7.86 8.1
7.84 5.8 292.0 ND
8.01 6.3 305.0 132.0 102,0 ND
N
17
5
5
3
1
1
Max
26.2
8.33
8.2
305.0
132.0
102.0
Min
24.2
7.84
5.8
292.0
132.0
102.0
Mean
25.3
8,03
7.3
300.7
132.0
102.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
6.25 mg/L Nitrate Rep 1
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
312.0 156.0 104.0 ND
318.0 ND
07/08/2009
0
24.6
8.19
8.2
08/08/2009
1
09/08/2009
2
25.4
10/08/2009
3
24.7
8.32
8.2
11/08/2009
4
12/08/2009
5
25.0
8.36
8.0
13/08/2009
6
24.9
14/08/2009
7
25.4
8.25
9.1
15/08/2009
8
25.5
16/08/2009
9
17/08/2009
10
25.2
8.18
7.7
18/08/2009
11
19/08/2009
12
20/08/2009
13
25.1
21/08/2009
14
25.0
8.23
7.7
22/08/2009
15
23/08/2009
16
25.2
24/08/2009
17
25/08/2009
18
26/08/2009
19
24.5
8.14
8.0
27/08/2009
20
28/08/2009
21
24.5
8.14
7.7
29/08/2009
22
30/08/2009
23
24.8
31/08/2009
24
24.1
8.02
6.7
01/09/2009
25
02/09/2009
26
24.8
8,19
7.6
03/09/2009
27
04/09/2009
28
25.3
8.05
8.0
05/09/2009
29
06/09/2009
30
07/09/2009
31
08/09/2009
32
24.4
8,13
7.0
ND
325.0
297.0 ND
322.0 136.0 102.0 ND
N
18
12
12
5
2
2
Max
25.5
8.36
9.1
325.0
156.0
104.0
Min
24.1
8.02
6.7
297.0
136.0
102.0
Mean
24.9
8.18
7.8
314.8
146.0
103.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
6,25 mg/L Nitrate Rep 2
Date Test Day Temperature pH
07/08/2009
0
25.1
8.04
08/08/2009
1
25.4
09/08/2009
2
10/08/2009
3
11/08/2009
4
26.0
12/08/2009
5
25.2
8.40
13/08/2009
6
14/08/2009
7
15/08/2009
8
24.5
16/08/2009
9
17/08/2009
10
252
8.18
18/08/2009
11
19/08/2009
12
25.7
8,56
20/08/2009
13
21/08/2009
14
25.5
8.27
22/08/2009
15
23/08/2009
16
26.0
24/08/2009
17
25.0
8.24
25/08/2009
18
26/08/2009
19
27/08/2009
20
25,4
28/08/2009
21
29/08/2009
22
25,1
30/08/2009
23
24.0
31/08/2009
24
01/09/2009
25
02/09/2009
26
03/09/2009
27
24.6
04/09/2009
28
25.5
8.09
05/09/2009
29
06/09/2009
30
07/09/2009
31
25.5
08/09/2009
32
SC Hardness Alkallinity Ammonia
8.0
7.9
7.7 324.0
8.3
78
7.8
ND
N 16 7 7 1
Max 26.0 8.56 8,3 324.0
Min 24.0 8.04 7.7 324,0
Mean 25.2 8.25 8.0 324.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
6.25 mg/L Nitrate Rep 3
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
8.09 7.8
07/08/2009
0
25.3
08/08/2009
1
09/08/2009
2
10/08/2009
3
25.4
11/08/2009
4
12/08/2009
5
25.2
13/08/2009
6
14/08/2009
7
15/08/2009
8
16/08/2009
9
25.2
17/08/2009
10
25.1
18/08/2009
11
25.8
19/08/2009
12
20/08/2009
13
25.0
21/08/2009
14
22/08/2009
15
24.5
23/08/2009
16
24/08/2009
17
24.9
25/08/2009
18
25.1
26/08/2009
19
25.1
27/08/2009
20
28/08/2009
21
24.8
29/08/2009
22
30/08/2009
23
31/08/2009
24
01/09/2009
25
25.0
02/09/2009
26
24.9
03/09/2009
27
24.5
04/09/2009
28
05/09/2009
29
06/09/2009
30
07/09/2009
31
25,5
08/09/2009
32
8.25 8.0
ND
8.18 7,7
8.17 7.9
8.15 7.9
N
16
5
5
Max
25.8
8.25
8.0
Min
24.5
8,09
7.7
Mean
25.1
8.17
7.9
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
6.25 mg/L Nitrate Rep 4
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
07/08/2009 0 25.6 8.21 7.8 ND
08/08/2009 1
09/08/2009 2 25.4
10/08/2009 3 25.4 8.36 8.0 325.0 ND
11/08/2009 4 26.0
12/08/2009 5 25.2
13/08/2009 6 24.9
14/08/2009 7 25.3 8.34 9.1
15/08/2009 8
16/08/2009 9 25.2 ND
17/08/2009 10 25.1
18/08/2009 11 25,7
19/08/2009 12 25.9 8.35 8.4
20/08/2009 13
21/08/2009 14
22/08/2009 15 24.5
23/08/2009 16
24/08/2009 17
25/08/2009 18 25.0
26/08/2009 19
27/08/2009 20 25.3
28/08/2009 21
29/08/2009 22 24.7
30/08/2009 23
31/08/2009 24 24,3 8.00 6.7 305.0 ND
01/09/2009 25 25.0
02/09/2009 26
03/09/2009 27
04/09/2009 28
05/09/2009 29
06/09/2009 30
07/09/2009 31
08/09/2009 32 24.1 8.12 7.0 323.0 132.0 106.0 ND
N
18
6
6
3
1
1
Max
26.0
8.36
9.1
325.0
132.0
106.0
Min
24.1
8.00
6.7
305.0
132.0
106.0
Mean
25.1
8.23
7.8
317.7
132.0
106.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
12.5 mg/L Nitrate Rep 1
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
07/08/2009 0 24.5 8.26 8.2 325.0 148.0 108.0 ND
08/08/2009 1 24.7
09/08/2009 2 25,5
10/08/2009 3 24.9 8.34 8.0 330.0 ND
11/08/2009 4
12/08/2009 5 25.0 8.40 8.0
13/08/2009 6 24,9
14/08/2009 7 25.0 8.29 9.2
15/08/2009 8 25.0
16/08/2009 9 ND
17/08/2009 10 24,8 8.19 7.7 336.0
18/08/2009 11
19/08/2009 12
20/08/2009 13 25,1
21/08/2009 14 25.1 8.25 7.7
22/08/2009 15
23/08/2009 16 25.6
24/08/2009 17
25/08/2009 18
26/08/2009 19 24.6 8,21 8.1
27/08/2009 20
28/08/2009 21 24.7 8.24 7.9
29/08/2009 22
30/08/2009 23 24.9
31/08/2009 24 24.2 8 06 7.0 317.0 ND
01/09/2009 25
02/09/2009 26 24.0 8.16 8.0
03/09/2009 27
04/09/2009 28 25.3 8.11 8,0
05/09/2009 29
06/09/2009 30
07/09/2009 31
08/09/2009 32 24.5 8.14 7.1 330.0 132,0 102.0 ND
N
19
12
12
5
2
2
Max
25.6
8.40
9.2
336.0
148,0
108.0
Min
24.0
8.06
7.0
317.0
132.0
102,0
Mean
24.9
8.22
7.9
327.6
140.0
105.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
12.5 mg/L Nitrate Rep 2
Date Test Day Temperature pH
07/08/2009
0
25.7
8.10
08/08/2009
1
25.1
09/08/2009
2
10/08/2009
3
11/08/2009
4
26.1
12/08/2009
5
24,7
8.35
13/08/2009
6
24.5
14/08/2009
7
25.2
8.31
15/08/2009
8
24.7
16/08/2009
9
17/08/2009
10
24.8
8.17
18/08/2009
11
19/08/2009
12
25.1
8.48
20/08/2009
13
21/08/2009
14
25.1
8.27
22/08/2009
15
23/08/2009
16
25.0
24/08/2009
17
24.3
8,29
25/08/2009
18
26/08/2009
19
27/08/2009
20
25.1
28/08/2009
21
29/08/2009
22
24.1
30/08/2009
23
24,6
31/08/2009
24
01/09/2009
25
02/09/2009
26
03/09/2009
27
24.9
04/09/2009
28
25.0
8.15
05/09/2009
29
06/09/2009
30
07/09/2009
31
25.1
08/09/2009
32
SC Hardness Alkallinity Ammonia
8.0
8.0 245.0
9,2
7.7 338,0
8,2
8,0
8.0
ND
N 18
Max 26.1
Min 24.1
Mean 25,0
8
8
2
8.48
9,2
338.0
8.10
7.7
245,0
8.27
8.1
291,5
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promeias (Fathead Minnow)
Date
12.5 mg/L Nitrate Rep 3
Test Day Temperature pH
07/08/2009
0
24,8
08/08/2009
1
09/08/2009
2
25.6
10/08/2009
3
24.7
11/08/2009
4
12/08/2009
5
25.2
13/08/2009
6
14/08/2009
7
15/08/2009
8
16/08/2009
9
25.1
17/08/2009
10
25.2
18/08/2009
11
25.9
19/08/2009
12
20/08/2009
13
25.6
21/08/2009
14
22/08/2009
15
24.6
23/08/2009
16
24/08/2009
17
25,0
25/08/2009
18
25.0
26/08/2009
19
25.3
27/08/2009
20
28/08/2009
21
25.0
29/08/2009
22
30/08/2009
23
31/08/2009
24
01/09/2009
25
25.0
02/09/2009
26
24.7
03/09/2009
27
24.5
04/09/2009
28
05/09/2009
29
06/09/2009
30
07/09/2009
31
25.2
08/09/2009
32
DO
SC
Hardness Alkallinity Ammonia
8.00
8.1
8.19
8.11
8.14
7.7
7.6
7.5
ND
8.07
8.0
N
17
5
5
Max
25.9
8.19
8.1
Min
24.5
8.00
7.5
Mean
25.1
8.10
7,8
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
12.5 mg/L Nitrate Rep 4
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
8.01 8.0 ND
8.28 8.0 330.0 ND
07/08/2009
0
25.3
08/08/2009
1
09/08/2009
2
25.7
10/08/2009
3
24.7
11/08/2009
4
25.5
12/08/2009
5
25.1
13/08/2009
6
14/08/2009
7
15/08/2009
8
16/08/2009
9
25.1
17/08/2009
10
25.2
18/08/2009
11
25.8
19/08/2009
12
25.9
20/08/2009
13
21/08/2009
14
22/08/2009
15
24.5
23/08/2009
16
24/08/2009
17
25/08/2009
18
25.0
26/08/2009
19
27/08/2009
20
25.0
28/08/2009
21
29/08/2009
22
24.8
30/08/2009
23
31/08/2009
24
24.4
01/09/2009
25
25.1
02/09/2009
26
03/09/2009
27
04/09/2009
28
05/09/2009
29
06/09/2009
30
07/09/2009
31
08/09/2009
32
24.2
ND
8.44 8.2
8.03 6.9 314.0 ND
8,14 7,2 333.0 132.0 104.0 ND
N
16
5
5
3
1
1
Max
25.9
8.44
8.2
333.0
132.0
104.0
Min
24.2
8.01
6.9
314.0
132.0
104.0
Mean
25.1
8.18
7.7
325.7
132.0
104.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
25.0 mg/L Nitrate Rep
Date Test Day Temperature pH D
07/08/2009
0
25.2
8,26
08/08/2009
1
25.1
09/08/2009
2
25.7
10/08/2009
3
25.1
8.27
11/08/2009
4
12/08/2009
5
25.2
8.27
13/08/2009
6
14/08/2009
7
15/08/2009
8
25.6
16/08/2009
9
17/08/2009
10
25.4
8.19
18/08/2009
11
19/08/2009
12
20/08/2009
13
25,1
21/08/2009
14
25,4
8,24
22/08/2009
15
23/08/2009
16
25.8
24/08/2009
17
25/08/2009
18
26/08/2009
19
25.4
8.19
27/08/2009
20
28/08/2009
21
24,7
8,19
29/08/2009
22
30/08/2009
23
24.1
31/08/2009
24
24.4
8.05
01/09/2009
25
02/09/2009
26
24.9
8.09
03/09/2009
27
04/09/2009
28
25.6
8.11
05/09/2009
29
24,9
06/09/2009
30
07/09/2009
31
08/09/2009
32
24.6
8.13
SC Hardness Alkailinity Ammonia
7.6 356 0 144.0 104.0 ND
8.0 358.0 ND
8.0
ND
7.6 369.0
7.6
7.7
7.4
7.0 347.0 ND
7.9
7.6
7.1 366.0 128.0 106.0 ND
N
18
11
11
5
2
2
Max
25.8
8.27
8.0
369.0
144.0
106.0
Min
24,1
8.05
7.0
347.0
128.0
104.0
Mean
25,1
8,18
7.6
359.2
136.0
105.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
25.0 mg/L Nitrate Rep 2
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
07/08/2009
0
25.1
8.20
7.9
08/08/2009
1
25.1
09/08/2009
2
10/08/2009
3
25.2
11/08/2009
4
25.7
12/08/2009
5
25.2
8.31
8.0
13/08/2009
6
14/08/2009
7
25.1
8.30
9,1
15/08/2009
8
25.1
16/08/2009
9
17/08/2009
10
25.4
8.18
7.5
18/08/2009
11
19/08/2009
12
25.1
8.55
8.0
20/08/2009
13
21/08/2009
14
25.5
8.23
7.6
22/08/2009
15
23/08/2009
16
25,9
24/08/2009
17
25,0
8.25
7.7
25/08/2009
18
26/08/2009
19
27/08/2009
20
25.1
28/08/2009
21
29/08/2009
22
24.7
30/08/2009
23
24,2
31/08/2009
24
01/09/2009
25
02/09/2009
26
03/09/2009
27
24.7
04/09/2009
28
25.7
8.04
7.3
05/09/2009
29
06/09/2009
30
25.6
07/09/2009
31
25,5
08/09/2009
32
N
19
8
8
1
Max
25,9
8,55
9,1
368.0
Min
24.2
8.04
7,3
368.0
Mean
25.2
8.26
7.9
368,0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
25.0 mg/L Nitrate Rep 3
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
8.07 7.8
07/08/2009
0
25.0
08/08/2009
1
09/08/2009
2
25.7
10/08/2009
3
11/08/2009
4
12/08/2009
5
24.7
13/08/2009
6
24.7
14/08/2009
7
15/08/2009
8
16/08/2009
9
25.0
17/08/2009
10
25.2
18/08/2009
11
25.6
19/08/2009
12
20/08/2009
13
25.7
21/08/2009
14
22/08/2009
15
24.6
23/08/2009
16
24/08/2009
17
24.6
25/08/2009
18
25.2
26/08/2009
19
25.5
27/08/2009
20
28/08/2009
21
25.1
29/08/2009
22
30/08/2009
23
31/08/2009
24
01/09/2009
25
24.7
02/09/2009
26
24.7
03/09/2009
27
24.3
04/09/2009
28
05/09/2009
29
06/09/2009
30
25.5
07/09/2009
31
25.3
08/09/2009
32
8.25 7.9
ND
8.21 8.0
8.23 7.9
8.15 7.4
N
18
5
5
Max
25.7
8.25
8.0
Min
24.3
8.07
7.4
Mean
25.1
8.18
7.8
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
25.0 mg/L Nitrate Rep 4
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
8.16 7.8 ND
8.32 8.0 360.0 ND
8.33 9.1
ND
8.50 8.1
07/08/2009
0
24.9
08/08/2009
1
09/08/2009
2
10/08/2009
3
24.9
11/08/2009
4
26.1
12/08/2009
5
24.5
13/08/2009
6
24.6
14/08/2009
7
25.3
15/08/2009
8
16/08/2009
9
25.6
17/08/2009
10
25.2
18/08/2009
11
25.4
19/08/2009
12
25.2
20/08/2009
13
21/08/2009
14
22/08/2009
15
24.7
23/08/2009
16
24/08/2009
17
25/08/2009
18
25.2
26/08/2009
19
27/08/2009
20
25.1
28/08/2009
21
29/08/2009
22
24.4
30/08/2009
23
31/08/2009
24
24.3
01/09/2009
25
24.6
02/09/2009
26
03/09/2009
27
04/09/2009
28
05/09/2009
29
25.3
06/09/2009
30
07/09/2009
31
08/09/2009
32
24.4
8.05 6.9 342.0 ND
8.02 6.4 370.0 132.0 106.0 ND
N
18
6
6
3
1
1
Max
26.1
8.50
9.1
370.0
132.0
106.0
Min
24.3
8.02
6.4
342.0
132.0
106.0
Mean
25.0
8.23
7.7
357.3
132.0
106.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
50.0 mg/L Nitrate Rep 1
'ate
Test Day
Temperature
pH DO
SC Hardness
Alkallinity Ammonia
07/08/2009
0
24.7
8.25
8.0
419.0 156.0
104.0 ND
08/08/2009
1
25.2
09/08/2009
2
25.6
10/08/2009
3
25.2
8.28
8.1
418.0
ND
11/08/2009
4
12/08/2009
5
24.7
8.30
8.0
13/08/2009
6
25.0
14/08/2009
7
25.4
8.34
9.1
15/08/2009
8
25.0
16/08/2009
9
ND
17/08/2009
10
24.8
8.17
7.7
426.0
18/08/2009
11
19/08/2009
12
20/08/2009
13
25.1
21/08/2009
14
25.5
8.26
8.1
22/08/2009
15
23/08/2009
16
26.0
24/08/2009
17
25/08/2009
18
26/08/2009
19
25.3
8.23
7.9
27/08/2009
20
28/08/2009
21
25.0
8.24
7,6
29/08/2009
22
30/08/2009
23
24.1
31/08/2009
24
24.4
8.06
7.4
396.0
ND
01/09/2009
25
02/09/2009
26
24.0
8.16
7.8
03/09/2009
27
04/09/2009
28
25.7
8.13
7.6
05/09/2009
29
25.5
06/09/2009
30
07/09/2009
31
08/09/2009
32
24.6
8.14
7.2
426.0 132.0
104.0 ND
N
20
12
12
5
2
2
Max
26.0
8.34
9.1
426.0
156.0
104.0
Mirt
24.0
8.06
7.2
396.0
132.0
104.0
Mean
25.0
8.21
7.9
417.0
144.0
104.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
50.0 mg/L Nitrate Rep 2
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
07/08/2009
0
25,2
8.17
8.0
08/08/2009
1
25.0
09/08/2009
2
10/08/2009
3
24,7
11/08/2009
4
25,8
12/08/2009
5
24.7
8.34
8.0
13/08/2009
6
14/08/2009
7
15/08/2009
8
25.2
16/08/2009
9
17/08/2009
10
24.9
8.16
7.7
18/08/2009
11
19/08/2009
12
25.2
8.56
8,3
20/08/2009
13
21/08/2009
14
24,2
8.27
7.9
22/08/2009
15
23/08/2009
16
25.7
24/08/2009
17
24.7
8.23
7.6
25/08/2009
18
26/08/2009
19
27/08/2009
20
25.2
28/08/2009
21
29/08/2009
22
24.6
30/08/2009
23
24.4
31/08/2009
24
01/09/2009
25
02/09/2009
26
03/09/2009
27
24.3
04/09/2009
28
24.7
8.18
7.9
05/09/2009
29
06/09/2009
30
25.3
07/09/2009
31
25.3
08/09/2009
32
N
18
7
7
1
Max
25.8
8.56
8.3
431.0
Min
24.2
8.16
7.6
431.0
Mean
25.0
8.27
7.9
431.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
50.0 mg/L Nitrate Rep 3
Date Test Day Temperature pH DO SC Hardness Alkaliinity Ammonia
8.10 8.0
07/08/2009
0
25.2
08/08/2009
1
09/08/2009
2
25.7
10/08/2009
3
11/08/2009
4
12/08/2009
5
25.0
13/08/2009
6
24.9
14/08/2009
7
15/08/2009
8
16/08/2009
9
24.4
17/08/2009
10
24.8
18/08/2009
11
25.7
19/08/2009
12
20/08/2009
13
24.9
21/08/2009
14
22/08/2009
15
24.5
23/08/2009
16
24/08/2009
17
24.6
25/08/2009
18
25.2
26/08/2009
19
25.5
27/08/2009
20
28/08/2009
21
24.9
29/08/2009
22
30/08/2009
23
31/08/2009
24
01/09/2009
25
24,7
02/09/2009
26
24,7
03/09/2009
27
24.3
04/09/2009
28
05/09/2009
29
06/09/2009
30
07/09/2009
31
25.3
08/09/2009
32
8.27 7,7
ND
8.18 76
8.23 7.7
8.15 7,7
N
17
5
5
Max
25,7
8.27
8.0
Min
24.3
8.10
7.6
Mean
25.0
8.19
7.7
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
50,0 mg/L Nitrate Rep 4
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
8.20 8.0 ND
8.40 8.1 428.0 ND
8.31 9.2
ND
8.57 8,4
07/08/2009
0
25.0
08/08/2009
1
09/08/2009
2
25.7
10/08/2009
3
25.6
11/08/2009
4
26.1
12/08/2009
5
24.7
13/08/2009
6
14/08/2009
7
25.9
15/08/2009
8
16/08/2009
9
25,0
17/08/2009
10
24,8
18/08/2009
11
25.6
19/08/2009
12
25.6
20/08/2009
13
21/08/2009
14
22/08/2009
15
24.8
23/08/2009
16
24/08/2009
17
25/08/2009
18
25.6
26/08/2009
19
27/08/2009
20
25.2
28/08/2009
21
29/08/2009
22
25.3
30/08/2009
23
31/08/2009
24
24.5
01/09/2009
25
25.6
02/09/2009
26
03/09/2009
27
04/09/2009
28
05/09/2009
29
25.3
06/09/2009
30
25.4
07/09/2009
31
08/09/2009
32
25.2
8.05 7.0 420,0 ND
8,22 7,4 430.0 124.0 104.0 ND
N
19
6
6
3
1
1
Max
26.1
8.57
9.2
430,0
124.0
104.0
Mill
24.5
8.05
7.0
420.0
124.0
104,0
Mean
25.3
8.29
8.0
426.0
124.0
104.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
100 mg/L Nitrate Rep 1
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
495.0 144.0 104,0 ND
536.0 ND
07/08/2009
0
24,4
8,17
8.0
08/08/2009
1
24.7
09/08/2009
2
10/08/2009
3
24.7
8.30
8.0
11/08/2009
4
12/08/2009
5
24.7
8.32
8.0
13/08/2009
6
24.4
14/08/2009
7
24.4
8.32
9.4
15/08/2009
8
25.0
16/08/2009
9
17/08/2009
10
25.1
8.18
7.7
18/08/2009
11
19/08/2009
12
20/08/2009
13
25.4
21/08/2009
14
24.0
8.27
7,9
22/08/2009
15
23/08/2009
16
24.9
24/08/2009
17
25/08/2009
18
26/08/2009
19
24,8
8.22
8.0
27/08/2009
20
28/08/2009
21
24,5
8.28
8.0
29/08/2009
22
30/08/2009
23
24.1
31/08/2009
24
24,5
8,15
7.4
01/09/2009
25
02/09/2009
26
24.0
8.23
8.0
03/09/2009
27
04/09/2009
28
25.0
8.24
8.1
05/09/2009
29
25.3
06/09/2009
30
07/09/2009
31
08/09/2009
32
24.4
8,23
7.6
ND
544.0
530.0 ND
537.0 128.0 102.0 ND
N
19
12
12
5
2
2
Max
25.4
8.32
9.4
544.0
144.0
104.0
Min
24.0
8.15
7.4
495.0
128.0
102.0
Mean
24.6
8.24
8.0
528,4
136.0
103.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
100 mg/L Nitrate Rep 2
Date Test Day Temperature pH DO SC Hardness Alkaliinity Ammonia
07/08/2009
0
25.2
8.21
8.1
08/08/2009
1
24.7
09/08/2009
2
25.4
10/08/2009
3
11/08/2009
4
26.3
12/08/2009
5
24,7
8,27
8.0
13/08/2009
6
24,5
14/08/2009
7
24.3
8.34
9.0
15/08/2009
8
25.1
16/08/2009
9
17/08/2009
10
25.2
8.18
7.5
18/08/2009
11
19/08/2009
12
25,7
8.37
8.2
20/08/2009
13
21/08/2009
14
24.4
8,26
8.0
22/08/2009
15
23/08/2009
16
25.2
24/08/2009
17
24,8
8.32
7.9
25/08/2009
18
26/08/2009
19
27/08/2009
20
25.2
28/08/2009
21
29/08/2009
22
24.7
30/08/2009
23
24.3
31/08/2009
24
01/09/2009
25
02/09/2009
26
03/09/2009
27
24.4
04/09/2009
28
25.5
8.25
8.1
05/09/2009
29
06/09/2009
30
25.5
07/09/2009
31
25.2
08/09/2009
32
N
20
8
8
1
Max
26.3
8.37
9.0
545.0
Min
24,3
8.18
7.5
545.0
Mean
25,0
8,28
8.1
545.0
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
100 mg/L Nitrate Rep 3
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
8.10 8,1
07/08/2009
0
25.4
08/08/2009
1
09/08/2009
2
25.7
10/08/2009
3
24.7
11/08/2009
4
12/08/2009
5
24.7
13/08/2009
6
14/08/2009
7
15/08/2009
8
16/08/2009
9
24.4
17/08/2009
10
25.2
18/08/2009
11
25.8
19/08/2009
12
20/08/2009
13
25.7
21/08/2009
14
22/08/2009
15
24.5
23/08/2009
16
24/08/2009
17
24,7
25/08/2009
18
25.1
26/08/2009
19
24.8
27/08/2009
20
28/08/2009
21
24,5
29/08/2009
22
30/08/2009
23
31/08/2009
24
01/09/2009
25
24.6
02/09/2009
26
24.5
03/09/2009
27
24.3
04/09/2009
28
05/09/2009
29
06/09/2009
30
25.5
07/09/2009
31
25.2
08/09/2009
32
8.33 8.0
ND
8.23 8.0
8.31 8.0
8.27 8,2
N
18
5
5
Max
25.8
8.33
8.2
Min
24.3
8.10
8.0
Mean
25.0
8.25
8.1
-------
Daily Chemistry Data for the 32-Day Toxicity Test
HECD B-12 Nitrite
Pimephales promelas (Fathead Minnow)
100 mg/L Nitrate Rep 4
Date Test Day Temperature pH DO SC Hardness Alkallinity Ammonia
07/08/2009 0 25,4 8.16 7.8 ND
08/08/2009 1
09/08/2009 2 25,6
10/08/2009 3 24.8 8.29 8.0 529.0 ND
11/08/2009 4 26.2
12/08/2009 5 25.1
13/08/2009 6
14/08/2009 7
15/08/2009 8
16/08/2009 9 25.1 ND
17/08/2009 10 25.2
18/08/2009 11 25.6
19/08/2009 12 25.4 8.72 8.3
20/08/2009 13
21/08/2009 14
22/08/2009 15 24.5
23/08/2009 16
24/08/2009 17
25/08/2009 18 25.3
26/08/2009 19
27/08/2009 20 24,9
28/08/2009 21
29/08/2009 22 24.9
30/08/2009 23
31/08/2009 24 24.5 8.12 7.2 510.0 ND
01/09/2009 25 25.1
02/09/2009 26
03/09/2009 27
04/09/2009 28
05/09/2009 29 25.2
06/09/2009 30
07/09/2009 31
08/09/2009 32 24.6 8.14 7.1 324.0 132.0 104.0 ND
N
17
5
5
3
1
1
Max
26,2
8 72
8.3
529.0
132.0
104.0
Min
24.5
8.12
7.1
324.0
132.0
104.0
Mean
25.1
8.29
7.7
454.3
132.0
104.0
-------
HECD B-12 32-Day FHM Chronic Toxicity Test
with Nitrite
o
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrite for HECD B-12 with Pimephales promela (fathead minnow)
Date/Time Temperatu
08/19/09 0
25.16
08/19/091
25.33
08/19/09 1
25.33
08/19/091
25.33
08/19/091
25.5
08/19/091
25.33
08/19/09 1
25.5
08/19/09 1
25.5
08/19/09 1
25.5
08/19/091
25.33
08/19/091
25.5
08/19/09 2
25.67
08/19/09 2
25.5
08/19/09 2
25.5
08/19/09 2
25.5
08/20/09 0
25.5
08/20/09 0
25.5
08/20/09 0
25,5
08/20/09 0
25.33
08/20/09 0
25.5
08/20/09 0
25.5
08/20/09 0
25,5
08/20/09 0
25.5
08/20/09 0
25,5
08/20/09 0
25.33
08/20/09 1
25,16
08/20/09 1
25.16
08/20/09 1
25.16
08/20/09 1
25.16
08/20/09 1
25,16
08/20/09 1
25.16
08/20/09 1
25.33
08/20/09 1
25.16
08/20/09 1
25.33
08/20/09 1
25.5
08/20/09 2
25.5
08/20/09 2
25.33
08/20/09 2
25.5
08/20/09 2
25.33
08/21/09 0
25.33
08/21/09 0
25.33
08/21/09 0
25.33
08/21/09 0
25.33
08/21/08 0
25.33
08/21/09 0
25.16
08/21/09 0
25.33
08/21/09 0
25,33
08/21/09 0
25.5
08/21/09 0
25.16
08/21/09 1
24.98
08/21/09 1
24.98
08/21/09 1
24.98
08/21/09 1
24.98
08/21/09 1
24.98
08/21/09 1
24.81
08/21/09 1
24.81
08/21/09 1
24.81
08/21/09 1
24.98
08/21/09 1
24.81
08/21/09 2
24.98
08/21/09 2
24.81
08/21/09 2
24.98
1 of 8
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrite for HECD B-12 with Pimephales promela (fathead minnow)
Date/Time Temperatu
08/21/09
2
24.81
08/22/09
0
24.81
08/22/09
0
24.81
08/22/09
0
24.81
08/22/09
0
24.81
08/22/09
0
24.98
08/22/09
0
24.98
08/22/09
0
24.98
08/22/09
0
24.98
08/22/09
0
24.98
08/22/09
0
24.98
08/22/09
1
25.16
08/22/09
1
25,16
08/22/09
1
25.16
08/22/09
1
25.16
08/22/09
1
25.5
08/22/09
1
25.33
08/22/09
1
25,33
08/22/09
1
25,33
08/22/09
1
25,33
08/22/09
1
25.33
08/22/09
2
25,33
08/22/09
2
25,33
08/22/09
2
25,16
08/22/09
2
25,16
08/23/09
0
25,16
08/23/09
0
25.16
08/23/09
0
25.16
08/23/09
0
25.16
08/23/09
0
25.33
08/23/09
0
25,33
08/23/09
0
25,33
08/23/09
0
25.33
08/23/09
0
25.33
08/23/09
0
25.33
08/23/09
25,16
08/23/09
25,33
08/23/09
25,33
08/23/09
25,16
08/23/09
25,16
08/23/09
25,16
08/23/09
25,16
08/23/09
25.16
08/23/09
25.33
08/23/09
25.33
08/23/09
2
25.33
08/23/09
2
25,16
08/23/09
2
25,16
08/23/09
2
25,16
08/24/09
0
24,98
08/24/09
0
25.16
08/24/09
0
24.98
08/24/09
0
25.16
08/24/09
0
25.16
08/24/09
0
24,98
08/24/09
0
25.16
08/24/09
0
25,16
08/24/09
0
25.16
08/24/09
0
25.16
08/24/09
1
25.16
08/24/09
1
25.16
08/24/09
1
25.16
2 Of 8
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrite for HECD B-12 with Pimephales promela (fathead minnow)
Date/Time Temperatu
08/24/09 1
25.16
08/24/09 1
25.16
08/24/09 1
25.16
08/24/09 1
25.16
08/24/09 1
25.33
08/24/09 1
25.16
08/24/09 1
25.5
08/24/09 2
25.5
08/24/09 2
25.33
08/24/09 2
25.33
08/24/09 2
25.33
08/25/09 0
25.33
08/25/09 0
25.33
08/25/09 0
25.16
08/25/09 0
25.33
08/25/090
25.16
08/25/09 0
25.33
08/25/09 0
25.5
08/25/09 0
25.5
08/25/09 0
25.33
08/25/09 0
25.33
08/25/09 1
25.16
08/25/09 1
25.33
08/25/09 1
25.33
08/25/09 1
25.16
08/25/09 1
25.33
08/25/09 1
25.33
08/25/09 1
25.33
08/25/09 1
25,16
08/25/09 1
25.33
08/25/09 1
25.33
08/25/09 2
25.16
08/25/09 2
25.33
08/25/09 2
25.33
08/25/09 2
25.16
08/26/09 0
25.33
08/26/09 0
25.33
08/26/09 0
25.33
08/26/09 0
25.16
08/26/09 0
25.33
08/26/09 0
25.33
08/26/09 0
25.33
08/26/09 0
25.33
08/26/09 0
25,33
08/26/09 0
25,33
08/26/09 1
25.16
08/26/09 1
25,16
08/26/09 1
25.16
08/26/09 1
25,16
08/26/09 1
25.16
08/26/09 1
25.16
08/26/09 1
25.16
08/26/09 1
25.16
08/26/09 1
25.33
08/26/09 1
25.33
08/26/09 2
25.33
08/26/09 2
25.33
08/26/09 2
25.33
08/26/09 2
25.16
08/27/09 0
25.33
08/27/09 0
25.33
08/27/09 0
25.16
3 of 8
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrite for HECD B-12 with Pimephales promela (fathead minnow)
Date/Time Temperatu
08/27/09
0
25.18
08/27/09
0
25.16
08/27/09
0
25.16
08/27/09
0
25.16
08/27/09
0
25.5
08/27/09
0
25.5
08/27/09
0
25.33
08/27/09
1
25.16
08/27/09
1
25.33
08/27/09
1
25.16
08/27/09
1
25,33
08/27/09
1
25,33
08/27/09
1
25,16
08/27/09
1
25,16
08/27/09
1
25.16
08/27/09
1
25.16
08/27/09
1
25.33
08/27/09
2
25.16
08/27/09
2
25,16
08/27/09
2
25,16
08/27/092
25,18
08/28/090
24.98
08/28/09
0
24.98
08/28/09
0
24.98
08/28/09
0
24.98
08/28/09
0
24.98
08/28/09
0
24.98
08/28/09
0
25.33
08/28/09
0
25.16
08/28/09
0
25.16
08/28/09
0
25.16
08/28/09
1
25.16
08/28/09
1
25.16
08/28/09
1
25.16
08/28/09
1
25.16
08/28/09
1
25.16
08/28/09
1
25.16
08/28/09
1
25.16
08/28/09
1
25.16
08/28/09
1
25.16
08/28/09
1
24.98
08/28/09
2
25.16
08/28/09
2
25.16
08/28/09
2
24.98
08/28/09
2
25.16
08/29/09
0
24.98
08/29/09
0
24,98
08/29/09
0
24,98
08/29/09
0
24,98
08/29/09
0
25,16
08/29/09
0
25.16
08/29/09
0
25.16
08/29/09
0
25.16
08/29/09
0
25.16
08/29/09
0
25.33
08/29/09
1
25.16
08/29/09
1
25.16
08/29/09
1
25.16
08/29/09
1
24.98
08/29/09
1
25.16
08/29/09
1
25.16
08/29/09
1
25,16
4 of 8
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrite for HECD B-12 with Pimephales promela (fathead minnow)
Date/Time Temperatu
08/29/09
1
25,16
08/29/09
1
25.16
08/29/09
1
25.16
08/29/09
2
24.98
08/29/09
2
24.98
08/29/09
2
24.98
08/29/09
2
25.16
08/30/09
0
25.16
08/30/09
0
24.98
08/30/09
0
24.98
08/30/09
0
24.98
08/30/09
0
24.98
08/30/09
0
24.98
08/30/09
0
24.98
08/30/09
0
25.16
08/30/09
0
25.16
08/30/09
0
25.16
08/30/09
25.16
08/30/09
25.16
08/30/09
24.98
08/30/09
25,16
08/30/09
25.16
08/30/09
25.16
08/30/09
25.16
08/30/09
1
25.16
08/30/09
1
25.16
08/30/09
1
25.33
08/30/09
2
25.33
08/30/09
2
25.16
08/30/09
2
25.16
08/30/09
2
25.16
08/31/09
0
25.16
08/31/09
0
25.16
08/31/09
0
25.16
08/31/09
0
24.98
08/31/09
0
25.16
08/31/09
0
25.16
08/31/09
0
25,16
08/31/09
0
25.16
08/31/09
0
25.33
08/31/09
0
24.98
08/31/09
1
24.98
08/31/09
1
25.16
08/31/09
1
24.81
08/31/09
1
24.98
08/31/09
1
25.33
08/31/09
1
25.33
08/31/09
1
25.5
08/31/09
1
25.67
08/31/09
1
25.67
08/31/09
1
25.67
08/31/09
2
25,84
08/31/09
2
25.84
08/31/09
2
25,67
08/31/09
2
25,67
09/01/09
0
25,84
09/01/09
0
25,67
09/01/09
0
25.5
09/01/09
0
25,67
09/01/09
0
25.5
09/01/09
0
25.67
09/01/09
0
25.67
5 of 8
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrite for HECD B-12 with Pimephaies promela (fathead minnow)
Date/Time Temperatu
09/01/09 0
25.67
09/01/09 0
25.84
09/01/09 0
24.81
09/01/09 1
24.81
09/01/09 1
24.81
09/01/09 1
24.98
09/01/09 1
24.98
09/01/09 1
24.81
09/01/09 1
24.81
09/01/09 1
25.16
09/01/09 1
25.16
09/01/09 1
25.16
09/01/09 1
25.16
09/01/09 2
25.16
09/01/09 2
25.16
09/01/09 2
24.98
09/01/09 2
25.16
09/02/09 0
25.16
09/02/09 0
25,16
09/02/09 0
25.16
09/02/09 0
25.16
09/02/09 0
25.16
09/02/09 0
25.16
09/02/09 0
24.98
09/02/09 0
25.16
09/02/09 0
25.16
09/02/09 0
25,16
09/02/09 1
24.98
09/02/09 1
24.98
09/02/09 1
24.81
09/02/09 1
24.64
09/02/09 1
24.64
09/02/09 1
24.64
09/02/09 1
24,98
09/02/09 1
24.98
09/02/09 1
24.98
09/02/09 1
24.98
09/02/09 2
24.98
09/02/09 2
24.98
09/02/09 2
24.98
09/02/09 2
24.98
09/03/09 0
24.98
09/03/09 0
24.98
09/03/09 0
24,98
09/03/09 0
24,98
09/03/09 0
24.81
09/03/09 0
24.98
09/03/09 0
24.98
09/03/09 0
24,98
09/03/09 0
24.98
09/03/09 0
24.81
09/03/09 1
24.64
09/03/09 1
24.81
09/03/09 1
24,81
09/03/09 1
24,81
09/03/09 1
24.81
09/03/09 1
24.81
09/03/09 1
24.64
09/03/09 1
24.81
09/03/09 1
24.81
09/03/09 1
24.98
09/03/09 2
25,16
6 of 8
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrite for HECD B-12 with Pimephales promeia (fathead minnow)
Date/Time Temperatu
09/03/09 2
25.16
09/03/09 2
25.16
09/03/09 2
24,98
09/04/09 0
25.16
09/04/09 0
25.16
09/04/09 0
25.16
09/04/09 0
25,16
09/04/09 0
25,16
09/04/09 0
25.16
09/04/09 0
25.16
09/04/09 0
25.16
09/04/09 0
25,16
09/04/09 0
24.98
09/04/09 1
24.98
09/04/09 1
25,16
09/04/09 1
24.98
09/04/09 1
24.98
09/04/09 1
25.16
09/04/09 1
24.98
09/04/09 1
24.98
09/04/09 1
25.16
09/04/09 1
25.33
09/04/09 1
25.33
09/04/09 2
25,33
09/04/09 2
25.33
09/04/09 2
25.33
09/04/09 2
25.33
09/05/09 0
25,16
09/05/09 0
25.33
09/05/09 0
25.16
09/05/09 0
25.16
09/05/09 0
25.16
09/05/09 0
25.16
09/05/09 0
25.16
09/05/09 0
25.16
09/05/09 0
25.33
09/05/09 0
25.33
09/05/09 1
25.16
09/05/09 1
25.16
09/05/09 1
25.16
09/05/09 1
24.98
09/05/09 1
25.16
09/05/09 1
24.98
09/05/09 1
24.98
09/05/09 1
24.98
09/05/09 1
25.16
09/05/09 1
25,16
09/05/09 2
25.16
09/05/09 2
25.16
09/05/09 2
24.98
09/05/09 2
24.98
09/06/09 0
24.98
09/06/09 0
24.98
09/06/09 0
24.98
09/06/09 0
24,98
09/06/09 0
24,98
09/06/09 0
24.81
09/06/09 0
24.98
09/06/09 0
24.98
09/06/09 0
24.98
09/06/09 0
24.98
09/06/09 1
25.16
7 of 8
-------
32-Day Early Life Stage Flow Through Toxicity of Nitrite for HECD B-12 with Pimephales promela {fathead minnow)
Date/Time Temperatu
09/06/09 1
24.98
09/06/09 1
24.98
09/06/09 1
24.98
09/06/09 1
25.16
09/06/09 1
25.16
09/06/09 1
25,16
09/06/09 1
25.16
09/06/09 1
25.16
09/06/09 1
25.16
09/06/09 2
25.16
09/06/09 2
25.16
09/06/09 2
25.16
09/06/09 2
25.16
09/07/09 0
25.16
09/07/09 0
25.16
09/07/09 0
24.98
Ofl/07/09 0
25.16
09/07/09 0
25.16
09/07/09 0
24.98
09/07/09 0
24.98
09/07/09 0
25.16
09/07/09 0
25.16
09/07/09 0
25.16
09/07/09 1
25.16
09/07/09 1
25.16
09/07/09 1
24.18
09/07/09 1
25.16
09/07/09 1
25.16
09/07/09 1
25.16
09/07/09 1
25.16
09/07/09 1
25.16
09/07/09 1
25.16
09/07/09 1
25.16
09/07/09 2
25.16
09/07/09 2
25.16
09/07/09 2
25.16
09/07/09 2
24.98
09/08/09 0
24.98
09/08/09 0
24,98
09/08/09 0
25.16
09/08/09 0
24.98
09/08/09 0
24.98
09/08/09 0
24.98
09/08/09 0
24.98
09/08/09 0
24.98
09/08/09 0
25.16
09/08/09 0
24.81
09/08/09 1
24,98
09/08/09 1
24.81
09/08/09 1
24.81
09/08/09 1
24.81
09/08/09 1
24.81
09/08/09 1
24.81
09/08/09 1
24,81
09/08/09 1
24,81
09/08/09 1
24.98
09/08/09 1
24.98
8 of 8
-------
Statistical Analyses
-------
TRIMMED SPEARMAN-KARBER METHOD, MONTANA STATE UNIV
FOR REFERENCE, CITE;
HAMILTON, M.A., R.C, RUSSO, AND R.V. THURSTON, 1977.
TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING MEDIAN
LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS,
ENVIRON. SCI. TECHNOL. 11(7) : 714-719;
CORRECTION 12(4);417 (1978),
DATE: 8/3/09 TEST NUMBER; 6112-12
CHEMICAL: NITRITE SPECIES: FHM
RAW DATA;
CONCENTRATION(MG/L
NUMBER EXPOSED:
5.53 11,30 22.50 49.20 99.30
80 80 80 80 80
DURATION {DAYS
LC5G LOWER 95% LIMIT UPPER 95% LIMIT PERCENT
TRIM
32
37 , 13
31.85
43 ,28
11.25
-------
Title; Nitrite FHM 32-day life stage survival sept 2009
File: no2fhs32 Transform: ARC SINE(SQUARE ROOT(Y))
Shapiro - Wilk's Test for Normality
D = 0 .5672
W = 0.9331
Critical W = 0.8680 (alpha = 0.01 , N = 20)
W = 0.9050 (alpha = 0.05 , N = 20)
Data PASS normality test (alpha = o.oi). Continue analysis.
Title: Nitrite FHM 3 2-day life stage survival sept 2009
File: no2fhs32 Transform: ARC SINE(SQUARE ROOT(Y))
Hartley's Test for Homogeneity of Variance
Calculated H statistic (max Var/min Var) = 2.2636
Table H statistic = 151.00 (alpha = 0.01)
50.70 (alpha = 0.05)
Used df = 3
Based on R (# groups) = 5
Data PASS homogeneity test (alpha = 0.01). Continue analysis.
Title: Nitrite FHM 32-day life stage survival sept 2009
File: no2fhs32 Transform: ARC SINE(SQUARE ROOT(Y))
Bartlett's Test for Homogeneity of Variance
Calculated B1 statistic = 0.7630 (p-value = 0.9433)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B = 13.2767 (alpha = 0.01, df =
= 9.4877 (alpha = 0.05, df =
4)
4)
-------
Title
File;
Numbe
GR
1
1
1
1
2
2
2
2
3
3
3
3
4
4
4
4
5
5
5
5
Title
File:
Title
Pile;
Nitrite FHM 32-day life 'stage survival sept 2009
no2fhs32 Transform: ARC SINE{SQUARE ROOT(Y) )
of Groups: 5
IDENTIFICATION REP VALUE TRANS VALUE
control
1
1
0000
1.4588
control
2
0
9500
1.34 53
control
3
0
6500
1. 1731
control
4
0
8000
1.1071
5,53 mg/L
1
0
8000
1,1071
5,53 mg/L
2
0
8500
1.1731
5.53 mg/L
3
0
9000
1.2490
5.53 mg/L
4
1
0000
1 .4588
11.3 mg/L
1
0
9500
1.3453
11.3 mg/L
2
0
6000
0.8861
11.3 mg/L
3
0
9500
1.3453
11.3 mg/L
4
0
9500
1.3453
22.5 mg/L
1
0
8000
1.1071
22.5 mg/L
2
0
8000
1.1071
22.5 mg/L
3
0
9500
1.3453
22.5 mg/L
4
0
6000
0.8851
4 9.2 mg/L
1
0
4500
0.7353
4 9.2 mg/L
2
0
1000
0.3218
4 9.2 mg/L
3
0
4500
0.7353
49.2 mg/L
4
0
5500
0.8355
Nitrite FHM 32-day life stage survival sept 2009
no2fhs32 Transform: ARC SINE(SQUARE ROOT(Y)}
Summary Statistics oil Transformed Data TABLE I of 2
GRP IDENTIFICATION N¦ MIN MAX MEAN
1
control
4
1.1071
1.4588
1.2711
2
5.53 mg/L
4
1.1071
1.4588
1.2470
3
11.3 mg/L
4
0.8861
1.3453
1,2305
4
22.5 mg/L
4
0.8861
1.3453
1.1114
5
49.2 mg/L
4
0.3218
0.8355
0,6570
Nitrite FHM 32-day life stage survival sept 2009
no2fhs32 Transform: ARC SINE(SQUARE ROOT(Y))
Summary Statistics on Transformed Data TABLE 2 of 2
IDENTIFICATION VARIANCE SD SEM C.V. %
-------
1 control 0.0257 0.1604 0.0802 12.6209
2 5.53 mg/L 0.0233 0.1526 0.0763 12.2379
3 11.3 mg/L 0.0527 0.2296 0.1148 18.6596
4 22.5 mg/L 0.0352 0.1875 0.0938 16.8735
5 49.2 mg/L 0.0522 0.2284 0.1142 34.7675
Title: Nitrite FHM 32-day life stage survival sept 2009
File: no2fhs32 Transform: ARC SINE (SQUARE ROOT (1) )
ANOVA Table
SOURCE
DF
SS
MS
F
Between
4
1.0570
0.2643
6.9879
Within (Error)
15
0.5672
0.0378
Total
19
1.6243
(p-value = 0.0022)
Critical F = 4.8932 (alpha = 0.01, df = 4,15)
= 3.0556 (alpha = 0.05, df «. 4,15)
Since F > Critical F REJECT Ho: All equal (alpha = 0.05)
Title: Nitrite FHM 32-day life stage survival sept 2009
File: no2fhs32 Transform: ARC SINE(SQUARE ROOT(Y))
Dunnett's Test
TABLE 1 OF 2
Ho -• Control
-------
1 control 4
2 5.53 mg/L 4
3 11.3 mg/L 4
4 22.5 mg/L 4
5 49.2 mg/L 4
0.2544 27.9 0.0125
0.2544 27.9 0.0375
0.2544 27.9 0.1125
0.2544 27.9 0,5125
-------
Title: Nitrite FHM 32-day life stage growth sept 2009
File: N02FHG32. Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
D = 8.4584
W = 0.8946
Critical W = 0.8680 {alpha =0.01 , N = 20)
W = 0.9050 {alpha = 0.05 , N = 20)
Data PASS normality test (alpha = 0.01). Continue analysis.
Title: Nitrite FHM 32-day life stage growth sept 2009
File: N02FHG32. Transform: NO TRANSFORMATION
Hartley's Test for Homogeneity of Variance
Calculated H statistic {max Var/min Var) = 18.4501
Table H statistic = 151.00 (alpha = 0.01)
50.70 (alpha = 0.05)
Used df = 3
Based on R (# groups) = 5
Data PASS homogeneity test (alpha = 0.01). Continue analysis.
Title: Nitrite FHM 32-day life stage growth sept 2009
File: N02FHG3 2. Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated 31 statistic = 8.9973 (p-value = 0.0612)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B = 13.2767 {alpha = 0.01, df = 4)
= 9.4877 (alpha = 0.05, df = 4)
-------
Title: Nitrite FHM 32-day life stage growth sept 2009
File; N02FHG32. Transform: NO TRANSFORMATION
Number of Groups: 5
GRP IDENTIFICATION REP VALUE TRANS VALUE
1
control
1
5.7200
5.7200
1
control
2
6.2800
6.2800
1
control
3
5.7300
5.7300
1
control
4
6.3300
6.3300
2
5 .
53
mg/L
1
6.8600
6.8600
2
5 .
53
mg/L
2
5.6900
5.6900
2
5 .
53
mg/L
3
5.7700
5.7700
2
5 ,
53
mg/L
4
6.1200
6.1200
3
11
,3
mg/L
1
6.3500
6.3500
3
11
,3
mg/L
2
7.0400
7,0400
3
11
.3
mg/L
3
6.2600
6,2600
3
11
. 3
mg/L
4
6.2800
6.2800
4
22
. 5
mg/L
1
6.5500
6.5500
4
22
. S
mg/L
2
6.2800
6.2 800
4
22
,5
mg/L
3
5.5100
5.5100
4
22
,5
mg/L
4
6.3400
6.3400
5
49
. 2
mg/L
1
3.2700
3.2700
5
49
,2
mg/L
2
0.8800
0.8800
5
49
. 2
mg/L
3
3.4300
3.4300
5
49
.2
mg/L
4
4.2200
4.2200
Title: Nitrite FHM 32-day life stage growth sept 2009
File: N02FHG32. Transform: NO TRANSFORMATION
Summary Statistics on Data TABLE 1 of 2
GRP IDENTIFICATION N MIN MAX MEAN
1
control
4
5.7200
6.3300
6.0150
2
5,53 mg/L
4
5.6900
6.8600
6.1100
3
11.3 mg/L
4
6.2600
7.0400
6.4825
4
22.5 mg/L
4
5.5100
6.5500
6.1700
5
49.2 mg/L
4
0.8800
4 . 2200
2.9500
Title: Nitrite FHM 32-day life stage growth sept 2009
File: N02FHG32. Transform: NO TRANSFORMATION
Summary Statistics on Data TABLE 2 of 2
GRP IDENTIFICATION VARIANCE
SD
SEM
C. V, %
-------
1 control 0.1126 0.3355 0.1678 5,5779
2 5.53 mg/L 0.2849 0.5337 0.2669 8.7353
3 11.3 mg/L 0.1396 0.3737 0.1868 5.7642
4 22.5 mg/L 0.2070 0.4550 0.2275 7.3739
5 49.2 mg/L 2.0769 1.4411 0.7206 48.8520
Title: Nitrite FHM 32-day life stage growth sept 2009
File: N02FHG32. Transform:
ANOVA Table
NO TRANSFORMATION
SOURCE DF
Between 4
Within (Error) 15
Total 19
SS
34.1747
8 . 4628
42 . 6375
MS F
8.5437 15.1434
0.5642
(p-value = 0.0000)
Critical F = 4.8932 (alpha = 0.01, df = 4,15)
= 3.0556 (alpha = 0.05, df = 4,15)
Since F > Critical F REJECT Ho: All equal (alpha = 0.05)
Title: Nitrite FHM 32-day life stage growth sept 2009
File: N02FHG32. Transform:
NO TRANSFORMATION
Dunnett's Test
GROUP
1
2
3
4
5
IDENTIFICATION
control
5.53 mg/L
11.3 mg/L
22.5 mg/L
49.2 mg/L
TABLE 1 OF 2
TRANSFORMED
MEAN
6.0150
6.1100
6.4825
6,1700
2.9500
Ho:Control
-------
1 control 4
2 5.53 mg/L 4 1.2535 20.8 -0.0950
3 11.3 mg/L 4 1.2535 20.8 -0.4675
4 22.5 mg/L 4 1.2535 20.8 -0.1550
5 49.2 mg/L 4 1.2535 20.8 3.0650
-------
nc2 32 day fhm embryo larval mortality LC 25, 20, 10
6
3
3
3 :
3
3
3
control
80
8 '4
0
1.7 mg/L
80
9
1.7
3.4 mg/L
80
11
3 . 4
6.8 mg/L
80
17
6.8
15.0 mg/1
SO
49
15 . 0
3 0 mg/L
80
80
30.0
-------
Title: no2 32 day fhm embryo larval mortality LC 25, 20, 10
File: N02FHMLC. Transform: NO TRANSFORMATION
Probit Analysis - not Using Smoothed Proportions
NUMBER NUMBER OBSERVED PREDICTED
DOSE SUBJECTS OBSERVED PROPORTION PROPORTION
1.70 80 9 0.1125 0,0878
3.40 80 11 0.1375 0.1281
6.80 80 17 ... 0.2125 0.2427
15.00 80 49 0.6125 0.6401
30.00 80 80 1.0000 0.9890
Est. Mu = 12.2157 Est. Sigma = 7 .7629
sd = 0.7102 sd = 0.7337
Chi~Square lack of fit = 2.2245 Likelihood lack of fit = 3.0656
Table Chi-square = 11.3449 (alpha = 0.01, df = 3}
Table Chi-square = 7.8147 (alpha =0.05, df = 3)
Title: no2 32 day fhm embryo larval mortality LC 25, 20, 10
File: N02FHMLC. Transform: NO TRANSFORMATION
Probit LC Estimates
WITHOUT CONTROL DATA
POINT EST. END POINT 95% CONFIDENCE LIMITS
LC 1
-5 . 8435
-8.7922
-2.8948
LC 5
-0.5531
-2 .6501
1.5439
LC10
2 .2672
0.5668
3.9675
LC20
5.6823
4.3413
7.0233
LC25
6.9797
5.7176
8.2418
LC3 0
8.1448
6.9173
9.3724
LC40
10.2490
8.9891
11.5089
LC50
12 .2157
10.8237
13.6077
LC60 14.1824 12.5846 15.7802
LC70 16.2866 14.4143 18.1589
LC75 17.4517 15.4112 19.4922
LC80 18.7491 16.5117 20.9865
LC90 22.1643 19.3757 24.9528
LC95 24.9845 21.7186 28.2505
LC99 30.2749 26.0843 34.4655
-------
MED Toxic Response Analysis Model 10/21/2010 12:44
Log(Exposure Variable)
Parameter Summary (Logistic Equation Regression Analysis)
Parameter
Guess
FinalEst
StdError
95%LCL
95% ue L
LogX50
1.1547
1.1648
0.0276
1,1051
1,2245
S
1,3889
2.824
2,780
-3.182
8.831
Y0
6.311
6.299
0.293
5.667
6.932
Effect Concentration Summary
% Effect
Xp Est
95%LCL
95%UCL
50.0
14.615
12.738
16.770
20.0
11.018
5.574
21,779
10.0
9.339
3,336
26.145
25,0
11.683
6.680
20.434
10/21/2010 12:44 MED Toxic Response Analysis Model, Version 1,03
-------
MED Toxic Response Analysis Model 10/21/2010 12:44
Regression Analysis of Variance
Source
df
SS
MS
F
Alpha
Total
15
41.24
2,749
Regression
2
32,83
16,417
25.4
0,0000
Error
13
8,41
0.647
Data Summary
Exposure
Obs Effects
Pred Effects
Residual
Weight
0.2304
6,8590
6.2993
-0.5597
1.
0.2304
6.1230
6.2993
0.1763
1.
0.2304
5.7660
6.2993
0.5333
1.
0,2304
5.6860
6.2993
0.6133
1.
0,5315
7.0430
6.2945
-0.7485
1.
0,5315
6.3560
6.2945
-0,0615
1,
0.5315
6.2830
6.2945
0.0115
1.
0.5315
6.2610
6.2945
0.0335
1.
0.7782
6.3400
6.2206
-0.1194
1.
0.8325
6.5470
6.1553
-0.3917
1.
0.8325
6,2840
6.1553
-0.1287
1.
0,8325
5,5120
6,1553
0.6433
1.
1.1761
4.2200
2,9492
-1.2708
1.
1.1761
3.4290
2,9492
-0.4798
1.
1.1761
3.2710
2.9492
-0.3218
1.
1,1761
0.8780
2.9492
2,0712
1,
Error Summary
Large Standard Error for Steepness
10/21/2010 12:44
MED Toxic Response Analysis Model, Version 1,03
-------
Appendix 16: Raw Data for 48-hour Toxicity of
Nitrite on Ceriodaphnia dubia
-------
Raw Laboratory Data
-------
TEST MATERIAL:
N.Wv^
INCUBATOR NUMBER;
48-HOUR STATIC ACUTE TOXICITY TEST
DILUTION WATER:
PROJECT NUMBER:_
TEST SPECIES:
TYPE OF TEST:
6jtZ- it.
tdXkiJiCK
NUMBER OF ORGANISMS/CHAMBER:.
NUMBER OF CHAMBERS: S
AGE OF ORGANISMS: \t\
1U75
GLC AND/OR BATCH NUMBER:
TEST TEMPERATURE:_ •t' i "(L
LIGHT INTENSITY (10-20 |ie/M2/S|:. PHOTOPERIOD: 16:8
% z>-
DATE
TEST
DAY
TECH.
INITIALS
TREATMENT LEVEL
ni
CONTROL
f\ i-
g,5,H^L
£ 2Sm5k~
TIME
REPLICATE NUMBER
1
2
3 4
1
2
3
4
1 2 3
4
1
2
3
4
1
2
3
4
1
2
3
4
1 2
3
4
Q
f®
TEMPERATURE fCJ
StS.-O
9^ O
O
pH
R.io
«a\
A, ll
ft, ft
8,m
DO (mg/L)
"1 Q
7 a
7-S
£.0
R.o
e.o
SP. CONDUCTANCE
(/anhosftm)
m
2i^
9-0-1
#S7
1
NUMBER LIVE
<>
S""
S
5
s*
s
s
s
S
5
s
4
3
I
\
I
\
OBSERVATIONS
TEMPERATURE ("C)
3M/1
OW
pH
•?m0
%
T'34-
T-P>
DO (mgiL)
%Y
K-*?
4/0
SP. CONDUCTANCE
(^imhosteiri)
y*-
y
- ^
2
J
NUMBER LIVE
5
b
5
$
s
S
s
5
6
s
5
s
5
5
5
s
a
3
3»
P
P
OBSERVATIONS
TEMPERATURE (*C)
mi
9H1
X
3*?
pH
r m
t-ao
TC'&lo
DO imgIL)
-------
Analytical Chemistry Data
-------
Nitrite Day 0 Ceriodaphnia 48-hour Analytical Results 8.21.09
LabSamplelD
SampteDescription
ParamID
Results Units
RL LabQualifier
AnalysisMethod
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration Person
HB08210324AR Mod Hard water (DMW) control Nitfi N03
1.364
mg/L
0.08854 HT
A4500F
14/10/2009
21/08/2006 Ben Cook
Ben Cook
HB08210324BR Mod Hard water (DMW) control Nitri N03
1.350
mg/L
0.08854 HT
A4500F
14/10/2009
21/08/2009 Ben Cook
Ben Cook
HB08210324A
Mod Hard water (DMW) control Nitri N02
<0.0036 mg/L
0.016425 U
A4500B
21/08/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
HB08210324B
Mod Hard water (DMW) control Nitri N02
<0.0036 mg/L
0.016425 U
A4500B
21/03/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
HB08210329
Water 25mg/L Nitrite-Day 0
N02
25.0
mg/L
3.285
A450Q8
21/08/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
H806210329
Water 25mg/L Nitrite-Day 0
N03
<0.5
mg/L
2.2135 U
A45Q0F
21/08/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
H808210330
Water 3.2mg/L Nitrite-Day 0
N02
3.25
mg/L
0.48275
A4500B
21/08/2009
21/08/2009 Ben COOk
8/21/2009
Ben Cook
HB08210330R
Water 3.2mg/L Nitrite-Day 0
N03
0.53
mg/L
0.22135 HT
A4500F
14/10/2009
21/08/2009 Ben COOk
Ben Cook
HB0821Q331
Water 5.4mg/L Nitrite-Day 0
N02
5.64
mg/L
0.82125
A4500B
21/08/2009
21/08/2009 Ben COOk
8/21/2009
Ben Cook
HB08210331
Water 5.4mg/L Nitrite-Day 0
N03
1.17
mg/L
0.68405
A4500F
21/08/2009
21/08/2009 Ben COOk
8/21/2009
Ben Cook
HB08210332
Water 9.0mg/L Nitrite-Day 0
N02
9.8
mg/L
1.6425
A4500B
21/08/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
HB08210332R
Water 9 Omg/L Nitrite-Day 0
N03
0.2
mg/L
0.66405 J, HT
A4500F
14/10/2009
21/08/2009 Ben Cook
Ben Cook
HB08210333
Water 15,0mg/L Nilrite-Day 0
N02
15,3
mg/L
3.285
A4500B
21/06/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
HB08210333
Water 15.0mg/L Nitrite-Day 0
N03
<,8
mg/L
3.32025 U
A4500F
21/08/2009
21/08/2009 Ben Cook
8/21/2009
Ben Cook
HB08190312
Stock solution 1 5g/L NaN02-1.Qg/l
N03
<27
mg/L
110.675 U
A4500F
21/08/2009
19/08/2009 Ben Cook
8/21/2009
Ben Cook
HB08190312
Stock solution 1.5g/L NaNO2-1.0g/L N02
1140
mg/L
328.5
A4500B
19/08/2009
19/08/2009 Jl
8/19/2009
Jl
W Sa*V'J ^ '~h>l vM""
yet
Page 1
-------
Nitrite Day 2 Ceriodaphnia 48-hour Analytical Results 8,23.09
LabSamptelD
SampleDescription
ParamID
Results Units
RL LabQualifier
Ana lysis Met hod
AnalysisDate
SampleDate Analyst
Calibration Date
Calibration
HB08230343R
Water 3.21T13/L Nitrite-Day 2
N03
1.46 '
mg/L
0.22135 HT
A4500F
14/10/2009
23/08/2009 Ben Cook
Ben Cook
HB0823Q343
Water 3.2mg/L Nitrite-Day 2
N02
3.23
mg/L
0.82125
A4500B
24/08/2009
23/08/2009 Jl
8/24/2009
Jl
HB08230344AR Water 5,4mg/L Nitrite-Day 2
N03
1.46
mg/L
0.4427 HT
A4500F
14/10/2009
23/08/2009 Ben Cook
Ben Cook
HB0823Q344BR Water 5.4mg/L Nitrite-Day 2
N03
1.37
mg/L
0.4427 HT
A4500F
14/10/2009
23/08/2009 Ben Cook
Ben Cook
HB08230344A
Water 5.4mg/L Nitrite-Day 2
N02
5.0
mg/L
1.6425
A4500B
24/08/2009
23/08/2009 Jl
8/24/2009
Jl
HB08230344B
Water 5.4mg/L Nitrite-Day 2
N02
5.4
mg/L
1.6425
A4500B
24/08/2009
23/08/2009 JI
8/24/2009
Jl
HB08230345
Water 9,0mg/L Nitrite-Day 2
N03
1.66
mg/L
0.8854
A4500F
24/08/2009
23/08/2009 Ben Cook
8/24/2009
Ben Cook
HB08230345
Water 9.0mg/L Nitrite-Day 2
N02
8.6
mg/L
1.6425
A4500B
24/08/2009
23/08/2009 JI
8/24/2009
Jl
HB08230346R
Water I5,0mg/L Nitrite-Day 2
N03
1.8
mg/L
0.8854 HT
A4500F
14/10/2009
23/08/2009 Ben Cook
Ben Cook
HB08230346
Water 15,Omg/L Nitrite-Day 2
N02
15,5
mg/L
3.285
A45008
24/082009
23/08/2009 JI
8/24/2009
J!
HBQ823Q347
Water 25,Omg/l Nitrite-Day 2
N03
<0.5
mg/L
2.2135 U
A450QF
24/08/2009
23/08/2009 Ben Cook
8/24/2009
Ben Cook
HB0823Q347
Water 25.0mg/L Nitrite-Day 2
N02
23.0
mg/L
4,10625
A4500B
24/08/2009
23/08/2009 Jl
8/24/2009
Jl
HB08230348
Water control Nitrite-Day 2
N03
0.773
mg/L
0.08854
A4500F
24/08/2009
23/08/2009 Ben Cook
8/24/2009
Ben Cook
HBQ8230348
Water control Nitrite-Day 2
N02
0.0651
mg/L
0.016425
A4500B
24/08/2009
23/08/2009 Jl
8/24/2009
Jl
zxce^det. Sa^Ux Uiire d^e.
$vfpxrci !*;+<*( wfvff,
XBc
Page 1
-------
DILUTE MINERAL WATER (DMW)
PREPARATION DATA FORM
Great Lakes Environmental Center
BATCH NUMBER: /h*?y TOTAL VOLUME:_ tit
DATE PREPARED: B/m/O9 PREPARED BY: Jtfi
DEtONlZED WATER SOURCE:
MINERAL WATER SOURCE: r
VOLUME OF BASE WATER (mL);
VOLUME OF MINERAL WATER fmL): fbOO
DMW WATER QUALITY
DATE CHECKED: CHECKED BY: AkJ
PH:
Specific conductivity («mhqs/cm): \ TU
HARDNESS (rng/L as CACO,1;
ALKALINITY (mg/L as CACO,): "lb
Hardness: Alkalinity; „
End mis: ob'i End mis: | ¦
Start mis: Start mis: TvF
-------
PROJECT#: 6112-12 Date 21/08/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 0
CALIBRATION
Concentrations i
ppm)
Natural log
Millivolts
0.5
-0.6931472
-5.0
1
0
-26.6
10
2.30258509
-38.4
25
3.21887582
-95.7
50
3.91202301
-118.9
-136.8
Regression Output:
Constant
-17.929814
Std Err of Y Est
20.489165
R Squared
0.864628
No. of Observations
5
Degrees of Freedom
3
X Coefficients) -22.304739
Std Err of Coef. 5.09549264
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
C.dubia
• Con
0
water
4
-0.983191
0.37411548
-0.185957
C.dubia
25mg/Lno2
0
water
2
-0.893524
0.40921126
-0.1508612
0
water
-0.803857
0.44759938
-0.1124731
n/a
Blank
0
n/a
-5
-0.579689
0.56007248
0
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
£:WVC
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax:231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mai
Page
ofL
of
'Jamie Saxton
fVv C\^riro-^
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below'
Alkalinity
c
O
«
r'S
Chlorophyll a
• Hardness
Z
m
s
z
It
rs
1
en. ¦:
O
%
TDS
PU
H
m - ¦
m-.
H .
Nitrite
NO2dub48W-0-l
8/21/09
125 ml
HDPE
Water 3.2 mg/L Nitrite -
DayO
h2so4
x':
NO2dub48W-0-2
8/21/09
125 mL
HDPE
Water 3.2 mg/L Nitrite -
DayO
Cold
-I;,- •.
'y - :¦
X
NO2dub48W-0-3
8/21/09
125 mL
HDPE
Water 5.4 mg/L Nitrite -
DayO
h2so4
\:V-
• - i * . _•
X
NO2dub48W-0-4
8/21/09
125 mL
HDPE
Water 5.4 mg/L Nitrite -
DayO
Cold
A*,--
Mi
' : '
X
NO2dub48W-0-5
8/21/09
125 mL
HDPE
Water 9.0 mg/L Nitrite -
DayO
H2S04
.( ' •
:V;:
.O:;*
X
NO2dub48W-0-6
8/21/09
125 mL
HDPE
Water 9.0 mg/L Nitrite -
Day 0
Cold
.......
X
Shipper:
Date/Time:
Receiver:
Date/Time:
MM
Temperature:
BOD, = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj -N = Ammonia-Nitrogen TKM = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NQ3/NO2-N =NiWe/Nitrite-Nitrogen
N02-N = Nitrite Nitrogen
NOj -N = Nitrate Nitrogen
Total N - Total Nitrogen
SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
<2 of">
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailec Garton/Jamie Saxton
/V-
Bottle ID
Date I Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity:
a
o
S3
Chloride
Chlorophyll a
; Hardness
z
rt
X
Z
: '5:
m
©
. z
TDS
H
ft-
H
m
ifi
H
• *«*"¦;'
• (BUT.
.
JfL
4=
NO2duh48W-0-7
8/21/09
125 mL
HOPE
Water 15.Gmg/L Nitrite -
Day 0
h2so4
¦¦ ¦€'-1
X .
NO2dub48W-0-8
8/21/09
125 mL
HDPE
Water 15.0mg/L Nitrite -
Day 0
Cold
X
X
•
|§f§
¦
X
J
X;-;v
X
Shipper:
Date/Time:
Receiver:
iti
Date/Time:
$¦-21 -o^
Temperature:
2.
BODs = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NOj/NO; -N - Nitrate/Nitrife-Nitrogcn
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS — Total Suspended Solids
Abbreviations for other frequently requested analyses
NOi-N = Nitrite Nitrogen Total N = Total Nitrogen
N03 -N = Nitrate Nitrogen SRP - Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
3 „f 3
GjjC
Phone:231-941-2230
Fax:231-941-2240
www.glcc.com
Great Lakes Environmental Center
Facility:
, . Traverse City, MI
Location;
Date;
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Al GtW
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
citccK me i ci|UOicu aiiaijaia uciuiiuuua uciun
P
r S'ht ¦
,,.. .
' ^^3' •
m
Q
O
<» : -
• '-2'
Chlorophyll a
Hardness
z
1
S
Z
Z
(St
0
&
7- 55
OD
Q
H
;
su
H
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
f t
vjLBw
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.clec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, MI
Location:
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below1
: -s"' r
*«-'
^ '
¦<
«#>
a
0
I:
Chlorophyll a
Hardness
Z
(
EC
Z
z ;
1
7-
rt --
O
z -
(Z>
ft
1-
'•pit
Cu
V)
m-
H
Nitrate
Nitrite
NO2dub48W-0-1
Z-
8/23/09
125 mL
HOPE
Water 3.2 mg/L "Nitrite -
Day 2
h2so4
v -
X
N02dub48W-|-2
8/23/09
125 mL
HOPE
Water 3.2 mg/L Nitrite -
Day 2
Cold
:
X
N02dub48W-jP-3
I
8/23/09
125 mL
HOPE
Water 5.4 mg/L Nitrite -
Day 2
h2so<
X
N02dub48W-/f-4
t
8/23/09
125 mL
HOPE
Water 5.4 mg/L Nitrite -
Day 2
Cold
X
N02dub48W-f5
8/23/09
125 mL
HDPE
Water 9.0 mg/L Nitrite -
Day 2
h2so4
V
....
X
N02dub48W-2 -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS= Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
Date/Time:
Temperature:
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NO- -N - Nitrite Nitrogen Total N - Total "Nitrogen
NOj -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
jrLiX-
Page
^ ' .y
'"'739 Hastings St"
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glee.com
Great Lakes Environmental Center
Facility:
, Traverse City, Ml
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
¥ I'OH
Mailee Garton/Jamie Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definition!) below
' 3i
BODs
Chloride:
Chlorophyll a
z
po
a
z
m-:om/€o\t
1
•H'
a.
H
co
w
H
©
¦m. ¦
at..
. 1m ¦
IZh
4?
c
—
"2
N02dub48W-^-7
L
8/23/09
125 mL
HDPE
Water 15.Omg/L Nitrite -
Day 2
h2so4
x .•
N02dub48W-/J-8
1
8/23/09
125 mL
HOPE
Water 15.Omg/L Nitrite -
Day 2
Cold
X
NQ2dub48W-0-9
¦L
8/23/09
125 mL
HDPE
Water 25.0mg/L Nitrite -
Day 2
H2SO4
X :
N02dub48W-«f-
10 i
8/23/09
125 mL
HDPE
Water 25.0mg/L Nitrite -
Day 2
Cold
v-'s
X
N02dub48W-j^-
11 -
8/23/09
125 mL
HDPE
Water control Nitrite-
Day 2
h2so4
<•.' "
x
N02dub48W-f
12 1
8/23/09
125 mL
HDPE
Water control Nitrite -
Day 2
Cold
/v' "
X
Shipper:
Date/Time:
Receiver:
tLn (/!
Date/Time:
t-Z<1 of
Temperature:
Definitions
8OD5 = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NHj -N = Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS= Total Suspended Solids
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen
Abbreviations for other frequently, requested analyses
N02 -N = Nitrite Nitrogen Total N = Total Nitrogen
NOs -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
Reference Toxicant Data
-------
( jT p( Ceriodaphnia dubia Survival
Sodium Chloride (NaCl) Toxicity Data
2007-2009
3.5
3
2.5
C3
tJD
© 2
in
O
^ 1.5
it
-------
Statistical Analyses
-------
EPA PROBIT ANALYSIS PROGRAM
USED FOR CALCULATING LC/EC VALUES
Version 1.5
NITRITE C. DUBIA 48 HR {24
Proportion
Chi
Chi
Observed
Responding
Predicted
Number
Number
Proportion
Adjusted for
Proportion
Cone.
Exposed
Resp,
Responding
Controls
Responding
3 , 2400
20
0
0.0000
¦ 0.0000
0.0000
5 .3500
20
0
0 .0000
0.0000
0.0000
9.2000
20
0
0.0000
0.0000
0.0042
15.5000
20
5
0.2500
0.2500
0.2339
24.0000
20
16
0.8000
0.8000
0.8085
- Square
for Heterogeneity
(calculated)
0.123
- Square
for Heterogeneity
(tabular value
at 0.05
level)
7.815
Mu
Sigma
Parameter
1. 276575
0.118801
Estimate
Std, Err.
95% Confidence Limits
Intercept
Slope
-5.745512
8.417452
2.536098
1.989686
-10.716264,
4.517663,
Theoretical Spontaneous Response Rate
0.0000
-0.774759)
12.317241)
NITRITE C. DUBIA 4 8 HR (24
Estimated LC/EC Values and Confidence Limits
Exposure 95% Confidence Limits
Point
Cone.
Lower
Upper
LC/EC
1.00
10.005
5.719
12 .
465
LC/EC
5 . 00
12.055
8 . 022
14
286
LC/EC
10. 00
13.314
9.576
15 .
414
LC/EC
15 . 00
14 .238
10 .765
16.
265
LC/EC
50.00
18.905 ^
16.62 3 .-
21.
683
LC/EC
85.00
25.102
21.846
33 .
963
LC/EC
90.00
26 . 843
23 . 027
38 ,
223
LC/EC
95.00
29.648
24.821
45 .
672
LC/1C
99.00
35 . 722
28.423
64 .
115
NITRITE C. DUBIA 4 8 HR (24
-------
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE
Probit
10 +
9+
7 +
6+
. . . o
5 +
4 +
3 +
2 +
1 +
O+o
- -j— - — - . - — — .j - - .j. _ _j. — — — 4...
ECO1 EC10 EC25 EC50 EC75 EC90 EC99
-------
TRIMMED SPEARMAN-KARBER METHOD. MONTANA STATE UNIV
FOR REFERENCE, CITE;
HAMILTON, M.A., R.C, RUSSO, AMD R.V. THURSTON, 1977.
TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING MEDIAN
LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS.
ENVIRON. SCI. TECHNOL. 11(7); 714-719;
CORRECTION 12 (4) :417 (1978) .
DATE; 8/21/09 TEST NUMBER: 6112-12
CHEMICAL: SODIUM NITRITE SPECIES: C.DUBIA
DURATION (HOURS ) LC50 LOWER 95% LIMIT UPPER 95% LIMIT PERCENT
TRIM
RAW DATA:
CONCENTRATION(MG/L
NUMBER EXPOSED;
3.24 5.35 9.20 15.50 24.00
20 20 20 20 20
20 . 00
48
24
15 .18
18 .86
13 . 63
15 . 15
23.46 4br2Hlrvn
16.89
00
-------
Appendix 17: Raw Data for 7-day Toxicity of
Nitrite on Ceriodaphnia dubia
-------
Raw Laboratory Data
-------
DAPHIN1D 7-DAY STATIC RENEWAL CHRONIC TOXICITY TEST
Page
I oP
TEST MATERIAL:
TYPE OF TEST
PROJECT NUMBER .
TEST SPECIES: C. Jubm
bWL- i7-
DILUTION WATER: .
INCUBATOR NUMBER:,
BP
jfca
JpfW IblS
PHOTOPERIODIC):.
TEST TEMPERATURE:IMC
LIGHT fNTENSITY < JO-20 V Dav 0/
DILUTION WATER BATCH NUMBER:
DAY Q/V
vVfe DAY^JaM DAY /
GLC NUMBER: DAY ft I
FOOD BATCH NUMBER.: DAY 0.
YOUNG-AGE/SOU RCE: < 24 hours in lipase
,n% day *7 / f*fi
mo.
NOTE; D.O. & pH measured «
Temperature mcisored in all atn
' Specific Conductance
em. merfbin. and high concentrations in new snd old solutions,
solutions £ two randomly selected old solutions.
Key: += alive? --dead; e « efjs present;
P = Pale: F= Floating; SB - Stuck to bubble
y, E fry,1t-
-------
i J. ,i
TEST MATERIAL:.
N
PROJECT NUMBERl
TEST SPECIES: C. dublo
TYPEOFTESTl
INCUBATOR. NUMBER
DAPHNID 7 DAY STATIC RENEWAL CHRONIC TOXICITY TEST
W^aa)
Page
fY|7Z{iii m r&zo*
^ ^ AJaA/ Ajjit 1 r^ulf
DAY
r
DAY
8 '
/lUlr
mor
CONCENTRATIONS
TEST CHAMBER NUMBER
PH
OLD
DISSOLVED QXVCEN
TEMPERATURE
OLD
sc"
NEW
C
(5,25^
r~ \''0
y '
4-
4jl.
jfc_
j/ i'
±
V
^jlTZ
4Sb
40
LiS
g.o
£
a*
A
4i_
IcktD
$3
EH
M-
cjtt-O
-£f
i-
etn_y
laa.
IS
Si
$—*CL
fC
dip
£2lL
is
SO.
TO
%2
°W>
r.9?
fcfcLalLii
37iO
1c7
ogH
if?-?/
try-' r
ck.Cg
tr'6?
35?
^,2-
.£?
A-
sky_
4^
4t ft
jj~€_
4- |
¦fi-"
^2i
Eiii
W
r-SH
4" I
S-Q
mo_
7&>o
m
j-c-
okS
tf~-
^ 3
Ei
-7ZT
ew solutions & rwo randomly selected aid soliitions.
Spectfic CmdudiiKt
Key: + - alive; - - dead;
P= Pale; F= Floating; SB =
; ™ eggs pfesenl;
Stuck to bubble
-------
U.l
DAPHNIL) 7-DAY STATIC RENEWAL CHRONIC TOXICITY TEST
TEST MATERIAL:
PROJECT NUMBER.
TEST SPECIES: C,
K^VuJU
TYPE OF TEST:„
rNCUBATOR NUMBER:
j/wJGlM
DILUTION WATER:
Of
fed.
PHOTOPERJOD(LD).
TEST TEMPERATURErJidlC.
LIGHT INTENSITY (10-20 ^E/mVs >. DavO/
DILUTION WATER BATCH NUMBER:
DAY M DAY / DAY /
GLC NUMBER: DAY
TECHNICIANS INITIALS:
rSM1 rfvi/tff9' sywf
DAY
?
CONCENTRATIONS
TEST CHAMBER NUMBER
PH
OLD
DISSOLVED OXYGEN
NEW
TEMPERATURE
t^
or
4
fgT"
_ki
«Ub
§ iiL
-sfc
¦M
A
e¥f£
-t-
_k_
V"
4g>
v
4-
gji
A=-
10.
Cf\
tm
25.0
w
j~*L.
xM-
dt-fo
5&G
H
€C>1
•--¦il-mJEmm
ea Q1
25.0
25,0
NOTE: D.O. & pH measured in low> medktm, and high concentrations in new and old sohrtHwi.
Tempernlurc measured in ill new solutions & two randomK selected old solutions.
"Specific Conductance
Key: +° alive: - - dead; #«= eggs present;
P - Pale; F= Floating; SB » Stuck to bubble
-------
Young Summary
Test Material/Client: Nitrite HECD B-12 Technician's Intitals MWG
Project Number: 6112-12 QC InititalsL^
Test Dates; 8/21/09-8/27/09
GLC Numbers: N/A
Nominal (and Measured) Test Concentrations
-------
Analytical Chemistry Data
-------
GleC
DILUTE MINERAL WATER (DMW)
PREPARATION DATA FORM
Great Lakes Environmental Center
BATCH NUMBER;,
DATE PREPARED:
S.-2M
TOTAL VOLUME:.
PREPARED BY:
.
(V\u)tr-
DEIONIZED WATER SOURCE:
MINERAL WATER SOURCE:
^AXxa/>
VOLUME OF BASE WATER (mL):_
VOLUME OF MINERAL WATER (mL):
L*oQi—-I
DMW WATER QUALITY
DATE CHECKED:.
PH: SJh :
Specific conductivity (^mhos/cm):_
HARDNESS (mg/L as CACQ,1:
ALKALINITY (mg/L as CAC0,1: *7%
CHECKED BY:
6K
Hardness:
End mis: )j< 7
Start mis; 9S
Alkalinity:
End mis; m.f
Start mis; ~7. Z-
-------
PROJECT#: 6112-12 Date 21/08/2009
PROJECT NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 0
CALIBRATION
Concentrations
ppm)
Natural Log
Millivolts
0.5
-0.6931472
-5.0
1
0
-26.6
10
2.30258509
-38.4
25
3.21887582
-95.7
50
3.91202301
-118.9
-136,8
Regression Output:
Constant
-17.929814
Std Err of Y Est
20.489165
R Squared
0.864628
No. of Observations
5
Degrees of Freedom
3
X Coefficient^) -22.304739
Std Err of Coef. 5.09549264
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
C.dubia
Con
0
water
4
-0.983191
0.37411548
-0.185957
C.dubia
25mg/Lno2
0
water
2
-0.893524
0.40921126
-0.1508612
0
water
-0.803857
0.44759938
-0.1124731
n/a
Blank
0
n/a
-5
-0.579689
0.56007248
0
-------
PROJECT#; 6112-12 Date 27/08/2009
PROJECT "NAME HECD B-12 Nitrate, Nitrite, Boron, Flouride
Test Day 6
CALIBRATION
Concentrations i
ppm)
Natural log
Millivolts
0.5
-0.6931472
-24.2
1
0
-35.9
10
2.30258509
-94.1
25
3.21887582
-117.8
50
3.91202301
-131.7
Regression Output:
Constant
-38.726752
Std Err of Y Est
2.349771
R Squared
0.998230
No. of Observations
5
Degrees of Freedom
3
X Coefficients) -24.034113
Std Err of Coef. 0.58436929
OUTPUT
Predicted
Ammonia
Test
GLC
Test
Test
Milli-
Natural
Ammonia
Minus
Organism
Number
Day
Material
volts
Log
Levels
Blank
C.dubia
Con
6
water
-1
-1.569717
0.2081041
-0.0970533
C.dubia
12,5mg/Lno2
6
water
-1.2
-1.561395
0.20984306
-0.0953143
6
water
-1.611324
0.19962306
-0.1055343
n/a
Blank
6
n/a
-10.2
-1.186928
0.30515739
0
-------
Nitrite Day 0 Ceriodaphnia 7-day chronic Analytical Results 8.21.09
AO/life*
LabSampIelD
SampleDescripfion
ParamlD
Results
Units
RL
LabQualifier
AnalysisMethod
AnaiysisDate
SampfeDate
Analyst
Calibration Date
HB08210324AR Mod Hani water (DMW) control
Nitri
N03
1.364
mg/L
0.08854
HT
A4500F
14/10/2008
21/08/2009
Ben Cook
HB08210324BR
Mod Hard water (DMW) contra!
Nitri
N03
1.350
mg/L
0.08854
HT
A4500F
14/10/2009
21/08/2009
Ben Cook
HB08210324A
Mod Hard water (DMW) control
Nitri
N02
<0.0036 mg/L
0.016425
U
A4500B
21/08/2009
21/08/2009
Ben Cook
8/21/2009
HB08210324B
Mod Hard water (DMW) control
Nitri
N02
<0.0036
mg/L
0.016425
U
A45008
21/08/2009
21/08/2009
Ben Cook
8/21/2009
HB08210325
Water 1.57mg/L Nitrite-Day 0
N02
1.63
mg/L
0.16425
A4500B
21/08/2009
21/08/2009
Ben Cook
S/21/2009
HB08210325R
Water 1 _S7nug/L Nitrite-Day 0
N03
0.49
mg/L
0.17708
HT
A4500F
14/10/2009
21/08/2009
Ben Cook
HB08210326
Water 3.13mg/t Nitrite-Day 0
N02
3.28
mg/L
0.49275
A4500B
21/08/2009
21/08/2009
Ben Cook
8/21/2009
HB08210326R
Water 3 13mg/L Nitrite-Day 0
N03
1.06
mg/L
0.22135
HT
A4500F
14/10/2009
21/08/2009
Ben Cook
HB08210327
Water 6.25m 9/1 Nitrite-Day 0
N02
6.36
mg/L
0.82125
A4500B
21/08/2009
21/08/2009
Ben Cook
8/21/2009
HB08210327
Water 6.25mg/L Nitrite-Day 0
N03
0.67
mg/L
0.4427
A4500F
21/08/2009
21/08/2009
Ben Cook
8/21/2009
HB08210328
Water 12.5mg/L Nitrite-Day 0
N02
12.4
mg/L
1.8425
A4500B
21/08/2009
21/08/2009
Ben Cook
8/21/2009
HB08210328
Water I2,5mg/L Nitrite-Day 0
N03
1,23
mg/L
0.8854
A4500F
21/08/2009
21/08/2009
Ben Cook
8/21/2009
HB08210329
Water 25mg/L Nitrite-Day 0
NC2
25.0
mg/L
3.285
A4500B
21/08/2009
21/08/2000
Ben Cook
8/21/2009
HB0B210329
Water 25mg/L Nitrite-Day 0
N03
<0.5
mg/L
2.2135
U
A4500F
21/08/2009
21/08/2009
Ben Cook
8/21/2009
HB08190312
Stock solution 1.5g/L NaN02-1.
•Og/L
N03
<27
mg/L
110.675
U
A4500F
21/08/2009
19/08/2009
Ben Cook
8/21/2009
HB08190312
Stock solution 1.5g/L NaNO2-1.0g/L
N02
1140
mg/L
328.5
A4500B
19/08/2009
19/08/2009
Jl
8/19/2009
Calibration Person
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Ben Cook
Jl
i/v
Page 1
-------
Nitrite Day 1 Cenodaphnia 7-day chronic Analytical Results 8.22.09
LabSamplelD
SampleOescription ParamlD
Results
Units
RL
LabQualifier
AnalysisMethod
Analysis Date
SampleDate
Analyst Calibration Date
Calibration Person
H808220334A
Mod Hand water(DMW) control Nitrit N03
<0.0053 mg/L
0.022135
U
A450OF
24/08/2009
22/08/2009
Ben
Cook 8/24/2009
Ben
Cook
HB08220334A
Mod Hard water(DMW) control Nitrit N02
D.0200
mg/L
0,016425
A4500B
24/08/2003
22/08/2009
Jl
8/24/2009
Jl
HB08220334B
Mod Ha(d water(DMW) control Nitrit N03
<0,0053 mg/L
0.022135
U
A4500F
24/08/2009
22/08/2009
Ben
Cook 8/24/2009
Ben
Cook
HB08220334B
Mod Hard water(DMW) control Nitrit N02
0.0164
mg/L
0.016425
J
A4500B
24/08/2009
22/08/2009
Jl
8/24/2009
Jl
HB08220335
Water 1,57mg/L Nitrite-Day1 old sol N03
0.815
mg/L
0.17708
A4500F
24/08/2009
22/08/2009
Ben
Cook 8/24/2009
Ben
Cook
HB08220335
Water 1,57mg/L Nitrite-Oay1 old sol N02
1.11
mg/L
0.3285
A45O0B
24/08/2009
22/08/2009
Jl
8/24/2009
Jl
HB08220338
Water 3,13mg/L Nitrite-Day1 old sol N03
1.13
mg/L
0.22135
A4500F
24/08/2009
22/08/2009
Ben
Cook 8/24/2009
Ben
Cook
HB08220336
Water 3,13mg/L Nitrite-Day1 old sol N02
2.64
mg/L
0,82128
A4500B
24/08/2009
22/08/2009
Jl
8/24/2009
Jl
HB08220337
Water 6,25mg/L Nitrite-Day1 old sol N03
1.03
mg/L
0.4427
A4500F
24/08/2009
22/08/2009
Ben
Cook 8/24/2009
Ben
Cook
H808220337
Water 6,25mg/L Nitrite-Day1 old sol N02
5.3
mg/L
1.6425
A4500B
24/08/2009
22/08/2009
Jl
8/24/2009
Jl
HB08220338
Water 12.5mg/L Nitrite-Day1 old sol M03
0.18
mg/L
0.8854
J
A4500F
24/08/2009
22/08/2009
Ben
Cook 8/24/2009
Ben
Cook
HB08220338
Water 12.5mg/L Nitrite-Dayl old sol N02
12.5
mg/L
3.285
A4500B
24/08/2009
22/08/2009
Jl
8/24/2009
Jl
HB08220339
Water 25mg/L Nitrite-Dayl old solut N03
<0.5
mg/l
2.2135
U
A4500F
24/08/2009
22/08/2009
Ben
COOk 8/24/2009
Ben
Cook
HB08220339
Water 25mg/L Nitrite-Day1 old solut N02
23.0
mg/L
4.10625
A4500B
24/08/2009
22/08/2008
Jl
8/24/2008
Jl
Page 1
-------
Nitrite Day 2 Ceriodaphnla 7-day chronic Analytical Results 8,23.09
LabSampIelD
SampleDescription
ParamID
Results Units
RL LabGualifier
AnalysisMethod
AnalysisDate
SampteDate Analyst Calibration Date
Calibration Person
HB0823034Q
Mod Hard water(DMW) control Nltrit N03
1.142
mg/L
0.08854
A45Q0F
24/08/2009
23/08/2009 Ben Cook 8/24/2009
Ben Cook
HB0823Q340
Mod Hard water(DMW) control Nitrit N02
0.0069
mg/L
0.016425 J
A4500B
24/08/2009
23/08/2009 Jl 8/24/2009
Jl
[HB08230341
Water 1.57mg/t Nitrite-Day 2
N02
1.58
mg/L
0.3285
A4500B
24/08/2009
23/08/2009 Jl 8/24/2009
Jl
J-IB08230341A
Water 1.57mg/l Nitrite-Day 2
N03
1.104
mg/L
0.17708
A4500F
24/08/2009
23/08/2009 Ben Coo* 8/24/2009
Ben Cook
HB08230341B
Water 1.57mg/L Nitrite-Day 2
N03
1.022
mg/L
0.17708
A4500F
24/08/2009
23/08/2009 Ben Cook 8/24/2009
Ben Cook
HB0B230342
Water 6.25mg/L Nitrite-Day 2
N03
1.43
mg/L
0.4427
A4500F
24/08/2009
23/08/2009 Ben Cook 8/24/2009
Ben Cook
HB08230342
Water 6.25mg/L Nitrite-Day 2
N02
6.5
mg/L
1.6425
A4500B
24/08/2009
23/08/2009 Jl 8/24/2009
Jl
Page i
-------
Nitrite Day 3 Ceriodaphnia 7-day chronic Analytical Results 8,24.09
LabSamplelD SampteDescription ParamID
HB082403S3 Water 1,57mg/L Nitrite-Day 3 old so N02
HB082403S3 Water 1 ,57mg/L Nitrite-Day 3 old so N03
HB08240354A Water B.25mg/L Nitrite-Day 3 old so N02
HBQ8240354A Water 6.25mg/L Nitrite-Day 3 old so N03
H808240354B Water 6.25mg/L Nitrite-Day 3 old so N02
HB0824Q354B Water 6.25mg/L Nitrite-Day 3 old so N03
Results Units RL LabQualilier AnalysisMethod AnalysisDate SampleDate Analyst Calibration Date Calibration Person
0.74 mg/L 0.3285 A45D0B 25/08/2009 24/08/2009 Jl 8/25/2009 Jl
0.491 mg/L 0.13281 A4500F 26/08/2009 24/08/2009 Ben Cook 8/26/2009 Ben Cook
5.6 mgII 1.6425 A4500B 25/08/2009 24/08/2000 Jl 8/25/2008 Jl
0,64 mg/L 0.4427 A4S00F 26/08/2009 24/08/2009 Ben Cook 8/26/2009 Ben Cook
5.3 mg/L 1.6425 A4S00B 25/08/2009 24/08/2009 J[ 8/25/2009 J!
0.95 mg/L 0.4427 A4500F 26/08/2009 24/08/2009 Ben Cook 8/26/2009 Ben Cook
Page 1
-------
Nitrite Day 4 Ceriodaphnia 7-day chronic Analytical Results 8,25,09
LabSamplelD
SampleDescription
ParamID
Results Units
RL LabQualifier
AnalysisMethod
AnalysisDate
SampleDate Analyst Calibration Date
Calibration Person
HBQ825Q3S5
Water 3,13mg/L Nitrite-Day 4
N02
2,78
mg/L
0.82125
A4500B
25/08/2009
25/08/2009 Jl 8/25/2009
Jl
HB0825Q355
Water 3.13mg/l Nitrite-Oay 4
N03
1.58
mg/L
0,22135
A4500F
26/08/2009
25/08/2009 Ben Cook 8/26/2009
Ben Cook
HB082503S6
Water I2.5mg/t Nitrite-Day 4
N02
11.9
mg/L
3.285
A4500B
25/08/2009
25/08/2009 Jl 8/25/2009
Jl
HB08250356
Water I2.5mg/l Nitrite-Day 4
N03
2.47
mg/L
0,8854
A4500F
26/08/2009
25/08/2009 Ben Cook 8/26/2009
Ben Cook
Page 1
-------
Nitrite Day 5 Ceriodaphnia 7-day chronic Analytical Results 8.26,09
LabSamplelD
SampleDescription ParamID
Results
Units
RL LabQualifier
AnalysisMethod
AnalysisDate
SampleDate Analyst Calibration Date
Calibration Person
HBQ8260378
Mod Hard (DMW) control Nitrite-Day N02
0.0484
mg/L
0.016425
A4500B
28/08/2009
26/08/2009 J 8/28/2009
Jl
HB08260378A
Mod Hard (DMW) control Nitrite-Dai N03
0.3S1
mg/L
0.08854
A450GF
28/08/2009
26/08/2009 Ben Cook 8/28/2009
Ben Cook
HBQ8260378B
Mod Hard (DMW) control Nitrite-Dai N03
0.328
mg/L
0.08854
A4500F
28/08/2009
26/08/2009 Ben Cook 8/28/2009
Ben Cook
HB08260379
Water 3.13mg/l. Nitrite-Day 5 old so N03
0.78
mg/L
0.26562
A45O0F
28/08/2009
26/08/2009 Ben Cook 8/28/2009
Ben Cook
HB082B0379
Water 3,13mg/L Nitrite-Day 5 old so N02
2.44
mg/L
0.82125
A4500B
28/08/2009
26/08/2009 JI 8/28/2009
Jl
HB08260380
Water 12.5mg/L Nitrite-Day 5 old so N03
3.62
mg/L
0.8854
A4500F
28/08/2009
26/08/2009 Ben Cook 8/28/2009
Ben Cook
HB08260380
Water 12,5mg/L Nitrite-Day 5 old so N02
10.8
mg/L
3.285
A45G0B
28/08/2009
26/08/2009 Jl 8/28/2009
Jl
Page 1
-------
Nitrite Day 6 Ceriodaphnia 7-day chronic Analytical Results 8,27.09
LabSamplelD
SampleDescription ParamID
Results
Units
RL labQualifier
AnalysisMethod
AnaiysisDate
SampleDate Analyst Calibration Date
Calibration Person
HB08270384
Mod Hard (DMW) control Nitrite-Das N02
0.0531
mg/L
0.016425
A4500B
28/08/2009
27/08/2009 Jl 8/28/2009
Jl
HB08270384A
Mod Hard (DMW) control Nitrite-Daj N03
<0.0053 mg/L
0.022135 U
A4500F
28/08/2009
27/08/2009 Ben Cook 8/28/2009
Ben Cook
HB08270384B
Mod Hard (DMW) control Nitrite-Da^ N03
<0.0053 mg/L
0.022135 U
A4500F
28/08/2009
27/08/2009 Ben Cook 8/28/2009
Ben Cook
HB08270385
Water 1.57mg/L Nitrite-Day 6 old so N03
0.703
mg/L
0.17708
A4500F
28/08/2009
27/08/2009 Ben Cook 8/28/2009
Ben Cook
HB08270385
Water 1,57mg/L Nitrite-Day 6 old so N02
0.87
mg/L
0.3285
A4500B
28/08/2009
27/08/2009 Jl 8/28/2009
Jl
HB08270386
Water 3.13mg/L Nitrite-Day 6 old so N03
1.11
mg/L
0.22135
A4500F
28/08/2009
27/08/2009 Ben Cook 8/28/2009
Ben Cook
HB0827Q386
Water 3.i3mg/L Nitrite-Day 6 old so N02
2.35
mg/L
0.82125
A4500B
28/00/2009
27/08/2009 Jl 8/28/2009
Jl
HB08270387
Water 6.25mg/l Nitrite-Day 6 old so N03
0.55
mg/L
0.4427
A4500F
28/08/2009
27/08/2009 Ben Cook 8/28/2009
Ben Cook
HB08270387
Water 6.25m^L Nitrite-Day « old so N02
5.5
mg/L
1.6425
A4500B
28/08/2009
27/08/2009 Jl 8/28/2009
Ji
HB08270388
Water 12.5mg/L Nitrite-Day 6 old so N03
1.47
mg/L
0.8854
A4500F
28/08/2009
27/08/2009 Ben Cook 8/28/2009
Ben Cook
HB08270388
Water 12.5mg/L Nitrite-Day 6 old so N02
11.1
mg/L
3,285
A4500B
28/08/2009
27/08/2009 Jl 8/28/2009
Jl
HB08270389
Stock solution 1.5g/L NaN02-1.0g/L N03
<21
mg/L
88.54 U
A4500F
28/08/2009
27/08/2009 Ben Cook 8/28/2009
Ben Cook
HB0827038S
Stock solution 1.5g/L NaNO2-1.0g/l N02
820
mg/L
328.5
A4500B
28/08/2009
27/08/2009 Jl 8/28/2009
Jl
Page 1
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page J
GieHti-akos
739 Hastings St,
Traverse City, MI 49686
Phone: 231-941-2230
Fax:231-941-2240
www.giec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
9: fa I
Mailee Garton/Jaime Saxton
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information 1
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
O
O
as
Chloride
Chlorophyll a
Hardness |
z.
s
2
Z ¦
TDS
0*
H
H
Nitrate
Nitrite
NO2dub7W-0-1 b
8/21/09
125 mL
HOPE
Mod Hard water (DMW)
control Nitrite-Day 0
h,so4
X
NO2dub7W-0-2b
8/21/09
125 mL
HDPE
Mod Hard water (DMW)
control Nitrite-Day 0
Cold
i-' :
X
1^/ "
Stacl^solution L5g/L
NaN^T»g<^02
i/',
St5^^olutioivf5g/L
X
; *
¦ ~
NO2dub7W-0-5b
8/21/09
125 mL
!We
^ater 1.57 mg/L Nitrite -
DayO
rf2so4
-
X
NO2dub7W-0-6b
8/21/09
125 mL
HDPE
Water 1.57 mg/L Nitrite -
Day 0
Cold
X
Shipper:
Date/Time:
Receiver:
Date/Time:
Temperature:
BODi = Biochemical Oxygen Demand-S day
Nlij-N = Ammonia-Nitrogen
NO3/NO2 -N = N itrate/N i trite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for-other frequently temiested analyjes
NOj-N = Nitrite Nitrogen Total N = Total Nitrogen
NO3 -N - Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of ^
— y
739 Hastings St.
Traverse City, MI 49686
Phone: 231-94 i-2230
Fax: 231-941-2240
wvvw.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
fly • GmV
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below'
Alkalinity.
£3
O
03
Chloride
Chlorophyll a
C/i
-
'¦
. -a-¦:
.
¦-
m
Z
SS
z
z
N
©:
,25
O
Z
TDS
—R:
a,
H
CO
co
: 'H
e#--
:h'
s
NO2dub7W-0-7b
8/21/09
125 mL
HDPE
Water 3.13 mg/'L Nitrite
-Day 0
H2SOi
-
X
N02dub7 W -0-8b
8/21/09
125 mL
HDPE
Water 3.13 mg/L Nitrite
-Day 0
Cold
X
NO2dub7W-0-9b
8/21/09
125 mL
HDPE
Water 6.25 mg/L Nitrite
-Day 0
h2so4
• .
'
X
NO2dub7W-0-
10b
8/21/09
125 mL
HDPE
Water 6.25 mg/L Nitrite
-Day 0
Cold
81III
X
NO2dub7W-0-
11b
8/21/09
125 mL
HDPE
Water 12.5mg/L Nitrite -
Day 0
h2so4
X
NO2dub7W-0-
12b
8/21/09
125 mL
HDPE
Water 12.5mg/L Nitrite -
Day 0
Cold
X
Shipper:
Date/Time:
°KZ)
Receiver:
.
Date/Time:
Temperature:
BODj = Biochemical Oxygen Dcmand-5 day
NHj -H = Ammonia-Nitrogen
NO3/NO2 -N = Nitrate/Nitrite-Nitrogen
Definitions
TOS = Total Dissolve Solids TP = Total Phosphorus
TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOz -N = Nitrite Nitrogen Total N = Total Nitrogen
N03 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
739 Hastings St
Traverse City, Mi 49686
Phone; 231-941-2230
Fax: 231-941-2240
vvww.giec.com
Great Lakes Environmental Center
Facility:
T . Traverse City, Ml
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
lf>N.
1
Date / Time
or Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Cheek the requested analysts (see definitions below'
Bottle ID
J?
.s
<
net
a
0
m
'
•' *E" -
: :Q :
Chlorophyll a
m
.<*>
;<»
" '
--'•CS.'.
u
cs
a '
z
1
M
ac
"z.
25
¦ '
•:Q:
Z .
.6
z
TDS
cu
H
' 03
CO
H
*¦» •
¦ ¦-<*« ¦
.
£
j Nitrite
N02dub7W-l-l
8/22/09
125 mL
HOPE
Mod Hard water (DMW)
control Nitrite-Day 1 old
solution
h2so4
X
N02dub7W-l-2
8/22/09
125 mL
HDPE
Mod Hard water (DMW)
control Nitrite- Day 1 old
solution, c-i
Cold
X
N02dub7W-l-3
8/22/09
125 mL
HOPE
Water mg/L Nitrite -
Day 1 old solution
i,«T)
H2SO4
N02dub7W-l-4
8/22/09
125 mL
HDPE
Water mg/L Nitrite -
Day I old solution
Cold
X
N02dub7W-l-5
8/22/09
125 mL
HDPE
Water tSrSrng/L Nitrite -
Day 1 old solution
¦> ,i*
H2SO4
X"; .
N02dub7W-l -6
8/22/09
125 mL
HDPE
Water-WtS mg/L Nitrite -
Day 1 old solution
Cold
X
Shipper:
Date/Time:
1.
2.
Receiver:
Date/Time:
ft*2V-o<^
Temperature:
BODj = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NCtyNOj -N = Nitrate/Nitrite-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOi -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of>
Oc
D
O
m
*£'•
0
3. .
9
Chlorophyll a
VJ
Wi ¦ ¦¦¦¦
V
C
"O
L.
¦M.. ,
¦~W:
z
ffi
z
55 .
•i.-
O
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8/22/09
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Water 35#-mg/L Nitrite
- Day 1 old solution
t 2.C
h2so4
X-''
NO2duta7W-l-08
8/22/09
125 mL
HDPE
Water 3s3fmg/L Nitrite
- Day 1 old solution
V7 C
Cold
X
NO2dub7W-l-09
8/22/09
125 mL
HDPE
Water S&Qmg/L Nitrite -
Day 1 old solution
l"L.<
H2SO4
X
N02dub7W-l -10
8/22/09
125 mL
HDPE
Water §&©mg/L Nitrite -
Day 1 old solution
•YC
Cold
-
X
N02dub7W-I-l 1
8/22/09
125 mL
HDPE
Water WO mg/L Nitrite-
Day 1 old solution
h2so4
X
N02dub7W-l-12
8/22/09
125 mL
HDPE
Water W©-mg/L Nitrite-
Day 1 old solution
Cold
:
X
Shipper:
Date/Time:
2.
Receiver:
Date/Time:
Temperature:
BODs = Biochemical Oxygen Demand-5 day
NH3 -N = Ammonia-Nitrogen
NO3/NO2 -N = Nitrato'Nitritc-Nitrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjcldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
NOj -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
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CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page I of
739 Hastings St,
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.ttlcc.com
Great Lakes Environmental Center
Facility:
T . Traverse City.
Location: '
MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
1
Mailee Garton/Jamie Saxton
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ID
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
>
Alkalinity
"O
a
0
m
Chloride
Chlorophyll a
of
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8/23/09
125 mL
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Mod Hard water (DMW)
control Nitrite-Day 0
h2so«
x""v-
It
N02dub7W-2-2
8/23/09
125 mL
HDPE
Mod Hard water (DMW)
control Nitrite-Day 0
Cold
- - -
X
N02dub7W-2-3
8/23/09
125 mL
HOPE
Water 1.57 mg/L Nitrite -
Day 2
h2so4
x
tP
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*0 00
N02dub7W-2-4
8/23/09
125 mL
HDPE
Water 1.57 mg/L Nitrite -
Day 2
Cold
VT*> Q.
-Q CD
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"n -j
N02dub7W-2-5
8/23/09
125 mL
HDPE
Water 6.25 mg/L Nitrite -
Day 2
h2so4
X
*D —
m p*
CL re
ro 0
N02dub7W-2-6
8/23/09
125 mL
HDPE
Water 6.25 mg/L Nitrite -
Day 2
Cold
x:
Ship^ei
Date/Time:
Receiver:
i>/>U
Date/Time:
F' Vi 'Oi
Temperature:
3,
BODs = Biochemical Oxygen Demand-5 day
NH3-N - Ammonia-N itrogen
NOj/NOi-N = N itrate/Nitrite-N itrogen
Definitions
TDS = Total Dissolve Solids
TKN = Total Kjeldahl Nitrogen
TP = Total Phosphorus
TSS = Total Suspended Solids
Abbreviations for other ftrouenUv requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
NO3 -N = Nitrate Nitrogen SRP = Solublc Reactive Phosphorus
>
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3
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-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of _
1 EC.
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.gtec.com
Great Lakes Environmental Center
Facility:
, . Traverse City, MI
Location; J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
V- '
a
0
A
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,1
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8/21/09
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Water 1.57 mg/L Nitrite -
Day 3 old solution
h2so4
N02dub7W-3-2
8/2 3/09
\
125 mL
HDPE
Water 1.57 mg/L Nitrite -
Day 3 old solution
Cold
xi: v
' \
-••V.: ;
X
N02dub7W-3-3
8/2|/09
125 mL
HDPE
Water 6.25 mg/L Nitrite -
Day 3 old solution
HpSO,
X
N02dub7W-3-4
8/2^/09
125 tnL
HDPE
Water 6.25 mg/L Nitrite -
Day 3 old solution
Cold
V '•
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X
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X
Shipper:
Date/Time:
BODj = Biochemical Oxygen Demand-5 day
NHj -N = Ammonia-Nitrogen
NO3/NO2-N = Nitrate/Nitrite-Nitrogcn
Definitions
TDS = Total Dissolve Solids
TKN - Total Kjeldahl Nitrogen
Receiver:
& 0*
Date/Time:
7^'2S -01
Temperature:
TP = Total Phosphorus
TSS= Total Suspended Solids
NO2-N = Nitrite Nitrogen
NO; -N = Nitrate Nitrogen
Total N- Total Nitrogen
SRP ™ Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
GleC
sVtNi-t Siiviro-fimctU-i Ci '-V-^
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax:231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
r . Traverse City, MI
Location: *
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mai lee Garton/Jamie Saxtcm
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below
' Alkalinity
~ 1 - \ i •
O
§
Chloride
Chlorophyll a
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w
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8/25/09
125 mL
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Water 3.13 mg/L Nitrite -
Day 4
h2so4
- T
/¦V: Vj.
X
N02dub7W-4-2
8/25/09
125 mL
HDPE
Water 3.13 mg/L Nitrite -
Day 4
Cold
X
N02dub7W-4-3
8/25/09
125 mL
HDPE
Water 12,5 mg/L Nitrite -
Day 4
H2SO4
¦U Ji
W:":
N02dub7W-4-4
8/25/09
125 mL
HDPE
Water 12.5 mg/L Nitrite -
Day 4
Cold
X
Date/Time:
2.
Shipper:
Receiver:
Date/Time:
? -2S-o<
Temperature:
' Definitions
HODs = Biochemical Oxygen Demand-5 day TDS = Total Dissolve Solids TP = Total Phosphorus
NH,-N - Ammonia-Nitrogen TKN = Total Kjeldahl Nitrogen TSS = Total Suspended Solids
NOj/NO.. -N = Nitrale/Nitiite-Nitrogen
Abbreviations for other frequently requested analyses
NO; -N = Nitrite Nitrogen Total N = Total Nitrogen
NO) -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page
of
739 Hastings St.
Traverse City, MI 49686
Phone: 231-941-2230
Fax: 231-941-2240
www.glec.com
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Check the requested analysis (see definitions below)
Bottle ID
Date/Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
' J**"-''
' • ii-
13-
Ck
O
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J Chloride
Chlorophyll a
fh
w>,
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- ::xs
a-
Z.
X
5^
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-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
GleC
Page
of
£jn.s«* Latos €.%>*¦¦;<
739 Hastings St.
Traverse City, MI 49686
Phone:231-941-2230
Fax: 231-941-2240
www.glec.com
Great Lakes Environmental Center
Facility:
. . Traverse City, MI
Location: J
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Cheek
the requested analysis (see definitions below)
Bottle ID
Date / Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Alkalinity
w
Q
O
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Chloride
Chlorophyll a
* Hardness *j
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1
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35
Z
Z.
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-------
CHAIN OF CUSTODY RECORD
(To be completed and submitted with samples)
Page of
GleC
Lafcfas fciwtfcjimii.'nUii Center
739 Hastings St,
Traverse City, MI 49686
Phone; 231-941-2230
Fax:231-941-2240
www.glec.cotn
Facility:
Location:
Great Lakes Environmental Center
Traverse City, MI
Date:
Contact Person:
Email Address:
Phone Number:
Collector:
Mailee Garton/Jamie Saxton
Bottle ID
Date I Time
of Sample
Volume
Collected
Type of
Sample
Container
Sample Description
(Site information /
sample type)
Preservation
Check the requested analysis (see definitions below)
Alkalinity
o
o
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2
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i
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8/22/09
1
125 mL
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Water 6.25 mg/I, Nitrite
- Day 6 old solution
h2so4
. ¦
X
NO2dub7W-6-08
8/2^/09
I
125 mL
HDPE
Water 6.25 mg/L Nitrite
- Day 6 old solution
Cold
X
NO2dub7W-6-09
mim
1
125 mL
HDPE
Water 12.5mg/L Nitrite -
Day 6 old solution
h2so4
X- ' "
N02dub7 W-6-10
8/2|/09
125 mL
HDPE
Water 12.5mg/L Nitrite -
Day 1 old solution
Cold
i-"-
-
X
N02dub7W-6-l 1
8/21/09
1
125 mL
HDPE
Stock solution 1.5g/L
NaNO2-1.0g/LNO2
h2so4
,
¦5* "
x ;
NQ2dub7W-6-12
8/2^/09
125 mL
HDPE
Stock solution 1.5g/L
NaN02-1.0g/L N02
Cold
. V-S
X
Shipper:
Date/Time:
Receiver:
Date/Time:
s-n-oi
Temperature:
BOD5= Biochemical Oxygen Deraand-S day
NH3-N = Ammonia-Nitrogen
NOj/N03 -N = Nitrate/Nitrite-Nitrogen
Definitions :
TDS = Total Dissolve Solids
TKN = TotalKjeldahl Nitrogen
TP = Total Phosphorus
TSS= Total Suspended Solids
Abbreviations for other frequently requested analyses
NOj -N = Nitrite Nitrogen Total N = Total Nitrogen
NG>3 -N = Nitrate Nitrogen SRP = Soluble Reactive Phosphorus
-------
Reference Toxicant Data
-------
Great Lakes Environmental Center
Ceriodaphnia dubia Reproduction
-T- 1 f-% /"* f I ^ • J TT*V
NaCl Reference Toxieant Data
2007-2009
2.5
5-1
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2
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^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ „<# $ ^ $
V # / 4* ^ ^ ^ °* ^
-------
Statistical Analyses
-------
TRIMMED SPEARMAN-KARBER METHOD. MONTANA STATE UNIV
FOR REFERENCE, CITE:
HAMILTON, M.A., R.C. RUSSO, AND R.V. THURSTON, 1977.
TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING MEDIAN
LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS.
ENVIRON. SCI. TECHNOL. 11(7): 714-719;
CORRECTION 12 (4) :417 (1978).
DATE: 8/21/09
CHEMICAL: NITRITE
TEST NUMBER: 6112-12
SPECIES: C.DUBIA
RAW DATA:
CONCENTRATION(MG/L
NUMBER EXPOSED:
1.19
10
2 .70
10
5.76
10
11.70
10
24.00
10
DURATION (HOURS )
TRIM
LC50
LOWER 95% LIMIT
UPPER 95% LIMIT PERCENT
48
. 00
16 . 76
YtJ&cJb ^J \na-0,^
SvvY VvVo-' ^
-------
TRIMMED SPEARMAN-KARBER METHOD. MONTANA STATE UNIV
FOR REFERENCE, CITE:
HAMILTON, M.A., R.C, RUSSO, AND R.V. THURSTON, 1977.
TRIMMED SPEARMAN-KARBER METHOD FOR ESTIMATING MEDIAN
LETHAL CONCENTRATIONS IN TOXICITY BIOASSAYS.
ENVIRON. SCI. TECHNOL. 11 (7) : 714-719;
CORRECTION 12 (4) :417 {1978).
DATE; 8/21/09 TEST NUMBER: 6112-12
CHEMICAL: NITRITE SPECIES: C.DUBIA
RAW DATA:
CONCENTRATION(MG/L
NUMBER EXPOSED:
1.19 2.70 5.76 11.70 24.00
10 10 10 10 10
DURATION (DAYS
LC50 LOWER 95% LIMIT UPPER 95% LIMIT PERCENT
TRIM
7
15 . 60
13 .63
17 . 87
.00
-------
Title: HE CD B-12 NITRITE C. DUBIA REPRODUCTION and Survival
File: N02CDR . Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
D = 791.0889
W = 0.9413
Critical W = 0,9290 (alpha = 0.01 , N = 49)
W = 0.9470 (alpha = 0.05 , N = 49)
Data PASS normality test (alpha = 0.01). Continue analysis.
Title: HECD B-12 NITRITE C. DUBIA REPRODUCTION and. Survival
File: N02CDR . Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
Calculated B1 statistic = 3.6510 {p-value = 0.4553)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B = 13.2767 (alpha = 0.01, df = 4)
= 9.4877 (alpha = 0.05, df = 4)
Using Average Degrees of Freedom
(Based on average replicate size of 9.80)
Calculated B2 statistic = 3.2708 {p-value = 0.5136)
Data PASS B2 homogeneity test at 0.01 level. Continue analysis.
-------
Fisher's Exact Test
NUMBER OF
IDENTIFICATION
ALIVE
DEAD
TOTAL ANIMALS
CONTROL
1.19mg/l
TOTAL
10
10
20
10
10
20
Critical Fisher's value (10,10,10) (alpha=0.05) is 6.0, b value is 10,
Since b is greater than 6.0 there is no significant difference
between CONTROL and TREATMENT at the 0,05 level.
Fisher's Exact Test
NUMBER OF
IDENTIFICATION
ALIVE
DEAD
TOTAL ANIMALS
CONTROL
2,70mg/l
10
10
10
10
TOTAL
20
20
Critical Fisher's value (10,10,10) (alpha=0.05} is 6.0. b value is 10.
Since b is greater than 6.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Fisher's Exact Test
NUMBER OF
IDENTIFICATION
ALIVE
DEAD
TOTAL ANIMALS
CONTROL
10
0
10
-------
5.76mg/l
10
0
10
TOTAL 20 0 20
Critical Fisher's value (10,10,10) (alpha=0.05) is 6.0. b value is 10.
Since b is greater than 6.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 10 0 10
11.7mg/l 9 1 10
TOTAL 19 1 20
Critical Fisher's value (10,10,10) (alpha=0.05) is 6.0. b value is 9.
Since b is greater than 6.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Fisher's Exact Test
NUMBER OF
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
CONTROL 10 0 10
24.0 mg/1 0 10 10
TOTAL 10 10 20
Critical Fisher's value (10,10,10) (alpha=0.05) is 6.0. b value is 0.
Since b is less than or equal to 6.0 there is a significant difference
between CONTROL and TREATMENT at the 0.05 level.
-------
Summary of Fisher's Exact Tests
NUMBER NUMBER SIG
GROUP IDENTIFICATION EXPOSED DEAD 0.05
CONTROL 10 0
1 1.19mg/l 10 0
2 2.70rng/l 10 0
3 5.76mg/l 10 0
4 11.7mg/l 10 1
5 24.0 mg/1 10 10 *
-------
Title: HECD B-12 NITRITE C. DUBIA REPRODUCTION and Survival
File; N02CDR , Transform: NO TRANSFORMATION
Number of Groups; 5
GRP
IDENTIFICATION
REP
VALUE
TRANS VALUE
1
CON
1
17.0000
17.0000
1
CON
2
23.0000
23.0000
1
CON
3
26.0000
26.0000
1
CON
4
28.0000
28 . 0000
1
CON
5
17.0000
17.0000
1
CON
6
29.0000
29.0000
1
CON
7
28 . 0000
28.0000
1
CON
8
31,0000
31.0000
1
CON
9
29.0000
29.0000
1
CON
10
23.0000
23.0000
2
1,
19
MG/L
1
15.0000
15 . 0000
2
1.
19
MG/L
,£>
29.0000
29.0000
2
1.
19
MG/L
3
26.0000
26.0000
2
1,
19
MG/L
4
27.0000
27.0000
2
1.
19
MG/L
5
26.0000
26.0000
2
1.
19
MG/L
6
23 . 0000
23 . 0000
2
1.
19
MG/L
7
28.0000
28.0000
2
1.
19
MG/L
8
28.0000
28.0000
2
1.
19
MG/L
9
25 . 0000
25 .0000
2
1.
19
MG/L
10
24 . 0000
24.0000
3
2 .
70
MG/L
1
24.0000
24.0000
3
2 .
70
MG/L
2
24.0000
24.0000
3
2 .
70
MG/L
3
21.0000
21.0000
3
2 .
70
MG/L
4
21.0000
21.0000
3
2 .
70
MG/L
5
31.0000
31.0000
3
2 .
70
MG/L
6
12,0000
12.0000
3
2 .
70
MG/L
7
22.0000
22,0000
3
2 .
70
MG/L
8
23.0000
23.0000
3
2 .
70
MG/L
9
21.0000
21.0000
4
5 .
76
MG/L
1
20.0000
20.0000
4
5 .
76
MG/L
2
13 . 0000
13 . 0000
4
5.
76
MG/L
3
19.0000
19.0000
4
5 .
76
MG/L
4
19.0000
19.0000
4
5 .
76
MG/L
5
28.0000
28 . 0000
4
5 .
76
MG/L
6
22.0000
22.0000
4
5 .
76
MG/L
1
19.0000
19.0000
4
5 .
76
MG/L
8
20.0000
20.0000
4
5,
76
MG/L
9
18.0000
18.0000
4
5 .
76
MG/L
10
13,0000
13.0000
5
11
. 7
mg/L
1
0 . 0000
0.0000
5
11
.7
mg/L
2
3 . 0000
3.0000
5
11
. 7
mg/L
3
7.0000
7.0000
5
11
..7
mg/L
4
7.0000
7.0000
5
11
,7
mg/L
5
7.0000
7.0000
5
11
.. 7
mg/L
6
0.0000
0.0000
5
11
..7
mg/L
7
3.0000
3 .0000
5
11
,.7
mg/L
8
2.0000
2.0000
5
11
,. 7
mg/L
9
4.0000
4.0000
-------
5
Title; HECD B-12 NITRITE C. DUBIA REPRODUCTION and Survival
File; N02CDR . Transform: NO TRANSFORMATION
Summary Statistics 011 Data TABLE 1 of 2
GRP IDENTIFICATION N MIN MAX MEAN
1
CON
10
17
0000
31
.0000
25.1000
2
1.19 MG/L
10
15
0000
29
0000
25.1000
3
2.70 MG/L
9
12
0000
31
0000
22.1111
4
5.76 MG/L
10
13
0000
28
0000
19.1000
5
11.7 mg/L
10
0
0000
7
0000
3.6000
Title: HECD B-12 NITRITE C. DUBIA REPRODUCTION and Survival
File: N02CDR . Transform: NO TRANSFORMATION
Summary Statistics on Data TABLE 2 of 2
GRP IDENTIFICATION VARIANCE SD SEM C.V. %
1 CON 24.7667 4.9766 1.5737 19.8271
2 1.19 MG/L 16.1000 4.012S 1.2689 15.9860
3 2.70 MG/L 24.1111 4.9103 1.6358 22.2074
4 5.76 MG/L 18.3222 4.2804 1.3536 22.4107
5 11.7 mg/L 7.1556 2.6750 0.8459 74.3052
Title: HECD B-12 NITRITE C. DUBIA REPRODUCTION and Survival
File; N02CDR . Transform: NO TRANSFORMATION
ANOVA Table
SOURCE DF SS MS F
Between 4 3202.8274 800.7069 44.5970
Within (Error) 44 789.9889 17.9543
Total 48 3992.8163
(p-value = 0.0000)
Critical F = 3.7784 (alpha = 0.01, df = 4,44)
= 2.5837 (alpha = 0.05, df = 4,44)
-------
Since F > Critical F REJECT Ho: All equal (alpha = 0.05)
Title; HECD B-12 NITRITE C. DUBIA REPRODUCTION and Survival
File: N02CDR . Transform: NO TRANSFORMATION
Bonferroni t-Test - TABLE 1 OF 2 Ho: Control
-------
Nitrite C. dubia LC25, 20, and 10 for mortality
6
3
3
3
3 :
3
3
control
10
0
0
0.366 mg/L
10
0
0.366
0.82 mg/L
10
1
0.82
1.75 mg/L
10
0
1. 75
3.56 mg/L
10
1
3.56
7.31 mg/L
10
10
7.31
-------
Title; Nitrite C. dubia LC25, 20, and 10 for mortality
File; no2cdic Transform:
NO TRANSFORMATION
Probit
Analysis -
not Using Smoothed
Proportions
NUMBER
NUMBER
OBSERVED
PREDICTED
DOSE
SUBJECTS
OBSERVED
PROPORTION
PROPORTION
0.37
10
0
0.0000
0.0049
0 . 82
10
1
0.. 1000
0.0104
1.75
10
0
0.0000
0.0397
3 .56
10
1
0.1000
0.2522
7.31
10
10
1.0000
0.9434
t. Mu =
4.6718
Est. Sigma
= ^ 1.6652
sd =
0 .5828
sd
0.3 954
Chi-Square lack of fit = 10.1291 Likelihood lack of fit = 6,3811
Table Chi-square = 11.3449 (alpha = 0.01, df = 3)
Table Chi-square = 7.8147 {alpha = 0.05, df = 3)
Title: Nitrite C. dubia LC2 5, 20, and 10 for mortality
File: no2cdlc Transform: NO TRANSFORMATION
Probit LC Estimates
WITHOUT CONTROL DATA
POINT EST. END POINT 95% CONFIDENCE LIMITS
LC 1
0. 7980
-0.8165
2.4125
LC 5
1. 9328
0.6870
3 .1786
LC10
2.5378
1.4342
3.6414
LC2 0
3.2703
2.2577
4.2830
LC2 5
3.5486
2.5421
4.5552
LC3 0
3.7986
2.7833
4.8139
LC4 0
4.2499
3.1863
5.3135
LC50
4.6718
3.5294
5.8141
LC60
5.0936
3.8464
6 . 3409
LC7 0
5 . 5450
4.1632
6.9268
LC7 5
5.7949
4.3310
7.2588
LC80
6.0732
4.5127
7.6338
LC90
6.8058
4.9715
8.6401
LC95
7.4107
5.3353
9.4862
LC99
8 .5455
5.9953
11.0958
-------
MED Toxic Response Analysis Model 10/21/2010 12:57
Log(Exposure Variable)
Parameter Summary (Logistic Equation Regression Analysis)
Parameter
Guess
Final Est
StdError
95%LCL
95%UCL
LogX50
0,3013
0.3853
0.0341
0,3163
0,4544
S
0.7939
2.382
0.498
1.374
3.390
YO
25.10
23,30
1,08
21.12
25.48
Effect Concentration Summary
% Effect
Xp Est
95% LCI
95%UCL
50.0
2.429
2.072
2.847
20.0
1.7371
1.3863
2.1768
10.0
1.4280
1.0677
1,9098
25,0
1,8622
1.5161
2,2873
10121/2010 12:57
MED Toxic Response Analysis Model, Version 1.03
-------
MED Toxic Response Analysis Model 10/21/2010 12:57
Regression Analysis of Variance
Source df SS MS F Alpha
Total 39 3332, 85.4
Regression 2 2575. 1287.5 62.9 0.0000
Error 37 757. 20.5
Data Summary
Exposure
Obs Effects
Pred Effects
Residual
Weight
-0.4365
29.0000
23,2896
-5.7104
1.
-0.4365
28.0000
23,2896
-4.7104
1.
-0.4365
28.0000
23.2896
-4.7104
1.
-0.4365
27.0000
23,2896
-3.7104
1.
-0.4365
26.0000
23,2896
-2.7104
1.
-0.4365
26.0000
23,2896
-2.7104
1.
-0.4365
25.0000
23,2896
-1.7104
1.
-0.4365
24.0000
23,2896
-0.7104
1.
-0.4365
23.0000
23.2896
0.2896
1.
-0.4365
15.0000
23.2896
8.2896
1.
-0.0862
31.0000
23.0410
-7,9590
1.
-0.0862
24.0000
23.0410
-0.9590
1.
-0.0862
24.0000
23.0410
-0,9590
1,
-0.0862
23.0000
23.0410
0.0410
1,
-0.0862
22.0000
23.0410
1.0410
1.
-0.0862
21.0000
23,0410
2.0410
1.
-0.0862
21.0000
23:0410
2.0410
1.
-0.0862
21.0000
23.0410
2.0410
1.
-0.0862
12.0000
23.0410
11,0410
1,
-0.0862
11.0000
23.0410
12.0410
1,
0.2430
28.0000
18.5243
-9.4757
1.
0.2430
22.0000
18.5243
-3,4757
1.
0.2430
20.0000
18,5243
-1.4757
0.2430
20.0000
18.5243
-1.4757
0.2430
19.0000
18.5243
-0.4757
0.2430
19.0000
18.5243
-0.4757
1.
10/21/2010 12:57
MED Toxic Response Analysis Model, Version 1.03
-------
MED Toxic Response Analysis Model 10/21/2010 12:57
i*v4
Data Summary
Exposure
Obs Effects
Pred Effects
Residual
Weight
0,2430
19.0000
18.5243
-0.4757
1.
0.2430
18.0000
18.5243
0.5243
1.
0.2430
13.0000
18.p243
5.5243
1.
0.2430
13.0000
18.5243
5.5243
1.
0.5515
7.0000
•3.&713
-3.0287
1,
0.5515
7.0000
3-;p713
-3.0287
1.
0.5515
7.0000
3.9713
-3,0287
1.
0,5515
4.0000
3.9713
-0.0287
1.
0.5515
3.0000
3,9713
0.9713
1.
0.5515
3.0000
3,9713
0.9713
1.
0.5515
3.0000
3,9713
0.9713
1.
0.5515
2.0000
3.9713
1.9713
1.
0.5515
0.0000
3,9713
3.9713
1.
0.5515
0.0000
3,9713
3.9713
1.
Error Summary
Maximum Iterations Reached Without Convergence
Steepness At Maximum or Minimum Limit
10/21/2010 12:57
MED Toxic Response Analysis Model, Version 1.03
-------
Appendix 18: Raw Data for 96-hour Toxicity of
Boron on Lampsilis siliquoidea
-------
Raw Laboratory Data
-------
9i Hour Acute Toxicity Test Data
Water: gPA- >g 5-2-6,-0*1
treatment:! A~ " '
Sheet
Toxicant 'g>Qt-on
Spacies/Culture #: <>• f
Start Date/Tima: &7§ to ^
mtlals "PS- , A t>
3-o * lb?
J5L
Ohrs
24 hrs
72 hrs
96 hrs
4-|
"7.V P ip--~
~
.#¦
initials
Ohm
24 hrs
% survival
temp.
~lD 0
_E!l
4S
COfKf.
...V.'Q
3. MM"
A*.
Hard.
giL
±s,
Ohrs
JL
a$L
.in
12>o
fe 2 so fyiv
0 hrs
I
\n;
48 hrs
co i
72 hrs
Vo, \
pH
corid
¦fr.iO
iO
4^4
11
Alk
IE
.21
°IO
96 hrs
Jg
L.-na-
tii»
F" pfrr?
rep:
Ohrs
24 hrs
-E
48 hrs
72 hrs
96 hrs
a
TS~
4f
. l
~^T
53H
¦yi?"
96 hrs
V-&-T
Jt-LO
-------
Analytical Chemistry Data
-------
the standard In safety
II.*
unaerwniers
laboratories
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UL-SBN-REP.FO07-O1
Effective Oato: October 6.2008
traiis-iwars t3DiSf§!«rtfi' >r>;
110 s. mmm. sufeM. m mv? -i?c2 jsa
T-. 600 33S-434- /F*. «i t-t
(cover) Page 1 of 1
-------
©
the standard in safety
Underwriters
Laboratories
Client: Illinois Natural History Survey
Attn: Amy Dickinson
1910 South Griffith Drive
Champaign, IL 61820
Laboratory Report
Report:
Priority:
Status.
PWS ID:
228074
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Sample Information
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Client ID
Method
Collected
Collected
Received
ID# 1
Date 1 Time
By:
Date f Time
2091140
1A Central Mussel IN
200.7
06/03/09 00:00
Client
06/18/09 09.15
2091141
IB 31 25oom h
lussel IN
200.7
06/03/09 00:00
a |