United States Environmental Protection Agency	Office of Research and Development

National Exposure Research Laboratory
Research Abstract

Government Performance Results Act Goal: Clean, Safe Water

Significant Research Findings:

Case Studies Illustrating the Stressor Identification Process

Scientific Problem and To achieve the objective of restoring and maintaining the chemical,
Policy Issues	physical and biological integrity of the Nation's waters, numerous

States and Tribes are using biological assessments and biocriteria to
identify water bodies in which the fish, invertebrate, algae or plant
communities, and other aquatic life have been detrimentally impacted
by different single or multiple causes. In many cases, the cause(s) of
these biological impairments has not yet been determined. The
Stressor Identification Guidance Document provides a logical,
scientific process by which State, Tribal, and other water quality
experts can evaluate available information to identify the stressor(s)
causing the biological impairments. Two illustrative cases studies
were developed as part of the Stressor Identification Guidance
Document.

The Guidance Document, which was published under the authority of
Section 304(a)(2) of the Clean Water Act (CWA), is designed to assist
water quality managers in identifying unknown causes of biological
impairments in any type of water body.

Research Approach Potential case studies were submitted by States and U.S. EPA Regional

Offices to the Stressor Identification Workgroup, which is comprised
of members from the States and the U.S. EPA's Office of Research and
Development, Office of Water, and Regional Offices. A study of the
Presumpscot River in Maine was selected to illustrate a relatively
simple case restricted to a short stretch of river. A study of the Little
Scioto River in Ohio was used to demonstrate the causal analysis
process in a situation where multiple causes occurred along several
miles of a river. Data for the cases were provided by the Maine
Department of Environmental Protection and the Ohio Environmental
Protection Agency.

In both cases, data were used to eliminate improbable causes and then
other data from the site, other sites, or studies were evaluated in a

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strength of evidence analysis. All of the data, evidence, and analyses
were presented according to the process described in chapters two
through five of the Stressor Identification Guidance Document.
Invertebrate communities in the Presumscot River were found to be
impaired by total suspended solids. In the Little Scioto River,
embedded substrates were identified as causing biological impairment
in the most upstream location while polyaromatic hydrocarbons and
ammonia and nutrient enrichment were identified as causes in the
middle and most downstream locations.

Results and	By illustrating the principles and processes of the Stressor

Implications	Identification Guidance Document, these case studies should enable

States and Tribes to more easily perform a causal analysis themselves.
Once the causes of the biological impairments are identified, water
resource managers will be better able to locate the sources of the
stressor, or stressors, and take management actions aimed at improving
the biological condition of the water body. This guidance is advisory
in nature and its use is not mandatory. As such, this guidance does not
impose legally-binding requirements on the U.S. EPA, the States,
Tribes, industry, the public, or any other entity.

The U.S. EPA has published the Stressor Identification Guidance
Document under document number EPA-822-B-00-025, dated
December 2000. Paper copies can be obtained from the U.S. EPA
Water Resource Center by phone at (202) 260-7786, by e-mail at
center.water-resource@epa.gov. or through conventional mail by
sending a letter of request to U.S. EPA Water Resource Center, Ariel
Rios Building, 1200 Pennsylvania Ave., Washington, DC 20460.
Copies of the document may also be obtained from the U.S. EPA
National Center for Environmental Publications and Information
(NCEPI) by phone at (513) 489-8190 or through conventional mail at
11029 Kenwood Road, Cincinnati, OH 45242. The document and fact
sheet are also available on the U.S. EPA website at
http://www.epa.gov/OST/biocriteria.

Future Research	Several avenues of research continue to build on the foundation

established by the stressor identification process. Case studies are
being performed to demonstrate how the process works with both
simple and complex situations in a variety of ecosystems.

Associations and other types of evidence are being developed that can
be used in many causal analyses, for instance, the relationship between
excessive nutrients and sensitive biological endpoints such as aquatic
insects. This type of information can be used in many causal
determinations. A Causal Analysis, Diagnosis and Decision
Information System (CADDIS) is being planned that will allow routine

Research
Collaboration and
Publications

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application of the process and put needed data and tools at the
fingertips of resource managers. This tool will help U.S. EPA
Regional Offices, States and Tribes to make rapid, consistent, and
legally defensible identification of causes of water quality impairments
and will help to resolve Total Maximum Daily Load issues.

Contacts for

Additional

Information

Contacts for Additional Information:

Susan Cormier

U.S. EPA, Office of Research and Development
National Exposure Research Laboratory
26 W. Martin Luther King Dr.

Cincinnati, OH 45268

Phone: (513) 569-7995
E-mail: cormier.susan@epa.gov

National Exposure Research Laboratory — October 2001


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