Sample Collection Information Document for Chemicals, Radiochemicals, Pathogens and Biotoxins: Companion to Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2022


EPA/600/R-22/255 | April 2023
https://www.epa.gov/emerqencv-response-research

United States
Environmental Protection
Agency

Sample Collection Information
Document for Chemicals,
Radiochemicals, Pathogens and
Biotoxins

Companion to Selected Analytical Methods for
Environmental Remediation and Recovery
(SAM) 2022

Office of Research and Development
Homeland Security Research Program

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Sample Collection Information Document for
Chemicals, Radiochemicals,
Pathogens and Biotoxins

Companion to Selected Analytical Methods for
Environmental Remediation and Recovery

(SAM) 2022

by

Kathy Hall, Jamie Falik, Matthew Magnuson, Heath Mash, Sanjiv Shah,

Erin Silvestri and Stuart Willison
Center for Environmental Solutions and Emergency Response
Homeland Security and Materials Management Division
Cincinnati, OH 45268

Asja Korajkic and Brian McMinn
Center for Environmental Measurement and Modeling
Watershed and Ecosystem Characterization Division
Cincinnati, OH 45268

John Griggs and Jack Burn
National Analytical Radiation Environmental Laboratory
Office of Air and Radiation, Office of Radiation and Indoor Air
Montgomery, AL 36115

Troy Strock
Office of Land and Emergency Management
Office of Resource Conservation and Recovery
Materials Recovery and Waste Management Division
Arlington, VA 22202

Steve Reimer
U.S. EPA Region 10 - Manchester Laboratory
Port Orchard, WA 98366

Eric Boring, Yildiz Chambers-Velarde, John Chandler,

Joan Cuddeback, Emily King and Robert Rosson
General Dynamics Information Technology, Inc.

3170 Fairview Park
Falls Church, VA 22042

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SAM Companion - Sample Collection Information Document

Disclaimer

The U.S. Environmental Protection Agency (EPA), through its Office of Research and
Development, funded and managed the research described here under Contract EP-C-17-024
to General Dynamics Information Technology (GDIT). The contents reflect the views of the
contributors and technical work groups and do not necessarily reflect the views of the Agency.

Mention of trade names or commercial products in this document or in the methods referenced
in this document does not constitute the Agency's endorsement.

Questions concerning this document or its application should be addressed to:

Erin Silvestri

Homeland Security Research Program
Office of Research and Development (NG16)

U.S. Environmental Protection Agency
26 West Martin Luther King Drive
Cincinnati, OH 45268
(513) 569-7619
silvestri.erin@epa.gov

Jamie Falik (for questions related to the SCID Query Tool)

Homeland Security Research Program

Office of Research and Development (NG16)

U.S. Environmental Protection Agency

26 West Martin Luther King Drive

Cincinnati, OH 45268

(513) 569-7955

falik.iamie@epa.gov

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Foreword

The U.S. Environmental Protection Agency (EPA) is charged by Congress with protecting the
Nation's land, air and water resources. Under a mandate of national environmental laws, the
Agency strives to formulate and implement actions leading to a compatible balance between
human activities and the ability of natural systems to support and nurture life. To meet this
mandate, EPA's research program is providing data and technical support for solving
environmental problems today and building a science knowledge base necessary to manage
our ecological resources wisely, understand how pollutants affect our health, and prevent or
reduce environmental risks in the future.

The Center for Environmental Solutions and Emergency Response (CESER) within the Office of
Research and Development (ORD) conducts applied, stakeholder-driven research and provides
responsive technical support to help solve the Nation's environmental challenges. The Center's
research focuses on innovative approaches to address environmental challenges associated
with the built environment. We develop technologies and decision-support tools to help
safeguard public water systems and groundwater, guide sustainable materials management,
remediate sites from traditional contamination sources and emerging environmental stressors,
and address potential threats from terrorism and natural disasters. CESER collaborates with
both public- and private-sector partners to foster technologies that improve the effectiveness
and reduce the cost of compliance, while anticipating emerging problems. We provide technical
support to EPA regions and programs, states, tribal nations and federal partners, and serve as
the interagency liaison for EPA in homeland security research and technology. The Center is a
leader in providing scientific solutions to protect human health and the environment.

The purpose of the Sample Collection Information Document for Chemicals, Radiochemicals,
Pathogens and Biotoxins is to provide general information (e.g., sample containers,
preservation, size, packaging) to be used by EPA and contractors for collection of samples that
are to be analyzed using the specific methods and procedures listed in EPA's Selected
Analytical Methods for Environmental Remediation and Recovery (SAM) as part of
environmental remediation following an intentional or accidental homeland security-related
contamination incident.

Gregory Sayles, Director

Center for Environmental Solutions and Emergency Response

April 2023

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Table of Contents

DISCLAIMER	I

FOREWORD	II

LIST OF ATTACHMENTS	Ill

ACRONYMS AND ABBREVIATIONS	IV

ACKNOWLEDGMENTS	VII

EXECUTIVE SUMMARY	VIII

1.0 BACKGROUND	1

2.0 SCOPE AND APPLICATION	1

2.1	Sample Collection Information Attachments	2

2.2	Document Development	3

2.3	Limitations	4

3.0 HEALTH AND SAFETY CONSIDERATIONS	4

3.1	Heal th and Safety Plans	5

3.2	Personal Protective Equipment and Monitoring	5

3.3	Training	6

4.0 PREPARATION FOR SAMPLE COLLECTION	6

4.1	Field Sampling Equipment and Supplies	6

4.2	Field Da ta Document a tion	7

4.3	E quipment Opera tion and Calibration	8

5.0 PREPARATION OF SAMPLE CONTAINERS	8

5.1	Sample Container Labels	8

5.2	Preparing Sample Containers for Packaging	8

5.3	General Sample Shipment Guidelines	8

5.4	Chain-of-Custody Forms	9

5.5	Custody Seals/Tamper-evident Bags	9

6.0 DEFINITIONS	10

7.0 BIBLIOGRAPHY	12

List of Attachments

Attachment A: Sample Collection Information for the Chemical Analytes and Methods Listed in
SAM 2022

Attachment B1: Sample Collection Information for the Environmental Sample Types, Radiochemical
Analytes and Methods Listed in SAM 2022

Attachment B2: Sample Collection Information for the Outdoor Building and Infrastructure Materials,
Radiochemical Analytes and Methods Listed in SAM 2022

Attachment C: Sample Collection Information for the Pathogens and Methods Listed in SAM 2022

Attachment D: Sample Collection Information for the Biotoxins and Methods Listed in SAM 2022

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Acronyms and Abbreviations

A-230

Methyl-[1-(diethylamino)ethylidene]-phosphonamidofluoridate

A-232

Methyl-[1-(diethylamino)ethylidene]-phosphoramidofluoridate

A-234

Ethyl N-[(1E)-1-(diethylamino)ethylidene]-phosphoramidofluoridate

AOAC

AOAC International (formerly the Association of Official Analytical Chemists)

Anal. Chem.

Analytical Chemistry

APHA

American Public Health Association

ASTM

ASTM International (formerly American Society for Testing and Materials)

BZ

Quinuclidinyl benzilate

°C

Degree(s) Celsius

CDC

U.S. Centers for Disease Control and Prevention

CESER

Center for Environmental Solutions and Emergency Response (EPA)

CFR

Code of Federal Regulations

cm

Centimeter(s)

cm2

Centimeter(s) squared

COC

Chain of custody

CVAA

2-Chlorovinylarsonous acid

CVAOA

2-Chlorovinylarsonic acid

DGR

Dangerous Goods Regulations

DHS

U.S. Department of Homeland Security

DIMP

Diisopropyl methylphosphonate

DNPH

Dinitrophenylhydrazine

DOE

U.S. Department of Energy

DOL

U.S. Department of Labor

DOT

U.S. Department of Transportation

EA2192

[S-2-(diisopropylamino)ethylmethylphosphonothioic acid]

ECL

Electrochemiluminescence

ED

Ethyldichloroarsine

EDEA

N-Ethyldiethanolamine

ELFA

Enzyme-linked fluorescent immunoassay

ELISA

Enzyme-linked immunosorbent assay

EM PA

Ethyl methylphosphonic acid

EMSL

Environmental Monitoring and Support Laboratory

EPA

U.S. Environmental Protection Agency

ft2

Square foot (square feet)

FDA

U.S. Food and Drug Administration

FRET

Fluorescence resonance energy transfer

FRMAC

Federal Radiological Monitoring and Assessment Center

g

Gram(s)

GA

Tabun

gal

Gallon

GB

Sarin

GD

Soman

GE

1-Methylethyl ester ethylphosphonofluoridic acid

GF

Cyclohexyl sarin

GPS

Global Positioning System

HASL

Health and Safety Laboratory, currently known as Environmental



Measurements Laboratory (EML)

HASP

Health and Safety Plan

HCI

Hydrochloric acid

HD

Mustard, sulfur

Hg

Mercury

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Hg(CN)2

HILIC MS-MS

2-HMP

HMTD

HMX

HN-1

HN-2

HN-3

HN03

HPLC-FL

HSRP

i.d.

I ATA

IMPA

10

ISM

ISO

kPa

L

L-1
L-2
L-3
lbs

LC-ESI-MS-MS

LC-MS

LC-MS-MS

LC-UV

LFA

M

m3

|jL

|jm

MALDI-TOF-MS

MCE

MDEA

mg

min

mL

mm

mM

MPA

NA

Na2CC>3

NaOH

NAREL

NEMI

NH3

NH4CI

NH4OH

NIOSH

n.o.s.

NTT A A

o.d.

Mercury (II) cyanide

Hydrophilic interaction-tandem mass spectrometry

(2-[hydroxymethyl] piperidine)

Hexamethylenetriperoxidediamine

Octa hy d ro-1,3,5,7-tetra n i tro-1,3,5,7-tetrazoci ne

Mustard 1, nitrogen

Mustard 2, nitrogen

Mustard 3, nitrogen

Nitric acid

High performance liquid chromatography-fluorescence
Homeland Security Research Program (EPA)

Inner diameter

International Air Transportation Association
Isopropyl methylphosphonic acid
Inorganic

Inorganic Superfund Methods

International Organization for Standardization

Kilopascals

Liter(s)

Lewisite 1

Lewisite 2

Lewisite 3

Pounds

Liquid chromatography-electrospray ionization-tandem mass spectrometry

Liquid chromatography-mass spectrometry

Liquid chromatography-tandem mass spectrometry

Liquid chromatography-ultraviolet

Lateral flow immunoassay

Molar

Cubic meter(s)

Microliter(s)

Micrometer(s)

Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry

Mixed cellulose ester

N-Methyl diethanolamine

Milligram(s)

Minute(s)

Milliliter(s)

Millimeter(s)

Millimolar

Methylphosphonic acid
Not applicable
Sodium carbonate
Sodium hydroxide

National Air and Radiation Environmental Laboratory (EPA)

National Environmental Methods Index

Ammonia

Ammonium chloride
Ammonium hydroxide

National Institute for Occupational Safety and Health
Not otherwise specified

National Technology Transfer and Advancement Act
Outer diameter

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OMB

ORD

ORISE

OSHA

OVS

OW

oz.

PETN
oa.

PHI LIS

PMPA

ppbv

PPE

ppm

pptv

psig

PSN

PTFE

PUF

PVC

QC

R-33

RDX

RESL

Rev

SAM

SCID

SCP

SM

SOP

SRS

SW

TBD

TDG

TEA

TEPP

TETS

Tl

1.3.5-TNB

2.4.6-TNT
TO

UN#

USGS

VCSB

VE

VG

VM

VOA

Vol.

VX

Office of Management and Budget Directives
Office of Research and Development (EPA)

Oak Ridge Institute for Science and Education
Occupational Safety and Health Administration
OSHA versatile sampler
Office of Water (EPA)

Ounce(s)

Pentaerythritol tetranitrate
Page(s)

Portable High-Throughput Integrated Laboratory Identification Systems

Pinacolyl methyl phosphonic acid

Parts-per-billion by volume

Personal protective equipment

Parts per million

Parts per trillion by volume

Pounds per square inch gauge

Proper shipping name

Polytetrafluoroethylene

Polyurethane foam

Polyvinyl chloride

Quality control

Methylphosphonothioic acid, S-[2-(diethylamino)ethyl]0-2-methylpropyl ester]

Hexahydro-1,3,5-trinitro-1,3,5-triazine

Radiological and Environmental Sciences Laboratory

Revision

Selected Analytical Methods for Environmental Remediation and Recovery
Sample Collection Information Document
Sample Collection Plan

Standard Methods for the Examination of Water and Wastewater
Standard Operating Procedure

Savannah River National Laboratory, Savannah River Site

Solid Waste

To be determined

Thiodiglycol

Triethanolamine

Tetraethyl pyrophosphate

T etramethylenedisulfotetramine

Transportation Index

1.3.5-T	rinitrobenzene

2.4.6-Trinitrotoluene
Toxic organics

United Nations numbers
United States Geological Survey
Voluntary Consensus Standards Body

Phosphonothioic acid, ethyl-, S-(2-(diethylamino)ethyl) O-ethyl ester
Phosphorothioic acid, S-(2-(diethylamino)ethyl) 0,0-diethyl ester
Phosphonothioic acid, methyl-,S-(2-(diethylamino)ethyl) O-ethyl ester
Volatile Organic Analysis
Volume

0-ethyl-S-(2-diisopropylaminoethyl) methyl- phosphonothiolate

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Acknowledgments

This document was developed by the U.S. Environmental Protection Agency's (EPA's)
Homeland Security Research Program (HSRP) within EPA's Office of Research and
Development as a companion to Selected Analytical Methods for Environmental Remediation
and Recovery (SAM) 2022. The document was prepared by General Dynamics Information
Technology (GDIT) under EPA Contract No. EP-C-17-024. Contributions of the following
individuals and organizations to the development of the information provided in this document
are acknowledged:

United States Environmental Protection Agency (EPA)

•	Office of Research and Development (ORD)

o Center for Environmental Solutions and Emergency Response (CESER)

Angela Batt
Veera Boddu
Kathy Hall
Matthew Magnuson
Heath Mash
Sanjiv Shah
Ramona Sherman
Erin Silvestri
Stuart Willison

o Center for Environmental Measurement and Modeling (CEMM)

Asja Korajkic
Brian McMinn

•	Office of Air and Radiation, Office of Radiation and Indoor Air (ORIA), National
Analytical Radiation Environmental Laboratory (NAREL)

Jack Burn
John Griggs
Joshua Pate

•	Office of Land and Emergency Management (OLEM)

Troy Strock (Office of Resource Conservation and Recovery)

Christine Tomlinson (Office of Emergency Management)

EPA Regions

Steve Reimer (Region 10)

Centers for Disease Control and Prevention

Mia Mattioli (National Center for Emerging and Zoonotic Infectious Diseases)

U.S. Department of Agriculture

Marcus Head (Food Emergency Response Network [FERN] National Programs Office)

U.S. Food and Drug Administration

Derek Peloquin (Office of Regulatory Affairs, Forensic Chemistry Division)

Ohio Department of Health

Rashmi Gaur

General Dynamics Information Technology (GDIT)

Eric Boring	Emily King

Yildiz Chambers-Velarde	Robert Rosson

John Chandler
Joshua Criss
Joan Cuddeback.

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Executive Summary

The Sample Collection Information Document for Chemicals, Radiochemicals, Pathogens and
Biotoxins is a companion to EPA's Selected Analytical Methods for Environmental Remediation
and Recovery (SAM) and provides general information for collection of samples that are to be
analyzed using the specific methods and procedures listed in SAM. This document provides
information regarding sample containers, preservation, size, packaging and sources for
additional information that can be used by EPA and EPA contractors when collecting samples in
support of environmental remediation following an intentional or accidental homeland security-
related contamination incident. It can also be used as a tool to assist other federal agencies and
state and local response partners when collecting samples that are to be analyzed using SAM
methods.

This document provides only summary information regarding collection of samples to be
analyzed for measurement of the chemicals, radiochemicals, pathogens and biotoxins using the
methods and procedures listed in SAM 2022. It does not provide details regarding sample
collection procedures or the corresponding analytical methods. Although at this time not all of
the information has been evaluated for every analyte or sample type, the information listed is
considered by the SAM work groups to be the most appropriate and available at the time of
publication. Many of the target analytes listed in this document have only recently become an
environmental concern, and EPA is actively pursuing development and validation of appropriate
sample collection procedures. The information will be reviewed and updated periodically, along
with the selected methods in SAM, to reflect advances in technologies, results of method
evaluation and validation studies, and additional analytes or matrices.

Product Development Quality Assurance

The information in this document is based on secondary sources, including peer reviewed
scientific methods cited in EPA's Selected Analytical Methods for Environmental Remediation
and Recovery (SAM). Where possible, the information provided was obtained from the
sample collection requirements and guidelines included in the analytical methods listed in
SAM. Where this information was not available, additional sources were used, including the
Code of Federal Regulations (CFR) and nationally-recognized scientific, technical or
response organizations. Information sources are cited in Section 7.0 and throughout
Attachments A through D.

The document completed several review cycles prior to publication, including EPA project
lead review, technical workgroup reviews, internal EPA technical review, Homeland Security
and Materials Management Division (HSMMD) quality assurance and technical edit reviews,
external technical review, and HSMMD management reviews. All comments from reviewers
have been tracked and are maintained by EPA, along with the revisions and adjustments
made to address the comments.

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Sample Collection Information Document
for Chemicals, Radiochemicals, Pathogens and Biotoxins

[Companion to Selected Analytical Methods for Environmental Remediation

and Recovery (SAM) 2022]

1.0 Background

The information contained in this document is intended to support and be used with
the methods listed in EPA's Selected Analytical Methods for Environmental
Remediation and Recovery (SAM) 2022 document. This document provides summary
information regarding collection of samples that are to be analyzed using the specific
methods and procedures listed in SAM 2022 and does not provide details regarding
sample collection procedures or the corresponding SAM methods.

The U.S. Environmental Protection Agency's (EPA's) Homeland Security Research
Program (HSRP) has worked with experts across EPA and other interested agencies
since 2003 to develop a compendium of analytical methods to be used in support of
remediation activities following homeland security-related contamination incidents.1
Methods and protocols have been selected for chemical, radiochemical, pathogen and
biotoxin analytes of concern in environmental sample matrices that are anticipated in
such incidents. Methods and protocols have also been selected for radiochemical
analytes of concern in outdoor building and infrastructure materials containing
radiochemical contamination. The results of these efforts have been published in
several revisions of EPA's Selected Analytical Methods for Environmental Remediation
and Recovery (SAM).2 HSRP periodically reviews and updates this information to
address the needs of homeland security, reflect improvements in analytical methods
and new technologies, and incorporate changes in target analytes.

In support of efforts to select appropriate analytical methods, EPA also recognizes the
need for companion documents to provide information regarding collection of samples
for analysis using the methods in SAM. This document is intended to address this need,
in part, by providing general information regarding sample containers, preservation,
size, packaging, and sources for additional information supporting collection of samples
to be analyzed for chemicals, radiochemicals, pathogens or biotoxins. As with SAM
2022, HSRP plans to update the information in this document periodically, to reflect
changes to the analytes and/or methods that have been selected.

2.0 Scope and Application

This Sample Collection Information Document (SCID) provides general information for
use by EPA and its contractors when collecting samples during environmental
remediation following a contamination incident. The document is intended to be used

1	For the purposes of this document, homeland security-related incidents encompass human-made
contamination (whether intentional or unintentional), natural disasters and epidemics that impact or
threaten the safety, security and resiliency of the United States.

2	Selected Analytical Methods for Environmental Remediation and Recovery (SAM) and its methods are
available at: https://www.epa.gov/esam/selected-analvtical-methods-environmental-remediation-and-
recoverv-sam.

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with SAM 2022 (EPA/600/R-21/320; U.S. EPA, 2022) and to provide information
needed for collection of samples to be analyzed using the specific methods and
procedures that have been selected for chemicals, radiochemicals, pathogens and
biotoxins. Where possible, the information provided was obtained from the sample
collection requirements and guidelines included in the analytical methods. Where this
information was not available, additional sources were used (see Section 7.0). This
document is considered by the SAM work groups to be representative of the most
appropriate and current information available at the time of publication.

The information in this document addresses the analytes and sample types included in
SAM 2022 (see Attachments A - D for specific analytes and sample types) and is
intended to be used to support the following during site remediation:

Assessment: Determine the extent of site contamination (assumes early
responders have identified contaminants prior to EPA's remediation effort)
Cleanup: Assess the remediation efforts during the site cleanup process
Clearance: Confirm the contamination level in support of site clearance
decisions

It assumes that samples will be collected by personnel trained in the collection of
environmental samples suspected of containing the target analytes and the appropriate
safety procedures (see Section 3.0). Sample collection personnel should consult the
site- or incident-specific documentation (e.g., Sample Collection Plans [SCPs]) and/or
receiving laboratory(ies) prior to initiating sample collection activities.

NOTE: It is possible that some of the information in this document should be modified to
address site- or incident-specific data needs; for example, additional sample volume may
be needed for quality control (QC) or in cases where a low level of the analyte is anticipated
in the sample. Documentation should be in place and consulted for specific sample
collection requirements prior to initiation of sample collection activities. For example, site- or
incident-specific SCPs include information regarding laboratory capacity; the extent of
contamination; target analytes, including their preservation and holding times; data quality
objectives; sample locations; and the number and type of samples needed.

2.1 Sample Collection Information Attachments

This document contains the following attachments listing information for
collection of samples that will be analyzed for measurement of the analytes
listed in Selected Analytical Methods for Environmental Remediation and
Recovery:

•	Attachment A: Sample Collection Information for the Chemical Analytes and
Methods Listed in SAM 2022

•	Attachment B1: Sample Collection Information for the Environmental
Samples, Radiochemical Analytes and Methods Listed in SAM 2022

•	Attachment B2: Sample Collection Information for the Outdoor Building and
Infrastructure Materials, Radiochemical Analytes and Methods Listed in SAM
2022

•	Attachment C: Sample Collection Information for the Pathogens and Methods
Listed in SAM 2022

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• Attachment D: Sample Collection Information for the Biotoxins and Methods
Listed in SAM 2022

Each attachment provides the minimum sample size that should be collected to
support sample analysis, the preservatives and/or temperature, the maximum
amount of time that should elapse between sample collection and the initiation of
analytical procedures (e.g., sample analysis, digestion or extraction), the
appropriate type of container, general sample labeling and packaging procedures
needed for sample shipment, and the source(s) used to provide the information.
The information in each attachment is arranged by analyte and corresponds to
the sample types that are addressed by the methods that have been selected for
inclusion in SAM 2022.

2.2 Document Development

EPA employed several references to identify the information that is included in
this document. The first sources consulted were the methods listed in SAM 2022.
If the SAM 2022 methods included sample collection information, the information
was evaluated and, if appropriate, included in the sample collection information
tables provided in Attachments A, B1, B2, C and D. Secondary sources
consulted included EPA procedures for collection of samples addressing the
specific analyte/matrix pairs. If there were no EPA procedures available, other
federal agency or Voluntary Consensus Standards Body (VCSB)3 methods were
consulted, and best professional judgment applied by technical work groups to
address collection of each of the analyte/sample type pairs. In many cases,
information was adjusted for consistency across similar analytes (e.g., volatile
organic compounds) and sample types, to facilitate sample collection without
compromising analytical results.

Information from the following agencies, organizations and publications was
evaluated:

• EPA - United States Environmental Protection Agency

• AOAC - AOAC International (formerly Association of Analytical Chemists)

• ASTM - ASTM International (formerly American Society for Testing and
Materials)

• CFR - Code of Federal Regulations

• U.S. CDC - United States Centers for Disease Control and Prevention

• USDA - United States Department of Agriculture

• U.S. DHS - United States Department of Homeland Security

• U.S. DOE - United States Department of Energy

• U.S. DOL - United States Department of Labor

• U.S. DOT - United States Department of Transportation

• U.S. FDA - United States Food and Drug Administration

3 EPA OMB Circular No. A-119, February 1998. VCSBs are domestic or international organizations which
plan, develop, establish, or coordinate voluntary consensus standards using agreed-upon procedures.
Under the National Technology Transfer and Advancement Act (NTTAA), participation of federal
representatives in VCSBs is encouraged to increase the likelihood that the standards they develop will
meet both public- and private-sector needs.

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•	USGS - United States Geological Survey

•	IATA- International Air Transport Association

•	ISO - International Organization for Standardization

•	NEMI - National Environmental Methods Index

•	NIOSH - National Institute for Occupational Safety and Health

•	OPCW - Organisation for the Prohibition of Chemical Weapons

•	OSHA - Occupational Safety and Health Administration

•	Standard Methods for the Examination of Water and Wastewater, 23rd
Edition. 2017. American Public Health Association

•	Scientific, peer-reviewed journals (see Attachments A through D for cited
journals)

Sample collection information was not identified for all the analyte/sample type
pairs listed in SAM 2022. In some cases, "TBD" (to be determined) is used to
identify information gaps. In other cases, analytes are listed as not a concern in a
particular sample type; in these cases, SAM work groups have determined that
the analyte is not a concern due to a number of factors, including low likelihood
of persistence, toxicity, mobility or solubility.

2.3 Limitations

This document provides summary information only regarding collection of
samples to be analyzed for measurement of the chemicals, radiochemicals,
pathogens and biotoxins listed in SAM 2022. It does not provide details that
would be included in documented sample collection procedures or the
corresponding analytical methods. Although at this time not all of the information
has been tested for every analyte or sample type, SAM work groups consider the
information listed to be the most appropriate and available at the time of
publication. Many of the target analytes listed in this document have only recently
become an environmental concern, and EPA is actively pursuing development
and validation of appropriate sample collection procedures. Users should consult
EPA's Environmental Sampling and Analytical Methods (ESAM) Program
website for periodic updates: https://www.epa.gov/esam.

Site- or incident-specific documentation must be consulted for requirements,
including QC and reporting. The information sources cited in this document also
should be consulted for additional details regarding sample collection, including
QC requirements, sample handling, packaging, shipping and safety procedures.
Sample collection personnel should check with the incident commanders for
special instructions regarding evidentiary matters prior to sample collection.

3.0 Health and Safety Considerations

This document does not address all health and safety issues associated with sample
collection. The importance of training, medical monitoring, required vaccinations (if
applicable) and information included in the site- or incident-specific Health and Safety
Plan (HASP) should be emphasized. In addition to potential harm posed to the
individuals involved, unsafe conditions in the field can indirectly impact the resulting
analytical data. This document assumes a site- or incident-specific HASP is in place that
includes the safety concerns and requirements regarding the specific types of hazards to
be considered during sample collection. This section provides general guidelines
regarding health and safety concerns. Sampling personnel can refer to their site-specific

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HASP for health, safety and personal protective equipment (PPE) considerations
specific to the sample collection event.

3.1 Health and Safety Plans

Health and Safety Plans will vary depending on the site, the sampling phase (site
assessment, remediation or final clearance status determination), and the
responsible organization. The purpose of these plans is to ensure maximum
protection to workers, the environment and surrounding communities, in a way
that is consistent with requirements needed to perform operational activities.
HASPs should follow OSHA guidelines (https://www.osha.gov/emergency-
preparedness/hazardous-waste-operations; accessed January 2023). At a
minimum, HASPs should include instructions and guidelines regarding:

Names, positions and contact information of key personnel and of health and
safety personnel

Site- or incident-specific risk assessment addressing sample collection
activities

•	Training requirements

PPE on site and usage/maintenance requirements

Medical screening requirements (maintain confidential documents properly

and securely)

Site or incident control

•	The emergency response plan, containing off-site emergency contact
information such as local hazardous materials response teams or additional
trained rescue personnel (29 CFR 1910.38)

Entry and egress procedures
Spill containment
Decontamination procedures

•	Waste disposal

NOTE: Entry and decontamination procedures for contaminated areas should
address personnel monitoring and decontamination during entry and egress.

3.2 Personal Protective Equipment and Monitoring

Each site or incident will dictate the level of PPE that will be required. Selection
of protective clothing is dependent on the site conditions and sample collection
requirements included in the SCP. Specific guidance for selection of PPE is
provided in 29 CFR 1910.120, Appendix B. Factors that should be considered
during selection include: contaminant properties, routes of exposure (i.e.,
inhalation, skin absorption, ingestion and injection), performance of equipment in
protecting against exposure, activity duration, and stress induced by work
requirements. Because the use of PPE can also cause hazards to workers (e.g.,
heat stress, impaired vision and mobility), care should be taken to provide a level
of protection that is sufficient to prevent exposure yet is not so high as to create
other unnecessary hazards.

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3.3 Training

Sampling personnel must be trained in collection and handling of samples
suspected of containing the contaminants of concern (see Attachments A, B1,
B2, C and D) and must be up-to-date regarding medical screening requirements.
At a minimum, all sampling team members should be trained in Occupational
Safety and Health Administration (OSHA) requirements for hazardous waste
operations and emergency response per 29 CFR 1910.120 or 29 CFR 1926.65
and have current medical screening, as appropriate. Additionally, sampling
personnel must be trained in and comply with the site HASP and all site-specific
training requirements, including the following:

•	Ability to select and work with the appropriate level of PPE

•	Ability to utilize personnel monitoring equipment
Decontamination procedures

Prevention of sample cross-contamination
Training frequency and documentation procedures

4.0 Preparation for Sample Collection

It is highly recommended that sampling kits be used during sample collection, and that
these kits be properly equipped, maintained and organized before deployment of sample
collection personnel. These kits should be checked for quality assurance prior to
initiation of sample collection activities. Sampling personnel should consult with project
managers and the SCP to determine what equipment and materials should be
assembled, whether samples should be preserved in the field, and how sample
containers will be packaged for shipment. Prior to sample collection, sampling personnel
should contact the laboratory that will be analyzing the samples to discuss laboratory-
specific requirements. Sample kits should contain all containers, materials, supplies and
forms needed to perform sample collection, decontamination, documentation and field
packaging activities. Procedures for preparing samples for shipment should be in place
and understood.

4.1 Field Sampling Equipment and Supplies

Before starting field sampling activities, all necessary equipment and supplies
should be checked, identified and available. The following is a preliminary list:

Sampling devices (e.g., soil coring device, water autosampler, bottles, filters,
air filter canister or collection tube, wipes)

Preservatives and dechlorinating agents (if feasible, it is recommended that
preservatives and dechlorinating agents be added to sample containers prior
to sample collection)

Sample volumetric measuring devices and/or weighing devices
Sample containers and packaging materials, including wet ice or ice packs
(ensure ice does not come into direct contact with samples)

•	PPE

Record keeping devices (e.g., logbooks, chain-of-custody [COC] forms,
writing instruments, camera)

Site maps, Global Positioning System (GPS) recorders, etc.

Sample location markers

Shipping containers, shipping forms and shipping labels
Waste containers

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Materials for cleaning equipment and sample container exteriors
Field testing equipment (e.g., dissolved oxygen, pH, conductivity meters)

Samples should be collected using dedicated, sterile or sufficiently clean
sampling equipment to minimize interferences and cross-contamination. In some
cases, pre-packaged equipment and materials are available and can be used for
efficiency. When disposable equipment is not used, contaminated equipment
must be appropriately cleaned and decontaminated prior to reuse.

4.2 Field Data Documentation

All data collected in the field should be adequately documented. Documented
information should include (for example):

Names of field sampling personnel
Sample Collection Plan (SCP)

Sample location(s) (including GPS coordinates, if appropriate)

Date and time of sampling
Sample identification number
Sampling depth

Sample type and collection methods used

Sample collection medium

Physical and meteorological conditions

Expected contaminants

Expected radionuclides (if applicable)

Sample size (weight, volume), sample duration (air filters), air volume,

preservation, etc.

Sample handling precautions

Field screening data (if applicable)

Radiological dose rate measurements of samples performed in the field should
be documented and transmitted to the laboratory along with the samples.
Documentation should include the background count rates; units; and the type,
serial number and calibration date of the instrument used to obtain the dose rate.

Electronic data recording devices are also available for use, such as Scribe4 and
RadResponder,5 to collect, store and report sampling and analytical data.

4 Scribe is a software tool developed by EPA's Environmental Response Team to assist in managing
environmental data. It includes functionality to support sample documentation, including sample labels,

COC, and laboratory data reports. For additional information regarding this tool see:

https://www.epa.gov/ert/ert-information-management (accessed April 2023).

5 RadResponder is a product of collaboration between the Federal Emergency Management Agency
(FEMA), DOE/National Nuclear Security Administration (NNSA), EPA, the Defense Threat Reduction
Agency (DTRA), and the Department of Homeland Security's Science and Technology Directorate
(DHS S&T), and is provided free of charge to all federal, state, local, tribal, and territorial response
organizations. RadResponder is available at: https://www.radresponder.net (accessed January 2023).

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4.3 Equipment Operation and Calibration

Sampling equipment should be verified to be in working order before it is
deployed with the sampling teams. Equipment should be operated in accordance
with the manufacturer's operating instructions (unless otherwise specified in the
SCP). Equipment also should undergo routine maintenance and be calibrated
according to manufacturer instructions, and the calibration documented prior to
use.

5.0 Preparation of Sample Containers

5.1	Sample Container Labels

Each sample container has a label that provides information uniquely identifying
and describing the sample. A single, unique label is affixed to each individual
sample container, sample information is added in waterproof ink, and the label is
covered with clear tape. Alternatively, waterproof and chemical resistant labels or
a pre-prepared label that uniquely identifies the sample, such as a bar/quick
response (QR) code that tracks the sample information, can be affixed to the
container. Sample container labels include information regarding the date and
time of sample collection; sample collection site; date, time and type of sample
preservation; sample identification numbers; and the names and signatures of
sampling personnel.

5.2	Preparing Sample Containers for Packaging

Once samples have been collected and preserved as specified, the containers
are prepared for shipment to the analytical laboratory. Sample containers should
be labeled, sealed, decontaminated and/or disinfected, and other appropriate
surveys completed (radiochemical samples) prior to packing into transport or
shipping containers. Sample collection personnel should consult the incident-
and site-specific documentation and/or analytical laboratory regarding
appropriate secondary containment. Summary procedures for cleaning and
sealing sample containers are provided in the tables included in Attachments A,
B1, B2, C and D.

5.3	General Sample Shipment Guidelines

Sampling personnel are responsible for ensuring compliance with DOT and IATA
regulations regarding the transfer of hazardous substances and environmental
samples. Regulations 49 CFR 172 and 173 for DOT (organized by separate
subparts as references in Section 7.0), and the Dangerous Goods Regulations
(DGR) for IATA, provide specific details regarding proper marking, labeling,
placarding, packaging and shipment of hazardous materials, substances and
wastes. Regulatory exceptions, such as those for shipping environmental
samples, are also included. These regulations must be consulted prior to
preparation of or planning for sample shipment. Only trained and certified
shippers may ship hazardous materials. Summary information regarding
appropriate labeling and packaging of sample transport containers is included in
Attachments A, B1, B2, C and D.

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5.4	Chain-of-Custody Forms

COC forms create a written record that can be used to trace the creation,
possession and handling of the sample from the moment of its collection through
analysis. A COC form accompanies each sample or group of samples as custody
of the sample(s) is transferred. One copy of the form is retained by sampling
personnel. If multiple laboratories are receiving a sample, individual COCs are
provided to each individual laboratory, with each COC representing the contents
of the sample shipment. Sampling personnel are responsible for the initial
maintenance and completion of COC forms and should place forms in protective
covering to ensure their integrity is maintained throughout transport. Although
COC forms vary in style, format and detail, the forms should contain the same
minimal information required to identify the sample and to document custody.

At a minimum, sampling personnel are responsible for providing the following
information on the COC form:

General incident information (e.g., sample owners, contact information, site
name)

Sample information (e.g., sample identification number, sample type [matrix],

whether grab or composite, number and type of sample containers, whether

preservatives were added, sample locations, sample volumes, and date/time

sample was collected)

Date and time the sample was relinquished

Signature of persons transferring and receiving the samples

5.5	Custody Seals / Tamper-evident Bags

Custody seals are attached over the cap of each sample container to ensure the
sample has not been opened or tampered with after collection and packaging. A
custody seal also can be placed over the shipping or transport container, making
it impossible to open the container without ripping the seal. Typically, there is one
seal per sample container and two seals are placed on opposite sides of the
shipping container. Custody seals contain the signature of the person
responsible for packing the container and the date (and if requested, time)
sealed. The seal must be sturdy to resist incidental contact but able to break
when the cap or lid is removed. Sampling personnel should:

Sign and date the sample custody seal using waterproof ink, usually a 1- by
3-inch white paper label with black lettering and an adhesive backing. The
custody seal is part of the COC process and is used to prevent or identify
tampering with samples.

Place the custody seal across the container lid so that the seal will be broken
if a container is opened. If a cooler is used, ensure that the water drainage
point is secure.

Alternatively, sample containers may be placed into a tamper-evident bag.
Bags/seals with a unique identifier must be associated with the person collecting
the sample through logbook entries, preservation of tear-off strips in the logbook,
or similar means. When using a tape-type tamper-evident seal, the initials of the
person securing the container and the date that it was secured must be recorded
on the seal.

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6.0 Definitions

The following definitions are provided to describe the specific sample collection
information listed in the tables in Attachments A, B1, B2, C and D:

• Analyte - The chemical compound, radionuclide, pathogen, or biotoxin that will be
analyzed/measured in the sample. The analytes in this document are intended to
address the analytes listed in SAM 2022.

Selected Analytical Method - The analytical procedures selected by technical work
group members to address the analytes in SAM 2022.

Tiers are provided for the chemistry methods cited in Attachment A, the pathogen
methods cited in Attachment C, and the biotoxin methods cited in Attachment D, to
indicate the level of method usability assigned for the specific analyte and sample
type. Method usability tiers have not been assigned to the radiochemistry methods
cited in Attachments B1 and B2.

-	For chemistry methods, these tiers are described in SAM 2022 as:

Tier I: Analyte/sample type is a target of the method(s). Data are available for all
aspects of method performance and quality control measures supporting its use
for analysis of environmental samples following a contamination incident.
Evaluation and/or use of the method(s) in multiple laboratories indicate that the
method can be implemented with no additional modifications for the
analyte/sample type.

Tier II: (1) The analyte/sample type is a target of the method(s) and the
method(s) has been evaluated for the analyte/sample type by one or more
laboratories, or (2) the analyte/sample type is not a target of the method(s), but
the method has been used by laboratories to address the analyte/sample type. In
either case, available data and/or information indicate that modifications will likely
be needed for use of the method(s) to address the analyte/sample type.

Tier III: The analyte/sample type is not a target of the method(s), and/or no
reliable data supporting the method's fitness for its intended use are available.
Data from other analytes or sample types, however, suggest that the method(s),
with significant modification, may be applicable.

-	For pathogen methods, these tiers are described in SAM 2022 as:

Tier I: The method was developed for the pathogen and sample type. The
method has been evaluated by multiple laboratories, a detailed protocol has
been developed, and suitable QC measures and checks are provided.

(Examples: EPA Method 1623.1 [Cryptosporidium in water]; Standard Methods
9260 E [Shigella culture method].)

Tier II: The pathogen is the target of the method, and the method has been
evaluated by one or more laboratories. The available data and/or information
indicate that additional testing and/or modifications will likely be needed.
(Example: Cunningham et al., 2010 [Shigella molecular method].)

Tier III: The pathogen is not the target of the method but the method is for the
specific sample type and the pathogen is similar to the target of the method (i.e.,
vegetative bacteria, spore-forming bacteria, virus or protozoan). Data and expert
opinion suggest, however, that the method(s) may be applicable with

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modifications. (Example: EPA Yersinia pestis protocol for Chlamydophila psittaci
in water.)

-	For biotoxin methods, these tiers are described in SAM 2022 as:

Tier I: The biotoxin and sample type are both targets of the method(s). Data are
available for all aspects of method performance and QC measures supporting its
use without modifications.

Tier II: The biotoxin is a target of the method, and the method has been
evaluated by one or more laboratories. The sample type may or may not be a
target of the method, and available data and/or information regarding sample
preparation indicate that analyses of similar sample types were successful.
However, additional testing and/or modifications may be needed.

Tier III: The sample type is not a target of the method, and no reliable data
supporting the method's fitness for its intended use are available. Data suggest,
however, that the method(s) may be applicable with significant modification.

Method types are provided for the radiochemistry methods cited in Attachment B1
and the biotoxin methods cited in Attachment D, to indicate the intended analytical
purpose.

-	For radiochemistry methods, these method types are described in SAM 2022 as:

Qualitative: The identified method is intended to determine the presence of a
radionuclide. Although quantitative, these methods have been selected as
qualitative methods since they can be utilized with shorter counting times, at
greater uncertainty, when increased sample throughput and more rapid reporting
of results are required.

Confirmatory: The identified method is for measurement of the activity from a
particular radionuclide per unit of mass, volume or area sampled.

-	For biotoxin methods, these method types are described in SAM 2022 as:

Presumptive: Presumptive methods are generally more rapid than confirmatory
methods, and might be used during the initial stages of remediation to evaluate
the extent of contamination. Presumptive methods also might be used to identify
samples that should be analyzed using the more extensive confirmatory
methods, and can be adapted for large-scale sample analysis while maintaining
an appropriate level of analytical certainty.

Confirmatory: Confirmatory methods are generally more time consuming and
expensive, and are intended to provide results with a higher level of certainty
than those provided by presumptive methods. These methods should be
considered for use when presumptive analysis indicates the presence of the
biotoxin, a smaller subset of samples requires analysis, or as required for a tiered
approach to remediation.

Biological Activity: Methods that address biological activity tend to be time
consuming and expensive, and are intended to provide a high level of certainty in
corroborating results of presumptive and/or confirmatory assays. Depending on
the goals of the remediation phase, biological activity methods may also be
needed to determine bioactivity of a given biotoxin detected by a presumptive
and/or confirmatory assay that responds to both inactive and biologically active
biotoxins.

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Sample Container - The type of container (e.g., bottle, bag) that must be used to
hold the sample. The container must be sufficient to maintain sample integrity and be
composed of materials that will remain inert when in contact with the sample.

Holding Time - The maximum amount of time allowable from sample collection until
sample analysis, extraction or inoculation. The actual holding time depends on the
target analyte(s), the analytical method that will be used, and the data requirements
for the contamination incident. Sampling personnel should consult the site- and
incident-specific documentation and/or receiving laboratory for confirmation.

Packaging - Sample container packaging requirements for shipment of samples to
the laboratory.

Preservation - Conditions and/or chemicals used to maintain the integrity of a
sample. Some examples of common preservatives for samples containing biotoxins,
chemical, or radiochemical analytes include, but are not limited to nitric acid,
hydrochloric acid, or sodium thiosulfate; preservation of samples containing biotoxins
and pathogens often requires temperatures < 10 °C. The actual preservation
requirements depend on the target analyte(s), the analytical method that will be
used, and the data requirements for the contamination incident. Sampling personnel
should consult the site- and incident-specific documentation and/or receiving
laboratory for confirmation.

Sample Size - The amount of sample that should be collected for a minimum of one
analysis. The actual volume and weight requirements depend on the target
analyte(s), the analytical method that will be used, and the data requirements for the
contamination incident. Sampling personnel should consult the site- and incident-
specific documentation and/or receiving laboratory for confirmation.

Sample Type - The type of material to be collected for analysis (e.g., air, drinking
water, non-drinking water, solid, soil and sediment, wipe, vegetation). In cases where
the same analytical method(s) is indicated for analysis of both drinking and non-
drinking water sample types, the sample types and corresponding sample collection
information are combined into a single row.

Shipping Label - U.S. DOT shipping label requirements under 49 CFR 172 and
173.

7.0 Bibliography

SAM 2022 methods listed in Attachments A, B1, B2, C and D can be accessed through
the EPA Homeland Security Research Program's Sampling and Analytical Methods
website at https://www.epa.gov/esam/selected-analvtical-methods-environmental-
remediation-and-recoverv-sam (accessed April 2023). In addition to these methods, the
following resources were used to prepare this document:

U.S. Centers for Disease Control and Prevention. December 2020. Biosafetyin
Microbiological and Biomedical Laboratories, 6th Edition.
https://www.cdc.gov/labs/BMBL.html (accessed April 2023).

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U.S. Department of Energy. March 1979. "Radiochemical Analytical Procedures for
Analysis of Environmental Samples." Environmental Monitoring and Support
Laboratory. EMSL-LV-0539-17.

https://nepis.epa.gov/Exe/tiff2pnq.cqi/9101 RM32.PNG?-r+75+-
a+7+D%3A%5CZYFILES%5CINDEX%20DATA%5C76THRU80%5CTIFF%5C00005
005%5C9101 RM32.TIF (accessed April 2023).

U.S. Department of Labor. 29 CFR 1910.120, "Hazardous Waste Operations and
Emergency Response"; 29 CFR 1910.120, Appendix B, "General Description and
Discussion of the Levels of Protection and Protective Gear"; and 1910.38,
"Emergency Action Plans." http://www.osha.qov/laws-
regs/regulations/standardnumber/1910 (accessed April 2023).

U.S. Department of Labor. 29 CFR 1926.65, "Hazardous Waste Operations and
Emergency Response." http://www.osha.gov/laws-
regs/regulations/standardnumber/1926 (accessed April 2023).

U.S. Department of Transportation. 49 CFR 172.101. "Purpose and Use of
Hazardous Materials Table." http://www.ecfr.gov/cgi-bin/text-
idx?tpl=/ecfrbrowse/Title49/49cfr172 main 02.tpl (accessed April 2023).

U.S. Department of Transportation. 49 CFR 172.301, "General Marking
Requirements for Non-bulk Packagings." https://www.ecfr.gov/current/title-
49/subtitle-B/chapter-l/subchapter-C/part-172/subpart-D/section-172.301 (accessed
April 2023).

U.S. Department of Transportation. 49 CFR 173, "Shippers - General Requirements
for Shipments and Packaging." https://www.ecfr.gov/current/title-49/subtitle-
B/chapter-l/subchapter-C/part-173 (accessed April 2023).

-	49 CFR 173.132, "Class 6, Division 6.1-Definitions."
http://www.ecfr.gov/current/title-49/subtitle-B/chapter-l/subchapter-C/part-
173/subpart-D/section-173.132 (accessed April 2023).

-	49 CFR 173.150, "Exceptions for Class 3 (flammable and combustible liquids)."
http://www.ecfr.gov/current/title-49/subtitle-B/chapter-l/subchapter-C/part-173/
subpart-D/section-173.150 (accessed April 2023).

-	49 CFR 173.153, "Exceptions for Division 6.1 (poisonous materials)."
http://www.ecfr.gov/current/title-49/subtitle-B/chapter-l/subchapter-C/part-173/
subpart-D/section-173.153(accessed April 2023).

-	49 CFR 173.154, "Exceptions for Class 8 (corrosive materials)."
http://www.ecfr.gov/current/title-49/subtitle-B/chapter-l/subchapter-C/part-173/
subpart-D/section-173.154 (accessed April 2023).

-	49 CFR 173.24 and 173.24a, "General Requirements for Packagings and
Packages" and "Additional General Requirements for Non-bulk Packagings and
Packages." http://www.ecfr.gov/current/title-49/subtitle-B/chapter-l/subchapter-
C/part-173/subpart-D/section-173.24 (accessed April 2023) and
http://www.ecfr.gov/current/title-49/subtitle-B/chapter-l/subchapter-C/part-173/
subpart-D/section-173.24a (accessed April 2023).

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-	49 CFR 173.403, "Definitions." http://www.ecfr.qov/current/titl e-49/s u bti tl e-
B/chapter-l/subchapter-C/part-173/subpart-D/section-173.403 (accessed April
2023).

-	49 CFR 173.453, "Fissile Materials - Exceptions."
http://www.ecfr.gov/current/title-49/subtitle-B/chapter-l/subchapter-C/part-
173/subpart-D/section-173.453 (accessed April 2023).

U.S. Environmental Protection Agency. 40 CFR 61, Appendix B. "Standards of
Performance for New Stationary Sources, Performance Specifications - Test
Methods." https://www.ecfr.gov/current/title-40/chapter-l/subchapter-C/part-
61/appendix-Appendix%20B%20to%20Part%2061 (accessed April 2023)

U.S. Environmental Protection Agency. 2020. "Sample Collection Procedures for
Radiochemical Analytes in Environmental Matrices." EPA/600/R-20/247, National
Homeland Security Research Center, Cincinnati, OH.

https://cfpub.epa.gov/si/si public record report.cfm?dirEntrvld=350579&Lab=CESE
R&simplesearch=0&showcriteria=2&sortbv=pubDate&searchall=radiochemical&timst
ype=Published+Report&datebeginpublishedpresented=02/01/2019 (accessed April
2023).

U.S. Environmental Protection Agency. September 2016. "Sample Collection
Procedures for Radiochemical Analytes in Outdoor Building and Infrastructure
Materials." EPA/600/R-16/128, National Homeland Security Research Center,
Cincinnati, OH.

https://cfpub.epa.gov/si/si public record report.cfm?dirEntrvld=335065 (accessed
April 2023).

U.S. Environmental Protection Agency. Office of Solid Waste and Emergency
Response (OSWER). Hazardous Waste Test Methods / SW-846.
https://www.epa.gov/hw-sw846 (accessed April 2023).

U.S. Environmental Protection Agency. Office of Water. February 2017. Sampling
Guidance for Unknown Contaminants in Drinking Water. EPA-817-R-08-003.
https://www.epa.gov/waterlabnetwork/sampling-guidance-unknown-contaminants-
drinking-water (accessed April 2023)

U.S. Office of Management and Budget. January 27, 2016. 81 Federal Register
4673: Revisions of OMB Circular No. A-119 "Federal Participation in the
Development and Use of Voluntary Consensus Standards and in Conformity
Assessment Activities." https://www.gpo.gov/fdsys/pkg/FR-2016-01-27/pdf/2016-
01606.pdf (accessed April 2023).

U.S. Environmental Protection Agency. 2022. Selected Analytical Methods for
Environmental Remediation and Recovery (SAM) 2022. EPA/600/R-21/320, National
Homeland Security Research Center, Cincinnati, OH.

https://www.epa.gov/esam/selected-analvtical-methods-environmental-remediation-
and-recoverv-sam (accessed April 2023).

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Attachment A

Sample Collection Information for the
Chemical Analytes and Methods Listed in

SAM 2022

[Note: The acronyms and abbreviations used in this
attachment are listed and defined in the beginning of the

report.]

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Sample Collection Information Document - Attachment A

April 2023

Attachment A: Sample Collection Information for the Chemical Analytes and Methods Listed in SAM 2022

Note: In some cases, analytes are listed as not a concern in a particular sample type; in these cases, SAM work groups have determined that the analyte is not a concern due to a number of factors, including the analyte's low likelihood of persistence, toxicity, mobility or solubility within the particular
sample type.

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

A-230

(Methyl-(1-(diethylamino) ethylidene)phosphonamido-
fluoridate)

A-232

(Methyl-(1-(diethylamino) ethylidene)phosphoramido-
fluoridate)

A-234

(Ethyl N-[(1E)-1-(diethylamino) ethylidene]-phosphoramido-
fluoridate)

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach
wipe, taking care not to
obscure labeling.

TO-17 (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Until holding time data
are available, analyze
immediately/ as soon as
possible

•	Cool to < 6°C; keep from
freezing

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

SOP L-P-107, Rev. 3

(EPA PHI LIS) /
SOP L-A-507, Rev. 3

(EPA PH I LIS)

[Tier II]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Acephate

Air

TBD

TBD

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C

Wrap collection vessel in aluminum foil,
and place in a clean sealed container.
Place container in double plastic bags and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

J. Chromatogr. A (2007)
1154(1): 3-25
[Tier III]

Drinking Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Prior to collection, add 400
|jL of ammonium acetate
concentrated stock and 80 n L
of concentrated sodium
omadine stock to sample
container [Concentrations of
ammonium acetate and
sodium omadine in the sample
should be 1.5 g/L and 64
mg/L, respectively.]

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

538 (EPA OW)
[Tier I]

Non-Drinking
Water

538 (EPA OW)
[Tier II]

Solid

100 mL

Wide-mouth glass or
fluoropolymer container with
fluoropolymer-lined lid

Until holding time data
are available, analyze
immediately/ as soon as
possible

•	Cool to < 6°C

•	Protect from light

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

J. Env. Sci. Health (2014) 49:
23-34 /

538 (EPA OW)

[Tier II]

Wipes

1 wipe/100 cm or
1 wipe/ft2

J. Chromatogr. A (2007)
1154(1): 3-25

[Tier III]

SAM Companion - Sample Collection Information Document

A1-1

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Acrylamide

Air

120 L (flow rate = 1
L/min)

Filter/solid sorbent tube
(13mm quartz fiber filter)

•	OVS-7 tube (XAD-7 resin)

•	OVS-2 (XAD-2 resin)

•	OVS-SG (silica gel)

13 days

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Acrylamide, solid, 6.1,
Poison, UN2074

PV2004 (OSHA)
[Tier 1]

Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the bottle with
non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Acrylamide solution,
6.1, Poison, UN3426

8316 (EPA SW-846)
[Tier II]

Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septa or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Acrylamide, solid, 6.1,
Poison, UN2074

Water Extraction /
8316 (EPA SW-846)
[Tier III]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

3570 / 8290A Appendix A /
8316 (EPA SW-846)

[Tier III]

Acrylonitrile

Air

120 L (flow rate = 1
L/min)

Filter/solid sorbent tube
(13mm quartz fiber filter)

•	OVS-7 tube (XAD-7 resin)

•	OVS-2 (XAD-2 resin)

•	OVS-SG (silica gel)

13 days

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Acrylonitrile, stabilized,
3, Flammable liquid,
6.1, Poison, UN1093

PV2004 (OSHA)
[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 24 hours if
not acidified

Prior to collection of treated
water samples, add 25 mg
ascorbic acid to container for
removal of residual chlorine

Method 524.2:

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

524.2/524.3/524.4

(EPA OW)

[Tier II]

• Adjust to pH < 2 with 1:1
HCI

Methods 524.3 and 524.4:

• Adjust to pH < 2 with maleic
acid

For all samoles:

•	If residual chlorine is > 5
mg/L, add an additional 25 mg
ascorbic acid per each 5 mg/L
chlorine

•	Cool to < 6°C; keep from
freezing

Solid

5-g coring device or
amber VOA vial

AND

1 - 2 oz. glass jar
(if needed to
determine percent
moisture)

Coring device (If coring device
is to be cleaned and reused,
samples can be transferred to
an amber VOA vial.)

AND glass jar (if needed to
determine percent moisture)

14 days if frozen; 48
hours if not frozen

Cool to < 6°C; freeze within 48
hours to < negative (-) 7°C
[Coring tools should not be
frozen below -20"C.]

Wipe outside of coring device or container
clean using a damp, then dry cloth. Place
the coring device or container in a zipper
bag. Pack samples as described in
Footnote (4).

5035A / 8260D

(EPA SW-846)
[Tier II]

Wipes

1 wipe/100 cm or
1 wipe/ft

Screw-cap VOA vial with
fluoropolymer-lined septum or
lid

14 days

•	Cool to < 6°C

•	Protect from light

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

3570 / 8290A Appendix A /
8260D (EPA SW-846)

[Tier III]

SAM Companion - Sample Collection Information Document

A1-2

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Aldicarb (Temik)

Air

240 - 480 L
(flow rate = 0.1-1
L/min)

Filter/solid sorbent tube (OVS-
2 tube: 13 mm quartz fiber
filter, XAD-2 [270 mg/140 mg])

30 days if frozen; 7 days
at room temperature
(24°C)

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757

5601 (NIOSH)
[Tier I]



Drinking Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

28 days

•	Prior to collection, add 0.4 g
potassium dihydrogen citrate
and 3.2 - 4.8 mg sodium
thiosulfate to sample container

•	Agitate for 1 minute after
collection

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Carbamate pesticides,
liquid, toxic, 6.1,
Poison, UN2992

531.2 (EPA OW)
[Tier I]



Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Prior to collection of treated
water samples, add 10 mg
ascorbic acid to sample
container

•	Acidify to pH < 3.8 with
glacial acetic acid

•	Cool to < 6°C; keep from
freezing





D7645-16 (ASTM)
[Tier II]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or

7 days

Cool to < 6°C



Standard carrier
shipping label AND
Carbamate pesticides,

8318A (EPA SW-846)
[Tier II]



Wipes

1 wipe/100 cm2or
1 wipe/ft2

lid







solid, toxic, 6.1,
UN2757

3570 / 8290A Appendix A /
8318A (EPA SW-846)

[Tier III]

Aldicarb sulfone

Air

240 - 480 L
(flow rate = 0.1-1
L/min)

Filter/solid sorbent tube (OVS-
2 tube: 13 mm quartz filter,
XAD-2, [270 mg/140 mg])

30 days if frozen; 7 days
at room temperature
(24°C)

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757

5601 (NIOSH)
[Tier III]



Drinking Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

28 days

•	Prior to collection, add 0.4 g
potassium dihydrogen citrate
and 3.2 - 4.8 mg sodium
thiosulfate to sample container

•	Agitate for 1 minute after
collection

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Carbamate pesticides,
liquid, toxic, 6.1,
Poison, UN2992

531.2 (EPA OW)
[Tier I]



Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Prior to collection of treated
water samples, add 10 mg
ascorbic acid to sample
container

•	Acidify to pH < 3.8 with
glacial acetic acid

•	Cool to < 6°C; keep from
freezing





D7645-16 (ASTM)
[Tier II]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or

7 days

Cool to < 6°C



Standard carrier
shipping label AND
Carbamate pesticides,

8318A (EPA SW-846)
[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

lid







solid, toxic, 6.1,
UN2757

3570 / 8290A Appendix A /
8318A (EPA SW-846)

[Tier III]

SAM Companion - Sample Collection Information Document

A1-3

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Aldicarb sulfoxide

Air

240 - 480 L
(flow rate = 0.1-1
L/min)

Filter/solid sorbent tube (OVS-
2 tube: 13 mm quartz filter,
XAD-2, [270 mg/140 mg])

30 days if frozen; 7 days
at room temperature
(24°C)

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757

5601 (NIOSH)
[Tier III]



Drinking Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

28 days

•	Prior to sample collection,
add 0.4 g potassium
dihydrogen citrate and 3.2 -
4.8 mg sodium thiosulfate to
sample container

•	Agitate for 1 minute after
collection

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Carbamate pesticides,
liquid, toxic, 6.1,
Poison, UN2992

531.2 (EPA OW)
[Tier I]



Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Prior to collection of treated
water samples, add 10 mg
ascorbic acid to sample
container

•	Acidify to pH < 3.8 with
glacial acetic acid

•	Cool to < 6°C; keep from
freezing





D7645-16 (ASTM)
[Tier II]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or

7 days

Cool to < 6°C



Standard carrier
shipping label AND
Carbamate pesticides,

8318A (EPA SW-846)
[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

lid







solid, toxic, 6.1,
UN2757

3570 / 8290A Appendix A /
8318A (EPA SW-846)

[Tier III]

Allyl alcohol

Air

1-L or 6-L canister
(depending on
suspected
concentration)1 1

Specially prepared stainless
steel canister

For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the
sample is collected using a
pump and flow control
arrangement to achieve a
typical 101 - 202 kPa (15 - 30

psig)

30 days

None

Ship canister in container provided by
laboratory

Standard carrier
shipping label AND Allyl
alcohol, 3, Flammable
liquid, 6.1, Poison,
UN1098

TQ-15 (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 7 days if
not acidified

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Adjust to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap glass bottles with bubble
wrap. Pack samples as described in
Footnote (4).



5030C / 8260D

(EPA SW-846)
[Tier II]



Solid

5-g coring device or
amber VOA vial

AND

1 - 2 oz. glass jar (if
needed to determine
percent moisture)

Coring device (If coring device
is to be cleaned and reused,
samples can be transferred to
an amber VOA vial.)

AND glass jar (if needed to
determine percent moisture)

14 days if frozen; 48
hours if not frozen

Cool to < 6°C; freeze within 48
hours to < negative (-) 7°C
[Coring tools should not be
frozen below -20 °C.]

Wipe outside of coring device or container
clean using a damp, then dry cloth. Place
the coring device or container in a zipper
bag. Pack samples as described in
Footnote (4).



5035A / 8260D

(EPA SW-846)
[Tier II]



Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

SAM Companion - Sample Collection Information Document

A1-4

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

4-Aminopyridine

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Aminopyridines, 6.1,
Poison, UN2671

3535A / 8330B

(EPA SW-846)
[Tier III]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C





8330B (EPA SW-846)
[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days







3570 / 8290A Appendix A /
8330B

(EPA SW-846)

[Tier III]

Ammonia

Air

0.1 (at 50 ppm) - 96
L (flow rate = 0.1 -
0.2 L/min)

Solid sorbenttube (sulfuric
acid-treated silica gel)

35 days (at 5°C)

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap tube with plastic
(not rubber) caps immediately after
sampling. Place tubes in double plastic
bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND (if
greater than 10%
ammonia by volume)
Ammonia solution, 8,
Corrosive, UN2672

6016 (NIOSH)
[Tier I]



Drinking Water

500 mL

Polyethylene or glass
container

28 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples (1 (Can
be added in the field or upon
receipt in the laboratory.)
•Adjust to pH < 2 with H2S04

•	Cool to< 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



350.1 (EPA OW)
[Tier I]



Non-Drinking
Water

500 mL

Polyethylene or glass
container

28 days with
acidification; analyze
immediately / as soon as
possible if not acidified

•	Cool to < 6°C or acidify to pH
< 2; keep from freezing

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples (5) (Can
be added in the field or upon
receipt in the laboratory.)





4500-NH3 B/
4500- NH3 G (SM)
[Tier I]



Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Ammonium metavanadate
(analyze as total vanadium)

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Ammonium
metavanadate, 6.1,
Poison, UN2859

IO-3.1 /
10-3.4/10-3.5

(EPA ORD)
[Tier 1]



Drinking Water

125 mL

Fluoropolymer, plastic or glass
container

6 months with
acidification. Samples
must be acidified within
14 days of collection.

•	Acidify to pH < 2 with

hno3(7)

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



200.7/200.8 (EPA OW)
[Tier 1]



Non-Drinking
Water











3015A /

6010D /6020B (EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

Analyze immediately / as
soon as possible

Cool to < 6°C





3050B / 3051A /

6010D /6020B (EPA SW-846)

[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











9102 (NIOSH)/

6010D /6020B (EPA SW-846)

[Tier I]

SAM Companion - Sample Collection Information Document

A1-5

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Arsenic trioxide
(analyze as total arsenic)

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Arsenic trioxide, 6.1,
Poison, UN1561

IO-3.11
10-3.4/10-3.5

(EPA ORD)
[Tier 1]



Drinking Water

125 mL

Fluoropolymer, plastic or glass
container

6 months with
acidification. Samples
must be acidified within
14 days of collection.

• Acidify to pH < 2 with
HN03(7> [If arsenic species are

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



200.7/200.8 (EPA OW)
[Tier 1]



Non-Drinking
Water





to be determined, remove
unpreserved aliquot prior to
sample preservation.]

• Cool to < 6°C; keep from
freezing



3015A /

6010D /6020B (EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

Analyze immediately/ as
soon as possible

Cool to < 6°C





3050B / 3051 A/

6010D /6020B (EPA SW-846)

[Tier I]



Wipes

1 wipe/100 cm or
1 wipe/ft











9102 (NIOSH)/

6010D /6020B (EPA SW-846)

[Tier I]

Arsenic, Total

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Arsenic, 6.1, Poison,
UN1558

IO-3.1 /
10-3.4/10-3.5

(EPA ORD)
[Tier 1]



Drinking Water

125 mL

Fluoropolymer, plastic or glass
container

6 months with
acidification. Samples
must be acidified within
14 days of collection.

•	Acidify to pH < 2 with
HN03(7) [If arsenic species are
to be determined, remove
unpreserved aliquot prior to
sample preservation.]

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass



200.7/200.8 (EPA OW)
[Tier 1]



Non-Drinking
Water







containers with bubble wrap. Pack
samples as described in Footnote (4).



3015A /

6010D /6020B (EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

Analyze immediately/ as
soon as possible

Cool to < 6°C





3050B / 3051A /

6010D /6020B (EPA SW-846)

[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











9102 (NIOSH)/

6010D /6020B (EPA SW-846)

[Tier I]

Arsine

(analyze as total arsenic in
non-air samples)

Air

0.1 L (at 0.05 ppm)
- 10 L (flow rate =
0.01 -0.2 L/min)

Solid sorbenttube (coconut
shell charcoal [100 mg/50 mg])

6 days

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap tubes with
plastic (not rubber) caps. Place tubes in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Arsine, 2.1, Flammable
gas, 2.3, Poisonous
gas, UN2188

6001 (NIOSH)
[Tier I]



Drinking Water

125 mL

Fluoropolymer, plastic or glass
container

6 months with
acidification. Samples
must be acidified within
14 days of collection.

•	Acidify to pH < 2 with
HN03(7> [If arsenic species are
to be determined, remove
unpreserved aliquot prior to
sample preservation.]

•	Acidified samples can be
cooled to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape orfilm (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack



200.7/200.8 (EPA OW)
[Tier I]



Non-Drinking
Water







samples as described in Footnote (4).



3015A /

6010D /6020B (EPA SW-846)
[Tier I]



Solid

40 mL

Wide-mouth fluoropolymer,
plastic or glass container

Analyze immediately/ as
soon as possible

Cool to < 6°C





3050B / 3051A /

6010D /6020B (EPA SW-846)

[Tier I]



Wipes

1 wipe/100 cm or
1 wipe/ft











9102 (NIOSH)/

6010D /6020B (EPA SW-846)

[Tier I]

SAM Companion - Sample Collection Information Document

A1-6

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Asbestos

Air

500 - 5000 L
(flow rate = 1-10
L/min)

Polycarbonate or cellulose
ester filter

Samples can be held
indefinitely

None

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Asbestos, 9, Class 9,
NA2212

10312:1995 (ISO)
[Tier I]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

200 mL

Wide-mouth, sealable glass or
fluoropolymer container

Samples can be held
indefinitely

Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Asbestos, 9, Class 9,
NA2212

D5755-09 (e1)

(soft surfaces-microvac)

(ASTM)

[Tier III]

Wipes

1 particle-free
wipe/100 cm2

Wide-mouth, sealable glass or
fluoropolymer container

Samples can be held
indefinitely

Cool to < 6°C

D6480-19 (ASTM)
[Tier I]

Boron trifluoride

Air

Up to 480 L
(flow rate = 1 L/min)

Midget glass bubbler
containing 10 mLofO.1 M
NH4F; sample solution
transferred to 20-m L glass vial
with fluoropolymer-lined cap

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based). Wrap glass vials with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Boron trifluoride, 2.3,
Poisonous gas,
UN1008

ID216SG (OSHA)
[Tier I]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Brodifacoum

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Add 10 mg of dry ascorbic
acid to each 40 m L vial prior to
collection

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Coumarin derivative
pesticides, liquid, toxic,
6.1, Poison, UN3023

D7644-16 (ASTM)
[Tier II]

Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid, toxic,
6.1, Poison, UN3026

3541 / 3545A (EPA SW-846)/
D7644-16 (ASTM)

[Tier III]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

3570 / 8290A Appendix A

(EPA SW-846)/

D7644-16 (ASTM)

[Tier III]

Bromadiolone

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Add 10 mg of dry ascorbic
acid to each 40 m L vial prior to
collection

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Coumarin derivative
pesticides, liquid, toxic,
6.1, Poison, UN3023

D7644-16 (ASTM)
[Tier II]

Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid, toxic,
6.1, Poison, UN3026

3541 / 3545A (EPA SW-846)/
D7644-16 (ASTM)

[Tier III]

Wipes

1 wipe/100 cm2or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

3570 / 8290A Appendix A

(EPA SW-846)/

D7644-16 (ASTM)

[Tier III]

SAM Companion - Sample Collection Information Document

A1-7

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

[BZ] Quinuclidinyl benzilate

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

Analyze as soon as
possible until holding
time are available

Cool to < 6°C

Wrap cartridge in aluminum foil, and place
in a clean sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach

TO-10A (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Analyze as soon as
possible until holding
time data are available

Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon

wipe, taking care not to
obscure labeling.

J. Chromatogr. B (2008) 874:
42-50

[Tier III]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Analyze as soon as
possible until holding
time data are available

Cool to < 6°C

plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.



3541/3545A (EPA SW-846) /
J. Chromatogr. B (2008) 874:
42-50

[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











3570/8290A Appendix A
(EPA SW-846) /
J. Chromatogr. B (2008) 874:
42-50

[Tier III]

Calcium arsenate
(analyze as total arsenic)

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Calcium arsenate, 6.1,
Poison, UN1573

IO-3.1 /
10-3.4/10-3.5

(EPA ORD)
[Tier 1]



Drinking Water

125 mL

Fluoropolymer, plastic or glass
container

6 months with
acidification. Samples
must be acidified within
14 days of collection.

•	Acidify to pH < 2 with
HN03(7> [If arsenic species are
to be determined, remove
unpreserved aliquot prior to
sample preservation.]

•	Acidified samples can be
cooled to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



200.7/200.8 (EPA OW)
[Tier 1]



Non-Drinking
Water









3015A /

6010D /6020B (EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

Analyze immediately/ as
soon as possible

Cool to < 6°C





3050B / 3051A /

6010D /6020B (EPA SW-846)

[Tier I]



Wipes

1 wipe/100 cm or
1 wipe/ft











9102 (NIOSH)/

6010D /6020B (EPA SW-846)

[Tier I]

SAM Companion - Sample Collection Information Document

A1-8

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Carbofuran (Furadan)

Air

240 - 480 L
(flow rate = 0.1-1
L/min)

Filter/solid sorbent tube (OVS-
2 tube: 13 mm quartz fiber
filter, XAD-2 [270 mg/140 mg])

30 days if frozen; 7 days
at room temperature
(24°C)

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757

5601 (NIOSH)
[Tier I]

Drinking Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

28 days

•	Prior to collection, add 0.4 g
potassium dihydrogen citrate
and 3.2 - 4.8 mg sodium
thiosulfate to sample container

•	Agitate for 1 minute after
collection

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Carbamate pesticides,
liquid, toxic, 6.1,
Poison, UN2992

531.2 (EPA OW)
[Tier I]

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Prior to collection of treated
water samples, add 10 mg
ascorbic acid to sample
container

•	Acidify to pH < 3.8 with
glacial acetic acid

•	Cool to < 6°C; keep from
freezing

D7645-16 (ASTM)
[Tier II]

Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

Cool to < 6°C

Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757

8318A (EPA SW-846)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

3570 / 8290A Appendix A /
8318A (EPA SW-846)

[Tier III]

SAM Companion - Sample Collection Information Document

A1-9

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Carbon disulfide

Air

1-L or 6-L canister
(depending on
suspected
concentration)* 1

Specially prepared stainless
steel canister

For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the
sample is collected using a
pump and flow control
arrangement to achieve a
typical 101 - 202 kPa (15 - 30

psig)

30 days

None

Ship canister in container provided by
laboratory

Standard carrier
shipping label AND
Carbon disulfide, 3,
Flammable liquid, 6.1,
Poison, UN1131

TQ-15 (EPA ORD)
[Tier I]



Drinking Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 24 hours if
not acidified

Prior to collection of treated
water samples, add 25 mg
ascorbic acid to container for
removal of residual chlorine

Method 524.2:

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



524.2/524.3/524.4

(EPA OW)

[Tier I]











• Adjust to pH < 2 with 1:1
HCI















Methods 524.3 and 524.4;

















• Adjust to pH < 2 with maleic
acid

















For all samoles:

















•	If residual chlorine is > 5
mg/L, add an additional 25 mg
ascorbic acid per each 5 mg/L
chlorine

•	Cool to < 6°C; keep from
freezing









Non-Drinking
Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 7 days if
not acidified

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'5'

•	Adjust to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).



5030C / 8260D

(EPA SW-846)
[Tier I]



Solid

5-g coring device or
amber VOA vial

AND

1 -2 oz. glass jar
(if needed to
determine percent
moisture)

Coring device (If coring device
is to be cleaned and reused,
samples can be transferred to
an amber VOA vial.)

AND glass jar (if needed to
determine percent moisture)

14 days iffrozen; 48
hours if not frozen

Cool to < 6°C; freeze within 48
hours to < negative (-) 7°C
[Coring tools should not be
frozen below -20°C.]

Wipe outside of coring device or container
clean using a damp, then dry cloth. Place
the coring device or container in a zipper
bag. Pack samples as described in
Footnote (4).



5035A / 8260D

(EPA SW-846)
[Tier I]



Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Carfentanil

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organic, n.o.s., 6.1,
toxic, UN2810

3520C /3535A (EPA SW-846) /
J. Chromatogr. B (2014) 962:
52-58

[Tier III]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C



Standard carrier
shipping label AND
Organic, n.o.s., solid,
6.1, Poison, UN2811

3541 /3545A (EPA SW-846)
J. Chromatogr. B (2014) 962:
52-58

[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Glass container with
fluoropolymer-lined lid

7 days

•	Cool to < 6°C

•	Protect from light





SOP L-A-309 Rev.O/
SOP L-A-310 Rev.1

(EPA PH I LIS)

[Tier II]

SAM Companion - Sample Collection Information Document

A1-10

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Chlorfenvinphos

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier II]



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

3520C / 3535A / 8270E

(EPA SW-846)

[Tier I]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus

EPA/600/R-16/114 (EPA)
[Tier II]



Wipes

1 wipe/100 cm or
1 wipe/ft







pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]

Chlorine

Air

8 L (at 0.1 ppm) -
360 L (flow rate =
0.039-2.94 L/min)

Prefilter and filter
(fluoropolymer, 0.5 |jm and
silver membrane, 25 mm, 0.45
Mm)

30 days

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Chlorine, 2.3, 5.1,
Oxidizer, 8, Poison gas,
UN1017

Analyst (1999) 124(12): 1853-
1857/

4500-CI G (SM)

[Tier II]



Drinking Water

Non-Drinking
Water

500 mL

Amber glass or fluoropolymer
container

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C

Avoid excessive agitation

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



4500-CI G (SM)
[Tier I]



Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

2-Chloroethanol

Air

2 (at 5 ppm) - 35 L
(flow rate = 0.01 -
0.2 L/min)

Solid sorbent tube (petroleum
charcoal [100 mg/50 mg])

14 days

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap tubes with
plastic (not rubber) caps. Place tubes in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Ethylene chlorohydrin,
3, Flammable liquid,
6.1, Poison, UN1135

2513 (NIOSH)
[Tier I]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 7 days if
not acidified

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples.(5>

•	Adjust to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).



5030C / 8260D

(EPA SW-846)
[Tier II]



Solid

5-g coring device or
amber VOA vial

AND

1 - 2 oz. glass jar
(if needed to
determine percent
moisture)

Coring device (If coring device
is to be cleaned and reused,
samples can be transferred to
an amber VOA vial.)

AND glass jar (if needed to
determine percent moisture)

14 days if frozen; 48
hours if not frozen

Cool to < 6°C; freeze within 48
hours to < negative (-) 7°C
[Coring tools should not be
frozen below-20°C.]

Wipe outside of coring device or container
clean using a damp, then dry cloth. Place
the coring device or container in a zipper
bag. Pack samples as described in
Footnote (4).



5035A / 8260D

(EPA SW-846)
[Tier II]



Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

SAM Companion - Sample Collection Information Document

A1-11

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Chloropicrin

Air

5 L (flow rate = 0.2
L/min)

Solid sorbenttube (XAD-4
[100 mg/50 mg])

Until holding time data
are available, analyze
immediately/ as soon as
possible

•	Cool to < 6°C

•	Protect from light

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap tubes with
plastic (not rubber) caps. Place tubes in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Chloropicrin, 6.1,
Poison, UN 1580

PV2103 (OSHA)
[Tier I]



Drinking Water

Non-Drinking
Water

60 mL

Amber glass vial with
fluoropolymer-lined septum

14 days

•	Prior to collection of treated
water samples, add 6 mg
NH4CI to sample container

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a bleach
wipe, taking care not to affect any labeling.
Make sure bleach is removed and the
outside of the vial is dry, then seal the vial
with non-reactive tape or film (e.g.,
fluoropolymer-based), and wrap with



551.1 (EPA OW)
[Tier I]



Solid

40 mL

Amber glass vial with
fluoropolymer-lined septum

14 days

Cool to < 6°C

in Footnote (4).



EPA/600/R-16/114 (EPA)
[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











EPA/600/R-16/114 (EPA)
[Tier II]

3-Chloro-1,2-propanediol

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Glycerol alpha-
monochlorohydrin, 6.1,
Toxic substance,
UN2689

TO-10A (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

15 days

•	Adjust pH to 3 - 8 with
sodium sulfite or 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).



J. Chromatogr. A (2000) 866:
65-77

[Tier II]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



Eur. J. Lipid Sci. Technol.
(2011) 113: 345-355

[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2







Eur. J. Lipid Sci. Technol.
(2011)113:345-355

[Tier III]

Chlorosarin

Air

1 -4 L

(flow rate = 16 - 67
mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach
wipe, taking care not to
obscure labeling.

TO-17 (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Cool to < 6°C; keep from
freezing

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before

EPA/600/R-16/115 (EPA)
[Tier III]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

placing in cooler.



EPA/600/R-16/115 (EPA)
[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











EPA/600/R-16/115 (EPA)
[Tier III]

SAM Companion - Sample Collection Information Document

A1-12

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Chlorosoman

Air

1 -4 L

(flow rate = 16 - 67
mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach
wipe, taking care not to
obscure labeling.

TQ-17 (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass orfluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

EPA/600/R-16/115 (EPA)
[Tier III]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

EPA/600/R-16/115 (EPA)
[Tier III]

Wipes

1 wipe/100 cm or
1 wipe/ft2

EPA/600/R-16/115 (EPA)
[Tier III]

2-Chlorovinylarsonic acid (CVAOA) (degradation product of
Lewisite)

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach
wipe, taking care not to
obscure labeling.

IO-3.1

IO-3.4/IO-3.5

(EPA ORD)
[Tier I]

Drinking Water

Non-Drinking
Water

40 mL

VOA vial with fluoropolymer-
lined cap

14 days

Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a bleach
wipe, taking care not to affect any labeling.
Make sure bleach is removed and the
outside of vial is dry, then wrap it with
bubble wrap. Place in gallon plastic
(polypropylene or polyethylene) bag with
zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

EPA/600/R-15/258 (EPA)
[Tier II]

Solid

40 mL

VOA vial with fluoropolymer-
lined cap

Analyze immediately/ as
soon as possible

Cool to < 6°C

EPA/600/R-15/258 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

VOA vial with fluoropolymer-
lined cap

14 days

EPA/600/R-15/258 (EPA)
[Tier II]

2-Chlorovinylarsonous acid (CVAA) (degradation product of
Lewisite)

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach
wipe, taking care not to
obscure labeling.

IO-3.1

IO-3.4/IO-3.5

(EPA ORD)
[Tier I]

Drinking Water

Non-Drinking
Water

40 mL

VOA vial with fluoropolymer-
lined cap

14 days

Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a bleach
wipe, taking care not to affect any labeling.
Make sure bleach is removed and the
outside of vial is dry, then wrap it with
bubble wrap. Place in gallon plastic
(polypropylene or polyethylene) bag with
zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

EPA/600/R-15/258 (EPA)
[Tier II]

Solid

40 mL

VOA vial with fluoropolymer-
lined cap

Analyze immediately/ as
soon as possible

Cool to < 6°C

EPA/600/R-15/258 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

VOA vial with fluoropolymer-
lined cap

14 days

EPA/600/R-15/258 (EPA)
[Tier II]

SAM Companion - Sample Collection Information Document

A1-13

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Chlorpyrifos

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier I]



Drinking Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Method 525.2:

•	Add sodium sulfite to empty
sample container to remove
residual chlorine from treated
water samples'51

•	Acidify to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Method 525.3 (to emDtv
sample container):

•	Add 4 mg ascorbic acid to
remove residual chlorine from
treated water samples

•	Add 14 mg trisodium EDTA,
and 400 mg potassium
dihydrogen citrate

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

525.2/525.3 (EPA OW)
[Tier I]



Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

For treated water samDles:
Preservatives/dechlorinating
agents not required, but the
analyte is not affected by the
following, if added to address
other analytes:

•	1.6-2.0 mg NH4CI

For all other samnles-

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a bleach
wipe, taking care not to affect any labeling.
Make sure bleach is removed and the
outside of the vial is dry, then seal the vial
with non-reactive tape or film (e.g.,
fluoropolymer-based), and wrap with
bubble wrap. Pack samples as described
in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

EPA/600/R-16/114 (EPA)
[Tier II]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C



Standard carrier
shipping label AND
Organophosphorus

EPA/600/R-16/114 (EPA)
[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2









pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]

SAM Companion - Sample Collection Information Document

A1-14

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Chlorpyrifos oxon

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

250 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Prior to collection, add solid
ascorbic acid (dechlorinating
agent), Trizma® base
(buffering agent) and
chloroacetamide (antimicrobial
preservative) at
concentrations of 7.75 g/L, 2
g/L and 100 mg/L, respectively
to an empty sample container

•	After collecting the sample,
cap the container and agitate
until preservative is dissolved

•	Cool samples to < 10°C;
keep from freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

540 (EPA OW)
[Tier I]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier III]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier III]

Crimidine

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Preservatives/dechlorinating
agents not required, but the
analyte is not affected by the
following, if added to address
other analytes:

•	2.0 mg NH4CI

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of the vial is dry, then seal the
vial with non-reactive tape or film (e.g.,
fluoropolymer-based), and wrap with
bubble wrap. Pack samples as described
in Footnote (4).

Standard carrier
shipping label AND
Pesticide, n.o.s., liquid,
6.1, Poison, UN2902

EPA/600/R-16/114 (EPA)
[Tier II]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Pesticide, n.o.s., solid,
6.1, Poison, UN2588

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier II]

Cyanide, Amenable to chlorination

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Drinking Water

Non-Drinking
Water

1 L

Glass or polyethylene
container

14 days

For treated water samDles:

•	Add 0.6 g of ascorbic acid for
each liter of sample volume

For all samDles:

•	Adjust to pH > 12 with NaOH

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Cyanide solutions n.o.s,
6.1, Poison, UN1935

3135.21 (EPA Regional Lab)
[Tier 1]

Solid

250 mL

Wide-mouth glass or
polyethylene container

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Cyanide inorganic solid
n.o.s, 6.1, Poison,
UN1588

3135.21 (EPA Regional Lab)
[Tier 1]

Wipes

1 wipe/100 cm or
1 wipe/ft

3135.21 (EPA Regional Lab)
[Tier III]

SAM Companion - Sample Collection Information Document

A1-15

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Cyanide, Total

Air

2 (at 5 ppm) - 90 L
(flow rate = 0.05 -
0.2 L/min)

Solid sorbent tube (soda lime
[600 mg/200 mg])

14 days

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap tubes with
plastic (not rubber) caps. Place tubes in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Cyanides, inorganic,
solid, n.o.s, 6.1, Poison,
UN1588

6010 (NIOSH)
[Tier I]

Drinking Water

125 mL

Amber glass or polyethylene
container

14 days

•	Add 0.6 g of ascorbic acid for
each liter of sample volume

•	Adjust to pH > 12 with NaOH

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Cyanide solutions n.o.s,
6.1, Poison, UN1935

335.4 (EPA OW)
[Tier I]

Non-Drinking
Water

500 mL

Amber glass or polyethylene
container

14 days

For treated water samDles:

ISM 02.3 CN (EPA CLP)
[Tier I]

'• Add 0.6 g of ascorbic acid
for each liter of sample volume

For all samDles:

•	Adjust to pH > 12 with NaOH

•	Cool to < 6°C; keep from
freezing

Solid

40 mL

Wide-mouth amber glass or
polyethylene container

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Cyanides, inorganic,
solid, n.o.s, 6.1, Poison,
UN1588

ISM 02.3 CN (EPA CLP)
[Tier I]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

ISM 02.3 CN (EPA CLP)
[Tier III]

Cyanogen chloride

Air

1-L or 6-L canister
(depending on
suspected
concentration)' 1

Specially prepared stainless
steel canister

For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the
sample is collected using a
pump and flow control
arrangement to achieve a
typical 101 - 202 kPa (15 - 30

psig)

Analyze immediately/ as
soon as possible

None

Ship canister in container provided by
laboratory

Standard carrier
shipping label AND
Cyanogen chloride, 2.3,
Poisonous gas,

UN1589

TQ-15 (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated wastewater samples'5'

•	Adjust to pH < 2with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the container with non-
reactive tape or film (e.g., fluoropolymer-
based). Wrap glass containers with bubble
wrap. Pack samples as described in
Footnote (4).

Encyclopedia of Anal. Chem.
(2006) DOI:
10.1002/9780470027
318.a0809

[Tier II]

Solid

5-g coring device or
amber VOA vial

AND

1 - 2 oz. glass jar
(if needed to
determine percent
moisture)

Coring device (If coring device
is to be cleaned and reused,
samples can be transferred to
an amber VOA vial.)

AND glass jar (if needed to
determine percent moisture)

14 days if frozen; 48
hours if not frozen

Cool to < 6°C; freeze within 48
hours to < negative (-) 7°C
[Coring tools should not be
frozen below -20°C.]

Wipe outside of coring device or container
clean using a damp, then dry cloth. Place
the coring device or container in a zipper
bag. Pack samples as described in
Footnote (4).

Encyclopedia of Anal. Chem.
(2006) DOI:
10.1002/9780470027
318.a0809

[Tier II]

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

SAM Companion - Sample Collection Information Document

A1-16

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Cyclohexyl sarin (GF)

Air

1 -4 L

(flow rate = 16 - 67
mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch O.D.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach

TO-17 (EPA ORD)
[Tier II]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated wastewater samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag

wipe, taking care not to
obscure labeling.

EPA/600/R-16/115 (EPA)
[Tier I]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.



EPA/600/R-16/115 (EPA)
[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











EPA/600/R-16/115 (EPA)
[Tier I]

1,2-Dichloroethane
(degradation product of HD)

Air

1-L or 6-L canister
(depending on
suspected
concentration)'61

Specially prepared stainless
steel canister

For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the
sample is collected using a
pump and flow control
arrangement to achieve a
typical 101 -202 kPa (15-30

psig)

30 days

None

Ship canister in container provided by
laboratory

Standard carrier
shipping label AND
Ethylene dichloride, 3,
Flammable, 6.1,
Poison, UN1184

TQ-15 (EPA ORD)
[Tier I]



Drinking Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 24 hours if
not acidified

Prior to collection of treated
water samples, add 25 mg
ascorbic acid to container for
removal of residual chlorine

Method 524.2:

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



524.2/524.3/524.4

(EPA OW)

[Tier I]











• Adjust to pH < 2 with 1:1
HCI















Methods 524.3 and 524.4:

















• Adjust to pH < 2 with maleic
acid

















For all samoles:

















•	If residual chlorine is > 5
mg/L, add an additional 25 mg
ascorbic acid per each 5 mg/L
chlorine

•	Cool to < 6°C; keep from
freezing









Non-Drinking
Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 7 days if
not acidified

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Adjust to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).



5030C / 8260D

(EPA SW-846)
[Tier I]



Solid

5-g coring device or
amber VOA vial

AND

1 - 2 oz. glass jar
(if needed to
determine percent
moisture)

Coring device (If coring device
is to be cleaned and reused,
samples can be transferred to
an amber VOA vial.)

AND glass jar (if needed to
determine percent moisture)

14 days iffrozen; 48
hours if not frozen

Cool to < 6°C; freeze within 48
hours to < negative (-) 7°C
[Coring tools should not be
frozen below -20°C.]

Wipe outside of coring device or container
clean using a damp, then dry cloth. Place
the coring device or container in a zipper
bag. Pack samples as described in
Footnote (4).



5035A / 8260D

(EPA SW-846)
[Tier I]



Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

SAM Companion - Sample Collection Information Document

A1-17

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Dichlorvos

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier I]

Drinking Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Method 525.2:

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

525.2/525.3 (EPA OW)
[Tier I]

•	Add sodium sulfite to empty
sample container to remove
residual chlorine from treated
water samples'51

•	Acidify to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Method 525.3 (to emDtv

sample container):

•	Add 4 mg ascorbic acid to
remove residual chlorine from
treated water samples

•	Add 14 mg trisodium EDTA,
and 400 mg potassium
dihydrogen citrate

•	Cool to < 6°C; keep from
freezing

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

3535A / 8270E

(EPA SW-846)
[Tier I]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier II]

Dicrotophos

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier I]

Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

3535A / 8270E

(EPA SW-846)
[Tier I]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier II]

SAM Companion - Sample Collection Information Document

A1-18

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Diesel Range Organics

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Diesel fuel, 3,
flammable, UN1203

3520C / 3535A/ 8015D

(EPA SW-846)

[Tier I]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C





3541 / 3545A / 8015D

(EPA SW-846)

[Tier I]



Wipes

1 wipe/100 cm2or
1 wipe/ft2











3570 / 8290A Appendix A /
8015D (EPA SW-846)

[Tier I]

Diisopropyl methyl-phosphonate (DIMP)
(degradation product of GB)

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,

UN3464

TO-10A (EPA ORD)
[Tier III]



Drinking Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Prior to sample collection,
add 400 pL of ammonium
acetate concentrated stock
and 80 |jL of concentrated
sodium omadine stock to
sample container. [If volumes
other than 40-mL are
collected, the concentration of
ammonium acetate and
sodium omadine in the sample
should be 1.5 g/L and 64
mg/L, respectively.]

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, liquid,
n.o.s, 6.1, Poison,
UN3278

538 (EPA OW)
[Tier I]



Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

24 hours

Cool to < 6°C; keep from
freezing

• Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51 ( '





D7597-16 (ASTM)
[Tier II]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

Cool to < 6°C



Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,

E2866-12 (ASTM)
[Tier II]



Wipes

1 wipe/100 cm2or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days





n.o.s, 6.1, Poison,
UN3464

EPA/600/R-13/224 (EPA)
[Tier II]

SAM Companion - Sample Collection Information Document

A1-19

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Dimethylphosphoramidic acid (degradation product of GA)

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,
n.o.s, 6.1, Poison,
UN3464

TO-10A (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

24 hours

Cool to < 6°C; keep from
freezing

• Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51 (8>

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, liquid,
n.o.s, 6.1, Poison,
UN3278

D7597-16 (ASTM)
[Tier III]

Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,
n.o.s, 6.1, Poison,
UN3464

E2866-12 (ASTM)
[Tier III]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

•	Cool to < 6°C

•	Protect from light

EPA/600/R-13/224 (EPA)
[Tier III]

Dimethylphosphite

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

Analyze immediately/ as
soon as possible

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,
n.o.s, 6.1, Poison,
UN3464

TO-10A (EPA ORD)
[Tier II]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Analyze immediately/ as
soon as possible

Cool to < 6°C

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of the vial is dry, then seal the
vial with non-reactive tape or film (e.g.,
fluoropolymer-based), and wrap with
bubble wrap. Pack samples as described
in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm or
1 wipe/ft2

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

UN3464

EPA/600/R-16/114 (EPA)
[Tier II]

Diphacinone

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Prior to collection of treated
water samples, add 10 mg of
dry ascorbic acid to sample
container for removal of
residual chlorine

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Coumarin derivative
pesticides, liquid, toxic,
6.1, Poison, UN3023

D7644-16 (ASTM)
[Tier II]

Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Coumarin derivative
pesticides, solid, toxic,
6.1, Poison, UN3026

3541 /3545A (EPA SW-846) /
D7644-16 (ASTM)

[Tier III]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

3570 / 8290A Appendix A

(EPA SW-846)/

D7644-16 (ASTM)

[Tier III]

SAM Companion - Sample Collection Information Document

A1-20

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Disulfoton

Air

12 - 240 L

(flow rate = 0.2 to 1

L/min)

Filter/solid sorbent tube (OVS-
2 tube: 13 mm quartz filter,
XAD-2, [270 mg/140 mg])

30 days if frozen; 10
days if not frozen

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

5600 (NIOSH)
[Tier I]

Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Analyze immediately/ as
soon as possible

Method 525.2:

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

525.2/525.3 (EPA OW)
[Tier II]

•	Add sodium sulfite to empty
sample container to remove
residual chlorine from treated
water samples'51

•	Acidify to pH < 2 with 1;1 HCI

•	Cool to < 6°C; keep from
freezing

Method 525.3 (to emDtv

sample container):

•	Add 4 mg ascorbic acid to
remove residual chlorine from
treated water samples

•	Add 14 mg trisodium EDTA,
and 400 mg potassium
dihydrogen citrate

•	Cool to < 6°C; keep from
freezing

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier II]

Disulfoton sulfone oxon

Air

12 - 240 L

(flow rate = 0.2 to 1

L/min)

Filter/solid sorbent tube (OVS-
2 tube: 13 mm quartz filter,
XAD-2, [270 mg/140 mg])

30 days if frozen; 10
days if not frozen

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

5600 (NIOSH)
[Tier III]

Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Analyze immediately/ as
soon as possible

Method 525.2:

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

525.2/525.3 (EPA OW)
[Tier III]

•	Add sodium sulfite to empty
sample container to remove
residual chlorine from treated
water samples'51

•	Acidify to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Method 5?5 3 (to errmtv

sample container):

•	Add 4 mg ascorbic acid to
remove residual chlorine from
treated water samples

•	Add 14 mg trisodium EDTA,
and 400 mg potassium
dihydrogen citrate

•	Cool to < 6°C; keep from
freezing

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier III]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier III]

SAM Companion - Sample Collection Information Document

A1-21

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Disulfoton sulfoxide
Disulfoton sulfoxide oxon

Air

12 - 240 L

(flow rate = 0.2 to 1

L/min)

Filter/solid sorbent tube (OVS-
2 tube: 13 mm quartz filter,
XAD-2, [270 mg/140 mg])

30 days if frozen;
10 days if not frozen

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

5600 (NIOSH)
[Tier III]

Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Analyze immediately/ as
soon as possible

Method 525.2:

•	Add sodium sulfite to empty
sample container to remove
residual chlorine from treated
water samples''

•	Acidify to pH <2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Method 5?5 3 Cto emntv
sample container):

•	Add 4 mg ascorbic acid to
remove residual chlorine from
treated water samples

•	Add 14 mg trisodium EDTA,
and 400 mg potassium
dihydrogen citrate

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

525.2/525.3 (EPA OW)
[Tier II]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier III]

Wipes

1 wipe/100 cm2or
1 wipe/ft

EPA/600/R-16/114 (EPA)
[Tier III]

1,4-Dithiane

(degradation product of HD)

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type.

NA

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Cool to < 6°C; keep from
freezing

For treated water samples-
Preservatives/dechlorinating
agents not required, but the
analyte is not affected by the
following, if added to address
other analytes:

•	Sodium sulfite (5)

•	1.6- 2.0 mg NH4CI

For all other samnles-

•	Adjust pH < 2 with 1:1 HCI

Wipe outside of container clean using a
damp, then dry cloth. Seal the vial with non
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label

EPA/600/R-16/114

(EPA)

[Tier II]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier II]

SAM Companion - Sample Collection Information Document

A1-22

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

EA2192 [S-2-(diisopropylamino)ethyl methylphosphonothioic
acid]

(hydrolysis product of VX)

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach
wipe, taking care not to
obscure labeling.

TO-10A (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist



EPA/600/R-15/097 (EPA)
[Tier II]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C

punctures, then into metal can before
placing in cooler.



3541/3545A

(EPA SW-846) /
EPA/600/R-15/097 (EPA)
[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days







3570 / 8290A Appendix A

(EPA SW-846) /
EPA/600/R-15/097 (EPA)
[Tier III]

Ethyl methylphosphonic acid (EMPA)
(degradation product of VX)

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,
n.o.s, 6.1, Poison,
UN3464

TO-10A (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

24 hours

Cool to < 6°C; keep from
freezing

• Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51 (8)

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, liquid,
n.o.s, 6.1, Poison,
UN3278

D7597-16 (ASTM)

[Tier III - Drinking Water]
[Tier II - Non-Drinking Water]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

Cool to < 6°C



Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,
n.o.s, 6.1, Poison,

E2866-12 (ASTM)
[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days





UN3464

EPA/600/R-13/224 (EPA)
[Tier II]

SAM Companion - Sample Collection Information Document

A1-23

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Ethyldichloroarsine (ED)

Air

1-L or 6-L canister
(depending on
suspected
concentration)* 1

Specially prepared stainless
steel canister

For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the
sample is collected using a
pump and flow control
arrangement to achieve a
typical 101 - 202 kPa (15 - 30

psig)

Analyze immediately/ as
soon as possible

None

Ship canister in container provided by
laboratory

Standard carrier
shipping label AND
Ethyldichloroarsine, 6.1,
Poison, UN1892

TQ-15 (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Until holding time data
are available, analyze
immediately/ as soon as
possible

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



3535A / 8270E

(EPA SW-846)
[Tier III]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C





3541 / 3545A / 8270E

(EPA SW-846)

[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days







9102 (NIOSH)/
8270E (EPA SW-846)
[Tier III]

Ethylene oxide

Air

1-L or 6-L canister
(depending on
suspected
concentration)'61

Specially prepared stainless
steel canister

For subatmospheric pressure
sampling, the canister is
evacuated to 0.05 mm Hg; for
pressurized sampling, the
sample is collected using a
pump and flow control
arrangement to achieve a
typical 101 - 202 kPa (15 - 30
psig)

30 days

None

Ship canister in container provided by
laboratory

Standard carrier
shipping label AND
Ethylene oxide
(concentration
dependent, refer to 40
CFR 172.101 for more
information)

TO-15 (EPA ORD)
[Tier I]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 7 days if
not acidified

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Adjust to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).



5030C / 8260D

(EPA SW-846)
[Tier II]



Solid

5-g coring device or
amber VOA vial

AND

1 - 2 oz. glass jar
(if needed to
determine percent
moisture)

Coring device (If coring device
is to be cleaned and reused,
samples can be transferred to
an amber VOA vial.)

AND glass jar (if needed to
determine percent moisture)

14 days if frozen; 48
hours if not frozen

Cool to < 6°C; freeze within 48
hours to < negative (-) 7°C
[Coring tools should not be
frozen below -20°C.]

Wipe outside of coring device or container
clean using a damp, then dry cloth. Place
the coring device or container in a zipper
bag. Pack samples as described in
Footnote (4).



5035A / 8260D

(EPA SW-846)
[Tier II]



Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

SAM Companion - Sample Collection Information Document

A1-24

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Fenamiphos

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier II]



Drinking Water

1 L

Amber glass or fluoropolymer

Analyze immediately/ as

Method 525.2:

Wipe outside of container clean using a

Standard carrier

525.2/525.3 (EPA OW)





container with fluoropolymer-
lined septum or lid

soon as possible

•	Add sodium sulfite to empty
sample container to remove
residual chlorine from treated
water samples'51

•Acidify to pH <2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Method 5?5 3 Ctn errmtv

bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of the vial is dry, then seal the
vial with non-reactive tape or film (e.g.,
fluoropolymer-based), and wrap with
bubble wrap. Pack samples as described
in Footnote (4).

shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

[Tier I]











sample container):

•	Add 4 mg ascorbic acid to
remove residual chlorine from
treated water samples

•	Add 14 mg trisodium EDTA,
and 400 mg potassium
dihydrogen citrate

•	Cool to < 6°C; keep from
freezing









Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Analyze immediately/ as
soon as possible.

Cool to < 6°C; keep from
freezing





EPA/600/R-16/114 (EPA)
[Tier II]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of the vial is dry, then seal the
vial with non-reactive tape or film (e.g.,
fluoropolymer-based), and wrap with
bubble wrap. Pack samples as described
in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]



Wipes

1 wipe/100 cm2or
1 wipe/ft







EPA/600/R-16/114 (EPA)
[Tier II]

Fentanyl

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organic, n.o.s., liquid,
6.1, Poison, UN2810

3520C / 3535A
(EPA SW-846) /
J. Chromatogr. A (2011) 1218:
1620-1649

[Tier III]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C



Standard carrier
shipping label AND
Organic, n.o.s., solid,
6.1, Poison, UN2811

3541/3545A
(EPA SW-846)/
J. Chromatogr. A (2011) 1218:
1620-1649

[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Glass container with
fluoropolymer-lined lid

7 days

•	Cool to < 6°C

•	Protect from light

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based). Wrap glass containers with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Organic, n.o.s., solid,
6.1, Poison, UN2811

SOP L-A-309 Rev.O /
SOP L-A-310 Rev.1

(EPA PH I LIS)

[Tier II]

SAM Companion - Sample Collection Information Document

A1-25

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Fluoride

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Drinking Water

Non-Drinking
Water

40 mL

Fluoropolymer, plastic or glass
container

28 days

Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label

300.1, Rev 1.0 (EPA OW)
[Tier I]

Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Fluoroacetamide

Air

TBD

TBD

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Fluoroacetamide, 6.1,
Poison, UN 1687

J. Chromatogr. B (2008)
876(1): 103-108

[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Screw-cap VOA vial with
fluoropolymer-lined septum

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).

J. Chromatogr. B (2008)
876(1): 103-108

[Tier II]

Solid

40 mL

Screw-cap VOA vial with
fluoropolymer-lined septum

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C

J. Chromatogr. B (2008)
876(1): 103-108

[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

J. Chromatogr. B (2008)
876(1): 103-108

[Tier III]

Fluoroacetic acid and fluoroacetate salts (analyze as
fluoroacetate ion)

Air

Up to 480 L (at 1.5-
2.0 L/min)

Cellulose ester membrane
filter / cleaned and rinsed
glass or plastic bottles

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Fluoroacetic acid, 6.1,
Poison, UN2642

S301-1 (NIOSH)

J. Chromatogr. A (2007) 1139:

271-278

[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Screw-cap VOA vial with
fluoropolymer-lined septum

28 days

•	Add ascorbic acid (250 mg/L,
final concentration) to remove
residual chlorine and sodium
omadine (64 mg/L, final
concentration) as an
antimicrobial

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a bleach
wipe, taking care not to affect any labeling.
Make sure bleach is removed and the
outside of the vial is dry, then seal the vial
with non-reactive tape or film (e.g.,
fluoropolymer-based), and wrap with
bubble wrap. Pack samples as described
in Footnote (4).

EPA/600/R-18/056 (EPA)
[Tier II]

Solid

250 mL

Wide-mouth glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

Cool to < 6°C

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

J. Chromatogr. A (2007) 1139:
271-278

[Tier III]

Wipes

1 wipe/100 cm or
1 wipe/ft2

J. Chromatogr. A (2007) 1139:
271-278

[Tier III]

SAM Companion - Sample Collection Information Document

A1-26

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

2-Fluoroethanol

Air

2 (at 5 ppm) - 35 L
(flow rate = 0.01 -
0.2 L/min)

Solid sorbenttube (petroleum
charcoal [100 mg/50 mg])

14 days

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap tubes with
plastic (not rubber) caps. Place tubes in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label

2513 (NIOSH)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 7 days if
not acidified

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Adjust to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film, and wrap with bubble
wrap. Pack samples as described in
Footnote (4).



5030C / 8260D

(EPA SW-846)
[Tier III]



Solid

5-g coring device or
amber VOA vial

AND

1 - 2 oz. glass jar
(if needed to
determine percent
moisture)

Coring device (If coring device
is to be cleaned and reused,
samples can be transferred to
an amber VOA vial.)

AND glass jar (if needed to
determine percent moisture)

14 days if frozen;
48 hours if not frozen

Cool to < 6°C; freeze within 48
hours to < negative (-) 7°C
[Coring tools should not be
frozen below -20°C.]

Wipe outside of coring device or container
clean using a damp, then dry cloth. Place
the coring device or container in a zipper
bag. Pack samples as described in
Footnote (4).



5035A / 8260D

(EPA SW-846)
[Tier III]



Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Fluorosilicic acid (analyze as fluoride)

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

40 mL

Screw-cap VOA vial with
fluoropolymer-lined septum

28 days

Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label

300.1, Rev 1.0

(EPA OW)
[Tier I]



Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Formaldehyde

Air

1 L (at 0.25 mg/m3)
- 15 L (at 2.5
mg/m3)

(flow rate = 0.03 -
1.5 L/min)

Dinitrophenylhydrazine
(DNPH)-coated silica gel
cartridge

30 days

Cool to < 6°C

Place end caps onto the cartridge and seal
sampler in an envelope. Place cartridge in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Formaldehyde
(concentration
dependent, refer to 49
CFR 172.101 for more
information)

2016 (NIOSH)
[Tier I]



Drinking Water

30 mL

Amber glass or fluoropolymer
container with polypropylene
screw cap equipped with
fluoropolymer-lined septum

7 days

•	Prior to collecting treated
water samples, add 15 mg
copper sulfate pentahydrate
and 15 mg NH4CI to sample
container

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



556.1 (EPA OW)
[Tier I]



Non-Drinking
Water

100 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

3 days

•	Preserve to pH 5.0 with 1:1
HCI orNaOH

•	Cool to < 6°C; keep from
freezing

•	Add 10 mg NH4CI to remove
residual chlorine from treated
water samples





8315A (EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or

3 days

Cool to < 6°C





8315A (EPA SW-846)
[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

lid









3570 / 8290A Appendix A /
8315A (EPA SW-846)

[Tier III]

SAM Companion - Sample Collection Information Document

A1-27

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Gasoline Range Organics

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 7 days if
not acidified

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Adjust to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Gasoline identification
set, 2.3, Poison gas,
NA9035

5030C / 8015D

(EPA SW-846)
[Tier I]



Solid

5-g coring device or
amber VOA vial

AND

1 - 2 oz. glass jar
(if needed to
determine percent
moisture)

Coring device (If coring device
is to be cleaned and reused,
samples can be transferred to
an amber VOA vial.)

AND glass jar (if needed to
determine percent moisture)

14 days if frozen;
48 hours if not frozen

Cool to < 6°C; freeze within 48
hours to < negative (-) 7°C
[Coring tools should not be
frozen below -20°C.]

Wipe outside of coring device or container
clean using a damp, then dry cloth. Place
the coring device or container in a zipper
bag. Pack samples as described in
Footnote (4).



5035A / 8015C

(EPA SW-846)
[Tier I]



Wipes

1 wipe/100 cm or
1 wipe/ft2

Wide-mouth glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

•	Cool to < 6°C

•	Protect from light

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



3570 / 8290A Appendix A /
8015D (EPA SW-846)

[Tier I]

Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
RDX, 1.1, Explosive,
UN0483(9)

3535A / 8330B

(EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C





8330B (EPA SW-846)
[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days







3570 / 8290A Appendix A /
8330B (EPA SW-846)

[Tier I]

Hexamethylene
triperoxidediamine (HMTD)

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape orfilm (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
HMTD, 1.1, Explosive'91

3535A / 8330B
(EPA SW-846)/

Analyst (2001) 126: 1689-1693

[Tier II]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C





8330B (EPA SW-846) /

Analyst (2001) 126: 1689-1693

[Tier II]



Wipes

1 wipe/100 cm2or
1 wipe/ft

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days







3570 / 8290A Appendix A

(EPA SW-846) /

Analyst (2001) 126: 1689-1693

[Tier III]

SAM Companion - Sample Collection Information Document

A1-28

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.1lytic.1l
Methods - SAM 2022"

Hydrogen bromide

Air

30 - 600 L (flow rate
= 2 L/min)

Two filters in series separated
with chemically inert spacer:

•	pre-filter: 37-mm diameter
quartz fiber

•	sampling filter: 37-mm
diameter quartz fiber
impregnated with 1 M 500-|jL
N 82003

28 days

Cool to < 6°C

Wipe outside of filter clean using a damp,
then dry cloth. Place filter in double plastic
bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Hydrogen bromide, 2.3,
Poisonous gas,
UN1048. Corrosive,
UN1052

7907 (NIOSH)
[Tier 1]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Hydrogen chloride

Air

30 - 600 L (flow rate
= 2 L/min)

Two filters in series separated
with chemically inert spacer:

•	pre-filter: 37-mm diameter
quartz fiber

•	sampling filter: 37-mm
diameter quartz fiber
impregnated with 1 M 500-jjL
Na2C03

28 days

Cool to < 6°C

Wipe outside of filter clean using a damp,
then dry cloth. Place filter in double plastic
bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Hydrogen chloride, 2.3,
Poisonous gas,
UN1050. Corrosive,
UN1052

7907 (NIOSH)
[Tier 1]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Hydrogen cyanide

Air

2 (at 5 ppm) - 90 L
(flow rate = 0.05 -
0.2 L/min)

Solid sorbent tube (soda lime
[600 mg/200 mg])

14 days

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap tubes with
plastic (not rubber) caps. Place tubes in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Hydrogen cyanide, 2.3,
Poisonous gas,
UN1051

6010 (NIOSH)
[Tier I]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Hydrogen fluoride

Air

30 - 600 L (flow rate
= 2 L/min)

Two filters in series separated
by a chemically inert spacer:

•	pre-filter: cellulose nitrate,
0.8 |jm pore size, 37-mm
diameter

•	treated filter: cellulose nitrate
impregnated with 0.75 M
Na2C03, 0.8 |jm pore size, 37-
mm diameter

28 days

Cool to < 6°C

Wipe outside of filter clean using a damp,
then dry cloth. Place filter in double plastic
bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Hydrogen fluoride, 2.3,
Poisonous gas,
Corrosive, UN1052

7906 (NIOSH)
[Tier I]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

SAM Companion - Sample Collection Information Document

A1-29

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Hydrogen sulfide

Air

1.2 (at 10ppm)-40
L (flow rate = 0.1 -
1.5 L/min; 0.2 L/min
is recommended)

Filter/solid sorbent tube
(0.5 |jm/coconut shell charcoal
[400 mg/200 mg])

21 days

Cool to < 6°C

Wipe filter and sorbent tube clean, using a
damp, then dry cloth. Place filter and
sorbent tube in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Hydrogen sulfide, 2.3,
Poisonous gas, 2.1,
Flammable gas,
Corrosive, UN1053

6013 (NIOSH)
[Tier I]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Isopropyl methylphosphonic acid (IMPA) (degradation
product of GB)

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,
n.o.s, 6.1, Poison,
UN3464

TO-10A (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

24 hours

Cool to <6°C; keep from
freezing

• Add sodium thiosulfateto
remove residual chlorine from
treated water samples'51 (8)

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, liquid,
n.o.s, 6.1, Poison,
UN3278

D7597-16 (ASTM)

[Tier III - Drinking Water]
[Tier II - Non-Drinking Water]

Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,
n.o.s, 6.1, Poison,
UN3464

E2866-12 (ASTM)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

EPA/600/R-13/224 (EPA)
[Tier II]

Kerosene

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfateto
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Kerosene, 3,
Flammable, UN1223

3520C / 3535A / 8015D

(EPA SW-846)

[Tier I]

Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C

3541 / 3545A / 8015D

(EPA SW-846)

[Tier I]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

•	Cool to < 6°C

•	Protect from light

3570 / 8290A Appendix A /
8015D

(EPA SW-846)

[Tier I]

SAM Companion - Sample Collection Information Document

A1-30

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Lead arsenate
(analyze as total arsenic)

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Lead arsenate, 6.1,
Poison, UN1617

IO-3.11
10-3.4/10-3.5

(EPA ORD)
[Tier 1]



Drinking Water

125 mL

Fluoropolymer, plastic or glass
container

6 months with
acidification. Samples
must be acidified within
14 days of collection.

• Acidify to pH < 2 with
HN03(7> [If arsenic species are
to be determined, remove
unpreserved aliquot prior to
sample preservation.]

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack



200.7/200.8 (EPA OW)
[Tier 1]



Non-Drinking
Water

125 mL





• Acidified samples can be
cooled to < 6°C; keep from
freezing

samples as described in Footnote (4).



3015A /

6010D /6020B (EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

Analyze immediately/ as
soon as possible

Cool to < 6°C





3050B / 3051A /

6010D /6020B (EPA SW-846)

[Tier I]



Wipes

1 wipe/100 cm or
1 wipe/ft











9102 (NIOSH)

6010D /6020B (EPA SW-846)
[Tier I]

Lewisite 1 (L-1)
Lewisite 2 (L-2)
Lewisite 3 (L-3)

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach
wipe, taking care not to
obscure labeling.

IO-3.1

IO-3.4/IO-3.5

(EPA ORD)
[Tier I]



Drinking Water

Non-Drinking
Water

40 mL

VOA vial with fluoropolymer-
lined cap

14 days

Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a bleach
wipe, taking care not to affect any labeling.
Make sure bleach is removed and the
outside of vial is dry, then wrap it with
bubble wrap. Place in gallon plastic

EPA/600/R-15/258 (EPA)

[Tier II - Lewisite 1]

[Tier III - Lewisites 2 and 3]



Solid

40 mL

VOA vial with fluoropolymer-
lined cap

Analyze immediately/ as
soon as possible

Cool to < 6°C

(polypropylene or polyethylene) bag with
zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.



EPA/600/R-15/258 (EPA)

[Tier II - Lewisite 1]

[Tier III - Lewisites 2 and 3]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

VOA vial with fluoropolymer-
lined cap

14 days







EPA/600/R-15/258 (EPA)

[Tier II - Lewisite 1]

[Tier III - Lewisites 2 and 3]

Lewisite oxide

(degradation product of Lewisite)
(analyze as total arsenic)

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of

IO-3.1 /
10-3.4/10-3.5

(EPA ORD)
[Tier 1]



Drinking Water

Non-Drinking
Water

40 mL

VOA vial with fluoropolymer-
lined septum

14 days

•	Acidify to pH < 2 with HN03

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a bleach
wipe, taking care not to affect any labeling.
Make sure bleach is removed and the
outside of vial is dry, then wrap it with
bubble wrap. Place in gallon plastic
(polypropylene or polyethylene) bag with
zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

package with a bleach
wipe, taking care not to
obscure labeling.

EPA/600/R-15/258 (EPA)
[Tier III]



Solid

40 mL

VOA vial with fluoropolymer-
lined septum

14 days

Cool to < 6°C



EPA/600/R-15/258 (EPA)
[Tier III]



Wipes

1 wipe/100 cm or
1 wipe/ft









EPA/600/R-15/258 (EPA)
[Tier III]

Mercuric chloride (analyze as total mercury)

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

125 mL

Fluoropolymer, plastic or glass
container

28 days

Acidify to pH < 2 with HN03(7)

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Mercuric chloride, 6.1,
Poison, UN 1624

245.1 (EPA OW)
[Tier I]



Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

28 days

Cool to < 6°C



7473 / 7471B (EPA SW-846)
[Tier I]



Wipes

1 wipe/100 cm2or
1 wipe/ft2











9102 (NIOSH)/

7473 / 7471B (EPA SW-846)

[Tier I]

SAM Companion - Sample Collection Information Document

A1-31

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Mercury, Total

Air

Typical flow rate is
30 L/min for 12 - 24
hours

Trap apparatus:

•	Vapor phase: Sampling
pumps specially designed for
trace-level pollutant sampling;
vapor phase Hg collected
using two gold traps in series,
attached to a fluoropolymer
filter pack

•	Particle phase: Glass fiber
filter

7 days

[Particle-phase Hg filters
can be stored indefinitely
at negative
(-) 40°C.]

Cool to < 6°C

Wipe the outside of apparatus clean, cap
both ends. Place in double plastic bags
and wrap with bubble wrap. Pack samples
as described in Footnote (4).

Standard carrier
shipping label AND
Mercury, 8, Corrosive,
UN2809

IO-5 (EPA ORD)
[Tier I]



Drinking Water

Non-Drinking
Water

125 mL

Fluoropolymer, plastic or glass
container

28 days

Acidify to pH < 2 with HN03(7>
[If dissolved mercury is to be
determined, filter the sample
prior to acidification.]

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



245.1 (EPAOW)
[Tier I]



Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

28 days

Cool to < 6°C





7473 / 7471B (EPA SW-846)
[Tier I]



Wipes

1 wipe/100 cm or
1 wipe/ft2











9102 (NIOSH)/

7473 / 7471B (EPA SW-846)

[Tier I]

Methamidophos

Air

TBD

TBD

Until holding time data
are available, analyze
immediately/ as soon as
possible

• Cool to < 6°C

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

J. Chromatogr. A (2007)
1154(1): 3-25

[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Prior to collection, add 400
|jL of ammonium acetate
concentrated stock and 80 jj L
of concentrated sodium
omadine stock to sample
container. [If volumes other
than 40-mL are collected, the
concentration of ammonium
acetate and sodium omadine
in the sample should be 1.5
g/L and 64 mg/L, respectively.]

•	Cool to < 6°C; keep from

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

538 (EPA OW)
[Tier I]



Solid

100 mL

Wide-mouth glass or
fluoropolymer container with
fluoropolymer-lined lid

Until holding time data
are available, analyze
immediately/ as soon as
possible

•	Cool to < 6°C

•	Protect from light



Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

J. Env. Sci. Health (2014)49:
23-34 /

538 (EPA OW)

[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2









J. Chromatogr. A (2007)
1154(1): 3-25

[Tier III]

SAM Companion - Sample Collection Information Document

A1-32

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Methomyl

Air

12 - 480 L

(flow rate = 0.1-1

L/min)

Filter/solid sorbenttube:

•	13- mm quartz fiber filter

•	OVS-2 tube (XAD-2 resin
[270mg/140 mg])

30 days if frozen; 7 days
at room temperature
(24°C)

Cool to < 6°C

Wpe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757

5601 (NIOSH)
[Tier I]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

28 days

•	Prior to collection, add 0.4 g
potassium dihydrogen citrate
and 3.2 - 4.8 mg sodium
thiosulfate to sample container

•	Agitate for 1 minute after
collection

•	Cool to < 6°C; keep from
freezing

Wpe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Carbamate pesticides,
liquid, toxic, 6.1,
Poison, UN2992

531.2 (EPA OW)
[Tier I]

Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

Cool to < 6°C

Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757

8318A (EPA SW-846)
[Tier II]

Wipes

1 wipe/100 cm or
1 wipe/ft2

3570 / 8290A Appendix A /
8318A (EPA SW-846)

[Tier III]

Methoxyethylmercuric acetate (analyze as total mercury)

Air

Typical flow rate of
30 L/min for 12-24
hours

Trap apparatus:

•	Vapor phase: Sampling
pumps specially designed for
trace-level pollutant sampling;
vapor phase Hg collected
using two gold traps in series,
attached to a fluoropolymer
filter pack

•	Particle phase: Glass fiber
filter

7 days

[Particle-phase Hg filters
can be stored indefinitely
at negative
(-) 40°C.]

Cool to < 6°C

Wpe the outside of trap apparatus clean,
cap both ends. Place in double plastic
bags and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Mercury compounds,
solid, n.o.s, 6.1, Poison,
UN2025

IO-5 (EPA ORD)
[Tier I]

Drinking Water

Non-Drinking
Water

125 mL

Fluoropolymer, plastic or glass
container

28 days

Acidify to pH < 2 with HN03(7>

Wpe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Mercury compounds,
liquid, n.o.s, 6.1,
Poison, UN2024

245.1 (EPA OW)
[Tier I]

Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

28 days

Cool to < 6°C

Standard carrier
shipping label AND
Mercury compounds,
solid, n.o.s, 6.1, Poison,
UN2025

7473 / 7471B (EPA SW-846)
[Tier I]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

9102 (NIOSH)/

7473 / 7471B (EPA SW-846)

[Tier I]

SAM Companion - Sample Collection Information Document

A1-33

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Methyl acrylonitrile

Air

120 L (flow rate = 1
L/min)

Filter/solid sorbenttube(13-
mm quartz fiber filter)

•	OVS-7 tube (XAD-7 resin)

•	OVS-2 (XAD-2 resin)

•	OVS-SG (silica gel)

13 days

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Methyl acrylonitrile, 3.0,
Flammable Liquid, 6.1,
Poison, UN3079

PV2004 (OSHA)
[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 24 hours if
not acidified

Prior to collection of treated
water samples, add 25 mg
ascorbic acid to container for
removal of residual chlorine

Method 524.2:

Wipe outside of vial clean using a damp,
then dry cloth. Seal the-containerwith non-
reactive tape or film (e.g., fluoropolymer-
based). Wrap glass containers with bubble
wrap. Pack samples as described in
Footnote (4).

524.2/524.3/524.4

(EPA OW)

[Tier II]

• Adjust to pH < 2with 1:1 HCI
Methods 524.3 and 524.4:

• Adjust to pH < 2 with maleic
acid

For all samoles:

•	If residual chlorine is > 5
mg/L, add an additional 25 mg
ascorbic acid per each 5 mg/L
chlorine

•	Cool to < 6°C; keep from
freezing

Solid

5-g coring device or
amber VOA vial

AND

1 - 2 oz. glass jar
(if needed to
determine percent
moisture)

Coring device (If coring device
is to be cleaned and reused,
samples can be transferred to
an amber VOA vial.)

AND glass jar (if needed to
determine percent moisture)

14 days if frozen;
48 hours if not frozen

Cool to < 6°C; freeze within 48
hours to < negative (-) 7°C.
[Coring tools should not be
frozen below -20°C.]

Wipe outside of coring device or container
clean using a damp, then dry cloth. Place
the coring device or container in a zipper
bag. Pack samples as described in
Footnote (4).

5035A / 8260D

(EPA SW-846)
[Tier II]

Wipes

1 wipe/100 cm2or
1 wipe/ft2

Screw-cap VOA vial with
fluoropolymer-lined septum or
lid

14 days

•	Cool to < 6°C

•	Protect from light

Wipe outside of vial clean using a damp,
then dry cloth. Seal the container with non-
reactive tape or film (e.g., fluoropolymer-
based). Wrap glass containers with bubble
wrap. Pack samples as described in
Footnote (4).

3570 / 8290A Appendix A /
8260D (EPA SW-846)

[Tier III]

3-Methyl fentanyl

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organic, n.o.s., liquid,
6.1, Poison, UN2810

3520C / 3535A
(EPA SW-846)/
J. Chromatogr. B (2014) 962:
52-58

[Tier III]

Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organic, n.o.s., solid,
6.1, Poison, UN2811

3541/3545A
(EPA SW-846)/
J. Chromatogr. B (2014) 962:
52-58

[Tier III]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Glass container with
fluoropolymer-lined lid

7 days

•	Cool to < 6°C

•	Protect from light

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based). Wrap glass containers with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Organic, n.o.s., solid,
6.1, Poison, UN2811

SOP L-A-309 Rev.O /
SOP L-A-310 Rev.1

(EPA PH I LIS)

[Tier II]

SAM Companion - Sample Collection Information Document

A1-34

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Methyl fluoroacetate (analyze as fluoroacetate ion)

Air

Up to 480 L (flow
rate = 1.5-2.0
L/min)

Cellulose ester membrane
filter/cleaned and rinsed glass
or plastic bottles

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Fluoroacetic acid, 6.1,
Poison, UN2642

S301-1 (NIOSH)

J. Chromatogr. A (2007) 1139:

271-278

[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Screw-cap VOA vial with
fluoropolymer-lined septum

28 days

•	Add ascorbic acid (250 mg/L,
final concentration) to remove
residual chlorine and sodium
omadine (64 mg/L, final
concentration) as an
antimicrobial

•	Cool to <6°C; keep from
freezing

Wipe outside of vial clean using a bleach
wipe, taking care not to affect any labeling.
Make sure bleach is removed and the
outside of the vial is dry, then seal the vial
with non-reactive tape orfilm (e.g.,
fluoropolymer-based), and wrap with
bubble wrap. Pack samples as described
in Footnote (4).



EPA/600/R-18/056 (EPA)
[Tier II]



Solid

250 mL

Wide-mouth glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

Cool to < 6°C

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



J. Chromatogr. A (2007) 1139:
271-278

[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft









J. Chromatogr. A (2007) 1139:
271-278

[Tier III]

Methyl hydrazine

Air

3 (at 0.2 ppm) - 20
L (flow rate = 0.5 to
1.5 L/min)

Bubbler (0.1 M HCI). Seal and
transfer the bubbler to
container for shipping.

5 days

Cool to < 6°C

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass

Standard carrier
shipping label AND
Methyl hydrazine, 6.1,
Poison, UN 1244

3510 (NIOSH)
[Tier I]



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

containers with bubble wrap. Pack
samples as described in Footnote (4).



J. Chromatogr. (1993) 617:
157-162

[Tier II]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C





3541 / 3545A (EPA SW-846)
J. Chromatogr. (1993) 617:
157-162

[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days







3570 / 8290A Appendix A
(EPA SW-846)/
J. Chromatogr. (1993) 617:
157-162

[Tier III]

Methyl isocyanate

Air

15 L (flow rate =
0.05 L/min)

Solid sorbenttube (XAD-7
tubes coated with 0.03 mg of 1-
(2-pyridyl)piperazine)

18 days

•	Cool to < 6°C

•	Protect from light

Wipe outside of tube clean using a damp,
then dry cloth. Cap ends of tube, wrap tube
with OSHA seal, and place in container.
Seal the container with non-reactive tape
or film. Wrap glass containers with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Methyl isocyanate, 6.1,
Poison, UN2480

OSHA 54 [Tier I]



Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

SAM Companion - Sample Collection Information Document

A1-35

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Methyl paraoxon

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

3535A / 8270E

(EPA SW-846)
[Tier III]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier III]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier III]

Methyl parathion

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier I]

Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
treated water samples'1

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

3535A / 8270E

(EPA SW-846)
[Tier I]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier II]

Methylamine

Air

10 L (flow rate = 0.2
L/min)

Solid sorbent tubes containing
XAD-7 resin coated with 10%
NBD chloride (7-chloro-4-
nitrobenzo-2-oxa-1,3-diazole)
by weight

15 days

Cool to < 6°C

Wipe outside of tube clean using a damp,
then dry cloth. Cap ends of tube, wrap tube
with OSHA seal, and place in container.
Seal the container with non-reactive tape
or film (e.g., fluoropolymer-based). Wrap
glass containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND,
Methylamine,
anhydrous, 2.1,
Flammable gas,
UN1061

OSHA 40

[Tier I]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

SAM Companion - Sample Collection Information Document

A1-36

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Methylphosphonic acid (MPA)
(degradation product of VX, GB, & GD)

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,
n.o.s, 6.1, Poison,
UN3464

TO-10A (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

24 hours

Cool to < 6°C; keep from
freezing

• Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51 (8>

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape orfilm (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, liquid,
n.o.s, 6.1, Poison,
UN3278

D7597-16 (ASTM)

[Tier III - Drinking Water]
[Tier II - Non-Drinking Water]

Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,
n.o.s, 6.1, Poison,
UN3464

E2866-12 (ASTM)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

EPA/600/R-13/224 (EPA)
[Tier II]

Mevinphos

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier II]

Drinking Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Analyze immediately/ as
soon as possible

Method 525.2:

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

525.2/525.3 (EPA OW)
[Tier I]

•	Add sodium sulfite to empty
sample container to remove
residual chlorine from treated
water samples

•	Acidify to pH < 2 with 1:1
HCI(5)

•	Cool to < 6°C; keep from
freezing

Method 5?5 3 Cto errmtv

sample container):

•	Add 4 mg ascorbic acid to
remove residual chlorine from
treated water samples

•	Add 14 mg trisodium EDTA,
and 400 mg potassium
dihydrogen citrate

•	Cool to < 6°C; keep from
freezing

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

3535A / 8270E

(EPA SW-846)
[Tier I]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier II]

SAM Companion - Sample Collection Information Document

A1-37

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Monocrotophos

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

3535A / 8270E

(EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C

samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,

3541 / 3545A/ 8270E

(EPA SW-846)

[Tier I]



Wipes

1 wipe/100 cm or
1 wipe/ft









Poison, UN2783

3570/8290 Appendix A/
8270E (EPA SW-846)
[Tier III]

Mustard, nitrogen (HN-1)

Mustard, nitrogen (HN-2) Mustard, nitrogen (HN-3)

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach

TO-17 (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

wipe, taking care not to
obscure labeling.

EPA/600/R-12/653 (EPA)

[Tier II-HN-1 and HN-3]
[Tier III - HN-2]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C



EPA/600/R-12/653 (EPA)

[Tier II - HN-1 and HN-3]
[Tier III - HN-2]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











EPA/600/R-12/653 (EPA)

[Tier II-HN-1 and HN-3]
[Tier III - HN-2]

Mustard, sulfur (HD)

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach

TO-17 (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Analyze immediately/ as
soon as possible

Cool to < 6°C; keep from
freezing

• Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before

wipe, taking care not to
obscure labeling.

EPA/600/R-16/115 (EPA)
[Tier I]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

placing in cooler.



EPA/600/R-16/115 (EPA)
[Tier I]



Wipes

1 wipe/100 cm or
1 wipe/ft











EPA/600/R-16/115 (EPA)
[Tier I]

SAM Companion - Sample Collection Information Document

A1-38

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

N-Ethyldiethanolamine (EDEA) (degradation product of HN-1)

Air

0.5 - 300 L (flow
rate =0.5-1 L/min)

Impinger (15 mL 2 mM
hexanesulfonic acid).

[Impinger contents are
transferred into VOA vial for
shipping.]

21 days

•	Cool to < 6°C

•	Protect from light

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film, and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Ethanolamine solutions,
8, Corrosive, UN2491

3509 (NIOSH)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



D7599-16 (ASTM)

[Tier II Non-Drinking Water]
[Tier III Drinking Water]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C



3541 /3545A (EPA SW-846) /
EPA/600/R-11/143

(EPA/CDC)

[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

125 mL polypropylene
container with a polypropylene
screw cap

28 days

•	Cool to < 6°C

•	Protect from light





EPA/600/R-11/123

(EPA/CDC)

[Tier II]

Nicotine compounds (analyze as nicotine)

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Nicotine compounds,
liquid, n.o.s, 6.1,
Poison, UN3144

3535A / 8270E

(EPA SW-846)
[Tier II]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C



Standard carrier
shipping label AND

EPA/600/R-16/114 (EPA)
[Tier II]



Wipes

1 wipe/100 cm2or
1 wipe/ft2







solid, n.o.s, 6.1, Poison,
UN1655

EPA/600/R-16/114 (EPA)
[Tier II]

N-Methyl

diethanolamine (MDEA) (degradation product of HN-2)

Air

0.5 - 300 L (flow
rate =0.5-1 L/min)

Impinger (15 mL 2 mM
hexanesulfonic acid)

[Impinger contents are
transferred into VOA vial for
shipping.]

21 days

•	Cool to < 6°C

•	Protect from light

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Ethanolamine solutions,
8, Corrosive, UN2491

3509 (NIOSH)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack



D7599-16 (ASTM)

[Tier III - Drinking Water]
[Tier II - Non-Drinking Water]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C

samples as described in Footnote (4).



3541 /3545A (EPA SW-846) /
EPA/600/R-11/123

(EPA/CDC)

[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

125 mL polypropylene
container with a polypropylene
screw cap

28 days

•	Cool to < 6°C

•	Protect from light





EPA/600/R-11/123

(EPA/CDC)

[Tier II]

SAM Companion - Sample Collection Information Document

A1-39

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.1lytic.1l
Methods - SAM 2022"

1-Methylethyl ester ethylphosphonofluoridic acid (GE)

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach

TQ-17 (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Cool to < 6°C; keep from
freezing

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before

wipe, taking care not to
obscure labeling.

EPA/600/R-16/115 (EPA)
[Tier III]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

placing in cooler.



EPA/600/R-16/115 (EPA)
[Tier III]



Wipes

1 wipe/100 cm or
1 wipe/ft











EPA/600/R-16/115 (EPA)
[Tier III]

Octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX)

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
HMX, 1.1, Explosive,
UN0391(9>

3535A / 8330B

(EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C





8330B (EPA SW-846)
[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days







3570/8290 Appendix A/
8330B (EPA SW-846)
[Tier I]

Osmium tetroxide
(analyze for total osmium)

Air

Up to 1700 m3

Filter (quartz, silica or cellulose
fiber)

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Osmium tetroxide, 6.1,
Poison, UN2471

IO-3.1/IO-3.4 (EPA ORD)
[Tier II]



Drinking Water

Non-Drinking
Water

125 mL

Glass container

6 months with
acidification. Samples
must be acidified within
14 days of collection.

1% NH4OH (7)

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



3015A /
6010D / 6020B

(EPA SW-846)
[Tier II]



Solid

250 mL

Wide-mouth fluoropolymer or
glass container with
fluoropolymer-lined lid

Analyze immediately/ as
soon as possible

Cool to < 6°C



3051A /

6010D /6020B (EPA SW-846)
[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











3051A /

6010D /6020B (EPA SW-846)
[Tier III]

SAM Companion - Sample Collection Information Document

A1-40

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Oxamyl

Air

240 - 480 L
(flow rate = 0.1-1
L/min)

Filter/solid sorbent tube (OVS-
2 tube: 13 mm quartz fiber
filter, XAD-2 [270mg/140 mg])

30 days if frozen; 7 days
at room temperature
(24°C)

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757

5601 (NIOSH)
[Tier I]



Drinking Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

28 days

•	Prior to collection, add 0.4 g
potassium dihydrogen citrate
and 3.2 - 4.8 mg sodium
thiosulfate to sample container

•	Agitate for 1 minute after
collection

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

Standard carrier
shipping label AND
Carbamate pesticides,
liquid, toxic, 6.1,
Poison, UN2992

531.2 (EPA OW)
[Tier I]



Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Prior to collection of treated
water samples, add 10 mg
ascorbic acid to sample
container

•	Acidify to pH < 3.8 with
glacial acetic acid

•	Cool to < 6°C; keep from
freezing





D7645-16 (ASTM)
[Tier II]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or

7 days

Cool to < 6°C



Standard carrier
shipping label AND
Carbamate pesticides,

8318A (EPA SW-846)
[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

lid







solid, toxic, 6.1,
UN2757

3570/8290 Appendix A/
8318A (EPA SW-846)
[Tier III]

Paraoxon

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

3520C / 3535A / 8270E

(EPA SW-846)

[Tier III]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

punctures, then into metal can before
placing in cooler.

Standard carrier
shipping label AND
Organophosphorus

EPA/600/R-16/114 (EPA)
[Tier III]



Wipes

1 wipe/100 cm2or
1 wipe/ft2









pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier III]

Paraquat

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

250 mL

High density PVC or silanized
amber glass bottle fitted with
screw cap

7 days

•	Add 100 mg/L sodium
thiosulfate to remove residual
chlorine from treated water
samples

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist

Standard carrier
shipping label AND
Bipyridilium pesticides,
liquid, 6.1, Toxic,
UN3016

549.2 (EPA OW)
[Tier I]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or

7 days

Cool to < 6°C

punctures, then into metal can before
placing in cooler.

Standard carrier
shipping label AND
Bipyridilium pesticides,

J. Chromatogr. A (2008) 1196-
1197:110-116

[Tier II]



Wipes

1 wipe/100 cm2or
1 wipe/ft2









UN2811

J. Chromatogr. A (2008) 1196-
1197:110-116

[Tier III]

SAM Companion - Sample Collection Information Document

A1-41

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Parathion

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

3520C / 3535A / 8270E

(SW-846)

[Tier I]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

punctures, then into metal can before
placing in cooler.

Standard carrier
shipping label AND
Organophosphorus

EPA/600/R-16/114 (EPA)
[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft







pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]

Pentaerythritol tetranitrate (PETN)

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
PETN, 1.1, Explosive,
UN0411(9>

3535A / 8330B

(EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C





8330B (EPA SW-846)
[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days







3570/8290A Appendix A/
8330B (EPA SW-846)

[Tier I]

Phencyclidine

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label

TO-10A (EPA ORD)
[Tier II]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Cool to < 6°C; keep from
freezing

For treated water samDles:
Preservatives/dechlorinating
agents not required, but the
analyte is not affected by the
following, if added to address
other analytes:

•	Sodium sulfite (5)

•	1.6- 2.0 mg NH4CI

For all other samoles:

•	Can be adjusted to pH < 2
with 1:1 HCI, but this is not
required

Wipe outside of container clean using a
damp, then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).



EPA/600/R-16/114 (EPA)
[Tier II]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C





EPA/600/R-16/114 (EPA)
[Tier II]



Wipes

1 wipe/100 cm2or
1 wipe/ft

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

30 days







9106/9109 (NIOSH)
(Tier II)

SAM Companion - Sample Collection Information Document

A1-42

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Phorate

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier II]

Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

3535A / 8270E

(EPA SW-846)
[Tier I]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier II]

Phorate sulfone
Phorate sulfone oxon

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

250 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

28 days

Add the followina to sarrmle
containers nrior to collection-

•	100 mg/L solid ascorbic acid
(for removal of residual
chlorine from treated water
samples)

•	2g/L 2-chloroacetamide
(antimicrobial preservative)

•	7.75 g/L Trizma® base
(buffering agent)

After collectina the samDle:

•	Cap the container and
agitate until preservative is
dissolved

•	Cool to <10°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

540 (EPA OW)

[Tier I - Phorate sulfone]

[Tier III - Phorate sulfone oxon]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier III]

Wipes

1 wipe/100 cm or
1 wipe/ft

EPA/600/R-16/114 (EPA)
[Tier III]

SAM Companion - Sample Collection Information Document

A1-43

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Phorate sulfoxide
Phorate sulfoxide oxon

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

250 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

28 days

Add the followinn to samnle
containers Drior to collection:

•	100 mg/L solid ascorbic acid
(for removal of residual
chlorine from treated water
samples)

•	2 g/L 2-chloroacetamide
(antimicrobial preservative)

•	7.75 g/L Trizma® base
(buffering agent)

After collectinn the samnle-

•	Cap the container and
agitate until preservative is
dissolved

•	Cool to < 10°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

540 (EPA OW)

[Tier I - Phorate sulfoxide]
[Tier III - Phorate sulfoxide
oxon]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier III]

Wipes

1 wipe/100 cm2or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier III]

Phosgene

Air

240 L (flow rate = 1
L/min)

Silane-treated glass tubes [4-
mm i.d. x 6-mm o.d. x 11-cm
long, and packed with 150
mg/75 mg of pretreated XAD-2
adsorbent coated with 2-HMP
(2-[hydroxymethyl] piperidine)]

19 days

Cool to < 6°C

Wipe outside of tube clean using a damp,
then dry cloth. Cap ends of tube, wrap tube
with OSHA seal, and place in an air tight
container containing activated carbon. Seal
the container with non-reactive tape or film
(e.g., fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Phosgene, 2.3,
Poisonous gas,
UN1076

OSHA 61 [Tier I]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

SAM Companion - Sample Collection Information Document

A1-44

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Phosphamidon

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier II]

Drinking Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Method 525.2:

•	Add sodium sulfite to empty
sample container to remove
residual chlorine from treated
water samples'51

•	Acidify to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Method 5?5 3 Cto errmtv
sample container):

•	Add 4 mg ascorbic acid to
remove residual chlorine from
treated water samples

•	Add 14 mg trisodium EDTA,
and 400 mg potassium
dihydrogen citrate

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

525.3 (EPA OW)
[Tier I]

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

3520C / 3535A / 8270E

(EPA SW-846)

[Tier I]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier II]

Phosphine

Air

1 (at 0.3 ppm) - 16
L (flow rate = 0.01 -
0.2 L/min)

Sorbent tube (Hg(CN)2 coated
silica gel [300 mg/150 mg])

7 days

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap tubes with
plastic (not rubber) caps. Place tubes in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Phosphine, 2.3, 2.1,
Flammable gas,
Poisonous gas,
UN2199

6002(NIOSH)
[Tier I]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Phosphorus trichloride

Air

11 (at 0.5 ppm) -
100 L (flow rate =
0.05-0.2 L/min)

Bubbler (15 mLH20)

Seal and transfer the bubbler
to container for shipping.

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap each glass
container with bubble wrap. Pack samples
as described in Footnote (4).

Standard carrier
shipping label AND
Phosphorus trichloride,
6.1, Poison, UN1809

6402 (NIOSH)
[Tier I]

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

SAM Companion - Sample Collection Information Document

A1-45

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

Sample Type

Sample Size'1'

Sample Container'2'

Holding Time

Preservation

Packaging Requirements

Shipping Label'3'

Selected Analytical
Methods - SAM 2022*

Pinacolyl methyl phosphonic acid (PMPA) (degradation
product of GD)

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,
n.o.s, 6.1, Poison,
UN3464

TO-10A (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

24 hours

Cool to < 6°C; keep from
freezing

• Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51 (8>

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
compound, toxic, liquid,
n.o.s, 6.1, Poison,
UN3278

D7597-16 (ASTM)

[Tier III - Drinking Water]
[Tier II - Non-Drinking Water]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days

Cool to < 6°C



Standard carrier
shipping label AND
Organophosphorus
compound, toxic, solid,

E2866-12 (ASTM)
[Tier II]



Wipes

1 wipe/100 cm or
1 wipe/ft

Wide-mouth glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

•	Cool to < 6°C

•	Protect from light



UN3464

EPA/600/R-13/224 (EPA)
[Tier II]

Propylene oxide

Air

0.5 (at 100 ppm) - 5
L (flow rate = 0.01 -
0.2 L/min)

Solid sorbent tube (coconut
shell charcoal [100 mg/50 mg])

6	weeks if frozen;

7	days if not frozen

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap tubes with
plastic (not rubber) caps. Place tubes in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Propylene oxide, 3,
Flammable, UN1280.

1612 (NIOSH)
[Tier I]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass VOA vial with
fluoropolymer-lined septum,
filled with no headspace

14 days with
acidification; 7 days if
not acidified

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Adjust to pH < 2 with 1:1 HCI

•	Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Propylene oxide, 3,
Flammable liquid,
UN1280

5030C / 8260D

(EPA SW-846)
[Tier II]



Solid

5-g coring device or
amber VOA vial

AND

1 - 2 oz. glass jar
(if needed to
determine percent
moisture)

Coring device (If coring device
is to be cleaned and reused,
samples can be transferred to
an amber VOA vial.)

AND glass jar (if needed to
determine percent moisture)

14 days if frozen;
48 hours if not frozen

Cool to < 6°C; freeze within 48
hours to < negative (-) 7°C
[Coring tools should not be
frozen below -20°C.]

Wipe outside of coring device or container
clean using a damp, then dry cloth. Place
the coring device or container in a zipper
bag. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Propylene oxide, 3,
Flammable, UN1280

5035A / 8260D

(EPA SW-846)
[Tier II]



Wipes

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

R-33 (VR) [methylphosphonothioic acid, S-[2-
(diethylamino)ethyl]0-2-methylpropyl ester]

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach

TQ-17 (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Cool to < 6°C; keep from
freezing

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before

wipe, taking care not to
obscure labeling.

EPA/600/R-12/653 (EPA)
[Tier II]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

placing in cooler.



EPA/600/R-12/653 (EPA)
[Tier II]



Wipes

1 wipe/100 cm or
1 wipe/ft











EPA/600/R-12/653 (EPA)
[Tier II]

SAM Companion - Sample Collection Information Document

A1-46

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Sarin (GB)

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach

TO-17 (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Cool to < 6°C; keep from
freezing

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before

wipe, taking care not to
obscure labeling.

EPA/600/R-16/115 (EPA)
[Tier I]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

placing in cooler.



EPA/600/R-16/115 (EPA)
[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











EPA/600/R-16/115 (EPA)
[Tier I]

Sodium arsenite (analyze as total arsenic)

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected Alter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Sodium arsenite, solid,
6.1, Poison, UN2027

IO-3.1 /
10-3.4/10-3.5

(EPA ORD)
[Tier 1]



Drinking Water

125 mL

Fluoropolymer, plastic or glass
container

6 months with
acidification. Samples
must be acidified within
14 days of collection.

•	Acidify to pH < 2 with
HN03(7> [If arsenic species are
to be determined, remove
unpresetved aliquot priorto
sample preservation.]

•	Acidified samples can be
cooled to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack

Standard carrier
shipping label AND
Sodium arsenite,
aqueous solutions, 6.1,
Poison, UN 1686

200.7/200.8 (EPA OW)
[Tier 1]



Non-Drinking
Water

125 mL





samples as described in Footnote (4).



3015A /
6010D/ 6020B

(EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

Analyze immediately/ as
soon as possible

Cool to < 6°C



Standard carrier
shipping label AND
Sodium arsenite, solid,
6.1, Poison, UN2027

3050B / 3051A /

6010D /6020B (EPA SW-846)

[Tier I]



Wipes

1 wipe/100 cm or
1 wipe/ft









9102 (NIOSH)/

6010D /6020B (EPA SW-846)

[Tier I]

Sodium azide (analyze as azide ion)

Air

5 L (flow rate = 1
L/min)

Filter / Sorbent tube (PVC or
quartz filter with silica coated
sorbent [150 mg/75 mg])

10 days

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Sodium azide, 6.1,
Poison, UN 1687

ID-211 (OSHA)
[Tier I]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C; keep from
freezing

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).



J. of Forensic Sciences (1998)
43(1): 200-202/

300.1, Rev 1.0 (EPA OW)

[Tier II]



Solid

250 mL

Wide-mouth glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

Until holding time data
are available, analyze
immediately/ as soon as
possible

•	Cool to < 6°C

•	Protect from light

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



J. of Forensic Sciences (1998)
43(1): 200-202/

300.1, Rev 1.0 (EPA OW)

[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

10 days





ID-211 (OSHA)
[Tier I]

SAM Companion - Sample Collection Information Document

A1-47

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Soman (GD)

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach

TQ-17 (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Cool to < 6°C; keep from
freezing

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before

wipe, taking care not to
obscure labeling.

EPA/600/R-16/115 (EPA)
[Tier I]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

placing in cooler.



EPA/600/R-16/115 (EPA)
[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











EPA/600/R-16/115 (EPA)
[Tier I]

Strychnine

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

Standard carrier
shipping label AND
Strychnine, 6.1, Poison,
UN1692

3535A / 8270E

(EPA SW-846)
[Tier I]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C



EPA/600/R-16/114 (EPA)
[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











EPA/600/R-16/114 (EPA)
[Tier II]

Tabun (GA)

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach

TQ-17 (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C; keep from
freezing

• Add sodium thiosulfate to
remove residual chlorine from
treated water samples (5> <10>

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

wipe, taking care not to
obscure labeling.

EPA/600/R-12/653 (EPA)
[Tier II]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C



EPA/600/R-12/653 (EPA)
[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











EPA/600/R-12/653 (EPA)
[Tier II]

SAM Companion - Sample Collection Information Document

A1-48

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Tetraethyl pyrophosphate
(TEPP)

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

TO-10A (EPA ORD)
[Tier II]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

Standard carrier
shipping label AND
Organophosphorus
pesticides, liquid, 6.1,
Poison, UN3018

3511/8270E

(EPA SW-846)
[Tier II]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool samples to < 6°C

Standard carrier
shipping label AND
Organophosphorus
pesticides, solid, 6.1,
Poison, UN2783

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier II]

Tetramethylenedisulfo-tetramine (TETS)

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Pesticides, solid, toxic,
n.o.s, 6.1, Poison,
UN2588

TO-10A (EPA ORD)
[Tier II]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

• Cool to < 6°C; keep from
freezing

For treated water samDles:

Wipe outside of container clean using a
damp, then dry cloth. Seal the vial with non-
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Pesticides, liquid, toxic,
n.o.s, 6.1, Poison,
UN2902

EPA/600/R-16/114 (EPA)
[Tier I]

Preservatives/dechlorinating
agents not required, but the
analyte is not affected by the
following, if added to address
other analytes:

•	Sodium sulfite (5)

•	1.6- 2.0 mg NH4CI

For all other samoles:

• Can be adjusted to pH < 2
with 1:1 HCI, but this is not
required

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Pesticides, solid, toxic,
n.o.s, 6.1, Poison,
UN2588

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm or
1 wipe/ft

EPA/600/R-16/114 (EPA)
[Tier II]

Thallium sulfate (analyze as total thallium)

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Thallium compounds
n.o.s, 6.1, Poison,
UN1707

IO-3.1 /
10-3.4/10-3.5

(EPA ORD)
[Tier 1]

Drinking Water

125 mL

Fluoropolymer, plastic or glass
container

6 months with
acidification. Samples
must be acidified within
14 days of collection.

•	Acidify to pH < 2 with

hno3(7)

•	Acidified samples can be
cooled to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

200.7/200.8 (EPA OW)
[Tier 1]

Non-Drinking
Water

3015A /

6010D /6020B (EPA SW-846)
[Tier I]

Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

Analyze immediately/ as
soon as possible

Cool to < 6°C

3050B / 3051A /

6010D /6020B (EPA SW-846)

[Tier I]

Wipes

1 wipe/100 cm2or
1 wipe/ft2

9102 (NIOSH)/

6010D /6020B (EPA SW-846)

[Tier I]

SAM Companion - Sample Collection Information Document

A1-49

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Thiodiglycol (TDG)
(degradation product of HD)

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Aviation regulated solid,
n.o.s, UN3335

TO-10A (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

24 hours

Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Aviation regulated
liquid, n.o.s, UN3334

D7598-16 (ASTM)

[Tier III - Drinking Water]
[Tier II - Non-Drinking Water]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or

7 days

Cool to < 6°C

Standard carrier
shipping label AND
Aviation regulated solid,

E2787-11 (ASTM)
[Tier II]



Wipes

1 wipe/100 cm2or
1 wipe/ft2

lid







n.o.s, UN3335

E2838-11 (ASTM)
[Tier II]

Thiofanox

Air

240 - 480 L

(flow rate = 0.1 - 1.0

L/min)

Filter/solid sorbent tube (OVS-
2 tube: 13 mm quartz filter,
XAD-2, [270 mg/140 mg])

30 days if frozen; 7 days
at room temperature
(24°C)

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap both ends of the
tube with plastic caps. Place tube in double
plastic bags and wrap with bubble wrap.
Pack samples as described in Footnote
(4).

Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757

5601 (NIOSH)
[Tier III]



Drinking Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Prior to collection, add 400
|jL of ammonium acetate
concentrated stock and 80 jj L
of concentrated sodium
omadine stock to sample
container. [If volumes other
than 40-mL are collected, the
concentration of ammonium
acetate and sodium omadine
in the sample should be 1.5
g/L and 64 mg/L, respectively.]

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

Standard carrier
shipping label AND
Carbamate pesticides,
liquid, toxic, 6.1,
Poison, UN2992

538 (EPA OW)
[Tier I]



Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

•	Prior to collection of treated
water samples, add 10 mg
ascorbic acid to sample
container

•	Acidify to pH < 3.8 with
glacial acetic acid

•	Cool to < 6°C; keep from
freezing





D7645-16 (ASTM)
[Tier II]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

Until holding time data
are available, analyze
immediately/ as soon as
possible

Cool to < 6°C



Standard carrier
shipping label AND
Carbamate pesticides,
solid, toxic, 6.1,
UN2757

3541 / 3545A (EPA SW-846) /
D7645-16 (ASTM)

[Tier III]



Wipes

1 wipe/100 cm2or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days







3570 / 8290A Appendix A

(EPA SW-846)/

D7645-16 (ASTM)

[Tier III]

SAM Companion - Sample Collection Information Document

A1-50

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

1,4-Thioxane

(degradation product of HD)

Air

Not of concern in this sample type

NA

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

• Cool to < 6°C; keep from
freezing

For treated water samnles-

Wipe outside of container clean using a
damp, then dry cloth. Seal the vial with non
reactive tape or film (e.g., fluoropolymer-
based), and wrap with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Flammable Liquids,
n.o.s, 3, Flammable,
UN1993

EPA/600/R-16/114 (EPA)
[Tier II]

•	Add sodium sulfite to remove
residual chlorine'51 OR

•	Add 1.6 - 2.0 mg NH4CI

For all other samoles:

• Adjust to pH < 2 with 1:1 HCI

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

14 days

Cool to < 6°C

Standard carrier
shipping label AND
Flammable Solid, n.o.s,
3, Flammable, UN1325

EPA/600/R-16/114 (EPA)
[Tier II]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

EPA/600/R-16/114 (EPA)
[Tier II]

Titanium tetrachloride (analyze as total titanium)

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA

Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

Analyze immediately/ as
soon as possible

Cool to < 6°C

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Titanium tetrachloride,
8, Corrosive, UN1838

3050B / 3051A /

6010D /6020B (EPA SW-846)

[Tier I]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth fluoropolymer or
glass container with
fluoropolymer-lined lid

3051A /

6010D /6020B (EPA SW-846)
[Tier III]

Triethanolamine (TEA) (degradation product of HN-3)

Air

0.5-300 L (flow
rate =0.5-1 L/min)

Impinger (15 mL 2 mM
hexanesulfonic acid)

[Impinger contents are
transferred into VOA vial for

21 days

•	Cool to < 6°C

•	Protect from light

Wipe outside of vial clean using a damp,
then dry cloth. Seal the vial with non-
reactive tape or film, and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Ethanolamine solutions,
8, Corrosive, UN2491

3509 (NIOSH)
[Tier II]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

D7599-16 (ASTM)

[Tier III - Drinking Water]
[Tier II - Non-Drinking Water]

Solid

250 mL

Wide-mouth amber glass,
fluoropolymer or polypropylene
container

14 days

Cool to < 6°C

3541 /3545A (EPA SW-846) /
EPA/600/R-11/123

(EPA/CDC)

[Tier III]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth amber glass,
fluoropolymer or polypropylene
container

28 days

EPA/600/R-11/123

(EPA/CDC)

[Tier II]

SAM Companion - Sample Collection Information Document

A1-51

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.implo Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Trimethyl phosphite

Air

240 - 7200 L
(flow rate = 1-5
L/min)

Sorbent cartridge containing
PUF or PUF in combination
with other solid sorbent

7 days

Cool to < 6°C

Remove PUF cartridge from sampler, wrap
with aluminum foil used to store cartridge,
and place in a sealed container. Place
container in double plastic bags and wrap
with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Trimethyl phosphite, 3,
Flammable, UN2329

TO-10A (EPA ORD)
[Tier III]



Drinking Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Non-Drinking
Water

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon

Standard carrier
shipping label AND
Trimethyl phosphite, 3,
Flammable, UN2329

3541 / 3545A / 8270E

(EPA SW-846)

[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2







plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.



3570 / 8290A Appendix A /
8270E (EPA SW-846)

[Tier III]

1,3,5-Trinitrobenzene
(1,3,5-TNB)

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene mixtures,
1.1, Explosive,
UN0388(9)

3535A / 8330B

(EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C





8330B (EPA SW-846)
[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days







3570 / 8290A Appendix A /
8330B (EPA SW-846)

[Tier I]

2,4,6-Trinitrotoluene
(2,4,6-TNT)

Air

For the purposes of this document, this analyte is not considered to be a concern in this sample type

NA



Drinking Water

Non-Drinking
Water

1 L

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

•	Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

Standard carrier
shipping label AND
Trinitrobenzene and
trinitrotoluene mixtures,
1.1, Explosive,
UN0388(9>

3535A / 8330B

(EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

14 days

Cool to < 6°C





8330B (EPA SW-846)
[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or
lid

7 days







3570 / 8290A Appendix A /
8330B (EPA SW-846)

[Tier I]

SAM Companion - Sample Collection Information Document

A1-52

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Analyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

Vanadium pentoxide (analyze as total vanadium)

Air

Up to 1700 m3

Filter (quartz, silica, or
cellulose fiber)

6 months

Cool to < 6°C

Place filter in protective covering. Place
protected filter in double plastic bags, and
wrap with bubble wrap. Pack samples as
described in Footnote (4).

Standard carrier
shipping label AND
Vanadium pentoxide,
6.1, Poison, UN2862

IO-3.11

IO-3.4 / IO-3.5 (EPA ORD)
[Tier I]

Drinking Water

125 mL

Fluoropolymer, plastic or glass
container

6 months with
acidification. Samples
must be acidified within
14 days of collection.

•	Acidify to pH < 2 with

hno3(7)

•	Acidified samples can be
cooled to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).

200.7/ 200.8 (EPA OW)
[Tier I]

Non-Drinking
Water

3015A /

6010D /6020B (EPA SW-846)
[Tier I]

Solid

250 mL

Wide-mouth fluoropolymer,
plastic or glass container

Analyze immediately/ as
soon as possible

Cool to < 6°C

3050B / 3051A /

6010D /6020B (EPA SW-846)

[Tier I]

Wipes

1 wipe/100 cm2or
1 wipe/ft2

9102 (NIOSH)/

6010D /6020B (EPA SW-846)

[Tier I]

VE [Phosphonothioic acid, ethyl-, S-(2-(diethylamino)ethyl) 0-
ethyl ester]

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach
wipe, taking care not to
obscure labeling.

TO-17 (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Cool to < 6°C; keep from
freezing

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

EPA/600/R-16/116 (EPA)
[Tier III]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

EPA/600/R-16/116 (EPA)
[Tier III]

Wipes

1 wipe/100 cm2 or
1 wipe/ft2

EPA/600/R-16/116 (EPA)
[Tier III]

VG [Phosphorothioic acid, S-(2-(diethylamino)ethyl) 0,0-
diethyl ester]

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach
wipe, taking care not to
obscure labeling.

TO-17 (EPA ORD)
[Tier III]

Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Cool to < 6°C; keep from
freezing

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before
placing in cooler.

EPA/600/R-16/116 (EPA)
[Tier III]

Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

EPA/600/R-16/116 (EPA)
[Tier III]

Wipes

1 wipe/100 cm2 or
1 wipe/ft

EPA/600/R-16/116 (EPA)
[Tier III]

SAM Companion - Sample Collection Information Document

A1-53

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

An.ilyte

S.imple Type

S.implo Size

S.implo Contiinor'

Holding Time

Preservation

P.ick.iging Requirements

Shipping L.ibel

Selected An.ilytic.il
Methods - SAM 2022"

VM [Phosphonothioic acid, methyl-, S-(2-(diethylamino)ethyl)
O-ethyl ester]

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach

TO-17 (EPA ORD)
[Tier III]



Drinking Water

Non-Drinking
Water

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

•	Cool to < 6°C; keep from
freezing

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist
punctures, then into metal can before

wipe, taking care not to
obscure labeling.

EPA/600/R-16/116 (EPA)
[Tier III]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

placing in cooler.



EPA/600/R-16/116 (EPA)
[Tier III]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2











EPA/600/R-16/116 (EPA)
[Tier III]

VX [0-ethyl-S-(2-diisopropylaminoethyl) methyl-
phosphonothiolate]

Air

1 - 4 L (flow rate =
16-67 mL/min)

Stainless steel, 3.5-inch long
tubes with a 6-cm sorbent bed
and 1/4-inch o.d.

7 days

Cool to < 6°C

Wrap tube in aluminum foil, and place in a
clean sealed container. Place container in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Consult laboratory
and/or shipping vendor
on specifics regarding
shipping label. Wipe
outer surface of
package with a bleach

TQ-17 (EPA ORD)
[Tier II]



Drinking Water

Non-Drinking
Water

40 mL



7 days

•	Cool to < 6°C; keep from
freezing

•	Add sodium thiosulfate to
remove residual chlorine from
treated water samples'51

Wipe outside of container clean using a
bleach wipe, taking care not to affect any
labeling. Make sure bleach is removed and
the outside of container is dry, then wrap
container with bubble wrap. Place in gallon
plastic (polypropylene or polyethylene) bag
with zipper lock and durability to resist

wipe, taking care not to
obscure labeling.

EPA/600/R-16/116 (EPA)
[Tier I]



Solid

40 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid

7 days

Cool to < 6°C

placing in cooler.



EPA/600/R-16/116 (EPA)
[Tier II]



Wipes

1 wipe/100 cm2 or
1 wipe/ft











EPA/600/R-16/116 (EPA)
[Tier II]

White phosphorus

Air

5 L (at 0.1 mg/m3) -
100 L (flow rate =
0.01 -0.2 L/min)

Solid sorbent tube (Tenax GC
[100 mg/50 mg])

7 days

Cool to < 6°C

Wipe outside of sorbent tube clean using a
damp, then dry cloth. Cap tubes with
plastic (not rubber) caps. Place tubes in
double plastic bags and wrap with bubble
wrap. Pack samples as described in
Footnote (4).

Standard carrier
shipping label AND
Phosphorus, white (dry
or wetted), 4.2,
Spontaneously
combustible, 6.1,
Poison, UN1381

7905 (NIOSH)
[Tier I]



Drinking Water

Non-Drinking
Water

500 mL

Amber glass or fluoropolymer
container with fluoropolymer-
lined septum or lid; fill to
overflowing to eliminate
headspace

5 days

Cool to < 6°C; keep from
freezing

Wipe outside of container clean using a
damp, then dry cloth. Seal the container
with non-reactive tape or film (e.g.,
fluoropolymer-based). Wrap glass
containers with bubble wrap. Pack
samples as described in Footnote (4).



7580 (EPA SW-846)
[Tier I]



Solid

250 mL

Wide-mouth amber glass or
fluoropolymer container with
fluoropolymer-lined septum or

Samples can be held
indefinitely if sealed
tightly to eliminate

Cool to < 6°C





7580 (EPA SW-846)
[Tier I]



Wipes

1 wipe/100 cm2 or
1 wipe/ft2

lid

moisture loss







3570 / 8290A Appendix A /
7580 (EPA SW-846)

[Tier III]

SAM Companion - Sample Collection Information Document

A1-54

April 2023

-------
Sample Collection Information Document - Attachment A

April 2023

Footnotes:

NA Not applicable

* EPA's SAM lists the analytical methods that have been selected by multi-agency technical work groups for use by laboratories that are tasked with sample analysis following a contamination incident. The
method tiers listed reflect the extent of method validation and indicate the level of method usability for the specific analyte and sample type. These tiers are defined in the Chemical Methods Query on the
Selected Analytical Methods for Environmental Remediation and Recovery website:
https://www.epa.aov/esam/selected-analvtical-methods-environmental-remediation-and-recoverv-sam

(1) Sample sizes are based on the minimum amount needed for analysis of a single sample. Additional sample amounts may be requested or needed for laboratory quality control analyses (e.g., duplicates, matrix
spikes) or for measurement of low analyte concentrations. It is also recommended that additional sample(s) be collected in case of the need for reanalysis due to sample spillage or unforeseen analytical
difficulties. Where possible, sample sizes are based on specifications included in the selected analytical methods.

• Wipe areas may need to be adjusted based on anticipated analyte concentration. Wipe sample areas are based on U.S. Office of Research and Development, "A Literature Review of Wipe Sampling
Methods for Chemical Warfare Agents and Toxic Industrial Chemicals," EPA/600/R-07/004 January 2007.

• Solid samples should be collected into containers without packing.

(2) Use of "certified clean" containers is preferred. Otherwise, use glassware cleaning instructions found in Chapter 3 of SW-846. Rinse with detergent, followed by tap water, 1:1 nitric acid:tap water, 1:1
hydrochloric acid:tap water, and reagent water. Prior to use, wash vials and septa with detergent and rinse with tap and distilled water. Allow the vials and septa to air dry at room temperature, place in a 105°C
oven for 1 hr, then remove and allow to cool in an area known to be free of organics.

(3) Environmental samples are shipped as nonhazardous cargo if they do not exhibit one of the following U.S. DOT hazard classes: explosive, corrosive, flammable, or poisonous. Only a standard shipping carrier's
label is needed in these cases. If any sample is listed under one of these classes, DOT labeling is required. The DOT label must indicate, PSN (49 CFR 172.301), the UN Number (49 CFR 172.301), appropriate
hazard class and descriptor (49 CFR 172.101), and "Limited Quantity" when a quantity exception is used (49 CFR 172.101). When the quantity limit for a given substance is exceeded, the phrase "Limited
Quantity" should be excluded from the label. Note that there are no "exceptions" for substances belonging to DOT Division 1.1, Division 2.1, Division 2.3 or Division 4.2. Exception quantities include:

• Exception for Class 3, flammable and combustible liquids: Ensure total net quantity of Class 3 substances does not exceed 1 L (0.3 gal) (49 CFR 173.150)

• Exception for Division 5.1, oxidizers and Class 9, miscellaneous hazardous materials (see 49 CFR 173.127 for packing group definitions), Packing group II: Ensure the total net quantity does not
exceed 1.0 L (0.3 gallons) of liquid or 1.0 kg (2.2 lbs) of solid (49 CFR 173.152); Packing group III: Ensure the total net quantity does not exceed 5 L (1.3 gallons) of liquid or 5.0 kg (11 lbs) of solid
(49 CFR 173.152)

• Exception for Division 6.1, poisonous liquids: Ensure the total net quantity of Class 6.1 liquids does not exceed 4 L (1 gal) of liquid or 5 kg (11 lbs) of solid (49 CFR 173.153)

• Exception for Class 8, corrosive materials: Ensure total net quantity of Class 8 materials does not exceed 5 L (1.3 gal) of liquid or 5 kg (11 lbs) of solid (49 CFR 173.154)

• Shipment of samples containing reactive levels (>12%) of energetic material residues using domestic carriers is prohibited. If samples are known or suspected to contain >12% of energetic material
residues, ship and label according to DOT requirements for secondary explosive materials.

(4) Pack sample transport containers outside the contaminated area. Samples must be packed in a manner that protects the integrity of the samples and provides temperature conditions required for sample
preservation. Samples should be surrounded by shock- and water-absorbent packing materials or ice (if required for preservation) and shipped in a cooler to ensure sample temperatures do not exceed
preservation requirements. If the target analytes are unknown, samples should be maintained at < 6°C, but above freezing. Ice should be placed in separate plastic bags or cold packs should be used to avoid
leakage, and the bags placed around, among, and on top of the sample containers.

(5) Sodium thiosulfate or sodium sulfite should be added to treated drinking water or wastewater samples containing residual chlorine. These dechlorinating agents can be added either prior to or after sample
collection, but before sample preservation.

• For sodium thiosulfate, an amount equivalent to the following is recommended: 3 mg/40-mL sample, or 3 mg/5 mg chlorine residual.

• For sodium sulfite, an amount equivalent to the following is recommended: for a 1-L sample, add 40 - 50 mg sodium sulfite (1.6-2 mg/40-mL sample), either prior to or after collecting the sample.

(6) This method applies to ambient concentrations of VOCs above 0.5 ppbv and typically requires VOC enrichment by concentrating up to 1 L of sample volume; however, when using current technologies,
quantifications of approximately 100 pptv have been achieved with 0.5-L sample volumes.

(7) If sample collectors are not able to preserve samples in the field (e.g., acid preservatives are not available), the samples may be preserved in the laboratory. If preserved in the laboratory, however, samples
must be held for at least 24 hours prior to analysis.

(8) Laboratory data show that EPA Method 538 analysis of EA2192 is not impacted by the presence of dechlorinating agent; data are not currently available regarding potential impacts on the analysis of similar
compounds when using the selected SAM method.

(9) Samples containing secondary explosives at concentrations less than 10% by weight (100,000 mg/kg) or primary explosives at concentrations less than 2% (20,000 mg/kg) can be sent as normal environmental
samples. Consult laboratory on specifics regarding shipping label and shipping requirements for samples with concentrations greater than these amounts.

(10) Laboratory data show that Method EPA/600/R-16/115 analysis of Sarin and Soman is not impacted by the presence of dechlorinating agent; data are not currently available regarding impacts on the analysis of
Tabun when using this method.

SAM Companion - Sample Collection Information Document

A1-55

April 2023

-------
Attachment B1

Sample Collection Information for the
Environmental Samples, Radiochemical
Analytes and Methods Listed in SAM 2022

[Note: The acronyms and abbreviations used in this
attachment are listed and defined in the beginning of the

report.]

-------
Sample Collection Information Document - Attachment B1
April 2023

Attachment B1: Sample Collection Information for the Environmental Samples, Radiochemical Analytes and Methods Listed in SAM 2022

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time14'

Sample Preservation*4*

Analytical Method'5'

Gross Alpha

Air Filter

1 filter (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

No preservation required

FRMAC, Vol. 2, pg. 33 (DOE)



Aqueous & Liquid

2 - 4 L (solids limited)

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

Preserve sample with
HN03 or HCI to pH < 2 (6)

7110 B (SM)



Drinking Water

2 - 4 L (solids limited)

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

Preserve sample with
HN03 or HCI to pH < 2 (6)

900.0 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months

No preservation required

AP1 (ORISE)



Surface Wipes

1 wipe (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

No preservation required

FRMAC, Vol. 2, pg. 33 (DOE)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months

No preservation required

AP1 (ORISE)

Gross Beta

Air Filter

1 filter (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

No preservation required

FRMAC, Vol. 2, pg. 33 (DOE)



Aqueous & Liquid

2 - 4 L (solids limited)

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

Preserve sample with
HNOs or HCI to pH < 2 (6)

7110 B(SM)



Drinking Water

2 - 4 L (solids limited)

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

Preserve sample with
HN03 or HCI to pH < 2 (6)

900.0 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar,
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months

No preservation required

AP1 (ORISE)



Surface Wipes

1 wipe (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

No preservation required

FRMAC, Vol. 2, pg. 33 (DOE)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months

No preservation required

AP1 (ORISE)

SAM Companion - Sample Collection Information Document

B1 -1

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Gamma
Select Mixed

Air Filter

1 filter (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

No preservation required

Ga-01-R (HASL-300)

Fission Products

Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

Preserve sample with
HN03 or HCI to pH < 2 (6)

Ga-01-R (HASL-300)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

Preserve sample with
HN03 or HCI to pH < 2 (6)

901.1 (EPA)



Soil & Sediment

100- 1,000 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months

No preservation required

Ga-01-R (HASL-300)



Surface Wipes

1 wipe (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

No preservation required

Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months

No preservation required

Ga-01-R (HASL-300)

Total Activity
Screening

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

No preservation required

Preparation of Samples for Total Activity
Screening (Y-12)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

No preservation required

Preparation of Samples for Total Activity
Screening (Y-12)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

No preservation required

Preparation of Samples for Total Activity
Screening (Y-12)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months

No preservation required. IfTc-
99 is to be assessed, a separate
sample will be necessary.

Preparation of Samples for Total Activity
Screening (Y-12)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

No preservation required. If Tc-
99 is to be assessed, a separate
sample will be necessary.

Preparation of Samples for Total Activity
Screening (Y-12)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months

No preservation required. If Tc-
99 is to be assessed, a separate
sample will be necessary.

Preparation of Samples for Total Activity
Screening (Y-12)

SAM Companion - Sample Collection Information Document

B1 -2

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Actinium-225

Air Filter

1 filter (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Ac-225 half life = 9.92 days)

No preservation required

Qualitative: FRMAC. Vol. 2. Da.33 (DOE)

Confirmatory: Determination of Actinium-
225 in Geological Samples (Eichrom)



Aqueous & Liquid

2 - 4 L (solids limited)

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Ac-225 half life = 9.92 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative: 711 OB CSM)

Confirmatory: Determination of Actinium-
225 in Water Samples (Eichrom)



Drinking Water

2 - 4 L (solids limited)

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Ac-225 half life = 9.92 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative: 900.0 (EPA)

Confirmatory: Determination of Actinium-
225 in Water Samples (Eichrom)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Ac-225 half life = 9.92 days)

No preservation required

Qualitative: AP1 (ORISE)

Confirmatory: Determination of Actinium-
225 in Geological Samples (Eichrom)



Surface Wipes

1 wipe (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Ac-225 half life = 9.92 days)

No preservation required

Qualitative: FRMAC. Vol. 2. Da.33 (DOE)

Confirmatory: Determination of Actinium-
225 in Geological Samples (Eichrom)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(Ac-225 half life = 9.92 days)

No preservation required

Qualitative: AP1 (ORISE)

Confirmatory: Determination of Actinium-
225 in Geological Samples (Eichrom)

SAM Companion - Sample Collection Information Document

B1 -3

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Americium-241
(alpha)

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Arn-241 half life = 432 years)

No preservation required

Qualitative: Raoid Methods for Acid or
Fusion Digestion, EPA 402-R-12-009 and
EPA 402-R-12-008 (EPA)

Confirmatory: Am-04-RC (HASL-300)

Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Am-241 half life = 432 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative: D3084-20 fASTM)
Confirmatory: Am-04-RC (HASL-300)

Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Am-241 half life = 432 years)

Qualitative: No samole
preservation is required if sample
is delivered to the laboratory
within 3 days of collection. If the
sample is to be held for more
than 3 days, add concentrated
HNO3 to achieve a pH < 2.

Confirmatory: Preserve sample
with HNOjorHCIto pH < 2 (6)

Qualitative: Raoid Radiochemical Method
for Am-241, EPA 402-R-10-001a (EPA)

Confirmatory: Am-04-RC CHASL-300')

Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months
(Am-241 half life = 432 years)

No preservation required

Qualitative: Raoid Method for Fusion of
Soil and Soil-Related Matrices, EPA 600-
R-12-636, EPA 600-R-12-637 and EPA
600-R-12-638 (EPA)

Confirmatory: Am-01-RC (HASL-300)

Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Am-241 half life = 432 years)

No preservation required

Qualitative: Raoid Methods for Acid or
Fusion Digestion, EPA 402-R-12-009 and
EPA 402-R-12-008 (EPA)

Confirmatory: Am-04-RC (HASL-300)

Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(Am-241 half life = 432 years)

No preservation required

Qualitative: Actinides and
Sr-89/90 in Vegetation, SRS Manual
L3.23-10055 (DOE SRS)

Confirmatory: Am-06-RC (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -4

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Americium-241
(gamma)

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Am-241 half life = 432 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Am-241 half life = 432 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
7120 (SM)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Am-241 half life = 432 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months
(Am-241 half life = 432 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Am-241 half life = 432 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(Am-241 half life = 432 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -5

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Californium-252

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Cf-252 half life = 2.64 years)

No preservation required

Qualitative: D3084-20 CASTM)

Confirmatory: Raoid Radiochemical
Method for Californium-252, EPA 402-
S17-003 (EPA)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Cf-252 half life = 2.64 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative: D3084-20 fASTM)

Confirmatory: Raoid Radiochemical
Method for Californium-252, EPA 402-
S17-003 (EPA)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Cf-252 half life = 2.64 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative: D3084-20 CASTM)

Confirmatory: Raoid Radiochemical
Method for Californium-252, EPA 402-
S17-003 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar,
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months
(Cf-252 half life = 2.64 years)

No preservation required

Qualitative: D3084-20 CASTM)

Confirmatory: Rapid Radiochemical
Method for Californium-252, EPA 402-
S17-003 (EPA)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Cf-252 half life = 2.64 years)

No preservation required

Qualitative: D3084-20 (ASTM)

Confirmatory: Raoid Radiochemical
Method for Californium-252, EPA 402-
S17-003 (EPA)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(Cf-252 half life = 2.64 years)

No preservation required

Qualitative: D3084-20 CASTM)

Confirmatory:

Am-06-RC (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -6

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Cesium-137

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Cs-137 half life = 30.17 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Cs-137 half life = 30.17 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
7120 (SM)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Cs-137 half life = 30.17 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months
(Cs-137 half life = 30.17 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Cs-137 half life = 30.17 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(Cs-137 half life = 30.17 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

Cobalt-60

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Co-60 half life = 5.27 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Co-60 half life = 5.27 years)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
7120 (SM)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Co-60 half life = 5.27 years)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months
(Co-60 half life = 5.27 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Co-60 half life = 5.27 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(Co-60 half life = 5.27 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -7

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Curium-244

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Cm-244 half life = 18.1 years)

No preservation required

Qualitative: D3084-20 CASTM)

Confirmatory: Raoid Radiochemical
Method for Curium-244 in Air Particulate
Filters, Swipes and Soil, EPA 402-S17-
004 (EPA)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Cm-244 half life = 18.1 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative: D3084-20 CASTM)

Confirmatory: Raoid Radiochemical
Method for Curium-244 in Water
Samples, EPA 402-S17-001 (EPA)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Cm-244 half life = 18.1 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative: D3084-20 CASTM)

Confirmatory: Raoid Radiochemical
Method for Curium-244 in Water
Samples, EPA 402-S17-001 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months
(Cm-244 half life = 18.1 years)

No preservation required

Qualitative: D3084-20 CASTM)

Confirmatory: Raoid Radiochemical
Method for Curium-244 in Air Particulate
Filters, Swipes and Soil, EPA 402-S17-
004 (EPA)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Cm-244 half life = 18.1 years)

No preservation required

Qualitative: D3084-20 CASTM)

Confirmatory: Raoid Radiochemical
Method for Curium-244 in Air Particulate
Filters, Swipes and Soil, EPA 402-S17-
004 (EPA)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(Cm-244 half life = 18.1 years)

No preservation required

Qualitative: D3084-20 CASTM)
Confirmatory: Am-06-RC (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -8

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Europium-154

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Eu-154 half life = 8.59 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Eu-154 half life = 8.59 years)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
7120 (SM)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Eu-154 half life = 8.59 years)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months
(Eu-154 half life = 8.59 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Eu-154 half life = 8.59 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(Eu-154 half life = 8.59 years)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

Gallium-68

Air Filter

1 filter (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Ga-68 half life = 68 minutes)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Ga-68 half life = 68 minutes)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Ga-68 half life = 68 minutes)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100- 1,000 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Ga-68 half life = 68 minutes)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Ga-68 half life = 68 minutes)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(Ga-68 half life = 68 minutes)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -9

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Germanium-68

Air Filter

1 filter (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Ge-68 half life = 271 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Ge-68 half life = 271 days)

Preserve sample with
HN03 or HCI topH <2 (6)

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Ge-68 half life = 271 days)

Preserve sample with
HN03 or HCI to pH<2'6'

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100- 1,000 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months
(Ge-68 half life = 271 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe (limited to
counting geometry size)

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Ge-68 half life = 271 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(Ge-68 half life = 271 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

lndium-111

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(ln-111 half life = 2.8 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Borosilicate glass small-mouth bottle

Maximum 6 months'7'
(ln-111 half life = 2.8 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Drinking Water

2 - 4 L

Borosilicate glass small-mouth bottle

Maximum 6 months'7'
(ln-111 half life = 2.8 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Borosilicate glass wide-mouth jar

Maximum 6 months'7'
(ln-111 half life = 2.8 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(ln-111 half life = 2.8 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(ln-111 half life = 2.8 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -10

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

lodine-125

Air Filter

1 charcoal cartridge

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(1-125 half life = 59.4 days)

No preservation required

Qualitative and Confirmatory:
Procedure #9(8)(ORISE)



Aqueous & Liquid

2 - 4 L

Borosilicate glass small-mouth bottle

Maximum 6 months'7'
(1-125 half life = 59.4 days)

No preservation required

Qualitative and Confirmatory:
Procedure #9(ORISE)



Drinking Water

2 - 4 L

Borosilicate glass small-mouth bottle

Maximum 6 months'7'
(1-125 half life = 59.4 days)

No preservation required

Qualitative and Confirmatory:
Procedure #9(ORISE)



Soil & Sediment

100 g

Borosilicate glass wide-mouth jar

Maximum 6 months'7'
(1-125 half life = 59.4 days)

No preservation required.
1-125 will volatilize with heat;
store samples at room
temperature if stored before
shipping.

Qualitative and Confirmatory:
Procedure #9(ORISE)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months*7'
(1-125 half life = 59.4 days)

No preservation required

Qualitative and Confirmatory:
Procedure #9(ORISE)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(1-125 half life = 59.4 days)

No preservation required.
1-125 will volatilize with heat;
store samples at room
temperature if stored before
shipping.

Qualitative and Confirmatory:
Procedure #9(ORISE)

SAM Companion - Sample Collection Information Document

B1 -11

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

lodine-131

Air Filter

Charcoal or silver
zeolite cartridge

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(1-131 half life = 8.03 days)

No preservation required

Qualitative and Confirmatory:

Ga-01-R(8) (HASL-300)

Aqueous & Liquid

2 - 4 L

Borosilicate glass small-mouth bottle

Maximum 6 months'7'
(1-131 half life = 8.03 days)

No preservation required

Qualitative and Confirmatory:

Ga-01-R (HASL-300)

Drinking Water

2 - 4 L

Borosilicate glass small-mouth bottle

Maximum 6 months'7'
(1-131 half life = 8.03 days)

No preservation required
(Sample acidification
recommended in Method 901.1
should not be applied to samples
collected for analysis of 1-131)

Qualitative and Confirmatory:

901.1 (EPA)

Soil & Sediment

100 g

Borosilicate glass wide-mouth jar

Maximum 6 months'7'
(1-131 half life = 8.03 days)

No preservation required.
1-131 will volatilize with heat;
store samples at room
temperature if stored before
shipping.

Qualitative and Confirmatory:

Ga-01-R (HASL-300)

Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(1-131 half life = 8.03 days)

No preservation required

Qualitative and Confirmatory:

Ga-01-R (HASL-300)

Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(1-131 half life = 8.03 days)

No preservation required.
1-131 will volatilize with heat;
store samples at room
temperature if stored before
shipping.

Qualitative and Confirmatory:

Ga-01-R (HASL-300)

lridium-192

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(lr-192 half life = 74 days)

No preservation required

Qualitative and Confirmatory:

Ga-01-R (HASL-300)

Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(lr-192 half life = 74 days)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative and Confirmatory:

7120 (SM)

Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(lr-192 half life = 74 days)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative and Confirmatory:

901.1 (EPA)

Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(lr-192 half life = 74 days)

No preservation required

Qualitative and Confirmatory:

Ga-01-R (HASL-300)

Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(lr-192 half life = 74 days)

No preservation required

Qualitative and Confirmatory:

Ga-01-R (HASL-300)

Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(lr-192 half life = 74 days)

No preservation required

Qualitative and Confirmatory:

Ga-01-R (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -12

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Molybdenum-99

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Mo-99 half life = 65.94 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Mo-99 half life = 65.94 hours)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Mo-99 half life = 65.94 hours)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Mo-99 half life = 65.94 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Mo-99 half life = 65.94 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(Mo-99 half life = 65.94 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

Neptunium-237

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

(Np-237 half life = 2.14 million years)

No preservation required

Qualitative and Confirmatory:
SOP for Actinides in Environmental
Matrices (EPA-NAREL)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

(Np-237 half life = 2.14 million years)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
SOP for Actinides in Environmental
Matrices (EPA-NAREL)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

(Np-237 half life = 2.14 million years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
907.0 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months

(Np-237 half life = 2.14 million years)

No preservation required

Qualitative and Confirmatory:
SOP for Actinides in Environmental
Matrices (EPA-NAREL)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

(Np-237 half life = 2.14 million years)

No preservation required

Qualitative and Confirmatory:
SOP for Actinides in Environmental
Matrices (EPA-NAREL)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months

(Np-237 half life = 2.14 million years)

No preservation required

Qualitative and Confirmatory:
SOP for Actinides in Environmental
Matrices (EPA-NAREL)

SAM Companion - Sample Collection Information Document

B1 -13

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Neptunium-239

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Np-239 half life = 2.4 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Np-239 half life = 2.4 days)

Preserve sample with
HNOs or HCI to pH < 2 (6)

Qualitative and Confirmatory:
7120 (SM)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Np-239 half life = 2.4 days)

Preserve sample with
HNOs or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Np-239 half life = 2.4 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Np-239 half life = 2.4 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(Np-239 half life = 2.4 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -14

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Phosphorus-32

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(P-32 half life = 14.26 days)

No preservation required

Qualitative and Confirmatory:
RESL P-2 (DOE)

Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(P-32 half life = 14.26 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
R4-73-014 (EPA)

Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(P-32 half life = 14.26 days)

Qualitative: No samole
preservation is required if sample
is delivered to the laboratory
within 3 days of sampling
date/time. If the sample is to be
held for more than 3 days, add
concentrated HNG3 to achieve a
pH < 2.

Confirmatory: Preserve samole
with HNOs or HCI to pH < 2 (6)

Qualitative: Raoid Radiochemical Method
for P-32 in Water, EPA/600/R-11/181
(EPA)

Confirmatory: R4-73-014 (EPA1)

Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(P-32 half life = 14.26 days)

No preservation required

Qualitative and Confirmatory:
RESL P-2 (DOE)

Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(P-32 half life = 14.26 days)

No preservation required

Qualitative and Confirmatory:
RESL P-2 (DOE)

Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months*7'
(P-32 half life = 14.26 days)

No preservation required

Qualitative and Confirmatory:
RESL P-2 (DOE)

SAM Companion - Sample Collection Information Document

B1 -15

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Plutonium-238
Plutonium-239

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

(Pu-238 half life = 87.7 years)
(Pu-239 half life = 24,100 years)

No preservation required

Qualitative: Rapid Methods for Acid or
Fusion Digestion, EPA 402-R-12-009 and
EPA 402-R-12-008 (EPA)

Confirmatory: SOP for Actinides in
Environmental Matrices (EPA-NAREL)

Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

(Pu-238 half life = 87.7 years)
(Pu-239 half life = 24,100 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative: D3084-20 CASTM)

Confirmatory: SOP for Actinides in
Environmental Matrices (EPA-NAREL)

Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

(Pu-238 half life = 87.7 years)
(Pu-239 half life = 24,100 years)

Qualitative: No sample
preservation is required if sample
is delivered to the laboratory
within 3 days of collection. If the
sample is to be held for more
than 3 days, add concentrated
HNO3 to achieve a pH < 2.

Confirmatory: Preserve sample
with HNO3 or HCI to pH < 2 (6)

Qualitative: Rapid Radiochemical Method
for Pu, EPA 402-R-10-001b (EPA)

Confirmatory: EMSL-33 (EPA)

Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months

(Pu-238 half life = 87.7 years)
(Pu-239 half life = 24,100 years)

No preservation required

Qualitative: Rapid Method for Fusion of
Soil and Soil-Related Matrices, EPA 600-
R-12-636, EPA 600-R-12-637 and EPA
600-R-12-638 (EPA)

Confirmatory: SOP for Actinides in
Environmental Matrices (EPA-NAREL)

Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

(Pu-238 half life = 87.7 years)
(Pu-239 half life = 24,100 years)

No preservation required

Qualitative: Rapid Methods for Acid or
Fusion Digestion, EPA 402-R-12-009 and
EPA 402-R-12-008 (EPA)

Confirmatory: SOP for Actinides in
Environmental Matrices (EPA-NAREL)

Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months

(Pu-238 half life = 87.7 years)
(Pu-239 half life = 24,100 years)

No preservation required

Qualitative: Actinides and
Sr-89/90 in Vegetation, SRS Manual
L3.23-10055 (DOE SRS)

Confirmatory: Am-06-RC (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -16

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the

container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Polonium-210

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Po-210 half life = 138 days)

No preservation required

Qualitative and Confirmatory:
Method 111 (EPA)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Po-210 half life = 138 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
Po-02-RC (HASL-300)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Po-210 half life = 138 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
Po-02-RC (HASL-300)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Po-210 half life = 138 days)

No preservation required

Qualitative and Confirmatory:
Po-02-RC (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Po-210 half life = 138 days)

No preservation required

Qualitative and Confirmatory:
Method 111 (EPA)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months*7'
(Po-210 half life = 138 days)

No preservation required

Qualitative and Confirmatory:
Po-02-RC (HASL-300)

Radium-223

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Ra-223 half life = 11.4 days)

No preservation required

Qualitative and Confirmatory:

Rapid Radiochemical Method
for Ra-226, EPA 402-R-10-001c (EPA)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Ra-223 half life = 11.4 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:

Rapid Radiochemical Method
for Ra-226, EPA 402-R-10-001c (EPA)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Ra-223 half life = 11.4 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:

Rapid Radiochemical Method
for Ra-226, EPA 402-R-10-001c (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Ra-223 half life = 11.4 days)

No preservation required

Qualitative and Confirmatory:

Rapid Radiochemical Method
for Ra-226, EPA 402-R-10-001c (EPA)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Ra-223 half life = 11.4 days)

No preservation required

Qualitative and Confirmatory:

Rapid Radiochemical Method
for Ra-226, EPA 402-R-10-001c (EPA)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(Ra-223 half life = 11.4 days)

No preservation required

Qualitative and Confirmatory:

Rapid Radiochemical Method
for Ra-226, EPA 402-R-10-001c (EPA)

SAM Companion - Sample Collection Information Document

B1 -17

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Radium-226

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Ra-226 half life = 1,600 years)

No preservation required

Qualitative: Raoid Methods for Acid or
Fusion Digestion, EPA 402-R-12-009 and
EPA 402-R-12-008 (EPA)

Confirmatory: Rapid Radiochemical
Method for Radium-226 in Building
Materials, EPA 402-R14-002 (EPA)

Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Ra-226 half life = 1,600 years)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative: 7500-Ra B CSM1)
Confirmatory: 7500-Ra C CSM)

Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Ra-226 half life = 1,600 years)

Qualitative: No samole
preservation is required if sample
is delivered to the laboratory
within 3 days of collection. If the
sample is to be held for more
than 3 days, add concentrated
HNO3 to achieve a pH < 2.

Confirmatory: Preserve sample
with HNO3 or HCI to pH < 2 (6)

Qualitative: Raoid Radiochemical Method
for Ra-226, EPA 402-R10-001 c (EPA)

Confirmatory: Method for Radium-228
and Radium-226 in Drinking Water (GA
Tech)

Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months
(Ra-226 half life = 1,600 years)

No preservation required

Qualitative: Raoid Method for Radium in
Soil, EPA-600-R-12-635 (EPA)

Confirmatory: AP7 CORISE)

Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Ra-226 half life = 1,600 years)

No preservation required

Qualitative: Rapid Methods for Acid or
Fusion Digestion, EPA 402-R-12-009 and
EPA 402-R-12-008 (EPA)

Confirmatory: Rapid Radiochemical
Method for Radium-226 in Building
Materials, EPA 402-R14-002 (EPA)

Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(Ra-226 half life = 1,600 years)

No preservation required

Qualitative and Confirmatory:
Ra-03-RC (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -18

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Rhenium-188

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Re-188 half life = 16.9 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Re-188 half life = 16.9 hours)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Re-188 half life = 16.9 hours)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Re-188 half life = 16.9 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Re-188 half life = 16.9 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(Re-188 half life = 16.9 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

Rubidium-82

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Rb-82 half life = 75 seconds)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Rb-82 half life = 75 seconds)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Rb-82 half life = 75 seconds)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Rb-82 half life = 75 seconds)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Rb-82 half life = 75 seconds)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(Rb-82 half life = 75 seconds)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -19

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Ruthenium-103

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Ru-103 half life = 39.3 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Ru-103 half life = 39.3 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
7120 (SM)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Ru-103 half life = 39.3 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Ru-103 half life = 39.3 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Ru-103 half life = 39.3 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(Ru-103 half life = 39.3 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

Ruthenium-106

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Ru-106 half life = 374 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Ru-106 half life = 374 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
7120 (SM)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Ru-106 half life = 374 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months
(Ru-106 half life = 374 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Ru-106 half life = 374 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(Ru-106 half life = 374 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -20

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

3ackaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Selenium-75

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Se-75 half life = 120 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Se-75 half life = 120 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
7120 (SM)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Se-75 half life = 120 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Se-75 half life = 120 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Se-75 half life = 120 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(Se-75 half life = 120 days)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -21

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Strontium-89

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Sr-89 half life = 50.53 days)

No preservation required

Qualitative and Confirmatory:

Strontium in Food and Bioenvironmental
Samples, EPA-680/4-75-001 (EPA)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Sr-89 half life = 50.53 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
905.0 (EPA)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months<7)
(Sr-89 half life = 50.53 days)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
905.0 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Sr-89 half life = 50.53 days)

No preservation required

Qualitative: Actinides and

Sr-89/90 in Soil Samples, SRS Manual

L3.23-10054 (DOE SRS)

Confirmatory: Strontium in Food and
Bioenvironmental Samples, EPA-680/4-
75-001 (EPA)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Sr-89 half life = 50.53 days)

No preservation required

Qualitative and Confirmatory:

Strontium in Food and Bioenvironmental
Samples, EPA-680/4-75-001 (EPA)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months<7)
(Sr-89 half life = 50.53 days)

No preservation required

Qualitative: Actinides and
Sr-89/90 in Vegetation, SRS Manual
L3.23-10055 (DOE SRS)

Confirmatory: Strontium in Food and
Bioenvironmental Samples, EPA-680/4-
75-001 (EPA)

SAM Companion - Sample Collection Information Document

B1 -22

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Strontium-90

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Sr-90 half life = 28.8 years)

No preservation required

Qualitative: Rapid Methods for Acid or
Fusion Digestion, EPA 402-R-12-009 and
EPA 402-R-12-008 (EPA)

Confirmatory: Sr-03-RC (HASL-300)

Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months
(Sr-90 half life = 28.8 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
D5811-20 (ASTM)

Drinking Water

2 - 4 L

Samples should be collected in 1-L
plastic containers

Maximum 6 months
(Sr-90 half life = 28.8 years)

Qualitative: No sample
preservation is required if sample
is delivered to the laboratory
within 3 days of sampling
date/time. If the sample is to be
held for more than 3 days, add
concentrated HN03 to achieve a
pH < 2.

Confirmatory: Preserve sample
with HN03 or HCI to pH < 2 (6)

Qualitative: Rapid Radiochemical
Methods for Sr-90, EPA 402-R-10-001d
(EPA)

Confirmatory: 905.0 (EPA)

Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months
(Sr-90 half life = 28.8 years)

No preservation required

Qualitative: Rapid Method for Sodium
Carbonate Fusion of Soil and Soil-
Related Matrices, EPA-600-R-12-640
(EPA)

Confirmatory: Sr-03-RC (HASL-300)

Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(Sr-90 half life = 28.8 years)

No preservation required

Qualitative: Rapid Methods for Acid or
Fusion Digestion, EPA 402-R-12-009 and
EPA 402-R-12-008 (EPA)

Confirmatory: Sr-03-RC (HASL-300)

Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(Sr-90 half life = 28.8 years)

No preservation required

Qualitative: Actinides and Sr-89/90 in
Vegetation, SRS Manual L3.23-10055
(DOE SRS)

Confirmatory: Sr-03-RC (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -23

April 2023

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Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the

container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected131

Sample Container*4*

Holding Time(4)

Sample Preservation*4*

Analytical Method15'

Technetium-99

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

(Tc-99 half life = 211,000 years)

No preservation required

Qualitative and Confirmatory:
AP5 (ORISE)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

(Tc-99 half life = 211,000 years)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
D7168-05 (ASTM)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

(Tc-99 half life = 211,000 years)

Preserve sample with
HNO3 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
Tc-02-RC (HASL-300)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months

(Tc-99 half life = 211,000 years)

No preservation required

Qualitative and Confirmatory:
AP5 (ORISE)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

(Tc-99 half life = 211,000 years)

No preservation required

Qualitative and Confirmatory:
AP5 (ORISE)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months

(Tc-99 half life = 211,000 years)

No preservation required

Qualitative: AP5 (ORISE1)
Confirmatory: Tc-01-RC (HASL-300)

Technetium-99m

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Tc-99m half life = 6 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Tc-99m half life = 6 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'
(Tc-99m half life = 6 hours)

No preservation required

Qualitative and Confirmatory:
901.1 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'
(Tc-99m half life = 6 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'
(Tc-99m half life = 6 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'
(Tc-99m half life = 6 hours)

No preservation required

Qualitative and Confirmatory:
Ga-01-R (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -24

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container'4'

Holding Time'4'

Sample Preservation'4'

Analytical Method15'

Thorium-227
Thorium-228
Thorium-230
Thorium-232

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'

(Th-227 half life = 18.7 days)
(Th-228 half life = 1.9 years)
(Th-230 half life = 75,380 years)
(Th-232 half life = 1.4x 101Gyears)

No preservation required

Qualitative and Confirmatory:
SOP for Actinides in Environmental
Matrices (EPA-NAREL)



Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'

(Th-227 half life = 18.7 days)
(Th-228 half life = 1.9 years)
(Th-230 half life = 75,380 years)
(Th-232 half life = 1.4 x 101Gyears)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative and Confirmatory:
SOP for Actinides in Environmental
Matrices (EPA-NAREL)



Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months'7'

(Th-227 half life = 18.7 days)
(Th-228 half life = 1.9 years)
(Th-230 half life = 75,380 years)
(Th-232 half life = 1.4x 101Gyears)

Preserve sample with
HNO3 or HCI to pH < 2 <6)

Qualitative and Confirmatory:
907.0 (EPA)



Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months'7'

(Th-227 half life = 18.7 days)
(Th-228 half life = 1.9 years)
(Th-230 half life = 75,380 years)
(Th-232 half life = 1.4x 101Gyears)

No preservation required

Qualitative and Confirmatory:
SOP for Actinides in Environmental
Matrices (EPA-NAREL)



Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months'7'

(Th-227 half life = 18.7 days)
(Th-228 half life = 1.9 years)
(Th-230 half life = 75,380 years)
(Th-232 half life = 1.4 x 101G years)

No preservation required

Qualitative and Confirmatory:
SOP for Actinides in Environmental
Matrices (EPA-NAREL)



Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months'7'

(Th-227 half life = 18.7 days)
(Th-228 half life = 1.9 years)
(Th-230 half life = 75,380 years)
(Th-232 half life = 1.4 x 101G years)

No preservation required

Qualitative and Confirmatory:
SOP for Actinides in Environmental
Matrices (EPA-NAREL)

SAM Companion - Sample Collection Information Document

B1 -25

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservation'4'

Analytical Method15'

Tritium
(Hydrogen-3)

Air Filter

For the purposes of this document, this analyte is not considered to be a concern in this sample type

Aqueous & Liquid

2 - 4 L

Borosilicate glass small-mouth bottle

Maximum 6 months
(3H half life = 12.32 years)

No preservation required

Qualitative and Confirmatory:
906.0 (EPA)

Drinking Water

2 - 4 L

Borosilicate glass small-mouth bottle

Maximum 6 months
(3H half life = 12.32 years)

No preservation required

Qualitative and Confirmatory:
906.0 (EPA)

Soil & Sediment

100 g

Borosilicate glass wide-mouth jar

Maximum 6 months
(3H half life = 12.32 years)

No preservation required. Tritium
will volatilize with heat; store
samples at room temperature if
stored before shipping.

Qualitative and Confirmatory:
AP2 (ORISE)

Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months
(3H half life = 12.32 years)

No preservation required. Tritium
will volatilize with heat; do not dry
sample before analysis.

Qualitative and Confirmatory:
AP2 (ORISE)

Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months
(3H half life = 12.32 years)

No preservation required. Tritium
will volatilize with heat; do not dry
sample prior to analysis.

Qualitative and Confirmatory:
AP2 (ORISE)

SAM Companion - Sample Collection Information Document

B1 -26

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack samples
outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container14*

Holding Time(4)

Sample Preservation'4'

Analytical Method15'

Uranium-234
Uranium-235
Uranium-238

Air Filter

1 filter

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

•	(U-234 half life = 244,000 years)

•	(U-235 half life = 700 x 106 years)

•	(U-238 half life = 4.47 x 109 years)

No preservation required

Qualitative: Raoid Methods for Acid or
Fusion Digestion, EPA 402-R-12-009 and
EPA 402-R-12-008 (EPA)

Confirmatory: SOP for Actinides in
Environmental Matrices (EPA-NAREL)

Aqueous & Liquid

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

•	(U-234 half life = 244,000 years)

•	(U-235 half life = 700 x 106 years)

•	(U-238 half life = 4.47 x 109 years)

Preserve sample with
HN03 or HCI to pH < 2 (6)

Qualitative: 7500-U B fSM)
Confirmatory: 7500-U C (SM)

Drinking Water

2 - 4 L

Plastic (polypropylene or
polyethylene) or borosilicate glass
small-mouth bottle

Maximum 6 months

•	(U-234 half life = 244,000 years)

•	(U-235 half life = 700 x 106 years)

•	(U-238 half life = 4.47 x 109 years)

Qualitative: No samole
preservation is required if sample
is delivered to the laboratory
within 3 days of collection. If the
sample is to be held for more
than 3 days, add concentrated
HNO3 to achieve a pH < 2.

Confirmatory: Preserve sample
with HN03 or HCI to pH < 2 (6)

Qualitative: Raoid Radiochemical Method
for U, EPA 402-R-10-001 e (EPA)

Confirmatory: D3972-09 (2015) fASTM)

Soil & Sediment

100 g

Plastic (polypropylene or
polyethylene) large-mouth bottle, jar
or plastic bag. Borosilicate glass
wide-mouth jar may also be used.

Maximum 6 months

•	(U-234 half life = 244,000 years)

•	(U-235 half life = 700 x 106 years)

•	(U-238 half life = 4.47 x 109 years)

No preservation required

Qualitative: Rapid Method for Fusion of
Soil and Soil-Related Matrices, EPA 600-
R-12-636, EPA 600-R-12-637 and EPA
600-R-12-638 (EPA)

Confirmatory: SOP for Actinides in
Environmental Matrices (EPA-NAREL)

Surface Wipes

1 wipe

Glassine envelope, polyacrylic
plastic Petri dish, or polyethylene
plastic bag

Maximum 6 months

•	(U-234 half life = 244,000 years)

•	(U-235 half life = 700 x 106 years)

•	(U-238 half life = 4.47 x 109 years)

No preservation required

Qualitative: Rapid Methods for Acid or
Fusion Digestion, EPA 402-R-12-009 and
EPA 402-R-12-008 (EPA)

Confirmatory: SOP for Actinides in
Environmental Matrices (EPA-NAREL)

Vegetation

1-gallon tightly packed
zipper-locked bag or
plastic container

Plastic (polypropylene or
polyethylene) bag with zipper lock
and durability to resist punctures OR
wide-mouth plastic container

Maximum 6 months

•	(U-234 half life = 244,000 years)

•	(U-235 half life = 700 x 106 years)

•	(U-238 half life = 4.47 x 109 years)

No preservation required

Qualitative: Actinides and
Sr-89/90 in Vegetation, SRS Manual
L3.23-10055 (DOE SRS)

Confirmatory: U-02-RC (HASL-300)

SAM Companion - Sample Collection Information Document

B1 -27

April 2023

-------
Sample Collection Information Document - Attachment B1
April 2023

Footnotes:

(1) Sample transport containers are packed outside the contaminated area. Samples must be packed in a manner that protects the integrity of the containers. Samples should be surrounded by shock absorbing and liquid
absorbent packing materials (49 CFR 173.24 and 173.453). Check the radiation-specific activity from the packaged samples (49 CFR 173.403) and record results on a field sample tracking form (see EPA's Sample
Collection Procedures for Radiochemical Analytes in Environmental Matrices [https://cfpub.epa.gov/si/si_public_record_report.cfm?dirEntryld=350579&Lab=CESER])

(2) Although the package required for transporting radioactive material is based on the activity INSIDE the package, the label required on the transport package is based on the radiation hazard OUTSIDE the package.
Radioactive material is the only hazardous material that has three possible labels, depending on the relative radiation levels external to the package. Labels for radioactive material also require a Transportation Index (Tl)
number which indicates the highest radiation level measured at 1 meter from the surface of the package. The three possible labels are commonly called White 1, Yellow 2, and Yellow 3, referring to the color of the label
and the number prominently displayed. The specific label required (White 1, Yellow 2, or Yellow 3) is based on the limits specified in 49 CFR 172.403.

(3) Sample sizes are provided for guidance, and may vary depending on the specific contamination incident, data quality objectives and requirements, and laboratory radiochemistry license considerations. With the
exception of total gamma, the sample sizes listed are based on the amount needed to support analyses addressing all the radioisotopes listed in this attachment; the sample size listed for total gamma is based on the
amount needed to determine only total gamma radiation.

(4) Sample container, holding times, preservation, and sample preparation information has been derived from requirements stated in the analytical methods, documents cited under "Analytical Method" or in Section 7.0 of
this document, or best professional judgment.

(5) Methods listed in this column can be located using U.S. Environmental Protection Agency's Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2022 (https://www.epa.gov/esam/
selected-analytical-methods-environmental-remediation-and-recovery-sam). The SAM document is intended to be used concurrently with this Sample Collection Information Document. Full citations for references not cited
and/or accessed through the SAM website are provided in Section 7.0 of this document.

(6) If dissolved components are to be determined, samples requiring preservation must be filtered prior to preservation. Drinking water samples can be preserved in the laboratory within 5 days of collection, if sample
collectors are not able to preserve the samples in the field.

(7) Holding times of short-lived radionuclides may need to be adjusted to be significantly less than 6 months on a case-by-case basis, depending on the half-life of the isotope, its detectability, and its initial concentration.

(8) This procedure should be used only for filters specifically designed for iodine.

SAM Companion - Sample Collection Information Document

B1 -28

April 2023

-------
Attachment B2

Sample Collection Information for the
Outdoor Building and Infrastructure
Materials, Radiochemical Analytes and
Methods Listed in SAM 2022

[Note: The acronyms and abbreviations used in this
attachment are listed and defined in the beginning of the

report.]

-------
Sample Collection Information Document - Attachment B2
April 2023

Attachment B2: Sample Collection Information for the Outdoor Building and Infrastructure Materials, Radiochemical Analytes and Methods
Listed in SAM 2022

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack
samples outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time(4)

Sample Preservative*4*

Sample Preparation Methods*5*

Analytical Methods*5*

Americium-241

Asphalt shingles

250 grams

Chips, rubble, particulates:
250-mL or larger plastic jars
or sample bottles

Cores, cuttings, bulk
materials: Large zip-locking
or plastic bag with ties,
and/or box (steel, wood or
fiberboard)

Maximum 6 months

(Am-241 half life =
432 years)

No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Asphalt Matrices Prior to
Americium, Plutonium, Strontium,
Radium, and Uranium Analyses, EPA
402-R16-001 (EPANAREL)

~ Rapid Radiochemical Method
for Americium-241 in Building
Materials for Environmental
Remediation Following
Radiological Incidents, EPA 402-
R14-007 (EPA NAREL)



Asphalt paving
material

25 grams



No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Asphalt Roofing Material
Matrices Prior to Americium, Plutonium,
Strontium, Radium, and Uranium
Analyses, EPA 402-R16-003 (EPA
NAREL)



Concrete

25 grams





No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Concrete and Brick Matrices





Brick

25 grams





No preservation required

Prior to Americium, Plutonium, Strontium,
Radium, and Uranium Analyses for





Granite (6)

25 grams





No preservation required

Environmental Remediation Following
Radiological Incidents, EPA 402-R-14-
004 (EPA NAREL)





Stucco |6'

25 grams





No preservation required





Limestone

25 grams





No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Limestone Matrices Prior to
Americium, Plutonium, Strontium,
Radium, and Uranium Analyses for
Environmental Remediation Following
Radiological Incidents, EPA 402-R-18-
002 (EPA NAREL)



Sample Collection Information Document

B2-1

April 2023

-------
Sample Collection Information Document - Attachment B2
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack
samples outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container'4'

Holding Time'4'

Sample Preservative^

Sample Preparation Methods*5*

Analytical Methods*5*

Plutonium-238
Plutonium-239

Asphalt shingles

250 grams

Chips, rubble, particulates:
250-mL or larger plastic jars
or sample bottles

Cores, cuttings, bulk
materials: Large zip-locking
or plastic bag with ties,
and/or box (steel, wood or
fiberboard)

Maximum 6 months

(Pu-238 half life =
87.7 years)

(Pu-239 half life =
24,100 years)

No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Asphalt Matrices Prior to
Americium, Plutonium, Strontium,
Radium, and Uranium Analyses, EPA
402-R16-001 (EPANAREL)

~ Rapid Radiochemical Method
for Plutonium-238 and
Plutonium-239/240 in Building
Materials for Environmental
Remediation Following
Radiological Incidents, EPA 402
R14-006 (EPA NAREL)



Asphalt paving
material

25 grams

No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Asphalt Roofing Material
Matrices Prior to Americium, Plutonium,
Strontium, Radium, and Uranium
Analyses, EPA 402-R16-003 (EPA
NAREL)



Concrete

25 grams





No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Concrete and Brick Matrices





Brick

25 grams





No preservation required

Prior to Americium, Plutonium, Strontium,
Radium, and Uranium Analyses for





Granite 161

25 grams





No preservation required

Environmental Remediation Following
Radiological Incidents, EPA402-R-14-





Stucco 161

25 grams





No preservation required

004 (EPA NAREL)





Limestone

25 grams





No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Limestone Matrices Prior to
Americium, Plutonium, Strontium,
Radium, and Uranium Analyses for
Environmental Remediation Following
Radiological Incidents, EPA 402-R-18-
002 (EPA NAREL)



Sample Collection Information Document

B2-2

April 2023

-------
Sample Collection Information Document - Attachment B2
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack
samples outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container(4>

Holding Time'4'

Sample Preservative*4*

Sample Preparation Methods*5*

Analytical Methods'5*

Radium-226

Asphalt shingles

250 grams

Chips, rubble, particulates:
250-mL or larger plastic jars
or sample bottles

Cores, cuttings, bulk
materials: Large zip-locking

Maximum 6 months

(Ra-226 half life =
1,600 years)

No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Asphalt Matrices Prior to
Americium, Plutonium, Strontium,
Radium, and Uranium Analyses, EPA
402-R16-001 (EPANAREL)

~ Rapid Radiochemical Method
for Radium-226 in Building
Materials for Environmental
Remediation Following
Radiological Incidents, EPA 402
R14-002 (EPA NAREL)



Asphalt paving
material

25 grams

or plastic bag with ties,
and/or box (steel, wood or
fiberboard)



No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Asphalt Roofing Material
Matrices Prior to Americium, Plutonium,
Strontium, Radium, and Uranium
Analyses, EPA 402-R16-003 (EPA
NAREL)





Concrete

25 grams





No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Concrete and Brick Matrices
Prior to Americium, Plutonium, Strontium,
Radium, and Uranium Analyses for
Environmental Remediation Following

~ Rapid Radiochemical Method
for Radium-226 in Building
Materials for Environmental
Remediation Following
Radiological Incidents, EPA 402



Brick

25 grams





No preservation required



Granite <6)

25 grams





No preservation required

Radiological Incidents, EPA 402-R-14-
004 (EPA NAREL)

R14-002 (EPA NAREL)



Stucco <6)

25 grams





No preservation required







Limestone

25 grams





No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Limestone Matrices Prior to
Americium, Plutonium, Strontium,
Radium, and Uranium Analyses for
Environmental Remediation Following
Radiological Incidents, EPA 402-R-18-
002 (EPA NAREL)



Sample Collection Information Document

B2-3

April 2023

-------
Sample Collection Information Document - Attachment B2
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack
samples outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container*4*

Holding Time141

Sample Preservative'4*

Sample Preparation Methods*5*

Analytical Methods'5*

Strontium-90

Asphalt shingles

250 grams

Chips, rubble, particulates:
250-mL or larger plastic jars
or sample bottles

Cores, cuttings, bulk
materials: Large zip-locking
or plastic bag with ties,
and/or box (steel, wood or
fiberboard)

Maximum 6 months

(Sr-90 half life = 28.8
years)

No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Asphalt Matrices Prior to
Americium, Plutonium, Strontium,
Radium, and Uranium Analyses, EPA
402-R16-001 (EPANAREL)

~ Rapid Radiochemical Method
for Total Radiostrontium (Sr-90)
in Building Materials for
Environmental Remediation
Following Radiological
Incidents, EPA 402-R14-001
(EPA NAREL)



Asphalt paving
material

25 grams



No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Asphalt Roofing Material
Matrices Prior to Americium, Plutonium,
Strontium, Radium, and Uranium
Analyses, EPA 402-R16-003 (EPA
NAREL)



Concrete

25 grams





No preservation required

• Rapid Method for Sodium Hydroxide
Fusion of Concrete and Brick Matrices





Brick

25 grams





No preservation required

Prior to Americium, Plutonium, Strontium,
Radium, and Uranium Analyses for
Environmental Remediation Following
Radiological Incidents, EPA402-R-14-
004 (EPA NAREL)





Granite 161

25 grams





No preservation required





Stucco 161

25 grams





No preservation required





Limestone

25 grams





No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Limestone Matrices Prior to
Americium, Plutonium, Strontium,
Radium, and Uranium Analyses for
Environmental Remediation Following
Radiological Incidents, EPA 402-R-18-
002 (EPA NAREL)



Sample Collection Information Document

B2-4

April 2023

-------
Sample Collection Information Document - Attachment B2
April 2023

Packaging Requirements: Wipe outside of each container clean using a damp, then dry cloth. Seal the container with non-reactive tape or film. Determine the radiation-specific activity of the sample(s) and pack
samples outside the contaminated area as described in Footnote (1).

Shipping Label: Shipping labels per requirements of 49 CFR 173. Determine the radiation levels on the surface and at 1 meter from the surface of the package, and label. Refer to information in Footnote (2).

Analyte

Sample Type

Sample Size
Collected'3'

Sample Container(4>

Holding Time'4'

Sample Preservative^

Sample Preparation Methods*5*

Analytical Methods*5*

Uranium-234
Uranium-235
Uranium-238

Asphalt shingles

250 grams

Chips, rubble, particulates:
250-mL or larger plastic jars
or sample bottles

Cores, cuttings, bulk
materials: Large zip-locking
or plastic bag with ties,
and/or box (steel, wood or
fiberboard)

Maximum 6 months

(U-234 half life =
244,000 years)

(U-235 half life =
700 million years)

(U-238 half life =
4.47 billion years)

No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Asphalt Matrices Prior to
Americium, Plutonium, Strontium,
Radium, and Uranium Analyses, EPA
402-R16-001 (EPANAREL)

~ Rapid Radiochemical Method
for Isotopic Uranium in Building
Materials for Environmental
Remediation Following
Radiological Incidents, EPA 402
R14-005 (EPA NAREL)



Asphalt paving
material

25 grams

No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Asphalt Roofing Material
Matrices Prior to Americium, Plutonium,
Strontium, Radium, and Uranium
Analyses, EPA 402-R16-003 (EPA
NAREL)



Concrete

25 grams





No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Concrete and Brick Matrices





Brick

25 grams





No preservation required

Prior to Americium, Plutonium, Strontium,
Radium, and Uranium Analyses for





Granite <6)

25 grams





No preservation required

Environmental Remediation Following
Radiological Incidents, EPA 402-R-14-





Stucco <6)

25 grams





No preservation required

004 (EPA NAREL)





Limestone

25 grams





No preservation required

~ Rapid Method for Sodium Hydroxide
Fusion of Limestone Matrices Prior to
Americium, Plutonium, Strontium,
Radium, and Uranium Analyses for
Environmental Remediation Following
Radiological Incidents, EPA 402-R-18-
002 (EPA NAREL)



Sample Collection Information Document

B2-5

April 2023

-------
Sample Collection Information Document - Attachment B2
April 2023

Footnotes:

(1)	Sample transport containers are packed outside the contaminated area. Samples must be packed in a manner that protects the integrity of the sample containers. Samples should be surrounded by shock absorbing
and liquid absorbent packing materials (49 CFR 173.24 and 173.453). Check the radiation-specific activity from the packaged samples (49 CFR 173.403) and record results on a field sample tracking form (see EPA's
Sample Collection Procedures for Radiochemical Analytes in Outdoor Building and Infrastructure Materials rhttPs://cfpub.epa.aov/si/si public record report.cfm?Lab=NHSRC&dirEntrvld=335065T).

(2)	Although the package required for transporting radioactive material is based on the activity INSIDE the package, the label required on the transport package is based on the radiation hazard OUTSIDE the package.
Radioactive material is the only hazardous material that has three possible labels, depending on the relative radiation levels external to the package. Labels for radioactive material also require a Transportation Index (Tl)
number which indicates the highest radiation level measured at 1 meter from the surface of the package. The three possible labels are commonly called White 1, Yellow 2, and Yellow 3, referring to the color of the label
and the number prominently displayed. The specific label required (White 1, Yellow 2, or Yellow 3) is based on the limits specified in 49 CFR 172.403.

(3)	Sample sizes are provided for guidance, and may vary depending on the specific contamination incident, data quality objectives and requirements, and laboratory radiochemistry license considerations. The sample
sizes listed are based on the amount needed to support analyses addressing all the radioisotopes listed in this attachment.

(4)	Sample container, holding times, preservation, and sample preparation information has been derived from requirements stated in the analytical methods, documents cited under "Analytical Method" or in Section 7.0
of this document, or best professional judgment.

(5)	Methods listed in this column can be located using U.S. Environmental Protection Agency's "Selected Analytical Methods for Environmental Remediation and Recovery (SAM) 2022"

(https://www.epa.aov/esam/selected-analvtical-methods-environmental-remediation-and-recoverv-sam'). The SAM document is intended to be used concurrently with this Sample Collection Information Document. Full
citations for references not cited and/or accessed through the SAM website are provided in Section 7.0 of this document.

(6)	EPA methods are currently under development for granite and stucco. When updates become available, information will be provided on the SAM website at https://www.epa.gov/esam/selected-analytical-methods-
environmental-remediation-and-recoverv-sam.

Sample Collection Information Document

B2-6

April 2023

-------
Attachment C

Sample Collection Information for the
Pathogen Analytes and Methods Listed in

SAM 2022

[Note: The acronyms and abbreviations used in this
attachment are listed and defined in the beginning of the

report.]

-------
Sample Collection Information Document - Attachment C

April 2023

Attachment C: Sample Collection Information for the Pathogen Analytes and Methods Listed in SAM 2022

This attachment is intended to be used with SAM 2022 and contains summary information for collection of samples to be analyzed for the selected pathogens using the methods and procedures listed in SAM. Step-by-step instructions on collecting samples for SAM
oathoaens can be found on the ESAM website at: httDs://www.eDa.aov/esam/samDle-collection-Drocedures-and-strateaies.

Packaging and Shipping Requirements:

Sampling personnel should refer to the below websites for information regarding infectious substance categories (i.e., Category A and B), packaging and shipping. It is the sample originator's responsibility to maintain training and ensure adherence to the most current
regulations.

• International Air Transoort Association CIATA") ChttDs://www.iata.ora/en/Dublications/dar/")

• U.S. Deoartment of Transoortation CUSDOT") fhttos://aov.ecfr.io/cai-bin/text-idx?SID=51627edf054ac9506f03a5729aa8cbb9&mc=true&tol=/ecfrbrowse/Title49/49tab O2.toh

• American Societv for Microbioloav ChttD://www.asm.ora/")

• American Bioloaical Safetv Association fhtto://www.absa.ora/")

• World Health Oraanization Guidance on Reaulations for the Transoort of Infectious Substances fhttos://aoDs.who.int/iris/handle/10665/339825")

Note: The level of packaging required for samples is dependent on whether the samples are considered a dangerous goods shipment or not. In general, environmental samples which are not considered to pose a significant risk of infection are not subject to the IATA or
USDOT shipping requirements and are considered "exempt1.

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation45

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Bacteria7

Bacillus anthracis

[Anthrax]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA Bacillus anthracis (BA)
Protocol (EPA/600/R-17/213)
[Tier I]

Culture/Real-time PCR/RV-PCR:
• EPA BA Protocol
(EPA/600/R-17/213) [Tier I]

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wioes: 144-inch2
Filter Cassettes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch *
6 inch sealable plastic bags for containment of
the filter cassette

Soil

50 g - 500 g

50 - 500-mL sterile container

Silvestri et al. 2016. J. of Microbiol.
Methods. 130: 6-13 [Tier II]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

EPA BA Protocol
(EPA/600/R-17/213) [Tier I]

SAM Companion - Sample Collection Information Document

C-1

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Brucella spp.

(6. abortus,
B. melitensis,

B. suis)
[Brucellosis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA Yersinia pestis (YP) Protocol
(EPA/600/R-16/109) [Tier III]

Culture:

•ASM Sentinel Level Clinical
Microbiology Laboratory Guidelines for
Suspected Agents of Bioterrorism and
Emerging Infectious Diseases: Brucella
species [Tier I]

Real-Time PCR;

• Hinic et al. 2008. J. Microbiol. Methods.
75(2): 375-378 [Tier II]

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Soonae-Sticks: 100-inch2
Wioes: 144-inch2
Filter Cassettes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1682
(EPA-821-R-06-14) [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

Burkholderia mallei

[Glanders] and
Burkholderia
pseudomallei
[Melioidosis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

Culture:

•ASM Sentinel Level Clinical
Microbiology Laboratory Guidelines for
Suspected Agents of Bioterrorism and
Emerging Infectious Diseases:
Burkholderia mallei and S.
pseudomallei [Tier I]

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wioes: 144-inch2
Filter Cassettes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette



•	Tomaso et al. 2006. Clin. Chem. 52(2):
307-310

and

•	Novak et al. 2006. J. Clin. Microbiol.
44(1): 85-90 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Hall etal. 2019. PLoS Negl. Trop.
Dis. 13(9):e0007727 [Tier II]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

SAM Companion - Sample Collection Information Document

C - 2

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Campylobacter jejuni

[Campylobacteriosis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Soonae-Sticks: 100-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: 144-inch2
Filter Cassettes: 144-inch2

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

•	ISO 17995 [Tier I]

Real-Time PCR:

•	Cunningham et al. 2010. J. Clin.
Microbiol. 48(8): 2929-2933 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Hiett. 2017. Methods Mol. Biol.
1512:1-8 [Tier II]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

Chlamydophila
psittaci

("formerly known as
Chlamydia psittaci)
[Psittacosis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

Tissue Culture/PCR:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wipes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: 144-inch2

sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

• Madico et al. 2000. J. Clin. Microbiol.
38(3): 1085-1093 [Tier II]

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1682
(EPA-821-R-06-14) [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

SAM Companion - Sample Collection Information Document

C - 3

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Coxiella burnetii

[Q-fever]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA BA Protocol
(EPA/600/R-17/213) [Tier III]

Tissue Culture;

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Soonae-Sticks: 100-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

•	Hodges et al. 2010. J. Microbiol.
Methods. 81(2): 141-146 [Tier III]
or

•	Rose et al. 2011. Appl. Environ.
Microbiol. 77(23): 8355-8359 [Tier
III]

or

•	EPA BA Protocol
(EPA/600/R-17/213) [Tier III]

Wioes: 144-inch2
Filter Cassettes: 144-inch2

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

• Raoultetal. 1991. Antimicrob. Agents
Chemother. 35(10): 2070-2077 [Tier II]

Real-Time PCR:



• Panning etal. 2008. BMC Microbiol.
8:77 [Tier II]

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1682
(EPA-821-R-06-14) [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 mL- 100 L

100-mL or larger sterile container

•	EPA and CDC Joint Collection
Protocol (Ultrafiltration [UF])
(EPA 600/R-21/280)

and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

Escherichia coii

0157:H7

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch * 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: 144-inch2
Filter Cassettes: 144-inch2

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

• EPA Eschericia coli 0157:H7 (EC)
Protocol (EPA/600/R-10/056) [Tier I]

Real-Time PCR:



• Sen et al. 2011. Environ. Sci. Technol.
45(7): 2250-2256 [Tier II]

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1680
(EPA-821-R-14-009) [Tier I]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 mL- 100 L

100-mL or larger sterile container

EPA EC Protocol
(EPA/600/R-10/056) [Tier I]

SAM Companion - Sample Collection Information Document

C - 4

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Francisella tularensis

[Tularemia]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at £ 10 °C. Do
not allow samples to freeze.

EPA Francisella tularensis (FT)
Protocol (EPA/600/R-19/110) [Tier I]

Culture/Real-Time PCR/RV-PCR;
• EPA FT Protocol (EPA/600/R-19/110)
[Tier I]

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wioes: 144-inch2
Filter Cassettes: 144-inch

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1682
(EPA-821 -R-06-14) [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

EPA FT Protocol
(EPA/600/R-19/110) [Tier I]

Legionella
pneumophila

[Legionellosis ]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

US DHHS. 2005. Procedures for the
Recovery of Legionella from the
Environment [Tier I]

Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Kozaketal. 2013. Identification of
Legionella in the Environment.
Methods Mol. Biol. 954: 3-25 [Tier I]

Wioes: 144-inch2
Filter Cassettes: 144-inch2

• Kozak et al. 2013. Identification of
Legionella in the Environment. Methods
Mol. Biol. 954: 3-25 [Tier I]



• ISO Method ISO/TS 12869:2019 [Tier I]

Soil

50 g - 500 g

50 - 500-mL sterile container

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

SAM Companion - Sample Collection Information Document

C - 5

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Leptospira
interrogans

[Leptospirosis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Water samples can be kept at
ambient temperature for analysis
within 72 hours of collection.

All other samples should be
analyzed as soon as possible.
Holding time of 24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: 144-inch2
Filter Cassettes: 144-inch

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

• Standard Method 9260 I; Leptospira
[Tier I]

Real-Time PCR:



• Palaniappan et al. 2005. Mol. Cell
Probes. 19(2): 111-117 [Tier II]

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1682
(EPA-821-R-06-14) [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 mL- 100 L

100-mL or larger sterile container

Standard Method 9260 I; Leptospira
[Tier I]

Listeria
monocytogenes

[Listeriosis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

After sampling, swabs and
sponge-sticks should be
maintained in D/E
neutralizing broth prior to
analyses.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

Culture:

•	Hitchins et al. 2017. Bacteriological
Analytical Manual Online [Tier I]

Real-Time PCR:

•	USDA, FSIS. 2021. Microbiology
Laboratory Guidebook MLG 8.13 [Tier I]

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wioes: 144-inch2
Filter Cassettes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Soil

50 g - 500 g

50 - 500-mL sterile container

Iwu and Okoh. 2020. PLoS ONE.
15(2): e0228956 [Tier II]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 mL- 100 L

100-mL or larger sterile container

SAM Companion - Sample Collection Information Document

C - 6

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Non-typhoidal
Salmonella

(Not applicable to
S. Typhi)
[Salmonellosis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at £ 10 °C. Do
not allow samples to freeze.

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

Culture:

•	EPA Method 1682
(EPA-821-R-06-14) [Tier I]
or

•	EPA Method 1200
(EPA 817-R-12-004 [Tier I]

Real-Time PCR:

•	Jyoti et al. 2011. Environ. Sci. Technol.
45(20): 8996-9002 [Tier II]

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Soonae-Sticks: 100-inch2
Wioes: 144-inch2
Filter Cassettes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1682
(EPA-821-R-06-14) [Tier I]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 mL- 100 L

100-mL or larger sterile container

EPA Method 1200
(EPA 817-R-12-004) [Tier I]

Salmonella Typhi

[Typhoid fever]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch * 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wioes: 144-inch2
Filter Cassettes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wipes: Sterile 50-mL centrifuqe tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

• EPA Salmonella Typhi (ST) Protocol
(EPA 600/R-10/133) [Tier I]



• CDC Laboratory Assay [Tier I]

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1682
(EPA-821-R-06-14) [Tier I]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 mL- 100 L

100-mL or larger sterile container

EPA ST Protocol

(EPA 600/R-10/133) [Tier I]

SAM Companion - Sample Collection Information Document

C - 7

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Shigella spp.

[Shigellosis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at £ 10 °C. Do
not allow samples to freeze.

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]



Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Soonae-Sticks: 100-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: 144-inch2
Filter Cassettes: 144-inch2

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

• Standard Method 9260 E: Shigella
[Tier I]

Real-Time PCR:



• Cunningham etal. 2010. J. Clin.
Microbiol. 48(8): 2929-2933 [Tier II]

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1682
(EPA-821 -R-06-14) [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

Standard Method 9260 E: Shigella
[Tier I]

Staphylococcus
aureus

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Sponae-Sticks: 100-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: 144-inch2

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

• Standard Method 9213 B:
Staphylococcus aureus [Tier I]

Real-Time PCR:



• Chiang et al. 2007. J. Food Prot.
70(12): 2855-2859 [Tier II]

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1682
(EPA-821-R-06-14) [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

Li et al. 2015. Environ. Sci.
Technol. 49: 14249-14256 [Tier II]

SAM Companion - Sample Collection Information Document

C - 8

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Vibrio cholerae

[Cholera]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Samples to be
analyzed for Vibrio cholerae
should be kept at room
temperature and must not be
cooled.

Samples to be analyzed for
Vibrio cholerae should be
kept at room temperature
and must not be cooled.

EPA YP Protocol
(EPA/600/R-16/109) [Tier III]

Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: 144-inch2

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

• EPA Vibrio cholerae (VC) Protocol
(EPA 600/R-10/139) [Tier I]

Real-Time PCR:



• Blackstone etal. 2007. J. Microbiol.
Methods. 68(2): 254-259 [Tier II]

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1682
(EPA-821 -R-06-14) [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

EPA VC Protocol (EPA 600/R-
10/139) [Tier I]

Yersinia pestis

[Plague]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 48 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA YP Protocol
(EPA/600/R-16/109) [Tier I]

Culture/Real-Time PCR/RV-PCR:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wipes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: 144-inch2

sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

• EPA YP Protocol (EPA/600/R-16/109)
[Tier I]

Soil

30 g minimum

50 - 500-mL sterile container

EPA Method 1682
(EPA-821-R-06-14) [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

EPA YP Protocol
(EPA/600/R-16/109) [Tier I]

SAM Companion - Sample Collection Information Document

C - 9

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4,5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Viruses9

Adenoviruses:

Enteric and
non-enteric (A-F)

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch * 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 72 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

Raynor et al. 2021. PLoS ONE.
16(1): e0244977 [Tier III]

Tissue Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Park etal. 2015. Appl. Environ.
Microbiol. 81(17): 5987-5992 [Tier
III]

Wioes: 144-inch2
Filter Cassettes: 144-inch2

•	Boczek et al. 2016. J. Microbiol.
Methods. 122: 43-49 [Tier II]

or

•	Green and Loewenstein. 2005. Curr.
Protoc. Microbiol. 14C.1.1-14C.1.19
[Tier II]

Real-Time PCR:

•	Jothikumar etal. 2005. Appl. Environ.
Microbiol. 71(6): 3131-3136 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Staggemeier et al. 2015. J Virol.
Methods. 213: 65-67 [Tier II]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA Method 1642

(EPA 820-R-18-001) [Tier III]
or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)
and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

Astroviruses

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 72 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

Raynor et al. 2021. PLoS ONE.
16(1): e0244977 [Tier III]

Intearated Cell Culture /

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wioes: 144-inch2
Filter Cassettes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Park et al. 2015. Appl. Environ.
Microbiol. 81(17): 5987-5992 [Tier
III]



• Grimm et al. 2004. Can. J. Microbiol.
50(4): 269-278 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Staggemeier et al. 2015. J Virol.
Methods. 213: 65-67 [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA Method 1642

(EPA 820-R-18-001) [Tier III]
or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)
and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

SAM Companion - Sample Collection Information Document

C-10

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Caliciviruses:
Noroviruses

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 72 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at £ 10 °C. Do
not allow samples to freeze.

Raynor et al. 2021. PLoS ONE.
16(1): e0244977 [Tier III]

Real-time Reverse Transcriotion-PCR:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Soonae-Sticks: 100-inch2
Wioes: 144-inch2
Filter Cassettes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Park et al. 2015. Appl. Environ.
Microbiol. 81(17): 5987-5992 [Tier
III]



• EPA Method 1615
(EPA/600/R-10/181) [Tier I]

Soil

50 g - 500 g

50 - 500-mL sterile container

Staggemeier et al. 2015. J Virol.
Methods. 213: 65-67 [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA Method 1642

(EPA 820-R-18-001) [Tier III]
or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)
and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

Caliciviruses:
Sapovirus

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 72 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

Raynor etal. 2021. PLoS ONE.
16(1): e0244977 [Tier III]

Tissue Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wipes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Park et al. 2015. Appl. Environ.
Microbiol. 81(17): 5987-5992 [Tier
III]

Filter Cassettes: 144-inch2

sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

•	Parwani et al. 1991. Arch. Virol.
120(1-2): 115-122 [Tier II]

Real-time Reverse Transcriotion-PCR:

•	Oka et al. 2006. J. Med. Virol. 78(10):
1347-1353 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Staggemeier et al. 2015. J Virol.
Methods. 213: 65-67 [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA Method 1642

(EPA 820-R-18-001) [Tier III]
or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)
and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

SAM Companion - Sample Collection Information Document

C-11

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Coronaviruses:
SARS-associated
human coronavirus
(SARS-CoV-2, SARS-
CoV, and MERS-CoV)

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 72 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at £ 10 °C. Do
not allow samples to freeze.

Raynor et al. 2021. PLoS ONE.
16(1): e0244977 [Tier III]

Tissue Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Soonae-Sticks: 100-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Shah et al. 2021. J. Virol. Methods.
297. 114251 [Tier II]

Wioes: 144-inch2

• Pagat et al. 2007. Applied Biosafety
12(2): 100-108 [Tier II]

Real-time Reverse Transcriotion-PCR:



•	McMinn et al. 2021 Sci. Total Environ.
774: 145727 [Tier II]

Raoid Viabilitv-Reverse Transcriotion-
PCR:

•	Shah et al. 2021. J. Virol. Methods.
297. 114251 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Staggemeieretal. 2015. J Virol.
Methods. 213: 65-67 [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 mL- 100 L

100-mL or larger sterile container

•	EPA Method 1642

(EPA 820-R-18-001) [Tier III]
or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)
and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

Hepatitis E virus
(HE V)

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch * 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 72 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

Raynor et al. 2021. PLoS ONE.
16(1): e0244977 [Tier III]

Tissue Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wipes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Park et al. 2015. Appl. Environ.
Microbiol. 81(17): 5987-5992 [Tier
III]

Filter Cassettes: 144-inch2

sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

•	Zaki et al. 2009. Pathog. Dis. 56: 73-79
[Tier II]

Real-time Reverse Transcriotion-PCR:

•	Jothikumar et al. 2006. J. Virol.
Methods. 131(1): 65-71 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Staggemeier et al. 2015. J Virol.
Methods. 213: 65-67 [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 mL- 100 L

100-mL or larger sterile container

•	EPA Method 1642

(EPA 820-R-18-001) [Tier III]
or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)
and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

SAM Companion - Sample Collection Information Document

C-12

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Influenza H5N1 virus

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 72 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at £ 10 °C. Do
not allow samples to freeze.

Raynor et al. 2021. PLoS ONE.
16(1): e0244977 [Tier II]

Tissue Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Soonae-Sticks: 100-inch2
Wioes: 144-inch2
Filter Cassettes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wipes: Sterile 50-mL centrifuqe tube

Park et al. 2015. Appl. Environ.
Microbiol. 81(17): 5987-5992 [Tier
III]

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

•	Krauss et al. 2012. Influeza Virus
Isolation. Methods Mol. Biol. 865: 11-24
[Tier II]

Real-Time Reverse Transcriotion-PCR:

•	Ng etal. 2005. Emerg. Infect. Dis.
11(8): 1303-1305 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Staggemeier et al. 2015. J Virol.
Methods. 213: 65-67. [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)
and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

Picornaviruses:
Enteroviruses

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 72 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

Raynor etal. 2021. PLoS ONE.
16(1): e0244977 [Tier III]

Tissue Culture/Reverse Transcriotion-

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wioes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Park et al. 2015. Appl. Environ.
Microbiol. 81(17): 5987-5992 [Tier
III]



PCR:

• EPA Method 1615
(EPA/600/R-10/181) [Tier I]

Soil

50 g - 500 g

50 - 500-mL sterile container

Staggemeier et al. 2015. J Virol.
Methods. 213: 65-67 [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA Method 1642

(EPA 820-R-18-001) [Tier III]
or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)
and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

SAM Companion - Sample Collection Information Document

C-13

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Picornaviruses:
Hepatitis A virus
(HAV)

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 72 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

Raynoretal. 2021. PLoS ONE.
16(1): e0244977 [Tier III]

Intearated Cell Culture/Real-Time

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Soonae-Sticks: 100-inch2
Wioes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Parketal. 2015. Appl. Environ.
Microbiol. 81(17): 5987-5992 [Tier
III]



Reverse Transcriotion-PCR:
• Hyeon et al. 2011. J. Food Prot.
74(10):1756-1761 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Staggemeieretal. 2015. J Virol.
Methods. 213: 65-67 [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA Method 1642

(EPA 820-R-18-001) [Tier III]
or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)
and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

Reoviruses:
Rotavirus (Group A)

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
24 - 72 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

Raynor et al. 2021. PLoS ONE.
16(1): e0244977 [Tier III]

Tissue Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wipes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Park et al. 2015. Appl. Environ.
Microbiol. 81(17): 5987-5992 [Tier
III]

Filter Cassettes: 144-inch2

sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

•	EPA Method 1615 (EPA/600/R-10/181)
[Tier III]

Real-Time Reverse Transcriotion-PCR:

•	Jothikumar et al. 2009. J. Virol.
Methods.

155(2): 126-131 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Staggemeieretal. 2015.

J Virol. Methods. 213: 65-67 [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA Method 1642

(EPA 820-R-18-001) [Tier III]
or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)
and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

SAM Companion - Sample Collection Information Document

C-14

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Protozoa

Cryptosporidium spp.

[Cryptosporidiosis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
96 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA BA Protocol
(EPA/600/R-17/213) [Tier III]



Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Swabs: Sterile 15-mL centrifuae tube
Sponqe-Sticks: Sterile 4-ounce screw-cap

•	Hodges et al. 2010. J. Microbiol.
Methods. 81(2): 141-146 [Tier III]
or

•	Rose et al. 2011. Appl. Environ.
Microbiol. 77(23): 8355-8359 [Tier
III]

or

•	EPA BA Protocol
(EPA/600/R-17/213) [Tier III]

Soonae-Sticks: 100-inch2
Wipes: 144-inch2

specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-proof, and

Procedure;

• Bukhari et al. 2007. Can. J. Microbiol.
53(5): 656-663 [Tier II]

Filter Cassettes: 144-inch2

sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

•	EPA Method 1622

(EPA 815-R-05-001) [Tier I]
or

•	EPA Method 1623.1
(EPA 816-R-12-001) [Tier I]

Soil

50 g - 500 g

50 - 500-mL sterile container

Zopp etal. 2016. Agric. Environ.
Lett. 1:160031 [Tier II]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA Method 1622

(EPA 815-R-05-001) [Tier I]
or

•	EPA Method 1623.1
(EPA 816-R-12-001) [Tier 1]
or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)

•	and

EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

•	Guy et al. 2003. Appl. Environ.
Microbiol. 69(9): 5178-5185
and

•	Jiang et al. 2005. Appl. Environ.
Microbiol. 71(3):

1135-1141 [Tier II]

Entamoeba
histolytica

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch * 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
96 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA BA Protocol
(EPA/600/R-17/213) [Tier III]

Cell Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

•	Hodges et al. 2010. J. Microbiol.
Methods. 81(2): 141-146 [Tier III]
or

•	Rose et al. 2011. Appl. Environ.
Microbiol. 77(23): 8355-8359 [Tier
III]

or

•	EPA BA Protocol
(EPA/600/R-17/213) [Tier III]

Wioes: 144-inch2

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

• Stringert. 1972. J Parasitol. 58(2): 306-
310 [Tier II]

Real-Time PCR:



• Mejia et al. 2013.
Am. J. Trop. Med. Hyg.
88(6): 1041-1047 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Ogbolu et al. 2011. Afr. J. Med. med.
Sci. 40: 85-87 [Tier II]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)
and

•	EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

SAM Companion - Sample Collection Information Document

C-15

April 2023

-------
Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Giardia spp.

[Giardiasis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
96 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA BA Protocol
(EPA/600/R-17/213) [Tier III]

Cell Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Soonae-Sticks: 100-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

•	Hodges etal. 2010. J. Microbiol.
Methods. 81(2): 141-146 [Tier III]
or

•	Rose et al. 2011. Appl. Environ.
Microbiol. 77(23): 8355-8359 [Tier
III]

or

•	EPA BA Protocol
(EPA/600/R-17/213) [Tier III]

Wioes: 144-inch2
Filter Cassettes: 144-inch2

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

•	Keister. 1983.

T. Roy. Soc. Trop. Med. H.
77(4): 487-488 [Tier II]

IMS/FA:

•	EPA Method 1623.1
(EPA 816-R-12-001) [Tier I]

Real-Time PCR:

•	Guy et al. 2003. Appl. Environ.
Microbiol. 69(9): 5178-5185 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Liang and Keeley. 2011. Appl.
Environ. Microbiol. 77(18): 6476-
6485 [Tier III]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA Method 1623.1
(EPA 816-R-12-001) [Tier 1]
or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)

•	and

EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

Naegleria fowleri

[Naegleriasis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Not of Concern10

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wioes: 144-inch2
Filter Cassettes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

Samples should be analyzed as
soon as possible. Holding time of
96 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

•	Hodges et al. 2010. J. Microbiol.
Methods. 81(2): 141-146 [Tier III]
or

•	Rose et al. 2011. Appl. Environ.
Microbiol. 77(23): 8355-8359 [Tier
III]

or

•	EPA BA Protocol
(EPA/600/R-17/213) [Tier III]





•	Standard Method 9750: Naegleria
fowleri [Tier I]

Real-time PCR:

•	Mull et al. 2013. J. Parasitol. Res.
2013: 1-8 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Mull et al. 2013. J. Parasitol. Res.
2013: 1-8 [Tier II]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	Cope et al. 2015. Clin. Infect. Dis.
60(8): e36-42 [Tier II]

or

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)

•	and

EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]

SAM Companion - Sample Collection Information Document

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April 2023

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Sample Collection Information Document - Attachment C

April 2023

Pathogen(s)
[Disease]

Sample Type

Sample Size1

Sample Container2

Holding Time3

Sample Preservation4'5

Sample Processing Methods -
SAM 20226

Selected Analytical Methods - SAM
20226

Toxoplasma gondii

[Toxoplasmosis]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch x 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
96 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

Lass et al. 2020.
Parasitol. 1-11 [Tier II]

Cell Culture:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch

Soonae-Sticks: 100-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

Filter Cassettes: Sterile, leak-oroof. and
sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

•	Hodges etal. 2010. J. Microbiol.
Methods. 81(2): 141-146 [Tier III]
or

•	Rose etal. 2011. Appl. Environ.
Microbiol. 77(23): 8355-8359 [Tier
III]

or

•	EPA BA Protocol (EPA/600/R-
17/213) [Tier III]

Wioes: 144-inch2

• Villegas et al. 2010. J. Microbiol.
Methods. 81(3): 219-225 [Tier II]

Real-Time PCR:



• Yang et al. 2009. Appl. Environ.
Microbiology. 75(11): 3477-3483 [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Escotte-Binet et al. 2019. Vet
Parasitol. 274: 108904 [Tier II]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	Villegas et al. 2010. J. Microbiol.
Methods. 81(3): 219-225 [Tier II]
or

•	EPA Method 1623.1
(EPA 816-R-12-001) [Tier III]



Baylisascaris
procyonis

[Raccoon roundworm
infection]

Air

(airfilters, impingers,
impactor media and
collection fluid)

Flow rates range from 1 - 4
L/minute. Refer to incident- and
site-specific documentation for
duration.

Filters and Imoactor Plates:

4 inch * 6 inch sealable plastic bags

Imoinaer Fluid:

Sterile 50-mL centrifuge tubes

Samples should be analyzed as
soon as possible. Holding time of
96 hours.

Place the sample container
on ice (e.g., ice packs,
secure double-bagged ice).
Temperature should be
maintained at < 10 °C. Do
not allow samples to freeze.

EPA BA Protocol
(EPA/600/R-17/213) [Tier III]

Embrvonation of Eaas and Microscoov:

Surfaces

(swabs, wipes,
Sponge-Sticks and
filter cassettes)

Surface area sampled

Swabs: 4-inch2

Soonae-Sticks: 100-inch2
Wipes: 144-inch2

Swabs: Sterile 15-mL centrifuae tube

Soonae-Sticks: Sterile 4-ounce screw-cao
specimen container

Wioes: Sterile 50-mL centrifuae tube

•	Hodges et al. 2010. J. Microbiol.
Methods. 81(2): 141-146 [Tier III]
or

•	Rose et al. 2011. Appl. Environ.
Microbiol. 77(23): 8355-8359 [Tier
III]

or

•	EPA BA Protocol
(EPA/600/R-17/213) [Tier III]

Filter Cassettes: 144-inch2

sealable 15-mL centrifuge tubes for
containment of the filter nozzle and/or 4 inch x
6 inch sealable plastic bags for containment of
the filter cassette

• Control of Pathogens and Vector
Attraction in Sewage Sludge
(EPA/625/R-92/013) [Tier II]

Soil

50 g - 500 g

50 - 500-mL sterile container

Kazacos. 1983. AM. J. Vet. Res. Vol
44. No. 5:896-900 [Tier II]

•Gatcombe etal. 2010. Parasitol. Res.
106: 499-504 [Tier II]

Water8

(surface water, drinking
water, wastewater and
post decontamination
wastewater)

100 ml_- 100 L

100-mL or larger sterile container

•	EPA and CDC Joint Collection
Protocol (UF)

(EPA 600/R-21/280)

•	and

EPA Method 1642 (Filter
Processing)

(EPA 820-R-18-001) [Tier III]
or

•	Gatcombe etal. 2010. Parasitol.
Res. 106: 499-504 [Tier II]

SAM Companion - Sample Collection Information Document

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April 2023

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Sample Collection Information Document - Attachment C

April 2023

Footnotes:

1 Sample sizes are provided for guidance and may vary depending on the specific contamination incident, data quality objectives and requirements, and laboratory needs. Sampling personnel should consult the incident- and site-specific documentation and/or analytical
laboratory for appropriate sample sizes.

2 Ensure reusable sample containers are autoclavable to allow for sterilization between uses. Sampling personnel should consult the incident- and site-specific documentation and/or analytical laboratory regarding appropriate secondary containment.

3 In all cases, samples to be analyzed for microbiological agents should be transported to the laboratory and analyzed as quickly as possible following collection, in a manner that stabilizes the sample and minimizes the loss of viability.

4Sampling personnel should consult the incident- and site-specific documentation and the analytical laboratory for information regarding requirements for sample preservation (temperature) and transport.

5 For water samples that may have disinfectant residuals, a neutralizing agent (e.g., sodium thiosulfate) should be added to sample containers prior to collection.

6 Methods listed in this column can be located using the SAM website (https://www.epa.aov/esam/selected-analvtical-methods-environmental-remediation-and-recoverv-samV The SAM document is intended to be used concurrently with this Sample Collection Information
Document. Full citations for references not cited and/or accessed through the SAM website are provided in Section 7.0 of this document.

7 If the water sample processing method for bacterial analyses does not address large volume water samples, please refer to the EPA YP Protocol (EPA/600/R-16/109) for ultrafiltration of large volume water samples.

8 For collection and ultrafiltration of large volume water samples in the field, please refer to the EPA and CDC Joint Collection Protocol.

9 Water samples should be processed according to Method 1642 for small volume water samples (e.g., 2 L) or the EPA and CDC Joint Collection Protocol (UF) and Method 1642 (filter processing) for volumes > 10 L.

10 Naegleria fowleri has not been shown to spread via water vapor or aerosol droplets (see CDC's webpage on Naegleria fowleri at https://www.cdc.aov/parasites/naealeria/infection-sources.html").

SAM Companion - Sample Collection Information Document

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April 2023

-------
Attachment D

Sample Collection Information for the
Biotoxin Analytes and Methods Listed in

SAM 2022

[Note: The acronyms and abbreviations used in this
attachment are listed and defined in the beginning of the

report.]

-------
Sample Collection Information Document-Attachment D

April 2023

Attachment D: Sample Collection Information for the Biotoxin Analytes and Methods Listed in SAM 2022

This attachment is intended to be used with SAM 2022 and contains summary information for collection of samples to be analyzed for the selected biotoxins using the methods and procedures listed in SAM.

Holding Time: Minimize transport and storage time. If feasible, analyze or extract immediately upon receipt at the laboratory. Holding time between sample collection and analysis may vary depending on the sample (e.g., sample type, pH).
Sampling personnel should consult the site- and incident-specific documentation and the analytical laboratory for information regarding holding time.

Packaging and Shipping Requirements: The following is general information regarding packaging and shipping. It is the sample originator's responsibility to maintain training and ensure adherence to the most current regulations. Only trained and
certified shippers may ship hazardous materials. Sampling personnel should also consult site- and incident-specific documentation and the receiving laboratory.

Seal container with tape or film and decontaminate the exterior of the container with bleach wipes or decontamination solution. Place in a cooler and package the samples outside the contaminated area. Samples must be packed in a manner that
protects the integrity of the sample containers and provides temperature conditions required for sample preseivation.

Hazardous materials belonging to Class 6, Division 6.1 are packaged according to Packing Groups. Specifications for the non-bulk packaging for Packing Groups I, II, and III are found at 49 CFR 173.201 - 173.203 for liquids, and at 173.211 -
173.213 for solids. For samples meeting the small quantities exception, packaging must meet the minimum standards as described in 49 CFR 173.4.

Sampling personnel should refer to the below websites for additional information regarding packaging and shipping.

•	International Air Transport Association (IATA) (https://www. iata.oro/en/publications/dor/')

•	U.S. Department of Transportation (USDOT) ("https://www.ecfr.aov/current/title-49/subtitle-B/chapter-l/subchapter-C/part-172?toc=1 and https://www.ecfr.aov/current/title-49/subtitle-B/chapter-l/subchapter-C/part-173?toc=1)

•	American Biological Safety Association ('http://www.absa.ora/')

•	World Health Organization Guidance on Regulations for the Transport of Infectious Substances (https://apps.who.int/iris/handle/10665/339825')

•	Federal Select Agent Program (https://www.selectaaents.aov/reaulations/index.htm')

Note: The level of packaging required for samples is dependent on whether the samples are considered hazardous materials. In general, environmental samples which are not considered to pose a significant risk are not subject to the IATA or
USDOT hazardous materials shipping requirements.

Analyte(s)

Sample Type'

Sample Size'

Sample Container

Preservation

Selected Analytical Methods - SAM 2022*

Abrin and

Abrine (abrin marker)

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Impinger: Transfer contents into sterile
leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Avoid freeze-thaw cycles during transport of
samples collected for abrin.

•	Protect from light.

NOTE: For detection and measurement of abrine in
treated water samples, add the following to the
sample container prior to collection:

•	8 mg sodium thiosulfate / 100-mL sample

•	6.4 mg sodium omadine / 100-mL sample

Presumptive:

•	[LFA] Adapted from Biosecurity and Bioterrorism: Biodefense
Strategy, Practice, and Science (2014) 12(1):

49-62 [Tier I/Tier II]

•	as Abrine [LC-MS-MS] EPA 600/R-13/022 [Tier I / Tier II]

•	[ELISA and ECL] Adapted from Journal of Food Protection
(2008) 71(9): 1868-1874 [Tier II]

Confirmatory:

• [LC-MS-MS] Adapted from Analytical Chemistry (2017) 89(21):
11719-11727 [Tier I/Tier II]

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Biological Activity:

• [Enzyme Activity] Adapted from Analytical Biochemistry (2008)
378(1): 87-89 [Tier II]

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

NOTE: Collect water samples for analysis
of abrine in polypropylene container with
polyethylene screw-cap.

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April 2023

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Sample Collection Information Document-Attachment D

April 2023

Analyte(s)

Sample Type111

Sample Size'2'

Sample Container'1'

Preservation

Selected Analytical Methods - SAM 2022*

Aflatoxins

(B1, B2, G1, G2)

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amberglass or amber PETG
containers (or protect from light)

•	1 mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

Presumotive:

•	[Immunoaffinity (column) purification / HPLC-FL] Adapted from
Method 991.31 (AOAC) [Tier II]

•	[Immunoassays (LFA/ELISA)] [Tier III] (No references are
available at this time. Please consult the SAM website* for
information regarding the status of these assays.)

Confirmatorv:

•	[LC-MS-MS] Adapted from Journal of Agricultural and Food
Chemistry (2017) 65(33): 7138-7152 [Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Amanitin

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amberglass or amber PETG
containers (or protect from light)

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

NOTE: For treated water:

•	Add 8 mg sodium thiosulfate and 6.4 mg sodium
omadine / 100-mL sample.

Presumptive:

•	[ELISA] Adapted from Journal of Food Protection (2005) 68(6):
1294-1301 [Tier II]

Presumptive:

•	[LFA] Adapted from Toxins (2020) 12(2): 123 [Tier II]
Confirmatorv:

•	[LC-MS-MS] EPA 600/R-13/022 (EPA) [Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Polypropylene container with polyethylene
screw-cap (protect from light)

Anatoxin-a

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter(3). Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber alass or amber PETG
containers (or protect from light)

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

NOTE: For treated water samples, add 1 g sodium
bisulfate and 0.1 g ascorbic acid / 1-L sample.

Presumotive:

•	[ELISA] Adapted from Inland Waters (2020) 10(1): 109-117
[Tier I / Tier II]

Confirmatorv (Aerosol. Particulate. Solid and Drinkina Water
SamolesV

•	[LC-ESI-MS-MS] Method 545 (EPA) [Tier I / Tier II]

Confirmatorv (Non-Drinkina Water SamolesV

•	[LC-MS-MS] EPA/600/R-17/130 [Tier I]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50-100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

SAM Companion - Sample Collection Information Document

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April 2023

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Sample Collection Information Document-Attachment D

April 2023

An.ilyte(s)

S.imple Type

S.imple Size

S.imple Contiiner

Preservation

Selected An.ilytic.il Methods - SAM 2022'

Botulinum neurotoxins

(Serotypes A - G)

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

•	1 mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Avoid freeze-thaw cycles during transport.

•	Protect from light.

NOTE: For treated water, add 50 mg sodium
thiosulfate / L sample

(DO NOT add sodium thiosulfate to samples that
will be analyzed using MS)

Presumotive:

•	[LFA] Adapted from EPA Environmental Technology Verification
report [Tier 1 / Tier II]

Presumotive:

•	[ECL] Adapted from Journal of the Science of Food and
Agriculture (2014) 94: 707-712 [Tier II]

Presumotive:

•	[Fluorescent bead-based assay] Multi-agency Report. Rapid
Botulinum Toxin Assay Test, Evaluation and Validation Study
Report [Tier III]

Presumptive:

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

•	[Antibody capture FRET-based activity] Adapted from Analytical
Biochemistry (2011) 411: 200-209 [Tier II]

Presumotive:

•	[Immunoassay (B-cell based)] Adapted from Toxins (2018)
10(11): 476 [Tier I / Tier II]

Confirmatorv:

•	[LC-MS-MS / MALDI-TOF MS] Adapted from J. Agric. Food
Chem. (2015) 63(4): 1133-114 [Tier 11]

Bioloaical Activitv:

•	[Mouse Bioassay] APHA Press Compendium of Methods,
Chapter 32 [Tier I]

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Brevetoxins

(A and B Types)

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light

Presumptive:

•	[ELISA] Adapted from Journal of Shellfish Research (2020)
39(2): 491-500 [Tier II]

Confirmatorv:

•	[LC-MS] Adapted from Toxicon (2015) 96: 82-88 [Tier II]

Particulate

(swabs, wipes, filter
cassettes)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile, leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

SAM Companion - Sample Collection Information Document

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April 2023

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Sample Collection Information Document-Attachment D

April 2023

An.ilyte(s)

S.imple Type

S.imple Size

S.imple Contiiner

Preservation

Selected An.ilytic.il Methods - SAM 2022'

a-Conotoxins

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

•	1 mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

Confirmatory:

• [LC-MS] Adapted from Toxins (2017) 9(9): 281
[Tier III]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Cylindrospermopsin

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber alass or amber PETG
containers (or protect from light)

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

NOTE: For treated water, add 1 g sodium bisulfate
and 0.1 g ascorbic acid /1 -L sample.

Presumotive:

•	[ELISA] Adapted from Environmental Sciences and Technology
(2010) 44: 7361-7368 [Tier II]

Confirmatory (Aerosol. Particulate. Solid and Drinkina Water
SamolesV

•	[LC-ESI-MS-MS] Method 545 (EPA) [Tier I / Tier II]

Confirmatorv CNon-drinkina Water SamolesV

•	[LC-MS-MS] EPA/600/R-17/130 [Tier I]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50-100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Amber glass container with fluoropolymer-
lined screw cap

Deoxynivalenol

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

Confirmatorv:

• [LC-MS-MS] Adapted from Journal of Agricultural and Food
Chemistry (2017) 65(33): 7138-7152 [Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile, leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile, leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Sterile, leak proof container - amber glass
or amber PETG (or protect from light)

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Sample Collection Information Document-Attachment D

April 2023

Analyte(s)

Sample Type111

Sample Size'2'

Sample Container'1'

Preservation

Selected Analytical Methods - SAM 2022*

Domoic acid

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

•	1 mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

Presumotive:

•	[ELISA] Adapted from Journal of AOAC International (2007)
90(4): 1011-1027 [Tier II]

•	[ELISA] Adapted from Journal of Shellfish Research (2008)
27(5): 1301-1310 [Tier II]

•	[LFA] [Tier II] (No reference is available at this time. Please
consult the SAM website* for information regarding the status of
this assay.)

Confirmatory;

•	[LC-MS] Adapted from Journal of AOAC International (2014)
97(2): 316-324 [Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

A minimum of 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Fumonisin

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber alass or amber PETG
containers (or protect from light)

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

Confirmatory:

• [LC-MS-MS] Adapted from Journal of Agricultural and Food
Chemistry (2017) 65(33): 7138-7152 [Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Amber glass container with fluoropolymer-
lined screw cap

Microcystins

(LA, LF, LR, LY, RR, YR)

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C; do not freeze]

•	Protect from light.

NOTE:

Prior to collection, add the following to containers
used to collect treated water samples:

•	For samples to be analyzed using ELISA (Method
546), 100 mg sodium thiosulfate /1 -L sample.

•	For samples to be analyzed using LC-MS-MS
(Method 544), add 1) 7.75 g Trizma, 2) 2 g 2-
chloroacetamide, 3) 100 mg ascorbic acid, 4) 0.35 g
ethylenediamine-tetraacetic acid trisodium salt / 1-L
sample.

Presumotive:

• [ELISA] Method 546 (EPA) [Tier I / Tier II]
Confirmatory (Drinkina Water SamolesV

leak proof container for shipping.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

•	[LC-MS-MS] Method 544 (EPA) [Tier I]

Confirmatory (Aerosol. Particulate. Solid and Non-Drinkina Water
SamolesV

•	[LC-MS-MS] EPA/600/R-17/344 [Tier I / Tier II]

Bioloaical Activity:

•	[PP2A Activity Assay] Adapted from Toxins (2019) 11(12): 729
[Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50-100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

500 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

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April 2023

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Sample Collection Information Document-Attachment D

April 2023

An.ilyte(s)

S.imple Type

S.imple Size

S.imple Contiiner

Preservation

Selected An.ilytic.il Methods - SAM 2022'

Ochratoxin A

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber alass or amber PETG
containers (or protect from light)

•	1 mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

Confirmatory:

• [LC-MS-MS] Adapted from Journal of Agricultural and Food
Chemistry (2017) 65(33): 7138-7152 [Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Amber glass container with fluoropolymer-
lined screw cap

Picrotoxin

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

Confirmatory:

• [LC-UV] Adapted from Journal of Pharmaceutical and
Biomedical Analysis (1989) 7(3): 369-375 [Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

SAM Companion - Sample Collection Information Document

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April 2023

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Sample Collection Information Document-Attachment D

April 2023

An.ilyte(s)

S.imple Type

S.imple Size

S.imple Contiiner

Preservation

Selected An.ilytic.il Methods - SAM 2022'

Ricin and

Ricinine (ricin marker)

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile plastic leak proof
container.

Imoinaer: Transfer contents into sterile
plastic leak proof container.

NOTE: Glass containers should not be
used if analyzing samples using TRF. Use
amber PETG containers (or protect from
light)

•	1 mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Avoid freeze-thaw cycles during transport of
samples collected for ricin.

•	Protect from light.

NOTE: For treated water, add 50 mg sodium
thiosulfate / 1-L sample

NOTE: For samples collected for detection and
measurement of ricinine in treated water, add the
following to the sample container prior to collection:

•	8 mg sodium thiosulfate / 100-mL sample

•	6.4 mg sodium omadine / 100-mL sample

Presumptive:

•	[LFA] Adapted from Biosecurity and Bioterrorism: Biodefense
Strategy, Practice, and Science (2013) 11(4): 237-250 [Tier I]

•	[ELISA] Adapted from Journal of Food Protection (2005) 68(6):
1294-1301 [Tier II]

•	[ECL] EPA/600/R-22/033A [Tier I / Tier II]

•	as Ricinine [LC-MS-MS] EPA 600/R-13/022 (EPA/CDC)

[Tier I/Tier II]

•	[TRF] CDC LRN**

Confirmatory:

•	[Immunocapture / LC-MS-MS] Adapted from Analytical
Chemistry (2011) 83: 2897-2905 [Tier I / Tier II]

Bioloaical Activitv:

[Immunocapture / MALDI-TOF MS] Adapted from Analytical
Chemistry (2016) 88:6867-6872 [Tier I / Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber PETG
(or protect from light)

NOTE: Glass containers should not be
used if analyzing samples using TRF.

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber PETG
(or protect from light)

NOTE: Glass containers should not be
used if analyzing samples using TRF.

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - plastic-
amber PETG (or protect from light)

NOTE: Glass containers should not be
used if analyzing samples using TRF.

Saxitoxins

(STX, NEO, dcSTX,
dcNEOSTX, doSTX,
GTX 1-6, dcGTX 1-4)

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

NOTE: For treated water, add 100 - 1,000 mg/L
sodium thiosulfate or 100 mg/L ascorbic acid

NOTE: For untreated freshwater samples to be
analyzed using ELISA kits, 10X concentrated
diluent is added to the sample container prior to or
immediately following sample collection.

Presumptive:

• [Receptor binding assay] Method 2011.27 (AOAC) [Tier II]
Presumotive (Aerosol. Particulate and Solid SamolesV

leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

• [ELISA] Adapted from Toxicon (2009) 54: 313-320 [Tier II]

Presumptive (Water SamolesV
¦ [ELISA] Harmful Algae (2016) 56: 77-90 [Tier I]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes or
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

• [HI LIC MS-MS] Adapted from Journal of Chromatography A
(2015) 1387: 1-12 [Tier II]

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

A minimum of 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

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Sample Collection Information Document-Attachment D

April 2023

An.ilyte(s)

S.imple Type

S.imple Size

S.imple Contiiner

Preservation

Selected An.ilytic.il Methods - SAM 2022'

Shiga and Shiga-like
Toxins

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

•	1 mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Avoid freeze-thaw cycles during transport.

•	Protect from light.

Presumotive:

•	[ELISA] Adapted from Austin Immunology (2016)

1(2): 1007: 1-7 [Tier I / Tier II]

Confirmatorv:

•	[LC-MS-MS] Adapted from Analytical Chemistry (2014) 86: 4698-
4706 [Tier II]

Particulate
(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Staphylococcal
enterotoxins

(SEA, SEB, SEC, SED,
SEE)

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Avoid freeze-thaw cycles during transport.

•	Protect from light.

Presumptive:

•	[ELFA] Method 2007.06 (AOAC) [Tier II]

•	[ECL] Adapted from Journal of AOAC International (2014)
97(3): 862-867 [Tier III]

Confirmatorv:

•	[ELISA] Adapted from Letters in Applied Microbiology (2011)
52: 468-474 [Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

T-2 Mycotoxin

(T-2, HT-2)

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

NOTE: For treated water, add 8 mg sodium
thiosulfate and 6.4 mg sodium omadine / 100-mL
sample

Presumptive:

•	[ELISA] Adapted from Journal of Food Protection (2005) 68(6):
1294-1301 [Tier II]

Confirmatorv:

•	[LC-MS] Adapted from Rapid Communications in Mass
Spectrometry (2006) 20(9): 1422-1428 [Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

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Sample Collection Information Document-Attachment D

April 2023

Analyte(s)

Sample Type111

Sample Size(2>

Sample Container11'

Preservation

Selected Analytical Methods - SAM 2022*

Tetrodotoxin

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Impinqer: Transfer contents into sterile

•	1 mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

Presumptive:

• [Receptor binding assay] Method 2011.27 (AOAC) [Tier II]
Confirmatory:

leak proof container.

NOTE: Use amber glass or amber PETG
containers (or protect from light)

• [LC-MS-MS] Adapted from Journal of AOAC International (2017)
100(5): 1469-1482 [Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Zearalenone

Aerosol

(air filter, filter cassette,
liquid impinger)'3'

See Footnote (4)

Filter: Sterile MCE or PTFE filter.131 Place
the filter in a sterile leak proof container.

Imoinaer: Transfer contents into sterile
leak proof container.

NOTE: Use amber alass or amber PETG
containers (or protect from light)

•	I mmediately place the sample container on ice
(e.g., ice packs, secure double-bagged ice). Target
temperature < 10°C.

•	Protect from light.

Confirmatory:

• [LC-MS-MS] Adapted from Journal of Agricultural and Food
Chemistry (2017) 65(33): 7138-7152 [Tier II]

Particulate

(swab, wipe, filter
cassette)

At least 2 sterile, synthetic,
and moistened wipes,
swabs, or filter cassettes

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Solid

(soil, powder)

50- 100 mL

Sterile leak proof container - amber glass
or amber PETG (or protect from light)

Water

(surface water, waste
water, drinking water)

100 mL

Amber glass container with fluoropolymer-
lined screw cap

Footnotes:

(1)	The sample types listed are included in SAM 2022. Sampling personnel should consult the incident- and site-specific documentation and/or analytical laboratory regarding appropriate sample types and secondary containment. The use of amber
sample containers and/or shielding sample containers from light is noted. Although many biotoxins have not been explicitly studied with regard to photodegradation, many may be subject to photodegradation based on their structure, it may be
appropriate to minimize light exposure for all biotoxins.

(2)	Sample sizes are provided for guidance, and may vary depending on the specific contamination incident, data quality objectives and requirements, and laboratory needs. If requested by the laboratory, additional sample(s) must be collected for
analyses using multiple methods, or for laboratory quality control analyses (e.g., duplicates, matrix spikes). It is also recommended that additional sample(s) be collected in cases where low concentrations are expected or in the case of an
anticipated need for reanalysis due to sample spillage or unforeseen analytical difficulties.

(3)	Information regarding the types of filters used for collection of aerosol samples to be analyzed for toxins is limited. Sampling personnel should consult the incident- and site-specific documentation and/or analytical laboratory to determine the
appropriate air collection filter(s).

(4)	Volumes of collected air samples are dependent on the concentration of the toxin and the collection device used. Sampling personnel should consult the incident- and site-specific documentation and/or analytical laboratory for appropriate
volumes.

* Methods listed in this column can be located using the SAM website ("https://www.epa.aov/esam/selected-analvtical-methods-environmental-remediation-and-recoverv-samy Full citations for references not cited and/or accessed through this
document or its website are provided in Section 7.0. Analytical technologies and methods addressing biotoxins continue to be developed and improved; the contact information on this website should be used for updates regarding analytical
procedures.

**A standardized procedure, reagents and agent-specific algorithms are available only to LRN member laboratories.

SAM Companion - Sample Collection Information Document

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April 2023

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&EPA

United States
Environmental Protection
Agency

PRESORTED STANDARD
POSTAGE & FEES PAID
EPA

PERMIT NO. G-35

Office of Research and Development
Homeland Security Research Program
Cincinnati, OH 45268

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