*>EPA

United States
Environmental Protection
Agency

Double Bottom Test Tube

Inventors: Elizabeth Sams
US Patent 11.090.64?

TRL 4

Double Bottom Test Tub with sample swab enclosed

Context

Recent events have only emphasized the importance of public health monitoring for disease control. Saliva
can be used as an effective, non-invasive, self-administered tool for monitoring population health. Under
standard monitoring conditions a sample swab is placed into a test tube with an ordinary flat lid after sampling.
When the sample is shipped to a laboratory for testing, a technician must open the tube and invert the sample
swab so that the swab is at the top of the tube for centrifugation and sample/swab separation.

Summary

EPA researcher Sams invented a double bottom test tube to overcome many of the issues associated with
sample processing from standard, flat lid test tubes. The double bottom test tube eliminates the need for
manual inversion of sample swabs, saving time and reducing the risk of sample contamination by eliminating
the need to open the tube.

The double-bottom test tube is inexpensive, simple to use, and can be safely mailed to homes. It can also
collect at least ImL of fluid and can withstand centrifuge forces of up to 2500 revolutions per minute. In
laboratory testing, the streamlined flipping process saved 22 minutes for every 20 tubes processed, an
average savings of 1.1 minutes per tube. Over 10,000 tubes, a lab could eliminate more than 180 hours of
labor costs.

Potential Applications

•	Healthcare and Population Monitoring

•	Research and Development

•	Consumer Test Kits

Contact

Meghan Sheehan, JD. CLP
Federal Technology Transfer Act (FTTA) Specialist

www, epa. qov/ftta

FTTA Home Page

U.S. Environmental Protection Agency
Office of Research and Development


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