"SERI\ United States Office of Chemical Safety and Environmental Protection Agency Pollution Prevention Final Risk Evaluation for Asbestos Part 1: Chrysotile Asbestos Systematic Review Supplemental File: Data Quality Evaluation of Environmental Fate and Transport Studies December 2020 ------- Table of Contents Favero-Longo, SE; Turci, F; Tomatis, M; Castelli, D; Bonfante, P; Hochella, MF; Piervittori, R; Fubini, B. (2005). Chrysotile asbestos is progressively converted into anon-fibrous amorphous material by the chelating action of lichen metabolites. J Environ Monit 7: 764- 766. http://dx.doi.org/10.1039/b507569f. HERO ID: 3520647 3 Schreier, H; Omueti, JA; Lavkulich, LM. (1987). Weathering Processes Of Asbestos- Rich Serpentinitic Sediments. Soil Sci Soc Am J 51: 993-999. HERO ID: 1917037 6 Speil, S; Leineweber, JP. (1969). Asbestos minerals in modern technology. Environ Res 2: 166-208. HERO ID: 5353620 8 Gronow, JR. (1987). The dissolution of asbestos fibres in water. Clay Miner 22: 21-35. http://dx.doi.org/10.1180/claymin.1987.022.L03. HERO ID: 5353542 11 Choi, I; Smith, RW. (1972). Kinetic study of dissolution of asbestos fibers in water. J Colloid Interface Sci 40. http://dx.doi.org/10.1016/0021-9797(72)90014-8. HERO ID: 4140459 13 Bales, RC; Morgan, JJ. (1985). Surface-charge And Adsorption Properties of Chrysotile Asbestos in Natural-Waters. Environ Sci Technol 19: 1213-1219. HERO ID: 3582724 15 Belanger, SE; Schurr, K; Allen, DJ; Gohara, AF. (1986). Effects of chrysotile asbestos on coho salmon and green sunfish: evidence of behavioral and pathological stress. Environ Res 39: 74-85. HERO ID: 3584231 17 Belanger, SE; Cherry, DS; Cairns, J. (1986). Uptake of chrysotile asbestos fibers alters growth and reproduction of Asiatic clams. Can J Fish Aquat Sci, 43: 43-52. HERO ID: 3093600 20 Belanger, SE; Cherry, DS; Cairns J, JR. (1986). Seasonal behavioral and growth changes of juvenile Corbicula-fluminea exposed to chrysotile asbestos. Water Res 20: 1243-1250. HERO ID: 3093856.... 23 Belanger, SE; Cherry, DS; Cairns, J; McGuire, MJ. (1987). Using Asiatic clams as abiomonitor for chrysotile asbestos in public water supplies. J Am Waterworks Assoc 79: 69-74. http://dx.doi.Org/10.1002/j.1551-8833.1987.tb02817.x.HEROID: 3584230 25 Belanger, SE; Cherry, DS; Cairns, J. (1990). Functional and pathological impairment of japanese medaka (oryzias-latipes) by long-term asbestos exposure. Aquat Toxicol 17: 133-154. HERO ID: 3585046 28 Page 2 of 30 ------- Study Reference: Favero-Longo, SE; Turci, F; Tomatis, M; Castelli, D; Bonfante, P; Hochella, MF; Piervittori, R; Fubini, B. (2005). Chrysotile asbestos is progressively converted into a non- fibrous amorphous material by the chelating action of lichen metabolites. J Environ Monit 7: 764- 766. htti>://dx.doi.on.'/10.11l39/b5(l7569f. HERO ID: 3520647 Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test Substance Identity High Common name and chemical formula provided. 1 2 2 2. Test Substance Purity Medium Purity not provided but not likely to influence the study results. 2 1 2 Test Design 3. Study Controls High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 4. Test Substance Stability Medium Some details regarding the test substance homogeneity, preparation, and storage conditions were not reported but their omission is not likely to influence the study results. 2 1 2 Test Conditions 5. Test Method Suitability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 6. Testing Conditions High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 7. Testing Consistency High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 8. System Type and Design High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Page 3 of 30 ------- Study Reference: Favero-Longo, SE; Turci, F; Tomatis, M; Castelli, D; Bonfante, P; Hochella, MF; Piervittori, R; Fubini, B. (2005). Chrysotile asbestos is progressively converted into a non- fibrous amorphous material by the chelating action of lichen metabolites. J Environ Monit 7: 764- 766. htti>://dx.doi.or«/10.11l39/b5(l7569f. HERO ID: 3520647 Test Organisms 9. Test Organism Degradation High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 10. Test Organism Partitioning Not rated The metric is not applicable to this study type. NR NR NR Outcome Assessment 11. Outcome Assessment Methodology High The ability of oxalic acid to transform chrysotile asbestos into an amorphous material was the desired outcome of interest and the methodology used was appropriate for that outcome. 1 1 1 12. Sampling Methods High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Confounding/ Variable Control 13. Confounding Variables High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 14. Outcomes Unrelated to Exposure Not rated The metric is not applicable to this study type. NR NR NR Data Presentation and Analysis 15. Data Reporting High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 16. Statistical Methods and Kinetic Calculations High Statistical methods were described in the Supporting Information of the publication and met the criteria for high confidence 1 1 1 Other 17. Verification or Plausibility of Results High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Page 4 of 30 ------- Study Reference: Favero-Longo, SE; Turci, F; Tomatis, M; Castelli, D; Bonfante, P; Hochella, MF; Piervittori, R; Fubini, B. (2005). Chrysotile asbestos is progressively converted into a non- fibrous amorphous material by the chelating action of lichen metabolites. J Environ Monit 7: 764- 766. htti>://dx.doi.on.'/10.11l39/b5(l7569f. HERO ID: 3520647 18. QSAR Models Not rated The metric is not applicable to this study type. NR NR NR Sum of scores: 17 20 22 Mid. Medium l.nu Overall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 1.1 Overall Score (Rounded): 1.1 1 and 1 >1.7 and <2.3 2 ' ;md ' Overall Quality Level: High1 1 Some test details were obtained from the supporting material that was available in HERO (ID: 5353622s) Page 5 of 30 ------- Study Reference: Schreier, H; Omueti, JA; Lavkulich, LM. (1987). Weathering Processes of Asbestos- Rich Serpentinitic Sediments. Soil Sci Soc Am J 51: 993-999. HERO ID: 1917037 Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test Substance Identity High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 2. Test Substance Purity High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Test Design 3. Study Controls High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 4. Test Substance Stability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Test Conditions 5. Test Method Suitability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 6. Testing Conditions High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 7. Testing Consistency High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 8. System Type and Design High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Test Organisms 9. Test Organism Degradation Not rated The metric is not applicable to this study type. NR NR NR 10. Test Not rated The metric is not NR NR NR Page 6 of 30 ------- Study Reference: Schreier, H; Omueti, JA; Lavkulich, LM. (1987). Weathering Processes of Asbestos- Rich Serpentinitic Sediments. Soil Sci Soc Am J 51: 993-999. HERO ID: 1917037 Organism Partitioning applicable to this study type. Outcome Assessment 11. Outcome Assessment Methodology High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 12. Sampling Methods Not rated The metric is not applicable to this study type. NR NR NR Confounding/ Variable Control 13. Confounding Variables High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 14. Outcomes Unrelated to Exposure Not rated The metric is not applicable to this study type. NR NR NR Data Presentation and Analysis 15. Data Reporting High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 16. Statistical Methods and Kinetic Calculations High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Other 17. Verification or Plausibility of Results High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 18. QSAR Models Not rated The metric is not applicable to this study type. NR NR NR Sum of scores: 13 17 17 II mh Medium I.HW Overall Score = Sum of Weighted Scores/Sum of Metric \\ righting Factors: 1 Overall Score (Rounded): 1 1 ;iih.I 1 1 " and <2.3 2 'and ' Overall Quality Level: High Page 7 of 30 ------- Study Reference: Speil, S; Leineweber, JP. (1969). Asbestos minerals in modern technology. Environ Res 2: 166-208. HERO ID: 5353620 Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test Substance Identity High The test substance was identified by chemical name. 1 2 2 2. Test Substance Purity Medium The test substance source was not reported nor was the purity stated; however, the omissions were not likely to have a substantial impact on the study results. 2 1 2 Test Design 3. Study Controls Not rated The metric is not applicable to this study type. NR NR NR 4. Test Substance Stability Medium The test substance stability, homogeneity, preparation or storage conditions were not reported; however, these factors were not likely to influence the test substance or were not likely to have a substantial impact on study results. 2 1 2 Test Conditions 5. Test Method Suitability Medium Details of the method were only summarized but are not likely to have a substantial impact on the results. 2 1 2 6. Testing Conditions Unacceptable Testing conditions were not reported, and data provided were insufficient to interpret results. 4 2 8 Page 8 of 30 ------- Study Reference: Speil, S; Leineweber, JP. (1969). Asbestos minerals in modern technology. Environ Res 2: 166-208. HERO ID: 5353620 7. Testing Consistency Medium Some test conditions across samples or study groups were not reported, but these discrepancies were not likely to have a substantial impact on study results. 2 1 2 8. System Type and Design Unacceptable Details were not reported preventing meaningful interpretation of study results. 4 1 4 Test Organisms 9. Test Organism Degradation Not rated The metric is not applicable to this study type. NR NR NR 10. Test Organism Partitioning Not rated The metric is not applicable to this study type. NR NR NR Outcome Assessment 11. Outcome Assessment Methodology Unacceptable Study details were not reported to evaluate methodology. 4 1 4 12. Sampling Methods Medium Details regarding sampling methods of the outcome(s) were not fully reported but were unlikely to have major impact on the results. 2 1 2 Confounding/ Variable Control 13. Confounding Variables Not rated The metric is not applicable to this study type. NR NR NR 14. Outcomes Unrelated to Exposure Not rated The metric is not applicable to this study type. NR NR NR Page 9 of 30 ------- Study Reference: Speil, S; Leineweber, JP. (1969). Asbestos minerals in modern technology. Environ Res 2: 166-208. HERO ID: 5353620 Data Presentation and Analysis 15. Data Reporting Medium The target chemical and transformation product(s) concentrations, extraction efficiency, percent recovery, or mass balance were not reported; however, these omissions were not likely to have a substantial impact on study results. 2 2 4 16. Statistical Methods and Kinetic Calculations Not rated The metric is not applicable to this study type. NR NR NR Other 17. Verification or Plausibility of Results High Reported values were within expected range. 1 1 1 18. QSAR Models Not rated The metric is not applicable to this study type. NR NR NR Sum of scores: 26 14 33 Mid. Medium Low Overall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 2.36 Overall Score (Rounded): 4 1 ;iikI 1 1 " and : ^ 2 ' and ' Overall Quality Level: Unacceptable1 1 Due to limited information assessing the results was challenging. Page 10 of 30 ------- Study Reference: Gronow, JR. (1987). The dissolution of asbestos fibres in water. Clay Miner 22: 21-35. hUi>s://(loi.orc/l0.l180/clavmin. 1987.022.1.03 HERO ID: 5353542 Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test Substance Identity High The test substance was identified by common name. 1 2 2 2. Test Substance Purity Medium The test substance source was reported. The purity of the test substance was not reported but this is unlikely to influence the study results. 2 1 2 Test Design 3. Study Controls Not rated The metric is not applicable to this study type. NR NR NR 4. Test Substance Stability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Test Conditions 5. Test Method Suitability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 6. Testing Conditions High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 7. Testing Consistency High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 8. System Type and Design High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Test Organisms 9. Test Organism Degradation Not rated The metric is not applicable to this study type. NR NR NR Page 11 of 30 ------- Study Reference: Gronow, JR. (1987). The dissolution of asbestos fibres in water. Clay Miner 22: 21-35. htti)s://doi.oru/10.1180/clavmin. 1987.022.1.03 HERO ID: 5353542 10. Test Organism Partitioning Not rated The metric is not applicable to this study type. NR NR NR Outcome Assessment 11. Outcome Assessment Methodology High The outcome assessment methodology addressed the outcome of interest. 1 1 1 12. Sampling Methods High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Confounding/ Variable Control 13. Confounding Variables High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 14. Outcomes Unrelated to Exposure Not rated The metric is not applicable to this study type. NR NR NR Data Presentation and Analysis 15. Data Reporting High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 16. Statistical Methods and Kinetic Calculations High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Other 17. Verification or Plausibility of Results High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 18. QSAR Models Not rated The metric is not applicable to this study type. NR NR NR Sum of scores: 14 16 17 Mid. Medium I.HW Overall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 1.06 Overall Score (Rounded): 1.1 1 and 1 1 " and : ^ C", -a C^, c-j A1 Overall Quality Level: High Page 12 of 30 ------- Study Reference: Choi, I; Smith, RW. (1972). Kinetic study of dissolution of asbestos fibers in water. J Colloid Interface Sci 40. htti>://(ix.(ioi.on'/10.1016/tHI21 9797f72)9tHI14-8. HERO ID: 4140459 Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test Substance Identity High The test substance was identified by chemical name. 1 2 2 2. Test Substance Purity Medium Source indicated but purity was not reported; however, the omission was not likely had to have an impact on the study results. 2 1 2 Test Design 3. Study Controls Not rated The metric is not applicable to this study type. NR NR NR 4. Test Substance Stability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Test Conditions 5. Test Method Suitability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 6. Testing Conditions High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 7. Testing Consistency High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 8. System Type and Design High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Test Organisms 9. Test Organism Degradation Not rated The metric is not applicable to this study type. NR NR NR 10. Test Organism Partitioning Not rated The metric is not applicable to this study type. NR NR NR Page 13 of 30 ------- Study Reference: Choi, I; Smith, RW. (1972). Kinetic study of dissolution of asbestos fibers in water. J Colloid Interface Sci 40. htti>://(ix.(ioi.on'/10.1016/tHI21 9797f72)9tHI14-8. HERO ID: 4140459 Outcome Assessment 11. Outcome Assessment Methodology Medium There were minor differences between the outcome assessment methodology and the outcome of interest; however, the difference does not likely have a substantial impact on the study results. 2 1 2 12. Sampling Methods High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Confounding/ Variable Control 13. Confounding Variables High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 14. Outcomes Unrelated to Exposure Not rated The metric is not applicable to this study type. NR NR NR Data Presentation and Analysis 15. Data Reporting High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 16. Statistical Methods and Kinetic Calculations High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Other 17. Verification or Plausibility of Results High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 18. QSAR Models Not rated The metric is not applicable to this study type. NR NR NR Sum of scores: 15 16 18 Mid. .Medium I.HW Overall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 1.12 Overall Score (Rounded): 1.1 1 ;iih.I 1 1 "and : ^ 2 'and ' Overall Quality Level: High Page 14 of 30 ------- Study Reference: Bales, RC; Morgan, JJ. (1985). Surface-charge And Adsorption Properties of Chrysotile Asbestos in Natural-Waters. Environ Sci Technol 19: 1213-1219. HERO ID: 3582724 Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test Substance Identity High The test substance was identified by common name. 1 2 2 2. Test Substance Purity Medium The purity of the original ore was not provided; however, this omission was not likely to impact on the results. 2 1 2 Test Design 3. Study Controls Not rated The metric is not applicable to this study type. NR NR NR 4. Test Substance Stability High The test substance is stable under the test conditions. 1 1 1 Test Conditions 5. Test Method Suitability High The test method was suitable for the test substance. 1 1 1 6. Testing Conditions High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 7. Testing Consistency High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 8. System Type and Design Medium Some system and design information were not reported, but the omissions were not likely to impact on the results. 2 1 2 Test Organisms 9. Test Organism Degradation Not rated The metric is not applicable to this study type. NR NR NR 10. Test Organism Partitioning Not rated The metric is not applicable to this study type. NR NR NR Page 15 of 30 ------- Study Reference: Bales, RC; Morgan, JJ. (1985). Surface-charge And Adsorption Properties of Chrysotile Asbestos in Natural-Waters. Environ Sci Technol 19: 1213-1219. HERO ID: 3582724 Outcome Assessment 11. Outcome Assessment Methodology High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 12. Sampling Methods High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Confounding/ Variable Control 13. Confounding Variables High No confounding variables were reported. 1 1 1 14. Outcomes Unrelated to Exposure Not rated The metric is not applicable to this study type. NR NR NR Data Presentation and Analysis 15. Data Reporting High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 16. Statistical Methods and Kinetic Calculations High Calculations were described and assumptions used in their interpretation were also stated. 1 1 1 Other 17. Verification or Plausibility of Results High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 18. QSAR Models Not rated The metric is not applicable to this study type. NR NR NR Sum of scores: 15 16 18 Mid. Medium I.HM Overall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 1.12 Overall Score (Rounded): 1.1 1 :ind 1 " 1 "and : ^ 2 ' and ' Overall Quality Level: High Page 16 of 30 ------- Study Reference: Belanger, SE; Schurr, K; Allen, DJ; Gohara, AF. (1986). Effects of chrysotile asbestos on coho salmon and green sunfish: evidence of behavioral and pathological stress. Environ Res 39: 74-85. HERO ID: 3584231 Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test Substance Identity High The substance was identified by common name. 1 2 2 2. Test Substance Purity Medium Purity and source of test substance not provided; however, it is not likely to affect the results of this study. 2 1 2 Test Design 3. Study Controls High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 4. Test Substance Stability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Test Conditions 5. Test Method Suitability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 6. Testing Conditions High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 7. Testing Consistency High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 8. System Type and Design High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Page 17 of 30 ------- Study Reference: Belanger, SE; Schurr, K; Allen, DJ; Gohara, AF. (1986). Effects of chrysotile asbestos on coho salmon and green sunfish: evidence of behavioral and pathological stress. Environ Res 39: 74-85. HERO ID: 3584231 Test Organisms 9. Test Organism Degradation High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 10. Test Organism Partitioning Not rated The metric is not applicable to this study type. NR NR NR Outcome Assessment 11. Outcome Assessment Methodology High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 12. Sampling Methods High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Confounding/ Variable Control 13. Confounding Variables High Confounding variables were appropriately addressed. 1 1 1 14. Outcomes Unrelated to Exposure High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Data Presentation and Analysis 15. Data Reporting High Data reporting was appropriate for this study. 1 2 2 16. Statistical Methods and Kinetic Calculations High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Other 17. Verification or Plausibility of Results High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 18. QSAR Models Not rated The metric is not applicable to this study type. NR NR NR Sum of scores: 17 21 22 Midi Medium l.mx Overall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 1.05 Overall Score (Rounded): 1 Page 18 of 30 ------- Study Reference: Belanger, SE; Schurr, K; Allen, DJ; Gohara, AF. (1986). Effects of chrysotile asbestos on coho salmon and green sunfish: evidence of behavioral and pathological stress. Environ Res 39: 74-85. HERO ID: 3584231 hind 1." 1 " ;ind : ^ : ^ ;md ^ Overall Quality Level: High Page 19 of 30 ------- Study Reference: Belanger, SE; Cherry, DS; Cairns, J. (1986). Uptake of chrysotile asbestos fibers alters growth and reproduction of Asiatic clams. Can J Fish Aquat Sci, 43: 43-52. HERO ID: 30936(10 Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test Substance Identity High CAS number was not provided; however, the common name of the substance was used. 1 2 2 2. Test Substance Purity High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Test Design 3. Study Controls High Blank groups were used which contained no asbestos and validated the test results. 1 2 2 4. Test Substance Stability High The test substance preparation was reported and appropriate for the study. 1 1 1 Test Conditions 5. Test Method Suitability High The test method was suitable for the purpose of the study. 1 1 1 6. Testing Conditions High Testing conditions were clearly reported and suitable for the study. 1 2 2 7. Testing Consistency High Test conditions were consistent across study groups. 1 1 1 8. System Type and Design High The system described was able to maintain substance concentrations. 1 1 1 Test Organisms 9. Test Organism Degradation Not rated The metric is not applicable to this study type. NR NR NR 10. Test Organism Partitioning High The test organism was described and appropriate for the study type 1 2 2 Page 20 of 30 ------- Study Reference: Belanger, SE; Cherry, DS; Cairns, J. (1986). Uptake of chrysotile asbestos fibers alters growth and reproduction of Asiatic clams. Can J Fish Aquat Sci, 43: 43-52. HERO ID: 30936(10 Outcome Assessment 11. Outcome Assessment Methodology High The outcome assessment methodology was described clearly and reported the desired outcome. 1 1 1 12. Sampling Methods Medium Accumulation rates were not derived due to sampling being done only at the end of the trials; however, this limitation did not have a substantial impact on the results. 2 1 2 Confounding/ Variable Control 13. Confounding Variables High No confounding variables were noted among study groups that would influence the outcome assessment. 1 1 1 14. Outcomes Unrelated to Exposure High Exposure to asbestos caused decreased siphoning activity in all organisms to some degree. However, no health effects unrelated to exposure were noted. 1 1 1 Data Presentation and Analysis 15. Data Reporting Medium The method for measuring asbestos fiber levels in the whole-body homogenate, gill tissue, and visceral tissue were clearly described; however, no data was presented to demonstrate the accuracy of those methods. Considering that they were based on previously established methods in other literature, it is unlikely that this impacted the study results. 2 2 4 16. Statistical Methods and Kinetic Calculations High Statistical analysis was described and included in the results. 1 1 1 Page 21 of 30 ------- Study Reference: Belanger, SE; Cherry, DS; Cairns, J. (1986). Uptake of chrysotile asbestos fibers alters growth and reproduction of Asiatic clams. Can J Fish Aquat Sci, 43: 43-52. HERO ID: 30936(10 Other 17. Verification or Plausibility of Results High The reported values were reasonable. 1 1 1 18. QSAR Models Not rated The metric is not applicable to this study type. NR NR NR Sum of scores: 18 21 24 II mh Medium l.im Overall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 1.14 Overall Score (Rounded): 1.1 1 and 1." 1 "and 2 ^ 2 'and ' Overall Quality Level: High Page 22 of 30 ------- Study Reference: Belanger, SE; Cherry, DS; Cairns J, JR. (1986). Seasonal behavioral and growth changes of juvenile Corbicula-fluminea exposed to chrysotile asbestos. Water Res 20: 1243-1250. HERO ID: 3093856 Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test Substance Identity High CAS number was not provided; however, common name was used. 1 2 2 2. Test Substance Purity Medium Test substance purity was not stated but is not likely to impact the study results. 2 1 2 Test Design 3. Study Controls High Blank groups were used and had no detectable levels of asbestos 1 2 2 4. Test Substance Stability High The test substance is stable under testing conditions 1 1 1 Test Conditions 5. Test Method Suitability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 6. Testing Conditions High Testing conditions were clearly reported and suitable for the study method. 1 2 2 7. Testing Consistency High Reported testing conditions were consistent across study groups. 1 1 1 8. System Type and Design High The system type and design were capable of maintaining test substance concentrations. 1 1 1 Test Organisms 9. Test Organism Degradation Not rated The metric is not applicable to this study type. NR NR NR 10. Test Organism Partitioning High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 Outcome Assessment 11. Outcome Assessment High This metric met the criteria for high 1 1 1 Page 23 of 30 ------- Study Reference: Belanger, SE; Cherry, DS; Cairns J, JR. (1986). Seasonal behavioral and growth changes of juvenile Corbicula-fluminea exposed to chrysotile asbestos. Water Res 20: 1243-1250. HERO ID: 3093856 Methodology confidence as expected for this type of study. 12. Sampling Methods High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Confounding/ Variable Control 13. Confounding Variables High There were no confounding differences among the study groups that influenced the outcomes. 1 1 1 14. Outcomes Unrelated to Exposure High No health outcomes unrelated to exposure were noted. 1 1 1 Data Presentation and Analysis 15. Data Reporting High The analytical method was suitable for detecting the test substance levels. 1 2 2 16. Statistical Methods and Kinetic Calculations High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Other 17. Verification or Plausibility of Results High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 18. QSAR Models Not rated The metric is not applicable to this study type. NR NR NR Sum of scores: 17 21 22 1 liuli Medium l.nu Overall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 1.05 Overall Score (Rounded): 1 1 ;iihJ 1 1 "and : ^ 2 'and ' Overall Quality Level: High Page 24 of 30 ------- Study Reference: Belanger, SE; Cherry, DS; Cairns, J; McGuire, MJ. (1987). Using Asiatic clams as a biomonitor for chrysotile asbestos in public water supplies. J Am Water Works Assoc 79: 69-74. HERO ID: 3584230 Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test Substance Identity High The substance was identified by common name. 1 2 2 2. Test Substance Purity High The purity of the original ore was not provided; however, the authors noted that fibers were only identified as chrysotile if the characteristic TEM transmission patterns were seen and were considered "nonchrysotile fibers" if not. 1 1 1 Test Design 3. Study Controls High Blank groups were included in the laboratory exposure experiments and did not have detectable levels of asbestos. 1 2 2 4. Test Substance Stability High The test substance is stable under the test conditions. 1 1 1 Test Conditions 5. Test Method Suitability High The test method was suitable for the test substance. 1 1 1 6. Testing Conditions Medium Some testing conditions were omitted such as temperature and pH ranges, but these omissions are not likely to have a substantial impact on the results. 2 2 4 7. Testing Consistency High There were no noted inconsistencies between the study groups. 1 1 1 Page 25 of 30 ------- Study Reference: Belanger, SE; Cherry, DS; Cairns, J; McGuire, MJ. (1987). Using Asiatic clams as a biomonitor for chrysotile asbestos in public water supplies. J Am Water Works Assoc 79: 69-74. httDs://doi.ore/10.1002/i.l551-8833.1987.tb02817.x HERO ID: 3584230 8. System Type and Design High The system was capable of maintaining the concentration of the test substance. 1 1 1 Test 9. Test Not rated The metric is not NR NR NR Organisms Organism Degradation applicable to this study type. 10. Test Organism High The test organism was described and was 1 2 2 Partitioning suitable for the study type. Outcome 11. Outcome High The outcome 1 1 1 Assessment Assessment Methodology assessment methodology addressed the outcomes of interest. 12. Sampling Methods High The sampling methods were adequate for the purpose of the study. 1 1 1 Confounding/ Variable Control 13. Confounding Variables High There were no confounding differences among study groups that influenced the outcome assessment. Also, uncertainty was provided in the concentration measurements for both the field and laboratory experiments. 1 1 1 14. Outcomes High There were no 1 1 1 Unrelated to differences in health Exposure outcomes between groups unrelated to exposure. Data 15. Data Medium No standard reference 2 2 4 Presentation and Analysis Reporting material was analyzed in order to test the accuracy of the analysis method; however, this omission was not likely to have had a substantial impact on the results. Page 26 of 30 ------- Study Reference: Belanger, SE; Cherry, DS; Cairns, J; McGuire, MJ. (1987). Using Asiatic clams as a biomonitor for chrysotile asbestos in public water supplies. J Am Water Works Assoc 79: 69-74. httDs://doi.ore/10.1002/i.l551-8833.1987.tb02817.x HERO ID: 3584230 16. Statistical Methods and Kinetic Calculations High Calculations were described clearly, and assumptions used in their interpretation were also stated. 1 1 1 Other 17. Verification or Plausibility of Results High The study results were compared to similar experiments done with other test organisms and were reasonable in comparison. 1 1 1 18. QSAR Models Not rated The metric is not applicable to this study type. NR NR NR Sum of scores: 18 21 25 1 liuli Medium 1 A»\X ()\ erall Score = Sum of Weighted Scores/Sum of Metric Weighting Factors: 1.19 Overall Score (Rounded): 1.2 1 III id 1 1 "and 2 ^ 2 'and ' Overall Quality Level: High Page 27 of 30 ------- Study Reference: Belanger, SE; Cherry, DS; Cairns, J. (1990). Functional and pathological impairment of japanese medaka (oryzias-latipes) by long-term asbestos exposure. Aquat Toxicol 17: 133- 154. HERO ID: 3585046 Domain Metric Qualitative Determination [i.e., High, Medium, Low, Unacceptable, or Not rated] Comments Metric Score Metric Weighting Factor Weighted Score Test Substance 1. Test Substance Identity High The substance was identified using common name. 1 2 2 2. Test Substance Purity Medium The purity of the original ore was not provided; but not likely to impact study results. 2 1 2 Test Design 3. Study Controls High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 4. Test Substance Stability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Test Conditions 5. Test Method Suitability High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 6. Testing Conditions High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 7. Testing Consistency High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 8. System Type and Design High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Test Organisms 9. Test Organism Degradation Not rated The metric is not applicable to this study type. NR NR NR Page 28 of 30 ------- Study Reference: Belanger, SE; Cherry, DS; Cairns, J. (1990). Functional and pathological impairment of japanese medaka (oryzias-latipes) by long-term asbestos exposure. Aquat Toxicol 17: 133- 154. HERO ID: 3585046 10. Test Organism Partitioning High The test organism was described and was suitable for the study type. 1 2 2 Outcome Assessment 11. Outcome Assessment Methodology High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 12. Sampling Methods High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 Confounding/ Variable Control 13. Confounding Variables High There were no confounding differences among study groups that influenced the outcome assessment. 1 1 1 14. Outcomes Unrelated to Exposure High There were no differences in health outcomes between groups unrelated to exposure. 1 1 1 Data Presentation and Analysis 15. Data Reporting High This metric met the criteria for high confidence as expected for this type of study. 1 2 2 16. Statistical Methods and Kinetic Calculations High Calculations were described clearly, and assumptions used in their interpretation were also stated. 1 1 1 Other 17. Verification or Plausibility of Results High This metric met the criteria for high confidence as expected for this type of study. 1 1 1 18. QSAR Models Not rated The metric is not applicable to this study type. NR NR NR Sum of scores: 17 21 22 Midi Medium I.HW ()\ erall Score = Sum of Weighted Soires/Sum of Metric \\ cighting Factors: 1.05 Overall Score (Rounded): 1 Page 29 of 30 ------- Study Reference: Belanger, SE; Cherry, DS; Cairns, J. (1990). Functional and pathological impairment of japanese medaka (oryzias-latipes) by long-term asbestos exposure. Aquat Toxicol 17: 133- 154. HERO ID: 3585046 1 ;ind 1 1 -;ind : ' : ^ ;ind ^ Overall Quality Level: High Page 30 of 30 ------- |