United States Environmental Protection Agency ECOTOX Knowledgebase Virtual Training Breakout Group Worksheet Case Study Level 1 This worksheet was developed for the Breakout Group session of the ECOTOX Knowledgebase Virtual Training, hosted by the U.S. Environmental Protection Agency's Center for Computational Toxicology and Exposure on May 17, 2022. For more information about the ECOTOX Knowledgebase tool— • Visit the ECOTOX Knowledgebase at www.epa.gov/ecotox. • Contact ECOTOX support at ecotox.support@epa.gov or by calling 218-529-5225. • Read more in Olker et al. 2022, https://doi.org/10.10Q2/etc.5324. To identify records available in ECOTOX for a specific chemical AND species group. Directions 1. Select a chemical from the list provided on Page 5 of this worksheet. 2. Type the chemical and its CAS Registry Number in the space below. [chemical] [CAS Registry Number(s)] 3. Because we are starting with a chemical and have no other parameters, should you start with Search or Explore? [note Search or Explore] 4. Create a Custom Group for your chemical. Then refer to the list of Query Filters on the left-hand side to walk through the next set of questions. 5. How many Species Groups have records in ECOTOX for the selected compound(s)? [note number] 6. Refine the results by selecting a specific Species Group. Which group did you select? [species group selected] Goal l ------- *>EPA United States Environmental Protection Agency /C?s fA/owv.^0 7. Based on the Species Group you selected, limit the results to either "Aquatic" or "Terrestrial" at the top of the left-hand side. Which one did you select? [habitat selected] 8. Change the view as needed (i.e., Group Summary, Records, Plot View) to answer the following questions: a. Change your results to Records view. b. What types of effects were observed in the Species Group you focused on? [effects populated in ECOTOX] c. Change your results to Plot View. d. What were the lowest concentrations for which these effects were reported? [concentration values reported in ECOTOX] e. What paper or papers were these lowest concentrations reported in? [paper title/citation from the Google Scholar link that opens in a new tab] f. Write down the ECOREF number (identifier for the reference) by hovering over the reference column. [ECOREF number] g. Select Send Query Filters to Search at the top of the right-hand side. This will open Search in a new tab. Be sure to click the green Update Search button on the bottom-left. h. Modify the Publications Options to limit the output to the reference identified above. How many records are displayed? [number of records available for your chemical and species group of reference] 2 ------- United States Environmental Protection Agency i. Use the Customize Output Fields feature to add fields to the output table (e.g., Control, Gender, Experimental Design, and EE Comment). What did you choose? [additional field used to further refine results] j. Did the authors of the above paper(s) report what types of controls were used? If so, what were the types of controls? [yes/no; controls] k. Were you able to find/access the publication to read/review the original results? Did you encounter challenges or have questions about the results? [yes/no; challenges or questions] I. Expand the Publications Options to include all references that meet the search parameters (no longer limiting results to the single identified reference). Now how many records are displayed? [number of records] m. Export and save the results as an Excel file and filter them to show the records from the reference with the lowest effect concentrations discussed in the previous steps. n. Now, try to find the same list of papers by using the Search module. Begin by adding the chemical name or CAS Registry Number and species group to the parameters on the left- o. Did you find that Explore or Search was an easier starting point for your query? List one example of why. [one preference for using Explore or Search in this scenario] hand side. 3 ------- United States Environmental Protection Agency Reflection 1. In what case example from your work environment would ECOTOX be useful? 2. What have you learned about the process and workflow used to find information and papers in ECOTOX? 3. What challenges did you encounter, and how did you solve them? 4 ------- United States Environmental Protection »* Agency Chemical List Category Compound(s) CAS Registry Number(s) Pesticide/Herbicide Atrazine 1912-24-9 Pesticide/Herbicide Roundup 38641-94-0 Pesticide/Herbicide Chlorpyrifos 2921-88-2 Metal Cadmium 7440-43-9, 10108-64-2, plus 11 others in the Cadmium group Metal Lead 7439-92-1, 7758-95-4, 301-04-2, 10099-74-8, plus 9 others in the Lead group Metal Selenium 35 unique compounds in the Selenium group Pharmaceutical/Personal Care Product 17a-Ethinylestradiol 57-63-6 Pharmaceutical/Personal Care Product Clofibrate/Clofibric Acid 637-07-0, 882-09-7 Pharmaceutical/Personal Care Product DEET 134-62-3 Species Groups Vertebrates Invertebrates Plants and Other Fish Insects/Spiders Flowers, Trees, Shrubs, Ferns Mammals Worms Algae Birds Molluscs Fungi Amphibian Crustaceans Moss, Hornworts Reptiles Other Invertebrates Miscellaneous (Communities, Higher Taxonomic Level) 5 ------- |