November 2009 Web-based Interspecies Correlation Estimation (Web-ICE) for Acute Toxicity: User Manual Version 3.0 http: //www. epa. g o v/cea m p u b l/fcha i n/web ice/ Sandy Raimondo, Deborah N. Vivian, and Mace G. Barron U.S Environmental Protection Agency Office of Research and Development National Health and Environmental Effects Research Laboratory Gulf Ecology Division Gulf Breeze, FL 32561 ------- Reference Web-ICE as: Raimondo, S., D.N. Vivian, and M.G. Barron. 2009. Web-based Interspecies Correlation Estimation (Web-ICE) for Acute Toxicity: User Manual. Version 3.0. Office of Research and Development, U. S. Environmental Protection Agency. Gulf Breeze, FL. Disclaimer: The information in this document has been reviewed in accordance with U.S. Environmental Protection Agency policy and approved for publication. Approval does not signify that the content reflects the views of the Agency, nor does mention of trade names or products constitute endorsement or recommendation for use. ------- Contents Abstract 3 Introduction 4 Methods 5 I. Database Development 5 Aquatic (Fish and Invertebrates) 5 Wildlife (Birds and Mammals) 6 II. Model Development 6 III. Model Validation 7 Using the Web-ICE Program 8 I. Working with Web-ICE Aquatic or Web-ICE Wildlife Modules 9 Selecting Model Taxa 9 Estimating Toxicity 10 II. The Species Sensitivity Distribution (SSD) Module 11 Generating an SSD: 14 III. The Endangered Species Module 14 Producing an Endangered Species Toxicity Report 14 IV. Accessing Model Data 16 Guidance for Model Selection and Use 17 I. Statistical Definitions 17 II. Selecting a Model with Low Uncertainty 18 Rules of Thumb 18 Surrogate Species Selection: An Example 19 III. Evaluating Model Predictions 19 IV. Selecting Predicted Toxicity Values for SSDs 20 V. Applying Web-ICE in Ecological Risk Assessment (ERA) 20 Acknowledgements 22 References 22 Appendices 25 1 ------- Appendix I. Summary of acceptance requirements for data included in ICE models 25 Appendix II. List of Species in Aquatic Database 27 III. List of Species in Wildlife Database 31 2 ------- Abstract Predictive toxicological models are integral to ecological risk assessment because data for most species are limited. Web-based Interspecies Correlation Estimation (Web-ICE) models are least square regressions that predict acute toxicity (LC50/LD50) of a chemical to a species, genus, or family based on estimates of relative sensitivity between the taxa of interest and that of a surrogate species. Web-ICE 3.0 includes a total 1440 models for aquatic taxa and 852 models for wildlife taxa. For aquatic species within the same family, Web-ICE models predict within 5-fold and 10- fold of the actual value with 91 % and 96% certainty, respectively. For two species within the same order, aquatic models predict within 5-fold and 10-fold of the actual value with 86% and 96% certainty, respectively. Overall for wildlife species, Web-ICE predicts toxicity within 5-fold of the actual value with 85% certainty and within 10-fold of the actual value with 95% certainty. Models predict within 5-fold and 10-fold of the actual value with 90 and 97% certainty for wildlife surrogate and predicted taxa within the same order. For both aquatic and wildlife taxa, model certainty increases with decreasing taxonomic distance. Web-ICE 3.0 improves on earlier versions with the inclusion of an endangered species module, improved functionality of the SSD module, and more rigorous standardization of toxicity data. 3 ------- Introduction Information on the acute toxicity to multiple species is needed for the assessment of the risks to, and the protection of, individuals, populations, and ecological communities. However, toxicity data are limited for the majority of species, while standard test species are generally data rich. To address data gaps in species sensitivity, the Interspecies Correlation Estimations (ICE) application was developed by the U.S. Environmental Protection Agency (US EPA) and collaborators to extrapolate acute toxicity to taxa with little or no acute toxicity data, including threatened and endangered species (Asfaw et al. 2003). Web-based Interspecies Correlation Estimations (Web-ICE) provides interspecies extrapolation models for acute toxicity in a user-friendly internet platform. ICE models estimate the acute toxicity (LC50/LD50) of a chemical to a species, genus, or family with no test data (the predicted taxon) from the known toxicity of the chemical to a species with test data (the surrogate species). ICE models are least square regressions of the relationship between surrogate and predicted taxon based on a database of acute toxicity values: median lethal water concentrations for aquatic species (LC50; |ig/L) and median lethal oral doses for wildlife species (LD50; mg/kg bodyweight). ICE models can be used to estimate acute toxicity when a toxicity is known for a surrogate species or it can be estimated (e.g., QSAR), and there is an existing ICE model between the surrogate and taxa of interest (e.g., species-species; species-genus; species-family). In addition to direct toxicity estimation from a surrogate species to predicted taxa, Web-ICE contains a Species Sensitivity Distribution (SSD) module that estimates the toxicity of all predicted species available for a common surrogate. Acute toxicity values generated by Web-ICE are expressed as a logistic cumulative probability distribution function in the SSD module to estimate an associated Hazardous Concentration (HC) or Hazardous Dose (HD) (Dyer et al. 2006). For example, the HC5 corresponds to the 5th percentile of the log-logistic species sensitivity distribution and is assumed to be protective of 95% of tested species. ICE-generated SSD hazard levels have been shown to be within an order of magnitude of measured HC5s (Dyer et al. 2006, Dyer et al. 2008) and HD5s (Awkerman et al. 2008) and provide additional information for ecological risk assessment. This manual provides step-by-step instructions for using Web-ICE, as well as information on the expanded databases, model development, model validation, and user guidance on model selection and interpretation. User guidelines outlined in the Guidance for Model Selection and Use section of this manual should be followed to ensure high confidence and low uncertainty in model predictions used in risk assessment. Web-ICE 3.0 improves on earlier versions with the inclusion of an endangered species module, improved functionality of the SSD module, and more rigorous standardization of toxicity data. 4 ------- Methods I. Database Development Aquatic (Fish and Invertebrates) The database of acute toxicity used in development of ICE models included 5501 EC/LC50 values of 180 species and 1266 chemicals. The database was compiled from the following EPA1 and public domain sources: • US EPA ECOTOX (http://cfpub.epa.gov/ecotox/; accessed February 2009), • US EPA Office of Pesticide Programs excotoxicity database (accessed January 2007) • US EPA Office of Water Ambient Water Quality Criteria • US EPA OPPT PreManufacture Notification (PMN) • US EPA OPPT High Production Volume (HPV) Challenge Program • US EPA Office of Research and Development data sources • Mayer and Ellersieck 1986 • Open literature (for list of references, see Raimondo et al. 2008, 2009) Data used in model development adhered to standard acute toxicity test condition requirements of the American Society for Testing and Materials (ASTM 2007, and earlier editions) and the US EPA Office of Prevention, Pesticides, and Toxic Substances (US EPA 1996). Data were standardized for test conditions and organism life stage to reduce variability (Appendix I). In short, selection criteria for aquatic test data were as follows: • Reported chemical name or structure with chemical active ingredient > 90% • Open-ended toxicity values (i.e. > 100 mg/kg or <100 mg/kg) were excluded • Endpoint was death (LC50) or immobilization (EC50) • 48h EC/LC50 for daphnids, midges and mosquitoes; 96h EC/LC50 for fish and all other invertebrates • Juvenile only for fish, amphibians, insects, molluscs, decapods; all life stages for other groups (Raimondo et al. 2009) • Water quality parameters reported for test condition (e.g. temperature, salinity) or confirmation that test conditions met appropriate guideline conditions (e.g. GLP, previously reviewed OPP ecotoxicity data) • Water quality parameters provided for normalization of metals, ammonia and pentachlorophenol as directed by Ambient Water Quality Criteria (AWQC; US EPA 1986) 1 All confidential business information (CBI) and data have been censored. 5 ------- When there was more than one toxicity value reported from multiple sources for a species and chemical, the geometric mean of the values were used. In cases where the range of minimum and maximum values for a chemical and species were greater than 10-fold, all data records for that chemical were removed for that species due to their high variability. Toxicity test values for specific compounds were normalized according to Ambient Water Quality Criteria procedures (e.g., specific metals adjusted to 50 mg/L hardness; reported on element basis; pentachlorophenol and ammonia were temperature and pH normalized; US EPA 1986). The resulting aquatic database was used to develop models to predict toxicity to a species, genus, or family from a surrogate species (see Appendix II). Wildlife (Birds and Mammals) The wildlife database was comprised of 4329 acute, single oral dose LD50 values (mg/kg body weight) for 156 species and 951 chemicals. The data were collected from the open literature (Hudson et al. 1984; Shafer and Bowles 1985, 2004; Shafer et al. 1983; Smith 1987) and from datasets compiled by governmental agencies of the United States (US EPA) and Canada (Environment Canada) (Baril et al. 1994; Mineau et al. 2001). Data were standardized by using only data for adult animals and data for chemicals of technical grade or formulations with > 90% active ingredient. Open-ended toxicity values (i.e. > 100 mg/kg or <100 mg/kg) and duplicate records among multiple sources were not included in model development. When data were reported as a range (ie. 100-200 mg/kg; Hudson et al. 1984) or data were collected from multiple sources for a species and chemical, the geometric mean of the values was used. In cases where the range of minimum and maximum values for a chemical and species were greater than 10-fold, all data records for that chemical were removed for that species due to their high variability. Models derived from this wildlife database may be used to predict toxicity to a species or family from a surrogate species. Genus level models were not developed from the wildlife database because there were limited genera that had two or more species (See Appendix III), which is requirement for development of higher taxa models. II. Model Development Models were developed using least squares methodology in which both variables are independent and subject to measurement error (Asfaw et al. 2003). For species- level models developed from aquatic and wildlife databases, an algorithm was written in S-plus (Insightful 2001) to pair every species with every other species by common chemical. Three or more common chemicals per pair were required for inclusion in the analysis. For each species pair, a linear model was used to calculate the regression equation Log-io(predicted toxicity) = a + b*l_og-io(surrogate toxicity), where a and &are the intercept and slope of the line, respectively. Genus (aquatic only) and family-level models were similarly developed by pairing each surrogate species with each genus or family by common chemical. Predicted genera and families required unique toxicity values for two or more species within the taxon. Toxicity values for the surrogate species were removed in cases where it was compared to its own genus or family. ICE 6 ------- models were only developed between two aquatic taxa or two wildlife taxa; there are no models to predict toxicity to aquatic taxa from a wildlife species, or vice versa. Only models that had a significant relationship (p-value < 0.05) are included in Web-ICE. The following summarizes the number of significant models developed from the aquatic and wildlife databases for different taxonomic levels: 1) Aquatic species: 782 models comparing 77 species to 77 species; 2) Aquatic genera: 287 models comparing 63 species to 28 genera; 3) Aquatic family: 371 models comparing 69 species to 27 families; 4) Wildlife species: 560 models comparing 49 species to 49 species; 5) Wildlife family: 292 models comparing 49 species to 16 families. III. Model Validation The uncertainty of each model was assessed using leave-one-out cross- validation (Insightful 2001). In this method, each pair of acute toxicity values for surrogate and predicted taxa were systematically removed from the original model. The remaining data were used to rebuild a model and estimate the toxicity value of the removed predicted taxa toxicity value from the respective surrogate species toxicity value. This method could only be used for models with degrees of freedom equal or greater than 2 (N > 4). To maintain uniformity among the large number of models contained within Web-ICE, the "N-fold" difference among each estimated and actual value was calculated and used to determine the fitness of the estimated toxicity value. For aquatic species, inter-laboratory variation of acute toxicity test data for a given species and chemical can be as great as a 5-fold difference (Fairbrother 2008). For wildlife species, the average range of multiple toxicity measurements for a specific chemical and species was determined to be between 4.0 and 6.4 (Raimondo et al. 2007). Thus, a 5-fold difference was deemed a good fit in the validation analysis of both aquatic and wildlife models. The cross-validation success rate was calculated for each model as the proportion of removed data points that were predicted within 5-fold of the actual value from models that were statistically significant. In cases where the removal of a xy data pair resulted in the development of a model that was not significant at the p < 0.05 level, these replicates were not included in the cross-validation success rate. This is because models that are not significant at the p<0.05 level have a greater risk of Type I error. This was only the case for models with low degrees of freedom (<8) and a p-value between 0.01 and 0.05 in the original model. There is a strong relationship between taxonomic distance and cross-validation success rate, with uncertainty increasing with larger taxonomic distance (Raimondo et al., 2007). In aquatic species, models predict within 5-fold and 10-fold of the actual value with 91 and 96% certainty for surrogate and predicted taxa within the same family, and for 86 and 96% within the same order. In wildlife species, models predict within 5-fold and 10-fold of the actual value with 90 and 97% certainty for surrogate and predicted taxa within the same order. Model certainty decreases with increasing taxonomic distance. A more detailed account of model uncertainty as it relates chemical mode of action/class is discussed in Raimondo et al. (2007). 7 ------- Using the Web-ICE Program The Web-ICE platform contains separate modules that predict acute toxicity to aquatic (vertebrates and invertebrates) species, genera, or families (ICE Aquatic) and wildlife (terrestrial birds and mammals) species or families (ICE Wildlife) (Figure 1). The Species Sensitivity Distribution Module is available for aquatic and wildlife species and batch processes species level toxicity from all entered surrogates. The Endangered Species Module, also available for aquatic and wildlife taxa, predicts toxicity to listed species from all available species, genus, or family level models for the entered surrogates. Each module is accessible from either the home page or from the blue navigation bar along the left side of the page. Before working with a Web-ICE module, you must first decide if you are going to work with aquatic or wildlife taxa, the program does not contain models that estimate wildlife toxicity from an aquatic surrogate, or vice versa. IS N THE ISSUES I SCIENCE & TECHNOLOGY I LAWS & REGULATIONS I ABOUT EPA SEARCH Interspecies Correlation Estimation Eacontactiis @share You are here: EPA Home » Exposure Assessment » Food Chain » WeblCE »Interspecies Correlation Estimation Exposure Assessment Models Web-ICE Home Aquatic Species Aquatic Genus Aquatic Family Wildlife Species Wildlife Family Species Sensitivity Distributions Endangered Species Aquatic Wildlife Basic Information Download Model Data Bibliography The Web-based Interspecies Correlation Estimation (Web-ICE) application estimates acute toxicity to aquatic and terrestrial organisms for use in risk assessment. Please refer to the User Manual for detailed instructions on using Web-ICE Web-ICE Modules ICE Aquatic Aquatic vertebrates invertebrates Species Genus Family ICE Wildlife TerrestriaJ Birds Mammals Species Family Species Sensitivity Distribution Module ICE Aquatic ICE Wildlife Endangered Species Module ICE Aquatic Please address all comments and questions to the webmaster Office of Research and Development National Health and Environmental Effects Research Laboratory Cuff Ecology Division Figure 1. Home page of Web-ICE program 8 ------- I. Working with Web-ICE Aquatic or Web-ICE Wildlife Modules Selecting Mode! Taxa 1. From either the home page or the blue navigation bar, click the link for the module with which you will be working (Aquatic species, genus, or family; Wildlife species or family). 2. You will then be directed to a Taxa Selection Page (Figure 2) which will allow you to select your surrogate and predicted taxa for the model. 3. You may search for your surrogate and predicted taxa by either common name or scientific name by selecting the appropriate option in the Sort by: drop down menu. The default is set to common name. 4. From the drop down menus, select the surrogate species and predicted taxon. It does not matter which you select first; however, the second choice is limited to the models available for the taxon chosen first. 5. To change any of your selections, press Reset and start again. 6. Click Continue to be directed to the calculator page for toxicity estimation. If there is not a model for your predicted species of interest, you will need to use a genus or family-level model to predict toxicity. The available models may be determined by browsing through the genus (aquatics only) and family level modules, or by searching through the spreadsheets of model information available through the Download Model Data option on the blue navigation bar. The downloadable Microsoft Excel® spreadsheets provided for each Web-ICE module may be sorted by surrogate species or predicted taxa to identify available models. 9 ------- =,EPA United States Environmental Protection Agency • ALL EPA O THIS AREA Advanced iearch N THE ISSUES I SCIENCE & TECHNOLOGY I LAWS a REGULATIONS I ABOUT EPA Interspecies Correlation Estimation E3Comaa Us o Share You are here: EPA Home » Exposure Assessment» Food Chain » '.VeblCE »Aquatic Species Taxa Selection Exposure Assessment Models Web-ICE Home Aquatic Species Aquatic Genus Aquatic Family Wildlife Species Wildlife Family Species Sensitivity Distributions Endangered Species Basic Information Download Model Data Bibliography Aquatic Species - Taxa Selection Page Surrogate: | Rainbow trout (Oncorhynchus mykiss) Sort By | Common Name Reset | "Hi irown trout (balmo trutt Continue Please address all comments and questions to the webmaster Office of Research and Development National Health and Environmental Effects Research Laboratory Culf Ecology Division Figure 2, Taxa selection page Estimating Toxicity The surrogate and predicted species selected from the previous page are listed at the top of a calculator page (Figure 3). This page is divided into four parts: input, calculated results, model statistics, and model graphic . The known toxicity for the surrogate species is entered under Surrogate Acute Toxicity, below which the desired confidence limits can be selected (Figure 3A). Predicted toxicity estimates and confidence intervals are displayed under Predicted Acute Toxicity (Figure 3B). The bottom left side of the page contains the model statistics (Figure 3C). Please refer to the Statistical Definitions section of this manual for more specific information. The graph shows the data (LC50/LD50 values) used to develop the model, the regression line (straight inner line), and 95% confidence intervals (curved outer lines) (Figure 3D). The surrogate and predicted taxa are labeled on the X and Y axes, respectively. Both the model statistics and the graph are unique for each model and will change for each surrogate species and predicted taxon. 1. Enter the acute toxicity value in the box located under Surrogate Acute Toxicity (Figure 3A). 2. Select your desired confidence interval (90, 95, or 99%) from the drop down menu located under Select Confidence Interval (Figure 3A). The default for the confidence intervals is 95%. 10 ------- 3. Press Calculate 4. The calculated values will appear in the three boxes labeled Predicted Acute Toxicity, Lower Limit and Upper limit (Figure 3B). 5. Log-transformed values of the surrogate and predicted toxicity values appear in parentheses next to the values. 6. If the entered surrogate toxicity value is outside the range of toxicity values used to develop the model, a pop-up with the warning "This value is outside the x-axis range for this model. Continue?" will appear. The user may select "OK" to proceed to calculate the toxicity value or hit cancel to enter another value. 7. To select a different model, select the link to the desired module in the blue navigation bar on left side of the page. LEARN THE ISSUES I SCIENCE & TECHNOLOGY I LAWS & REGULATIONS I ABOUT EPA Interspecies Correlation Estimation Seaman Us You are here: EPA Home » Exposure Assessment » Food Chain » WeblCE » Aquatic Species Taxa Selection » Calculator Calculator - Aquatic Species Surrogate Species: Rainbow trout (Oticorhynchus mykiss) Predicted Species: Brown trout (Salmo trutta) Surrogate Acute Toxicity (log value) Predicted Acute Toxicity (log value) |150 uq, L (2.17} 142.71 pg,L(2.15) Select Confidence Interval: A Lower Limit Upper Limit 195% 104.10 Mg.'L || 195.65 pg/L B Calculate Model Information Intercept: Slope: Z"1 Degrees of Freedom (N-2): R2: p-value: Average value of surrogate (log value): 0.042271 0.970642 17 0.964248 0.000000 119.80 (2.07) Minimum value of surrogate (log value): 0.163864 (-0.785515) Maximum value of surrogate (log value): 1 7808.08 (4.25) Mean Square Error (MSE): 0.079728 Sum of Squares (Sxx): 38.80 Cross-validation Success (%): 94.73 Taxonomic Distance: 2 Oncorhynchus mykiss (Log LC50) -igure 3, Calculator Page II. The Species Sensitivity Distribution fSSD) Module Species Sensitivity Distributions (SSDs) are probabilistic models that describe the sensitivity of biological species to a chemical. SSDs generated in Web-ICE are log- logistic cumulative distribution functions of toxicity values for multiple species (de Zwart 2002) and are used to estimate a hazard level (hazardous concentration (HC) or hazardous dose (HD)) that is protective of most test species (e.g. 95%) by estimating 11 ------- the concentration or dose at a corresponding percentile (e.g. 5th) of the distribution (Dyer et al. 2006). The SSD modules for aquatic and wildlife species generate SSDs from Web-ICE toxicity values estimated from one or more surrogate species. Toxicity values for one or more surrogate species are used to simultaneously estimate toxicity to all possible predicted species with existing Web-ICE models. The SSD is then generated using all estimated toxicity values and the entered toxicity of the surrogate species. Toxicity values for up to 25 surrogate species may be entered (Figure 4). If more than one surrogate species estimates toxicity to the same predicted species, Web-ICE selects the toxicity value with the smallest confidence intervals. If multiple surrogates are used and a predicted value is estimated for one of the surrogate species, Web-ICE uses the entered value for that species and excludes the predicted value(s) from the SSD. An HC/HD level is automatically calculated from the distribution. The user can deselect toxicity values for predicted species that they wish to exclude from the SSD by clicking on the box to the left of the predicted species (Figure 5), and the associated HC/HD value is automatically recalculated. An HC/HD drop down menu on the output page allows the user to specify the hazard level to calculate. HC1/HD1 corresponds to the 1st percentile, HC5/HD5 corresponds to the 5th percentile, and HC10/HD10 corresponds to the 10th percentile. The default is set to HC5 for aquatic species and HD5 for wildlife species. Web-ICE uses the SSD described by the logistic distribution function of de Zwart (2002): F(C)= 1/(1 + exp ((a-C)/p)) The logio-transformed environmental concentration (or dose) of the evaluated chemical is represented by C, the parameter, a, is the sample mean of the log™ -transformed toxicity values and p is defined as V3/71 * o, where o is the standard deviation of the log-10 -transformed toxicity values (de Zwart 2002). The HC/HD level is determined as the percentile of interest (e.g. 5th) of the described distribution. Corresponding SSDs are also developed from the upper and lower confidence limits of the predicted toxicity values and are used to calculate the upper and lower bounds of the HC/HD value at a given percentile. For example, the lower bound of the HC5 is calculated as the 5th percentile of the SSD developed from the estimated lower confidence limit of each predicted toxicity value. Similarly, the upper bound of an HC5 is calculated as the 5th percentile of the SSD developed from the estimated upper limit of each predicted toxicity value. 12 ------- &EPA ^^1*1 wm United States Environmental Protection Agency LEARN THE ISSUES I SCIENCE & TECHNOLOGY I LAWS & REGULATIONS I ABOUT EPA • ALL EPA 9 THIS AREA Advanced Search I ¦ SEARCH Interspecies Correlation Estimation E3ContactUs Share You are here: EPA Home » Exposure Assessment » Food Chain » WeblCE » Species Sensistivity Distributions » Aquatic Species Exposure Assessment Models Web-ICE Home Aquatic Species Aquatic Genus Aquatic Family Wildlife Species Wildlife Family Species Sensitivity Distributions Endangered Species Aquatic Wildlife Basic Information User Manual Download Model Data Bibliography Species Sensitivity Distributions - Aquatic Species Multiple Surrogate SSD Surrogate: r Sort By | Common Name H Species Fathead minnow (Pimephales promelas) Bluegill (Lepomis macrochirus) Daphnid (Daphnia magna) Sheepshead minnow (Cyprinodon variegatus) Calculate SSD I Toxicity' |150 Remove Species ] |125 Remove Species |75 Remove Species 1100 Remove Species Please address all comments and questions to the webmaster Office of Research and Development National Health and Environmental Effects Research Laboratory Culf Ecology Division News by Email ' • ) Widgets Figure 4. SSD taxa selection page. &EPA, i n United States Environmental Protection Agency LEARN THE ISSUES I SCIENCE & TECHNOLOGY I LAWS & REGULATIONS I ABOUT EPA • ALL EPA P THIS AREA Advanced Searc )' Hj SEARCH Interspecies Correlation Estimation BlConactUs @share You are here: EPA Home » Exposure Assessment » Food Chain » WeblCE » Aquatic Species » Results Exposure Assessment Models Web-ICE Home Aquatic Species Aquatic Genus Aquatic Family Wildlife Species Wildlife Family Species Sensitivity Distributions Endangered Species Aquatic Wildlife Basic Information User Manual Download Model Data Bibliography Species Sensitivity Distributions - Aquatic Surrogate Species: Fathead minnow (Pimephales promelas), Bluegill (Lepomis macrochirus), Daphnid (Daphnia magna), Sheepshead minnow (Cyprinodon variegatus) Input Toxicity: 1 50, 125, 75, 100 ug L |HC5 8.44 ug, L 95% Confidence Interval: 1.44 - 21.62 Common Name Scientific Sort Sort W Stonefly P Amphipod W' Stonefly P Daphnid Sort 1 Claassenia sabulosa 1.38 Hyalella azteca 3.58 Pteronarcella badia 3.66 Ceriodaphnia dubia 8.20 Estimated 95% Show Data: Toxicity Confidence | Surrogate W Amphipod Gammarus lacustris 8.26 P Chinook salmon Oncorhynchus 13.41 tshawytscha P Amphipod Gammarus 18.39 pseudolimnaeus 17 Shortnose Acipenser 32.82 sturgeon brevirostrum P Mvsid Americamvsis bahia 39.30 Figure 5. SSD output page. Intervals Sort 0.460 - 4.1.3 Fathead minnow (Pimephales promelas) 0.048 - Daphnid (Daphnia magna) 266.24 2.15 - 6.21 Fathead minnow (Pimephales promelas) 0.769 - Fathead minnow (Pimephales 87.56 promelas) 1.71 - 39.90 Bluegill (Lepomis macrochirus) 1.63 - Bluegill (Lepomis macrochirus) 110.04 6.29 - 53.71 Bluegill (Lepomis macrochirus) 8.99 - Fathead minnow (Pimephales 119.81 promelas) 26.84 - Daohnid (Daohnia maana) "3 13 ------- Generating an SSD: 1. Under the SSD module, select either Aquatic or Wildlife. 2. On the SSD taxa selection page, select your surrogate species from the drop down menu and click Add to add the species as a surrogate. 3. If desired, select additional surrogate species from the drop down menu and click Add. A maximum of 25 species can be selected. 4. To remove a surrogate species from the list after it is added, click Remove next to the species name. 5. Enter the known toxicity for the surrogate species, click Calculate SSD. 6. On the SSD output page, the HC/HD level may be changed from drop down box. The hazard level is automatically recalculated if the level is changed. The default is the HC/HD5. 7. The warning "Input toxicity is greater (less) than model maximum (minimum)" indicates if a predicted value was generated from a surrogate species toxicity value that was outside the range of toxicity values used to generate that model. 8. The user can unmark the box to the left of a predicted species to exclude it from the SSD, which is automatically recalculated. (NOTE: See Selecting Predicted Toxicity Values for SSDs in the Guidance for Model Selection and Use section below for guidance on removing estimated toxicity values). 9. The drop down menu in the Show Data column provides additional model information (surrogate, taxonomic distance, cross-validation success rate, degrees of freedom, R2, p-value, or mean square error) for the user to view. 10. The user may sort the ICE-estimated toxicity values by each column by selecting the sort tab below the column heading. III. The Endangered Species Module The Endangered Species Module batch processes toxicity values for endangered species from all species, genus, and family level models available for the entered surrogates. The list of threatened and endangered species was obtained from the US Fish and Wildlife Service Threatened and Endangered Species module of Environmental Conservation Online System (http://ecos.fws.gov/tess_public; Accessed August 2007), which was linked to Web-ICE species, genus, and family model databases for aquatic organisms and wildlife. Users may predict to all available endangered species within a broad taxonomic groups (e.g. Fishes) or a particular species (e.g. Atlantic Salmon, Salmo saia/) using up to 25 surrogates. Producing an Endangered Species Toxicity Report 1. Under the Endangered Species module, select either Aquatic or Wildlife. 14 ------- 2. On the Endangered Species taxa selection page, select either the broad taxa of interest (e.g. Fishes) or a particular species of interest from the drop down menu (Figure 6). 3. Select your surrogate species from the drop down menu and click Add to add the species as a surrogate. A maximum of 25 species can be selected. 4. To remove a surrogate species from the list after it is added, click Remove next to the species name. 5. Enter the known toxicity for the surrogate species, click Calculate. 6. The Endangered species output page provides the estimated toxicity for each predicted taxa, the model level (e.g. species), surrogate, and model information (Figure 7). 7. The user may sort the ICE-estimated toxicity values by each column by selecting the sort tab below the column heading. Interspecies Correlation Estimation HContact us Share You are here: EPA Home » Exposure Assessment » Food Chain » WeblCE » Endangered Species »Aquatic Species Exposure Assessment Models Web-ICE Home Aquatic Species Aquatic Genus Aquatic Family Wildlife Species Wildlife Family Species Sensitivity Distributions Endangered Species Basic Information Download Model Data Bibliography Endangered Species Module - Aquatic Species Step 1: select Taxa of Interest f All Species Fishes f Amphibians C' Crustaceans Molluscs Species: I 3 Sort By: | Common Mame ^ 1 Step 2: select Surrogate(s) Surrogate(s): Sort By | Common Mame ~ | Species Bluegill (Lepomis macrochirus) Fathead minnow (Pimephales promelas) [ Daphnid (Daphnia magna) Toxicity (pg L) Remove Species Remove Species Remove Species Calculate Reset this Form Please address all comments and questions to the webmaster :igure 6. Taxa selection page of Endangered Species module. 15 ------- You are her I ruldn*. Predtctecf Taxa Sort ; Cficorh^richus Ontarrv.tichus Gncofhynchiis Cricorhyrichus SsJ'-ncnidc* ¦¦ielasj, Sli;egi:l (Leporrns macrocfrirtiS). Rainbow trout (Onrorhy?ich"js » Exposure 4-sessnem Foc-d C "/• "• .» >» - " ,-aiic Sweats »Resu!ts Species - Aquatic Surrogate Spec ;ryk)$s-, Sheepshj Input Toxicity: 500:400; 200, 300 yg i Estimated 95% Degrees of R2 p- value Mean Cross- l axonornic Toxicity Confidence Freedom (N- So rr Sort Square Error validation Distance Success m Soil Model Surrogate level Sort Soil Sott so^ces Eli^egill {Lesomis macrochirus) ss>eces Ra-nac.v :rcut 286.82 (Oiicofhynchus ny-ciss? geiL-s Fathead 2~j ' Ba fpimeotiaies orcmetas) Intervals Soft ' 3.27 - 308 87 4 78 99 2) Sort gent-s ¦¦ ¦ "lyiuss.' minnow 243 < rami:,' 1 ..1 1 ¦; . family iamlk' Ra:nbow trout t.elas'- (MSE) Sort 0.9039 O.OC35 0 2064 >.9793 0.9COO 0 0644 5 832S 0-1000 03636 J.S840 O.OCOO 022:5 5 9572 0 0000 0 072: 0 603-2 0 0000 0 : 0.8555 O.OCOO 0 3022 J.SS52 0.0000 0.2 ¦ ? 0 955 5 0 0000 0 0683 Sort 83.33 zigure 7. Endangered species predicted toxicity report IV. Accessing Model Data A list of chemicals in the aquatic and wildlife databases is available for download using the Chemicals in Aquatic and Chemicals in Wildlife links. In the Chemicals in Aquatic file the chemical CAS number and associated toxicity values used in each model are provided. The Chemicals in Wildlife file contains the number of species present for each chemical. The acute data used to develop the ICE models for wildlife are not available due to proprietary rights of some information. Models for all Web-ICE aquatic and wildlife modules are available as a downloadable Microsoft Excel® spreadsheet under the Download Model Data option on the blue navigation bar. The data spreadsheets include model parameters (R2, p-value, df, intercept, slope, standard error of the slope, Sxx, and MSE), general model information (taxonomic distance, cross-validation success rate), descriptive statistics (average, minimum, and maximum values of the surrogate species), and critical t-values used to calculate 90, 95, and 99% confidence intervals (t90, t95, t99). These spreadsheets provide all of the information that is needed to generate Web-ICE toxicity estimates and confidence intervals, as well as facilitate the selection of the most robust models. Using model data provided, users may calculate toxicity as: Predicted toxicity = 10A(intercept + slope*Logio(surrogate toxicity)) 16 ------- And confidence intervals as: Lower bound = 10A(log(predicted) - ti.a*V[MSE*(1/n + (log(x) - x.ave)A2/Sxx) ]) Upper bound = 10A(log(predicted) + ti_a*V[MSE*(1/n + (log(x) - x.ave)A2/Sxx) ]) Where x is the untransformed value of surrogate toxicity, x.ave is the average value of log-transformed surrogate toxicity values, Sxx is the sum of squared deviations of the surrogate, MSE is the mean square error, and ti_a is the value of the t distribution corresponding to the desired level of confidence (ie. 90%, 95%, 99%). Guidance for Model Selection and Use I. Statistical Definitions Several statistics are provided with each model and may be used to evaluate the accuracy and precision of the estimated value. These statistics are shown to the left of the graph on the calculator page (Figure 3C) and are provided in the spreadsheet of model information available in the Download Model Data option. The following provides a basic interpretation of model statistics to help guide users in model selection: Intercept - The log™ value of the predicted taxon toxicity when the log™ of the surrogate species toxicity is 0. Slope - The regression coefficient, represents the change in log™ value of the predicted taxon toxicity for every change in log™ value of the surrogate species toxicity. Degrees of Freedom (df, N - 2) - Reflects the number of data points used to build the model. Degrees of freedom are related to statistical power; in general, the higher the degrees of freedom, the more robust the model. R2 - The proportion of the data variability that is explained by the model. The greater the R2 value and the closer it is to one, the more robust the model is in describing the relationship between the predicted and surrogate taxa. p-value - The significance level of the linear association and the probability that the linear association was a result of random data. Models with lower p-values are more robust. Model p-values of < 0.00001 are reported as 0.00000. Average value of the surrogate - The average of toxicity values for the surrogate species used in the model. The first number is the actual value and the number in parentheses is the log-transformed value. 17 ------- Minimum value of the surrogate - The lowest toxicity value for the surrogate species used in the model. The first number is the actual value and the number parentheses is the log-transformed value. Maximum value of the surrogate - The largest toxicity value for the surrogate species used in the model. The first number is the actual value and the number parentheses is the log-transformed value. Mean Square Error (MSE) - An unbiased estimator of the variance of the regression line. Sum of Squares (Sxx) - Sum of squared deviations of the surrogate. Cross-validation Success - The percentage of removed data points that were predicted within 5-fold of the actual value. Models with a Cross-validation Success of "na" are those that either had df = 1 or where no significant models were developed when data points were removed. Taxonomic Distance - Describes the taxonomic relationship between the surrogate and predicted taxa. Two taxa within the same genus have taxonomic distance of 1; within the same family = 2; within the same order = 3; within the same class = 4; within the same phylum = 5; within the same kingdom = 6. II. Selecting a Model with Low Uncertainty Rules of Thumb Model attributes, such as taxonomic distance of the predicted and surrogate species, model parameters (listed below) and cross-validation success rate, should be used to select models with low uncertainty. For best estimates, models should be selected that possess the following: 1. Relatively low mean square error (MSE) (<0.22) 2. Close taxonomic distance (< 3) 3. High cross-validation success rate (> 85%) 4. High degrees of freedom ( df > 8, N > 10) 5. High R2 value (> 0.6) 6. Low p-values (< 0.01) 7. Narrow confidence bands on the graph The best estimations generally occur for surrogate and predicted taxa that are within the same genus, family, or order and for models with R2 > 0.6 (Raimondo et al. 2007). In general, models with more degrees of freedom (df) have greater statistical power and choosing a model with df greater than 8 is recommended to reduce model 18 ------- uncertainty. A priori power analysis determined that linear models with df > 8 have enough statistical power (1-I3> > 0.8) to sufficiently increase the chance of finding a significant relationship within the data. It is also recommended to choose models with p- values < 0.01 to further reduce the chance of Type I errors in the toxicity estimations. Cross-validation success rate is a conservative estimate of model uncertainty and should not be interpreted as an exact estimate of model error. Cross-validation removes data from the original model, potentially causing a large change in the model for small datasets. Due to changes in a model (i.e. reduced df, altered slope/intercept) during this validation process, cross-validation success rate should be considered only an estimate of generalization error. Particularly for models built from small datasets, actual error can be expected to be lower than cross-validation error. Surrogate Species Selection: An Example In an example of how to select a suitable model, Raimondo et al. (2007) outlined a selection procedure to find an appropriate surrogate species to estimate the toxicity of a chemical to red-winged blackbird. In the example, toxicity data for the chemical of interest was available for northern bobwhite, mallard, Japanese quail, fulvous whistling duck, common grackle, and house sparrow, making them all potential surrogates. The common grackle and house sparrow have the closest taxonomic distance (2, same family; 3, same order); the other potential surrogates in this example have a taxonomic distance of 4 (same class). Of the grackle and house sparrow, both have similar MSE (~0.13), however house sparrow has a higher model R (0.84), higher cross-validation success rate (95), and greater degrees of freedom (107), and is the best surrogate for red-winged blackbird in this example. The grackle would also provide good surrogacy, with high R2 (0.65), high cross-validation success rate (93), and good degrees of freedom (54). If neither of these species were available surrogates, Japanese quail (R2 = 0.79, MSE = 0.15, df = 135, cross-validation success rate = 91) would be the next best surrogate, followed by northern bobwhite (R2 = 0.63, MSE = 0.23, df = 45, cross- validation success rate = 85) and mallard (R2 = 0.48, MSE = 0.34, df = 80, cross- validation success rate = 79). Although fulvous whistling duck has the highest model R2, low degrees of freedom (df = 2) and comparatively higher MSE (0.30) do not make it as suitable of a surrogate as the other species. III. Evaluating Model Predictions Uncertainty of model predictions may be evaluated by assessing (1) the characteristics of the model used in the predictions, and (2) the value of the input data relative to the data used to generate the model. The former was discussed in the previous section and the Rules of Thumb should be followed to ensure high confidence in model selection. Even for robust models, however, model uncertainty increases outside the range of surrogate species toxicity values that were used to develop the model. Uncertainty may be evaluated by reviewing the confidence intervals calculated with the predicted value. Narrow confidence intervals represent higher confidence that 19 ------- the model fits through the range of datapoints for the entered surrogate species toxicity. If the surrogate toxicity value entered into an ICE model is outside the range of surrogate toxicity data used to generate the model, the warning "This value is outside the x-axis range for this model. Continue?" will appear to alert the user. This warning alone does not indicate low confidence in the model estimate, but should be used in conjunction with the calculated confidence intervals to evaluate the model prediction. For example, if the upper and lower bounds of the confidence interval are several orders of magnitude from the predicted value, caution should be used in applying the ICE estimate in risk assessment. IV. Selecting Predicted Toxicity Values for SSDs The SSD modules of Web-ICE automatically predict toxicity values from all available models for the selected surrogate species simultaneously. The user has the discretion to remove predicted toxicity values from the SSD to either customize the SSD for a particular taxa (e.g. birds only, fish only), or to remove predicted toxicity values with large confidence intervals. If an estimated toxicity value was derived from an input value that was outside of the range of surrogate species data used to generate the model from which it was predicted, a warning appears next to the value indicating the maximum or minimum value of the model. This warning alone does not indicate low confidence in the model estimate, but should be used in conjunction with the calculated confidence intervals to evaluate the model prediction. Users should also use the confidence intervals around the HC/HD level to guide the selection of toxicity values to exclude from the SSD. Cases in which the upper bound of the SSD is less than the HC/HD level occur when predicted toxicity values with extremely large confidence intervals are included in the SSD; removal of predicted toxicity with such confidence intervals results in HC/HD values with adequate confidence. Users may also refer to the model information provided by the Show Data dropdown menu when selecting data to include in SSDs. V. Applying Web-ICE in Ecological Risk Assessment fERA) Web-ICE was developed to support both chemical hazard assessment and ecological risk assessment (ERA) by providing a method to estimate acute toxicity to specific taxa, such as endangered species, or to a larger number of taxa (species, genera, family) with known uncertainty. Potential applications of acute toxicity values generated by Web-ICE include the problem formulation phase of an ERA to screen for contaminants of potential concern and in the analysis phase to characterize effects to a larger number of species. The estimation of species-specific toxicity values using Web- ICE is recommended as an alternative to safety factors typically applied when extrapolating toxicity or risks to taxa without chemical and species-specific toxicity data. Another potential application of the chemical and taxon-specific acute toxicity estimates generated from ICE models include input into existing exposure and risk models (e.g. TREX; EPA, 2005). Web-ICE generated toxicity values may also be used in the analysis 20 ------- of uncertainty and variability in toxicity to ecological receptors in both screening level and baseline or Tier II ERAs. In the absence of taxa-specific ICE models, Web-ICE can be used to generate SSDs and estimated 1st, 5th or 10th percentile values of the cumulative distribution of species-specific toxicity values. These percentile values, expressed as the hazard concentration (e.g. HC5) or hazardous dose (e.g. HD5), provide an estimate of toxicity at a prescribed level of species protection with known uncertainty. Hazard concentrations could be used in ERA in place of species-specific toxicity values or as a component of the uncertainty analysis. 21 ------- Acknowledgements For database development, the authors would like to thank Sonny Mayer (US EPA, retired), Thomas Steeger and Brian Montague (US EPA, Office of Pesticide Programs), Don Rodier (US EPA, Office of Pollution Prevention and Toxics), Pierre Mineau, Alain Baril and Brian Collins (National Wildlife Research Centre, Environment Canada), Chris Russom and Teresa Norberg-King (US EPA, Mid-Continent Ecology Division), and Christopher Ingersoll and Ning Wang (Columbia Environmental Research Center, U.S. Geological Survey). Special thanks to Wally Schwab and Derek Lane (Computer Sciences Corporation) for constructing the website, and to Carl Litzinger (US EPA, Gulf Ecology Division) and David Owens (Computer Sciences Corporation) for their facilitation of website development. Also, thanks to our support personnel: Marion Marchetto, Anthony DiGirolamo, Brandon Jarvis, Christel Chancy, Nathan Lemoine, Nicole Allard, Laura Dobbins, Cheryl McGill, Sarah Kell, and Crystal Jackson. Peer review and beta testing of the website were contributed by Larry Goodman, Michael Murrell, Raymond Wilhour, and Susan Yee (US EPA, Gulf Ecology Division), Rick Bennet (US EPA, Mid-Continent Ecology Division), Glen Thursby (US EPA, Atlantic Ecology Division), and Anne Fairbrother (US EPA, Western Ecology Division). References American Society for Testing and Materials (ASTM). 2007. Standard guide for conducting acute toxicity tests with fishes, macroinvertebrates, and amphibians. E 729-96(2007). Philadelphia PA.. Asfaw, A., M. R. Ellersieck, and F. L. Mayer. 2003. Interspecies Correlation Estimations (ICE) for acute toxicity to aquatic organisms and wildlife. II. User Manual and Software. EPA/600/R-03/106. U.S. Environmental Protection Agency, National health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, FL. 14 p. Awkerman, J., S. Raimondo, and M.G. Barron.2008. Development of Species Sensitivity Distributions for wildlife using interspecies toxicity correlation models. Environmental Science and Technology. 42 (9): 3447-3452. Baril, A., B. Jobin, P. Mineau, and B. T. Collins. 1994. A consideration of inter-species variability in the use of the median lethal dose (LD50) in avian risk assessment. Technical Report No. 216. Canada Wildlife Service, Headquarters. De Zwart, D. 2002. Observed regularities in species sensitivity distributions for aquatic species. In Species Sensitivity Distributions in Ecotoxicology, L. Posthuma, G.W. Suter, T.P.Traas, Eds. Lewis Publishers, Boca Raton, FL. pp133-154. Dyer, S. D., D. J. Versteeg, S. E. Belanger, J. G. Chaney, and F. L. Mayer. 2006. Interspecies correlation estimates predict protective environmental concentrations. Environ. Sci. Technol.. 40: 3102-3111. 22 ------- Dyer, S. D., D. J. Versteeg, S. E. Belanger, J. G. Chaney, S. Raimondo and M. G. Barron. 2008. Comparison of Species Sensitivity Distributions Derived from Interspecies Correlation Models to Distributions used to Derive Water Quality Criteria. Environ. Sci. Technol. 42: 3076-3083. Fairbrother, A. 2008. Risk Management Safety Factor. In. Encyclopedia of Ecology, vol. 4. S. E. Jorgensen and B. D. Fath (eds.). Elsevier publishing, pp. 3062-3068. Hudson, R. H., R. K. Tucker, and M. A. Haegele. 1984. Handbook of toxicity of pesticides to wildlife. U.S. Fish and Wildlife Service, Resource Publ. 153, Washington D.C. 90 p. Insightful. 2001. S-plus 6 Guide to Statistics. Volume 1. Insightful Corporation, Seattle, WA. Mayer, F. L. 1987. Acute toxicity handbook of chemicals to estuarine organisms. EPA/600/X-97/332. U.S. Environmental Protection Agency, National health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, FL. 274 p. Mayer, F. L. and M. R. Ellersieck. 1986. Manual of acute toxicity: Interpretation and data base for 410 chemicals and 66 species of freshwater animals. US Fish and Wildlife Service Resource Publication 160. Washington DC. 579 p. Mineau, P., A. Baril, B. T. Collins, J. Duffe, G. Joerman, and R. Luttik. 2001. Pesticide acute toxicity reference values for birds. Rev. Environ. Contam. Toxicol 170: 13-74. Raimondo, S., P. Mineau, and M. G.Barron. 2007. Estimation of chemical toxicity in wildlife species using interspecies correlation models. Environ. Sci. Technol. 5888- 5894. Raimondo, S., D.N. Vivian, C. Delos, M.G. Barron. 2008. Protectiveness of Species Sensitivity Distribution Hazard Concentrations for Acute Toxicity Used in Endangered Species Risk Assessment. Environ Toxicol Chem. 27 (12): 2599-2607. Raimondo, S., D.N. Vivian, and M.G. Barron. 2009. Standardizing acute toxicity data for use in ecotoxicological models: influence of test type, life stage, and concentration reporting. Ecotoxicology. 18:918-928. Shafer, E. W. Jr. and W. A. Bowles Jr. 1985. Acute oral toxicity and repellency of 933 chemicals to house and deer mice. Arch. Environ. Contam Toxicol. 14: 111-129. Shafer, E. W. Jr. and W. A. Bowles Jr. 2004. Toxicity, repellency or phototoxicity of 979 chemicals to birds, mammals and plants. Research Report No. 04-01. United States Department of Agriculture, Fort Collins, CO. 118 p. Shafer, E. W. Jr., W. A. Bowles Jr. and J. Hurlbut,. 1983. The acute oral toxicity, repellency and hazard potential of 998 chemicals to one or more species of wild and domestic birds. Arch. Environ. Contam Toxicol. 12: 355-382. Smith, G. J. 1987. Pesticide use and toxicology in relation to wildlife: organophosphorus and carbamate compounds. Resource Publication 170. United States Department of the Interior, Washington, DC. 171 p. US Environmental Protection Agency (EPA). 1986. Ambient water quality criteria for pentachlorophenol. EPA 440/5-86-009. US Environmental Protection Agency (EPA). 1996. Ecological Effects Test Guidelines. OPPTS 850.1075 Fish Acute Toxicity Test, Freshwater and Marine. EPA 712-C-96- 118. Washington DC. US Environmental Protection Agency (EPA). 2005. TREX: Terrestrial Residue Exposure model. Office of Pesticide Programs. U.S. Environmental Protection 23 ------- Agency. http://www.epa.gOv/oppefed1/models/terrestrial/trex_usersguide.htm#content4 US Environmental Protection Agency (EPA). 2006. ECOTOX Ecotoxicology Database. http://cfpub.epa.gov/ecotox. Duluth MN. 24 ------- Appendices Appendix I. Summary of acceptance requirements for data included in ICE models Component Information required Acceptance requirements Test organism Aquatic taxa tested fish, aquatic invertebrates, amphibians species level model: identifiable to genus and species genus or family level model: identifiable to genus or family Life stage1 juvenile only: fish, amphibians, insects, mollusks, decapods all life stages: all other species Salinity requirements identifiable as freshwater (FW) or saltwater (SW; estuarine or marine) organism Test chemical Test chemical identity reported CAS, chemical name or structure confirmed name and CAS Test chemical purity >90% or analytical/reagent grade or equivalent Single compound tested CAS corresponds to single compound or element mixtures excluded except for chemical salts and specific congener mixtures Test conditions Aqueous exposure no sediment, dietary or mixed dose exposures no phototoxicity results Test duration 48 hr: daphnids, midges, mosquitoes 96 hr: all other species Test type static, flow-through or static renewal Temperature'* species specific (+ 3C) Dissolved oxygen3 Test type specific4 Salinity3 <1 ppt: FW species5 l-5ppt: Cyprinodon bovinus >15 ppt: SW species6 25 ------- Component Information required Acceptance requirements pH or hardness (FW only: required for specific chemical normalizations) pH: ammonia, pentachlorophenol (PCP) Hardness: Ag, Cu, Cd, Cr(III), Pb, Ni, Zn Reported toxicity value Acute toxicity endpoint: death (LC50) or immobilization (EC50) 48 hrEC50/LC50: daphnids, midges, mosquitoes 96 hr EC50/LC50: all other invertebrates 96 hrLC50: fish, amphibians Concentration units mass/volume or molar units Toxicity value standardization Concentration units conversion to ug/L Chemical specific normalizations' PCP: pH 6.5 ammonia: pH 8; temperature dependent Ag, Cu, Cd, Cr(III), Pb, Ni, Zn: hardness 50 mg/L Element specific normalization Ag, Al, Cu, Cd, Co, Cr(III), Cr(VI), Hg, NH4, Ni, Pb, Zn 1. if life stage not reported, must be determined through reported age/size 2. only tests of single compounds; included metal and other chemical salts, and specific congener mixtures (e.g., standard Aroclors, toxaphene) 3. meets ASTM or equivalent test guidelines for test species. 4. Test type specific dissolved oxygen saturation. Static: <48 hr 60-100%; >48 hr 40-100%. Static renewal or flow-through: 60-100%. 5. FW: test water source identifiable as freshwater, reported salinity <1 ppt, or test species is a stenohaline freshwater species; only FW salmonid tests. 6. SW: test water identifiable as saltwater, salinity reported to be > 15 ppt, or test species is a stenohaline saltwater species; only SW striped bass tests were included. 7. normalized according to AWQC 26 ------- Appendix II. List of Species in Aquatic Database The following species were used to develop Web-ICE aquatic species, genus, or family- level models. Invertebrates Tricladida Planariidae Dugesia tigrina Platyhelminthes Annelida Aciculata Nereididae Neanthes virens Lumbriculida Lumbriculidae Lumbricu/us variegatus Flatworm Polychaete Polychaete Insecta Diptera Athericidae Atherix variegata Chironomidae Chironomus p/umosus Chironomus tentans Paratanytarsus c/issimi/is Paratanytarsus parthenogeneticus Midge Odonata Coenagrionidae /schnura verticaiis Plecoptera Perlidae Claassenia sabu/osa Pteronarcyidae Pteronarcella badia Pteronarcys californica Short-horned flies Midge Midge Midge Eastern forktail Stonefly Stonefly Stonefly Crustacea Diplostraca Daphniidae Ceriodaphnia dubia Daphnia magna Daphnia pulex Simocepha/us serru/atus Podocopida Cyprididae Amphipoda Crangonyctidae Crang onyx pseudograci/is Cypris subg/obosa Daphnid Daphnid Daphnid Daphnid Ostracod Amphipod 27 ------- Gammaridae Gammarus fasciatus Hyalellidae Decapoda Camba ridae Penaeidae Isopoda Asellidae Mysida Gammarus /acustris Gammarus pseudo/imnaeus Allorchestes compressa Hyalella azteca Orconectes nais Farfantepenaeus duorarum Metapenaeus dobsoni Aseiius aquaticus Caecidotea brevicauda Caecidotea intermedia Mysidae Americamysis bahia Amphipod Amphipod Amphipod Amphipod Amphipod Crayfish Pink shrimp Kadal shrimp Isopod Isopod Isopod Mysid Forcipulatida Asteriidae Echinodermata Asterias forbesi Ostreoida Ostreidae Basommatophora Planorbidae Pianorbeiia trivoivis Mollusca Crassostrea virginica Starfish Eastern oyster Snail Vertebrates Pisces Acipenseriformes Acipenseridae A cipenser bre virostrum Atheriniformes Atherinopsidae Menidia beryl Una Menidia menidia Cypriniformes Catastomidae Catostomus commersonii Xyrauchen texanus Cyprinidae Carassius auratus Cyprinus carpio Erimonax monachus Gila eiegans Notropis meklstocho/as Pimephaies promeias Shortnose sturgeon Inland silverside Atlantic silverside White sucker Razorback sucker Goldfish Common carp Spotfin chub Bonytail chub Cape fear shiner Fathead minnow 28 ------- Ptychocheilus lucius Cyprinodontiformes Cyprinodontidae Cyprinodon bovinus Cyprinodon variegatus Poeciliidae Gambusia affinis Poecilia reticulata Poeciliopsis occidenta/is Esociformes Esocidae Esox lucius Gasterosteiformes Gasterosteidae Gasterosteus acuieatus Mugiliformes M u g i I i d a e Cheion iabrosus Perciformes Centrarchidae Lepomis cyaneiius Lepomis macrochirus Lepomis micro/ophus Micropterus doiomieu Micropterus sa/moides Pomoxis nigromacuiatus Channidae Channa maruiius Colorado pikeminnow Leon springs pupfish Sheepshead minnow Mosquitofish Guppy Gila topminnow Northern pikeminnow Threespine stickleback Thicklip mullet Green sunfish Bluegill Redear sunfish Smallmouth bass Largemouth bass Black crappie Bullseye snakehead Cichlidae Percidae Sparidae Salmoniformes Salmonidae Siluriformes Ictaluridae Oreochromis mossambicus Etheostoma fonticoia Etheostoma iepidum Perca fiavescens Sander vitreus Lagodon rhomboides Oncorhynchus ciarkii Oncorhynchus giiae Oncorhynchus kisutch Oncorhynchus mykiss Oncorhynchus tshawytscha Sa/mo saiar Sa/mo trutta Salvelinus conf/uentus Salvelinus fontinaiis Salvelinus namaycush Ameiurus me/as Icta/urus punctatus Mozambique tilapia Fountain darter Greenthroat darter Yellow perch Walleye Pinfish Cutthroat trout Apache trout Coho salmon Rainbow trout Chinook salmon Atlantic salmon Brown trout Bull trout Brook trout Lake trout Black bullhead Channel catfish 29 ------- Amphibia Anura Bufonidae Bufo boreas Western toad Ranidae Rana sphenocepha/a Southern leopard frog 30 ------- III. List of Species in Wildlife Database The following species were used to develop Web- models. ICE wildlife species or family-level Aves Anseriformes Anatidae Columbiformes Columbidae Anas discors Bluewinged teal Anas domestica Peking duck Anas piatyrhynchos Mallard Anas superciiiosa Pacific black duck Anas sp. Pintail Anas sp. Widgeon Branta canadensis Canada goose Dendrocygna bicoior Fulvous whistling duck Coiumba iivia Rock dove Coiumba oenas Stock dove Coiumbina inca Inca dove Coiumbina passerina Common ground-dove Geopeiia cuneata Diamond dove Geopeiia humeraiis Bar-shouldered dove Leptotiia verreauxi White-fronted dove Streptopeiia risoria Ringed turtledove Streptopeiia senegaiensis Laughing dove Zenaida asiatica White-winged dove Zenaida auricuiata Eared dove Zenaida macroura Mourning dove Aquiia chrysaetos Golden eagle Faico sparverius American kestrel Falconiformes Accipitridae Falconidae Galliformes Odontophoridae Callipepla caiifornica Callipepla gambeiii Co/inus virginianus Phasianidae Aiectoris chukar Aiectoris rufa Centrocercus urophasianus Cotumix japonica Gaiius ga/ius Meieagris gaiiopavo Perdix perdix Phasianus co/chicus Tympanuchus phasianeiius Gruiformes Gruidae Grus canadensis California quail Gambel's quail Northern bobwhite Chukar Red partridge Sage grouse Japanese quail Chicken Turkey Gray partridge Ring-necked pheasant Sharp-tailed grouse Sandhill crane 31 ------- Passeriformes Corvidae Aphelocoma sp. Scrub jay Corcorax melanorhamphos White-winged chough Corvus bennetti Little Crow Corvus brachyrhynchos American crow Corvus co rax Common raven Corvus coronoides Australian raven Corvus frugi/egus Rook Corvus met tori Little raven Cyanocorax yncas Green jay Pica hudsonia Black-billed magpie Pica nuttaiii Yellowbilled magpie Emberizidae Junco hyemalis Darkeyed junco Spizeiia pallida Clay-colored sparrow Volatinia jacarina Blue back grassquit Zonotrichia atricapilla Golden-crowned sparrow Zonotrichia leucophrys White-crowned sparrow Fringillidae Carpodacus mexicanus House finch Serinus sp. Canary Icteridae Age/a ius phoenice us Red-winged blackbird Ageiaius tricolor Tricolored blackbird Euphagus cyanocephalus Brewer's blackbird Molothrus aeneus Bronzed cowbird Molothrus ater Brown-headed cowbird Quisca/us major Boat-tailed grackle Quisca/us quiscuia Common grackle Xanthocephaius xanthocephaius Yellow headed blackbird Passeridae Neochmia temporalis Red-browed firetail Passer domesticus House sparrow Passer luteus Golden sparrow Taeniopygia guttata Zebra finch Ploceidae Eup/ectes orix Red bishop P/oceus cucuiiatus Village weaver Pioceus taeniopterus Northern masked weaver Que tea que tea Red billed quelea Sturnidae Sturnus vulgaris Starling Turdidae Turdus migratorius American robin Psittaciformes Psittacidae Aratinga canicular is Orange fronted conure Aratinga pertinax Brown-throated conure Calyptorhynchus funereus Yellow tailed black cockatoo Melopsittacus unduiatus Budgerigar Myiopsitta monachus Monk parakeet Piatycercus eiegans Crimson rosella Piatycercus eximius Eastern rosella Psephotus haematonotus Red-rumped parrot Strigiformes 32 ------- Strigidae Megascops asio Eastern screech owl Mammalia Artiodactyla Bovidae Capra hi reus Domestic goat Ovis aries Domestic sheep Cervidae Odocoiieus hem/onus Mule deer Carnivora Canidae Can is famiiiaris Dog Canis iatrans Coyote Lagomorpha Leporidae Lepus caiifornicus Blacktailed jackrabbit Oryctoiagus cunicuius Rabbit Rodentia Caviidae Caviars porceiius Guinea pig Echimyidae Myocastor coypus Nutria Muridae Gerbiiius sp. Gerbil Microtus caiifornicus Meadow mouse Microtus pinetorum Pine mouse Microtus sp. Vole Miscrotus pennsyivanicus Meadow vole Mus muscuius Mouse Oryzomys paiustris Rice rat Peromyscus manicuiatus Deer mouse Rattus argentiventer Ricefield rat Rattus exuians Polynesian rat Rattus norvegicus Norway rat Rattus rattus Roof rat Sigmodon hispidus Cotton rat Sciuridae Cynomys iudovicianus Blacktailed prairie dog Spermoph/ius beecheyi California ground squirrel Spermoph/ius lateralis Goldenmantled ground squirrel Spermoph/ius richardsonii Richardsons ground squirrel 33 ------- |