SEPA SrIPro,ection Analytical Methods Approved for Compliance Monitoring under the Surface Water Treatment Coliform Rule Analysis for the following contaminants shall be conducted in accordance with the methods in the following table, or their equivalent as determined by EPA. The methods and monitoring requirements for these contaminants are specified in 40 CFR 141.74(a)(1) and 40 CFR 141.74(a)(2). Additional methods are listed in Appendix A to Subpart C of Part 141. The CFR is the legal reference for approved methods and takes precedent over this table. The table should accurately reflect the analytical methods information published in 40 CFR 141. §141.74(a)(l)- Analytical and monitoring requirements (a)(1) Analytical requirements (1) The time from sample collection to initiation of analysis may not exceed 8 hours. Systems must hold samples below 10 °C during transit. The procedures must be done in accordance with the documents listed at §141.74(a)(1) or one of the alternative methods listed in Appendix A to Subpart C of Part 141. For Standard Methods Online, the year in which each method was approved by the Standard Methods Committee is designated by the last two digits following the hyphen in the method number. The methods listed are the only online versions of the method that maybe used. For vendor methods, the date of the method listed in §141.74(a)(1) or Appendix A to Subpart C of Part 141 is the date/version of the approved method. The methods listed are the only versions that may be used for compliance with this rule. Laboratories should be careful to use only the approved versions of the methods as product packaging inserts may not be the same as the approved versions of the methods. Contaminant Total Coliforms - lactose fermentation methods: Method Organization Reference Title/Source Date Notes 9221 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods 1992 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. Office of Water (MS - 4606 M) EPA 815-B-24-006 January 2024 ------- Page 2 of 25 Method Organization Reference Title/Source Date Notes No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. 9221 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods 1995 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. 9221 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. 9221 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition Standard Methods 2005 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. 9221 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition Standard Methods 2012 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative ------- Page 3 of 25 Method Organization Reference Title/Source Date Notes rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. 9221 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. 9221 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition Standard Methods 2023 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. 9221 A, B, C-99 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 1999 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. ------- Page 4 of 25 Method Organization Reference Title/Source Date Notes 9221 A, B, C-06 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 2006 Lactose broth, as commercially available, may be used in lieu of lauryl tryptose broth, if the system conducts at least 25 parallel tests between lactose broth and lauryl tryptose broth using the water normally tested, and if the findings from this comparison demonstrate that the false-positive rate and the false-negative rate for total coliforms, using lactose broth, is less than 10 percent. Media should cover inverted tubes at least one-half to two- thirds after the sample is added. No requirement exists to run the completed phase on 10 percent of all total coliform-positive confirmed tubes. Total coliforms - membrane filtration methods: Method Organization Reference Title/Source Date Notes 9222 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods 1992 9222 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods 1995 9222 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 9222 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition Standard Methods 2005 9222 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 9222 A, B, C Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition Standard Methods 2023 ------- Page 5 of 25 Method Organization Reference Title/Source Date Notes 9222 A, B, C-97 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 1997 1604 EPA Method 1604: Total Coliforms and Escherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (Ml Medium), September 2002 EPA Method 1604 2002 Verification of colonies is not required. Total coliforms - enzyme substrate methods: Method Organization Reference Title/Source Date Notes 9223 Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods 1992 9223 Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods 1995 9223 Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 9223 Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition Standard Methods 2005 9223 B Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition Standard Methods 2012 ------- Page 6 of 25 Method Organization Reference Title/Source Date Notes 9223 B Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 9223 B Colilert Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition Standard Methods 2023 9223 B-97 Colilert Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 1997 9223 B-04 Colilert Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 2004 Contaminant Fecal coliforms - Fecal Coliform Procedure (following Lactose Fermentation Methods): Method Organization Reference Title/Source Date Notes 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods 1992 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods 1995 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C ------- Page 7 of 25 Method Organization Reference Title/Source Date Notes 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition Standard Methods 2005 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition Standard Methods 2012 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition Standard Methods 2023 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E-99 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 1999 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C 9221 E-06 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 2006 A-l broth may be held up to 7 days in a tightly closed screw cap tube at 4°C Contaminant Fecal coliforms - membrane filtration methods: ------- Page 8 of 25 Method Organization Reference Title/Source Date Notes 9222 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods 1992 9222D Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods 1995 9222 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 9222D Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition Standard Methods 2005 9222 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition Standard Methods 2012 9222 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 9222 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition Standard Methods 2023 9222 D-97 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 1997 ------- Page 9 of 25 Method Organization Reference Title/Source Date Notes 9222 D-06 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 2006 Contaminant Heterotrophic bacteria - culture method: Method Organization Reference Title/Source Date Notes 9215 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods 1992 9215 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods 1995 9215 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 9215 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition Standard Methods 2005 9215 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition Standard Methods 2012 9215 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 ------- Page 10 of 25 Method Organization Reference Title/Source Date Notes 9215 B-00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 2000 9215 B-04 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. Standard Methods 2004 Contaminant Heterotrophic bacteria - enzyme substrate methods: Method Organization Reference Title/Source Date Notes Simplate IDEXX IDEXX SimPlate™ HPCtest method for Heterotrophs in Water, November 2000. IDEXX Laboratories, One IDEXX Drive, Westbrook, ME 04092 2000 Contaminant Turbidity methoc s: Method Organization Reference Title/Source Date Notes 2130 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition Standard Methods 1992 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin 2130 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition Standard Methods 1995 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin ------- Page 11 of 25 Method Organization Reference Title/Source Date Notes 2130 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition Standard Methods 1998 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin 2130 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition Standard Methods 2005 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin 2130 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition Standard Methods 2012 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin 2130 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition Standard Methods 2017 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin 2130 B Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition Standard Methods 2023 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin 180.1 EPA Methods for the Determination of Inorganic Substances in Environmental Samples, EPA/600/R-93/100, August 1993 National Environmental Methods Index 1993 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin Method 2 Great Lakes Instruments Great Lakes Instruments Method 2, Turbidity, November 2,1992 GLI Method 2 1992 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin 10133 Hach Hach FilterTrak Method 10133, Revision 2.0, Determination of Turbidity by Laser Nephelometry, January 2000 Hach FilterTrak Method 10133 2000 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin ------- Page 12 of 25 Method Organization Reference Title/Source Date Notes M5271 Leek Mitchell Mitchell Method M5271, Revision 1.1, Determination of Turbidity by Laser Nephelometry, March 5, 2009 Mitchell M5271, Rev. 1.1 2009 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin M5331 Leek Mitchell Mitchell Method M5331, Revision 1.1, Determination of Turbidity by LED Nephelometry, March 5, 2009 Mitchell M5331, Rev. 1.1 2009 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin AMI Turbiwell Swan Analytische Instrumente AG Continuous Measurement of Turbidity Using a SWAN AMI Turbiwell Turbidimeter, August 2009 SWAN AMI Turbiwell 2009 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin AQ4500 Thermo Scientific Orion Method AQ4500, Revision 1.0, Determination of Turbidity by LED Nephelometry, May 8, 2009 Orion AQ4500, Rev. 1.0 2009 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin M5331 (Rev. 1.2) Leek Mitchell Mitchell Method M5331, Revision 1.2 Determination of Turbidity by LED or Laser Nephelometry, February 2016 Mitchell M5331, Rev. 1.2 2016 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin 10258 (Rev. 1.0) Hach Company Hach Method 10258, Rev. 1.0 Determination of Turbidity by 360° Nephelometry, January 2016 Hach 10258, Rev. 1.0 2016 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin 10258 (Rev. 2.0) Hach Company Hach Method 10258, Rev. 2.0 Determination of Turbidity by 360° Nephelometry, March 2018 Hach 10258, Rev. 2.0 2018 Revised method allows the use of sealed vials prefilled with Hach StablCal™ for quarterly calibration ------- Page 13 of 25 Method Organization Reference Title/Source Date Notes 8195 (Rev. 3.0) Hach Company Hach Method 8195, Rev. 3.0 Determination of Turbidity by Nephelometry, March 2018 Hach 8195, Rev. 3.0 2018 Revised method allows the use of sealed vials prefilled with Hach StablCal™ for quarterly calibration Lovibond PTV 1000 Tintometer, Inc. Continuous Measurement of Drinking Water Turbidity using a Lovibond PTV 1000 White Light LED Turbidimeter, December 2016 Lovibond PTV 1000, Rev. 1.0 2016 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin Lovibond PTV 2000 Tintometer, Inc. Continuous Measurement of Drinking Water Turbidity using a Lovibond PTV 2000 660-nm LED Turbidimeter, December 2016 Lovibond PTV 2000, Rev. 1.0 2016 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin Lovibond PTV 6000 Tintometer, Inc. Continuous Measurement of Drinking Water Turbidity using a Lovibond PTV 6000 Laser Turbidimeter, December 2016 Lovibond PTV 6000, Rev. 1.0 2016 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin Lovibond TB 3500 Tintometer, Inc. Measurement of Drinking Water Turbidity of a Captured Sample using a Lovibond White Light LED Portable Turbidimeter Lovibond TB 3500, Rev. 1.0 2021 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin Lovibond TB 5000 Tintometer, Inc. Measurement of Drinking Water Turbidity of a Captured Sample using a Lovibond 660-nm LED Portable Turbidimeter Lovibond TB 5000, Rev. 1.0 2021 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin Lovibond TB 6000 Tintometer, Inc. Measurement of Drinking Water Turbidity of a Captured Sample using a Lovibond Portable Laser Turbidimeter Lovibond TB 6000, Rev. 1.0 2021 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin ------- Page 14 of 25 Method Organization Reference Title/Source Date Notes Yokogawa Method 820 Yokogawa Electric Corporation Yokogawa Method 820 - Measurement of Turbidity in Drinking Water by Right Angle Scattered Light Turbidity Analyzer Yokogawa Method 820 2022 Styrene divinyl benzene beads (e.g., AMCO-AEPA-1 or equivalent) and stablilized formazin (e.g., Hach StablCal™ or equivalent) are acceptable substitutes for formazin §141.74(a)(2)- Public water systems must measure residual disinfectant concentrations with one of the analytical methods in the following table or one of the alternative methods listed in appendix A to subpart C of this part. If approved by the State, residual disinfectant concentrations for free chlorine and combined chlorine also may be measured by using DPD colorimetric test kits. In addition, States may approve the use of the ITS free chlorine test strip for the determination of free chlorine. Use of the test strips is described in Method D99-003, "Free Chlorine Species (HOCI" and OCI") by Test Strip/' Revision 3.0, November 21, 2003, available from Industrial Test Systems, Inc., 1875 Langston St., Rock Hill, SC 29730. Free and total chlorine residuals may be measured continuously by adapting a specified chlorine residual method for use with a continuous monitoring instrument provided the chemistry, accuracy, and precision remain the same. Free Chlorine Method Organization Reference Title Date EPA Publication Number Source of Method D99-003 Industrial Test Systems, Inc. Free Chlorine Species (HOCI' and OCI ) by Test Strip November 2003 Method D99-003 D1253-03 ASTM International Standard Test Method for Residual Chlorine in Water 2003 ASTM International D1253-08 ASTM International Standard Test Method for Residual Chlorine in Water 2008 ASTM International D1253-14 ASTM International Standard Test Method for Residual Chlorine in Water 2014 ASTM International 334.0 EPA Determination of Residual Chlorine in Drinking Water using an On-line Chlorine Analyzer September 2009 EPA 815-B-09-013 EPA 815-B-09-013 Hach 10260, Rev. 1.0 Hach Company Determination of Chlorinated Oxidants (Free and Total) in Water using Disposable Planar Reagent-Filled Cuvettes and Mesofluidic Channel Colorimetry April 2013 Hach 10260, Rev. 1.0 ------- Page 15 of 25 Method Organization Reference Title Date EPA Publication Number Source of Method Hach 10241, Rev. 1.2 Hach Company Spectrophotometry Measurement of Free Chlorine (CI2) in Finished Drinking Water November 2015 Hach 10241, Rev. 1.2 ChloroSense Palintest Measurement of Free and Total Chlorine in Drinking Water by Palintest ChloroSense August 2009 ChloroSense ChloroSense, Rev. 1.1 Palintest Free and Total Chlorine in Drinking Water by Amperometry using Disposable Sensors February 2020 ChloroSense, Rev. 1.1 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods ------- Page 16 of 25 Method Organization Reference Title Date EPA Publication Number Source of Method 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-CI H Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI H Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods ------- Page 17 of 25 Method Organization Reference Title Date EPA Publication Number Source of Method 4500-CI H Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods 4500-CI H Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods 4500-CI H Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods 4500-CI H Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-CI H Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-CI D-00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2000 Standard Methods 4500-CI F-00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2000 Standard Methods 4500-CI G-00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2000 Standard Methods 4500-CI H-00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2000 Standard Methods ------- Page 18 of 25 Total Chlorine Method Organization Reference Title Date EPA Publication Number Source of Method D1253-03 ASTM International Standard Test Method for Residual Chlorine in Water 2003 ASTM International D1253-08 ASTM International Standard Test Method for Residual Chlorine in Water 2008 ASTM International D1253-14 ASTM International Standard Test Method for Residual Chlorine in Water 2014 ASTM International 334.0 EPA Determination of Residual Chlorine in Drinking Water using an On-line Chlorine Analyzer September 2009 EPA 815-B-09-013 EPA 815-B-09-013 Hach 10260, Rev. 1.0 Hach Company Determination of Chlorinated Oxidants (Free and Total) in Water using Disposable Planar Reagent-Filled Cuvettes and Mesofluidic Channel Colorimetry April 2013 Hach 10260, Rev. 1.0 ChloroSense Palintest Measurement of Free and Total Chlorine in Drinking Water by Palintest ChloroSense August 2009 ChloroSense ChloroSense, Rev. 1.1 Palintest Free and Total Chlorine in Drinking Water by Amperometry using Disposable Sensors February 2020 ChloroSense, Rev. 1.1 127 EPA Determination of Monochloramine Concentration in Drinking Water January 2021 EPA 815-B-21-004 EPA 815-B-21-004 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods ------- Page 19 of 25 Method Organization Reference Title Date EPA Publication Number Source of Method 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-CI D Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-CI E Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI E Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods 4500-CI E Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods 4500-CI E Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods 4500-CI E Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods 4500-CI E Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-CI E Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods ------- Page 20 of 25 Method Organization Reference Title Date EPA Publication Number Source of Method 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-CI F Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-CI G Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-CI 1 Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI 1 Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods ------- Page 21 of 25 Method Organization Reference Title Date EPA Publication Number Source of Method 4500-CI 1 Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods 4500-CI 1 Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods 4500-CI 1 Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods 4500-CI 1 Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-CI 1 Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-CI D-00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2000 Standard Methods 4500-CI E-00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2000 Standard Methods 4500-CI F-00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2000 Standard Methods 4500-CI G-00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2000 Standard Methods ------- Page 22 of 25 Method Organization Reference Title Date EPA Publication Number Source of Method 4500-CI 1-00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2000 Standard Methods Chlorine Dioxide Method Organization Reference Title Date EPA Publication Number Source of Method 327, Rev. 1.1 EPA Determination of Chlorine Dioxide and Chlorite Ion in Drinking Water using Lissamine Green B and Horseradish Peroxidase with Detection by Visible Spectrometry May 2005 EPA-815-R-05-008 EPA-815-R-05-008 ChlordioX Plus Palintest Palintest ChlordioX Plus Method - Chlorine Dioxide and Chlorite in Drinking Water by Amperometry using Disposable Sensors November 2013 ChlordioX Plus ChlordioX Plus, Rev. 1.1 Palintest Chlorine Dioxide and Chlorite in Drinking Water by Amperometry using Disposable Sensors February 2020 ChlordioX Plus, Rev. 1.1 4500-CI02 C Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI02 C Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods 4500-CI02 C Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods 4500-CI02 C Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods 4500-CI02 C Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods ------- Page 23 of 25 Method Organization Reference Title Date EPA Publication Number Source of Method 4500-CI02 c Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-CIO2 c Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-CIO2 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI02 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods 4500-CI02 D Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods 4500-CI02 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-CI02 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods 4500-CI02 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods 4500-CI02 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods 4500-CI02 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods 4500-CI02 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-CI02 E Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-CI02 C- 00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the 2000 Standard Methods ------- Page 24 of 25 Method Organization Reference Title Date EPA Publication Number Source of Method regulations or in Appendix A to Subpart C of Part 141 are approved. 4500-CI02 E- 00 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to Subpart C of Part 141 are approved. 2000 Standard Methods Ozone Method Organization Reference Title Date EPA Publication Number Source of Method 4500-Os B Standard Methods Standard Methods for the Examination of Water and Wastewater, 18th Edition 1992 Standard Methods 4500-Os B Standard Methods Standard Methods for the Examination of Water and Wastewater, 19th Edition 1995 Standard Methods 4500-Os B Standard Methods Standard Methods for the Examination of Water and Wastewater, 20th Edition 1998 Standard Methods 4500-Os B Standard Methods Standard Methods for the Examination of Water and Wastewater, 21st Edition 2005 Standard Methods 4500-Os B Standard Methods Standard Methods for the Examination of Water and Wastewater, 22nd Edition 2012 Standard Methods 4500-Os B Standard Methods Standard Methods for the Examination of Water and Wastewater, 23rd Edition 2017 Standard Methods 4500-Os B Standard Methods Standard Methods for the Examination of Water and Wastewater, 24th Edition 2023 Standard Methods 4500-Os B- 97 Standard Methods Online Online version. Approval year is designated by the last 2 digits. Only online versions cited in the regulations or in Appendix A to 1997 Standard Methods ------- Page 25 of 25 Method Organization Reference Title Date EPA Publication Number Source of Method Subpart C of Part 141 are approved. ------- |