Final Risk Evaluation for C.I. Pigment Violet 29(Anthra[2,1,9-def:6,5,10-d'e'f']diisoquinoline- 1,3,8,10(2H,9H)-tetrone)Systematic Review Supplemental File: Data Quality Evaluation of Environmental Fate and Transport Studies CASRN: 81-33-4


oEPA

United States

Environmental Protection Agency

Office of Chemical Safety and
Pollution Prevention

Final Risk Evaluation for

C.I. Pigment Violet 29
(Anthra[2,l,9-def:6,5,10-d'eT]diisoquinoline-
1,3,8,10(2H,9H)-tetrone)

Systematic Review Supplemental File:

Data Quality Evaluation of Environmental Fate and Transport

Studies

CASRN: 81-33-4

O

o

o

H

O

January 2021

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This document is a compilation of tables for the data extraction and evaluation for C.I. Pigment Violet
29 (CASRN 81-33-4). Each table shows the data point or set or information element that was extracted
and evaluated from a data source in accordance with Appendix D of the Application of Systematic
Review in TSCA Risk Evaluations (U.S. EPA (20.1.8). If the source contains more than one data set or
information element, the review provides an overall confidence score for each data set or information
element that is found in the source. Therefore, it is possible that a source may have more than one
overall quality/confidence score.

Table of Contents

Table 1. EPI Suite™ Modeling Program, U.S. EPA (2012)	

Table 2. Activated Sludge Study for C.I. Pigment Violet 29, BASF (1999b)
Table 3. Biodegradability Study for C.I. Pigment Violet 29, BASF (1999a).

3
6
9

Page 2 of 12

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Table 1. F.PI Suite™ Modeling Program. ' c' f"° 4

Sludj
Reference:

I .S. I.PA l.sliin;ili(in Programs Interlace Sui(eIM lor Microsoft i< Windows. \ 4.11. 2012.

Domain

Metric

Qualitative
Determination
[i.e., High,
Medium,

Low,

Unacceptable,
or Not rated]

Comments

Metric
Score

Metric

Weighting

Factor

Weighted
Score

Test Substance

1. Test

substance

identity

High

The test substance was
identified by chemical
structure.

1

2

2

2. Test

substance

purity

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

Test Design

3. Study
Controls

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

4. Test

Substance

stability

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

Test

Conditions

5. Test Method
Suitability

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

6. Testing
Conditions

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

7. Testing
Consistency

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

8. System
Type and
Design

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

Test

Organisms

9. Test

Organism

Degradation

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

10. Test

Organism

Partitioning

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

Outcome
Assessment

11. Outcome

Assessment

Methodology

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

12. Sampling
Methods

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

Confounding/

Variable

Control

13.

Confounding
Variables

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

14. Outcomes

Not rated

The metric is not

NR

NR

NR

Page 3 of 12

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Sludj
Reference:

I .S. I.PA I'.siinialion Programs Interlace Sui(eIM lor Microsoft i< Windows. \ 4.11. 2012.



Unrelated to
Exposure



applicable to this study
type (SAR).







Data

Presentation
and Analysis

15. Data
Reporting

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

16. Statistical
Methods and
Kinetic
Calculations

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

Other

17.

Verification or
Plausibility of
Results

Not rated

The metric is not
applicable to this study
type (SAR).

NR

NR

NR

18. QSAR
Models

High

The models in EPI
Suite™ have defined
endpoints. Chemical
domain and
performance statistics
for each model are
known, and
unambiguous
algorithms are
available in the EPI
Suite™ documentation
and/or cited references
to establish their
scientific validity.

Many EPI Suite™
models have
correlation coefficients
>0.7, cross-validated
correlation coefficients
>0.5, and standard
error values <0.3;
however, correlation
coefficients (r2, q2) for
the regressions of some
environmental fate
models {i.e., BIO WIN)
are lower, as expected,
compared to
regressions which have
specific experimental
values such as water
solubility or log Kow
(octanol-water partition
coefficient).

1

1

1







Sum of scores:

2

3

3

High

Medium

Low

Overall Score = Sum

of Weighted
Scores/Sum of Metric
Weighting Factors:

1

Overall
Score
(Rounded):

1

Page 4 of 12

-------
Sliulj
Reference:

1 .S. I.PA l.sliin;ili(in Programs Interlace Sui(eIM lor Microsoft u Windows. \ 4.11. 2012.

>1 and <1.7

>1.7 and <2.3

>2.3 and <3





Overall
Quality
Level:

High

Note: The reviewer agreed with this study's overall quality level.

Page 5 of 12

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Table 2. Activated Sludge Study for C.T. Pigment Violet 29. ™ * 0,7 )

Sludj
Reference:

I5ASI-". 1«)«)'). Delei'ininalion oil lie Inhihit ion ol' Oxygen ( onsiimplion In Acli\aled Sludge In
Penlimid I- in llie Acli\aled Sludge Respiration Inhibition Tesl according lo (.I P. I.N 45001
and l('()'J002. Sludj conducled l)\ I5AS1- Akiiengesellschari l.cologv and l'.n\ ironinenlal
Analytics Laboratory ol' Hcologj IMi^O^fr Ludwigshalen (Siud\ Completion Dale: March I1)')')).

iiiro id: 4^31542

Note:

Conducted according to OECD Guideline 209

Domain

Metric

Qualitative
Determination

[Le., High,
Medium, Low,
Unacceptable,
or Not rated]

Comments

Metric
Score

Metric
Weighting
Factor

Weighted
Score

Test

Substance

1. Test

substance

identity

High

CAS, chemical name,
production number and
date, as well as the
specific form of the test
substance are all
reported.

2. Test

substance

purity

High

Purity 98.9% reported
by analysis

Test Design

3. Study
Controls

High

Blank Control was
included and deviation
of blank control was
reported as <15%. A
reference substance was
also included.

4. Test

Substance

stability

High

Homogeneity, storage
conditions, instability
control were all reported
and appeared to be
appropriate for the test
substance.

Test

Conditions

5. Test

Method

Suitability

Medium

Media concentrations
were provided in terms
of nominal
concentrations (1000
mg/L) and were not
measured. The reported
limit of solubility (2800
mg/L) was not
consistent with the limit
of solubility of this
chemical.

6. Testing
Conditions

High

Testing conditions were
recorded and were
suitable for the test
substance

7. Testing
Consistency

High

Three blank controls, the
test substance were
conducted under the
same conditions.

Page 6 of 12

-------
Sludj
Reference:

I5ASI-". I1)1)1). Delei'ininalion of (lie Inhibition of 0\>gcn Consumption In Acli\alcd Sludge In
Pcnlimid 1- in llie Acli\alcd Sludge Respiration Inhihilion lesl according lo (.I P. I.N 45001
and l('() 9002. Sludj conducled In I5AS1- Aklicngcscllschafl Hcolog\ and I'.m ironmenlal
.\nal> 1 ics l.ahoralon of l-'.colog\ l.udwigshafcn (S(ud\ Completion Dale: March I')1)')).

lll-iKO II): 4"73I542

8. System
Type and
Design

Not Rated

This metric is not
applicable as this is not
an equilibrium test

Test

Organisms

9. Test

Organism

Degradation

High

Inoculum source was a
laboratory wastewater
plant treating municipal
and synthetic sewage;
the dry substance
concentration of the
inoculum was reported
as 1 g/L

10. Test

Organism

Partitioning

Not Rated

This metric is not
applicable as this is not a
partitioning study

11. Outcome

Assessment

Methodology

High

The outcome assessment
methodology is
acceptable to determine
the inhibition of oxygen
consumption by
activated sludge.

Outcome
Assessment

12. Sampling
Methods

Medium

Sampling methods were
not specifically
discussed, but the results
of daily analysis of the
test variables were
reported so this is not
expected to impact the
results of the test.

Confounding/

Variable

Control

13.

Confounding
Variables

High

One study group was
used as this was
conducted as a limit test.
No confounding
variables were observed
or reported by the study
authors.

14. Outcomes
Unrelated to
Exposure

High

Deviation of blank
controls were reported to
be <15%, which
demonstrated the health
of the test organism.

Data

Presentation
and Analysis

15. Data
Reporting

High

Study was conducted as
a limit test, so no effects
were observed in the
study group. Study
authors calculated and
reported EC20, EC50,
and EC80 of reference
substance as well as the
control.

Page 7 of 12

-------
Sludj
Reference:

I5ASI-". I1)1)1). Dclerminalion ol'llie liihihilion of Oxtgcn ( oiisiiiiiplion In Acli\aled Sludge In
Penlimid 1- in (lie Acli\aled Sludge Rospii';ilion liihihilion list according lo (.I P. I.N 45001
iind l('() 9002. Sludj conducted In I5AS1- Aklicngcscllschari Hcolng\ and r.n\ imnmcnlal
AnaMics l.ahoralon of l-'.colog\ IMi^O^ft l.udwigsharcn (S(ud\ Completion Dale: March 1')«)«) i.

lll-iKO II): 4"73I542



16. Statistical
Methods and
Kinetic
Calculations

Not Rated

Statistical analysis was
not conducted as no
adverse effects were
reported.

NR

NR

NR



17.

Verification or
Plausibility of
Results

High

Reported values were
within expected range as
defined by the reference
substance, 3,5-
dichlorophenol.

1

1

1

Other

18. QSAR
Models

Not Rated

QSAR models were not
used as part of this
study. *Note that this
metric has been updated,
as it was originally
evaluated for a metric
that is not part of the
data quality criteria for
fate data.

NR

NR

NR







Sum of scores:

16

19

21

High

Medium

Low

Overall Score = Sum of
Weighted Scores/Sum
of Metric Weighting
Factors:

1.105

Overall
Score
(Rounded):

1.1

>1 and <1.7

>1.7 and <2.3

>2.3 and <3





Overall
Quality
Level:

High

Page 8 of 12

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Table 3. Tiiodegrnd ability Study for C.T. Pigment Violet 29. ™ ^ ' *""" )

Sludj
Reference:

I5ASI-". I«W«). Delei'ininalion oil lie liiodegradahililt of Penlimid I- in llie Manomclric
Kespirnmeln lost according lo CI.P. I.N 45001 and IS()*)002. Sludj conducted In I5AS1-
Aklicngcsellschari l-'.colog\ and l-'.n\ ironnienlal \nal>Iics Laboratory ol' Lcolog\ D-fi-7*I5t>
1 .imIwi^shaleii (S(iid\ Completion Dale: .Inl> IWJ). III.RO II): 4"\3I543

Note:

Conducted according to OECD Guideline 30IF

Domain

Metric

Qualitative
Determination
[Le., High,
Medium,
Low,
Unacceptable,
or Not rated]

Comments

Metric
Score

Metric
Weighting
Factor

Weighted
Score

Test

Substance

1. Test

substance

identity

High

CAS, chemical name,
production number, state
and date of production
were all reported

2. Test

substance

purity

High

Purity reported as 98.9%.

Test Design

3. Study
Controls

High

Blank control and
reference substance were
included (Aniline);
deviation and control
chemical were acceptable
according to test validity
criteria of the guideline.

4. Test

Substance

stability

High

Homogeneity, storage
conditions, instability
controls were all reported.

5. Test

Method

Suitability

Medium

Media concentrations
were provided in terms of
nominal concentrations
(100 mg/L) which was far
higher than the limit of
solubility.

Test

Conditions

6. Testing
Conditions

High

Biodegradation values
were measured and
reported (28 days)
according to reporting
recommendations of
Guideline. Test
temperatures throughout
the test were not
explicitly reported, but
the study authors
indicated that the test was
conduicted "at room
temperature".

7. Testing
Consistency

High

Five control samples and
seven test samples were
conducted under the same
condition.

Page 9 of 12

-------
Sludj
Reference:

I5ASI-". I«)«)«). Dclcrminalion oil lie liiodcgradahililt of Pcnlimid I- in llic Manomclric
Respironiellest according lo CI.P. I.N 45001 and ISO')002. Sludj conduclcd In I5AS1-
Aklicngcscllschari l-lcologj and Hn\ ironmcnlal \nal> 1 ics Laboratory of l-'.colog\ I)-(i705(i
l.udwigshafcn (Sludj Completion Dale: .1 ul> I')*)*)). III.KO II): 4"\5I543

8. System
Type and
Design

Not Rated

Not an equilibrium test.

Test

Organisms

9. Test

Organism

Degradation

High

Inoculum source reported
as municipal activated
sludge from laboratory
wastewater treatment
plants fed with municipal
sewage which is
appropriate.

10. Test

Organism

Partitioning

Not Rated

This is not a partitioning
test.

Outcome
Assessment

11. Outcome

Assessment

Methodology

High

The test methodology
addressed the intended
outcome of interest
(biodegradation
according to the
parameters measured).

12. Sampling
Methods

Medium

Sampling methods were
not specifically discussed,
but the results of daily
analysis of the test
variables were reported.

13.

Confounding
Variables

Medium

The result (Measured
BOD) of one blank
sample deviated from
other 6 test samples. The
authors acknowledged
and disregarded this
sample.

Confounding/

Variable

Control

14. Outcomes
Unrelated to
Exposure

Medium

6 out of 7 test samples
showed similar results,
the degradation rate of all
test samples did not show
any inhibition from the
test substance; One blank
sample showed
anomalous results
discussed by the authors.
The viability of organism
was well maintained.

Data

Presentation
and Analysis

15. Data
Reporting

High

The study received a high
rating for this metric

16. Statistical
Methods and
Kinetic
Calculations

Medium

No statistical analyses
were conducted;
however, sufficient data
were provided to conduct

Page 10 of 12

-------
Sludj
Reference:

I5ASI-". I«)«)«). Dclerminalion oil lie liiodegradal>ilil> «il' Por> limid I- in (lie Mannmelric
Respironiellest according lo CLP. I.N 45001 and IS()')002. Sludj conducted In I5AS1-
Aklicngcscllschal'l l-lcologj iind l-'n\ ir«uiiiieiil;il Allah lies Laboratory of Lcolog\ I)-(i705(i
Ludwigshafen (Sludj ( oni plot ion l);i(e: .1 u l> l«),)«)). III.KO II): 4"\5I543







an independent blaliblieal

analysis.







Other

17.

Verification
or Plausibility
of Results

High

Reported values were
within expected range as
defined by reference
substance(s); Aniline.

1

1

1

18. QSAR
Models

Not Rated

QSAR models were not
used as part of this study.

NR

NR

NR







Sum of scores:

20

20

25

High

Medium

Low

Overall Score = Sum of
Weighted Scores/Sum of
Metric Weighting
Factors:

1.250

Overall
Score
(Rounded):

1.3

>1 and <1.7

>1.7 and <2.3

>2.3 and <3





Overall
Quality
Level:

High

Page 11 of 12

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References

BASF. (1999a). Determination of the biodegradability of perylimid F in the manometric respirometry
test according to GLP, EN 45001 and ISO 9002. Ludwigshafen: BASF Aktiengesellschaft
Ecology and Environmental Analytics Laboratory of Ecology D-67056.

BASF. (1999b). Determination of the inhibition of oxygen consumption by activated sludge by

perylimid F in the activated sludge respiration inhibition test according to GLP, EN 45001 and
ICO 9002. Ludwigshafen: BASF Aktiengesellschaft Ecology and Environmental Analytics
Laboratory of Ecology D-67056.

U.S. EPA. (2012). Estimation Programs Interface Suite™ for Microsoft® Windows, v 4.1 1 [Computer
Program], Washington, DC. Retrieved from https://www.epa.gov/tsca-screening-tools/epi-
suitetm-estimation-program-interface
U.S. EPA. (2018). Application of systematic review in TSCA. risk evaluations. (740-P1-8001).
Washington, DC: U.S. Environmental Protection Agency, Office of Chemical Safety and
Pollution Prevention, https://www.epa.gov/sites/production/files/2018-
06/documetrts/final application of sr in ts<	df.

Page 12 of 12

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