Exposure to Airborne Particles and Gases
and Apportionment to Major Sources
in the Detroit Area (The Detroit Exposure Aerosol Research Study)

Revised Study Plan after Peer Review
September 9, 2003

Major Authors

Anne Rea, Alan Vette, Ron Williams, Jack Suggs, Linda Sheldon,
Roy Fortmann, and Lance Wallace

Contributors

Richard Baldauf, Shaibal Mukeijee, Gary Norris, David Olson, Don Whitaker, William
McClenny, Janet Burke, Carry Croghan, Lucas Neas, Gina Terrill, Mary Ann Heindorf,
Ann Chevalier, Jill Kearney, Catherine Simon, Craig Fitzner, George Bollweg, Margaret

Sieffert, and Rose Dugandzic

National Exposure Research Laboratory
National Health and Environmental Effects Research Laboratory
US Environmental Protection Agency
Research Triangle Park, NC

This document is a preliminary draft of a proposed research study. It has not been
formally released by the United States Environmental Protection Agency and should not
at this stage be construed to represent Agency policy. It is being circulated for comments
on its technical merit and policy implications.

PLEASE DO NOT CITE OR QUOTE


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TABLE OF CONTENTS

1.0	INTRODUCTION	1

1.1	Background	1

1.2	Recent Literature	5

1.2.1	Studies Linking Exposure to Sources	5

1.2.2	Source Apportionment Studies	6

2.0	STUDY OBJECTIVES AND OVERVIEW	9

2.1	Objectives	9

2.2	Study Overview	11

2.3	Collaborations and Partnerships	12

3.0	SURVEY DESIGN	13

3.1	Overview	13

3.2	Study Area	14

3.2.1	Detroit Selection	14

3.2.2	General Information and Demographics	15

3.2.3	Sources of PM and Air Toxics	17

3.2.4	Meteorology	23

3.2.5	Available Monitoring Data	26

3.3	Sampling the Study Population	28

3.3.1	Identifying and Selecting Study Population	28

3.3.2	Selecting Census Blocks	29

3.3.3	Participant Recruitment	31

3.3.4	Selecting the Central Site Monitor	31

4.0	MEASUREMENT PLAN	32

4.1	Overview	32

4.2	Target Pollutants	34

4.3	Field Measurement Protocol	37

4.3.1	Field Monitoring Daily Timetable	37

4.3.2	Sample Locations	37

4.3.3	Sample Management	38

4.4	Monitoring Methods	39

4.4.1	PM2.5 and PMcoarse	39

4.4.2	Nephelometer (personal DataRam®)	42

4.4.3	Elemental Carbon/Organic Carbon	43

4.4.4	Criteria Gases	44

4.4.5	Nitrate	45

4.4.6	Carbonyls	45

4.4.7	VOCs	46

4.4.8	Air Exchange Rate Measurements	46

4.4.9	Supplemental Central Site Monitoring	47

4.4.10	Supplemental Traffic Counts	48

4.5	Survey Instruments	48

4.6	Pilot Study	49

5.0	STATISTICAL ANALYSIS PLAN	49

5.1	Preliminary analysis of existing monitoring data	50

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5.1.1 Sources of monitoring data	50

5.2	Analysis of study data	50

5.2.1 General Linear Models Analysis	56

5.3	Exposure Modeling	60

5.4	Source Apportionment Modeling	61

5.4.1 Detroit Receptor Modeling	61

6.0 QUALITY ASSURANCE	62

7.0	MANAGEMENT	63

7.1	Schedule	63

8.0 REFERENCES	64

Appendix A: Source-Related Exposure Findings	A-l

Appendix B: Power calculation	A-4

Appendix C: Source Apportionment	A-7

Appendix D: Survey Questionnaires	A-l 1

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1.0 INTRODUCTION

1.1 Background

The goal of EPA's air program is to reduce emissions, which in turn, will improve air
quality, reduce exposure to pollutants that cause health impacts, and improve health (US
EPA OIG Report, 2002). To achieve this goal, the Clean Air Act includes provisions that
address criteria pollutants, such as particulate matter (PM), and hazardous air pollutants
or air toxics. For criteria pollutants, the Agency develops and implements air quality
standards that are protective of health by establishing ambient concentration levels above
which health effects are determined to be unsafe. For air toxics, the Agency is directed to
develop standards that address significant sources of pollutants like major industrial
sources and mobile sources and to address urban air toxics by developing standards for
the smaller area sources. Many of these standards will require a risk assessment, which
involves an understanding of how human exposures are affected by specific sources of air
toxics. Setting a standard that is defensible and protective of human health requires a
comprehensive scientific understanding of exposure to ambient pollutants and the
exposure-response relationship.

Particulate Matter

In July 1997 based on an extensive review of the literature, the EPA Administrator issued
new National Ambient Air Quality Standards (NAAQS) for particulate matter (PM) that
revised the standard for PMi0 and added a standard for PM2.5. The new standards were
developed largely on the basis of the epidemiological studies that found consistent
associations between PM concentrations measured at central site monitors and various
adverse health effects. However, individuals develop an adverse health response to PM
in the air that they breathe, not the air at a central site monitor. It seemed almost counter-
intuitive that monitors representing the widely distributed PM mass within an air shed
could serve as a surrogate for individual human exposures, given the diversity of life-
styles and activities. Thus, understanding personal exposures to ambient PM provides a
critical link between regulatory monitoring and health outcomes. Specifically,
understanding the relationship between PM measured at central site monitors and
residential outdoor, indoor, and personal exposure concentrations to ambient PM is
essential to understanding risks.

In 1997, Congress requested that the National Research Council (NRC) review EPA's
Office of Research and Development's (ORD's) PM research program with the objective
of clearly defining research which would reduce "uncertainties in the scientific evidence
used to guide regulation of airborne particulate matter in the United States." Ten priority
research areas were identified in the first NRC report. Two of the ten highest priority
research activities were directed towards understanding PM exposures. Research Topic
1 focused on understanding how susceptible sub-populations were exposed to ambient
PM mass and how these exposures related to concentrations at the central monitor.
Research Topic 2 extended the research to address potentially toxic components of PM
and the general population as well as susceptible sub-populations.

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The need to link PM measured at central site monitors to personal exposure was
recognized early on by ORD. Understanding the source-to-personal exposure component
of the risk paradigm became a primary focus of the PM Research Program.

The goal of ORD's exposure program is to develop data and models that characterize
and predict human exposure to PM of ambient origin relative to that measured at central
sites. Research was initiated in 1997 with a focus on susceptible sub-populations and PM
mass to address NRC Research Topic 1. Longitudinal PM exposure studies were
conducted to characterize inter-personal and intra-personal variability in exposure to PM
mass, and to describe the relationship between personal exposures and ambient exposure
estimates based on central site monitoring. Results from these studies have verified that
for fine PM mass and sulfate, the central monitoring site should serve as an adequate
surrogate for exposure in community-based epidemiological studies. Differences
between ambient levels and estimates of personal exposure should not change the
conclusions regarding epidemiology-based health outcomes, although the strength of the
association has been shown to vary by location, housing characteristics, and season.

Since individuals are typically exposed to lower levels of ambient PM than would be
predicted by central site monitors, the strength of the impact may be underestimated. In
addition, since the ratio of personal exposure concentrations to central site concentrations
(i.e., the attenuation factor) can change by city and season, a single nationwide PM
standard may provide a different degree of protection for different populations. Recent
studies have not shown significant differences in personal exposure to ambient PM as a
result of disease-state.

As a result of our work in NRC Research Topic 1, databases have been developed to
evaluate exposure relationships for PM mass. EPA's exposure program is now focusing
on understanding exposures and exposure relationships for PM components as specified
under NRC Research Topic 2. In the next ten years, it is anticipated that EPA regulations
will dramatically reduce ambient air concentrations of fine particulate mass and sulfates.
As demonstrated in recently completed exposure studies, these two species are well
behaved, and exposure and health effects can be reasonably predicted from central site
monitoring data. To ensure that our standards protect human health in the future,
however, we must be able to evaluate exposure and health effects for those PM species
that will remain after particulate sulfates are removed. Preliminary data show that
outdoor to indoor correlations are poor for several species including ultrafines, acid
aerosols, and nitrates. Other studies have shown that outdoor concentrations of elemental
carbon and several organic species are not homogenous across air sheds and are
influenced by both mobile and stationary sources. For those species that show only weak
associations between central site concentrations and exposures or are not well distributed
across air sheds, it is unlikely that community-based epidemiological studies can be used
to adequately evaluate health impacts. For these species, data and models will be needed
to develop better exposure surrogates for epidemiological studies and to conduct high
quality exposure and risk assessments.

Understanding the association between central site monitoring and ambient exposure (i.e.
the ambient component of total personal exposure), understanding the non-ambient

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exposure component, and knowing the correlation between ambient and non-ambient
exposure for PM mass, PM species and PM sources will be important in the process of
determining whether to revise existing standards. Currently, a national monitoring
network for PM (NCore) is being developed which includes both integrated and real-time
measurements of PM species. It has been proposed that these data, along with data from
the Speciation Trends Network (STN), can provide a foundation for future
epidemiological studies. In addition, the Office of Management and Budget (OMB) is
suggesting that epidemiological studies should be conducted in 50 cities across the U.S.
using the Aerosol Research and Inhalation Epidemiology Study (ARIES) as a model
where health outcomes for PM species were evaluated using a single community monitor.
Before these data can be used in epidemiological studies, it must be shown that the
central site monitor adequately represents exposure for PM species. In addition, models
and information on exposure factors need to be developed to predict exposure based on
measurements at a central site monitor.

As originally stated, NRC Research Topic 2 was to evaluate exposure relationships for
the causal agents of PM toxicity. Although substantial research has been conducted to
understand the mechanisms of PM toxicity and to identify causal agents, specific toxic
agents have not yet been identified, rather there is evidence that supports health effects
associated with most of the originally hypothesized toxic agents. Concurrently, several
epidemiological studies have shown health effects associated with PM from specific
sources rather than focusing on individual components. In light of these findings,
emphasis is now being placed on understanding exposure and health effects of PM from
specific sources. Source apportionment techniques are being incorporated into exposure
research in order to evaluate the ambient-personal exposure relationship for PM from
various sources as well as for individual PM species. Linking specific sources through
central site concentrations and human exposures to health effects is likely to provide data
that can be applied to regulatory policy more quickly, and can help support identification
of biologically important characteristics and constituents as well.

Air Toxics

With the July 19, 1999 Federal Register publication of the National Air Toxics Program:
Integrated Urban Strategy, the EPA provided a road map for its air toxics program. This
program is designed to characterize, prioritize, and equitably address exposures to air
toxics and their serious impact on the public health and the environment through a
strategic combination of regulatory approaches, voluntary partnerships, ongoing research
and assessments, and education and outreach. The program addresses air toxic emissions
from large and small stationary sources, mobile sources, and indoor air sources as part of
its strategy for reducing risks from exposure to air toxics. In addition, the program
prioritizes its actions and measures progress through the use of assessments conducted at
multiple scales (e.g., national, regional, local).

EPA is currently working to characterize the extent of the air toxics problem. National
Air Toxics Assessments (NATA) are one of the four components in EPA's risk-based
National Air Toxics Program and include all of the exposure and risk assessment
activities. NATA is intended to provide EPA and others with improved characterization

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of air toxics exposures and risks based on emissions data for both stationary and mobile
sources, as well as relative risks from indoor air exposures. The NATA are estimated
using the hazardous air pollutant exposure model (HAPEM) to calculate the expected
inhalation exposures of air toxics for population groups using census data, human activity
patterns, ambient air quality levels, climate data and indoor/outdoor concentration
relationships. Ambient levels of air toxics are predicted in the NATA using the
assessment system for populaiton exposure nationwide model (ASPEN), an air dispersion
model based on the industrial source complex long term model (ISCLT). The ASPEN
model uses emissions data from both point and mobile sources along with meteorological
data to estimate dispersion and atmospheric processes that affect the pollutants
(deposition, reactions, secondary formation, etc.). NATA activities also integrate various
Office of Air Research (OAR) office-specific components such as:

•	Residual risk assessments for regulated stationary sources;

•	Listing and de-listing of hazardous air pollutants;

•	Research in mobile source air toxics assessments

•	Research to support the development of an Indoor Air Strategy as well as the
conduct of indoor air assessments; and,

•	Community-based risk assessments and risk reduction projects.

Unfortunately there are limited monitoring data for assessing the extent of the air toxics
problems. EPA is establishing an ambient air monitoring network. Starting in 2003,
thirteen National Air Toxics Trends Sites (NATTS) will be established, with nine more
being added in 2004. These trends sites will measure several key air toxics pollutants
that have been determined to drive risk in assessments conducted to date. In addition to
the NATTS, the ambient air toxics monitoring program will also include community-
based air toxics monitoring projects designed to characterize air toxic concentrations
across a community and to identify sources of potential concern.

There have been some notable air toxics human exposure studies conducted in the past.
These include the TEAM studies (Wallace et al., 1985) and the RIOPA studies (Naumova
et al., 2003). The efforts above were limited in scope in that they were not linked to the
development of human exposure models. Likewise, they did not attempt to estimate the
contribution of major line, point, and regional pollutant sources upon personal and
residential settings.

The Detroit Exposure and Aerosol Research Study (DEARS) is intended to provide
additional data that can be used to evaluate the extent of the problem. Detroit was
selected based on the presence of major industrial and mobile sources. Homes within the
study will be selected to evaluate the impact of these sources on exposures and to
determine high-end exposure. These data will be used to further evaluate and refine
human exposure models that characterize the magnitude of exposure along its uncertainty
and variability. In addition, the methods developed and applied in this study can be used
as a prototype for other community based air toxic programs.

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1.2 Recent Literature

1.2.1 Studies Linking Exposure to Sources

Recent studies have made progress in relating air pollution to sources and the role
individual sources have had upon estimating potential human exposures. Some of these
studies have gone beyond central site-only measurements to include residential and/ or
personal exposures. A number of the most recent studies have been summarized in detail
in Appendix A and will not be presented here. However, a summary of some of the most
important findings is appropriate and will be presented to provide insight as to the current
state-of-the-science in this area and how the proposed study would relate to past research.

In brief, many of these studies have attempted to understand the role that mobile source
emissions from local automotive traffic have had upon personal and residential
concentrations (Van Vliet et al., 1997, Roorda-Knape et al., 1998, Fischer et al., 2000,
Kingham et al., 2000, and Janssen et al., 2001). PM mass concentrations in addition to
mobile source-related VOCs and gases were typically studied. In a number of instances,
these studies revealed that distance from the roadway itself (proximity) was shown to be
a major contributing factor in establishing a source association. One study indicated that
the source influence on air concentrations decreased rapidly when the residential setting
was more than 50 meters from the roadway. The selection of pollutants to be measured
was also observed to be very important. The strength of the central site-residential,
central site-personal correlations associated with mobile source pollutants was
determined to be highly variable among the metrics in many instances. These latter two
points indicate that even where specific source-derived ambient pollutants exist, their
spatial variability across a metropolitan area might be sufficiently high as to result in a
very high level of uncertainty associated with estimating their concentrations based upon
a single measurement location. This might be especially important if a single central site
monitor was used as a surrogate for true personal exposures for all individuals living in a
large metropolitan area where traffic patterns vary.

In addition to the exposure research described above, a number of studies (Van Vliet et
al., 1997, Laden et al., 2000, and Hoek et al., 2002,) have attempted to determine the role
of sources, including that related to mobile sources, upon epidemiological findings. For
example, Laden et al., (2000) were able to use a specialized factor analysis to resolve the
major components of ambient particulate matter and the impact these sources had upon
mortality in six U.S. cities. They discovered that elements associated with resuspended
soil, motor vehicle emissions, and coal combustion has the strongest assocations with the
observed health effect. Both Van Vliet et al. (1997) and Hoek et al. (2002) found that
select exposure measures related to mobile sources (e.g., black smoke) as well as cohort
proximity to roadways were important variables in associating the incidence of asthma
among the local population.

A recently completed investigation of the PM and air toxics associated with vehicle
interiors, roadways and central sites observed a wide range of variability between
pollutant concentrations (Riediker et al., in press). In-vehicle concentrations were usually

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greater than roadside and central site concentrations for certain mobile source-related air
pollutants, such as VOCs, carbonyls, metals, and carbon monoxide. This variability may
be an important factor for individuals spending extensive periods of time involved in
vehicle travel and any resulting health effects.

While numerous studies have attempted to investigate the role of location between
personal, residential and central site PM measurements, few studies have attempted to
relate non-occupational personal exposures directly to ambient sources across a wide
metropolitan area. Studies attempting the simpler case include the longitudinal human
exposure panel studies recently performed by the U.S. EPA and collaborating university
groups (U.S. EPA, 2002; Liu et al., in press). These studies have shown that the
contribution of ambient-derived PM2.5 mass to one's total personal exposure falls into a
range of-45-60%. While defining the role, magnitude and variability of individual
ambient sources upon total personal exposure was not a primary goal of the cited studies,
additional source apportionment work such as that reported by Hopke et al., (in press) is
being performed where possible. Data from these secondary efforts might be used as an
aid in the final design of this proposed study. In addition to the above cited U.S. studies,
the EXPOLIS study performed throughout Europe (Edwards et al., 2001, Kousa et al.,
2002) also focused primarily upon the basic PM mass concentrations relationships.
Additional European studies having a similar study design have been reported by Van der
Zee et al. (1998), Houthuijs et al. (2001), Ruuskanen et al. (2001), and Hoek et al. (2002).

The literature indicated that no substantial or definitive studies have been performed that
have attempted to determine the role of numerous ambient sources upon total personal
exposure or residential settings. This has undoubtedly been due to the lack of appropriate
source apportionment tools, the means to adequately measure source markers
at the central site, residential and potentially the personal setting, and the need to
integrate such an effort into a multi-component human exposure study.

1.2.2 Source Apportionment Studies

Recent source apportionment studies for PMio and PM2.5 suggest that both mobile and
stationary sources are responsible for substantial contributions to the ambient PM
measured in urban and rural locations of the U.S. (Table 1-1). Many previous studies
have focused on elemental analysis. These results were often readily available from
inexpensive XRF analysis of filter samples. Combined with information gathered from
direct source signatures (such as that obtained from stack monitoring), and/or central site
monitoring, source apportionment using receptor modeling was performed. Particulate
matter resulting from the combustion of leaded gasoline often yielded a unique means of
apportioning this source. Unfortunately, a specific marker for automotive emissions
currently does not exist. It is now recognized that the inclusion of organic components
into the analysis is needed to adequately define the source (Schauer et al., 1996; Schauer
and Cass, 2000). These efforts have typically focused upon the use of particle-phase
organics such as straight-chained hydrocarbons, polynuclear aromatic hydrocarbons,
carboxylic acids, hopanes, and sugars as source markers. In some instances, discrete

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markers such as levoglucosan have been used to uniquely identify the contribution of
sources such as woodsmoke.

More recently, the Multilinear Engine (ME) and Positive Matrix Factorization (PMF)
source apportionment tools have been used (Paatero et al., 1999, Hopke et al., in press).
These approaches use a wide number of exposure variables in assigning source
contributions and can easily incorporate data on elemental, organic concentrations, and a
large number of other exposure variable data into their models. Review of concentrations
in the literature cited in Table 1-1 indicate that while a large number of ambient source
apportionment studies have been performed, few data have been reported involving
residential and especially personal-based measures. Ozkaynak et al. (1996) was the first
to attempt such an apportionment using mostly elemental mass concentrations from
residential and ambient-based measurements collected in the PTEAM study. Even so, a
large concentration of the total PM mass could not be accounted for in the apportionment.
Work proposed in the DEARS would represent a major contribution to the science in that
data from a human exposure population would be integrated with extensive residential
(indoor, outdoor) and central site data. Inputs into the source apportionment modeling
would include central site, residential and potentially even personal concentrations of PM
mass, VOCs, SVOCs, carbonyls, sulfates, elements, carbon and criteria gases. State-of-
the-science source apportionment tools, extensively advanced since the PTEAM study,
should enable a large part of the mass to be attributed to specific sources.

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Table 1-1 Source Apportionment Study Results

Sampling Location

Time Period

Percent Contribution

PM2.5 Cone

Diesel

Gasoline

Total
Mobile
Sources

Road
Dust/Soil

Biomass
Burning

Secondary
Sulfate

Secondary
Nitrate

Misc.
Sources

Pasadena, CA (Schauer, 1996)

1982

18.8

5.7

24.5

12.4

9.6

20.9

7.4

24.1

28.2

Los Angeles, CA (Schauer, 1996)

1982

35.7

6.5

42.2

11.1

5.8

20.3

9.2

18.9

32.5

West Los Angeles, CA (Schauer,
1996)

1982

18.0

5.7

23.7

12.2

11.0

24.1

7.8

23.3

24.5

Rubidooux, CA (Schauer, 1996)

1982

12.8

0.7

13.5

13.1

1.2

13.8

24.7

21.6

42.1

Bakersfield, CA (Schauer, 2000)

1995

9.5

3.5

13.0

1.5

16.9

5.0

29.2

25.8

53.8

Fresno, CA (Schauer, 2000)

1995

9.7

2.5

12.2

1.8

49.5

3.5

25.7

19.3

65.9

Sacramento, CA (Motallebi, 1999)

Winter, 1991-96

—

—

24.5

1.2

18.1

4.5

36.6

—

39.5

Bakersfield, CA (Magliano, 1998)

Winter, 1996

—

—

16

<3

20

7

34

—

52

Fresno, CA (Magliano, 1998)

Winter, 1996

—

—

13

<3

19

5

32

—

63

Philadelphia, PA(Dzubay, 1988)

Summer, 1982

—

—

8.5

4.4

—

81.9

—

4.5

27.0

Camden, NJ (Dzubay, 1988)

Summer, 1982

—

—

9.2

3.2

—

81.3

0.4

5.7

28.3

Clarksboro, NJ (Dzubay, 1988)

Summer, 1982

—

—

5.8

2.7

—

84.6

—

2.7

26.0

Welby, CO (Lawson, 1998)

Winter, 1997

10

28

38

16

5

10

25

6

no data

Brighton, CO (Lawson, 1998)

Winter, 1997

10

26

36

11

2

15

32

4

no data

Reno, NV (Gillies, 2000)

Summer, 1998

—

—

68

14.5

4

11

2

0.6

7.8

Phoenix, AZ (Ramadan, 2000)

Summer, 1995-98

10.9

36.2

47.1

1.8

15.0

—

—

36.0

8.3

Phoenix, AZ (Ramadan, 2000)

Winter, 1995-98

14.5

38.9

53.4

1.1

8.9

—

—

36.8

13.8

Baltimore, MD (Hopke, 2003)

Summer, 1998

-

-

16.8

-

-

53.7

<1%

-

22.0

Steubenville, OH (Laden et al.,
2000)

1979-1988

-

5.0

-

14.0

-

57.3

-

23.7

30.5

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2.0 STUDY OBJECTIVES AND OVERVIEW

2.1 Objectives

The overall goal of NERL's Exposure Research Program is to develop the data and models that
characterize and predict human exposure. The Detroit Exposure Aerosol Research Study
(DEARS) is an important step in this program. The study builds upon the results from previous
longitudinal panel studies with several very important differences. The DEARS will examine
the spatial variability of PM2.5 and its components to determine the suitability of conducting
health outcome studies using a central site monitor in an urban area like Detroit where there are
many point sources. Source apportionment techniques will be used to evaluate the relationship
for PM and air toxics from specific sources. Finally, the study is designed to look at and
quantify the impact of local ambient sources on the relationship between central site monitors
and exposures. Results from this study will be critical in providing exposure data for developing
future standards.

Six objectives have been defined for this study.

(1)	To determine the associations between concentrations measured at central site monitors and
outdoor residential, indoor residential and personal exposures for selected air toxics, PM
constituents, and PM from specific sources.

(2)	To describe the physical and chemical factors that affect the relationship between central site
monitors and outdoor residential and indoor residential concentrations, including those that affect
ambient source impacts.

(3)	To identify the human activity factors that influence personal exposures to selected PM
constituents and air toxics.

(4)	To improve and evaluate models used to characterize and estimate residential concentrations
of and human exposures to selected air toxics, PM constituents, and PM from specific sources.

(5)	To investigate and apply source apportionment models to evaluate the relationships for PM
from specific sources and to determine the contribution of specific ambient sources to residential
concentrations and personal exposures to PM constituents and air toxics.

(6)	To determine the associations between ambient concentrations of criteria gases (O3, NO2,
and S02) and personal exposures for these gases as well as personal exposures to air toxics, PM
constituents, and PM from specific sources.

Achieving the first objective will establish the longitudinal correlations between measurements
performed at an central site monitor and residential outdoor/indoor/personal exposure
concentrations. Results will be used to determine if central site measurements for air toxics and
PM species can be used as exposure surrogates in community-based epidemiological studies. As
part of this objective, we will determine if proximity to mobile or stationary sources has an
impact on these relationships. Several design elements are required to meet this objective.
Concurrent monitoring for all targeted pollutants must be conducted at the central monitoring
site, outdoors and indoors at the residence, and on the person. Samples must be collected over
multiple days in order to evaluate longitudinal correlations. The central monitoring site must
have similar requirements for placement as monitors that would be used for epidemiological

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research (i.e., as the speciation sites in NCore or at Supersites). Finally, residences need to be
selected based on proximity to sources.

Under the second objective, we will evaluate and describe the physical and chemical factors that
determine the impact of various ambient sources on outdoor and indoor residential
concentrations and the spatial variability of these concentrations in relation to the source
locations. Results will be used to improve the algorithms in exposure models and to develop
input data for these models. The study design must be directed toward collecting data at central
site, outdoor residential and indoor locations that will describe the most important factors. For
outdoor residential concentrations, this will include location of the residence in relation to the
central site monitor, location of the residence in relation to mobile or stationary sources,
composition and strength of source emissions, and meteorology. For indoor residential
concentrations this includes collecting data on indoor and outdoor concentrations, air exchange
rates, housing characteristics, HVAC operations, and indoor source use. These data when
collected over multiple days will be used to calculate infiltration factors, penetration rates and
removal rates for different chemicals and different housing conditions. Monitoring will be
conducted during both a winter and a summer season for each participant to evaluate the impact
of climate on various factors.

Under the third objective, we will identify factors that influence personal exposures to selected
PM constituents and air toxics using the personal monitoring data and detailed time-activity
information. The impact of personal activities and the time spent in various locations
(residential, non-residential, in vehicles) on personal exposures will be evaluated. Real time
measurements for PM2.5 will be made using personal nephelometers. These data will be used to
determine the impact of spending time in nonresidential locations and personal activities on
exposure. This will be critical in understanding the importance of commuting and work place
activities on exposures.

For the fourth objective, the measurement data and results of the data analysis will be used to
improve the inputs and algorithms used in exposure models. The residential indoor and outdoor
measurement data collected during the study, and the important factors identified by the data
analysis conducted for the second objective, will provide critical information for improving the
inputs and algorithms used in population exposure models for PM constituents and air toxics. A
variety of physically- based mechanistic and stochastic modeling tools will be applied in order to
quantify predicted impacts of major pollutant source categories on outdoor and indoor
concentrations, and personal exposures to PM2.5, PM constituents (e.g., SVOCs, EC/OC) and air
toxics (e.g., VOCs) in the Detroit area. The principal modeling tools that will be chosen for this
specific application, are: the MENTOR (Modeling Environment for Total Risk Studies) system,
and the SHEDS (Stochastic Human Exposure and Dose Simulation) model. The
MENTOR/SHEDS modeling system will be modified and applied to the Detroit study area. The
primary objective of the MENTOR/SHEDS applications will be to test the modeling tools
developed using the DEARS monitoring data in order to evaluate the performance of the model
and perform appropriate model refinements. A secondary objective of these model applications
will be to estimate source-specific contributions of PM and air toxics emissions to outdoor and
indoor concentrations and personal exposures in the Detroit area.

10


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Under the fifth objective, we will refine and apply source apportionment techniques to
understand exposure relationships for PM and air toxics from specific ambient sources. This has
been included as a separate objective since it requires state-of-the-art monitoring for marker
compounds. In addition, the extensive use of source apportionment techniques in personal
exposure studies is a new and challenging application. To meet this objective, source
apportionment models will be used to estimate PM concentrations from specific sources in
central site, residential outdoor and residential indoor samples. These results will then be used to
evaluate and quantify the relationship between central site monitors and residential indoor and
outdoor concentrations for PM from specific sources. Monitoring requirements to meet this
objective are large. Source apportionment models require concentration data on a large number
of marker compounds including elements, elemental carbon, criteria gases, carbonyls, and
semivolatile organic compounds (SVOCs). Concentrations of marker species will be measured
in central site and residential samples. The feasibility of collecting these data in personal
exposure samples and thus extending source apportion methods to personal exposure will also be
evaluated. Source profiles that accurately represent the sources in the study area would greatly
enhance the models. Although not in the current design, opportunities to generate source data
are being investigated.

The sixth objective is intended to determine if central site concentrations of gaseous co-
pollutants (03, N02 and S02) are surrogates or confounders of exposures to air toxics, PM
constituents and PM from specific sources in epidemiological studies. Previous studies showed
that the gaseous co-pollutants acted as surrogates of personal exposures to PM2.5 and sulfate
(Sarnat et al. 2001). Statistical analyses similar to that used by Sarnat et al. will be used to
determine if similar relationships exist between exposures to the gaseous co-pollutants and air
toxics, PM constituents and PM from specific sources.

2.2 Study Overview

The proposed study is a three-year field monitoring study that will be conducted in Detroit,
Michigan and is designed to measure exposure and describe exposure relationships for air toxics,
PM components, PM from specific sources, and criteria pollutants. Monitoring will be
conducted at 120 residences over a three-year period. Measurements of air toxics, PM, PM
constituents, and criteria gases will be collected in each home and from one participant in each
home for five days during both a winter and summer season for a total of 1200 household-
person/days of measurements. A combination of both weekday and weekend sampling will be
conducted in order to evaluate expected variations in industrial source emissions, traffic
volumes, and personal activities. Monitoring is anticipated to start in the summer of 2004.

The residence will be the primary unit for selection and monitoring. The sampling approach will
select households within census tracts that are in close proximity to point and/or mobile sources
in addition to those that are further removed from sources. Census tracts will be further
subdivided into census blocks to contain approximately 50 households. A single participant in
each home will be selected for personal monitoring and to provide time/activity information. The
central site monitoring is located at Allen Park and is part of the Speciation Trends Network. To
further investigate the distribution of pollutants across the study area, one residential outdoor

11


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location will be selected each week to serves as a secondary community site. To achieve this, a
dichotomous sampler will be added to the normal residential outdoor monitoring scheme for a
selected home. These data will be used to determine how spatially representative the primary
and secondary community measures (Allen Park and the selected residential outdoor sites) are
relative to all other outdoor locations.

Measurements will include personal, residential indoor, residential outdoor, and central site
monitoring for PM2.5. VOCs and carbonyls. All PM2.5 filters will be analyzed for mass, elemental
carbon (EC), selected elements, and sulfate as sulfur. Since participants cannot carry a large
number of personal monitors, some pollutants will only be measured indoors, outdoors, and at
the central monitoring site. These include PMcoarse, nitrates, selected SVOCs, and EC/OC as
collected on quartz filters. The criteria gases will only be measured in central site and personal
exposure samples. Survey information will be collected on household characteristics, HVAC
operation, local ambient sources, indoor and personal sources, and time activity patterns.

Data analysis will include statistical analysis to specifically address the study objectives. Human
exposure models and source apportionment models will also be applied to the data. These
analyses will be used to complete annual performance measures set forth in the PM and air toxics
multi year plan.

2.3 Collaborations and Partnerships

The goals in this study are the research needs of the NERL's Human Exposure program.
Therefore, the data measurements and resulting modeling efforts target these needs. There are
however, opportunities for the measurements performed in this work to support other research
areas. The EPA's National Health and Environmental Effects Laboratory (NHEERL) currently
has a planned health study involving asthmatic children in the Detroit-Windsor, Canada
metropolitan area in 2005-2006. While the proposed NHEERL study (the Detroit Children's
Health Study) does not involve any direct human exposure measurements and there is no planned
overlap between subjects participating in the two studies, ambient and local PM and air toxics
measurements performed in the DEARS would serve as inputs into the resulting epidemiological
calculations. This would have potential benefits of reducing the uncertainties in exposure
estimates relative to those based on ambient pollutant concentrations.

In addition to the use of the data described above, there is the potential for the NERL to collect
criteria gas pollutant and VOC data at representative schools associated with the Detroit
Children's Health Study. These samples will be collected in accordance with the study design
currently being developed for the Detroit Children's Health Study and the DEARS.

The daily collection of large quantities of size-fractionated PM from the Detroit metropolitan
area is also of interest to NHEERL researchers. Collection of milligram quantities of PM mass
are needed for toxicological studies. These studies would assess the chemical and toxicological
properities of the various PM size fractions as they relate to potential emission sources. There is
also a possibility that a study funded by the Electric Power Research Institute (EPRI) that
follows the outlines of an earlier EPRI study in Atlanta called ARIES may take place in Detroit.
Part of this study involves an exposure study to be carried out by the Harvard School of Public

12


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Health (Petros Koutrakis, PI). This exposure study would be a good complement to our study
since it would consist partly of "scripted activities," possibly including such activities as
commuting during rush hour. This would provide valuable information on a microenvironment
of interest to the Office of Transportation Air Quality (OTAQ) and to exposure modelers. Once
again, central site data collected in the DEARS would be shared with these collaborators to meet
their research data needs and thereby saving valuable research funding (permitting a larger
scripted population size to be incorporated into the EPRI study). The ARJES-type study design
has been highly touted by OMB as an important study in the determination of source-based
influence upon observed human health effects.

Environment Canada and Health Canada are currently considering research efforts to leverage
those proposed in the DEARS. Limited information is available about any potential collaboration
but it is one in which the NERL will thoroughly consider following its development. One of
preliminary consideration might involve the use of an Environment Canada's mobile laboratory
(van) to help determine concentrations of select pollutants in Wayne County neighborhoods
being considered for DEARS subject recruitment. Data would help determine the magnitude of
pollutant concentrations in neighborhoods being considered and sources potentially influencing
these neighborhoods. These vans have the capability of monitoring various PM size fractions,
VOC concentrations and other air toxics. The NERL would propose providing technical
assistance to any proposed Environment Canada and Health Canada personal and residential
field studies conducted across the border in Windsor, Canada. In addition, data from the DEARS
and Canadian studies would be integrated as appropriate to provide the basis for an investigation
of transborder (U.S.-Canadian) issues.

3.0	SURVEY DESIGN

3.1	Overview

The proposed study is a residential and personal exposure field monitoring study. The primary
goal of the study is to evaluate and describe the relationship between air toxics and PM
constituents measured at a central site monitor and measurements of residential and personal
concentrations. An emphasis is placed on understanding the impact of local sources (point and
mobile) on outdoor residential concentrations and the impact of housing type and house
operation on indoor concentrations. Personal monitoring will be conducted to determine the
impact of time spent in nonresidential locations and personal activities on exposure. Given this
emphasis, the residence will be the primary unit for selection and monitoring. A sampling
approach will be taken to select households within census tracts, in close proximity to point
and/or mobile sources and those that are further removed from sources. Census tracts will be
further subdivided into census blocks to contain approximately 50 households. Personal
exposure data and time activity information will be collected on one participant in each home. A
single central site monitor will be used throughout the study to measure concentrations of PM
constituents and air toxics similar to those measured at the residential and personal level. The
central monitoring location will be selected to mimic community-based monitors that are used in
epidemiological studies. Ideally, the central site monitor should be representative of the airshed.

13


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This section provides general information on the study area. Details are then provided on
selection of housing units, individual participants, and the central site monitor. Finally, the
approach for recruiting study participants is given.

3.2 Study Area
3.2.1 Detroit Selection

Detroit, Michigan was considered the best candidate for this study because of its current and
projected future non-attainment status, the number of point and mobile source influences present,
its geographic location, meteorology, ambient monitoring networks, potential state and local
collaborations, and community-based partnerships. Most importantly, Detroit is currently in non-
attainment status for PM2.5, and is also projected to be in non-attainment status after sulfur
reductions in 2010 (Clear Skies initiative). There are a large number of industrial point sources
of PM and air toxics in the Detroit area, including coke ovens, iron/steel manufacturing, coal-
fired power plants, sewage sludge incineration, automotive industry, refineries, and chemical
plants. The border crossing between Windsor, Canada and Detroit via the Ambassador Bridge
also provides a large diesel and automotive source from idling motor vehicles. There are 4 major
interstates and many heavily traveled roadways, which will serve as line sources of vehicle
emissions in Detroit and surrounding Wayne County. Historical PM and traffic count data are
also available from the area.

The prevailing wind direction (southwest) indicates there should be considerable spatial variation
across the Detroit metropolitan area given the industrial sources located along the eastern edge of
Wayne County. Spatial variation in PM and air toxics concentrations will aid in source
apportionment and modeling. Located in the mid-west, the seasonal fluctuations in temperature
and weather patterns make Detroit a better candidate than a southern city with less of a seasonal
component. There are a number of central monitoring sites currently running in the Detroit area
including a National Air Toxics Trends Site, a Speciation Trends Network site, and a number of
monitoring sites operated by the state of Michigan. The data from these sites will be available
during the study period and we will be able to run additional monitors at an existing site to better
suit our sampling needs.

Recent studies in the area have used a community-based participatory research approach which
has involved the local community in studying 300 school aged children with asthma
(Community Action Against Asthma, CAAA). Working with community partnerships that are
already in place will enhance our ability to recruit and retain participants in a three-year study.
The potential for collaboration with the state of Michigan, Lake Michigan Air Directors
Consortium (LADCO), and local universities also make Detroit a good study location. A number
of other cities were considered for this study, including Houston, TX, Tampa, FL, St. Louis, MO,
and Chicago, IL.

14


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3.2.2 General Information and Demographics

Wayne County is located in southeastern Michigan approximately 60 miles north of the
Michigan/Ohio border (Figure 3-1). It is located on the western edge of Lake St. Clair and
separated from Windsor, Canada by the Detroit River. While the Detroit metropolitan statistical
area (MSA) has a population of 4.4 million and includes six counties (Wayne, Oakland,
Macomb, Monroe, Lapeer, and St. Clair), nearly half (2.1 million) reside in Wayne county alone.

Figure 3-1. Map of southeastern Michigan/northern Ohio.

Wayne County possesses a diversity of ethnicities and housing stock, although their distribution
throughout the County is not homogeneous. Based on data from the 2000 U.S. Census, Wayne
County is approximately 52% White, 42% Black or African-American and 6% other ethnicities.
Hispanics or Latinos comprise only 3.7% of Wayne County. The largest concentrations of
African-Americans and Hispanics in Wayne County are located in Detroit (Figure 3-1). African-
Americans constitute approximately 82% of Detroit's 950,000 people and are present in greatest
numbers in east, west-central and southwest Detroit (>95%). There are other areas of Wayne
County that have a high concentration of African-Americans, namely Inkster located in central
Wayne County as well as portions of southwest Wayne County. Hispanics are primarily located
in southwest Detroit (>40%; Figure 3-1), but only constitute about 5% of Detroit's population.
Whites are primarily located in areas outside of Detroit, namely the entire western portion and
the northeastern tip of Wayne County, in varying degrees (Figure 3-2). The Asian population is

15


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scattered throughout Wayne County with pockets of concentration in Hamtramck, Dearborn and
Plymouth (Figure 3-2).

| united SthodOtWKW
;gr26000sfttrt.WMTE I tgr26000sM tit P0P2000

: 0000000003 ¦ 00*300
| 005001 -02500
102S0I -0?9CO
107401 -09400

1pggn - 8 ago	

Wayne County White Population

Wayne County Asian Population

Figure 3-2. Percentage of Black, Hispanic, White and Asian people by census tract in Wayne
County.

Ftousing stock in Wayne County is also not spatially homogeneous with older residences located
primarily in Detroit and northeastern Wayne County, and newer construction in the remainder of
Wayne County (Figure 3-3). There are, however, isolated census tracts within Detroit and
Dearborn that contain a higher percentage of newer construction. In Wayne County there are a
total of 826,145 housing units of which 73% are detached or attached single-family homes, 16%
are smaller multi-family structures, and 11% are structures with 10 or more housing units. Of all
the housing units in Wayne County 62% are owner occupied while 38% are renter occupied.

16


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Owner occupied units had a median of 6.0 rooms per household and a median household size of
2.74 persons while renter occupied units had 4.4 rooms and 2.43 persons.

Figure 3-3. Distribution of housing stock in Wayne County by census tract.

Home heating fuel in Wayne County is predominately gas, however, owner occupied units rely
more heavily on gas (97%) than do renter occupied units (85%). Electricity follows for home
heating fuel use with owners using it in only 2% of units while renters use electricity for heating
in nearly 13% of units.

3.2.3 Sources of PM and Air Toxics

There are numerous sources of PM and air toxics in the Wayne County airshed. Stationary
sources are located primarily in eastern Wayne County throughout Detroit (Figure 3-4). The
heaviest industry is located in southwest Detroit in and around Zug Island, an industrial complex
on the Detroit River. Industrial sources here include iron/steel manufacturing, coke ovens, oil
refineries, sewage sludge incineration and coal-fired utilities. There are also a number of major
interstates and state highways in Wayne County, which converge most notably in southwest
Detroit (Figure 3-4). This is also the location of the Ambassador Bridge where nearly 10,000
diesel trucks cross each day. Since 9/11, trucks idle at the border crossing for several hours
waiting for inspections and approval to enter/exit the U.S. Still, high traffic volumes are
experienced in western Wayne County as well along two east-west corridors (1-96 to the north
and 1-94 to the south) and one north-south corridor (1-275).

17


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AIRS or Air Topics Sites

#	Allen Park
I Dearborn

~	E. 7 Mile
X Livonia
+ Linwood

~	Fori St. SWHS

•	Wyandotte
A River Rouge
^ Yellow Freight

#	1-696/Lodge

O

Exposure study
monitoring area

Legend

Major Highways

	(-375

Point Sources

At Automotive Industry	[-275

CP Chemical Plants	( ^75

CU Coal Utilities
MW Medical incinerator
MI Municipal incinerator	[.94

OR Oil refinery
MT Metal industries
FS Iron/Steel production

Figure 3-4. Map of Wayne County census tracts showing location of interstates, select industrial
point sources and air toxics monitoring sites. Also shown are areas where study participants will
he selected for exposure monitoring.

3.2.3.1 Stationary Point Sources of PM and Air Toxics

An analysis of stationary source emissions in the Detroit area was conducted to identify the
important sources and source categories for PM and air toxics. There are a number of large PM
and air toxics sources outside of Wayne County in the counties of Monroe, Macomb, St. Clair,
Oakland, and Washtenaw. PM and air toxics emissions from some sources in adjacent counties
may be high enough to impact residential areas in Wayne county, depending on wind direction
and other meteorological factors. Emissions data for the six counties in the Detroit area were
obtained from the National Emissions Inventory database for 1996 (PM and air toxics) and 1999
(PM only). Air toxics data for 1999 have not been finalized in the NEI database at this time, but
are expected to be released during summer 2003. Source emissions data were available by source
within each county, so we were able to identify specific sources with large emissions, as well as
an inventory for the entire county.

The industrial sectors with the highest PM2.5 emissions in the Detroit area are steel production,
power plants, and cement/concrete production. Table 3-1 shows annual PM2.5 emissions for the
largest sources in the Detroit area during 1999 and 1996. The relative emissions of a particular
source or source category varied between years probably due to a combination of economic
conditions and improved control technology. In 1996 the highest PM2.5 emissions were from two
industrial sectors; power plants and steel production. The situation was different in 1999 as PM2.5
emissions from power plants and steel production decreased relative to other sources including
cement/concrete production and glass manufacturing. In 1999, several new casinos were being

18


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built in Detroit, likely resulting in increased production of cement/concrete and glass
manufacturing and consequently higher emissions.

County-wide PM2.5 emissions inventories show that Wayne County emissions were about 2.3
times higher than Monroe county in 1996, but nearly identical in 1999 (Table 3-2). In general,
county-wide emissions varied little between 1996 and 1999 except Oakland County which
increased and Wayne and St. Clair counties which decreased between 1996 and 1999.

Major stationary (point) sources of air toxics in the Detroit area have been identified from the
1996 National Toxics Inventories (http://www.epa.gov/air/data/ntidb.html). Table 3-3 shows
some of the largest point sources of selected air toxics in the Detroit MSA. The air toxics used to
evaluate sources in Detroit were chosen to reflect the types of air toxics that will be measured in
this study and included representative compounds from each of the major categories including
VOCs (benzene and 1,3-butadiene), aldehydes (formaldehyde, acetaldehyde and acrolein),
metals (Ni, Mn, Cr, As, Se and Pb) and PAHs (benzo(a)pyrene and benzo(g,h,i)perylene). For air
toxics, the industrial sectors with the highest emissions in the Detroit area are generally power
plants, iron/steel production and oil refinement, depending on the air toxic. In contrast to PM2.5
emissions, which were highest in Wayne County, emissions of air toxics were generally highest
at facilities in the outlying counties. Exceptions to this are for manganese (iron/steel), nickel (oil
refinery), benzene (steel production and oil refinery) and 1,3-butadiene (steel production). Most
of the area's power plants, which are the largest sources for several air toxics, are located in
counties adjacent to Wayne County. The power plant in Monroe County is the largest source of
many air toxics for the Detroit MSA, but, combined, the Wayne County power plants are
significant sources for several air toxics and are closer to the study population than the Monroe
power plant (about 60 km from Detroit). Similarly, there are significant sources for all of the air
toxics in Wayne County.

19


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Table 3-1. Largest stationary sources of PM2.5 emissions (metric tons) in the Detroit area

Facility

Sector

Location
(County)

PM2.5 Emissions

1999

1996

Pou or

J.R. Whiting

Power

Monroe

251

105

Detroit Edison

Power

Monroe

142

890

Trenton Channel

Power

Wayne

86

205

Detroit Edison - Greenwood

Power

St Clair

84

302

Belle River Power Plant

Power

St. Clair

48

303

River Rouge Power Plant

Power

Wayne

47

157

Steel

National Steel

Steel

Wayne

519

761

Rouge Steel

Steel

Wayne

168

751

DSC LtD.

Steel

Wayne

<27

240

Oil

Marathon Ashland

Oil

Wayne

163

80

Materials

Holnam, Inc.

Cement

Monroe

552

36

Guardian Industries Corp

Glass

Monroe

301

224

Angelos Inc

Concrete

Oakland

279

<26

New Haven Foundry

Metals

Macomb

198

212

Table 3-2. County-wide PM2.5 Emissions Estimates in 1996 and 1999 for Detroit MSA counties
(metric tons).

Coiinlv

I '¦)'¦)'¦)

1

Wayne

1378

2753

Monroe

1211

1187

Oakland

467

103

Macomb

288

302

St. Clair

260

623

Washtenaw

97

84

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Table 3-3. Selected stationary sources for hazardous air pollutants in the Detroit area (pounds).
* Represents the sum of all power plants located in Wayne County (Trenton Channel, River
Rouge, Wyandotte and Mister Sky)

Facility

Sector

Location

Emissions

Amount

National Steel

Steel

Wayne

Benzene
Coke Oven
Manganese

140,863
80,596
10,400

BASF

Chemical

Wayne

Formaldehyde

24,450

Marathon Oil

Oil

Wayne

Nickel

Chromium

Benzene

4,240
1,499
59,065

Detroit Edison

Power

Monroe

Benzene

Formaldehyde

Acetaldehyde

Manganese

Nickel

Chromium

Arsenic

Selenium

Lead

12,175
24,771
5,338
4,592
2,624
2,436
3,842
12,182
3,938

Hancock

Power

Oakland

Formaldehyde

24,584

Peaking Units









Chrysler
Warren Trucks

Automotive

Macomb

Formaldehyde

15,781

Allied Signal

Tar

Wayne

Benzene

17,933

Wayne Co.
Power Plants*

Power

Wayne

Benzene

Formaldehyde

Acetaldehyde

Manganese

Nickel

Chromium

Arsenic

Selenium

Lead

4,584
1,457
2,006
1,766
2,128
927
1,462
4,588
1,501

3.2.3.2 Mobile Sources

In Wayne County, the major highway corridors include Interstates 75, 94, 96, and 275 (Figures
3-4 and 3-5). As an example of traffic flows, Interstate 75 has traffic segments in Wayne County
with 24-hour annual average vehicle counts that range from 63,900 to 195,000 vehicles per day
(Table 3-4). Roadways in Detroit with one-way traffic volume >50,000 vehicles/day are shown
in Figure 3-5.

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Table 3-4. Traffic Volumes on Wayne County Highways

Traffic volumes (24-hour annual averages in vehicles per day);

Wayne County, MI, 2001





Minimum

Maximum



Segment

Segment

Interstate 75

63,900

195,000

Interstate 94

86,200

148,000

Interstate 96

18,600

120,000

Interstate 275

18,100

34,600

U.S. Route 24

14,400

78,400

State Route I

18.800

23,700

State Route 3

14,600

32,700

Figure 3-5. Detroit MSA 2000 census block groups intersecting roadways with one-way daily
flow in excess of 50,000 vehicles.

In addition to these line sources, the major U.S. / Canada border crossings create major point
sources of mobile source emissions as cars and trucks idle at custom stations awaiting inspection.
The major crossings are the Ambassador Bridge (U.S. Interstate 75) and the Detroit/Windsor
Tunnel (U.S. Highway 12). Transit times through U.S. and Canadian customs and inspections
can be lengthy with extended idling times, sometimes for several hours. The high traffic
volumes (Table 3-5) and long transit times will make these two border crossings into significant
sources of mobile emissions, particularly diesel.

22


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Table 3-5. Traffic volumes across U.S. / Canadian Border



Vehicles per day

Location



Cars

Trucks

Latitude

Longitude

Ambassador Bridge

26,000

10,000

42.3117

-83.0742

Detroit/ Windsor Tunnel

28,000

1,000

42.3244

-83.0403

Several studies have measured the contribution of both organic and elemental carbon to the
particulate matter emissions from motor vehicles by operation on chassis dynamometers and
measurements in tunnels. The principal components emitted by diesel and gasoline fueled
vehicles are organic carbon (OC) and elemental carbon (EC). In general, diesel exhaust is higher
in elemental carbon than organic carbon, whereas organic carbon is the dominant species in the
exhaust of gasoline fueled vehicles. Per vehicle, total carbon emissions from light and heavy-
duty diesel vehicles can range from 1 to 2 orders of magnitude higher than those from gasoline
vehicles.

The majority of PM emitted by motor vehicles is in the PM2.5 size range. Particles in diesel
exhaust are typically trimodal consisting of a nuclei mode (<0.1 |j,m), an accumulation mode (0.1
-1.0 |j,m) and a coarse mode (1 - 10 |j,m) that are lognormal in form (Kittelson, 1998). More
than 90% of the total number of particles are in the nuclei mode, which contains approximately 1
to 20% of the particle mass with a mass median diameter of about 0.02 |im, whereas the
accumulation mode (with a mass median diameter of about 0.25 |im) contains most of the mass,
with a smaller fraction (5 to 20%) contained in the coarse mode. Kerminin et al. (1997), Bagley
et al. (1998), and Kleeman et al. (2000) also have shown that gasoline and diesel fueled vehicles
produce particles that are mostly less than 2.0 |im in diameter.

3.2.4 Meteor ol ogy

Meteorological conditions for Detroit during 2002 are summarized in Table 3-6 and depicted in
Figure 3-6. The summary data show that Detroit has long summer and winter seasons punctuated
by shorter fall and spring seasons. Prevailing winds in Detroit are from the southwest during
most times of the year except during March and April when winds are from the northwest. The
predominant southwest winds separate emissions from the source areas in Detroit with the
western part of Wayne County.

23


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Table 3-6 Monthly Summary Statistics for Meteorological Parameters in Detroit during 2002.



Jan.

Feb.

Mar.

Apr.

May

Jun.

Jul.

Aug.

Sep.

Oct.

Nov.

Dec.

Hi Temp

30

33

44

58

70

79

83

81

74

62

48

35

(°F)

























Low Temp

16

18

27

37

47

56

61

60

53

41

32

21

(°F)

























Precip.

2

2

3

3

3

4

3

3

3

2

3

3

(in)

























Snow (in)

9

9

6

1

0

0

0

0

0

0

2

10

Wind Spd.

13

12

13

12

11

10

9

9

9

11

12

12

(mph)

























Wind Dir.

SW

SW

WNW

WNW

SW

SW

SW

SW

SW

SW

SW

SW

24


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512.70

0 0.00

Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec
Record High Snowfall

Month

Figure 3-6. Historic weather patterns in Detroit

25


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3.2.5 Available Monitoring Data

There are two sources of monitoring data in the Wayne County area. The first source is the
AIRS-AQS database and the second is the Detroit Air Toxics Pilot Study (DATPS). The
locations of the monitoring sites within Wayne County are shown in Figure 3-4. Monitoring at
the DATPS sites (I-696/Lodge, Allen Park and Yellow Freight) was operated every 6th day for
one year (April 2001-April 2002). At these sites, TSP metals, VOCs, carbonyls and PAHs were
measured.

Seasonal and annual concentrations of PM2.5 at the air toxics sites in Wayne County were in the
range of 15-20 |ag/m3 during 2002 (Figure 3-7). Levels of PM25 were slightly higher at sites
located in Detroit (Allen Park, SW HS, Dearborn and Wyandotte) than sites in less industrial
parts of Wayne County. PM25 was consistently higher at Dearborn than at any other site in
Wayne County. This is likely due to the close proximity of an automotive production facility
near the Dearborn site.

PM2.5 concentrations were generally similar between seasons except during summer, which were
higher than the rest of the year. At the Dearborn site PM25 concentrations were similar each
season except for winter. The higher PM2 5 concentrations during summer is most likely due to
secondary photochemical formation of aerosols from mobile source related precursors.

AIRS Sites

Figure 3-7. Seasonal and annual PM2 5 concentrations measured at air toxics sites in Wayne
County during 2002.

26


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Composition data of PM2.5 at the Allen Park site show that organic carbon, sulfate and nitrate
constitute about 90% of PM2.5 (Bortnick and Hafner, 2001). Figure 3-8 shows that the
distribution of primary species is variable between season with proportions of nitrate varying
from 11% in summer to 33% in winter. Organic carbon also varies somewhat from 34% in
winter to 44% in fall. Proportions of sulfate remain relatively consistent during each season, but
peaks in summer at 33% of the overall PM2.5 composition.

Winter	Spring

27%

Summer	Fall

Figure 3-8. Seasonal composition of PM2.5 measured at Allen Park.

27


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Preliminary source apportionment of data collected at Allen Park shows several local sources
contribute to the PM2.5 loading at this site (Bortnick and Hafner, 2001). Table 3-7 shows the
regional contribution of PM2 5 at the Allen Park site, the single largest loading factor, was 36%
with the remainder coming from local point and mobile sources. There were discernible
signatures from mobile sources, coal burning, oil and other industrial sources. Although mobile
sources accounted for only 11% (motor vehicle and diesel) of PM2.5 mass directly, there is a
substantial amount related to secondary organic aerosols that are typical of mobile source
emissions.

Table 3-7. Preliminary source apportionment results from Allen Park site.

Factor

Mass
(Hg m3)

% of Total
Mass

Possible Sources

Key Findings

1

1.3

6

Motor vehicle

OC, acetylene, toluene

2

9.6

36

Regional

Almost all NH4 and N03,
50% of S04

3

2.0

9

Coal, smelter

50% S04, many VOCs

4

2.9

14

Oil, industrial

Ni, OC, MEK and
chloromethane

5

4.2

19

VOCs

Aldehydes, MEK, toluene;
higher in summer

6

2.3

11

Industrial

EC/OC -0.9, lower on
weekends

7

1.2

5

Diesel

EC/OC ~3, high Mn, PAH,
Zn, Fe, lower on weekends

3.3 Sampling the Study Population

3.3.1 Identifying and Selecting Study Population

Study participants and households will be identified through a step-wise approach involving
identifying census blocks with and without impacts from local point and/or mobile sources based
on prevailing wind direction and then targeting individual households for recruitment into the
study. Individual census tracts and blocks will be identified by evaluating available data on the
location and emissions from various sources, ambient air concentrations and housing stock. Once
census tracts/blocks are identified we will work through existing community action groups in
Detroit and the surrounding area to assist with contacting individual households. Partnering with
community groups will be important in making connections with residents and communicating
the purpose of the study.

The process by which we will select study participants and households follows.

28


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Select Census Tracts/Blocks

•	Identify the mobile, stationary, and regional sources of PM and air toxics in the Detroit
MSA, with the focus on Wayne County and city of Detroit. Evaluate the size of the
source and potential magnitude of impact.

•	Use available source emissions and air monitoring data to understand the airshed.

•	Identify specific geographical areas and census tracts that may be (1) highly impacted
and (2) those that may be minimally impacted based on their location relative to sources
and meteorology.

•	Identify housing characteristics for the identified census tracts.

•	Evaluate the potential for participation by residents and security in the census tracts.

•	Perform screening measurements in the selected tracts and blocks to assess the potential
impact of sources.

•	Select census blocks within the tracts that best fit these criteria.

Select Housing Unit

•	Develop criteria for inclusion (e.g., non-smoker, non-smoking household, ambulatory,
literate, plans to be in the same dwelling for the next 9 months, detached home, and age
18 or older.

•	Work with community groups to select homes that fit criteria.

Select Study Participants

•	Develop criteria for selection (e.g., age >18 years, working adult, etc.).

•	Select participants from those identified by community organizations.

3.3.2 Selecting Census Blocks

Census blocks will be chosen for monitoring based on proximity to stationary point and mobile
sources and housing stock (age of structure) within the census block. Areas for participant
recruitment and exposure monitoring are shown in Figure 3-4. With predominant southwest
winds and numerous point and mobile source areas located in southwest Detroit, we expect the
highest exposures to occur in northeastern Wayne County including Detroit and Dearborn.
Conversely, we expect the lowest exposures to mobile and point source related PM and air toxics
to occur in southwestern Wayne County. Census blocks will be selected in seven different areas
to characterize personal exposures and residential indoor and outdoor concentrations.

Exposures related to industrial point sources will be measured in two areas of eastern Wayne
County. Census blocks will be selected for monitoring in southwest Detroit near Zug Island (area
1 in Figure 3-4) to characterize exposures from industrial sources (iron/steel and oil refineries).
Exposures to industrial sources will also be characterized in census blocks located near the River
Rouge area of Wayne County (2 in Figure 3-4). Industry in this area is primarily coal-fired
utilities, municipal incinerators and iron/steel. Housing stock in both areas generally consists of
older homes built before 1960. There is a larger percentage of homes built after 1980 in the River
Rouge area, but the majority of homes were built pre-1960.

29


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Mobile source related exposures will be split between areas impacted primarily by diesel and
gasoline powered motor vehicles (i.e., mobile sources). Census blocks will be selected near the
Ambassador Bridge in southwest Detroit to characterize exposures to idling diesel (3 in Figure 3-
4). Diesel exposures will also be characterized in census blocks along Route 53 near E. 7 mile
Rd (4 in Figure 3-4) in Detroit (northeastern Wayne County). This area is more removed from
industrial sources than the Ambassador Bridge and will represent exposures to diesel trucks
driving on a roadway. Houses in these areas were mostly built before 1960. Areas impacted
primarily by gasoline powered motor vehicles in stop-and-go traffic conditions with limited point
source impacts are located in Dearborn (5 in Figure 3-4). Housing stock in this area was mostly
built after 1980. Census blocks will also be chosen near the John C. Lodge Highway to
characterize exposures to stop-and-go traffic (6 in Figure 3-4). Homes in this area were mostly
built before 1960. Areas of southwest Wayne County away from the major interstates are
expected to be minimally impacted by mobile and point sources and would represent upwind
exposures with regional characteristics (7 in Figure 3-4). Housing stock in southwest Wayne
County is generally newer with about half of all homes built after 1980.

The number of households targeted for monitoring in each source category was chosen to
provide sufficient observations of exposures in each category to determine source impacts on
indoor concentrations and personal exposures (Table 3-8). There will be 40 households not
impacted by point sources and 80 households that will be impacted by point sources. Similarly,
45 households will not be impacted by mobile sources, 75 households will be impacted by
mobile sources (45 gasoline, 30 diesel). Approximately 15 households will be in an area that is
expected to be minimally impacted by mobile or point sources. The relatively equal distribution
of homes throughout the study domain should provide sufficient data on exposures to the mix of
sources in Detroit and surrounding Wayne County. The number of homes in each source
category should be adequate to satisfy the objectives and goals of the study.

After individual census blocks are identified as possible monitoring areas, real-time pollutant
data will be collected to assess the impact of mobile, point and regional sources on residential
neighborhoods. Continuous data will be collected on PAH, CO, and PM concentrations at several
locations in the census block. Direct-reading devices will be used which permit fast and efficient
determination of combustion-related pollution gradients. This information will be used to
determine which neighborhoods best match the selection criteria for a given subject population,
such as those that are impacted by gasoline mobile source emissions.

30


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Table 3-8. Proposed matrix of number of households to be selected in each source category. The
number in parentheses corresponds to the area in Wayne County indicated in Figure 3-4.





Mobile Sources









Gas,





Point







stop-

Diesel,

Diesel,

Source





None

and-go

driving

idling

Total



None

15(7)

25 (6)

0

0

40

(L)
O

Coal, oil,

30(1,2)

20 (5)

15(4)

15(3)

80

3
o

GO

steel, power,
incinerator











c
'o

Mobile

45

45

15

15

120

Ph

Source Total











3.3.3	Participant Recruitment

Participant recruitment will be achieved by working with community groups to contact
occupants in residences previously selected from the census blocks. We will work with existing
community level groups such as the Community Action Against Asthma (CAAA) to help
facilitate contact with potential study participants. We will employ an approach that will
minimize selection bias in recruiting. Participants will be selected from the seven geographical
areas that represent various source categories (Figure 3-4, Table 3-8). Households will be
selected on the same side of a given line or point source. Information provided to each family
identified by the community organization as willing to participate will include a description of
the study, inclusion criteria and a brief description of benefits for participating. A detailed
recruitment plan will be developed to select a population that minimizes selection bias. Selection
criteria for participants are: (1) non-smoker, (2) non-smoking household, (3) ambulatory, (4) able
to read and write English, (5) plans to be in the same dwelling for the next 9 months, (6) living in
a detached home, and (7) age 18 or older. Individuals expressing interest in participating in the
study who meet the inclusion criteria will receive a visit from a study recruiter to further explain
the study. At these visits, the study recruiters will obtain informed consent from participants.
Recruitment will continue until the number of needed participants has been obtained. Additional
homes will be selected as needed. Because the study will be performed over a 3-year period,
recruitment and retention of subjects will be performed in the months immediately prior to each
of the monitoring seasons. Retention of subjects participating in two seasons will be performed
by letter and phone call follow-ups. This follow-up will encourage them to remain in the study
and provide them a brief summary of findings to date in their residential area. Approval will be
obtained from all appropriate Institutional Review Boards (IRB) including EPA's Human
Subjects program, prior to contact with potential participants. Members of Research Triangle
Institute, International's IRB will provide the formal review of the recruitment and subject
interaction guidelines.

3.3.4	Selecting the Central Site Monitor

The central site monitor for this study should be an existing monitoring site representative of the
airshed in which the study will take place and one that reflects community based exposures that

31


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are typically used in epidemiological studies. Residential and personal monitoring will be
conducted in areas impacted by point and/or mobile sources and areas with minimal impacts.
Therefore, the central site monitor should be situated such that it captures a variety of exposure
scenarios depending on meteorological conditions. The Allen Park site will serve as the central
monitoring location for this exposure study (Figure 3-4). The Allen Park site is located between
two major interstates (1-94 and 1-75) providing data on mobile source impacts, in close proximity
to but generally not directly downwind of the industrial source region in eastern Wayne County,
and centrally located in Wayne County. Current monitoring capabilities at the Allen Park site
include:

•	Carbon monoxide

•	Ozone

•	PM2 5 and PMio (integrated)

•	pm25 teom

•	Metals

•	PM speciation

•	Meteorology

4.0 MEASUREMENT PLAN

4.1 Overview

Field monitoring will be conducted at 120 residences over a three-year period. Measurements of
air toxics, PM, PM constituents, and criteria gases will be collected in each home and from each
participant for five days during both a winter and summer season for a total of 1200 household-
person/days of measurements. Monitoring is anticipated to start in the summer of 2004. A
summary of the core personal, residential indoor, residential outdoor, and central site monitoring
that will be conducted is given in Table 4-1. A combination of both weekday and weekend
sampling will be conducted in order to evaluate expected variations in industrial source
emissions, traffic volumes, and personal activities.

32


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Table 4-1. Summary of Collection and Analysis Methods

Parameter

Sample

Collection Method

Personal

In Res

Out

Central
Site

Rotating
Community
Site*

PM2.5
Mass
Elements
Sulfate
SVOCs
EC

PM2.5 -
Teflon
filters

PEM (2L)

HI(IOL)

HI(10L)

HI (10L),
PEM (2L)

HI (10L),
PEM (2L)

PMcoarse

Mass,
Elements

PM10 -
Teflon
filters



Sioutas
Cascade
Impactor
(9L)

Sioutas
Cascade
Impactor
(9L)

Sioutas
Cascade
Impactor
(9L)

Sioutas
Cascade
Impactor
(9L)

Fine &
Coarse
PM









Dichot

Dichot

PM2.5

Nephelome
ter

MIE pDR-
1200

MIE pDR-
1200

MIE pDR-
1200

MIE pDR-
1200

MIE pDR-
1200

EC-OC

Quartz
filters
(TOA)



PEM (2L)

PEM (2L)

PEM
(2L)

PEM (2L)

EC

PM2.5
Teflon
filter

PEM (2L)

PEM (10 L)

PEM(IOL)

PEM
(10L)

PEM(IOL)

Nitrate

Mini
denuder
sampler

(glass fiber
filter)



Mini
denuder
(0.8 L)

Mini
denuder
(0.8 L)

Mini
denuder
(0.8 L)

Mini
denuder
(0.8 L)

Ozone

so2
no2

Ogawa
Badge

Passive
badge





Passive
badge

Passive
badge

Carbonyls

DNSH
badge

Passive
badge

Passive
badge

Passive
badge

Passive
badge

Passive
badge

VOCs

Carbopak-
X

Passive
badge or
tube

Passive
badge or
tube

Passive
badge or
tube

Passive
badge or
tube

Passive
badge or
tube

Air
exchange
rate

PFT

—

Passive
collector

—

—



* One residential outdoor location each week will be designated as a temporary community site .
This will only require the additions of a dichotomous sampler and Ogawa criteria gas monitoring
badges to the normal residential outdoor monitoring scheme for this home to be consistent with
the measures collected at Allen Park.

33


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Measurements will include personal, residential indoor, residential outdoor, and central site
monitoring for PM2.5. VOCs and carbonyls. All PM2.5 filters will be analyzed for mass, elemental
carbon (EC), selected elements, and sulfate as sulfur. Since participants cannot carry a large
number of personal monitors, some pollutants will only be measured indoors, outdoors, and at
the central monitoring site. These include PMcoarse, nitrates, SVOCs, and EC/OC as collected on
quartz filters. The criteria gases will only be measured in central site and personal exposure
samples. Carbon will be measured by two methods. Elemental carbon on PM2.5 filter samples
will be estimated based on transmittance of the filter. Using a second approach, elemental and
organic carbon will be collected on quartz fiber filters and then their mass concentration
determined by thermal optical analysis (TOA). This latter method has shown large positive
artifacts for organic carbon in indoor samples; these are speculated to be related to non-ambient
sources resulting from the presence of many potential indoor sources (e.g., carpet, furniture, wall
coverings). Thus, although organic carbon will be measured in indoor samples, data may not be
used unless methods for minimizing the artifact problem can be developed. The indoor samples
will be archived and used in the event these artifact problems can be overcome as well as a
source of samples potentially useful in the analysis of organic aerosol markers. Passive methods
for monitoring VOCs and carbonyls are currently under development. Although these methods
have not been finalized, results to date suggest that performance requirements for this study can
be met.

The central site monitor is located at Allen Park and is part of the Speciation Trends Network.
Monitoring that is ongoing at this site is shown in Section 3.3.4. A dichotomous sampler
(Dichot) will be used to collect 24-hour PM2.5 and PM10-2.5. Filters will be analyzed for mass,
elemental carbon, selected elements and sulfate as sulfur. A prototype dichotomous sampler is
currently being evaluated as an FRM for PM2.5-10 and a FEM for PM2.5. Results from the Dichot
will be used to establish bias between personal and residential monitoring methods and standard
methods. A Sioutas Cascade Impactor will collect PMcoarse. Filters will be analyzed for mass and
selected elements.

A rotating community site will be established at one residence weekly. This will permit for a
direct comparison between community-based measurements across the metropolitan area.
Rotation of the site through each of the seven targeted population areas will permit for a more
extensive evaluation of spatial variability and the representativeness of the Allen Park
community site. Since a base of residential outdoor measurements will already be taking place
for every home incorporated into the study, only the additions of a dichotomous sampler for
PM2.5 and PM10-2.5 mass measurements and and Ogawa badges for criteria gases and a Sioutas
Cascade Impactor for PMcoarSe will permit a consistent sampling scheme as that being performed
at the Allen Park site.

4.2 Target Pollutants

Target pollutants in the following categories will be monitored throughout the study.

•	PM mass - PM2 5, PMcoarse

•	PM species - sulfates, nitrates, elemental carbon (carbon black), organic carbon,
elements, SVOCs

•	Air Toxics -

34


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VOCs - 1,3- butadiene, benzene, carbon tetrachloride, 1,2-
dichloropropane, tetrachloroethylene, trichloroethylene,

Aldehydes -acrolein, acetaldehyde, formaldehyde
Metals -cadmium, lead, manganese, nickel

•	Criteria Gases - NO2, SO2, O3

•	Source Markers - specific markers and their common sources are shown in Table 4-2.

The target compounds were selected based on several criteria. Sulfates, nitrates, and carbon
species make up the bulk of the PM mass for the Detroit area. The VOC and carbonyl air toxics
are those measured at either the National Air Toxics Trends Sites (NATTS) or the Air Toxics
Monitoring Pilot Cities. Currently, acrolein and 1,3 butadiene are not measured at these sites, so
this data may be of use for NATA. The Air Toxic metals are those measured at the same sites
and which are amenable to XRF analysis. The criteria gases have been associated with PM
concentrations and health effects, although the relationship between concentrations of PM and
the criteria gases in exposure samples is unclear. We will measure both to determine if the
criteria gases are confounders or surrogates of PM exposure and the associated health effects.

A number of physical and chemical properties of PM have been hypothesized as causal agents of
PM-induced health effects. Although we have tried to be as inclusive as possible, certain
parameters will not be measured. While it is recognized that carbon monoxide represents an
important pollutant marker for mobile source emissions, the ability to accurately detect this PM-
related pollutant indoors has been shown to be widely problematic. Based upon average ambient
3-4 ppm CO concentrations reported for locations in Wayne County, measurement of this
pollutant inside residences (where concentrations might be expected to routinely fall below 1
ppm — the limit of detection for most portable instrumentation) using portable equipment is not
considered feasible at this time. Therefore, carbon monoxide measurements will not be
performed during the study at any location or on a personal basis. Other pollutant species already
included in the measurement plan are considered adequate for the identification of mobile source
impacts.

Ultrafine PM, acid aerosols and biogenic particles have been excluded because of the high
burden of measuring these in residences and because previous research has shown poor
correlations between indoor and outdoor concentrations. Ultrafines will be collected at the
ambient site. Although metals (V, Cu, Fe, Zn, and Ni) will be measured, soluble metals will not.
We have chosen to measure SVOCs on the filter samples instead. There are no standard methods
for the oxidizing agents such as peroxides, and thus, they are not being measured in this study.
Personal methods for measuring coarse PM are not reliable, and also will not be measured in this
study. However, we will collect PMcoarse inside and outside residences and at the ambient site.
Finally, although certain metals including arsenic, chromium (+6), and mercury are listed as air
toxics, monitoring for these species is beyond the resources for this study.

35


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Table 4-2. Proposed Source Markers

Class

Compound

Source

Elements

Nickel, vanadium

Oil refineries/oil combustion



Manganese

Iron/steel production



Sulfur

Sulfate



Potassium

Wood



Iron

Iron/steel production



Zinc

Incineration



Silicon, Aluminum

Road dust

Carbon

Elemental

Diesel, mobile sources



Organic

Mobile sources

Ions

Sulfate

Regional background, coal burning



Nitrate

Regional background, mobile sources

VOCs

MTBE, benzene, toluene, xylenes, 1,3-
butadiene

mobile sources



Benzene

Steel production, oil refineries, coke ovens



Ethyl benzene

Mobile sources, oil refineries



Toluene

Oil refineries, coke ovens



Xylene

Oil refineries, iron/steel production

Carbonyls

Acrolein

Mobile sources



Formaldehyde

Mobile sources (outdoors), building materials,
furnishings (indoors)



Acetaldehyde

Mobile sources

SVOCs

Norhopane, hopane, homohopane,
bishomohopane

Petroleum/mobile sources



Levoglucosan

Wood smoke

Alkanes

C27-C30

Mobile sources, wood combustion, natural gas
combustion

PAHs

pyrene, chrysene/triphenylenen,
benzo [kjfluoranthene,
benzo [b]fluoranthene,
benzo [a]pyrene,indeno [1,2,3-
cd]pyrene, benzo [ghijperylene

Mobile sources, wood combustion, natural gas
combustion

36


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4.3 Field Measurement Protocol

4.3.1	Field Monitoring Daily Timetable

Field monitoring for each participant will be conducted over a 5-day period during two seasons
within a year. Activities taking place in each home over the 5-day sampling period are shown in
Table 4-3. Sample and data retrieval at each home will take ~ 45 minutes and will occur
between 6 am and 10 am. Ideally, sample changeout for all participants should take place at the
same time as the central site monitor (8 am) to give a direct comparison of the data. However,
this is not practical due to staffing limitations and the need to accommodate the participant's
schedules. Rather, samples will be changed as close as reasonably possible to the central site
sampling time (projected to be ± 2 hours). Two 3-person teams will conduct sample and data
retrieval activities in the participant's homes. Each team will be responsible for monitoring
three homes per day. Thus, six homes/participants will be monitored during a 5-day period.
Monitoring schedules will be staggered for each home/participant so that both weekday and
weekend data collection will occur. Approximately 40 homes/participants will be monitored
over a 10-week period each season. New participants will be recruited each year.

4.3.2	Sample Locations

Samples will be collected at an outdoor and indoor residential site at each home. The outdoor
site will be on the non-roadway side of the home. For mobile source targeted homes that are
surrounded by roads, the outdoor monitor will be located on the least traveled segment/road side
of the home. The site will be located using the best available siting criteria relative to the
conditions present at each residence (i.e. away from the residence or other buildings, any
roadway, parking lots, or other known sources of PM). The indoor residential site will always be
the primary living area in each home, which is defined as the non-bedroom area where an
individual would spend most of their time. Samples will be collected away from heating vents,
known sources of PM, and at least one meter from the wall. Both indoor and outdoor samples
will be collected 1 to 1.5 meters above the ground. For personal exposure samples, pumps will
be secured in the pocket of a lightweight cloth vest or coat; the sampling heads will be attached
to the lapel to collect air from the breathing zone.

37


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Table 4-3. Sample Collection in Each Household

Day

Time

Activity

Pre3

10 am to 4 pm
(length: ~ 30
minutes)

1.	Obtain informed consent

2.	Deploy PFT emitters

3.	Measure house volume for air exchange rate measurement

4.	Administer household questionnaire

5.	Confirm schedule for next monitoring day

1

6 to 9 am
(length: ~ 45
minutes)

1.	Set up indoor/outdoor monitoring equipment

2.	Install sampling filters/cartridges in all equipment

3.	Measure sampling flow rates

4.	Give participant personal monitor in sampling vest

5.	Give detailed instructions for personal monitoring and diary completion.

6.	Set out PFT collectors

7.	Confirm schedule for next monitoring day

2-4

6 to 9 am
(length: ~ 45
minutes)

1.	Measure sampling flow rates in all equipment to end current session

2.	Replace sampling filter/cartridges in all equipment

3.	Replace PFT collectors

4.	Measure sampling flow rates of replacement equipment

5.	Give participant personal monitor in sampling vest

6.	Download nephelometer data

7.	Review daily diary; Administer post monitoring questionnaire

8.	Confirm schedule for next monitoring day

5

6 to 9 am
(length: ~ 45
minutes)

1.	Measure sampling flow rates in all equipment

2.	Remove sampling filter/cartridges; disassemble all equipment

3.	Collect PFT collectors and emitters

4.	Download nephelometer data

5.	Review daily diary; Administer post monitoring questionnaire

6.	Pay incentive

a Will be performed 24 to 48 hours prior to field monitoring

4.3.3 S ampl e Management

All samples (field sample, field duplicate, laboratory blank, field blank, etc.) will be given a
unique bar code label at the time of preparation. Specific identifiers will be used for each set of
samples so that different size fractions, collection instruments, collection dates and other key
identifiers will be easily discernable to avoid operator error during sample collection and
analysis. Data log sheets and an electronic sample collection "shell" will be used in conjunction
with the bar codes to track sample collection (collection schedule, recovery, and transport).

Field notebooks will be used to record sampling information regarding instrument audits, flow
verifications and other factors ensuring the integrity of the data collection. Senior field staff will
be responsible for daily archival of samples, their handling, and transport back to the laboratory.

Collected samples will be stored in the field at < 0°C after their collection. Samples will be
shipped to the laboratory weekly via overnight carrier in chilled containers. Dedicated study

38


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freezers of < -20°C will be used to store samples prior to analysis. Chain of custody forms will
accompany all samples from the time of their collection through storage, analysis, and archiving.
Individuals handling the samples will acknowledge this action by signature and date on the chain
of custody forms that will remain with the samples until the time of their disposal. Copies of this
record will be maintained in the study files at all times.

4.4 Monitoring Methods

4.4.1 PM2 5 and PMcoarse

4.4.1.1	Collection

PM2.5 will be collected using inertial impactor samplers. All of these samples will be collected
for 24 hours onto pre-weighed 37 mm Teflon filters (Teflo®-Gelman Sciences, Ann Arbor,
Michigan). The Harvard Impactor (HI) samplers will be used at the central site, outdoors at the
home, and indoors at the home to collect PM2.5 at flow rates of 10 LPM. The HI particle sampler
uses an oiled porous impactor plate to minimize particle bounce off while also providing a sharp
cut point. The PEM (Personal Environmental Monitor, MSP model 100) PM2.5 sampler operates
at a flow rate of 2 LPM and will be used to collect personal air samples. The PEMs inlets have
been modified with a4(xm "scalper plate" (MSP#PEM-019) to reduce large particle burden upon
the impactor during the sample collection. PEMs sampling pumps have been modified to permit
extended operation as well as temperature, flow rate, and motion sensing data logging. PM2.5
mass concentrations collected on PEM samplers have been shown to be equivalent to those
produced by collocated FRM samplers in earlier NERL panel studies (Williams et al., 2000).

The Sioutas Cascade Impactor (SKC, PA) will be used to collect five size ranges of PM for a
PMCOarSe measurement. The Sioutas Cascade Impactor consists of four impaction stages and a
final filter that allows the separation and collection of airborne particles in five size ranges. It
uses a flow rate of 9 LPM. Particles above each cut-point are collected on a 25-mm PTFE filter
in the appropriate stage. Particles below the 0.25 |j,m cut-point of the last stage are collected on a
37-mm PTFE final filter. Size-fractionated samples can be analyzed gravimetrically, chemically,
and microscopically.

4.4.1.2	Analysis

Mass, EC, elements, and SVOCs collected on the filter samples will be analyzed using
gravimetric, XRF, optical transmission, and GC-MS methods, respectively. The first three
methods are nondestructive thus multiple analyses can be performed. For SVOCs, filters must
be extracted prior to GC/MS analysis. Once this is done no additional analyses can be
performed.

Mass. Gravimetric analysis of PM filter samples will be performed following 24-hour
equilibration at 25°C (± 5°) and 40% relative humidity (± 5%). The method quantitation limit for
the PEM PM monitoring has been determined to be 1.91 |ig/m3 with a collocation precision (root
mean square) of ± 4.3 (j,g/m3. These historical values indicate that the gravimetric method to be
used will be sufficient under even the most stringent conditions (low volume personal

39


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monitoring). Lawless and Rodes (1999) have reported upon the gravimetric procedures required
to accurately measure filter mass loadings from low volume air sampling.

Elemental Carbon. An optical transmittance procedure, currently being evaluated using data
from two recent EPA studies (the RTP Panel Study and the Tampa Children's Study) will be
used to establish EC concentrations. Data from our recent studies is being used to compare
results between this method and the thermal optical analysis (TOA) method to determine bias.

Elements. Multiple elements can be measured using X-ray Fluorescence techniques on samples
collected using Teflon filters. The filter is irradiated with x-rays and then elemental fluorescence
is quantified. The detector is placed at an appropriate angle to measure the intensity at a certain
wavelength. The elements of primary interest and their estimated detection limits are given in
Table 4-4. As the table shows, although samples will be analyzed for all of these elements, some
of them may not be at sufficiently high concentrations to be detected. Precision ranges from 6-9
% depending on the concentration, while accuracy is +/- 10%.

Table 4-4. Elemental detection limits and environmental concentrations (ng/m3).

ng/m3

Detection Limits

Environmental Concentrations

Element

PEM (2LPM)

HI(IOLPM)

Detroit, MI*

Detroit AT Study

A1

82.8

16.6

25

—

Si

28.3

5.7

110

—

S

12.5

2.5

1500

—

K

6.6

1.3

78

—

Ca

4.1

0.8

69

6.7

V

2.0

0.4

2

—

Cr

1.3

0.3

2

2.9

Mn

1.5

0.3

4

100.0

Fe

4.1

0.8

120

—

Ni

1.9

0.4

2

2.3

Cu

2.0

0.4

6

—

Zn

1.3

0.3

25

—

Cd

5.2

1.0

—

—

Pb

3.2

0.6

6

—

* Speciation Trenc

s Network data from October, 2001 to September, 2002 (Fourth External

Review Draft of PM Criteria Document, 2003).

SVOCs. A variety of particle phase SVOCs will be analyzed including PAHs, alkanes, hopanes
and steranes, organic acids, and sugars. Specific target compounds for this study include

•	Alkanes - C23 to C34

•	Petroleum biomarkers - norhopane, hopane

•	PAHs - pyrene, chrysene/triphenylenen, benzo[k]fluoranthene, benzo[b]fluoranthene,
benzo[a]pyrene,indeno[l,2,3-cd]pyrene, benzo[ghi]perylene

•	Organic acids - hexadecanoic acid, hexadecenoic acid, octadecanoic acid, octadecenoic
acid

•	Sugars - levoglucosan

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SVOCs will be removed from the filter using a validated solvent extraction technique, such as
sonication. The NERL is currently validating extraction, recovery and analysis procedures for
particulate phase SVOCs collected onto teflon filter media of various sizes, sampling volumes,
and sampling locations (community, residential indoors, residential outdoors). This work is
being performed in the NERL's Organic Analysis Laboratory. Organic acids and sugars will be
derivatized prior to analysis (using diazomethane or a similar compound). Filters will be spiked
with several deuterated internal standards prior to extraction to check recoveries in all samples.

SVOCs in sample extracts will be measured using GC-MS. Specific ion monitoring (SIM) of
select mass fragments for each of the target compounds will permit the establishment of
calibrated response factors. SIM is a fundamentally more sensitive approach in comparison to
total ion methods. Detection limits on the column should be 0.02 ng or less with estimated
detection limits of-0.01 ng/m3 (10 LPM for 24 hr sample) with 10-15% precision. Extraction
recoveries will be at least 80% and likely higher. Estimated performance results for selected
SVOCs are shown in Table 4-5.

Table 4-5. Estimated Performance Results for Select PAH SVOCs

Analyte

MQL

Mean Analytical

Analytical



(ng/m3)

Precision (%)

Recovery %

Pyrene

<0.30

13

> 85

Chrysene

<0.10

10

> 95

Benzo(a)anthracene

<0.10

11

> 95

B enzo(k)fluoranthene

<0.10

12

> 95

Benzo(b)fluoranthene

<0.10

10

> 95

Benzo(a)pyrene

<0.04

9.0

> 95

Indeno( 1,2,3 -cd)pyrene

<0.07

11

> 95

Benzo(ghi)perylene

<0.12

9.0

> 95

Table 4-6 shows typical ranges in air concentrations for the target analytes. Method performance
studies are currently under way. Standard operating procedures for this effort will be developed
once the method is finalized.

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Table 4-6. Estimated air concentrations for selected target SVOCs.

svoc

Compound Class

24-hr Environmental Concentrations (ng/m3)





Schauer & Cass 2000

Zheng et al., 2002

Season



winter

spring, summer,
winter, fall

Location



rural, suburban, and
urban sites in the San
Joaquin Valley, CA

rural/suburban and
urban cites in AL,
GA, MS, FL

nC23

Alkane





nC24

Alkane

2.04-42.3

0.02-3.97

nC25

Alkane

2.44-41.2

0.13-4.00

nC26

Alkane

1.49-29.9

0.30-2.87

nC27

Alkane

1.50-25.0

0.46-1.88

nC28

Alkane

0.78-12.3

0.28-1.41

nC29

Alkane

3.22-40.4

0.62-2.65

nC30

Alkane

0.28-7.39

0.16-1.08

nC31

Alkane

0.68-16.1

0.68-2.10

nC32

Alkane

1.98-3.71

0.06-0.42

nC33

Alkane

3.31-5.02

0.12-0.51

nC34

Alkane





norhopane

Petroleum biomarker



0.01-0.57

hopane

Petroleum biomarker



0.01-0.59

pyrene

PAH

0.05-3.28

0.02-0.56

chrysene/triphenylenen

PAH

1.2-7.7

0.03-2.55

b enzo [k] fluoranthene

PAH

0.04-8.69

0.06-2.75

b enzo [b ] fluoranthene

PAH

0.10-10.7

0.06-2.40

b enzo [a] pyrene

PAH

1.77-8.23

0.03-2.50

indeno[l,2,3-

PAH

2.56-6.84

0.04-1.58

cd]pyrene







benzo[ghi]perylene

PAH

3.49-9.75

0.04-2.18

hexadecanoic acid

Organic acid





hexadecenoic acid

Organic acid



0.1-1.7

octadecanoic acid

Organic acid





octadecenoic acid

Organic acid





levoglucosan

Sugar

22.5-7590

166-358

4.4.2 Nephelometer (personal DataRam®)

The NERL has successfully employed the MIE pDR (personal DataRam) nephelometer (MIE
Inc., Bedford, MA) in well over 1000 sample collections in four major field studies. In this
study, the pDR-1200 will be operated concurrently with personal, indoor, and ambient impaction
samplers. It is a portable optics-based monitoring instrument that uses light scattering to estimate
PM mass concentrations. The MIE provides real-time PM mass concentrations (-0.5 to 8 |j,m) on
a one-minute basis. It will be operated using a 2.5 |j,m size selective inlet and relative humidity

42


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controls to permit more optimized operation. The nephelometers will be calibrated by the
manufacturer using 2.5 |j,m dust particles; no additional field calibration will be performed.
Samplers will be zeroed prior to use and daily in the field. For personal monitoring, the MIE will
be placed in the sampling vests along with the PEM samplers. The nephelometers correlate well
(r> 0.8) with the PEM monitors (Tampa Asthmatic Children's Study).

This device has been determined to be robust and highly portable. Even though the instrument is
capable of reporting 0.01 |ig/m3 of PM mass concentration, field evidence has indicated that a
practical limit of detection of 1.0 |ig/m3 be used with a limit of quantification of 2.0 |ig/m3.
Precision of the instruments has been shown to be ± 10% from collocated instruments under a
variety of environmental conditions. Stored digital data will be recovered using the unit's RS232
data port and the manufacturer's software. Data will be recovered automatically from the
instrument using a macro-imbedded software program (Excel). The output from this spreadsheet
will provide minute-by-minute PM2.5 nephelometer mass concentration data ready for input into
the final analysis program (SAS).

The nephelometer data will be used to examine personal and indoor sources of PM and the
influence that personal activities have on PM exposures. Recovery of MIE data during each
measurement day will permit study technicians to investigate time-activity diary patterns with
episodes of observed high PM concentrations (5 highest peaks). Comparison of data from the
real-time devices (central site, personal and residential) will permit the direct determination of
source influence upon personal and residential settings). Infiltration factors and source strengths
will be calculated from the resulting data.

4.4.3 Elemental Carbon/Organic Carbon

Personal, indoor, outdoor, and central site samples will be collected for elemental carbon (EC).
PM2.5 EC will be collected onto Teflon filters at flow rates ranging from 2 to 16.6 LPM for 24
hours. These will be the same filters used to collect PM mass and SVOCs. An optical
transmittance procedure, currently being evaluated using data from two recent EPA studies (the
RTP Panel Study and the Tampa Children's Study) will be used to establish these EC
concentrations. The optical method is nondestructive, therefore allowing for these filters to be
used for the other primary analyses (e.g., extracted for SVOC content). Values of 0.11 and 0.34
|ig/m3 have been established for the LOD and MQL respectively, for the optical procedure.

The thermal optical analysis (TOA) method is a destructive EC and OC (Organic Carbon)
analysis technique. This technique is well established and typically involves the collection of
PM2.5 carbonaceous matter on a pre-fired quartz filter. The NERL is currently evaluating
experimental data pertaining to the collection, retention, and recovery of OC species on quartz,
teflon, and other media (e.g., denuders, electrostatic precipitators). The need for back-up
collection media associated with a primary quartz filter for OC is being evaluated based upon
some reported literature findings about this technique. It is anticipated that data from these
experiments will be fully evaluated prior to the start of field data collection. Based upon the
results of these ongoing studies, modifications to the basic quartz filter-based OC collection may
be required.

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The TOA procedure calls for a known portion of the used filter area to be recovered and placed
into a combustion chamber where it undergoes increased thermal heating under a controlled
state. Typically an inert gas, such as helium is used in this process. As a result of the thermal
heating, various carbonaceous fractions, such as the OC1 peak containing many of the more
volatile organic carbon species, are released from the combustion chamber, converted to simple
hydrocarbons and ultimately detected by an in-line flame ionization detector. The detection
limits for OC, OC1, and EC by TOA are listed in Table 4-7. Historical data has shown that well
over 95% of even the low volume (2 1pm) personal samples can meet this limit. Artifact issues
have been raised on the use of quartz filters for organic and total carbon for personal and indoor
exposure assessment (Landis et al., 2001). These artifacts have been associated with indoor
SVOC sources not related to ambient infiltration.

Table 4-7 EC-OC Detection limits

Measure

Analysis Technique

MDL (lig/m3)

MQL (|ig/mJ)

OC

TOA

1.18

3.53

OC1

TOA

0.48

1.43

EC

TOA

0.54

1.61

Optical EC

Optical transmittance

0.11

0.34

4.4.4 Criteria Gases

Personal and central site gaseous O3, SO2, and NO2 will be collected using Ogawa diffusion
badges. The Ogawa badge is a passive sampler containing a coated filter supported by two
screens with a diffusion barrier end cap (Koutrakis et al., 1994). The filter substrate will be
coated with either sodium nitrate to retain O3 or triethanolamine to retain SO2 or NO2 where the
criteria gas stoichometrically reacts with the substrate and is retained.

All of the Ogawa badges are then extracted and the resulting ion quantified using ion
chromatography. This methodology has been used by the NERL for almost 10 years and has
been highly successful in the passive determination of ambient gas concentrations. Details on
detection limits, quantitation limits, blanks and duplicates for these gases in the TACS are shown
in Table 4-8. These badges have been shown to be highly successful in the collection of ambient
samples, however, low personal exposure concentrations, especially for ozone, often result in
concentrations at or below the limit of detection. It is expected that similarly low concentrations
may occur in the Detroit homes. Indoor concentrations are expected to be highly dependent upon
the participant's use of natural ventilation.

Table 4-8. NQ2, S02 and 03 completeness and

detection limits (pp

3) from the TACS.

Analyte

Completeness
(%)

MDQ

MQL

Blanks

Duplicates









N

mean

SD

N

Mean

SD

N02

97

0.3

1

3

8.3

0.4

2

0.9

0.5

S02

97

7.6

22.8

4

6.3

10.8

2

6.4

8.1

03

97

8

24

4

24.1

16.6

2

-1.4

3.4

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4.4.5 Nitrate

Nitrate in central site, outdoor, and indoor air samples will be collected using mini denuder
samplers. Samples are collected on 15 mm diameter sodium carbonate-coated glass fiber filter at
0.8 LPM. To minimize the effect of acidic ambient gases upon the PM collected on the filter, the
sample stream first passes through two sodium carbonate-coated mini-denuders. These samplers
have been successfully used as part of the ChemPass Model 3400 Personal Sampling System
developed by the Harvard School of Public Health and available through Rupprecht &

Patashnick Co., Inc. (Albany, NY).

Nitrate is extracted from the filter samples with distilled deionized water for 30 min. The extract
is then analyzed by ion chromatography. The reported method detection limit, 0.26 ng/m3, is
well below reported ambient air concentrations in Detroit. Precision ranges from 11.3-14.6% for
the method based on analysis of duplicate samples.

4.4.6 Carbonyls

Formaldehyde, acetaldehyde, and acrolein will be collected using a passive aldehyde and ketone
sampler known as PAKS. This method is a diffusion based passive sampler originally developed
for use in the RIOPA study for the analysis of formaldehyde and other aldehydes and ketones.
The PAKS method is currently being optimized for the collection and analysis of acrolein while
maintaining its effectiveness for the measurement of other carbonyls such as formaldehyde and
acetaldehyde. The PAKS method utilizes a Ci8 cartridge coated with dansylhydrazine (DNSH)
which reacts with carbonyls to form stable DNSH-carbonyl derivatives. The DNSH-carbonyl
derivatives are solvent extracted with acetonitrile and analyzed by HPLC with fluorescence
detection. A sampling time period of 24 hours is targeted for this study. Preliminary quality
assurance results from its use in the Tampa Asthmatic Children's Study are presented in Table 4-
9.

Table 4-9. Performance Results for the PAKS Method from the Tampa Asthmatic Children's
Study (48-hr samples).

Analyte

MDL

(Hg/m3)

Analytical
Precision

Source

Analytical
Recovery (%)

Formaldehyde

0.19

5.4%

lab, field blanks; duplicate

101 ±4







samples



Acetaldehyde

0.46

5.3%

lab, field blanks; duplicate

87 ±3







samples



Acrolein

0.47

9.5%

lab, field blanks; duplicate

60 ±2







samples



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4.4.7 VOCs

The method proposed for VOC collection and analysis is currently under development at EPA.
It uses passive samplers containing Carbopack X as the collection media and thermal
desorption/GC/MS as the analysis method. This method is intended to overcome performance
issues for 1,3-butadiene that have been observed with other passive methods. Preliminary testing
indicates that the Carbopack X (graphitized carbon black) sorbent will be suitable for the
collection of 1,3-butadiene as well as the other listed target VOCs over the proposed 24 hour
monitoring period.

Several configurations of the diffusion based passive samplers are being investigated including:
Perkin-Elmer stainless steel tubes, SKC Ultra Badges, and a "Monsanto" large surface badge.
Primary differences in these devices are effective sampling (or diffusion) rates and the procedure
for thermal desorption. Perkin-Elmer tubes exhibit the lowest effective sampling rates (~ 1
mL/min or less) and are directly desorbed using a Perkin-Elmer thermal desorption interface.
The SKC badges exhibit a moderate effective sampling rate (-10 to 15 mL/min) and require that
the sorbent be transferred from the sampling badge to a Perkin-Elmer tube and then analyzed
using the Perkin-Elmer interface. The "Monsanto" badge effective sampling rate has not been
determined but is expected to be the highest. Monsanto badges are directly desorbed and
injected into the GC/MS using a specifically designed and built interface for these badges.

The three collection devices will be evaluated and the method selected will be based on a number
of parameters including sensitivity, recovery, effective sampling rates, precision for the target
compounds, ease of use and analysis, ruggedness, and availability of materials. A representative
number of collocated canister samples will be employed to assist in the validation of this new
methodology.

Typical urban concentrations of 1,3-butadiene are in the range of 0.3 to 1.6 //g/m3. The limit of
detection for this method is currently being defined but is expected to be in the sub Mg/m3 range.

4.4.8 Air Exchange Rate Measurements

Air exchange will be measured in each study residence to determine the integrated air infiltration
rate during the monitoring period. Residences will be treated as a well-mixed one-compartment
model. Permeation devices (emitters) containing a perfluorocarbon compound will be placed
throughout each residence 24 hours before monitoring. The perfluorocarbon tracer (PFT) is
emitted at a known rate that can be adjusted for temperature. The tracer mixes with the indoor
air in the house. Tracer gas concentrations measured in residences will be dependent on the
house volume and the rate of outdoor air infiltration. The tracer compound will be collected by
diffusion using capillary adsorbent tube samplers (CATS) placed inside the residences for
monitoring. One CAT will be placed in the central living area. Samples are collected when the
adsorbent tube is uncapped. At the end of each 24-h monitoring period, the samplers will be
capped, stored in sealed aluminum cans at room temperature, and shipped to Brookhaven
National Laboratory for analysis. The amount of adsorbed PFT is determined by gas
chromatography with electron capture detection. The PFT concentration (C) will be derived

46


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from the amount of adsorbed PFT (A), the sampling period (t), and the adsorption rate of PFT by
the collectors (r):

C(pft)= A/rt.

The air infiltration rate (E) will be calculated from the volume of the residence (V), the source
emission rate (S) and the number of sources (N):

E (1/h) = NS r t/A V.

These samplers have been shown to be reliable with low blanks and minimal contamination.
Recovery of spiked controls is usually close to 100% with 4% precision.

Historical limits of detection for this procedure have been on the order of 0.2 exchanges/h with a
precision of ± 10%. Extensive use of this technique in recent NERL panel studies
(approximately 1000 measurements), have resulted in a sample QA completeness record
(environmental samples above the quantification limit) of over 95%.

4.4.9 Supplemental Central Site Monitoring

Air sampling at the central site monitor will include a suite of monitors to collect additional
samples for ultrafines, PAHs, trace metals, and soluble metals. Ultrafine PM will be collected
using a SMPS/LASX sampling system. Additional filters will be collected for trace metal
analysis, soluble metal analysis, and PAH analysis.

Particulate mass at the central monitoring site will also be collected using a Sierra Anderson
dichotomous sampler (or comparable monitor). The dichotomous design provides both fine and
coarse measures. The dichotomous sampler draws air at a flow rate of 16.70 LPM through 37
mm Teflon filters. Ninety percent of the air (15.03 LPM) flows through the fine particulate filter
and the remaining 10 percent (1.67 LPM) flows through the coarse particulate filter. The
dichotomous sampler uses a virtual impactor (region of stagnant air) to segregate the air sample
into two fractions. The virtual impactor particle separator accelerates the air sample through a
nozzle and then deflects the air at a right angle. Most particles smaller than 2.5 |iin (fine
fraction) will follow the higher air flow path and collect on a fine particulate filter. Particles
between 2.5 and 10 [^m (coarse fraction) have sufficient inertia to impact into the chamber below
the nozzle and are collected on a coarse particulate filter. Ten percent of the sample air flows
through the coarse particulate filter and because of this approximately 1/10 of the fine particles
are collected on the coarse particulate filter. Both coarse and fine particle samples will be
analyzed for mass, EC, elements, and SVOCs as described in Section 4.4. 1.

Meteorological variables will be collected daily at the central monitoring site. These data include
real-time wind speed, wind direction, relative humidity, and temperature for each measurement
day. Historic weather data from the central site is available so that common weather regimes can
be defined to permit extrapolation of study data. Back-trajectories will be established using the

47


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meteorological data to match personal or residential source findings with known industrial
facilities or other pollutant sources.

4.4.10 Supplemental Traffic Counts

Vehicle activity on the road can have profound effects on the concentrations and characteristics
of pollutant impacts in the vicinity of the roadway. To account for vehicle activity on temporal
variability of the pollutant measurements, daily traffic count data will be obtained from the
Detroit transportation planning agency (SEMCOG) and the Michigan Department of
Transportation (MDOT) for all available roads in the vicinity of the monitoring sites. An attempt
will be made to install traffic count monitors on the nearby major roadways during residential
monitoring periods, as available through the MDOT. When direct vehicle count measurements
are not available, traffic volumes will be estimated by traffic demand modeling conducted by
SEMCOG personnel. Fleet characteristics (percent light-duty and percent heavy-duty) data will
also be obtained from SEMCOG for these roadways. When feasible, video recordings will be
conducted on all major roads in the vicinity of the monitoring sites. The video will be analyzed
to determine flow rates and fleet mixes on the roadway during sampling events. Software will be
used to interpret the video images for eight vehicle class categories (including light, medium and
heavy duty diesel and gasoline vehicles).

4.5 Survey Instruments

Five questionnaires will be developed and submitted to the Office of Management and Budget
(OMB) for approval. These include a Participant Characteristic Survey, a Technician Survey
collecting data on household characteristics and local ambient sources, a Daily Follow-up
questionnaire concerning that day's activities, particularly those related to sources as determined
from a real-time examination of the continuous MIE results, and a Time Activity Diary that the
participant carries with him and fills out regarding his location and activities (Table 4-10). The
draft questionnaires and survey instruments are attached in Appendix D. These instruments have
been developed as a result of field use in a series of EPA human exposure panel studies. The
Time Activity Diary and the Technician Surveys are similar documents used in previous studies.
These are scannable forms which allows fast recovery of the raw data and improvements in the
data validation. These documents and the corresponding data have been shown to be practical,
low burden, and directly applicable to the stated goals of this project. The Daily Follow-up
Questionnaire is now in a computer assisted survey format to reduce data collection errors and
decrease data recovery time. Evaluation of the survey instruments from the Tampa Asthmatics
Children's Study version has resulted in additional improvements in the form that should further
improve data quality (reduced missing entries, increased valid entries, and consistent responses).

48


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Table 4-10. Survey Instalments

Instrument Type

Administered

Information Gathered

Participant Characteristic
Survey

Completed at the time of informed consent by
study technician

Basic information such as the age, height,
weight, and occupational status of the
participant

Technician Surveys (2)

At start of monitoring period each season;
completed by technician observation

Housing location, building characteristics,
ventilation, cooking/fuel characteristics,
room characteristics, local ambient sources

Daily Follow-up
questionnaire

Daily at end of monitoring period; technician
administers to participant

Potential sources (i.e., cooking, smoking,
cleaning) in home each day, ventilation
characteristics

Time Activity Diary

Participant completes diary throughout the day;
technician reviews and verifies entries daily at the
end of the monitoring period. Output from the
personal nephelometers will be downloaded each
day; participant will be queried about locations
and activities during times that show high PM
exposures.

Information on participant's location,
activities, and proximity to smoking or
cooking, recorded for every 15 minutes

4.6 Pilot Study

A 9-person pilot study was performed in Tampa, FL. Households were monitored for four
consecutive days using many of the techniques that will be employed in the DEARS. (Certain
new methods such as those for the VOCs and SVOCs will be extensively tested in the laboratory
and to some degree in the field before adopting them for use in the DEARS.) The Tampa study
data are still being analyzed. However, the methods have been evaluated and found for the most
part to be well suited for use in field studies. A limited period (2-3 days) of technician field
training will be employed at the start of the study that will involve collection of field samples.
This will provide the RTI, International staff the opportunity to become fully familiar with any of
the new sample collection methodologies employed since their last EPA assignment (e.g., new
survey questionnaires).

5.0 STATISTICAL ANALYSIS PLAN

Our analysis approach will be tied to the objectives discussed in Section 2 above. That is,
univariate statistics to compare personal, indoor, residential outdoor and central site
concentrations of the target pollutants, together with testing to if determine differences exist
between locations. Cross-sectional correlations between types of measurements (e.g., personal
vs. central site) will be determined, as will correlations among target pollutants within one type
of measurement. Longitudinal correlations (e.g., personal vs. indoor, indoor vs. outdoor, etc.)
will be determined for each subject across the 5 days per season, and, if appropriate, across the
full 10 days covering both seasons. The infiltration factor governing the fraction of outdoor
aerosol or air toxic contributing to the indoor concentrations will be determined using one of
several methods (See below).

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Following elemental and chemical analyses of the speciated compounds, source apportionment
models such as PMF and/or UNMIX will be run in an attempt to identify the major source types
affecting exposure using personal PM and SVOC measurements. Relationships between personal
exposure and indoor/outdoor concentrations identified from the study results will provide
information needed for development of predictive models of exposure such as NERL's
Stochastic Human Exposure and Dose Simulation (SHEDS) models for PM and air toxics.
Exposure model algorithms and inputs for PM constituents and air toxics will be improved and
refined based on the DEARS measurements and data analysis results.

5.1	Preliminary analysis of existing monitoring data

5.1.1 Sources of monitoring data

PM, air toxics, and related environmental data collected from recent (non-DEARS),
measurements will be analyzed to strengthen the current study design while awaiting OMB study
clearance. Data sets included in this analysis will include those from Wayne County air toxics
sites, select Detroit Air Toxic Pilot Study measurement and potentially data from local academic
research groups (e.g., such as that of Gerald Keeler, University of Michigan). Analyses
performed upon this data (simple univariate statistics as well as more sophisticated factor
analysis) will be used to strengthen the ultimate selection of census tracts incorporated into the
participant recruitment process by helping to further establish the dominant pollutant source(s) in
a given area. It will also serve to help guide any additional measurement needs prior to field data
collections (e.g., the value of a specific VOC relative to cluster analysis).

5.2	Analysis of study data

Univariate statistics will be employed for the personal, indoor, residential outdoor, and central
community site data. Assuming lognormal distributions, the geometric means of the different
sites, population groups, and types of samples (e.g., indoor vs. outdoor) will be compared using
Student's t test or other appropriate metric. Non-parametric tests will be used if the distribution is
not lognormal.

Correlations (both Spearman and Pearson) of personal exposure and indoor concentrations with
the residential outdoor and central site data will be performed. A mixed-effects regression model
will be performed to identify the influence of community sources on indoor and personal
exposures, the infiltration factor as a function of measured air exchange, and the attenuation
factor affecting personal exposures based on activities.

Using the PM, gases, carbonyls, VOC, elements, and SVOC data, a source apportionment model
or models (PMF and/or UNMIX) will be run to identify and quantify the contributions to and
uncertainties associated with ambient and indoor air of various types of sources, including diesel,
gasoline exhaust, possibly gasoline vapor, stationary source combustion, resuspended crustal
material, and other major sources. Two ways to extend the types of models analyzed are to use
transformed variables and/or non-identity link functions in the generalized linear models.

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The data analysis plan is summarized in Table 5-1 where each study question is restated, the data
that will be available to address the objective are summarized, and the data analysis approach is
given.

Data analysis will occur in four different phases.

Phase 1. This phase will focus primarily on calculating descriptive statistics (i.e. means, standard
deviations, etc.) along with frequency histograms and box plots of personal, indoor, outdoor,
central site measurements for PM2.5, PMcoarse, gases, elements, VOCs, SVOCs, and EC-OC and
the other pollutants. This will serve not only to summarize the data but provide an exploratory
approach to look for anomalies in the data as well. In addition to determining univariate
summary statistics, time series plots of the results will also be used for preliminary analysis.

Relationships between paired daily personal PM exposures and indoor, outdoor, and
ambient concentrations will be evaluated for each metric and participant using simple
linear regression, and Pearson correlations. For these analyses, data will be stratified
by season and geographical location. Ratios, xy-plots along with correlation tables will
be used to examine potential relationships.

General linear and mixed models will be used to determine the influence of activity
patterns and housing characteristics on relationships of residential indoor/outdoor
particle/gas concentrations to those measured at the central site monitor. Information
from these analyses will be used to identify important variables for possible inclusion in
linear models. Random coefficients models will not be appropriate since individuals
selected for the study are not chosen at random; however data on subjects can be
included as fixed effects in the model. General linear mixed models will be use to
account for possible correlations in residuals that result from the repeated
measurements. Homogeneity of variance will be examined before pooling the data for
analysis using general linear models.

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Table 5-1. Data analysis plan
Analysis Objective

1. Determine the associations
between concentrations
measured at central site
monitors and outdoor
residential, and indoor
residential and personal
exposures for selected air
toxics, PM constituents and
PM from specific sources.

DEARS.	

Measurements Required

Personal, indoor, outdoor, and
central site measurements for
PM2.5, PMcoarse, elements, air
toxics, and other pollutant
variables. Stratified by site,
season, housing stock,
geographical location, and
primary source influence.

52

Proposed Analysis

Univariate statistics on
personal exposures, indoor,
outdoor, and central site data
by site, season, subpopulation.
Box plots, histograms, time
series plots, and other
preliminary and exploratory
data analyses and graphics
methods. Linear regressions,
correlations

(Pearson/Spearman), ratios
between personal, indoor,
outdoor, central site data.

Analysis Results and

	Interpretations	

Box plots and histograms show
visual evidence of skewness and
other departures from normality,
relative shapes, central
tendencies and dispersions of
the measurements collected
under different spatial and
temporal conditions. Cartesian
xy-plots show relative scatter
and potential linear
relationships, and correlations
between indoor, outdoor and
ambient data. Differences
between mean values
hypothesized to be equal under
the null hypothesis (H0) and
between slopes/intercepts or
correlations (assumed to be
zero) will be tested for statistical
significance (p < .05) using
appropriate statistical tests.


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2. Describe the physical and
chemical factors that affect the
relationship between central
site monitors and outdoor
residential and indoor
residential concentrations,
including those that affect
ambient source impacts.

Covariate measurements
consisting of meteorology data,
community sources, air exchange
rates, house structure, house
ventilation parameters, indoor
sources, participant locations,
participant activities, real-time
personal, indoor and outdoor PM
data, elements, and air toxics.

General linear and/or mixed
models will be used to examine
the effects and influences of
external sources of variation on
personal exposure relationships
with indoor/outdoor and central
site measurements.

Significant (p < .05) differences
in linear slopes and intercepts
due to subjects, sites, seasons, or
uncontrolled external influences
hypothesized (under H0) to be
zero will be determined by
appropriate statistical tests.

Tests for positive auto-
correlation between repeated
measurements resulting in
under-estimation of slopes in
linear relationships will be dealt
with using SAS mixed model
analysis and/or testing at more
conservative p-levels (e.g. by
setting p < .01).

3. Identify the human activity
factors that influence personal
exposures to selected PM
constituents and air toxics.

Personal, indoor, outdoor, and
central site measurements for
PM2 5, PMcoarse, elements, air
toxics, and other pollutant
variables. Stratified by site,
season, housing stock,
geographical location, and
primary source influence. Time
activity and household surveys.

General linear models will be
used to establish relationships
following integration of
exposure and time activity
databases.

Results will be based on least
squares and maximum
likelihood methods to fit linear
models and test the statistical
significance (p <05) of the
difference between slopes that
are hypothesized to be equal
under H0 regardless of the
individual activity patterns.

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4. Improve and evaluate
models used to characterize
and estimate residential
concentrations of and human
exposures to selected air
toxics, PM constituents, and
PM from specific sources.

5. Investigate and apply
source apportionment models
to evaluate the relationships
for PM from specific sources
and to determine the
contribution of specific
ambient sources to residential
concentrations and personal
exposures to PM constituents
and air toxics.

Personal, indoor, outdoor, and
central site measurements for
PM2.5, PMcoarse, elements, air
toxics, and other pollutant
variables. Stratified by site,
season, housing stock,
geographical location, and
primary source influence. Time
activity and household surveys.

Personal, indoor, outdoor, and
central site PM measurements.
Incorporation ofVOC, SVOC,
carbonyl, elemental and other
speciated data.

54

Validation, maintenance, and fit
of population exposure models
using output from emissions-
based atmospheric dispersion
models will be determined on
the basis of statistical
significance (p <05) of changes
in (R2); the percent personal
exposures variation explained as
a result of the of the reduction

in pollutant emissions.	

Mass-balance/Source	Source impacts and

apportionment models such as contributions will be quantified
UNMIX or PMF.	with effective variance least

squares chemical mass balance
results. The most probable
combination of sources that
significantly account for percent
variation of mass explained will
be determined interactively by
adding or subtracting variables
in multiple regression and/or by
changing the number of
principal components in factor
analysis.

Development of models, such
as the SHEDS-PM, using
actual field data as primary
inputs.


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6. Determine the associations
between central site
concentrations of criteria
gases (O3, NO2, and SO2) and
personal exposures for these
gases as well as personal
exposures to air toxics, PM
constituents and PM from
specific sources.

Personal, residential and central
site criteria gas concentrations.
Personal, indoor, outdoor, and
central site PM measurements
along with VOC, SVOC,
carbonyl, elemental and other
speciated data.

55

Univariate statistics on
personal exposures, indoor,
outdoor, and central site data
by site and season. Box plots,
histograms, time series plots,
regression analyses, other
preliminary and exploratory
data analyses and graphics
methods. Linear regressions,
correlations

(Pearson/Spearman), ratios
between personal, indoor,
outdoor, central site data.
Mixed models will be used to
examine the relationships
between concentrations and
exposures of criteria gases and
PM2.5, PM constituents, and air
toxics.

Box plots and histograms show
visual evidence of skewness and
other departures from normality,
relative shapes, central
tendencies and dispersions of
the measurements collected
under different spatial and
temporal conditions. Significant
correlations between indoor,
outdoor and central site data will
be used to determine the
important parameters for mixed
model anlaysis. Differences
between mean values
hypothesized to be zero under
the null hypothesis (H0) and
between slopes/intercepts and
correlations will be tested for
significance (p < .05) using
appropriate statistical tests.

Tests for positive auto-
correlation between repeated
measurements will be tested
using appropriate statistical
tests.


-------
Phase 2. To achieve adequate predictions for indoor concentrations, factors affecting
outdoor levels must be considered. The effects of different site locations, local weather
conditions, traffic patterns, and local outdoor sources along with air exchange rates can
be used to categorize the data. T-tests can be used to determine if homes in categories
with sources are different from homes with no direct influence from known outdoor or
indoor sources. General linear and mixed models will be used to determine the
influence of activity patterns and housing characteristics on relationships of personal
particle/gas exposures to indoor/outdoor concentrations. Information from these
analyses will be used to identify important variables for possible inclusion in linear
models. Random coefficients models will not be appropriate since individuals selected
for the study are not chosen at random; however subjects can be included as fixed
effects in the model. General linear mixed models will be used to account for possible
correlations in residuals that result from the repeated measurements. Homogeneity of
variance will be examined before pooling the data for analysis using general linear
models.

Phase 3. Validated data and information gained in phases 1-2 will be incorporated into
the PM human exposure modeling development. This will be an ongoing and iterative
process over the course of the full study period. New datasets will be incorporated as
they become available and the model refined at each step. Existing models, such as the
SHEDS-PM, will be used as the foundation of the initial effort. A goal of this phase
will be to develop a model that will be versatile and translatable to other metropolitan
sites.

Phase 4. Data obtained in phases 1-2, along with original environmental measurements
will be used to perform source apportionment modeling. The goal of this phase will be
to establish the dominant sources that impacted personal, residential and central site-
based measurements and the contribution of each primary source upon each spatial
setting. Modeling tools such as UNMIX and PMF will be used to support this phase.

5.2.1 General Linear Models Analysis

The specific type of statistical analysis to be performed on the data is dictated by the
objectives of the study, and the study design. One fundamental objective of the study is
to examine the relationships (with respect to mobile and stationary sources), between
residential (household) PM/gas measurements and central site concentrations of PM/gas
over a 5-day period (or a 10-day period covering two seasons) for each of the 120
homes monitored. Assuming this is a linear relationship, these relationships can be
expressed in terms of a simple linear regression model as follows:

Yy = a, + p,X, + sy,	(1)

i = 1,2,	,120 j = 1, 2,	10 days,

where

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Yy = personal PM/gas measurement for the ith household on the jth day ,
a; = intercept for the ith household,

Pi = slope for the ith household,

Xj = either the indoor, or outdoor, or central site PM/gas concentration on the
jth day, and

Sij = residual error (random) for the ith household on the jth day.

This basic regression model will be fitted for each individual participant, home, and for
each of the PM/gas metrics. The coefficient of determination (R2) along with the p-
values to examine the significance of the intercepts and slopes, will be calculated for
each relationship. Measurements on the same person taken sequentially and close in
time may be correlated. This results in a correlation between residuals on adjacent days.
This will be accounted for in the error variance matrix and will primarily involve first
order correlation />, between residuals one day apart, with correlations of p2, p\ etc. for
residuals 2, 3, etc. days apart. Ignoring a positive autocorrelation will lead to an intra-
personal variance that is too large. In general (see discussion below), the variance
between groups of participants (i.e., inter-personal variance) will be underestimated and
the variance across time will be overestimated. Correlated outcomes must be addressed
to obtain valid estimates.

As discussed earlier, there will be a total of at least 120 households being measured
repeatedly for 5 days in each of 2 seasons. Along with possible seasonal effects on the
relationships, other factors that may or may not statistically affect the relationships are
related to the categories in which the participants will be placed based source impacted
categories. Other factors included in the study design that possibly could affect the
relationships are distance from source, meteorology, and residential type (apartment,
stand-alone homes). As illustrated above, the regressions will be calculated separately
for each individual participant within each season. Statistical comparisons of slopes and
intercepts within and between distance from sources, seasons, housing types, etc. will
be performed by comparing individual results on a case-by-case basis within groups
and by comparing household group averages of intercepts and slopes using pooled
within-group estimates of intra-household variation as the basis for statistically testing
and measuring the separation between group means. A more general linear model that
combines both categorical variables as well as continuous variables into a single
comprehensive model and allows for multiple comparisons with the aid of analysis of
variance techniques is given as follows:

Yljkl = n + Si + Pj + Xk + S*Pij + S*Xlk + P*Xjk + S*P*Xljk + Eijk ,	(2)

i=l,...,2 j = l,	,120 k=l,	,5

where

Yijki = personal PM/gas exposure for the jth participant on the kth day during the
ith season,

[i = overall mean,

S; = effect of ith season (fixed),

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Pj = effect of jth household (fixed),

Xk = PM/gas measurement at indoor, or outdoor, or central site on the kth day
S*Py = effect of interaction of the ith season with the jth participant (fixed),

S*Xik = effect of the ith season on the slope (fixed),

P*Xjk = effect of the jth participant on the slope fixed),

S*P*Xyk = effect of the ith season and jth participant on slope (fixed), and
Eyk	= random error on the kth day during the ith season for the jth

participant.

Using SAS, appropriate sums of squares will be generated to test hypotheses linked to
the study objectives. For example, the term P*X provides an additional sum of squares
due to the different regression coefficients for the individual homes. Since individuals
are not chosen at random, the slopes for Xk are considered to be fixed effects.

Therefore, a random coefficients analysis using mixed models is not appropriate.
However a mixed model procedure will be used to account for intra-personal
correlations due to repeat measures across time within seasons. Homogeneity of
variance across seasons and households will be examined and appropriate adjustments
and assumptions made.

The test corresponding to the sum of squares provided by the source of variation X, is a
test of the significance of the regression of PM/gas personal exposure (Y) onto X
(indoor, outdoor, or central site concentrations) ignoring groupings due to seasons or
households. Other tests include a test of whether or not seasons significantly alter the
regression coefficients, a test to detect inter-personal variability, and a test on proximity
to mobile or stationary sources. As stated above, homogeneity of variance should be
examined as well as the possibility of autocorrelation among residuals due to repeat
measurements on the same individual over time. Since relationships will be determined
for central site versus indoor, central site versus outdoor, central site versus personal,
indoor versus outdoor, and indoor versus personal, the results of these relationships can
be compared.

An over-parameterized linear model is require to further examine the effects of
household grouping by potential source impacts, residential types, proximity to sources,
and meteorology and the effects these factors have on the linear relationships as well as
seasonal effects, and various interactions between factors in a single analysis. These
additional groupings apply to the original 120 households so the size of the study is not
increased. This may lead to an imbalance in the design. The number of males versus
females or the number of households from a particular housing stock will be
determined during recruitment. A general linear model that incorporates these
categorical groupings as main effects and 2-way interactions along with the continuous
variable X (i.e. indoor, outdoor, and community PM/gas) and its interactions with the
main effects and 2-way categorical interactions is given below.

Yjjkimn = (J, + S; + Cj + Gk + Hi + Rm + Xn + S*Cij + S*Gik + S*H;i +S*R;m + C*Gjk +
C*Hj! + C*Rjm + G*Hki + G*Rkm + S*Xin + C*Xjn + G*Xk„ +H*Xln + R*Xmn + (all 3-
way interactions involving X) + Residual,

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i=l,..,2

j = 1,4 k = 1,2 1 = 1,2 m = 1,2 n=l,...,5,

where

Yijkimn	= personal PM/gas exposure during ith season, in jth cohort, for kth

housing stock, with 1th source proximity, mth residential status, on nth day,

[i	= overall mean

S;	= effect of ith season,

Cj	= effect of jth cohort,

Gk	= effect of kth housing stock,

Hi	= effect of 1th source proximity,

Rm	= effect of mth residential type,

Xn	= indoor, outdoor, or central site PM/gas measurement on the nth day,

S*Cij	= effect of interaction between ith season and jth cohort,

S*G;k	= effect of interaction between ith season and kth household group,

S*H;i	= effect of interaction between ith season and 1th source proximity,

(plus other 2-way and 3-way interactions with the continuous variable X), and

Residual = variation due to all higher order interactions + model error.

Variations of this more general model could include other factors or covariates such as
spatial groupings of participants or temperature ranges to group measurements instead
of seasons or activity patterns. Using SAS, model terms such as R*Xmn will generate
appropriate statistics for estimating and testing different regressions of Y onto X for
different groups. For this particular term, the relationships between central site
exposures and either indoor, outdoor, or personal PM/gas measurements will be
compared for different residential types. The effect of different factors on relationships
can be examined by testing the change in R2. To do this the amount of variation in the
Y variable accounted for by fitting the full model is compared to the amount of
variation in the Y variable accounted for by fitting a reduced model by leaving out the
term in question. The contribution of these "left out" effects to the full model is
determined statistically with the F-distribution. Graphical plots and visual inspection of
the trends and outliers in the data and model residuals will also be used to evaluate the
model fit.

This approach applies to any of the above linear models. Statistical significance is a
necessary but not sufficient condition for practical significance. Statistical significance
indicates that something other than chance is operating, but does it really matter from a
practical standpoint? Research studies such as this one with large sample sizes often do
find statistically significant results that are not of practical importance. Since R2 is not
affected by sample size it should also be reported as a measure of adequacy in fitting
each term in the linear model.

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The summary in Table 5-1 is included to ensure that the data collection plan and data
analyses are linked to study objectives and hypotheses. Analysis of the study data will
not necessarily be limited to those described in the Table. As in any study, additional
analysis needs and methods will be identified as the study is performed and the data are
examined.

5.3 Exposure Modeling

The measurement data collected during this study and the results of the data analysis described
above will provide critical new information needed for further development of population
exposure models for PM and air toxics. Population exposure models provide a method for
estimating exposures for a population of interest when no measurements or limited
measurements of personal exposure exist. These models are useful tools for predicting the range
in exposures across a population and the likelihood of exposures above a particular level. When
output from an emissions-based atmospheric dispersion model is used as input, population
exposure models are also useful for estimating the reductions in exposures that would occur as a
result of reductions in pollutant emissions.

The development of algorithms and databases needed to predict exposures requires as much
information on the factors that influence exposures as is available. The DEARS will provide
new information on the factors that influence the relationships between outdoor and indoor
concentrations of and human exposure to PM constituents and air toxics. This information will
be used to improve and refine the algorithms and input databases for population exposure
models. The measurements of personal exposures collected during the DEARS will also allow
for evaluation of the exposure model predictions. The outcome will be refined and evaluated
exposure models for PM and air toxics that are available for application to other metropolitan
areas.

NERL's Stochastic Human Exposure and Dose Simulation (SHEDS) models use a probabilistic
approach to predict the distribution of exposure and dose for a specified population. The SHEDS
models for PM and air toxics estimate this distribution by simulating the time series of exposure
and dose for individuals that demographically represent the population of interest. US Census
data are used to build the simulation population, and human activity pattern data are assigned to
each simulated individual to account for the way people interact with their environment.

Pollutant concentrations in the microenvironments that people spend time in (e.g., home, car,
office, school, restaurant, etc.) are calculated based on relationships between central site outdoor
and indoor or in vehicle concentrations obtained from measurement study data. Each
individual's exposure and dose profile is estimated from the time spent in each location, the
concentration in that location, and the activity-specific inhalation rate while in that location.
Daily-averaged exposure and dose for each individual are calculated and combined to provide a
distribution of exposure and dose for the population. Statistical methods for incorporating both
variability and uncertainty in the model input parameters are utilized to obtain the predicted
population distribution and the uncertainty associated with the predicted distribution.

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The exposure modeling component of the DEARS will include three phases. Initial
model development will occur prior to the completion of the measurement study and
case study applications will be performed with the SHEDS models using available data
on PM components from the Detroit Speciation Trends Network (STN) sites and on air
toxics from the Detroit Pilot Air Toxics Network sites. Results from NERL's DEARS
will then be used to improve and refine the algorithms and input databases. In this
second phase, the exposure model predictions for the DEARS will be evaluated against
the measurements of personal exposure for PM components and air toxics. Lastly,
further model development will involve incorporation of source apportionment results
into the exposure models so that the SHEDS models will be able to predict population
exposures to specific sources.

5.4 Source Apportionment Modeling

Data from the Particle Total Exposure Assessment Methodology (PTEAM) study and the 1998
Baltimore Particulate Matter Epidemiology-Exposure Study (BPMEES) were analyzed with
advanced receptor models to identify and quantify the sources of particulate matter collected on
personal, indoor, and outdoor samples. In addition, the planned receptor model development and
analysis of the Research Triangle Park Exposure Study data (NERL) will evaluate the approach
that will be used in the DEARS. A discussion of these studies and the modeling approach are
given in Appendix C.

5.4.1 Detroit Receptor Modeling

A modified 2-way factor analytic model will be used to analyze the DEARS data. The
Multilinear Engine technique (the program ME-2) will be used, incorporating the following
additions to the standard non-negative PMF 2-way model:

1.	Divide the factors in two groups, so that there is a group of "indoor only" factors that
only explain indoor and personal data, not outdoor or ambient. (This technique was used
in the Baltimore study.)

2.	The factor analytic model should not attempt to explain irregular non-recurrent features
of the data. Elements like Cu and Zn displayed such behavior in the Baltimore study, but
there may be other such elements. Such elements should be fitted with a dynamic
reweighting according to the following scheme:

-	data points with residuals more positive than a chosen cutoff limit (+0.5 sigma,
say) are considered "irregular" and downweighted analogously to the PMF
outlier downweighting scheme (Paatero 1999)

-	all other data points would be given the standard PMF/ME-2 weighting.

This scheme will fit regular behavior of the chosen elements in the normal way. However, stray
positive excursions will not influence the factorization. Such excursions will be inspected
separately, outside of the factor analytic model. The purpose of the suggested technique is that a
reasonably good fit is achieved with a small number of factors. Otherwise, additional factors
would be needed in order to fit the irregular behavior, and these extra factors would introduce
undesirable rotational ambiguity in the factor analytic model. It should be emphasized that the

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irregular positive excursions are not considered "bad data." Instead, it is recognized that such
data values cannot be successfully analyzed within a factor analytic framework.

3.	Implement closure constraints in the model so that the sum of all identified mass
(including oxygen etc. in certain compounds) cannot exceed the mass coefficient in any
factor. (This technique was used in the Baltimore study.)

4.	Include constraints that prevent some specific factor(s) from explaining certain
individuals/regions/times. This approach depends on the interpretation of factors and may
not be possible at all. An example is a localized source that only affects individuals living
in a certain suburb. Such a factor should be excluded from explaining individuals who do
not live (or visit) in the suburb in question. The use of constraints should be coupled to
supplementary information about the activities of the participants, such as traveling by
car, being indoors only, vacuuming/cooking/etc, wherever possible.

Receptor models will be developed to determine and quantify the sources of PM and air toxics in
DEARS samples (community, outdoor home, indoor, and personal) at the central site, homes
near roadways (< 50 m), and homes farther away from roadways (> 150 m). In addition, the
relationship (correlation and attenuation) between central site source contribution estimates and
personal exposure to the central site source contributions will be determined.

6.0 QUALITY ASSURANCE

A Quality Assurance Project Plan (QAPP) will be prepared that describes quality assurance
goals and the methods that will be used to meet these goals. The plan will address both the
overall DEARS analysis plan as well as the individual sample collection and analysis
procedures that will provide the data. Critical elements of the Quality Assurance Project Plan
include (U.S. NAMS 005/80):

•	Project description

•	Project organization

•	QA objectives

•	Sampling procedures

•	Sample custody

•	Calibration frequency and procedures

•	Analytical procedures

•	Data reduction, validation and reporting

•	Internal quality control checks

•	Performance and system audits

•	Preventative maintenance

•	Routine procedures to assess data quality

•	Corrective actions

•	QA reports to management

•	Standard Operating Protocols (sops) for sample collection and analysis

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The Quality Assurance Plan prepared for the RTP PM Exposure Panel Studies will serve as the
basis for this plan. Selected components will be revised and/or updated including the project
description, sampling populations, cited instruments, data quality objectives, analytical
procedures and the calibration frequency of listed equipment. The plan will be reviewed and
revised as needed before measurements are performed. All work will be conducted as described
in the approved QA Plan.

In addition to developing an approved QA Plan, NERL will be responsible for demonstrating
compliance to the plan. Field and laboratory audits will be conducted during selected portions of
the work. For these audits, NERL QA specialists will review and observe EPA and contract field
staff during sample collection, calibration of test equipment, documentation of sampling/field
efforts, and data reduction and reporting, etc. Formal audit reports will be prepared and released
to the NERL-HEASD Quality Assurance Director. A QA report will be prepared by the NERL
QA staff at the end of the project

7.0	MANAGEMENT

7.1	Schedule

Table 7-1 reports the anticipated timeline of the study and pertinent events. In general, field
activities are anticipated to begin in the summer of 2004. This will be followed by a winter
monitoring program in early 2005 and then a repetition of summer and winter sampling for two
additional years. Laboratory and statistical analysis of seasonal samples/data will be performed
throughout the initial three years of the field study and continuing until goals 1-6 have been
achieved (approximately the fall of 2007). Databases will be expanded as additional validated
data becomes available.

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Table 7-1 Anticipated DEARS Timeline

Task		Projected Date

Complete study design peer review

July 2003

Receive OMB approval for data collection

April 2004

Receive IRB and EPA study approval
involving human subjects

May 2004

Start first summer field monitoring session

June 2004

Start first winter field monitoring session

January 2005

Begin development of validated datasets

May 2005

Perform initial analyses

June 2005

Start second summer field monitoring session

June 2005

Start second winter field monitoring session

January 2006

Start third summer field monitoring session

June 2006

Start third winter field monitoring session

January 2007

Complete all laboratory analyses

December 2007

Validate all data and datasets

December 2008

Complete all analyses and report summary
findings

December 2009

8.0 REFERENCES

Bagley, S. T., Gratz, L. D., Johnson, J. H., and McDonald J. F. 1998. Effects of an oxidation
catalytic converter and a biodiesel fuel on the chemical, mutagenic, and particle size
characteristics of emissions from a diesel engine. Environ. Sci. Technol. 32: 1183-1191.

Bortnick, S., and Hafner, H., 2001. Air toxics monitoring data: analyses and network design
recommendations. Technical Report. Batelle Memorial Institute, OH & Sonoma
Technology Inc., CA

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APPENDIX A: SOURCE-RELATED EXPOSURE FINDINGS

Janssen et al. (2001) studied the exposure to traffic related air pollution of children attending
schools near motorways. 24 schools within 400 m of motorways in the Netherlands were
monitored for PM2.5, N02, EC (reflectance) and benzene. Particles and benzene were averaged
over school hours only during one week. N02 was averaged over a full week. Each school was
measured 5-10 times over 16 weeks. Main findings were that fine particles and EC significantly
increased with increasing truck traffic and significantly decreased with increasing distance from
the motorway. N02 significantly increased with car traffic. Soot, benzene, and N02 had a 2.5-
fold range, but PM2.5 had only about 1.5-fold.

Roorda-Knape et al. (1998) measured air pollution from traffic in city districts near major
motorways. Ambient and indoor measurements were made in 12 schools in six cities. Outdoor
measurements of PM10, PM2.5, black smoke (BS), benzene, and N02 were made in two cities;
N02 alone in four more cities. Indoor measurements included weekly averages of PMi0,
benzene, and NO2. The main findings from this study were that EC and NO2 declined with
increasing distance from roadways up to 100-150 m. No distance effect was noted for particles or
benzene. PM10 outdoors was 32 ng/m3 at the two cities, but 50-165 ng/m3 indoors at the 12
schools. BS was 12-15 ng/m3 outdoors, 5-20 ng/m3 indoors.

Van Vliet et al (1997) studied motor vehicle exhaust and chronic respiratory symptoms in
children living near freeways. Ambient and indoor measurements of PMi0, PM2.5, black smoke,
and NO2 were made in 13 schools within 1000m of major freeways in South Holland. Lung
function and chronic respiratory symptoms were ascertained for 1500 children 7-12 years old
(1068 usable questionnaires). Home addresses were plotted as distance from roadway. The main
result was that girls (but not boys) living less than 100m from the roadways had higher
symptoms.

Kingham et al., (2000) studied spatial variations in the concentrations of traffic-related pollutants
in indoor and outdoor air in Huddersfield, England. Outdoor and indoor measurements were
made in paired homes <50 m and >50 m from roadways. 24-h daily averages of PM10, PM2.5,
EC by reflectance, benzene, benzo-a-pyrene (Bap) and other PAHs were made over two 2-week
periods. A total of 49 homes (23 near, 26 far) took part. No spatial gradients were apparent for
any pollutants.

Fischer et al., (2000) studied traffic-related differences in outdoor and indoor concentrations of
particles and volatile organic compounds in Amsterdam. Outdoor and indoor measurements were
made in paired homes: major streets vs. quiet streets. 24-h daily averages of PMi0, PM2.5, EC by
reflectance, 15 PAHs, and 8 VOCs were collected in 36 homes (18 & 18). Results indicated that
outdoor particle levels were only slightly (15-20%) higher in the homes near major streets, but
100% increases for BaP, total PAH, EC, benzene, and total VOC were documented. Indoor
levels also increased for the latter except for the VOCs.

Kousa et al., (2002) studied associations between ambient fixed site, residential outdoor, indoor,
workplace, and personal exposures to PM2.5 in four European cities in the EXPOLIS study.
Central-site, back yard, indoor, workplace, and personal measurements were made using a

A-l


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probability sample of working-age populations (N = 50 in most cities, 200 in Helsinki) in six
cities. Measurements were made using 48-h personal PM2.5 (PEMs) with two filters, one for
work and the other for non-work time; 48-h microenvironmental monitors (MEMs) at home,
outdoors, and workplaces, programmed to run during expected times at home and work; and 24-
h monitors at central sites. Results included low personal-ambient correlations (r = 0.25-0.37) for
the full Helsinki population; slightly higher personal-ambient correlations (r = 0.34-0.47) for
non-ETS exposed population. Indoor-ambient correlations (r = 0.22-0.41) were also low. High
correlations were noted between residential outdoor and central-site measurements.

Edwards et al., (2001) reported on VOC concentrations in in EXPOLIS in Helsinki. Outdoor,
indoor, workplace, and personal 48-h VOC measurements were made for 183 persons in a
probability sample of working-age populations. Results showed that 21 of 22 compounds had
indoor mean concentrations 2-7 times higher than concurrent outdoor levels. ETS-exposed
subjects (40% of total) had twice the BTEX and styrene levels of non-ETS exposed.

Edwards et al. (2001) extended the previous study to identify VOC sources for a subset (N=l 11)
of the non-ETS-exposed participants. Four factors were identified, associated with traffic
emissions, cleaning products, emissions from trees (a-pinene), and long-range transport.

Hoek et al., (2002) studied the spatial variability of fine particle concentrations in three European
areas PM2.5 and EC were monitored at 40 sites for four 14-day averages. Continuous
monitoring at a central site in each of three areas was carried out to remove bias from temporal
variation. Annual averages ranged from 11-20 |ag/m3 in Munich, 8-16 |ag/m3 in Stockholm, and
14-26 ng/m3 in the Netherlands. Traffic-related sites were 17-18% higher than background sites
in PM2.5 but 31-55% higher in EC.

Ruuskanen et al. (2001) studied concentrations of ultrafine, fine and PM2.5 particles in three
European cities. One site in each of three cities in the Netherlands, Germany, and Finland Was
monitored during winter only. 10-minute average measurements were takenof ultrafines, total
particle number, PM2.5, and EC.PM2.5 was poorly correlated with ultrafines, but well correlated
with accumulation mode number concentration. Principle Components Analysis (PCA)
indicated two components, one associated with number, the other with mass.

Houthuijs et al (2001) measured 24-hour average concentrations of PM10 and PM2.5 in 24 study
areas in six countries in Central and Eastern Europe. Samples were collected every sixth day.
Additional sampling at one or two urban background sties within each area took place for one or
two months. PM2.5 increased significantly in the heating season (from 18-45 |ag/m3) with no
increase in coarse particles. Within-area spatial variation was much smaller than between-area
variation (but the sample size for within-area comparisons was limited).

Van der Zee et al., (1998) characterized particulate air pollution in urban and non-urban areas in
the Netherlands Daily measurements of PMi0, EC, sulfate, nitrate, ammonium, acidity, elements
took place overthree consecutive winters in three urban and three non-urban areas. PM2.5 was
added in the third year. PMi0 and EC were 13-19% higher in urban areas; PM2.5 was not higher.
All elements significantly higher except for Si.

A-2


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Hoek et al. (2002) found that cardiopulmonary mortality was associated with living near a major
road for a Dutch cohort.

Delfino (2002) published a review of epidemiological studies of the relationship between asthma
prevalence or morbidity and traffic-related exposures. Delfino concluded that his review "gives
the overall impression that asthma, related respiratory symptoms, lung function deficits, and
atopy are higher among people living near busy traffic."

Other studies including Shao et al. (2002), Buckeridge et al. (2002), and Brauer et al. (2002)
indicate the potential for adverse respiratory outcomes among populations living near roadways.
Pearson et al. (2000) associated distance-weighted traffic counts in Colorado on the nearest road
to children's residence with increased odds of childhood cancer, including leukemia using a
case-control design, although other studies have found no associations (Langholz et al., 2002;
Reynolds et al., 2002).

A-3


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Appendix B: Power calculation

One of the primary objectives of the Detroit study is to evaluate and describe the physical and
chemical factors that determine the impact of various ambient sources on outdoor and indoor
residential concentrations and the spatial variability of these concentrations in relation to the
source locations. As detailed in section 5.2.1, this can be accomplished based on a simple linear
relationship in which residential (outdoor or indoor) concentrations (i.e., the dependent y-
variable) are regressed onto the ambient concentrations (i.e., the independent x-variable).| The
strength of such a linear relationship is measured by R2 = SSR/SST ,the coefficient of
determination, where SSR is sum of squares due to regression, and SST is the total sum of
squares in the y-variable corrected for mean. R2 can be interpreted as the amount of variation in
the y-variable accounted for by regression onto the x-variable. For a simple linear regression, R2
is the square of the correlation between xy pairs. Regressions will be calculated for each cross-
sectional group of source and location categories as discussed in section 3.3.2 and shown in
Table 3-8 in order to compare the effects of different point sources, mobile sources, and
locations on the slopes and intercepts.

Our study calls for a sample size of 5 days/season/ household. The study will be conducted over
3 years; two seasons (winter and summer) per year. Approximately 40 households will be chosen
each year giving a total of N = 3*40*2*5=1200 measurements. However, since regressions will
calculated for groups of different sample sizes Figure A-l shows power curves relating N, R2,
and power at the alpha = 0.05 significance level. Each curve shows the probability (i.e., power)
of detecting R2 > 0 for different sample sizes. Each value of R2 represents a different alternative
to the null hypothesis of zero. These curves were derived using the noncentral F-distribution in
SAS and allowing N to range from 5 to 30 and R2 from zero to 1.0 at the alpha=0.05 significance
level. For example, for a single household with N = 5 days per season, the R2 for a simple linear
regression needs to be at least 0.78 to have a high probability (0.80) of being significantly
different from zero at the alpha = .05 significance level. If the results from two seasons within a
year can be pooled (N = 10), the R2 can be as low as 0.50 and still be significant. Rejecting a
null hypothesis of a zero slope is equivalent to rejecting a null hypothesis of R2 = 0. Pooling
measurements across residences within source groups may provide a larger sample size on which
to base regression estimates, however the calculated R2 will not necessarily increase with more
samples, but rather tends to settle down around the population value.

Because of the inherent relationship between linear regression, correlation, and R2, the relative
difference between independent regression slopes can be detected at a specified probability level
(power) using the appropriate sample sizes and R2 values. For example, Table A-2 shows that for
a correlation of r = 0.90 (R2 = .81), a sample size of N = 45 per group is required in order to
have a probability of at least .78 of detecting a relative difference of 5 = ± 20% (± 0.20) between
estimated slopes at the alpha = .05 significance level. The highlighted
probabilities correspond to combinations of sample sizes (nl,n2) and R2 values (rsl, rs2) that
provide a better than 50:50 chance of detecting a specified relative difference at the alpha = .05
significance level. A comparison of intercepts is valid only if the slopes are equal.

A-4


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Figure A-l

A-5


-------
Table A-2. Power of t-test to detect differences of magnitude D(%) between two slopes for two
different source groups: under the null hypothesis of no difference and an alpha=.05 significance
level

	 n1=40 n2=80	



O)
T

II

CO
£_

O)

ii

CO
£_

O)

ii

CO
£_

rs1=.64

rs1=.64

rs1=.64

CO

ll

CO
£_

CO

ii

CO
£_

CO

ii

CO
£_

D(%)

rs2=.49

rs2=.64

CO
II

OJ
CO
£_

rs2=.49

rs2=.64

CO

ii

OJ
CO
£_

rs2=.49

rs2=.64

CO
II

OJ
CO
£_

10

0.07

0.07

0.08

0.08

0.09

0.10

0.10

0.13

0.16

20

0.14

0.15

0.16

0.19

0.22

0.25

0.27

0.35

0.45

30

0.23

0.25

0.27

0.34

0.39

0.44

0.50

0.62

0.74

40

0.34

0.37

0.39

0.50

0.56

0.61

0.71

0.82

0.90

50

0.45

0.48

0.50

0.64

0.70

0.75

0.86

0.93

0.97







	 n-j

—A c, n9-^n



















4 3 11 d. OU













O)
T

II

CO
£_

O)
T

II

CO
£_

O)

ii

CO
£_

rs1=.64

rs1=.64

rs1=.64

rs1 = .81

rs1 = .81

rs1 = .81

D(%)

rs2=.49

rs2=.64

CO
II

OJ
CO
£_

rs2=.49

rs2=.64

CO

ii

OJ
CO
£_

rs2=.49

rs2=.64

CO

ii

OJ
CO
£_

10

0.06

0.07

0.07

0.06

0.08

0.09

0.07

0.09

0.13

20

0.11

0.13

0.16

0.13

0.17

0.23

0.15

0.22

0.35

30

0.19

0.23

0.27

0.23

0.31

0.41

0.29

0.42

0.62

40

0.28

0.34

0.40

0.36

0.47

0.58

0.45

0.63

0.83

50

0.38

0.45

0.52

0.49

0.61

0.73

0.61

0.79

0.94

n1=45 n2=45

rs1=.49
D(%) rs2=.49

rs1=.49
rs2=.64

rs1=.49
rs2=.81

rs1=.64
rs2=.49

rs1=.64
rs2=.64

rs1=.64
rs2=.81

rs1 = .81
rs2=.49

rs1 = .81
rs2=.64

rs1 = .81
rs2=.81

10
20
30
40
50

0.06
0.13
0.22
0.32
0.43

0.07
0.15
0.25
0.37
0.49

0.08
0.17
0.29
0.41
0.53

0.07
0.16
0.29
0.43
0.58

0.09
0.20
0.36
0.53
0.68

0.10
0.25
0.44
0.62
0.76

0.08
0.20
0.38
0.57
0.74

0.11
0.28
0.52
0.74
0.88

0.15
0.41
0.70
0.88
0.96

A-6


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Appendix C: Source Apportionment
Sampling Designs

The PTEAM study was the first large-scale probability-based study of personal exposure to
particles conducted in fall of 1991 in Riverside, California (Ozkaynak et al., 1994a - b). Personal
exposure to PMi0 was measured for 178 participants selected for study based on socioeconomic
stratification, after examining their screening interviews. Sampling continued during 49 days:
from September 22 to November 9, 1991. Each participant wore personal exposure monitors
(PEM) for two consecutive 12-hour periods. The PEM design is discussed in detail by Ozkaynak
et al. (1996). PMi0 and PM2.5 samples were also collected with stationary indoor monitors (SIM)
and stationary ambient monitors (SAM) at each home for every observation period. This
resulted in 10 samples per household (day and night samples from PEMio, SIMio, SIM2.5, SAM10
and SAM2.5). A central outdoor site was maintained during the entire period, where PM was
measured by two high-volume PMi0 samplers , two dichotomous PMi0 and PM2.5 samplers, one
PEM, and one SAM. Following each of the two 12 hour monitoring periods, the participants
answered an interviewer-administered questionnaire concerning their activities that might
involve the exposure to increased particle level (nearby smoking, cooking, gardening, etc.) and
locations during monitoring time. All of the filters were weighed on-site in a van with controlled
temperature, humidity and protection from vibration. Elemental concentrations were determined
by X-ray fluorescence (XRF). Fourteen elements (Al, Si, S, CI, K, Ca, Ti, Mn, Fe, Cu, Zn, Sr,
Br, Pb) were present in measurable quantities on a majority of the filters.

In a recent study by EPA, Williams et al. (2000a, 2000b) evaluated the relationship between
PM2.5 mass measured at a stationary outdoor community location in central Baltimore county
(Towson, Maryland) and personal exposure observations from July 26 to August 22, 1998 at an
18-story retirement facility 11 km away. The study was designed to minimize the
microenvironmental activities that were previously shown to contribute to elevated exposures,
and to evaluate PM2.5 exposure of susceptible populations that might have higher risk factors
than other segments of the population.

Daily personal and every other day apartment PM2.5 samples were collected on 37 mm Teflon
filter media using inertial impactor Personal Exposure Monitors (PEMs) manufactured by MSP
Inc., sampling at 2 liters per minute. The PEM samplers were refurbished daily and randomly
reassigned to avoid any potential bias. PEM samplers were also collocated with a Versatile Air
Pollutant Sampler (VAPS) at the indoor, outdoor, and central community sites (Clifton Park Golf
Course). The VAPS is a modified dichotomus sampler which collects PM2.5 on both Teflon and
quartz filter media (15 LPM), and also collects concentrated coarse (PM8.o - PM2.5) particulate
matter on aNucleopore® filter (2 LPM) (Pinto et al., 1998). Outdoor sampling at the 18-story
retirement facility was conducted with another VAPS on the rooftop of an attached three-story
retirement facility. Centralized indoor sampling was conducted with a third VAPS in an
unoccupied fifth floor apartment of the retirement facility. The windows in this location
remained closed, but the apartment door was kept open to the facility's central hallway.

A-7


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The Teflon filters from the VAPS and PEM samplers were analyzed using XRF. The VAPS
Teflon and Nylon filters were subsequently extracted in deionized water and analyzed for major
ions (NH, SO, and NO) and Na+ by ion chromatography (IC) (Stevens et al., 1978). The NH, SO,
and Na+ determinations were made from the Teflon filter extracts and the NO results are the sum
of the Teflon and Nylon filter extract determinations. The VAPS quartz filters were manually
split half was used for organic carbon (OC) and elemental carbon (EC) analysis by thermal
optical analysis (TOA) (Birch and Cary, 1996).

Receptor Modeling Approaches

It is suggested by the following, that there is no single model that will be generally applicable to
all situations. For the PTEAM data, a three-way analysis was employed. The model is written
as

p

xijk=Y,aihbjhckh+eijk	(i)

h=1

In this equation, a,i, is the hth source contribution for each ith sampling interval, bjh contains the
jth concentration in the hth source profile, and Ckh indicates the kth sample type (community,
indoor, or outdoor) such that the product of a,i, and Ckh provides the source contributions for each
sample within the data set. This equation can be rewritten in matrix form as

X = A • B • C + E	(2)

The three modes used in the analysis were (1) 18 elemental concentrations, (2) 303 samples, and
(3) sample type (personal PMio, indoor PMio, outdoor PMio, indoor PM2.5, outdoor PM2.5). This
analysis provided the factor profiles, their contributions, and the distribution of each source
between personal, indoor, outdoor fine and coarse (PMio - PM2.5) PM. Fit to the data was
reasonably good and the individual factors could be readily interpreted.

For the Baltimore Exposure Panel Study (BPMEES), this three-way model was used to analyze
the fixed site monitors at the community site, outside and inside the residence facility (Hopke et
al., 2002). In this case, the indoor sources could be readily distinguished from the clearly
outdoor sources. It was also possible to estimate the fraction of indoor particulate matter
concentration that was largely due to the presence of outdoor particles in the indoor environment.

However, in the case of the personal and apartment particulate matter concentrations measured in
the BPMEES (Hopke et al., 2003), it was found that a three-way model (Equation 1) did not
provide a good fit to these data. After further exploration, a more complex model was developed
that did provide an appropriate fit to the data and interpretable factors. In this new model, two
different categories of factors are defined. In one category are external factors that contribute to
all four types of environments (indoor, outdoor, personal, and apartment) and have the same
composition profiles for all of the types of samples. The other category consists of internal
factors that contribute mass to only the personal and apartment samples and thus, attempt to
represent sources specific to this subset of samples. This model is then used to fit all of the
different types of samples. A number of factors (N) are used for fitting all samples, while P
factors are used only to fit the personal and apartment samples. The set of P factors forms an
ordinary 2-way factor model. The N factors of the first set must fit the indoor and outdoor data,

A-8


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and thus they form a basis set for them. This basis set is used for fitting the personal and
apartment data, together with the additional 2-way model. The equations of the model are
relatively simple:

N

N+P

(/ = 1,	,15, t = pers/apt data)

p=1	p=N+\

p=N+\

(3)

N

(/ = 16, t = indoor/outdoor data)

where aiPdt is the contribution of the pth source to sample type t collected on day d and bjp is the
concentration of the jth chemical species in particulate matter from the pth source. Such a model
can be fitted using the multilinear engine (ME) (Paatero, 1999).

The three modes used in the analysis were (1) 42 elemental concentrations plus unknown mass
(PM2.5 - sum of the oxides of the crustal elements - sulfur as ammonium sulfate), (2) 391 PEM
samples, and (3) sample type (personal PM25, apartment PM25, indoor PM25, outdoor PM2 5).
This analysis provided the factor profiles, their contributions, and the distribution of each source
for both external and internal factors between personal, apartment samples. In addition, the
external source contributions to outdoor and indoor were reported. In general, a reasonable
understanding of the particle sources was obtained and specific unusual samples could be readily
identified by evaluating each subjects external and internal source contribution estimates.

From these results, it appears that there is no single model that can be routinely applied to the
range of possible measurement plans. The model must be structured based on the measurement
plan. If a standard protocol were developed that was employed in repeated studies, it may be
possible to develop a model that would be applicable to all such studies.

Interpretation of Results

For the PTEAM data, reasonable fits could be obtained using the three-way model in equation
(2) where the A matrix provides the series of source contributions for each individual, B contains
the source profiles, and C indicates the sample type such that the product of A and C provides
the source contributions for each sample within the data set. Using this approach, major particle
sources of personal PMio exposure in Southern California in Fall 1991 were resuspension of the
soil particles, generation of the particles by personal activities, penetration of fine particles from
the outdoors, such as emissions from oil combustion or nonferrous metal operations and motor
vehicle exhaust. Sources of coarse particles such as sea-salt or ambient soil did not directly
influence indoor air conditions and personal exposure to PMi0. Sources of fine particles such as
secondary sulfate, oil combustion, nonferrous metal operations and motor vehicle exhaust had
similar impacts on outdoor, indoor and personal PMi0. In addition, good agreement was
obtained between information about daily personal activities and values of factor scores.
Smoking, indoor cooking, vacuuming resulted in elevated "personal activities", "indoor soil",
and "resuspended indoor soil" factor scores, and did not affect "secondary sulfate" and
"nonferrous metal operations and motor vehicle exhaust."

From the results of the BPMEES data analysis (Hopke el al., 2002), it appears that there are
severe limits to how much interpretation can be applied to the results for individual personal or
apartment samples. Because of the uncertainties in input data, the limited number of samples,

A-9


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and the apparent unique behavior of specific individuals that cannot be reflected in a factor
analysis, it is necessary to recognize the limitations on how much information can be gleaned on
all of the sources leading to the exposure of individuals to airborne particulate matter. There are
difficult decisions to be made by the data analyst as to whether or not to retain unusual values in
the analysis since in some cases, such as person 5 in the BPMEES data base, it is clear that
sources are producing exposure to the specific individual and apartment that are unrelated to any
other participant in the study.

A-10


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Appendix D: Survey Questionnaires

•a EPA::

Daily follow-up Questionnaire

(technician administered)

Participant ID	Start Date (yesterday' s date)

~~~~ ~~/~~/~~

AABCD MM DD YY

A = Participant ID
B = Cohort
C = Cycle

This form is a hard copy version of a computer driven questionnaire. The overall
format and layout of the computer questionnaire may differ significantly, but this hard copy
version provides an accurate representation of the questions and overall extent of the
information we wish to capture in the follow-up interview with the participant. The tables in
the computer version can capture many more events/occurrences of an activity than is
indicated in this hard copy version. The use of'24 hours' throughout the questionnaire refers
to the previous monitoring period in its entirety.

A-ll


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1. Did you or anyone smoke (cigarette, cigar or pipe) around you within the last 24
hours at any location? Y ~ / N ~

If yes, please indicate in the following table where and when smoking occurred. If you
smoked or your exposure to smoke occurred repeatedly over time in one location, please only
indicate a single start time and duration for the entire time you smoked or were exposed to
smoke in each location.

Location1

Time
(start)

Duration
(minutes)

Comments

























1 - (IH) Indoors at Home, (10) Indoors at ot

ler, (O) Outdoors, (C) Car or other vehicle

2. Did you or anyone smoke (cigarette, cigar or pipe) inside your home within the last 24
hours? Y ~ / N ~

If yes, please indicate in the following table when smoking occurred inside your home. If
smoking occurred repeatedly over time, please only indicate a single start time and duration for
the entire time you or someone else smoked inside your home.

Time
(start)

Duration
(minutes)

Comments



















3. Did you cook or were you around when someone else was cooking during the
last 24 hours? Y ~ / N ~

If yes, please fill out the following table.

A-12


-------
Your
Location1

Cooker
Type2

Type of
cooking3

Time
(start)

Duration
(minutes)

Smoke
Produced4

Exhaust
Fan5

Comments

















































1 - (IH) Indoors at Home, (10) Indoors at ot

ler, (O)utdoors, (C)ar or other vehic

e

2	- (S) Stove, (M) Microwave, (O) Oven, (G) Grill

3	- (FG) Frying or grilling, (BB) Baking or broiling, (TO) Toasting, (BO) Boiling, (OT) Other,
please specify

4	- Was anything burned while cooking that produced visible smoke? (Y, N, Don't Know)

5	- Was an exhaust fan used that was vented outdoors? (Y, N, Don't Know)

4. Were you around burning candles or incense at any location during the
last 24 hours? Y ~ / N ~

If yes, please indicate in the following table your location and when you were around

burning candles or incense.

Your
Location1

Time
(start)

Duration
(minutes)

Type2

Metal
Wick3

Comments





































1	- (IH) Indoors at Home, (10) Indoors at other, (O)utdoors, (C)ar or other vehicle

2	- (C) Candles or (I) Incense

3	- Applies to candles only. Did the candle have a metal wick?

5. Did you use a humidifier in your home in the last 24 hours? Y ~ / N ~

If yes, please fill out the following table.

Time
(start)

Duration
(minutes)

Humidifier
Type1

Water
Type2

Additives3

Comments





































1	- (E) Evaporative, (U) Ultrasonic cool-mist, (H) Heated, (O) Other

2	- (T) Tap, (D) Distilled, (B) Bottled, (O) Other

3	- Specify additives including mentholatum, etc.

A-13


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6.	Was your primary heater (furnace, etc.) used in your home during the

last 24 hours?	YD N ~

7.	Were any other heating devices used in your home during the last

24 hours? YD N ~

If yes, please indicate in the following table when and what type of device was used.

Time
(start)

Duration
(minutes)

Device
Type1

Smoke or fuel
smelled?

Door open2

Comments





































1	- (WF) Wood burning fireplace, (GF) Gas logs fireplace, (WS) Wood burning stove, (KE)
Kerosene space heater, (O) Other, please specify.

2	- Applies to a wood stove only. Other than to add wood, was the door left open on the wood
stove while it was in operation?

8.	Was an air conditioner run during the last 24 hours in your home?	Y ~ ND

9.	Were any windows open in your home in the last 24 hours? Y ~ ND

If yes, please indicate in the following table when, the number of windows and how
many were open wider than 6 inches.

Time
(start)

Duration
(minutes)

# Windows
Open

# Open >
6"

Comments































10. Were any exterior doors left open for more than five minutes or were screen doors used for
ventilation in your home during the the last 24 hours?	YD N ~

If yes, please indicate in the following table when exterior doors were open.

Time
(start)

Duration
(minutes)

Comments



















A-14


-------
11. Was an air cleaner or air filter used in your home in the last 24 hours? Y ~ / N ~

If yes, please indicate in the following table when and the type of air filter/cleaner.

Time
(start)

Duration
(minutes)

Type1

Comments

























1 - (H) HEPA filter, (Z) Ozonator, (E) Electrostatic precipitator, (O) Other, please specify

12. Were housecleaning chores performed by you or someone else in your home during the last
24 hours?	YD/ND

place.

If yes, please indicate in the following table when and the type of cleaning that took

Time
(start)

Duration
(minutes)

Type of
cleaning1

Comments

































1 - (V) Vacuuming, (S) Sweeping, (D) Dusting, (O) Other, please specify.

13. Were cleaning products used in your home within the last 24 hours? Y ~ / N ~

If yes, please indicate in the following table when and the cleaning product(s) used.
Please do not include bleach, ammonia based cleaners (e.g. Windex), vinegar, baking soda,
dishwashing detergent, laundry detergent

Time
(start)

Duration
(minutes)

Cleaning
Product

Comments

























A-15


-------
14. Were any of the following aerosol spray products used in the home within the last 24 hours?
Air freshener, spray perfume or cologne, hair spray, spray deodorant.

Time
(start)

Duration
(minutes)

Type1

Comments

























1 - (AF) Air freshner, (PC) Perfume or cologne, (HS) Hair spray, (SD) Spray deodorant, (OT)
Other, please specify.

15. Were any petroleum based solvents, paints or glues used in or around your home during the
last 24 hours? Petroleum based solvents include paint thinner, paint stripper, etc. Paints may
include oil based and latex or acrylic paint. Y ~ / N ~

If yes, please indicate type of solvent or paint used during the last 24 hours	

16.	Were any dry-cleaned items (clothes, etc.) brought into your home during the last 24 hours?
Y ~ /N ~

17.	Did you smell smoke or any other unusual chemical smells in or around your home within
the last 24 hours that you have not already identified? Y ~ / N ~

If yes, please indicate in the following table when and the type and source of odor, if
known.

Location1

Time
(start)

Duration
(minutes)

Type and
source of odor

Comments































1 - (I) Indoors, (O) Outdoors

18. Were household or lawn chemicals used around your home in the last
24 hours? YD N ~

If yes, please indicate in the following table where, when and the type of chemical(s)
used.

A-16


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Location1

Time
(start)

Duration
(minutes)

Type2

Comments































1	- (I) Indoor, (O) Outdoors

2	- (H) Herbicides, (P) Pesticides, (O) Other, please specify

19. Were lawn mowers and/or other small engines used around your home in the last 24 hours?
YD N ~

If yes, please indicate in the following table when and the type used.

Time
(start)

Duration
(minutes)

Type1

Comments

























1 - (L) Lawn mower, (W) Weed eater, (B) Blower, (O) Other, please specify.

20. Did you drive or were you a passenger in a motor vehicle of any type within the last 24
hours?	YD N ~

If yes, please indicate in the following table when and what type of vehicle.

Time
(start)

Duration
(minutes)

Vehicle
Type1

Comments

























1 - (C) Car, (T) Truck, (B) Bus, (M) Motorcycle, (O) Other, please specify

21.	Did you put gas in a vehicle or were you in a vehicle while it was being refueled in the last
24 hours?	YD/ND

22.	If a garage is connected to the home, did anyone leave a vehicle or a small engine appliance
(e.g. lawnmower, weed-wacker, etc.) running in the garage longer than 30 seconds during the
last 24 hours?	Y ~ / N ~

23. If a garage is connected to the home, did anyone drive a vehicle into the garage after it was
running for more than 5 minutes? Y ~ / N ~

A-17


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If yes to either 22. or 23., please indicate in the following table when either of these
events occurred.

Time
(start)

Duration
(minutes)

Description1

Garage door2

Comments





















1	- Indicate whether (A) vehicle/other engine was running in garage longer than 30 seconds or
(B) a vehicle was driven into the garage after the engine was running for more than 5 minutes

2	- Indicate whether the garage door was (O) Open or (C) Closed during this activity. Was
the garage door closed just after a vehicle was driven into the garage?

A-18


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Neighborhood Source Survey Instructions

Purpose: To identify sources that may impact a participant's residence influencing indoor
concentrations of and exposure to PM/Air Toxics.

Identify any potential sources of PM and Air Toxics (ATs) within a 1 km radius in all directions
from the participant's residence. Potential sources are those that may alter concentrations of
PM/ATs at the residential or local level. Examples of source categories include (type of pollutant
indicated in parentheses):

Roads - the distance from the residence to the nearest roadway should be noted in the
comments column along with the GPS coordinates. Any other roadways within the 1 km
radius that could impact the residence should be noted as well. (PM, PAH, VOCs)
Construction - building (PM, diesel) and road (PM, diesel, asphalt/PAHs)

Diesel trucks/buses - warehouse/distribution hub, bus terminal, bus line/bus stop nearby,

railroad (PM, diesel)

Gas stations - indicate if diesel is served there? (BTEX, diesel vehicles)
Manufacturing/industry - type/description of facility (i.e. what is produced there -

need idea of what may be emitted in terms of PM/ATs)

Restaurant - meat cooking, wood burning, (PM, PAH)

Dry cleaner - solvents (VOCs)

Lumber yard - plywood, particle board, pressed wood (formaldehyde)

Automotive repair/body shop - sanding, solvents (PM, VOCs)

Janitorial services - cleaners/solvents (VOCs)

Photofinishing - solvents (chlorinated VOCs)

A-19


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Neighborhood Source Survey

Subject ID

Source Information

GPS Coordinates of Source

Source Description - Category

Comments

N
W

Source Information

GPS Coordinates of Source

Source Description - Category

Comments

N
W

Source Information

GPS Coordinates of Source

Source Description - Category

N
W

Comments

Draft

IS


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Participant Survey

Subject ID

1.	What is this person's sex?

G> Male

O Female

2.	What is this person's height in inches?

3. What is this person's weight in pounds?

4. What is this person's date of birth?





/





/









Note: Please answer BOTH Questions 5 and 6.

5. Is this person Spanish/Hispanic/Latino?

O No, not Spanish/Hispanic/Latino
O Yes, Mexican, Mexican Am., Chicano
O Yes, Puerto Rican
O Yes, Cuban

O Yes, other Spanish/Hispanic/Latino:

6. What is this person's race?

O White

O Black, African Am., or Negro
O American Indian or Alaska native
O Asian Indian
O Chinese
O Filipino
O Japanese
O Korean
O Vietnamese
O Native Hawaiian
O Guamanian or Chamorro
O Samoan
O Some other race:

7.	LAST WEEK, did this person do ANY work for

either pay or profit? Mark the "Y" oval even if the person
worked only 1 hour, or helped without pay in a family business or farm
for 15 hours or more, or was on active duty in the Armed Forces.

o

Skip to 12

8.	At what location did this person work LAST

WEEK? If this person worked at more than one location, print
where he or she worked most last week..

a. Address (Number and street name)

(if the exact address is not known, give a description of the location
such as the building name or the nearest street or intersection.)

b. Name of city, town, or post office

c. Name of U.S. state or foreign country

d. ZIP Code

9. How did this person usually get to work LAST

WEEK? If this person usually used more than one method of
transportation during the trip, mark the oval of the one used for most of
the distance.

O Car, truck, or van
O Bus or trolley bus
O Streetcar or trolleycar
O Subway or elevated
O Railroad
O Ferryboat
10. What time did this person usually leave
home to go work last week?

O Taxicab
O Motorcycle
O Bicycle
O Walked
O Worked at home
O Other method

AM/PM

Date





/





I









Draft


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Participant Survey

Subject ID

11.	Approximately how long doe it uauaslly take thiss person to commute one-way to work?

hr min

12.	Approximately how many hours each day are you away from home?

13.	Approximately how many hours each day do you spend outside?

14.	Do you have any hobbies that involve the use of solvents, paint, glue or textiles? CD CD

Draft


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Survey Approved

OMB Control No. 2080-0058

Approval Expires 7/31/2002

AM 6:00

: 15
:30
:45

7:00
: 15
:30
:45

8:00
:15
:30
:45

9:00
:15
:30
:45

10:00

:15
:30
:45

11:00
:15
:30
:45



In Transit

%

Outside at or
Near Home



i"

At Work away
from Home

Activity Description

Outside away
from Home

Indoors away
from Home

Smoker
Nearby
(minutes)

Cooking
(minutes)
Self Other

Subject ID

Start Date

MM D D Y Y

50268


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PM 12:00
:15
:30
:45

1:00

:15
:30
:45

2:00
:15
:30
:45

3:00
:15
:30
:45

4:00
: 15
:30
:45

5:00
:15
:30
:4S



In Transit

Outside at or
Near Home





At Work away
from Home

Outside away
from Home

Indoors away
from Home

Activity Description

Smoker
Nearby
(minutes)

Cooking
(minutes)
Self Other

Subject ID

Start Date

50307

** Same as previous page **


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PM 6:00
: 15
:30
:45

7:00
:15
:30
:45

8:00
: 15
:30
:45

9:00

:15
:30
:45

10:00
: 15
:30
:45

11:00

:15
:30
:45



In Jjfansit

Outside at or
Near Home



At Work away
from Home

Outside away
from Home

Activity Description

Indoors away
from Home

Smoker
Nearby
(minutes)

Cooking
(minutes)
Self Other

Subject ID

Start Date

50327

/

** Same as previous page **


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AM 12:00
1:00
2:00
3:00
4:00
5:00

6:00
:15
:30
:45

7:00
:15
:30
:45

8:00

:15
:30
:45

9:00
: 15
:30
:45



In Transit

Outside at or
Near Home



#;

'4 i- /

At Work away
from Home

Outside away
from Home



Activity Description

Indoors away
from Home

Smoker
Nearby
(minutes)

Cooking
(minutes)
Self Other

** Resume 15 min. intervals **

Subject ID

Start Date

/

50346

[53

** Same as previous page **


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Home and Vehicle Characteristics

GPS Coordinates of Residence

















N







.









1. Which best describes this building? Include all apartments, flats, etc., even if vacant.

O A mobile home	O A building with 2 or more apartments

O A one-family house detached from any other house O Boat, RV, van, etc.

O A one-family house attached to one or more houses O Other, please specify:

2.	Approximate age of building (years):

3.	How many people usually reside in this home?

4.	How many children (<18 years old) usually reside in this home?

4. a. What are the ages of the children in this home?

3.	What type of garage, if any, is there associated with the dwelling?

O None, detached, or separate carport O Attached O Underneath
3. a. Is this garage used for:

O Parking one car O Parking two cars O Parking more than two cars O Storage only
3. b. Indicate any small gasoline engine appliances stored in the garage:

O Lawnmower	CD Chain saw

O Weedwacker	O Other, please specify:

O Leaf blower	O None

3.c. Are gasoline or other petroleum based solvents stored in the garage?	CD

3.d. Is there a door leading from the garage into the dwelling? o ®

4.	Indicate below information about the vehicle(s) used for transportation:

Primary vehicle used for transportation:

Make	Model	Year

Make

Secondary vehicle usedfor transportation:
Model

Year

Draft

S3


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Residence Survey

Subject ID

Heating, Cooling and Ventilation Characteristics
1. How many separate central AC or window/wall units are in the home?

[ [ Central AC units

j	| Window/wall AC units

2. What are the heating sources in the home?

O Forced air gas	O Wood burning stove

O Forced air oil	O Fireplace, gas

O Forced air electric O Fireplace, wood
O Forced water, radiator O Gas space heater
O Heat pump	O Kerosene space heater

2.a. Indicate which heating source is NOT vented to the outside:

O Electric space heater
O Open stove/oven
O Other, please specify:

O None
O Forced air gas
O Forced air oil
O Forced air electric

O Heat pump
O Wood burning stove
O Fireplace, gas
O Fireplace, wood

O Kerosene space heater
O Electric space heater
O Open stove/oven
O Other, please specify:

O Forced water, radiator O Gas space heater

2.b. Indicate which heating source has an external fresh-air source:

O None
O Forced air gas
O Forced air oil
O Forced air electric

O Heat pump
O Wood burning stove
O Fireplace, gas
O Fireplace, wood

O Kerosene space heater
O Electric space heater
O Open stove/oven
O Other, please specify:

O Forced water, radiator O Gas space heater

3.	Is there a whole-house or attic fan?

4.	Are there storm windows? 0 CD

2

Draft

EH


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,-s?

Residence Survey

Subject ID

Cooking, Cleaning and Home Characteristics

1. What type of cooking fuel is used?

O Gas O Electric O Other, please specify:

2.	Is there an exhaust fan for the stove, range, oven, or elsewhere in the kitchen area? o CD

2.	a. How does this fan work?

O Kitchen exhaust vented outside O Other, please specify: 			

O Recirculation of indoor air O Don't know
O Charcoal filter

3.	Is there a clothes dryer? o cZ)

3.	a. Is the clothes dryer vented out of the dwelling? o CD

4.	Is there a continuously burning pilot light on a:

O Gas range O Oven O Clothes dryer O Water heater O Furnace

5.	Have freshly dry cleaned clothes been brought into the house during the last week? o CD

5.	a. If yes, how many days ago? 	

6.	Does anyone living here smoke inside your home? CD CD

If yes:

6.	a. How many persons living here smoke inside your home?

6. b. How many persons living here smoke cigarettes inside your home? 1 1

6.	c. How many persons living here smoke cigars or pipes inside your home?

7.	Does anyone use a humidifier in your home? o CD

7.	a. If yes, what type of humidifier?

O Ultrasonic	O Other, please specify:

O Evaporative	CD Don't know

Brand Name and Model —		—	

Draft

[63


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Recent Construction and Painting

1.	Have you painted in or around your home during the last 7 days or will you have any

painting done during the monitoring period? CD

2.	Do you have any new furniture that has been in your home less than 1 month? CD CD

3.	Have you had any new construction to your home during the last 6 months that involved

plywood or particle board, including cabinets, or any other pressed
wood products? O CD

4.	Have you had any new carpet installed in your home during the last 6 months? CD CD

5. Have you had any new linoleum installed in your home during the last 6 months? CD CD

Draft


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Residence Survey

Subject ID

Room Characteristics

Draw a floor plan of the home (each level, if applicable) in the space provided below. Please label the rooms using the following
format - KI (Kitchen), LR (Living Room), DR (Dining Room), FR (Family Room), BR# (Bed Room 1,2, etc.), BA# (Bathroom
1,2,etc.). Use the same names on the following page.

Draft


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Residence Survey

Subject ID

Use the same name for each room as used on the previous page.
Measurements of length and width are in feet.



Room

Length

Width

Floor
Coverage
%

Presence of
molds, mildew,
water damage

Dust factor for room
Clean Dusty

1

































©

©

©

©

©

©

o

o

2

































CD

©

©

©

O

o

o

©

3

































©



©

©

©

©

O

©

4

































©

©

©

©

©

O

O

©

5

































©

©

©

©

©

©

©

©

6

































©

©

©

©

©

©

O

©

7

































©

©

©

©

©

O

o

©

8

































©

©

©

©

©

©

o

O

9

































©

©

©

©

©

©

©

©

10

































©

©

©

©

©

O

©

o

11

































©

©

©

©

o

O

O

O

12

































©

©

©

©

©

O

O

©

13

































©

©i

©

©

O

©

©

©

14

































©

©

©

©

©

©

©

©

15

































©

©

o

o

©

©

©

o

6

Draft

® m


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