National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Appendix A: Equipment

& Supplies


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Base Kit

A Base Kit will be provided to the field crews for all sampling sites that they will go to. Some
items are sent in the base kit as extra supplies to be used as needed.

Base Kit Item	Quantity Protocol

Antibiotic Salve

1

Fish Plug

Aspirator bulb

1

Fish Plug

Beaker (3 L, Nalgene)

1

Water Chemistry

Centrifuge tube stand

1

Chlorophyll A

Centrifuge tubes (sterile, green screw-top, 50-mL) (10/pack)

1 pack

Chlorophyll A
Periphyton

Chlorophyll bottle (2 L, brown)

1

Chlorophyll A

Clinometert

1

Physical Habitat

Compasst

1

Physical Habitat

Delimiter - 12 cm2area

1

Periphyton

Densiometer - Convex spherical (modified with taped V)+

1

Physical Habitat

D-frame Kick Net (500 nm mesh, 52" handle) +

1

Benthics

Dry ice label (Class 9)*

5

Shipping

Electrical tape - roll*

1

General

FedEx labels, 5 sets of each in file folder (Tl, T2, T3, T5)*

1

Shipping

Filtration chamber adapter

3

Enterococci,
Chlorophyll A,
Periphyton

Filtration flask

1

Enterococci,
Chlorophyll A,
Periphyton

Filtration flask stopper (silicone, blue)

2

Enterococci,
Chlorophyll A,
Periphyton

Filtration unit (sterile 250 ml funnel, cap and filter holder) - spares

5

Enterococci,
Chlorophyll A,
Periphyton

Fish weigh scale, case and extra batteriest

1

Fish plug

Fish Voucher supplies (25 netting bags & 50 packaging ties/pack)

1 pack

Fish Voucher

Flagging tape (1 red, 1 blue) - rolls

2

Physical Habitat

Foil squares (aluminum, 5x5", 25/pack)*

2 packs

Chlorophyll A
Periphyton

Forceps (sterile, disposable) - spares

5

Enterococci,
Chlorophyll A,
Periphyton

Funnel (15 cm diameter)

1

Periphyton

Gloves (nitrile)*

1 box

General

Graduated cylinder (25 mL)

1

Periphyton

Graduated cylinder (250 mL)

1

Chlorophyll A,
Periphyton

HDPE bottle (1 L, white, wide-mouth) (extras)

12

Benthics, Fish
Vouchers

HDPE bottle (500 mL, white, wide-mouth) with graduations

1

Periphyton

Measuring tape (50 meter)+

1

Physical Habitat

Microcentrifuge tubes with glass beads (extras or for filter blanks)

5

Enterococci

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National Rivers and Streams Assessment 2018/19	Field Operations Manual

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Base Kit Item

Quantity Protocol

NRSA 2018-2019 Quick Reference Guide

1

General

NRSA 2018-2019 Field Operations Manual

1

General

Packing tape (extra rolls)*

2

Shipping

Packing tape dispenser (includes roll of tape)

1

Shipping

Petri dishes 60x15 (disposable, 20/pack)

1 pack

Filter Storage

Pipette (disposable, 2 mL)*

5

Periphyton

Rubbermaid Action Packer

1

General

Rubbermaid Roughneck tote (3 gallon)

1

General

Sieve bucket (500 nm)+

1

Benthics

Sounding rod (PVC 3 m , marked in 0.1 m increments)+

1

Physical Habitat

Sodium Thiosulfate Tablets ~30 tables (10 mL vial)*

1

Enterococci

Surveyor's telescoping leveling rod (rectangular, metric scale, 7.5m)+

1

Physical Habitat

Surveyor's level, CST Berger SAL 20+

1

Physical Habitat

Syringe (60 cc, with tip removed and tubing)*

1

Periphyton

Tape strips (25/pack)*

6 packs

General

Toothbrush (stiff-bristle, with handle bent at 90° angle)

1

Periphyton

Tripod for level+

1

Physical Habitat

Vacuum hand pump and clear plastic tubingt

1

Enterococci
Chlorophyll A
Periphyton

Wash bottles (1 L Nalgene) - 1 Dl, 1 river water

2

General

Wiffle golf ball

1

Discharge

Whatman 47 mm glass fiber GF/F 0.7 n filters (100/box)

1 box

Chlorophyll A,
Periphyton

Whatman 47 mm glass fiber GF/C 1.2 n filters (100/box)

1 box

Periphyton

47 mm polycarbonate 0.4 n filters (100/box)

1 box

Enterococci

*ltems may need to be replenished by field crews during field season
t Some items are sent in base kit as stock or extra supplies to be used as needed.

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Site Kit

A site kit will be provided to the field crews for each sampling site. Please submit an electronic
request form well in advance of field sampling. Kits must be requested at least two weeks
before sampling is to take place. Each site kit will include sample labels and will also include
necessary coolers and shipping supplies for all samples collected. Prior to sampling, inspect each
site kit to ensure all supplies are included. Some items may not be used at all sites and should
be held until the end of the field season and shipped back. These site kits include:

Site Kit Item

Quantity

Protocol

Centrifuge tubes (sterile green screw-top, 50-mL)

4

Chlorophyll A
Periphyton

Cooler Liner

1

Shipping

Cubitainer (4 L)

1

Water Chemistry

Fish Tissue Plug Kit
Sterile scalpel
Sterile biopsy punch
Sterile disposable forceps
20 mL acid washed glass scintillation vial

1
1
1
1
1

Fish Tissue Plugs

Forceps (sterile, disposable)

2

Enterococci
Chlorophyll A

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Field Operations Manual

Site Kit Item

Quantity

Protocol





Periphyton

HDPE bottle (1 L, white, wide-mouth)

2

Benthics

HDPE bottle (1 L, white, wide-mouth)

1

Fish Voucher

HDPE bottle (500 mL, white, round)

1

Algal Toxin

PETG bottle (500 mL, clear, square)

1

Algal Toxin

PETG bottle (125 mL, sterile, clear, square)

1

Periphyton
Metagenomics

PET bottle (250 mL, sterile, clear, square)

1

Enterococci

Phosphate buffered saline (sterile PBS)

1

Enterococci

Microcentrifuge tubes w/glass beads (in bubble bag & Ziploc bag)

2

Enterococci

Filtration unit (sterile 250 mL filter funnel, cap, and filter holder)

1

Enterococci
Chlorophyll A
Periphyton

Zip ties

2

Shipping

Whole Fish Tissue Kit

A whole fish tissue kit will be provided to the field crews for selected whole fish tissue sampling
sites (separately from site kits). Please submit an electronic request form well in advance of
field sampling. Kits must be requested at least two weeks before sampling is to take place. Prior
to sampling, inspect each site kit to ensure all supplies are included. These site kits include:

Whole Fish Tissue Kit Item	Quantity

Aluminum foil (solvent-rinsed and oven dried)

5 packs

Cable ties

24

Cooler

1

Dry Ice Information Sheet

1

Dry ice Shipping Label

1

FedEx airbill (Pre-addressed)

1

Frequently Asked Questions handout

1

Heavy-duty food grade polyethylene tubing

1 roll

Large plastic composite bag

1

Nitrile gloves

10

NRSA 2018/19 Fish Tissue Site List

1

Sampling Procedures handout

1

Tyvek tag

1

General Equipment

This equipment will need to be supplied by the field crew.

Crew Supplied Item

Quantity

Protocol

Barometer or elevation chart to use for calibration



Calibration

Batteries



General

Binoculars

1

Physical Habitat

Bleach (1-10 %, or bleach alternative)



Cleaning

Bucket

1

Benthics

Calibration cups and standards for multi-probe unit



Water Chemistry

Cell phone, 2-way radios, and/or walkie-talkies



General

Chest waders

2 pair

Benthics
Physical Habitat

Clipboard

2

General

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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Crew Supplied Item

Quantity

Protocol

Compass (Bearing - backpacking type) +

1

Physical Habitat

Current velocity meter, probe, and operating manual

1

Physical Habitat

Digital camera with extra memory card & battery

1

General

Dip Nets (non-conducting, mesh)

2

Fish Collection

Dry Ice



Storage/Shipping

Electrofishing equipment (boat, barge, and/or backpack units, including

1

Fish Collection

variable voltage pulsator unit, wiring cables, generator, electrodes, dip nets,





livewell and all safety equipment)





Ethanol (95%)



Sample

Field gear (e.g., protective clothing, sunscreen, insect repellent, hat,



General

water, food, backpack, cell phone)





Fisherman's vest (with lots of pockets and snap fittings)



General

Formalin (buffered, 10%)



Preserve Samples

GPS unit (with manual, reference card, extra battery)

1

Site Verification
Physical Habitat

Knife or scissors

1

General

Laser rangefinder (400 ft. distance range) +

1

Physical Habitat

Linesman gloves



Fish Collection

Measuring board (millimeter scale)



Fish Collection

Measuring tape with reel (50-100 m)

1

Physical Habitat

Meter stick (for bank angle measurements)

1

Physical Habitat

Multi parameter meter with pH, DO, temp, and conductivity probes)

1

Water Chemistry

Neutrally buoyant object (e.g., plastic golf ball, small rubber ball,



Physical Habitat

stick)





Pen, Pencils (#2, for data forms), Permanent marker (fine tip, for

1

General

labels)





Plastic Bags

2

Sample Storage

Plastic bucket (or similar container) with graduations (optional)

1

General

Portable Weir with 60° "V" notch (optional) and plastic sheeting

1

Discharge

Sampling permits and/or access permission letters (if required)



Site Evaluation

Scalpel for slitting open large fish before preservation



Fish Collection

Seine (10' or 20' x 6' minnow or bag seine - % inch mesh) (optional)



Fish Collection

Site maps/access instructions



Site Evaluation

Small spatula, spoon, or scoop to transfer sample

1

Benthics

Surveyor's flagging tape and/or pin flags



Physical Habitat

Top-set wading rod for use with current velocity meter

1

Physical Habitat

Watch with timer or stopwatch

1

Discharge

Watchmakers' forceps

1

Benthics

Water (deionized)



General

Wet ice



Sample Storage

f Item may be requested with Base Kit if needed

3

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National Rivers and Streams Assessment 2018/19
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Field Operations Manual

Boat Equipment

This is suggested boat equipment that would be supplied by the field crew.

Anchor (with line)

Boat horn
Boat plug (extra)

Bow/Stern lights
Emergency Tool kit
Gas Can

Hand Bilge pump
Lifejackets (1 per person)

Motor

Oars or Paddles
Sonar Unit
Spare Prop Shear Pin

Type IV PFD (Throwable Life Saving device)

Safety Equipment

Waders
Gloves

Sun-blocking Hat

Other appropriate field clothing

Safety glasses

First aid kits

Fire extinguishers

Blankets

Cellular/satellite phones or portable radios
Anti-bacterial soap
Clean water or ethyl alcohol
Medications


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Sample/Data Collection

This is a summary of the supplies that will be used in sample/data collection. Items are supplied
in the Base Kit, Site Kit or by the Crew as indicated.

Item	Quantity Protocol

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BASE KIT

Beaker (3 L, Nalgene)

1

Water Chemistry

Chlorophyll bottle (2 L, brown)

1

Chlorophyll A

Clinometer

1

Physical Habitat

Densiometer - Convex spherical (modified with taped V)

1

Physical Habitat

Delimiter - 12 cm2area

1

Periphyton

D-frame Kick Net (500 nm mesh, 52" handle)

1

Benthics

Electrical tape

1

General

Funnel (15 cm diameter)

1

Water Chemistry

Gloves (nitrile)



General

Graduated cylinder (25 mL)

1

Periphyton

Graduated cylinder (250 mL)

1

Chlorophyll A

HDPE bottle (500 mL, white, wide-mouth) with graduations

1

Periphyton

Sieve bucket (500 nm mesh)

1

Benthics

Sounding rod (PVC 3 m , marked in 0.1 m increments)

1

Physical Habitat

Surveyor's rod (rectangular, metric scale, 7.5m extended)

1

Physical Habitat

Syringe (60 cc, tip removed and tubing)

1

Periphyton

Toothbrush (stiff-bristle, with handle bent at 90° angle)

1

Periphyton

Wash bottle for stream water (1 L)

1

General

Wash bottle containing deionized water (1 L)

1

General

SITE
KIT

PET bottle (250 mL, sterile, clear, square)

1

Enterococci

Gloves (nitrile)

1 Pair

Enterococci

CREW SUPPLIED
(some items may be in base kit if requested)

Bearing compass (Backpacking type)

1

Physical Habitat

Binoculars

1

Physical Habitat

Buckets (plastic)

1

Benthics

Current velocity meter, probe, and operating manual

1

Physical Habitat

Dip nets (non-conducting, 1/4" mesh)

2

Fish Collection

Electrofishing equipment (boat, barge, and/or backpack
units, including variable voltage pulsator unit, wiring cables,
generator, electrodes, dip nets, and all safety equipment)

1

Fish Collection

Linesman gloves



Fish Collection

Livewell and/or buckets



Fish Collection

Measuring board (millimeter scale)



Fish Collection

Meter stick (for bank angle measurements)



Physical Habitat

Minnow net for dipping small fish from live well

1

Fish Collection

Multi-parameter meter with pH, DO, temp., and conductivity

1

Water Chemistry

Neutrally buoyant object (e.g., wiffle golf ball)



Discharge

Plastic bucket (or similar) with volume graduations (optional)

1

Discharge

Portable Weir and plastic sheeting to use with weir

1

Discharge

Seine (10' or 20' x 6' minnow/bag with mesh) (optional)



Fish Collection

Tape measure (in centimeters) (optional)

1

Physical Habitat

Top-set wading rod for use with current velocity meter

1

Physical Habitat

Watch with timer or stopwatch

1

General

Watchmakers' forceps

1

Benthics


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Sample Processing/Preservation

This is a summary of the supplies that will be used in sample processing/preservation. Items are
supplied in the Base Kit, Site Kit, Whole Fish Tissue Kit or by the Crew as indicated.

Item	Quantity Protocol



Aluminum foil squares (5"x 5")

1

Chlorophyll A

1—

Petri dishes (60 x 15, disposable)

20

Filters



Vacuum hand pump and clear plastic tubing

1

Chlorophyll A

£
<

47 mm polycarbonate 0.4 micron filters

1 box

Enterococci



Whatman 47 mm 0.7 micron GF/F glass fiber filters

1 box

Chlorophyll A



Whatman 47 mm 1.2 micron GF/C glass fiber filters

1 box

Periphyton



Aluminum foil (solvent-rinsed and baked)



Whole Fish Tissue

1—

Plastic Bags (large)

1

Shipping

LU

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Coolers



Storage/Shipping

o on

Polyethylene tubing (Heavy-duty food grade)



Whole Fish Tissue

^ X

Forceps (sterile, disposable)

2

Enterococci

t ^

Filtration unit (sterile 250 mL filter funnel, cap, and filter

1

Enterococci

	i

o

holder)





X

5

Phosphate buffered saline (sterile PBS)

1

Enterococci



Zip ties

10+

Whole Fish Tissue



Dry ice



Microcystin







Enterococci







Fish Tissue Plug







Whole Fish Tissue



Ethanol (95 %)



Benthics

o

LU

Fish Plug implements



Fish Plug

	1

Q_

Formalin (buffered, 10%)



Periphyton

z>





Fish Voucher



Knife or scissors

1

General

en

u

Scalpel for slitting open large fish before preservation

1

Fish Collection

Small spatula, spoon, or scoop to transfer sample

1

Benthics



Water (deionized)

1

General



Water (stream)

1

General



Wet ice



Water Chemistry







Chlorophyll A

Stock Solutions (provided by Sampling Crew)

SOLUTION

USE

PREPARATION

Bleach (1%)

Clean nets, other gear, and boat.

Add 40 mL bleach to 4 L distilled water.

Bleach (10%)

Clean periphyton sampling
equipment

Add 40 mL bleach to 400 mL distilled water.

10% Buffered
Formalint

Preservation of periphyton ID
sample and fixing Fish Vouchers

Formaldehyde (37-40%) 100 ml
Distilled water 900 ml
NaH2P04 4.0 g
Na2HP04 (anhydrous) 6.5 g
Mix to dissolve

95% Ethanol

Preservative for benthic invertebrate
samples and fish vouchers.

No preparation needed (use stock solution as is).

f 10% Buffered Formalin can also be purchased pre-mixedfrom various sources	<

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National Rivers and Streams Assessment 2018/19
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Field Operations Manual

Sample Storage

This is a summary of the supplies that will be used in sample storage, i.e. the container used in
shipping. These items are all provided in the site kit.

Item

Quantity

Protocol

Centrifuge tubes (sterile green screw-top, 50-mL)

4

Chlorophyll A
Periphyton

Cubitainer (4 L)

1

Water Chemistry

Scintillation glass vial (20 mL, acid washed)

1

Fish Tissue Plugs

HDPE bottle (1 L, white, wide-mouth)

1-2

Benthics

HDPE bottle (1 L, white, wide-mouth)

1

Fish Voucher

HDPE bottle (500 mL, white, round)
PETG bottle (500 mL, clear, square)

1

Microcystin/
Cylindrospermopsin

PETG bottle (125 mL, sterile, clear, square)

1

Periphyton PDNA

PET bottle (250 mL, sterile, clear, square)

1

Enterococci (temporary)

Microcentrifuge tubes containing sterile glass beads (sterile)

2

Enterococci

Packaging/Shipping

This is a summary of the supplies that will be used in the shipping of samples.

Item

Quantity

Protocol

Coolers

4

Shipping

Water Chemistry





Frozen Batched





Non-Chilled Batched





Whole Fish Tissue (at select sites only)





Cooler liners (for water chemistry & non-chilled batched shipments)

2

Shipping

Dry Ice Cooler Liner/Pad (for frozen batched shipments)

1

Shipping

Dangerous Goods label (Class 9, for dry ice shipments)

1-2

Shipping

Dry ice



Shipping

(~20 lbs per frozen batched shipment)





(~50 lbs per whole fish tissue shipment)





FedEx airbills (for specific sample groups)

3-4

Shipping

Packing tape

1

Shipping

Wet ice (~50 lbs per site; additional for shipping)



Shipping

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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Appendix B: Forms &

Labels

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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

The NRSA app will be preloaded on supplied iPads to record field data. Field crews will receive
labels packets with the site kits they request through the electronic Supply Request Form. Do
not mix labels from different sites.

Paper field forms will be supplied by NARS IM as backups to the App or for crews who choose
not to use the App. Please submit an electronic Supply Request Form well in advance of field
sampling - indicate if you would like wadeable or nonwadeable packets. For each site you will
receive a paper form packet with paper tracking forms. Prior to sampling, inspect each packet to
ensure all forms are included.

Item

Quantity

Protocol



Field forms packet (wadeable):



General



Verification (front & back)

1





Field Measurement

1





Sample Collection (front & back)

1





Fish Gear & Sampling Info (front & back)

1





Fish Collection

1





Whole Fish Tissue Collection (used at selected sites)

1





Physical Habitat: Channel/Riparian Cross-section - Wadeable Only

11+





Physical Habitat: Thalweg Profile & Woody Debris - Wadeable Only

11+





Physical Habitat: Slope & Bearing - Wadeable Only

1





Channel Constraint

1





Discharge - Wadeable Only

1





Torrent Evidence Assessment

1





Assessment

1





Field forms packet (nonwadeable):



General



Verification (front & back)

1





Field Measurement

1





Sample Collection (front & back)

1





Fish Gear & Sampling Info (front & back)

1





Fish Collection

1





Whole Fish Tissue Collection (used at selected sites)

1





Physical Habitat: Channel/Riparian Transect - Nonwadeble Only

10





Physical Habitat: Thalweg Profile - Nonwadeable Only

10





Channel Constraint

1





Torrent Evidence Assessment

1





Assessment

1





Packing Slips / Tracking forms & labels packet (all sites):



Shipping



T1 - Tracking: Site and Sample Status/Water Chemistry Lab Tracking

1





T2 - Tracking: Frozen Batch Samples - Overnight (dry ice)

1





T3 - Tracking: Non-Chilled Batch Samples - Ground (no ice)

1



C/")

	1

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T4 - Tracking: Whole Fish Tissue - Overnight (dry ice)

1



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T5 - Tracking: Packs

1



	1

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Labels (for samples)

1



Other forms (provided by NARS IM as needed):



General

cc

Fish Collection (additional)

10



O

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Seining Information (optional, if needed)





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Other forms (sent as update, if needed, by NARS IM after data packet





X

received): Fish Identification & Count

1

Fish

o

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Field Operations Manual

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Site ID:

NRSA 2018/19 VERIFICATION (Front)

Visit: O 1 O 2 Date:

Reviewed by (initial):.

Site Name:

State of Site Location:

Field Crew:

O This is a special State site

STREAM/RIVER VERIFICATION INFORMATION

Stream/River verified by (Mark all that apply): O GPS O Local Contact O Signs	O Roads O Topo. Map

O Other (Describe Here):

Coordinates

Latitude

Longitude

# of Satellites Elevation at transect A

GPS

Decimal Degrees
NAD 83

O —3 0>4

Location: o X-Site (wadeable) 0 Transect A (non-wadeablo

Oft O m

DID YOU SAMPLE THIS SITE?

OYES If Yes. check one below

O NO if No. check one below

SAMPLEABLE {Choose method used)

O Wadeable - Continuous water, greater than 50% wadeable

O Boatabie

O Partial - Sampled by wading (>50% of reach sampled). Explain below.
O Partial - Sampled by boat (>50% of reach sampled). Explain below.
O Wadeable Interrupted - Not continuous water along reach
O Boatabie interrupted - Not continuous water along reach
O Altered - Stream/River Channel Present but differs from Map

ADDITIONAL SITE CHARACTERISTICS
O Tidaiiy Influenced O Biackwater O Not Applicable

NON-SAIVIPLEABLE-PERMANENT

O Dry - Visited
O Dry - Not visited
O Wetland (No Definable Channel)

O Map Error (No evidence channel/waterbody ever present)
o Impounded (> 7 day residence time)

O Tidal (exceeds salinity threshold)

O Other (explain in comments)
NON-SAMPLEABLE-TEMPORARY

O Not boatabie - Need a different crew - Reschedule regardless of year
O Not wadeable - Need a different crew - Reschedule regardless of year
O Other (explain in comments)

NO ACCESS

O Access Permission Denied

O Permanently Inaccessible (Unable/Unsafe to Reach Site)
O Temporarily Inaccessible-Fire, etc. - Reschedule regardless of year
O other (explain in comments)

GENERAL COMMENTS

DIRECTIONS TO SITE

02/28/2018 NRSA18 Verification

7734319701

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National Rivers and Streams Assessment 2018/19
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Field Operations Manual

Channel Width
Used to Define
Reach(m)

DISTANCE (m) FROM X-SITE

Total Reach
Length Intended (m):

Comment:

Upstream Length:

Downstream Length:











r

Site ID:

NRSA 2018/19 VERIFICATION (Back)

Visit: 01 O 2 Date:

Reviewed by (initial}:^

/ /

STREAM/RIVER REACH DETERMINATION

PERSONNEL

Crew Leader:
Fish T axonomist:
Name:

Name:

Name:
Name:
Name:
Name:

02/28/2018 NRSA18 Verification

2492319707 ^

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Field Operations Manual

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Site ID:

NRSA 2018/19 FIELD MEASUREMENT

Date:

Reviewed by (initial):.

i	/

CALIBRATION INFORMATION

Instrument manufacturer and model:
Instrument ID number:

Thermometer
Reading (°C)

TEMPERATURE

i i	il i

Sensor
Reading (°C)

' >: nments

lEIevation

Barometric
Pressure fmm Ha)

Calibration Value

Displayed Value

. . 1 I , ! W

t i i i i

O mg/L
i i i i" i iO*

O mg/L

. . ,o%

DO

¦i vi indents

31. STD 1 Description

Cal. STD 1 Value	Cal. STD 2 Description	Cal. STD 2 Value

PH

J	I	!_l	I	L

J	I	LJ	I	L

:omments

¦aI. STD 1 Description

Cal. STD 1 Value	Cai. STD 2 Description	Cal. STD 2 Value

CONDUCTIVITY

;omments

FIELD MEASUREMENT OX-Site (wadenble) O Transect A (non-wodenhie) O Other Transect:

_j	i	i_i	i	

DO(mg/L) XXX

Temp. (°C) XX.X

i	i	i	*

pHXXXX

Cond. (pS/cm) XXX

Corrected to 25°C ? O f On

02/28/2018 NRSA18 Field Measurement

2683415065

B-6


-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Site ID:

NRSA 2018/19 SAMPLE COLLECTION (Front)

Date:	/



CHEMISTRY (CHEM) STATION COLLECTED:

(Target Volume" 4L) q X-She (w > i »

\ uliimM Filtered (ml)[ Frozen Comments

No Sample Collected Q

PERIPHYTON METAGEN9MIC (PPNA) (PETG bottle)

No Sample Collected O

Sample ID

F ro z
-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

CO

<
	I

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m

5

QC

o

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Q

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<

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^ NRSA 2018/19 SAMPLE COLLECTION (Back) Q

Site ID: Date: / /

BENTHIC MACROINVERTEBRATES (BERW) - WADEABLE No Sample Collected Q

Sample ID

Number of jars

Preserves
(ETOH)

No. of
Transects

Comments

I I I i i I I



o





REACH-WIDE BENTHOS - WADEABLE

TRANSECT

A

B

C

D

E

F

G

H

1

J

K

SUBSTRATE

CHAN.

Sub.

Chan.

Sub.

Chan.

Sub.

Chan,

Sub.

Chan.

sub.

Chan.

Sub.

Chan.

Sub.

Chan.

Sub.

Chan.

Sub.

Chan.

Sub.

Chan.

Sub.

Chan.

Fine/Sand

Pool

Qf

Of

Of

O P

Of

Of

Of

Of

Of

Of

O F

Op

Of

Op

Of

Op

Of

Op

Of

Op

Of

Op

Gravel

Glide

o ®

OOL

Os

Ogl

Og

O GL

o °

o gl

Og

O GL

O G

O GL

O G

O GL

O G

Ogl

Og

O gl

O G

O gl

O G

O GL

Coarse

Riffle

Oc

o»

Oc

O™

Oc

o»

Oc

O R|

Oc

OR'

Oc

OR'

Oc

o»

Oc

O Ri

Oc

Ori

Oc

Ori

Oc

Ori

Other:

Rapid

O 0T

Ora

O °T

O RA

O °1

O RA

O OT

O RA

Oot

O RA

O 0T

O RA

O OT

O RA

O OT

O RA

O OT

O RA

O OT

QRA

O OT

O RA

If other, flag arid
explain in comments

Flag

Flag

Flag

Flag

Flag

Flag

Flag

Flag

Flag

Flag

Flag























BENTHIC MACROINVERTEBRATES (BETB) - BOATABLE No Sample Collected Q

Sample ID

Number of jars

Preserved
(ETO'H)

No. of
Transects

Comments

i i i i i i i



O





TRANSECT BENTHOS - BOATABLE

Habitat; C = Coarse Substrate / LWD L = Leaf Pack F = Organic Fine Muds / Sand M = Macrophyte beds OT = Other (Flag and explain in comment section below)
Substrate: F = Fine / Sand G = Gravel C = Coarse substrate OT = Other (Flag and explain in comment section beiow)

Channel? P = Pool GL = Glide Rl = Riffle RA = Rapid OT = Other (Flag and explain in comment section below)

TRANSECT
Location (L/R):

A

O L OR

B

Ol OR

c

Oi- OR

D

Ol Or

E

OL OR

F

ot- Or

G

Ol Or

H

Ol Or

1

Oi- Or

J

Ol Or

K

Ol or

Dominant
Habitat:

{ONE PER TRANSECT*

O C Ol

Of 0»

O 0T

Oc O'
Of Om

OOT

Oc Ol
Of 0»

O OT

Oc Ol
Of Om

O ot

Oc Ol
Of Om

O OT

Oc Ol
Of Om

O OT

Oc Ol
Of Om

O OT

Oc Ol
Of Om

O OT

Oc Ol
Of Om

O OT

O C O L
Of Om

O OT

Oc Ol
Of Om

O OT

Secondary
Habitat:

(ON E PER TRANSECT)

Oc Ol
Of Oh

Qot

O G Ol
Of Ok

Oot

Oc Ol
Of Om

O OT

Oc Ol
Of Om

Oot

Oc Ol
Of Om

Oot

Oc Ol

Of O m

0°t

Oc Ol
Of O m

Oot

O C O L

Of Om

Oot

Oc Ol
Of Om

O OT

Oc Ol
Of Om

Oot

OC Ol
Of O m

O OT

Substrate:

(ONE PER TRANSECT)

Of Oc

O o O OT

Of Oc

O o O OT

Of Oc
O G O 01

Of Oc

Og Oot

Of Oc

O G O OT

Of Oc

Og Oot

Of Oc

Og Oot

Of Oc

O G O OT

Of Oc

Oe Oot

Of Oc

O G o OT

Of Oc

Og Oot

Channei:


-------
Site ID:

NRSA 2018/19 FISH GEAR AND SAMPLING INFORMATION (Front)

Date:

Renewed by (initial): _
/

FISH SAMPLING PROTOCOL (select one):

FISH SAMPLING - NOT CONDUCTED OR SUSPENDED (select one):

O Large Nonwadeable- (> 13m wide)
O Small Nonwadeable - (< 12m wide)
O Larg e Wadeab le - (> 13m wide)
O Small Wadeable - (<12m wide)

O Fast flowing high gradient site

O Fished - None Collected	O Not Fished - No Permit

O Not Fished/Fishing suspended - Can't sample > 50% of	° Not Fished" site Conditions Prohibit Sampling (Describe in comments)

required reach:	O Fishing Suspended - Permit Restriction (Listed species encountered)

-	Nonwadeable and Lg. Wadeable (at least 10 CW)	O Not Fished - Equipment Failure

-	Wadeable (at least 20 CW)	O Not Fished - No fish observed (applies to small wadeable streams only)

Did conditions allow for OV ON
sufficient sampling? Q Not Sure

Fish Response to q Immobilized O Inhibited Swimming O Escape
Electrofishing

Final Length of Fishing Reach (m):

Sampling Protocol Comments:

FISH GEAR INFORMATION

Water Visibility: OGood OPoor Water Temp (°C):

Specific Conductivity (uS/cm):

Corrected to 25"C ? O y On

Primary Electrofishing Gear Model:

O BOAT (Motor)

O RAFT (No motor)

O BACKPACK
O BANKOR TOWED UNIT Diameter

# of Netters (1):

Anodes

Number:

Wave Form: O AC O DC

O Pulsed DC

Volts: (50-1000)

Watts: likely 400 (bp),
2500 or 5000 (boatfraft) L

O in. % of Power

. O Cm {Smith Root GPP only)

Pulse Rate:
pps or Hz

Amps: (may not be
	provided for bp) .

OR O High OLow Pulse Width (ms):
Total Shock (button) Time (s):

Total Fishing Time (min):

Length Sampled (m):

Secondary Electrofishing Gear Model:

O BOAT (Motor)

O RAFT (No motor)

O BACKPACK
O BANKOR TOWED UNIT Diameter

» of Netters (1):
Anodes

Number:

Wave Form: O AC O DC

O Pulsed DC

Volts: (50-1000)

Watts: likely 400 (bp),
2500 or 5000 (boat/raft) L

OR O High OLow Pulse Width (ms):
Total Shock (button) Time (s):

O i»- % of Power

O cm (Smith Root GPP only)

Pulse Rate:
pps or Hz
Amps: (may not be
provided for bp) ,		

Total Fishing Time (min):

Length Sampled (m):

Primary Seine Net: O BAG SEINE O MINNOW SEINE No. of crew members:
Height (m):	Length (m):	Mesh (mm):

Avg. Haul
Len nth (m):.

No. of
Hauls:

Total Seining
Time (min):

Length Sampled (m):

Secondary Seine Net: O BAG SEINE O MINNOW SEINE No. of crew members:
Height (m):	Length (m):	Mesh (mm):

Avg. Haul
Length (m):.

No. of
Hauls:

Total Seining
Time (min):

Length Sampled (m):

GEAR INFORMATION COMMENTS

03/28/2018 NRSA18 Fish Gear and Sampling Information

0788063X31

APPENDIX B: FORMS & LABELS


-------
^ APPENDIX B: FORMS & LABELS
o

¦ NRSA 2018/19 FISH GEAR AND SAMPLING INFORMATION (Back) "*"**"b"""' ¦

Site ID: Date: 1 1

QA VOUCHER SAMPLE INFORMATION (VERT)

NO VOUCHERS PRESERVED O

Sample ID

# of Jars

Preserved

Comments





o



PHOTO VOUCHER FILE INFORMATION

Page

Line

Photo File Name (SitelD V(Visit#) Tag#)
(e.g.: NRS18_OR_11158_V1_Tag01)

Sequence
(e.g. a-c)

Comments

Page

Line

Photo File Name (SitelD V(Visit#) Tag#)
(e.g.: NRS18_OR_11158_V1_Tag01)

Sequence
(e.g. a-c)

Comments





















































































































































































FISH TISSUE PLUG SAMPLES (FPLG)

NO SAMPLE COLLECTED O

Collection Method: O ELECTROFISHING O HOOK & LINE O SEINING O OTHER:



Sample ID

Common Name

Length(mm)

Weight(g)

Comments



















Q3£8£018 NRSA18 Fish Gear and Sampling Information

1604063189 £

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-------
¦

NRSA 2018/19 FISH COLLE

Site ID: Date: / /

CTK

DN

3age of

Reviewed bWinitian:

I did not evaluate the native/
introduced status for fish collection. O Y

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duced?

Tally and Counts

Anom
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Mortality
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VOUCHER

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Tag#

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Retained

Flag

<150 mm

150-300 mm

300-460 mm

>460 mm

Unk/Rng

OA

Photo

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o

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0

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o





















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o







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4

5

















o

0

o







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o

o

¦

















o

o

o







Flag

Com ments

Flag

Comments

























































Flag codes: K = No measurement made, F1.F2, etc. = flags assigned by each field crew. Explain all flags in comments.

A 2194249191 A
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ti APPENDIX B: FORMS & LABELS


-------
National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

¦ NRSA 2018/19 WHOLE FISH TISSUE COLLECTION

O WAOEABLF O BOATABLE

Reviewed by (initial}:	Hj

Site ID: Date: /



/

PAGE: OF

WHOLE FISH TISSUE FILLET SAMPLE (FTIS) SAMPLE ID:



NO SAMPLE COLLECTED Q

O FISH ARE ALL THE SAME SPE CIES Q FISH ALL WITHIN 75% OF- LARGEST SPECIMEN

I ! Total
| Common Name i Lencjth (mm)

Fiozen



Comments

01

o





.02

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.03

o



.04

o



.05

o



.06*

o



.07*

o



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.or

O



,10* i

o



"Additional specimens for smaller fish species to ensure sufficient tissue is asailable for chemical analysis of fillet tissue.



II 03/2S/2018 NRSA18 Fish Tissue Collection





9371581329 £


-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Reviewed by (initial): _

^	NRSA 2018/19 PHAB: CHANNEL/RIPARIAN TRANSECT (Front) - NONWADEABLE ONLY

Site ID:	Date:	/	/

TRANSECT: OA OB OC OD OE OF OG OH Ol OJ OK

Chosen bank side:

O Left O Right

BANK Decimal Degrees Latitude

MIDSTREAM Decimal Degrees Latitude

Longitude

Longitude

•LITTORAL" and SHORELINE SUBSTRATE INFORMATION

DEPTH Oft Qm

SHORE

DOM SEC

o

BOTTOM

DOM SEC

o

BOTTOM SUBSTRATE FROM (X ONE):
CLASS o Judgement-or- O OBS. @ S Littoral Depths

Flag

RS = Bedrock (Smooth) - (Larger than a car)

RR =

Bedrock ( Rough) - (Larger than a car)

XB = Large Boulder{1000 to4000 mm) - (Meterstickto carl

SB - Small Boulder (250 to 1000 mmj - (Basketball to Meterstlck)

CB = Cobble (64 to 250 mm) - (Tennis ball to Basketball)

GC = Coarse Gravel (16 to 64 mm) - (Marble to Tennis ball)

GF = Fine Gravel (2 to 16 mm) - (Ladybug to marble)

SONAR

xx.x

POLE
X-X

SA = Sand (0.06 to 2 mm) - (Gritty - up to Ladybug size)

BANK CHARACTERISTICS

FN * silt / Clay / Muck - (Not Gritty)

HP = Hardpan - (Firm, Consolidated Fine Substrate)

Wetted Width XX.XX (m)

WD = Wood - (Any Size)

Bar Width X.XX (m)

o

o

OT = Other (Write comment below)

Bankfull Width X.XX (m)

DEBRIS

>t) Tally each piece

FILL IN IF UNMARKED BOXES ARE ZERO

o

FLAG:

Bankfull Height X.XX (m)

DIAMETER
LARGE END

Wood All/Part in Wetted Channel

Dry by Ali/Part in Bankfull Channel

Length 5-15m

15-30m

>30m

Length 5-15m

15-30m

>30m

0.3-0.6 m

















0.8-0.8 m

















0.8-1.0 m

















>1.0m

















Incised Height XX.X (m)

INTENDED transect spacing xxx (m):

ACTUAL transect spacing xxx (m): |

BANK
ANGLE

CIRCLE ONE

Ov
Os
O g
OF

Flag

Comments

Flag Codes: U - suspect or non-standard measurement; F1, F2, etc. = flags assigned by each field crew. Explain all flags in comments section.
02/2S/201S NR5A18 Phab Channel Riparian - Nonwadeabfe (Front)	2635312080


-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

CD

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• NRSA 2018/19 PHAB: CHANNEL/RIPARIAN TRANSECT (Back) - NONWADEABLE ONLY •

Site ID: Date: / /

TRANSECT: OA OB OC OD OE OF OG OH Ol OJ OK O Left O Right

VISUAL RIPARIAN ESTIMATES

0 = Absent (0%) 1 = Sparse (<10%) 2 = Moderate (10-40%) 3 = Heavy (40-75%)
4 = Very Heavy (>75%)

D = Deciduous C = Coniferous E = Broadleaf Evergreen M = Mixed N = None

FISH COVER (10m x 20m Plot)

0 = Absent (0%) 1 = Sparse (<10%) 2 = Moderate (10-40%) 3 = Heavy (40-75%)
4 = Very Heavy (>75%)

ln-channel Cover Flag

RIPARIAN VEGETATION COVER

Canopy (>5 m high) Left Bank Flag Right Bank Flag

Filamentous Algae

OOOOO



Macrophytes

ooooo



Woody Vegetation Type

ooooo



ooooo



Woody Debris >0.3 m (BIG)

ooooo



BIG Trees (Trunk
>0.3 m DBH)

ooooo



ooooo



Brush/Woody Debris <0.3 m (SMALL)

ooooo



SMALL Trees (Trunk

ooooo



ooooo



Understory (0.5 to 5 m

igh)







Live Trees in Stream

ooooo



Woody Vegetation Type

ooooo



ooooo



Overhanging Veg. <1 m of Surface

ooooo



Woody Shrubs & Saplings

ooooo



ooooo



Undercut Banks

ooooo



Non-Woody Herbs,
Grasses, & Forbs

ooooo



ooooo



Boulders/Ledges

ooooo



Ground Cover (<0.5 m high)

Artificial Structures

ooooo



Woody Shrubs
& Saplings

ooooo



ooooo



CHANNEL CONSTRAINT

Non-Woody Herbs,
Grasses and Forbs

ooooo



ooooo



Barren, Bare Dirt
or Duff

ooooo



ooooo



Distance from shore to riparian vegetation (m) XXX



HUMAN INFLUENCE 0 = Not Present P = >10 m C = Within 10 m plot B = On Bank

Mark only one:

Flag

O Channel is Constrained





Wall/Dike/Revetment
/Riprap/Dam

oooo



oooo





Buildings

oooo



oooo



O Channel is in Narrow Valley but NOT very constrained
O Channel is Unconstrained in Broad Valley

Pavement/Cleared Lot

oooo



oooo



Road/Railroad

oooo



oooo



Mark only one:





Pipes (Inlet/Outlet)

oooo



oooo



O Yes, I could readily see over the bank Flag





Landfill/Trash

oooo



oooo



O No, I could not readily see over the bank





Park/Lawn

oooo



oooo



CANOPY DENSITY @ BANK

DENSIOMETER (0-17Max)

Row Crops

oooo



oooo



Pasture/Range/Hay Field

oooo



oooo





Flag



Flag



Up
Down





Left
Right







Logging Operations

oooo



oooo



Mining Activity

oooo



oooo











Flag

Comments





























Flag Codes: U = suspect or non-standard measurement; F1, F2, etc. = flags assigned by each field crew. Explain all flags in comments section.
02/28/2018 NRSA18 Phab Channel Riparian - Nonwadeable (Back) 9930297177


-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Site ID:

NRSA 2018/19 PHAB: THALWEG PROFILE - NONWADEABLE ONLY

Date:	/	f

TRANSECT: OA-B 0 B-C 0 C-D 0 D-E OE-F 0 F-G C G-H 0 H-l 0 l-J 0 J-K

SUBSTRATE CODES

CHANNEL HABITAT CODES

OTHER

BII *= BEDROCKHARDPAH (SMOOTH OR ROUGH) (LARGER TO AH A CAR)

BL = BOULDER 
-------
^ APPENDIX B: FORMS & LABELS

cn

Site ID:

NRSA 2018/19 PHAB: CHANNEL/RIPARIAN CROSS-SECTION - WADEABLE ONLY Reviewed by 75%)

Cover in Channel

Flag

Filamentous Algae

Macrophytes

Woody Debris
>0.3 m (BIG)

Brush/Woody Debris
<0.3 m (SMALL)

Live Trees or Roots

Overhanging Veg.
=<1 m ol Surface

Undercut Banks

Artificial Structwes

ooooo

ooooo

OGOOO

ooooo

ooooo

ooooo

ooooo

VISUAL RIPARIAN ESTIMATES

0 = Absent (0%) 1 = Sparse (<10%) 2 = Moderate (10-40%) 3 = Heavy (40-75%)
4 = VeryHeavy (>75%)

D = Deciduous C = Coniferous E = Broadleaf Evergreen M = Mixed N = None

RIPARIAN VEGETATION COVER
Canopy (>5 m high, Left Bank

Woody Veg^tati or fypei Q Q Q (T)

BIG Trt ~0
-0.31. DBn,

'*00000

SMA L Tr ^s (li .nk
.0.3 ) DBH)

indb "to. / 'ft r to 5 m high)

vk ody v 'jet at ion Type

Woody Shrubs &
Saplings

OOOOO
O O O J D

CANOPY COVER MEASUREMENTS

DENSIOMETER (0-17Ma:
Flag

Flag

CenUp



' U?nR





CenL

tf



Left





CenDwn

\

%

Right





OOOOO

Flag: Right Bank

Flag:

OOOOO

ooooo

ooooo

ooooo

ooooo

Non-Woody Herbs. ^ ^ >-v
Grasses. & Forbs w U U W U

Ground Cover (<0.5 m high)

OOOOO

ooooo

ooooo

Woody Shrubs
& Saplings

Non-Woody Herbs,
Grasses and Forbs

Barren, Bare Dirt
or Duff

ooooo

ooooo

ooooo

ooooo

ooooo

ooooo

HUMAN INFLUENCE 0 = Not Present P = >10 m C = Within 10 m plot B = On Bank

Wal I/Dike/ Revetment
	/Riprap/Dam

Buildings

Pavement/Cleared Lot

Road/Railroad

Pipes (Inlet/Outlet)

Landfill/Trash

Row Crops

Pasture/Range/Hay Field

oooo

o o o ©

oooo

oooo

oooo

oooo

oooo

oooo

oooo

oooo

oooo

oooo

oooo

oooo

oooo

oooo

oooo

oooo

Logging Operations

oooo

oooo

Mining Activity

oooo

oooo

Flag codes: K = Sample not collected; U = Suspect sample; F1, F2, etc. = flag assigned by field crew. Explain all flags in comment sections.

09/11/2017 NRSA18 Phab Channel Riparian - Wadeable

4803036914

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-------
Site ID:

NRSA 2018/19 PHAB: THALWEG PROFILE

Date:	/	/

& WOODY DEBRIS - WADEABLE ONLY

O A-B O B-C O C-D

Reviewed by (initial):.

TRANSECT:

O F-G O G-H O H-l

OD-E
Ol-J

OE-F
OJ-K

THALWEG PROFILE

For Transect A-B ONLY:lncrement (m) X.X:

Total Reach Length (m):

STA-
TION

THALWEG
DEPTH (cm)
(XXX)



BAR WIDTH 1

SOFT
/SMALL
SEDI-
MENT

CHANNEL
UNIT CODE

SIDE
CHANNEL

BACK
WATER

THALWEG PROFILE COMMENTS

WETTED WIDTH

(m) (XXXJ0

Present

XX.X

0





o



o



O

O



1





o



o



O

O



2



o

o



O

O



3



o

o



O

O

> (?>

4



o

o



O

O

xNr

*5





o



o



O

O

V

6





o



o



O

o

. A,

*7





o



o



O

o

0.8 m

1 = Measure Bar Width at Station 0 and Mid-Station {5 or 7).

Rag Codes: K = no measurement made; U = suspect measurement; F1, F2, etc. = flags assigned by each field crew. Explain all flags in comments.
10/04/2017 NRSA18 Phab Thalweg Profile - Wadeable

6364573600

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APPENDIX B: FORMS & LABELS


-------
^ APPENDIX B: FORMS & LABELS
oo

m NRSA 2018/19 SEINING INFORMATION •>***«

Site ID: Date: / / Page of

Line
#

Subreach
(e.g., A-B)

Bank

Habitat Sampled (choose one) If other, describe:

Seine
Width (m)

Seine
Depth (m)

Appro x
Haul
Distance (r

Appro x
Haul
l) Time (sec

Fish

Collected

Additional
? Persor

Flag

1



O L o R O CH

O PL OGL O RF ~
OBW OED O SN O0,her'









o

o



2



O L o R O CH

O PL OGL O RF
OBW OED O SN °0,her'









o

o



3



O L o R O CH

O PL OGL O RF _ „ ,
OBW OED O SN O0,her:









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o



4



OL OR OCH

O PL OGL O RF ,
OBW OED O SN O0,her:









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o



5



o L o R O CH

O PL OGL O RF
OBW OED O SN s-,u,l,er-









o

o



6



Ol Or Och

O PL OGL O RF
OBW OED O SN O0,her'









o

o



7



O L o R O CH

O PL OGL O RF
OBW OED O SN O0,her'









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o



8



O L O R O CH

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OBW OED O SN °°,her-









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o



9



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OBW OED O SN O0,her'









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o



10



OL OR OCH

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OBW OED O SN °°,her-









o

o



11



OL OR OCH

O PL OGL O RF
OBW OED O SN °0,her-









o

o



12



OL OR OCH

O PL OGL O RF
OBW OED O SN °0,her-









o

o



13



o L o R O CH

O PL OGL O RF _ „ ,
OBW OED O SN °0,her:









o

o



14



o L o R O CH

O PL OGL O RF
OBW OED O SN O0,her'









o

o



15



O L o R O CH

O PL OGL O RF
OBW OED O SN °0,her:









o

o



BANK L = Left Bank (facing downstream R = Right Bank (facing downstream) CH = In Channel

HABITAT SAMPLED: PL= Pool, GL= Glide/Run, RF = Riffle, ED = Edge, SN = Snag, BW= Backwater or side channel, OT = Other (describe)

FLAG CODES: K= No measurement made, U = Suspect measurement., F1 ,F2, etc. = flags assigned by each field crew. Explain all flags in comments.
Flag Comments

















£ 03/28/2018 NRSA18 Seining Information 43030369 = 5 %

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NRSA 2018/19 PHAB: SLOPE AND BEARING - WADEABLE ONLY



Reviewed by (initial):



¦



Site ID:







Date:



/ /





MAIN (always used)

FIRST SUPPLEMENTAL

SECOND SUPPLEMENTAL



TRANSECT & METHOD

Mark method for every Transect

Slope(%) or Elev.

Diff. (cm)

Mark Units for every Transect

BEARING

0-359

PROPOR-
TION %

Slope(%) or
Elev. Dlff. (cm)

BEARING

0-359

PROPOR-
TION %

Slope(%) or
Elev. Diff. (cm)

BEARING
0-359

PROPOR-
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Flag

Comments

FLOW )

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Supple-
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ain
\

















Flag codes: U = Suspect sample; F1, F2, M (M = Method - used for method comment only) = flag assigned by field crew. Explain all flags in comment sections.
A CL=Clinometer; HL=Hand Level; LA=Laser rangefinder with electronic clinometer; TR=Transit, surveyors level or total station; WT=Water Tubing. 0181146753

09/11/2017 NRSA18 Slope and Bearing

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S APPENDIX B: FORMS & LABELS


-------
National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

H	NRSA 2018/19 CHANNEL CONSTRAINT	Revievsed by (initial): _

Site ID:	Date:	/	/

CHANNEL PATTERN (Fill in one):

O One Channel

O Anastomosing (complex) channel - (Relatively long major and minor channels branching and rejoining.)

O Braided channel - (Multiple short channels branching and rejoining - mainly one channel broken up by
numerous mid-channel bars.)

CHANNEL CONSTRAINT(Fill in one):

O Channel very constrained in V-shaped valley (i.e. it is very unlikely to spread out over valley or erode a

new channel during flood)

O Channel is in Broad Valley but channel movement by erosion during floods is constrained by Incision

(Flood flows do not commonly spread over valley floor or into multiple channels.)

O Channel is in Marrow Valley but is not very constrained, but limited in movement by relatively narrow

valley floor(< ~1Q xbankfull width)

O Channel is Unconstrained in Broad Valley (i.e. during flood it can fill off-channel areas and side channels,
spread out over flood plain, or easily cut new channels by erosion)

O Bedrock (i.e. channel is a bedrock-dominated gorge)

O Hillslope (i.e. channel constrained in narrowV-shaped valley)

OTerrace (i.e. channel is constrained by its own incision into river/stream gravel/soil deposits)
O Human Bank Alterations (i.e. constrained by rip-rap. landfill, dike, road, etc.)
O No constraining features

Percent of channel length with margin
in contact with constraining feature:

%

(0-100%)

Bankfull width:

(m)

imj

Valley width (Visual Estimated Average):

Note: Be sure to include distances between both sides of valley bolder for valley width.
Ifyou cannot seethe valley borders, record the distance
you can see and fill this bubble:

COMMENTS

B-20


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Site ID:

NRSA 2018/19 DISCHARGE -WADEABLE ONLY

Date:	/	/

O Velocity Area

Distance Units
O ft O cm
Dist. from Bank

Depth Units
O ft O cm
Depth

Velocity Units
OftsXX.X QmsX.XX

Velocity

Flag

OQ Value

If discharge is determined directly
in field, record value here: Q =

1

0 |





2

I





3

I





4

I





5

6

7
3
9

t
i

			

" |

	







10

11





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12

13





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14





t

t

15





16

i

I

I

17



i

18

19

20









Flag

Comments

O Timed Filling

Repeat Volume (Li Time(s)

1	I ¦

2

_i	I	I	1^1	I J	

Flag

r a 	i t

O Neutral Buoyant Object

Measure the stream depth using a wading rod or meter stick at
points approximately equal to the folio»ving proportions of the total
width: 0.1. 0.3, 0.5. 0.7. and 0.9.

Float Dist.

Oft O m

Final Time
(*)

Flai|

Float 1

Float 2

Float 3

Cross Sections on Float Reach

Width
Oft O m

Depth 1

O ft O cm

Depth ?

Depth 1

Depth 4

Depth 5

Upper Section

Middle Section

Lower Section

O cfs
_0 m'.'s

Flag

Flag Codes: 2 = Last station measured (if not Station 20): F1, Fj, etc. = Miscellaneous flags assigned by each field crew.

Explain all flags in comments section.	5312491487

03/28/2018 NRSA1S Discharge


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Reviewed bv (initial*: —

¦ NRSA 2018/19 TORRENT EVIDENCE ASSESSMENT ¦

Site ID: Date; / /

Flense fill in any of the following that are evident.
EVIDENCE OF TORRENT SCOURING:

O

01 - Stream channel has a recently devegetated corridor two or more times the width of the low flow channel. This
corridor lacks riparian vegetation with possible exception of fireweed. even-aged alder or Cottonwood seedlings, grasses,
or olhei herbaceous plants.

o

02 - Stream substrate cobbles or large gravel particles are NOT IMBRICATED. (Imbricated means that they lie with flat
sides horizontal and that they are stacked like roof shingles - imagine the upstream direction as the top of the "roof.") In
a torrent scour or deposition channel, the stories are laying in unorganized patterns, lying "every which way," In addition
many of the substrate particles are angular (not "water-worn.")

o

03 - Channel has little evidence of pool-riffle structure. (For example, could you ride a mountain bike down the channel?)

o

04 - The stream channel is scoured down to bedrock for substantial portion of reach.

o

05 - There are gravel or cobble terms (little levees) above bankfull level.

o

06 - Downstream of the scoured reach (possibly several miles), there arc rrc? s>ive c " osi ; of sediment, logs, and other
debris.

o

07 - Riparian trees have fresh bark scars at many points along the stream at se "riin^ly unbelievable heights above the
channel bed.

o

08 - Riparian trees have fallen Into the channel as a result of see irir,. near Seir roots,

EVIDENCE OF TORRENT DEPOSITS:

09 - There are massive deposits of sediment, logs, arid otlv. u. "iris in the reach. They may contain wood and boulders
that in your judgement, could not have boon moved by the s' eai ! at even extreme flood stage.

10 - if the stream has begun to erode newly laid depo .its "'s evident that these deposits are "MATRIX SUPPORTED."
j-. This means that the large particles, like boulders ; id *.obb' ,-s. are often not touching each other, but have silt, sand, and
other fine particles between them (their weight oi >cd by these fine particles -- in contrast to a normal stream
deposit, where fines, it present, normally "fill-in 'he i1 i.erstices between coarser particles.)

i NO EVIDENCE.

Q II ¦ No (>vidi*iicp «f tcnrnl scourint ji sr.i >nl deposits.

COMMENTS

CL
CL
<

10/04/201? NRSA1S Torrent Evidence

B-22

5543621376


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

¦	Reviewed by (initial):	

NRSA 2018/19 ASSESSMENT (Front)		 ¦

Site ID:	Date:	/	/

Elevation at transecl K:	Oil Om

WATERSHED ACTIVITIES AND DISTURBANCES OBSERVED

(Intensity: Blank=Not observed. L=Low: M=Hoderate: H=Heavy)

Rpsidi-nlial

Recreational

Agricultural

liulusliia!

Stream Management

0

©

© Residences

©

©

© Hiking Trails

©

©

© Cropland

0

©

© Industrial Plants

©

©

© Liming

0

©

0 Maintained Lawns

0

©

© Parks, Campgrounds

0

©

© Pasture

0

©

0 Mines/Quarries

0

©

© Chemical Treatment

0

©

0 Construction

O

©

© Primitive Parks, Camping

0

©

© Livestock Use

0

©

© Oil/Gas Wells

©

©

© Angling Pressure

O

©

© Pipes, Drains

o

©

© Trash/Litter

0

©

© Orchards

0

©

0 Power Plants

©

©

© Dredgong

0

©

0 Dumping

©

©

© Surface Films, Scums,

0

©

© Poultry

0

©

© Logging

©

©

© Channelization

0

©

0 Roads





or Slicks

0

©

© Feedlot

0

©

© Evidence of Fir-

O

O

© Water Level Fluctuations

0

©

0 Bridges/Causeway







0

©

© Water Withdrawa

0

©

© Odors



©

© Fish Stocking

0

©

0 Sewage Treatment













0

©

© Comnu -i?J

©



© Dams

SITE CHARACTERISTICS (200m radius)

WATERBODY CHARACTER

PRISTINE: O 5 04 O 3 02 O 1 Highly Disturbed
APPEALING: O 5 04 O 3 O 2 01 Unappealing
BEAVER

Beaver Signs: O Absent O Rare O Common
Beaver Flow Modifications: O None O Minor O Major

DOMINANT LAND USE	

Dominant Land Use Around 'X' O"--: O
If Forest. Dominant Age Class O
WEATHER

1 ¦¦ ¦ O	O ~ : .

O

CONDITIONS AND Lf ~AL L, ~>N . ,-OTS

OBSERVATIONS (e.g. accessibility, boating, fishing, swimming, health concerns):

09/13/2017 NRSA IS Assessment

4803036903 |


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Site ID:

NRSA 2018/19 ASSESSMENT (Back)
Date:	/

Reviewed by (initial):.

GENERAL ASSESSMENT AND COMMENTS





-

-

—







INVASIVE OR NUISANCE SPECIES L LOt. \L INTEREST

Record species of plants and animal® that we. o observed but are riot on the invasive plant form. Examples would bo Zebra Mussel or
New Zealand Mud Snail, or invasi' pit. ts or animals of concern to a particular state. Indicate your level of confidence in your
identification, and provide it --a of iok prevalent it is in the sampling reach or adjacent riparian area.

Species (Common Na. <>)

Confidence

Prevalence

Comments

O LOW
O HIGH

o DOMINANT o SPARSE
OCOMMON

O LOW
Q HIGH

O DOMINANT o SPARSE
OCOMMON

O LOW
O HIGH

O DOMINANT O SPARSE

OCOMMON

O LOW

O HIGH

O DOMINANT O SPARSE
OCOMMON

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O LOW
O HIGH

O DOMINANT O SPARSE
OCOMMON

O LOW
O HIGH

O DOMINANT O SPARSE
OCOMMON

O LOW
O HIGH

O DOMINANT O sparse
OCOMMON

O LOW

O HIGH

O DOMINANT O SPARSE
OCOMMON

09/13/201? NRSA1S Assessment

4860036906 £

CL
CL
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B-24


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

WATER CHEMISTRY (CHEM)

Site ID: NRS18	

Date:	/	/201	 Visit#: 01 02

t 1	990000

WCHL, PCHL, PBIO - OUTER BAG

Site ID: NRS18	

Date:	/	/201	 Visit#: 01 02

990001,990003,990004

T1

CHLOROPHYLL-a (WCHL)

Site ID: NRS18	

Date:	/201 Visit#: 01 02

Volume Filtered: 	mL

ti	990001

PERIPHYTON ASSEMBLAGE ID (PERI)

Site ID: NRS18	

Date;	/	/201	 Visit#: 01 02

Composite Volume: 	mL

T3	990002

PERIPHYTON CHLOROPHYLL (PCHL)

Site ID: NRS18

Date:

__/	/201	 Visit#: 01 02

Volume Filtered: 	mL

990003

PERIPHYTON METAGENOMIC (PDNA)

Site ID: NRS18	

Date:

/201

Visit#: 01 02

990005

ALGAL TOXIN (MICZ)

(HDPE Bottle (round Nalgene bottle)
Site ID: NRS18 	

Date:

/

/201 Visit #: 01 02

990007

BENTHIC MACROINVERTEBRATES (BETB)

Site ID: NRS18	

Date:	/

T3

_/201	 Visit#: 01 02

990009

PERIPHYTON BIOMASS (PBIO)

Site ID: NRS18 	

Date:

/

	_/201	 Visit#: 01 02

Volume Filtered: 	mL

990004

ALGAL TOXIN (MICX)

(PETG Bottle (clear, square bottle)
Site ID: NRS18 	

Date:

/

/201 Visit#: 01 02

T2	990006

BENTHIC MACROINVERTEBRATES (BERW)

Site ID: NRS18	

Date: / /201 Visit#: 01 02
is	990008

FISH TISSUE PLUG (FPLG)

Site ID: NRS18	

Date: /

T2

	/201	 Visit #: 01 02

990011

QA VOUCHER (VERT)

Site ID: NRS18	

Date: / /2Q1 Visit#: Oi 02

990012

UNK/RNG VOUCHER (VERT)

Site ID: NRS18	

Date: / /2Q1 Visit#: 01 02


-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

> it ¦ :

Vol.	ml

ENTEROCOCCI (ENTE) - BAG	..uTj"

Site ID: NRS18

Date: / /201 Visit #: 01 02	- -

Vol. • It _ml

Vol. Fill: 1	ml 2	 ml	•.•ua.i

990010

		 ik

Vol	_ ml

Q-
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FISH TISSUE FILLET (FTIS)
Site ID: NRS18 	

Date:

/

_/201_

Length:_

Visit#: 01 02

mm

T4

999013,1

FISH TISSUE FILLET (FTIS)
Site ID: NRS18 	

Date:

/

_/2Q1

Length: _

T4

999013.2

Visit #:Q1 02
mm

FISH TISSUE FILLET (FTIS)

Site ID: NRS18 	

Dale:

/

/201

T4

Length:.

999013.3

Visit#: 01 02

mm

FISH TISSUE FILLET (FTIS)
Site ID: NRS18 	

Date:

/

/201

T4

Length: _

999013,4

Visit#: 01 02
mm

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Site ID: NRS18 	

Date;

/201

Length: ^

T4

999013.5

Visit #:01 02
mm

FISH TISSUE FILLET (FTIS) - BAG
Site ID: NRS18 	

Date:

/201 Visit#: 01 02

T4

999013

B-26


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

FISH TISSUE FILLET (FTIS) - EXTRA FISH

Site ID: NRS18	

Date:	/	/201	 Visit#: Ol 02

Length:	mm

SAMPLE ID:	

FISH TISSUE FILLET (FTIS) - EXTRA FISH

Site ID: NRS18	

Date. / /201 Visit#: 01 02
Length:	mm

SAMPLE ID: 	

FISH TISSUE FILLET (FTIS) - EXTRA FISH

Site ID: NRS18	

Date: / /201 Visit#: 01 02
Length:	mm

SAMPLE ID:	

FISH TISSUE FILLET (FTIS) - EXTRA FISH
Site ID: NRS18 	

Date: / ~201 Visit#: 01 02
Length: ______ mm

SAMPLE ID: 	

FISH TISSUE FILLET (FTIS) - EXTRA FISH

Site ID: NRS18	

Date:	I	/201	Visit#: Ol 02

Length:	mm

SAMPLE ID: ,	

FISH TISSUE FILLET (FTIS) - EXTRA BAG

Site ID: NRS18	

Date:	/	/201	 Visit#: 01 02

Bag	of	

SAMPLE ID: ,	

FISH TISSUE FILLET (FTIS) - EXTRA BAG

Site ID: NRS18	

Date: / /201 Visit#: 01 02
Bag	of	

SAMPLE ID:	

FISH TISSUE FILLET (FTIS) - EXTRA FISH

Site ID: NRS18 	

Date: 7201 Visit#: Ol 02
Length:	mm

SAMPLE ID: 	

FISH TISSUE FILLET (FTIS) - EXTRA FISH

Site ID: NRS18	

Date: / /201 Visit#: 01 02
Length:	mm

SAMPLE ID: 	,

FISH TISSUE FILLET (FTIS) - EXTRA FISH

Site ID: NRS18	

Date: / /201 Visit#: 01 02

Length:	mm

SAMPLE ID: 	

FISH TISSUE FILLET (FTIS) - EXTRA FISH

Site ID: NRS18 	

Date: /' 7201 Visit#: 01 02
Length:		mm

SAMPLE ID: 	

FISH TISSUE FILLET (FTIS) - EXTRA FISH

Site ID; NRS18	

Date: / /201 Visit#: 01 02
Length: 		mm

SAMPLE ID: ,	

FISH TISSUE FILLET (FTIS) - EXTRA BAG

Site ID: NRS18	,

Date: / /201 Visit#: 01 02
Bag	of	

SAMPLE ID: 	

FISH TISSUE FILLET (FTIS) - EXTRA BAG

Site ID: NRS18	

Date: / /201 Visit#: 01 02
Bag	.of	

SAMPLE ID:	


-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

CO

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B-28

QA FISH VOUCHER (VERT)
Site ID: NRS18

	/	/ 201	

999012

QA FISH VOUCHER (VERT)
Site ID: NRS18

	/	/ 201	

999012

FISH - BAG

TAG:
41

999012

FISH - BAG

TAG:

42

999012

FISH - BAG

TAG:

43

999012

FISH - BAG

TAG:

44

999012

FISH - BAG

TAG:
37

999012

FISH - BAG

TAG:
38

999012

FISH - BAG

TAG:
39

999012

FISH - BAG

TAG:

40

999012

FISH - BAG

TAG:
33

9S9012

FISH - BAG

TAG:

34

999012

FISH - BAG

TAG:

35

999012

FISH - BAG

TAG:
36

999012

FISH - BAG

TAG:
29

999012

FISH - BAG

TAG:

30

999012

FISH - BAG

TAG:
31

999012

FISH - BAG

TAG:
32

999012

FISH - BAG

TAG:

25

999012

FISH - BAG

TAG:
26

999012

FISH - BAG

TAG:
27

999012

FISH - BAG

TAG:

28

999012

FISH - BAG

TAG;
21

999012

FISH - BAG

TAG:
22

999012

FISH - BAG

TAG:
23

999012

FISH - BAG

TAG:

24

999012

FISH - BAG

TAG:

17

999012

FISH - BAG

TAG:

18

999012

FISH - BAG

TAG:

19

999012

FISH - BAG

TAG:
20

999012

FISH - BAG

TAG:

13

999012

FISH - BAG

TAG:
14

999012

FISH - BAG

TAG:

15

999012

FISH - BAG

TAG:

16

999012

FISH - BAG

TAG:

09

999012

FISH - BAG

TAG:

10

999012

FISH - BAG

TAG:
11

999012

FISH - BAG

TAG:

12

999012

FISH - BAG

TAG:
05

999012

FISH - BAG

TAG:

06

999012

FISH - BAG

TAG:

07

999012

FISH - BAG

TAG:

08

999012

FISH - BAG

TAG:

01

999012

FISH - BAG

TAG:

02

999012

FISH - BAG

TAG:

03

999012

FISH - BAG

TAG:

04

999012


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

VOUCHER-EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #:01 02

Jar	of	

SAMPLE ID	

VOUCHER - EXTRA JAR

Site ID: NRS18	

Date: 1 /201 Visit#: 01 02

Jar	of	

SAMPLE ID	

VOUCHER - EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit#: 01 02

Jar	of	

SAMPLE ID	

VOUCHER-EXTRA JAR

Site ID: NRS18	

Date: /' /201 Visit#: 01 02

Jar	of	

SAMPLE ID	

VOUCHER - EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #: 01 02

Jar	of	

SAMPLE ID	

VOUCHER - EXTRA JAR

Site ID: NRS18	

Date:		201	 Visit#: 01 02

Jar	of	

SAMPLE ID	

VOUCHER - EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit#: 01 02

Jar	of	

SAMPLE ID	

VOUCHER-EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #: 01 02

Jar	of	

SAMPLE ID	

VOUCHER - EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #: 01 02

Jar	of	

SAMPLE ID	

VOUCHER - EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #: 01 02

Jar	of	

SAMPLE ID	

VOUCHER-EXTRA JAR

Site ID: NRS18	

Date: I /201 Visit #: 01 02

Jar	of	

SAMPLE ID	

VOUCHER - EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #: 01 02

Jar	of	

SAMPLE ID	

VOUCHER-EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #: 01 02

Jar	of	

SAMPLE ID	

VOUCHER - EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #: 01 02

Jar	of	

SAMPLE ID	


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

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Site ID; NRS18

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Site ID; NRS18

	/	/ 201	

FISH - BAG
TAG: 41

FISH - BAG
TAG:42

FISH - BAG
TAG: 43

FISH - BAG
TAG: 44

FISH - BAG
TAG: 37

FISH - BAG
TAG:38

FISH - BAG

TAG:39

FISH - BAG
TAG: 40

FISH - BAG
TAG: 33

FISH - BAG
TAG: 34

FISH - BAG
TAG: 35

FISH - BAG
TAG: 36

FISH - BAG
TAG: 29

FISH - BAG
TAG: 30

FISH - BAG

TAG: 31

FISH - BAG
TAG: 32

FISH - BAG
TAG: 25

FISH - BAG
TAG: 26

FISH - BAG
TAG; 27

FISH - BAG
TAG: 28

FISH - BAG
TAG: 21

FISH - BAG
TAG:22

FISH - BAG

TAG; 23

FISH - BAG
TAG; 24

FISH - BAG
TAG:17

FISH - BAG
TAG: 18

FISH - BAG
TAG: 19

FISH - BAG
TAG: 20

FISH - BAG
TAG: 13

FISH - BAG
TAG: 14

FISH - BAG

TAG: 15

FISH - BAG
TAG:16

FISH - BAG
TAG: 09

FISH - BAG
TAG: 10

FISH - BAG

TAG; 11

FISH - BAG
TAG: 12

FISH - BAG
TAG: 05

FISH - BAG
TAG: 06

FISH - BAG
TAG:07

FISH - BAG
TAG:08

FISH - BAG
TAG: 01

FISH - BAG
TAG: 02

FISH - BAG
TAG: 03

FISH - BAG
TAG: 04


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

FISH VOUCHER - UNK/RNG

Site ID: NRS18 	

Date:

/201 Visit#: 01 02

Jar

of

FISH VOUCHER - UNK/RNG

Site ID: NRS18 	

Date:

<201

Visit #: 01 02

Jar

of

FISH VOUCHER
Site ID: NRS18	

Date: /

UNK/RNG

Jar

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of

Visit #: 01 02

FISH VOUCHER - UNK/RNG
Site ID: NRS18	

Date: /

Jar

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of

Visit #: 01 02

FISH VOUCHER - UNK/RNG

Site ID: NRS18 	

Date:

/201 Visit #: 01 02

Jar

of

FISH VOUCHER-UNK'RNG

Site ID: NRS18 	

Date:

/201 Visit #: 01 02

Jar

of

FISH VOUCHER -

Site ID: NRS18	

Date: / /201
Jar of

UNK/RNG

Visit#: 01 02

FISH VOUCHER - UNK/RNG

Site ID: NRS18	

Date:	/

Jar

_/201	 Visit #: 01 02

of

FISH VOUCHER - UNK/RNG

Site ID: NRS18 	

Date:

	/201	

Jar	of

Visit#: 01 02

FISH VOUCHER - UNK/RNG

Site ID: NRS18 	

Date:

/201

Visit #: 01 02

Jar

of

FISH VOUCHER - UNK/RNG

Site ID: NRS18

Date:

Jar

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Visit #: 01 02

FISH VOUCHER - UNK/RNG

Site ID: NRS18

Date:

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Visit #: 01 02

FISH VOUCHER - UNK/RNG
Site ID: NRS18 	

Date:

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Visit#: CM 02

FISH VOUCHER - UNK/RNG

Site ID: NRS18 	

Date:

Jar

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of

Visit #: 01 02


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

BENTHOS COMPOSITE SAMPLE

Site ID: NRS18	

Site Name:	

Date Collected:	/	/201_

Sample Type: O BERW O BFTB

# of Transects:	

Collector(s):	

Jar	 of	

SAMPLE ID: 	

BENTHOS COMPOSITE SAMPLE

Site ID: NRS18	

Site Name:	

Date Collected:	/	/201_

Sample Type: O BERW O BETB

# of Transects:	

Collector(s):	

Jar	 of	

SAMPLE ID: 	

BENTHOS COMPOSITE SAMPLE

Site ID: NRS18	

Site Name:	

Date Collected:	/	/201_

Sample Type: O BERW O BETB

# of Transects:	

Collector(s):	

Jar	 of	

SAMPLE ID: 	

BENTHOS COMPOSITE SAMPLE

Site ID: NRS18	

Site Name:	

Date Collected:	/	/201_

Sample Type: O BERW O BETB

# of Transects:	

Collector(s):	

Jar	of	

SAMPLE ID: 	

BENTHOS COMPOSITE SAMPLE

Site ID: NRS18	

Site Name:	

Date Collected:	/	/201_

Sample Type: O BERW O BFTB

# of Transects:	

Collector(s):	

Jar	of	

SAMPLE ID: 	

BENTHOS COMPOSITE SAMPLE

Site ID: NRS18	

Site Name:	

Date Collected:	/	/201_

Sample Type: O BERW O BETB

# of Transects:	

Collector(s);	

Jar	of	

SAMPLE ID; 	

BENTHOS COMPOSITE SAMPLE

Site ID: NRS18	

Site Name:	

Date Collected;	/	/201_

Sample Type: O BERW O BETB

# of Tra nsects:	

Collector(s):	

Jar	of	

SAMPLE ID: 	

BENTHOS COMPOSITE SAMPLE

Site ID: NRS18	

Site Name:	

Date Collected:	/	/201_

Sample Type: O BERW O BETB

# of Transects:	

Collector(s):	

Jar	of	

SAMPLE ID; 	


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

BENTHOS - EXTRA JAR

Site ID: NBS18	

Date: / 7201 Visit#: 01 02

Jar	of	

SAMPLE ID: 	

BENTHOS-EXTRA JAR
Site ID: NRS18	

Date: / 7201 Visit#: 01 02

Jar	of	

SAMPLE ID: 	

BENTHOS-EXTRA JAR
Site ID: NRS18	

Date: / 7201 Visit #:01 02
Jar	of	

SAMPLE ID: 	

BENTHOS - EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #: 01 02

Jar	of	

SAMPLE ID: 	

BENTHOS-EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit#: 01 02

Jar	of	

SAMPLE ID: 	

BENTHOS-EXTRA JAR
Site ID: NRS18	

Date: / /201 Visit#: 01 02

Jar	of	

SAMPLE ID: 	

BENTHOS - EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #:01 02
Jar	of	

SAMPLE ID: 	

BENTHOS - EXTRA JAR

Site ID: NRS18	

Date: 7 /201 Visit #: 01 02

Jar	of	

SAMPLE ID: 	

BENTHOS-EXTRA JAR

Site ID: NRS18	

Date: 7 /201 Visit #: 01 02

Jar	of	

SAMPLE ID: 	

BENTHOS-EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #: 01 02
Jar	of	

SAMPLE ID: 	

BENTHOS-EXTRA JAR

Site ID: NRS18	

Date: 7 7201 Visit #: 01 02
Jar	of	

SAMPLE ID: 	

BENTHOS - EXTRA JAR
Site ID: NRS18	

Date: 7 7201 Visit #: 01 02
Jar	of	

SAMPLE ID: 	

BENTHOS - EXTRA JAR

Site ID: NRS18	

Date: / /201 Visit #: 01 02
Jar	of	

SAMPLE ID: 	

BENTHOS-EXTRA JAR

Site ID: NRS18	

Date: / 201 Vi t# 01 02
Jar	or	

SAMPLE ID: 	


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Appendix C: Shipping
Guidelines

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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

General Shipping Guidelines

Samples will be shipped according to the chart below (Table C.2). The Field Crew Leader will complete
the appropriate sample information and tracking form for the samples and will submit tracking
information via the NRSA App (alternative methods are listed below). The Field Crew Leader will place
the samples and a packing slip (in a waterproof bag or plastic sleeve) in the shipment cooler, and then
the Field Crew Leader will attach the appropriate pre-addressed FedEx label for the appropriate lab. The
field crew will either drop off the cooler for shipment at a local FedEx location or arrange for a pick up at
the hotel or other appropriate facility. If the field crew has chosen a pick up, they must follow up with
the facility at which it has been left and/or track the package through FedEx tracking tools to ensure that
the shipment cooler has been picked up by FedEx. Once the package is in the possession of FedEx, the
IM Team and Field Logistics Coordinator (FLC) will track the package to its destination and take steps
necessary to ensure timely delivery.

Tracking information must be submitted electronically to the IM Team and a packing slip or tracking
form must accompany each sample shipment. The packing slip or tracking form is to be placed in a
resealable plastic bag/pouch secured to the inside of the cooler lid. Seal the shipping container. Submit
the sample tracking information to the NARS IM Team to indicate that samples will be in transit to the
laboratory. Tracking information must be submitted the same day that the samples are shipped.

Before shipping, it is very important to preserve each sample as directed in the sample collection
portion of the appropriate chapter in the NRSA 2018-19 FOM. General directions for sample processing,
shipping and tracking are found below:

•	Preserve the samples as specified for each indicator before shipping.

•	Be aware of the holding times for each type of sample.

•	Always line the cooler with a heavy duty plastic bag (cooler liner) when shipping with wet ice or
when shipping preserved samples.

•	When shipping samples preserved with ethanol, surround the jars with crumpled newspaper or
other absorbent material.

When wet ice is used for shipment:

•	Ensure that the ice is fresh before shipment, and use adequate amounts of ice to ensure
samples will remain cold for up to 36 hours.

•	Place samples and ice inside the cooler liner and seal the cooler liner.

•	Secure the cooler with packing tape.


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National Rivers and Streams Assessment 2018/19

Field Operations Manual

Version 1.2 May 2018

When dry ice is used for shipment:

•	When shipping frozen batched samples, place the dry ice pad in the bottom of the cooler and
then line the cooler with the dry ice liner (silver bubble material).

•	Ship frozen batched samples with 20 pounds of dry ice.

•	Ship whole fish tissue samples with 50 pounds of dry ice.

•	When shipping whole fish tissue or frozen batched samples, place a layer of dry ice in the
bottom of the cooler, samples in a middle layer, and a second layer of dry ice on top of the
samples.

•	Affix a Class 9 Hazardous Goods label (Figure C.l) to the outside of the cooler making sure that it
indicates the sender, recipient and amount of dry ice (in kg).

Figure C.l Class 9 Hazardous Goods label for use with dry ice shipments

NOTE: Not all FedEx locations will accept shipments containing dry ice. Dry ice shipments can be shipped
from "FedEx staffed" locations. You can also arrange for a pick-up from your lab or hotel. Dry ice
shipments often cannot be shipped from FedEx Office and Print Centers® or FedEx Authorized
ShipCenter® locations. These types of locations are differentiated on FedEX.com in the "Find FedEx
Locations" feature. Please be sure to call in advance to ensure your location will accept the package for
shipment.

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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

When	shipping samples preserved with ETOH or Formalin (non-chilled batched samples):

•	Close the lid of each container tightly and securely with electrical tape

•	Line the cooler with the provided cooler liner.

•	Place sealed sample containers inside cooler liner

•	Surround the jars with crumpled newspaper, pads, or other absorbent material

•	Seal the cooler liner securely with zip tie.

•	Secure the cooler with packing tape.

NOTE: Federal regulations and FedEx rules allow for Ground shipping of certain quantities of flammable
liquids WITHOUT the need for special certifications and labeling. Flammable liquids may NOT be shipped
via air carrier unless shipper is trained and qualified to do so and specific documentation and labeling
requirements are met

The Code of Federal Regulations (49 CFR Section 173.150) lists the exceptions which allow shipping of
flammable liquids via ground carrier without labeling or special certifications. Ethanol and formalin can
be considered to be in either Packaging Group 2 or 3, so we use the more stringent PG 2 as our guideline.
The limited quantity exclusion allows ground shipping ofPG 2 flammable liquids provided that the
individual containers inside the package are not over 1.0 liters each, that the gross weight of the package
does not exceed 66 pounds, and that the outer packaging is a sturdy container. Please ensure that your
shipment meets these criteria to ensure the legal Ground shipment of these samples.

Tracking Forms and Shipment Types

Whenever NRSA samples are shipped, one or more tracking forms are completed to relay important
shipping information to the IM Team, FLC and the destination lab. These tracking forms are to be
submitted both electronically at the time of shipping (through app submission or by emailing a scan of a
paper version of the form) and included as a hard copy (packing slip or tracking form) in the shipping
container with the samples.

Packing Slips

Packing slips (Figure C.2) are postcard sized slips that accompany sample labels in the site kits. When
crews order site kits (via the Request Form), a set of sample labels and packing slips will accompany
each site kit. Sample IDs for the suite of samples collected at a single site will be pre-populated on both
the labels and the packing slips. It is important to keep the labels and packing slips organized so the
sample IDs will match when shipping occurs. It is a good idea to write the Site ID in the header area of all
packing slips when preparing the Site Packet, even if the slip will not be used to ship samples right away.
This will help ensure that the sample IDs do not get mis-matched.

When crews submit tracking data via the app, a tracking form will be populated and sent back to them
as a PDF document that will look very much like a paper tracking form (see below). Crews DO NOT need
to print this form as all tracking information is submitted electronically and the packing slip acts as the
chain of custody for the samples in the cooler. The returned PDF is simply a record of the submission for
the crew to keep.


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

T2: NR5A18/19 frozen Batches * a eking

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Paper Tracking Forms

Crews who are NOT using the NRSA App (E-Forms) for data submission will need to order paper data
forms and paper tracking forms (Figure C.3) for each site. Labels will still be sent in the site kits and the
crew will need to carefully transfer sample IDs to the paper tracking forms. Crews who are sending a
subset of samples to a state lab may also need paper tracking forms or can use a fillable PDF tracking
form.

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Site ID; NRS18	Visit: O'l 0 2

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8670482410

Tracking Related Inquiries:

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Figure C.3. Example of a T2 Paper Tracking form

C-6


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National Rivers and Streams Assessment 2018/19
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Field Operations Manual

Each packing slip and tracking form has been assigned a "T" number to help crews identify the correct
slip/form to use when sending samples. This "T" number is located on the top of each slip/form in the
title of the form (e.g., Tl: NRSA18/19 Daily Water Chemistry Sample Tracking). Crews will also find
reference to the same "T" numbers on the individual sample labels and on the top of the pre-printed
FedEx return labels provided in the coolers. The FedEx return labels are pre-paid and allow crews to ship
samples to any of the nationally contracted laboratories. States using their own labs for certain samples
will need to arrange for shipping on their own.

Crews include copies of all packing slips or tracking forms in the coolers (placed in the plastic sleeve
affixed to the inside of the cooler lid) when they send samples to the labs, and they also must submit an
electronic copy to the IM Team. Crews have several different options for electronically submitting
sample and tracking information. If a cooler contains samples from more than one site, then multiple
slips/forms must be placed in the cooler and submitted to NARS IM.

In order of preference, the options for submitting sample and tracking information are:

1.	Using the NRSA App:

a.	Enter all applicable sample data in the pertinent sections of the NRSA App. For any
targeted sample not collected, be sure to indicate a reason why the sample was not
collected in the comment bubble adjacent to the "no sample collected" check box.

b.	Fill out the correct tracking form in the app with shipping information (e.g., lab, ship
date, airbill number, sender name, and sender phone number).

c.	Submit the tracking form via the app.

d.	On the pre-populated packing slip that accompanied the sample labels, enter the Site
ID, Visit number, and Date collected.

e.	For any sample not included in the cooler, line out the sample ID.

f.	Insert the packing slip into the plastic sleeve on the inside of the cooler lid, seal the
cooler with packing tape, apply the correct FedEx shipping label, and ship the cooler.

2.	Using a paper tracking form:

a.	Hand-enter sample IDs and complete tracking data on a paper tracking form.

b.	Scan the form with a handheld device or office scanner. Attach the file (in PDF version)
to an email and address to sampletracking@epa.gov. Be sure that the file scanned is
clear and legible. If an office scanner is not available at the time of shipping, consider a
free application like CamScanner or AdobeScan which is widely available for mobile
devices and has processing tools that will help to ensure that the scan is clear and
legible.

• Please name the PDF files in the following format: "SitelD V# T#", where:

¦	'SitelD' is the NRSA18-19 Site ID as listed in the Site Evaluation
Spreadsheet (prefix may be omitted);

¦	'V#' is the visit number (e.g., VI or V2); and

¦	'T#' is the number of the tracking form.

• Example: AL_10001 VI Tl

¦	The specified naming convention is provided on the bottom of each form
as a reminder.

c.	After scanning, insert the tracking form into the plastic sleeve on the inside of the cooler
lid, seal the cooler with packing tape, apply the correct FedEx shipping label, and ship
the cooler.

3.	Using a fillable PDF tracking form (may be best for state lab use):

a. Using a handheld device or portable computer, enter sample IDs and complete tracking
data into a fillable PDF tracking form, save and submit it via email. Fillable PDF tracking


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

forms do not have pre-populated sample IDs, so care must be taken to enter all sample
IDs exactly as they are displayed on the sample labels. The fillable PDFs must be saved
as an updated copy to the user's computer or device and then attached to an email to
sampletracking@epa.gov.

• Please name the PDF files in the following format: "SitelD V# T#", where:

¦	'SitelD' is the NRSA18-19 Site ID as listed in the Site Evaluation
Spreadsheet (prefix may be omitted);

¦	'V#' is the visit number (e.g., VI or V2); and

¦	'T#' is the number of the tracking form.

• Example: ALJ.0001 VIT1

¦	The specified naming convention is provided on the bottom of each form
as a reminder.

b. Print the completed PDF and include the hard copy of the form in the cooler with the
samples. This step may be omitted for coolers shipped to state labs if the lab does not
require the receipt of this tracking form. Insert the tracking form into the plastic sleeve
on the inside of the cooler lid, seal the cooler with packing tape, apply the correct FedEx
shipping label, and ship the cooler.

Status Reports:

When a crew samples a site, they complete and submit a copy of the T1 - Site & Sample Status/Water
Chemistry Lab Tracking Form. This will typically be done via the NRSA App but can be accomplished
through the use of a paper or fillable PDF version. It is very important to submit this form as soon as
possible after every sampling event. Prompt status reports allow the FLC to closely track sampling
progress. More importantly, it enables NARS IM to track samples that were collected at each
successfully sampled site versus those that were not, and to immediately track the shipment of the
time-sensitive samples after each sampling event.

Daily Shipments:

T1 - Site & Sample Status/Water Chemistry Lab Tracking Form

•	Complete Form Tl: Site and Sample Status/Water Chemistry Lab Tracking Form as

soon as possible after completing a sampling event.

•	Complete the header area to indicate site-specific information (see Figure C.4, Section
A). For App users, this information is contained in the Verification Form.

•	Complete the Site Status section (Figure C.4, Section B) in all cases to indicate whether
the site was sampled or not and the method used to sample. For App users, this
information is contained in the Verification Form.

•	Complete the shipment details section (Figure C.4, Section C) to indicate date shipped,
airbill number, and sender info. For App users, this information is completed in the Tl
Tracking Form.

•	Complete the immediately shipped sample section (Figure C.4, Section D) to indicate
sample IDs, lab, and reasons that targeted samples were not collected. For App users,
this information is contained in the pertinent sample collection forms. Be sure to indicate
a reason why a targeted sample was not collected in the comment bubble adjacent to
the "no sample collected" check box in the app forms.


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Field Operations Manual

•	In cases where a state lab is processing some, but not all of the samples, one copy of the
T1 form will be filled out for each lab and submitted separately. The T1 packing slip or
tracking form placed in the cooler should only indicate the sample(s) being shipped in
that cooler.

•	Packing slips are to be used when samples are sent to national labs. For any sample
not included in the cooler, line out the sample ID.

•	Paper or PDF tracking forms are to be used for state labs. Use the 'included in
cooler' bubble to indicate which samples are being shipped with the specific form.

•	See Table C.l for guidance on when to use packing slips versus paper or PDF
tracking forms.

•	Complete the Batched Sample Status section (Figure C.4, Section E) to indicate whether
each type of sample was collected. If any of the samples were targeted but not
collected, use the comments section to briefly explain why they were not collected. For
App users, this information is contained in the pertinent sample collection forms. Be sure
to indicate a reason why a targeted sample was not collected in the comment bubble
adjacent to the "no sample collected" check box in the app forms.

•	Indicate how many Enterococci filters were collected (typically 2 at normal
visits, 3 at Visit 1 to revisit sites).

•	Indicate how many jars of each type were collected (in the case of benthos
and fish voucher samples).

•	Complete the Field Data Submission section (Figure C.4, Section F) to indicate how field
data will be submitted (Electronic App, Fillable PDF, Paper Forms, or Partial App/Paper).
For App users, this information is automatically populated.

•	Send an electronic copy of this form to NARS IM using one of the options listed above.
This serves as the "status report" for that sampling event.


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National Rivers and Streams Assessment 2018/19
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Field Operations Manual

Section A

Section B

Section C

Section D

Section E

Section F

FORM T1: NRSA18/19 SITE AND SAMPLE STATUS/WATER CHEMISTRY LAB TRACKING |

¦«* IS: NRS18,	 v«rt »: o 1 O 2

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Stale of Sit* Location

Oat* Collected

/,

Site Status - Is Site Sampleable?
O YES - Check one below

O Wadeable - Continuous watt? gmter than 50 . wadeabi®
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O Partial • Samples By waaitg i -50 of reach sampled) Explain
O Pa'tiai • Sampled bv boat r-W < of reach iampiedi Explain hi
O Wadeable Interrupted Not continuous «at#r along reach
O Soatsbi# interrupted • Not continuous *at« along reach
O Altered • Streair River Channel Present but d«ers from Map

Sample Status - Immediate Shipped Samples

snipped by O ! > O 1
Airbill Tracking Number

O NO * Complete Verification (using app or paper)
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Phone e4" -754-446?

' Emai tc sampletrackmgsgepa gov Michelle Cover

Phone 541 •"54-4793
I	i Chris Turner

'	Phc^e "7!5-S:9-3"37

Figure C.4 Example of Tracking Form Tl: Site and Sample Status/Water Chemistry Lab Tracking

Batched Sample Shipments:

•	Crews may hold batched shipments for the amount of specified in the table below.

•	Electronically send the tracking form(s) to NARS IM when the samples are SHIPPED using
one of the options listed above.

•	Use one packing slip or tracking form for each site's worth of samples in the cooler (i.e.
if you have batched samples from four sites in the cooler, there should be four forms
completed).

•	Include paper copies of the packing slips or tracking forms in the cooler.

•	All samples in the cooler should be listed on one of the included tracking forms.

T2 - NRSA 2018-19 Frozen Batched Samples Tracking

•	Use this form when shipping frozen batched samples (Enterococci, fish plugs, algal
toxins, and periphyton DNA samples). These samples should be shipped within one
week of collection.

•	For App users, the bulk of the sample information is contained in the pertinent sample
collection forms within the app. Shipping information is entered into the T2 Tracking
Form.


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•	Paper form or fillable PDF Form users must transfer sample IDs from the sample labels
to the tracking form.

•	In cases where a state lab is processing some, but not all of the samples, one copy of the
T2 form will be filled out for each lab and submitted separately. The T2 packing slip or
tracking form placed in the cooler should only indicate the sample(s) being shipped in
that cooler.

•	Packing slips are to be used when samples are sent to national labs. For any sample
not included in the cooler, line out the sample ID.

•	Paper or PDF tracking forms are to be used for state labs. Use the 'included in
cooler' bubble to indicate which samples are being shipped with the specific form.

•	See Table C.l for guidance on when to use packing slips versus paper or PDF
tracking forms.

•	Complete the header area to indicate site-specific information and shipping information.

•	Write in or verify the sample IDs for the sample(s) being shipped.

•	Indicate to which laboratory the samples are being shipped (i.e., national or state
laboratory). If shipping to a state laboratory, provide the laboratory name.

•	Send an electronic copy of this form to NARS IM using one of the options listed above.

•	Ship the samples to the lab(s) in a cooler with a hard copy of the respective slip/form.

T3 - NRSA 2018-19 Non-Chilled Batched Samples Tracking

•	Use this form for shipping batches of non-chilled samples (benthos, periphyton ID
samples, and fish voucher samples).

•	These samples are shipped together in a cooler (ordered via the Request form) with a
heavy duty cooler liner and no ice. Benthos and periphyton ID Samples should be
shipped within two weeks of collection. Fish voucher samples may be shipped after fish
are fixed, preserved and assembled into the voucher collection with voucher tags.

•	The number of samples that will fit in a cooler will depend largely on the number of
benthos bottles collected from each site. In most cases, samples from three to five site
visits will fit in a cooler together. NEVER split samples from one site into more than one
cooler.

•	In cases where a state lab is processing some, but not all of the samples, one copy of the
T3 form will be filled out for each lab and submitted separately. The T3 packing slip or
tracking form placed in the cooler should only indicate the sample(s) being shipped in
that cooler.

•	Packing slips are to be used when samples are sent to national labs. For any sample
not included in the cooler, line out the sample ID.

•	Paper or PDF tracking forms are to be used for state labs. Use the 'included in
cooler' bubble to indicate which samples are being shipped with the specific form.

•	See Table C.l for guidance on when to use packing slips versus paper or PDF
tracking forms.

•	Complete the header area to indicate site-specific information and shipping information.

•	Write in or verify the sample IDs for the sample(s) being shipped.

•	Indicate to which laboratory the samples are being shipped (i.e., national or state
laboratory). If shipping to a state laboratory, provide the laboratory name.


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•	Send an electronic copy of this form to NARS IM using one of the options listed above.

•	Ship the samples to the lab(s) in a cooler with a hard copy of the respective slip/form.

•	Batched shipped samples need to be shipped with absorbent material and NO ice.

•	Place 1-liter bottles upright in the lined cooler and use newspaper or cardboard to fill
any empty space between benthos bottles to keep them in an upright position during
shipping.

T4 - NRSA 2018-19 Whole Fish Tissue Samples Tracking

•	Use this form when shipping frozen whole fish tissue samples. These samples should be
shipped within two weeks of collection.

•	For App users, the bulk of the sample information is contained in the pertinent sample
collection forms within the app. Shipping information is entered into the T4 Tracking
Form.

•	Paper form or fillable PDF Form users must transfer sample IDs from the sample labels
to the tracking form and must also populate the fish common name and lengths of each
specimen.

•	Complete the header area to indicate site-specific information and shipping information.

•	Write in or verify the sample IDs for the sample(s) being shipped.

•	Send an electronic copy of this form to NARS IM using one of the options listed above.

•	Ship the samples to the lab in a cooler with a hard copy of the respective slip/form.

T5 - NRSA 2018-19 Pack Tracking

•	If utilizing paper field forms, review and ship all field forms in the envelope provided in
the site kit to NARS IM every two weeks.

•	Before shipping, make copies or scans for your records and as a backup in the event the
forms are lost during shipping.

•	Forms should be sequentially ordered by protocol within the Data Packet to aid in
determining that all forms are present and to facilitate data entry once the Data Packet
reaches the IM Team.

•	The T5 form is the only tracking form which has room for multiple sites to be listed. List
the site IDs, dates sampled and visit numbers for all data packets being shipped in the
envelope or box.

•	You do not need to send multiple scans of the same file.

•	You can name the file with a composite name such as "AL_10001 VI, AL_10022 VI,
AL_10235 V2, AL_11236 VI T5".


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Table C.l. Guidance on using packing slips and tracking forms



Samples in the shipping
group (Tl, T2, T3) are all
going to the national lab

Submit tracking via the NRSA App, complete a packing slip, and
include the packing slip in the cooler.



Samples in the shipping
group (Tl, T2, T3) are all
going to a state lab

Submit tracking via the NRSA App (indicating name of state lab),
complete a packing slip, and include the packing slip in the cooler*.

Site data was
collected using
NRSA APP

Samples in the shipping
group (Tl, T2, T3) are
being split between the
national lab and state
lab(s)

•	For samples going to the national lab, submit tracking via the
NRSA App (checking ONLY the samples going to the national lab),
complete a packing slip (lining out the sample IDs for samples
not shipped to the national lab), and include the packing slip in
the cooler.

•	For samples going to state lab(s), complete a PDF tracking form
for each lab (indicating only the samples included in each
cooler), and email the form to sampletracking@epa.gov. Include
the paper form in the cooler*



Samples in the shipping
group (Tl, T2, T3) are all
going to the national lab

Complete a paper tracking form, scan and email the form to
sampletracking@epa.gov, and include the paper form in the cooler



Samples in the shipping
group (Tl, T2, T3) are all
going to a state lab

Complete a paper or fillable PDF tracking form, scan and email the
form to sampletracking@epa.gov, and include the paper form in
the cooler*

Site data was
collected using
paper forms

Samples in the shipping
group (Tl, T2, T3) are
being split between the
national lab and state
lab(s)

•	For samples going to the national lab, complete a paper tracking
form (indicating only the samples included in the cooler), scan
and email the form to sampletracking@epa.gov, and include the
paper form in the cooler

•	For samples going to state lab(s), complete a PDF tracking form
for each lab (indicating only the samples included in each
cooler), and email the form to sampletracking@epa.gov. Include
the paper form in the cooler*

* If state labs do not request the NRSA packing slip or tracking form be placed in the cooler being shipped
to them, that step may be omitted.


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SAMPLE-SPECIFIC SHIPPING GUIDELINES

Before shipping, it is very important to preserve each sample as directed in the sample collection
portion of this Field Operations Manual.

¦	Preserve the samples as specified for each indicator before shipping (Fig. C.5).

¦	Be aware of the holding times for each type of sample (Table C.2):

o Water chemistry samples must be shipped within 24 hours of collection (e.g., the

same day as sampling or the following day),
o Chlorophyll-o and periphyton biomass samples have longer holding times, but will be

sent with the water chemistry samples since they are going to the same laboratory,
o Enterococci samples must be filtered and frozen on dry ice within 6 hours of

collection and must be shipped within one week of collection,
o Fish tissue samples (plugs and whole fish) must be frozen on dry ice as soon as

possible (hold on wet ice until freezing on dry ice if needed),
o Microcystin samples should be held on wet ice in the field and frozen upon return to
lab, office or hotel.

o Fish voucher specimens can be held on wet ice until being preserved in formalin in

the field or laboratory. Preserve samples ASAP,
o The remaining samples must be preserved immediately upon collection; they may
then be sent in batches to the appropriate laboratory.

WATER CHEMISTRY, CHLOROPHYLL-a (from Water Column), Chlorophyll-a (from
periphyton) and Periphyton Biomass
o Water Chemistry

Stored in a 4-L cube container

¦	Confirm that the cube container is labeled and covered with clear tape.

¦	Ensure that air is purged from the container

¦	Seal lid with electrical tape

¦	Place the cube container inside the cooler liner.

o Water Column Chlorophyll-a, Periphyton Chlorophyll-a and Periphyton Biomass

Three filters each stored in a 50-mL centrifuge tube wrapped with aluminum foil

Filters are initially frozen on dry ice in the field if possible, then shipped on wet ice to WRS lab or

approved State lab.

¦	Confirm that the labels with sample IDs are completed (including volume of sample

filtered) and covered with clear tape.

¦	Close lids tightly (turn an additional / turn past the point at which initial resistance is met)

¦	Seal lids with electrical tape

¦	Cover the two cholorphyll filter tubes with aluminum foil

¦	Place the three centrifuge tubes in the provided leak-proof zip top plastic bag.

¦	Label the bag with the appropriate outer bag label.

¦	Place bagged samples inside cooler liner with water chemistry sample.

PERIPHYTON ID SAMPLES

ID samples preserved with formalin and sealed at the site.

¦	Confirm that the label with sample ID is completed and covered with clear tape.

¦	Close lids tightly (turn an additional % turn past the point at which initial resistance is met)

¦	Seal lids with electrical tape


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¦	Place the sample in the appropriate shipping container with other preserved samples.

¦	Surround the jars with crumpled newspaper, pads, or other absorbent material.

¦	Samples can be held for up to two weeks and shipped in batches to the laboratory for

analysis.

BENTHIC INVERTEBRATE SAMPLES

Preserved in 95% ethanol and sealed at the site.

¦	Confirm that the label with sample ID is completed and covered with clear tape.

¦	Confirm that each jar contains a completed inner label.

¦	Check to make sure jars are sealed with electrical tape.

¦	Place3-5 sites worth of jars in each cooler with other preserved samples.

¦	Surround the jars with crumpled newspaper, pads, or other absorbent material.

¦	Samples can be held for up to two weeks and shipped in batches to the laboratory for

analysis.

WHOLE FISH TISSUE SAMPLES

The samples need to be frozen as soon as possible after collection.

¦	Pack the provided cooler with 50 lbs of dry ice.

¦	Refer to the DRY ICE SHIPPING PROTOCOLS at the beginning of this Appendix.

¦	Samples may be stored on dry ice for a maximum of 24 hours. Sampling crews have the

option, depending on site logistics, of:
o shipping the samples packed on dry ice (50 pounds), via priority overnight delivery so
that they arrive at the designated laboratory within 24 hours of sample collection
(Microbac Laboratories in Baltimore, MD), or
o freezing the samples within 24 hours of collection at <-20°C, and storing the frozen
samples until shipment within 2 weeks of sample collection (frozen samples will be
packed on dry ice and shipped to Microbac Laboratories via priority overnight delivery
service).

o Microbac does not accept Saturday deliveries, so shipment must take place Monday
through Thursday only.

FISH VOUCHER SAMPLES

Fixed in the field with formalin, preserved in a laboratory with ethanol.

¦	Package each fish species according to fish OA vouchering protocol.

¦	Confirm that the label with sample ID is completed and covered with clear tape.

¦	Check to make sure jars are sealed with electrical tape.

¦	Surround the jars with crumpled newspaper, pads, or other absorbent material.

¦	Samples can be held and shipped in batches to the laboratory for analysis.

ENTEROCOCCI SAMPLES

The sample needs to be filtered and frozen as soon as possible after collection (within 6 hours).

¦	Confirm that each microcentrifuge tube is labeled (DO NOT cover labels with tape) and

properly sealed (DO NOT seal caps with tape).

¦	Place microcentrifuge tubes from a single site in a padded bag.

¦	Confirm that the bag is labeled with the appropriate sample ID (DO NOT cover with clear

plastic tape).

¦	Place the samples in cooler with dry ice liner and pad with other frozen batched samples.

¦	Pack the box with 20 lbs of dry ice.

¦	Refer to the DRY ICE SHIPPING PROTOCOLS at the beginning of this Appendix.


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¦	Samples can be held frozen and shipped in batches to the laboratory for analysis within one

week of collection.

FISH PLUG SAMPLES

The samples need to be frozen as soon as possible after collection.

¦	Confirm that the label with sample ID is completed and covered with clear tape.

¦	Check to make sure vial is sealed with electrical tape.

¦	Refer to the DRY ICE SHIPPING PROTOCOLS at the begining of this Appendix.

¦	Place the samples in cooler with dry ice liner and pad with other frozen batched samples.

¦	Pack the box with 20 lbs of dry.

¦	Refer to the DRY ICE SHIPPING PROTOCOLS at the beginning of this Appendix.

¦	Samples can be held frozen and shipped in batches to the laboratory for analysis within one

week of collection.

ALGAL TOXIN SAMPLES

The samples need to be frozen as soon as possible after collection.

¦	Confirm that the label with sample ID is completed and covered with clear tape.

¦	Check to make sure bottle is sealed with electrical tape.

¦	Refer to the DRY ICE SHIPPING PROTOCOLS at the beginning of this Appendix.

¦	Place the samples in cooler with dry ice liner and pad with other frozen batched samples.

¦	Pack the box with 20 lbs of dry ice.

¦	Refer to the DRY ICE SHIPPING PROTOCOLS at the beginning of this Appendix.

¦	Samples can be held frozen and shipped in batches to the laboratory for analysis within one

week of collection.

PERIPHYTON METAGENOMICS (DNA) SAMPLES

The samples need to be frozen as soon as possible after collection.

¦	Confirm that the label with sample ID is completed and covered with clear tape.

¦	Check to make sure bottle is sealed with electrical tape.

¦	Refer to the DRY ICE SHIPPING PROTOCOLS at the beginning of this Appendix.

¦	Place the samples in cooler with dry ice liner and pad with other frozen batched samples.

¦	Pack the box with 20 lbs of dry ice.

¦	Samples can be held frozen and shipped in batches to the laboratory for analysis within one

week of collection.


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NRSA 2018-2019 Shipping Summary



T1 - Water
Chemistry Samples

(1) Cubitainer(4 L)

CHEM
(3) filters in
centrifugetubes
(50 mL), in zip-top
bag
WCHL
PCHL

PBIO



J

s

( T2



T2 - Frozen Samples (Batched) - per
site

(1) PETG bottle(500mL,clear,square)
MICX

(1)	HOPE bottle(500 mL, white, round)

MICZ

(2)	Filters in microcentrifugetubesinbag

(plus blank if revisit site VI)

ENTE

(2) FishTissuePlugsinlvial
FPLG

(1) PETG bottle(125 mL)

PDNA

T3 - Non-Chilled Samples
(Batched) - per site

(1 or more) 1L wide-mouth
bottle
8ERW (wadeable)-or-
BETB (non-wadeable)
(1) Centrifugetube(screw-top,
50-mL)

PERI

(1 or more) 1 Lwide-mouth
bottle

VERT (or voucher sites only)

Frozen Whole

Fish Tissue
Samples - per
site

(5) FishinLarge
plastic
(composite)
bag(s)

FTIS

At selected sites
only

I

T





Completed
Data Packs
(Batched) For
Paper Forms Only

All data forms
from a site,
reviewed for
completeness,
legibility and
accuracy

I



I

Pack 1 site's worth
of samples in lined
coolerwithwetice

Pack 3-5 site's worth of samples in
cooler lined with dry ice liner and
pad and with 20 lbs dry ice

I

Pack 3-5 site's worth of
samples in lined coolerwith
NO ICE





I

Pack 1 sample in
coolerwith
50 lbs dry ice

SHIPWITHIN 24
HOURS
(MON-FRI)



Express
PRIORITY
OVERNIGHT

SHIP WEEKLY
(MON-FRI)

Express

PRIORITY OVERNIGHT

SHIP EVERY 2 WEEKS
(ANYTIME)

I

Make copies or scars
as backup

Organize asspecified
in the FOM

Place in envelope
withtrackingform



Ground

GROUND

SHIP
MON-THURS

(NO Saturday)
Delivery)

Express
PRIORITY
OVERNIGHT

* Whole fish samples are collected at a subset of pre-selected sites
Figure C.5. Sample packaging and shipping summary


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Table C.2 Sample preservation and holding times

SAMPLE

PRESERVATIVE

PACKAGING FOR
SHIPMENT

HOLDING TIME

T1

WATER CHEMISTRY
SAMPLES

Water Chemistry
CHEM

Wet ice

Ship in lined
cooler with wet
ice

24 hours; ship these
samples together
(WRS lab - Corvallis
or State approved lab

Chlorophyll-o
WCHL

Dry ice in field

Periphyton - Chlorophyll-o
PCHL

Dry ice in field

Periphyton - Biomass
PBIO

Dry ice in field

T2

FROZENBATCHED SAMPLES

Algal Toxins (2)

MICX

MICZ

Wet ice in field,
Freeze as soon as
possible

Ship in cooler
with provided dry
ice liner and pad
with 20 pounds of
DRY ICE

Batch; ship weekly to
GLEC lab Batch

Enterococci
ENTE

Dry ice in field; MUST be
filtered & frozen within
6 hours of collection
Hold in freezer or on dry
ice

Fish Tissue Plugs
FPLG

Dry ice in field; Hold in
freezer or on dry ice

Periphyton Metagenomics
PDNA

Wet ice in field, freeze
as soon as possible

T3

NON-CHILLED BATCHED
SAMPLES

Periphyton - ID
PERI

Formalin

Ship in lined
cooler

Batch; ship at least
every 2 weeks to
GLEC

Benthic

macroinvertebrates
BERW or BETB

95% Ethanol

Fish Vouchers*
VERT

95% Ethanol

T4

WHOLE FISH
TISSUE

Whole Fish Tissue*
FTIS

Dry ice in field; Hold in
freezer

Ship in provided
cooler with 50
pounds of
DRY ICE

If holding in a freezer
ship within 2 weeks
to Microbac lab

*Fish vouchers and whole fish tissue are collected at selected site only.


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Appendix D: Standard
Common and Scientific
Names of Fish and
Amphibians


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Table D-l. Standard Common and Scientific Names of Fish

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Acipenseriformes

Acipenseridae

Acipenser oxyrinchus

atlantic sturgeon

Acipenseriformes

Acipenseridae

Acipenser medirostris

green sturgeon

Acipenseriformes

Acipenseridae

Acipenser fulvescens

lake sturgeon

Acipenseriformes

Acipenseridae

Acipenser brevirostrum

shortnose sturgeon

Acipenseriformes

Acipenseridae

Acipenser transmontanus

white sturgeon

Acipenseriformes

Acipenseridae

Scaphirhynchus suttkusi

alabama sturgeon

Acipenseriformes

Acipenseridae

Scaphirhynchus albus

pallid sturgeon

Acipenseriformes

Acipenseridae

Scaphirhynchus platorynchus

shovelnose sturgeon

Acipenseriformes

Polyodontidae

Polyodon spathula

paddlefish

Amiiformes

Amiidae

Amia calva

bowfin

Anguilliformes

Anguillidae

Anguilla rostrata

american eel

Anguilliformes

Congridae

Conger oceanicus

conger eel

Anguilliformes

Ophichthidae

Myrophis punctatus

speckled worm eel

Atheriniformes

Atherinopsidae

Labidesthes sicculus

brook silverside

Atheriniformes

Atherinopsidae

Membras martinica

rough silverside

Atheriniformes

Atherinopsidae

Menidia beryllina

inland silverside

Atheriniformes

Atherinopsidae

Menidia audens

mississippi silverside

Atheriniformes

Atherinopsidae

Menidia extensa

waccamaw silverside

Atheriniformes

Atherinopsidae

Menidia menidia

atlantic silverside

Beloniformes

Belonidae

Strongylura marina

atlantic needlefish

Carcharhiniformes

Carcharhinidae

Carcharhinus leucas

bull shark

Characiformes

Characidae

Astyanax mexicanus

mexican tetra

Clupeiformes

Clupeidae

Alosa alabamae

alabama shad

Clupeiformes

Clupeidae

Alosa pseudoharengus

alewife

Clupeiformes

Clupeidae

Alosa sapidissima

american shad

Clupeiformes

Clupeidae

Alosa aestivalis

blueback herring

Clupeiformes

Clupeidae

Alosa mediocris

hickory shad

Clupeiformes

Clupeidae

Alosa chrysochloris

skipjack herring

Clupeiformes

Clupeidae

Dorosoma cepedianum

gizzard shad

Clupeiformes

Clupeidae

Dorosoma petenense

threadfin shad

Clupeiformes

Clupeidae

Harengula jaguana

scaled sardine

Clupeiformes

Clupeidae

Opisthonema oglinum

atlantic thread herring

Clupeiformes

Clupeidae

Brevoortia patronus

gulf menhaden

Clupeiformes

Engraulidae

Anchoa mitchilli

bay anchovy

Cypriniformes

Catostomidae

Carpiodes velifer

highfin carpsucker

Cypriniformes

Catostomidae

Car pi odes cyprinus

quillback

Cypriniformes

Catostomidae

Carpiodes carpio

river carpsucker

Cypriniformes

Catostomidae

Catostomus discobolus

bluehead sucker

Cypriniformes

Catostomidae

Catostomus columbianus

bridgelip sucker

Cypriniformes

Catostomidae

Catostomus clarkii

desert sucker


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ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Cypr

ni

ormes

Catostomidae

Catostomus latipinnis

flannelmouth sucker

Cypr

ni

ormes

Catostomidae

Catostomus snyderi

klamath largescale sucker

Cypr

ni

ormes

Catostomidae

Catostomus rimiculus

klamath smallscale sucker

Cypr

ni

ormes

Catostomidae

Catostomus macrocheilus

largescale sucker

Cypr

ni

ormes

Catostomidae

Catostomus catostomus

longnose sucker

Cypr

ni

ormes

Catostomidae

Catostomus microps

modoc sucker

Cypr

ni

ormes

Catostomidae

Catostomus platyrhynchus

mountain sucker

Cypr

ni

ormes

Catostomidae

Catostomus fumeiven tris

owens sucker

Cypr

ni

ormes

Catostomidae

Catostomus plebeius

rio grande sucker

Cypr

ni

ormes

Catostomidae

Catostomus occidentalis

sacramento sucker

Cypr

ni

ormes

Catostomidae

Catostomus santaanae

santa ana sucker

Cypr

ni

ormes

Catostomidae

Catostomus insignis

sonora sucker

Cypr

ni

ormes

Catostomidae

Catostomus tahoensis

tahoe sucker

Cypr

ni

ormes

Catostomidae

Catostomus ardens

utah sucker

Cypr

ni

ormes

Catostomidae

Catostomus warnerensis

warner sucker

Cypr

ni

ormes

Catostomidae

Catostomus commersonii

white sucker

Cypr

ni

ormes

Catostomidae

Catostomus bernardini

yaqui sucker

Cypr

ni

ormes

Catostomidae

Chasmistes cujus

cui-ui

Cypr

ni

ormes

Catostomidae

Chasmistes liorus

june sucker

Cypr

ni

ormes

Catostomidae

Chasmistes brevirostris

shortnose sucker

Cypr

ni

ormes

Catostomidae

Chasmistes muriei

snake river sucker

Cypr

ni

ormes

Catostomidae

Cycleptus elongatus

blue sucker

Cypr

ni

ormes

Catostomidae

Cycleptus meridionalis

southeastern blue sucker

Cypr

ni

ormes

Catostomidae

Deltistes luxatus

lost river sucker

Cypr

ni

ormes

Catostomidae

Erimyzon oblongus

creek chubsucker

Cypr

ni

ormes

Catostomidae

Erimyzon sucetta

lake chubsucker

Cypr

ni

ormes

Catostomidae

Erimyzon tenuis

sharpfin chubsucker

Cypr

ni

ormes

Catostomidae

Hypentelium etowanum

alabama hog sucker

Cypr

ni

ormes

Catostomidae

Hypentelium nigricans

northern hog sucker

Cypr

ni

ormes

Catostomidae

Hypentelium roanokense

roanoke hog sucker

Cypr

ni

ormes

Catostomidae

Ictiobus cyprinellus

bigmouth buffalo

Cypr

ni

ormes

Catostomidae

Ictiobus niger

black buffalo

Cypr

ni

ormes

Catostomidae

Ictiobus bubalus

smallmouth buffalo

Cypr

ni

ormes

Catostomidae

Minytrema melanops

spotted sucker

Cypr

ni

ormes

Catostomidae

Moxostoma ariommum

bigeye jumprock

Cypr

ni

ormes

Catostomidae

Moxostoma duquesnei

black redhorse

Cypr

ni

ormes

Catostomidae

Moxostoma poecilurum

blacktail redhorse

Cypr

ni

ormes

Catostomidae

Moxostoma cervinum

blacktip jumprock

Cypr

ni

ormes

Catostomidae

Moxostoma erythrurum

golden redhorse

Cypr

ni

ormes

Catostomidae

Moxostoma congestum

gray redhorse

Cypr

ni

ormes

Catostomidae

Moxostoma lachneri

greater jumprock

Cypr

ni

ormes

Catostomidae

Moxostoma valenciennesi

greater redhorse

<
CO

<
Q

<
I

u_
u_

O

U~)
LU

<
u

u

i/l

Q
Z
<

o

o
u

Q
QC
<
Q

<
I—

i/l

Q
X
Q

CL
CL
<

D-4


-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME



Cypr

ni

ormes

Catostomidae

Moxostoma lacerum

harelip sucker



Cypr

ni

ormes

Catostomidae

Moxostoma austrinum

mexican redhorse



Cypr

ni

ormes

Catostomidae

Moxostoma collapsum

notchlip redhorse



Cypr

ni

ormes

Catostomidae

Moxostoma pisolabrum

pealip redhorse



Cypr

ni

ormes

Catostomidae

Moxostoma carinatum

river redhorse



Cypr

ni

ormes

Catostomidae

Moxostoma robustum

robust redhorse



Cypr

ni

ormes

Catostomidae

Moxostoma macrolepidotum

shorthead redhorse



Cypr

ni

ormes

Catostomidae

Moxostoma anisurum

silver redhorse



Cypr

ni

ormes

Catostomidae

Moxostoma breviceps

smallmouth redhorse



Cypr

ni

ormes

Catostomidae

Moxostoma rupiscartes

striped jumprock



Cypr

ni

ormes

Catostomidae

Moxostoma pappillosum

v-lip redhorse



Cypr

ni

ormes

Catostomidae

Moxostoma cf. poecilurum

apalachicola redhorse



Cypr

ni

ormes

Catostomidae

Moxostoma cf. lachneri

brassy jumprock



Cypr

ni

ormes

Catostomidae

Pantosteus lahontan

lahontan sucker



Cypr

ni

ormes

Catostomidae

Thoburnia atripinnis

blackfin sucker



Cypr

ni

ormes

Catostomidae

Thoburnia hamiltoni

rustyside sucker



Cypr

ni

ormes

Catostomidae

Thoburnia rhothoeca

torrent sucker

m

;z:

Cypr

ni

ormes

Catostomidae

Xyrauchen texanus

razorback sucker

<
CO

Cypr

ni

ormes

Cobitidae

Misgurnus anguillicaudatus

oriental weatherfish

I
o_

Cypr

ni

ormes

Cypr

nidae

Acrocheilus alutaceus

chiselmouth

<

Cypr

ni

ormes

Cypr

nidae

Agosia chrysogaster

longfin dace

Q

Cypr

ni

ormes

Cypr

nidae

Campostoma pauciradii

bluefin stoneroller

<
I

Cypr

ni

ormes

Cypr

nidae

Campostoma anomalum

central stoneroller


-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Cypr

niformes

Cypr

nidae

Cyprinella proserpina

proserpine shiner

Cypr

niformes

Cypr

nidae

Cyprinella lutrensis

red shiner

Cypr

niformes

Cypr

nidae

Cyprinella zanema

santee chub

Cypr

niformes

Cypr

nidae

Cyprinella analostana

satinfin shiner

Cypr

niformes

Cypr

nidae

Cyprinella spiloptera

spotfin shiner

Cypr

niformes

Cypr

nidae

Cyprinella whipplei

steelcolor shiner

Cypr

niformes

Cypr

nidae

Cyprinella gibbsi

tallapoosa shiner

Cypr

niformes

Cypr

nidae

Cyprinella labrosa

thicklip chub

Cypr

niformes

Cypr

nidae

Cyprinella trichroistia

tricolor shiner

Cypr

niformes

Cypr

nidae

Cyprinella nivea

whitefin shiner

Cypr

niformes

Cypr

nidae

Cyprinella galactura

whitetail shiner

Cypr

niformes

Cypr

nidae

Cyprinus carpio

common carp

Cypr

niformes

Cypr

nidae

Dionda diaboli

devils river minnow

Cypr

niformes

Cypr

nidae

Dionda nigrotaeniata

guadalupe roundnose
minnow

Cypriniformes

Cyprinidae

Dionda argentosa

manantial roundnose











minnow

Cypr

niformes

Cypr

nidae

Dionda serena

nueces roundnose minnow

Cypr

niformes

Cypr

nidae

Dionda episcopa

roundnose minnow

Cypr

niformes

Cypr

nidae

Eremichthys acros

desert dace

Cypr

niformes

Cypr

nidae

Erimonax monachus

spotfin chub

Cypr

niformes

Cypr

nidae

Erimystax insignis

blotched chub

Cypr

niformes

Cypr

nidae

Erimystax x-punctatus

gravel chub

Cypr

niformes

Cypr

nidae

Erimystax harryi

ozark chub

Cypr

niformes

Cypr

nidae

Erimystax cahni

slender chub

Cypr

niformes

Cypr

nidae

Erimystax dissimilis

streamline chub

Cypr

niformes

Cypr

nidae

Exoglossum maxillingua

cutlip minnow

Cypr

niformes

Cypr

nidae

Exoglossum laurae

tonguetied minnow

Cypr

niformes

Cypr

nidae

Gila alvordensis

alvord chub

Cypr

niformes

Cypr

nidae

Gila orcuttii

arroyo chub

Cypr

niformes

Cypr

nidae

Gila coerulea

blue chub

Cypr

niformes

Cypr

nidae

Gila elegans

bonytail

Cypr

niformes

Cypr

nidae

Gila boraxobius

borax lake chub

Cypr

niformes

Cypr

nidae

Gila nigrescens

chihuahua chub

Cypr

niformes

Cypr

nidae

Gila intermedia

gila chub

Cypr

niformes

Cypr

nidae

Gila nigra

headwater chub

Cypr

niformes

Cypr

nidae

Gila cypha

humpback chub

Cypr

niformes

Cypr

nidae

Gila pandora

rio grande chub

Cypr

niformes

Cypr

nidae

Gila robusta

roundtail chub

Cypr

niformes

Cypr

nidae

Gila ditaenia

sonora chub

Cypr

niformes

Cypr

nidae

Gila crassicauda

thicktail chub

Cypr

niformes

Cypr

nidae

Gila bicolor

tui chub

Cypr

niformes

Cypr

nidae

Gila atraria

utah chub

<
CO

<
Q

<
I

u_
u_

O

U~)
LU

<
u

u

i/l

Q
Z
<

o

o
u

Q
QC
<
Q

<
I—

i/l

Q
X
Q

CL
CL
<

D-6


-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME



Cypr

ni

ormes

Cypr

nidae

Gila seminuda

virgin chub



Cypr

ni

ormes

Cypr

nidae

Gila purpurea

yaqui chub



Cypr

ni

ormes

Cypr

nidae

Hemitremia flammea

flame chub



Cypr

ni

ormes

Cypr

nidae

Hesperoleucus symmetricus

California roach



Cypr

ni

ormes

Cypr

nidae

Hybognathus hankinsoni

brassy minnow



Cypr

ni

ormes

Cypr

nidae

Hybognathus hayi

cypress minnow



Cypr

ni

ormes

Cypr

nidae

Hybognathus regius

eastern silvery minnow



Cypr

ni

ormes

Cypr

nidae

Hybognathus nuchalis

mississippi silvery minnow



Cypr

ni

ormes

Cypr

nidae

Hybognathus placitus

plains minnow



Cypr

ni

ormes

Cypr

nidae

Hybognathus amarus

rio grande silvery minnow



Cypr

ni

ormes

Cypr

nidae

Hybognathus argyritis

western silvery minnow



Cypr

ni

ormes

Cypr

nidae

Hybopsis amblops

bigeye chub



Cypr

ni

ormes

Cypr

nidae

Hybopsis winchelli

clear chub



Cypr

ni

ormes

Cypr

nidae

Hybopsis hypsinotus

highback chub



Cypr

ni

ormes

Cypr

nidae

Hybopsis lineapunctata

lined chub



Cypr

ni

ormes

Cypr

nidae

Hybopsis amnis

pallid shiner



Cypr

ni

ormes

Cypr

nidae

Hybopsis rubrifrons

rosyface chub

m

;z:

Cypr

ni

ormes

Cypr

nidae

Hypophthalmichthys nobilis

bighead carp

<
CO

Cypr

ni

ormes

Cypr

nidae

Hypophthalmichthys molitrix

silver carp

I
o_

Cypr

ni

ormes

Cypr

nidae

lotichthys phlegethontis

least chub

<

Cypr

ni

ormes

Cypr

nidae

Lavinia exilicauda

hitch

Q
~Z.

Cypr

ni

ormes

Cypr

nidae

Lepidomeda vittata

little Colorado spinedace

<
I

Cypr

ni

ormes

Cypr

nidae

Lepidomeda altivelis

pahranagat spinedace

LO
U_

Cypr

ni

ormes

Cypr

nidae

Lepidomeda mollispinis

virgin spinedace

U_

O

Cypr

ni

ormes

Cypr

nidae

Lepidomeda albivallis

white river spinedace

m
LU

<

Cypr

ni

ormes

Cypr

nidae

Leuciscus idus

ide

Cypr

ni

ormes

Cypr

nidae

Luxilus zonistius

bandfin shiner

"Z.

y

Cypr

ni

ormes

Cypr

nidae

Luxilus zonatus

bleeding shiner

u_
H

Cypr

ni

ormes

Cypr

nidae

Luxilus cardinalis

cardinal shiner

Z

LU

Cypr

ni

ormes

Cypr

nidae

Luxilus cornutus

common shiner

U

CO

Cypr

ni

ormes

Cypr

nidae

Luxilus cerasinus

crescent shiner

O
Z

Cypr

ni

ormes

Cypr

nidae

Luxilus pilsbryi

duskystripe shiner

-Z.

Cypr

ni

ormes

Cypr

nidae

Luxilus chrysocephalus

striped shiner

o

Cypr

ni

ormes

Cypr

nidae

Luxilus coccogenis

warpaint shiner

o

Cypr

ni

ormes

Cypr

nidae

Luxilus albeolus

white shiner

u
o

Cypr

ni

ormes

Cypr

nidae

Lythrurus atrapiculus

blacktip shiner

cc
<

Cypr

ni

ormes

Cypr

nidae

Lythrurus roseipinnis

cherryfin shiner

o

Cypr

ni

ormes

Cypr

nidae

Lythrurus lirus

mountain shiner

<1.
1—

CO

Cypr

ni

ormes

Cypr

nidae

Lythrurus snelsoni

ouachita shiner

ci

Cypr

ni

ormes

Cypr

nidae

Lythrurus matutinus

pinewoods shiner

X

o

Cypr

ni

ormes

Cypr

nidae

Lythrurus bellus

pretty shiner

z

LU
n

Cypr

ni

ormes

Cypr

nidae

Lythrurus umbratilis

redfin shiner

o_
<

D-7


-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Cypr

ni

ormes

Cypr

nidae

Lythrurus fumeus

ribbon shiner

Cypr

ni

ormes

Cypr

nidae

Lythrurus ardens

rosefin shiner

Cypr

ni

ormes

Cypr

nidae

Lythrurus fasciolaris

scarlet shiner

Cypr

ni

ormes

Cypr

nidae

Lythrurus alegnotus

warrior shiner

Cypr

ni

ormes

Cypr

nidae

Macrhybopsis marconis

burrhead chub

Cypr

ni

ormes

Cypr

nidae

Macrhybopsis tetranema

peppered chub

Cypr

ni

ormes

Cypr

nidae

Macrhybopsis australis

prairie chub

Cypr

ni

ormes

Cypr

nidae

Macrhybopsis hyostoma

shoal chub

Cypr

ni

ormes

Cypr

nidae

Macrhybopsis meeki

sicklefin chub

Cypr

ni

ormes

Cypr

nidae

Macrhybopsis storeriana

silver chub

Cypr

ni

ormes

Cypr

nidae

Macrhybopsis aestivalis

speckled chub

Cypr

ni

ormes

Cypr

nidae

Macrhybopsis gelida

sturgeon chub

Cypr

ni

ormes

Cypr

nidae

Margariscus margarita

pearl dace

Cypr

ni

ormes

Cypr

nidae

Meda fulgida

spikedace

Cypr

ni

ormes

Cypr

nidae

Moapa coriacea

moapa dace

Cypr

ni

ormes

Cypr

nidae

Mylocheilus caurinus

peamouth

Cypr

ni

ormes

Cypr

nidae

Mylopharodon conocephalus

hardhead

Cypr

ni

ormes

Cypr

nidae

Mylopharyngodon piceus

black carp

Cypr

ni

ormes

Cypr

nidae

Nocomis platyrhynchus

bigmouth chub

Cypr

ni

ormes

Cypr

nidae

Nocomis leptocephalus

bluehead chub

Cypr

ni

ormes

Cypr

nidae

Nocomis raneyi

bull chub

Cypr

ni

ormes

Cypr

nidae

Nocomis biguttatus

hornyhead chub

Cypr

ni

ormes

Cypr

nidae

Nocomis asper

redspot chub

Cypr

ni

ormes

Cypr

nidae

Nocomis effusus

redtail chub

Cypr

ni

ormes

Cypr

nidae

Nocomis micropogon

river chub

Cypr

ni

ormes

Cypr

nidae

Notemigonus crysoleucas

golden shiner

Cypr

ni

ormes

Cypr

nidae

Notropis girardi

arkansas river shiner

Cypr

ni

ormes

Cypr

nidae

Notropis rupestris

bedrock shiner

Cypr

ni

ormes

Cypr

nidae

Notropis boops

bigeye shiner

Cypr

ni

ormes

Cypr

nidae

Notropis dorsal is

bigmouth shiner

Cypr

ni

ormes

Cypr

nidae

Notropis heterodon

blackchin shiner

Cypr

ni

ormes

Cypr

nidae

Notropis melanostomus

blackmouth shiner

Cypr

ni

ormes

Cypr

nidae

Notropis heterolepis

blacknose shiner

Cypr

ni

ormes

Cypr

nidae

Notropis atrocaudalis

blackspot shiner

Cypr

ni

ormes

Cypr

nidae

Notropis simus

bluntnose shiner

Cypr

ni

ormes

Cypr

nidae

Notropis bifrenatus

bridle shiner

Cypr

ni

ormes

Cypr

nidae

Notropis asperifrons

burrhead shiner

Cypr

ni

ormes

Cypr

nidae

Notropis cahabae

cahaba shiner

Cypr

ni

ormes

Cypr

nidae

Notropis mekistocholas

cape fear shiner

Cypr

ni

ormes

Cypr

nidae

Notropis percobromus

carmine shiner

Cypr

ni

ormes

Cypr

nidae

Notropis wickliffi

channel shiner

Cypr

ni

ormes

Cypr

nidae

Notropis chihuahua

chihuahua shiner

<
CO

<
Q

<
I

u_
u_

O

U~)
LU

<
u

u

i/l

Q
Z
<

o

o
u

Q
QC
<
Q

<
I—

i/l

Q
X
Q

CL
CL
<

D-8


-------
National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME



Cypr

ni

ormes

Cypr

nidae

Notropis potteri

chub shiner



Cypr

ni

ormes

Cypr

nidae

Notropis petersoni

coastal shiner



Cypr

ni

ormes

Cypr

nidae

Notropis amoenus

comely shiner



Cypr

ni

ormes

Cypr

nidae

Notropis xaenocephalus

coosa shiner



Cypr

ni

ormes

Cypr

nidae

Notropis cummingsae

dusky shiner



Cypr

ni

ormes

Cypr

nidae

Notropis atherinoides

emerald shiner



Cypr

ni

ormes

Cypr

nidae

Notropis edwardraneyi

fluvial shiner



Cypr

ni

ormes

Cypr

nidae

Notropis buchanani

ghost shiner



Cypr

ni

ormes

Cypr

nidae

Notropis chlorocephalus

greenhead shiner



Cypr

ni

ormes

Cypr

nidae

Notropis altipinnis

highfin shiner



Cypr

ni

ormes

Cypr

nidae

Notropis micropteryx

highland shiner



Cypr

ni

ormes

Cypr

nidae

Notropis hypsilepis

highscale shiner



Cypr

ni

ormes

Cypr

nidae

Notropis chalybaeus

ironcolor shiner



Cypr

ni

ormes

Cypr

nidae

Notropis ortenburgeri

kiamichi shiner



Cypr

ni

ormes

Cypr

nidae

Notropis longirostris

longnose shiner



Cypr

ni

ormes

Cypr

nidae

Notropis volucellus

mimic shiner



Cypr

ni

ormes

Cypr

nidae

Notropis spectrunculus

mirror shiner

i/i

z

Cypr

ni

ormes

Cypr

nidae

Notropis scabriceps

new river shiner

<
CO

Cypr

ni

ormes

Cypr

nidae

Notropis ammophilus

orangefin shiner

I

CL

Cypr

ni

ormes

Cypr

nidae

Notropis nubilus

ozark minnow

<

Cypr

ni

ormes

Cypr

nidae

Notropis ozarcanus

ozark shiner

Q

z

Cypr

ni

ormes

Cypr

nidae

Notropis albizonatus

palezone shiner

<
I

Cypr

ni

ormes

Cypr

nidae

Notropis perpallidus

peppered shiner

LI-

Cypr

ni

ormes

Cypr

nidae

Notropis orca

phantom shiner

LI.

O

Cypr

ni

ormes

Cypr

nidae

Notropis ariommus

popeye shiner

l/l
LU

Cypr

ni

ormes

Cypr

nidae

Notropis a nog en us

pugnose shiner

<

Cypr

ni

ormes

Cypr

nidae

Notropis chrosomus

rainbow shiner

Z

U

Cypr

ni

ormes

Cypr

nidae

Notropis bairdi

red river shiner

Ll_

1—

Cypr

ni

ormes

Cypr

nidae

Notropis harperi

redeye chub

Z

LU

Cypr

ni

ormes

Cypr

nidae

Notropis chiliticus

redlip shiner

u

l/l

Cypr

ni

ormes

Cypr

nidae

Notropis jemezanus

rio grande shiner

O
Z

Cypr

ni

ormes

Cypr

nidae

Notropis blennius

river shiner

Z

Cypr

ni

ormes

Cypr

nidae

Notropis suttkusi

rocky shiner

o

Cypr

ni

ormes

Cypr

nidae

Notropis rubellus

rosyface shiner

o

Cypr

ni

ormes

Cypr

nidae

Notropis baileyi

rough shiner

u

Q

Cypr

ni

ormes

Cypr

nidae

Notropis semperasper

roughhead shiner

QC
<

Cypr

ni

ormes

Cypr

nidae

Notropis sabinae

sabine shiner

o
z

Cypr

ni

ormes

Cypr

nidae

Notropis rubricroceus

saffron shiner


-------
National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Cypr

ni

ormes

Cypr

nidae

Notropis shumardi

silverband shiner

Cypr

ni

ormes

Cypr

nidae

Notropis buccatus

silverjaw minnow

Cypr

ni

ormes

Cypr

nidae

Notropis can did us

silverside shiner

Cypr

ni

ormes

Cypr

nidae

Notropis stilbius

silverstripe shiner

Cypr

ni

ormes

Cypr

nidae

Notropis uranoscopus

skygazer shiner

Cypr

ni

ormes

Cypr

nidae

Notropis buccula

smalleye shiner

Cypr

ni

ormes

Cypr

nidae

Notropis hudsonius

spottail shiner

Cypr

ni

ormes

Cypr

nidae

Notropis procne

swallowtail shiner

Cypr

ni

ormes

Cypr

nidae

Notropis maculatus

taillight shiner

Cypr

ni

ormes

Cypr

nidae

Notropis braytoni

tamaulipas shiner

Cypr

ni

ormes

Cypr

nidae

Notropis telescopus

telescope shiner

Cypr

ni

ormes

Cypr

nidae

Notropis leuciodus

tennessee shiner

Cypr

ni

ormes

Cypr

nidae

Notropis amabilis

texas shiner

Cypr

ni

ormes

Cypr

nidae

Notropis topeka

topeka shiner

Cypr

ni

ormes

Cypr

nidae

Notropis greenei

wedgespot shiner

Cypr

ni

ormes

Cypr

nidae

Notropis texanus

weed shiner

Cypr

ni

ormes

Cypr

nidae

Notropis alborus

whitemouth shiner

Cypr

ni

ormes

Cypr

nidae

Notropis rafinesquei

yazoo shiner

Cypr

ni

ormes

Cypr

nidae

Notropis lutipinnis

yellowfin shiner

Cypr

ni

ormes

Cypr

nidae

Opsopoeodus emiliae

pugnose minnow

Cypr

ni

ormes

Cypr

nidae

Oregonichthys crameri

oregon chub

Cypr

ni

ormes

Cypr

nidae

Oregonichthys kalawatseti

umpqua chub

Cypr

ni

ormes

Cypr

nidae

Orthodon microlepidotus

sacramento blackfish

Cypr

ni

ormes

Cypr

nidae

Phenacobius crassilabrum

fatlips minnow

Cypr

ni

ormes

Cypr

nidae

Phenacobius teretulus

kanawha minnow

Cypr

ni

ormes

Cypr

nidae

Phenacobius catostomus

riffle minnow

Cypr

ni

ormes

Cypr

nidae

Phenacobius uranops

stargazing minnow

Cypr

ni

ormes

Cypr

nidae

Phenacobius mirabilis

suckermouth minnow

Cypr

ni

ormes

Cypr

nidae

Phoxinus cumberlandensis

blackside dace

Cypr

ni

ormes

Cypr

nidae

Phoxinus neogaeus

finescale dace

Cypr

ni

ormes

Cypr

nidae

Phoxinus saylori

laurel dace

Cypr

ni

ormes

Cypr

nidae

Phoxinus oreas

mountain redbelly dace

Cypr

ni

ormes

Cypr

nidae

Phoxinus eos

northern redbelly dace

Cypr

ni

ormes

Cypr

nidae

Phoxinus erythrogaster

southern redbelly dace

Cypr

ni

ormes

Cypr

nidae

Phoxinus tennesseensis

tennessee dace

Cypr

ni

ormes

Cypr

nidae

Pimephales notatus

bluntnose minnow

Cypr

ni

ormes

Cypr

nidae

Pimephales vigilax

bullhead minnow

Cypr

ni

ormes

Cypr

nidae

Pimephales promelas

fathead minnow

Cypr

ni

ormes

Cypr

nidae

Pimephales tenellus

slim minnow

Cypr

ni

ormes

Cypr

nidae

Plagopterus argentissimus

woundfin

Cypr

ni

ormes

Cypr

nidae

Platygobio gracilis

flathead chub

Cypr

ni

ormes

Cypr

nidae

Pogonichthys ciscoides

clear lake splittail


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER	FAMILY	SCIENTIFIC NAME	COMMON NAME

Cypr

ni

ormes

Cypr

nidae

Pogonichthys macrolepidotus

splittail

Cypr

ni

ormes

Cypr

nidae

Pteronotropis grandipinnis

apalachee shiner

Cypr

ni

ormes

Cypr

nidae

Pteronotropis hubbsi

bluehead shiner

Cypr

ni

ormes

Cypr

nidae

Pteronotropis welaka

bluenose shiner

Cypr

ni

ormes

Cypr

nidae

Pteronotropis euryzonus

broadstripe shiner

Cypr

ni

ormes

Cypr

nidae

Pteronotropis signipinnis

flagfin shiner

Cypr

ni

ormes

Cypr

nidae

Pteronotropis merlini

orangetail shiner

Cypr

ni

ormes

Cypr

nidae

Pteronotropis hypselopterus

sailfin shiner

Cypr

ni

ormes

Cypr

nidae

Ptychocheilus lucius

Colorado pikeminnow

Cypr

ni

ormes

Cypr

nidae

Ptychocheilus oregonensis

northern pikeminnow

Cypr

ni

ormes

Cypr

nidae

Ptychocheilus grandis

sacramento pikeminnow

Cypr

ni

ormes

Cypr

nidae

Ptychocheilus umpquae

umpqua pikeminnow

Cypr

ni

ormes

Cypr

nidae

Relictus solitarius

relict dace

Cypr

ni

ormes

Cypr

nidae

Rhinichthys atratulus

eastern blacknose dace

Cypr

ni

ormes

Cypr

nidae

Rhinichthys deaconi

las vegas dace

Cypr

ni

ormes

Cypr

nidae

Rhinich thys fa lea tus

leopard dace

Cypr

ni

ormes

Cypr

nidae

Rhinichthys cobitis

loach minnow

Cypr

ni

ormes

Cypr

nidae

Rhinichthys cataractae

longnose dace

Cypr

ni

ormes

Cypr

nidae

Rhinichthys osculus

speckled dace

Cypr

ni

ormes

Cypr

nidae

Rhinichthys umatilla

umatilla dace

Cypr

ni

ormes

Cypr

nidae

Rhinichthys evermanni

umpqua dace

Cypr

ni

ormes

Cypr

nidae

Rhinichthys obtusus

western blacknose dace

Cypr

ni

ormes

Cypr

nidae

Rhodeus sericeus

bitterling

Cypr

ni

ormes

Cypr

nidae

Richardsonius egregius

lahontan redside

Cypr

ni

ormes

Cypr

nidae

Richardsonius balteatus

redside shiner

Cypr

ni

ormes

Cypr

nidae

Richardsonius X Rhinichthys

redside shiner x speckled











balteatus x osculus

dace

Cypr

ni

ormes

Cypr

nidae

Scardinius erythrophthalmus

rudd

Cypr

ni

ormes

Cypr

nidae

Semotilus atromaculatus

creek chub

Cypr

ni

ormes

Cypr

nidae

Semotilus thoreauianus

dixie chub

Cypr

ni

ormes

Cypr

nidae

Semotilus corporalis

fallfish

Cypr

ni

ormes

Cypr

nidae

Semotilus lumbee

sandhills chub

Cypr

ni

ormes

Cypr

nidae

Snyderichthys copei

leatherside chub

Cypr

ni

ormes

Cypr

nidae

Tinea tinea

tench

Cypr

ni

ormes

Cypr

nidae

Cyprinella cf. zanema

thinlip chub

Cypr

ni

ormes

Cypr

nidae

Hypophthalmichthys molitrix

silver carp

Cypr

ni

ormes

Catostomidae

Catostomus cf. latipinnis

little Colorado river sucker

Cypr

ni

ormes

Catostomidae

Moxostoma robustum

smallfin redhorse

Cypr

ni

ormes

Cyprinidae

Macrhybopsis cf aestivalis

coosa chub

Cypr

ni

ormes

Cyprinidae

Semotilus X Luxilus

creek chub x striped shiner

atromaculatus x
chrysocephalus


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER



FAMILY

SCIENTIFIC NAME

COMMON NAME

Cypr

niformes

Cyprinidae

Cyprinus carpio

mirror carp

Cypr

niformes

Cyprinidae

Hypophthalmichthys nobilis

bighead carp

Cypr

niformes

Cyprinidae

Notropis amplamala

longjaw minnow

Cypr

niformes

Cyprinidae

Notropis cf. spectrunculus

sawfin shiner

Cypr

niformes

Cyprinidae

Pteronotropis stonei

lowland shiner

Cypr

nodont

formes

Aplocheilidae

Rivulus hartii

giant rivulus

Cypr

nodont

formes

Aplocheilidae

Rivulus marmoratus

mangrove rivulus

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon nevadensis

amargosa pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon elegans

comanche springs pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon eximius

conchos pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon macularius

desert pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon diabolis

devils hole pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon bovinus

leon springs pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon radiosus

owens pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon pecosensis

pecos pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon rubrofluviatilis

red river pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon sal in us

salt creek pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon arcuatus

santa cruz pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon variegatus

sheepshead minnow

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon eremus

sonoyta pupfish

Cypr

nodont

formes

Cypr

nodontidae

Cyprinodon tularosa

white sands pupfish

Cypr

nodont

formes

Cypr

nodontidae

Jordanella floridae

flagfish

Cypr

nodont

formes

Fundulidae

Fundulus diaphanus

banded killifish

Cypr

nodont

formes

Fundulidae

Fundulus cingulatus

banded topminnow

Cypr

nodont

formes

Fundulidae

Fundulus julisia

barrens topminnow

Cypr

nodont

formes

Fundulidae

Fundulus pulvereus

bayou killifish

Cypr

nodont

formes

Fundulidae

Fundulus nottii

bayou topminnow

Cypr

nodont

formes

Fundulidae

Fundulus olivaceus

blackspotted topminnow

Cypr

nodont

formes

Fundulidae

Fundulus notatus

blackstripe topminnow

Cypr

nodont

formes

Fundulidae

Fundulus euryzonus

broadstripe topminnow

Cypr

nodont

formes

Fundulidae

Fundulus chrysotus

golden topminnow

Cypr

nodont

formes

Fundulidae

Fundulus parvipinnis

guadalupe cardinalfish

Cypr

nodont

formes

Fundulidae

Fundulus grandis

gulf killifish

Cypr

nodont

formes

Fundulidae

Fundulus lineolatus

lined topminnow

Cypr

nodont

formes

Fundulidae

Fundulus confluentus

marsh killifish

Cypr

nodont

formes

Fundulidae

Fundulus heteroclitus

mummichog

Cypr

nodont

formes

Fundulidae

Fundulus kansae

northern plains killifish

Cypr

nodont

formes

Fundulidae

Fundulus catenatus

northern studfish

Cypr

nodont

formes

Fundulidae

Fundulus zebrinus

plains killifish

Cypr

nodont

formes

Fundulidae

Fundulus sciadieus

plains topminnow

Cypr

nodont

formes

Fundulidae

Fundulus rubrifrons

redface topminnow

Cypr

nodont

formes

Fundulidae

Fundulus escambiae

russetfin topminnow


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER



FAMILY

SCIENTIFIC NAME

COMMON NAME

Cypr

nodont

formes

Fundulidae

Fundulus jenkinsi

saltmarsh topminnow

Cypr

nodont

formes

Fundulidae

Fundulus seminolis

seminole killifish

Cypr

nodont

formes

Fundulidae

Fundulus stellifer

southern studfish

Cypr

nodont

formes

Fundulidae

Fundulus rathbuni

speckled killifish

Cypr

nodont

formes

Fundulidae

Fundulus luciae

spotfin killifish

Cypr

nodont

formes

Fundulidae

Fundulus dlspar

starhead topminnow

Cypr

nodont

formes

Fundulidae

Fundulus blfax

stippled studfish

Cypr

nodont

formes

Fundulidae

Fundulus waccamensls

waccamaw killifish

Cypr

nodont

formes

Fundulidae

Fundulus blalrae

western starhead topminnow

Cypr

nodont

formes

Fundulidae

Fundulus albolineatus

whiteline topminnow

Cypr

nodont

formes

Fundulidae

Leptolucanla ommata

pygmy killifish

Cypr

nodont

formes

Fundulidae

Lucanla goodei

bluefin killifish

Cypr

nodont

formes

Fundulidae

Lucanla parva

rainwater killifish

Cypr

nodont

formes

Goodeidae

Crenlchthys nevadae

railroad valley springfish

Cypr

nodont

formes

Goodeidae

Crenlchthys baileyl

white river springfish

Cypr

nodont

formes

Goodeidae

Empetrichthys merriami

ash meadows poolfish

Cypr

nodont

formes

Goodeidae

Empetrichthys latos

pahrump poolfish

Cypr

nodont

formes

Poecil

idae

Belonesox bellzanus

pike killifish

Cypr

nodont

formes

Poecil

idae

Gambusla amistadensls

amistad gambusia

Cypr

nodont

formes

Poecil

idae

Gam bus! a gaigei

big bend gambusia

Cypr

nodont

formes

Poecil

idae

Gambusla senilis

blotched gambusia

Cypr

nodont

formes

Poecil

idae

Gambusia heterochir

clear creek gambusia

Cypr

nodont

formes

Poecil

idae

Gambusia holbrooki

eastern mosquitofish

Cypr

nodont

formes

Poecil

idae

Gambusia geiseri

largespring gambusia

Cypr

nodont

formes

Poecil

idae

Gambusia rhizophorae

mangrove gambusia

Cypr

nodont

formes

Poecil

idae

Gambusia nobilis

pecos gambusia

Cypr

nodont

formes

Poecil

idae

Gambusia georgei

san marcos gambusia

Cypr

nodont

formes

Poecil

idae

Gambusia speciosa

tex-mex gambusia

Cypr

nodont

formes

Poecil

idae

Gambusia affinis

western mosquitofish

Cypr

nodont

formes

Poecil

idae

Heterandria formosa

least killifish

Cypr

nodont

formes

Poecil

idae

Poecil ia formosa

amazon molly

Cypr

nodont

formes

Poecil

idae

Poecilia reticulata

guppy

Cypr

nodont

formes

Poecil

idae

Poecilia sphenops

mexican molly

Cypr

nodont

formes

Poecil

idae

Poecilia latipinna

sailfin molly

Cypr

nodont

formes

Poecil

idae

Poecilia mexicana

shortfin molly

Cypr

nodont

formes

Poecil

idae

Poeciliopsis occidentalis

gila topminnow

Cypr

nodont

formes

Poecil

idae

Poeciliopsis gracilis

porthole livebearer

Cypr

nodont

formes

Poecil

idae

Xiphophorus hellerii

green swordtail

Cypr

nodont

formes

Poecil

idae

Xiphophorus maculatus

southern platyfish

Cypr

nodont

formes

Poecil

idae

Xiphophorus variatus

variable platyfish

Elopiformes



Elopidae

Elops saurus

ladyfish

Elopiformes



Elopidae

Elops affinis

machete


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Elopiformes

Megalopidae

Megalops atlanticus

tarpon

Esociformes

Esocidae

Esox niger

chain pickerel

Esociformes

Esocidae

Esox masquinongy

muskellunge

Esociformes

Esocidae

Esox lucius

northern pike

Esociformes

Esocidae

Esox americanus

redfin pickerel

Esociformes

Esocidae

Esox americanus vermiculatus

grass pickerel

Esociformes

Esocidae

Esox lucius x masquinongy

tiger muskellunge

Esociformes

Umbridae

Dallia pectoralis

alaska blackfish

Esociformes

Umbridae

Novum bra hubbsi

Olympic mudminnow

Esociformes

Umbridae

Umbra limi

central mudminnow

Esociformes

Umbridae

Umbra pygmaea

eastern mudminnow

Gadiformes

Gadidae

Lota lota

burbot

Gadiformes

Gadidae

Microgadus tomcod

atlantic tomcod

Gasterosteiformes

Gasterosteidae

Apeltes quadracus

fourspine stickleback

Gasterosteiformes

Gasterosteidae

Culaea inconstans

brook stickleback

Gasterosteiformes

Gasterosteidae

Gasterosteus aculeatus

espinocho

Gasterosteiformes

Gasterosteidae

Pungitius pungitius

ninespine stickleback

Gasterosteiformes

Syngnathidae

Microphis brachyurus

opossum pipefish

Gasterosteiformes

Syngnathidae

Syngnathus scovelli

gulf pipefish

Gasterosteiformes

Gasterosteidae

Gasterosteus aculeatus

threespine stickleback

Hiodontiformes

Hiodontidae

Hiodon alosoides

goldeye

Hiodontiformes

Hiodontidae

Hiodon tergisus

mooneye

Lepisosteiformes

Lepisosteidae

Atractosteus spatula

alligator gar

Lepisosteiformes

Lepisosteidae

Lepisosteus platyrhincus

florida gar

Lepisosteiformes

Lepisosteidae

Lepisosteus osseus

longnose gar

Lepisosteiformes

Lepisosteidae

Lepisosteus platostomus

shortnose gar

Lepisosteiformes

Lepisosteidae

Lepisosteus oculatus

spotted gar

Mugiliformes

Mugilidae

Agonostomus monticola

mountain mullet

Mugiliformes

Mugilidae

Mugil cephalus

striped mullet

Mugiliformes

Mugilidae

Mugil cur em a

white mullet

Myliobatiformes

Dasyatidae

Dasyatis sabina

atlantic stingray

Osteoglossiformes

Notopteridae

Chitala ornata

clown knifefish

Perciformes

Belontiidae

Trichopsis vittata

croaking gourami

Perciformes

Centrarchidae

Acantharchus pomotis

mud sunfish

Perciformes

Centrarchidae

Ambloplites constellatus

ozark bass

Perciformes

Centrarchidae

Ambloplites cavifrons

roanoke bass

Perciformes

Centrarchidae

Ambloplites rupestris

rock bass

Perciformes

Centrarchidae

Ambloplites ariommus

shadow bass

Perciformes

Centrarchidae

Archoplites interruptus

sacramento perch

Perciformes

Centrarchidae

Centrarchus macropterus

flier

Perciformes

Centrarchidae

Enneacanthus obesus

banded sunfish

Perciformes

Centrarchidae

Enneacanthus chaetodon

blackbanded sunfish


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Perciformes

Centrarchidae

Enneacanthus gloriosus

bluespotted sunfish

Perciformes

Centrarchidae

Lepomis symmetricus

bantam sunfish

Perciformes

Centrarchidae

Lepomis macrochirus

bluegill

Perciformes

Centrarchidae

Lepomis marginatus

dollar sunfish

Perciformes

Centrarchidae

Lepomis cyanellus

green sunfish

Perciformes

Centrarchidae

Lepomis megalotis

longear sunfish

Perciformes

Centrarchidae

Lepomis humilis

orangespotted sunfish

Perciformes

Centrarchidae

Lepomis gibbosus

pumpkinseed

Perciformes

Centrarchidae

Lepomis auritus

redbreast sunfish

Perciformes

Centrarchidae

Lepomis microlophus

redear sunfish

Perciformes

Centrarchidae

Lepomis miniatus

redspotted sunfish

Perciformes

Centrarchidae

Lepomis punctatus

spotted sunfish

Perciformes

Centrarchidae

Lepomis gulosus

warmouth

Perciformes

Centrarchidae

Micropterus treculii

guadalupe bass

Perciformes

Centrarchidae

Micropterus salmoides

largemouth bass

Perciformes

Centrarchidae

Micropterus coosae

redeye bass

Perciformes

Centrarchidae

Micropterus cataractae

shoal bass

Perciformes

Centrarchidae

Micropterus dolomieu

smallmouth bass

Perciformes

Centrarchidae

Micropterus punctulatus

spotted bass

Perciformes

Centrarchidae

Micropterus notius

suwannee bass

Perciformes

Centrarchidae

Pomoxis nigromaculatus

black crappie

Perciformes

Centrarchidae

Pomoxis annularis

white crappie

Perciformes

Centropomidae

Centropomus undecimalis

common snook

Perciformes

Centropomidae

Centropomus parallel us

smallscale fat snook

Perciformes

Centropomidae

Centropomus ensiferus

swordspine snook

Perciformes

Centropomidae

Centropomus pectinatus

tarpon snook

Perciformes

Channidae

Channa marulius

bullseye snakehead

Perciformes

Channidae

Channa argus

snakehead

Perciformes

Cichl

dae

Astronotus ocellatus

oscar

Perciformes

Cichl

dae

Cichla ocellaris

butterfly peacock bass

Perciformes

Cichl

dae

Cichlasoma bimaculatum

black acara

Perciformes

Cichl

dae

Cichlasoma nigrofasciatum

convict cichlid

Perciformes

Cichl

dae

Cichlasoma meeki

firemouth cichlid

Perciformes

Cichl

dae

Cichlasoma octofasciatum

jack dempsey

Perciformes

Cichl

dae

Cichlasoma managuense

jaguar guapote

Perciformes

Cichl

dae

Cichlasoma urophthalmus

mayan cichlid

Perciformes

Cichl

dae

Cichlasoma citrinellum

midas cichlid

Perciformes

Cichl

dae

Cichlasoma cyanoguttatum

rio grande cichlid

Perciformes

Cichl

dae

Cichlasoma salvini

yellowbelly cichlid

Perciformes

Cichl

dae

Geophagus surinamensis

redstriped eartheater

Perciformes

Cichl

dae

Hemichromis letourneuxi

african j ewe If is h

Perciformes

Cichl

dae

Heros severus

banded cichlid


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Perciformes

Cichlidae

Oreochromis aureus

blue tilapia

Perciformes

Cichlidae

Oreochromis mossambicus

mozambique tilapia

Perciformes

Cichlidae

Oreochromis niloticus

nile tilapia

Perciformes

Cichlidae

Oreochromis urolepis

wami tilapia

Perciformes

Cichlidae

Sarotherodon melanotheron

blackchin tilapia

Perciformes

Cichlidae

Tilapia zillii

red belly tilapia

Perciformes

Cichlidae

Til a pi a mariae

spotted tilapia

Perciformes

Elassomatidae

Elassoma zonatum

banded pygmy sunfish

Perciformes

Elassomatidae

Elassoma okatie

bluebarred pygmy sunfish

Perciformes

Elassomatidae

Elassoma boehlkei

Carolina pygmy sunfish

Perciformes

Elassomatidae

Elassoma evergladei

everglades pygmy sunfish

Perciformes

Elassomatidae

Elassoma okefenokee

okefenokee pygmy sunfish

Perciformes

Elassomatidae

Elassoma alabamae

spring pygmy sunfish

Perciformes

Eleotridae

Dormitator maculatus

fat sleeper

Perciformes

Eleotridae

Eleotris amblyopsis

largescaled spinycheek

Perciformes

Eleotridae

Eleotris perniger

smallscaled spinycheek

Perciformes

Eleotridae

Eleotris picta

spotted sleeper

Perciformes

Eleotridae

Gobiomorus dormitor

bigmouth sleeper

Perciformes

Eleotridae

Guavina guavina

guavina

Perciformes

Eleotridae

Eleotris amblyopsis

largescaled spinycheek









sleeper

Perciformes

Embiotocidae

Cymatogaster aggregata

shiner perch

Perciformes

Embiotocidae

Hysterocarpus traskii

tule perch

Perciformes

Gerreidae

Diapterus auratus

irish pompano

Perciformes

Gerreidae

Eucinostomus harengulus

tidewater mojarra

Perciformes

Gerreidae

Eugerres plumieri

striped mojarra

Perciformes

Gerreidae

Gerres cinereus

yellowfin mojarra

Perciformes

Gob

idae

Acanthogobius flavimanus

yellowfin goby

Perciformes

Gob

idae

Awaous banana

river goby

Perciformes

Gob

idae

Clevelandia ios

arrow goby

Perciformes

Gob

idae

Ctenogobius fasciatus

blotchcheek goby

Perciformes

Gob

idae

Ctenogobius boleosoma

darter goby

Perciformes

Gob

idae

Ctenogobius shufeldti

freshwater goby

Perciformes

Gob

idae

Ctenogobius claytonii

mexican goby

Perciformes

Gob

idae

Ctenogobius pseudofasciatus

slashcheek goby

Perciformes

Gob

idae

Eucyclogobius newberryi

tidewater goby

Perciformes

Gob

idae

Gillichthys mirabilis

longjaw mudsucker

Perciformes

Gob

idae

Gobioides broussonetii

violet goby

Perciformes

Gob

idae

Gobiosoma bosc

naked goby

Perciformes

Gob

idae

Lophogobius cyprinoides

crested goby

Perciformes

Gob

idae

Microgobius gulosus

clown goby

Perciformes

Gob

idae

Neogobius melanostomus

round goby


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Perciformes

Gobiidae

Proterorhinus marmoratus

tubenose goby

Perciformes

Gobiidae

Tridentiger bifasciatus

shimofuri goby

Perciformes

Gobiidae

Triden tiger barbatus

shokihaze goby

Perciformes

Haemulidae

Orthopristis chrysoptera

pigfish

Perciformes

Lutjanidae

Lutjanus griseus

gray snapper

Perciformes

Moronidae

Morone saxatilis

striped bass

Perciformes

Moronidae

Morone chrysops

white bass

Perciformes

Moronidae

Morone americana

white perch

Perciformes

Moronidae

Morone mississippiensis

yellow bass

Perciformes

Moronidae

Morone na

wiper

Perciformes

Percidae

Ammocrypta pellucida

eastern sand darter

Perciformes

Percidae

Ammocrypta bifascia

florida sand darter

Perciformes

Percidae

Ammocrypta beanii

naked sand darter

Perciformes

Percidae

Ammocrypta vivax

scaly sand darter

Perciformes

Percidae

Ammocrypta meridiana

southern sand darter

Perciformes

Percidae

Ammocrypta clara

western sand darter

Perciformes

Percidae

Crystal/aria asprella

crystal darter

Perciformes

Percidae

Etheostoma ramseyi

alabama darter

Perciformes

Percidae

Etheostoma cragini

arkansas darter

Perciformes

Percidae

Etheostoma euzonum

arkansas saddled darter

Perciformes

Percidae

Etheostoma sagitta

arrow darter

Perciformes

Percidae

Etheostoma cinereum

ashy darter

Perciformes

Percidae

Etheostoma zonifer

backwater darter

Perciformes

Percidae

Etheostoma zonale

banded darter

Perciformes

Percidae

Etheostoma zonistium

bandfin darter

Perciformes

Percidae

Etheostoma obeyense

barcheek darter

Perciformes

Percidae

Etheostoma forbesi

barrens darter

Perciformes

Percidae

Etheostoma rubrum

bayou darter

Perciformes

Percidae

Etheostoma nigripinne

blackfin darter

Perciformes

Percidae

Etheostoma duryi

blackside snubnose darter

Perciformes

Percidae

Etheostoma blennius

blenny darter

Perciformes

Percidae

Etheostoma sanguifluum

bloodfin darter

Perciformes

Percidae

Etheostoma camurum

bluebreast darter

Perciformes

Percidae

Etheostoma jessiae

blueside darter

Perciformes

Percidae

Etheostoma chlorosoma

bluntnose darter

Perciformes

Percidae

Etheostoma wapiti

boulder darter

Perciformes

Percidae

Etheostoma lynceum

brighteye darter

Perciformes

Percidae

Etheostoma burri

brook darter

Perciformes

Percidae

Etheostoma edwini

brown darter

Perciformes

Percidae

Etheostoma bison

buffalo darter

Perciformes

Percidae

Etheostoma osburni

candy darter

Perciformes

Percidae

Etheostoma collis

Carolina darter


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Perciformes

Percidae

Etheostoma scotti

Cherokee darter

Perciformes

Percidae

Etheostoma etnieri

cherry darter

Perciformes

Percidae

Etheostoma cervus

chickasaw darter

Perciformes

Percidae

Etheostoma davisoni

choctawhatchee darter

Perciformes

Percidae

Etheostoma hopkinsi

Christmas darter

Perciformes

Percidae

Etheostoma colorosum

coastal darter

Perciformes

Percidae

Etheostoma ditrema

coldwater darter

Perciformes

Percidae

Etheostoma coosae

coosa darter

Perciformes

Percidae

Etheostoma aquali

coppercheek darter

Perciformes

Percidae

Etheostoma basilare

corrugated darter

Perciformes

Percidae

Etheostoma collettei

Creole darter

Perciformes

Percidae

Etheostoma corona

crown darter

Perciformes

Percidae

Etheostoma susanae

Cumberland darter

Perciformes

Percidae

Etheostoma uniporum

current darter

Perciformes

Percidae

Etheostoma proeliare

cypress darter

Perciformes

Percidae

Etheostoma percnurum

duskytail darter

Perciformes

Percidae

Etheostoma pseudovulatum

egg-mimic darter

Perciformes

Percidae

Etheostoma baileyi

emerald darter

Perciformes

Percidae

Etheostoma etowahae

etowah darter

Perciformes

Percidae

Etheostoma flabellare

fantail darter

Perciformes

Percidae

Etheostoma pyrrhogaster

firebelly darter

Perciformes

Percidae

Etheostoma fonticola

fountain darter

Perciformes

Percidae

Etheostoma crossopterum

fringed darter

Perciformes

Percidae

Etheostoma vitreum

glassy darter

Perciformes

Percidae

Etheostoma denoncourti

golden darter

Perciformes

Percidae

Etheostoma parvipinne

goldstripe darter

Perciformes

Percidae

Etheostoma jordani

greenbreast darter

Perciformes

Percidae

Etheostoma chlorobranchium

greenfin darter

Perciformes

Percidae

Etheostoma blennioides

greenside darter

Perciformes

Percidae

Etheostoma lepidum

greenthroat darter

Perciformes

Percidae

Etheostoma oophylax

guardian darter

Perciformes

Percidae

Etheostoma swaini

gulf darter

Perciformes

Percidae

Etheostoma histrio

harlequin darter

Perciformes

Percidae

Etheostoma lawrencei

headwater darter

Perciformes

Percidae

Etheostoma kantuckeense

highland rim darter

Perciformes

Percidae

Etheostoma brevirostrum

holiday darter

Perciformes

Percidae

Etheostoma exile

iowa darter

Perciformes

Percidae

Etheostoma nigrum

johnny darter

Perciformes

Percidae

Etheostoma kanawhae

kanawha darter

Perciformes

Percidae

Etheostoma rafinesquei

kentucky darter

Perciformes

Percidae

Etheostoma microperca

least darter

Perciformes

Percidae

Etheostoma chuckwachatte

lipstick darter


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Perciformes

Percidae

Etheostoma neopterum

lollypop darter

Perciformes

Percidae

Etheostoma longimanum

longfin darter

Perciformes

Percidae

Etheostoma sellare

maryland darter

Perciformes

Percidae

Etheostoma tetrazonum

missouri saddled darter

Perciformes

Percidae

Etheostoma asprigene

mud darter

Perciformes

Percidae

Etheostoma nianguae

niangua darter

Perciformes

Percidae

Etheostoma okaloosae

okaloosa darter

Perciformes

Percidae

Etheostoma radiosum

orangebelly darter

Perciformes

Percidae

Etheostoma bellum

orangefin darter

Perciformes

Percidae

Etheostoma spectabile

orangethroat darter

Perciformes

Percidae

Etheostoma pallididorsum

paleback darter

Perciformes

Percidae

Etheostoma mariae

pinewoods darter

Perciformes

Percidae

Etheostoma caeruleum

rainbow darter

Perciformes

Percidae

Etheostoma luteovinctum

redband darter

Perciformes

Percidae

Etheostoma whipplei

redfin darter

Perciformes

Percidae

Etheostoma rufilineatum

redline darter

Perciformes

Percidae

Etheostoma artesiae

redspot darter

Perciformes

Percidae

Etheostoma chienense

relict darter

Perciformes

Percidae

Etheostoma grahami

rio grande darter

Perciformes

Percidae

Etheostoma podostemone

riverweed darter

Perciformes

Percidae

Etheostoma rupestre

rock darter

Perciformes

Percidae

Etheostoma phytophilum

rush darter

Perciformes

Percidae

Etheostoma flavum

saffron darter

Perciformes

Percidae

Etheostoma fricksium

savannah darter

Perciformes

Percidae

Etheostoma serrifer

sawcheek darter

Perciformes

Percidae

Etheostoma thalassinum

seagreen darter

Perciformes

Percidae

Etheostoma acuticeps

sharphead darter

Perciformes

Percidae

Etheostoma tecumsehi

shawnee darter

Perciformes

Percidae

Etheostoma smithi

slabrock darter

Perciformes

Percidae

Etheostoma boschungi

slackwater darter

Perciformes

Percidae

Etheostoma gracile

slough darter

Perciformes

Percidae

Etheostoma microlepidum

smallscale darter

Perciformes

Percidae

Etheostoma simoterum

snubnose darter

Perciformes

Percidae

Etheostoma olivaceum

sooty darter

Perciformes

Percidae

Etheostoma stigmaeum

speckled darter

Perciformes

Percidae

Etheostoma barrenense

splendid darter

Perciformes

Percidae

Etheostoma squamiceps

spottail darter

Perciformes

Percidae

Etheostoma maculatum

spotted darter

Perciformes

Percidae

Etheostoma punctulatum

stippled darter

Perciformes

Percidae

Etheostoma derivativum

stone darter

Perciformes

Percidae

Etheostoma fragi

strawberry darter

Perciformes

Percidae

Etheostoma striatulum

striated darter


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Perciformes

Percidae

Etheostoma virgatum

striped darter

Perciformes

Percidae

Etheostoma kennicotti

stripetail darter

Perciformes

Percidae

Etheostoma fusiforme

swamp darter

Perciformes

Percidae

Etheostoma swannanoa

swannanoa darter

Perciformes

Percidae

Etheostoma tallapoosae

tallapoosa darter

Perciformes

Percidae

Etheostoma barbouri

teardrop darter

Perciformes

Percidae

Etheostoma olmstedi

tessellated darter

Perciformes

Percidae

Etheostoma tippecanoe

tippecanoe darter

Perciformes

Percidae

Etheostoma lachneri

tombigbee darter

Perciformes

Percidae

Etheostoma trisella

trispot darter

Perciformes

Percidae

Etheostoma gutselli

tuckasegee darter

Perciformes

Percidae

Etheostoma inscriptum

turquoise darter

Perciformes

Percidae

Etheostoma tuscumbia

tuscumbia darter

Perciformes

Percidae

Etheostoma douglasi

tuskaloosa darter

Perciformes

Percidae

Etheostoma variatum

variegate darter

Perciformes

Percidae

Etheostoma chermocki

vermilion darter

Perciformes

Percidae

Etheostoma perlongum

waccamaw darter

Perciformes

Percidae

Etheostoma bellator

warrior darter

Perciformes

Percidae

Etheostoma nuchale

watercress darter

Perciformes

Percidae

Etheostoma vulneratum

wounded darter

Perciformes

Percidae

Etheostoma raneyi

yazoo darter

Perciformes

Percidae

Etheostoma moorei

yellowcheek darter

Perciformes

Percidae

Etheostoma juliae

yoke darter

Perciformes

Percidae

Etheostoma meadiae

bluespar darter

Perciformes

Percidae

Etheostoma planasaxatile

duck darter

Perciformes

Percidae

Etheostoma orientale

eastrim darter

Perciformes

Percidae

Etheostoma occidentale

westrim darter

Perciformes

Percidae

Gymnocephalus cernuus

ruffe

Perciformes

Percidae

Perca flavescens

yellow perch

Perciformes

Percidae

Percina antesella

amber darter

Perciformes

Percidae

Percina gymnocephala

appalachia darter

Perciformes

Percidae

Percina macrolepida

bigscale logperch

Perciformes

Percidae

Percina nigrofasciata

blackbanded darter

Perciformes

Percidae

Percina maculata

blackside darter

Perciformes

Percidae

Percina burtoni

blotchside logperch

Perciformes

Percidae

Percina cymatotaenia

bluestripe darter

Perciformes

Percidae

Percina palmaris

bronze darter

Perciformes

Percidae

Percina nevisense

chainback darter

Perciformes

Percidae

Percina copelandi

channel darter

Perciformes

Percidae

Percina brevicauda

coal darter

Perciformes

Percidae

Percina jenkinsi

conasauga logperch

Perciformes

Percidae

Percina sclera

dusky darter


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Perciformes

Percidae

Percina stictogaster

frecklebelly darter

Perciformes

Percidae

Per tin a lenticula

freckled darter

Perciformes

Percidae

Percina evides

gilt darter

Perciformes

Percidae

Percina aurolineata

goldline darter

Perciformes

Percidae

Percina suttkusi

gulf logperch

Perciformes

Percidae

Percina pantherina

leopard darter

Perciformes

Percidae

Percina caprodes

logperch

Perciformes

Percidae

Percina macrocephala

longhead darter

Perciformes

Percidae

Percina nasuta

longnose darter

Perciformes

Percidae

Percina kathae

mobile logperch

Perciformes

Percidae

Percina squamata

olive darter

Perciformes

Percidae

Percina fulvitaenia

ozark logperch

Perciformes

Percidae

Percina aurora

pearl darter

Perciformes

Percidae

Percina crassa

piedmont darter

Perciformes

Percidae

Percina shumardi

river darter

Perciformes

Percidae

Percina roan oka

roanoke darter

Perciformes

Percidae

Percina rex

roanoke logperch

Perciformes

Percidae

Percina vigil

saddleback darter

Perciformes

Percidae

Percina oxyrhynchus

sharpnose darter

Perciformes

Percidae

Percina peltata

shield darter

Perciformes

Percidae

Percina phoxocephala

slenderhead darter

Perciformes

Percidae

Percina tanasi

snail darter

Perciformes

Percidae

Percina austroperca

southern logperch

Perciformes

Percidae

Percina uranidea

stargazing darter

Perciformes

Percidae

Percina notogramma

stripeback darter

Perciformes

Percidae

Percina aurantiaca

tangerine darter

Perciformes

Percidae

Percina carbonaria

texas logperch

Perciformes

Percidae

Percina burtoni

blotchside darter

Perciformes

Percidae

Sander canadensis

sauger

Perciformes

Percidae

Sander vitreus

walleye

Perciformes

Percidae

Sander lucioperca

zander

Perciformes

Percidae

Sander canadensis x vitreus

saugeye

Perciformes

Pomatomidae

Pomatomus saltatrix

bluefish

Perciformes

Sciaenidae

Aplodinotus grunniens

freshwater drum

Perciformes

Sciaenidae

Bairdiella icistia

bairdiella

Perciformes

Sciaenidae

Bairdiella chrysoura

silver perch

Perciformes

Sciaenidae

Cynoscion xanthulus

orangemouth corvina

Perciformes

Sciaenidae

Cynoscion nebulosus

spotted seatrout

Perciformes

Sciaenidae

Leiostomus xanthurus

spot

Perciformes

Sciaenidae

Micropogonias undulatus

atlantic croaker

Perciformes

Sciaenidae

Sciaenops ocellatus

red drum

Perciformes

Sparidae

Archosargus probatocephalus

sheepshead


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY



SCIENTIFIC NAME

COMMON NAME

Perciformes

Sparidae



Lagodon rhomboides

pinfish

Perciformes

Centrarchidae

Micropterus salmoides

largemouth bass (yoy)

Perciformes

Centropomidae

Centropomus parallel us

fat snook

Perciformes

Percidae



Etheostoma tennesseense

tennessee darter

Percopsiformes

Amblyopsidae

Amblyopsis spelaea

northern cavefish

Percopsiformes

Amblyopsidae

Amblyopsis rosae

ozark cavefish

Percopsiformes

Amblyopsidae

Chologaster cornuta

swampfish

Percopsiformes

Amblyopsidae

Forbesichthys agassizii

spring cavefish

Percopsiformes

Amblyopsidae

Speoplatyrhinus poulsoni

alabama cavefish

Percopsiformes

Amblyopsidae

Typhlichthys subterraneus

southern cavefish

Percopsiformes

Aphredoderidae

Aphredoderus sayanus

pirate perch

Percopsiformes

Percopsidae



Percopsis transmontana

sand roller

Percopsiformes

Percopsidae



Percopsis omiscomaycus

trout-perch

Petromyzontiformes

Petromyzont

dae

Ichthyomyzon castaneus

chestnut lamprey

Petromyzontiformes

Petromyzont

dae

Ichthyomyzon greet eyi

mountain brook lamprey

Petromyzontiformes

Petromyzont

dae

Ichthyomyzon fossor

northern brook lamprey

Petromyzontiformes

Petromyzont

dae

Ichthyomyzon bdellium

ohio lamprey

Petromyzontiformes

Petromyzont

dae

Ichthyomyzon unicuspis

silver lamprey

Petromyzontiformes

Petromyzont

dae

Ichthyomyzon gagei

southern brook lamprey

Petromyzontiformes

Petromyzont

dae

Lampetra appendix

american brook lamprey

Petromyzontiformes

Petromyzont

dae

Lampetra camtschatica

arctic lamprey

Petromyzontiformes

Petromyzont

dae

Lampetra hubbsi

kern brook lamprey

Petromyzontiformes

Petromyzont

dae

Lampetra similis

klamath lamprey

Petromyzontiformes

Petromyzont

dae

Lampetra aepyptera

least brook lamprey

Petromyzontiformes

Petromyzont

dae

Lampetra minima

miller lake lamprey

Petromyzontiformes

Petromyzont

dae

Lampetra tridentata

pacific lamprey

Petromyzontiformes

Petromyzont

dae

Lampetra lethophaga

pit-klamath brook lamprey

Petromyzontiformes

Petromyzont

dae

Lampetra ayresii

river lamprey

Petromyzontiformes

Petromyzont

dae

Lampetra richardsoni

western brook lamprey

Petromyzontiformes

Petromyzont

dae

Petromyzon marinus

sea lamprey

Petromyzontiformes

Petromyzont

dae

Lampetra similis

klamath river lamprey

Pleuronectiformes

Achiridae



Trinectes maculatus

hogchoker

Pleuronectiformes

Paralichthyidae

Citharichthys spilopterus

bay whiff

Pleuronectiformes

Paralichthyidae

Paralichthys lethostigma

southern flounder

Pleuronectiformes

Pleuronectidae

Platichthys stellatus

starry flounder

Pristiformes

Pristidae



Pristis pectinata

smalltooth sawfish

Salmoniformes

Osmeridae



Hypomesus transpacificus

delta smelt

Salmoniformes

Osmeridae



Hypomesus olidus

pond smelt

Salmoniformes

Osmeridae



Hypomesus pretiosus

surf smelt

Salmoniformes

Osmeridae



Hypomesus nipponensis

wakasagi

Salmoniformes

Osmeridae



Osmerus mordax

rainbow smelt

Salmoniformes

Osmeridae



Spirinchus thaleichthys

longfin smelt


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Salmoniformes

Osmeridae

Thaleichthys pacificus

eulachon

Salmoniformes

Salmonidae

Coregonus autumnalis

arctic cisco

Salmoniformes

Salmonidae

Coregonus laurettae

bering cisco

Salmoniformes

Salmonidae

Coregonus nigripinnis

blackfin cisco

Salmoniformes

Salmonidae

Coregonus hoyi

bloater

Salmoniformes

Salmonidae

Coregonus nasus

broad whitefish

Salmoniformes

Salmonidae

Coregonus artedi

cisco

Salmoniformes

Salmonidae

Coregonus johannae

deepwater cisco

Salmoniformes

Salmonidae

Coregonus pidschian

humpback whitefish

Salmoniformes

Salmonidae

Coregonus kiyi

kiyi

Salmoniformes

Salmonidae

Coregonus clupeaformis

lake whitefish

Salmoniformes

Salmonidae

Coregonus sard in el la

least cisco

Salmoniformes

Salmonidae

Coregonus zenithicus

shortjaw cisco

Salmoniformes

Salmonidae

Coregonus reighardi

shortnose cisco

Salmoniformes

Salmonidae

Oncorhynchus tshawytscha

Chinook salmon

Salmoniformes

Salmonidae

Oncorhynchus keta

chum salmon

Salmoniformes

Salmonidae

Oncorhynchus kisutch

coho salmon

Salmoniformes

Salmonidae

Oncorhynchus clarkii

cutthroat trout

Salmoniformes

Salmonidae

Oncorhynchus gilae

gila trout

Salmoniformes

Salmonidae

Oncorhynchus gorbuscha

pink salmon

Salmoniformes

Salmonidae

Oncorhynchus mykiss

rainbow trout

Salmoniformes

Salmonidae

Oncorhynchus nerka

sockeye salmon

Salmoniformes

Salmonidae

Oncorhynchus clarkii Utah

bonneville cutthroat trout

Salmoniformes

Salmonidae

Oncorhynchus clarkii lewisi

westslope cutthroat trout

Salmoniformes

Salmonidae

Oncorhynchus apache x

apache x rainbow trout





mykiss



Salmoniformes

Salmonidae

Oncorhynchus mykiss x

rainbow x golden trout





aguabonita



Salmoniformes

Salmonidae

Prosopium abyssicola

bear lake whitefish

Salmoniformes

Salmonidae

Prosopium gemmifer

bonneville cisco

Salmoniformes

Salmonidae

Prosopium spilonotus

bonneville whitefish

Salmoniformes

Salmonidae

Prosopium williamsoni

mountain whitefish

Salmoniformes

Salmonidae

Prosopium coulterii

pygmy whitefish

Salmoniformes

Salmonidae

Prosopium cylindraceum

round whitefish

Salmoniformes

Salmonidae

Salmo salar

atlantic salmon

Salmoniformes

Salmonidae

Salmo trutta

brown trout

Salmoniformes

Salmonidae

Salmo X Salvelinus trutta x

tiger trout





fontinalis



Salmoniformes

Salmonidae

Salvelinus a 1 pin us

arctic char

Salmoniformes

Salmonidae

Salvelinus fontinalis

brook trout

Salmoniformes

Salmonidae

Salvelinus confluentus

bull trout

Salmoniformes

Salmonidae

Salvelinus malma

dolly varden


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

<
CO

<
Q

<
I

u_
u_

O

U~)
LU

<
u

u

i/l

Q
Z
<

o

o
u

Q
QC
<
Q

<
I—

i/l

Q
X
Q

CL
CL
<

Salmoniformes

Salmonidae

Salvelinus namaycush

lake trout

Salmoniformes

Salmonidae

Stenodus leucichthys

inconnu

Salmoniformes

Salmonidae

Thymallus arcticus

arctic grayling

Salmoniformes

Salmonidae

Oncorhynchus tshawytscha

Chinook salmon (yoy)

Salmoniformes

Salmonidae

Oncorhynchus clarkii clarkii

coastal cutthroat trout

Salmoniformes

Salmonidae

Oncorhynchus clarkii

Colorado river cutthroat trout





pleuriticus



Salmoniformes

Salmonidae

Oncorhynchus clarkii x mykiss

cutbow

Salmoniformes

Salmonidae

Oncorhynchus clarkii

lahontan cutthroat trout





henshawi



Salmoniformes

Salmonidae

Oncorhynchus mykiss

rainbow trout (steelhead)

Salmoniformes

Salmonidae

Oncorhynchus mykiss

redband rainbow trout





gairdneri



Salmoniformes

Salmonidae

Oncorhynchus aguabonita

golden trout

Salmoniformes

Salmonidae

Salmo salar

atlantic salmon juvenile

Scorpaeniformes

Cottidae

Clinocottus acuticeps

sharpnose sculpin

Scorpaeniformes

Cottidae

Cottus carolinae

banded sculpin

Scorpaeniformes

Cottidae

Cottus extensus

bear lake sculpin

Scorpaeniformes

Cottidae

Cottus baileyi

black sculpin

Scorpaeniformes

Cottidae

Cottus caeruleomentum

blue ridge sculpin

Scorpaeniformes

Cottidae

Cottus aleuticus

coastrange sculpin

Scorpaeniformes

Cottidae

Cottus hubbsi

Columbia sculpin

Scorpaeniformes

Cottidae

Cottus princeps

klamath lake sculpin

Scorpaeniformes

Cottidae

Cottus bendirei

malheur sculpin

Scorpaeniformes

Cottidae

Cottus klamathensis

marbled sculpin

Scorpaeniformes

Cottidae

Cottus marginatus

margined sculpin

Scorpaeniformes

Cottidae

Cottus bairdii

mottled sculpin

Scorpaeniformes

Cottidae

Cottus hypselurus

ozark sculpin

Scorpaeniformes

Cottidae

Cottus beldingii

paiute sculpin

Scorpaeniformes

Cottidae

Cottus pitensis

pit sculpin

Scorpaeniformes

Cottidae

Cottus girardi

potomac sculpin

Scorpaeniformes

Cottidae

Cottus asper

prickly sculpin

Scorpaeniformes

Cottidae

Cottus paulus

pygmy sculpin

Scorpaeniformes

Cottidae

Cottus perplexus

reticulate sculpin

Scorpaeniformes

Cottidae

Cottus gulosus

riffle sculpin

Scorpaeniformes

Cottidae

Cottus asperrimus

rough sculpin

Scorpaeniformes

Cottidae

Cottus confusus

shorthead sculpin

Scorpaeniformes

Cottidae

Cottus greenei

shoshone sculpin

Scorpaeniformes

Cottidae

Cottus tenuis

slender sculpin

Scorpaeniformes

Cottidae

Cottus cognatus

slimy sculpin

Scorpaeniformes

Cottidae

Cottus ricei

spoonhead sculpin

Scorpaeniformes

Cottidae

Cottus rhotheus

torrent sculpin

D-24


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Scorpaeniformes

Cottidae

Cottus echinatus

utah lake sculpin

Scorpaeniformes

Cottidae

Cottus leiopomus

wood river sculpin

Scorpaeniformes

Cottidae

Cottus Chattahoochee

Chattahoochee sculpin

Scorpaeniformes

Cottidae

Cottus cf. broadband sculpin

clinch sculpin

Scorpaeniformes

Cottidae

Cottus tallapoosae

tallapoosa sculpin

Scorpaeniformes

Cottidae

Leptocottus armatus

pacific staghorn sculpin

Scorpaeniformes

Cottidae

Myoxocephalus thompsonii

deepwater sculpin

Scorpaeniformes

Cottidae

Myoxocephalus quadricornis

fourhorn sculpin

Scorpaeniformes

Cottidae

Cottus kanawhae

kanawha sculpin

S

luri

ormes

Ariidae

Ariopsis felis

hardhead catfish

S

luri

ormes

Callichthyidae

Hoplosternum littorale

brown hoplo

S

luri

ormes

Clariidae

Clarias batrachus

walking catfish

S

luri

ormes

Doradidae

Platydoras armatulus

southern striped raphael

S

luri

ormes

Ictaluridae

Ameiurus melas

black bullhead

S

luri

ormes

Ictaluridae

Ameiurus nebulosus

brown bullhead

S

luri

ormes

Ictaluridae

Ameiurus platycephalus

flat bullhead

S

luri

ormes

Ictaluridae

Ameiurus brunneus

snail bullhead

S

luri

ormes

Ictaluridae

Ameiurus serracanthus

spotted bullhead

S

luri

ormes

Ictaluridae

Ameiurus catus

white catfish

S

luri

ormes

Ictaluridae

Ameiurus natalis

yellow bullhead

S

luri

ormes

Ictaluridae

Ictalurus furca tus

blue catfish

S

luri

ormes

Ictaluridae

Ictalurus punctatus

channel catfish

S

luri

ormes

Ictaluridae

Ictalurus lupus

headwater catfish

S

luri

ormes

Ictaluridae

Ictalurus pricei

yaqui catfish

S

luri

ormes

Ictaluridae

Noturus funebris

black madtom

S

luri

ormes

Ictaluridae

Noturus miurus

brindled madtom

S

luri

ormes

Ictaluridae

Noturus phaeus

brown madtom

S

luri

ormes

Ictaluridae

Noturus taylori

caddo madtom

S

luri

ormes

Ictaluridae

Noturus furiosus

Carolina madtom

S

luri

ormes

Ictaluridae

Noturus flavater

checkered madtom

S

luri

ormes

Ictaluridae

Noturus elegans

elegant madtom

S

luri

ormes

Ictaluridae

Noturus munitus

frecklebelly madtom

S

luri

ormes

Ictaluridae

Noturus nocturnus

freckled madtom

S

luri

ormes

Ictaluridae

Noturus hildebrandi

least madtom

S

luri

ormes

Ictaluridae

Noturus insignis

margined madtom

S

luri

ormes

Ictaluridae

Noturus eleutherus

mountain madtom

s

luri

ormes

Ictaluridae

Noturus placidus

neosho madtom

s

luri

ormes

Ictaluridae

Noturus stigmosus

northern madtom

s

luri

ormes

Ictaluridae

Noturus gilberti

orangefin madtom

s

luri

ormes

Ictaluridae

Noturus lachneri

ouachita madtom

s

luri

ormes

Ictaluridae

Noturus albater

ozark madtom

s

luri

ormes

Ictaluridae

Noturus stanauli

pygmy madtom


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Siluriformes

Ictaluridae

Noturus trautmani

scioto madtom

Siluriformes

Ictaluridae

Noturus exilis

slender madtom

Siluriformes

Ictaluridae

Noturus baileyi

smoky madtom

Siluriformes

Ictaluridae

Noturus leptacanthus

speckled madtom

Siluriformes

Ictaluridae

Noturus flavus

stonecat

Siluriformes

Ictaluridae

Noturus gyrinus

tadpole madtom

Siluriformes

Ictaluridae

Noturus flavipinnis

yellowfin madtom

Siluriformes

Ictaluridae

Pylodictis olivaris

flathead catfish

Siluriformes

Ictaluridae

Satan eurystomus

widemouth blindcat

Siluriformes

Ictaluridae

Trogloglanis pattersoni

toothless blindcat

Siluriformes

Loricariidae

Hypostomus plecostomus

suckermouth catfish

Siluriformes

Loricariidae

Pterygoplichthys pa rd a lis

amazon sailfin catfish

Siluriformes

Loricariidae

Pterygoplich thys m ultiradiatus

Orinoco sailfin catfish

Siluriformes

Loricariidae

Pterygoplichthys anisitsi

southern sailfin catfish

Siluriformes

Loricariidae

Pterygoplich thys disjun ctivus

vermiculated sailfin catfish

Siluriformes

Ictaluridae

No turus fascia tus

saddled madtom

Synbranchiformes

Synbranchidae

Monopterus albus

asian swamp eel


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National Rivers and Streams Assessment 2018/19	Field Operations Manual

Version 1.2 May 2018

Table D-2. Standard Common and Scientific Names of Amphibians1

ORDER

FAMILY

SCIENTIFIC NAME

COMMON NAME

Anura

Ascaphidae

Ascaphus truei

tailed frog

Anura

Ascaphidae

Ascaphus truei

tailed frog (tadpole)

Anura

Bufonidae

Bufo boreas

western toad

Anura

Bufonidae

Bufo microscaphus

arizona toad

Anura

Bufonidae

Bufo hemiophrys

Canadian toad

Anura

Bufonidae

Bufo punctatus

red-spotted toad

Anura

Bufonidae

Bufo woodhousii

woodhouse's toad

Anura

Hylidae

Hyla arenicolor

canyon treefrog

Anura

Hylidae

Pseudacris maculata

boreal chorus frog

Anura

Hylidae

Pseudacris cadaverina

California treefrog

Anura

Hylidae

Pseudacris regilla

pacific tree frog

Anura

Pipidae

Xenopus laevis

african clawed frog

Anura

Ranidae

Lithobates pipiens

leopard frog

Anura

Ranidae

Lithobates blairi

plains leopard frog

Anura

Ranidae

Lithobates chiricahuensis

chiricahua leopard frog

Anura

Ranidae

Rana catesbeiana

bullfrog

Anura

Ranidae

Rana catesbeiana

bullfrog tadpole

Anura

Ranidae

Rana aurora

red-legged frog

Anura

Ranidae

Rana cascadae

cascade frog

Anura

Ranidae

Rana luteiventris

Columbia spotted frog

Anura

Ranidae

Rana boylii

foothill yellow-legged frog

Anura

Ranidae

Rana clamitans

green frog

Anura

Ranidae

Rana yavapaiensis

lowland leopard frog

Anura

Ranidae

Rana muscosa

mountain yellow-legged frog

Anura

Ranidae

Rana pretiosa

spotted frog

Anura

Ranidae

Rana sylvatica

wood frog

Caudata

Ambystomatidae

Ambystoma macrodactylum

longtoed salamander

Caudata

Ambystomatidae

Dicamptodon tenebrosus

pacific giant salamander

Caudata

Ambystomatidae

Dicamptodon ensatus

California giant salamander

Caudata

Ambystomatidae

Dicamptodon aterrimus

idaho giant salamander

Caudata

Rhyacotritonidae

Rhyacotriton kezeri

Columbia torrent salamander

Caudata

Salamandridae

Taricha granulosa

rough-skinned newt

Caudata

Salamandridae

Taricha torosa

California newt

1 Although not required, you may note amphibians and reptiles captured on the fish collection form.


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Appendix E: Example
Electrofishing Settings

i/i

i/i

o

QC
I—

u

<
X

X
Q

CL
CL
<

E-l


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Example Backpack Output Goals

Waveform: PDC (fish catching settings, e.g., 60 pps, 25% duty cycle)
Electrodes: Ring anode, rattail cathode
R100:	275 Ohms

Fish Cond.: 115 piS/cm

Water
Conductivity (Ambient)

Applied Voltage
Goal (Peak)

Applied Amperage
Goal (Peak)

25

669

0.61

50

394

0.72

60

349

0.76

80

291

0.85

100

257

0.93

150

211

1.15

200

188

1.37

250

174

1.59

300

165

1.80

400

154

2.24

500

147

2.67

600

142

3.11

700

139

3.54

800

137

3.98

900

135

4.41

1000

133

4.85

1500

129

7.02

2000

126

9.20

2500

125

11.37

3000

124

13.54

Adapted from:

http://electrofishing.net/2018/01/ll/output-goal-tables-for-backpacks-towbarges-and-boats/

If your conductivity meter cannot measure ambient conductivity, you can "uncorrect" specific
conductance at 25 °C to ambient conductivity using the following equation:

• Ambient conductivity=Specific conductance x (l+([water temp-25 °C] x 0.02))


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Field Operations Manual

Example Towed-barge Output Goal Table (1 anode)

Waveform: 80 pps, 40% duty cycle

Anode:	One Ring 14" diameter (35.56 cm)

Cathode:	Plate 66" x 45" (167.6 cm x 114 cm)

R100:	147 Ohms with one anode

Fish Cond.:	115 piS/cm

Water

Applied Voltage

Applied Amperage

Conductivity (Ambient)

Goal (Peak)

Goal (Peak)

25

921

1.58

50

543

1.87

60

480

1.98

80

401

2.20

100

354

2.43

150

291

3.00

200

259

3.56

250

240

4.13

300

228

4.69

400

212

5.82

500

202

6.95

600

196

8.08

700

192

9.21

800

188

10.34

900

186

11.47

1000

183

12.60

1500

177

18.26

2000

174

23.91

2500

172

29.56

3000

171

35.21

Adapted from:

http://electrofishing.net/2018/01/ll/output-goal-tables-for-backpacks-towbarges-and-boats/

If your conductivity meter cannot measure ambient conductivity, you can "uncorrect" specific
conductance at 25 °C to ambient conductivity using the following equation:

• Ambient conductivity=Specific conductance x (l+([water temp-25 °C] x 0.02))


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National Rivers and Streams Assessment 2018/19
Version 1.2 May 2018

Example 2-boom Boat Output Goal Table

Waveform:	PDC (fish catching settings, e.g., 60 pps, 25% duty cycle)

Anode:	Two booms Wisconsin rings 4-6 droppers

Cathode:	Boat hull (16 - 18' length)

R100:	35 Ohms

Fish Cond.:	115 piS/cm

Field Operations Manual

Water Conductivity

Applied Voltage

Applied Amperage

(Ambient)

Goal (Peak)

Goal (Peak)

25

851

6.09

50

502

7.17

60

443

7.61

80

371

8.48

100

327

9.35

150

269

11.52

200

239

13.70

250

222

15.87

300

210

18.04

400

196

22.39

500

187

26.74

600

181

31.09

700

177

35.43

800

174

39.78

900

171

44.13

1000

169

48.48

1500

164

70.22

2000

161

91.96

2500

159

113.70

3000

158

135.43

^ Adapted from:

1=

LU
l/l

http://electrofishing.net/2018/01/ll/output-goal-tables-for-backpacks-towbarges-and-boats/

If your conductivity meter cannot measure ambient conductivity, you can "uncorrect" specific
conductance at 25 °C to ambient conductivity using the following equation:

^	• Ambient conductivity=Specific conductance x (l+([water temp-25 °C] x 0.02))

O

OC Rationale for boats:

We are seeing from several sources that 10 - 12 amps are the required minimum for successful fishing at 115 |aS/cm with a 2-boom
y electrofishing boat using rectangular-wave pulsed DC. Rounded wave PDC, as produced from GPP units, appear to require higher amperages
^ (12 - 14 amp range at 115 |iS/cm). The above table uses a 35 Ohm boat electrode system (2 booms with 4-6 droppers each, unpainted boat

hull) and the low end of the rectangular wave requirements (10 amps at 115 |iS/cm).

^ The best output to standardize by is current. However, in low conductivity water, or when using low power units as backpacks, voltage may be
^ a better choice to use instead of current.

>< Here's the big catch. If you plan to use these as starting guides, then the electrode arrangement should be similar to that described for each
table. For example, spherical boat electrodes are not similar enough to Wisconsin Rings for this purpose. We all know that electrode

111

^ arrangements will differ (different length hulls, 4 droppers instead of 6, etc. The cool thing is that current targets will be more consistent while
q voltage targets will vary more.

Z For a good blog with data on this concept, see:

http://electrofishing.net/2016/05/21/boat-fleet-fishing-thresholds/

^ For information on different boat and raft electrode arrangements, next look at this:

http://electrofishing.net/2017/03/20/estimating-electrofishing-thresholdswithout-fish/

E-4


-------