&EPA

United States
Environmental
Protection Agency

Office of Research
and Development
Washington, DC 20460

EPA A620/R-01/004h
March 2003

EMAP-West Communications

Indices of Biotic integrity

The Clean Water Act calls for the "restoration and maintenance of
biological integrity of Nation's waters." To fulfill this require-
ment, environmental managers must be able to measure the
biological condition of their aquatic resources. EMAP-West is
helping to meet this need by developing tools for assessing condi-
tion known as Indices of Biotic Integrity (IBI's), extending the
work of James Karr who first developed IBI's to measure the
condition of fish assemblages in the early 1980s. The IBI concept
has since been extended to other types of stream organisms
(including periphyton - algae that are attached to the stream
bottom, and invertebrates - insects, both of which serve as valua-
ble indicators of the biotic condition). EMAP-West scientists and
cooperators are developing this concept further for streams across
the 12-state region of the Program.

The IBI concept has developed around the assumption that similar
groups or categories of organisms respond similarly to human-
induced changes in their enviromnent. Based on this assumption
IBI development requires that a set of metrics of the integrity of
the assemblage of interest (e.g., fish) be identified and calibrated.
Examples of candidate metrics for EMAP-West are:

•	species richness (e.g., total number of species, or percent
alien species),

•	habitat preferences (e.g., percent of the species that live on
the stream bottom),

•	mode of reproduction (e.g., percent of individuals that spawn
on clean gravel),

•	tolerance categories (e.g., number of species which are intol-
erant to chemical disturbance).

Evaluating the statistical quality of the candidate metrics, their
redundancy with other candidate metrics, and responsiveness to
disturbance initially screens candidate metrics. Remaining candi-
date metrics that are responsive to natural factors, such as stream
size, are adjusted to account for these natural factors. The result is
that changes in the candidate metrics reflect human-induced
changes, rather than natural variability.

Scores are then assigned to the screened and adjusted metrics for
each sampled location. Higher scores (usually a maximum of 10)
are assigned for locations where the metric value more closely
matches the value of the metric at reference sites. The scores for
the metrics are added together resulting in an IBI score for each
site, based on multiple metrics. The index is then validated by
testing the response of the IBI scores for a set of locations with
known human-induced stressors. The probability sampling design
that determines how the sample sites are selected allows us to
describe the biotic integrity of a population of streams in a region
of interest.

Wherever sufficient data are available, EMAP-West scientists and
cooperators will develop IBI's for fish, invertebrates, and algae.
Each cooperating state and tribe will then be able to assess the
biological condition of their aquatic resources and, through appro-
priate management actions, move closer to meeting the require-
ments of the Clean Water Act.

For further information, contact:

John Stoddard
Surface Waters Lead

Stoddard.John@epa.gov
(541)754-4441


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