Effects of Concentrated Animal Feeding Operations (CAFOs)

on Ground Water Quality

Research Type and Organization

The research type is in-house. The organization is EPAs Ground
Water and Ecosystem Restoration Division.

Project Period

January 1, 2000 to present

Project Summary

Research is underway at EPA's Ground Water and Ecosystems
Restoration Division to evaluate the effect of concentrated animal
feeding operations (CAFOs) on ground water quality following land
application. The overall research objectives are to:

•	Characterize the potential impact on ground water from a variety of stressors associated with different types
of CAFOs

•	Develop tools to determine sources and specific mechanisms of impact

•	Collectively use these data to develop effective risk-management strategies for protection of ground water
quality

Initial specific research objectives were to evaluate selected swine
CAFO operations to understand which stressors pose the greatest risk
for transport into ground water under site operating conditions. Three
CAFOs were selected for study: a new farrowing sow operation, an
existing nursery operation, and a closed combined facility.

For the sow and combined facilities, ground water samples were
simply obtained from existing wells and analyzed for standard water
quality indices as well as total organic carbon, nutrients,
cations/metals, pharmaceutical chemicals, estrogens, and pathogen
indicator organisms. The nursery field site study was much more
extensive; site characterization included core acquisition and logging,

Geoprobe electrical conductivity logging, completion and monitoring
of numerous cluster wells, stable isotope studies, slug testing, and
other activities over an extended period.

Results show that land application of liquid swine waste at the nursery site led to ground water contam ination by
nitrate (a manuscript is in progress). Other stressors, such as estrogen hormones (which are endocrine-disrupting
chemicals, or EDCs), were not detected in ground water at that site. Flowever, estrogens as well as nitrate and
ammonia were found in ground water at the closed combined facility which had been directly impacted by a
leaking lagoon (a manuscript is in progress).

The National Risk Management Research Laboratory's mission is to advance scientific and engineering
solutions that enable EPA and others to effectively manage current and future environmental risks.
NRMRL possesses unique strengths and capabilities and is dedicated to providing credible
technological information and scientific solutions that support national priorities
and protect human health and the environment.


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Based on these studies, secondary research objectives have been identified and research is in progress to include
other types of CAFOs and to provide additional focus on other hormones that act as EDCs. EPA's initial field site
work was facilitated by close coordination with EPA Region 6 personnel. This collaboration will continue in the
identification and evaluation of appropriate field sites. In addition, through EPA's Science to Achieve Results
Grants Program, EPA personnel will be collaborating with university personnel on CAFO research at a beef field
site (University of Nebraska-Lincoln) and a poultry field site (University of Maryland).

Products

Bradford, S.A., E. Segal, W. Zheng, Q. Wang, and S.R. Hutchins. (2008). "Reuse of Concentrated Animal Feeding
Operation Wastewater on Agricultural Lands."./, Environ. Qual., 37: 1-19.

Hutchins, S.R., M.V. White, F.M. Hudson, and D.D. Fine. (2007). "Analysis of Lagoon Samples From Different
Concentrated Animal Feeding Operations for Estrogens and Estrogen Conjugates." Environ. Sci. Technol., 41: 738-
744.

Lazorchak, J.M., S.R. Hutchins, G.T. Ankley, and M.A. Mills. (2005). "Overview of Field Research on EDCs From
Concentrated Animal Feeding Operations - NERL Molecular Diagnostics; NHEERL Toxicity, Analytical
Chemistry and Effects Measurements; NRMRL Risk Management Characterization." (EPA/600/R-05/155)

Fine, D.D., G.P. Breidenbach, T.L. Price, and S.R. Hutchins. (2003). "Quantitation of Estrogens in Groundwater
and Swine Lagoon Samples Using Solid-Phase Extraction, Pentafluorobenzyl/Trimethylsilyl Derivitizations and
Gas Chromatography-Negative Ion Chemical Ionization Tandem Mass Spectrometry."./, Chromatogr., 1017: 167—
185.

Hutchins, S.R., M.V. White, D.D. Fine, and G.P. Breidenbach. (2003). "Analysis of Swine Lagoons and Ground
Water for Environmental Estrogens." In In Situ and On-Site Bioremediation. Edited by V.S. Magar and M.E.

Kelley. Battelle Press, Columbus, Ohio.

Contact

Steve Hutchins

The National Risk Management Research Laboratory's mission is to advance scientific and engineering
solutions that enable EPA and others to effectively manage current and future environmental risks.
NRMRL possesses unique strengths and capabilities and is dedicated to providing credible
technological information and scientific solutions that support national priorities
and protect human health and the environment.


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