SEPA NRMRL

\ NATIONAL RISK MANAGEMENT RESEARCH LABORATORY
www.epa.gov/nrmrl GROUND WATER AND ECOSYSTEMS RESTORATION RESEARCH

Transport and Fate of Nitrate and Pathogens at a Dairy Lagoon
Water Application Site: An Assessment of CNMP Performance

Research Type and Organization

The research type is an Interagency Agreement
(DWI292189901) with the U.S. Salinity Laboratory and the
U.S. Department of Agriculture's Agriculture Research
Service.

Project Period

April 1, 2005 through September 30, 2009
Project Summary

EPA currently requires that application of concentrated
animal feeding operation wastes to agricultural fields
follows a Nutrient Management Plan (NMP). The tacit
assumption is that a well-designed and executed NMP ensures that all lagoon water contaminants (nutrients and
pathogens) are retained or taken up in the root zone so that ground water is inherently protected. The proposed
research is designed to test the assumption that appropriate NMPs are protective of ground water and to address
potential weaknesses in the land-application design and operation processes. A well-designed and managed CNMP
was implemented on 5-by-l 0-meter plots at a dairy farm in San Jacinto, California, for four years using different
forage and application patterns. The selected site was intensively characterized and instrumented for the
experimental studies. Lagoon water application rates were determined by following NMP protocols established in
consultation with the National Resource Conservation Service (NRCS).

Spatial and temporal variations in water, nutrient, and
indicator microbe levels at the site were determined using a
system of nested tensiometers/soil solution samplers,
neutron probe readings, drain gauges, and monitoring wells,
as well as periodic soil coring, plant tissue analysis, and
apparent soil electrical conductivity measurements. Along
with the field experiments, laboratory experiments were
also conducted, using microbes, lagoon water, well water,
and soils from the NMP field site. Microcosm and batch
studies were conducted to quantify microbial growth, death,
and inactivation, as well as equilibrium partitioning of
microbes at the solid-water and air-water interfaces.

Transport experiments were conducted to quantify the
influence of water content, microorganism size, grain-size
distribution, and lagoon water composition on the
movement and retention of microbes.

The National Risk Management Research Laboratory's mission is to advance scientific and engineering
solutions that enable EPA and others to effectively manage current and future environmental risks.
NRMRL possesses unique strengths and capabilities and is dedicated to providing credible
technological information and scientific solutions that support national priorities
and protect human health and the environment.


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Kinetic transport parameters were estimated by fitting
numerical simulations to experimental data. Nitrate and
indicator microbe transport and fate at the field site were
modeled using an unsaturated zone water flow and solute
transport model (HYDRUS ID and 2D) capable of
simulating preferential flow paths.

This project is completed and has resulted in multiple
journal articles that provide detailed information on the
various aspects of the project. A final EPA report is in
progress to summarize these results.

Products

Leij, F.J. and S.A. Bradford. (In review). "Combined
Physical and Chemical Nonequilibrium Transport Model:

Analytical Solution, Moments, and Application to Colloids/' J. Contain. Hydrol.

Segal, E., P. Shouse, J.A. Poss, D.M. Crohn, and S.A. Bradford. (In review). "'Strategies for Nutrient Management
Plans in a Semi-Arid Environment.".^gricul. Ecosys. Environ.

Bradford, S.A. and E. Segal. (2008). "Fate of Indicator Microorganisms Under Nutrient Management Plan
Conditions." J! Environ. Qual., 38: 1728-1738.

Bradford, S.A., E. Segal, W. Zheng, Q. Wang, and S.R. Hutchins. (2008). "Reuse of CAFO Wastewater on
Agricultural Lands.'' J. Environ. Qual., 37: S97-S115.

Bradford, S.A., S. Torkzaban, F. Leij, J. Simunek, and M.Th. van Genuchten. (2008), ^'Modeling the Coupled
Effects of Pore Space Geometry and Velocity on Colloid Transport and Retention." Water Resources Res., 45.
W02414, doi:10.1029/2008WR007096.

Segal, E., P. Shouse, and S.A. Bradford. (2008). "Deterministic Analysis and Upscaling of Bromide Transport in a
Fleterogeneous Vadose Zone." Vadose Zone J., 8: 601-610.

Segal, E., S.A. Bradford, P. Shouse, N. Lazarovitch, and D. Corwin. (2008). "Integration of Hard and Soft Data to
Characterize Field-Scale Hydraulic Properties for Flow and Transport Studies." Vadose Zone J., 7: 878-889.

Tazehkand, S.S., S. Torkzaban, S.A. Bradford, and S.L. Walker. (2008). "Cell Preparation Methods Influence E.
coli D2lg Surface Chemistry and Transport in Saturated Porous Media." J. Environ. Qual., 37: 2108-2115.

Torkzaban, S., S.A. Bradford, M.Th. van Genuchten, and S.L. Walker. (2008), "Colloid Transport in Unsaturated
Porous Media: The Role of Water Content and Ionic Strength on Particle Straining." J. Contam. Hydro!., 96: 113
127.

Zheng, W., S R. Yates, and S.A. Bradford. (2008), 'Analysis of Steroid Hormones in Dairy Manure and
Wastewaters." Environ. Sci. Techno/.. 42: 530-535.

Bradford, S.A. and N. Toride. (2007). "A Stochastic Model for Colloid Transport and Deposition." J. Environ
Qual, 36: 1346-1356.

Bradford, S.A., S. Torkzaban, and S.L. Walker. (2007). "Coupling of Physical and Chemical Mechanisms of
Colloid Straining in Saturated Porous Media." Water Res., 41: 3012-3024.

Contact

Scott Bradford

The National Risk Management Research Laboratory's mission is to advance scientific and engineering
solutions that enable EPA and others to effectively manage current and future environmental risks.
NRMRL possesses unique strengths and capabilities and is dedicated to providing credible
technological information and scientific solutions that support national priorities
and protect human health and the environment.

Work Shed

Blending -Wheitgnu (years 1-2)
Alfalfa (years 3 -1)

s

Fenee

7 Seniors
~ N Volitil. Chamber
Rkii Gauge.

) Culvert Pipes ^ Weather Station
I Lagoon Water

O Lysitneler
Evaporation Pin ¦ EC Probes
Soil core location / Ne»Urmi Access


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